LOCUS       DAJSBN010000001       148248 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJSBN010000001 DAJSBN010000000
VERSION     DAJSBN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 148248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 148248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..148248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.001"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(12..1535)
                     /locus_tag="ONT86_000001"
     CDS             complement(12..1535)
                     /locus_tag="ONT86_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR2350831.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="ONT86_000002"
     CDS             1787..2968
                     /locus_tag="ONT86_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2350832.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ONT86_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ONT86_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR2350833.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="ONT86_000004"
     CDS             complement(5053..7764)
                     /locus_tag="ONT86_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2350834.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="ONT86_000005"
     CDS             complement(7774..8475)
                     /locus_tag="ONT86_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2350835.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="ONT86_000006"
     CDS             8661..9260
                     /locus_tag="ONT86_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2350836.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="ONT86_000007"
     CDS             9341..10354
                     /locus_tag="ONT86_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR2350837.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="ONT86_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="ONT86_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR2350838.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="ONT86_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="ONT86_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR2350839.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="ONT86_000010"
     CDS             11802..13175
                     /locus_tag="ONT86_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2350840.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="ONT86_000011"
     CDS             13532..15676
                     /locus_tag="ONT86_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR2350841.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="ONT86_000012"
     CDS             complement(15728..16768)
                     /locus_tag="ONT86_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR2350842.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="ONT86_000013"
     CDS             complement(17045..18847)
                     /locus_tag="ONT86_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2350843.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="ONT86_000014"
     CDS             complement(18861..19613)
                     /locus_tag="ONT86_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2350844.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="ONT86_000015"
     CDS             complement(19622..19984)
                     /locus_tag="ONT86_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2350845.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="ONT86_000016"
     CDS             complement(20160..21032)
                     /locus_tag="ONT86_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR2350846.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="ONT86_000017"
     CDS             complement(21058..21570)
                     /locus_tag="ONT86_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCR2350847.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="ONT86_000018"
     CDS             complement(21708..22499)
                     /locus_tag="ONT86_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCR2350848.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="ONT86_000019"
     CDS             complement(22554..23162)
                     /locus_tag="ONT86_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCR2350849.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="ONT86_000020"
     CDS             complement(23155..23472)
                     /locus_tag="ONT86_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2350850.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="ONT86_000021"
     CDS             complement(23628..24356)
                     /locus_tag="ONT86_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR2350851.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="ONT86_000022"
     CDS             24515..25396
                     /locus_tag="ONT86_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2350852.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="ONT86_000023"
     CDS             complement(25633..26676)
                     /locus_tag="ONT86_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2350853.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLYFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="ONT86_000024"
     CDS             complement(26679..27347)
                     /locus_tag="ONT86_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2350854.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="ONT86_000025"
     CDS             27489..29825
                     /locus_tag="ONT86_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2350855.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="ONT86_000026"
     CDS             complement(29940..30251)
                     /locus_tag="ONT86_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2350856.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="ONT86_000027"
     CDS             30432..31907
                     /locus_tag="ONT86_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCR2350857.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="ONT86_000028"
     CDS             31954..32538
                     /locus_tag="ONT86_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR2350858.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="ONT86_000029"
     CDS             32540..32959
                     /locus_tag="ONT86_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCR2350859.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="ONT86_000030"
     CDS             complement(33240..33524)
                     /locus_tag="ONT86_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCR2350860.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="ONT86_000031"
     CDS             complement(33876..34586)
                     /locus_tag="ONT86_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCR2350861.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="ONT86_000032"
     CDS             complement(34583..36055)
                     /locus_tag="ONT86_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR2350862.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="ONT86_000033"
     CDS             36193..36666
                     /locus_tag="ONT86_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2350863.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="ONT86_000034"
     CDS             complement(36819..37370)
                     /locus_tag="ONT86_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2350864.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="ONT86_000035"
     CDS             37475..38005
                     /locus_tag="ONT86_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCR2350865.1"
                     /translation="MKEFKELEDFIGTRFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="ONT86_000036"
     CDS             complement(38488..38931)
                     /locus_tag="ONT86_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2350866.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="ONT86_000037"
     CDS             complement(38947..40107)
                     /locus_tag="ONT86_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2350867.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="ONT86_000038"
     CDS             40614..42314
                     /locus_tag="ONT86_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2350868.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPSFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="ONT86_000039"
     CDS             complement(42416..43249)
                     /locus_tag="ONT86_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR2350869.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="ONT86_000040"
     CDS             complement(43553..45718)
                     /locus_tag="ONT86_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2350870.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="ONT86_000041"
     CDS             45978..48272
                     /locus_tag="ONT86_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR2350871.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="ONT86_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="ONT86_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR2350872.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="ONT86_000043"
     CDS             48923..49930
                     /locus_tag="ONT86_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR2350873.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="ONT86_000044"
     CDS             49952..50971
                     /locus_tag="ONT86_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2350874.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ONT86_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ONT86_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR2350875.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="ONT86_000046"
     CDS             complement(52729..54150)
                     /locus_tag="ONT86_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2350876.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ONT86_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ONT86_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR2350877.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="ONT86_000048"
     CDS             complement(56561..57817)
                     /locus_tag="ONT86_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR2350878.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="ONT86_000049"
     CDS             58397..59698
                     /locus_tag="ONT86_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR2350879.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="ONT86_000050"
     CDS             complement(59767..60366)
                     /locus_tag="ONT86_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2350880.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="ONT86_000051"
     CDS             60620..61501
                     /locus_tag="ONT86_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2350881.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="ONT86_000052"
     CDS             61514..63124
                     /locus_tag="ONT86_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCR2350882.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="ONT86_000053"
     CDS             complement(63201..63860)
                     /locus_tag="ONT86_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR2350883.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="ONT86_000054"
     CDS             complement(64143..64808)
                     /locus_tag="ONT86_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCR2350884.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="ONT86_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="ONT86_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCR2350885.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="ONT86_000056"
     CDS             complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="ONT86_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR2350886.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="ONT86_000057"
     CDS             complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="ONT86_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCR2350887.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="ONT86_000058"
     CDS             67122..67442
                     /locus_tag="ONT86_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR2350888.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68423)
                     /locus_tag="ONT86_000059"
     CDS             complement(67554..68423)
                     /locus_tag="ONT86_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2350889.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(68519..69822)
                     /locus_tag="ONT86_000060"
                     /pseudo
     CDS             complement(68519..69822)
                     /locus_tag="ONT86_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(69819..70328)
                     /locus_tag="ONT86_000061"
     CDS             complement(69819..70328)
                     /locus_tag="ONT86_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2350890.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70503..72638)
                     /locus_tag="ONT86_000062"
     CDS             complement(70503..72638)
                     /locus_tag="ONT86_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2350891.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72721..74376)
                     /locus_tag="ONT86_000063"
     CDS             complement(72721..74376)
                     /locus_tag="ONT86_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2350892.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74378..74692)
                     /locus_tag="ONT86_000064"
     CDS             complement(74378..74692)
                     /locus_tag="ONT86_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR2350893.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74862..75122
                     /locus_tag="ONT86_000065"
     CDS             74862..75122
                     /locus_tag="ONT86_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCR2350894.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75136..75450
                     /locus_tag="ONT86_000066"
     CDS             75136..75450
                     /locus_tag="ONT86_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCR2350895.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76180..76734)
                     /locus_tag="ONT86_000067"
     CDS             complement(76180..76734)
                     /locus_tag="ONT86_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR2350896.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIRLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(76860..78788)
                     /locus_tag="ONT86_000068"
     CDS             complement(76860..78788)
                     /locus_tag="ONT86_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR2350897.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(79094..79810)
                     /locus_tag="ONT86_000069"
     CDS             complement(79094..79810)
                     /locus_tag="ONT86_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR2350898.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="ONT86_000070"
     CDS             complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="ONT86_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCR2350899.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="ONT86_000071"
     CDS             complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="ONT86_000071"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR2350900.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(83280..84740)
                     /locus_tag="ONT86_000072"
     CDS             complement(83280..84740)
                     /locus_tag="ONT86_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2350901.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(84730..86025)
                     /locus_tag="ONT86_000073"
     CDS             complement(84730..86025)
                     /locus_tag="ONT86_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2350902.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(86357..87034)
                     /locus_tag="ONT86_000074"
     CDS             complement(86357..87034)
                     /locus_tag="ONT86_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCR2350903.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87064..88155)
                     /locus_tag="ONT86_000075"
     CDS             complement(87064..88155)
                     /locus_tag="ONT86_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCR2350904.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(88256..88690)
                     /locus_tag="ONT86_000076"
     CDS             complement(88256..88690)
                     /locus_tag="ONT86_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2350905.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="ONT86_000077"
     CDS             complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="ONT86_000077"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR2350906.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(90728..92623)
                     /locus_tag="ONT86_000078"
     CDS             complement(90728..92623)
                     /locus_tag="ONT86_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2350907.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(92681..94456)
                     /locus_tag="ONT86_000079"
     CDS             complement(92681..94456)
                     /locus_tag="ONT86_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR2350908.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(94506..96002)
                     /locus_tag="ONT86_000080"
     CDS             complement(94506..96002)
                     /locus_tag="ONT86_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2350909.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(96196..97818)
                     /locus_tag="ONT86_000081"
     CDS             complement(96196..97818)
                     /locus_tag="ONT86_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR2350910.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(98267..99580)
                     /locus_tag="ONT86_000082"
     CDS             complement(98267..99580)
                     /locus_tag="ONT86_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR2350911.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(99825..100049)
                     /locus_tag="ONT86_000083"
     CDS             complement(99825..100049)
                     /locus_tag="ONT86_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2350912.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100069..100491)
                     /locus_tag="ONT86_000084"
     CDS             complement(100069..100491)
                     /locus_tag="ONT86_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCR2350913.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(100635..101345)
                     /locus_tag="ONT86_000085"
     CDS             complement(100635..101345)
                     /locus_tag="ONT86_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2350914.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(101363..101860)
                     /locus_tag="ONT86_000086"
     CDS             complement(101363..101860)
                     /locus_tag="ONT86_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2350915.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(101897..102349)
                     /locus_tag="ONT86_000087"
     CDS             complement(101897..102349)
                     /locus_tag="ONT86_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2350916.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(102484..103089)
                     /locus_tag="ONT86_000088"
     CDS             complement(102484..103089)
                     /locus_tag="ONT86_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR2350917.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(103539..104159)
                     /locus_tag="ONT86_000089"
     CDS             complement(103539..104159)
                     /locus_tag="ONT86_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2350918.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104161..105030)
                     /locus_tag="ONT86_000090"
     CDS             complement(104161..105030)
                     /locus_tag="ONT86_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCR2350919.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105053..105460)
                     /locus_tag="ONT86_000091"
     CDS             complement(105053..105460)
                     /locus_tag="ONT86_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR2350920.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(105479..106768)
                     /locus_tag="ONT86_000092"
     CDS             complement(105479..106768)
                     /locus_tag="ONT86_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2350921.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(106795..107097)
                     /locus_tag="ONT86_000093"
     CDS             complement(106795..107097)
                     /locus_tag="ONT86_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2350922.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(107122..109215)
                     /locus_tag="ONT86_000094"
     CDS             complement(107122..109215)
                     /locus_tag="ONT86_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2350923.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(109704..110468)
                     /locus_tag="ONT86_000095"
     CDS             complement(109704..110468)
                     /locus_tag="ONT86_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HCR2350924.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="ONT86_000096"
     CDS             complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="ONT86_000096"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HCR2350925.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(111473..112549)
                     /locus_tag="ONT86_000097"
     CDS             complement(111473..112549)
                     /locus_tag="ONT86_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR2350926.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(112573..113166)
                     /locus_tag="ONT86_000098"
     CDS             complement(112573..113166)
                     /locus_tag="ONT86_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HCR2350927.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="ONT86_000099"
     CDS             complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="ONT86_000099"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HCR2350928.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(114836..115135)
                     /locus_tag="ONT86_000100"
     CDS             complement(114836..115135)
                     /locus_tag="ONT86_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HCR2350929.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="ONT86_000101"
     CDS             complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="ONT86_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HCR2350930.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(116890..117801)
                     /locus_tag="ONT86_000102"
     CDS             complement(116890..117801)
                     /locus_tag="ONT86_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR2350931.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            117921..118778
                     /locus_tag="ONT86_000103"
     CDS             117921..118778
                     /locus_tag="ONT86_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2350932.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(118965..119327)
                     /locus_tag="ONT86_000104"
     CDS             complement(118965..119327)
                     /locus_tag="ONT86_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR2350933.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            119673..121112
                     /locus_tag="ONT86_000105"
     CDS             119673..121112
                     /locus_tag="ONT86_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2350934.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(121119..123671)
                     /locus_tag="ONT86_000106"
     CDS             complement(121119..123671)
                     /locus_tag="ONT86_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HCR2350935.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(123785..125116)
                     /locus_tag="ONT86_000107"
     CDS             complement(123785..125116)
                     /locus_tag="ONT86_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2350936.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(125082..126560)
                     /locus_tag="ONT86_000108"
     CDS             complement(125082..126560)
                     /locus_tag="ONT86_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2350937.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="ONT86_000109"
     CDS             complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="ONT86_000109"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR2350938.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            129170..130597
                     /locus_tag="ONT86_000110"
     CDS             129170..130597
                     /locus_tag="ONT86_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2350939.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(130689..131054)
                     /locus_tag="ONT86_000111"
     CDS             complement(130689..131054)
                     /locus_tag="ONT86_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR2350940.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(131051..131416)
                     /locus_tag="ONT86_000112"
     CDS             complement(131051..131416)
                     /locus_tag="ONT86_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR2350941.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(131608..131856)
                     /locus_tag="ONT86_000113"
     CDS             complement(131608..131856)
                     /locus_tag="ONT86_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2350942.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            131855..131983
                     /locus_tag="ONT86_000114"
     CDS             131855..131983
                     /locus_tag="ONT86_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HCR2350943.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            132040..133734
                     /locus_tag="ONT86_000115"
     CDS             132040..133734
                     /locus_tag="ONT86_000115"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCR2350944.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            133738..133857
                     /locus_tag="ONT86_000116"
                     /pseudo
     CDS             133738..133857
                     /locus_tag="ONT86_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            133834..134007
                     /locus_tag="ONT86_000117"
     CDS             133834..134007
                     /locus_tag="ONT86_000117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2350945.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            134385..135518
                     /locus_tag="ONT86_000118"
     CDS             134385..135518
                     /locus_tag="ONT86_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR2350946.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            135549..135869
                     /locus_tag="ONT86_000119"
     CDS             135549..135869
                     /locus_tag="ONT86_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HCR2350947.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(135898..136362)
                     /locus_tag="ONT86_000120"
     CDS             complement(135898..136362)
                     /locus_tag="ONT86_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2350948.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(136494..137747)
                     /locus_tag="ONT86_000121"
     CDS             complement(136494..137747)
                     /locus_tag="ONT86_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2350949.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(137776..138093)
                     /locus_tag="ONT86_000122"
     CDS             complement(137776..138093)
                     /locus_tag="ONT86_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR2350950.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(138119..138439)
                     /locus_tag="ONT86_000123"
     CDS             complement(138119..138439)
                     /locus_tag="ONT86_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2350951.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(138903..140318)
                     /locus_tag="ONT86_000124"
     CDS             complement(138903..140318)
                     /locus_tag="ONT86_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2350952.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(140335..141651)
                     /locus_tag="ONT86_000125"
     CDS             complement(140335..141651)
                     /locus_tag="ONT86_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2350953.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(141801..142499)
                     /locus_tag="ONT86_000126"
     CDS             complement(141801..142499)
                     /locus_tag="ONT86_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR2350954.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(142788..143744)
                     /locus_tag="ONT86_000127"
     CDS             complement(142788..143744)
                     /locus_tag="ONT86_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2350955.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(143857..144690)
                     /gene="msrA"
                     /locus_tag="ONT86_000128"
     CDS             complement(143857..144690)
                     /gene="msrA"
                     /locus_tag="ONT86_000128"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR2350956.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(144865..146499)
                     /locus_tag="ONT86_000129"
     CDS             complement(144865..146499)
                     /locus_tag="ONT86_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2350957.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(146486..148174)
                     /locus_tag="ONT86_000130"
     CDS             complement(146486..148174)
                     /locus_tag="ONT86_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2350958.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        43868 a        30730 c        25744 g        47906 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaataca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcgcttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatctttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa
    70261 ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact
    70321 tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca
    70381 tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca
    70441 tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt
    70501 aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac
    70561 atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga
    70621 tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat
    70681 gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact
    70741 gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca
    70801 gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg
    70861 atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg
    70921 taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata
    70981 atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta
    71041 aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt
    71101 acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga
    71161 cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct
    71221 gagccatatc gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt
    71281 tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct
    71341 acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct
    71401 tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga
    71461 ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga
    71521 tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca
    71581 tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt
    71641 agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc
    71701 gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc
    71761 agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa
    71821 atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta
    71881 tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt
    71941 cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt
    72001 tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact
    72061 tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct
    72121 ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc
    72181 gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa
    72241 tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat
    72301 tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat
    72361 aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg
    72421 atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca
    72481 aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc
    72541 gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc
    72601 agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa
    72661 agtggtggac ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta
    72721 tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc
    72781 atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg
    72841 taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc
    72901 ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc
    72961 tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca
    73021 ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac
    73081 tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa
    73141 gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt
    73201 tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag
    73261 taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac
    73321 ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt
    73381 cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc
    73441 ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag
    73501 tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc
    73561 aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat
    73621 tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc
    73681 agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc
    73741 gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc
    73801 tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc
    73861 taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat
    73921 cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt
    73981 tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt
    74041 tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac
    74101 gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc
    74161 taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact
    74221 gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt
    74281 ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat
    74341 tccacctgct acacctccga caataacgat tttcatctta tttccctcct actgttcgat
    74401 caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt
    74461 ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt
    74521 tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc
    74581 cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt
    74641 cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca
    74701 cctcatttac atttttataa atacctcatg gggtatatta aacaggagct ttattaatat
    74761 gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata aaataccgat
    74821 aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa
    74881 atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg
    74941 aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg
    75001 atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata
    75061 gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt
    75121 aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa
    75181 acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct
    75241 gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg
    75301 caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt
    75361 tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa
    75421 tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc
    75481 ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg
    75541 ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    75601 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    75661 gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc
    75721 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg
    75781 aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat
    75841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    75901 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
    75961 gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
    76021 taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca
    76081 acttctcctg tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt
    76141 tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt
    76201 tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa
    76261 aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac
    76321 atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatc tgatattttt
    76381 agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa
    76441 caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa
    76501 tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc
    76561 aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata
    76621 aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga
    76681 taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc
    76741 gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat
    76801 gatttcactg tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat
    76861 cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact
    76921 agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta
    76981 taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc
    77041 accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc
    77101 gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc
    77161 cgatcacctt ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct
    77221 gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct
    77281 gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt
    77341 tctcctgttt ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    77401 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    77461 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    77521 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    77581 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    77641 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    77701 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    77761 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    77821 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    77881 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    77941 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    78001 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    78061 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    78121 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    78181 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    78241 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    78301 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    78361 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    78421 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    78481 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    78541 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    78601 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    78661 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    78721 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    78781 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    78841 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    78901 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    78961 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    79021 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    79081 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    79141 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    79201 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    79261 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    79321 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    79381 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    79441 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    79501 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    79561 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    79621 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    79681 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    79741 catctccaag caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat
    79801 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    79861 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    79921 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    79981 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    80041 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    80101 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    80161 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    80221 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    80281 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    80341 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    80401 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    80461 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    80521 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    80581 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    80641 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    80701 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    80761 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    80821 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    80881 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    80941 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    81001 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    81061 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    81121 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    81181 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    81241 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    81301 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    81361 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    81421 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    81481 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    81541 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    81601 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    81661 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    81721 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    81781 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    81841 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    81901 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    81961 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    82021 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    82081 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    82141 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    82201 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    82261 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    82321 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    82381 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    82441 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    82501 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    82561 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    82621 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    82681 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    82741 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    82801 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    82861 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    82921 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    82981 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    83041 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    83101 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    83161 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    83221 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    83281 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    83341 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    83401 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    83461 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    83521 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    83581 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    83641 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    83701 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    83761 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    83821 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    83881 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    83941 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    84001 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    84061 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    84121 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    84181 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    84241 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    84301 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    84361 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    84421 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    84481 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    84541 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    84601 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    84661 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    84721 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    84781 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    84841 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    84901 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    84961 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    85021 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    85081 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    85141 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    85201 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    85261 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    85321 gcgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    85381 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    85441 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    85501 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    85561 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    85621 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    85681 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    85741 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    85801 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    85861 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    85921 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    85981 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    86041 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    86101 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    86161 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    86221 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    86281 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    86341 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    86401 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    86461 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    86521 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    86581 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    86641 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    86701 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    86761 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    86821 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    86881 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    86941 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    87001 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    87061 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    87121 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    87181 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    87241 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    87301 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    87361 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    87421 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    87481 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    87541 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    87601 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    87661 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    87721 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    87781 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    87841 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    87901 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    87961 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    88021 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    88081 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    88141 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    88201 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    88261 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    88321 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    88381 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    88441 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    88501 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    88561 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    88621 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    88681 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    88741 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    88801 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    88861 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    88921 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    88981 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    89041 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    89101 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    89161 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    89221 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    89281 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    89341 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    89401 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    89461 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg
    89521 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    89581 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    89641 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    89701 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    89761 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    89821 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    89881 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    89941 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    90001 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    90061 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    90121 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    90181 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    90241 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    90301 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    90361 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    90421 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    90481 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    90541 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    90601 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    90661 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    90721 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    90781 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    90841 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    90901 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    90961 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    91021 ttggaaaaac tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa
    91081 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    91141 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    91201 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    91261 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    91321 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    91381 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    91441 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    91501 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    91561 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    91621 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    91681 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    91741 ttgcataatc tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac
    91801 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    91861 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    91921 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    91981 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    92041 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    92101 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    92161 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    92221 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    92281 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    92341 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    92401 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    92461 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    92521 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    92581 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta
    92641 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    92701 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    92761 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    92821 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    92881 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    92941 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    93001 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    93061 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    93121 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    93181 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    93241 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    93301 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    93361 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    93421 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    93481 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    93541 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    93601 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    93661 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    93721 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    93781 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    93841 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca
    93901 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    93961 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    94021 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    94081 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    94141 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    94201 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    94261 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    94321 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    94381 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    94441 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    94501 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    94561 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    94621 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    94681 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    94741 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    94801 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    94861 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    94921 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    94981 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    95041 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    95101 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    95161 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    95221 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    95281 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    95341 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    95401 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    95461 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    95521 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    95581 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    95641 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    95701 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    95761 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    95821 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    95881 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    95941 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    96001 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    96061 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    96121 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    96181 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    96241 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    96301 tggaacagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    96361 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    96421 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    96481 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    96541 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    96601 cgtcctagca ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa
    96661 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    96721 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    96781 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    96841 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    96901 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    96961 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    97021 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    97081 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    97141 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    97201 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    97261 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    97321 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    97381 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    97441 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    97501 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    97561 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    97621 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    97681 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    97741 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    97801 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    97861 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    97921 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    97981 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    98041 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt
    98101 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    98161 actcagctgt ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg
    98221 tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca
    98281 atatccacat gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt
    98341 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    98401 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    98461 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    98521 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    98581 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    98641 cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca
    98701 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    98761 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    98821 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    98881 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    98941 tcaaacctgt gaccagacct aaaatcgttg tagctgctat cccgatgaag atgccacctg
    99001 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    99061 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    99121 cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca
    99181 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    99241 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    99301 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    99361 aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa
    99421 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    99481 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    99541 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    99601 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    99661 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    99721 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    99781 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    99841 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    99901 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    99961 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
   100021 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
   100081 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
   100141 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
   100201 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
   100261 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
   100321 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
   100381 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
   100441 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
   100501 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
   100561 ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac ggcaatgaat gccgctcttc
   100621 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
   100681 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
   100741 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
   100801 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
   100861 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
   100921 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
   100981 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
   101041 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
   101101 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
   101161 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
   101221 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
   101281 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
   101341 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
   101401 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
   101461 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
   101521 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
   101581 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
   101641 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
   101701 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
   101761 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
   101821 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
   101881 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
   101941 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
   102001 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
   102061 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
   102121 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
   102181 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
   102241 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
   102301 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
   102361 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
   102421 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
   102481 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
   102541 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
   102601 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
   102661 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
   102721 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
   102781 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
   102841 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
   102901 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
   102961 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
   103021 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
   103081 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
   103141 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
   103201 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
   103261 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
   103321 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
   103381 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
   103441 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
   103501 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
   103561 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
   103621 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
   103681 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
   103741 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
   103801 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
   103861 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
   103921 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
   103981 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
   104041 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
   104101 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
   104161 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
   104221 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
   104281 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
   104341 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
   104401 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
   104461 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
   104521 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
   104581 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
   104641 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
   104701 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
   104761 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
   104821 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
   104881 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
   104941 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
   105001 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
   105061 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
   105121 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
   105181 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
   105241 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
   105301 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
   105361 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
   105421 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
   105481 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
   105541 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
   105601 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
   105661 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
   105721 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
   105781 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
   105841 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
   105901 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
   105961 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
   106021 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
   106081 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
   106141 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
   106201 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
   106261 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
   106321 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
   106381 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
   106441 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
   106501 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
   106561 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
   106621 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
   106681 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
   106741 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
   106801 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
   106861 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
   106921 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
   106981 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
   107041 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
   107101 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
   107161 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
   107221 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
   107281 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
   107341 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
   107401 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
   107461 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
   107521 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
   107581 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
   107641 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
   107701 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
   107761 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
   107821 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
   107881 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
   107941 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
   108001 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
   108061 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
   108121 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
   108181 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
   108241 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
   108301 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
   108361 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
   108421 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
   108481 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
   108541 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
   108601 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
   108661 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
   108721 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
   108781 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
   108841 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
   108901 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
   108961 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
   109021 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
   109081 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
   109141 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
   109201 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
   109261 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
   109321 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
   109381 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
   109441 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
   109501 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
   109561 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
   109621 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
   109681 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
   109741 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
   109801 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
   109861 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
   109921 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
   109981 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
   110041 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
   110101 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
   110161 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
   110221 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
   110281 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
   110341 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
   110401 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
   110461 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
   110521 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
   110581 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
   110641 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
   110701 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
   110761 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
   110821 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
   110881 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
   110941 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
   111001 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
   111061 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
   111121 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
   111181 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
   111241 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
   111301 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
   111361 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
   111421 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
   111481 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
   111541 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
   111601 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
   111661 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
   111721 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
   111781 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
   111841 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
   111901 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
   111961 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
   112021 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
   112081 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
   112141 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
   112201 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
   112261 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
   112321 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
   112381 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
   112441 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
   112501 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
   112561 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
   112621 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
   112681 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
   112741 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
   112801 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
   112861 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
   112921 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
   112981 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
   113041 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
   113101 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
   113161 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
   113221 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
   113281 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
   113341 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
   113401 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
   113461 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
   113521 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
   113581 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
   113641 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
   113701 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
   113761 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
   113821 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
   113881 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
   113941 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
   114001 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
   114061 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
   114121 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
   114181 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
   114241 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
   114301 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
   114361 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
   114421 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
   114481 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
   114541 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
   114601 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
   114661 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
   114721 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc
   114781 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
   114841 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
   114901 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
   114961 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
   115021 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
   115081 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
   115141 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
   115201 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
   115261 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
   115321 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
   115381 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
   115441 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
   115501 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
   115561 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
   115621 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
   115681 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
   115741 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
   115801 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
   115861 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
   115921 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
   115981 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
   116041 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
   116101 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
   116161 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
   116221 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
   116281 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
   116341 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
   116401 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
   116461 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
   116521 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
   116581 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
   116641 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
   116701 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
   116761 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
   116821 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
   116881 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
   116941 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
   117001 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
   117061 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
   117121 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
   117181 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
   117241 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
   117301 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
   117361 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
   117421 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
   117481 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
   117541 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
   117601 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
   117661 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
   117721 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
   117781 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
   117841 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
   117901 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
   117961 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
   118021 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
   118081 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
   118141 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
   118201 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
   118261 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
   118321 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
   118381 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
   118441 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
   118501 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
   118561 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
   118621 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
   118681 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
   118741 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
   118801 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
   118861 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
   118921 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
   118981 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
   119041 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
   119101 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
   119161 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
   119221 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
   119281 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
   119341 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
   119401 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
   119461 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt
   119521 ttttgatttt agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg
   119581 ctccagaaaa ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct
   119641 ccgtattctt agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta
   119701 ccgaaaaaga tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga
   119761 taaccgtaaa tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc
   119821 ttcaatctat tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa
   119881 tcagtttgca aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac
   119941 aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg
   120001 attttctagc cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca
   120061 atcagcgttt agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc
   120121 ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata
   120181 acaactattt tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa
   120241 acctcagtca aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct
   120301 ttatcaatac gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca
   120361 gcacactctc ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt
   120421 atcttaaaga cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca
   120481 acaataacta tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc
   120541 tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat
   120601 ctctgactga taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg
   120661 aagcattgga ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact
   120721 tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac
   120781 caaattactt tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac
   120841 cgaatttgtt caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc
   120901 agcaggaatt aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg
   120961 atctttccac tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt
   121021 ctctagatat ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt
   121081 tgactgaact cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct
   121141 gttttttctt ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag
   121201 aatagaagaa tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta
   121261 ccgatcagct gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata
   121321 cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac
   121381 aactctctgc aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg
   121441 gtttgctgta agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc
   121501 agtagggaga catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga
   121561 tctcttggtg ggcgcttcat gatttctgga tctggcgact cgaaccctag tgcataagat
   121621 aaagtgattg tcgttaccat attgacccat aaaatctgta ctggggacaa aggtagcggg
   121681 cgattcagca taagtgccca aatcaagatc aatccttgag ctaatgcagt tggcaagaag
   121741 aaattgatgg ttttttgcaa attatccaaa atccgccggc cctcttttac ggcttttgca
   121801 atcgtatgga aattatcatc tcctaaaacc atatcagctg cctgtttgct tacttcacta
   121861 cctttgattc ccatagcaat tcctacatcg gatcttttca aagcaggtgc atcatttacg
   121921 ccgtctccgg tcattccgac gatttctcca tttttctgca aagctttaac aattcgcagt
   121981 ttatgttctg gtgtcgttcg agcaaagaca tcgactattg gcacttgctg aatcaactct
   122041 tcatcagaca taagatccaa atcgatccct tctaaaactt tggcagtatg ttttaagcca
   122101 atttgttccg caatcgcttt tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag
   122161 atccctgctt gttggcattc ctttacagct ttgactgcac tttcttttgg cggatcgatc
   122221 aatccggcga tccctgcaaa agttaattgg gttaaagtct catgcgtgag ttcctgtttc
   122281 gaatctactt ttttataagc aaatcctaaa acacgctgac cttttctcgc aaattctgca
   122341 gctgtctgac tccatgcttc ttgttctgca gaagaaagag ttgatttagc aaataatact
   122401 tctggtgcac ctttgacgta aatcaccgct cctttttcct ccgcatgcat cgttgccata
   122461 tatttgtatt cagaactgaa cggaatcttt gcttctattt ttttcaaagg tagattggct
   122521 gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc
   122581 tgttcttctt ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct
   122641 tcttttgcag ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct
   122701 gaacagatca ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc
   122761 ttttcttttg ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat
   122821 ccttcgggta ccattgacac caccaaagca atcgtagccg aaagaaggat atgccaatcc
   122881 ataccatatc gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga
   122941 aaaatttgtt tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc
   123001 gattgcaatg cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca
   123061 ccgactgcag aaccagattg tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg
   123121 atcgattctt cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc
   123181 aaaatcgatt cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg
   123241 acatctccgg cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt
   123301 tcttgcatcc cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc
   123361 aatgattctt ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg
   123421 aataaaataa tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg
   123481 atcaacacga tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc
   123541 tgctgccttg cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct
   123601 tgcgacaagc cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg
   123661 taccagttca tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt
   123721 tttcaggcgt atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct
   123781 gggcctaact gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc
   123841 agataaccaa taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa
   123901 ttgaagtaaa aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct
   123961 tttctttaat aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac
   124021 tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta
   124081 gacaaagacc ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt
   124141 tcttagaaag cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa
   124201 tcctgctagt actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta
   124261 gattgacaat tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac
   124321 tttcttttaa atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta
   124381 tatagaaaaa gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg
   124441 gatcacttgt ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa
   124501 ccataggaaa tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc
   124561 caaaattttc tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac
   124621 ccaataagat aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt
   124681 caccaaacca cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc
   124741 cgcttatcaa agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta
   124801 attccgcaag tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc
   124861 actttttgcc taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta
   124921 aaaataaatc acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa
   124981 cgagaataac agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg
   125041 ttccaattgc gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca
   125101 ttgccggttt ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt
   125161 tcccaagaag gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg
   125221 atggctgtga ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa
   125281 aatccgattg ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc
   125341 tctttttttc caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa
   125401 actgcgatcc ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc
   125461 atcaaaaatg cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata
   125521 atgacagact gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa
   125581 ttgaattttg gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct
   125641 gaggcccaag cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc
   125701 acaataagat gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta
   125761 ttcaattcca tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg
   125821 acgaaaaata taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt
   125881 tccaatcgag tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc
   125941 gcaaaataaa caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa
   126001 ctgcctaatg ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag
   126061 agcatcatcg tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt
   126121 gccatatcaa atttcaaatt caaaagagtg atgatccaat aaacgactaa aatgacaact
   126181 aatgtaaatt tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga
   126241 gcgagtgctg aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag
   126301 acaacgaacc cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt
   126361 tggggaaaca ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat
   126421 actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt
   126481 cgtacacttg ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt
   126541 gatgaaccag tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt
   126601 cttttatttt tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag
   126661 tatcaccgaa acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg
   126721 atccaactta tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca
   126781 gggggaaata caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt
   126841 tattctttca cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc
   126901 tttggcgatt ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat
   126961 gatagccgca cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc
   127021 cattctttca acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg
   127081 agaagtaata aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat
   127141 tttttcattt aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca
   127201 attgaccata ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac
   127261 agtaaaagtc agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc
   127321 aatcaattga ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc
   127381 ggcggcggtt gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc
   127441 ctttactgat ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt
   127501 atgtttgatt acgattcctc cagcagcata agggacatag cccatcttat gaggatctat
   127561 cgtgacagaa tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt
   127621 tacgcagtgg tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac
   127681 aaattctcct gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac
   127741 atgtaaatag aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc
   127801 tacactgtct acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg
   127861 gattttttgt ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata
   127921 atcctcttga actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc
   127981 cttcatccaa gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt
   128041 ttttccgcta cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc
   128101 ggtcatttcc aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt
   128161 cactttttca ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta
   128221 ccataatcct tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt
   128281 catattgaat aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat
   128341 agaagattcc aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa
   128401 agcaggcatc aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg
   128461 gatcgatcct gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg
   128521 ggtggctaga taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg
   128581 aatataattt tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata
   128641 aactggtcca ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat
   128701 ttttcttccc tccatatttt gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac
   128761 acctcttcac tatagcgcca aaaatgagaa attttttttt ataacatcaa atccacgtag
   128821 atttttacaa acaaaaataa ataaagtcgg atttttctga taattcaacg caaaaaagcg
   128881 aacaattttt caataaatca gtcttctaaa atagataacc taaaaaaagc gaacaaacaa
   128941 ataaaatgac tgtccttcat tcactaaaca tatttttatt tttttatatt gttaaaaatt
   129001 cacaatttat tttttaatag aattatctgt tttccaaatt ttataacata aaaaaccttt
   129061 tttaacggca tttatatgct ttttcagaaa ttaactgatg atttcaaaaa gaaaacatgt
   129121 cataatattt cccatattgt gcaaaaaaat acaaggcgga ggctctacta tgattgaaga
   129181 atatattgag aaaaatattt taagacagct gtttttatgt gggcaattct atgtaaataa
   129241 agaagtcaat ctagaaaagc tctccaatct cctgcatgtt tgcaaaacca ctttactcaa
   129301 tgatatcaat aacataaaaa aagaatttga agaacagatc gcttatacac atcgggaaaa
   129361 ggaccgttac actttatatt tttcagaaca tatacctcga tgcaagatca tgcagcaatt
   129421 gtctcaaaat tcgttgtttt taaaaacttg tctgctttat ttggaagaag atgaacctga
   129481 ttatcttcaa ttaactgaat gtgaattcat ctctgtcagc aaagcttatt cattaaaaaa
   129541 acaagtatta gcttacttta atgactgcgg gatcgaaatc gaccgctact cccctcgatt
   129601 cactgaaatg gaaagacgtc tgctgcttct taatgtgtcg tatcgtttag gcggttttaa
   129661 ttcttgggag ctacctgaat cgttttttga acgtgccgat cgtttcattg aatccgtaac
   129721 ggaaaatagt ggcagatttt atgataaaga aaataaagaa atcttatcga ttggttttgc
   129781 tatcagtttc ctacgccagc aagtgtgtgc tgtgacaata gattcaaaat ttatcgaaga
   129841 aatcaaaaaa cgtcctattt acaattatgt cgaatcagca tgggaaaaca ctgatttcca
   129901 aacctattat aaaaaagaag aattcgcctt tattttgaca ctattcaatc tttgtaatta
   129961 tggtttccat tcttatcagt tgattgcgga agactttcaa cagctacacc aagtgtttat
   130021 tgacaatact cccgaaataa aagaactagt tgctaccttt gaatctcact tcaatcaaga
   130081 attattcgga aaccaaccgt tcgaacgtgc gctgattcat ctgatgcgtt cggcttggga
   130141 taactatcaa ttattcatgc cagaaaaatt ttatctttta aatgaggaac agacaaattt
   130201 atataaagaa gtccagacaa ttttttccag ctggtccagc cagttacctt atgatctacg
   130261 gttgaatcct aattgcatgc gggcttttgt gatcgaattg agcggaattc tccgtctgac
   130321 aaaagaacat ctgactatct atatcgtcac aaattcggat gttcattatc tgatttatcg
   130381 ggaagccttg gaagctgtaa ctactttcga ttttcaagtt gcaccaacaa tttattcaag
   130441 tatatctgat ataaagaagt atgcgcagca gtcctcaaac cgggtattat gtgaacgtac
   130501 attatataca ccagatgcgg tccaatatga aaatattatt cctatttcga tcaatacgat
   130561 cgatcgagct atcatttcag ctgtgcaaaa caaataaggg gctgtgacaa aaatcatgtc
   130621 acagcctctt atttgttgaa gatgcccatt tctgtaacaa tctcttgata cacgtcaggc
   130681 tcacaacctc acttattttg gacatctagg ggtccgatct ctttatttaa aaaataagaa
   130741 atgatggttc ggataactac caacgccgct agttggaaaa tatcctgcaa ggtaggtttg
   130801 ataatcgatt cgatgatccc agctgaaaca agaatttcca aacttagaag tatatagcct
   130861 cccaacatct ttttaatagc attgttctcg cttactcggt cgctttgcac ttttgtatgg
   130921 acaaataaat atttcaaaaa atccttgatt gatagacaaa caccccaaac caataaacag
   130981 atagcaaaga tatcaagtcc cagtacaagt ttttcgaaat atggaagaag aaactgcatc
   131041 aactctgtcg tcatcctacc tctcctcttc tttgtttttc tttgtcttct atttcctgat
   131101 tcaagaaata agaaatgact gttcgaatga taataataag tgcaagcttc aaaatatctg
   131161 tccaagtcgg cttgataact gactcgatga tatcagcaac gactagtact tccaagctta
   131221 acaaaatata acttgctagc agttttcttg tatctgcatt acgagtatta cgttctgcat
   131281 agtttttctt gacatgagac caatcaaaaa gtttgaatag aacaagaacg atcccgacaa
   131341 ttaagatacc gatagaacaa atatttaaaa tagcaatgat ccattctaga aattcaatta
   131401 aatttgagcg aaacatatac gctacctact ttcttttaat ttgttcctat tcttttattg
   131461 taaaagaaag ttaccttgtt cgtctattta aaagttgttc taatgataac ggttcgctta
   131521 aatttttctt tttcactctc ccatttttct ccctccatcc ataaatcgtg gaagtatcca
   131581 aaacaacgta gcaagttaaa ataaggctta gtccgtcatc agcttttcaa agaagcactg
   131641 tctcggtgct ttttttgtac ccaaatttct aaaaatagaa ccatacacct cctcaaatct
   131701 tttcttctta cctaccctat ttttatttac aggaaaatca gacacttctt cttttttgtc
   131761 aaaatttcct ttttcttctc gtcctatcat ttggcattct aataaaaacg aactatcctt
   131821 ggatatagaa agagaaaaca aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg
   131881 catcggttta ggtttgcttg cccttggcgc tgcatatcta ggaaaaaaga caggactatt
   131941 cgaagatgac agtcatttat atgatgaatt cgaatcaatc tagcatccta tttagttgaa
   132001 tcactacgga catacttgga agacattgga ggaatcacaa tgagaaaaag taaagcaata
   132061 atgattggtg ccggtattgc caatatggca gcggctgttt atttgattca agaaggcaaa
   132121 tggaaagggg atcagatcac cttttattca ttggatgatc acggttcaaa tgatggtgca
   132181 ccgaccgaca ctgtagtgga tgaatattgg aataaaaacc atccattaga aaaccaaaaa
   132241 ggctatattg ctcgaggcgg tcgcatgctt aattaccgta cctatgtaga tttgatggat
   132301 ctgttaagtc gaataccttc tgttactgag ccagggatga ctgctgaaga agatacgaga
   132361 gattttgatg caaaacaccg aacctttgac aaagcacgtt tgatggaagg tggcaaagga
   132421 atcattgatg gcggaaaact gggattcaat aataaagacc gtattcttct gactaaactg
   132481 attgctatgc ctgattcaga agaagaaaaa ctagacaata tcactatcga agaatatttc
   132541 aaagatgatc cacacttttt tgaaacaaac ttctggttta tgtgggaaac aacatttgct
   132601 ttcagaacac gtagttctgc tcaagaattg cgtcgctata tgcatcaaat gatttatgaa
   132661 ttcactcaaa tcgaacatct agtgggcgtc aatcgaacac gttacaatca atacgaaagt
   132721 attatgcttc ctttgatcga gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc
   132781 cgtgtcactg actgggaatt caaagaaaca ccgatgcaag atgaaatcac tgtgactggt
   132841 ttgaaaatta ccaatacact gactcaagaa gaagaatacg ttgtggtcga tgaagataca
   132901 gcagttattt tcactaatgg gtccattaca gattcagcta ctttaggaga ttacgaaaca
   132961 ccagctgtag aaaatatgga ttatggtgca gcttctagtc tctggaaaaa agccagtgaa
   133021 cgtttctaca acttgggaaa tcctgacaaa ttctttgctg atcgagatgc aagcgaatgg
   133081 gtcagcttta ccctgacgac gaaaaaccat ttgttgttga atgaaatcac ccgtatcacg
   133141 actcaagaac caggaaatgc cttgaattca tttatttcaa ccacaccgat cacgccgtta
   133201 ggccaaaaag atgtcaatat gtctattgtc gtgcatcatc aaccgcattt tacgtctcag
   133261 aaaccaaatg aaacagttat ttggggttat ttcctttatc caagacgacg tggcgaattt
   133321 gtggataaac agtatattaa aatgaatgga aaagaaatgc tagaagaact gattggacaa
   133381 ctttcaaaag tagatcctgg accagtgaat attcgtgaaa aagaaacaga aattttcgac
   133441 agcgtaataa ataatatccc tgtctatatg ccttatgctt ccgcgttgtt caataatcgt
   133501 gccaaaagcg atcgaccaaa agtcattcct aaacattcga ccaatctggc ctttacagga
   133561 gaatttgtcg agcagcctta tcagatgatc tttactgaac aaagtgctgt acgttctgga
   133621 gaaatcgctg ctttccattt tgcgggtatt cctatgtccc gtcttgtcaa aacaccgcgt
   133681 tacgacaaag atattcctac tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga
   133741 ggttgtgaaa aagctgccct gcatcaagta ataagaacaa ccaggctaaa acagcttctt
   133801 atgtttttga tttggttgga cttattaccg cagatgcaag cttttgaaca ccgtttacca
   133861 agaggttatg aaaaagctgc cctgcatcaa gtaataagag cagccaagct aaaacagctt
   133921 cttatgtttt tgacttggtt agacttatta ccgcagatgc aagcttttga acaccgttta
   133981 ccaagaggtt atgaaaaagg cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg
   134041 ttcctcacgt ttttgcataa atttgactta ataccgagaa actgatccct tctgtcataa
   134101 cctcttttta tttatgtctg tttctttttg aaaacaacta ttttttcaac gtttactctc
   134161 ttgccaatcc aacatcatct aacggataca actatttcaa tgattaccat tacactaaaa
   134221 aatcggaaac acacctctcc gaacacaaat acataagact atagaatgtt tcatttaaga
   134281 aaaaaagcgt ttcattatca aaaaggtaag atctccttta aaatgaaatt atctgtagta
   134341 ctaataatgc agacaatttt atgaatctga aaggagcatc tccaatgaaa gcagtcactt
   134401 ggcaaggaaa acagaaaatg gaaatcagaa acgtagatga tccaaaaata ttagaaccaa
   134461 cagatgcaat catccgtatc acagcaacag ccatttgcgg ctccgatctg catctttacc
   134521 atcatggtga agcagttttg gaaaaagggt atgttgtagg acacgaacca atgggaatcg
   134581 ttgaagaagt gggatctcaa gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt
   134641 ttaatattag ttgtggtcat tgtcatttct gcctgaatca catggaaagc caatgtgatg
   134701 aatcaaatcc agatcagtta tatggcgggc ttttcggatt tggcaagttg aacggtaatc
   134761 attggggtgg tcaagcggaa tatctaagag ttccttatgc agactctact tcatttgttg
   134821 taccagataa tgatcttcct gatgaaaaag ttttatttct atcagatatt ttgcccacag
   134881 cttattggag cgttgaaaac gcaggagtaa aaaaaggcga tacagttgtg attctaggtt
   134941 ctggtccagt cggtctattc gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa
   135001 tcgcagtaga tccagtacag caccgtctag ataaagcggc taattacaat ggggttgaaa
   135061 cttatttctt agaagacacg gcacaagctg gaacagaatt gtatgaactg gctaaaggtg
   135121 gcgcagatgt cattatcgat tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag
   135181 cgaaaaattt agtaagctta caaagcggca caatctctcc attacagatg gcatcgcaag
   135241 ctgtcaaaaa atttggaaca gtccaagtaa ctggtgttta tatgactcca gcttcttctt
   135301 acccgctgca agaatttttt atgcgggata tcgctgtcaa gcatggacag gcaccaatta
   135361 tccatctaat gccaaaaatt tacgcaatga ttgcagaagg actattcgat cctacacaaa
   135421 tcattactca ttcgctgcca ctatcagaag cagcacaagc atatgagatc tttgacaaaa
   135481 aagaagataa aaatataaaa gttgttttga aaccttgaat tcagtaaaaa atacaggtga
   135541 ttttctttat gcccaaaatc aaacgaaata cccatgagat cgaaatagga aaagaagaag
   135601 atatcgaaat acgaggacgt cgcttccgct tctatttcca aaatcgttac acattgatct
   135661 cactggctgt cgatttatta accgggattt tctatatttt tggaagcctt gcttccttaa
   135721 ctaaaatccc cgatcgatat ggcacctatt tttatttggc tggtgctatt ttcttgacga
   135781 ttcgtcctat tctcagaatc gtaaggaaca tttttattta cgacgaaaaa aaagcaatga
   135841 agagaacaag caaaaaaaaa gaggcatgag aatttttcaa atctcatgcc tcttttttca
   135901 ccctatcgta tcactttgat acagacgagc ggtcgtccca ctttcttcct tgaccattgt
   135961 caaaaatgtc cactcgtatc gttcataaaa cgtggtatga tctgtaatta agtaaagttt
   136021 ttcgtaccca agttgatgca tttcttttct gatttccgct aatatccgct tggctatccc
   136081 ctgcttacgc ttttccggtt caacaaacag ggcacaaaga tttggtgtca gatcagggcg
   136141 gttatggaag tcgttttcaa tgacgcccgc tcctccgacg atggtttctt tttcatctaa
   136201 gaccacatac cattgaggaa tactttcctt attagcgatt ccagtacgaa tactttcctc
   136261 atacacaaca gcaggaactc cccattttga tgaaaaccaa ttcgcgcttt gttttactaa
   136321 ttctgggtgt tctcttaatt tgatccagtt atatgtaacc aatgtttaaa cctccgacta
   136381 aaataaaacg tttacaaaaa ttatacaagt ctttatgaaa attagcaatg acaacaatag
   136441 tgatgtaaaa aaaaaaacgg aaaacaatca cttgttctcc gttttttgat taatcaagca
   136501 tctaaagctt cttttcttct ttcttggatc gtagctacct tgacgaacgg tagatagatc
   136561 aaaatagaaa gaaccaaatt gacagcggct agtacaccac ctgcaatact ttttgttgcc
   136621 aatacaccgc caataattgg tggagttacc catggaggca taacggtagc tgctggcact
   136681 aaacctgtag aagtcgcaaa gtaagcaaca ctaactaaga ccattggtgt caaaataaat
   136741 ggaatgaaca tgatcgggtt caacacgata ggtaacccaa acatcgttgg ttcattaata
   136801 ttgaagacac ctggcgcaat cgacaagttt gttacaacca tatatggttt gtttttacgt
   136861 cctactaaat aaatagctaa cagtaaacct aaagttgcac ctgttcctcc taagttgaca
   136921 aagctgtcaa agaaaggttt gttcacgata aacggtgcgc tatgtccagc tgcgattgca
   136981 ttaacgtttg cttcaatggc tggcgcattg attgtttgca tcaaaggatc gaccatattc
   137041 gctccgtgta aaccaaagaa ccataagaat ggtgtgataa atgcaagcag taaggcagat
   137101 ggataagagt ttgcaagtcc cataaatggt tcttggacca ctgtatagaa agaacctacg
   137161 atatcatcaa caccgaatgc agcaaaaatt gcagcaacta aagcaaacgc agagatagtg
   137221 atcattgctg gtagtaatga agcaaatgct tttgcaactg ccggtggcac accatcaggc
   137281 attttgatga tcaatttttc atttccgctt aaacgtacaa agatttctgt tgaaataatc
   137341 ccaacaataa tagcaataaa caaaccgttt gagctcatac ctaatgctcc gcctaaagcg
   137401 aagaaagagc ctaaagaaat cacacctgcg gaaacaccgt cttttcggta gccgttagct
   137461 aaatgataag caagcaagaa agcaatcaag acagaaagaa tggcaaatgt accgttccaa
   137521 gcagcagccc cgaatccttt ccatgattca ccaccaaaaa ttgagttcat caattcttgg
   137581 aacccgggaa tcggtaagtt attgatcatt actgctaatg ctcctaagat cattaacggc
   137641 atactcacca taaatgagtc ccgaatcgca actaaatgcc gttgtgcacc aatttttgat
   137701 gctacaggta taaaatgttt ctccataaat gcaacaaatt tatccataaa tcttccctct
   137761 tttctttact gtagattacg cgttcaatcg acgatacaga ttaatgatct cttttgctaa
   137821 atctgtaaaa gctatagatg tcatcagatg atcttgactg tggaccgtca aaagcgtgac
   137881 ttcaacgtga tttccttgtg cttcttgtgt cagcatctct gtttgagagt gatgtgcttg
   137941 gactaaagat tcctctgcat ctttgatttt ttcatctgct aaagtgaaat cacctttttt
   138001 cgcagcagca atcgcttcca ttgcatcgct ttttgcattt ccgccaaaca tgatcagacc
   138061 catgatggct tctagatttt tttgatcttc catttattgt atcctccgat aatttttatt
   138121 atttgtttat tagtttttct gcttggtcga gtactttttc accattcatc atgccatagt
   138181 cagccatatt gattacatct aaaggaatgc ctttaggtgc tagcttttca gcaaattgat
   138241 tttgcataaa gcgtacttgc ggtcctaaaa gtaaaacgtc tacattttta gattctagtt
   138301 gattatccgc atctgaagca gatacagcaa agatgtctgc ttccattcct tttgcttctg
   138361 ctgccttttg catttttgtg actaggagac ttgtactcat tccagctgaa catacaagca
   138421 tgattgtttt cttttccatt ttaaacacgt tcctttctat acttacactt gtatataagc
   138481 aagtagcgtg ccaactttaa aagcgcttta aaaagttgat atcatcacat ttaaggtcta
   138541 taccaagttg catacacact aaaaaagaaa gcgcacacta aacacacaaa ttaattttta
   138601 ttaccttttt tcttttaacg tgcacaaaaa aagttgtgac acaaaacttg tcacaacttt
   138661 tttcaaataa acggtattca aaagcttgca tctgcggtaa taagtccaac caagttaaaa
   138721 acataagatg ttgtttttac tttgctattc ttattacttg atgcaggaca gctttttcac
   138781 aacctctcaa taaacggtgt tcaatcagct gaccctgggt attagctcaa aacgctggat
   138841 tcacaacctc ttttatttca aataaccagt aaatacaatg aacatctaca aaaaacttct
   138901 atttatttat agatttcgtc aggcaaatcc aattctctct tatcaatcaa agtctgatac
   138961 cacgaagcag attttttcaa tgttctgctt cgttcatcgt tcaattcgat tcgaactaat
   139021 ccatatcgat tcttaaatgc attcattggt gacacacagt cagtgaatgc ccataacata
   139081 tagccttcgc aatttgcgcc ttcttctgtt gctttgatca agtaatgcaa atgctgtccg
   139141 ataaattcga tacggtaatc atcttgcacg attcctttct catccatata ctgttcttca
   139201 tattctcttc ccataccgtt ttctgttatc agccaaggga tattatgata attttctttt
   139261 agatacatgg ctatatcata catgattttc ggatagattt cccatcccct ggaattattc
   139321 attttcttcc caggtaaatc aaagacttca taatatcgtt caggatggaa aggtgtctct
   139381 tcattccatt gataacgagg ggcgcgaacg cgtttaggat aatattgatt gattccaagg
   139441 aaatctactg tatgatgctg aatcgtttct aaatcctctg ttgcaggatc gaaataaatc
   139501 gattgttttt gacacaactc caggaactct tctgtgtagt atccttttac catcggatca
   139561 aaaaatactc gattataaaa caaatcatac atataagcag ctttttggtc ttctggagaa
   139621 ttggatcgag catagaccat ttcaggattt aaaatacagc caatccttcc accataattt
   139681 ccgctgcggt aaatctctac tgctttagcc gttgctaaaa ctttatgata attccacatc
   139741 atccactttt ttgtgttttg ttcatgcggc cagcgaacag catccaaata gcaacgcgtt
   139801 tggggaacga ttggttcgtt gaatgtaaac cagtttttta ccctatcacc gtaacgatca
   139861 aaggcaattt gagcatactt agcataaaaa tcaactactt ttcttgaaga ccagccatca
   139921 tatttttcca aaagaaaagc tgggacttca taatgttcca aacaaatcat cggttctacg
   139981 tctgcttcga tcaaagagtc gatcatttca cttatatgct gagcatattc ttcatctact
   140041 atacagttct cataatcagt aaaaaaacga gcccaattga tagaagtccg ataatgtgtc
   140101 agatggatgg atttcatcag atccacatcc tgctggtatt gttccataaa attagttgct
   140161 acggctgggc catacccatt atgccaaaca aaatgttcat ttttatacca gctatctata
   140221 aacgaatcct gtccttcttt ttttcctttc caaccttccg tttgccaagc agaagatgca
   140281 gcaccaagaa taaaattttt tggaatagta attttcatga tttcctcctg ttttttagat
   140341 cccatcattt tccacacgat ttgctacttt tacaaatggc agatagatca aaatagaaac
   140401 aataatacaa acaagttgcg taataactgc accgatattt cctgctgttg ccagataagc
   140461 attgacgatc ggcggcattg gccaagggac cattacaact gctttcgccg caaatcctaa
   140521 agcggttgca aaataaccaa tcgttccagt gataagaggc gttaagataa acggaatagc
   140581 taaaattgga ttcagcatga tcggcatacc aaaaataact ggttcattga tgttgaagat
   140641 cccaggagca agggataact tggtgatttg tttcatatct tcccgttttc caactaacaa
   140701 tactgcaatc agtaaaccga tcgtgactcc agacccgccc atactcatat acatatccca
   140761 aaatggcata gtaataatgt tcggtacttc ttttccagct tcaaaagctt ccgtatttac
   140821 agcaattgcc gctaaaagca acggttcccg tatcggcttc accatttgat ttccatggat
   140881 accaatcacc caaaagactt gcgcaataaa catcaatagc aaaatacccg gtaacccttg
   140941 tacgacacct tctaatgggc gttgtacgac attgtaaatg atatcataaa ggtacattcc
   141001 tgtaagttct ttgatgataa atcccgcagc cgcgataccc gatacagtca gaatcgtcgg
   141061 gaataaagca gaaaaactag cagaaacatt cgttggcacg gtatcaggca ttctcagctg
   141121 tagtttcttc tgtttcctca accagcaata aagttcaata gaaaggatgg agaccaacat
   141181 tcccaaaaat aaccctttcg tatctgtata ttgtttagcc aatacgtttt ctactataac
   141241 agtctgattt tcattcacta attcataaac agttggatta acagataaat aagacatcac
   141301 tgcgatgata ccagggaata caccatccac tttattcagc ttggctaatt cgatccctat
   141361 cagaaaaaca gcatagatgg tcaagaaact tagtgtgaca tagctgatgg cagatgcgat
   141421 aggctttaat tcttctaaaa aacgcaagaa acttatctgt gctaagccat tttccgagtc
   141481 aaagaccatt gctgaaaaca atgtgccaaa agcacccgta ataattaccg gaatcaatgc
   141541 agcaaatgcg ttttttatca ccatgatata ccttaaatta ttgattctcg ttgccacgtt
   141601 ccctaaaaca ttggctaatt tatccataaa actcattcca gatcccctca ttcctgcttc
   141661 ttctccttga cgtcatctct ttttgtctat tagtcagcgc ttacatttcg atgaacagaa
   141721 actatttact cctctttttt accaaatata aaacaaaaat tcatctgatg tatttatcat
   141781 aaaatacatc agatgaactg tcaattagtt tctttttctc ctattatttt ctgtaattct
   141841 ctccgattta ttgccatatg gacagtcatt gcatcaaacg cagcaagtgc attccgcctt
   141901 tcgatggcct gaacgatctc tgtatgaata tcgatcgtct cgattttcag atgctggata
   141961 tcataatttt tattaaacaa agcaattgat tcattaatga ttgggataat atgcagcaag
   142021 gctacgtttc cgctagcttc tgcaatcatc gtatgaaaat cgatgtctaa ttgaacatgt
   142081 tcttgtgtgc cttctcgaaa agaagtttca atcttttgcg ttaatttttt catttggacg
   142141 atttgattct tcgttgcata ttccgctgcc aaacctgcca tcttaggctc caataaatat
   142201 ctcagttcaa ataagtctcg tatcatccga tcctgatctt caatcaaaga aaaaccgagt
   142261 gggtcattgg caatccctcg cttatcactg acaaatgtcc ccgctccttg ccgaacttca
   142321 aggatattcc tggaagctag agcccgcaca gcttctcgca ccgtgcttcg ccccacctct
   142381 aaataatcgg acaattcata ttcattcggc agtttatctc ctacttgcaa ctgcatttcc
   142441 tttaaatatc taaggatctt gtcagtagtt acttctacta gggttttatt ttcttccaaa
   142501 ccacatacct ccttttcatc tgatgtattt tttcgtaatt attataacac gtttgaatca
   142561 tctgatgatt ttttgtatac aaaattgatt aatccgttca tagacataca aacgaagaag
   142621 atatttacca taaaaaagac taggataaaa ttcactccta gccttcgatc atttttattt
   142681 tgaacgacaa aaaatatgat gttcaataac agacgatctt cattcgattc ttgatgtcat
   142741 cttttgtttt ttttaatata tggataaaaa gtttgattac atcgtttcta ttctagaatt
   142801 tggtcgattc tcaattcttc ttctggtgag aaatctaaat tatccatcat tcgaacactt
   142861 tcttgtaact gacttaaccg acttgctccg accagtacac ttgtaacaga tttttgtcgc
   142921 aaattccatg caagggccat ttgcgctaat gattgtcctc gtgatacggc gattgtttgc
   142981 agtgctttga ttttttccag agttgatcca acttgttctt ctgaaaggaa tggtatttcc
   143041 ttacgatgtg ctctagaatc ttcaggaata ccatgtagat aacgatttgt cagcaaccct
   143101 tgagcaagcg gactgaacgt gattgcccct agcccttctt cttctaatac tggagataga
   143161 tcgtcttcta gtagtgcacg agaaaacata ttgtagcgca tctgatgaat aatgaacggt
   143221 gcttcctttc tttttaaaat ttcagtcatt ttttttgtat cttctccatt atagttggat
   143281 ataccaatat ataatgcttt tccttgtctt acaagttgca tcaaagcttc tgctgtttct
   143341 tccaaaggtg tatcagggtc tgggcgatga tgatagaaga tgtcaacata atctagcccc
   143401 attctttgga gactttggtc acaacttgaa atgatggatt tacgagagcc ccattcccca
   143461 tatggccctt cccacatatg ataacctgct ttgctagaaa taatcagttc atcacggtag
   143521 gcttgaaaat cttctctaaa aatacgccca aagtttttct ctgcagctcc agcagggcct
   143581 ccataattat tcgcaaggtc gaaatgggta attcccatgt cgaatgcgcc tcttaaaatc
   143641 tctctttgat tatggatagg atcataatca ccaaaatttt gccataatcc gagagataaa
   143701 atcggtaatt tcaaacctga atgaccggat ttacgatatt ccattttttc ataacgtgca
   143761 ctatctgcaa tatacaattt ttctcctcct catcaaatct ttttaaaacg ctttcttttt
   143821 tctattttta gtttactaca ttttaaagaa aaggagttaa tcaaaatgat tttttcgtat
   143881 atttttttgc catttgtttt ttactcgttg atagatcaga aattgattac gtgcttgctg
   143941 gattttttta taacgtttcg gttgtttttt gtaaaattgc tgatgatagt tttctgctgg
   144001 ccaaaacgta ccggctggtt cgatttttgt gacgattggt tggtggaaag ttcctgaact
   144061 gtctagtttc tgttttgatt gttccgctaa ttctttttgt cgttcgactt gataaaaaat
   144121 cactggacga tattgcttcc ctctatcttg aaattgtcca aatgtatctg tgggatcagt
   144181 aatttgccaa tagattgtca gcaactcgct ataacttatt tctcttgtgt cataaatgat
   144241 ctccacagct tccacatgac ctgtgtaacc accacttact tgatcataat ttggtttttc
   144301 aacatgtccg cccgtgtatc ctgacaaaac agataggatc ccttttttcg attcaaacgg
   144361 ctcgaccata caccagaaac atccccctgc aaatactgca cgttcttgat agtcagcatc
   144421 ttgcatggca tgcttggaaa agtcatattt ttgctcctta tcaaatcctt ccgtaatcaa
   144481 taaataaaag tccattacat caggcgtcag attcattcgg atagctaatg gacgtagtgt
   144541 tgcttctagt tttgataact gttctttaac ggatatattt tctcttatgt cattctttgt
   144601 ctgaatcaat acttttcgtt cccaatctcg tgtattcgga ttcaaaatca gattataaag
   144661 tgtttgaatg aatatttgtt cctcatccat agattctcct cctttccgaa gaagacaagc
   144721 tagtgacttt agctgtaatt tatcataaag tgaggaaata agcgattact tcatccccct
   144781 acagattttt cgtataaaaa agagagaatc cttatcgaca gattctcctt tttaatcaat
   144841 ttattttata aatgaaaagt taatttacct tttatggaaa actattttaa aaaataaaca
   144901 taaatatgca gttaatgtaa caagataaga aataatagtc gttactgtat aacttgtgct
   144961 tactttaata tcagcaactc ctttgactcc gcttatttgt accaatccat tagcattcac
   145021 ctgctccttt acaggttgat gatttatttc tacattcgtt cctaaataag catatacagg
   145081 aagtgtgatt acttggctct tctcattagt tactctatat atgatagaac taccagttgt
   145141 tttataagga aggtttaccc attgactgcc aatctttgct tcgtgtttta ttatatgctc
   145201 cttattctcc aatgaagcga taggagaata atcatataaa aaataatcca ttcctttagt
   145261 ggtatacgcc tctactgttt ctttgtttac aacaatatgt cccggctgag aaattatttt
   145321 ttgattgaaa ttttttttac tagaaacagt taaagtgatt aataacaaaa cagtcataaa
   145381 taaataaaat agttctcttg tcttattgta tcttctattt ttgaaaaata tcatcgttaa
   145441 tatcagagaa gagaatatta tttgcaagct taagatccgc caaggaaatt gtaacaattt
   145501 ggctggcgta ttttgcagaa gataccatgg caccaaacta gttgtagcta gccaagtcaa
   145561 acacgtacca taataaatct ttttttctat gtttgttaat tttttaaata aaatcagggg
   145621 aatacataaa gataacagac caactagtcc cattgagtaa gtagttaagt cgttgttcat
   145681 agcttcagtc agactatcga acacattaga tgcatgagca tataaatttg tcacgctagg
   145741 tttgttgatt ttttggtaga gcatttgttc aaacatcgga taccaaaaag cactcgtcag
   145801 aaccaatgtc attcctgccg cactaaataa ttttttaaat atagcaataa aatctgcatt
   145861 cttgaaacct ttagaaacca aacgaataaa aataaaaatg ataattaatg ctgtactcat
   145921 taatgttgat agtacatggc tgtacactag caaactcata ctcaatgcta gctttacaga
   145981 cggatacttt tcttcttttc cagagatgat ttgataagca taaaataata cagggataac
   146041 aagtgttatt gcgatagctt cggcaattgc ggaacgatag tagatgtcta ctgtccgata
   146101 agcagagaaa atatagagac aactaaataa aaatgccgct tttttttgtt ttaaaaaccg
   146161 ctcgccataa tgataagcta cgagtgttgt acacaagttc aataaccaaa cgtaaataat
   146221 atagcttaca atcaaatttt ttgtcatcca atataacatt acagctggaa aaaaagtcag
   146281 aaagggataa aaataattta caccatatcc agtataattg aatgtctcat aattaatcgg
   146341 actggtaaaa atagtcgata accccttgat tcgagataag tgaaaagaaa gatctccagt
   146401 ggtataaata ctctgtgtat ttttgaaaaa gatgaaagaa tatatactac taactataat
   146461 taatccactc aatattacta cttttttatt tattttcatt cttcttgttt atctcctttt
   146521 tagtcacata tcgagaatct ttcaacttat taggtgcaaa tcgtttaaaa cttgaataca
   146581 ccactaaaca ataagtcaga atactgatcc ataaagccat gagagcagtt ggagtatatt
   146641 ttgttgtaat ttctatctta ttttctccag catttacatc cacattcact aagccatgtt
   146701 ttttataaga gtcatttacg actttaccgt ttacttttac ctgtgtacct aaataagcat
   146761 atactggtac tgtcactttt tgctttttgt tgcttgccac tgtgtaagta atgttattat
   146821 ctgcagcttt atacttccct ttttcccacg attctcctac ttttacttca tgtttttcta
   146881 agtgaggcct ttcttttatc gcttctgttg gggcgtaatc gtataatcct cccatatctg
   146941 atgttgtata acgtgggagt gtttcttcat tgataacaat atgaccgtgt tttgattgga
   147001 taacttttgc gtaattttct tttgctgcta tagacgtgac gagtagaaac accacagtag
   147061 cgccaatgat taaccatgaa tattttttat tcatagtctt tttcataaaa acaagcgtta
   147121 tgatcattga agaaaaaaag atttggatac ctaagatacg ccaaggaaac tggagtaagc
   147181 ttatcggtgt tttttgaaac aaaaaccatg gaaataaatt agttacgcat acccatgtag
   147241 aaactacgcc aacatagatg attttttctt ttttctccat ttctttcagt atgaataaag
   147301 gaaaaatcaa cgcaaccaat cctaataacc ccatagtgta tgttgtaata tcgttattta
   147361 acgcttgaat gatactatct gaaatagaaa gtgcttctag ctgcaaatct gttttgaagg
   147421 gactgtgtat ttcttgataa ttcatctgct gcaacatagg ataccaaaaa tagctagtta
   147481 atgcacaagt caaaaaaaca gcctttacta aattcacgac cacaaatttt atatcgtttt
   147541 ttatttgctt gcttaaaaac aaacgaacta taatcataca agcaatcaat gcactacaca
   147601 atagtgtagt taagacatga gtatacacaa gtagacttat actgacagat aaagcgatga
   147661 cttttttttc tctattgaat atcaatccat aagcataata taaaacagct gggataatcg
   147721 taagagcgat tgcttctgca attgcggatc gataatagat atcaactgtt cgataagttg
   147781 caaaagtata taaacaacta aataaaaaag cagcatctat ctttttaaaa aacttttctc
   147841 cataataaaa actaatcaga atcgtacaaa tatttaataa ccaaatatat agaacataac
   147901 ttaatattaa gttatttgaa attccataaa aaacgactgc aggaattatt gtaagaaaag
   147961 gataaaaata gttcagtcca tctccataat tgttaaatgt tgtatagttg attggccctt
   148021 caaaaataga agataaccct ttgattcgtg aaagatgaaa agataaatca cctgttgtat
   148081 aaatttcttg agcatttctc ataaaattta ctgaatataa agtactaagt aagataagaa
   148141 ctactaaaag aaatgctttt ttacttattt tcatagattt gttgttctcc ttttttaatt
   148201 ctattttcag tatactagaa ttgtcttttt ttaataagtt ttgcgtta
//
LOCUS       DAJSBN010000002        87928 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJSBN010000002 DAJSBN010000000
VERSION     DAJSBN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.002"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(553..2166)
                     /locus_tag="ONT86_000131"
     CDS             complement(553..2166)
                     /locus_tag="ONT86_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2350959.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..3215)
                     /locus_tag="ONT86_000132"
     CDS             complement(2307..3215)
                     /locus_tag="ONT86_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2350960.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3398..4522
                     /locus_tag="ONT86_000133"
     CDS             3398..4522
                     /locus_tag="ONT86_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2350961.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4749..5666
                     /locus_tag="ONT86_000134"
     CDS             4749..5666
                     /locus_tag="ONT86_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2350962.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5659..6492
                     /locus_tag="ONT86_000135"
     CDS             5659..6492
                     /locus_tag="ONT86_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2350963.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6575..7693
                     /locus_tag="ONT86_000136"
     CDS             6575..7693
                     /locus_tag="ONT86_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR2350964.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7687..9585
                     /locus_tag="ONT86_000137"
     CDS             7687..9585
                     /locus_tag="ONT86_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCR2350965.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQISEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9579..10802
                     /locus_tag="ONT86_000138"
     CDS             9579..10802
                     /locus_tag="ONT86_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR2350966.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10888..12822)
                     /locus_tag="ONT86_000139"
     CDS             complement(10888..12822)
                     /locus_tag="ONT86_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2350967.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13048..13704
                     /locus_tag="ONT86_000140"
     CDS             13048..13704
                     /locus_tag="ONT86_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR2350968.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13778..14131)
                     /locus_tag="ONT86_000141"
     CDS             complement(13778..14131)
                     /locus_tag="ONT86_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2350969.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14323..15252
                     /locus_tag="ONT86_000142"
     CDS             14323..15252
                     /locus_tag="ONT86_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR2350970.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15263..16099
                     /locus_tag="ONT86_000143"
     CDS             15263..16099
                     /locus_tag="ONT86_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCR2350971.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16347..17123
                     /locus_tag="ONT86_000144"
     CDS             16347..17123
                     /locus_tag="ONT86_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2350972.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17299..18042
                     /locus_tag="ONT86_000145"
     CDS             17299..18042
                     /locus_tag="ONT86_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2350973.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18035..19615
                     /locus_tag="ONT86_000146"
     CDS             18035..19615
                     /locus_tag="ONT86_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCR2350974.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19866..20345)
                     /locus_tag="ONT86_000147"
     CDS             complement(19866..20345)
                     /locus_tag="ONT86_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR2350975.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20361..21113)
                     /locus_tag="ONT86_000148"
     CDS             complement(20361..21113)
                     /locus_tag="ONT86_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR2350976.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21230..21300
                     /locus_tag="ONT86_000149"
     tRNA            21230..21300
                     /locus_tag="ONT86_000149"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21262..21264,aa:Gly,seq:ccc)
     gene            21661..22386
                     /locus_tag="ONT86_000150"
     CDS             21661..22386
                     /locus_tag="ONT86_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR2350977.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22641..22907
                     /locus_tag="ONT86_000151"
     CDS             22641..22907
                     /locus_tag="ONT86_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCR2350978.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22904..23335
                     /gene="ruvX"
                     /locus_tag="ONT86_000152"
     CDS             22904..23335
                     /gene="ruvX"
                     /locus_tag="ONT86_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR2350979.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23360..23665
                     /locus_tag="ONT86_000153"
     CDS             23360..23665
                     /locus_tag="ONT86_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCR2350980.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            24234..25071
                     /locus_tag="ONT86_000154"
                     /pseudo
     CDS             24234..25071
                     /locus_tag="ONT86_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            25136..25570
                     /locus_tag="ONT86_000155"
     CDS             25136..25570
                     /locus_tag="ONT86_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2350981.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(25638..26960)
                     /locus_tag="ONT86_000156"
     CDS             complement(25638..26960)
                     /locus_tag="ONT86_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCR2350982.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(27114..28754)
                     /locus_tag="ONT86_000157"
     CDS             complement(27114..28754)
                     /locus_tag="ONT86_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCR2350983.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            29084..29554
                     /locus_tag="ONT86_000158"
     CDS             29084..29554
                     /locus_tag="ONT86_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCR2350984.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            30183..31286
                     /locus_tag="ONT86_000159"
     CDS             30183..31286
                     /locus_tag="ONT86_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR2350985.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            31570..32250
                     /locus_tag="ONT86_000160"
     CDS             31570..32250
                     /locus_tag="ONT86_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR2350986.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            32340..33374
                     /locus_tag="ONT86_000161"
     CDS             32340..33374
                     /locus_tag="ONT86_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR2350987.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            33388..33723
                     /locus_tag="ONT86_000162"
     CDS             33388..33723
                     /locus_tag="ONT86_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2350988.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            33707..36187
                     /locus_tag="ONT86_000163"
     CDS             33707..36187
                     /locus_tag="ONT86_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2350989.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            36243..37802
                     /locus_tag="ONT86_000164"
     CDS             36243..37802
                     /locus_tag="ONT86_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2350990.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDCPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            37799..39163
                     /locus_tag="ONT86_000165"
     CDS             37799..39163
                     /locus_tag="ONT86_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2350991.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            39334..40404
                     /locus_tag="ONT86_000166"
     CDS             39334..40404
                     /locus_tag="ONT86_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2350992.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            40419..41087
                     /locus_tag="ONT86_000167"
     CDS             40419..41087
                     /locus_tag="ONT86_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2350993.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            41107..41685
                     /locus_tag="ONT86_000168"
     CDS             41107..41685
                     /locus_tag="ONT86_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2350994.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            42088..42999
                     /locus_tag="ONT86_000169"
     CDS             42088..42999
                     /locus_tag="ONT86_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR2350995.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(43255..44172)
                     /locus_tag="ONT86_000170"
     CDS             complement(43255..44172)
                     /locus_tag="ONT86_000170"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCR2350996.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            44374..44811
                     /gene="zapA"
                     /locus_tag="ONT86_000171"
     CDS             44374..44811
                     /gene="zapA"
                     /locus_tag="ONT86_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR2350997.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            44869..45399
                     /locus_tag="ONT86_000172"
     CDS             44869..45399
                     /locus_tag="ONT86_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016630349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCR2350998.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            45531..47891
                     /locus_tag="ONT86_000173"
     CDS             45531..47891
                     /locus_tag="ONT86_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR2350999.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            47978..48292
                     /gene="trxA"
                     /locus_tag="ONT86_000174"
     CDS             47978..48292
                     /gene="trxA"
                     /locus_tag="ONT86_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCR2351000.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            48474..50252
                     /gene="uvrC"
                     /locus_tag="ONT86_000175"
     CDS             48474..50252
                     /gene="uvrC"
                     /locus_tag="ONT86_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR2351001.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            50397..51296
                     /locus_tag="ONT86_000176"
     CDS             50397..51296
                     /locus_tag="ONT86_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2351002.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            51289..52551
                     /locus_tag="ONT86_000177"
     CDS             51289..52551
                     /locus_tag="ONT86_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2351003.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(52645..53937)
                     /locus_tag="ONT86_000178"
     CDS             complement(52645..53937)
                     /locus_tag="ONT86_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2351004.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            54221..56119
                     /locus_tag="ONT86_000179"
     CDS             54221..56119
                     /locus_tag="ONT86_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCR2351005.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(56418..57137)
                     /locus_tag="ONT86_000180"
     CDS             complement(56418..57137)
                     /locus_tag="ONT86_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCR2351006.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            57474..57806
                     /locus_tag="ONT86_000181"
     CDS             57474..57806
                     /locus_tag="ONT86_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351007.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            57793..59796
                     /locus_tag="ONT86_000182"
     CDS             57793..59796
                     /locus_tag="ONT86_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCR2351008.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            59815..60285
                     /locus_tag="ONT86_000183"
     CDS             59815..60285
                     /locus_tag="ONT86_000183"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCR2351009.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            60306..60890
                     /locus_tag="ONT86_000184"
     CDS             60306..60890
                     /locus_tag="ONT86_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCR2351010.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            60905..61891
                     /locus_tag="ONT86_000185"
     CDS             60905..61891
                     /locus_tag="ONT86_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCR2351011.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            61881..62192
                     /locus_tag="ONT86_000186"
     CDS             61881..62192
                     /locus_tag="ONT86_000186"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCR2351012.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            62214..63995
                     /locus_tag="ONT86_000187"
     CDS             62214..63995
                     /locus_tag="ONT86_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCR2351013.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            63988..65364
                     /locus_tag="ONT86_000188"
     CDS             63988..65364
                     /locus_tag="ONT86_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCR2351014.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            65371..66006
                     /locus_tag="ONT86_000189"
     CDS             65371..66006
                     /locus_tag="ONT86_000189"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCR2351015.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            66036..67391
                     /locus_tag="ONT86_000190"
     CDS             66036..67391
                     /locus_tag="ONT86_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCR2351016.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(67583..68977)
                     /locus_tag="ONT86_000191"
     CDS             complement(67583..68977)
                     /locus_tag="ONT86_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR2351017.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            69069..70040
                     /locus_tag="ONT86_000192"
     CDS             69069..70040
                     /locus_tag="ONT86_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCR2351018.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            70037..70819
                     /locus_tag="ONT86_000193"
     CDS             70037..70819
                     /locus_tag="ONT86_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCR2351019.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(70988..71359)
                     /locus_tag="ONT86_000194"
     CDS             complement(70988..71359)
                     /locus_tag="ONT86_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2351020.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            71493..71936
                     /locus_tag="ONT86_000195"
     CDS             71493..71936
                     /locus_tag="ONT86_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCR2351021.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(71979..72788)
                     /gene="yidA"
                     /locus_tag="ONT86_000196"
     CDS             complement(71979..72788)
                     /gene="yidA"
                     /locus_tag="ONT86_000196"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR2351022.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(72807..74174)
                     /locus_tag="ONT86_000197"
     CDS             complement(72807..74174)
                     /locus_tag="ONT86_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2351023.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(74158..74988)
                     /locus_tag="ONT86_000198"
     CDS             complement(74158..74988)
                     /locus_tag="ONT86_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCR2351024.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            75151..75588
                     /locus_tag="ONT86_000199"
     CDS             75151..75588
                     /locus_tag="ONT86_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCR2351025.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            75664..76284
                     /locus_tag="ONT86_000200"
     CDS             75664..76284
                     /locus_tag="ONT86_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCR2351026.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            76473..76979
                     /locus_tag="ONT86_000201"
     CDS             76473..76979
                     /locus_tag="ONT86_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCR2351027.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            76996..77895
                     /locus_tag="ONT86_000202"
     CDS             76996..77895
                     /locus_tag="ONT86_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCR2351028.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            77982..78866
                     /locus_tag="ONT86_000203"
     CDS             77982..78866
                     /locus_tag="ONT86_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR2351029.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(79058..79129)
                     /locus_tag="ONT86_000204"
     tRNA            complement(79058..79129)
                     /locus_tag="ONT86_000204"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(79094..79096),aa:Arg,seq:ccg)
     gene            79189..80523
                     /gene="rpoN"
                     /locus_tag="ONT86_000205"
     CDS             79189..80523
                     /gene="rpoN"
                     /locus_tag="ONT86_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR2351030.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            80672..81763
                     /locus_tag="ONT86_000206"
     CDS             80672..81763
                     /locus_tag="ONT86_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCR2351031.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            81804..82805
                     /gene="gap"
                     /locus_tag="ONT86_000207"
     CDS             81804..82805
                     /gene="gap"
                     /locus_tag="ONT86_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR2351032.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            82915..84105
                     /locus_tag="ONT86_000208"
     CDS             82915..84105
                     /locus_tag="ONT86_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR2351033.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            84209..84964
                     /locus_tag="ONT86_000209"
     CDS             84209..84964
                     /locus_tag="ONT86_000209"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR2351034.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            85080..86378
                     /gene="eno"
                     /locus_tag="ONT86_000210"
     CDS             85080..86378
                     /gene="eno"
                     /locus_tag="ONT86_000210"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR2351035.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            86777..87916
                     /locus_tag="ONT86_000211"
     CDS             86777..87916
                     /locus_tag="ONT86_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCR2351036.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        28900 a        15226 c        18142 g        25660 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac tcctcaagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
     2701 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
     2761 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
     2821 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
     2881 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
     2941 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
     3001 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
     3061 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
     3121 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
     3181 ttggaggatt ggttgtttgt taacaaaaga accactttcc tttctctttt tatataaaaa
     3241 gctttctatt tataattaat gactattata acaaaaatga aaagccaaaa cgacaagtaa
     3301 aatatttttt tatataaata tttgtgttac taattccgtt aatatacata agtgaaagcg
     3361 ttttatataa gaacttgaaa agaaagaagg gatagacatg aaaaaaagat acaaattggt
     3421 gatagctttg ctaaacatta ccctattatt cagcttaagt gcatgtggaa aaactaacga
     3481 agaagtaaca gcaaacatcg cagatccaat gataaaaaaa gccactacat tagaagtata
     3541 tgtcgcaaac ggcaaactga caaactatgt tgaaaatgct gcaaaaatct ataaccgcaa
     3601 aactggagca aatattgact taaaaattgt gaatgtagct tcgggaacag caacagtaca
     3661 aatgatcacc ccaaaattgg taacacacga agatatgccg gatattatct ccatggcaga
     3721 cacttcagca gcaggtgtgc tggaaaaatt tcaagatgct ttttatagtg ctgatgaata
     3781 tggattttat gacaaatatg gcgatcagtt ttatgatcaa aaattgaata ttttgcgtca
     3841 acaaacgagt aatgaaacga ttattccttg ggctagtgat tttaccccag cactttgtta
     3901 ttatcaacca aaaaaatttg ctgaaatagg tgtagatttt gaagaaatca gttcttggga
     3961 agaatatatc caggtggcaa aagcattaaa agaaaaaact ggtagttttg gaattgcctt
     4021 gccagaatct ggggaccaag aattgtttat caacatgcta gcccagcaga atcaatcttt
     4081 gcttgatgaa gaaggaaata tccgtctgaa tactaaagaa gcgaaacatg ctgcaaagtt
     4141 aatcaaagaa atgatcgact cagatattgt acatttctac ggtgcacaag atgctgagaa
     4201 agcatttcaa gaatctgcaa tgtttgttgc tggcggctgg tatgctacga atatgtcttt
     4261 gaattttcct gatgcatctg acaaatggcg catggctcca ctgattcctt tttcaaaaga
     4321 aaatccagga aaaggtgcag tatcaggagg atcaagtttt tatgtcccta aagatgccaa
     4381 aaatccacaa gtggcacaac aatttcttac atttatgctg acagatgaag aatgcttagc
     4441 caacgcactg gagctgggcg tggctacatc taactcattg gcctatcaaa cagaagcagc
     4501 tgagaaaaag tttgactact aaggaggaca aaaatactat gaactattga ataaaatcaa
     4561 tgaaaataca tcaaatactg ttttaccagc cacttattca gatgctacgt cttatttgac
     4621 taccgctgca tataactatt ggcaaacagg tgattttgaa aattcatatc taaaagaagc
     4681 agaaaatttt gctcataagt acggcgtaaa attaactggt gattaaaaaa tattaggagg
     4741 acttaacgat gatgcaatct tcgttaccaa gcaagacaat aaagaacagc aagaaaaaag
     4801 caacagatac gtctcataaa aaaagtgctt atggattggt cacacctttt gttattttct
     4861 ttattgtgac gattttgatt cccatcatta tctcgtttgc ttttagtttt atgaaaattg
     4921 gctactcatt tgaatttgtt ggatttgaca attatctagc tatcttccga gatccattgt
     4981 ttattaaatc ttacacgaat gttttgatta tcatggcagg atcaattcca ataacgatgc
     5041 tattagctat ttactttgct gttttattga attcagcaca gttaaaagga aaaggatttt
     5101 ttagaactgt ctattatatt ccaaccatta catcaacggt ggctgtagct tccgtattca
     5161 tgactttttt caatccgact gggttattca atggattttt gaatgcgcta ggaattaacg
     5221 gtgtacagtg gttaacaaat cctttttgga tacgaatatc aatgatcatc acgatgattt
     5281 ggcttagtgt aggatataac actattttat ttttagctgg tcttcaaggg atttctaaag
     5341 agatatacga aagtgctgaa atagatggag catcgaaatt tcggcaattc tttttattaa
     5401 cgatccccat gctaaagcca atcattttaa tggccacagt tttagccact atcaatggct
     5461 tgggtaattt caatatccca aatatattct ttgggacttc caatggacca gaaaattcag
     5521 ctttggtcgt aggagtcaat ttatataaaa caagttttga aatggttgat tttggaaaag
     5581 cctctgctat tgcctggacg atggtgtttg tatctgttat attatcggct attcaattta
     5641 aattcggagg tgataaaaat gactaaacaa aagatacaaa gcagaatttt tacgtacgtc
     5701 attattgtgt ttggtgcctt aatatttttg atgccatatt tatatatgat tgtagcttca
     5761 acgcaaagta atcaagaaat tttaagtggc ggaatcaatt tcaagtttgg ggatatgcta
     5821 ttaaaaaact ggcaagccat catggcgaga tataattatc ccttgattat ttggaattcg
     5881 attgttattg ctgtaatcag tacagtttta gctacagcca gttctacaat ggcgggttat
     5941 gcattagcaa agtatcgttt tatggggaat aaactgttgt tcaatacagt tatgctatct
     6001 aggatggtgc ctttttttac cacattgatt cctttatttt acattatgag tcgtttagga
     6061 ttatcgaata cttatgcagg tgtgatcatc ccttcgattg ccagtacaac ttcagtgttt
     6121 atgatgcgac aatatagcag tcaatttcct acagcactga tggaggctgc cagaatcgat
     6181 ggtgccaatg agtgggtgat ttttacgaaa atcgctgtac cgattttatc acctacgatt
     6241 attactagtg gtttacttat ctttatggga agttggaatc aatatctatt tccattagtc
     6301 atgctttcag ataaagataa atatacggtt cccttaatta tcaaaaatat gacggtttct
     6361 agtacaggtg agccaattaa ttatggcgca attatgctaa ttttagcaac ttcagtgatc
     6421 ccaatggtct tgatttatgt gtgggcgcag tctaaattta aggagaatga cattggagct
     6481 gcaaataagt aaaaaaaata acgaatgtta accatctagt tcgttctagg agcaactcca
     6541 cattcataaa aattaaaaga atggcgggat atccatgaat gaacagacga aggaatggtc
     6601 aagaaaaatt ttgaatgtag cttctgaaaa gatagatgcg gaattagaca gaatggggaa
     6661 taaaattcca tatatttctt acaacggaaa atatacagac gtaataaagg acagaggaat
     6721 cgactggtgg accaatggtt tttggagtgg tattttatgg cagctctttc acaagacaaa
     6781 acgagaaaag ttcaaagagg cagcaataca acaagaacaa ttattagaat ctgccctgta
     6841 ccatttttca gacgttcatc atgatgtagg ttttttattc ttaccatcgg ctattgcaca
     6901 ttatcaacag tctcaaaaca aacatagtcg acaactaggg ttacatgcag ctacggtact
     6961 tgctggacgc tataatattt cagggaacta tttagtagca tggaatgacc ataaacctgg
     7021 ttggatgatt attgatagta tgatgaattt accattactg tattgggctt cgagggaaac
     7081 tggtgatccg aggttctcta aaattgctcg gcagcatgca gatacaacag ctaagtatct
     7141 agtgaggaaa gatggatcaa ccgggcatat cgcagaattt gatgcagaat cagggaaata
     7201 tattcggtta cttggcgggc aaggatacaa tgagaattct tgttggagca gaggaaatgc
     7261 atgggcttta tatggattcg cgttaagtta tcgttacaca aaaaatccgt actatctagc
     7321 tatagcagaa aagatagcag taaattttat tcagaaaatc gaaaaaactg gttatattcc
     7381 tctcattgat tttgctgtaa aagattcgaa tattgatacc agtgctggag tatgcgctgc
     7441 atgcggtctt ctggaattag caggatatgt ttcctctgta cgatcaaaaa tgtatcatga
     7501 agctgctatc aatatactcc aaaagacaga aaagcactat gcaaattgga atatggatga
     7561 agatgggatc attggaggcg gagcagaagc ataccataga gaactgactt ataatgtgcc
     7621 actgatctat tctgattatt tttttattga aggcttactt cgattagaac agtcggaatt
     7681 agctatttgg tagtaaaaag aggagaaaat acaatgaaac atccagtacc actattaagg
     7741 accagacagg attactttga cttatttaaa aagataaatc aaccactcca gccgttttat
     7801 cgagcaagtg gcgcacaaat tgattatata catcaagggg taggatatgg aaataaaatt
     7861 gcaggaatgg aaggattctc tcgggtttta tggggagcgg ggcccgcaat tgaccaatta
     7921 gatgaacgat ggttgaaatt aattctatca ggaataaaag ctggaacaga tccttcccat
     7981 cccgattatt ggggagaaat acatgaaaga gaccagcgta tggtagaaat gcccgcaatg
     8041 ttattagcgt tatatcatca tgaacaatta ttatggaaaa aattagatac aggagaaaaa
     8101 agccagatta ttcattggtt ctcgcaaatt tctgaatatg aatgtgcaga tggtaattgg
     8161 caatttttta agataattgt cggaacaatt atccaaaaat ttggttatcc agttaaagaa
     8221 tcagcactga aagaagcatt tgataatata gaaaattgtt atcttcaaaa tggctggtat
     8281 cgagacagtt caagaggtcg ccaagattat tataatcctt ttgcatttca ttattatggt
     8341 ttgatttata gcgtactaga accagaaaag cccattagcc agatatataa gaaccgagct
     8401 attgattttt ctagagacta tattcatttt tttgcggaag atggtgcaaa tgtgcctttt
     8461 ggtcgaagca tgatttatcg ttttgcagtt tcaagctttt ggtcagctat gttgtgggcc
     8521 gatcttttgc cttttaaatt aggtgaaatc aaggggttag tcaatcggaa tataagatgg
     8581 tggatgcaaa aagaaatatt tgatgaaaaa ggtatcctga atattggtta tacctaccct
     8641 caattgacat tgactgaacc ctataatagt acgttatctc cattatggct gaataagatt
     8701 tttttattgt tggcgctacc tgaagaccat gagtattgga cggctaaaga aatgcctatg
     8761 cctattcgtg aaggaactaa acttctttca gaagccaatt tgctcataca acatgataat
     8821 ggacatacat tttttttgaa tgcgggacaa ttagggccaa atttccatac attgaccaat
     8881 gaaaaatatt taaaatttgc ttattctagt caatttggtt tttcgatacc gcgagcgaat
     8941 caattgaaag cagaaatggc aatggattca atgctaggga ttcagcgagc ggacacatat
     9001 atcacgacca gttttaagaa tgagagcatc aaaacctatg gacagttcat cgttcgaaat
     9061 aatgtacgag acattgtatg taagaaagag gtacttgcct ctacatggga acctacttta
     9121 caggcaaaaa tcagaacttg gcttattcct tttgaaggct ggcagattcg cgtacacaaa
     9181 gtagaattag atacagaata tgttctatat gagacagggt ttgctattga atgttctcct
     9241 gagaaagaag gaataatcat agaagaagac aaggaaaact atagggtgag tgaagctggc
     9301 ttttcaggta ttatctgttt aagtgacgat actattaaac gaaaatccac agctattatg
     9361 ggtttgccca atacaaactt gatgacatgg gaaaacactt ttattcctgg attagagggt
     9421 acatttggaa aaggaaccca ttggctcgga acaggagtgg ttgctcatca agatcgggat
     9481 tatagtttag aagtatggaa caatcgtccc aaaattgatt ttgatgggac aaccgggcta
     9541 actatccaaa ataaaaacaa taaaaaaaga ctaaaattat gctaaaggct ggtggggaaa
     9601 tgcttgataa agtgatgttt gaagagataa tacatccaga aaaatggata aatgaatgtt
     9661 ataaagaatt aatggaaaaa gaatggaata atgagatcga gcgttgtcgc agacacacgg
     9721 agaggttaat caaacgtatc agtaacagtc agtatcaatt cccaagcgag tgtcctataa
     9781 aaggacgtta tccattaaga gacaatattg gttggacaga aggattttgg acaggaatta
     9841 attggttgtt atttgagaag acgggtgatc ctttatttca acaaacagct gaaaaagcag
     9901 atatagattt tatccacagg attgactgtc aaattatcac taatcatcac gatcttggtt
     9961 ttttgtattc tttatcaacc gtagcagatt ataaaataac gggaaatcga aaaatgaagg
    10021 aagcttcaat tgatgctgca gaaacattac ttcagcgttt ttgggagaag gcaggagtta
    10081 tacaagcttg gggagatcct agtgatcctt cacaacaagg tagaatgatc atggattgtt
    10141 gcatgaatct tccattattg tattgggcga gtgaagaaac tggaaatcaa aaatataaaa
    10201 aagctgctca ggctcatatt caaaatgcag cagattattt agtaagagaa gatggttcaa
    10261 cttatcatac attttatatg gatgtcaaag atggaaaacc aagatatgga agtacgcatc
    10321 aaggttatag tgataattcc tgttgggcta gaggccaagc atgggggatt tacggttttg
    10381 cattaagtta tcgttatttg aaagaacaaa aatattttga tttaagcaaa atattagcca
    10441 attattttat gaatcggttg ccagaagata ccatttgtta ttgggatttg atctttgatg
    10501 attctagcag agaagaaaaa gatacatcta gtatggtgat catggtttgt ggattattag
    10561 aattaataga atcaggtcta ttagacaaaa cagaaaagaa cttataccag tctgctgttt
    10621 ggaaaattat gcgacaattg aaagaaagat atgctacaac ggaagaagaa gatggctttt
    10681 taacacagtc tgtttataat aagcccaaaa ataatggggt aaatacacct tgtatttggg
    10741 gtgattacta ttatttagaa gctttagtga gaatgactag taattggtct tcttattggt
    10801 gaaaaaggta aatatggaat ttttgtttga taaaataaaa aaagaaaaac caaaacagca
    10861 tgaatggttt ctgttttggt tttttcgtta attttcatat tcttctaatt ccaagcttag
    10921 ttcttcccat tccgtcaatt ttgtttcttg atcttgtcta gcagcttcta aatcttggtt
    10981 cagctcattg agttttacgt gatcttccag gttttctggc gcgaccattt cttgttccaa
    11041 ctgatcgatc cattcatcga tccgcgccat ttcttcttca acctgagtaa tttttcgttg
    11101 aaggctacgg ataagctttt gctgttcttt gctttggtaa taatcagatt tgttcgtggg
    11161 agcaggagca gattctggct catcttgtgc caacaattcg gcgatttctt cttcttcctg
    11221 ctttttctct aaataataat catagtctcc tagatagact ttgctgccat tttcagagag
    11281 ttcgacgact tttgtcgcga tccgattgat gaaatatcgg tcatgggaaa caaaaagcag
    11341 tgtgccgtca taatcgatca atgcattttc taatacttct ttgttgtcaa tatctaaatg
    11401 gtttgttggt tcatccaaaa tcaaaaaatt ttctttgttc atcgctaact tggctaaagc
    11461 aacccgtgct ttttcaccgc cacttagcag agggattggt ttttctacat cttcgccaga
    11521 aaataagaaa gcacctaaag cacttcggat ctctttttct ggagtggtag gatgctcatc
    11581 ccacaattct gctaggatcg tcttgtttgt atgcagatca gcttgacctt gatcgtaata
    11641 gccgatggtg acatttgtcc ctagcgtttc ttttccacgg ataaaaggga tctggtgaat
    11701 gattgatttc agcaacgtcg atttcccgat accattcgga cctactagag caatcgcttc
    11761 ttgacgacgg atatctagat tgattggttc agataaggtg acattatcat atccgatagc
    11821 ggcatcttcg atttgcaaga ctacatttcc agaaggcttt tctatactga atagaaagtt
    11881 tgcttgtttt tcatcccctt gaggacgatc cagccggtcc attttttcta atgtctttct
    11941 gcggctttgc gctcgttttg tggtagaggc gcgaacaagg ttccgagcaa cgaagtcttc
    12001 taatttatgg atttctgttt gctgcttttc ataagccttc cattcacttg ctaactgttc
    12061 tgctttcaaa tctagatatt tcgaatagtt acctttgtaa tggcgcattt ttcttctgct
    12121 taattcatag acttcattga ctactttatc taaaaaataa cggtcatggg aaacaatcaa
    12181 taatgcacca gtatagcttt gcagataatt ctcaagccag gaaagtgtct cgatatctaa
    12241 atggttcgtt ggttcatcta gaatcaaaat gtcagggtgt tgcaaaagca tgcgagctaa
    12301 agctaaacgt gtgcgctgtc cgcctgataa agtatcgatt ttctgatcat aaaatgaaga
    12361 atcgaattta aacccatgca atacggaacg aatttcattt tcataaccat agccgtttcg
    12421 atctgaaaaa tcgtgttgca gccggtcgta ttcttttaaa taagaactat aagcaggtgt
    12481 gtctggcgta ccttcgctaa tgaaaatctc tagctcgcgc atccgctctt ccatttgtct
    12541 aacttgttca aaagctttca gcatctcgtt ccaaactgtt tcattggaat caagtcctgt
    12601 gtcctgagcg aggtatccga gagtcgctgt tttattttta gcgatctttc cttcatcagg
    12661 tgcttcaatt ccagcaatga tctttaataa agtcgatttt ccagcgccat ttctgccaac
    12721 taaagcgaca cgagcgcctg tactgatttc taaatgtatg ttttcaaata agacttctgc
    12781 accaaaatgg cgagcaatct gattggcttg taataatatc atggaatcct cttttctact
    12841 ttgtgaattc tcctcaaata aaaaactatt tttcaattag tagtctacca taaattccat
    12901 tatactgcga gatttgcaga aatgataaat aaatgaataa agaataaaat ttcacaatat
    12961 ctgagcaatg gaattgattt tttcctataa atactgctat gatggagaaa tcaaagatgt
    13021 aattagacaa tacgtggagg tcatttcgtg aaagatcata ttattccaaa ggcgacagct
    13081 aaaagactgc ctttgtatta tcgctattta aggattttaa ataatgctgg aaagaaaaaa
    13141 gtgtcttcaa ctgaattaag tgaagccgtt caagtagata gtgcaacgat ccgtcgagat
    13201 ttttcttatt ttggcgaatt aggaaaacgt gggtatggtt atgatgtaga agatcttatg
    13261 cacttttttg caaagacatt aaacgacgat caattgacaa atgttgcgct gatcggtgta
    13321 gggaacttgg gaagtgcttt actaaaatac aaattccatc aaagtaatag catacgtatc
    13381 agttgtgctt ttgatgtgaa tgaggaaatc gttgggcgca tcgtagacgg tatccccgtt
    13441 tatccaatgg aagatatgtc agaacaaatc aagatccaac aaatcgaagt agcaatttta
    13501 acgatacccg ctgaaagagc acaagaagta gtcaatcagt taacggaagc aggaatcaaa
    13561 ggaatcttga actttaccgc tgctcgattg aatgctccgt cagatgtatt gatccaaaat
    13621 gttgatttga cgaatgaatt gcaaaccttg atctatttct tgcattctga cgcaaatgcg
    13681 cattccgaaa atacagcaga ataaaaataa aaagcgattg aacaaatgaa ggcaaaactt
    13741 tcacttgttc aatcgctttt ttctgtttgg acaacgatta ttttgttcgg aagatttctt
    13801 cataagaaag ggaatcatct tctgctgtca gttcatctag tagataacgg atttttttaa
    13861 agtcgatgaa ccgtgcttgc cgaaagactt cttttccttg atgaaaaatc aggacaactg
    13921 gcgcagtcag cacttcaaat ttactagcta cttctggtgt ttcaaatgca tccaaatgaa
    13981 gtgccggaat atcataatgt gtgagaagtt cctctagtcg tggacgaaca gcgtgacaaa
    14041 cactgcaatt aggcatcgaa agataaatga cggcaacgga tgactgttgc agtatgtctt
    14101 gaacttcagt caagttagtg gtttgtttca tttttattct cccttttatg atagaagtga
    14161 tatgtatgag accttaattt aatgggaata acagaaaaag tcaattgaag tgctttggta
    14221 aaaaataaga gttgtgtaaa aatttacaga gcaacttgtt ttttttgaat aaaagcgtat
    14281 atttgcaaag cgaatgaact agaaataaaa aggaggaaac tcatgttttc ttttattcca
    14341 aattcggttt tgcagatgag tacgattttc ttatcgatcg ttattgaagc attacctttc
    14401 gtattattag gctgtcttat ctcaggcgcc ttacaagtat ttttgacccc agaacgtgtg
    14461 aagagatggc ttcctgaaaa tcgtatcttc tctattttta ctggttcgat cttaggattt
    14521 ttctttcctt catgtgaatg cggcatcgta ccgatcgttc atcaatttgt taaaaaaggc
    14581 gtaccagttc acacagcgtt tgcttttatg ctgactgctc cgatcatcaa tccaatcgtg
    14641 attttttcca cttttatcgc atttggcaat tcttggcaga tggcaagctg gcgggtgata
    14701 ggcagctttt ttgttgccct tgtgattggt gtctggcttg cctattttca aaaagacgct
    14761 gtattgaaag aaaaaatcac gtctaaagta gcagaaaatc accatcatca tgtccatgaa
    14821 gaagaacaaa gtgagaagaa tgaaccaaaa aatcagctga tccatcaaac tgggcatgtg
    14881 ttgacacatt caatcgacga attttttgat acaggtcgtt atttgatcat tggcggatta
    14941 atcgcagctg cgatgcagac ttatttacca actagaatta tgctaacgct cggctcaaca
    15001 aagattttag ccattatcat catgctgtta ttggccttta cgatgtctct ttgctcagaa
    15061 gcagatgcgt ttataggatc ttctttgtta agtctgttcg gcacagcacc tgtagctgcc
    15121 tttttggtct ttggcccaat ggtcgatatc aaaaatctac tgatgatgaa acgatatttt
    15181 acaggacgct ttattttatc gctgatcgga ctgattgccc ttagcgttat tggattcaca
    15241 ttatttattt aggagggaaa atatgctgcg gtttttgatt ttatctgggt atttcgaatt
    15301 gatgatgtac ttgcaagttt ctggcaaact cgatcaatat atcaatgtcc attatcgcta
    15361 cttagctatt ctttcaatga ttttatctgc tcttttggct ttagttcagc tgtatttgtg
    15421 ggtcaaaaac ggaaaagcag aagaaaaggc acacgagcac gatcatgacc atgggttgtc
    15481 aaaaccttat caacgtggaa tcgcttatgt actgctagct ttgccagtaa tcgtggggac
    15541 gttgtttcca acagttagtc tcgacactac gatcgtagaa gccaaaggat ttaatttccc
    15601 agtaagtaaa gaatcagtag gggatccgga gatgcagaca caatacttaa agccggatac
    15661 cagtatttat ttcaacaaat cagactactt agaccaaatg aatgagttga aagaaaagta
    15721 tggggataag caaactatca agatcacaga tgaaaactat ttagaagtta tggagctaat
    15781 ttataattac cctagcgaat tcattggcaa aaaaatcagc tatgaaggat ttgtttacca
    15841 aacaccgaac gatagcaata cagatatctt cttgttccga ttcggcatta tccattgtgt
    15901 ggcagattca ggagtctttg gtttgttgac gcatatgccg gaaggagtaa cggttaaaaa
    15961 tgatgaatgg tataaaatag aaggaaccat ccaatcagac tattatcaac cattcaagcg
    16021 tgaaatccca tctgtagttg ttacaaacgc tgagaaagtc tctgctccga aaaatcagta
    16081 tgtgtatcgt tctttttaaa agaacaaaat aaataaacat agctcagaca gctgacacgc
    16141 aaggacgtcg gttgtctggc tttttttgca tgatgtattc atttgtttga tatttatttt
    16201 agaagcagca tcctgaaagt atttgatatc ggtttcaaag gtgttggaaa agattgtgaa
    16261 gagaattaca atttattcct aacatgtatg ttgaaaagtt gtatgaaatg attttgatag
    16321 taaaatatcg agtggaggta ctttttatgg taacgcgtat atttattttg gatgatagtt
    16381 tggtttttga aaaaatgcta gtagaaattt tggaagaatc cagaatttta ttaagtcctt
    16441 ggaaatttga gatcgttcag ggattgaacg ttatacaatt tgttgaattc attaaaaata
    16501 atgaaatcta ttcctcagat atttttttcc tagacatcga tttgaacgcc tattatagcg
    16561 gtatcgaact aggcgaaatg cttagagaaa agaatgaaaa gtgtaaaatt gtttatctaa
    16621 cgtctcatcc gaataaagca ataactgtta ttaatcgaaa gattacgcct agtggttatt
    16681 tgataaaaga attcacttat gaaaaaaata aaaaaagcat ccaaagttac ttattgaaag
    16741 aaaaagaaaa ctatcctgtt caacatgcta cgaaaaaaag gtggacaact gtccgttatg
    16801 gtaatagcga tcactatatc cattacgatg aaatccttta tctaatgagc atgccaggtt
    16861 ataaaaataa aataggccta gtattgaaag atgaagagat tctcgtaaac ggcacaatta
    16921 aaaaatataa aaaatcactc gaatatgagt atttatgtaa agatttaaaa gcttatatca
    16981 ttaatactga aaacattaca tctattagca gagtaaacga aatagtaaca tttgcaaatg
    17041 gagaacaact agagatggga aagagaagta ttgataaact aaagaatttt atgcaagaaa
    17101 agatagaaaa tgaatcattt taaagaaaac aaagactcat atcttacctg ttagaagagt
    17161 gttttcctgt agaagagaac aacccaagta gcagcctagt cgtttctatc cttttttgtt
    17221 atgcactctt tttgacattt actccaccaa taacgaaggg tacaataaaa agtaataaca
    17281 aacaaaagga gtacagaaat gattgaattc atcaatgtaa gtaaaagtta cgggaaaaaa
    17341 caagcattaa aagacttgtc tctctcgata agacaagggg aaatttttgg atttttagga
    17401 cataatggtg ccggaaaatc aactacgatt aaaagtttag ttagtattat tgaaccatca
    17461 tcgggaacga ttttagtaga tggaatgaaa ttgacggaaa atcgattgag tatcaaacaa
    17521 aaaatcggtt atgtgcctga ttccccagat atttttttac aattgacggc tggagaatat
    17581 tgggacttga tttcagctgc ctatgaatta gacacacaaa aaaaagaaaa gcgtttagca
    17641 gaattgacag cgctttttga tatgtatagt catcaaaatg aaacaatcgc aagtttttca
    17701 catggaatgc gtcaaaaaac gattttgatc ggcacgttgc tgccagatcc agatatttgg
    17761 gtattggatg aacctttgca aggacttgat cctcaagcag catttgattt gaaagaaatg
    17821 atgaaggctc atgccgcaaa aggaaaaaca gtcatttttt ctacacatgt attggatacc
    17881 gcacaacagt tatgcgataa actggctatt ttgaagaaag gtgaattgat ttatcagggg
    17941 gcagttagtg aattattgaa tggctcacct gatgaaacgc tagaaaagat ttatttgaaa
    18001 atggctggaa ggcaagcgac aggagatgga ccgtatgtat aagcgacaat tattagaatt
    18061 aattcgagtt aatcttttat atgttaatcc acagctcacg aataaagcaa gagagaaagg
    18121 aaaaaaggga aagcgattaa ctatctatct tttgtctcaa aatctttttt cagggctgat
    18181 ttttttactg atttacggat ttgctatgtt tgctatgaac tttgttaaat tgccaggttt
    18241 ctttacttat tacgtggcac tttttggtat gttaggttta actcaaagta tatcagtcat
    18301 ttataatatc ttttttgaag gaaatgacct gcaagtattt ttgcctctgc cttttggaca
    18361 agaacaggtt ttttcagcaa agattttggt agttgctttg accatcattc cttttgtttt
    18421 tccgcttcta gtcatgttta ttttaaccgg atggcgagct gatctaggga ttttgcttac
    18481 tatcattatc tcaatccttt tgttttctct atttttgatt attctttttt gtctggctag
    18541 tttgattgtt tttggtttgg ctcgaacagc agtgtttaaa aaacatagaa aaacagtttc
    18601 tagtatcttg cttggcagct cgatgctgat cgcatttgct ggaatcatgt ggatgaatca
    18661 tcaaacgggg actattgaaa atgagttaac agatcggcat ccgatttcga tattattgcc
    18721 attctatgat gctgccagtc agccactttc tcaacaaggt ttattgggtt ggggaatact
    18781 agctatcacg gctctgggat ttttccttgc tgtaaaacag ctgatagtac caaagcttac
    18841 agagcaactg actgattcaa acagtgagtt caaaataaaa agaacccata aaaagaacca
    18901 aaatgtgcat cagcttttat ttagttacaa tgctcaatta gtaaaagaac ctaatttgat
    18961 tatgcaagtt ctttcttctt ccttgttaac tccaatcatt tttattcttg cttttgccct
    19021 aggaggcgaa ataaaattaa ctgatttaga taataagtta atcggtgttg ttttcctggt
    19081 agggatcgca ttggctttct taacagttaa tcagacttct tttatcagca atcttatttc
    19141 tttggatcaa gaaaattttc tgtttatccg ttctttgcca atctctatga accaatattt
    19201 aaaagaaaag ttcagattcg gatggatatt gcagagtttt ttaactggtg ggatcgcatt
    19261 attaagcgga ctgtcttttc agctgccttt gttttttatc cctagcttgc tatttggttc
    19321 tttattcggc tgctacttat tgtcactacg ttatttcgct agagattatc ggttgctgct
    19381 gctgaactgg accaatatta accaattatt taacagaggt tcaggaagtt tgggtttagt
    19441 cgcaactatg atgggttcat tgatcatcag tattattctt atcgtcttat acggaatgtt
    19501 ggctttgttt tatccatttt ggttagtgaa tattcctgtg atcggtttag ttctaatact
    19561 ttccgctcta tggatcgttt attatcaacg aacgttttgg aaaaaatttg aataaagaag
    19621 aggggccgtg acaaaaatca tgtcgcagcc ccttttttac agatagaggg tgttcaaaag
    19681 tcagtttctc agtattaagt caattttatg taaaaatgtg agaagctatt tttacaaaac
    19741 tttttttaat actcataatt acttcttatt gctcctgcgg ttacttgtcg caatgtttgt
    19801 gagatatttt gtcatcgctc aaggctgaac gtctctttca caaccttttc tttgttacac
    19861 ttcgtttatt taaaaagccg tttttttcgt ttcatcatga ttgcgtcacg ctttactttg
    19921 aaattacgga tgttatggtt aaagacttct gataatacaa gacctaaagc gattgctcca
    19981 gcaatcataa ttgcttggac cgcataatga gccgcttctc gataggattc agtaacgaga
    20041 tttcgaaccg cttgataagc taaaccacca gggactagag gaaccatccc gggaatattg
    20101 aaaatcgtta cgggcatttt caaacgttta gagaaaagat cgctgatgaa tgctactcct
    20161 aaagaaccaa gcaatgaacc aaaagcagca gatccgccta tttgaacaca gatccagtag
    20221 atcatccagc caaaagcacc agaaagacca caacaaatca atgaacgcct aggtacattt
    20281 gtaataacgg caaatgccgc agtcgctaaa aaactaaaag aaaactgaat aaatatcgtc
    20341 cccatacttg tgttcctttc ctaataaaaa agctggaaaa taaaagcaat cgcaaaaccg
    20401 atcatcgtag cagtcatcat cgcttctgtt ccgcgagaaa ctcctgagac ataatgtcca
    20461 gctagcaaat cacggatagc gttcgtaatt tgaactccag gaactaaagg catcacacat
    20521 ccgataatga ttagatcctg attgcttcct aagccaaaac gtacgctgta gatagcggct
    20581 atgccaatca ataaagaaga taaaaattcc gataaaaatt tgattctaaa aaatttcaaa
    20641 ctatatagat aagctgcgta tcccaatcct ccaatgacta aagtcacagg taaatcggtc
    20701 atctttccgc caaataacac cataattgtt ccactgatca ctgccgcgct aacgatttgc
    20761 aaccagacag gaaagaaaga acggtctcgg tcgattgctt gcagttcgca gtaaagttct
    20821 tctaatgtta gctctgaagc aacatattta cgtgaaagat catttacttt tactactttt
    20881 tctaaattaa tcgaacggtt ggtaatttgt tccattcgga tggtcgaggt acgatcaaga
    20941 ccaataaaga gaccggtttg agtgacatag ctgaccaaac ggaaatttcc agaagctaaa
    21001 gcaattcgac tcatcgtatc ttctacccga tacatctccg cattgctttc catcatgatt
    21061 ttcccagcga gtaaacaagt gtccagcaac agatctaatt cttttcccgt catagcatct
    21121 tctccaataa ttgattgatt agttttattt tactacaaaa cataccagaa gcgtaaagaa
    21181 taaaaaaact tgaggaagga ataaaagagg cgtatactgt ttttgtattg cgggtgtagt
    21241 ttagtggtaa aatcccagct tcccaagctg atgtcgcggg ttcgattccc gtcacccgct
    21301 taaaataggt tgtgaaaaag ctgctctgca acaagtaata agaacagcca agcaaaaaca
    21361 acttcttatg tttttaactt ggatggactt attaccgcag atgcaagctt ttgcgcaccg
    21421 tttaccaagc ggttgtgaaa tcagtgtatt gtcttgagta tgaagaaaat ttttgaataa
    21481 taataaatac cttacagata gagtggtgac tgattagaga cgaggaatcc gtctcttttt
    21541 tgtattttta agccatctta aaacaggttt tattttttgc tgtttctatt tctttctagt
    21601 atttatttat aggggaacat atgttaagat ttagggagct gttagtaaag gagtaaagaa
    21661 atgagcaaaa aaacaaccaa ggcgatcgtg gggatcgtcg ctgctgtgat tgcaggggca
    21721 ttcggtgttt ccaccacaca aaaagattca gatatctttc agcagattac aactattttt
    21781 gattccacac cacaagcttc tcaagtagcg gatgtgccag aatatgatgg aaaacatcaa
    21841 gaaatagaaa tcaatgggaa tcagccggaa tttacaaaag aggatcttag cctttccaaa
    21901 ggaacatggg aaagctattc aaatattgat aaattgaaca gagtaggaca agccaatgct
    21961 atgcttggaa aagatttgtt ccctactgaa aagagagagc ctttgtatat tgacccgaca
    22021 ggttggaaac aaaagaaatt aagtgacggg caatggttat ataatcgcag ccatttaatt
    22081 ggtttccagc tgactggtca aaataacaac attaaaaact tgatgacagg gacacgttcg
    22141 ttgaatgctc catacatgct tgctcacgaa aatgatattg cggcatatat caaagaaaca
    22201 aatcatcatg tacgctaccg tgtcactcct cattttgagg ggaatgaact ggtagccagg
    22261 ggagtgcaat tggaagcaga aagcattgaa gacaaaaaaa ttgaattcaa tgtctttatc
    22321 tacaatgttc aagatggcta cacgatcaat tatgagacag gacaagcaac aaaaacaaaa
    22381 aattagtagc agctattcga attttttatg aatattatga aaaaattgtg taaaaacata
    22441 cattcaaaga aatatagcgt atactttatg aaaagagtac ttttgataaa gctacagaaa
    22501 aaaggaaaaa aacatacaaa tagtgtttct taatttattc ggaagtttgg aatatttgtt
    22561 tgtagttttc cctttcttcg agtaaaataa ggttgtagaa ggtaagctgg aattaccttg
    22621 cgggaaagag gtgtagaatc atgggtttta cagatgaaac cgtacgtttt ggttttgacg
    22681 atagtcgcaa aaaagaagtg agcgaaacat tagcgattgt ttatcgggct ttggaagaaa
    22741 aaggctacaa tccaatcaat cagattgtcg gctacttgct ttctggagac ccggcctaca
    22801 ttcctcgtta tcgtgatgct cgaaatttga ttcgccgtca cgaacgcgat gagatcatgg
    22861 aagtaattgt caaggactac ctctcaaatc atggagtgaa tttatgagag taatgggctt
    22921 ggatgtcggt tctaagacag taggtgttgc tatcagtgac ccctttggct ggacagctca
    22981 aggagtagag atcattcgga tcaatgaagc agaagaagag tttggctttg atcggttgaa
    23041 ggaattagta gcacaatatg aagtagaccg gtttgtagtt ggattaccta aaaatatgaa
    23101 caattccatc ggaccaaggg cagaagcttc catgtcatac ggagaaaaga tcaaagaact
    23161 attctccttg ccagttgatt atcaagatga acgattaacc actgttcagg cagaacgtat
    23221 gctagtagag caggcaaata cttcaagagc caaacgaaaa aaagtcatcg ataaattagc
    23281 agctgtgatg atcttacaaa attatttaga cggctcaggt aagcaattat tttaataata
    23341 aaagaaaaga ggtaatctta tgacagaaca taaccattcc cacgaccatg aaggacacga
    23401 acacatcaca ttggtggatg accaaggaaa tgaaacatta tatgaaatcc tactaacagt
    23461 tgacggacaa gaagaatttg gacgtaacta tgttctttta tatcctgctg gtgtttcaga
    23521 agatgaagat gtagaattac aagcttatgc atacatcgaa aacgaagatg gaacagaagg
    23581 cgagttagaa caaatcgaaa cagacgcaga atgggacatg attgaagaag tattcaatac
    23641 attcatggcg gaagaagaag aatagaacaa aagaatgaat taataagata taaaaaaagg
    23701 gattgtgaca aacgtcttgt cacaatccct ttttccaaat aaacgatgtt caatcagctg
    23761 cccctcggca ttcgctcgaa aaacggcgaa atatgaagaa ctattttgcc atttgtttct
    23821 ctaataatca ggtcaaaacg ttgacttcac agtctcttcc ttaaacggtg ttctcctgcc
    23881 aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgtttt ttgaaattgt
    23941 tccttgtgac tcggagctga acggcaggct cacgacctct ttcttaaacg gtgttcaaaa
    24001 gcttgcatct gcggtaataa gttcaaccaa acgaaaatta tgaggagcta tttttgtttg
    24061 gctgttctta ttacttgatg caggacagct ttttcacaac ctcttttcgt ttagcactaa
    24121 atttccttaa aaagtagaaa agctttcgaa gcctcgtttg tttcttttac tgtataataa
    24181 aagaaaagag taacaatcgc taaaagatat aaacaagaga aaggaaatac aagatgattg
    24241 gtctgattta ttttgtggtg atcgttctgg cgaacacagt tggcgctatt tccgggatgg
    24301 gtggaggcgt gttgatcaaa ccgattcttg atcttatcgg cgcccattct gttgcaggga
    24361 tttcttttta ctcaacagtt gcggtattca cgatgtccat tgtttcgact gtgcgacaag
    24421 tatcaagcgg gaaatcactg aattggcaga ttgtcggttg ggtatctagt ggagctgttg
    24481 ttggaggagt agtcggcaac attgtattcg aagttttttt acagttattt gaaaacgaga
    24541 agcatgtgca gctgatccaa atttttttga cggtattgac cttggttttt gcgttcttct
    24601 acaccaagca tcatcagcca aaatttcatt tgacgtcatg gacttggtac cttatatgtg
    24661 gggggggttc tgggcttttt agctagtttt ttaggtattg gtggtggacc agtcaatgtt
    24721 tctttgttga tgctgatgtt tgcattgcca ataaaagaag caacactata ttctctatct
    24781 acgattttct tttctcaact ggcaaaattg gtaacgattg ctttaacaag tagctttatg
    24841 cgctttgatt tatctatgtt gttttatgtt atccccgctg ctgttattgg aggtttgtgg
    24901 ggagcaagat ttagcagggt tttgtctcca aaaaaagtga cctttatttt tcaagtaatt
    24961 gtcctcgtgg ttcttttggt caattgttac aatgcctttg tcatcttaca aacattagga
    25021 gaatctagac taacgcaagt caaaatcact gaaacatatt gttctaaatg aaaaaaagag
    25081 tatactatga taaaaaaatt cattttaaaa aatcagaaat tggctggtga tttggatgtt
    25141 acgggaaatg cgtttagaag atgcagaagc actacgtgaa atcaatgcgg atcaattagg
    25201 gtatgatata ccattaaccg ttacggtgag acaaatgaag aaactgctga aggagccaga
    25261 gaaaaatttt ttcttggttt atgaagatcc tacttctaaa aaagcagctg gctatgtaca
    25321 agcggagtcc tatgaatcga tttttagcga tccaatgttc aatattatgg cacttgctgt
    25381 gtctaaatat gctgaaaaaa gaggcatcgg aaaggctttg atgactggcg tagaagaaga
    25441 agcgaaaaaa cgaggaataa cgacaataag gcttaattct gcagaataca gagtagaagc
    25501 acaccaattt tatgaacaca tcggttatca ttccgataaa atgcaaaaaa gatttttgaa
    25561 aatcctgtaa atctttatca aaaaaatacc cgatcaccaa gccgatactt gatgatcagg
    25621 tatttttgtt tttttgacta agaaagtaat tgcatcaaga aaccagagat gatcaaaacg
    25681 atggcaccac caagtcgatt gcccatttga gcaaaagcaa taagttccat ccgattagct
    25741 gcagataaga cggctacatt tcctgttccg cccatcgagt tgttgcataa gccagcagta
    25801 atagttgatt caattggata taaaccaaac aaacgaccga caaatcctga aacaactgaa
    25861 atcgcgataa cacttgtcag acataagaca acaaattgcc atgtcaaagc agaagctaat
    25921 acattcagat tcagcaacgc aataccgata ccggcaagaa cagctgctgt caagtttttg
    25981 acaatcaagt tattgaacat gatagctgca tcttcataat actctggtaa taggttggcg
    26041 attttacaga tgacaacgat aataatcata aatgcgtaag catggacttt aggaacaaaa
    26101 tagttaccaa tttgtcctaa gatgaagaaa gtcaatgata ccatcaaacc aacacctaac
    26161 tgtggaatgg aaggtttcac agttcgaggt tttccatcgc caagatcgcc tttttccatt
    26221 aatttaccat tgccgttgaa ttttggcatt gattgtccag cacgagcaac taatgctgca
    26281 ccgatgatag ctaagacatt tgtcattgct gtcgcaggaa tcaggcggga aatgatagaa
    26341 gaagcatccg tacctaaaac atttgcataa atcgttgata aaggaacggc tccggctcca
    26401 acaccaccag ccatcgcagg taatgagatg tataaaacgg aatttgcaaa accatttccg
    26461 ataagcatgc ccacgagtcc tacggcaaaa aaggagacga ccattgaaat aagtgcaacg
    26521 ggaataaaac gaacagaagc tttcatcaat agattacggt tcataccaag gatgctacca
    26581 gtaatcaagg cagcaatata aaagtcccaa aagcccatat tgctgacaaa gttttctgtg
    26641 gatttcacta catattcagg taagatccca aatgtagcaa tcgcagcaga agcgaagatc
    26701 gcaaaaacgg aaccaccacc caaataactt ttgacgatag ggatttttgt cccgataaaa
    26761 tgaaataaat taccgaaaat aaccaaaact gcgattggtc caatcatatt cgtcggtaat
    26821 ttccctaaag ccattgcaat gatcaggaca aggaccatga tcaaataaag tggcaaactt
    26881 acgccggtga tttttgtgga ccaaaaacct tcttttttct tttcctcaac tgcagttgca
    26941 gttgttgttt tttgtgccat ttttacagat cctttcttat tctgtatcat atgaaaaaag
    27001 ccaaagaaaa gcgacaaaag agcaggagtt ggctggccgc tgttttgccg cagtcgcttt
    27061 tttcttttta gacgtcatac aaagtttctg tatgttcttt tactataacg atcttaatct
    27121 tctaaagatt tatattcagg aacccattta gcagattcaa ctgcttcttt gatatccgtg
    27181 atttcttcac gattcaagtt ttgtgcgaca actgattttg cgacagtttc agcaatcgtt
    27241 tgagaaaatt cagtgatttt tgctactgga ggcaagatag ctgctcctgg ttttgttaca
    27301 tcaacgattc cgcctaatgc tcgactagct tgagaaatga tttcagcatt tactcgtgta
    27361 gctgttgaag caatcaaacc aagtcctaaa cctgggtaca tcaaagcatt atttgcttga
    27421 ccgatttggt aagttacacc attatattca acatcagcag cagggatacc tgttccaact
    27481 aatgcttttc cgtccgtcca ttctattaaa tcttttgctt ttgcttctgc caattttgtt
    27541 ggattagaca aaggcatgat gatcggacgt ggtgtatgag cagccatttc tttcacgatt
    27601 tcttcagtga atgctccagg ttgggtagaa gtaccaatca tgattgtcgg atggatcgct
    27661 tttacaactg cttctaagtt tgtcaattca tcagcattag tgaaatctga gcgtttgcga
    27721 gcgaatggga tttgaccagg agtcaatcct tctgtatctt caaacaataa accttgttta
    27781 tctacttgat agaaatgttg gcgtgcttct tcttcaggta caccttgacg gatcatctca
    27841 tctaaaagga tgtttgcgat acccacacca gcagtaccag caccaaatgt caaaattatt
    27901 tgatctttca attgttcacc ggatatattc aatccgccta atacacctgc taggacaaca
    27961 attcctgtac cttggatatc gtcgttgaaa gtagtgattt gatcttcgta tttttctaag
    28021 atcgctgcag catttcctcg tccaaaatct tcccaatgca ataacaattc tgggaataat
    28081 tcggtagctg attgaacgaa ttgatcgatg aattcatagt aagtttcgcc ttctacacga
    28141 gcatgacgat tgcctaagta taagggatta ttcaataatt cttcattatt agtaccagcg
    28201 tcgatcgata caggcaaaac ttgtgctgga ttaataccag cagcagcagt atagaccatc
    28261 aatttgccga tagcgatgtc aacaccgttc acaccccagt cgcccatacc taagattcct
    28321 tctgcatcag tcacaacaat caaacgaatg tctcgtccat ctgccgcgtt cttcaaactt
    28381 gctttgatgt tttctggttc gtcgattgaa agaaatgcag catcttgcgg tttcaagaaa
    28441 atttcgttgt attgttcgat tgaatccgca acgacaggat cataaacgac aggcatgaat
    28501 tcaactaaat gttcgcccat caatttataa aacaatgtcc gattcgtatt gaatagattc
    28561 attaagaaga tacgtttttc caaatctgtt tgtttgctta agtattgacc ataagcttga
    28621 acagattgtt gttccagtgt ttggacagta cttggaagca taccagtgat tccatatttt
    28681 gcccgttctt cttttgtaaa agctgttcct ttatttagaa aaggattatt taataagttt
    28741 aaccctgtaa gcatgaatga ttcctcctaa gttttaatac aaacgaagta tagaagtcag
    28801 atgatgatat agtcaaaaag agcaggtatg ataaatttta tttatatgta atatagccaa
    28861 aagaaatcaa aatagcagta tttgttcttt ttatatcgac aatccttgct tgaatactat
    28921 gcgtatctct tatatgctag agaaagaaaa gtagaagaat aagggataga tggaatggat
    28981 aaataggatt tctgaggaaa aaagcacatg atagcgttga taggagtcac ctatgtcagg
    29041 tgataaaaat acataagtag aaaattaaga ggaggaagag aaaatgaaag aagttcaatt
    29101 gattagaaaa agcgaactat cagaaggcgg ttgcaatgca tgtggcgtag tagaagcgac
    29161 aagctataca ttaaaattag attccaatca agcaataatc tctgaactga cagtaggcgg
    29221 tcttgtggac agtctggcgc ttgctgaagg ttttactgga gaagatattt atgagatgtt
    29281 ttcagaagtc cgtcagttaa aaaaaggaga aaaatgcata gaggttcatc atgagagccc
    29341 gaatgtgagg ttcaaacgtg gtgataatga gatgatcttc aagaatcatg tgtctgatca
    29401 cactgaacta tatgaaatcg tgaatcaaat actgtctggg atttttgaat taggacctta
    29461 tgagttcaag gaagaaaacg gtaatccaaa attaaatgaa gaatggcaag aaacgattga
    29521 aacacaaaga aacaatccac acttatttca atagtcttcc acagtatttc gtaaaaatgt
    29581 tgatgaaaaa gaaacaccgt gaatcagagg tcgtgaaaaa cctgccctgc atcaagtaat
    29641 aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggttgggct tattaccgca
    29701 gatgcaagtt tttgaacacc gtttaaggaa gagactgtga agtcaacgtt ctgacctgag
    29761 tattagagag acaaatggca aaatagttcc tcatattttg ccattttttg agctaatacc
    29821 gagggtcagc tgattgaaca ccgttttatc gagaggttgt gaaaaagtcg cttagtttcg
    29881 agtagtaaga aaaattttgt gaaaatggct ttccacattt ttgcataaat ttgacttaat
    29941 accgagaaaa tggcttttga acaccgttta ttcaaaaaaa gggactttga cactactttt
    30001 gtcaaagtcc catcggtttt ttatctattt ttattacaat catttcttcc agagtagtga
    30061 catctggaat ggctgaaatg taaacagatt atttgcctgt atcaatgtcg aagagtaata
    30121 taaaaagtgt aaacgctatc atattgttca tcattcaaat tttatagaga gaaggcattg
    30181 agatggaaaa aacatatcaa gcaggtacaa atgaaggaat agtagatttt atcaatatgg
    30241 aagatttaga aatcgcggct tcacaagtga ttccagctgg tggttatggt tacatcagca
    30301 gtggtgctgg cgacttattt acttatcaag aaaatgaacg ggcattcaat catcgtttga
    30361 ttattcctca tgtattaaga gatgtagaat tacccgatac aactacacat tttgacgaag
    30421 aaatgctgac tgcaccaatc atcatggcac cagttgctgc tcatggatta gctcatgtga
    30481 aggcggagaa agcttctgca aaaggtgtag cagattttgg gacgatctat acagcaagtt
    30541 cttacgcttc ttgtacttta gaagagataa gagaagcagg cggggagaaa gcaccgcaat
    30601 ggtttcaatt ttatatgagc aaagataatg gaatcaattt ggatattctt gaagtggcca
    30661 agcgtaatgg agcaaaagca atcgtattga cagcggatgc aacagttggc ggaaaccgtg
    30721 aaacagaccg cagaaacggg tttaccttcc cgctgccaat gccgatcgtt caggcttatc
    30781 aatcaggagt aggccaaacg atggatgctg tatataaatc ttccaaacaa aaacttagtc
    30841 caaaagatgt agaatttatc gctgctcatt ctgatttgcc agtctacgtg aaaggtgttc
    30901 aatcagaaga agatgtctat cgatcattag aatctggtgc tggtggaatt tgggtatcta
    30961 accatggtgg ccgtcaatta gatggggggc ctgctgcgtt tgattcgttg caatatgtgg
    31021 cagaagcagt agataaacgt gtaccaatcg tgtttgacag cggtgtacga cgtggacaac
    31081 atgttttcaa agcaatcgca tcaggtgccg atcttgttgc tattggcaga cccgttattt
    31141 atggattgtc tttaggagga agtacaggag tccatcaagt gtttgatttc tttaaaacag
    31201 aattggaaat ggtcatgcaa ttagctggaa cacaaacagt agaagatatc aaaaaaataa
    31261 aattgcgtga gaatcgtttt atttaagaaa taaatgacga aaaaaatagg agtgcggcag
    31321 atgatgccgt actcctattt ttatagttca aggatgtcac taatagaata atttgaaacg
    31381 aaaaaataaa attgaaatat tttttcatac ttaataaaaa aggaagaata ttcatgtaaa
    31441 cgattttatt gtttttttct cttttataca aaaagggtca taatttcaag taagcggatg
    31501 aattcatgag gattttttgc atactgtctc tactatactt aggtcactaa gaaaaaggag
    31561 tgatttatta tgaaaaagaa catgattggg acattattac tttcaacact tcttctaggc
    31621 ggcttggcaa caccagcttt tgctgagggt caagcgactt caaaaggaga tattaccttt
    31681 actgaaccaa caaataccgt tgagcctctt aaccctactg atccttcaaa accagtagag
    31741 ccagcagatc cggaaaatcc agcaactggc cagacaggtt ctttaacatt ggatgtagtt
    31801 cctgaattgc catttggtac acacgagatt gaaagcggaa caaaaacgta tcaagtagac
    31861 gcttcaaaaa acgatacacc ttatctccaa gtatcagatc gtcgaggtgt aggtgcagat
    31921 ggccaagcac aagggtggaa cgtgacagtt tcggtttcag attttgtaaa cggttcacaa
    31981 gttttgcaag gcgctgaatt agattttgga acatccactg ttaaaagtac gtcagataac
    32041 gaatccactg gaccaactag tcaaactgta actgggttga gtaaagcaag tgcagcgaca
    32101 ccaatattta cagcagcaaa agatcaaggt ctgggaacat ggctttctgt ttatgatcca
    32161 gcaaatatca cactgaaagt gccgaaagca gcagctggaa cattcacggc tgatttgact
    32221 tggaacctcg tggctggccc agtagcatag agtatgaaat aaattcaaca aactgcggtg
    32281 aaaatactat aggtgaagga ttttttaccg cagttgttgt tgaaaggatg tggtggacaa
    32341 tgaatcaaag taaacgactg tttgtttcgt ttttttctat tttaagtcta tttttcattt
    32401 ttccgtcaat cagccaagca aaggattcag caggagactt tggaatcaaa ccggtatttc
    32461 cagagaacca aatagataaa gcaatcggtt attttgattt attagtggca ccggaacaaa
    32521 atcagacact agaagtaatt atcagcaatt cttcagatga agaacgaaca tttgaggtct
    32581 ctgtcaatcc ggcggtaacg agtgatggtg gaacgatcga ttatagtcaa aaaaacccaa
    32641 cgttagatga gacattgccg tttgatgtcc gtgacgtttt gctcattgcg aaaaaggaaa
    32701 tcaatgtatc agcacatgca gaaacaacta tacctataga agtgaaaatc ccagcaaaaa
    32761 gctttaaggg acgtgtatta gcgggtattc atgtttcacc taaagaagaa gctgaaacag
    32821 aaaatgctac agaaggagca caaatcaaaa atcgtattgc ttataatctc gcagtcgttt
    32881 tacaagaaag tcaagagacg atagaaccgg atctgaaact tttatcagga gatttggacg
    32941 aggtcaacgc aaagccaact gttcagcttc gtttccaaaa tcctcagcct aggattatca
    33001 gcaatttgat atttacgagc aagatctttt atgaaaatca gctttatatt gaaaatacgt
    33061 ctaatgaatt tttagttgca ccaaattcga attttcactt gaatcttgat ttagctggtg
    33121 ataaagcaaa agccggcgat taccgcgcag aaatcatagc aaaatcaggc gacagtaatg
    33181 aatggcgatt tacacaaaac tttacgatca aaaaagaaaa agcacaaaaa gtaaatgaaa
    33241 attccgtttt tgcagtacaa gaacaatctt tcccatggct ttatgcagga ttggttgctg
    33301 cagttctagt gttatggttg attttgctgt ttttgtgggt ccacaagaaa aagaaaaaag
    33361 gagaaacaac atgatgaggt gatttcgttg aaaacatcga tcttattggg agggattatt
    33421 gcaagtttgc tgttcttcag ctggcaagag ccagtccaag cagataccaa aagttcaggt
    33481 acgattacat tttatgatct aagtccagtt ccgcatccaa gtggttcagt caatggcgca
    33541 tcaaaacagc agacacagct accgatttat aaagaatcta atcaacatgg cgtattcccg
    33601 gagacgaatg aagaaaagat ccgttttctt tatttgatca ttggtataga attatggctt
    33661 cttgcgtggt tgttgttatg gagaagaaag gaaaaggagg atgaacatgt tgctacaaaa
    33721 taaattttca ttttttcgct ccttcttttt tacttcgctt tttgttttaa gtatatttat
    33781 cttttcacct tcggcagaag ctgttggagc aacgcctcct gccatttctt atgatccata
    33841 cacgtatgaa aatatcgtac ctccaaatgg tggacgtagt gcagtcaatg gcgggaagct
    33901 tgtccaacta actggggcta accaattgcc taaattgact gctacttatt tgaacgatgg
    33961 ttcaaatgat ccttggaaag gtggcacggt ccgttttcat tgggaaagcc atgtgaaact
    34021 aacaaatgta cttgatttgg tgaatatcac tgcacaagga tttgcatttg tggcgatcat
    34081 caatttgccg gaacatgtat caagcagtga tgtattaaaa gcaattgact ggaacaacgc
    34141 ctacttgtat attgccggtg cccccatcaa ggttactagc ggtagtttat tatcagcagg
    34201 aagccatacc ttgcgttttg ggatggggtc gtgggataca aatactctat tggcagatat
    34261 tattgctatt attacgcgag gcgaattcaa cgtggacgat atcccgcttc aatttgatgt
    34321 tacagctaat gtcgcgaaga tgacagaaaa cggagataaa tatgatacga gtccaaacaa
    34381 aattttgacc aagggtaaat tacaaccttc tatgtctaaa aaggccgttt tttctgttga
    34441 tttttatgat tcgaataata ttatccaaga tcgtatgttc aattcgattt tagctccagc
    34501 tgcaggtaga ttttatccta aattaactgg cggaaacttg aatccaggag catatgcatt
    34561 ttctgcaagt gcagagataa atacgtggaa tagttacatc tcaacaagtg attcaacagg
    34621 aaaatataat acatttgctg ataccacaga atacgtggat ggaaataacc agaaccttcc
    34681 aggctctacc aatagtcgga cattgacgat ggggctgact gaaggacgtt ttgatcagtt
    34741 gccatttagt cgctttgatc gcgtagtcaa ctattttaca ggaaaaaacg ttacacaagg
    34801 agcaatactg actcatactc cagcaagcac aagtggattg aatcagacag ccaatatcgt
    34861 gtacagtggg aaagatgcgc aaggcggatt gctttctcct gttgctttaa aggtattgga
    34921 gcagtacaag ctagacggaa cggttttacc aacaaactta acagccgata gtcagtgggg
    34981 aaagctcctt tcttctgcat cgtaccaagt cacggccaat tggtcagcaa ctgaactaaa
    35041 aaagggttcg atccattaca ggctttattc gaaaggaacc caaaaattag cgacaggatg
    35101 gacagatcaa cttttccaaa cgatccaatc aaataacggg ggtgtaaata cagctacttc
    35161 tacactacct gcattggctt cagggcaata ttattttgat tatcgcttgg tggacgatgt
    35221 acttactaac atgtatccgt cttttgcgta caaatggcag tctgaacaag ctggaatcac
    35281 cactcttcct caaataacag tggctaattt tccatctgtg agtgcagata gtcagctaaa
    35341 aaaccttagc agacaacctg agacaggaga tcccctgtct gctctatcag gagatacgat
    35401 tcaagaaaat gtgacattca cgttaacaaa atctggggat gcgataaccg acaaaaaaat
    35461 cacgatctca cttcctgaaa atactactta tatcaatggt tccttaaaat taaatggtac
    35521 aactatcgca gataccggca ttcaacaagg agtttctgtc ccagcagatc tattgagcaa
    35581 aataggagat accattcatc ttacttacaa ctatcaatta aatactgtag atacttctgt
    35641 acaatccgtc agcatactga caaaggcagc tgtactttcc agcaatatta cattagcaga
    35701 tggtgctaag cttcctaacc cagttgtcca aacatctgct aaaacaattc ttgtccccaa
    35761 acaagaatta acattagtta atgtaccaga tgattttaca tttggtaacg atctccccaa
    35821 accgttgaag acaagttatt atgaagcaaa aggcgatttt tcttttgatg tgcgggatac
    35881 tcggcttcca agcacgtctc cttggcaact gacagggaca ttgacttctt tatttaagaa
    35941 tgatcaaggt caagaattaa gcggaacaaa attatacttc aatcattctg gatcaaaaca
    36001 attgattcag caaggtcaaa acacgttgat ttatgagagt gatggaacag ctaaaggaga
    36061 ggttctcgtt gactttcccg ataccgatgg tttactctta gaggtaaata gcagtacgaa
    36121 tgctcaacct ggcgcaacgt atcaaggcat ggtcacatgg gaactgacag ctgggccaac
    36181 cagctaagtg attagaattt gtatggatct gtgataactg tcacagatcc tctttttcaa
    36241 ggatgagaag gaaacttctg ctgttaaaat cagaaatgct tggaggagtg gacttggaac
    36301 ttttcagttt gcttgaaaaa aatgaacaac ggcaaatcaa acttttccaa cttttattag
    36361 aggaaaaagg agagatttcc gttaataacg tacttacccg cttatcagtt gatcgggcta
    36421 ccttgaaaga agatctgttt tatttacaag ctaacttgga aaatatcaaa caattggaaa
    36481 ttgttatcca agaagggaat attttattaa tcagacatgg aaatatagca gcagtcgatg
    36541 tttattatca ttgctacttg cgctattcta caaaatatca gatacttctt tatctgttgg
    36601 aaaatggctc atttgatcgg cagaaacttg tcatggaact taatatcagt tcggctactt
    36661 tgtctcgcag agtgaaggaa ttaaatgatt ctttaaatga gtttcatctg aaaataaaaa
    36721 gcgggcggct gaccggtcca gaaagtcaaa tttgttattt ttatttccaa ttattttggt
    36781 ttggacgtcc ttatcttgaa aacaaaaaag aatacagtga tttatcagga aacacttttt
    36841 tagatctgct gcaaaaagag tttagtttta ctattacaga agaaggacga ttgaaatttg
    36901 aaatatggct ttccattatg aagaagcgaa tgcgctgtac gaaaaaatcc aaaccagatt
    36961 atagcaatct tcctgttgga ttgtttcaaa cgacacaatt tttattggac ctccatacat
    37021 ttttgacccg ttacttcttt caatatgcct ttccttggaa tcaaaatgaa acgttggttt
    37081 tttatctttt catgatcagt aactttacat tagatacaca taactcgcat gtccaggagt
    37141 ttttgataag cgaaaaatac aaagacgacg tagtagctca attgaatcga acgtttaaac
    37201 aatcgataga agaatatttc tctttttatg tttttccaga agagatttcg caaaagattg
    37261 cgttgacagt gatgcaaaat cacgctcatt ttgcctattt tagaggatgg atgaatattt
    37321 ttggccaaga aggattaaaa cagcggttaa ctgaagtaga agataaacaa ttcctggttt
    37381 tatgtgaaag attggtcaga caaacatttg ctattgttga ttttactgaa gtagacgagg
    37441 aaaatgcccg ctttgaatta tttgctcgct atgcatctat tttgcggacg ctgatcaagc
    37501 agttagaatt acgtctgcaa attgcttatg atcttcctta tgaaaggcta atggcggata
    37561 tcgtgaaaga aatcatccag gaacaattgc tgaatacgat caaatatgat ttagtcgaat
    37621 acgagaaaga aaagcagtat gatatcattt tgactagcca attgaaagag tacccgtcac
    37681 agaagtccgc taaagttttt gtattcacta gcaacgaata taattatgat tgcccctatt
    37741 tgaaccaatt tttaaaagaa tgttatttag aaaaattgaa tttaaggatg aataaaacat
    37801 gaaaatcgtg atcattggtg cttcccatgc aggcatcaca gctgccctaa atctaagaaa
    37861 acttcaaccg aaagcagaag ttttactgat cgatgaagat cataaagatg gactaggcta
    37921 tgtttcaaat ggaatcaacc tttatttaaa aggaaagata cgtagtttag ctgaagttgc
    37981 ccataacatg cggactcttc aagaaagtgg ggcaaaactg atcaccgaat ggagagtaac
    38041 tgaacttgat ccagacaaac atcagctcat cctcgcatcc aaagaaggaa aaaacgagac
    38101 cataacctat gataaattaa tcgtagcaac aggttcctct cctgtcacct tgtataaaca
    38161 aattgaagct gaaaatgtct acacctataa aaatctagtg caatctaagc aagtacttgc
    38221 cgctttaaaa gaagcaaaag aagtagtgat cttcggtgct ggttatattg gattggaatt
    38281 agcagatgct ttgagaaata aaggatacat tattcatcta gttgattata tgccgaatgt
    38341 tttatcaaga tatttcgata aggatatgat caacagtttt caaaatcagc tgcaaactaa
    38401 gcaaatcaat ttttatccaa atgaattctt gatcgattgg aaaaaatcag aagagaaagt
    38461 agtatccgta caacttctat ctcaagcaat aaaggcagat atggtcattt tttctgccca
    38521 aactcgtcct aatacgacat tgttaaaaga aaaagtggct ttgtatgagg atgagactgt
    38581 gatagtcaat gaatatctgc agacaagtga tccggacatc tatgcgattg gagatatcgt
    38641 cccagtcagt tttgacaaaa ataaacggca tctcttttta cctttagtta ctcgagctgt
    38701 acatatggca agagcagtag cgctaacttt gagtggtcag ccaaccgctt acgacctcag
    38761 acaaaagata acagcagcag tcattataga ctacttttta ggaactgtag ggctgacaga
    38821 agatgaagcg ccattcttgg aacaaaatac cggttcgtgt tcaggagaat ttgatctatt
    38881 tccacaatac gatgaagaaa acaaaacagt aaatgcaaag ctgatctacc atccggatac
    38941 attagaaata attggcggac agctgataag ccaagaattc ttattatcag atttgaacct
    39001 gcttgcggat atcgtgaaac acaaaactac gattccacag ctagcagttg aggattttgg
    39061 atttctgtct gaacacacct ctcgttttca ctacctcaat gagttagctt ttaaagtact
    39121 agctaagaga gcaaatagca atagagtaga taaaagactg tgagaaatat cacggtcttt
    39181 tattgtgtgg ggatataata aatattaatc aatcgagaat aaaaaattca tataatgaaa
    39241 tagcggaaga attttttgag cataatattc gccaaaaatt tatcaagtga taaaattgtt
    39301 gtataaaatg attatttcag gaggaggagt taaatgtttt tagcattaaa tgaaattatg
    39361 cattcaaagt tacgttatgc gttagttgca ggggtgatgt ttttgatcgc ctacctggta
    39421 ttttttttga ctgggttagc ttatggctta gctcaagata atcgaacagc agtcgacaag
    39481 tgggaggcag attcgattgt gctttcgaaa gatgcaaatt caaatctagg gatgtccatg
    39541 attactaaga agatagcaga agaagtcgaa ggtgggaaag tggcgtatct tgcccagaca
    39601 cctggagtcg tgactagtaa agacagtaca gaagaaggaa aaatcaatgt tagcttcttc
    39661 ggtatcgata aaaatcaatt catcatgccc aatcttgttg aaggaaaagc ttttgataac
    39721 gatgatgagg cagtaggcga tattagtttg aaagaagaat atggtctagc cgtaggagat
    39781 acagttaagt tatctggtag tgacaagacg tttaaactaa ctggctttac ggatcatgcc
    39841 aaattcaatg tctcaccagt tctctataca accatcaatg cttaccaaga gatccgcttc
    39901 gaaaaagaag atacaagtga aaatgcacgg attaacgcaa tagttgtcag aggtaaaatc
    39961 agtaatttac ctgaagattt agaacagatc aaaatttcta aattcatcaa tgaattacct
    40021 ggatataatg cgcaagtatt gacttttgga tttatgatcg gatttttgat cgttattgcg
    40081 gctattgtga tcgggatttt catttatgta ctgacgatgc aaaagattaa tatctttggt
    40141 gtaatgaaag cacaagggat cacaggagga tttattgcgc gttctgtagt ggcacaaaca
    40201 ttcattttat catttgttgg tattcttctt ggtctattag gaacagtggg aacatccctt
    40261 gttctacctg atgcggttcc attccaaagc aattggctgt tttttggcgt cattagcttg
    40321 ttgatgctgg tagtagctgt gttaggcgca ctattttctg ttcggacaat cgtaaaaatc
    40381 gatccgttga aggcaattgg ataagaagga ggaagagcat gaaagcaata gaatttaaag
    40441 aagtagaaaa aagtttccaa gatggcgacc aagtagttaa agcattgaaa gaaacaacca
    40501 tgagtatcga taaaggggaa tttgttgcaa tcatcggtcc ttctggttca ggtaaaagta
    40561 cttttttgac tttggcaggc ggattgcaga cgcctagcag tggctctgta tttatcaatg
    40621 atcaagaatt cagtaatcaa aaagaaagtg aacgcgttaa actacgtttt aaagaaattg
    40681 gctttatttt gcaagcttcc aatctggttc cttttctgac agtaaaaaag cagttattat
    40741 tagtagataa gatcaaacat gaacatcgtc aaaaagaagc aaaagaatta tttgagcagc
    40801 ttggcgtgga taagttgatc aacaagtatc cagaagaact atctggtgga gaacggcaaa
    40861 gggtcgcaat cgctcgtgca ttgtacaatg atccgtccat tattctagca gatgaaccaa
    40921 ctgctagttt ggattcagaa aaggcacgag aagtagtcga tatcttggca aaagaatcaa
    40981 aagacaaaaa taaagcaacg atcatggtta ctcacgatac acggctgatt gacaaatgcg
    41041 atagaatatt tatgatggaa gatggtgtat tgaaagaaaa agaataacca aaaagcaggt
    41101 ggaaaaatgg tccatcgata caacaaagat acacagcaaa aaattttgaa agaagcgact
    41161 gacttgttta tgtccaaggg atatctcggc acttcaacaa gagagatcgc acaaaaagtt
    41221 gggatcaccc aacccaatct ctatcattat ttcggtgata aagaaaaact atatacggca
    41281 gttttagaaa accacttaaa agatgttgga aaagctttga gagaaatcgt tcaaacaagc
    41341 gaaacgggat ttcaagtaac cttgacaaaa atggcgcagt ttttgattga gacacatctg
    41401 gtcgatttgt ttctgatgct tcacgatctt gaaagcaatc tttcaaagga aacaagagat
    41461 catcttttct ttctatggaa gaaaaattat cgtgaacctt ttgaagaaat cttttcagca
    41521 aaccaatctg tgctaagaaa tgggatttca aaagaaatcg cagcaaggca ttttttctta
    41581 gtgttagcac cttacatcac tcggtcgaaa agacctgtgg aagaaccgct aaatgtggcg
    41641 caattgatcg atttatattt gaatggtgta ttaggagaag cgtaaagctg ttatataggg
    41701 agaaaaatag ttaggtgaag aagcaagata tgacgaagat cgttatatct tgtttttttt
    41761 atataggtag tttttcataa atacaaattt gttattctct catgattatc agggtatatt
    41821 tttatttatt atgtcggtgt tccaggaagt gttttaattt aataacgaag aaattaattg
    41881 tttgtagaat gaaggaaact atttattggg tagatgaatc accacgaata gtcaaaagga
    41941 gatttcagga tatgccaaaa atgaaaaaaa tcattttagc agtaatgtta ttttctacgg
    42001 ttatatcttt tacagcccct tttttgcgta cttaagctga ttttaagttt tgacccttag
    42061 tctggatcta cgaggtgaga gtcaagaatg aaaaaaataa ttggagcttt tgtatttgca
    42121 atcattatca ttattatctg ttttcaaata gatgtttttg gtcaaaaaaa gatagttgga
    42181 caaacggtca gcagtacgaa cagtacggtt acaaagacaa aggaatcatt tgatgataca
    42241 gaacaagaga cattaatcaa aatcggaaaa aatgaccgta cactatatac agatgactcg
    42301 ctaactaccc cacttgcaac tataaacggc ggagagctgg cggagtttct ttctgaaaca
    42361 aaaaatgctt atcagatcaa gacaaatgat ggctatactg gatatttagc cctcgaagat
    42421 ggaaccaaag tcacaaagaa tatacaaaca aaaccggaag agctatctga tgcagtgatt
    42481 gtattagatc ctgggcatgg cggaaatgat actggggcat taagcaatga tgaacaaaca
    42541 gaagaaaaag atataacatt aagtacagcc ataaaagtaa aaaaggcgct agaggatgct
    42601 ggagcaacgg tttatttgac ccatactaca gatgaattag tacagcttgg agatatatgc
    42661 gactacagtg aagaaaaaaa ggcagatgtc tttatcagtt tacacgcaga ctctacggaa
    42721 tatgcgaatg aagcaactgg aatcacgaca tattattact atggacaaga ggaaacatta
    42781 gcacagacaa ttgctgactc cttcactgat ctgcctctgg attcaagagg aatctccact
    42841 gggaactatc aagttcttcg agaaaatctt caaccttcta ttttaatcga gatgggttat
    42901 atgaataatg atagtgattt aaaggaatta gtaacagaca gttatcagca gcagattgct
    42961 gcaagtttga cacaagcatt gaccacttat tttcagtaat aaataagccc agccaaacaa
    43021 aaatagctcc tcatattttt cgtttggccg ggcttattat cgcagatgca agcttttgaa
    43081 caccgtttaa ggaagaggtt gtgagcctgc cgtttaactc cgcgtcacaa ggagcaattt
    43141 caaaaaacag cttctcatgt ttgatgacta ttgtgacttg tgaccgagga gttggcatga
    43201 gaacaccgtt tattcggaac aagggactgt gacatgactt ttgtcacagt tccattattt
    43261 ttttagcagc ttcaaagctt tttctgtatt ggcaaaatga agtttggcat atttttcaag
    43321 cataggcatg ccgcctttct ccaatagttt tgccgctact tggtcagatt tcgttccagc
    43381 tcctgatgga agggtgattc cagcttcagc tgatgctttg tgtaactctt ccaaaaagct
    43441 ggcacgactg atgatagaag cagcagcaac agctaaatga tattgttctc ctttagtaac
    43501 gaagtacagt ttttcttgta attgattttt ttctaaacgc gcatattttt taaaatttgc
    43561 ttccggcgta aactgatcga tcaaaatcgc ttctggtttt tctggctcga ttttttttag
    43621 cagcaaatgg atcgcttgat tgtgcaaagc gactttcatc cgaacggcat tgtattttgg
    43681 ttggatctca ttgtattttt ctggagataa gacaagcagt ttatagggga tggttttttt
    43741 taagatgtca gcaagctgga tgatttggct atcttttaac tcttttgaat ctctaacacc
    43801 aagtttacgt aattctgcaa gctgatcacg tttcacataa gcagcacata ctgttagcgg
    43861 accaaaatag ctgccatttc cgacctcatc actacctaaa acggataatt gactgatatt
    43921 ttttggcaaa gaagaggctg tttttgctgg cttggcttgt ttatcctgtg cctcccccca
    43981 tttatcagct tctattgctg cattcgtacc ttgaaacatc acttttccgg atgtataagc
    44041 ggtaatggtc gtcccgtctt tttttgcagc aaagctagta taaggaatct gtttatttaa
    44101 caaataagag cgatagtatg ctttcatttg actaagttgg tcgtttgttg ctttgataat
    44161 gacgttgctc ataaaattct cctcgttttt atacataaaa ctataccgca actcacgact
    44221 tttcgttttc taagagattg cgagacatag cattttttta aagtttatca tataatagtt
    44281 aagtatgtga aaaatatttg tggagaacgg tgaaagaacc aacttcatgg tacactaaat
    44341 aaaactttca tcaaatagga ggaaatgatt cgtatggcgc acgaaaaaac acgctacaaa
    44401 gcagtgattg ccaaccaaac atatactatt atcggacggg aaaccaagca tcatatggat
    44461 atcgtaacga agctgatcaa tgaacagtta gcagaattga aacaattatc accacaaatg
    44521 gataatgaac aagcagcaat cttgatggct gtcaatgcgc tttcggatca attgaagaaa
    44581 caagaaagaa tcttggagct ggaagaagaa acagcagaat taaagaaaaa aatgatcaaa
    44641 tttacagagc ttgaaaatcg tgtgaaacgc attgaagcaa tcgagaacga agctcgggaa
    44701 gtattgaaag aaaatggtca agcagatcat accattaaaa atcatgtgga agcacaacag
    44761 attttaaacg agaaacgaaa aggacaaatt aaacaaaaat cttctaatta agatcctttt
    44821 atcaaaataa gaaaaattta aaaaagttag aagagaaagg aacgtagcat gctgagtttg
    44881 ctgattttat ttttattgct aattgcgttt ttttctggag cacgtcgcgg gtttgctttg
    44941 caaatcatat tcgcgattgg atatgtattg tcatttattg ctgcccagca tttttacaaa
    45001 ccattagcaa gtcatttgga attatacatt ccatacccag ctgtcacccc aacatcaaag
    45061 ttagcatttt ttgatcaggt atttgctttt cacctagatg aagcttttta tgcaggaact
    45121 gcttttctga ttattttgct aattggctgg ctgcttaccc gatttgtggg ggtttttgtc
    45181 catggattga cgtatgtgcc gatcttgaga caggtagact ggattgcagg aggcatcctg
    45241 tcactaatca tggcttacgt tactatattt ttgattttgc agttacttgc ctttgttcca
    45301 ttagatgtgg tccaaaatca attcggcgag catagtttcg ctcgttttat cgttgagaga
    45361 acgccatttc ttaccaacaa gatgtatgag ctttggtaac gaatattttg aattaaggaa
    45421 aatagtgctg gaaaatattt cgttttttgt acagagtagt ttatttaaga aggctgcgta
    45481 gcgtatgtcc ggcacagctc ttttttcata gtgaaagata aggtgataaa atgaataacc
    45541 gaatccttga aacattggaa tttgaaaaag tcaaacagat ggtcagacaa tttgtagtga
    45601 ctgcacaagg aaaagaagaa ctggcagaat tggtaccagt gagtgagaaa caaacgatca
    45661 caaactggct tcaagaaact gaggatggat tgaaagtgca acgtctgcga ggcgggatcc
    45721 ctatacctaa acttgaaaat atccgtcccc atatgaaacg gatcgagatc ggagcagact
    45781 tgaatggagt ggagcttgca caagtttcca gagtattgtc tacgacaagc gaattgaatc
    45841 gtttcattga tgatctttct gacagtgaaa tcgaatttgc acgtttgtat atgtgggcag
    45901 atcagttagt cacgatccct gttttgagcc gccgtttaaa ggaagcaatc gatgaagacg
    45961 gacgcgtgac tgatgacgct tctcctgagt taaagagcat aagacagaac attcggcgaa
    46021 gtgaacaagc tgttcgtgaa cagttagatg ggatcgtccg tgggaaaaat gcaaagtatt
    46081 taagtgatgc gatcatcacc atgcgaaatg accgctacgt tatcccagtc aaacaagaat
    46141 accgcggtgt atttggcggt gtagtccacg atcaaagtgc ttcaggacag actttattta
    46201 ttgaaccaaa acaagtggtg gatttgaata atcgtttgcg tcaataccaa atcgctgaac
    46261 gaaatgagat tcaacgtatt ttaagtgaac tttctgctga acttgttccc catcgtcaag
    46321 aaatcatcca caatgcttat gtgattggta aaatggatct gatgaatgca aaagcacgat
    46381 ttggtaaaga agtaaaagcc atcgtgccgg gaatcagtga agacaatcat gtcgtcttga
    46441 aacaagcaag acatccactg atcgatcagg aaaaagtagt ttctaatgac atcacaatcg
    46501 gaaaagacta tcaagccatc gtcattacag gacctaacac aggtgggaaa acgatcacgt
    46561 tgaagacttt aggtctgtta caactgatgg ggcaagcagg attaccgatt cctgcaggtg
    46621 aagaaagtca aatcggtatt tttgaggaag tatttgcgga tataggagac gaacagtcga
    46681 ttgagcaaag tctatcgacc ttttcttctc atatgacgaa cactgtagat attttatcaa
    46741 aagtcaatga aaaaagtctg gtattatttg acgagctcgg tgcaggaaca gatccgcaag
    46801 aaggtgctgc tctagcaatc gcaatcttag atgatcttgg taaaaagtct gcttatgtga
    46861 tggcaacaac gcattatcct gaattgaaag tttatggata caatcgggca aatacgatta
    46921 atgccagcat ggagttcgat gtggatacac taagtccaac gtatcgtttg ctgattggcg
    46981 ttcctggacg aagcaatgcg tttgaaatct ctagccgatt aggtcttgat acagaagtta
    47041 ttgatgaagc gaagcagttg atgaatgatg agagccaaga tttaaacgaa atgatcacag
    47101 atttagagaa tcgccggaaa atggcagaga ccgaatatct ggaaatgcgt cattttgttt
    47161 ctgaggcaca agaacttcac gatgatttga aagaagctta cagctatttc tttgaagaac
    47221 gtgaaaaaga aatggaaaaa gcaaagaaaa aagcaaacga agtcgtgtca gaggcggaag
    47281 aaaaagcaga aaaaatcatt gctgatattc gtaaaatgca acagcagatt gggcaaggaa
    47341 atgtgaaaga gcatcagttg atcgatgcaa agacccaatt agcaaatcta catcaagaag
    47401 aaactttgaa gaaaaataaa gtattgaaga aggcgaaaga acaaaaaacg ttgaaaccgg
    47461 gagacgaagt gttggttacg acttacggcc aaagagggac gcttttacgt aaaaacggga
    47521 atcagtggca agtagaaatc ggtatattaa aaatgaatgt gtccgaagac gagctgacgc
    47581 ctgttgctcc tcaaaaagag ccgacacaac gagttatcca tgctgtacga tctgaatcta
    47641 gtagtcacgt gccgaatcaa ttggaccttc gaggaaaacg atacgaagaa gcgctaagtg
    47701 aggtagatca atatttggat tctgccattc tagctggtta tcctcaagtg actattgtcc
    47761 acggaaaagg aacaggcgca cttcgcaagg gaattactga ttatcttaaa aaccatcgga
    47821 gcgtcaaaag ctttgagttt gcaccggcaa accaaggcgg aaatggtgcg acaattgtta
    47881 aatttaagta aagcttacta aaggtaagca gatggttcgc tatccaagta tagaaatgct
    47941 atactaatca tagattttaa aaatggaggt ctttgatatg tcacaagtaa ttacagataa
    48001 gacatttaat gaagaaacag ataaaggact tgtattgatt gatttttggg caacttggtg
    48061 tggtccatgt cgtatgcaag caccgatttt ggatcagttg gaacaagaat atgatgaaga
    48121 agaattcaga attgctaaaa tggatgtgga tgaaaatcca gaaactcctc aacaattcgg
    48181 tatcatgagc attccaacat taatgttgaa aaaagatggt caagtagtag aaaaagctgt
    48241 tggtgttcat tcaaaagaac aactacgtca aatgatcgat caatatttat aaaaacttac
    48301 agcaaaaata agctgagggt gggactgaat ccagttccgt cctcggtttt tttgcggttg
    48361 agttaaggtt attcaaatta atattttttg agtataatta cgttgtcagg ctggattgtt
    48421 tttttgacag acatgctata ataactttaa ttgaatatga aggggagtac cagatgaacg
    48481 aacgaatcaa aaacaaatta gctttacttc ctgatcagcc cggctgttac ttgatgaaag
    48541 ataaaaatgg aacgatcatt tatgtcggca aagccaaaat attaaaaaat cgtgtccgct
    48601 catattttac tggcagccat aatacgaaaa cggaacggct tgttagtgaa atcgaagact
    48661 ttgaatacat cgttacagaa tcgaatatcg aagcattgct cttagagatc aatctgataa
    48721 agaaaaatga tcctaaatac aacattatgt taaaggatga taaaacctat cctttcctta
    48781 agataacgaa tgaaaaatat ccccgtttgg tgattactcg taaagtacta aaagacaaag
    48841 cactgtattt tggaccatat cccgatgtcg gtgcggcgaa tgagacaaag aagatcctcg
    48901 atcgattatt tcctctgaga aaatgcaaac ctagtcagac gaaggagcct tgtctttact
    48961 atcatttggg acaatgtctg tgtccttatt atttcgacgt agatccgaaa gtctatacga
    49021 aaatcgttga tgaagtgaaa aagtttttaa acggaggaca cgaaaaaatc gagtcagaga
    49081 tccatcagaa aatggaaaaa gctgctgaaa acatggagtt tgaaaaagct gcagaatacc
    49141 gagaccagat ccgtgccatt gagacaatca tgactcgcca aaaaatgaca aatactgatt
    49201 tactggatcg agatgtattt ggttatgcga ttgacaaagg atggatgtgt gtccaagtgt
    49261 ttttcgtaag acaaggaaag ctaattgaac gagatgtttc aatgtttcct ttctataacg
    49321 aagcagaaga agatttcttg acttacctcg gtcagtttta tcaggaaaat gagcatttca
    49381 ttcctaaaga agtcttgatt cctgatacga tcgacaagcc cagtgtagaa gctttgttag
    49441 ctacgaaagt gctgcaacca aaacgaggag aaaagaaaaa attagttgaa ttggcgaata
    49501 agaatgcctc agttgctttg aatgaacggt ttgatctgat tgccagaaaa caggaacgga
    49561 cgatcggagc agttgaacgt ttaggaaacg cgatgaatat cccgacccca atacgtatcg
    49621 agtcttttga taactctaat atcatgggga cgaatcctgt ggctgcgatg gtcgtattca
    49681 ttgatggaaa accggcaaga aaagaatatc ggaaatataa aataaaaaca gtcaacggac
    49741 cggatgatta tgcatcaatg agagaagtca tctatcgcag atattcacga gtgattcgcg
    49801 aagggttgcc tttacctgat ttgatcttga tcgatggagg aaaaggacaa gtagatgtgg
    49861 caaaagatgt cttagaaaat caattaggaa tcgatattcc tattgctggc atggcaaaaa
    49921 acgataaaca taaaacaaat gagttgctat tcggtataga tttggctgta gtaccgcttg
    49981 agcgaaattc accagagttt ttcttactcc agcgaatcca agatgaagtc catcgattcg
    50041 caatcacttt ccatcggcag ctacgcagta aaaacagttt tgcttcccga ttagatgaaa
    50101 ttgaagggtt aggaccaaaa cgaaaaaaaa tgctgcttaa agagttcaaa tcgttgaaaa
    50161 atatcacagc agccacgaca gaagaattac aagaaatcgg actgccgaaa aatgtcgcac
    50221 aaaacgtatt tgataaatta catcaggaat gaggaaaggg cactttacaa agaaagtaaa
    50281 gtgtcctatc attttatgta caacaaaaag agcgtttacg ttttgttggt aaataataaa
    50341 tcgcttacgt ctggtgtcta aggatatatg tgatttcttg aagaaggagt gaagaaatgt
    50401 tagaagtcaa ggatcttgtt aaaacttttg ggaactacac agctgtcgat catgtctctt
    50461 tccaaatccc cgatggaaaa atcatggggc taattggcca aaacggggct gggaagacaa
    50521 cgacttttcg tttgatcttg gattttttga cacaagacca aggagaagtg ttatggaatg
    50581 gccatcatct tggggagaaa gattatgatg tcatcggtta tcttccggag gaacggggac
    50641 tttatccgaa agtaacgatt caagaccaac tgctttattt tgcagaactt agagggaaat
    50701 caagaaaaga gattgaacca aaaatcgatt tttggatgga aaaatttcag gtaaaaggga
    50761 aaaagacgga taaagtaaaa tctttgtcta aaggaaatca gcaaaaagtc cagctgatcg
    50821 ccacgttaat ccatgaacct aaattgatca ttttggatga gccattcagc gggttagatc
    50881 cggtaaatgc tgaattgcta aaagatggaa tattggaatt gaaaaaacat ggttcatgtg
    50941 tcattttttc tagccacaat atggataatg tagaaaaaat ctgcgatcat ttgatcatgt
    51001 tgcgaaacgg aaaaatggtt ttgaatggaa aagtacatga gattcgtgaa tcatttggcc
    51061 gtacgaagtt attcttggaa tctggtttaa gccaacagga agtagaagaa atcgaaggcg
    51121 tgaaaaaagt gatccttcat gaagacaata cgttagagat taccttagaa gatcctgaag
    51181 ctgggaaaga aatctttact cgtgcgactc agttcggcta cattcctatg tttaaccagc
    51241 aaccgccaac tttggaagag attttcaaat tgaaagcagg tgaaatcgat gcgtaagttt
    51301 tggattatta ccaaagatgt ctatttaaaa aatgttaagt cgatttcttt cttgatcatg
    51361 atattagtcc cttttatcat gctgggtatc atttatgtag caggaaactt tgctcaacaa
    51421 aatagtgatg tcaataaaat cggcgtattg gctgataatg agcaaatcag tcagcaaatg
    51481 agccaagtca aaacagatga ttttgagttc aaagcagttg attctgaaga aaaagcacaa
    51541 aaagagcttt cggaagaaaa aatcgatgcg tatttagtag tctcaactga gaatgataag
    51601 ataaatggga tgctttatag cgaaaattca ttaggacaaa gtacacaact actgatacaa
    51661 caacaactga ctggcttgca atcgatgctt cgagcaagtc aattaggcat ttcaccagaa
    51721 gaagtagcta gcttgagcca acaagcaagt ttgactcgac aaaaagtcaa ttttgattca
    51781 aaaggaaaaa tgacagttgg agaagataac tcgacagtcc aatatgtcgt aagttatgta
    51841 gcaacgattg tcttgttcat tattattttg acgtatgcgc agatcattgc acaagagatc
    51901 gcttctgaaa aaggcacgcg gatcatggaa gtgatcctgt ccagtacgac tgcacaaaag
    51961 catttttacg gaaaactaac aggtgtttta cttgttgcat taacgcaaat ggcattatac
    52021 ggtattattt ttggtatagg atacagtcaa ttcaaagata tggaaatggt caaaaatata
    52081 ttagacggta tctcattgga taacttgatt ggaccattct tgtggtattc tcttctattc
    52141 atgatctttg gcattttgat tttttctgtt ttagcagctt tatgtggctc tttggtcaac
    52201 aaagcagaag atacagcaaa agcaattttg ccagtgactt atctatctct aggcggatat
    52261 atgcttggat tgatactagg agcttcagat ccaaacaata tcgttattcg gatcacttcg
    52321 tatattccat tcttatcttc atatattatg ccggttcgct tagcaaatga gacagtcggt
    52381 atgggtggag cattgatttc cttgttggta cttgtagccg taacattctt attgatgatc
    52441 ttgtctgcaa acatgtacaa atcgaatgta ctcgtgtata gcgaaggcgg attatggagt
    52501 tcattgaaac aatcgatttc aattatgaga aatgaacgaa aaaaagcata aaatatagaa
    52561 aaatggctct ttgtcaaatg atgttggtaa agagaaatct tagaataatt ggagatcaag
    52621 tggatcagtt cctcttgatc ttttttagtt gaaaatcaat gcgttgccat gacgaatcat
    52681 gatgcgcaat ttgaaattat aaaagctacg aaagccatat gcatttcgtt ttaacacttt
    52741 gatatggttg ttcagacatt caagtgggcc atttgaataa ctttgttgta aagcattttt
    52801 gatttgtggc agaaatttct ggaacgttct caaggtcgtc tgatagcctt cgggaagcat
    52861 gatattattg gtaagtagtt cttcaaacga tacgtagtct cgttccttta cagcgtaaag
    52921 aaagtcttga tataactgat agcctacacg caaagcaggg ctccctttca gtaagcgatc
    52981 cacaatatcg gtctctgtta aatgagcttt gaagccaggt cgccaaaggc gtcgagtata
    53041 atcaagcttg cgttcatctt tttgaagaag tttccagtat ctttttaatt gcttacctaa
    53101 ttgatagtgt ttttgttctt tgctttttaa tagttggttc gtttctttga ttcgatgatt
    53161 gcgaaaggta cgaccaatat gttggacgat gtgaaaccgg tcaatgatca gctgtgcttt
    53221 tggaaacagc tttttaacga gcgatacata aggagcgtac atatcaatca cgatccattc
    53281 gaccgcttca cgaatttctc gtgaaaaatg actaaaataa cgttttaaaa aaggcagttg
    53341 tcggttctcg acaatgtcca acaagcgctg tgtttgtcca tccatcatga tgaaactcat
    53401 agcacctgaa acaccccgaa ctgatttgaa ttcatcaaaa caaagtacct taggtaacgg
    53461 tgtccgaaaa ggattcaacg gacgataaaa tcgcttcata atacggtaaa cagaagagac
    53521 cgatagattt ttctgagcgg cgatctctgt cattgaggta ttttttttca gacgagtcac
    53581 gacagaccag cggactttct cactgatgaa acagtgtttt ttcgcaacag aagtatctgc
    53641 gacaaaggtc cgctgacaag ctttgcattt gaagcgttgc tttttcaatt cgaggtaggt
    53701 acggcattca gaaacatcgt tcattaacaa ccggacggtt gttgtacccc aacgcacaat
    53761 atctgaagca atctggtgtt tacagtggat gcattcttgg gctgtataaa tcaagtttcc
    53821 acgataaata tgacagattt gccctttgta tttttctttt gtgagacaat tttcgttaaa
    53881 agtaactgat aagtctagaa tatctaatgt ttctttgata aggtgagtgt aagacatctt
    53941 gattcctcct tttgggttgg ttcgccaaat taattctaaa ggatttcaag gtgtcttttc
    54001 tatatgtttt ggaaaataaa aaagatgctg gtgtcatcca ccaacatcaa aaagtataga
    54061 gctagaaaaa tatagaagga agctggacat ttattgttcc cagcttcttt tttgtataga
    54121 cctatttcta tttttttatt atattggata tatatattca ttgaaaatag agcataacaa
    54181 ctttataatg ttgagtatac caatttttgg aggcgtttta atggaccgct atttagattt
    54241 actttcagag aagttcccta caacggaaga tgtcatcacg gaaatcatca atctcgaagc
    54301 aattttgcag cttccaaaag gaacagagtt atttttgagc gatatacatg gcgaatttcc
    54361 tgcttttgat catattctgc gtatcggttc aggaaatctt aaagaaaaga tcaaagacct
    54421 tttctctgaa caaatgacgg aaacggagat cagccaattc actattttta cggcttatcc
    54481 agaatatgtg ttgacgactg attggtacaa aaaacaagat aaatcccagc tgatccatcg
    54541 tttgatcgat cttttacggt ttacgacagt caaatacact cgatcaaaag ttcgaaaagg
    54601 attgccgaaa gaatatagct atatcattga ggaactcttg tatatcgatg atcgtatcaa
    54661 cgggaaaaaa gactatgtaa aaaaaatcat tgaacaactt gtcttgatga aggaagaaga
    54721 aaaattttta acaaaacttg cgcagacgat ccaaaaatct gtcatcgatc atttgcatat
    54781 cgtgggtgat atttttgatc gaggaactca agcagccaaa gtaatggatc gaatcatgga
    54841 tttttcctct gtagatatcc agtgggggaa tcatgatatt ttgtggattg gtgcgtattg
    54901 cggctctgag gcatgtttat tgacgctttt acggattgcc gcacgctaca actatttatt
    54961 tgaattagag aaagaatatg gattaaattt acgtccatta ttttcttttg cccatcaaac
    55021 ctatcagaag aatccagtat ttacccctaa aaacaggaaa gatctatctg aaagagagca
    55081 agaagaatta gaaaagatcc atcaagcact ttctatcctc caattcaaat cagaacatca
    55141 gttgttgaat cgacgacctg aatttaaaat ggacgatcgt aaattgttgg aaaaaatcaa
    55201 ctatgaggaa tcaacgatcg ttttagaagg tcagctctat gagttgaaag ggacttgttt
    55261 tcaaacgatc cagccagatg atcctaaaaa attcttggaa gaggaacaaa aagtgatcga
    55321 tagtttgatg tattcattcc agcactcgtt aagaatgcaa aaacacatga cgtttttgat
    55381 cgaaaaaggg ggaatgtatc tcacgaacaa tcagcatgta ctgttccatg gctgtatacc
    55441 tgtagatgaa aaggggcagc tcttagcatt tgaattagac caatcttatc gcggaaaaga
    55501 actgctggga ttttttgaaa aacagataac tgaaagcgca aaagatttga ctgcaaatga
    55561 agatttggcg accgatttga tttggtatgc gtggagcggg ccattttcgc ctttattcgg
    55621 caagagtaaa atggcaactt ttgagcgcta ttttctcaca gagcctcata cgcacgtcga
    55681 aaaaagcaat ccctattatc acttgcgaga tgaagagtgg ttcagtgaaa aaattttggc
    55741 agaattcgat gcaaaagaag aaggttcagc aatcatcaat ggacacacgc cagtaaaagt
    55801 aaaaaaagga gagtcaccga tcaaagcaaa cggaaaagca tttgttattg acggaggtct
    55861 atccaaagct tatcaaaaaa caaccgggat tgctggctac tcattactat gcaattctta
    55921 cggtttccaa atcgtgacac attatccgtt cttgcctatt gaacaacaat ttgcaaaaaa
    55981 atctgatcaa acgaatgtaa aaaaagtgat cgagcagcag ctcccacgca aacttaatcg
    56041 gaatacgaca aaaggaatgg aattacagca acagattact caactccaaa ggctgttgga
    56101 ttatcgaaag gacgattgag aaaaatgcta gccaacattg ctcaatagac taaaaagcga
    56161 ctgcaacaaa aggcgtgtcg cagtctcctt cggaattagc tcaaaaaacg gtaaaatatt
    56221 agaagctatt tttctgttta tttctctaat actcaggtca gggcgctgat ttcacgacct
    56281 cttccttaaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata tccaacaaac
    56341 atgagaagct gttttttgaa attgctcctt gtgactcaga gttgaacggc aggctcacga
    56401 cctaatcaga aaaacgatta aaattcattc acaaagaaat cgaataaacg ctgttcagat
    56461 gaatctacac gatgagaaag ttccggatgc cattggacac caaggatttt gtgcatatta
    56521 tctttacttt ggactgcttc aatgagtcca tcgttggaaa aggcgatggc ttttaacaaa
    56581 ggagaaagat ctttgactgc ctgatgatga taggaattca caaagtatga atcagggaga
    56641 aggtcactta ataaactgtc cgaaacgatt ttgacttcgt gagttgcaaa ctgcggggca
    56701 gtgggctgtt gttcgtgttt tacggaccat tttggatata atgaaagatc ttgataaagt
    56761 gttccctcca acacgacatt caatagttga agtcctcggc agactgcaaa aatcggtttt
    56821 ctttgtttca atgcttccaa gatcaatgcc tgttcgaatt ggtctcgttg aatatttgtt
    56881 tccgtcagtt ttggatgagg gtcttccatg taaagttgcg gactaacatc ctgaccgcct
    56941 gcaagaatga gtttgtcgat tttctcaatt agttgaggcg ctgaatctgg agacatgact
    57001 ggcagaataa gaggtaatcc ttgagcatct aaaactgcat ccacaaatcc ctgcggtgta
    57061 tagcttactt gatttccttg gaaagtatct gtcgctcgaa tcagttgatt cccggcaatg
    57121 ccgatgattg gtttcatttt tattccccct aaagtagact aattgtaaca aggatttaaa
    57181 aaaatttaaa ctatgtaaga cttgccccta tacttatctg ttcttgctgt gttactatct
    57241 gtaaagagaa ttttcgtgtg aaattcatga cgatagttga aaatattgct taatttgtta
    57301 taataaaaag taagcagaaa taaaaaggtt gggcgtaagc ttcaaggatc tagttcttca
    57361 aggggtgaga aggacatttt attgaagggg cctgcctata ctaagttatc tttagaggta
    57421 catactttca atgaaaaaag aaagcatgta cctctttttt aaatcgaaag gaagtgaagc
    57481 aagtgttaga agctttggaa caaatccgaa aagcagaaga agaaaatgag aagcgtgctc
    57541 gaaccctacg gcagcagttg aagacctatg cagaagaaaa agagcaagaa ttggagataa
    57601 tccaaacgaa cagtcggcaa cagctgaaac agttggtaga agaaaaagaa caattcgaaa
    57661 agcaagaatt agaaaaagaa aaaatgaaat tgaaagcaga agcagacaag gtgcaagcaa
    57721 ctttgaaaga ccaatatcaa aagcatcaga gtcaagcaat cggtgcaatc attgaaaggg
    57781 tgaaagaaac ttatggccgt cactaagatg gaaaaagtga ccctcatttc agataaaaga
    57841 aatcaggaga ggattttaca agcagtacaa ggaatccaaa atgttgaaat ccgggatctg
    57901 tatcaaaata gaacaaacaa ccagtgggta gaaaaatatt ttccagatac cacgattatt
    57961 gataaagaag caaaattaag cgctttaggc aatcggctga aagacatcca agaagctatc
    58021 caattcatac aacaccacgg cgatagccgt caaaagaaac tccatttgaa acgtcaagaa
    58081 ctttccttac aagacttgga agcaaattat tccgaagaaa atttcatgaa taaattagaa
    58141 gaagtccttg atttaaaaga aaagtgggaa cagctaactg aaaaacgtga tcgtttagaa
    58201 gaagaggaag aatggcttct tcattggcaa cagctggata ttgctccaag tatgtatgca
    58261 agtaagacga ccatttttga gatggggact gtgagtgcag caaattttga aagtttcaag
    58321 gaagaactaa gtaagcttga tgacgtttat atggaagaaa tagattatac gccaaaacaa
    58381 gtttatcttt cgtatgttgc tttaaaaaaa tcagttgctg ctatagaaga aattgctggg
    58441 aaatacgggt tcaataaaga aacgtatcct tatgaacaaa atcctaaaga aaaattgcaa
    58501 gaaaccaaac agcaattatc cattgttcac gagcagcaaa aaaagataac gacagcgtta
    58561 ggtcagtgca gcggctatat ccaagaattt gaatgggtag aagaagtcac acttgccgtt
    58621 gcagaacggg aaaaagtaaa agaacggttt atccatgcca cttatctgat cgttttgcaa
    58681 ggttgggtag atgcagaaga aaaacagaat ttgatgcatg tgttaaacga aactgtatca
    58741 gaagaagata tatacgttag tttcgaaaca ccaaactcag aagagattca gcaggaagtc
    58801 cctactaaat tgaaaaacca tccgcttatc gaaccttttg aattgctgac tgaaatgtac
    58861 agtctgccaa aatatgaaga agtagatcca acgccttgga tgacaccatt ttatcttgtc
    58921 ttcttcggga tgatggtcgc agatgttggt tacggattgc tgatgctcat tgggacaata
    58981 ctagctcaaa aactatttgt tttaccccgc gggatgaaac gtttcgtaaa attctttgag
    59041 attctctcgg taccatcgat catctgggga ttgatctaca gttcattttt cggacaggca
    59101 ttaccaaaag aactatttgg tatccgcttg cctttcccaa tcctatctac gacggatgac
    59161 gtcaatacga ttttgatttt atccgtgatt ttcggtctga tacagatctt ggttggatta
    59221 ttcgtggcag caaaggaaaa tatcaggagg aaagattact tgagtgctgt cagtgatggt
    59281 tttgcctggc aagggatcct gattgggatc gttattgcat tgattggtgt catgttgata
    59341 aaaaatacag catttgttta tcttggcgcg gggctatcga tcattgctgc tttatgtatc
    59401 ttaatcgtgc cgattatcca atcgccgtct aaagtcaagg gtgctgccaa aggagcgtat
    59461 aatctttacg gattgactgg ttatatcggt gatttagtca gttatacacg tttgatggca
    59521 ctaggtattt caggcggaag tattggcgcg gcattcaata tgctagttgc tttcatgccg
    59581 ccagccgctc gattcagtgt agggatcctt ttgatcatcg ctttacacgc actgaatatg
    59641 tttttgacac tgttaagtgc ctatgtccat ggtgcacgat tgcagtatgt cgaatttttt
    59701 ggaaaatttt atacaggagg cggacgtgcc tttgacccgc tgaaaacggc ggaaaaatac
    59761 gtcaacatca atcataagaa aaaaaacaat aaataattgg aggattttta tacaatgatg
    59821 gattacttaa ttagtcaaaa tggtggaatg gtatttgcag tattagcgat ggcaacagcg
    59881 acgatttttt cagggattgg ttcagctaaa ggtgttggaa tgacaggtga ggcagcagca
    59941 gcgttaacaa caagtcaacc cgaaaaattc ggacaggctt tgattctgca attgcttcca
    60001 ggtacgcaag ggttgtatgg attcgttatc gcctttttga tttttatcaa cttaggaaat
    60061 gatatgtcag ttgtacaagg attgaacttt ctaggcgctt ctctgccgat tgcttttaca
    60121 ggtttgtttt caggtatcgc ccaagggaaa gttgcagcag caggtatcca aattttagcg
    60181 aaaaaacctg aacatgctac aaaaggaatc atttttgctg cgatggttga aacatatgcg
    60241 attttaggtt tcgttatttc cttcctatta gtattgaatg cctaagcata aaggaggaaa
    60301 agcagatgaa cgccattgaa aaaatcatct ctcagatgaa tgaagcagca gagcaagaaa
    60361 gagcggcgtt agagcaagaa gagcgaatga agatcgatca aaactttgaa caaaaacgta
    60421 cgcaaatcga aacagaacat caaaaacaaa aagaaaaaca aatcgaatta cttgaaaaaa
    60481 aataccgtca gcttaggaat cgccaacaag tagaagtacg acaagaaaat ttgaatgcga
    60541 agcaggaatt tttacgacga ttgtttgcag atgcaatagc tgaaatggag aattgggatg
    60601 aatctgaaca aatccagttt atcaaaaatt cattgtattc attacctttg acaggcaaag
    60661 taacattcat tgctggagaa aaatcagcag cttatctttc tcagacattg ctggatgaat
    60721 ggaacaatga acttcccttt atgatggttt taagtgatga aactgttgct gatcaagcgg
    60781 gttttctgat aaacgatcaa ggagttcagt ataattttct tttcagcagc cttgtacaag
    60841 atatccaagg aacgatgagt tttgagattg ccaatcaatt attcgaataa ggagggatca
    60901 gtgtatggaa taccacgaac taaatccctt gattcgcgga agagagcttg aacttttgtc
    60961 caatgagaca tttgaacgga tgattcaaac aaattctgtc gaagcacttg gtgaaatatt
    61021 gaaatccacg atttacagtc catatattta tgacggatat gaatatgact tcgaacagaa
    61081 tcttttaaag gagcagagcc gtctatttcg ctggttaaag gaatcagcac cggagcctga
    61141 aatcgtttgg atctatacga tgcgctatac tttccataat ctgaaggttt tgacaaaagc
    61201 cgagatgaca aatcaaaatc tggaccattt gtatatcgac gatggatttt attcattaga
    61261 aaccttgaag gatgccattc atacgcaagt ttcaaatgaa ctccctgaaa gcattatgga
    61321 cagtatacga gaagtacgtg attattttga agagtctacc attcttcagg gaattgacgt
    61381 gatttatgat cgacagtttt tgacagagca gcgacggctt gccgataagc taggatatca
    61441 agaattgctg acagaagtca ttgcttttat tgacttgacc aatatcacaa cgatggctcg
    61501 aggaatattg cagaaaagaa catatgggtt catgacgacg gtactttcca gctcgggaag
    61561 tatcgaaaaa gaagtatttt tgaactttgt agataaagat atggaagatt acacgaattt
    61621 tctattagag acagattatg ctgattttct gcgccctgtc atccgtgagg gaaatcttga
    61681 tctcgtaaag cttgaacagt tgaaagatga ttatctaagt tcattgtatc aagaagcaca
    61741 aacccaggct ttcggaccac tgccactact agcctttttg aacgcaaaag aggtagaaag
    61801 caaaaacctt cgtttactgg ttatcggcaa aagaagccat ttcgaaccag aaacaatcag
    61861 aaagcgggtg agacaagtct atgacgcata agatcggtgt agttggcgac aaagattctg
    61921 ttttgccatt caaattgttt ggctttgatg ttcgttatgg tacaaccaag caaaccgttc
    61981 gtcaggcaat tgaagaaatg gcaaagaatg agtatggcgt gatttatgtc acagagcaat
    62041 gtgcagcatt gagcccagaa acgatcgaac gttacaagga acaaatgata cctgctatcg
    62101 tattgattcc caatcatcaa ggatcattag ggatagggct ggaagaaatt caaaaaaacg
    62161 tagaaaaagc agtaggtcaa aatatcttat agtatgaaaa ggagatagaa attttgcaaa
    62221 ttggaaaaat cataaaagtc tccggacctc ttgtaatggc tgagaacatg tctgatgcca
    62281 gtatccaaga catgtgtcta gtgggagatc taggagttat cggagaaatt atcgagatgc
    62341 gtggcgatgt ggcgtctatt caagtatatg aagagacttc tggaattggt ccaggcgaac
    62401 cggttcgctc tactggtgaa gctttatctg ttgaattagg accagggatc atttcacaaa
    62461 tgtttgacgg aatccaacgg ccattagata cctttatgga aatcacacaa agtaatttct
    62521 tagggcgcgg agttcaattg cccgcattgg accatgagaa aaaatggtgg ttcgaaccaa
    62581 cagtagaagc cggagaaacg gtgagtgctg gagatgttat cgggatcgta gaagaaacaa
    62641 aagttataaa acataaaatc atggtgccaa atgggataaa aggaacgatc aaaacaatcg
    62701 aaaaaggttc attcactatt gatgaaacag tatgtgtcat tgaaaccgaa aatggtgaca
    62761 aaaatttgac gatgcttcaa aaatggccag taagacgtag tcggccaatc aaagaaaaat
    62821 taaacccgga tgcacccatg atcactggac agcgagtgat tgatacattc ttcccagtga
    62881 caaaaggagg agcagcagct gtacctggtc cttttggtgc agggaaaaca gtcgttcagc
    62941 atcaaattgc aaaatgggcc gatgtggaca tggttgtcta tgtgggatgt ggcgagcgtg
    63001 gaaatgagat gacggatgtt ttaaatgaat tccctgaatt gattgaccct aatacaggag
    63061 aatcgttgat ggaacgtacg gtattgattg ctaatacatc aaacatgcca gtagcagctc
    63121 gagaagcttc tatctatacg ggaatcacaa ttgcggaata tttccgtgac atgggttatg
    63181 acgttgctat catggcagac tccacttcac gatgggcaga agcacttcgt gagatgagcg
    63241 gacgtttgga agaaatgcct ggtgatgaag gttatccagc ttatcttggt tcacggcttg
    63301 cggaatacta tgaacgatct ggaagagtca ttgcattagg atcagaaggt cgcgaaggaa
    63361 gtataaccgc aatcagtgct gtatccccgt ctggagggga tatttcggaa ccagtcacac
    63421 aaaatacatt gcgggtcgta aaagtattct ggggtcttga ttccagttta gcccaaaaaa
    63481 gacattttcc ttcgatcgat tggatacaaa gctattctct ttattcaaca gaagtaggga
    63541 agtatatgga tcatatcctt caagctgact gggcatccat ggtcaccgag ggcatgcgta
    63601 ttttgcagga agaacagcag ctgaatgaaa tcgtgcgttt ggtagggatt gattcccttt
    63661 cagataatga ccgtctgaca ttagaagtgg caaaatccat tcgtgaagat tatttgcagc
    63721 aaaatgcttt tgatgatgtc gatacattca cttcaagaga gaaacaattc aaaatgttga
    63781 agatgatctt gacctttggt gaagaaggac gccgtgcgtt gtcgttaggt gcttatttaa
    63841 gtgagatcat ggacggtacc gtatcgattc gtgaaaggat cggacgaagc aaatatatcc
    63901 cggaaacaga attagaaaaa ctagatcaaa ttattggtga aatcaagcaa acgatgcaaa
    63961 cgatcgtttc agaaggaggc atgagcaatg attaaagaat atcgtacgat cggtgaagtc
    64021 gtcggtccat tgatggcggt tgaaaaagtt gctggtgtca aatatgaaga attaatcgaa
    64081 gtccgtatgc aaaatggcga gattcgtcgt ggacaagtat tggaagtcca agaagataaa
    64141 gcaatggtcc aaatattcga aggaacaagt ggtatcaatt tacgtgactc ttctgtacga
    64201 tttttaggcc atcctttaga actaggtgta tcggaagaca tgatcggtcg agtattcgat
    64261 ggtttgggac gaccgaaaga taacggacca gagatcctgc ctgaaaagca tttggatatt
    64321 aacggagaag taatcaatcc agtagctcgg gattatccag atgagtttat ccaaacaggt
    64381 atctctgcaa tcgatcattt gaatacatta gttcgtgggc aaaaattacc agtattttct
    64441 ggctctggtc ttcctcataa agaattagct gctcagatcg cgcgacaagc aacggtactc
    64501 gattcatcag atgatttcgc cgtagtcttt gctgctattg gaatcacttt tgaagaagct
    64561 gaatttttca tggaagattt tcgtcaaacg ggcgcaatcg accgttctgt catgtttatg
    64621 aatttagcaa acgaccctgc aatcgaacgt atcgctactc ctcgtatggc tttgactgct
    64681 gcagaatatc ttgcttatga aaagggaatg catgttttgg ttatcatgac agacatgacc
    64741 aactatgcag aggcattgcg agagatttct gcagcacggc gagaagttcc aggaagaaga
    64801 ggatatcctg ggtatcttta cacgaactta gctactttat tcgaacgagc aggaagaatc
    64861 cgcggactaa aaggatcggt tactcaaatt ccgattctaa caatgccaga agacgataaa
    64921 actcatccaa tccctgactt gactggttat attacagaag gacagatcat cttgactaga
    64981 gagctttaca agagtgggat tcagccacca attgatgtac tcccgtctct ttcgcggtta
    65041 aaagataagg gaactggacc cggaaagaca agagaagacc atgcagctac gatgaatcag
    65101 ctttttgcag cctatgcaca aggaaaacag gcaaaagaat tagccgtagt tttaggtgaa
    65161 tctgctttat ctgatgtaga caagatctat gcgaagtttg ctgagcggtt tgaaaaagaa
    65221 tatgtcaatc aaggattcta cacgaaccgt tcgattactg aaacacttga tctagggtgg
    65281 gaactgctat cgatgttgcc aagaaccgaa ttaaaacgaa tcaaagacga catgctggac
    65341 aaatatctgc cagaagggaa gtgagttctg atggcacgat tgaatgtgaa tccgacccgc
    65401 atggaattga ctcgattgaa aaaacagttg acaactgcca caagagggca taaattatta
    65461 aaagataagc aagatgaact gatgcgtcaa ttcatcctgt tgattcgaaa gaacaaccag
    65521 ctgcgtcaag aaatggaaga gtctatgaca gaagcaatgt ctgattttgt attggcaaat
    65581 gcttcgatca cggaaccttt catcgaagaa ttatttgttt tgccggcgga aaacgttgat
    65641 ttgtctatcg tagacaaaaa tatcatgagt gtaaaagtac ctgtgatgaa ttttgaatat
    65701 gatgaacagc tgacagacac acctttggaa tacgggtatt tgaattccag tgccgaactg
    65761 gatcggtcaa tcgatcggtt tactaccctg cttccaaaac ttttggaact gacagaaatc
    65821 gaaaaaactt gtcaactgat ggcgagtgaa atcgaaaaga cacgtcggcg agtcaatgca
    65881 ttagaatata tgacgatccc tcaattagaa gaaacgatct attatattcg tatgaagctg
    65941 gaagaaaacg agcgcgcaga agtaacaagg ttgatcaaag taaaaaatat gggtaccact
    66001 gactaagtgg tatctttttt taaaagagga aaacgatgat taaacaaagc aaacaaataa
    66061 agaaacgttt gccggctgtt cagcttctcg cattaggatt ttttctcctg attttagttg
    66121 gcggcggcct acttacccta ccatttttta gtaatagtgg gcatgggaca aattttatcg
    66181 atgcgctttt cactgcaact tctgctgtct gtgtaacggg attgacaaca ttgaatacag
    66241 cagagcattg gaacagtgct ggtcaatttt tgatcatgct tttgattgag atcggcggtc
    66301 ttggttttat gatggttccg attctatgct ttgcattgat aaagaagaaa gtcagtctca
    66361 gcacacgtat tatattaaaa gaagcgctga atctagaaga agtatcaggt gtgatgaagc
    66421 tcatgcttta catcctaaaa ttggcctttg ttatccaatt gattggtacg gttgctttat
    66481 cttttgtatt tgtgcctgaa tttggctggt tgaaaggtat ctggtacagt ttgttccatg
    66541 caatctctag tttttgtaat gcagggtttg atttgttagg agacagttta gccaatcacc
    66601 agcaaaatgt ttatttgatc atggtggtat ctgctttgat catttctggt gggttaggct
    66661 tcatcgtatg gcgtgattta ctggaatatc atcagaaaaa gaagatgacg attcattcaa
    66721 aaatcgcatt aaccgttaca ggtacccttt tattaggcgg attcgttgtg ttctttttca
    66781 cagagcgcaa tggggcaacg ctagtggaag ggaatatgtt tcaacgattg gcaaacacct
    66841 tttttatgag tgtgacacct agaacggctg gatattattc agttgattac ctccatatga
    66901 gccatgcagg tctgatttta acgatgtttt tgatgtacat cggcggaaca tctgggtcga
    66961 cggccggcgg attgaaaaca actacgttag gtgtgttgct gatccagatg cgttcgatgt
    67021 tgaaaggacg tacgagagcg gaagcatttg gccgtaccat ccgacaaggc gcagtgttgc
    67081 gggcacttac tttatttttt gttacgttga cgttgtgtgt agtcgccatc atgattttat
    67141 cggtgactga aacgatacct aaaacttctg gcatcgaata tattgctttt gaggtcttct
    67201 ctgcgtttgg tacagtaggt cttacgatgg gattgacgcc agacttgact gcaataggga
    67261 aattgatcat tatagcgctt atgtacattg gacgagttgg tgtattgact gtggtcttct
    67321 ctctgatgac aaaagcacaa agaacacaat caaaatacaa atatccagaa gaaagtgtca
    67381 tgattggata atttcatctt ctgacagaaa cctagtgatt tagtaaaata agaaaataaa
    67441 ataatttttc ttgcttcttg gatactttaa gtgcaaaatg ttcgtttatt ttttgaggca
    67501 ctacccgttg ttgaacctcg ggtagtgcct cttttttatt gaaaggaatc aataagattt
    67561 gattccttta tttatcaata ggttataatt catatttttt tattttttct tttacgatac
    67621 ttagtccaga agctaaaatt tcaaggttat tagaggatag ggaaattcga acgaatgcat
    67681 ctggttgcgc acgtgtagta aagcgtctgg aatgataaag tcggataccg ttagcaagaa
    67741 agtcattctc aattatctta gggtcaatct caggatcaac tggaagaatt cgatagtaag
    67801 ggacacgatt cgttggtttt tctactttga aaagagaatc aaaaagctga ttggctcttt
    67861 ttgtaagact taatttagtt tctaagatcc tatttgcttc atttgaatgg aggatttgag
    67921 tgatgacttc cacatctaat ccagaagttt tcacatttat attaaaaatt gcttgttcta
    67981 tcttgctttg gacatgtact ggaactacaa gataagcaac tcgcaatcca gaacaaataa
    68041 attttgtcat tccagctaag tagattgttt ggttaggtaa taaattttgg aaaggaggaa
    68101 tgatcgtatg ttgatagtaa gttgttaaaa atgaatggat atcatcttca ataacccata
    68161 gaccttcttt ttcaatcact tcggctaatt gctctcgtct tttgtgagag atttgaaacc
    68221 ctataggatt attacaagaa ggcataagaa agacaccatg aatcttcttt tttctacatt
    68281 cctgaatcaa tatatcaggt cgcattcctt gctcgtcaaa atcaattgga acgatctcta
    68341 aatgatataa ctgagccaat tcaatgaaat tagcgtatgt atatcggtct gttgctattc
    68401 tatcacctgg agagaaaagt gcagaaagtg tgatagccaa tccattttga acaccggaaa
    68461 caatagatag agattctaag gacggaatga ctccttgagt ttttaaccaa tttgctgcag
    68521 tagtcaattg aaaatttgtg cctttgggat cttggtaatt aagcaaacca acaagatttg
    68581 ggttattact agttgattga ataaacggga caacgaattt attacattct tcaaaagaac
    68641 taaccagacc aaaatcaatg atatgttcgg ttacttcatt ggtagagatg gtgatggact
    68701 caaagacatt gggagaaata aatgtgccac ttccaatatt cgtatacagc aaacctttct
    68761 caattccata tttataagcc ttgccaacgg tagtaaaatt taaatctaaa taatcagcta
    68821 gctctctttg ggagggtaac tgagtgtttt tctgtaattt attctttaaa atatcttgtt
    68881 ctaattgttg aatcaacgat cgataataag gacgcttcaa tttttgtttg tctggtcgcc
    68941 aagttaacgg gtaattgtca taagaattaa taggcatttt ctccactcct taaaattgta
    69001 taccatacaa ttttaccatt gattgtattg attttagaag ttaatctttt gtgaggaggg
    69061 aattgtctat gaaaattatt aattttgagg aagtagtagc ttcttttagt tttgaagaag
    69121 cgattcaagt aatgaaaaaa tgtttttttg attatgaaag tggtaaaatc agccaaaatc
    69181 ctcgattggt ggaaatttta cctgacggaa aaggcaaaaa tatgtttgcg atgatgcctg
    69241 cttatttggg ggagaacaga tattttggca caaaagtcat taccgctttt ccggataatc
    69301 atcagtatca tttaccttct catttaggag agattttctt gtttgatgct caaaacggac
    69361 tacccaaagc attgataaac gcaaatgcag tcacctggat tcgaacagct gctgtatctg
    69421 cgctggcaac agattactta gcaaatccgc atgcagaaac acttgcttta attggagctg
    69481 gtcaacaagc attgtcgcat ttgactgcga tttctaccat tcgtcccatc actacagtgt
    69541 ttgtttacga tattttagaa gaaagacgaa atacttttat cgctaaagct aaaaaacagt
    69601 atcctaaaat aacgtttata aatgcaatat cagtagaaga tgcagtacgt ccagcggaaa
    69661 tcatttgtac gttgacctct agtaaagagg catttcttca aaaagagtgg gtaagggaag
    69721 acgcacatat caatgctgtg ggcacattta ctcctgatac aagagagata agtacatcgt
    69781 tgatggaaac ttctcaaatc tatgtagatg attactcagc tgccttaaca gaaagcgggg
    69841 acctgcttat agccatggca gaaggagatt tagaggaatc ttgtattgtt ggtacattag
    69901 gcgaattagt ttctgagaaa aaaacatttt ataaaggaga atcgaggccg acgattttcg
    69961 acgcagttgg tctagctatt gaagatcttt gttgtgcaga atatatttat aacaaattag
    70021 agaaaggggc tttaaaatga acttagatta ttacgtagtt gatgcatttg cagatgaggt
    70081 gttcaaagga aatccagcag cggtttatgt gctagaagaa tggctgccag aaggtacgat
    70141 gcaaaaaatt gccattgaaa acaatttatc tgaaacagca tttacagtga agaaaaacca
    70201 agaatttgag cttagatggt ttacgccaga tcgagaaatc gatctttgtg gtcacgcgac
    70261 attagcaaca gcatttgttt tatttaacta ttacaaaata cctgatgaaa cgattaaatt
    70321 ttctactcaa agcggtaatc tatttgtgac aagaattaaa gattattatt acatggattt
    70381 tcctagtatt atgccgaaaa aagtgccgat attgtctgaa tatgaagaag cgattggggc
    70441 taagattaaa gaagcctact tagcacgtga tttatttttc gtattagaag atgaaaaaac
    70501 ggtggctaaa ttgcagccag attttacagc tatcaaaaat tttgaattag gaataggagt
    70561 catcgtgaca gcagaaagtg tgcaaaaaga ctttgtttct agaaccttct ttccaaaatt
    70621 aacaatcaat gaagatccag tatgtggatc agcacattct aatcttattc cttattgggc
    70681 agaaagaatg aataaaacaa aattgtcagc ttatcaaatg tcatcaaggg gcggggatct
    70741 agaatgtgaa ttaaaagaag atcgtgtgat aataggagga aaagcagttt tgttttctaa
    70801 aggtacctcg tttatttgat ttctttcaaa tgaattaaaa ataaattggt ggagagtggc
    70861 aaccttatag ctacaatggt tgttaaaaaa agacgcaaac tttttcaatt taatgaaaag
    70921 tttgcgtctc attttaatta ttggtgtttg ttttttattc aatcaagagt aaaatgcccc
    70981 atactaacta tcctcggatg acttcgattt tataaccatc agggtcaatg acaaagtagt
    71041 aagaaggcgc agtgccaggt aatcctttta agtcagtaat ctcaagaccg gcagctttat
    71101 gttgctcgtg taatttttct agatcatcag ctgctatagc gatatgacca tagccgtctc
    71161 ctagatcata cgcaccatga tcatagttgt aagtcaactc taattcatag tcatccccag
    71221 gaagtgtcaa ataaacaagt gtaaatttgt tttctggaaa atcacggcgg cggctttctt
    71281 caaaaccaaa tgccttttga taaaattcga ttgatgcttc aagatctttc actcgaacac
    71341 atgtgtgagc cattttcata gattcatctc tccttttcct tttactcaat cataacaaaa
    71401 agaagtaagg aaatagttga tttgtgcttg ttcatgagaa atttctcatg taaaatggat
    71461 agaaaataaa actgttaagg tggaaactat tcatggataa accaattttt ggtgaaaaaa
    71521 agcaatttgc tgattatcaa acccgttatg cagcatatat tgtcatagaa agagatcagc
    71581 agattgcagt aatcgaggct ccaaatggtg ctttttttct tcctggcggg gaaattgaag
    71641 gagaagagac gaaagaagaa gcgattgtcc gtgagctgat agaagaaatg ggaattgtgg
    71701 cagaaatttc ttcttatctg ggagaagctg atgagtattt ttactcgaat tatcgtgcaa
    71761 cttattatta taatccagga tatttctttg ttgctgattc ttggaagcgt ataggagaac
    71821 caactgaaaa gaccaataaa atttggtggg tgagccctca agaagcgctt gtgaaattga
    71881 aacgtggtag ccaccgatgg gcagtcgaga gatggatcga aaaaaataaa aactgaaaga
    71941 aagcgagtag atcagctttc tctcagtttt tttatttatt acagtacata ttttttgatt
    72001 gcttcagcta caccgtgctc gtcgtttgat gaagtgatga catttgctgc ttctttgaca
    72061 agagaaacgg catttcccat tgctactcct agaccagcat attcaatcat ggataagtcg
    72121 ttctcgttat ctccgatagc catgatttca tcttgatcaa taccaaggtg ctgtgcgaga
    72181 ttcgcaactg ccgcaccttt acttgcttct ttatttagta cttcgaagta atagggcgca
    72241 cttttgaccg ttgtgtattt atcacggaaa gtttgcggta aacgcgcaat tgctgcatct
    72301 aaaatttctg gttcgtcgat catcatcatt ttgacgatgt tcatgtctgg tgtcatttct
    72361 tcaggtgttc gatattttag cggcatcttg actaaacttg cttcgtggat cgtataagcg
    72421 ctgatatctc gattaggcgt gtagatcgct ttgtcatcga tcgaatggag atgactgccg
    72481 acacggcgtg ccatcacttc gatctctagg aaatcttcat gtgtcagtcc gtgacgggcg
    72541 atgattttac ctgatttcgt ttgttgtacg agtgccccgt tataagtaat aacgtaatca
    72601 ttatcttgaa aaaggttcaa ctcgtttaac tgttcctgga cacctggtaa gggacggcca
    72661 gtgcataaaa cgatcgaaac accttttgca acagcagcgg caatcgcttc ttttacttct
    72721 tttgttactt ctcttttctc atttaataaa gtaccatcga tgtcgatagc gactagtttg
    72781 atagacataa tttcctccaa tatattttaa tttatttcaa tcaatgcgcc attacggata
    72841 tggctggcaa attcttgata agtttcgtca aataaatcat aatgatcacg caaggacgga
    72901 tcaaccattt cttttggaaa gaaaaatcgc tcatctcctt gttcttgtcc cgctaatgca
    72961 gcaacaagct gactaacctg tgataattct atcaaagttc cgtcatttcg caaaatctcg
    73021 atttgtgtgc gatgtctgcc ttgttctggg cgataaaaat cataaggtag atcgtagcta
    73081 gaattcacag cagtgtaata aacaggatta tatccgacct tttctactaa caaagtgagt
    73141 tcttggacta attttgaatc ccggttgcca gaaaaagtag cagatttcag tggtttacga
    73201 ttcaaaaaac gtttagctaa atcatttaaa ataggatcag cagattcaga ccattgagta
    73261 aagtaagtgc ccaagacccc atcatcaagt ttgaggtatt cttctaaagt aaagcttccg
    73321 tcaagaaatg gcagaagtaa ttgagaatgg agttcaaaac tgctggcatc ttcttgatac
    73381 aactcgtgtg cgcgatgaag cagatgctcc aaaattactt ccataccacg ggaaacagga
    73441 tggaagtaca cttgcacata catttgatat cggctaacga tataatcttc cactgcatgc
    73501 atgccactca tggaaaaagc gatcccgcct tcataaggac gaatcacacg taatattcgg
    73561 gtaagatcaa atgttccata ttctgttcca gtgaaataag catcccgaag caaatagtcc
    73621 attcgatctg catcgatctg actagaaatc atttggacaa cttgtgggtt ggggtaagtt
    73681 ttttggatca cactagctac cttttcagga aaaccttctt ctactcggtt aagaatctga
    73741 tacacttctg tttctggaga agtgatgatc tctacagtga tggcttcatg attcgtatgg
    73801 aagatatgtt caaatgtatg agagtaagga ccatgaccga catcatgcaa cagtgccgca
    73861 cataaagtca ccagccgttc ctgatcatcc cagcctccgt tgcctatttt ttcttttgaa
    73921 aaatttcgag aaaagatatc gcagattcgt cttgaaatct cataaacgcc taatgaatga
    73981 gtaaaacggc tatgttctgc tccgtgaaaa gtgaaagaag aagtgcctaa ttgtttaatg
    74041 cgtcgtagac gttggacttc tttcgaattg atcaaatcta agattacttg atgctggaca
    74101 tggatatagt tatggactgg atcacgaaaa actttttcca taggcagcat ttgatattta
    74161 taaggggttg tcataatgta gctcctttat gttattacgt tgttccattc tctggatttg
    74221 gttgttccat tgctccagct ggatttcttt ttttagccag tcggtagaat cctgtaaatc
    74281 acttgcagaa tacagctttt caaaagcttt tgcaaatgct tctttgactt cttttacaaa
    74341 aaggggttgg tcaagcagtt cttcaagtgt tgccattgta gcaggatcca ctgcaggata
    74401 gccatttgta ccaaagtctt cttttaaagc agattgataa aatcgcttca tgacttgccc
    74461 gcgtttttcc tgattaccgt tgacacttaa gtacatcata acagaaatcc catttttgat
    74521 ccgacgctga gcgataccgg caaatttttt gccatggatg cttaaatcat aagttccagg
    74581 acaataagaa tgggtgattt cgaatgcttg tatcggtgcc tttggaaaag cgtatttagt
    74641 caataggagc atttgctcgt aagcgtcatc tatggttatt ttttttgtgt cactttgggg
    74701 gaaaatcaaa gaaatattca aaattccttc atctgatacg atccctaatc cacctgaatt
    74761 tctgatcact ggagcgtaac ctgcatcttt cagtgtttgg atcccgtcac ttagaaaagg
    74821 agttcgaaca tccttcatac ctaagatgac tgtgcagtca tattgccaga aatgtaaaaa
    74881 gatttgctgg tgttctttag aatattccgt taatatatct gttaatgcaa aaggggaaaa
    74941 gtcgtttttt tgataaatgt gctgatctag tcgaatcgca gggttcattc aaccggctcc
    75001 tttgtataaa ttcatatttt ctacaatatt atactctaaa atcaaagagt tgttttggaa
    75061 aaaaggtttt ctgtaaaaat ggacaagttg gttgacaaaa caaaagaaac taggaacaat
    75121 gtagctatga gaagtaaagg agaatgaata atggaattat cacaaggaac accgatttca
    75181 atcaaattgc gtacaaaagt aaaacagcaa ggggaagtgc aagattttta ttttgatttg
    75241 aaagggcaaa tggtgacaat tggcgatacg ctgtacatac gttataaaga gattcaggag
    75301 gagacgggcg atgagattcc tgtaacgatc aaattagttc cagatggtca tgtacagctg
    75361 atccgtgcag gagaaatgcg gatgcgtttg aaatttggct ataaagaacg gttagaaaca
    75421 tcctatcgaa caccatacgg tatgatccag attgctactt ttacgaaaga actgcatgtt
    75481 agtttgaaag accgtcccac aggagggaag gttcgaatcg attatgatct gttcatggga
    75541 ccggaaaaaa tcggcgaata ttatttgacc ttggatttta cagcgtaaat ataaaagaaa
    75601 agtttgattt tttgtgtctg attttgtatg ataaggagta gactgtgaaa ggacgtgtcc
    75661 tgattggaaa ttagtgtatt tgaaggtgca aacaaaagcg aattatcaat gatcgaagta
    75721 gcacacgcga tcttagaaca acgtggcgat gtcatggatt tttctgattt agctaaccag
    75781 atccaaaact atcttgggaa atcagacagt gaaattcgtg attcactcgc acaattttat
    75841 actgacttga atattgacgg cagctttatc tcattagggg ataatcgttg gggcttgcgt
    75901 tcatggtatg ctatcgattc gatcgatgaa gaagtaaacc atggaatgga cgaagaggat
    75961 gaagatactc cacgtcgccg taaacgcaag aaagtcaacg cctttatcaa tgatgatgaa
    76021 gacgcgatcg attacaatga cgatgaccca gaagatacag atctgacaga agaagatgac
    76081 gatgatctgt ttgatgacga tgatgatgaa gatgaagaaa tcgctgctta taattctgac
    76141 ttgcaagaaa tcggagcaga tgacccgaca gatgatgaag aagacttgcc aggaaatatc
    76201 gaagaagatt taagtatcat agacgaagac gatgatgatg aagattatga agaggaagaa
    76261 ttttctgact ctgaaaaaga ataaacatta ttttcttacg agaggttgtg acagagacga
    76321 tcagcctatc agaataagga ggtgtgacac gatttttgtc acaccttctt ttttgtatat
    76381 aaaaatccca agtaaataca taaaaaataa aaaaattgaa acttttagcg caccttgtac
    76441 gtcatattaa gtgaggcaga aaggagtgga gaatgatttt tactggatat aagaaaaaac
    76501 ggcagatcga aaaataccgt cacctgctga atggactcat cgaaactgat tatcaaaaaa
    76561 tgttccgtat cacgatgaat tacgtacatg ataaagaaga ggccttagat gtgatgcaag
    76621 atagttttca taaagccctg aactattttg aaaaagaaaa agagatcgag cattttagtg
    76681 catggtttta tcggatattg atccgcacgg cattagatag ttggcgcaga caaaaaagga
    76741 cgcctgtgga cttatttgaa attcaagata tcccccaaca gttaccttta gaaacttccg
    76801 ttgcagagct ccatatgatt ttagataaaa tagacagtcc ggaaaaagag attctgatcc
    76861 tctacttttt tgaaggattt cgtttgaaag aaattgcgac tatcttggat atgaatgaaa
    76921 acacagttaa aacgaaaatg taccgctcat tgaatgcttt acgtcaaatc ctagaatgaa
    76981 ggagagaaga gtgccatgta ttcccaaaaa gaaaatttaa aaaaactaaa gaaaacttat
    77041 aaagaacaag caattcccaa agggcttaaa gaggagatcc gccagcgttt tattatcgaa
    77101 gagcaatcat ttataaaagc aaagcggaga aagagacgtc tccaagggtt cggagtatct
    77161 ttggctatcg taatgattac aacaagtagc cttcttttta atagtcaagt tcgaagcttt
    77221 gcagaagatt tacccatctt aggatcagtc attgaattga tcttaggcga acgctttact
    77281 gatcgatctg aaaaaatcga tattcaggta ccgaagatca atacacaaaa tgagaaagaa
    77341 aataaaacaa ttcatggatt aaatcaaaaa tactttcgcg aaggacaagc agattttgaa
    77401 aaagctgaaa aagaatatgg aaattttgaa acggatcatt accaagtttt gggagattat
    77461 cagaaaatcg tagacgataa ccggtttctc gtgattgaaa ggcagatcac ccaaacagca
    77521 gcagatagcc atgttgaaaa aagatacgac acaatcgaca agaaaaacag tgtccagctc
    77581 tcacttccac ttttattcaa agatgacacc tatctatctg ttttgacaaa agaagtcaaa
    77641 cggcagatgg ccgaacaagt caaggaagat ccatctaagt actattggac tgagcaagat
    77701 attcaagaag ggacgataga aaaaccaaca cttgttacgc ctacacgaag tttttatttg
    77761 aacaaagagc atcaattagt tttgactttt tctcaatacg aaattgctcc tggttatatg
    77821 ggtacaccag aatttgtcat tcctaagtcc gtgacaaaaa cgattttggc atcggaagat
    77881 tatttggatc attagacgag aagtagttgc gactagcagt ttcaattttc aagtaagtac
    77941 aagtaaataa taagaagaga aaaagagaaa gaagagatat agtgaaaaga aattatttag
    78001 ttatcagtat tattgcttta tccgtgcttt tgtttttcga aattacttcg gtggcctcct
    78061 tgatcaccga agtaaaagga caaacacact cttcggagca ttcgtcacaa tctttcaaac
    78121 aatcagaaca atcaaccatt aaaagccaat caactgaaac agctaacgtc aaagaagcca
    78181 ttcaagaatc agcggaaaag gtacaaagtc agtcatcaca atcaacacaa gacagtacaa
    78241 aaacgagccg agatccggct gtcaaaagcg tcgcaatcag ctttgatgat ggaccaggag
    78301 cgaccacaac accgcagctt ctgcgaatct taaaagaaaa aaacgtgcat gccacttttt
    78361 tcgtcttagg agaaaatacg gcgcagcatc ctgaaatcgt gaagcagaca gctgaagcag
    78421 ggcacgagat tggcaattac acatacgatc atcagaatct ggccactctt tctgcccagt
    78481 cgatgacgga ggaagtgacg aaagcagaca ctgagatcaa aaaagtcact ggcaaaaccc
    78541 ccacttttgt tcgtccgccc tatggttctg tcacgaatgt aggagcaacg atcatccagc
    78601 gaccgatcat cgaatggtct gttgattcag aagattggaa gacaagaaat cctgatttga
    78661 ttttgcagaa gatccaagca acagtatacg atggggcaat tattttattc catgatatct
    78721 atcctgaaac gattcgtgcc gttcctcaag tgatcgatta tctgaaagag caagggtatc
    78781 gcatcacgac agttggggat ttattagggc atccaacggc tgtcgagaac tattatggca
    78841 gaaacgatca tcggccagtt cagtaagtga gacaagtatc tttaataaat aacacatttt
    78901 aataagtagt tgaacacata actaccttac ttgcaaaggc ttaacaatat ctatacaggt
    78961 acaacaaaaa actgtccacc aattttcttt ggcggacagt tttcacttaa aaataaagaa
    79021 gcaactattc gtaaattatg ctaaaatagc agtagaacgc gtcctgaggg aatcgaaccc
    79081 ccgtctcaag aaccggaatc ttacgtgata tccactacac taaggacgca agcactggga
    79141 taattgtaac taaacagata aaaaattaca aggggattgg acaaaaaaat gaaatttgag
    79201 caaaattttt cacaaaaaca gcagcagaca cagaaactag ccatgacgca acagttgcaa
    79261 cagtccatcc aagttttaca atataacagt gaggagctgt tggcattcgt tgagaatcaa
    79321 gcaatggaaa atccattggt ggaagttgtt gagccggaat ggcagccaga ttacataaaa
    79381 gcttcttcat cttcttatga aggagaagag acaaattacc taaatcagat tcctgataca
    79441 aaagggtctt tatttgattc attgatcgaa caagtccatt tgaattaccg agatactttt
    79501 ttgaggaaaa tcgttcttta tcttgtagaa tacatcgatt tgaacggatt tttgacgatt
    79561 agtttagaag aagccggaaa agagattgga gctacgccta tccaaatgct cgatgcgttg
    79621 actctgattc agcaattaga accagctgga gtaggtgcac gtagtttgca agaatgtctc
    79681 atgcttcaaa cagaacgaga tgattatgcg ccggaattag cttatatcgt gttggaagaa
    79741 tgttttgagg aactggtgga gcgaaaatgg aaagaaatcg cacaacgttt ttctgttgat
    79801 ctacatgctg ttcagcagat attcgactat ttacaaacgt tgagtccaag ccctggccgg
    79861 attttcgacc gtagcagcga attgtacatt attcctgatg tccgggtatt agtggacgac
    79921 gataaaaatg tacaagttat ctcaaaccga aaaaatcagc caaatatacg ctttcaagag
    79981 agttacttca aacaaatgtc acaacaagca gataaagaaa ctgaaaatta tttaaaagaa
    80041 cggaaacagg aattcgaatg gttgaagaaa acgatcctac aacgtgggga tacaattttg
    80101 cgggttgctc aagtcattgt ttcccggcaa aaagctttct ttatcgacaa ggaacgtcca
    80161 atcaagcctc ttacgttaaa ggaagttgca actgagatag atgtgcatga atccacggtc
    80221 agtcgtgcag tcaacgggaa atatttggaa acagatttcg gtgtatttga attaaaaaaa
    80281 tttttcacta cacgaattcc tacgaatagt acagaacaga cagaagacct ttcagcagat
    80341 acagcgaaga aaaagctgca ggaattagtc gatcaagaag acaaaaacaa gccgttgtcg
    80401 gaccagaaat tggttgaatt gctgaaaaaa gatgagatag cgatctcaag acgaacagta
    80461 gcaaaatatc gtgatttatt aggaatcccg agttcatcaa aaagaaaacg ttatgataac
    80521 taatcagtca aaagactaga aaaagtattt cactttttct agtcttttta gttgaaaaac
    80581 gagagcgaaa ctgttagact tacagtcgtg aggttgagat ttccctttat tgatgtgaaa
    80641 ttcgaatagt taatttcttt ttttatcgta tatgggtcgt tttaagtcta cattggacgt
    80701 aaagtgtcca actaaggaga tcgctatgct ggatgaaata aaaatgattg aatctgttgc
    80761 accagatatt atcgaagtgt tgcaagaacg ctataaaatt ttacgaaata tctattggat
    80821 gcagccgatt gggcgtcgaa gtttatcgga aagtatgggg atcacagaac gagtcttacg
    80881 aacagaaaca gaccttttga aaaatctgaa tttgatcgac acctcaaaaa gcggtatgac
    80941 gctgacaact aagggcgaag aagtttatca aagccttgaa aacttcatgg atcagctttt
    81001 aggtacgcat caaaccgaac aacagttggc agaatatttt ggtaaccaac gctgtatcgt
    81061 tgtttcagga aacagcgaag agcagaccaa agtggctgat gcattcggcg aagcgctgag
    81121 cgaagcctta gaccgactac ttccagaagg cgagaatatc atcgctgtga tgggaggaac
    81181 tactatggct gtggtagccg aacaattgtc taatctggaa aataagaaac ggcataacct
    81241 gtttgtccct gcaagaggcg ggattggtga agcgataact gtacaggcaa attctgtcag
    81301 tgcaagaatg gctgctaaag caaacggtaa ccaccgcgca ctttatgttc cggaacaatt
    81361 aagtctggca acttacaatt ctttactcaa tgagccatct atccaagaag tcttaaactt
    81421 gattagtgaa gcgaattgtg ttattcatag tattgggcgt gcattacaca tggcagcacg
    81481 tcgtaagatg actgaaaaag aattagtcat gctgaaacaa gcaaatgctg ttgcggaatc
    81541 ttttggctac tttttcaatg aaaaaggtga agtcgtttat aaggtcccta gaatcggcat
    81601 agaattgcaa gatttggaaa aagtcccgat catcatggca atcgcgggcg gaaaatcaaa
    81661 agctaaagca attcgagctt atatgaaaaa tgcaccaaaa caaacgtggc tcatcactga
    81721 tgaagctgct gcaaatgaga ttttaaaagg ggtaaccctt taaaataaat atttttttga
    81781 ttttcataag gaggaaatct taaatgacag ttaaagtagg tattaatggt tttggacgta
    81841 ttggacgtct agcattccgt cgtatccaag atgtagatgg aatcgaagta gtagcaatca
    81901 acgacttgac agatgctaaa atgttggcac acttgttaaa atacgacaca actcaaggac
    81961 gtttcaacgg aacagttgaa gttcatgaag gttcattcaa cgtaaacgga aaagaagtta
    82021 aagtattagc taaccgcaac ccagaagaat taccatgggg cgaactaggc gtagatatcg
    82081 ttctagaatg tactggtttc ttcacttcta aatcagcagc tgaaaaacat ttaacagctg
    82141 gtgctaaacg cgttgttatc tctgctcctg gcggaaacga tgttccaaca atcgtttata
    82201 acacaaacca cgaaacatta actggtaaag aaacagttat ctcaggtgct tcatgtacta
    82261 ctaactgttt agctcctatg gctaaaacat tgaacgacaa atttggtgtt gttgaaggat
    82321 taatgacaac tatccacgct tacacaggtg accaaatgac tctagacgga cctcatccta
    82381 aaggtgactt ccgccgtgca cgcgctgctg ctgcaaacat cgttcctaac tcaacaggtg
    82441 ctgctaaagc tatcggttta gtaattcccg aattgaacgg taaattagac ggagctgctc
    82501 aacgtgttcc tgtaccaaca ggttcattaa cagaattagt aacagttctt gaaaaagaag
    82561 taactgttga cgaaatcaat gcagcaatga aagaagcttc aaacgaatct tacggataca
    82621 acacagacga aatcgtttct tcagatatcg ttggtatgac ttacggttca ttatttgatg
    82681 ctacacaaac taaagtaatg acagttggcg acaaacaatt agttaaaact gttgcttggt
    82741 acgacaacga aatgtcatac actgcacaat tagtacgtac tttagaatac ttcgctaact
    82801 tataagatag acaaacgtac aagtgtaaat tatagacatg tacatttaaa agcggggaag
    82861 caatcgcgct tcttccgctt tttttgataa taagtttatt caggaggaaa taaaatggct
    82921 aagaaaacag tgaaagatat cgatttaaaa gacaaaaaag ttcttgtccg tgttgacttc
    82981 aacgttcctt tgaaagacgg cgtgatcact gacgacactc gtatcaaagc agcattgcca
    83041 acaatcaact acgttctaga acaaggcgga aaagcaatcc ttttctctca ccttggacgt
    83101 gtaaaaacag aagaagataa agaaggaaaa tctttagctc cagttgcaaa acgtctaggc
    83161 gaattattag gtaaagaagt aacattcgta cctgaaactc gcggagaaca attagaagaa
    83221 gcaatccgca acatgaatga cggcgacgtt gtcgtattcg aaaatacacg atttgaagat
    83281 attgacggta aaaaagaaag caaaaatgat acagaactag gtaaatactg ggcttcatta
    83341 ggcgatgtat ttgttaacga tgctttcggt acagctcacc gtgcacatgc ttctaacgta
    83401 ggtatcgctt caactggtat cccaacagtt gctggattct tgatggaaaa agaaatcaag
    83461 ttcatcggtg aagcagtaga agaaccaaaa cgaccaatga ttgcaatcct tggtggcgca
    83521 aaagtttctg ataaaatcgg cgtaatcgaa aacttgattc ctaaagcaga taaaatcttg
    83581 atcggtggcg gaatgactta cacattctac gaagcaaaag gaatcaagat cggtaactct
    83641 ctagtagaag ctgataaagt ggaactagca aaagaattaa tcgaaaaagc aggcgacaaa
    83701 cttgtcttgc caatcgacaa tgtctgtgca ccagaatttt caaatgatgt tgaaacacaa
    83761 gtaatcgaag gagacattcc tgatggtttg atggctttag acatcggccc agcatcagta
    83821 aaattatttg ctgatacatt acaaggtgca aaaacagttg tttggaacgg acctatgggt
    83881 gtatttgaaa tgagtaactt tgctaaaggt acaatcggtg tttgtgaagc aatcgctaac
    83941 ttagaaggcg caacaacaat catcggtggc ggagactcag ccgcagcagc agaacaatta
    84001 ggatttgctg acaaattcac acacatctca acaggtggcg gcgcaagctt ggaattgctt
    84061 gaaggtaaag aattaccagg actagcagca atcaacgata aataaacgac caagtttgct
    84121 caattctgac gagtaatcga acaaaaggtg aagggagttt tcatgactcc ctagccattt
    84181 ttcaataaat taaggagtgt gttcctcaat gcgtaaacct attatcgcag gtaactggaa
    84241 aatgaacaaa actgcatcag aagcaaaaga atttgctgaa gcagtaaaaa acaaaatccc
    84301 ttcaaatgac aaagttgatt cagtcatcgg atctcctgca ttattcttac aagaattaat
    84361 gacatcagct gaaggtacag aactaaaaat ctcagcacaa aactgctact gggaaaactc
    84421 aggtgcgttc actggtgaaa cttcaccagc agctcttgct gatctaggtg ttcattatgt
    84481 gatcatcgga cattcagaac gtcgtgaata tttccatgaa acagacgaag acatcaataa
    84541 aaaagcgaaa gctatctttg caaatggtat gacaccaatc ctatgctgcg gcgaatcttt
    84601 agaaacctat gaagcaggca aaactgctga atggatcgaa ggacaaatta cagctggttt
    84661 agctgatctt tcagctgaac aggtgtcaaa catggttatt gcttatgaac caatctgggc
    84721 aatcggtact ggtaaatcag ctgacgcaca aatcgctgac gaaatttgtg gagttgtacg
    84781 tcaaacagtt gaaaaacttt atggaaaaga agtatcagaa gcagttcgta tccaatacgg
    84841 cggttctgta aaaccagaaa atattgctga atacatggca aaagaaaatg ttgacggtgc
    84901 tttagtcggt ggcgcaagct tgcaagcaga ttcattcttg tcattgttag acgctgtaaa
    84961 ataattgttg aaaatttgcg aaaaatgcaa gaaatgtttg cactaatgag caaaattaac
    85021 taaaatcgta tatgagggaa atcccttagg aaaaaacaaa ctcaaaggag agacaaaaca
    85081 tgtcaattat tactgatgtt tacgctcgcg agatcttaga ctcacgtggt aacccaacaa
    85141 tcgaagtaga agtatacaca gaaagcggag cttttggccg cggaatggtt ccttcaggtg
    85201 cttcaactgg tgaatacgaa gcagttgaat tacgtgacgg agacaaagct cgttatggcg
    85261 gtaaaggtgt aactaaagct gtcgacaacg taaacaacat cattgctgaa gcaatcattg
    85321 gctacgatgt tcgtgaccaa atggctatcg acaaagctat gatcgcttta gacggaactc
    85381 ctaacaaagg taaattaggt gcgaacgcta ttttaggtgt ttctatcgct gttgcacgtg
    85441 cagctgctga ctacctagaa gtacctttat accactacct aggcggattc aacacaaaag
    85501 tattgccaac accaatgatg aacatcatca acggcggatc acatgctgat aactcaatcg
    85561 acttccaaga attcatgatc atgcctgttg gcgctccaac attcaaagaa gctctacgta
    85621 tgggtgctga agtattccac gcattagctt caatcttgaa agctcgcgga ttagcaactt
    85681 ctgttggtga cgaaggtgga tttgctccaa accttggttc aaacgaagaa ggttttgaag
    85741 taatcatcga agcaatcgaa aaagctggct atgtacctgg taaagacgtt gttcttgcta
    85801 tggatgctgc ttcttcagaa ttctacgaca aagaaaaagg tgtttacgta ctagctgact
    85861 caggcgaagg cgaaaaaaca actgacgaaa tgatcaaatt ctacgaagaa ttagtttcta
    85921 aatatccaat catctcaatc gaagatggat tagacgaaaa cgactgggac ggattcaaga
    85981 aattaactga cgtattaggc gacaaagttc aattagttgg tgacgacttg ttcgtaacaa
    86041 acactcaaaa attatctgaa ggtatcgaaa aaggaattgc taactcaatc cttatcaaag
    86101 tgaaccaaat cggtacatta acagaaactt tcgaagctat cgaaatggct aaagaagctg
    86161 gctacacagc agttgtatct caccgttctg gtgaaacaga agattcaaca atctctgata
    86221 tcgctgttgc aacaaacgct ggccaaatca aaactggttc tctatcacgt actgaccgta
    86281 ttgctaaata caaccaatta ctacgtatcg aagaccaact tggtgaagtt gcagaataca
    86341 aaggtttgaa atctttctac aacttaaaaa acaaataatt cttttctaat gaagatacat
    86401 gatcgactat tgaaagatag tcgagtatga tcacttagtt aagtaatcga actctctact
    86461 tgctgaaata tcagcaggta gagagttttt ttgcaactag cttctgtata tgatgtttac
    86521 aaagaatggt acaagaaaag atcatccttg aaaaaggaaa gctaacaata actagcatca
    86581 tataagagct tttaaggata gaacagtaga ataggaatct atgggcaagt cctctcgtat
    86641 aaataaaata ggagggaagt agcagcatat ttttcataaa gtggctctct ttcttcattt
    86701 ttactttggt tgctcctatt tgaaaattcg agcacactca aatacaataa aaagaaatgg
    86761 caagggagag gattagatga aaaaagaaga aaaaatcgcg attttacaag agattatccg
    86821 aattaaatca gtgaatggaa acgaaggtga agtagcagcc tatttgaata agcttttggc
    86881 aaagcatgac ataacaggag agattgtttc ttacagagat ggtagagata atttgattgc
    86941 tcgttaccaa aagggccaat caggaaaagt gttaggccta tctggacata tggatgtcgt
    87001 ggcggctggt gatgaatcgt cttggaccta tgcacccttt gcagcagaaa tccatgggaa
    87061 ccgtttgtac ggacgtggtg ctacagatat gaagagtggc ttggcagcaa tggtgattgc
    87121 catgatcgaa ctgaaagagt caggaaaacc atttaacgga acagtcaaat tattagccac
    87181 tgtcggagaa gaagtgggtg aactaggtgg ggaacagctc acgaaagcag gctatgtaga
    87241 tgacttagat gccttgatca tcggagagcc aaccaattac agtttgatgt atacgcacat
    87301 gggatcaatc aactatacag tcacctcgca cggaaaagaa gcgcatagct ctatgccaga
    87361 ccaaggttat aatgcaatca atcacctaaa tgaattcatt acgaaagcaa atgctgaaat
    87421 gaatcacctc gctgaaacaa tcgagaatcc tgtcctagga aaaacaatcc ataatgttac
    87481 attgatcagt ggcgggaatc aagtcaacag catcccaagc catgcgcagc tccaagggaa
    87541 cattcgatcg attccagaat acccaaatga taaaatcatt gcattgctgc aatcaatcgt
    87601 caatgaattg aatcaagaga cagactatca tttggaattg atgatcgatt acaacaaaat
    87661 acctgtcaaa gctgatccag attctccttt gattcattgc attcagcaac aattcagcca
    87721 gccattacct ttagttggag cggcagcaac aacggatgca gccgaattta cgaaagctaa
    87781 ccattccttc gactttgtcg tctttgggcc tggtgtcgtt actttgcctc atcaagtaga
    87841 tgagtatgtt gaaatcgata attatttgga tatgatcgaa aaatatcaag gaattatttt
    87901 atcctactta gcgtaagatt tttttaat
//
LOCUS       DAJSBN010000003        87389 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJSBN010000003 DAJSBN010000000
VERSION     DAJSBN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.003"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(66..1544)
                     /locus_tag="ONT86_000212"
     CDS             complement(66..1544)
                     /locus_tag="ONT86_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2351037.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1842..2777)
                     /gene="murB"
                     /locus_tag="ONT86_000213"
     CDS             complement(1842..2777)
                     /gene="murB"
                     /locus_tag="ONT86_000213"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR2351038.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2850..3770)
                     /locus_tag="ONT86_000214"
     CDS             complement(2850..3770)
                     /locus_tag="ONT86_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2351039.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3883..4635
                     /gene="xth"
                     /locus_tag="ONT86_000215"
     CDS             3883..4635
                     /gene="xth"
                     /locus_tag="ONT86_000215"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR2351040.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5499..6032
                     /locus_tag="ONT86_000216"
     CDS             5499..6032
                     /locus_tag="ONT86_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR2351041.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6522..7046)
                     /locus_tag="ONT86_000217"
     CDS             complement(6522..7046)
                     /locus_tag="ONT86_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2351042.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(7039..7512)
                     /gene="tsaE"
                     /locus_tag="ONT86_000218"
     CDS             complement(7039..7512)
                     /gene="tsaE"
                     /locus_tag="ONT86_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR2351043.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7635..8615)
                     /gene="pta"
                     /locus_tag="ONT86_000219"
     CDS             complement(7635..8615)
                     /gene="pta"
                     /locus_tag="ONT86_000219"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR2351044.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8768..9448)
                     /locus_tag="ONT86_000220"
     CDS             complement(8768..9448)
                     /locus_tag="ONT86_000220"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR2351045.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9574..10443
                     /locus_tag="ONT86_000221"
     CDS             9574..10443
                     /locus_tag="ONT86_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2351046.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10469..10984
                     /locus_tag="ONT86_000222"
     CDS             10469..10984
                     /locus_tag="ONT86_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351047.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(11031..11624)
                     /locus_tag="ONT86_000223"
     CDS             complement(11031..11624)
                     /locus_tag="ONT86_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2351048.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11649..11828)
                     /locus_tag="ONT86_000224"
     CDS             complement(11649..11828)
                     /locus_tag="ONT86_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351049.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11905..12414)
                     /locus_tag="ONT86_000225"
     CDS             complement(11905..12414)
                     /locus_tag="ONT86_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351050.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12510..12890)
                     /locus_tag="ONT86_000226"
     CDS             complement(12510..12890)
                     /locus_tag="ONT86_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2351051.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            13220..13969
                     /locus_tag="ONT86_000227"
     CDS             13220..13969
                     /locus_tag="ONT86_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCR2351052.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(14153..15430)
                     /locus_tag="ONT86_000228"
     CDS             complement(14153..15430)
                     /locus_tag="ONT86_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351053.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15509..16153)
                     /gene="pcp"
                     /locus_tag="ONT86_000229"
     CDS             complement(15509..16153)
                     /gene="pcp"
                     /locus_tag="ONT86_000229"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCR2351054.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16352..17296)
                     /locus_tag="ONT86_000230"
     CDS             complement(16352..17296)
                     /locus_tag="ONT86_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCR2351055.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17298..17984)
                     /locus_tag="ONT86_000231"
     CDS             complement(17298..17984)
                     /locus_tag="ONT86_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCR2351056.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGIASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            18458..18778
                     /locus_tag="ONT86_000232"
     CDS             18458..18778
                     /locus_tag="ONT86_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCR2351057.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            18932..19837
                     /locus_tag="ONT86_000233"
     CDS             18932..19837
                     /locus_tag="ONT86_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2351058.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            19834..20607
                     /locus_tag="ONT86_000234"
     CDS             19834..20607
                     /locus_tag="ONT86_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR2351059.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(20788..22212)
                     /locus_tag="ONT86_000235"
     CDS             complement(20788..22212)
                     /locus_tag="ONT86_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2351060.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            22423..22869
                     /gene="nrdI"
                     /locus_tag="ONT86_000236"
     CDS             22423..22869
                     /gene="nrdI"
                     /locus_tag="ONT86_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR2351061.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(22934..24916)
                     /locus_tag="ONT86_000237"
     CDS             complement(22934..24916)
                     /locus_tag="ONT86_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2351062.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(24930..25826)
                     /gene="murQ"
                     /locus_tag="ONT86_000238"
     CDS             complement(24930..25826)
                     /gene="murQ"
                     /locus_tag="ONT86_000238"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR2351063.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(25829..26890)
                     /locus_tag="ONT86_000239"
     CDS             complement(25829..26890)
                     /locus_tag="ONT86_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR2351064.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(27101..27667)
                     /locus_tag="ONT86_000240"
     CDS             complement(27101..27667)
                     /locus_tag="ONT86_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2351065.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(27695..29197)
                     /gene="eat(A)"
                     /locus_tag="ONT86_000241"
     CDS             complement(27695..29197)
                     /gene="eat(A)"
                     /locus_tag="ONT86_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR2351066.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(29455..30576)
                     /locus_tag="ONT86_000242"
     CDS             complement(29455..30576)
                     /locus_tag="ONT86_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR2351067.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            30702..31643
                     /locus_tag="ONT86_000243"
     CDS             30702..31643
                     /locus_tag="ONT86_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR2351068.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(31793..32689)
                     /locus_tag="ONT86_000244"
     CDS             complement(31793..32689)
                     /locus_tag="ONT86_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR2351069.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            32958..34298
                     /locus_tag="ONT86_000245"
     CDS             32958..34298
                     /locus_tag="ONT86_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2351070.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            34516..34959
                     /locus_tag="ONT86_000246"
     CDS             34516..34959
                     /locus_tag="ONT86_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCR2351071.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(35463..36881)
                     /gene="nhaC"
                     /locus_tag="ONT86_000247"
     CDS             complement(35463..36881)
                     /gene="nhaC"
                     /locus_tag="ONT86_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR2351072.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(37284..38507)
                     /locus_tag="ONT86_000248"
     CDS             complement(37284..38507)
                     /locus_tag="ONT86_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCR2351073.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(38538..39377)
                     /locus_tag="ONT86_000249"
     CDS             complement(38538..39377)
                     /locus_tag="ONT86_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR2351074.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(39394..40074)
                     /locus_tag="ONT86_000250"
     CDS             complement(39394..40074)
                     /locus_tag="ONT86_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2351075.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(40076..41149)
                     /locus_tag="ONT86_000251"
     CDS             complement(40076..41149)
                     /locus_tag="ONT86_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2351076.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(41205..41443)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(41486..41719)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(41796..42467)
                     /locus_tag="ONT86_000252"
     CDS             complement(41796..42467)
                     /locus_tag="ONT86_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2351077.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(42554..45232)
                     /gene="mgtA"
                     /locus_tag="ONT86_000253"
     CDS             complement(42554..45232)
                     /gene="mgtA"
                     /locus_tag="ONT86_000253"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR2351078.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            45891..46328
                     /gene="ndk"
                     /locus_tag="ONT86_000254"
     CDS             45891..46328
                     /gene="ndk"
                     /locus_tag="ONT86_000254"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR2351079.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(46350..47216)
                     /locus_tag="ONT86_000255"
     CDS             complement(46350..47216)
                     /locus_tag="ONT86_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2351080.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(47376..49469)
                     /locus_tag="ONT86_000256"
     CDS             complement(47376..49469)
                     /locus_tag="ONT86_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR2351081.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(50218..50841)
                     /locus_tag="ONT86_000257"
     CDS             complement(50218..50841)
                     /locus_tag="ONT86_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351082.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(51000..52397)
                     /locus_tag="ONT86_000258"
     CDS             complement(51000..52397)
                     /locus_tag="ONT86_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2351083.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(52429..53400)
                     /locus_tag="ONT86_000259"
     CDS             complement(52429..53400)
                     /locus_tag="ONT86_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2351084.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            53774..54199
                     /locus_tag="ONT86_000260"
     CDS             53774..54199
                     /locus_tag="ONT86_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351085.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(54687..55388)
                     /locus_tag="ONT86_000261"
     CDS             complement(54687..55388)
                     /locus_tag="ONT86_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR2351086.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(55404..55844)
                     /locus_tag="ONT86_000262"
     CDS             complement(55404..55844)
                     /locus_tag="ONT86_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR2351087.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(55844..56419)
                     /locus_tag="ONT86_000263"
     CDS             complement(55844..56419)
                     /locus_tag="ONT86_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCR2351088.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(57267..57746)
                     /locus_tag="ONT86_000264"
                     /pseudo
     CDS             complement(57267..57746)
                     /locus_tag="ONT86_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(57905..58606)
                     /locus_tag="ONT86_000265"
     CDS             complement(57905..58606)
                     /locus_tag="ONT86_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2351089.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(58714..60123)
                     /locus_tag="ONT86_000266"
     CDS             complement(58714..60123)
                     /locus_tag="ONT86_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR2351090.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(60171..62036)
                     /locus_tag="ONT86_000267"
     CDS             complement(60171..62036)
                     /locus_tag="ONT86_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2351091.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(62157..63008)
                     /locus_tag="ONT86_000268"
     CDS             complement(62157..63008)
                     /locus_tag="ONT86_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2351092.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(63318..64031)
                     /locus_tag="ONT86_000269"
     CDS             complement(63318..64031)
                     /locus_tag="ONT86_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR2351093.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(64099..64425)
                     /locus_tag="ONT86_000270"
     CDS             complement(64099..64425)
                     /locus_tag="ONT86_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR2351094.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(64548..64775)
                     /locus_tag="ONT86_000271"
     CDS             complement(64548..64775)
                     /locus_tag="ONT86_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR2351095.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(65097..66350)
                     /locus_tag="ONT86_000272"
     CDS             complement(65097..66350)
                     /locus_tag="ONT86_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR2351096.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            66411..67151
                     /locus_tag="ONT86_000273"
     CDS             66411..67151
                     /locus_tag="ONT86_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2351097.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(67237..67995)
                     /locus_tag="ONT86_000274"
     CDS             complement(67237..67995)
                     /locus_tag="ONT86_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR2351098.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(67992..69020)
                     /locus_tag="ONT86_000275"
                     /pseudo
     CDS             complement(67992..69020)
                     /locus_tag="ONT86_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(69040..72207)
                     /locus_tag="ONT86_000276"
     CDS             complement(69040..72207)
                     /locus_tag="ONT86_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351099.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(72431..73315)
                     /locus_tag="ONT86_000277"
     CDS             complement(72431..73315)
                     /locus_tag="ONT86_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR2351100.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(73410..73847)
                     /locus_tag="ONT86_000278"
     CDS             complement(73410..73847)
                     /locus_tag="ONT86_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351101.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(74064..74975)
                     /locus_tag="ONT86_000279"
     CDS             complement(74064..74975)
                     /locus_tag="ONT86_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR2351102.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(75432..75860)
                     /locus_tag="ONT86_000280"
     CDS             complement(75432..75860)
                     /locus_tag="ONT86_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351103.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(75888..76526)
                     /locus_tag="ONT86_000281"
     CDS             complement(75888..76526)
                     /locus_tag="ONT86_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2351104.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(76530..78512)
                     /locus_tag="ONT86_000282"
     CDS             complement(76530..78512)
                     /locus_tag="ONT86_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCR2351105.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            78692..79357
                     /locus_tag="ONT86_000283"
     CDS             78692..79357
                     /locus_tag="ONT86_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2351106.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(81292..84441)
                     /locus_tag="ONT86_000284"
     CDS             complement(81292..84441)
                     /locus_tag="ONT86_000284"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HCR2351107.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(84454..85671)
                     /locus_tag="ONT86_000285"
     CDS             complement(84454..85671)
                     /locus_tag="ONT86_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR2351108.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(85661..87256)
                     /locus_tag="ONT86_000286"
     CDS             complement(85661..87256)
                     /locus_tag="ONT86_000286"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR2351109.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT        25389 a        18046 c        14849 g        29105 t
ORIGIN      
        1 cttttctttg aaaaaaacat aaatcagcta ataaatatgt gagacaaagc tgccatccat
       61 aaatactagc tgattttttc agttaaatgt ctattttttt ctctgatgat ggtatttcca
      121 aaatattccc agtcatctgg agtcggtgga tttttccaaa ttatctgttc tttattaaat
      181 ttttcaagta aacaatcaga taaataaata ttcgtacgag aagtgattcg actctcaatc
      241 acaatattgt aatttttaaa tcctttcact tgagaaataa tgtaatcggt ataagcagat
      301 ccttgcgaaa aatcaataca gacatgaatc ggtgcttcaa aatatgatac cggacaaata
      361 ccgaccaata aaaagagata atcataaaat aagcgaatca tgaaacgttc atcttttccc
      421 aagaaatagc gtttattctc ttgtatgacc ttttggattg ctgtactgaa tattggatac
      481 gtctcgaaga aaaactgtag ctgcttttta gacgtaaagc tagtcatctc aaagtcgaat
      541 atccaatttt ttcgattgac atgcatcata cccatacgaa gttcttttcg tatatatagt
      601 ctttcttctt cagagatttc aatcgaaaat ggtaattgat caatcaattc cactgacatt
      661 ttttgactaa tcatgtggat atcatgaaaa tgagcttccg caagtccaaa tgcgccatct
      721 aattcaaatt cttcttccgt gatcaaaaac aacatgatat aagaaatctc atcaatgatc
      781 tgctctttag ggatatgaag aaaagaaata tgtgataaat ccttgatgat cttcattcct
      841 tctgagctct taaagtattc atctgaaaag aaacgttttc cgattggttg attcccatag
      901 gccctcaaca aactaatagt ggttagcaaa ttgaatttga ttcgttttgt tgcagacagt
      961 tttaaattga acagatattc aaaatattgc acgatactct ttgcttcatt catgacgctt
     1021 tctctaaatg gcatagaaaa accattaaag accccgaaat ataccccaaa taatacgttc
     1081 cgtatgttta tttcttcccc aactaatatg tttttcttta actttatttg atgctgttca
     1141 agaaaagaga ctaattctct tctttttaca taggctcttg atatgcttaa aaaatgattc
     1201 tctgcaaatt tttcgatcgt tacttttgaa tccacgatct ccgttaacag tcgagcaatt
     1261 tttgatcgtt gaaagtagat tcttcttaaa tttttgatat ccgtcaaatt cagacgttcg
     1321 acatggatct ctgcttcttc tgaaatttgg atctctggct ttgttttgaa ttcagtaaat
     1381 tctttattta attctatgat ataatttctt gttttaaatt tagacaaacc taatgtttca
     1441 tataacttag gcagcactac ataattcttt ttctcaaatt ctaaatatct agctagcagc
     1501 agtttttctc tatcttcctc ttctataagg taatccaact tcatatccta cacctcaaaa
     1561 aatgcctcat accaattact tgatatgcag atgaaagttc atcttttcac ctatactgat
     1621 tttatgatgg attatgcttg ttttttcatc agttattctc attttttatc ttttttttga
     1681 gaatattgca aaaaatggaa aaatttatga ggaaatattc tatatttttt gttttattgt
     1741 gttaaaatgt acaagatttg cgccatttgc gtaaacaacc acaaaaaaga gcaggacggc
     1801 caccgtcctg ctcttttact tattttaagt tccattactt attaattggc taaacttctg
     1861 cttgtttctg atattgcttg tttttctcca atgatccgca cttctgtttc taaagacaca
     1921 tcaaattttt ctttgattac ttcttggatg tgcgcaatca attcaatata atctgtcgct
     1981 gtcgcatgat cgatattgac gatgaatcca gcatgttttt cagagacttg tgcaccgccc
     2041 catttcaggc cttgcaatcc tgcttgttgg atcagctgac ctgtatagtg tcctactggg
     2101 cgcttaaaga cacttccgca tgaagggtat tctaatggct gttttgattg acgcaattcg
     2161 gtcaactcat ccattcttgc tttgattgct tcataatccc ctgtttgcaa actgaatcga
     2221 gcttccaata cgatagcacg taattcttgg actttactgt gacgataaga aaataccatt
     2281 tcgtcttttg tcaaagtttt caatgttcca tcttcaagca aaagatccac ttcagcaaaa
     2341 acatctttga tctctccgcc atacgcacct gcattcatat aaaccgctcc accgacactt
     2401 cccgggatgc cgcaagcaaa ttcaaatcca gtcaatgatt ctgccaaagc tgcataagtc
     2461 gtatcaatca gtgaggcacc tgcttccgca atgaccatcg ttcctttgac atgaatctgt
     2521 ttcatttcag tcagcatgat gactacaccg cgaatcccgc catcttgaac gatcagattg
     2581 cttgcatttc ctaataccat ccaaggtata tcatggattc ggcaataatc gatgatttgc
     2641 tttacttctt cttgtttttt cgggaatgca aggacatcag ctggtccacc tgttttcgta
     2701 aacgtatagt tcatcaatgg ttcatcaaac aatatagtaa tcgtggctaa gctttcaact
     2761 atttcttctt tattcactga ggattttcct ttcttttctc aaacgtcctc attattctat
     2821 cacgcttaat acaatcttgc cagtcttgtt caaagttgat gacggtagtg atctttaagc
     2881 acttcctcac ataattcatg acttcttaca acttcttttt gtttaagcaa ttctgtttcc
     2941 gatttaccta aacggatctg agaaaagaag gcctctacca ttggctcaaa cccgcgtttg
     3001 actagcgtgt ttgtccagtc gccaaattct ttcacttttt ttccatcagc ctcttgaatg
     3061 acaagctggg tcagatcgtt tacttcataa gtaccatttt tacttgttac ttgatatttt
     3121 tctatattag cgccacttgc taaatccatc gttaaaatag ccgtttgatt ttgtgtttct
     3181 aattgtaaaa tggcatattc caaaaattcc tttgtttctc ttatctggta ttttgttctc
     3241 aaaatgggtt catctaataa atacacagct gtatcaataa gatgtaaaaa caaatcataa
     3301 aggacgaact ctgtcgtttc ttttgccgct attcgatttt tttgcaggtg gatcaaccgc
     3361 ttatctggga tacgtttcaa ttcttctacc ataggagcaa agcgccggtt gaaaccgatc
     3421 atcaaaatta tttggttctt atcagctaat tcttgcaatt cacgaacttc atttagatgg
     3481 acactcagtg gtttgtcaat ataaacatgg ataccatttt ctagacattt tttggctaat
     3541 tcataatgta cttttgttgc tgcgtggatc atacatgctt cgattttttc ttctaaaagc
     3601 tcatcgatcg tttcatatag atgttggaac ccgtactctt tttgaattct ttcttttgtt
     3661 tctttatttc ttgttgcaaa ataaaaatca agtgttcctt gttttttcgc ataagttggt
     3721 aaatatgctt tttctgcgat attccctaca ccgattacac caatcttcat ctactttcct
     3781 cctggatcat ttagttactt tcatcttaca ccatttttta ctaaaatgag gtacaatagt
     3841 atcgcaaagg taaggaaatt aattcgaagg agtgtgcttt tattgaaatt tatctcttgg
     3901 aacgtaaacg ggcttcgtgc catcgtaaat aaaaattttt tagaagtttt tcatgagtta
     3961 gacgccgatt ttttctgtct gcaggaaacg aaactccaag caggacaaat tgatttagat
     4021 cttccaggtt attatcaata ttggaattac gcggaacgaa aaggctattc cggcacagcg
     4081 atttttgcta aaaaaccagc attgaatgcc acttacggaa tgggtatcga tatccatgat
     4141 acagaaggcc gtctgattac tttggaatat tccgatttct ttcttgtaac ctgttacacg
     4201 ccaaattctc aaagcgaatt aaaacgttta gattaccgat tagaatggga agaagccttt
     4261 tataattatt tggaaaatct aaaaaaacaa aaaccagtca tcgtgtgtgg ggatctaaat
     4321 gtcgctcatc aaaagattga tttgaaaaac tggaaaacga atcaaaaaaa tgctggattc
     4381 actccagaag aaagagcggc tctttctcga ctgttggata acgggtttat cgatacattc
     4441 cgttattttt atccaaccca ggaaggtgtc tactcttggt ggaactaccg attcaattca
     4501 aggaaaaaca atgctggttg gcggatcgat tactttttga cgagcaaaga tttagagcca
     4561 ggactagctg atgcaaagat ccatacggac attatgggca gtgatcattg tccagtagaa
     4621 ttagatttga aataaagagg ttgtgaaaaa gtcgtttagt ttcgagtatt aagaaaaatt
     4681 ttgtgaaaat ggctttccac attttgcata aatttggcat aataccgaga aactggcttt
     4741 tgaacactgt ttatcgagag attgtgaaaa agctgtcctg catcaagtaa taagaacagc
     4801 caaacaaaaa tagttcctca tatttttcgt ttggttgggc ttattaccgc agatacaagc
     4861 ttttgaacac cgtttattaa gaggttgtga aaaagctgca ctgcatcaag taataagaac
     4921 agccaaacaa aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagatgca
     4981 agcttttgaa caccgtttat taagaggttg tgaaaaagct gcactgcatc aagtaataag
     5041 aacagccaaa caaaaatagt tcctcatatt tttcgtttgg ttggacttat taccgcagat
     5101 gcaagctttt gaacaccgtt tattgagaag ttgtgaaatc agcgtcctga cctgagtatt
     5161 agagaaacaa atggcaaaat agttcctcat attttgctat tttttgagct aataccgagg
     5221 gtcagctgat tgaacaccgt ttagtggaga agttgtgaaa aagtcgccta ctttcgagtc
     5281 gtaagaaatt tttttgaaaa tggttcctca caactttgca taaatttgac ttaataccga
     5341 gaagtcatga tccccatcta actccaaatc acaaggaatg agttcaaaaa ctagcttttc
     5401 tcaacaaata ttacataaaa aattctatgt tttttgttaa atttatttgc ttttttcaaa
     5461 agtatgttac agtaatttcc caaggaggtt ttacagaaat gaattttgtt gccatggatt
     5521 ttgaaactgc caatcatcag ccttatagtg cttgttctct tgcgctagtc atggtaaaaa
     5581 atagccagat cgttgatgaa ttctatacat tgatacagcc tgagactcct tttttctggc
     5641 gaaatgtcca aattcacgga atccatcaag aagacgtacg caatgcgccg aaattcccag
     5701 aagtctggca aactatccaa ccatattttc aaaaaaatcg tttagtcgtt gcacataatg
     5761 ctgcttttga tacaaaggtc ttagcgggat gcttagatta ttatcatctt gaacagccta
     5821 attatctttc tttatgtaca gtgaaaagta gccgtcgcct gtttcctgaa tttccaaatc
     5881 atcgattgaa cactgtgtgc gaaaatttga acatccaatt agatcatcac catgatgcat
     5941 tggaagatag tcgtgcatgt gcggaaatcc tgctttatca agaaaaaaaa ttcgggacag
     6001 atccattgaa aaaacttgtg actataaagt aaaagaatac ttatgaaaca cacaaagagg
     6061 tcgtgagcct gccgtttcac tccgagtcac aaggagcaat ttcaaaaaat agcttctcat
     6121 gtttattaaa tattgtgact ggtgaccgag gagctggcag gagaacaccg tttattggag
     6181 aggttgtgaa aaagctgttc tgcatcaagt aataagaaca gccaaacaaa aatagcttct
     6241 catatttttc atttggttgg acttattacc gcagatgcaa gcttttggac accgtttacc
     6301 aagaggttgt gaaatcagcg ttttgacctg agtattagag aaacaaacgg caaaatagtt
     6361 cctcatattt tgccgttttt tgagctaata ccgaaggtca gctgattgaa caccgtttat
     6421 tcaaaaaaaa tggactgcga cacgactttt gtcacagtcc cttcgtgtgt ttttctgcct
     6481 tctcaaaagg atgacagttt ctcactcttt tcctatttct tctattcaat caaataactc
     6541 attttatata catctaaaaa ttcacctaga tctgttcttg cccctctagg catgaccgct
     6601 tcgatttgga agcccatttt tcggtaaagg tgtacggctc tttcattacg aacttgaacg
     6661 tcaagctcca atcgaaaaag tacacccatt tccttcgccc aactgatgat ttcttctagc
     6721 ataagagtgc ctagtcccat tccccaatat tcctgcagaa tactgatacc gacttctccc
     6781 acgtgagaca aacgaaattg actgtctgcc ttgacagatg ctgttccaat aatcgtccct
     6841 tcatagattg caagcagcaa taaattatta ttgctttcgt atatataatc caactcttct
     6901 tctaaggttt ctggtggtaa caggagttct gcttcatcca aaactaaaaa ttctgtctcc
     6961 ttattaactt gtcccatcat caaaagaatg tcttttgcat ctcctggcac tgcttcccgc
     7021 aagatgatcc caacttcttc actcatgatt ttcagcccat ttttctagaa ttcgctgttt
     7081 gaaggtttca ctttgctccc cgtatgagat tagtttcaca taccgctttt ctaaatctgt
     7141 atcacttttt tctaaaatca gttccaaata atcctccggc agtgcttctt ctaatagatt
     7201 tccccattca acaacggaaa gcccatcacc ttcaaaatat tcatctaacc ctaaatctgc
     7261 tccgctagct gctacccgat aaatatccat atgatacaac ggaatccgac cttgactata
     7321 ttctctaata atcgtgtatg tcggactttt gatcatttgc gttatcccta atccttgggc
     7381 aattcctttc gtcaacgttg ttttacccgc acctagatca ccagtcaata tcagattgtc
     7441 acctggcatt gcggaatgac caattgcctc tgcgaattgt tctgtcattg ttaatcctga
     7501 tagttcgatc atttttcatc ttctcccgct cttctttctt tttgccagta tatcgcgtaa
     7561 tgaagttttt ttaaagaaaa aatgaactag tcaagaacag cagctgctgt ttttggctag
     7621 ttcgcgtgta tttgttaatc aatcaatgtt tgtgctgctg tgataatcga taatttatat
     7681 acatcttctt catttgctcc acgagacaaa tcagaaaccg gtttgttcaa gccttgtaga
     7741 attggtccga ttgcttcaaa gttaccgaaa cgctgagcaa ttttatagcc gatatttcct
     7801 gactgcaatt ctggaaatac aaagactgtt gcttgaccag ctacttctga atctggcgct
     7861 ttcagttgtg caactgaagg aacataagaa gcatcaaatt gcaattcgcc gtcgatcatg
     7921 tattgtggcg ctagtttttt agcgatttta gttgcttcaa ctactttgtc tacttctggc
     7981 gatttcgctg agccttttgt cgagaagctc atcattgcta cttttggttc gatatcgaac
     8041 aattctgctg tttttgcaga atctacggcg atttcagcca attcttgcgc agttggattc
     8101 acgttgatcg cacaatccga aaagacatat ttttcttgat ctcttccacg taccatgatc
     8161 atcgctccgc ttgtccgact gatgccaggc tttgttttga tgatctgaag tgctgggcga
     8221 acagtgtcgc cagtcgaatg gatcgctcca gagaccattc cgtctgcaat ccccatatac
     8281 gtaagcatcg taccaaagta attcacatct tttaaaatct tttctgcttg ttctttggat
     8341 gcctttccat ttctacgttc aacaaaagta tccaccattt catcccactt atcgtaatta
     8401 tcagggctga tgatcgtaaa gctagatgtt ttgattcctc tggcatgcgc tgcatttctt
     8461 atttcttctt cgttccctac taaaatgggt tccacaagtt cttctgcttt caaccgggca
     8521 gctgcgccta aaatacgagc atcagtagct tccgggaaaa cgattttgat cccacggcga
     8581 acaattttaa atcttaaact atcaaacaat tccacaagat gacctccaga cgtttttata
     8641 tgtattatca tacactattc cagaaaaaaa gaacagttta ttttatctgt atcagtcaaa
     8701 aatgctggaa aaattaagtt tttcacatac aaagcgaact gcactataac aggaaagcgc
     8761 tgttttatta cactgtttca ggcagttgcc aatcaatcgg ttcttctcct aacgcaagga
     8821 gcgcatcatt cgttttcgag aagggtttgg aaccaaaaaa gccacgactt gcagataacg
     8881 gactgggatg tggagatgtt atgatgatat ggcgactctt atcgatcatt ttgatttttt
     8941 cttgtgccgg ttttccccac aagataaaca cgattggttt ttcccgttca tttaattttt
     9001 caatgatcgt gtctgttaat ctttcccaac ctttcccgcg atgagaatac gcttgccctt
     9061 cccgaacggt aagaacggtg ttgagtaaaa ggactccctg tttagcccat gacactagat
     9121 ttccatgttg aacaggtgcg attccaagat catcataaag ctctttatag atatttctta
     9181 acgatggcgg gatctttacg ccaggttgca cagaaaaaga taagccatga gcttgatttt
     9241 ctccatgata aggatcttgt cctaaaatca ccactttaac ttcttcataa ggagtcaatt
     9301 ccaatgcttc atagatatga tacatatctg gatggatctt ttgggacgca tattcttttt
     9361 ttaaaaattc tcttagatgc agataataat cttttgaaaa ttctgaggac aaaacttctt
     9421 gccaactgtt atgaatgatt tctttcaaaa actccacctc ctaataaaaa gaataactca
     9481 cattttcttc aatcacaagg gacaatcttc accctcagca cttgattaat atggtaaact
     9541 aggaacaaga agcagaaagg aacgataatc attatgatca aattaattgc atcagatatg
     9601 gacggcacat tattagatgc ccatatgagt gtttctcctg aaaatgccga ggcgatacgt
     9661 tttgcaaatg atgccggtgt tgagttcatg gtcgctactg gtcgaaacta tcaagaagct
     9721 cgcgcagctt tagatgaggt aggaatcgat tgtgcgatga tcactttgaa cggggcacag
     9781 gtatttgata aagaaggaaa ttcattgttt accgtgtcga ttccaaataa tcaagcaatc
     9841 tctgttttag atattctaga tgctaacggt atttactatg aagtagcaac caacgatggt
     9901 ctttattctg aaaatcaagc gaaacgaatt gaaagttttg cttccatggt ggctacccat
     9961 ttacctcacc ttacgtataa aatggcgatt gccatggcct cagctaagct ggagttattg
    10021 cacatcacat acgtggacag tattcgtgct attttggaag aaaagaatct ggaagtttta
    10081 aaaattattt gttttcatac agaagggcca gctgtattag gtccagttgg aaagcagatt
    10141 tctcttttag gcgatctagc cgttacttcc tctggtcaaa acaatatcga agtgaaccat
    10201 aaaaacgctc aaaaaggaat tgctgttgcc catgtcgctc acgagcgtgg gatttcttta
    10261 gaagaagtca tgacgattgg cgataacttc aatgatgtca gcatgctgca agtagcagga
    10321 gtcagtttcg caatgggaaa tgcagaaatc gaagtaaaag attatgccaa atatacaacg
    10381 gacacaaacc ttgaatctgg cgtaggaaaa gccatattgc gcgcgattgc agaagacttg
    10441 tagttttatc tagaaaagag ggatatagat gtcagaattt ttcattcagg aaaagcagct
    10501 tggacgtgca actcggacaa ttgtcaaaaa cgaaaaagga cattccctgt ttttgatggt
    10561 tggtcgctgg ggaacaaagg gcgatgcatt gtctctttat gccatgaatg gcgatctcgt
    10621 tgctcacatc aagcagataa gcctgacatt cggtacgcgt tttgagctgt ataaagagtt
    10681 cgaaaaggtg ggaaccatgc agaaaatatt caattggccc ggtgactttt attatattcg
    10741 tagacttcac tggacagtcc aaggtgatat ctataatcat caatatcaaa tccatcattt
    10801 caatcagcaa atcatgaaaa tggataaagc aacacttttt tcaggagatt actatgtatt
    10861 ggatgtctca aatgatgaag atgctccagt ttgtatttgt attgctgcgg ttttagatta
    10921 ctggctctac cgtaaagaca aaagtcgcga taagcctgtt tcttaccgac ttaacttaag
    10981 ttaaaaagat gtacctgaaa tgcggaaata cgcatttcag gtacatcttt ttagatattc
    11041 ttttgcatat gatcataaag atgaccactg atcatccgct gaccgacaac tttgaagtca
    11101 tggcgttcgt ataatttttt agcctttgga tttttttcat ccacacttag accgatgacg
    11161 gagcgattag ctttcttcgc caatttaggt aatgcttcta ataatcgcga accgatgcct
    11221 tgtcctctaa aatcttctga tacactgata gagtccaaat accattcgtt gggaaacgtt
    11281 tctttatcaa tgaacaaacg tgtagacgca tctagctgat atttctctaa gataggtacc
    11341 agtggctgat cgatgattga ttcttcttca tctgtataac cgtaagcaac tcctgcaact
    11401 ttaccgtcta tttcttctac gatacctcgt gaataactgt aacgataatt tggatcagtg
    11461 attgcttctt ctaaaatctc taaagtcttt tgttcccctg tctgcttcaa aaatggcagt
    11521 tccatatctt ttagaatcac taagatcaat ggggcgattt ctggagcatc ttcttttctc
    11581 gcaaaacgaa tcatagtttc ctctcctttt ctccattcta acatgcagaa aaagaaatcg
    11641 caaaatcgtt attttaaaaa atcttcaatc ttttcagtaa tgaaataacc atgagtaacg
    11701 attcttgccg cttcagttcg gcaataaggg cagacgactg gcatttcttt atcattatgt
    11761 tcgactacat tattgacttc ttcgtatgca aatttttttc cgcaattagg gcatttcatt
    11821 ttttccatgt tatctcaact tcctttcatc tttactctct tagcttactc ttatattgta
    11881 aaaaagagaa gcaatatcct tcttctatgc agatatccat acctgacggt cctgttctcc
    11941 atctattaat cggactttat aataaggacg ttcatctttg actagaagtg tccactcttt
    12001 cacttttgct tttttcccgt agtagctgac tgcttccttc atcgctgctt gcggttgttt
    12061 gaactcttcc gttttttcta tatcaaaaag atgttctgcg gccttttccg tgtcatttcg
    12121 tacggttgtt ttcccagtgg atggattgac tgctatgact tgtccttcac aaaaaaactg
    12181 gtattcatat gttgcattcg taggattctc caaatctttt tcaactgcaa aatgaatagc
    12241 agatatgtta tgttcttttg tttcgttttt tacgattgct gctgcttttt tcaatgagat
    12301 ccgatattcc tggtcactaa tatcatccat gtgaaaagcg gcattgagca gtcccgtcat
    12361 accggtaatc cctattacga tgagggcagc aacaccaaac atcatttttt tcattcttca
    12421 ttctcctgtt tctttttttt aaagaatatc aagaaatgaa cgaatgaaaa atcgaatgaa
    12481 acgaaaaaaa agtgacaatt ttgttatctt tattcgtgca gttctaatgg caatccgtct
    12541 ggatcaaaga aaaacgtcat tttttttcct gtaaaggtat cttctctaat cggttctgat
    12601 tcgatcccta aggactgcaa ttctgcaatt gtttcttcaa tatcgtctac tttgaaagca
    12661 agatgacgca agccacaagc ttctggatag tttggtcgtt gtggcgcatt agcaattcca
    12721 aaaatctcca attcagcact tcctagtttc agatctaatt tatagtcatc tcgttctttg
    12781 cggtaatttt ctctgattac ttcaaatcct aatttattaa cataaaaatc acgtgatttc
    12841 tgataatctg aaacaatgat ggctatatga tggatcgttg taaggttcat atgagcactc
    12901 ctattcttcg tttaagctcg gatctatatt aatctccagt tccattgtaa atgtgtcaga
    12961 ttgaaaaaaa tagctgtttt tcctttttat aagaaaaaag gcaaaaaaaa cagaaagtag
    13021 gtcctccaag acactcttcc tgttcccaat ataataaatg aaagtaagaa attatatttt
    13081 caagaaaaca aacagaaaat tatcataaca caaattattt ataattgaaa gactagtttt
    13141 ttttcaagaa aagcatacaa tagattgtgt tagtaaatac agaacacact acatttagtg
    13201 tcttaaggag tcctcataca tgaagtcata ttttcagttt ttagctcatc agctaaaagg
    13261 ctggccgcaa caaaattatt atcttttctt tttcagtctt ggctgccagg tcatgactct
    13321 tgtcaatagt cccatcactt ggctgtccgt tattacattt atcggtacca cgcttggtgt
    13381 attgtgtatt ttatccatca atgcggcaaa atcagtcaac ggtattttag gtattttgtc
    13441 tgcgatttgt tttattatcg ttgggttttc agcaaagaac tatttaagta tcggtgaaca
    13501 gattgcttat atgatcacat tggatatccc cgtattgtta agtaaagaat ggaatcataa
    13561 tatggcttca aaaatccgaa aattcactgg aaaaagctgg tttatcgcaa tcatcagtac
    13621 tttccttgtc tatcttgttt caggattcct gattggtcgt tatacagatg atcctcggcc
    13681 gtgggtagat gccattgctt tttctattag cttgactgct ggtatcatca gtttcttgcg
    13741 ttacaacaac caatattact ggtggttagc ttcagggctt gctcaaatgg tcctttggta
    13801 tatttcattt acgcatggat ctgcaagttt ggcgatgttc gtgaacagct ctgtttactt
    13861 gctgaatgat gtccttgcat ttaccgtttc accttggtat aaccaaaaag aacgtgaacg
    13921 tatgcaaaaa atggaagaag aatactacaa cgcacaacaa cacgcataaa aaaactgagc
    13981 gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaat
    14041 cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt
    14101 gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctatt ttttatagta
    14161 attccttcag atactttctt gcctcatcat tgaaacgcag tgattcttct aacttctgat
    14221 aatattccaa cgcttgtttc tgagcttcag taaaacgata atgttttcct ttccatttgt
    14281 cgattgtctc ttgcacattt ccagattgga tatctttcac tactagttgc catttttctt
    14341 ttgagactgt acggtctttc ttatcagcag attgtatagc ttctcgttct ttacgcaata
    14401 cttttaaaaa accatttctg actatccgag gaatgctgtc gaagaaccct tctgtaaaac
    14461 catagcgaca atattgcttc caaataaata gatagatttt tggttggatg aatcgttggt
    14521 ttttcgcaaa atattccatc tgagcagtga cttctggtgt tcgttgacgt tttgtctttc
    14581 tcaacacgga aataaatgtt tcaaaataag cattaggatg tactggcact tcttcatata
    14641 cgatggattg ccaatcttta cgtaaaaatg cgcgatagtt gatcaagaat tcttctacat
    14701 cctttactaa ttcttctgca aagtcgtcaa atagttcttt ttttatctgt cgcttttttg
    14761 actgcccatt gatgtgttca cttattttct tgatttcatt ttccaatgct tgataatccg
    14821 ctatgtaaat cggcatttct acttggcgaa attctgcgag ccgctgagct aattttatat
    14881 ccggtaaata tggctgtttc atccgacgat tttggtacaa gacaaaacag tatttttgcc
    14941 atagtccttc aggaaaatcg atattcttct gcttaagcca taataattcg tctaaggcca
    15001 gatcaacttt tgtcattccc tgatggatcg aagctgccac tgtcggagag acatgcaggt
    15061 gatcggcaaa gtggttgatg cctagtttta ggatctttcc ggtttttggc gatttaacga
    15121 tgtattgcac ttttaattct ctaccacact cacaatacaa tttttgctgg cttcgttttg
    15181 ttcgaaaaaa cggattgatc ttgaagtcag aaaacaccca atctgttgca gctaaataat
    15241 gttcttccat aaaacgagag ctgattagat atcggcggtg cttatcgact agttcccgct
    15301 gttttttcgt taattttgca tagatctccc aacgttgact gcttgtgtat tgtttagaaa
    15361 ttttgatggg atagcttttg ctgatttctt ttggaaacca ttcttcaaag acggattgcc
    15421 attcttccac ggctgtttct ttcctttcta attacaaaaa gctggctact aacataacgt
    15481 tagcaaccag ccagtgtttc ttgctaaatt agtgagttgt tccggcattt ccggaaatat
    15541 cttctttatt ttctactgct gcttcaactg cataagtcaa agaagaagca atgtcttcta
    15601 aagacataga aggcgtaccc actggacgat cgatcacttg atcgggtgag aatggcacgt
    15661 ggatgaaccc acctcgaatt gccgggaatt gtgtttcgat caaatgaagc aaacgataca
    15721 tgatatcatt gcagacaaat gttcctgctg tataagagat gtacgctggt agaccatgat
    15781 cttgtacatt ttttgtcatt gctttgattg gcaatgaggt aaagtaagcg gctggaccgt
    15841 tttcttctaa agccgtatcg aatggctgat ttccttcatt atcagggatt cttgcttctg
    15901 ctagattgat tgcgacacgt tcgaacgaga ctgctgaccg cccacctgct tgtccgacac
    15961 agatcacgac atctggttgg tgctgttgga tggcttcttt gactacttga ctgctttttt
    16021 cgaatacggt cggtacttcc actttgatga tttcagcacc gctgatttca tctggcattt
    16081 tttttactgc ttcataggct ggattcactt tgtccccacc aaatggatca aaacctgtta
    16141 ctagtacttt catctgttgt tcctccgtcc gattgattta acatatggtt atcttttatc
    16201 ataccatttt ccttgaacag cgtaaataaa ggaaacaata gtttaaataa cataacagat
    16261 caaacttaat atattcttat aaaaaaatag tgttccacta caatgttaga agaggttgcg
    16321 acaattattc tgtcacaacc cctctcttct attatttaaa tagaatcata taagcaattt
    16381 ggaaaactag cataaataac gcaataaaca actggttttt gatcactccg tatttgtctt
    16441 tcatctccag catcgctaca ggcacgatat tgaagttagc agccatcggt gtcagcaggg
    16501 tcccacaaaa accgcaagtc aatgcaacca ttcctattaa cgctggattc gctccatgac
    16561 taaatacgaa tggtgcaccg atcccaaccg tcatcacggt gatggccgca aatgcattcc
    16621 ccatgattac cgtaaatatc accatgccaa gagcgtaaat gatgatgccg atgttcacat
    16681 ttccttctgg aacgatcgta cctactgcat tggaaatcac tgtcccaact ccagcgcttg
    16741 taaataccgc acctagagaa gctaaaagca ttggcaacat gcttaacggc cctactgtgc
    16801 ccagcatatc tgctgcatca tctaaaaatg tacttggtcg attatctcga gaaaagaaca
    16861 tcaagattag catggctgca aaaacaccga cgcccacgcc gactaaagca cctaaattgg
    16921 taaatagagc aaacaagatc gcaaagacac cgatactcaa agctggaata aaaattttca
    16981 ttcctagttt gtctgacatt ttttccatat attcttttga tggaagccgg ctttctcctt
    17041 tgctgacttt cttagcaatt gctggtatgg tcatcgcaaa gatcaagcca ccgttcacga
    17101 ttgccggaag ccatcttcct cctgcaataa ctacccctaa gaccgtccag aaaaaggctg
    17161 acccataacg atgaggattg gttttatctg ttaaatttct tactccagtg taaatcaata
    17221 cgagtcccat cacgatataa aggatctcca gtaatttatc gcctaataaa atatcaggat
    17281 ttgtaaaaaa gtccatctta ttgccccttt cctggataat atttcttcgc taatttcttt
    17341 tctttaagat agaagtagac acttgccaca actaaagccg tgatagccac tggaacttcc
    17401 actaaagcaa gctgtcccaa tgttacgtca tatcctagat ctttcagtgt tgattggact
    17461 agcaacgccc ctgctcctcc gatgaatagt acttggccaa agaaccagca gacattttcc
    17521 atagctgaag ctataccttt tagttcttct tcatgcttag gatggacttc taaccctttt
    17581 gattcgatgg tccccaatgc catcggtaaa atgattggac gaacaaatcc agcaactcca
    17641 ccaaaactca cgttaaaagc cgcaaagatc attcgaaaga tcccataaat atctacaatc
    17701 ataccagcag atactttctt gaatcgcttg atcaaggaag ccgctgattc tttcagacca
    17761 tttctttcaa gcgttcctgt tgccaacata atgatgataa aaatcgccat cccgcgattg
    17821 tttacaaaac ttgtacctaa aagttctaac attccttgaa tgccaagacc gccagtcaaa
    17881 gctgtaacaa ctaaagcaac cattacgatc aatatcgagt ctaatttaaa tgcaaacccg
    17941 agaataacaa tcaagacgcc taaaaggcta agataatttt ccatatattt tcctccataa
    18001 aatgacaata tttttttaat tataaagcaa aacacataaa aaatatagtt gttttctcac
    18061 gaaatagaca ctatttttaa atatttttcg ttattttttt atcacaaaaa ttatcttact
    18121 tattgtaagg taaagctttt tttaatttat tgcccgataa tatatagaaa taaggtctag
    18181 ttactgtttt ttttaaatga ttaataaata acaacttctt tctatgaaat tgcaaagcat
    18241 tcatcttctt ttccgctaac gatatatccg tcagtgcttg gtacacttta ctaaaaagta
    18301 tgagattgcc gatatctgga taggtgtgct atactacaga aaaagatata caatctgcca
    18361 ccgggtagga taaagaggat gatcattcca ttaggtcttt gaagggatga ttttcctctt
    18421 tttgtgtatt tttttatagt taaggaggaa tgtaagtatg gcatggatgg aattaatcat
    18481 agcaggaatt ttggaagtat tctggtcaac gatgatgaaa tggagtgatg gtttttctaa
    18541 aatcaattat tctttttaca cagtgattgg catgattgct agtttttact ttttgtcaaa
    18601 ggcaatcaaa agtttgccga tgagtctctc ttatcctatc tggacaggga ttggcgcagt
    18661 tggttcggtc ataattggtg tggtcttgtt tcatgataag ctgaatcttt ccacttggtt
    18721 ctttgtgggg atgctactca tcagtatcat tggcatcaag attactagcg gccactagac
    18781 acttggaata agtactgatc agaaaaaata agccttttta tttaatattc aattttttta
    18841 tgaaaaaata agaaataagt ccaatgacct agcgacttct ccactcatca ggtgtatgct
    18901 tgtactatct atagaaaagg agtgaatatt tttgaaaagt ctttcggtcc aagaattatc
    18961 caagaacttt ggtcacaaac aagccctcag cgatgtcagt tttagcttat cccctggcaa
    19021 aatcgttgga ttagtaggtc ctaatggcgc tggaaaaaca acgatcatga aagctatttt
    19081 aggactgatc cattactcag atggaacaat ctgtattggt gaaaatgaga tttctccctc
    19141 tagtcataaa gggttggaac aagtaggagc attgatcgaa tacccgggta tctatccatt
    19201 cttaacaggc tatgatcatc tgaagttatt ctctgaaacc aacgatgtat ctgctatcga
    19261 cactatcgtg aatcagctca agatggaaaa gtacattcac aaaaaagcga aatcctactc
    19321 gttgggaatg aaacaaaaat tgggaattgc tcttgcctta ctgaaccatc cgtctatcgt
    19381 tattttagat gagccgatga acggtttgga tccacaagca acaagagatg tgcgaaaaat
    19441 cattaccgac ttggctactc agggcacctc tttcttgatc tccagtcact tactcagcga
    19501 gcttgaaaaa atggtcgatg agctgatcct gatcaatcaa ggaaagatca tcaaacaatg
    19561 tacaatggct gaattgaacc aaacagcgca agatttcatc gttatctcta caacaaatga
    19621 tctagctgct cgaacgatct tgacagaagc tggatatact ttggaagatc ctgataaaat
    19681 caagctttta aaagaaaacg aagaattatt aggaaccgtg atccagctat tgtctgcaca
    19741 aaatatccat gtgacagacg tccagcatct gacgaatgat ttggaatctt ctctattgga
    19801 aattctggat tcagtaaagg gggaagaaga aaaatgacat tgcttgtaaa acaagaatta
    19861 tttaaattga tcaagaaaaa gtcaactgct gttttatctg ttctattagt tgttttgctc
    19921 ataggaacag cacttctagc caaaaaatac acgacgatca ttgatccagt tgaaatgact
    19981 gctcagcttt ttagtgccac atcttggatt gtctttatca tgattgcagc tgcaagcacg
    20041 atcatttcga tggaagcaca atatggtaca ttgaaaaatc tcctttatcg aaagtattct
    20101 cgaggagaaa tattagtaag caaatggatc acattagtta tttattctgt ttatttatac
    20161 cttttagcta ttattgtcac tgtgttgatg aaactgattc ttttcccttc gatttccttc
    20221 acggagaagg tttctactgg tcaaacactg atccaaagct tgttcactta tactttgggt
    20281 agttatatcg gtttatggct gattttaagt ttagtgttga tgattgcttg ctttatcaat
    20341 agttccggcg catccatctc agcaggaatc gtcttttatt ttgcttcgtc aatcatatct
    20401 ggtatcctta ttgcactcat ccaaaaatgg gagtggatca aatggaatcc gatcagtatg
    20461 ttgaatctgc aaaaccaaat tggaaacgaa gagatcatga agcaattgac tcatctttct
    20521 actaatcaaa tgctattcgg taatttagct tacatcgttt tgttcttagc attaggatat
    20581 ctcgtattta agcgaaaaaa tgtatgatga ctcatcaaaa aagaggctgt gaaagagacg
    20641 ttcagcctta ttgccgattg gctgtctctt aaacaccgtt tattctgaaa aaggagctgt
    20701 gacaacattt gtgtcacagc tcctttccta gtatgttttt gaataaacga caggctcaca
    20761 acctctcttt atacttcttt tatacttcta tacgtgaagc atcccagtgg caaataaaac
    20821 cattgcaatc actccgacca tcgtcacacc tataagtaaa atcttatcta agtggatatg
    20881 ttgtacaggc tcgttttgct ccttacgtgc tttgaggtaa aaataaatgc ctggcaagaa
    20941 aagaacaaga acaagcatta cttcattaaa ggctacgact agcatagccc atacttgaaa
    21001 ggcacttgct aaaagcccta ctaaaaattg gacccattct ttccgctgat aagaaagttt
    21061 catttggtac aatcctaaga aaagccacgt gaagaaaata gctgctgtac caagtgtgta
    21121 agcaaagtta taagcactgt cagtgaataa taatgaaagt aaaaagacat tcgtacataa
    21181 cgtcgtcaat acgatagcat aagttggtgc cccatttttg ttgagttttc cccatgcttt
    21241 aggcaaaagt ccgtcttcag ccatgtttcc cattgtttca atcggtaaga tcgtccatga
    21301 gagccaaaca ccaactaaag aaatgatcaa accgccgttg atcacgactg ctccccattt
    21361 accaaccatc atttcaaaca catagcccat agcaggctgc gccttgatcg tttgcagctg
    21421 attcatcgtc attgtcccat atggaagcat ggaaaccaag acgtaaataa taatcaagcc
    21481 gaatatccct agaatcgttg ctttcccaac atctgattgt ttttttgctc gtgaacctac
    21541 gacagaggct ccttcaatac caacaaatac ccaaagcatc accatgatgc aactcaatac
    21601 ttgactggaa aacgggatcg gctgtccatt cgcattcaat ataatgtcgc tagaaaagtt
    21661 atcaatgaat tgttggatcg aaaacgtgct gatagctact acaataaata aaaataatgg
    21721 cactaattta cagaatgtga cgatagcatt gacgaaacta gcattttcga ctccccgatt
    21781 cactagccat gcatatcccc ataaaaagac tgtactcatc acgatcgatg ccagattttg
    21841 cccaccttca aatactggga agaaatatcc taatgcgctc atcaacaatg ttgcaaaagc
    21901 tacgttgcct aaccagcatg tgagccagta accccaaccg ctgataaaac cagcgaaacg
    21961 gccaaaacct tcttctgcgt aactaaagat cccgcctttt aaatctggac gttttttatt
    22021 gatattattg atcgacaagg agagcatcaa cattgctgtt cctacgagta cccaactgaa
    22081 aatagctggg ccgccagccg atccggagat gtccgtcata ataccaaata cgccacctcc
    22141 aatcgctgaa ccaattacta aggcagcaag tggaattaaa ccaattcctt ttttctgttg
    22201 ctctgtatcc atgtaagcat cctccaaaat aaaaccgctt ttatcaaaaa gtataacaaa
    22261 ccttttttat ttttacttaa ttaataaaaa ctttaataga aatataattc taccggcttt
    22321 tttctcatta tacaagcaaa gcgcctagtc cctttccaaa ttacaccacc aaaaaactta
    22381 aaaaagatgc tatactaaaa ggaaagaatt ggaggatgat ccatgaacat tttatatata
    22441 tcgatttcag gaaatacgcg tgcctttgcc aaacatctag cagaatatgc tgaaaaaatg
    22501 catgcagaag atcctgtcaa tcctgaagtt actttaaaag aaatccatga aaatagtgat
    22561 tttacgaaag aaacagaacc attttttact ttcgttccta cttatttaga cggcggaaat
    22621 ggtttagata acggtgatac agaaatcttg accgaaacga tgcgtgaata tctggaacat
    22681 gaagacaatc ataaactttg tcttggtgta gtcggtagcg gaaataaaaa cttcaataat
    22741 cagtattgtc taacagccaa acaatatgca caaacatttg gctttccttt cttggcagat
    22801 tatgaattac ggggaacacc atctgatgtg acacgaattt atcagatttt agcagaaaac
    22861 aataaataat aaaaaagaag aagctgaaaa atagatttca gtttcttctt tcgtttctat
    22921 tgtttctatc atattaagct ttgacatcca tcacaggtgc ttttgctttt gctttacctg
    22981 ttttttccaa gacaaagttt tttacacgat ccatattggt taccaagacg atcatggatg
    23041 tttctttgcc tgctgctttg atcgcttcta agtcagcttc tgctacaagc gtatcaggtg
    23101 tcacttgctg cccttctgtt actttcacat caaatggcgt accgcctaag tcaactgtgt
    23161 tgattcccat atgaagcaag atttctaaac catttggcgt tgtgatccca atagcgtgtt
    23221 tcgttgggaa gacagagctg actaccccag taactggaga atagatatgt ccgttttctg
    23281 gttctacaaa gtagccttca cccatcatct tttgtgcaaa aactggatca cttgctttat
    23341 ctaaagaaac aactgttccg tcagcaactg atgagaggat agtcacactt tcagctgaag
    23401 tttgaggtac tactttttca ggcacttcat ttactgtggc tgtttcagtc gctaattctt
    23461 ctttttctaa acggtcttta ggaataccga agaagaatgt agcaacgaag ccaccagcat
    23521 aagctgccaa taatccaagg atgtagccta accatttacc gtcagagatc aatgggatca
    23581 aagcggctcc tgaaggtcca atagcgatag ccccgacatt tccgatcatt ccgatcactg
    23641 ccccgccgat acctccaccg atacaagcag taataaatgg gcgtcctaga ggtaatgtta
    23701 ctccgtaaat caatggttcg ccgattccta ggatcccgac tggtaaagct cctttgatca
    23761 tttctgttaa ttctttatct tttttgcagc gaatccataa tgccaatgct gcacccactt
    23821 gtcctgcacc agccatcgca agaattggaa gcaatagcgt acgaccagtt tcatcaatca
    23881 tttgcacatg gattggagtc aaaatttgat gcagtccgaa cataaccatc ggcaagaata
    23941 atgtgcctag aacgaagccg gcaaatgctc caccaacgcc aagtacccag ttgatcccac
    24001 cgaccatgcc atcagaaatg aaacctgcta aaggcatgat gaagaaaatt tcaatcagac
    24061 cgatggcaat caatgaaaga gttggtgtaa cgatgatatc taccgcatca ggtactactt
    24121 tatgcagttt tttctcaacg attgacaata gccaaacagc gaagatgaca cctaggatcc
    24181 caccttgtcc agctgcaaga gcttcaccag taaataagtt tttcagtggt gcttccggat
    24241 tcatccctgt cagcatcaca acggccccga taactccacc aagagttggt gttgcaccaa
    24301 aaacttgtgc agcattgata cctgtataga taactaggta gctaaacatc ccgtttttca
    24361 agatgttcat cacatcgata tattgttgcc atgttgctgc atcaagtgtt ccggctgtga
    24421 ctaagtttgc taaaactgcg gcaatccctg ccacgatccc agtaccgaca aatgcaggaa
    24481 tcattggcac aaagatatta gaaatgtctt ttaataccga tttgatttta gatggttttt
    24541 gtttcgattt ttgtgccgct ttcatttctt gtgcccgttc gtttacacgg tcttttccag
    24601 atgctgatga atttgctgta gctgtactta cagaagaaag tttttctcct aatttcacgc
    24661 ctgccaaatc aaccatttgc tgtgctactt tgttcacttt cccaggtccg atgatgactt
    24721 gaagctgttc atcttctact acaccaagca ctcctggaat cgcttttaat tcagagacat
    24781 ttacaagatc atcattgatg atcttcatac gcacacgagt catgcagtgg acaatgcttt
    24841 ctacattgcc catgccgcct gcccctttat aaatttgttt tgccagttct tccaaagtca
    24901 gttcgttttt tgccatggtc ttttccccct cataaggtcc cttttacaaa tccgtttgct
    24961 tcagctaatt ttgcttgagc ttcctcttta tcactatttg tcaaaatcat gacgatagcc
    25021 agcttaacat tttgatctgc ttcttcaaag tacttttcag ctgtttcgta atcagcttct
    25081 gtcgcttgca tgatgattcg ttttgagcgt tcgattagtt tttcatttgt tggtttgaca
    25141 tcgaccatca agttattgaa tactttacct ataccaatca tactgatagt agaaatcata
    25201 ttcaagataa gtttttgtgc tgttcctgac ttcaatcgag tagagcctgt caaaaattct
    25261 ggacctgcat cgacttcgat tgggaaatcc gcgtgtttac tgatttcggc ttgtttattg
    25321 caagaaatcg ttcctgtgac agcaccgatc gattttgcgt aatctaatcc accgatcaca
    25381 tatggtgtac gaccgctagc tgcgatacca ataactatat cattttttgt caaattcagc
    25441 ttgattagat cttcttgccc tagctcataa gaatcttcgg ccccttcgac agctacagtc
    25501 attgcttctt ctccaccagc aattagacct atgaccattt ccggttctac accgaatgtc
    25561 ggtacgcatt cagcagcatc taacacacct agtcttccac ttgttccggc tcccatataa
    25621 atcagacgtc cacctttttt aaaggactca atcgtgttag caatcactgg ttcgatactt
    25681 gtcagttctt tttcaaccgc agcggccact ttctgatctt ctttgttcat tagttgaacg
    25741 gcttgagaaa tactcatctc atctaatcca aaagtttctt ggtttctgcg ttccgttgtt
    25801 aggttctcta aattgatttt gctcattttt atcttccttc ttttcttata aattgatagt
    25861 tcataccagc tttgatttgg ttgatcaatg gtagctcttc tttgataact ttagctacta
    25921 cattcacttt ttcatccgct ggcaaatcat atttggttaa ttggatctca cccatatagc
    25981 gcaaatactt ttcgttatct aaagtaatag accctttcat acgcgtagct gtatttcttg
    26041 ctgggatagg cggaatctct cgaaaacgtg catctacgct tcgtatgaca tctcttgcat
    26101 cgtcttgccg attcgtatgc ttccctaata tatattcgta aaattcttca tctacaggtt
    26161 ccgtatggag caagactgtc tgttcttttt ggaaagaagc aaattgctcc tgaacgtttt
    26221 ctgacagacc tgcatctccg atgtaaacaa tatccgtgtt acttgctaat agatctagag
    26281 cgcctgcaag aggatggatc cctcgatgtt cttccaaagt tggcaatcct tgatatagtg
    26341 ggcctctcaa ctctgtatct cctggtacaa aaccttggac aaggagacct tgaattttga
    26401 gccattgatt ttttttctga taccattctt tgtccagccc tgtttcggga cgtggataat
    26461 aattatgcca agcttcaatc tgtgagaaat cagcttctgc ttctttcaat tcatcgatat
    26521 ctttaggcgt gatcgtgcta gcattcaaac tgattttcaa ttgatgtgac cagtcggcaa
    26581 tctgctgatt ggaaatatga taatccattc ttaaacctgt tacaccaatt gctttcaaag
    26641 gcgttagatc ttctaatgaa aaacccgctc gttccaatgc ctctcctgaa atatcgacca
    26701 tcaattctag tttattcttt ttcgcttcgc ttcctagttc gatcaatcgc ttttgatatg
    26761 ccgaaacatc atcttctggt atatgcatgg aagtgaaaat accgacaaag ccattggatg
    26821 ccatttggcg gatatactgt ttcttctctt ctgacaaaga atcgttcata aatattgaaa
    26881 agccaaacat attatccctc tttttctgta tccgtcttca tttctatgac tagagtatat
    26941 atctaaagaa acaaaatttc aatactttta ttaaaataat taaatttcat ttctaaaggt
    27001 tgagtttata ttatcaaaag aattgtcata acctaaaaaa ggcactgcta agcaaaagca
    27061 gtgccttgaa atattttatt taaataacac aaaaacataa ttactcataa gaaaccaatt
    27121 ctgtattatc taccatgaat gtaaagaacc cctcattagg catcggatga aaataagcat
    27181 tgatcactct tatcaaggca ttatggcaaa ctaacagata gacattttct tcgtcttcga
    27241 tgcattcttt caataaagga acgatccgtg catacacatc taagactgac tccccatttg
    27301 ggaatcgtac agcgaacgct aaacgtgctt tttgaaagtt ctcatctttg cttggcagtc
    27361 catcataatc cccaaaatcc atttctacca atcgttcatc aacggtcagc ggcaagctga
    27421 gacgatcagc aactgcttgt gctgtatctc ttgctcgttg aagaggggaa tggatgattt
    27481 tcgtgattgg aacaggcaaa tctactacct tttctgctag tttctgtgcc tgcatctgtc
    27541 ctttttcagt cagcggcaca tctgcccgtc cgcaaactaa tccttgtgcg ttccaagttg
    27601 tttctccgtg tctcgttaca taaagcatgt gattctctcc ttcgttggca aagttcccct
    27661 taaccatacc ttgttgttct tgagattaca agggttagat tctctcaaga gaaataatct
    27721 cagtagatac tttatccagg aaggtttgat catgttccac tagtaacatg gcaggtttca
    27781 cgttcaagat cagttgttct aattgttctt gattgaagac atccaaataa ttcaatggtt
    27841 catcccatgt atatagttca gctggctgtg ataaagattt agccaattcc acttttttcc
    27901 gttggcccat actcatctgc tcgatcttgt tatgaaaaac atctctttcc atcccaagct
    27961 ttcggaggtt gttcaaaaat gcttggtagt ctacttggtt tttctctgca aattccgcca
    28021 acgttcctcg attgtcttca taattttggc ttgcataact aatgctcaga tgttttggct
    28081 ggtatttttc tcctataacc ttgccgttga atgcccccag aagataatgg ataatggatg
    28141 actttcctgc accgttagga cctgaaatcg ccacccgctg atgaggctcg attgtaaaat
    28201 gaattggctc gaataacaga ttttcatacc ctaattgaag atcttctacg cttaaaagtc
    28261 gcttatggtg agacgcttgg ctattcatcg tcaaagaatc gatatattcg atatctttta
    28321 atagtttttc tttctcgctg atctgggtct ccatccgatt cacgattgct ttggatcgtt
    28381 tcatcgtccg tgcagcatca gcacccactg ctcctttatt cactcgtcta gattcagtat
    28441 cgatgaatcc gacttgtttc tttgttttat ctccttctcg ggaacgagac cattcggctt
    28501 tttcagctgc tgttttcttt agcctactga cttctttttt caatttttca ttttgagcca
    28561 tttcaaattc atcacgaagt tttttctgtt cttcatagat agagaaattc ccttgataaa
    28621 gttccagttg acttttttcg attgctaaaa catggtccac tacttcatcg ataaatcccc
    28681 ggtcatggct gaccacgatg aagccttgtt ttttcttttt caaataagcc gctacttgtt
    28741 ttctaccaga gatatccaaa tgattcgttg gttcatcgat taacgggaaa tgagtgtcat
    28801 ccacaaataa aagtgccagt aggacttttg tcttctctcc ccccgatagt gtgctgaatt
    28861 ctctccataa gatttcagga tctgtttgca tcaattggag ctctctttcg atttcccata
    28921 tctcaaaatc cgtaatatca tttaacacgt aataggttaa acgttcttta tcttttgtct
    28981 gttgcgggaa ataggcaaat tcttgctgat ggattacttg cccttgataa ggtagtttgt
    29041 tttgtagaat attcagtaaa gttgtctttc ctcgaccgtt tcgtccgata agtcctagtt
    29101 tccattgtgt gtcaaaattt agatttgctt gttcaaataa taatgtgcct tggctgtcgt
    29161 agccgaatgt cagatttttt atttcgattt tagacatttt ccatcacctc gtaaaaaaag
    29221 accagtccta ttttcagaac tggctaaaga ggatgcaagg gttggataca cacaaaaaga
    29281 cccatacatc gaaaaaacca agccaatccc gaaaatctgc acactgcttc cttgtctcca
    29341 caaggtacag tctgaatgca gctttcatca atcgacttaa tttttcaatg cacggatccc
    29401 tcactttgtc ctatatttga aattacgata gcataaagaa aatgattttg tcaactactt
    29461 cgggaggatg gtgaaaaatc tgctaagtgt ctgtcctaag atttttgttt tttctaatgt
    29521 ttgcctgtgt tccattgctt cttctaaacc tattggaccc aaatgaatac ttaagacaat
    29581 tgggagaaaa tcagaaagtc cttctagctc ttggtctcta cttccgcaaa tagcgattat
    29641 tggttttcga tatttttttg caagcactgc tacaccgtat ggaacttttc cgtggatcgt
    29701 ttgctgatcg atccgccctt ctcctgtaat gatgacatct gcttgttcga tctttttttc
    29761 caatccagtg atttggctga ctaaatcgaa accagaaacc atcttcgctc ccaacaaaaa
    29821 aagggctcca ccaataccgc ctgctgctcc acttcctgga atatcatcta aatccacctc
    29881 ccagaaatct tttatttgct gagcaatctt tcctgcttgt ttatctaaga aagctagcgt
    29941 cgtatctatt cctccttttt gtcttccaaa ttgccaagca gctccttgtt gacctgtata
    30001 tggattggta acatctgcag cgataatcaa ttttacttta gaaacaagaa cgtttcttcg
    30061 atcaaaatga agttttttag tagataataa aggatttcct tctgtgtacc cttctaaaga
    30121 agcacccaag gattggagta atcctagacc accatcgcta cacccgcttc ctcctaaagt
    30181 gacaataatt tgttctactt cttgaagcag tgcatcttta ataagttctc ctaaggcata
    30241 actggaagca agtcggatag tttgatccgt tggtttatca agtagatcta accccaatgc
    30301 ttgtgcagat tcgatgactg cggtgttttt tccttgccaa gttgtcaata aatagctgat
    30361 ttttcgctga cggaataaga gatccatccc atcaatggta cgccatgttc ccctgagcgc
    30421 gtaatagatt gcttctgcag tattttctcc gccatctgca atcggcacat tgatttttct
    30481 tcctttacga tcttcccagc catttagaac tgcctgattt agttcaaatg aagaagcact
    30541 tcctttaaat gaatcaattg ccgccaaaat atccatctgt atccctcact ttcttcttaa
    30601 gtaagacact ttttttcatt tttttcaaac aaaacaatgc tattttcttc catttttcta
    30661 tatactaaaa gggaactcat tgaataaagg agagaaaaaa catgaaaatc gcaatagctg
    30721 gggccggagc aatgggcagc cgaatcggat tgatgctaca tcaaagcgga aatgaggtcc
    30781 tcttgatcga tcgttggcct gctcacattg aagccattcg tacaaatggg ttgattgctg
    30841 actttaacgg taaagaagtg gtcgcaaaac tgccaatcta ttcaccagaa gaaatcatcg
    30901 aaagcaacga acacgtggac ttgatcgtag cattaacaaa agccaatcaa ttagatgata
    30961 tgttttgttc gatccaatcc attattactg acaatactta tgttttgtgt ttactaaatg
    31021 gattaggtca tgaagatgta ctggaaaaat atgtgccaaa gaaaaatatc ttatttggga
    31081 tcacgatgtg gacagctggt ctagctggtc ccggaaaagt cacattgtta ggtgatggag
    31141 aaatcgaact agagaacttg gaaccagaag gcgaagcatt tacaaaaaaa gttgtagaag
    31201 tctttcaaga agcaaattta aatccgatct atagccataa cgttcgttat tccatttggc
    31261 gaaaagcttg tgtaaatgga acattgaatg gtttatgcac gattttggat tgtaatattg
    31321 ctgaactagg cgcgcaaaaa gcggcagaga gcatggttcg aacaatcgta tctgaatttg
    31381 cttctatcgc agcaaaagaa ggaatcatac tagatcaaga agaagtgtac caacatattg
    31441 cttctactta tgatcctgac aacatcgggt tgcattaccc atctatgtac caagacctga
    31501 tcaaaaacca tcgtttgact gaaatcgact acatcaatgg tgccatttgg cgaaaaggac
    31561 aaaaatatga tattgctaca ccatattgtg cctttttgac ccaattagtc catgcaaaag
    31621 aaggaatcct tggcgcagaa taaaaggctc tctgatgaga atcagctgaa tcagcagctg
    31681 gttctctttt tttaagccca ataaaagatc aaaaagaaaa cacaactctc atgatgccat
    31741 aagaattgtg cttgtttgtt tcagtacata atctcttcaa aatgtaggtt ttttatttgt
    31801 tttcaacaga ggagaggtca tattcgtatt gctcataaat agtggaatcg ttagtgataa
    31861 atcgttttgc ccaattttct gcatcataaa attccaatag ctctgatagg attggcttaa
    31921 atggtgtatg cagaaattcc ggtaattcat ctaaatcacg ttctcgaagc aaaaaaatat
    31981 tttcaggacg ataaaaatga taatgagcaa tcgcttgatt atccgtcact agtttctttt
    32041 gaaaatacat ggattcaaat gtccgcaaag tcactccttg ctgaccctcc cgcaacagtt
    32101 ccagaatccc gcgcgattcc cctgtttttt tcaggtactc ttcataagta agatatccag
    32161 gttgttcatt ccctcgatgt ggaagaatga aatagttggt cgtaacatct ctttttataa
    32221 attcctgctt atagctttct gctcgttctt ttctcccatt attcgttgca ccaaaaaata
    32281 gatccgtctt tggttcgcta gaagaaaaat tcatatgttt cgaataaaat gaactattat
    32341 gtttcagccc aagttctttt gcttccaaaa aatcaaaatg ataaatctca tcgattgccg
    32401 gttcatcttg tgcctgcttc agcatcgcct cgtgttctgc tttgaagtga ttccaaaaaa
    32461 ataacacaat ttttgcattg gtaaatgggc gcacgcgtcg agccatctcc atatcaaatc
    32521 cataatctaa aaaaacgatc agctgataat ttgccaactg ttgtctagct gatttaatga
    32581 attccgactg tagtaaatct tttccggacc aaacatctaa agagcggatg gaaggcaaaa
    32641 aataaagtga tttatttttt gcaactagca gtacgtcatt tttgttcatc ttttccgcct
    32701 cactttctct gtcacttttt cctatgatag cctgtttaaa tagtaaaaag ccagtaaaac
    32761 gtttcctata tttgtatgaa aatttaacat ttgtatgaaa atatgatgaa ataaatcatt
    32821 cttctattaa aattcgccta aaagttttct gaatagttgt aattgtctga aaatttgtta
    32881 tcattagtca ttgttaaata ttgctattta ttcttaatat aaattttgat tgtgaattta
    32941 aaagaaagag gtcatttatg gaagataaaa aattgaaaaa acaacttagc tccagacata
    33001 tcaccatgtt agctttaggt ggagcaatcg gtgcaggtct atttaaagga agtggcgaag
    33061 cgattgggat cgccggccct tctgtattga ttgctttttt gattggtggg ctgattttat
    33121 atatcgtgat gaaaggtctg ggaaaaatcg ttttaagcgg tggcgacacg catcacggat
    33181 tatctggttt gatacgccct tatctaggtg ctcattctgc tgattttact gattgggtct
    33241 actggtctat gtggattatc aatatcatcg ctgaagctgt tgcagctgct tcctttttgc
    33301 agttgtggtt cccgcacatt ccagcctggg tatttgtttt tatgttggct gttttaacta
    33361 cgatcatcaa tctttattcg gtacggttat tcgcagaaac agaatattgg ctggcttttg
    33421 ctaaaatcag tgtcattatc ttactgatca tttttgctac ttatcttgtc ggtcagcaaa
    33481 tgttgggcac aggcgttttc cctacccttc agcaattgac tgaccatggt gggtttactc
    33541 ctcatggtat gaagggaatc atcagttctt tgcttgtagt tatctattct tacggcggtt
    33601 ctgaactgat tgctatcacg gtaagcgaag cagatgatcc taaacgtgcg attcctaaag
    33661 caatcaaagg tgtcatggga agaatcattt ccttttacat cattcctttg tttttgcttt
    33721 tgattctttt tccttggaat actttagcag gcacgaatgt cagtcctttt gtgatggtat
    33781 ttgaaaaaat gaatatccca tttgcagcgg atatcgtcaa ttttgtgatt gtccttgctt
    33841 tgttctcatc tatcaattcg ggtgtgtatg catcttcaag aattttgttc ttccgtttga
    33901 aggatcgaaa aggttcctct agaaaactag ctgttttaaa caagcatcaa gtaccgcaac
    33961 gtgctgtttt attttgtacc agtgttcttt atcttggtgt agctctttct tattttgtcg
    34021 gtgacaaatt atttggctat ctggcaggtt ccctttcgta tacagtatta ttgatctgga
    34081 tcattatcag tgcggcatcc tttgtactgg cactaagaaa aggaacatta tgggataaag
    34141 gaatcagctt ttttgcatta gctgtcttag gattgatttt ccttggtatc ctgttcacga
    34201 actccattgg ggtaactgta ttgactggcc tgctttacct gttcatttat ttcagttatc
    34261 aaaaaaagaa tgatgcgttc gtattgacga atgaatgaaa tcgatagaaa attttatttt
    34321 atttatgtgg aatagatgga gctattgaag aaaggagttg tgacaaaagt cttgttgcaa
    34381 ctcctttttc cgaataaaca gtctcatgac ctcttggtaa acggtgttca aaaaccctct
    34441 gtgcaaaata attgattgtt ctccatgctc atgacatgat agaagaaagg aatgaaaaaa
    34501 aggagtttgt tttaaatgaa aaagtattgg aagtttttgt tgatgatcgg tgtttcaacg
    34561 gtagttatgt ttggtatgat gtacttgaat gtctatgcgc tcgatcatat cttcttcagt
    34621 cgtacacgag tattcatggc gttgatgatg ggtgcagtca tggcagtcat catgctgttg
    34681 ttcatgtgga aaatgtatga aaacaaaaag ataaatgtta tgatcttagc cgctagtctt
    34741 cttgtttttg ccggttcctt gtttatggta cgaagccaaa ctactgtcaa cgacacagct
    34801 tggatgaagg ccatgattcc tcatcattct atcgctatcc tggcaagtaa acgagctgat
    34861 ttaaaagatc ctgaagtaaa aaaattagcg gaagaaatca ttcaggcaca agaagaagag
    34921 atcgacaaaa tgaaaaaaat gatcaaagaa atggaatgac cattatccgt ccaaataaaa
    34981 aaagaggatg ttccagaagt tatcagcttc aagaaataag aagaagctac ttctggaacg
    35041 ccgtgaataa ggggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    35101 aaatagcttc tcctattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa
    35161 caccgtttat taagaggttg taaaatcagc gttctgacct gagtattaga gagacaaatg
    35221 gaaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga
    35281 acaccgttta tcgagaggtt gtgaaaaagt cgcttagttt cgcgtattaa gaaaaatttt
    35341 gtgaaatggc tttccacatt tttgtataaa tttggcttaa taccgagaaa ctggcttttg
    35401 aataccgttt attcgtaaaa gggattgtga caaaagtctt gtcacaatcc ctttttatgt
    35461 ttttattgtt ctaattgcgc tgtcttgctt actgcttctt gcggtttcac aaattttttt
    35521 gagattcccc aacctgtgaa ggcactgacc atcgaaaaca caggagaaag caaactgaaa
    35581 aatacgaatg gcaagtaagt gagtgtagga ataccaaatg tacttgctac gaaacttccg
    35641 gcaattcccc aaggcactaa gtagttgatt actgttccac catcttctag cgttctacct
    35701 aatacagttg gatcaaatcc tgctttttca taagtttctt taaatgcatt accaggcaga
    35761 ataacagata ggaattgttc accaataaaa atatttactc cgatacttgt catcaatgtt
    35821 gtaaaaatca aatttggtgt gttcgtcatc tttcttgata ctcgttccat cacggttgag
    35881 atcaagccaa attcgatcaa caatccacct aatgacaaag ttagaataat caatgacaca
    35941 gtaggcatca tactttcgat tcctccgcgg ctaagcagtg aatcgatttg ttggtttcca
    36001 gtatttgaaa caaaccctga ttcgatgacc ccagccaact tcgttaagga agtttgtggg
    36061 ttttgaataa aaatcattcc tactgctaca gcgatattca acaaaatcgt taaaattgcc
    36121 ggcacctttt tccatgcaca cagtaacatt aaaaataaag gaatcagtga ccagattgaa
    36181 atagtaaagt ttgcttctag aacatcagtc acttcacgaa tagatgaaag acttgcggct
    36241 ttacttgtgt gtcctaaaat catgaataaa aagaaagaaa caacaaatgc cgggacagtt
    36301 gaccacatca tatgcttgat gtgtttgaac aaatcggcat caacaatagc tgctgctaga
    36361 tttgttgatt cggataatgg agacatctta tctccaaaaa cggcacctga aacaatagct
    36421 ccaacaacca gcgctggatt taagcctagt gttgtcccaa taccgaaaaa tgcaatccca
    36481 atcgttgaca taacagtaaa ggcactccct acagcgctac ctacgattgc acacacaaca
    36541 aatacagaag ggacaaacca ctggacactg atcaatttga acccaatgac cattagagtt
    36601 ggaatgatcc ctgcttgtat ccatacagca atcaatgaac cgactaaaat aaaaataaag
    36661 attgggatga ttcctggttt gatgccattg atgattcctt cgtgcagcca atctgtcggg
    36721 tatcttttta tcactgcata caacatgacc attccgatcg ctgctaagat cgtgatatta
    36781 ggtgagacct ttaatccgac aacgccaatc gcaatgccta aaacaataac cattaataca
    36841 agtgttgctt ctaaaaaact tggtgttcct ttttttccca tgataatctc tcctttttta
    36901 gttgttctta tgttctaaaa aagttaagat atcggattac tccgccgcaa ttcatccgcg
    36961 attcctcctt tataaaaata aaaagacctg ttttaaacta aggacgaatg actccgcggt
    37021 accaccttaa ttaaaaacag atctgttttt cactttcaaa cttttattca aatgtatgga
    37081 ctttccatgt aattcgtcaa ttaggcttga tcggttcaca gcgaccaccg actttctgaa
    37141 caaaacacct aaccgctact gcgtgaaaag ttgacttgta cgaagtttac cttccataag
    37201 atcatttgtc aatgaaaaag aaaatatttt ttcatttttc tgaaaattat ttttataact
    37261 ttcacacaca agtttatttt ttactagaaa gtagcgccct cttgtttttt tcttgctact
    37321 atttcttgat gttcttgctt gattctttct aatagctttg gctctttcaa cagatcaaga
    37381 gctgtattgg cgagtagttc tgctgcaatc gcaatagaag caaggccttt gtcactttta
    37441 gcagcagctt tgaattcttc tgtgtgtccg gcaatatatg tctcagagat tgctaccgta
    37501 ggctggattg ttggtatgac ttggctgaca tttcctacat cagaagaacc gagacttgtt
    37561 ctttgttccg tttctatttc ttcatctgga acacctgctt cttttgcatg ctggaagaat
    37621 aagtcatcaa aagaaggagt aacgatgaca tcatcaactg cattttggaa caatccaaac
    37681 tcaaaagtcg tgcccgtact tatcgcagca cctcgtacga tattttccac tttttcatat
    37741 actttatcta atgtttgccg atttcctgca cgtaaataga aacgtccagc agcgtaatct
    37801 ggaatgacgt tggcagctac tcctccgtct gtaatgatcc catggatatt gacatctttt
    37861 ggcaattgca agcgtagagc gttgattcca ttaaacactt ggatcaatgc ttccaaagca
    37921 tttactcctt tttcaggtgc tgctgctgca tgggaagcga caccgtaaaa tttgatgtcg
    37981 actggatcat tcgctaaaga ttctgtcgtt ttcccataac gaaaagctgg atggacgcat
    38041 aacgctacat cgacatcctc aaaaaaacct tctcgaacaa agctgccttt agctgatccg
    38101 ttctcgcccc cttcttcccc tggggtgcca tatacacgga tttctccacc agtttcatca
    38161 atgaattgct tcactatgct ggcggctaat acggagtaag taccaaacaa attatgcccg
    38221 catgcatgac caatacctgg taaagcatcg aattctgcca aaaaggcaat tgtgggaccg
    38281 ggtttattgg aaatataacg tgcatcaaag gctgttcggt gtccagctac ttctttttct
    38341 acccaaaacc tttctttttc taattgttta atcaacgctg ctgaggaata aaactcatag
    38401 ttacttactt caggatgttc gtggatatct agagcaagtg cttgatattc tggtaaacga
    38461 tattggatta gatccttcaa ttggtttgtt gagattgttg ttgtcataaa aaattcctcc
    38521 tatgctagtc atttttctta ttttctcccg aatgtttccc atgcaggcac gcttgatcct
    38581 tttgatgttt tctcgattac tttgactgtg tcttctgttt gataagcgtc tactattttt
    38641 tgatacgttt tattgttttc atcttctttt cttgcaacga tgatattcac ataaggacgt
    38701 gcatggtcat ccactggttc taagaagatg gcatcagaag caggattaaa tccagcatct
    38761 acagctacac cgctattgat cactgaaatg tctacatctg acaaagcacg tgctgtttgg
    38821 gaagcatcta attcagaaat gtctagattt tttctattct cggtaatatc agaaacagtg
    38881 ggcgcttgtc cttttgcctt atccactttg atcaatccag cagtttgcaa taacagaaga
    38941 gctctgccgc catttgtaac atcattaggg attgctactt ttgctccgtc tttgatatca
    39001 gctacatcct tcaaagtgtt agaataaatt cccagcggtg cattgacagt atttccgatc
    39061 gatactaaat cagttccgtg ttctttattg taattgtcta aaaagaattg atgttggaaa
    39121 gaattcaaat caatcgatcc atctgctaaa gccacatttg gttgattgta atctgtaaat
    39181 ttcacgtatt ccaaattaat gttctccttt ttcaaacggt ctttcacgtt gtcccataca
    39241 tctgtatctt ctccaataat ccctagtttt acgatagttt cttttttgtc agttgtatct
    39301 gcttttcctt ggctacaccc tgccaaccaa ataattgcta caaatgtaat gactacaaaa
    39361 cgaataattt ttttcatttt ttctcctcca ctttcaatgt gtaatttttt taatcagttg
    39421 attactgata aattgactta aaaaaactaa gattagaatc aataatgtag ccacaatcgt
    39481 tacatctgtt tggaatcgat tataccctct cgatatagca agattcccta atccgcctgc
    39541 acctactgct ccagccatgg tagtcagtcc gatcacgttg atgatagtca acgccgagac
    39601 ccgaatgata ccaggcaaac cttctaacaa atacacacgt aaaatgatac cgattggact
    39661 ggttcccatc gcttctgccg cttcgatcac cccaggatca acttctagca gtgcgttctc
    39721 gatttgacga gcaaaaaacg gaacaactcc aaaaatcaat ggcactaaag ctgctttcgc
    39781 accaatcgtc gttccaacta agaaacgagt agtcaatgct aaaagagaaa gcaagataat
    39841 gaaagggatg gaacgaatga tattgatcag cttatcaagt atttcaaata gcagacggtt
    39901 agctagtaat ccattagggc gtgtcactac taacgtcaca cctaagatga ttccaatcat
    39961 cccgccaatg aatgctgtcc aaaaagacat atatagtgtt tgcttcgtcg cttcgacaaa
    40021 ttcatccgga atcaaataaa cattgggcaa atatgtttta aagaaatcag tcattttatc
    40081 ttcctccttc gatcactttg atacgactat ctaaatcgtt tttttgtaaa atatttattt
    40141 ttacaccaga tttgatcaaa taatccaatg cttttttccg ctgtttggtt tctccagcta
    40201 aagtaacgat caaactccct aacggcgttt cttgtaataa ctctacattg ccatataaaa
    40261 tattcgctgt tacttgatat ttgctgtaca gatgagcgat tagaggttcg ttcgtctgat
    40321 ttccaatata acttaattcc accagccatt tattggcgat ctgatcggca aaagcagaat
    40381 gggcaatgat cgtttctaat gcttggtcaa gatgagtagc tgttcgaata aaatctttgg
    40441 tcaattcttc ttcaggattg ctgaagatct ggatgctgct acctttttca acgattcttc
    40501 catcttccat aaccgctacc ttgttgcaga tttctttgac aacttgcatc tcatgagtga
    40561 tcaggacgat tgttaaacct aactggcgat tcaatttttt taacaatgca aggatctgaa
    40621 gggtcgtttt cggatcaagt gcactggttg cttcatcaca taataatacc ttaggttcat
    40681 tcgccaaagc tcgtgcgatt gctactcgtt gtttttgtcc ccccgacagt tgtttaggat
    40741 aagcagatgc tttttcttcc aaaccgacaa gttccagtag ttcattcact ttttgacgac
    40801 gttcctgttt cgattttcca gaatacttta aagaaaaatc aacgttgtca aaaatagttc
    40861 ggctttccat tagattgaaa tgctgaaaga tcataccgat ggcctttctt tcttttcgca
    40921 attctttggg agagagctga ctcagattcg tctgattgac aatgacttct cctgctgttg
    40981 gtttctgcag caaattcatc acacgaacta acgtactttt ccctgcacct gagtaaccga
    41041 cgattccata aacatcgcct ttttcaatcg ttaaatctac agaatctact gcctgtatct
    41101 ctttttcttt ttgtttgaaa catacagaaa catctcgcaa ttcaatcatt aaaattcctc
    41161 cttatctaaa aaaagcgtcc tcaagaaaat gaaaatgtca ttttcttgag gacgcttttt
    41221 tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca actcctcttc aagtacgatc
    41281 atactctagc aagatatcgg tcgcagccgt catcgcctta catctatgct cgggatgaat
    41341 tgctcgatgg ccattttcca atagtcgttc cttgcttctt ctcagctacc gaagctctct
    41401 gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa taatattcaa aaaaaaagtc
    41461 cttttactaa cataaactat gtcagtaaaa ggacgacgta tcgcggtacc accttttttc
    41521 ggaagtttcc ttccctcaag cccaacatct caaaaagaga gattggcgtt cgttttaacg
    41581 gtcgaacagt tccgaatttg cctacttatc gacaaattat tctccaaggc cattttcagc
    41641 ttttcttact tgactcttct caccagccag agcctctctg caaagtttcc aaacttactt
    41701 tccttttcaa cgaaatcgat attttgttgt tgggattaat ctacatgagg aaaactgaaa
    41761 agtcaagtac gaaattcaaa ttattttttc tatatttatt tttctgtttt tttatttaac
    41821 atttcaataa cagttcgaag atcttttcct tccgcaagaa gtttcaattt ttccttttct
    41881 tcgacggtat aatttcggac aaaactggaa tcaggtatca aaacaacgcc taatcctgct
    41941 cgtgaagcag ctgttgctcc tactaaagaa tcttccactg caaaggctga actagcttct
    42001 aatcctgttc gttccaaagc tgtcagataa ggaatcggtg aaggcttgtt ctccgacact
    42061 tcgtctccga atgtaaacgt gtccaagtaa tcagctaatt taaagtgatc caagatatct
    42121 gttgctcttt tcgtaacagt ggtcgtagca atgcctagtt tcatatgacg ttcttttaag
    42181 tctgccaata tttcttttgc atatggtttt aagtggatgg ctccgcttgt cagctgttga
    42241 taaaaatatt cttcacgctt cgctcggact tcctgtactt tttcagggct acctgctgct
    42301 ttcactaaaa tatcaaaact ttgtttccag ttttttccgc cccaagcgtc aacgatttct
    42361 tttgggatat ggatatcaaa ttgctgaaat ccccaaagcc agccatcacg atacgttttt
    42421 tctgtgtcaa ctaacaaacc atctaaatca aatattgcac ctttcatcta tacattaatc
    42481 ctttccaaaa caatacacat agtttagcac gactggtcca aaaagaacag aagcaaagaa
    42541 ccagccttct cgtttataag agttcttgga atcgacggat atagattttc ttcacagctg
    42601 tcacaagcgc cagataagcg atgactgtag caaataacca taaccagtaa ttgccaggaa
    42661 gtggcatcaa gcctaattca gcaccaaaac ttgtaaaagg aaggacagaa ccgacaataa
    42721 ttcctaatgt agtgataaca aacatggcaa atgttgcgtt gctttggata aatggtactt
    42781 tcggtgtacg cagtgcatgg agcactaagg tttgtgtcca tagtgattcg acaaaccatc
    42841 ctgcatggaa taatgcgatg aaaatcactc gctgtgaact atccaatgta tggaaacttc
    42901 cgcccactac agcaggacaa ataacaaaat acatcaacaa gtaagtagta atatcaaata
    42961 tcgaacttgt tggaccaaag taaaccatga attccccaat actagatgca ttccattttt
    43021 ttggctcgtc caaatattct ttatccatcc gatcccatgg caaggaaatg caggaaacat
    43081 catagatcag attcaaaaac aaaagttgta aaggcagcat tggtaaaaat ggcaggaacg
    43141 tacttgcaat cagcacggaa aacatattgc caaaattaga acttgctgta gccttgatat
    43201 atttcatgat attcccaaaa gtttctcgtc cagaaagaag gcctctttcc aaaatcgtca
    43261 aatctttttc taaaaggatc acgtctgcag attctttcgc aatatccaca gctgtatcta
    43321 cagaaatacc gatatcagcc actttcattg caggagcgtc attgatacca tctcctaaaa
    43381 atccgacagt atgaccattt ttacgtaaag ctgtcgtcaa acgtgctttt tgctgaggat
    43441 tcaattttac gaatacttca tggtcttcag ctacttgtgc taattcatga tcactcattt
    43501 ccgagatttt ctcaccggta accaattcat tgatctctag acctacttga cggcacactg
    43561 aacgtgtaac taattcgttg tccccagtca gaacttttac agctacacca tgttctttca
    43621 gagctttcaa cgcttgtttc gttgtttctt ttggtggatc aaggaaagca agataaccaa
    43681 tcaaaaccat gtctgactca tctttgactg aaaattctcc aacgacgctt ggattggtct
    43741 tttgtgcgac accaatcact cgtaagccat cttcattaag tgactgtact tttgcaagta
    43801 cggcttcttt tcgttctttt gttaatggtg taacagtacc gccaagatca ataaatttag
    43861 aaatgttcag catttcttca atcgcgcctt ttgtgatcat ttgtgtttta ccagctggat
    43921 cagcaaccac aacactcata cgacgacgtt caaaatcaaa aggaatttca tccacttttt
    43981 gataattgat cttatctgta tctaaagttt ctgttgcttc atcaatgata gccacatcta
    44041 acaaattttt cagccctgtt tgatagtagc tatttagata cgcatggcgc aaaacacgat
    44101 catcttcatt tccttcacaa tccatatgat attcaagaat gattttatct tgtgtcaatg
    44161 tccccgtttt atccgtacat aacacatcga ttgcaccaaa gttctggatg gcattcaaat
    44221 ttttgatcac tgttcctttt ttggccatca cacttgctcc tttgaccaaa ttcgtcgtaa
    44281 cgatcattgg aagcatttca ggtgttagac caactgctac agatagtcca aaaagaaaag
    44341 cttctagcca atcccctttt gtaagcccat tgatgacgat aactgctgga gccatcaatg
    44401 acataaattt gatcagtagc atggatgttt tatggatgcc taattcaaag cttgattgaa
    44461 ttggtttttc agatagcgtt tctgctacat gaccaaatag tgtttcattt ccaacagaaa
    44521 caatgattcc ttccgcactt ccactgatga cattacttcc cataaagaca agattttcgt
    44581 aacttgtttc gctttgctga tcgtcttcac atactttcgc atgcttttct actggatagc
    44641 tttcccctgt caatgctgct tgggaaatga ataaatcctt tgcggacaat aaacgcatat
    44701 ccgctggtat catatcccct gctgaaagcc gaacaagatc tccacagacg atttcttcca
    44761 taggaatctc tgtgtacttt ccattacgtt ttacagctgc cgttactttc acaagtgatt
    44821 tcagtttttc agccgcttga ttggaacgta cggactgaat caacgtcatt gtcccgctga
    44881 cgataaccat agtgaagata atggctacac cgaaaagatc tttatctcca ggagctgcta
    44941 atacatattc cgtgatgaat gaaataattc ctaatgcgat caacactaat gtaaatggcg
    45001 tgatgtatgc tttcaatatt tctacaatga atggtgtttt tttgccatat gaaatcgcat
    45061 tttcaccgta attttctctt gaacgttcga tttgttcttc tgtcaatcca tttgctgatg
    45121 catggaatag ttcaaataat tcttgtttcg tttttccagc aaaagctgca tagatttttt
    45181 gtgtatcttt ctttttttcc atctcaatct ctcctttgtg tccaaaagac agctgttctc
    45241 gctgcttttg gtctttagct attcatcgga tcgatagaga aaaaactgaa aggggctgag
    45301 aacagataga taatagccct ggcctttcct ctagatcctt gtttttcgaa tgactgtcat
    45361 ccatttgttc tcatctcctt tactaagtaa ttttaattca tgacttccaa tcatttttcg
    45421 aatagttatt tttccaatta gaccacctcc tttttatttt tcaccgatgg ggatttgcgg
    45481 caacaaaaaa acctctcgcc aaatactcag cgaaaggttt gaaaaagcat attaaaaaaa
    45541 gcttctcaga tctcatcgtt gagttttgac actgtacaac gtaaaaagca gatacctgct
    45601 tttagctacc ttatgaaaag ccttatctcg acaattcctg ttctacccat tggcgtctct
    45661 cgacgttttt gggcagtagc ctatgtttgt ataggagcct cacctaacaa atcttattcg
    45721 gttttccttc gttaatataa caactaaaaa agaggatgtc aaatcaattt tccatgtttt
    45781 tcatactttt tttaaaatac acacgttata ttcctgtctc acgcggtttt atttgtctca
    45841 ttttccacct cccgctataa tagaaatata gtaggaaacg aggtgctgtt atggaaagaa
    45901 cacttgttat tatcaagcca gacggcgtga gacgccattt agtcggaagc atcatccaac
    45961 gttttgaagc aaagggactg gccattgctg aaatgaagtt tgaaactatg actcctgaat
    46021 tagcaaaaga acattatcag cacttgactg agcgcagttt ttttgatgaa ctgatcgatt
    46081 acatgacttc cggtcctgtc gtttatcttg tgctggtcgg cgaggaagtg atcgatatcg
    46141 tccgtaagat ggttggagca acaaaagctg ccgatgctgt tccaggtacg atccgaggag
    46201 actacgcgct gccggggact gaaaacatca ttcacgcttc tgattcccgt gatgctgcgg
    46261 taaaagagat cgcccgcttt ttcccgccat tcgatataaa caaaccgtct gaaaaagcgg
    46321 cgaagtgata caaattctaa actgctggtc tagttgctgg gttcagcaat ggccagcagt
    46381 tttttctcta aggacctccg ttgattcttt cctaacatat attcgagcac tgtgaagaat
    46441 tcttggtagt ctttcgtctt ttcttcccct aaagcagcag aaatcaatct ttgataataa
    46501 tctgccatca atgtttcata gatcactgta cttctttctt tatgtgctaa gctccgtttc
    46561 atatcttcat tcacactacg tgcctttttc aaccgaccat tttctatata aaacgaagtc
    46621 aggttcatta aaaaagtaaa gagatactgc tcatgattgt tcaactgtgc taatcgtcgc
    46681 attctttttt tgattttcaa ataattgttc tcaatgaacc gatcactgaa tatccaagga
    46741 aaatcagcta gaatagatag ttcaaattga ccatagtctg gtatctgcat caagtattct
    46801 tcaatgattc ggcattcata acgtaaaaat tcaaattctg tctcagatac ttctgttttt
    46861 tcgaccatgt gaagattcaa aaccaaataa tacagatctt ctgttttctt aaaatgccta
    46921 cggttattgc tgatctcttt ataaggatca aagctgtctt cttgtacccg atctagcatt
    46981 cgtctttttt tctttaactt tattggttca tccagctggt ttcgcatcca ttcatattct
    47041 gcaagtgtca tgttcattcg ctctaaaaat gatctcaatg ttacaaatga catttttttc
    47101 actgaatttg tctcaatccc gactagagaa gtcttaaaac agattccttc cgctaattgg
    47161 gactgtgtca acttcttgtt gactcgaagt ttcttgatca attcaccatc atacataata
    47221 gttctccttt ttaatttaaa tttttccaca ttcaataaat agatacgtta aaaaggaaaa
    47281 atacacgaaa aaaataaaaa aatgtttttt tcataaaaaa taccaacctt caagcattag
    47341 aatgttgtct aactactgaa ggttggtttg attgtttatt gattattttt ttgctgttca
    47401 acgtctgttc ttgttactgg atataagttg gttgttgttt tattaccatt ttttgaaaag
    47461 acacttgtcg aatgttcgcc tttttgcttt tcgattgctt cattacgttc cgcttcattt
    47521 ttatagtcat attttttcgg atcaatcgtc tcaaatccgt ttggcacata aaaccgcaag
    47581 agattttcct gattcagctt atctgaaaga cttaacgcag tcttgacttt ttcctgatct
    47641 ttggagattt cctcatcgat tcctgctgct ttcgtatcga tgatttctcc tgtctgatta
    47701 ttgtaagctt ttcctcctag gacggtgtat ttgggtgtca caaagttccc atttcgaaaa
    47761 gcaactactt gatcatgctg agaagataat aagtctgtac caaaatgaag ataatcttta
    47821 tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat cgatttctcc accgtattca
    47881 tgattgactg tccctttagt ataaccaggc atatggatca tcaatggcac tcgttgcatc
    47941 tgtgcgttgt caaattcatc ccatgtatct ggatctttac caagagcgct tgctaaatct
    48001 ttattatctg tattcgagat cccaaaatga tccccgtaaa tcacaaagat tgaattttgg
    48061 taaataccag attttttcaa atacgtaaaa aattgttcca atgattgatc cagataatgt
    48121 gccgttcgaa cataattatc tactgtgcta ttgccagtat tcaaagatgg aaagtcgaaa
    48181 ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg aaagaaattt cgtgtaaaac
    48241 ggctgttgta gatgctcaag atattgggca gattctctga ataaatcttt atctaatatc
    48301 ccataaccta acgtttcgtc ggattcatca aaataagatc gatcaaagaa attgtcataa
    48361 cctaagtttt tatatacatg atctctattc caaaaactag ctacattccc atggaaaaca
    48421 gcgcttgtat agccttgctt ttgtccaagg atcgctggtg cggcttgaaa gacattgtct
    48481 gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa atgtgccagt ttccaacata
    48541 ttttcagcat cacttgtctt tccctgtcct acttcatgaa agaaattgtc aaagctgatc
    48601 gttgcttgat tctggaaaat actatttaaa aacggtgtga cttcttgtcc gtctaccttc
    48661 atattaatca aaaactgctg gaaactttct aaatggagaa cgatcacatt tttcccttta
    48721 gcgatcccaa atgtttcggg atttggctct gcataatgtt tctttgtata atctaaaact
    48781 tcatcaatcc cattactgct tgcatgcgcg cgaacactgc tagtcattcc tgttttgatt
    48841 ccgtcataaa tagtatacgc atcgattcct agatatttga caatatatga acggtcaaat
    48901 gttcgctgca gcaattgtgg acggttggct tcgctcagcg ctagatttcc tgaaaaaata
    48961 agccctgaaa gacaggtaac agcgatagcc atcggtttgc cgacagggtt gcttttgata
    49021 ggtattttct tgaccttcaa ccaaaccagt agcccaatga acacagcgat atccagccag
    49081 tagatgacat catggggctt catcaaagaa aaggagcttc ctgacaatcc ttgcgacact
    49141 ttcgaaaagc ctaatacaga tttgactgta atgaaatctg tgaattcccg ataaaaaata
    49201 atattcaagt acaacaaaag cgtatttaaa atatcaatga tcaaaagaat agctaacgct
    49261 ggctttggct ttttaatata caaagaaagg ccaaaaaaca ataaggtagt cgcaattggg
    49321 ttgatccaca agataaagta ctggatcgta ccttccacac ctaatgaaaa atctgcatag
    49381 taggcaatga tcgtcttcag ccagaaaaag aaaacagctg tcaaaacgat tgctaaacga
    49441 ttttggcaca attttttaag ttgtgtcacg tatatcactt ctctctattc acattttttc
    49501 ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt gttatcttaa gattttttta
    49561 ttctttctaa agattattcc gtccactttt cacagcataa aaccgctgcg atgtaagcga
    49621 ttaaactaat gaagagataa aagtcttgtg gtgaccgctt cactccacgg tattcaaaag
    49681 ccagtttctc ggtattcgta caaaaaaaga ggctgtgaca aaattcttgt cgcagctcct
    49741 tatttcttga tgcaggacag ctttttcaca acttcttcct taaacggtgt tctcctgcca
    49801 actcctcggc cacaagtcac aatatttata aaacatgaga agctgttttt tgaaattgtt
    49861 ccttgtggct cagagttaaa cggcaggctc aagacctctt gatacacggt gttcaatcag
    49921 ctgaccctcg gtattagatt atgtcaggac gctaatttta caacctcttg gtaaacggtg
    49981 ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatataa ggagctattt
    50041 tttgaaattg ctccttgtgg ctcagagtta aacggcaggc tcaagacctc ttgatacacg
    50101 gtgttcaatc agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
    50161 ttgccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct ctgtttttca
    50221 ttgtccatag gattgatatt gtccttgtga tgactcattc gtaataattg aggcttgatt
    50281 ttcttcactt cccgttatcg tttgtccact gccagaatct gaaggacttg ttcctgtcga
    50341 ctcgtttttt tgagaagaga cagttgtttt cttgacttgt tcatttgtct ttttccattc
    50401 cgctaaacga tcggcgacca cttttttgtc tactttttca gaataagcgg tgatcacttt
    50461 tccatttttc aaacgggcta ctttgtcgcc atcatgagtc aaaagcaaaa cggtcaaatg
    50521 cttgtcttta aattgatatt gtctgagaga aatcgcacta agtacgtcat cgatattaat
    50581 aaattgattt tcttcttttc cttttggcaa ataaatgtcc aattcgcctt tttcgtaatt
    50641 ggcagttatt ttatactccg cccaccaata agttgagaat tcttttttga aattatctac
    50701 tgattctttg cttgctacga ttttttctgt ttctttctga gcaagatctg ctgtcatttt
    50761 tcctgaaaat gctgcggata aaataagcag taagaaaaaa ccgaataacc aaaagagaag
    50821 aaacggtctt tttttcctca tgctttcctc cactcctatt taattttgac tattctatta
    50881 tatacaaaaa atgaaataga aagacaaaaa agttgaaaaa gatcgtaaat aattgaactt
    50941 ctttttcaca aaatggtaag aatggagaat ttcacgcatt tgctctcaac tattctttct
    51001 taagcctcaa atccgttttt atctgatacg tctttgaacc aatgacccga ttttttgatt
    51061 gttctttttc catcttgtgc taagtctact gaaataaagc catatcgatt cttatatgca
    51121 ttggtccacg accagttatc catacatgtc cacatatgat agcctaaaca attggcacct
    51181 tcttgaatgg cttgatggac atatttcaga tgagattgga caaattcgat ccgataatca
    51241 tcgtggatca tgccatcttc tttgataaat cgtgcttcgt tttctacacc cataccgttt
    51301 tcagaaataa agcagtcgat atttccataa tgatctcgta cattgatcaa aatatcatag
    51361 attccttgtt catagatctc ccatccgcga taaggattca ttttacgacc aggcatttca
    51421 taattgtcga aataatcctc aggcattgga ccgtttttag tatcgattgg tgtttctttt
    51481 gcttttaccc ggcgtggctg gtaataattg atacctaaaa gatctactgt attttctttg
    51541 ataaccgtca aatcctctgc atggatcaca ggcatgagat caagttcttt tactatctca
    51601 atcaattcac ttggaaactc gccttttaca gctggatcaa ggaatgaacg attgaagaac
    51661 gcatctgcta tttgcgctgc tttaacatct tcagggtttg attcgtctct tggatagctt
    51721 ggtgtcagat tcaagatgat accaataaca ccaccttgct ccatttcatg gtaagatttg
    51781 atggctaacg ctgaagccaa cgcttcatga tagccaactt ggatcgcttt tttcatatct
    51841 gactcttctg gataatgcca tcgatataaa taacctgctt cgactggaac gattggttca
    51901 ttatgagtaa accatttttt tactttcgaa ccaaacaatt caaaacaact tttggcatat
    51961 ttttcatagg cttcaaccgt ttctcggttc aaccatccgc ctttttcttg cagatacatc
    52021 ggcatatcga aatgatacag attcacaaag ggttcgatac cgttttcaat caactcatca
    52081 aaataacgat gatagaaacg aacggcttct tcgttgattg ttccagattc atcaggtatc
    52141 aaacggctcc attggataga tgttcgaaat gaattatgcc cagtctgttt catcaactgt
    52201 acatcctctt gatacttcgt ataaacttgc gaggtttttt caggtcctac ttgatcaaag
    52261 aacttttgcg gttctttttt gtaccaataa tcaaaaatgt tctctccttt tccatcgcct
    52321 gaaatccttc cttctgtttg tgggccactt gctgcagaac cccaccaaaa atttgctgga
    52381 aattgatatt ttttcatcaa agcacctctt tttctttttt agttcgtttt agttgattcc
    52441 atgtatgata ttttccatca caatcacttt ttcattttct tcctcttctt ttttgattcg
    52501 ttttaacagc tgttctgctg ctcgttcccc gattttatct gttggtggcg tgatcgtgat
    52561 tttcggaagg atactcagat cgtttttatc taaataatca tagtgactga acgcaatatc
    52621 cttcccgatt ctgacacctt cttgattgaa acattcgatg gcaccaatcg acatattgta
    52681 attgctgaca aaaatagcag ttgccccttt ttctaataat gcttttgccc cgtaatagcc
    52741 gctttttcta gaatagtctc ctgtaaagac ctctaattgg atcgatggat cattttggac
    52801 cgcttctttg gctcctgcta aacgttctct tgctacgtag tctgtttgag tggcagcaat
    52861 aaatccgatt tttttatgtc cttgttcacc atgatgagcg atcacttttg ctacactttc
    52921 tcgatcattc actaatattg tatcaatacc tgctggtcct tctggacaat tcaatgcgat
    52981 aactggaata ctcatttttg tgagcatttc aaatccagac cactcttctt cggataagaa
    53041 gacaatcagc ccatcgatca catgcttcat caggtattca attttttgtt caattttttc
    53101 tggctgatcg gcaaacccgc ttaataataa gctataccca ttttcttcag ctactctttc
    53161 gatccctgag acaacttccg acccaaaacg gctggaaatc ccgtttatca aaagtccaat
    53221 agagaagctt cgattattct tcaacccttt agcaatgatg ttttctttat agtccaacgc
    53281 tgaaatcgct tctgctattt ttttcatgtt cttttccttc agttgctgac cattcaagta
    53341 acgggaaact gttcccactg aaacaccagc catcttagcc acatcacgga tcgatgccat
    53401 acattctcac tcctgccagt tacatttgat ttctctttct tgttgttcga ttatgatgac
    53461 cgcattcttt gcatagtgtt gcttgtcatc aatatccata taactaaagt cttcagtaga
    53521 gcaacttcct ctcactatta attatactta aacaagtatt tgaaccggtt catttttaat
    53581 atagcatttt tttaaaagat tgcaacactt ttttaaaacg ctttctgaaa gtggcggatt
    53641 ttttgccttt ttaaaatttt attctaaaat ttactctatt tgtgggtgtt ttgtgcgttt
    53701 ttctcttcca tccgtttgaa gttttacatt ccaatggtat aatttcttta tcctatcaaa
    53761 tgggggaagt attttgaaga aattaatcgt actatttatt ttgtctgcgg gacttttgac
    53821 attgactggc tgtgatttgt ttggcggctc tgaccaatcc tcggacgaaa caaccgattc
    53881 ttctgtctta aaagcggaaa aagcagatac caccaacagt gtatatacgc taaaagtacg
    53941 taaagatatg gatttatccg cgggacagtt gacatttaca gataaaaaaa tggaatggac
    54001 acgcacgtat cagacacaag gaactgattc ttctgaatca tctgaagaaa aaacagtgct
    54061 gacagatatc aagatcacta ccaaagatga cgaatatatt atttcaggaa aagaaaataa
    54121 aaaagaaaca acagtcatat ttacaaaaat cggtaattac cgtttgaagg atgaggatgg
    54181 caacatctac agtctatagt gccgtctgtt ggatcttctt ttgtttctcc ccttcttttc
    54241 tgctatagta aagaagcaag atgattgtaa aggagacgaa atggatgatc gatagtcctg
    54301 attattgtac atgtaataac aacaaaaata tccatacaga atttaatgac caaggaagct
    54361 tgagcatatg cgacaactgt cacaaactag tcaaagattc atttaaaaca tttgaatctt
    54421 atcctgttca aaattatgta aaaagtagaa gaagataaac aaatccaagc aaaactcact
    54481 atggctgatt tttatgaact tcaaactcag cttcttcctt tgatcaagct tcatcagtaa
    54541 aacaaacagt ggatcatttc agtttacaaa tcaaagagta aagatttatt tatttcaaat
    54601 atttgaatat aaaataacca aacgatagaa aggcttattc tacatcacgt agtaattttt
    54661 ctttcccgtc gtttggtttt ttatttttag cctaattctt gaccttttgc atgctggtta
    54721 tccgtaaaat attttccagc aggctttcct tccaattcag ctaatttttc ttggatctga
    54781 cggatcacct tttttgcatc ttctggattt tctacaaaat cgaatttatc cccatccact
    54841 tgtattttag gacatacatc aaaatcttca taccattgat tatagcgggt gttcaacgtt
    54901 tcataatagg tatataacgt ctcatcgctt tctagctgtt catagtcacg cccacgtttt
    54961 ttgatacgtt ctagcatcgt gtccaaagaa actttgatat ggaccatcaa atcaggccgt
    55021 ttttttggag caacatcatc taactcgttt agcatcgtat ctaataagct atcgtactgc
    55081 tgtacttcga tatccgttac ccgacctaaa tcagcgttta gatggaacaa caggctatct
    55141 tcatagatgg atcggtctag cacattgtca tcgttcacta aagcattttt cattgctaaa
    55201 aatcgcttgt tcaaaaagaa gatttgcaat aaaaaagcgt actgctcagg atttgaataa
    55261 aagagcggca atacttcgtt atcttcgaca ttttcataaa atggtttaga atcaatctct
    55321 tttgcaagca tatccgtcaa actgctctta cctgcaccaa tcgttcctga aatcaaaatc
    55381 actgacatac aacccttcct cttctataag tactcgccta cgtaagtttc aacaggcaat
    55441 gtttcaaaat catattcacg tacagcttgg tctgatttta aatacgtatg gagactttgt
    55501 gtaatcatca agttcgtagg taccgtctct tcttgtacaa tgatcatcgg ttttttcaac
    55561 atagttgcca cacctaattc gtaagccgtt cctggatcga ttgtttgatg ctcaaagtca
    55621 aggatagcaa caacaaattc tgcatttgtc agttcttcca tatctttttc gtatacttcc
    55681 tgcgcccatc cagcgctgaa tagttcataa ttgctttctt ggtgatgacg tggagagtag
    55741 aaacttgtga ctgttttgtt ttcctccaac gctttttcga tacgacttac tcgatcgatt
    55801 tgctcctctg aaaaaaatgg tcctgctaaa tatatttgac tcattatttt gcctccttca
    55861 ataataactg atttttaata gatactgctc gtaattcatt gaccagttcc aatggattgt
    55921 tggaaaagat tacttgatcc aaccattttt tatccataaa atcctctttt gcaaaagttt
    55981 ctaataattg ttgcaagccg ctgtaataac cgccaatatt caatattccg attggcttgt
    56041 cgggcgtcag accgattgct atccaagaaa gaagttgtga gaattcctcc aatgtaccta
    56101 gacctccagg gaaaacgagg acagcatctc ccttttcaaa taataatacc tttctttcat
    56161 cgatcgtttc tgtaggaata aatgtagtaa catcttttcc aggcaattca gtttcaaaaa
    56221 gcccattcgg atagatacct gtgacttttc cgttattttc cagtactgtt ttactaatca
    56281 ctcccataat cccggagtat gaaccaccgt aaaccagttc gatttcttct tgtgccatgt
    56341 attttcctaa cgtttgcgca ataaatttgt aactttcttt attaccaaaa cgggaaccgc
    56401 aaaaaacagt aagtttcata aaaaaacact ccctaactca aaagtctatc ttcattctac
    56461 acgtgttttt ttaaaataaa agactagatt ttgtagtttt ttagtggtat ataggataga
    56521 cgccactata tatagtattt agaaaaaaat catttttcag ccattttata gataaaacaa
    56581 aaataaataa cgtgttttca tcgatatgaa atgacttact tatagaagat actgggattt
    56641 cgtcctatat gccaataacg tgtattattt taaacaacaa atacttgttt cttttattaa
    56701 cgaaaaaatc attttgttct cgtgttatgt ttcactgata aatgatgttt ctctaaataa
    56761 aaaagccgac gaactaaacg atcaaaattt gatcattttt gttcatcagc ttctcctaaa
    56821 aatctttttg ttaacgataa atagctggat ttgtaaaaat atcacttgaa tcgtcactgc
    56881 tggaagaaaa ttcagtgata accgcacctg ttgcgcctgc ctggaactag tgtttgatat
    56941 tgggctcgaa cgtgtactga tctctagcat gaagaataat ttcttttttt actgtataat
    57001 aactttcttt cctttttgga atagtacagg aaagtttttt tgtttccttt ccttcgacat
    57061 aaggtatacc agaccctttc gacaacgaaa ggttctcatt tttccttcat cgtcatttca
    57121 aggagggtgc cgatgctctg gacagtttcg atcgctccta aaccaaaatc tgtatattcg
    57181 attctttcta actctgattc tttcatacca ttcctgtctc aaggtacatc tattgtactt
    57241 ttttcttgta atccattctg cctctcctaa ctaattgtca aatcatccca agatatgtct
    57301 tcttcctctt tttcttcttg gatgatttct gcattcggat ctactggttt tttcaataat
    57361 aaaatggtta ttcctgtgac aaaagaacca gctaacaatg caccaatgaa tgcccatggt
    57421 ctggtcatcg tagcgactgc aaacattccc gatgccggaa tacggctatc tgcgccccaa
    57481 aacatgctta atccgccacc tactgtccca ccgactcccg ttgctatgac attgcgtgcc
    57541 aagtcgttca atacgatagg gaaacatccc tctgtgatct gacagatccc cataggaaaa
    57601 gcggtcttca atgtatcgat ttcttgacga ttgagtatat ttttacgaat gattttccct
    57661 aagaaataag cgatcgtcaa gccgaatggg gttaccatcg aagctaaaat caatacagtg
    57721 attggttcat agattccttc tgaaaatcgt gccacacccc cttatccaga ataaatggta
    57781 atccagaaac agccgtgtat ctgcggtaat aagcctaacc aaacgaaaaa tatgagaagc
    57841 tatttttgtt tggctgttct tattacttga tgcagggcag ctttttcaca acctcttttt
    57901 ttatttatgt ttcataatca atatggtaag gatgcagctc cgtatggtag accgcctccg
    57961 aatattcaat catttcccca atttgcgtag acgatttccg tatccgtttg atgccgatct
    58021 gtgcgtctgt tttcagaatt ccttgttctt cttctgttaa aaatactgcc gagaaatcat
    58081 cagaaaatga gtcaatagta tatccttttt tcgtcagcaa acgatacaaa gattctttgc
    58141 caaattcttc tctgctgaat tgcatcattt cttgtggaag ataatgaaca aaataaatat
    58201 aaggctgatt gttcaaataa tataagcgct taaaacaaac agcttgctca ccgaaaaagg
    58261 aaaacaaagg atcttccttc tttatatgga ccaattgcaa atccagcgtt tctttccgga
    58321 cttcatatcg ggagttctcc aagatttgcg taaagcttgc cgctttggat aatcgattgt
    58381 aaggacgatt gcttagaacc gtcgtcccac gaccactttt ctttgcgaca tattcatctg
    58441 atgccagcag ttcgattgcc tttcggacag tgattttgct gacttgaaac agttcttcta
    58501 attcactttc tgtcggcaac atcgaaccga ctggatattt tcctgagaaa atatcttctt
    58561 taattttatc tgcgacttcc aagtatagta cacgtgtcct tttcataacg tcaccctttg
    58621 ctttactatt actaaaagaa tagcacataa aaaataaaaa aagaacacaa atcacatgtg
    58681 agaagtgtgt tctttttcat tcagctgctt taattacaag tttacgccat ttgaggcaat
    58741 tacttcttta taccaatcaa aagatttttt ctttgatcga ttcagtgtcc catttccttc
    58801 atcgtctaga tccacataga taaagccgta acgtttactc atttctcctg ttgatgcact
    58861 gacaagatcg atacatcccc aaggcgtata gcccatcaaa tctacaccgt ctgctactgc
    58921 attagccatc gcttcgatat gacgacgtaa ataatcgatc cgataatcat caataatcgt
    58981 gccatcctct tccatatgat caatggctcc aagaccgttt tcaacaatga ataatggttt
    59041 ctggtaacga ttgtataatt cattcaatgc gattctcagc ccttctggat cgatctgcca
    59101 gccccattca cttgcctcaa gaaacggatt ttttactcct cccagcaagt tcccttctga
    59161 agttgctgct tgtggatcag tctcattgat tgccgtagac atataataac taaatccgat
    59221 gtagtccact ggatattctt ttagcaactg gaagtctttt tccgtttgct ctaatttttc
    59281 cggatcgact ccatattttt tatgggtacg cgctgtgtat gcagggtatt ctccacgtac
    59341 ttggacatcc gtgcagaaaa aattgaattc ttggttttgg atcattgttg ctgcctgatt
    59401 gactggattg gcatcgatgc tgtaagttgt tgcatagata atcatgcagc cgatttggat
    59461 atccgaacgc aattcgtggc cgatttttac ggctttgcta cttgcaacga attgattatg
    59521 ccatgcttgg aaaatatttt ggtactctcc tgctccattg ctttttacta atccttgact
    59581 taaagctgga aagtgaaagg ccgaattgat ttcattgaaa gtcatccaat atttgacttt
    59641 actgctgtat ctctctaata ctgtctgagc aaatcgttca tagaagtcga tcagttttcg
    59701 atttttccag ccaccgtatt cttttgccaa atgaagaggc atttcgtaat gagaaatcgt
    59761 gatcacaggt tcaatatcat attttaagca ctcatcaatc agctgatcat aaaattctaa
    59821 tcccgcttcg ttaggagtac cttcgtctcc atttggaaaa attcgtgtcc aggcaatcga
    59881 aaaacgataa catttgaatc ccatttccgc aaacaaggca agatcttctt tgaaacgatc
    59941 gtaatgatcg attcctcgat gattcgggta aatatatttt tcttgatcaa tcgtccagtc
    60001 aaattcttca ctgccgataa tcgcaaagcg ctgcttacct ccaggcatag catctgcaac
    60061 ggatagtcct tttccgtctc gatcataagc cccttctaat tgatttggca gcagttgctc
    60121 caccccataa aaatttttct gaaaaacgtg attgcatcct gatttcctcc ttattttaca
    60181 attgccagaa catcttcgtt tgcaataatc tctttttggt ctaattcaag tatatcggca
    60241 aatgcatcag tattcgtaat gacaactgga gtgattacgc tatagccagc tgctctaatt
    60301 gcttcgatgt caaaacgaat caataaatct cctgctttga cgagatcccc ttgtttgact
    60361 aagatctcaa agccattccc atttaattct actgtatcca tcccgatatg aatcaaaatc
    60421 tccgctccgt cagtcgttgt gatccctaca gcatgtccag ttggaaaaat agtcgtgatc
    60481 tctccgtctg caggtgcaac tagttctcct acttctggat caatagcgat accttttccc
    60541 atcacgccag aagaaaaaac ttggtctggt acttcactca atggcagtac tttacccgtt
    60601 aaaggacttt ttaaggtatg ccgtgctgaa gtaatcggtt ttcctgcatt cgcatgtttg
    60661 ttttctaatt gattgtcttg tgtttgttcg tcaaatccta aaatcaatgt acctacgaat
    60721 gcaagaacaa aagcaattcc tgtagctatt actgctactg tcacatttga ctctacacca
    60781 tccaccgtat taatgaaagt cggaatcgtc aataagctga ccaaactgct ggagaaagct
    60841 tttactccgg aaaatccgat aatagctcca ccaatacctc cagaaataca agcggcaatg
    60901 aatggtttct tcaaaggtaa tgtcacacca tacacagttg gttcagtgat tccaaataac
    60961 gaagtcactg tagaagaaat ccctaatgct cgaagtttcg tatcttttgt tcgcaatgct
    61021 acagctaaag cagccccacc ttgtgccaag atggctggaa gaagcattgg catcaataca
    61081 tcaaacccat attgttcgat attcaaaaac atgatcggta cgaatcccca atgcatgccg
    61141 aacatcacga atacttgcca taaacttccc atgattagac ctgctacgat tggactaaat
    61201 ccataaatcg agttgaagag accgcctaaa gcattaccgg caaccgtccc gattggtcct
    61261 aaagcaagaa atgttaatgg aaccatcact actaaaacga caagcggaac taaaatcatt
    61321 tgcagaaatt gcggaatcac tttttttacg aatggctcta atttactttg aacccatacg
    61381 gctagaatga ttggtatgac agaagaagta taaccagatg gtgaaagaat cactgggatc
    61441 ccaaagaaac tgatagcttc tcccgcacca gcaactgctg tgatagctgg atacacaagt
    61501 gccatagcaa ttgctactgc taaaaactgg ttggctttga actttttagc tgctgtaaaa
    61561 gcaagcatga caggtaagaa ggtaaagaca ccatctgctg cagcaaacaa tatttgataa
    61621 gtaccgctat cagctgtcag ccagcccatc accgttgcca atgataagaa tcctttcaat
    61681 accccggcgg ctgccatcgc ccctagaaaa ggtgtaaata tactagagat aatatcgatc
    61741 agacgattca aaatattttt ggactcttct tttccatcct ccgaaactgc aagaccttcc
    61801 tcatccacga tcgattgata aacatctgct acattactgc caatcactac ttgatactga
    61861 ccgccacttt gtacgacttg gatcacgtcc gggtcttctt ttaacttttg cgtatctgct
    61921 aaagattcat cttttaatct gaatcgcaac cgcgtagcac aatgtactaa acttttgaca
    61981 ttcttttgtc ctcctaccgc ttcccataca cgacgaccaa ctgcttgatt atccattgtt
    62041 ttttccttct ttctttttta tttaaaaaat aaaaacccag attgaaaaaa acagtcacaa
    62101 acattcgtga acctattttt tcaatccagg ttttgcctgc catacagtaa caatccttat
    62161 gattggatgc tttttttctc acttactact ctttgaatat gaatcgttaa ataaatctgc
    62221 tctgcgatcc ccatttcctg tttttgcgtt tgtcgaagat actcattgat ccgttcgaca
    62281 caatgaaacg cttttggata ctttccttga actaatgcgt agagaaattc atcttgctcg
    62341 tcagatcctt cccctttgag ataacgctgg gcaaaaaact gcagatgagt aatgatacgc
    62401 tgataattca aagattcttc atcgaacacg atgccgaaga atttactgat gatactcagt
    62461 atatctctta ccattgtggt agcggtcatc gtcacttgca tgtcttcgtt tccttgttgg
    62521 ctattcacaa aatgaaaggc aataaaaccg gcttcatcct cagaaagttc cacacccgtc
    62581 ttttgtttca cgaacaaaag agcctctttc gctgcttgat attctttcgg ataaaatttt
    62641 tttgtttcaa ataataatgg attaggcagt tggatatttt cttttgctcg tgtgatcgca
    62701 taatgaagat gatcggtcaa tgtcagataa atattaggcg aaaattcaac ttttagttta
    62761 tcttcagcca gttcgactac ttgtttgacc cattcgatct cctctgccgg aatatcagcc
    62821 atcagctgct ggaattggtt tttagcaaaa gaatctctta aaacaaattc tttttggacc
    62881 gcttctggcg aaatctcgtc cccaatcttt ttttgaaaag ctagtccttt tcccatacaa
    62941 acgacttctt ccccgaatcc atttttcgaa atgacgacat tgttattcaa aattttatca
    63001 attttcattt tgcttcgccc tctcaagaaa aataaaaaaa cctagaattc gcctactgtt
    63061 tcttagctga attctggttt tgcctgccgt acagtaacaa tccatttctt attcaaaatc
    63121 atcataccat cgaattttta tttatgcaag ccttttcata taaaaaataa aaaaactgga
    63181 acacaagtct atctagaaaa caaccaaatc agagatgcac ctctttgcgt ttctcttttt
    63241 ttcagcaaat tggtttttcc ttatcgattc atatcctttg gctgttttta atctgtaacg
    63301 acttatgtcc cagtatccta aaatgttttt gtctctagat tgaaagcaga tctcatatca
    63361 tctgtcgcat cttttatcga tgtaacactt tccagttctc cttgtacaac tagtcgcgtg
    63421 acggcgtcca tgtcgccaca agtaattagt gtgacaattt cttttccctc gacttcattc
    63481 agtaattggg tatctgtagg agcaactttt tctttaaaaa atgttttgta tgtatacaca
    63541 ttttctaaat ctgtcaaata gatcttggca cccatctcta aattttctag cggtgtaaac
    63601 agcaattcag ggttcgttgc acgatggctt gctaaggcat aatttccttt tcccatttct
    63661 tgatctggag ataatgttcc tgctccgtaa agtagtgacg tattatccaa gcctttgaaa
    63721 accggtaaat tgattgaaac tgaagggact gcgatactag caatcactgg tagatctgtt
    63781 cctctcattt gcgacctcat gaccccttct gtcgtcattg gttctaccgc atcaaagtca
    63841 aaactgacat ctttatcgtt attctttttc agatcttctt ttgttacatt cgctatagca
    63901 tatttatctc ctgtctctct gacaaaataa tccttgatct gttcattgaa aatcaaagct
    63961 agtcctacta ataacaagag gaacaagagg atattgacta gccatctttt tgcttttcct
    64021 ttttttttca ttttctctcc ctccagactc attttactgt ttttcttttg ctctttccaa
    64081 cagccgaatg acacatttct acaactctgt gtattttttt cgtacacctg cgcttttttc
    64141 tacaggatat ttcaaattat ttttgatcaa tttttcttca atgatctcat ctagatccag
    64201 gtgaaggttg tctgccatca tgtatgaata gatcaataca tctgccagtt cttctttgat
    64261 tcgttctaat tgtgtttctt ttacttcttc cggcgttttc cattgaaaaa gttctaacaa
    64321 ttcagatgct tccaaagaaa tagagatagc caaatctttt tcgttatgaa attgtcgcca
    64381 attacgttca tctctaaact ggttgacttt ttccatacta ttcatttgct tgccttcttc
    64441 caatcacttc ttacttgatt aatcatacca tttttttaca taaaaaatag atgatgcttt
    64501 taaaaaaact cctgttttct tttatttaaa ataaatgtca tagaaaatca ataatttagg
    64561 tagccaccat gtgtatgcag tagcgtgtct tcttcccttg aggctttttg tttttcgata
    64621 cgttcttctt cccaaacaga caacgctact agaatctctc ttaatgattt tccttcttcg
    64681 gtgagcgagt actctacttt aggcgggact tggttatagg cttttcgatg gatactttca
    64741 tcttgttcta actctcttaa ctgctgggtc agcatttttt ttgtgatcgt agggatttgc
    64801 ttgcttctcc acgcctaaaa taagcgatat tgctcttccc tttatctggt gtaagcaaag
    64861 cagttagcat tttgacgatc gtgggttccg cttttcactt cttatctact tagtatcatg
    64921 aagtatactt tcgtactaat actaagtagc ggaagtgtca actactttgt ttaactacat
    64981 agcaaaagaa acagaagggt gacaacggtc atcacgcttc tgtttctttt gaatatgctg
    65041 tgttgttgtc tctatctccg tgtagacttt cctcaaactt atcctttgac agtaagtcag
    65101 taggctgatt tgctctttct agatgccaga tacatttcta caaaaaaata gtgattctgt
    65161 tttgaaaatt cgatttttag ttctgcattt tcgtaaacat ccaacatttt tttcgcactg
    65221 tatagaccta acccgccatt ttttttagag gtttttcctc ttttgaccat ctccgatagc
    65281 gtcaaattcg ttggatttgc cttgttcgct atactaaaac gtgatccctc tttttcttcc
    65341 agcagattga tcgtaatcgg actctgatcg gctacacgat gatcgaatgc gttagaaata
    65401 aaaatagata aacagcgaat aaaaacaacc agatcaatcg ggattgtttt cacttcgaca
    65461 ggaataacta cttggatcgg gattttctcc gattctgctt tttctagaaa ttttaaaaag
    65521 acacccctga cagctgggga tacaatattt gttagctgtt gtatttgcgg tttttcaaaa
    65581 atagctgcag aatgatcttc taatatttct aaaaattcca acgtttcttt tttattttcc
    65641 tgaagagtaa tttttaatga aaacagtaga cttttataat catgtttgaa ttcctctaga
    65701 tgatggtaat attcttcatt tatttgatac agttgaagcg attcctgatg ttgtctttct
    65761 tttacttgga aactgatcaa cataatggaa aaaaacaaag aaaaaaaact gatgactatc
    65821 gacaaaagag atagaagcag ataattagat gagcggaagt gaacacttcc caataccaag
    65881 taaatcacgc cccagcttaa taaaatacaa atacctccaa aatagtgttc agttggataa
    65941 gtaaaaagca agttgagaaa tctcgtctgt ttgtctatac tttttactaa tagatagaaa
    66001 ataaatgcga taagtagtgc aaaaaaactt gctgttccaa gatgtctctt tcctaaataa
    66061 tccatcagat caaaaaccag aaaaaagctg agtccagtga tagcaaaaaa aagattactg
    66121 atcaaacagc tgataaacag gtttttcgta gtaaaataaa aaagaaacat caataataag
    66181 ccaactaggt aaggaaagct cactttaggg ataggaagta aaagtgtcac caccatcaaa
    66241 gctatacaac caaagctgtt ttttactagc agttgtttcg aaaagaataa atagatacct
    66301 aaatctaaaa tcaaagtaaa ggtcaaataa agtgtcactg ttgcgaccat cttattcctc
    66361 cattgttttg tttcaaaatg tacacatatt tttttaatag gagcctgcat atgaaaaagt
    66421 attttatcat agaagataac cagtttcaca gcgaatggat catcagagaa ttggtcaatc
    66481 atcatatcga gaatatcgtg cagctcactt ctatctctca aatcaatcag ttgatccagt
    66541 tgggcactat tgataatcac gatgttttct ttatcgatat cgacttgaag caatcactca
    66601 caggtattga tgtcgccgaa caaatacgta aaaaaaatgc acatagtaat attgtttttt
    66661 tcaccgctta tacgaatttt gcagtagaga tcatcaatcg gaaaatcctt ccgattggat
    66721 atatcagaaa agacaggaat ttatctgaac aaattcgtca agtgctagaa cagatcaatt
    66781 ggaaagaaca gcagatcgta aatgtcgata aaatctttat tgatcaaaaa ggtggaagtc
    66841 tcgtcctgta tcctcgggaa attttatata ttgaaagtat caaaggtttg aaaaaccaag
    66901 ttatggtcaa aacaactaca gaagataaat tagtgaatac ttctttaaaa caattgaaga
    66961 cagagttaga aaagtatgct tactttttac ttttaaaatc ttactgtatc aacttatcgc
    67021 aattgcagaa aattgattct gctcattatg tgttagaatt tttcaacggt gactccttac
    67081 tcgttggtcg aaagatcttt gaaaagacaa aggaacgttt ccatgacttc caaaaaactg
    67141 cttcgagttg atgaataaga gctgagacgt aagcatagca gatttcaggg agtgggcctt
    67201 gaatcggctc cactccctga aatcgttgat atgattttac gcttcctctt ctgtaggagc
    67261 atctgttaac gaccatgtga atgttgaaac gtagttgtct acagcataat tggatacagg
    67321 aacaaataat tccactgatt gtctcccttg ttccacattc tctcctaatg aataaatcca
    67381 tgtgccaatc ccttcatttg ctcctgcagt tactagatct actgcctgat ctgctgggat
    67441 ttcgattcct tcagcgccag ttccagaaac ggtagatggc attccactgc tattagttgc
    67501 gctatttcga atcacggcgt ttttgaattt cacctttgaa ccagttagtt tattcgtatc
    67561 agatgttgta ccaataaatt ctgttcgttt gactgataat ttccaaccgg cataatttcc
    67621 gcttttatcc gttacttgta cgaaattctg tacttgtgtt tgtgctccag tactgtccgt
    67681 tccgctttgt aatgcagcat atactgtttt tgctgtacct aacttgattg ttccaaaatc
    67741 aagatctgga gcttggtcaa tcgtcagtaa tccggatgtt ccctgatttg gttcgtcagg
    67801 tgtaaaagga gtatcaggat catttggatc aacaggagga actggaacaa cgtcttcagg
    67861 atttgcagca tctggtttgg tcacagaaat cgttccttct gtagtggctg agctcttatt
    67921 ctcagcaaaa acactttgtg tacataataa tagacttcca gctagaacgg tacttaatgc
    67981 aattgttttt ttcatttctt tttccttctt tcttttaaaa taaataatcc agcaaataag
    68041 ccaataagaa tgactccaac aagtatcagc catcgccagt caaatgtttc tggctgattt
    68101 tttaaatcaa tatccttttc attcaattgg tccgctgcat catcagttat ttcaaaatcg
    68161 gtatggagat tccagcgttt ttctttccaa gcacccttca caaccgcttg ataatgtccc
    68221 ggagctaatt tttttccttc taatgggaca agtaaatcaa aatttgtatt gggtgctaca
    68281 tctatttttt ctttttttat tgaaaggtaa ggctcttttg tttctttttt gtagatttca
    68341 accgatgtag tcaagccgaa catataagaa gcatttggat tttgaagatt cgccttaatc
    68401 acatttcttc catcgctttg atccgcataa gcttttttta atttcaaatc tggggaagac
    68461 tctttttcgc tatttcaaat caagattgct ttcacttgag aaaactgatt gacgacacca
    68521 actgtacttt tgttagaaac gttctctttt ttttcttgaa acgtgatccc tcctgcgacc
    68581 acaccagcca gctctttttc tggcgctttc agtgaaagtg tctcagtgac cgcactttga
    68641 ggaggaacag ttatttctcc cactgttttt gctaagtttt tcatatcata acttaatgtt
    68701 tcatcaaatt tactttttga ttctccatat tcgatcacac cgtttgaatt cgtcgtagcg
    68761 gagtggatca atgtctcaac tgttactgat ttatctgttc ggttaaagac cgtgtagggg
    68821 atttcctgtg ttttcccagg ctccatcctt acatttatat atgttttgtt cttatcaatc
    68881 tgattagccg gtaattcaaa atctaccgca aaattgaatt cggaggcatg gacaggtgtc
    68941 tgccaaagga tgaatccaag gagaaggatc ataagtaaag agcagttgtt tcgatgactt
    69001 ttattccata ttcgattcat caatcatcct ccttgatctt tattctgtcg gcgcatctgc
    69061 taatgtccat gttatctctg ctgagtaatt accagctatc tgattttctt tggttaagac
    69121 aagtttaaac gcccgaccgt tatttaaata ttctgaataa tctgcttgca cattcgttga
    69181 attttgtcct ctgacaattt ccagttcttc ttcattcact aattgatact cctctccttt
    69241 cttgaaatat aggacatcct gcatagaaaa tcctgcatca tttgtgaaag gctgagataa
    69301 ttttatcgtt aagatccacg gatttttcgt ttctcgctga tcataaatcc ataatttttc
    69361 ctgtatatcc atttggccaa atgcttcttg tgtcaaacca gttgctttga tctgaccaaa
    69421 atcgagtttg gaaggggcaa gaaatgagag accaccttct actacgatct tcgtagcagg
    69481 tgcaaaaggc ggggcatcat gataaacaac tggcgtatca cttggattgt gattcccaat
    69541 ttcatcgttg gtacttggct tgttctgcac acccttagca gtagcttctc ctgcttggtc
    69601 gtttaataca atttctaata catctcctgc aaaaagttga ttttctggga tagaaagaac
    69661 aaattcccct gtatcatcaa tagttgtttc tgacaaaaca gcttgatttt ttttcaatga
    69721 aaccaaggct cctgcttcac ctgtacctgt gatcgtgcga gaagctgcat tgatcaatgt
    69781 ggtacttaaa ttagcaggtt caggaggaat cacatcgact gtcgttttgg cagagtccag
    69841 ttctgtcact tcttggccac ctcttacttt tttagcagca acaacttgca cttgatcctc
    69901 cgtttttaat ctctcatcat tgggtacttg aacttcgaac aagccgcctt gttcgccttc
    69961 tccccatggg ttatatcctg ccccttcatt ttcatctgtt tgtccttgcg tgagagcagt
    70021 aatttctaca gatgatttcg tcgtatctgc gtaagtaata cgtaaagtca cttctacttc
    70081 gcctgtccaa gcatctcttg gctttgcctc gacaccttcg ggaacaacga catgcccata
    70141 gattttttta tctgcattcg ttggcactct taaggattca atcatcggga gctgattatt
    70201 ggcactgatt cgattataat ttttcattcc ggcacctgtc tgagcaaatt catttgttaa
    70261 acctgcacta gggcttgata cgaaattgcc taagtcggtc ccattgatcg ataagtctgt
    70321 catatcagaa aaataatccg ggtctttttc aaagttatcg aatgttgcgc ctgtttttcg
    70381 ccataaagcg atttctgaat gggcgatctt taaagaagaa tctccatttc cttggaatac
    70441 ccagccgcct atgccattgg tctcgcttcc aatccgtttg ttttggaaat caaaataggt
    70501 aggagattct aagtccacat tcaacacatc cccactgatg atgccggtct tatcttcccc
    70561 tgttttcccg accatgacta aataagtttt ttcacctgcc gtaaaattta aatcaatatc
    70621 tccagttgta tctacagttg ctccggaatc tgcgcgtata tcgatatttg atttttctcc
    70681 agtgactgta aaatcagctg aacctggact ttctagaaaa ccagaagcgt cataaaattg
    70741 gatcgcttga tttccttgat tgagtctgtt cacgtaagat ttgccatcac ctgtatttct
    70801 taccctcaat gagcctccat tggttactgt cagcttttgc gtaccattct caaaacgaac
    70861 tgcgctggaa cgcccactct gcttatcgat gtcgataact gatccggctc cttctacgat
    70921 caaagaaagg ccttttccta caaaacgcat cccattgttc ccgggttgat cgtcatctcc
    70981 agccattttg accgagattt gtccaccact tgatacatgg atttgactag aatctccttg
    71041 caagcgaaat ccgcccgtag ttgctgtatt gatggtgatt ttcccaccat tcgatacagt
    71101 gactttcgat cctgaacctc tcatttggat gattccgcga ttgttgtcat tggtaatatt
    71161 gatctttgta tttaccgaaa attcgcttcc cgaaccactg acttcaaaca tgacattacc
    71221 aggtagcgta tcgactgcct ctccttcatc gctaacgcca tctgcccgat cataatcaag
    71281 agagaccttt gaaccgctta agatatggat tcctggcgct gtttttccac taccagtctc
    71341 atctcctgaa tctagaaaac cgtagaattt ccctagatac ggtccataaa gttgactacc
    71401 attgatcaca ttcaattgat agcttctaca aaaataggct ttacccggag cggcattcag
    71461 tccttccttc actggttgga catccaacac accggaaaca ccatctaatg tgacagttgc
    71521 acgaggagcg taaatcagtc ctaatttgga cccatcatac aaatcaagtg tcccggtaaa
    71581 ttttatttct cccgtaaaat gatatctgct tccccaaaaa tcggtcatat aagcaatgat
    71641 aatggcattt ccatcgatat ttggtgtcaa tccacctgga tgtgcagctg ttcctttaaa
    71701 attctggaac tcgatcgact gaggtatctc attgatataa atatggtgtg tgccgaaatt
    71761 gtgagcgaat ccaccaaaat caataacgat atctcgtttg atctctaatt ccctgtctaa
    71821 agtcaaaggc tgaatcagtc ggatcttagt tactgattcg tctgcgagaa gctgcgccag
    71881 ttcttctgtt gtggcagctt cgctgtaccc atccgctgaa acagctcttg ccagtgccgc
    71941 ttcactgcta tcagacgttt gttcagctac ttctgaggtc tcacttgtct tattttcttc
    72001 ttgcctttta ttagtagaag cctcactttg agattctgta gtttcagaag taatcgattc
    72061 ttctgaaaaa gtagtcttgt caaaactttg attctctgat tgcgttgatt cgtttgtttt
    72121 ctctacatcg gcaaaaacgg atgtcaccat caagagaggg aagacaaaca aaatcgacgc
    72181 aatcagcttt tttacacatt gattcattct ccaccatcct ctctgacgtt ttgacaattt
    72241 tattttatcg ttttaatcta tttccaattg tactttcata tttaaaatat gtatttttat
    72301 ggaaaaattt ttctattagg tgtaaatttc ttttttgtac aaaaaaaagg ctgtgacaaa
    72361 agtcttgtca cagtccctta tcccgaacaa gcggtgttct cccgccaact cctcggtcac
    72421 aacctccatt ttaacaataa acctgtaaat gtgagggcaa tactttgatt gtcacaggta
    72481 atttatcccc ttcatctcca tcaacattcg tcgtcaattc ttcctcatca gctaatgaca
    72541 caactaattg ctcacaagta aggtaggcta gatttttcgt agattcatct actcctttta
    72601 ataggtctgg caatgtttta agcgtatcga tcaaagaaga atcttttaaa taaaccaaat
    72661 ggagcttacc atcatccact tttgcatttg gaagtaatgt ttcaaaaccg ccgactgagt
    72721 tagttagtcc tatcagcaag gtactgctct caatttcttc cttctttcca tcaacttcta
    72781 cgtgaaacga gtaggattgt gtggaagcta actgtttgat tcctgacatg aaataagcta
    72841 gttttccaaa tttcgtcttc ttctccgcat ctacatcatt gattgattca gggatagagc
    72901 caattgcgac gacattcata aaatagtcgt catttatttt tccaatatcc aatggcttga
    72961 ctgattcaat cgaaaaatga ttgatcgctt cctcaggttc tagcggtatc cctaatgctc
    73021 gagccaagtc attcaccgta cccaatggga aaaatccgaa atttggccga tgttcttgtt
    73081 cagcgattcc actgattcct tcgttgactg ttccatctcc gcccatgaca aacacactat
    73141 gatacccttc cgtagccgct tcgcgagtaa agttttttgc atcccctgct ttttttgtat
    73201 gcaagacgac cacttcatca aacaccgatt ccaattttgc tatcgctaat tgctcaaatt
    73261 cctttgcttg ttcaccgcct gaactggggt ttaccactaa taaaactttt ttcatcactt
    73321 tctttccctt ctgattgatt tctttaatat aacgatagca taaagaaaaa aattaggctg
    73381 tgatgaagta ctgttcatga aacattcatt tattttttga caagataaaa catccatact
    73441 atgaaactga ggaaaattaa aacaatgata taggaaataa aagaaaatga taggcattgt
    73501 atacctaata aagtaaaaat cttaggtggc agataaaaac acaaaaatag caagagaatg
    73561 taatagcgtt tcttccactt tttgttatca gaccttatag cgatcagagg aataccaaat
    73621 gcataactga aaaactcttg atgtactgtt aatatgtcac ggccttgaat aataaatgcg
    73681 acaatccccg ctataattag tgtgcctaac accaaaacgg aaaattgatg aatatatttt
    73741 atatacaaat gatccattgt acttcctgcc tttttatttg tgagaaaaac aaatccgtat
    73801 acgtgaataa cttctagaaa taaaagaaat ttgcaaaacc actccattta tctacttcta
    73861 cgtgcatttt ttgatattgt tttctttatt taagattata cagaaaaata tctagggtcg
    73921 ctggattcta agtaaaaaaa atttttaaaa taaaaagaac taaatcaagt agtaattaaa
    73981 ctactgttga tttagttcta ataaaaagat ttagatgaac agttttattg atttaaatag
    74041 ttcatatttt ttatttatac cgcttattta ctacttgctg tactagaatc tgaagtgaat
    74101 gtactcaaaa cattttcaaa gtctttgtct ttgattttta cattagcgtc tttcaatact
    74161 tcgccaatta ctttattctg gaaggtactg tcacttaatt ttgtgtctgt tgcaatttct
    74221 ttcaattctt ttttgtattt atccatgtcg ttgcctttgt tttggttttt caccatttta
    74281 accacataat attccgtagt ataagttgaa gcattggtag aagtgatcac atcagaaact
    74341 tgaccatctt tcaacttaaa ggctgcttct ttgacttctg ccggaaccgt tgtagtagtg
    74401 gaatcaaatt tcacttttcc gccatcttct tttgtcgtag tatcagttga cttgtctttg
    74461 gctaatttag agaaatcgtc tcccttatca gcagctttct tcacatcttt tgcatcatct
    74521 tcacttgcta cttggataat ttgagcttcc acttctggat ggaaagattc ccaagccgtt
    74581 tttaaatctt catcggtcaa tttgatatgt ttctttagac cttcttgaaa ggctagactt
    74641 tgtttgatac tgtctttgaa tgtttgttct gtgtaacctg cagatttcaa ttgtgaatca
    74701 aatgaatcac ccaattgttt ctttgtttga tcatattgag aatcgatttc tttggttgat
    74761 acgtcatctc catatttatc ttcaaatact tgataaacga tcaactgttg cacagcggaa
    74821 tctgctcctt gcattttttt caagctatcc attacttctt gttcgctgat tgtaccgcct
    74881 ttataactaa cggctgtttt ttcaccggca ttcgtacagc ctgacaataa gccagtgcct
    74941 agtacgatgg caagtgctaa aaatcttttt ttcatttatc aattctccta cttttcgttt
    75001 tttgcgaatt gctaaaaaca aaggtgttca agatgaaaat catcaagaac acctttacta
    75061 atctgctcga tacgtcctga atagatataa ggctacacaa tccagaaagt tcgctcagca
    75121 gaataaatgt caagtagcac atcacacctt aatatactac ttcttttgtt accgtttttc
    75181 aagtctatct tgcgttccgt atttaacgta actaattctt agaacaaatg ttatcgattt
    75241 tgtttttctt cttccgctgc tgggatattc aaatcagaca cttttatgtt cgattttcca
    75301 gacatttttt tgttgttttt attatgatat atttttatac aactaaaaat gaatatatta
    75361 aaactaccaa aaataacttt taacgctgtt tcattatatg ttttttctat tttatataaa
    75421 cttttggata cttaatacca accggttgga ttgtaccaac atgagaatgt gtcagttcta
    75481 tttcctgtcg cacttgcata ggcccatact ccttttgttg catttgctga tcgaatagag
    75541 tttccaactt tcgcttttgc accgtgacga ttatagacac gactatagtg gttagaatga
    75601 cctgtttttc tcgtacaagt tgtatcacta taataaaccc atcttccaga gctatgatta
    75661 attgctgttc tagaaatttc ccggtctact ggtaaatttg acttaataat attaccttta
    75721 gcatcaaaat gaatctctcc tggctttaaa gatttgtaat agatttcacc gtgaactgaa
    75781 tttgcttcca cttcagatga catagcgttt cctccaacaa aacctatccc tagtgcacaa
    75841 ataataactg cttttttcat aaaacgacat cctttccttt ttgattttta aagcatgatc
    75901 ttcttgtttc ctattttggt cagatatgga tcatgcgtga ctacgataac taacgtacct
    75961 tgggaagctt gttgcgataa gatttcaact actttatcct tatttctttt atccaatgac
    76021 ccagtcggtt catcggctaa aatgattttg ctattcttca ataagactct cgcacaggcg
    76081 attctttgct tctcaccacc agataatgtg tagataggcg tacttagctt cttgtttata
    76141 tttacttttt tcaatgcctc atactttgct gttcgcttat ctgaaacatt taaattcgaa
    76201 aacttcaatc caatatctaa attaaaatcg attgtttcat attcaatcaa cccaaaatct
    76261 tgaaaaagaa aagatatggt atttcttttt atcaagcgct tctctctccc agttaattgt
    76321 ttcgtctctt gatcgaacaa atagaattgt cctgaactga actgatcatt caatcctaat
    76381 atattcaata acgtactttt acctgctcca ctttctccaa caacagtcac aagatcccca
    76441 gcatccgcag tgaaacgatc caaatgaaga atcgttttgt ttcgaatgat ttttttcgca
    76501 ccttttatca ctatcaacgg ctccaattgt taacctccat tcatcagcaa taactgactt
    76561 ttcttttctg tagacaaaat aaaaacaact gaaagaataa aatcaatgaa gctaaagaaa
    76621 aaaatattaa aaagatattt aggctctctt tctataagaa aaaggaagat tacacttgtc
    76681 gttccccatt tgatgaggtt catcatgaag aaatctttat acgtttcaaa gagcgaataa
    76741 ccacttgaac gaaagatagc gattttccga caattgtaga caaaatatat gcataacatg
    76801 acatagctca acaagaagaa agaaaagaaa gtgagtaaaa atatctgtat caagtaatga
    76861 aatgatctgc ttaacacttt gacagctatc tcttcagtct gagtatacgg aaatgataac
    76921 tgcaagttat cacgtaactg actttcttca atcagctcaa ataacctttc tttcgacaaa
    76981 gagcctatct tgatctttaa cggtggatag atttctccgc tgagaatatt tctttccaaa
    77041 tacatactgt ttttcaatgt cagtatttca acaaccggat aatgttcgat ccaggggata
    77101 gaaggaataa atgtataaat gggttgattg ttttttataa atagaaattt cggttctttt
    77161 tcactgattt cttgataata ttctgttatt tgcgccaggt cattttttaa atctgtatgt
    77221 ctatatcgtt cagggatcaa tataacaggg tgcgtttctt tttcggttat ttggatcaac
    77281 cttccagaga catcaatcaa aggaaatttc ttaagataat tcagatttat ttttatccct
    77341 ctgccaaact gttcattttc ttcattcaag taatctgata cattgactaa tatacttccg
    77401 tttttattca aagtttcata taattcctga tctgctgctg atgcaaaacg attggaataa
    77461 ataaaatcca cgtggttttt cccgatggtt actggataaa atacatgata gtcctcacca
    77521 gcgatttctt ttggtggttc atgggaaaca gctaactgtg aagaagaaaa aacgatagtt
    77581 gctaattcga cattggcaac ggtcaaaaga agagaaaaca acaatccaaa tgtaaaaaga
    77641 atgtattgga tatagttgaa cgcttctctt aacgaagcga ttttttcaac taataaaaga
    77701 gaaatcaaag agataccata tattccgctg aacaagaaaa cgtacatctg gaacaaataa
    77761 agaaattgct ccttataata cataaaaata aaaccaccaa tgattcctgc aattatgaat
    77821 gtaataatga ttttcttttt ccccaataaa caccacaata cttgatgtaa tgaaaagtca
    77881 ttattcaaca ataaactgat ttctttattc atcaataaga aaaaggatga taacaaaagg
    77941 aataaaagaa tcaatccaat agttaaatac actcgataat tatatacgtc tgtatttgaa
    78001 ataaccgaat acgaatagct attttcaaag tcttcaaaaa tattagtggc ataatcagtg
    78061 tgaaatgctt gattgaatcg atcagtgaat agtgaaatga accgttcata ggcttctttt
    78121 tctctcgtca tcaagaaaaa ttcaccttca aaatctttta ttttgatcga ttcttcaatg
    78181 gacgttacgc gaaaggaggt attagaatag ggtaccttct cgtgagaaga tttgacatcc
    78241 ccttgataat acaatgtcat ttcaagcttt ggctgtatct tgaagttcca agatgtatct
    78301 acttcattgg attcatttac tgtttttttc atataagaaa gctcagtttc tgatgagaca
    78361 ttttcaaata ccatgattag ctgctcatat tcttcttgcc caataaaatg ttgtggcaat
    78421 cgaatcggag tataagtatc tgtttgatgg aaaaattcat ttgtccgata gagtacctgt
    78481 ttatttatac taaccaaggc aaaaccactc atgataccgg tcactatgat tgtgagtaaa
    78541 actgtgaact tttttttgat tatcaccact ctcccttact taaatgattc tttgataaaa
    78601 tcataccgtt tttcatcaaa aaaacgtatc tatcaagtgt ataggtcatg tagaataaat
    78661 ctatatactg tagaatcgaa aggagaagaa gatgaacacc ggagaaaaaa tacgaactta
    78721 ccgcagaaat tgtaagctta cgcaaaaaga attaggcgaa aaaattggtg tttccgataa
    78781 aacgatttct agttgggaaa attctcgtac aatgccagac cttgaaatgc tgtcactttt
    78841 acatcatact ttaggtctgc cgattgattt tccttctatt gaatcagcta ccgaatcaat
    78901 agaaccaaca ttgccagatg aacctcttgt taacaagaaa tcttattcat caaaacaacc
    78961 cttctcactc aaaaagacac tatctttctt tctgctcgtc ttttgtttgg tctatctttt
    79021 tcttatcaat tatcaataca aaaatgaaac gatcgatcga ttcaatcctt ttttgaaacc
    79081 acaaatcggc tataccacac tccctgtagg atacgtggac aattccatcg aacgttgggt
    79141 atgtaatgat atctttggcc atggtacatg gctgtctttt cacgctggag aggtagaaaa
    79201 agagaaaaac catgctgtaa tcaaacacaa aggaaaacac gtcatgaacg ttcgcctgct
    79261 cacttgggaa gccattccct cagattacca agaaatcctc agccaagact atgacgacgc
    79321 cttaaaaaga agccctcagg agccagatag gcattaagta ggagatgaac gtagagatag
    79381 aaaaaatcaa tgcacatgat cttatccaaa aaacaggggt acaaattaat tgaaaaagcc
    79441 ttgagatgaa agttcctaaa taaaaaagtt cagaatgata ggtatttctg gcttatagta
    79501 taatatttac gccacagcct cccaatgatt ctttttggaa tcataatcaa ggatggacat
    79561 tcacaaaaat attttgctca ttcgatccga atagattaac gaccaatcta gtaagtaata
    79621 tagattggta cgtagtcaag ctgcacataa tttagatcaa ttttagttct tttttatatt
    79681 tctatgatct gctcatttac ttttatatga tggcttacag taacataaat aaatatgata
    79741 atcgctggta gtttatccaa gagtactcaa taaaatacaa aatggattgt tcaagcgcct
    79801 atcataatga taaaatgaaa caaataaacg atacaaggag gaagagactt tgagtaatgg
    79861 cagaacaatt ttttcattgt aaaaattgtt cagttgttaa tctatgggat ttcggtagca
    79921 tgggcatttt tttatagagg aatcacacca acctggccgt tttatctctt gttagtggga
    79981 atattagtag gaaattttgc ccctcttcac tatcgtacga acttttttcc aactgattct
    80041 ggcctttttc ttaaaacaaa gacgcgaaaa ttagaaaaca ttattgaaat gacaattacc
    80101 tgtataatca ttttcttagt tatattccta aacgtcttct aataagaatc tcttaaaatt
    80161 agcttgttac agttagttac ctagtttata taacacagtt atcttatctt ccttatcaac
    80221 atcttttgcc aactctttgt cttaaattcg aaacatataa gcagagctac gaacaccaag
    80281 actgtttcgg ctctgttttt tgcatcaaat tcactcttat atagctgata atctcggctc
    80341 tttaaaagaa attattttcg cttcatttta ttataaaaag gctaggaacc ttgatgttat
    80401 caggttccta gcctttattt aagtattacc ccacagaccc ttccatatag atttttaaat
    80461 tacacactag tttaaactaa aacaaacaag gaaaaactta ccatttaatc aaaaaattag
    80521 ctcatatgaa tatattagaa tgtaaattag actggtcaaa atctggtcaa aaaagaggac
    80581 gaatacaggt agtcctcttt tttgttattt tgatattcta aatcattttt tattggatta
    80641 ttcccttgtt cgatatagac ccaaattgat ctatcaataa ttatcaaact gagtcatata
    80701 gagacaaaaa tttcaaaaat gacctgttaa atggtcatcg aatatcattt aaaatcaata
    80761 cgacggagtc agacacggag tcaaaacaag cccgctgaga gtaaaaaata tacagtccca
    80821 taggtaaagt gccacaggac tgtatatttt tttatttgag attacctgaa atgattccat
    80881 ttaattttta tctcattttt tcctactagt tagtatcaaa tactctccta ttttctcaat
    80941 aaactatttt gggttacatg gtatctgaaa cagctaaaac cttgtcatta tagcaaaaaa
    81001 tcactttatc tccgtttcat ttcagataca tttcacttaa gcggattcca aaaaaacaaa
    81061 agaaaaagga acccctagtt agcctatgct ttttattggt tcctctcaaa acacggtcat
    81121 ataacaacgg ggtggggtaa atgaatgaaa aaggtggagg aaacattatt caatagctgt
    81181 cttaaatact tcgttataaa atgtgaacaa ataaaagaca tctatcccta ttttctacac
    81241 ttccacttga ctaccatctt tgaatcgtac aattttctta actcttctcg tttacctcaa
    81301 ttcattctca agtggaatca actcatcatc taacatttta cgccatgctc gcttcatttc
    81361 ttgtgggtat ttaaatggct tcgcttctgg atgtttggct ttatatcctt cgtaatcctt
    81421 actgttgata attgctttca tattaggaat tggatcttca cctggtagat attgcagatt
    81481 agaagcatgt aattctttct cagaaacaaa ccactcattg gaaaagacat gaatcaaatc
    81541 atcactcgct tgtgtaaaat agcgacgttt aatgacaaag atatcttcat tactatctaa
    81601 agtccccaca tcaaatgcat ttaaaatttg tcggtaaact tctttgactc cttctgtttt
    81661 attcaattta gccaaagctt tttcaatttc ttctcgtgca tctgaactat tcgcagcata
    81721 agttcctagt aattgatcaa tataagttga atcaatatca tacaacttag tggtattttc
    81781 actataaaat tcgatcgttg aaaaatcttc tccttctttt tctggttctt cctctattaa
    81841 tgaacccttc acggtctcgt acacgccaat ttgttcttcc aaaagttgaa tttgattgtt
    81901 caaagttgga gatttcacca tgtcatcatt gtaatcgtca taagtcacta acgattcata
    81961 agcacgattc aattcttgaa atgctttgac gtactcgata cggatttcaa gtggcgtatg
    82021 ttcatcaatg gattccatgg atgaaacctc ttttaactta ccatatgctt gtttgaatct
    82081 ttttttggat tcctgataac ttggataaat caaacctgat tcttcttttg cttttgaata
    82141 cagttttgtc gcatcctcga cattcttctc cattgtcgct ggcttacgaa acgtcacaat
    82201 caatcctttt gtcttatcag gatacgtacg attcgtacgt gaaaaagctt ggatcaatcc
    82261 tgcatactct agattacgat ccacaaatag ggtttgaatc gtaggggcgt caaaacctgt
    82321 taatagacga tccactacaa tcgctaaatc gatttgtttc ccaaattgtt tgaattccgc
    82381 tctttttcgt gccaaacgat tattgatgtc tccgttataa cgctcaatgt ctgctaatga
    82441 ccaggaagta tcataatact gattatattc ctcaataatt ttttgcattt cttcttgttg
    82501 ggcacttgaa tctcggctat tttcttctaa tgaataggta atagcgattc gtggaaaatc
    82561 aggatcctcc atcgttcgtc cttcacgaat gagctgattg ggaaactctt ttgttaacca
    82621 atcaggctct ttcgtcatct ctttgatggc ccgataatac tttttggcca tgtcaatgga
    82681 actcgtggtt aagattgcag atttcgttgg cctgccattt cgaaaatcaa attttgtata
    82741 ggcattatct ggacggaaca ttttatgaat gactttttgg atatgttcat ctgcttcaaa
    82801 aacagctggg tccaaataac tttctttttt gacaggttcc atttgatcaa tcatacgatt
    82861 gatctgttct gatgaatact ctgcatatgt ttctacttct tttaatttcc gatagatttt
    82921 attctctagt gaagtcggct ccacggtgtt ttcatgctct acttgaaatc ctaagacaga
    82981 gccatcttct agcgcattct taatggtata ggtatgcaag acctctccat actgatcatg
    83041 ggttgtacgt gccaattgcc ctttggcttg tttccgattc tcaggaaaaa tcggtgtacc
    83101 tgtaaagcca aaccaagtgg atttcggaaa catttttttg atttcttcca tattttcagc
    83161 acttaacgct cgatgacact catctactac aaacacgata tgttggccca ttaatttttg
    83221 gaagcgattg gttccttttt tctcttcttg tttctttgca tatttcaacg ccgcatctag
    83281 tttttgacgg gttgtaatga ttacaacatt tgcattggca tctgccaaca gggtttcact
    83341 taattctttg gcacttcccg ttccaacgat caagctattc gctttggcgt tcccggaaga
    83401 aatcccagta ttaaattccg aagcaaattt tgtaaactct gtggtggttt gattatctaa
    83461 atctttgcgg tccactaaca tgatcgttcg atcgatcccc gattttcgtg ctaaaagctt
    83521 agtagataca aaactagtca acgtctttcc tgaaccagtc gcatgccaca cataccctga
    83581 ttgatgttta tttgcggcaa taaacaaggc ttgaatcgca tgtacttgat aaggatgtaa
    83641 caccatgagg gttttattgt cttgatcttc actgacgatt gtataatccg caatcaaacg
    83701 atgagcatcc gggatattta acacttgttt acaaaactca tataagtttt ctactttttc
    83761 attgtctttc gttcgccaac taaataaaaa cttagggtgc aagtctttcg gtaaggcatt
    83821 tgcaaaatac cgagtggttt gttcattgga aaccacaaac aactgtaagg tcgaaaagat
    83881 attgttccga aacatacctt cttctgcata ttttttgatt tggttaaatg cctgataaac
    83941 accatctttt gcagttactt gtttcagctc gatttggaca atcggtaatc cattgattaa
    84001 taaggtgacg tcaaaacgtc gatcccgtgc ttctatgtta cttccacgtt ttgcaatttg
    84061 atggaccact tcataagtag agattcctcc accaatgtct tgattggaat aaaggataag
    84121 tgagacagaa cccaattgcg aatcttcccg ttcaatcgtg atccgagcca tcccattttc
    84181 accttttaac catttcgcag catcaaatgg ggttttcgtt cgcagcagta attccgtttt
    84241 gatcgtttca aattctttat cggtcaaagg aaaatcattc agttctgctt gattatttga
    84301 gataattttt tggcgtaaat tgacccaaag gtcttcttct gattttagat ccggacgata
    84361 cgtccactga ttatgccctt ctccaagtac acgaatcaac tgttcttcga cttctgcttc
    84421 ctcacggtga ctaattttca ttcttcttcc ctcctagaca aacatctttt gtaagaagct
    84481 ttttttcgtt tcttttagta aatctaactt acgttgatga agagcgatcg tatcatctag
    84541 ctgtttgaag aaagtaccga ttttttgttg ttcatccttt acaggaacct taacactgat
    84601 attctcaata tcagaactgt tataagaagg caatgcccct acttgggagt atcttggcaa
    84661 atttatagtt tcaaacaata ttttaccaaa atagatatcc caagtattat taaataaata
    84721 agccatggta ttgttatcta ttaagaaagg gcttgtgacc aaacgttttc tattaagcat
    84781 tattgctgcg ccaactttag caaatataat cgctggcaca tcttttattg gcttccattt
    84841 tttacgctca atttgctcat tagatacata atgatttgca tttctcattt catactcatt
    84901 accaatgtta ttcatgtcgg aaactttaaa aaatggagta ccttcagaac caccttgctc
    84961 ggcatcagga aatccaattc ccgattgagt tttacctaat tctcccaact tacgctcttc
    85021 ccaatcttcc gtaaaaccag gaaaccggat ttctggcact tttgctccgt tttcagggaa
    85081 cattttttgt aagaatcctt ttttcgtttc cttcagcaaa tctaacttac gctgatgaag
    85141 agcgatcgtg tcgtcaagtt gtttgaagaa ggtgccgatt ttttgttgtt cttcctcact
    85201 aggaaacatt gctactatat ttttaaactt ttttagagac agaacatgtt gacctgttcc
    85261 ctctgcttga atagataatt gtcgttggat tccattgtta atacgtctta agaagaagtc
    85321 ggaatcagca gattttaaag taaatacagg ataaaaatag cttataatcc ctctatcaat
    85381 aatgtcatta cggttaaaca caaacacatc attatcactt cgactacgga acgtgaaata
    85441 tcctctaggt agtacaaagt aaccaatatt attttctgta gttacttgcc gatttgcaaa
    85501 atagtcttct tgcaaaacta tccctttttg ttgagatgaa gttagcatag gaaattgatt
    85561 ttgtacactt gtcttttcaa cataatctac aacgaagtcc cccaacttcc tctgttccca
    85621 atcatctgta aatcctggga aacggatttc tggttgtgta tcattgctca tgtttgaaca
    85681 cctccagcgc accttgtaac cacgcttcat tttcaggtgt tgtttgcagg gaagagatag
    85741 cttctaaaag ttttttttct agcacttgct gttcttgtcg tatcttcttg atgtcatttc
    85801 cgacatgaac catatcaatt ggttcttctt cttcgaaagt atctacatag cgtgggatat
    85861 ttaagttata gtcattttct ttgatttcat caaaggttgc caaatgagcg tatttctcga
    85921 catcttttct ttcagcgtag gtttcgagga ttttttgaat gttttcttcc gatagtttat
    85981 tttgattttt ccctttaaca aattcacgac tagcatcaat gaataataca tctcgagttt
    86041 gacgattctt cttcaacaca atcaccgtcg ttggaatcga tgtcccaaag aacaagttcg
    86101 caggcatacc gatcacagca taaatactgc catcttctag taatttttga cggatgaccc
    86161 cttctgcagc gccacggaat aaaactccgt gtggtaagac gatggccatg gtacctgttt
    86221 ccttcaaatg atagaaccca tgaagaagaa acgcaaaatc tgctttggat tttggcgcta
    86281 atttcccata acgattaaac cgagaatcat ctaagaaagt cgtatctgct gaccaattag
    86341 ccgaataagg tgggttcatt actactgcat caaatgtgta aggttcatct gttggccagt
    86401 ccttattcaa cgtatcccca ttgcggagat tcatttcttc ggcgtcgaca ccatgtaaaa
    86461 tcaagttcat ttttgctaga ttgtatgtcg ttgtatttag ttcttgccca tgatatttaa
    86521 cgttatctgg gtgtgttaag tagttacgta cgtttaacat caatgaacct gaacccatcg
    86581 ttggatcaaa aacactaaag aatcgtcgtt ccttttgatc gagcgttacg atttgcgcca
    86641 tcatatcaga aaccatatga ggtgtgtaaa attctccggc tttcttccct gcttctgaag
    86701 caaattgact gatcagaaac tcatacgcat ccccgatcac gtccccatcg tgttctaaaa
    86761 catctacatc attcaatttc ttgatgactt cggtaatcgt cacatttcgt tgttgctcat
    86821 ctgttcctag tttttttgat tggagatcca catcatcaaa taacccatta aattgattgt
    86881 aggtagatgc caattgcaca aaggctttgt ttagatcgtt caattggaac gttgcttgtt
    86941 ttgcttgatc cgctaagacg ttgaaaagat actctggtgc aattgcatat cccaaaatat
    87001 caacgattgt ggcaatcaga tcttcttttg attcttcatc tgaaagtaat tcacgataca
    87061 acattgtttg tttgcttact gtgtcatatt cctctaagga ttcatctgct aaaagaacca
    87121 cttgttccaa caatcgatcg gataaatatt tatagaaaat cagtcccaat aaataatttt
    87181 tatattctga cgcatccatt ttactacgga gattatctgc cgcactaaat aatttttgat
    87241 ttaattctgc acccaaaaag atacactcct atctcttatt taaagttgct atataccctc
    87301 ttattttagc ataccccctt ttataaaaag caaaaaaaaa aagaaagaaa cttattcttg
    87361 ctaataaact acgcgaaagg agtctaacg
//
LOCUS       DAJSBN010000004        82782 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJSBN010000004 DAJSBN010000000
VERSION     DAJSBN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.004"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            51..440
                     /locus_tag="ONT86_000287"
     CDS             51..440
                     /locus_tag="ONT86_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HCR2351110.1"
                     /translation="MSPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIA
                     VENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTI
                     AFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            568..1251
                     /locus_tag="ONT86_000288"
     CDS             568..1251
                     /locus_tag="ONT86_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCR2351111.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1560..1633
                     /locus_tag="ONT86_000289"
     tRNA            1560..1633
                     /locus_tag="ONT86_000289"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1594..1596,aa:Arg,seq:tct)
     gene            1792..2151
                     /locus_tag="ONT86_000290"
     CDS             1792..2151
                     /locus_tag="ONT86_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HCR2351112.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2274..3905
                     /locus_tag="ONT86_000291"
     CDS             2274..3905
                     /locus_tag="ONT86_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCR2351113.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3967..4608)
                     /locus_tag="ONT86_000292"
     CDS             complement(3967..4608)
                     /locus_tag="ONT86_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR2351114.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4794..4870
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4890..5198
                     /gene="rplU"
                     /locus_tag="ONT86_000293"
     CDS             4890..5198
                     /gene="rplU"
                     /locus_tag="ONT86_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR2351115.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5225..5569
                     /locus_tag="ONT86_000294"
     CDS             5225..5569
                     /locus_tag="ONT86_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCR2351116.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5582..5875
                     /gene="rpmA"
                     /locus_tag="ONT86_000295"
     CDS             5582..5875
                     /gene="rpmA"
                     /locus_tag="ONT86_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR2351117.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6309..9116
                     /locus_tag="ONT86_000296"
     CDS             6309..9116
                     /locus_tag="ONT86_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2351118.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9307..9798
                     /locus_tag="ONT86_000297"
     CDS             9307..9798
                     /locus_tag="ONT86_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2351119.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9901..10890
                     /gene="manX"
                     /locus_tag="ONT86_000298"
     CDS             9901..10890
                     /gene="manX"
                     /locus_tag="ONT86_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR2351120.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10923..11726
                     /locus_tag="ONT86_000299"
     CDS             10923..11726
                     /locus_tag="ONT86_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR2351121.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11747..12661
                     /locus_tag="ONT86_000300"
     CDS             11747..12661
                     /locus_tag="ONT86_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR2351122.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12744..13118
                     /locus_tag="ONT86_000301"
     CDS             12744..13118
                     /locus_tag="ONT86_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCR2351123.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13257..13502
                     /locus_tag="ONT86_000302"
     CDS             13257..13502
                     /locus_tag="ONT86_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2351124.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13674..14738
                     /locus_tag="ONT86_000303"
     CDS             13674..14738
                     /locus_tag="ONT86_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR2351125.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            14984..15409
                     /locus_tag="ONT86_000304"
     CDS             14984..15409
                     /locus_tag="ONT86_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2351126.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15402..15851
                     /gene="nusB"
                     /locus_tag="ONT86_000305"
     CDS             15402..15851
                     /gene="nusB"
                     /locus_tag="ONT86_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR2351127.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            15948..16802
                     /locus_tag="ONT86_000306"
     CDS             15948..16802
                     /locus_tag="ONT86_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCR2351128.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16803..18140
                     /locus_tag="ONT86_000307"
     CDS             16803..18140
                     /locus_tag="ONT86_000307"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR2351129.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18145..18369
                     /locus_tag="ONT86_000308"
     CDS             18145..18369
                     /locus_tag="ONT86_000308"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR2351130.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18371..19261
                     /locus_tag="ONT86_000309"
     CDS             18371..19261
                     /locus_tag="ONT86_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR2351131.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19270..20088
                     /locus_tag="ONT86_000310"
     CDS             19270..20088
                     /locus_tag="ONT86_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCR2351132.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20195..20644
                     /locus_tag="ONT86_000311"
     CDS             20195..20644
                     /locus_tag="ONT86_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR2351133.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20662..22344
                     /gene="recN"
                     /locus_tag="ONT86_000312"
     CDS             20662..22344
                     /gene="recN"
                     /locus_tag="ONT86_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR2351134.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22399..22515)
                     /locus_tag="ONT86_000313"
     CDS             complement(22399..22515)
                     /locus_tag="ONT86_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCR2351135.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22588..23544)
                     /locus_tag="ONT86_000314"
     CDS             complement(22588..23544)
                     /locus_tag="ONT86_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR2351136.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23715..24083
                     /locus_tag="ONT86_000315"
     CDS             23715..24083
                     /locus_tag="ONT86_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCR2351137.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24200..24342
                     /locus_tag="ONT86_000316"
                     /pseudo
     CDS             24200..24342
                     /locus_tag="ONT86_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719784.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
     gene            24366..24797
                     /gene="mraZ"
                     /locus_tag="ONT86_000317"
     CDS             24366..24797
                     /gene="mraZ"
                     /locus_tag="ONT86_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR2351138.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24818..25777
                     /gene="rsmH"
                     /locus_tag="ONT86_000318"
     CDS             24818..25777
                     /gene="rsmH"
                     /locus_tag="ONT86_000318"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR2351139.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25782..26183
                     /gene="ftsL"
                     /locus_tag="ONT86_000319"
     CDS             25782..26183
                     /gene="ftsL"
                     /locus_tag="ONT86_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR2351140.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26184..28376
                     /locus_tag="ONT86_000320"
     CDS             26184..28376
                     /locus_tag="ONT86_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR2351141.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28402..29364
                     /locus_tag="ONT86_000321"
     CDS             28402..29364
                     /locus_tag="ONT86_000321"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR2351142.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29378..30739
                     /locus_tag="ONT86_000322"
     CDS             29378..30739
                     /locus_tag="ONT86_000322"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR2351143.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30765..31853
                     /gene="murG"
                     /locus_tag="ONT86_000323"
     CDS             30765..31853
                     /gene="murG"
                     /locus_tag="ONT86_000323"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR2351144.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31868..33088
                     /locus_tag="ONT86_000324"
     CDS             31868..33088
                     /locus_tag="ONT86_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCR2351145.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33244..34569
                     /gene="ftsA"
                     /locus_tag="ONT86_000325"
     CDS             33244..34569
                     /gene="ftsA"
                     /locus_tag="ONT86_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR2351146.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34592..35833
                     /gene="ftsZ"
                     /locus_tag="ONT86_000326"
     CDS             34592..35833
                     /gene="ftsZ"
                     /locus_tag="ONT86_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR2351147.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35850..36527
                     /locus_tag="ONT86_000327"
     CDS             35850..36527
                     /locus_tag="ONT86_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCR2351148.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36541..37140
                     /locus_tag="ONT86_000328"
     CDS             36541..37140
                     /locus_tag="ONT86_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCR2351149.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37155..37442
                     /locus_tag="ONT86_000329"
     CDS             37155..37442
                     /locus_tag="ONT86_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCR2351150.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37491..38273
                     /locus_tag="ONT86_000330"
     CDS             37491..38273
                     /locus_tag="ONT86_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCR2351151.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38294..39001
                     /locus_tag="ONT86_000331"
     CDS             38294..39001
                     /locus_tag="ONT86_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCR2351152.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39263..42061
                     /gene="ileS"
                     /locus_tag="ONT86_000332"
     CDS             39263..42061
                     /gene="ileS"
                     /locus_tag="ONT86_000332"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR2351153.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42110..43636)
                     /locus_tag="ONT86_000333"
     CDS             complement(42110..43636)
                     /locus_tag="ONT86_000333"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR2351154.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43651..44298)
                     /locus_tag="ONT86_000334"
     CDS             complement(43651..44298)
                     /locus_tag="ONT86_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCR2351155.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44482..44811)
                     /locus_tag="ONT86_000335"
     CDS             complement(44482..44811)
                     /locus_tag="ONT86_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCR2351156.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44988..45716
                     /gene="sfsA"
                     /locus_tag="ONT86_000336"
     CDS             44988..45716
                     /gene="sfsA"
                     /locus_tag="ONT86_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR2351157.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45732..46745
                     /locus_tag="ONT86_000337"
     CDS             45732..46745
                     /locus_tag="ONT86_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2351158.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46745..48022
                     /locus_tag="ONT86_000338"
     CDS             46745..48022
                     /locus_tag="ONT86_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR2351159.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48085..50787
                     /locus_tag="ONT86_000339"
     CDS             48085..50787
                     /locus_tag="ONT86_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2351160.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50939..51256
                     /locus_tag="ONT86_000340"
     CDS             50939..51256
                     /locus_tag="ONT86_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2351161.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51286..51606
                     /locus_tag="ONT86_000341"
     CDS             51286..51606
                     /locus_tag="ONT86_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR2351162.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51714..53174
                     /locus_tag="ONT86_000342"
     CDS             51714..53174
                     /locus_tag="ONT86_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2351163.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53242..53463
                     /locus_tag="ONT86_000343"
     CDS             53242..53463
                     /locus_tag="ONT86_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351164.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53494..53676
                     /locus_tag="ONT86_000344"
     CDS             53494..53676
                     /locus_tag="ONT86_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCR2351165.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53676..54089
                     /locus_tag="ONT86_000345"
     CDS             53676..54089
                     /locus_tag="ONT86_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCR2351166.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54212..55393
                     /locus_tag="ONT86_000346"
     CDS             54212..55393
                     /locus_tag="ONT86_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2351167.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55464..55628)
                     /locus_tag="ONT86_000347"
     CDS             complement(55464..55628)
                     /locus_tag="ONT86_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351168.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55782..55855
                     /locus_tag="ONT86_000348"
     tRNA            55782..55855
                     /locus_tag="ONT86_000348"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55816..55818,aa:Arg,seq:cct)
     gene            complement(55892..56092)
                     /locus_tag="ONT86_000349"
     CDS             complement(55892..56092)
                     /locus_tag="ONT86_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2351169.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(56397..57629)
                     /locus_tag="ONT86_000350"
     CDS             complement(56397..57629)
                     /locus_tag="ONT86_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR2351170.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57883..58452
                     /locus_tag="ONT86_000351"
     CDS             57883..58452
                     /locus_tag="ONT86_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2351171.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58630..59070)
                     /locus_tag="ONT86_000352"
     CDS             complement(58630..59070)
                     /locus_tag="ONT86_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR2351172.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59228..59992
                     /gene="map"
                     /locus_tag="ONT86_000353"
     CDS             59228..59992
                     /gene="map"
                     /locus_tag="ONT86_000353"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR2351173.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            60024..60947
                     /locus_tag="ONT86_000354"
     CDS             60024..60947
                     /locus_tag="ONT86_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCR2351174.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            61023..62162
                     /locus_tag="ONT86_000355"
     CDS             61023..62162
                     /locus_tag="ONT86_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCR2351175.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62155..62955
                     /locus_tag="ONT86_000356"
     CDS             62155..62955
                     /locus_tag="ONT86_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2351176.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            62955..63782
                     /locus_tag="ONT86_000357"
     CDS             62955..63782
                     /locus_tag="ONT86_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2351177.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63760..64494
                     /locus_tag="ONT86_000358"
     CDS             63760..64494
                     /locus_tag="ONT86_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2351178.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            64594..65460
                     /gene="rfbA"
                     /locus_tag="ONT86_000359"
     CDS             64594..65460
                     /gene="rfbA"
                     /locus_tag="ONT86_000359"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR2351179.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65474..66046
                     /gene="rfbC"
                     /locus_tag="ONT86_000360"
     CDS             65474..66046
                     /gene="rfbC"
                     /locus_tag="ONT86_000360"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR2351180.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            66068..67096
                     /gene="rfbB"
                     /locus_tag="ONT86_000361"
     CDS             66068..67096
                     /gene="rfbB"
                     /locus_tag="ONT86_000361"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR2351181.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67194..68045
                     /gene="rfbD"
                     /locus_tag="ONT86_000362"
     CDS             67194..68045
                     /gene="rfbD"
                     /locus_tag="ONT86_000362"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR2351182.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            68080..70113
                     /locus_tag="ONT86_000363"
     CDS             68080..70113
                     /locus_tag="ONT86_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351183.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70157..71437
                     /locus_tag="ONT86_000364"
     CDS             70157..71437
                     /locus_tag="ONT86_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCR2351184.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            71647..72453
                     /locus_tag="ONT86_000365"
     CDS             71647..72453
                     /locus_tag="ONT86_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2351185.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            72465..73685
                     /locus_tag="ONT86_000366"
     CDS             72465..73685
                     /locus_tag="ONT86_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2351186.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            73675..75261
                     /locus_tag="ONT86_000367"
     CDS             73675..75261
                     /locus_tag="ONT86_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2351187.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            75300..77438
                     /locus_tag="ONT86_000368"
     CDS             75300..77438
                     /locus_tag="ONT86_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2351188.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(77806..78789)
                     /locus_tag="ONT86_000369"
     CDS             complement(77806..78789)
                     /locus_tag="ONT86_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR2351189.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            79167..80558
                     /locus_tag="ONT86_000370"
     CDS             79167..80558
                     /locus_tag="ONT86_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR2351190.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            80574..81614
                     /locus_tag="ONT86_000371"
     CDS             80574..81614
                     /locus_tag="ONT86_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2351191.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            81633..82718
                     /locus_tag="ONT86_000372"
     CDS             81633..82718
                     /locus_tag="ONT86_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCR2351192.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT        27063 a        13940 c        17543 g        24236 t
ORIGIN      
        1 tggttccccg aatttttggt ttgactcctt atattattga ggacaaagaa atgagtccat
       61 attatgcaca aggaaatctg atatatgtac gaccgaaaaa tacaaatgag attttagtcg
      121 gagatattat tacatattat gaaaattcag gaaaaaaaat ctcgacgcgt cgcgtaatag
      181 ccgttgaaaa caaccacgaa gatttttata caaaagctga tacaaagact tatattgagc
      241 ctggagtagt aaaaaagaga aatattatcg gtagtcccat ctttcagatt ccatatattg
      301 gactagtcct ttcaaaaacg gcatttgcat ggattcaggg gttattcttt acgattgctt
      361 tttgcctaac aggaatcaca gcatggaata cctttgtaga gttgaaaaag aagcggagac
      421 gaacacagaa atttttgtaa gaatgttaaa aacataaatg tattccagag atccaacgac
      481 tcttctgaac atgaaacacg agaaaacact catgcagctt gtagatttag tcagttgttc
      541 gtataatgtg tgaagaggag gagtagtatg cgcaatgaaa tgatgcatcg tcctgtagtc
      601 aaagaagaac tattggattt tatgcgtacc cagcaaaaaa aattaccggg tgagctaggc
      661 aaactggaag aagaagctca tgtagcagaa gtaccagtta ttccccatga aacagttgta
      721 tttttgaaat ttttacttgg acagcttcag cctgaacgaa tcttggagat cggagcagct
      781 atcggttttt catcaagtgt catggccacg acaatcggag aaaacgctca tgttacgaca
      841 attgatcgtt tcgatgtcat gatcgagaaa gctaaaaaaa actatgaacg tctaggactg
      901 acagataaag tgactttgct tgaaggtcaa gcagcagata tcttgccgga gttgtctggt
      961 ccgtatgact ttatttttat ggatagtgca aaatcaaaat atattgaatt tttacctgaa
     1021 tgcttacgtt tgttaagaaa aggtggcgtt ttgatggtag atgacatctt ccaaggagga
     1081 acgatcttgc tcccagatga agagattccc cgtggcaaac gagctatcca tcgtaaatta
     1141 aatgaatttc ttcgtgttgt gatggatcat cccgatttga catcgacaat cttgccatta
     1201 ggtgatggtg tgatcttaat gacaaaggaa tcagaaacaa tcgagttgtg atacatgatt
     1261 atggcagcct gtagataatc gattgaatcg agggctcaat tcgaacttga tgtaaaagtt
     1321 tctatatttg taagtcaatt aaaggataca ggaaatctaa ataaacagga gtagcatgaa
     1381 gtcaatgtag ctcctgtttt caattgataa ccttagaaca gctgaaggag aatgtctcgt
     1441 tttaaaagat tgatttagta gttgggccaa aataattcat tatttgctaa ttaaataact
     1501 atttcgtatt gacagaagca atttaattat ctataattat tgatgttccg ctgattcttg
     1561 tcatagtagc tcagctggat agagcatccg ccttctaagc ggacggtcgg gggttcgaat
     1621 ccctcctgtg acgctaaaaa accgttagaa actgaggttt ctaacggttt ttctttgttt
     1681 tttgtgccaa tattgtttgt tttcaaaggc aaataaaata aaaaaacatc cttatcgagt
     1741 acaattataa ggagcaaaaa aataaaactc atcagaaaag aggaagaaga tatgacgata
     1801 ctatctagaa ttttagtgac tctagttgca attgaatttt tttatatcat gtatatcgaa
     1861 acggtgagaa ctgattctga cacgacaagt agggttttta aaatgagtaa agaagagtta
     1921 agcagaaaat ctgttcaaac attgtttaag aaccaaggag tgtacaatgg actattagga
     1981 gtgggactac tttatggagc ttatctttct tccgcatcta aagaaatcac aagtatgcta
     2041 ttaatatcta ttttttttgt tgcattatat ggcagtttga ctagcgatcg ttggatttta
     2101 ctcaaacaag gtgggctagc tctgttggca ttgctttcta ttttcttata aatatagaaa
     2161 aaataagaat ttatctaaca ggaaactttg tgaaaaatat tactttatct catgaaatat
     2221 gatattctaa tgaatggtat tttagaaaat ttcatgaaaa aggagttaga taggtgttca
     2281 aaaattatgt ttcctcacta aaaaaagaat tttcaggtta tgatctttca agatttcaga
     2341 aagatctttt ggctgggatc acagtagctg ctgtagcatt gcctttagcc ttggcttttg
     2401 gggtatcaag cggagctgat gcggccgcag gtttgattac ggctatcatt gccggtatcg
     2461 ttattggcgg tttatcaggc ggtttttatc aaatatcagg tccgacaggt gcgatggcag
     2521 ctatcttgat gtctattgcg gcaacaaagg gaatgcaagg tgtattgctt gctacttttt
     2581 tagcgggtct atttttactt gcagcaggac ttttccgatt aggaacactg acttctttta
     2641 tcccagcacc cgtaattacg gggtttactt caggtattgc tatcattatt gcattaggtc
     2701 agatcgataa tttatttggt gttcactcgg aaggtacaaa cgttattgaa aaactaagta
     2761 gttatcaaac gataggattt ccgataaaca cagcctcttt gatcatggga gggctagtca
     2821 ttttagggat gtttatctat ccaaaaaaat gggcgaaacg aatgcctagt tcgctgctgg
     2881 ctatcatttt ggcaacagca gttatgctat tgttccattt accagtagca actgtgggta
     2941 agattccaca aacattgatc agtagtaatc gtttgaatat gggagatttt agcttttctg
     3001 ccttgcaaca agtggcagtt ccagctatta gcattgcttt attaggaatg attgaaagct
     3061 tgctatgtgg tgcttcggct ggccgaatgg caaataagcc cttagatagc aatcaagaat
     3121 taatggcgca aggaattgga aatctgttgc ttcctttctt tggcgggatt cctgctactg
     3181 cggccatcgc ccgtacaagc gtggccatca aatctggtgc acaaactaga ttagctggta
     3241 tgatacatgc agttgtctta ctgctctcca tgcttgtttt tgcgccagtg atgtcacaga
     3301 ttccaatgcc agcacttgca ggtgttttga ttgtgacggc ttggcgtatg aatgaatggg
     3361 aaacgatcaa ggagctattt actaataaat attggtcagc agttctattg tttatcctta
     3421 cgatgatttg tacagttatc tttgatttaa gtattgctat cgtgatcggg gtgatcggcg
     3481 gctgtgtatt ttttgttgtc aaaagcgctg ctatcacgat ttcattagaa acgatcgatt
     3541 ggcaaagaat ggggctgcca gaaacggaaa agctgaataa ctggacagta gtgtatatta
     3601 gtggtccact gttttttatg tcttcagaaa aactcaaaaa taaattggaa gaactaaatc
     3661 atcacgatgg tgtcattttc tccatgcgag gcgtaccaag tattgatttg acggcacgtg
     3721 cagtttttga tgaatttcaa gaaaaagcag agcaaagagg acaaacgatt atctatactt
     3781 ccttgcagcc agaagtagaa aaacaacttt ctcacttgtg ggaacataca ttcacgcatc
     3841 aacatcaaac agttgctcat gcgttgacag ctttacatgc gaaatatact gcaattagcc
     3901 attaaaacaa tgaaaaagaa cagacaagac tgtaaactga agattttatg gtcttgtctg
     3961 ttctttttaa tattgctgta aaagtgaagc tagtacttgc cagtcttgat ggataacagg
     4021 tacaagggag cgattgtaga aaatagctgc tggatgaaaa agtggaagaa ttcgatagtt
     4081 tttttctgac cattcgtagg catccttttg ttctgttaat tgtaagacag gccctgtata
     4141 gagttgacca tgatttttgg taaccgtata ttctttgcca agtaaacgtt gaagaccaat
     4201 gtttccaagt gtcgcaatta attttggctg gaccttcatc agttcgtaat ccaatattgg
     4261 tgcatgcgca aatacttcgg aacgagtagg tgtccgattt ggataaacag tttctgactg
     4321 ttgtgtccgt ttattgatcc gatgagtcac gcgatacggg cggctcctta cagcactggt
     4381 gatatacacc tcttctcgtg ttaagccagt tgaagaaagt gcctgcatca attcttttcc
     4441 agcttgaccg ctaaaaggaa catgcgtatc aatctctgtt ttccctgggg cttctcccac
     4501 gagcatcagt ttaggatgaa gaggtccttg cccagcgata aatccttcta caggacggcc
     4561 gttcattctt aaagaagctt cttgtatcag ttcttttggg tattccattt atgctcctcc
     4621 tttttctttt attataagaa taaaagaaac acaatgcgac tattttattt ggtagaactg
     4681 tgaggataga aaagcagaag tattgcttga atttttgttt tactttttga ttttaaattt
     4741 agttgactaa cggttgctgt tctggtaagc tagtactgtt gtaattgtgg actccacagc
     4801 tacaaccgct cagaacaagt aataagcaca gcatatgctg tcacttcgga tggcgagtct
     4861 aagtatatca gaaggaggtg ctttatagca tgtacgcaat tattaaaact ggtggtaaac
     4921 aagtaaaagt tgaagtggga caagcaatct atgttgaaaa acttgacgtt gaagcaggag
     4981 aaaaagttgt ttttgacgaa gttatcttag taggcggaga atctacaaaa gtaggcgctc
     5041 caactgttgc aggtgcaact gttgaaggta ctgttgaaaa acacggcaaa caaaagaaag
     5101 tcgttacttt caaatacaaa cctaaaaaac attcacaccg taaacaaggt catcgtcaac
     5161 catatacaaa agtggttatc gacgcgatca acgcttaata gcgtttgaga aagaaggcct
     5221 atatatgatc aaaggaacat ttaagcgaaa tgacgcaggt cagatcgttt cattcacttt
     5281 gaccgggcat gctgatgctg gtccttatgg aagtgatatc gtttgtgcgg gtgtttctgc
     5341 gttagcgatc agcactgtca acgggattgc ctcattagcc ggttttgagc cgattgttga
     5401 aatgaatgaa gaagaaggcg gttatcttta taccgaagtc acttcaggaa tgacacaaga
     5461 acaaaacaat attgctgaga ttctattaga aaatctttta ttaggattac aatcgataga
     5521 agctgaaaac tcagaatata tccaaattaa aacaattaat gataaatagg aggtgcagac
     5581 catgttgtta tctatgaatc tacaattatt tgcccacaaa aaaggtggcg gttctacatc
     5641 taacggccgt gactcagaat caaaacgtct aggtgctaaa cgtgctgatg gacaaacagt
     5701 tacaggtgga tcaattcttt accgtcaacg cggtacaaaa atctatccag gtgtaaacgt
     5761 gggtatcggt ggagacgaca ctttatttgc aaaagttgat ggcgtagtac gtttcgaacg
     5821 taaaggccgt aacaaaaaac aagtgtctgt ttatccagta gctcaagaag cttaattgaa
     5881 tcttatgatt aaagaggttg tgacaagact tttgtcacag ccttttttgt ttataaaaat
     5941 aaaagttgta gtggaggcta cgaagtttac tttgtctttt tacacttaat atttttttgc
     6001 caatttggaa tcaggatgtt ttgataacta tcgttttcaa ttgatggaag ctatattgtt
     6061 tgctatcagt gaaataatta tatggtaaac agtgttcttt ttcttgtttc cattataaaa
     6121 atcgtatcgg gatttttcgt tcttttaaat tgaacatttc tatctatccg cgtgttatca
     6181 acaaaacaaa atcaggtaaa tagttgacga aacagaaata aatacatagt attatgtttt
     6241 gaagcctaag tacaatagtc tatagaaaag gagcgatacc attggcaaaa cgcattgacc
     6301 gaatttatat gtatgtcaaa gaaaaaaccg ctcatttaac aaaggcagaa tatgatcaag
     6361 gagtaacaac acaggaaatt gctgaagtgt tagggattca gcgaacaaat agcagtaaag
     6421 atctgaatca gctagtgcgc gaaggaaaac tgataaagac agacggacga ccagtacgtt
     6481 acatatatca aacactcgtt acgcacgtaa agccagttac aaaacatgta gtcagttaca
     6541 aagaaacgcc ggtctctgaa gaaaaaaata cactccaaat agatactaag gatatctttg
     6601 caaaaatcat tggagctaac ggcagcatga aaaattcagt ggaacaggca aaagctgcga
     6661 ttttgtatcc gccaagagga ttaaattgtt tgattacagg acctaccgga tcaggaaaga
     6721 cttattttgc acacgcgatg tatcattttg caaaagcgca tcatgtaata gatgaggaaa
     6781 cggaactgat tgttttcaat tgtgctgatt atgccaataa ccctgagctt ttgatgagtc
     6841 atttatttgg gtacgcaaaa ggtgctttta caggcgcaga tgaagaaaaa atggggatta
     6901 tcgatcaagc ggatggcggt atgctgtttt tggatgagat tcaccggttg ccgcctgaag
     6961 gtcaagaaat gatcttttac tttatggatc atggaactta cagtcgtctt ggggaaacaa
     7021 cgaaatccca tgaagcaaat gtacggatcg taggtgcgac cacagaagac ccaggttctt
     7081 ctctattaga aacttttgtt cggcgtatcc caatcaatat caaacttccg gcttttgaaa
     7141 aacgtcctgc aaatgagaaa gtcgatttag taaaaatcat gattgcccac gaagcaaacc
     7201 gcacgcagcg aaagatttct ttgacagaag atgtagcaaa agcattgatc ggcagtgtga
     7261 cctatggaaa tataggacaa ttgaagtcaa atatccagtt agtatgtgca cgaggatttc
     7321 taaatcatat gcaatcaccc gaaatctcga tcacaatcga tgatttgaca gaagggattc
     7381 gtagcggatt gattcaatta gcaagcaacc gcagtgcaat ggcagagctt tctaaaatat
     7441 tggagccaaa gataactgta gcaccaaatg atacgatcat gaaaatccaa tcggactcct
     7501 atgagttacc ttataatcta tatgatatca tcggagataa agcagcttta ctgaagtcgg
     7561 atggtttgga tcaagaagca atcaatcatt tcatttctac agatatcaac atccatctga
     7621 agtctttcta taaagaccat ggtttttctt ttaatgcaga cagcaaatta gcagaatttg
     7681 tagatccgaa aatcatagaa gtgaccaatc agatttatgt aatggtcaaa agatctttac
     7741 catatgagtt tcagcaaaac tttatttatg ctatgagctt gcatatcagt tcttttttga
     7801 aaaaaatcaa cttaggagaa gaacggcata cgaatgataa catccgtgag atggcttttg
     7861 attttccaga tgaatatgct gttgcgaaag aagtgcgtag ttatatcgaa acgtattttc
     7921 aagtggagat tccagaaagt gaagactatt acttgaccgt tctactcgtc tcgttacggg
     7981 caaatcaagc aagcggcaga atcggtgtag tcgttgcagc tcacggaaac agcacagcaa
     8041 gcagtatggt ccaagttgtc caacagttgt tagatgcaga gaacgtacga gcagtagaca
     8101 tgcctttgga tatggatccg aaaacagcat tggaccgaat tgaacgcaat gtacaagaag
     8161 tagatgaagg aagcggggtc atactcttag tagatatggg gtcccttgcg tcgtttaatt
     8221 cgcaaattca aagagacact ggaattccag tacgtactgt tgatatggta acaacttctc
     8281 tagttttgga aactgtacga aaggtctcag tattagggac agatctagat atgttgcatg
     8341 aatcattgaa aaattttcga ggttacgcag aaatatcttc tgtagaatct gttcaggaag
     8401 caaaacctat tcataagaaa aaagctgttt tggctatctg cgcatctggc gaaggtacag
     8461 cacaaagaat caaagaattg atcgaacgag cggtaaacaa gcgtcaagaa acagaattag
     8521 aagttctagc tttgtcgatt gtggaagtga aagaagaact gccagttatc caaaaagaat
     8581 atcagatcat agctactact gggattactg atttgaatat ccaagcccca tttattcctt
     8641 tagaacgttt tattgatcaa aatatcgaag tcattttgga tcagttgctg atggaatcag
     8701 aattggagga aactgaattc atctccctag atgaagagtc agctaaaaat acgtgtgttg
     8761 agtttatctc tgataacttc atctttatta atggtagtaa gttaatcgat ccaatgtggc
     8821 aattttctac acaaatctct cagacgacgg gaatcggcga tgaagaatat ggattcaaaa
     8881 tcaacctagt gatgcacaca gcaggaatga tcgaacgtat tattcggaat gaaccattga
     8941 ctgtggaaga aaacgaattg acaaatacaa cgaatgatcc tctttatagc caattggcag
     9001 cctcagttgt tctgttggaa gatcagataa aagtaaaagt gccgatagaa gaaatgtatt
     9061 atctgctacg tctagtccat aatcagttag ataaaaaaga atacactgtg ccatgaatac
     9121 acaaaaacga tacactttta tagtgtatcg tttttgtgta ttggttaaga atcctttaaa
     9181 ttcaattagt gtatcgtaaa aaagttggca cgctttttgc ttatagtagt gtgtgctagg
     9241 aggtggttgt tcacacagaa atgatagggg tttctgtgaa gtagtgagga agaaggagtt
     9301 ttgcagatgg atattcgatt ggcacgtata gatgatcgtt taatccacgg gcaagtcgca
     9361 acagtttggt cgaagcaaac aggtgttgaa cgtatcattg tgatcagcga tgtagtcgct
     9421 caagataaat tgagaaagtt tctactaaaa gaagctgcac cgccggggat cacagcaaat
     9481 gtaataaccg tgcagaaaat gattcaaatc tatcatagtg gattattatc acgagcaaaa
     9541 gtgatgcttt tattcaccaa tccccaagat gttgaagcgg ttgtccgctg cggtgtacag
     9601 ttgaagtcgt tgaatatcgg cggtatgagt tttactgaag gcaaatcaat gattaccaat
     9661 tttgtttcag taaatcaaca agatattgat tcctttcgtt acttgaacag tcaagaagtg
     9721 gagttagaga ttcgaaaagt acctgctgac aggaaaatcc ttttgatgga actactagcg
     9781 aaaatcaaaa aggtgtagca agaatggttg ttggaaaagg tcaaataatt ctgttgtaat
     9841 ggttagcaat tattaatatt tttgaatttg ttacaacaaa agtaaatagg aggtataaat
     9901 atggtaggga ttatcctagc aagtcatgga caatttgctg aaggaatctt acaatccggt
     9961 tcaatgatct ttggcgaaca agaaaacgtc aaagccgtga ttttgaaacc aagtgaaggt
    10021 ccagatgact tgagagccaa attggaagaa gcggttgctt cttttgataa tcaagacgaa
    10081 gtgttattct tagttgattt atggggcgga acaccgttca atcaatcaaa cacattattc
    10141 gaagagcaca aagataaatg ggcaatcgtc agcggtttga atttaccaat gttgattgaa
    10201 gcctacgcat cacgcttttc aatggaatct gctcatgaaa ttgcagcaca catcattgaa
    10261 acagcaaaag acggtgtaaa agtaaaaccg gaagaattag aaccggcaga agcaccgaaa
    10321 gcagcagttg aagacgcaca acctaaagga gcacttccag aaggaactgt ggtcggagac
    10381 ggaaaaatca aatatgtatt agcacgtgtt gactcacgtc tgttacatgg acaagttgcg
    10441 acagcttgga caaaagcagt gcaaccaaac cgtattatcg ttgtttcaga tgcagtatca
    10501 aaggatgatc tgcgtaagag actaatcgaa caagctgctc ctccaggtgt aaaagcaaat
    10561 gttattccta ttagcaaaat gatcgaagta gcaaaagacc ctcgttttgg aaataccaaa
    10621 gcattgcttt tatttgaaaa tcctgaagac gtattgacag ctgttgaagg tggcgtagac
    10681 atcaaagaat taaatgttgg gtcaatggcg cattcggtcg gtaaagtcgt tgtaagtaaa
    10741 gtattgtcta tgggacaaga agacgttgaa gcgtttgaga agcttgaaca aaaaggtgtg
    10801 aaattcgatg tacgtaaagt acctaatgat tcacgcgata acatggatga tattttgaaa
    10861 aaagcaaaag ctgaattagc aaaagcgtaa taaataatat taaaatttat aggaggctta
    10921 tcatgtctat tatttcaata attttagtcg tattagttgc ctttctagct ggtatggaag
    10981 gtattcttga tgaatttcaa tttcatcaac cactagtggc atgtacattg atcggtttag
    11041 ttaccggtaa cctcgaagct ggtatcgtac ttggcggtac gcttcagatg atcgctttag
    11101 gttgggcaaa tatcggtgcc gctgtagcac cagatgctgc attagcatca gtagcatcag
    11161 caattatttt agttttaggt ggacaaggtg ttaaaggtgt tccttctgcg atcgcgattg
    11221 ccgttccttt ggctgttgca gggttgttct taacaatgat cgtacgtaca atcgctgttc
    11281 caatcgttca tttgatggat gcggctgctg aagaaggaaa catccgtaag gttgaaatgt
    11341 ggcacatcat tgctgtatgt ttacaagggg ttcgtatcgc ggtcccagca gcagcgttgc
    11401 tatttatccc agcacaaact gtgcaatcat tccttgaatc aatgccagca tggttaacag
    11461 atggtatggc tatcggtggg ggaatggtcg tagctgtagg ttacgctttg gtaatcaaca
    11521 tgatggctac aaaagaagta tggccattct tcgttattgg tttcgttgta gctgcaattt
    11581 ctcaattaac attgattgct ctaggtgctt taggtgttgc attagctctt atttacttga
    11641 acctttctaa aatgggtggt tcatcaaata atggaggcgg aagcaactct ggtgaccctt
    11701 taggcgacat tttgaatgac tattaatctt gaaggaggaa aataaaatgg cagaagaaaa
    11761 aatcaaatta tcaaaaaaag atcgtttagc tgttgcgtgg cgctctacct tcattcaagg
    11821 ttcatggaac tacgaacgta tgcaaaacgg tggctgggca ttttctatga ttccagcaat
    11881 caaaaaatta tacaaaacaa aagaagaacg ttctgcagca ttaaaacgtc acttggaatt
    11941 ctttaataca cacccataca ttgcttctcc aattttaggg gttacattgg ctctggaaga
    12001 agaacgtgcg aatggcgcgc ctgttgatga tgtagcgatt caaggggtta aagtaggtat
    12061 gatgggtcct ttagctggtg ttggtgaccc agtgttctgg tttacagtac gtccaatgct
    12121 aggtgcacta ggtgcttcat tagctatggg aggaaatatc cttggaccaa tcatcttctt
    12181 cttaggttgg aacattattc gttgggcatt catgtggtat acacaagaat ttggctacaa
    12241 agctggttct aaaattactg atgacctttc tggcggattg ttacaagacg taacaaaagg
    12301 tgcttcaatt ttagggatgt tcgtattagc tgcccttgta caaagatggg tatcaatcaa
    12361 attcttacca gttgtatcaa ctgtaaaatt agataaaggt gcttacatcg aatgggataa
    12421 attgccttca ggtggtgaag gaatcaagag tgcatttgaa caagttaata atggcttagc
    12481 cctttctcct gaaaaggtaa caacattaca agacaactta gaccaattga ttcctggttt
    12541 agctgcatta ggattaacat tgttatgtat gtggttgctt aagaaaaagg tttctccaat
    12601 cgttatcatc cttggtttat tcgtagtcgg tgttgttgga cacgtcatcg gacttttata
    12661 atccataaaa atatagctcg ggcgttgtcc cgagcttttt ttatatagta tactgttcat
    12721 aaacaaatat ctggagggag aaaatggttc aatcaatcaa tacaaaagta gacttggtaa
    12781 ttgatgctac ttcgtttacg ggtctgacag attacgggca aatcatgata ggtgataatg
    12841 gttttgagtt ttaccatgcg cgagatagac gcaaatatat ccaaattcca tgggaagaag
    12901 tggattatgt actagcatca gtcatgctaa agggaaaatg gattcctcga tatgccattc
    12961 aaacgaaaaa aaatggtaca tacacatttt cttccaaaga acctaaaaaa gtgcttcgtg
    13021 cgatcagaga atatgtagat gaagatagaa tggtacaatc attgagtttc tttgatgtag
    13081 tgaaacgtgg tattcgtagt atgtttaaac gcaagtaaaa aataaagaaa acttctaaga
    13141 ggaggttttc tttatttttt tactttgcag aagaaaatat gaaatagcaa aaccaattat
    13201 ttgtcccctt taaatcccat atgctaaaat tgattagcaa agaaggaggt tatattatga
    13261 gttggttatg gacattgatt gttggagccg tgatcggagc aattgcaggt gcgatcacca
    13321 gtaaaggaaa ttcgatgggc tgtgtaacga atatcattgc tggactagtc ggttcaagta
    13381 ttggacaagc gattttcggc gaatggggac caaaagctgc aggtatggca cttattccat
    13441 ctattttagg cgcagtgatt ttggtagcag ttgtctcatt tttctttgga aaacgatcat
    13501 aagtttttac aaaatagtaa actaaaagga gttgcagcga gactgtgact cctttttggc
    13561 tttatttatt taaaataaaa agaagttttc cggaaatttt tatttaactg tcgtgtacga
    13621 agaaaagttt gctataatgg aaaaaagcgt gcaaagaaag agggagtttc attatgatgc
    13681 tacgtgtaga aaaattgcgc aaaaaaatgc aagaagaaaa tttggattca tttttggtta
    13741 ccagtccgta caatcttcgt tacttaacga actttacagg tacgacagga ttagctgtta
    13801 ttactttgga aaaagcattt tttattactg attttcgtta taccgagcag gcggcagcgc
    13861 aggctcaagg gtttgaaatt attaaaaatg tcggacctat ttttgatgag gtagcaaact
    13921 tagtacgcaa agaagaactg cactcattag cttttgagga aacaacagtc tcatttttag
    13981 aatattcggt tttagaagaa atcatcgatg cggaattagt acctgtttca cagatgatcg
    14041 aagaactcag agaagtgaaa gatgaagaag aaatcgctat tatcgaaaaa gcatgtcata
    14101 ttgcggatat ggcatatgat cacatcctga aaatgatcca accgggcatg actgagattg
    14161 aagtcgcaaa tcaactagac ttttatatgc gttctcttgg cgcttcaggt gtttcttttg
    14221 atacgatcgt tgctagcgga ttgcgttcgg ctatgccgca tggtgtagca agcaaaaaaa
    14281 tcatcgaaca aggtgacttg atcacattgg actttggttg ctattacgaa gggtacgttt
    14341 ccgatatgac acgtacattc gctattggtg atcctgggga aaaattaaaa gaaatttatc
    14401 aaatagtctt agatgctcaa ttagcagtta ttgatgctgc aaaaccagga atgactggtg
    14461 tccaattaga tgcagttgct agagattata tttcaaaaca tggttacggg gaagcatttg
    14521 gacattctac aggacatggg atcggtttag agatccacga aggaccaaac gtttcttctc
    14581 gtgcagaaaa gcaatttgta ccaggaaatg tgatcactga tgaaccaggg atctacttac
    14641 caggaatcgg tggagtacgt attgaggatg acttgttgat cacagaagat ggaaatcgtg
    14701 tgttgactcg ttcaccaaaa gaattgatca tcttatagtt tcatcaatag aagaagtaag
    14761 cgtaaaaatc agtgttcaca gactttgtta tagaaaatca ctcgatttaa tgatacacta
    14821 tagaagaact tttaagctgt aagagataat aaagttgggt gttttctcca attcatagca
    14881 gtttgcagct gatcggcggc agttttctta ccacgtgatc actatgccgc atcatagaga
    14941 attcggatgt atctcgtgct taggcaaagg gaggaataaa taaatggcag atgaaaaaaa
    15001 tttgatttta aacgcaaacc aagatttagg agagatcgtg atcgctcctg aagtcatcga
    15061 agtaattatc gggattgcag cttctaaagt agaaggcgta tacggaatgc gcggaacttt
    15121 tgccagcaat gtcactgaat tattaggacg tgcagctcac ggaaaaggtg tttacttagc
    15181 aaacgaggaa gatggcttga aagtagatct ttactgttat ctagaatatg gtgtttcagt
    15241 tcctaaagta gcaatggaaa tgcaggaacg ggtcagccag caagtccttt tcatgacgga
    15301 tatcgattta tctgaagtca atatccatgt agtagctgtt gttcctgaaa aattagaaca
    15361 accaacaatc gaagaacttt ttgaagacga agaggaagag aatgaataaa gaattatcaa
    15421 gacacgagat tcgggaaatg gcattgcagg ctttatttcc attagacttc aatgcagacc
    15481 taacaaaaga agatgcgatt ttcaacgcaa tcgaattaga ccaccgcgac atgatcaacg
    15541 aagatgaatc agagtttgtt cctgtttatc ttgatacgct agttggcggt gtctgtgcaa
    15601 aaaaagatga attggataaa gtgattgaaa aacatttaaa aaacaattgg cacatcaatc
    15661 ggatctcaaa aatggatttg gtgatcttgc gtatcgcgat ttttgaaatg acttatgtga
    15721 cagatgtacc agcggctgtt gctttaaatg aagcaatcga gttggcaaaa acatttagtg
    15781 atgaccgttc acgtaaattc gtcaacggtg ttttatccaa tgtattgaaa gaattagaag
    15841 ctggggcgta agtcctagct tttttctatg aatcatcata aacctgttat aaatcgggaa
    15901 aattctatgg tagaatgaaa acacaaaaaa tatctgggag tggttagatg gcagaattga
    15961 ttaacggaaa agaattagct gaaaaaatgc aggcagagat tgctgaaaaa atccaaaaat
    16021 tgaaagataa tggtatccat cccggattag tcgttttatt ggtaggggaa aatccagcca
    16081 gccaaatcta tgtaagaaat aaagaacgtg tagcaaaaga aatcggaatc cattcattgg
    16141 tagagcgtta tcctgagacg atttctgaag acgaactact tgcagaaatt gaaaaataca
    16201 atcaagatcc tagttttcat ggtatattgg tccaattacc attgccaaag catatagatg
    16261 aagaaaagat cctgttagct atccgccctg aaaaagacgt agacggtttt catccgatga
    16321 atttaggcag actccttgcg ggtaaaccgg acaagattcc ttgtactcca tacggcatca
    16381 tgaaaatgtt tgaagcttat tctatcgatc cggcgggtaa acgagcagta gttatcggac
    16441 gaagcaatat cgtcggtaaa ccgatggctc agctactctt gatggcagat gcaactgtta
    16501 ctgttgctca ttctaaaacc gaaaatctgg ctgaactggc acaagaagca gatattttag
    16561 tcgtagcaat aggacgtgga cattttgtca caaaagaatt cgtaaaacca ggtgctgtag
    16621 tgatagatgt agggatgaat cgtgatgcta acggtaagct gatcggtgat gtgaaatttg
    16681 atgaagtgga gcctattgca ggctggatca ctcctgtgcc aaagggtgta gggccaatga
    16741 ctatcacgat gctgatgtat caaaccgtaa aatctgccga agcttttgga gcaggtgaat
    16801 agatggcaca agaatattta actgtcacta cattaacgaa gtatttgaaa agaaaatttg
    16861 atgccgatcc ttatttagaa cgcgtatatt taacaggcga aatatcgaat ttccgtttac
    16921 gtgccaatgc ccatcaatat ttcagcttaa aagatgacca tgcaaaaata tctgctatta
    16981 tgttcaaagg agcattccaa aaattacgat tccagccaaa agaaggaatg aaagtccttg
    17041 tcgttgggcg tatttcatta tatgaagcgg gaggttctta tcaaatctat gtagagcaca
    17101 tggagcctga tggaataggt gctttatatc aagaattgga agaacgaaaa gaaaagctga
    17161 tgaaggaagg cctttttcaa gggcctaaaa aaatgctgcc acgtttccca aagcgaatcg
    17221 cggtccttac cagtccaagc ggagcggtta tccgcgatat catcacgacg gtgaaaagaa
    17281 gatatccaat tgtccaattg gttttgttcc caacagttgt acaaggaaac caagcagcgg
    17341 acaatgttgt caggaatatc cagagagtag aagagatggg caatttcgat acaatgatca
    17401 tcggtcgtgg gggtggttcg attgaggatt tatggccgtt taatgaagag cgggttgctc
    17461 gggcgattta tcaagctaat acgccggtga tttcatctgt aggacatgaa acagatacca
    17521 cgatcgctga tttagtagca gatgttcggg ctgccactcc aacagcagct gcagaattag
    17581 ctgttcctgt tttgtcagaa gagttgatgc gcattcaaga acggcaagct agattgcagc
    17641 aggcgtttgt acgccagatc cagcgcaaac aagaacggtt cgagcgtgct cgacgttcct
    17701 atgtatttcg gcagcctgaa agattgtatg aaggtcagac catcaaatta gaccagttga
    17761 aacagcgatt atatcaagct acacagcaaa tttatcatgc gaaagaaaaa caaacgatcg
    17821 cattaagcca ccaattggaa caagtggctc cgctttatcg agttaaagca gctcgccaag
    17881 aaactgctta tctagaaaaa cgcttagaag agaaaatcca acaatatatg aaacaacagc
    17941 agcagcgttt tgctcaagct gttcaatctc ttgatttgtt gagtccattg aaaatcatgg
    18001 gaagaggcta cagctatacg acgttaaatg atcaagtagt aaaaagtgtc accgagattc
    18061 atcaagggga tttgctgacg atccattatc aagatggaat cgttaaagga caagtacaag
    18121 agatagacaa aggagaataa aaatatggaa aatccaactt ttgaagagtc actgcaagaa
    18181 ttagaaaaaa ttgtcatgca gttagaacaa ggagacgttc cattagaatc agctctagat
    18241 tcgtttaaac gaggaatgga attaagcaaa cattgccaag atacattagc taaagcagaa
    18301 gagaccttga cgaaaatgat gacagaatca aatgaagaag ttgattttga cggaaacgag
    18361 gaaagctgag atgagattaa ctgacttttc agctattcat ctacctgctg tagaaaaaga
    18421 aatcttgtct tttttagatg agcatacaac ggatagagga ctctatgatg cgatgagtta
    18481 ttcagtgaaa gcaggcggga aaagaatacg tcccttatta cttttaacgg ccgttgcttc
    18541 ctttgatgag cctattgatg ttcccgtcta tcaagtagca gctgctttag aaatggtgca
    18601 tacgtattcc ttgatccacg atgatttgcc tgcgatggac aatgatgatc ttcgtagagg
    18661 aaaacctacc aatcataaag tattcggcga agcattagct atattggccg gagatggttt
    18721 gcttacagga gcattccagc taatcagtat ggcacactta ggtaattctc ctaagcttct
    18781 gctgcttcag caattagctg tttgtgcggg atcacaaggt atggttgctg ggcaagctgc
    18841 agatatcgag ggcgaatcga aaaagctttc tttagaagaa ttagcattta ttcatgaaag
    18901 aaagacaggg catttgattc gttatgcctt gctggctggt ggcatattag cgaagcaacc
    18961 tgaagaaatc ttattgctac ttcaacgatt agccgaacat ttaggtttag cttttcagat
    19021 aagagatgat ctactcgatg tgattggtac aacgaaaaca ttagggaaaa cagctggtaa
    19081 agatgaacgt atggaaaaaa atacttaccc gcgtttatta ggcctagaaa agacacgtga
    19141 ggcgttggag attgagctgt tatccgccaa caagatcata gacaaattag aagaggacgt
    19201 tttttctttt gatggcgggc tgatgcgcca gatgatcaag caatttgatg tggagaaatg
    19261 agggaaagaa tggaaaaaga acgcgtagat gtattagcgg tcaaacaagg attatttgaa
    19321 acaagagaac aagccaaacg ttcagtgatg gctggtttga tttacaacga aaaaaacgag
    19381 cggtttgata aaccgggaga aaaaattcca gtgagtagtg aattaagagt caaaggtaaa
    19441 aaacttcctt atgtttctcg agggggatta aaattagaaa aagcactcaa agcatttgat
    19501 ctatctgttg aaggaaaaac attgttagat attggtgcat ccactggcgg atttactgat
    19561 gcagctttgc aaaatggcgc aaaaatgagt tatgctttag acgttggcta caatcagcta
    19621 gcttggaaaa taagacaaga tcctagagtc gtcgtaatgg aaagagtgaa ttttcgttat
    19681 gcaaaacccg aagactttac cttaggaatt ccagaagttg ctgttatcga tgtttctttt
    19741 atttcactaa aactgatgct gcctccatta catgctattt taaaagaaga gggagaagtg
    19801 attgctttaa tcaaacccca attcgaagca ggcagagaag ctgtagggaa aaacggaatt
    19861 gttcgtgacc ctcagaccca tcaaaaagtt ttagaagaca ttttgtcatt tgcggcacaa
    19921 ggtggatatg atgtgctgga attaagttat tctccgatca cggggggaga aggaaatatc
    19981 gagttcttgg ctcacttgag gaaagtaccc gaaagcggaa caatcaattc agctattaat
    20041 atggcagaag tggtttcaaa tgcacacgaa cagtttgacc ataaataaat tgtaactgtg
    20101 aaaatcaagt gagaagtatg cggattcttc tttattatac caaggaaata agctatcatt
    20161 gattaagaag aatatgctaa ggagcgaaga cgtaatgaga aaaaaagatc ggcatcgttt
    20221 gattactcga ttgttgagtg agcaagatat ccgaaagcag gaagaatttg ttgaaatcct
    20281 gaaaagcaaa ggaatatctg ttactcaagc tacgatttcc agagatataa aggaactgaa
    20341 actgatcaag gtaccggctt cagatggcgg ctaccgttat agcataccag ctgaaacaaa
    20401 cgaagatgta aatactaaat tggaaaaact attaaaagac gctttcgtgg cagttgacca
    20461 aatggaaaaa tttgttattt tgaaaacatt gccgggaaat gcttcggcag cagcaaattt
    20521 gattgataaa cgttacaaga aagaattatt ttcgattatc aatgatgacg ataatgtttt
    20581 gatgattaca cgaactgaag accaggcagt tttattgaag aaagacttcc tgcattacct
    20641 ttaagttgag gagcgaatgt gatgctacaa gaaatcagta ttactaactt tgcgattatt
    20701 ccggaattgc ggctttcttt tcacgaagga atgaccgcgc tgaccggaga gactggtgcg
    20761 ggtaaatcca ttattatcga tgctttaggt ctgttagcag gaggaagagg ttcaagcgat
    20821 tatatacgtc aaggagcaga aaaatgtgtg cttgaagggt tgtttgaact accaaaacaa
    20881 gaaggattct ctgaactgat ggttgaatta gggatcgaaa cagatgagga caatctgatt
    20941 gttcgacggg atatgtcctt aactggaaaa aatgtttgtc gagtgaatgg tcatattatc
    21001 acattagcta atctgagaag aatcggcagt tatttagtag atatccaagg acaaaacgaa
    21061 catcaggaac tgctccagcc tgaatcacat cttgctttat tggatcgttt tggagatgta
    21121 gctttccaac agaagaaaaa gagttatcag caagaatata tctcttatcg tgagctggaa
    21181 aaaagggtac gaaaagtgca gcaaaatgaa aaatcctatg ttcagagaat agatatgctc
    21241 catttccagc aagaagaaat agctgctgct gatttgcaag tcggagaaga agagaaatta
    21301 aaagaagaaa gagaaaaact tagcaattat caaaaaattg ctgatggatt agctgcaggt
    21361 tacggagcac tgacgaataa tgaacaaaac agtgtagatg gtgttggctt ggccgtttca
    21421 gaactgcaag ggatcgccca tctagatgtg gagtatgaag caatttatga aaatatccaa
    21481 agtgcttact atttgctgca agatgcaatc ggcgatatga gtcgtcagat tgatttattg
    21541 gagttagatg agagtcgttt agaagaagtt acgcaacgtt tagaattaat ccgacagttg
    21601 aaacgtaaat acggcgaatc cattgagtca atattggctt actatgacga aatcaccgaa
    21661 gagctagctt cttctgattt ttcagaaggt caattggaca aaatgaaaga ggaactagag
    21721 caaaaagagc ttctacttca gcaacaagcc gcagatcttc atgaagcgcg taaaaaaatc
    21781 gcaaaagaat tggaaaaatc cattctgcat gaattgaaaa gtctgtatat ggaaaacact
    21841 gagtttgagg tacgtttctt aaaagaagaa aatagacaat tgaatcatga tggatttgac
    21901 caaatcgaat tttacattac gaccaatcca ggagagccac tgaagccttt ggtgaaagtc
    21961 gcttctggtg gcgaactgtc tcggatgttg cttgctttga aaactatttt ttcttctgag
    22021 caaggtgtga caagtattat ttttgatgaa gtggatacag gtgtgagcgg aagagttgca
    22081 caggccatcg cagataaaat ttctcaaata tcgaaatact ctcaagtttt gtgcatcact
    22141 catctgcctc aagttgctgc agtcgccgac tatcaatatt atattgtgaa agcagtgatt
    22201 gatggacgta cgcaaacttc tgtttcagag ttgaaaacaa aagaaagaga agaagaaatt
    22261 gcacgtatgc tggctggaag tgagattaca aaactcacac tcgaacatgc aaaagaacta
    22321 ttgaaactcg caaaaaggac ctgagccacc tcgcagtggt tcaggtcctt ttcagcagct
    22381 gatagctata ttttattttt acatgacact cattacagca gtcaatacaa cagatccaat
    22441 cgttaaaatc agcattgtcc aaatgacaaa ttttgttact ttagcaaaag tacttgtttg
    22501 ttttttttca cgcattgttt tcaacctcgt cattaattag ttgattcaag tgtacaatct
    22561 tttttccatt tccacaagag ggctttttta taaaaacttc ccttttttta aataataggt
    22621 agttacgata caaataaata atgttaatag aactagccat ataaaggcat catccctttc
    22681 agcaataggt aacttaatgt tcattccata gatactgccg acgattgtcg gtatagttaa
    22741 aataatcgtt aaagaagtta aaattttcat aatattattc agattattcg atacgattgc
    22801 tgaaaaagtc tgattcattt gttctactag ttttaattga atgcgagaag tagtcattgc
    22861 ctgctctgtt tccaccaata catcacgtaa atgatttttc aaagcatgat tttcttggaa
    22921 aagtttattt tgcgctagtt ctttcaatgc atctaaatta gcagaagtcg cttctctgaa
    22981 ataaacgagg cttttttgga tatccatgat ttgataaagt tgtttgtttt cagtggatac
    23041 ttgaagtttg gcttccaaag tatcgcattg agattgaagc gtagctaaat aactgttgta
    23101 agagagtgtg acctgccaaa tgaattgaaa gaagatattg agattaggcg aaatatcact
    23161 ttttggaaag gtctggctaa agactttctt taaaaacagt ggttcatgat tcgtaattgt
    23221 gataagcttc tttttgggcg tcaaaataat agagagcggg tatgtatcca cttgaaaata
    23281 accgctagga cttgttttag caaaaggata ttgtaacaat aataacgctg gctttgtcaa
    23341 ctttgattga ttgaattctt ctgtacggga attttctgca tcgtccaata tggaagtgat
    23401 gtaatctgac ggaatttcaa attgatgtgt cagctgatga atctcttctt gattaggacg
    23461 ttctacacaa taccagatcg tttggtcagt ttcctttccg gtattacgca gtttctcatt
    23521 atctaatgta aaatatttga tcatgatttt cctcctgttg cctgtttctt ttattataac
    23581 ggatagggaa ttggaataca tggaatgtgt ttgtaagata tagaatataa ctgcataaat
    23641 ttaaataaaa tgaaacagtt gtgtaaagta tatacagtat acttgaagca acgataaaaa
    23701 aggagtttac gttcatgggc acattcacac cacttatctt gttttggtat atttacccgg
    23761 taatcgtttt gtttggttgc caatttcttg taaaattatt ttcgttaaat cgtcgtttta
    23821 aaataaaagc ccctgatttg gcgattcctt ttttgtgggg cggtatgcat gcattgtcta
    23881 gaaatacagg gacaatttct atcttaccgt ttttctttat ttctgttttg ctaatgggaa
    23941 tttgtattgc tgttttccaa gcatattatt atgaagaaat tatttatccg cgttatttca
    24001 agatgacttg gcgtttggtt tttctgttga caatcgtctt gtacgctgtc ttgatcattg
    24061 tgaacatact ttcttaccta tgaaaaaaat agaaaagctg taaaaagtct tttaaaaggc
    24121 tttttgcagc ttgattttta aaaaaatgtt tatgaataaa aaaagttgtt cccggatttc
    24181 ccagaaaagg tggtagaaag tgggggattg tggtagacta attttagtta gtggaggaat
    24241 ggggggcttt caatgttgat gggtgaattc caacataata ttgatgcaaa aggacgttta
    24301 atcgtgccct caaaacttcg tgaagaactt ggcgaaaagt ttagtggagg aatggggggc
    24361 tttcaatgtt gatgggtgaa ttccaacata atattgatgc aaaaggacgt ttaatcgtgc
    24421 cctcaaaact tcgtgaagaa cttggcgaaa agtttgttct gacacgcgga ttggatggct
    24481 gtcttttcgg ctatccgatg agcgaatggg aaaatctgga agctaaactg aacgaaatgc
    24541 cgttggctaa aaaagacgca cgtacttttg tgcgattctt ctattcggct gccactgaat
    24601 gcgaacttga taaacaagga cgaatcaata tacccagcac gttaagaaat tatgcagctt
    24661 tgacaaaagg ttgcgtagtc atcggtgtat ctaaccgaat tgaaatttgg gatgagacac
    24721 gatggcaaga tttttctgca gcagcagaag agaactttga cgaaattgct gaaagtatga
    24781 ttgatttcgg actataagaa taggaagagg ctgactaatg actgaaacat ttcaacacta
    24841 tacagtcatg ttgaaagaaa cggtagatgg attgaacatc aaaccggatg gcgtttatgt
    24901 agactgtaca ctcggcggtg ccggacatag tgaatattta ttaagccaac taagttctaa
    24961 agggcatctg tatgcgtttg accaagatca aaaagcaatc gatcacgcca agctgcgttt
    25021 agcttcttat gtcgaacaag gtatggtcac atttatcaaa gcgaacttca gagaattaga
    25081 agaacgcttg agtgagcatg tagataaggt agacggcatt ttgtatgatc taggggtttc
    25141 ctctcctcag ctagatgaag cagaacgtgg atttagttat catcaagatg caccgttgga
    25201 tatgcgcatg gatcagtcag ctcctttatc cgcttatcac gtaattaatg aatattccta
    25261 ccatgaatta gtaaagattt tcttccgcta tggcgaggag aaattttcca aacaaattgc
    25321 tcgtgaaatc gaacgagtac gaaaaacaaa accaatccaa acaactggtg agcttgtcga
    25381 aatcattaaa caagtcatcc cagctcccgc aagaagaaaa ggcgggcatc ctgcaaaaag
    25441 aatttttcaa gctgtgcgga ttgcagtaaa tgacgaactc ggtgccgaag aggcctcttt
    25501 agaacaggca atccggttat tgaaagtcaa tggaagaatc agcgtaatca cattccattc
    25561 attggaagac agaattgtca aaagtatgtt taaagaatat agtacggtac aagatctacc
    25621 accgggactt cctgtcatac ctgacgaatt ccaaccagaa ctgaaagtca ttactcgtaa
    25681 accaatcctt ccttcagagg aagagctaac agaaaacaat cgatcacgca gtgcaaagtt
    25741 acgtattgca gaaaaaataa gaatgaaaga ggagtagaat gatggcagaa ttaaagaaaa
    25801 tgcaagatta cgaatatgat ttacctgaat tggtggatca gccggaacaa caaatgcctg
    25861 attcttcttc gaatcaagaa atcattcttc cacaatcacc ggcaaaacga ttaaagcaga
    25921 tttctgcttt ggaaaaaatt gctgtggcaa gcgtggcgat tgctattgtt gctttatgtg
    25981 ttttaacagt tatgctgcgc acaaatatca gcggagctga aaaagaaatc acgtcaatac
    26041 aaacagaaat caatgataaa aagcaagaaa aaacgagttt agaacaagaa aaaaacgaac
    26101 tttctcgtac tgaacggatt aaacaaatcg cagaagaaaa aggattgtct attaatgacg
    26161 acaatttaag gaaagtgaag taaatgagtc ttaaaaataa aattcggcgg tttatggaaa
    26221 agaaaaatct aaatccgatg aacaatcgca aaaaagtagg tatcatttta tttgctacga
    26281 gtattggatt gttcttttta tttgccgtcc gtttctcgta tatcgttatt ggaggtcatg
    26341 ttgctggtac ctcattagct gaaaaaacaa aacagttgta ccaaggaagt gaagtagtaa
    26401 aagcaaaaag aggaacgatt tatgatcgta acggtgtggc gctcgcagaa gatgcctcct
    26461 cttattcaat taaagcgata ctctcaaaaa catatacctc tggggataaa aaactgtatg
    26521 ttgaagaaaa gaattttgat aaaatagcag aaatcctgca taagaatctt tctatagaca
    26581 aaaaagatgc actgaatatt ttagaggacg gcgcaaaaaa agagctgtat caagttgaat
    26641 ttggtagcta tgggaaaaat atctcccaag aaaccaagca aaacatcgaa gcggacatga
    26701 aaaaagaagg tgtcgctggt ctttattttg tcgaccatca ggctcggatg tatccaaatg
    26761 gcgtcttttc ttctcatttt attggatacg cagttccaga caaagatgag aatggcttag
    26821 ttggtaaact aggtttggag tctgcttaca atgatatctt gagcggtaaa gatggtaaga
    26881 ttatttatca aaaagataat tttcaaaatc ctctgccagg tacagtggca gaagaagaaa
    26941 aagctgtcga cggacaagat atctacacga ctttagacag cagactacaa agttatttag
    27001 aaacactgat ggatcaagta aatgaagaat atcagccaga agaattgaca gcagtcctga
    27061 tgaaagcaaa aaccggagaa atcttggcaa tgggacaacg acctactttc aatcctgaaa
    27121 caatggaagg attgactgga aaagatgcta tctggcgtaa tttcttggta caagatagtt
    27181 atgaaccagg atcaaccatg aaagttttca ctactgcagc tgctatcgaa gaaggagaat
    27241 tcaacgagaa tgaaaccttc caatctggaa aaatccaagt agaagatgct acgatcaatg
    27301 accatgactt tggtgaaaaa ggtgtgctga ccatgcgtca agctctttca tggtcaagta
    27361 acgtcgggat ggtcatactg gaacagcgat taggcggacg atggtataac tatttacaaa
    27421 aactgggatt tggacaaagt actcattctg gattagatga tgaagtaaac ggtgctttac
    27481 ctacattaaa tattgtcgac cgagcaatga gtgcttatgg acaagcggta ggtgtgacga
    27541 atttccaaat gatgaaagct ttcacttcta ttgcgaataa cggtacaatg attcaacctc
    27601 gatatatcag caaagtggtg gatccccaaa ctggagaaga gcgaacaact caaacagaag
    27661 ttctagggca acctttttca aaagaaacaa cagaaaaagt ccgtgaatat atgagagatg
    27721 ttgtcgaaag cgaaaactac ggtagtgctt atggtgtata tagtgtacct ggatacaatg
    27781 tatcggcaaa aaacggaaca gcccaaatcg cttctgatac agggggatat caaacaggag
    27841 atacagccta cctgtattcg attgtggaga tggttccttc tgaagatcca gactatgtct
    27901 tgtatctaac aatgaaacac cctaagacat atgatcgaat ggcattggct aaaatcgcaa
    27961 atccattgat gaaacgggca atggatttta aagaaactga agaagattca gatacagaaa
    28021 caaaaacaga aaaagtatcc gtagccgatt atcgtaatct tgaagcggac gttgcagcag
    28081 ctgatgctca aaaaagtgga ctgcaacctg tagtgatcgg taatggtaaa aaagtacaaa
    28141 aacaatctac tgccaacggt gatcagttga tctctggaga aaaactgatc ctatatactg
    28201 gtggtgataa gctgatgcct gacgtaacag gatggtcaaa agcagatatc atgaagctgg
    28261 ggaaaatcct cggtattgaa gttagttttg atggagatgg ttattgtgtc aaacaagaac
    28321 ttgctccata tgaaaaaatc actgaagaca aacttagttt tactttagaa gaataagaag
    28381 agaaatagga gagaaaagag catggaatgg acacaagcat tgattcccat tgtcagcagt
    28441 tgtgcaatga caattgccgc aatgccgcta tttattggtt attttcaaat gaaaaagcaa
    28501 ggtcaggcga ttcgtgagga gggaccaaag tggcacaata gcaaagccgg aactcctaca
    28561 atgggcggct tagtgtttct gatcggctct attttgacag gaatttgggt tggagcttgg
    28621 caaaaacagc taactcctac tcttttcatc ttgctttttg tattagcgtt atacggagta
    28681 atcggctttc ttgatgattt tataaaaata ttcaagaaaa gaaacatggg actgaactcg
    28741 aagcagaaat tgattggaca gattctaggg ggaatcatct tttatcttgt ttaccgttct
    28801 gaaggttatc caggtgtatt gaatttcttc ggactagatc tacctttagg aatcatttat
    28861 ggtattttcg ctctcttctg gcttgttgga ttttcaaatg cagtaaatct gacagatggc
    28921 atcgatggat tagttgctgg cttaggaagt atctcttttg cagcttacgg tctaattgct
    28981 tggcatcagc atcagtatga tgtattaatc atttgcttaa gtgttttagg aggtttactg
    29041 ggtttcttcg tctacaacag aaaacctgcc aagattttta tgggagatgt cggttcatta
    29101 gcgttgggcg gacttttagc ggcaatttct ataatgctaa accaagaatg gacattgctt
    29161 ttgattggtt tgatctatgt tatggaaaca gcaagtgtga tgcttcaagt tacttccttt
    29221 aagcttacag gaaaacgaat ttttaaaatg tctccaattc atcatcactt tgaaatgtgc
    29281 ggatggtcgg agtggaagat tgatacagtt ttttggttga tcagcattgt gacttcgttg
    29341 atcacattat ggatcgtttg gtaggagatg acgggaaatg aaaaaaatat caacatatga
    29401 aaataagaaa gtgcttgtct taggactcgc aaaaagcgga gtaagtgcgg ccaaattatt
    29461 acatgaattg ggcgcacttg tcaccgttaa tgatggaaaa ccttttgacg aaaatccgga
    29521 agcacaagag ctgctttctt tagggataaa agtaataaca ggtagtcatc caatcgaatt
    29581 gttggatgaa gaattttcat tgatggtcaa aaatccaggg attccctaca gtcatccttt
    29641 agtagcaaaa gcccaagaaa tgggtattcc ggtaattacg gaagttgagc tagcttacga
    29701 agtagcggaa tgcccaatta ttgggattac aggaacgaac ggaaaaacaa cgacaacaac
    29761 gatgacagga ctactactga atgcaggagc agatcaagga attgcacgtt tagcgggtaa
    29821 tattgggtat ccagcaagtg gtgtagcgca agaagcaaaa tcagaagata aaattgtgat
    29881 ggaattgtct agcttccaac tcatgggaat cacagatttc cgccctcata tagcggtgat
    29941 taccaatatc tatgaagcac atattgatta tcatggtaca agaaaagaat atgtcaaagc
    30001 taaatggaac cttcaaaaaa acatgacaga aaaagattat ctgatcctta actggaatca
    30061 gtcagaacta caagaattag cgcaaagaac aaaagcacgc gtactgcctt tctcgacaaa
    30121 agaagtgttg gaagacggtg tgtatgctga cgattactca atatactata aaaaagaaaa
    30181 aataatggaa atatctgaac ttggcgtacc aggtaagcat aatgtagaaa atgcactagc
    30241 tgctatttct gtagcaaaat tgtatggtat atcaaatgaa gctatacgag aaacgttgca
    30301 ttttttccat ggcgtaccac atcgtacgca atatgtagga gaaatccaag gcagaaaatt
    30361 ttataatgat tcaaaagcaa ccaatatttt agcgactaaa atggctctaa gtggatttga
    30421 aacgtctaag gtcgtgttat tggctggcgg attagacaga ggaaatacat ttgatgaatt
    30481 aattccttca ttaaaaggaa tcaaagcaat ggtcgtattt ggccaaacaa aagaaaagtt
    30541 gatggatgct ggaaaaaaag caggcatcga gacaatagta acagccgatt ctgtagaaca
    30601 agcagttcct ttagcattag aaaacagtac agatggtgat gtagttttat tatcaccagc
    30661 gaatgccagc tgggatcaat acccaaattt tgaaactaga ggaaacagat tcatggaagc
    30721 tgtgaaccga ttaaaataga aaaagtagaa gcgagcgata aaaaatgaaa attttggtta
    30781 caggcggagg aacaggggga catatttatc cagctcttgc ctttgtcaat tatgtaaaga
    30841 ctcaagaacc gaatgctgaa ttcatgtatg tgggagcaaa acgaggtcta gaaaacaaaa
    30901 tcgtccctga tacaggaatg cctttccata ccttggagat tcaaggattt aaacgaaaaa
    30961 tttccatgca taacatcaag acggtccgat tgtttcttaa gagtatccgt caagcaaaaa
    31021 aaatcctcag tgattttcaa ccagatatcg ttatcggtac tggaggatat gtttccggag
    31081 cggtagtgta cgctgcagcc aaaatgggta ttccaacagt tatccatgaa caaaacagtg
    31141 taccaggtgt taccaataaa tttttaagcc gatatgtgga tcggatcgct ctttcattta
    31201 aagatgcggc ttcctttttt cctgaaaaca aagcagttct tgtaggtaat cctcgagcac
    31261 aagaagtagc agatactaaa aaatcagata ttttgaaaac atttgatttg gatccagaaa
    31321 agaaaacagt cttgattttt ggcggtagtc aaggagcact taaaatcaat caagcagtat
    31381 cagttttttt aagtgaatat attccttctt cttaccaagt tttgtatgct tcaggagata
    31441 gatattatga agagatccta aataaaataa atcctgaaat acaaaatatt agtgtccagc
    31501 cttatatcaa aaatatggca gaggtcatgg cttccagtga tctgctggtg gggcgtgctg
    31561 gtgcaacttc gatcgcagaa ttcacagctc tggggttacc ggcagtgttg gttcccagcc
    31621 catatgttac gaatgatcat cagactaaaa atgcaatgag tcttgttcac gcaggagcag
    31681 caaaaatgat tgcagataat gaactaacag gtgaatcact aagtcagaca gtcaacgaaa
    31741 tcatgggtga tgaagaattg caaaagcaaa tgtgccgagc ttctaaagaa caaggaatcc
    31801 ctgatgcttc aaaacgtctt tatgacttag taaaacaaat aatcaaaaaa taaaggcggt
    31861 ggtcttagtg agtaagaaaa aagaaccaaa aaaattgtct catccgtctt ctgatgaaaa
    31921 caatttgact ccttggcaaa aggctaaccg aaaatatctg gcagaacaag agatgaaaaa
    31981 ccaagagcca aaaaaagaag acataacatt agatacaccc ctttcagatt ctgaagaaga
    32041 aaaaacggtt caagaggaac agcaagaaga aagccagtcc gagaaaaaaa tcgaattttt
    32101 tgaagagatc aagcaaaaga aaaacggtgg accatataac ggttcttttt tgaaccggct
    32161 gcccaattta aaaagtcaac ggcacaaagt actatttcgt cggttagctt tgatcatcac
    32221 tgtattgggt ataccgctga tttttttgat ctattatgtt tctccttata gcaaattaca
    32281 agcagttgca gtatcaggca ataagacagt caacagccaa gaaatcatct ctgatacaaa
    32341 attgtcatta ggagaaaatg tttggggaca gtattttcat cgatctactt atatcgatcg
    32401 ccttaaaaaa gcacagccaa gaatcgaaac agcaaatata catttcaaag ggatgaatga
    32461 attcgatttg gatgtaacag aatataaaga gattgctttg atcgcaaaaa acaatcaata
    32521 ctacccagtg attgaaaatg gaacagtatt agatgaaaaa gtagcaaatc ctacaaaaaa
    32581 tctccctatc ttggaagagt tcaaagactc tgcaaaaatc aaagaattaa caaagcaata
    32641 caatcagtta tcatcagaat tacaaaaagc aatttctgaa atcaagtaca cgccaagagc
    32701 aagcaataaa aatctgatcc aattgaatat gaatgacggc aatcaagtta ttgtaaatat
    32761 caataatctt gcgaaccaaa tgaaatatta ttcgcaagta gccaaagata tggatgaaaa
    32821 aggtgtgatc gacatggaag ttggtatctt ctcgcatcct tacggtagca ctcaagaaac
    32881 ggaagaatcg ggccaatcgg aagaaacaac tgaaagtgaa gaaaatgcgg atcaaacaaa
    32941 cgaatcaagt gaagaggcag aaacgcaaaa agcacaaaca cctggatcgt ctagcacaaa
    33001 cggatcatct gattcaaatg attcaagtga aacaagtcaa atagaagata atccaagtac
    33061 aactccgtca agtttaaatg aaaattgata aaacaacttt aaaaagtaag aatgtacttt
    33121 ctaaaaacca gtaatttatg gtagaattgg aagcagtgaa tatatctatt aataagtata
    33181 ttttaaaacg gacgctagtt gttcgcttgt atatggaata gtaagtagga gggaatccca
    33241 ttcatggcaa aaacaggaat gtatgtaggc cttgatattg gaacgacatc tgttaaagtt
    33301 gtcgtggctg aatatatcga cagccaaatg aatattattg gagtaggaaa cgcaaaatca
    33361 gaagggatta accgaggcat tatcgttgat atcgataaaa cggtccaagc gatacaacga
    33421 gcagtacgac aagcagaaga aaaagcaggt atccagatca aaggcgtaag tgtcggtttg
    33481 ccagcaaaca tgcttgaagt agaaaattgc cagggaatga ttgcagttaa cggagattca
    33541 aaggaaatca ccgatgaaga tgtcagaaat gtggcttcag ctgcccttgt tcgttcaata
    33601 cctcctgaac gtcaaatcgt gtcaatattg ccacaagatt ttactgtcga tgggttcgag
    33661 ggaatcaaag atcctcgtgg aatgatcggc gtaagattag aaatgtacgg tctgttgttt
    33721 acaggaccta aaacgatcat ccataacata cgtaaatgcg tggaaaatgc cggattaatc
    33781 gtcaacgaaa tggtgattac tcctttagcg ctgactgaat caattctttc tgacggagaa
    33841 aaagattttg gtacgatcgt cattgatatg ggtggcggcc aaacaacaac ggctgttatg
    33901 catgataagc aattgaagtt taccaatctt gaccaagaag gtggcgaatt tgtcactaaa
    33961 gatatctcta ttgtattaaa tacgtcattt aacaatgcag aagcgttgaa aataaactac
    34021 ggggatgcat acccagaacg gacatcacca gatgaagaat ttccagtaga tgtgattggt
    34081 cagtcagaac cagtgaaagt cgatgaacgc tacttgtcag aaattatttc agctcgaatg
    34141 gaacagattt tcaacaaagc aaaagatgca ttggaccaaa tcgaagcatt agagttgcca
    34201 ggtggcgtcg tattgacagg cggagctgct agtcttcctg gtgttgtgga cttggcacaa
    34261 gaaatcttcg gcgtaaatgt aaaattgtat gttccaaatc acatggggct gagaaacccg
    34321 gtatttacaa atgtaataag tatcgtggat tattcagcaa acttgagtga agtgtatcaa
    34381 ttggcaaaaa ttgctgttac gggtgaaacc tcagcagctc gtcaaatggt agttgaacaa
    34441 gaaacaacaa acacatatga gagctatgaa gcacctgaag aaacaatcta cgatgagccg
    34501 gaaccaaaag agtccggaga aaatgtaaaa aataaaatca agggtttctt tacaaatatt
    34561 tttgattaat tcatcctggg aggaataaga tatggaattc tcaattgata ataatatcaa
    34621 tgacggcgca gtaatcaaag tcatcggagt cggtggtggc ggtggaaatg ctgtaaaccg
    34681 aatgattgaa gaaaacgtta aaggtgttga attcattaca gccaacacag acgtgcaagc
    34741 attaaaaaat tcaaaagctg aaacagttat ccaattaggc cccaaatata ctcgtggttt
    34801 aggtgctggt tctcagccag aagttggaca aaaagcggca gaagaaagtg aacaatcctt
    34861 acgcgaagct ttagacggtg cagatatgat tttcatcact gcaggaatgg gtggaggaac
    34921 cggtacaggt gctgcaccaa tcgttgcagg tatcgcgaaa gaattgggcg cattgactgt
    34981 tggtgtcgtg acacgtccat tcacttttga aggaccgaaa cgtggccgtt ttgctgcaga
    35041 aggtatcgct cgtttgaaag aaaatgtgga tacattatta attatctcaa ataaccgttt
    35101 attagaagtt gtagataaaa agacaccgat gcttgaagct ttccgtgaag cagataatgt
    35161 tttacgacaa ggtgtacaag gtatctctga tttgatcaca gcgccaggtt acgttaacct
    35221 tgactttgct gacgtgaaaa cagtgatgga aaaccaaggt acagcattga tggggatcgg
    35281 tgtggctagt ggagaagatc gcgtagtaga agcaacgaaa aaagcaatct cctctccatt
    35341 gcttgaaaca tctattgatg gagctgaaca agtacttctt aacatcacag gcggtttgga
    35401 tatgacattg ttcgaagcac aagatgcttc agatatcgta gcaaatgcag caactggcga
    35461 tgtaaatatc attctaggta catctatcaa tgaagaaatg ggcgatgaaa tccgtgtgac
    35521 ggtcatcgca acaggtatcg atgaatcaaa aaaggaacgc aagtctagcc gaccagctag
    35581 acaagcacaa atgcaatcac ctgctcaaaa gactgtatta gatatggatc aagctaaacc
    35641 aacttcttca gaagaagaaa acagttttgg cgattgggat atcagacgtg aacaaaatgt
    35701 tcgtcctaga gttgatgatt ccaattttga caatatcgaa aaaaaagaat ttgatacatt
    35761 caaccgcgaa gaaacaaaat ctcatggcga tgatgaatta aatacgccac cgtttttccg
    35821 tcgtaaaaaa taaaggagag gaagagcaca tgattgctga caacttgcag aaaatcaagc
    35881 aagaaattca ggattcgtgt gctcttgttc atcgtgatcc gaaagaagtt acattagtag
    35941 ctgtaacaaa atctgttgaa tccgctcaag ctgaagaatt ggttagagct ggcgttatga
    36001 attgtgcaga aaaccgagta gataaattat tagaaaagaa acaagattta tttgattttc
    36061 ctgaaataaa gtggcattta attggtaatt tacagcgcag aaaggtaaaa ttgattgtaa
    36121 atgaaattga ttactttcat gcgttggaca gtttaagatt agcagaagaa atccagaagc
    36181 ggttagagaa agaattatcc tgttttattg aagtaaatgt ttctggtgag gaatcaaaac
    36241 atggaatttc accagatgag cttttaccgt ttgtggaatc tttagctgcc tgcacaaaaa
    36301 tcaaggttgt tgggttgatg actatggcgc ctaaagaagc ttctgatgag caaatccata
    36361 aagtgtttgg tacgctgaaa gaattgcgtg atcttgttca agcaaaaggt tatgcccatg
    36421 caccatgtag tgaattaagt atggggatga gtcaagactt tcaaattgcg attgaagaag
    36481 gtgcaacatt tatccgtgta ggaacagcgc tattcaaaga cgaataaagg aggaacaaaa
    36541 atgccgcttt taaacagagg agcaattgct aattttttcg gattagctga tgaggaagag
    36601 tacgaatacc aagattatcc tgaaacaaca aaaaaggctg tacagccaac taaaaaaaat
    36661 acaacacaga atcaatcaac tcattctgta tctgctgcga atactcatga aaaacaaaaa
    36721 acggcgactt cacgtcctgt aacgcatgaa agtattcagc cggctgaaaa gaaaaaagta
    36781 attgaactac atcaatcacc tacaaaaact gtagcgaaag aaagaacagc ttcaagaacc
    36841 tctacacaac ctaaagcaaa tcttgctgga aaaatcactg taatggaacc aagagcttat
    36901 tctgaagcta tggcgattgc taaacgaatc attgctggag aagcaacctt agtgaatttt
    36961 catttagtag aagaaaaaca agcgagaaga atcgttgatt ttctaacagg aacagtttat
    37021 gcactagatg gagatatcaa acgtgttgga gatgagattt ttctttgtac accgactggg
    37081 atggagattg acagttcaac cgctcaatct tttgcagatg cgaatttgtt tgatctgtaa
    37141 gaggaggaac tacgatttat actattgcac tattacttca tttacttcaa cgggcagcct
    37201 atttctatag cattatcttg atggtatatg cattgctctc ttggtttcca ggcgggtatc
    37261 aatcagggct aggacgatta ttagctaaga tctgcgaacc gtatatcagt ttgttcgatc
    37321 gtctgccttt gaggattgga cccttggatt tcacaatttt aggggcaatc ctgatcttga
    37381 acttagccgt acaaggttta gcacagatcg ttatatggat aactgtccat ttagtttatt
    37441 gattgaagca acaaaatctt tattctcaga atagatagga ggggaaaatt ttggatgcaa
    37501 atgtatacca gcactttcgg gcagatgaac gtacatttat cgattcggta ggcgattgga
    37561 ttgaacaggt tcaaggacaa tatgcaccct atctgaccga atttcttgac ccaagacaat
    37621 cctatatttt agaaacactg attcgacaaa gcagtgattt gcgtttcatg ttttatggcg
    37681 gatatgaaca ggcagaaaga aaaagatgtt tgattttccc cgattattac gaacctgtag
    37741 aggaagattt tgaaacagca ttgtatgaaa tccattatcc tagcaaattt gccagcctaa
    37801 gtcatgggaa aatccttgga actttagtgg gaacggggat caaacgctct tactttggcg
    37861 atatcatctc ggacggtgat cgctggcaag tatttattgc aaatgaggtg gatcattttg
    37921 ttgctgcaca agtaacaaag attggaaagg ttgctgtacg cttggaacca atcaaataca
    37981 cagaaatgat tatgccgaaa gatagctgga cacaagaaag aggaacagtt agttccatgc
    38041 gactagacag tgtgatctct gagatttata atatttccag acagcggtcc aagcagctga
    38101 tcgaagctgg taaagtaaaa gtgaactgga cagaaaatac aagacctgat tttgtacttg
    38161 aactattaga tattatttca gtcagaggtt atggcagagt ccagatccaa gaacttgagg
    38221 ggaagacgaa aaaagaaaag tacagggtac tgttcggtgt attacgtaaa taaaataaaa
    38281 aggtggttta ctcatggctt taactccatt agatatccaa aataagacat tcccgacaaa
    38341 aatgcgggga tataatcaag acgaagtaga tgatttctta gatttagttg ttcgtgacta
    38401 tgaggaattg actcaaagaa atcgagaatt agaaaaagca gtcaaacatt ctgaagaaaa
    38461 acttgagtac ttcaatgaat taaaagatgc tttgaaccaa tcaatcatcg tagctcaaga
    38521 tacggctgat aaagtaaaaa caagtgcaag caaggaatcc gaagtgatcg ttacttctgc
    38581 acaaaacaaa gcagatgagt tagttgcgaa cgctgaaaaa cgtgcacatc aattaacaac
    38641 tgatgcagaa gaaaaagcaa gaaaaatctt gacagatgca acagaaaaag caagacagct
    38701 tgcgacagaa actgaagatc ttaagaaaaa aacacgagta ttccatcaac gtatcagctt
    38761 gatgcttgag tctcaattag aacaagtgaa aagtccggaa tgggatgaaa ttttacaacc
    38821 attctccagc tacgtgactg acagccatga agtgatcaaa gaagttttag caaaacagct
    38881 tgacaatgaa aatgatactg atgtaaactc agacacagga gtcatagaag cacctatgac
    38941 ggcatttgat acacaggcta ttgatatctc gatcgtgcct ggtgaaaatg aaaacgagta
    39001 gaacagaaaa gcactttgtt attcttaaag cgagccgatg agagtggaag ttcggtaaga
    39061 cactgcatgg tgaaagatcc tctacttgag acctgctttt ccgaaattag agtaagaagc
    39121 agcggctgac ctcgttattg gtcacagaga gtttgcacta gatagatgca actaaatttg
    39181 ggtggtacca cggcatgttc gtcccttgcg gctgaacatg cttttttgtg tccggaaaac
    39241 aaataaagga gtgtatggtt gaatgaaaat gaaagaaacc ttgcaattag gaaaaacaaa
    39301 attccctatg cgtggtaatt tgccaaaccg agaaggcgag tggcaaaaag aatgggaaga
    39361 aaatcgaatc taccaaaaaa gacaagagtt gaacgaagga aaacctagct ttgttcttca
    39421 tgatggacct ccatatgcca atggtaatat ccatttagga cattcattga ataaaatcag
    39481 taaagacatt atcattcgtt caaaatcaat gtctggctat cgtgcaccat atgttccagg
    39541 atgggataca catggattgc cgattgaaca agtattggct aataaaggcg tgaaacgtaa
    39601 agaaatgtcc atggcagaat acttggaaaa atgtcgtgaa tatgcacttt cccaagtaga
    39661 taagcaacga gcagatttca aacgcctagg agttgcagga gattgggaac atccttatgt
    39721 tacattagat ccttcttatg aagctgcaca agtacgcgta tttggtaaaa tggctgaaaa
    39781 aggctatatt tacaaaggat tgaaaccaat ttactggtct ccgtcaagtg agtcttcttt
    39841 agcggaagct gaaattgaat ataaagatgt aaaatctcca tctatttttg tagctttcaa
    39901 tgtcaaagac ggcaaagata ttcttgatga agatacagct tttgtcattt ggacgacaac
    39961 accatggaca ttgccagcaa accaaggtat tgctgtaaat ccaaaattta cgtatgtatt
    40021 ggtggaagcc gatggccgta agtttgtcgt agcgaaagat ttgctagaaa cagtccaatc
    40081 agaaatcggt tgggaaaatg taacagttct aaaagaattt gctggacaag aaatggaata
    40141 tatgactgca agtcatccat tctatgaacg tgaatcactt gtgattttag gcgatcatgt
    40201 tactttagac gctggtactg gattagtcca tactgctcca ggacatgggg aagatgacta
    40261 tatcgctggt agcaaatata aactatctgt agtgtcacct gtagactcaa aaggattgtt
    40321 tacagaagaa gcacctggat ttgaaggtat cttttatgac aaagcaaatc ctatgatcac
    40381 tgatctatta aaagaaaaag gagctttgct gaagctagat ttctttactc atagttatcc
    40441 tcacgattgg cggacgaaaa agcctgtgat ctatcgggca actccacaat ggtttgcatc
    40501 tatcgataaa ttccgtcaga atattttgga tgaagtagaa aaagtagagt gggttatccc
    40561 atggggtaaa acacgtttgt ataatatgat ccgtgaccgc ggcgattggg taatttcccg
    40621 tcagcgtgca tggggagtac cacttccgat tttctatgct gaaaatggag aagcaatcat
    40681 cacaccagaa acaatcgaac atgtggcaaa attatttgct gaacatggat caattatctg
    40741 gtttgaaaaa gaagcgaaag aacttcttcc tgaaggattt acccacccag gttctcctaa
    40801 tggcgaattt acaaaagaaa aagatatcat ggatgtctgg tttgattctg gttcttctca
    40861 tgaagctgtt ttacgtcagc gaccagaatt atcattccca gcagatatgt atttagaagg
    40921 atcagaccaa tatcgtggct ggttcaactc aagtatcacg actagtgtgg ctatcaatgg
    40981 tgtagcacca tacaaatcag ttatttctca aggatttgct ttagatggtg aaggccgtaa
    41041 gatgagtaaa tctttaggaa acatcattgc accagaaaaa gtgatcaaac aatttggtgc
    41101 agatatcctt cgcttatggg tatctagtgt agattatgaa gcagatgttc gtgtttctat
    41161 ggatatctta agtcaagtgg cagaagtata tcgtaaaatc agaaatacga tgcgtttctt
    41221 attagcgaat acagaagatt tcgatcctaa aaaagatact gtttcgtaca atgatttacg
    41281 ttctgtagac aaatatatga ccgttcgatt gaaccaagta atcaaagaaa tccgagaaga
    41341 agggtacgac aaatataact tcatgcatat ttaccgtaca gttatgaact tcttgacggt
    41401 tgatctatcg tcattctacc tggattttgc taaagatgta gtttatattg aagcagaaga
    41461 tgattatcaa cgtcgttgca tgcaaactgt tttctatcaa acagctgtag ctttgacaaa
    41521 attattgact cctatcattc cgcatacagc agaagaaatc tggtcattcc tgaaagaaga
    41581 agaggagtac gttcagttaa gcgagtttcc tggttatgaa gagtttgcaa accaagaaga
    41641 attgatggat acctggactg ctttcatgga cttccgtgat aatgtcttaa aagcattgga
    41701 agaagcaaga aacagcaaat tgattggtaa atctttggaa gcaaaactaa ctgtttatcc
    41761 aaaacaacaa gttcgtgaaa tgttaaaagc tttggatgca gatattgcac aattattgat
    41821 cgtttcacca gactactttg aagttatggc agcagatgaa caagtgccag ataacgctat
    41881 ggtatttgat gatgttgcta ttacagtgga aaaagcagac ggggaaactt gcgatcgctg
    41941 tcgccaagtt cgtaaagatg taggagtaga tgaaaaactt cctcatctat gcggtcgttg
    42001 tgcaaaaatc gttgaagact tctatcctga agctgtagct gaaggattcg aagaaaagta
    42061 aaagggaaaa gggggtgtga catgttcttt gtcacacccc cttttgttat caatcgttta
    42121 attttccacg ttcacgatac cagttatctg gttgccagat ccattcgaat ccgtcttttt
    42181 caagtagttc gaaagctgct ttaggtccca ttgatccagc tttgtagttg ggaaaatccg
    42241 gttgtgtttg gtcccaagct tttcgaatga catccacgat atgccaagat tgagcgactt
    42301 cttcccaatg agagaagttc gttccatcac cattcaacgc atctaatagt aatttttcgt
    42361 aagcttctgg gctattttca accatttctg cactatttct aaattccaat ttgattggtt
    42421 ccgtttcaaa tccttgccct acttctttcc cattcaagga taaagaaaat ccttcagttg
    42481 gctgaatata gatcgttaga acattaggtg gaagtgtcgt atcgtcacat ggttcatcga
    42541 cagatgtttt gaatacatta acaggaactt gtttgaatac gatgttgatc cgagtccctt
    42601 tttcagtcaa acgtttgcct gtacgtacat agaaaggaac acctgaccaa cggaagttat
    42661 cgatagtgaa tttgcctgcg acgaatgttt cagtctctga ttgagcatcc actttgtctt
    42721 cttcgcgata gcccttgaat tgtacaccat ccaattcacc ttgagcatat tgcccgcgaa
    42781 cgaagttttc taacacttct tcttcgttat aaatgcggat agctttcaat gctttgatct
    42841 tttctgtacg gatttctttt tcggaaaatg ctactggcgg ttccattgca agtaatgcta
    42901 caacctgtaa aatatgattt tgcaccatgt ctttaagtgc gccactatga tcgtagtagc
    42961 ccccgcgatc ttctacaccg aggttttcgc caaaagtaat ttggatattg tcgatatagc
    43021 gattgttcca ttgagcctca aaaatattat tggcaaaacg gatagctgag atgttttgga
    43081 tcatttcttt tcctaaataa tgatcaatcc tgaaaatatc ttgttctggg aatacttctc
    43141 tgatttgctg gttcaattca aaagcagact ggtaatcaga accgaacggt ttttctatga
    43201 tcaaacgatt gaagccattt tttgtcataa tattttgtga tttcaaatga gaaacgatcg
    43261 ttccaaaaaa ttgaggtgcc atagccaaat aatagacacg gttaccgttt aattgatagt
    43321 gtccatctaa tcggtcagac aattctttta atgtctgata atgcgttgaa tcatttacat
    43381 catgggattg atagtaaaaa tggcttgcaa aggtagttgc ttcttcatct gaaggattca
    43441 atgcacggat tgtttctttg acaacttcac ggtagtgctc atcgctccag ggacggcgag
    43501 cggttccgat tacagcaaaa tgctcgccta aatttccttt tctgtaaagg cggaacaaag
    43561 acgggtacaa tttacgctgg gctaggtcac ctgtaccgcc gaatatcgta aacaatacat
    43621 ttttttcatt agacatgtgg atacactcct ttatatttta tcgacaaaaa tcgtgctggc
    43681 cgccttaaag ctgacgggga ttttctttct atcattttgg atagtgatcg gtccttcata
    43741 agctgcgatg tcaacgatct catattcttc gttgatattc aggtcaatag tgactaagta
    43801 atccaacaat tctttttcat caagcactcg tgcgatacgc acttttgtac cgacaggata
    43861 ggactccaat gtttgtcggc gttcttcatg gacaacttcc tcttttttag gaatcgatcc
    43921 tccatgagga caatgttcag gatgattcaa atagttttct aaatgatctg caagatgttc
    43981 cgaagtcaca tgttccaata cttcagcatc atcatggact tcattccagc tatagtctaa
    44041 atgttcaact aaaaaaactt cccataagcg atgtttgcga atcagactgc tagctttttg
    44101 cagtccgctt gcagtcaatt gtacaccttg ataaggggaa tgttctacaa gctcttcttt
    44161 cacaagttta gaaatcattt cactgacaga tgcagctgag acatctagac tagatacgat
    44221 ttgtttattg ttgactttcg ttgtatctcc acctagttct aaaatgattt tcaaataatc
    44281 ttctcgattt ggtgtcatat tcgatcatcc tttcatggta aaaaaccatc attagtaagt
    44341 gtaacaaaaa aaacgttaat tgactatttt tctaaataaa aaacaaagga tttttagacg
    44401 tttttacaac aaaaaacgaa agatgataga gaagactcat tctttcgttt ataaaggata
    44461 tatatataca ttgggaggga tctatttttc agataacaac ttatatgaag tgtctgcaga
    44521 ttcgtcataa agagtttgtt cgggataaga aaagataaga taaaaaccat actgatcacg
    44581 tacgactaaa cgatattcat ctatttctac cacagatgtg ccaagagact gaccgttgat
    44641 caaaaacgtg taatcagatg taatctgtaa ttgttgtttt tgctggctat cactaaagaa
    44701 ccatgtacct tgatactttg agagtccttc tgacttttcc gcttgttttt tctttttcaa
    44761 gtaaataaaa cttgagagaa cggtaatgag tgaaccaaga aaatagatca ttttttttat
    44821 caccaaatca ctcctttatg agaaaataat cgcatagaca tattctttct tctttatttc
    44881 attgtaccga ataaaagcta aaatttctct aagactttcg actggttttt ttactgtttt
    44941 tctacaaatt atgataggat acatagtagg aagaaaggtg acataatttg aagtatcagc
    45001 aagtggaaat agcttttttt attgaccgcc cgaatcggtt tatagcacat tgtatgaaaa
    45061 aagacggaga aataattatt gcgcatgtca aaaatacagg tagaggaaaa gaggtcctga
    45121 taccaggcgc tgaagtggct attgtatttg ctcctggacc taaacggaaa accgcctatg
    45181 atctgattgc tgtaaaaaag aagggtgact ggtttaatat cgatagtcaa ttgcctaatc
    45241 ggctggcaat tgatggtatt ttagacggca cgatcctttt accaggatta actggtccat
    45301 ttacggtata caagcgtgaa gtaacttatc ttcactccaa atttgatatt tatttagaga
    45361 cggtggtcgg acaaaaagca tttgttgaag tcaaaggtat gacacttgaa aataaagcga
    45421 ttggtgcttt tcctgatgcg cccacactca gaggattgaa acatatagga gaattaacgt
    45481 atgccgcaca agatggttat gccgcttatg tcttgtttat tgctcagttc gaacatcttc
    45541 atctggcaac gatccatgaa gaaatgcaac cagcactagc tgatatggtt cgacatgcgc
    45601 agcaaagcgg agttcaaatc ctcgcctata attgtcaagt atcggcagat gaggtaactg
    45661 tcaagcaggc cattccattt gatgtagata tcccttttga agatcctata caatagaaag
    45721 aaggtaaaat aatgatcaac ttaggtatta tcggaacaaa ttggattact catcaatttg
    45781 tccaagcagc tttaagcaca aaaaaatatg aattgacagc ggtatattct agacatttgg
    45841 aaaaagcaca ggaatttggt tctcattatg aaggggatat cgaattcgca acagatttga
    45901 agactttttt caatattact catatggaca ccatttatat tgcttctccg aatagtcttc
    45961 actttagtca agctaagcaa gcaattttgg caggaaaaaa tgtgatagtt gaaaagccag
    46021 ctttttcgac gccaaaagaa atggatgaaa tcatccattt ggcaaatgaa cacaatgtgt
    46081 atttttttga agcagcaaga aatatccatg aatcaggttt taaaaagatt gccgattact
    46141 taccattaaa agacgagata atcggtgcaa atttcagcta tatgaaatat tcttcgcgct
    46201 atgaccaagt attagatgga gaagaaccga atatcttctc gactcatttc tcaggtggtg
    46261 cattgatgga tttaggtgtt taccttgtat atgcagctgt tgcttggttt ggaatgccaa
    46321 aagaagtcca ttatttccca agaaagattt ctactggtgt agatggtctt ggatggggga
    46381 tcttacgtta tgagaatttt gatgtatcga tccagccagg taaaatcggt gattcttatt
    46441 taccgtcaga gatttatttt gactcaggaa cgctagtgat gaatggagtc aatgcaattg
    46501 aacaagcaca atttcatgat cgcgcgcata aagaaatcat tgatttggcg gtaacagctg
    46561 cagaaaatcc aatggcagat gaagcagagg attttgccaa tgtcatggct catccgaaag
    46621 atccagcttg gggattgttg tatgaagagt ggatcgagct agctagaaat gtgaaccaag
    46681 tgatttatga tttaagaaaa aatggcggca tcaaattcga tgcagataat gaggaagatt
    46741 tttaatgaac gaatttgtac aagtatttcc cgaaaattgg cagaaaaaat atgaggataa
    46801 agggtttatc tcaccttctc ttattcaacg agcagttttt cagccactaa gtaataaaca
    46861 aagtatagta gcggtttctc ctacaggttc aggtaaaaca ctggcttatc tttggcctct
    46921 actactaaac gtggaaccag gtgaagcaag tgcgttggtc atttttgctt cttctcaaga
    46981 actagctatt caagtagcag atgttgcacg tgaatgggga aaagacaaag agttgaaagt
    47041 ccaatctttg gtgggcggag ccaacgtcaa acgtcagatt gagggtttga aggaaaaacc
    47101 agaaatcttg attggtacac caggaagaat attggaattg atgaaagcaa aaaaaataaa
    47161 agcacatcaa gtgaaaacaa tggtatttga tgaagcagac cagctatttg acgctgggaa
    47221 cagccaaatc attgatcaaa ttcttcatca agctccaaca gagtatcagc tagctttttt
    47281 ttcagcgaca gcagatcgtt cccttgaaag cattgaaaaa atcactggga aaacgtttga
    47341 gacaattgac gtgacagcag aggacgattc tcgaaaagga ctgagacatt actttttacg
    47401 tgtaccgata cgaaagaaag atgactactt gagacgtctc acgcatatag aagattttca
    47461 tggtctgatt ttctttaatc aactgactga attaggcgta atggaagaaa aattgctgta
    47521 tagaggtgta ccggtaggaa gtctggcctc tgatcagaac aagctactcc gaaaagcagc
    47581 gctagaacaa ttcaaaaaga aaatggtcag agcacttttg accacagatg tggcggctag
    47641 aggtttagat attacaggtc ttccttatgt agtgaatgca gaagttccgt taagtgaaga
    47701 agcttatttg catcgttcgg gaagaactgg tcgaatgggt aatgaaggaa tagtaattac
    47761 actcgtacaa gataataatc taagagattt aaaacggctt ttacgtccat caaatataac
    47821 acttgaagag atttttttat acggaggtaa gctgcaaact gagcagccaa ccaaagaaga
    47881 tgcagctcct gcagtaaata aaaagtatgc gaaaactttt tctggaaagg agcagaaccc
    47941 agaatataac aaagaaaaaa taagaaaaaa agttaaaaat aaatcaaaaa aagagagaaa
    48001 taaaggagca aggcgcaaat aagcgccttt ttatgctatg attatgaaaa cgggaacaaa
    48061 attggttgcc agaggagggg ttatgtgaaa gaacgtcaag aagagatctt gcagctattg
    48121 acagaaaacc aacaaggatt gacagcaagt gatgtagctg agcgcttaac tatcgatcga
    48181 agcaatgcaa gccgatattt aagtgaacta tacaaggccc atcatattgt aaaaaccgct
    48241 ggccgaccag tcgtttactc tttgccaacg gaaaagtcaa aatctgatga agtgcatgtc
    48301 gactcttcta cgcaagtgac ttttgaaaca ctcgttggag aaaatgattc tttaaaagtc
    48361 agcatacagc aggcaaaagc tgcgattctt tatccaccgc gggggttgca tacgattatt
    48421 tttggagaaa ctggtacagg gaagtcgatg tttgctgaat gtatgtatca ttttgcaatc
    48481 gattcagaga tgctttctgc agatgcgcca tttgtttctt tcaattgtgc ggattatgct
    48541 caaaatccgc aactgctctt tggacacatc tttgggatca aaaaaggggc ttacacagga
    48601 gcagctcaag atagtcccgg tctaatagcc aaagcagacg gcggtatatt gtttttagat
    48661 gagatccacc gtttaccgcc ggaagggcag gaaatgctgt ttactttcat tgataaaggt
    48721 atttaccgtc cgcttggaga aagtgcacaa gtacatgaag catctgttca gatcattggg
    48781 gcgacgactg aatcttcaga aagtttctta tctacgttca atcgacggat tcctatggca
    48841 atcaccttgc cgaatcttgc ttcacgttca ctggatgagc gttatgaaat catctctctt
    48901 tttatcaaac aagaagcgaa tcgtttgaat caacgtatcg atatcgaaaa agaagcgatt
    48961 cttgccttca tgctttatga tgcggaagga aatatcggtc aagtgaaacg agacttgaaa
    49021 ctagtctgtg caaaatcttt tttacattac cgtactcatc atgagaaaaa gctaatcatt
    49081 cgaaaagaag aactttccct tcaagtacaa aaaggattac tgaaaatcaa ggaaataccg
    49141 gaacgtctcg accgattcat ggacagcaaa agtcagtatt tgacatttga accaggattt
    49201 gctgatgttg tctggtcaca agatccagaa cgaaacatgc aggtctataa cgatattgag
    49261 gaaaaagtca cttctctttc cgaaaccggt gtggagaaca ttgatctaga gaccctcatt
    49321 tcaaaagatg tggatgctta tttccagaca tatgtcaaag aattgactaa aagttcgatt
    49381 cccaaagatc ttttgccaga tgaaatctgg cggttaacca atcaattgta tgatgtggct
    49441 gaaaaacggt tagaccgtat ttataatgaa aaagctcgtt ttgcttttgc tttgcatctg
    49501 caaagtacat tggatcgtgt gagagaaggt catatgatcg tccacccaga tttgaataac
    49561 gtacggaaaa attttaagag ggaattccag gtagcaatcg atctttcaac gatcattgaa
    49621 gaagaagaac aagttgagat tccttttgat gaaatcggct ttattagtat gtttctttct
    49681 atcaatctag gagaaagtga gcctgttaaa gaaaacaaag tagatgttct catattgatg
    49741 cacggcagac agactgcttc aagcatgttg gagatggcac aagaactatt aggaacaaaa
    49801 gtagggaaag cttttaatat gccactggtt atggaagttc aaacaatgta tgaagaagtg
    49861 ttacaatatg tcaaaaacta tcaagagcat ttgacgaatg gcctactctt gttaacagat
    49921 atgggatcat taaacacctt cagtgatttg atttatgaag agactggcat tcggacgaaa
    49981 gctatcacga tgacaagtac catgattgtg atagaagcga tacggatggc aagtgttgga
    50041 agaagcttag aagatattta tcaaaatatc cagctttcct ttgaatcgat cgtacgtgat
    50101 cagttcaaag cggaaaagtc tgaaacttta cagcgtaaaa aagccgtaat cgtgacatgc
    50161 tttactggtg aaggagtagc tgccaaatta tatcagcgta ttatgccagt gattaatcaa
    50221 aataaggtag aactgatcca gatgcagttt ttagaacgtg aatcattcaa aaaacacatc
    50281 gatgccttat tggaagaata tgagatcaaa gcaattgcgg gaacagttga tgttgattat
    50341 caaaatatcc catttttttc agcctatgac gtttttgatg atgaaaaact aaatatttta
    50401 aagcgtatag ctgatgacga gatcccgaca gctaaaattg tcgattcact tgctggatca
    50461 ttaagcgatg tcttttctat tgatgaactg atccgttcat tgcaaaagac agttcagcag
    50521 atccagaccg atttgtcgat tatcgtggaa cctggagtag atactggaat cgttatccat
    50581 ttagccttca tgttagacgg attgatcaaa caagaaagcc atcggcaatt tgagaaactg
    50641 tctattttct caaaaattta tcgtttggaa atggatatcg ttcgggcaaa cttgatggca
    50701 ctagaacgaa aatatcaggt aaaaattcca gaagatgata tcgcatttct tacacaaatg
    50761 tttcttgaaa ataaaataaa aaaatgaaca cactaatcat actgtttaca cactaaacat
    50821 ctgtgtgcgc actataaaaa agagctaaaa agcatgaaaa cgctatttca gctctttttt
    50881 ttattggcat gcttcttgcg ttatataaaa gtgtaaaaaa ataaaaggag tgttaaaaat
    50941 ggcaaaaaaa acaatcatgt tagtttgttc agcagggatg agtacgagtt tattggtcac
    51001 aaaaatgcaa aaagcagcag aagaaaaagg gatggaagcc gatatttttg cggtctctgc
    51061 ctcagatgca gataataatt tagaatctaa gaatgtcgat gtattattat taggtcctca
    51121 agtacgtttc atgaaagctc aatttgaaca aaaacttgcg cctaaaggta ttccattaga
    51181 tgtcatcaat atgcaagact atggcatgat gaacggagaa aaagttcttg cacaagcaga
    51241 aaatctaatg aaataaatca agtatgcagt tgggggtata acaacgtgga agatcaacaa
    51301 aatctagaag ccatcatggg gttaatcatg tttggcggta atgcaaagag cgatgcaatg
    51361 gaagcgattg cggctgctaa aatcggtgat tttgagttag cggataggaa aatcgctgat
    51421 gcagaagaat cacttataca agcgcatcat gcacaaacag acatgttgac acaagaagca
    51481 caaggaaatc atatgcaagt gacattattg actgttcaca gtcaagatca cttaatgaca
    51541 tctattgcgt ttactgattt agcaaaagaa atcatcgaac tttatcgtcg cgtatacagc
    51601 gaataaatac aacttctttt ttgaacggaa gaagaaaaat ttttgaacgg attttttatt
    51661 ttgttataag gagaactctc tttatataaa aaacttatag gaggaagtaa aaaatgaacg
    51721 gactatcaag ttggatggag aaatacatcc tgccggttgc cgcaaaaatc ggagcgcaaa
    51781 aacatttagt tgctttgcgt gatgctttta tcggcacact gcccgcaaca atggcgggat
    51841 cagtcgctgt aatgatcaat gcaattattc gtgatttacc gcaacagttt aacagtacct
    51901 atgctgacag tttagcagca gacaaaggaa tctttgccgt attgaatgtc atcatcggaa
    51961 tcaatggttt tgtctggaac ggtacactag caatcgccgg attgatcttt gccttttctt
    52021 gggggtataa tcttgcccga gcttatggag taaatgatct agctgggggg atcgttggtt
    52081 tagctacctt gattcaaggt atttcctttg gtccagcatt atcagcagca ttagatgtga
    52141 atgttccttc aaatgtcgca gatgcaatca atagttctga gattggtgca acgattgcag
    52201 atggcacact gtcagttgga ttatggggtt ggctaaaact agatcatttg aatggaaacg
    52261 cttattttac agttatgatc atgggagcta tttcagtcat tattttttgt aaattgatgc
    52321 ttgccaacat tacgatcaaa atgcctgatt cagtaccacc agcagtatct aaagcatttg
    52381 cagccatttt accagcgacg atcgctttat acgtcattgc tattatcaat tatatcgtag
    52441 gtcgtgtagc tgatgggcaa ctgatcattg atttggtgca aaaatatatt gcagagccat
    52501 tcctaggatt atctcaaggg ttgggagcag tattgatcgt cacagtattt gttcaagtat
    52561 tctggttctt tggtattcat ggaccaaatg tattggcacc agtgctagag ggaatctggg
    52621 gacaagcaca actaatcaat attgatattt tccaaaaagg ttataacgga aaaactggta
    52681 caccagccgt gttagatgca atcgaagatg gaaaagctta tatgtgggtc cgcggatcgt
    52741 tcgatgcttt tgcatggttt ggcggatcag gcggaacgat cgtcttactg atagctatct
    52801 tgattttctc taaacgtgca gattatctaa cagtagctaa attatccatt gggcctggta
    52861 tcttcaatat caacgaacca gtgatgtttg gtctgccaat cgtattgaat gcaatcatgt
    52921 ttatcccatt cttgattgct ccagtagtag caacagcaat tggatggaca gctacttatc
    52981 ttggcctagt cgctccggta tcgcagcaag tcacatgggt agtaccgccg ttcttattgt
    53041 cattcttagc aacaggagcg gattggcggg caccgatcgt aacattagta tgtatggtcg
    53101 tgactttctt gatttgggca ccatttgtca tctctgcaaa caaaatggac cctgccgcag
    53161 gcgaacaaca ataagatgaa cgtgaggagc gggacgcttt aagtttcgtt cctcataatg
    53221 aattttcgag gaggaactga aatgattaca attgccagtg caatgcaaca agatgaaatc
    53281 aaagaattgt tagaaggata caatgaagaa ggaatgactt ttacatttaa agaaaaacaa
    53341 ggaatcaagc tgttctttga aacaagtgca gaagatccgg aagttgcggc taaaaaagca
    53401 aaggaactaa tcaaagcaca gccttgggga acagtccttt attttcaatc aacagctgtt
    53461 tagaaaatgg tttaaaaaga taggatggtt gagatgataa aaaacggttt atttttatgt
    53521 agtattggtt ctttgatcgt gttgtatagt ttgaatccac attcgcaaag gtatgatttg
    53581 tatagtatgg tcacaggtct ttttctaaca ggattaggaa tctatttctt catgaagggg
    53641 aaaaaaagag aagaagcaaa ggagaaaaat aactaatgga aatcacgaaa aaattaaata
    53701 ttccagcatc gtttttttac gataaagtta tggactctgt ccttttcgat gtacgtaaag
    53761 cgaccggcaa atcagtaact agaaaacagc tgaaaaattt ggagtacgtc aaacaattct
    53821 cgcaaaataa ccgggctcgc ataaaaatcg aagaagtcat tgaaaaccag tcttataagt
    53881 ttcgtacatc gacaacgaag aatgaattcg tcgttcatta tcaaatcaaa ccattggacg
    53941 attcgacctg tgaggttcat tacacagaaa aaatggagtc ttatggcatg cttcaaaagt
    54001 tgaatgatat gcttttagga acaatcttga tgtattttaa acggagacgt ttcaaaaaaa
    54061 tgctagatat gatcgaagca acgtattaaa tcaatgaaac atggtactat ttagttattg
    54121 aaaaatgaag taagctagaa ggaatttttc ataggaagat agatctttcc tgcgaatcct
    54181 cactagcttt tttgtaggaa ggggcagcaa aatgacaaac aaagtaagag agctaggtgt
    54241 atcttcgatt gaggaaatgt ttgatttagc cacttatgca tttaatgggg cagatacacc
    54301 tgaaaggcga gaacgttttc gtacacttgc ggagaattct tggaattacg gattttttga
    54361 tgaggaggat cggctaacaa gtcaagtcat ggcgacccct tttccagtga atttttacgg
    54421 acaagagtac ctaatggccg gcattggcta tgttgcttct tatccagaag caaggggaca
    54481 aggcgggatc aatcagatca tggaaaagat ccttgaagat tgccgtgatc gtaaagtaag
    54541 cttatcttat ctggcgccat tctcctatcc cttctatcgg cgctatggct atgaacaatc
    54601 atttgataaa atcagttatc aactaaacag tcgtgatgtg ccgtttatca aaaaaaaatc
    54661 tggaaaaata aagcgtatgg attttgaaga tgcaaaagct gcaatcagaa gaatttatga
    54721 acagatgccg gaaaaccaac gagctggtct gaagcgcgaa gactggtggt atacgtataa
    54781 attcaagcaa aaaggcaatt accagtttgc tttgcatttt gatgataatg aggaagcaac
    54841 gggatatatc gtttatcagc ttgcaacgtc ttcctttatt attgcggagt ggggattttt
    54901 ggatcatgat gctttttgca gccttgttcg cttcgtaagt tctcacaatg gtgcttttga
    54961 aacatttgaa tattcatgcg gctacggcgg taatgatcaa aattatctat tagaaaatcc
    55021 atttgcttct gtaatgatca caccttatat gatggcgagg atcgtagata tttctttgtt
    55081 tttggaaaat tatccattta aaaagagaca agctgcattt gccctacaaa tagaagaaga
    55141 tcattatgcg aaatggaatg aaggaatcgt ggaagtaaca atcgaaaatg gaaaaaatca
    55201 tctctcaaaa gtagaacaaa caaaactccc aatcgttcga ggaagtatcc aacatatcac
    55261 gcagctatta ttagggtatc gtaaagtgga agagttggta ttccaagaaa gaatacaggt
    55321 gacaaaagaa tatgaagaaa cgttaagctc aatcttgcca cagcaacagc caattcttaa
    55381 tgactatttt tgataaaaaa cgggtgatga caatattttt gtcaccaccc ttttgttttt
    55441 catttggcat ttgaaggaaa atgttaaagt aaatcctctt ttttcgtaaa tttgaatttt
    55501 ttgttttctt ttgttttagg gttttctttt ttgtggtctt ggattgcttc tttcgtaatc
    55561 ttttcccgat tttttttaga tgaatctaag cgttctgcgt atactttttc ttttggctta
    55621 tcattcattg gaaaggacct ccctacatta tttcattcta cctctagttt atcatctgtt
    55681 aaggactagt ggaaacgttt tgcctaactt ataagctgat ttttttagca gataagcttg
    55741 caagtaaaaa acaaacaagc tacaattgat ttattgaaat tgttcctgta gctcagtagg
    55801 atagagcaat cgcctcctaa gcgataggtc gctggttcaa ttccagtcag gaacgtacaa
    55861 ataacccctg tatcctttgc ggtacagggg gttattttgt ataagtagac attgtagttt
    55921 ctaaactgct atgtcctaaa cgtttcgata cgctaagtat atttactcct tgataaagta
    55981 aaatagatgc atgcgtatgt cttagtccat caataaacag tcgaagagaa aatagggaaa
    56041 aattgattac taaagaaaaa cttactaggt cgacggtgta gttgattttc atacttcaat
    56101 agattcgaat taaacgattt cgattggctt ataccagtcc tatccaacga aagatatatc
    56161 gttcttaact ctagcggtat aacttgaagc tttacgtaaa gccaatggga atgaactgga
    56221 gatgactttt tattattttt agtaatagct gatgggttaa tacttacaac tttccttttc
    56281 aaagtaccat ataaaaaaac ggtggggttg tttagcgaaa taaaggatct aagccaatag
    56341 cttagatcct ttattctatg ctttatatta ttttttgcat gtgtgcgaaa gtatactcaa
    56401 gcccaatcgc catttcggaa aatcggtact gtagagccat cttcacggat tccgtcgata
    56461 tccatcttgt cagatcctac catgaaatct acgtgggttt gacttctatt caatccagct
    56521 tctgctaatt cttcatcgga catttctgta ccgcctttca cactgaaggc ataagcagaa
    56581 cctagtgcta agtgattcga tgcgttttca tcaaataatg tattgaaaaa gatgatgcca
    56641 gattgagaaa taggtgaagg gtcaggtaca agtgcgactt ctcctaaacg acgcgcacct
    56701 tcatctgtat caagcagttt tgctaagaca tcttctcctt gttcggcaga aaaatcaaca
    56761 acttttccat ctttaaaagt aaatttcata cccgagatga tggtgccagc ataactcagt
    56821 ggctttgtac ttgaaatgta accatctact cgatgactgt caggtgctgt gaacacttct
    56881 tccgtcggca tatttgccat gaatttttct ccacgagcat tatagcttcc agcgccttcc
    56941 caaagatggt ttttaggcaa tccgataatg atatctgtac cgggagcagt atagtgaagg
    57001 gcagaaaatt gttctcgatt caattcttct gctttttttg ctaatttttc atcatgcttc
    57061 ttccatgcta agacagggtc ttcttcatag acacgagtcg ttttgaaaat ctgatcccat
    57121 aatgcctcaa cttgtttttc ttctggcagt tctgggaaga cttttgctgc ccattgttta
    57181 ccagccgcag ccacgacagt ccaactgact ttatttgctt gagttgcctt gcgtaaattc
    57241 attagtgctt tgccattcgc tgattgataa gacgctactc gatggctatc tacaccagca
    57301 aatgcatcgg gatcggcaga aacaacgctg atcctgcttg ctcctttttc aagccattca
    57361 tccgcttgat cgattttaga ttgtggtaca ttctcaatac gatccgttgc agcatgtaaa
    57421 agaaattctc tttggatctg gtcgtcagtc cattgaacga taacctcagc ggctcctagt
    57481 ttataagctt cttgtgtgat caatcgagca aggggtgcct gttccacgct gatctgtaaa
    57541 acaactgtgt gtcctttttc tgtggctacg ccagtttctg caattaagcg tgcatatttt
    57601 tttagaagtt cattaaaatc agataacata ataaaaattc ctcctcacca ttttttctgg
    57661 taactattag catgatacca tttaattatg attcaaacaa tgaggcttta aataaaaaaa
    57721 ggaggttatt tttttaaggt taagtttcat gatatatgtt actacttgct atttcagctt
    57781 gtgaagaaaa tcaaaaacta attttgcata tcaaaaaaac atgttacaat gaaattaatc
    57841 aatggtggaa gcgttataaa aaaattaagg agattaaaaa atatggcaag aaaaaagacg
    57901 attactagag aacagatttt ggcagctgct tatgaagtag ctgccaccga tggatttaca
    57961 cgatttactg cgagaaacat cgcgaacaaa atgaattgtt ccacgcaacc gatttatctt
    58021 gagtttaaaa atatggatga cttgagacaa gctctttttg agcagatcta tgatcattta
    58081 caatatgaag tgttcactgt tgagcataca gggaatacga ttatcgatct tgcttttaat
    58141 tatatccatt ttgcaagaag agagaagaag ttgtatcgtt cactgtactt agaagaatat
    58201 ggtggaggaa aagagatgca gaatttctct tatacttact ttgtgaatac agtgaaacgc
    58261 gatccagctt atgtgaatct ttcagaagag cagatcgaat ctttgtataa tgggacatgg
    58321 atcatagcta ctgggatcgc agctttgatg tcttcaagca tcatccatcc aactgatgaa
    58381 cagattgaaa agatgatcca agacagtata gatgctatct tgaaattcaa agaaccaatc
    58441 gaaatcgatt gaatatactc aaaagttcca caaatcagta ggtaacaaga gatgtgtcaa
    58501 aataaggaaa agttcgtttt tcttacagcg tatgcatttt taactcccga agaactcttc
    58561 catgaatgca atgaataaaa gagccataca gattgaataa atctggatgg ctcttttatt
    58621 gcaagtatat taaatttttg cgactagttc tttagcgaaa gcttctaaat tttcgatatc
    58681 ttcttcttca gcattcagat ccactttaac attttcggct ccttttgatg cgcctgtttt
    58741 tgcaaaagca gcatcgaaat catccaccga tttgcagaat tcatcgtaaa aagtatcacc
    58801 agatccgcag acaccgtaaa tttttcccaa tagatccaat tcttgcaagt cttcataaaa
    58861 gtctacgatc tcatcaggca gatcgccatc atcgtatgta taagtagcta ctacgcaaat
    58921 atctgcctcc tcgaattcat ctgcatctac ttgggtacat tcgttgattt cgacttcgat
    58981 atccagattt tcaaatgctt ctgcaacaat atcggcgatt tcttcagtgt ttcctgtcat
    59041 gctagcatat acgatttttg ctaaagccat gatgtgcctc ctcgtttata attatttaac
    59101 aattatgaat tataccaaaa catcattgaa aagaaaataa aacctaaaga cacctgggga
    59161 atgtcatttg gctaaatgta tgccgctatg ttaaaatgag agggatttgt aaaaaggaga
    59221 cgaaaatatg attactttaa aatcagcacg tgaaattgaa atgatggcag aatctggagc
    59281 tttattagca gatgtacata aaaatctgcg tgattttata aaaccaggaa tcacaagttg
    59341 ggatattgag gtatttgtca gaaactatat cgagagtcat ggcggaattg ctgcgcaaat
    59401 tggctttgaa gggtatgaat atgcgacttg tatcagtata aatgatgaaa tttgtcatgg
    59461 attccctcgt aaaaagcctt tgaaaaacgg tgatttggta aaagtcgata tgtgtatcga
    59521 tttgaaaggc ggcatttctg attcttgttg ggcctatgta gttggagaat ctacacctga
    59581 gatcgaccga ttgatggaag taacgaagaa agctttgtac atcggtattg agcaagcaca
    59641 agtagggaat cggattggtg acatcggtca tgctatccaa acttatgtag aaggcgaaaa
    59701 tctagcgatc gttcgtgatt ttattggtca tggcgttggt cctacgattc atgaagaacc
    59761 agcagttcct cattacggag aagctggaaa agggttgcgg ttgaaagaag gcatggttat
    59821 cacgatcgaa cctatggtta ataccggaac ttggaaaatg aaaatggatc ctaacggctg
    59881 gactgcgtat acaagagacg gcggactttc ttgccaatat gaacatacat tggctattac
    59941 taaagatggt ccaagaattt tgacatcaca aggagaagaa ggcacttatt gatctatcca
    60001 gtcaataggt acgggagagg tacatgaaag tttggaacaa aataaaacaa aatgaaaatc
    60061 tgatgcgctt tattgagacg actcaaaagc gcatcactga ttcagaaatg agtacgacct
    60121 ctgtcgtcgt tgcttattat ttactcttgt ctttatttcc attgattatt gcttttggta
    60181 atattttacc gtttctccac atcgatcaag agactgtact gacctattta cgagaagtga
    60241 ttcctgagac cgtttaccag tttatcggtc ctgcaatcaa agaccttttg acacaaagtt
    60301 caggaggctt gttatccgtt tctgcattag cggctttatg gtctgcaagt caaagtatca
    60361 atgctttgca aattgcgatg aataaagcgt atggcgtaga aaatagaaaa aattttttga
    60421 tcgtccgttt cttctcacta attgtcattg tcttgttcat gattgctatt agtggggtaa
    60481 ctatcgttct tgggttggga cagctgattt tggatgctat tcagccaatc gtacaaatac
    60541 ccgtgaactt tatcgatcag ttccaaactt tgaagtggcc aatcacattg gtagctttat
    60601 ttgtcatcat gttcttgatt tatttaattg tccctaatgc tcagttgaag ttgaagacga
    60661 tcattccggg cacgatcttc gctactgtcg gctggatgct gttatcacaa gtgtttagta
    60721 tctatgctag gtattttgca acgaggatca gcggttatca gatcatagga agttttattg
    60781 tactcatgct ttggcttaat tttgctgcta cgatcatcat tttgggcgga atcatcaatg
    60841 cagttgttca agaatacgtg acgggtcatc aaatcaaaaa acgtcgagat ccaagccgaa
    60901 aatggttcaa taaattgaaa actaaatttt ctcataaaaa aaagtagaaa caagagttct
    60961 tattgtaaat ttatatcagt aatttataat aattatgact cttaattaaa gtaggtgccc
    61021 atatgcttgt ttcttttagt atggttataa gattttttct gacaatttta ttatcactga
    61081 tattaacacc tgtattcaaa atcatttcag tacaaacagg tatggtcgac aaaccaaatg
    61141 aaagaagaat aaataaagtt ccaatgcctt cagcgggcgg actgcctatc tttatttcat
    61201 ttgttatatc gactttattc ttattccgta aaattatccc tcaatcgtat attgtaccga
    61261 tcttgattgc ttctttagta atcatagtca caggagtgct cgatgataaa tatgagctga
    61321 cacctaaaca aaaaagtata gggatcttga ttgcttctct tattatttat tttgtagcag
    61381 atatacgtat tgattccttc acgcttcctt ttattggata tatccaacta ggctggctta
    61441 gttttcccgt aacgattttt tggatcttcg gtatcacgaa tgcggtcaat ctgattgacg
    61501 gtttggatgg gctggcggca gggatttcat tgatcggttt aattacgata ggaatcattg
    61561 gatatttttt cctccatgct tctacagtgt atattccagt tgttattttc tgtcttgtag
    61621 caagtattat cggttttttt ccttacaatt tttatccggc aaagatttat ttaggcgata
    61681 caggagctct attcctaggg tttatgatgg ccgtcctttc actacaaggg ctgaagaacg
    61741 tgacttttgt ttcatcgatt tctttgctga tcgtcatggg agtgccggta acagacacgt
    61801 tttttgctat cattaggaga aaagcaaacc gtgtttcttt ttctacagca gataaaaaac
    61861 atcttcatca ccgactaatc agtttaggct ttactcataa aggtgctgtc ctgacgattt
    61921 atgcgatggc tttgatgttt tctttcacag ctatggtcat gaattatact ggaagaatcg
    61981 gaacgatcgt attgattatt gccatgttgt ttgctgcaat tttattattt gaattgattg
    62041 gtctgatcaa tgagaaacat cagtttattt taaaaacatt gcgattttta gggaataaag
    62101 agtaccgtac gcaagtaaaa cagaaatatt tgaagaaatg gaaccgtcat atagatgaat
    62161 aacaaattaa accagccgat ccttattagt attgtgacgg caaacagtga taaaattttt
    62221 cggacactag ataattttat ttcaactgta aaagatgaaa cagcagtaaa aatccgaatt
    62281 tttgacaaca attcacttcc cgagtacaaa aaaagactaa cagagtatag gaaatatccc
    62341 tacatcgata tctattttca tgatgaaaat tccggatttg gttatggaca taatcacaat
    62401 ctgatagcta gcgattctcg ctatggcatt atttgtaacc cagatatttt agtggatgaa
    62461 gagacagtta cagcattggt cttgttattg aaagaacatc cggaatgtgc gatgttagca
    62521 cctaagattc ttaatgaaga tggaacaact cagcacctca tccgtgagca gctggctgtt
    62581 tttgattacg ttttgcgctt tgtgcctttc aaatgggtca aaaaaaagtt tgacaaacga
    62641 ctggctgtgt ttgaatgccg aggattgcct actgatgcta atagttatgt acggatgatc
    62701 tcaggtagtt ttatggtagc tgatattgat aaatttaaag agataaaggg gtttgatgag
    62761 cgtttcttca tgtactttga agacaacgac ttgtgtttga gttttgaaaa agcaggttac
    62821 aagttattgt acacaccctt ttatgcagtt atccattttt acgggaaggg tgcgcatcga
    62881 aatgcaaaac tgtttcgcgt gtttttgact tctatgatga aatttttcaa taagtggggg
    62941 tggcgttttt tctgatgaaa ccagaaatat tagtagtcgt tgtgctttat cagcaactat
    63001 tcagtcaatc tccagcttat ggaccattaa gcaaagcact tagcgaaaag caaattcagc
    63061 ttattattta cgataatagt cctcaaaggc agcaaaatga gctgtttgaa aaagaaaata
    63121 tcgtttatca tcatgatccg caaaatcctg gtttagcggt ggcttataat tttgcgttgt
    63181 ctcaagcaga tgaaggaaca cgttgtctcg tcactctgga tcaggatacg aggctagtcg
    63241 acgattattt tgaactattg cgtaaagtaa cctttacaga tgaatgtgtt gctgcagttc
    63301 cgatgatttt ttctgggagc cgacaagttt ctcccctata ttcagatcgt tatatcaatc
    63361 gtacagctca agcagtagaa gttgaaggcc caacagctga acgaatcatg gcaatcaatt
    63421 caggtgttgc ctggtctgtc aaatttctaa aaaagattgg cgggtttaat cctgcttttt
    63481 ctttagattt tttggatcat tggctgtttt ggaaagtgga tcagttggaa aaaactgtgg
    63541 agatccttcc tgtacgaatg gaacatgatc tttcggtttt agactatgaa aaagtttcaa
    63601 gtaatcgata ccgttctatt ttacaagcag aaaatcgatt ttatcatgaa tatgatcaaa
    63661 ggcatttatc cagtcaccga cgacaattat tgttaagaac agctaaacag ttcttgaccg
    63721 tcaaaaaccg gcatatctgg cggatgactt tgcgctcata tgttaagaat tggaaggtgt
    63781 gaattcgtgc gttcggtttg tatagctacc tttaatggag gcagatattt aagagagcag
    63841 ttagatagta ttttatctca gctttcagca gaagatgaag tagtcatttc tgatgatggg
    63901 tcagtagatg atacatggga tatattaact gaatacgcag aaaaagataa gcgaatccag
    63961 cttttttcgg ggcctcgtca aggattgatc gctaattttg gttatgcgat ccagcagacg
    64021 aaaggagatc taatcttttt ggcagaccaa gatgatgttt ggttggagac aaaagtgaaa
    64081 aagatcactg actattttga gcagaatccg gatcagctag tagtagtttc tgatttaatc
    64141 attgtggaca gcgagctgca agagatccat gattcgtatt tttcgtttcg aaattccaaa
    64201 gaaggatggc ttaataatgt gataagaagt cactatattg gtgctggtat ggctttccgg
    64261 tcttctttaa aacccgatat tttgccgatc ccgtcagaag tgccgatgca tgatatgtgg
    64321 attggtttgc tgggaggaaa gaagactggg ttcctaagag aaccgttaac tctgtaccga
    64381 agacatgatt taaacgcgag tgagattgaa acacattcta aattacggca aaaaatttct
    64441 tggcgtgttc atctgattac ggcgttgatc aaacgaaaat ggtttaaacg ttgataaaag
    64501 atgtttaaat ccttaatatt tccatgacaa atataacaat tgatgagata ataacaaagt
    64561 gaaatttgaa atcaaaaagg agagcagata tccatgaagg gaatcattct tgctgggggc
    64621 agcggaacac gtctgtaccc tttaacaaag gcaacatcga aacaattgat gccgatttat
    64681 gacaaaccaa tgatttatta tccaatgtct actctgatgt tggcgggaat caaagaaatt
    64741 ctgattattt caacgccaca agatacacca cgtttcaagg aattatttgg aaacggaaat
    64801 gatttaggac tgcagatcga atatgcagta caagaaagtc cagatggatt ggcacaagcg
    64861 tttatcattg gagaagaatt tattggagat gatagtgtct gcttggtatt aggggacaat
    64921 atttactatg gtggcggtct gtctaaaatg ttgcaacgag cagcttcaaa agatacaggc
    64981 gcgacagtat ttggctatca tgtcaatgat ccagaacgct ttggtgtagt agaatttgat
    65041 gagaatatgc aagctttgtc gattgaagaa aagcctgctc agccaaaatc aaattacgct
    65101 gtaactggcc tttattttta tgacaatgag gtaatttcta tcgctaaagg tatcaaacct
    65161 tcagaacgtg gagaactaga gattacggat gtcaataaag catatttgga taaaggaaaa
    65221 ctatccgtag aacttatggg acgcggattt gcttggcttg atacaggaac acatgaatca
    65281 ttgcttgaag catctacctt tatcgaaaca attgaaaaac gtcagaactt aaaagtggct
    65341 tgtttggaag aaattgctta ccgtatgggc tacatcgata aggaacaatt gctggccctt
    65401 gcacagccat tgaaaaaaaa tcaatacggt caatatttat tgaatttggc cgctgaataa
    65461 ggagtcctta cagatgaaag taacagatac aaaattaaca gatgtcaaaa tcgtagaaat
    65521 ggacgtcttt ggcgatcatc gcggtttctt cacagaaagt tattcaaaag caaaatttgc
    65581 agaacatgga ttgaaatatg actttgttca agacaaccat tcactatcag ctgaagctgg
    65641 tgtccttcgg gggcttcatt tccaaaaagg agaagctgca cagacaaaat tgattcgggt
    65701 agtcactgga gcagttttag atgtagtagt ggatatccgc aaaggtagcc cgacttatgg
    65761 tcaatgggac ggatttatct tatctgaaca taatcatcga cagttgcttg tacctaaagg
    65821 atatgcccat ggattcgtta ctttgacacc aaatgtgaac ttcctttata agtgtgataa
    65881 ttactataat gcagaagcag atgggggcat cgcatttgat gatccagatt tagcgattga
    65941 ttggccaatt gatccatcaa gagcgattat gtcggaaaaa gatcaccgtc atccgacatt
    66001 aaaagaattt gaagcagaaa atccatttgt ttacggagaa atctaagaat taaggagaaa
    66061 ttttcttatg aaaaacatca ttgttaccgg aggtgccgga ttcatcggct ctaatttcgt
    66121 tcattacgtg gtaaacaatc atccagaagt gcatgtcact gttttggata aactgactta
    66181 tgcaggaaac aaagagaact tggccggact tcctagcgat cgcgtagaat tggtcgtagg
    66241 agatgttgca gatgcagaat tagtggaccg tctagtaaaa gaagcggacg cagttgttca
    66301 ttacgcggca gaatcccata acgataattc attaaaagat cctttcccat ttgtacaaac
    66361 gaatatcatt ggtacgtata cacttttaga agcttgccgc aagtatgatg tacgctatca
    66421 tcacgtatct actgacgagg tttacggcga tcttccatta cgtgaagacc tgccaggaca
    66481 tggggaaggc gaaggagaaa aattcactgc ggaaacacca tataatcctt caagtccata
    66541 ttcttctaca aaagctggtt cagatctgtt agtaaaagca tgggtgcgtt cttttggctt
    66601 acgtgcaacc atttcaaact gttcaaacaa ctatggtcct tatcagcata ttgaaaaatt
    66661 tattcctcgc caagtaacga gtatcttaag cggtattcgt ccaaaattat atggcgaagg
    66721 aaagaatgtt cgtgactgga tccatacgaa tgatcattct tcagcagtgt ggctgatttt
    66781 gacgaaaggc agaattggcg aaacctatct gatcggtgca gacggtgaag aagataataa
    66841 gaccgtgatg gaattgatct tagaaatgat gggccaacca aaagacgcat acgaccatgt
    66901 aaacgaccgc gcagggcacg atttgcgtta tgcaatcgat tcgacaaaat tgcgagaaga
    66961 gctaggttgg cagcctgagt ttacgaactt ccgtgatggg ttggctgaca cgatcaaatg
    67021 gtacgaagaa catgaagact ggtggaaaaa agaaaaagag gccgttgaag cagcatatgc
    67081 caaaaatgga caataataca gaggaaattt cactgtatta atggaaaaac acatgtgagg
    67141 caggcgctcg cctgtctctt ttttaaaata aaaatcaagg agcggataac catatgtttt
    67201 tattaactgg aggaaatgga caattaggta cagaattgcg ccatttatta gatgaaaaaa
    67261 atctgccata tgtttcaaca gatgcaaaag acttggatat cacagatgct gagaaaacaa
    67321 tggcttatat tacagaatta aaacccgatg ttattttcca ttgcgcagct tacacagcag
    67381 tagataaagc agaagaagaa gcaaaagaat tagatgagaa aatcaatgtc gatggcacaa
    67441 gaaatgtagc acttgcagca aaagctgtga atgcacaatt cgtctatatc agtacagact
    67501 atgttttcga tggaaaaaac aaaaatgtag aatacgaagt agacgaccaa acgaatccac
    67561 taaatgaata tggtcgtacg aaattattag gcgaaaaagc agtagaagag attttagaag
    67621 actattatat catacgaact tcttgggttt tcgggatcta tggacacaat ttcgtcttca
    67681 cgatgcaacg cctagcggaa acccgcgaca aactgactgt agtaaatgac cagtttgggc
    67741 gaccaacttg gacacgtact ttggcagaat ttatggtata tgtcatcgaa gaaaaagcac
    67801 catttggtat ttatcatttg tcgaacgaaa atagctgttc ttggtatgag ttcgcaaaag
    67861 aaatattgaa agatacagat gtagaagttg cacctgttac gtcagaagaa tttccgcaaa
    67921 aagcaacacg tccgcagtat tcagtcatga gcctgaaaaa gacagaagcg ttaggttttg
    67981 tcataccaac ttggcaagaa gcacttgcgc aaatgctgga agctgccaaa aaaatgaata
    68041 agtaatttcc tttttatcaa atagaatttg gagcatgata tggaaaaaca aacaagaagt
    68101 gcacgacgaa aagaaaagcc aaaagcgaat caggcagatt cgaatttggt gcttgacctg
    68161 tttctaaaac tccgctatct tattggcgtt ttagtcatcg tggtcatcgt tacttttaat
    68221 ttaaacggaa gttcacttgg tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa
    68281 aaaactgatg tgatttttgg acagaatcgg gaagtgcggt cagacgaatg gctggtgcag
    68341 acaccttttt acctctcaca agctgaagag gacttttcgt tggtgaacga ggattattca
    68401 ttaaacggac aaaacatgat cattgcgtat aattctcctg taaaggatat tacggttatc
    68461 ggaaaaccat tcaactgggg attctttttc ctcggaagag atcgaggact ttccttttat
    68521 tgggcgatga aattagtcgt catggtcttg cttggattcg aagtactgat gatattaacg
    68581 aagaggaaaa aagctctatc gctaatcggt gcatttgcgc tgacattttc tccagcagtc
    68641 cagtggtggt tcatgcaaca tgtgggcgat ttgattttct ttactttggg actgatgatt
    68701 gctttttatc attatttcta caatcatgat aaaaaatggc tacgaacgtt gatgatgctt
    68761 ttggtggtta ttttcggtat tgggtttatt ttagtgattt acccagccca tcaagtgatg
    68821 ctggcgtact tgctgatttt ttattttgtt ggcctgctta tttactatag caagaaagtt
    68881 tcatgggatc tgttggatgt agtgttgatt ggaggggcag ttttatttat tggcggagtc
    68941 atggttcatt tctggctaac gtcaaaagac gcattgttag cttctttgaa taccttatat
    69001 ccaggaaaac gggtaagtac tggaggaaag tggacgattg gcgaattttt ttctttcctt
    69061 acaaattgga aaattccttt tgaagatatc aactattcga ataactctga agtggccctg
    69121 ttctatcact tttttccaac tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa
    69181 gatagcgaac aaaagttact gggacgtatt ttgatgattt tttgtctgtt ttctatcttt
    69241 tggattaccg ttgggttgcc aaaatggcta gcagaaatca ctctgctgtc ttatgttcct
    69301 ccattaagag ccatccttac ttttagtttt gcggcttgtt tattatcgat ctggtttatc
    69361 gcttatattt ggagagaaga tgttattcct atttggtata aattcatttt gttggcagta
    69421 aatgcatggt cttatttcta tgcgattacg cattcgaaaa tggataatta tttttcggat
    69481 tttgaaacca tcgcagttgc tgtgttggga acagtgattt tagcttttgc attatttggc
    69541 tggagacgct tgttttacct cgcttttgtc gccttagtta tcgtatctgg ctttttcgtc
    69601 aatccagtcg tagaaggtac aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa
    69661 aaaatcgatc aacaagatcc tggtaaagtt tggttgtctg aagatgaatt atataacttc
    69721 accccaacat tgggagtgaa atccttcaat acagtacgct tttatccaga tatagctgca
    69781 tggaagaaaa tcgatccgaa agaagaatac gagaagtatt ataatcggta tgctcataca
    69841 agagctttta tcgctaagga agatacaaag ttcattttag atcgtccaga tatgttttcg
    69901 gtcacgttga attttgaaga cgcagaaaaa ttagggatcg actatgtgat cagtaaacga
    69961 ccattaactg attacaacca attatatgaa gcgcagttca cgcagctata tggaccggat
    70021 aaagacggtt atatgatttt taaagtagaa tatccggatt atccagatgt accacaaaca
    70081 caaactcagg aaaatcagct agtcgtacag taataggcta gctgagaata atcctgaaat
    70141 attagtagaa aggagagtga agataattca agcaatcgta caaaaatttg tcaacaaaat
    70201 gagtgtcggt ttcttgcttt tgttgctgac gctagtcttt tttgtttgga cagcaggggt
    70261 aggttttcca gcaactttgt ttatatataa caatagttca ttacttatcg cttttagtgt
    70321 ggctattttg ctcattttaa atgtttataa agcaaaaggc gtcgattttc tttatctagg
    70381 tgttgcgctt gcccttttca ttttcttttc ttactttcgg gaattaagaa atgagtcttt
    70441 atttggagac ttactgtttc ctttggttat tttattttgt ttagtaatta aatggatcga
    70501 gtggacaaaa tgggatcgcg gtatttatct gattgtcttt acggtcttcc tcggaatcac
    70561 gttatatcgt gtatttacag agatccaagt accagaagga caaagcatct gggctccagg
    70621 caattcgctt tctgatattt ggatcaatac gaatacaatc ggaagttctt tgatgactct
    70681 tgctttattg atcagtggat ttgccagttc ttttgaacga tggtatgttc gaatcatcgg
    70741 tgggatagca gtcgttgcag cctttttagg tatttgggtc tgccaatcta gaggtgcatt
    70801 agtcgcgttg attgtttttg cgatattgga tcttttacct aaatcgttga tgcgtgtcat
    70861 tcgtgcgcca tttattgcct acacggttac gattctttta gctttgccga tttcttatct
    70921 ggcggctgtc tctgaaaaag tcaatttgtt taccgatcga gaagatattt ggcataaatt
    70981 ctatcagacg ctaggagaaa aatctgaaca agttttacta ggaatgaaaa catttatctt
    71041 ccagcgagga aaccaatttt taggaaacca caatagttat aattctattt tgaatatcta
    71101 tggattgata ggatttggga ttgctgcttt gctgcttatc ctttttattg gtcgcttaac
    71161 gttgaaagca gatttatcca acgggcagat gacttttatc tgggcctttt ttgcggtgat
    71221 gatgcagtca tttatggagg atacattgac ttcataccca tgggttccga ttatttatat
    71281 cctgttggct atggccgttc atcgatatga tgaaccagaa gttgcggagg aattctatgt
    71341 tatggaagag gcatatgacg atatgactta tgatgataca tcttatgagg aacaaccaga
    71401 tagcgctcat tcttccagga tccaacgcta tcattgatag gcacaaaata cgagagaggc
    71461 tgtgacaacc gtcgcagcct tttttggttt attgcaattg tctagcatct tctgtaaaat
    71521 agaaacgtta gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc
    71581 ggtaccaaaa atatggtaaa atggcacttg actgtaattc actgaaaatc gcttggagga
    71641 acacaaatgt ttaaagattt gtatttattt atgaaggaca tttatcagaa cagaaaatta
    71701 ttgctacagt tttcgatcaa tgacttcaaa tcaagatatg caggatcgtt tttaggaatc
    71761 ttatgggcgt ttatcaatcc gctatttact gtattgattt attggctggt atttggtgtt
    71821 ggactgaaag cacaaatgat cgatgcaaaa tacccattta tcgtatattt agttaccggg
    71881 atgattccct ggttcttctt ctcagatgca tttgcatcaa ctacggttgt ttttcgggaa
    71941 tatacttatt tagtaaagaa agtcgtattc aatattcgta tcttgcccac gacgaaaatc
    72001 ctatccaatc tgtacaccca tttattcttc attctgattg gcatagctgt cgcgcttata
    72061 aatggtatat atccttcagc aatgtcttta cagctgattt attatttatt ttgcatgtgt
    72121 gctttcttga caggattgac ttggctgacg gcatcgatcc aaccattttt acctgatatc
    72181 atgcaattaa ttacgatcgt gctgcagttg atcatgtgga cattaccgat attatggagc
    72241 cctaatcagt ttgattcagg tcttgtaagc atattgaaat taaatccgct gtactatgtt
    72301 gttcaaggtt atcgtgagtc atttttaagt gaagcttggt tctgggagca tggaacatat
    72361 actttgtatt tctgggtttt cacgttagta atgttcgtaa tcggctctgc agtgttccgt
    72421 agattgaaac cacatttttc agatgtgctg taaggagaat agctatgaca gaatatgcaa
    72481 ttagactaaa agatatcaca aaatcttata atatgtacgc aaaaccttcg gatcgatttc
    72541 gtgaagccct caatcctttt aagaaatcct atcatgatgt attttatgcg ttgaaaaacg
    72601 tgaatgtaga aataaaaaaa ggtgagatga tcggtttcgt gggagaaaac ggatcgggaa
    72661 aatctacaat gttgaaaatc attacgggag tactgacacc aacatccggc acgatggaaa
    72721 tcgaaggaaa aatctcagct ttgctagaac taggatcagg attcaatcct gagtattctg
    72781 gctatgaaaa tatctattta aatggaatgg ttctaggatt ttcaagagaa gaagtcgatg
    72841 aaatggtcga tgatatcatt gaatttgctg acatcggaga ccatatttat caacctgtaa
    72901 aaacttattc aagcgggatg ttcgttcgtc tagcttttgc tgtagcaatc aatgtgaatc
    72961 ccgatatttt gattgtggat gaagcattgg cagtaggcga tcttgaattt caactaaaat
    73021 gtatggaaaa atttacagaa atcaagaact cgggaaagac gattttattc gtttctcatg
    73081 atatcagttc gattcgtcgt ttttgtgatc gttcctattg gttgaaaaac ggagaagtcg
    73141 tcgaatatgg cgatacgatg gaagtaacga cgaattatga aaacttttta aagaaaaaat
    73201 cagttaaaac agtggatcgg aataaaaacc aagtagaacg gttcgccgct ccagatatcg
    73261 tcgacgtgac gaaagcggaa ttgttggata aagacaaaaa tccggtggat atgctggaac
    73321 aaggagaaaa actatacgtt aaagtaactt atgaagtaaa agatgataca atcaaaaatc
    73381 cggttttagg aattgcgcta cgaacagtcg acaattttta tgtatgcgga cttaatactc
    73441 tactagatgg tatcaagatt ccttggaaaa aaggagagaa tatcttttat ctggagtatg
    73501 aaaagttagg tttgttagct ggtgaatatt attttgacgt tgcggtcttt gaagaaaatg
    73561 caacggttcc tttggtatat aagacaaagt acatgaactt gtttgtcagc ggttcttata
    73621 tcggagaagg catcgtggtc ttagaccata aatgggaaga agggactcat agcaatgaaa
    73681 tatgattttg aaatggacct agatgaacaa agcagtgtgg gaaaaattgc cgcacagatc
    73741 aaaccaggaa gtaaagtttt agagtttggt cccggaaacg gccgattaac gaaacattta
    73801 atcggagcaa aacaatgtga agtgagcatt gttgagttag ataaggaatt gttcgatttt
    73861 gtcagcgaat ttgctcaaga tggattttat ggtgatatcg aaagttttga gtgggcaaac
    73921 tattatgctg gacagacatt tgattacgtc ttgtttgcgg atgttttaga acatttggta
    73981 gatcctggaa aaacattgaa gaaagtcaga gaatttttga atgaagaagg agagatcctg
    74041 attacattcc ctaacttggc gcataattct gtcatgatcg atttgttcaa taaccaattg
    74101 ccgtgggctt cttatggtct cctagatgaa acccataatt ctttttatac tcatgatggc
    74161 ttccaaaaag tttttgaaaa agcgggatta ttcattaata ttgaagatta tttgtactta
    74221 gctgtaggtg atacagaatt aaaatcaact tatgaagaat tgccggaagc tgtacgctat
    74281 gatttcaaaa tgcgaccatt tggtgaagtc taccaatact ttttctcatt gatgaaacat
    74341 ccagtagcac aaagttccat tgcagagcca caaaattcta attatgtaaa agtgttagaa
    74401 gtaacccagc aaacgaaaca agatgaaaca atccaacaaa taccattcaa taatttcaca
    74461 ggggaaaatg agacactaag tttccccgta tcagaaacaa cagaaagaat ggtgtttcgc
    74521 ttcgcgcaac aacctagctt tatcgaattc agcgcagaag ctgccggaga aaaactaaca
    74581 tttatcgatt cgaatgctgt agtcaaaaca gtaaatgatt gttatttgtt tgatgggaaa
    74641 gaattaccag agttcgttct aacggatatt tctgggaaag aagtgactat ccattgtcac
    74701 tatcgcttta tcggggaatt gacaccaaca atgaaagaat tgttggaaac gattcgtccc
    74761 atggctgaag tcacaaagca gctttctgag aaaaatgacg aattagaaag agaaaatcat
    74821 catcttcttg aggaaaatac aagactagac catacgttaa aaacaaccac aaatcgctat
    74881 tgcacattgt tggaaagtga tgaatggaca ataaaacata gactaggacg acgtaagaaa
    74941 gaaacttcga aaaaaatcca agaaaaagaa ctttctatat gtattgacga gaaaatctgg
    75001 gatgcagaaa caaaaatatt aaaaatcatc ggatggggaa tcgctaattc ggatcgccag
    75061 cccttgtcct ataagttatc agctgaccaa tcgccatttt tcgaagcaat tcctgtttca
    75121 cgagaagaag tcaatcaagc agaacagctc gcaaaaggaa cagaagcggg atttgagtta
    75181 cgaatccttt gcgagcaaga acgttcattc ttagtggaag cagtagctca aaatggccaa
    75241 tcatggatca ttgaaatgta aataatgatt aacttataaa gataaggagt aaacgaatcg
    75301 tgcaaaaaaa agaaattgcc gtatttatcg atcaaatcac tcgtgaacgg gccactggcg
    75361 atttgcttat tgtgggctgg gcaattgatg aagtgaccaa agaaatccct acgataaagg
    75421 tagaaaaaga aaatgtgatt gctgaagcaa cacatgtcgt aagattggat atcaatcatt
    75481 tatataatct tgatgttaag acccaaagcg gtttcaaggt acgtttatca gggaaaatga
    75541 gaggaaaagc catcttagac ttccaaacag cgagacatca aaatggcata gctgtgaaat
    75601 taaacggtaa atacccttat gatgacggca ttgaatcttc atgggaaaga aaaaaaagat
    75661 tactgaaaaa agggataaat tatgcaagga ctcatggcgt aaaaaaagcg atccgtcggg
    75721 tcaaattgga attgaagcca ggatcgatcg actatgcaga gtggatcagt cgtcatgaga
    75781 aaccagattt gaaagagcaa aggaaagaag cacaaggatt tgcgtaccgt ccattgattt
    75841 ccatacttat gcctgtctat aacgttgaaa tcaaatggct cgaaaaatgc attgattcgg
    75901 tactggatca aacgtatgat cattgggaac tatgtatcag tgatgatgct tctactgatc
    75961 cagctatccg aaagtgtctg gaatcctatc aagcaaaaga tgatagaatc aaagtcgttt
    76021 tccgaaaaga aaatggacac atcagtctgg caactaattc tgcattggaa atagcagaag
    76081 gagaatttat agcactctta gataatgatg atgaattacc gccatttgca ttatatgaag
    76141 tggcaaaagt gctaaacgta catccagaac tggatgtgat ctacagtgac gaagataaaa
    76201 tcgatgcaga tggcaatcgc tttgatccgc atttcaaagc agactggtcg cctgatacat
    76261 tgatgggaaa taattatatc tctcacttag gcgtatatcg aacaagcatt gttaaagaac
    76321 ttggcggttt ccgtaaaggc tatgaaggtt ctcaagatta tgatttggtc cttcgtgtga
    76381 cagaacaaat ccctgcagat catatttacc acattgaccg agtcttgtat cattggcgga
    76441 cgatcccagg atcaactgca agtaatggcg aagccaaaag ttatatctat gattccggag
    76501 tgaaagcatt gacagatgcc ctgtctagaa gaaacatcaa aggaagcgta cgtcctggca
    76561 gaatttctgg attttatgag attgcttatg acgtcttgca agaggatttg gtcagtgtga
    76621 tcattccaac gaagaatggc tatgaagatc taaaaacttg tgtggattcg atcatcgaaa
    76681 aaacgtctta ttcaaattat gaaatcatta ttgcggataa cggaagtaca gatccaaaaa
    76741 tgcaagaatt atttgcagaa tacaagcatc agctgaaaga ccgctttatc gtagaattga
    76801 ttgacattcc attcaattat tctcgaatta acaatttagc agcagaaaaa gcaaacggca
    76861 aatacttttt attcttgaat aacgatacag aagtgattga gccagattgg atgacggtga
    76921 tggtttctta tgcacagttt gatcgaatcg gctgtgtagg agcaaaactg ttttatccag
    76981 atgatacaac gcagcatgca ggagtattat taggaatcgg tggtgttgca gggcatgcat
    77041 tgaataacta tgatcgaaca cattgtggtt atttcggccg attggtcatt gacgtcaatt
    77101 acttagcagt cactgcagct tgtatgatgg tcaaggcagc tgacttcaat gctgttaacg
    77161 ggtttgatga aacattagaa gtagcattca acgatgttga cttgtgcttg aaagtttatg
    77221 aactgggtcg ttacaatgtc tatgcacatc aagcagaatt gtaccatttc gaatctaaat
    77281 caagaggtta tgaagatact ccagaaaaac aaagacgttt tgctggtgaa atcaaaaaaa
    77341 tgcaagataa atggccagca tatattgcgc atgatccatt ttacaatgat aatttaacaa
    77401 aagacggaat cggcgacttt tcgttgagac cagattaaac tataataaaa agctgttatg
    77461 ttgtgattcg ctcgcacata acggcttttt atagtttttt gtcaaaaaaa ggcagaaggt
    77521 gccgactcaa aaaaataagt cgaggttggg acagaagtgt ttaactccga gaaataagaa
    77581 gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc ggcttattta agaagaagtt
    77641 gcttctgctc ccaccgttta ttcgttttta aagaatgaga caaaagtgtt tcttactttt
    77701 gtcccacgct cttattcatg ctcagagcgc caatagatac aaaatcatag ccaaagtttc
    77761 acgaacccta gctgtcaaag aatcgatatt ttattttaat ataggctatt ttaaaataaa
    77821 tggtttaagg tattcctcta caaaagccac tctatcaatt atcatgggtt taaaataaga
    77881 agtgatggag ataaccagat tgttcttagc gtcaatataa agaacatttc cactattacc
    77941 aattgctgaa tatatttcgt tctcagaatc taatacccac cataaatagc catatctcat
    78001 attttgtaag tcttctaaag cactggttct aggtttgaac atctcgttta tccacatggg
    78061 tggtacgatt tgttttcctt tatacatccc tttatttagg cataatacac caatttttgc
    78121 cagatcactt gccgataaac acaaaccata tcctgccgtc ccgatattta gtggatcaca
    78181 aaaccagaca ttggttttag gagacttatc cattgtaaac tgcttatgtt cttcagctgt
    78241 ttcagcataa taattgacat ggtttggtat ccctatagga tcaaatagat acttattggc
    78301 aaaatcaaca gttgttagct tacttgtctg atacaacaag ccagttaata tgtgtaaaca
    78361 gacagtttga tagttgaatt cgccggttat accttttctt cctcccaata aatctagtga
    78421 tgtttttgtc caatcttcac ttgaacagac ttttgtccat ggatcgcctt tacatttgta
    78481 gggtgctttc attgttagca agtgcttgat tgtaatagcg taaatagttt tctccccgcg
    78541 tttcacttta tactctggaa aataatctaa tactttatca tcgatacttt tgattaatcc
    78601 ttgctctaaa gcaatgccaa tcagcaaaga tacaatactt ttcgttgctg acatgatatg
    78661 acaagtatcg tcttttttat aaccattcca ttcatctgta taaacttcta ccccatcctt
    78721 atatgcgaca atttgacata tatttggttg tcgaaaacca ataaaatcat gcaactcttg
    78781 tttattcata agatattacc tcactttctt ctataagcat aatgtcatta aaaatcgatt
    78841 tcaatttttt cgattcttca cgtaatacag gtattcttat ggactagaca gcgctttggg
    78901 aagatggatt ctctgaaaaa tataaaggtt cactttatga gtggataaaa aaagcagcaa
    78961 atacttcgac ttctttaatt taccaacatt agtgaaaatc gattcatctg gagatttgta
    79021 atttagagtc cttgtcttga ttagaggtgt ataaagcttt ttattagaca tttttaaatt
    79081 ccattacaat ttgcttataa actaggtttt taaaggtgac tatggtaaga taatagagat
    79141 attattttgt gattaggagt gtatatatga ataaaaatgg agaatggagt gctgcacgac
    79201 ggatattcat tatattaatg gatgttattg tatacaatgc agcaatatat agtagtttct
    79261 tactgaagtt tcgtggagag atcccattac gaaatttaga aactttccaa cactcagcaa
    79321 ttattatttc agtgattttt attgcgttga acattttgtt gggtgcatat gttttttata
    79381 atcgaatgat cagtgacatc gtgtttgtca cagtcatcgg tcaggtattg atgacactag
    79441 ggatcatggt agtgactttt gcgggccgct ggtttgcttt tccacgtctt gttatcttac
    79501 tttcatttgt tctcggtacg cttttcttaa gtatctatcg tattctcgta tataaacttt
    79561 atttacgcgt aagtcgagat aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac
    79621 cagcaatcca aaattttggt tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc
    79681 ttgataacta ttatgcaaac atcaaaaata tgattaaaga catcgatatt gtctatcttg
    79741 cttctcatat cgaagaaact gaaaaaatca aaatctatca attagttact aaaaatgaaa
    79801 aaaaattgtt tttgaataca acatttgaaa acttgacatt gatcaatcca aatattatga
    79861 atttcgaaga tgaaagtatt attgaagcat ctggatttag aatccaacct gaatatgaac
    79921 tattcaaacg tttgtttgat atcatcgttt cacttgtttt attagttatt gcttcaccat
    79981 ttatgttatt cacagcaatt ttagtcaaag tgacatcacc gggaccaatc atctataaac
    80041 aagtgcggat cacaaaaaat caaaaggaat tttcaatcta taaattccgc tcgatgaccg
    80101 ccacagccga agcaaaatct ggaccagttt tagctaagag caatgatgca cgagtaacgc
    80161 ctgtaggtaa atttattcga gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc
    80221 tgaaaggcga tatgtcgatc gtaggaccac gtcctgaacg ttctttcttc gtcgatcagt
    80281 tcaatgcaga aaacccttat tactacttac ggcataatgt tcgtgctggt atcactgggt
    80341 atgcacaagt ctatggaaaa tatgtctctg attacaacag taaattacgt tttgatttac
    80401 tttatatcaa aaaatattca ttgatgcttg atttaaagat cttgctgcaa accatcaaga
    80461 tcctatttga taaaatgtcc tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg
    80521 cagaaattgc ttttccgaat gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca
    80581 atccgaaatt cagcataatc gtaccagtat acaatgcaga agcaactatt ggtcgaacga
    80641 ttgatattct aaaagaattg gattatgaag agtacgaagt cgttttagta aatgatggat
    80701 caacagatca aacacaggaa atcattcaag aactgattga aaatgatgaa cgttttgaat
    80761 tgattactac gaccaatcaa gggcccggat tagcaagaaa cgaaggaata agacagtcaa
    80821 aaggagaata tctcttattc ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag
    80881 attatgatca tttgctagaa aagagtccaa aagcggatct tgttgttagt tcgtttgttt
    80941 ttcgtacaat ggacgaggga agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc
    81001 actatctaac tcacgaagag tttataaaag acatgtatca gttaatgaat gaacagttga
    81061 tgtatgttgt atggaacaaa tgctatcgta gcgatattct gaaaaataac caaattctat
    81121 ttaaaactta taatagttgt gaggatcgaa tatttaactt acattattat gaatactgtc
    81181 atcaagtgat catgaaccca aaagtagaat atatttatga atttgaaggt gggaagggga
    81241 tcactaatca ataccgacct aacaagttcg aaacatttaa agaattctat tcattagcaa
    81301 atactgtaac agacgatatt aataaagacg gtatggcggc gcttctttta aaagggacaa
    81361 cttcagttat tttttctatt tatagtacct ctgcaagaac ttcaaaagag aagaaagacg
    81421 aagcaaagca gatattgagt gatccagtga tccgagaagc caagcaaatt gctaagacgg
    81481 attcttcagc aaaaaaaata acaaagttgc tttacaatat gccagctcca ctatttttat
    81541 ctgctgtaaa agttggaagc ttcgttgaag tgaaaatgcc agggttaatg gctgctttga
    81601 agcgagtata ttagttaatg gaggaaaata taatgaaaag aattttgcat tttcagggac
    81661 gaatgggttt aggtggagca gagtctttca tgatgaattt atatcgtaaa attgatcgta
    81721 caaactatca atttgatttt ttgatttatg aagattatgc agatgttcaa gactatcatt
    81781 cagagattga aagattagga ggccgtattt ttgttgtacc taatcctaag aaaaatattt
    81841 ttaaatattt aatagaagtg aataagttac taaaaaaaga atctttttct atagttcaca
    81901 atcaggttta ttttggcggg ggaattaatt tatggttagc caaaaagaat ggaatcagac
    81961 agagaattgc tcatagccat gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg
    82021 tattgcgtaa atttttaacc aaattgttgt tacaaaatgc aacagactac cttgccgtta
    82081 gtcaagaagc tggtgaaagt ctgtttcaaa accatccctt tgagatagtt cataatggta
    82141 ttgatttaga gctttatagt aaaaattcag aagctaaggt taataagaga aaagaattgg
    82201 atatctcaat gagtactttt gttgtgggaa acattggacg attagagaaa caaaaaaatc
    82261 aatcttattt gttagagatt tttttagaaa tactaaaaat gaattctgat tcttacttga
    82321 ttttagtagg tgaagggtca cttagaaata aactgaaagc caaagcagta gaattggata
    82381 tagagaaaag agtaaaattt ttaggagaaa gaaacgatat tcccgagtta ttatctgtga
    82441 tggatgtttt tgtaatgcct tctctttatg aaggtctgcc aatggttgga ttagaagcac
    82501 aagcagcaaa attaaaatta gtcttagcag atactatttc caaagataca aagttaacaa
    82561 ataatgtgga ttttgtatca ttagatgaaa caccacaaga atgggcaaaa aaagtaataa
    82621 aagagccgta tgctaataaa ataacacaag aactaaagag ttatgatgtt tcgtacacta
    82681 ctgaacaaat ggagagtata tatagtagag atcattaaaa atgttcaaga tattttagga
    82741 gtgattattt ttgaaaatat gttatttgat attggcatac aa
//
LOCUS       DAJSBN010000005        80436 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJSBN010000005 DAJSBN010000000
VERSION     DAJSBN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.005"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..235
                     /locus_tag="ONT86_000373"
     CDS             <1..235
                     /locus_tag="ONT86_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR2351193.1"
                     /translation="SGKSLEALELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVI
                     TNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            470..706
                     /locus_tag="ONT86_000374"
     CDS             470..706
                     /locus_tag="ONT86_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351194.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            713..2665
                     /locus_tag="ONT86_000375"
     CDS             713..2665
                     /locus_tag="ONT86_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR2351195.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2737..3234)
                     /gene="dfrG"
                     /locus_tag="ONT86_000376"
     CDS             complement(2737..3234)
                     /gene="dfrG"
                     /locus_tag="ONT86_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR2351196.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3596..4636
                     /locus_tag="ONT86_000377"
     CDS             3596..4636
                     /locus_tag="ONT86_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2351197.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4734..4922
                     /locus_tag="ONT86_000378"
     CDS             4734..4922
                     /locus_tag="ONT86_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2351198.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4980..5333)
                     /locus_tag="ONT86_000379"
     CDS             complement(4980..5333)
                     /locus_tag="ONT86_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2351199.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5864..6286
                     /locus_tag="ONT86_000380"
     CDS             5864..6286
                     /locus_tag="ONT86_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2351200.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6283..6513
                     /locus_tag="ONT86_000381"
     CDS             6283..6513
                     /locus_tag="ONT86_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2351201.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            7011..7211
                     /locus_tag="ONT86_000382"
     CDS             7011..7211
                     /locus_tag="ONT86_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR2351202.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7238..8431
                     /locus_tag="ONT86_000383"
     CDS             7238..8431
                     /locus_tag="ONT86_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2351203.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8499..10064)
                     /gene="guaA"
                     /locus_tag="ONT86_000384"
     CDS             complement(8499..10064)
                     /gene="guaA"
                     /locus_tag="ONT86_000384"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR2351204.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10123..11046)
                     /locus_tag="ONT86_000385"
     CDS             complement(10123..11046)
                     /locus_tag="ONT86_000385"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR2351205.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11242..11847
                     /locus_tag="ONT86_000386"
     CDS             11242..11847
                     /locus_tag="ONT86_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2351206.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12155..13171
                     /locus_tag="ONT86_000387"
     CDS             12155..13171
                     /locus_tag="ONT86_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR2351207.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13312..14613
                     /locus_tag="ONT86_000388"
     CDS             13312..14613
                     /locus_tag="ONT86_000388"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCR2351208.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14659..15321
                     /gene="deoC"
                     /locus_tag="ONT86_000389"
     CDS             14659..15321
                     /gene="deoC"
                     /locus_tag="ONT86_000389"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR2351209.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15342..15740
                     /locus_tag="ONT86_000390"
     CDS             15342..15740
                     /locus_tag="ONT86_000390"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR2351210.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15806..16888
                     /locus_tag="ONT86_000391"
     CDS             15806..16888
                     /locus_tag="ONT86_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2351211.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17100..18662
                     /locus_tag="ONT86_000392"
     CDS             17100..18662
                     /locus_tag="ONT86_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2351212.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18655..19788
                     /locus_tag="ONT86_000393"
     CDS             18655..19788
                     /locus_tag="ONT86_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2351213.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19788..20744
                     /locus_tag="ONT86_000394"
     CDS             19788..20744
                     /locus_tag="ONT86_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2351214.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20843..22009
                     /gene="deoB"
                     /locus_tag="ONT86_000395"
     CDS             20843..22009
                     /gene="deoB"
                     /locus_tag="ONT86_000395"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR2351215.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            22033..22851
                     /locus_tag="ONT86_000396"
     CDS             22033..22851
                     /locus_tag="ONT86_000396"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR2351216.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22868..23575
                     /gene="deoD"
                     /locus_tag="ONT86_000397"
     CDS             22868..23575
                     /gene="deoD"
                     /locus_tag="ONT86_000397"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR2351217.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23873..24559)
                     /gene="gpmA"
                     /locus_tag="ONT86_000398"
     CDS             complement(23873..24559)
                     /gene="gpmA"
                     /locus_tag="ONT86_000398"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR2351218.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24731..25312)
                     /locus_tag="ONT86_000399"
     CDS             complement(24731..25312)
                     /locus_tag="ONT86_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2351219.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25445..26128)
                     /gene="rpiA"
                     /locus_tag="ONT86_000400"
     CDS             complement(25445..26128)
                     /gene="rpiA"
                     /locus_tag="ONT86_000400"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR2351220.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26447..26860
                     /gene="rpsL"
                     /locus_tag="ONT86_000401"
     CDS             26447..26860
                     /gene="rpsL"
                     /locus_tag="ONT86_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR2351221.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26944..27414
                     /gene="rpsG"
                     /locus_tag="ONT86_000402"
     CDS             26944..27414
                     /gene="rpsG"
                     /locus_tag="ONT86_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR2351222.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27508..29595
                     /gene="fusA"
                     /locus_tag="ONT86_000403"
     CDS             27508..29595
                     /gene="fusA"
                     /locus_tag="ONT86_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2351223.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29767..30954
                     /gene="tuf"
                     /locus_tag="ONT86_000404"
     CDS             29767..30954
                     /gene="tuf"
                     /locus_tag="ONT86_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR2351224.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31361..31669
                     /gene="rpsJ"
                     /locus_tag="ONT86_000405"
     CDS             31361..31669
                     /gene="rpsJ"
                     /locus_tag="ONT86_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR2351225.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31700..32329
                     /gene="rplC"
                     /locus_tag="ONT86_000406"
     CDS             31700..32329
                     /gene="rplC"
                     /locus_tag="ONT86_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR2351226.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32357..32980
                     /gene="rplD"
                     /locus_tag="ONT86_000407"
     CDS             32357..32980
                     /gene="rplD"
                     /locus_tag="ONT86_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR2351227.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32980..33270
                     /gene="rplW"
                     /locus_tag="ONT86_000408"
     CDS             32980..33270
                     /gene="rplW"
                     /locus_tag="ONT86_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR2351228.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            33309..34142
                     /gene="rplB"
                     /locus_tag="ONT86_000409"
     CDS             33309..34142
                     /gene="rplB"
                     /locus_tag="ONT86_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR2351229.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            34185..34463
                     /gene="rpsS"
                     /locus_tag="ONT86_000410"
     CDS             34185..34463
                     /gene="rpsS"
                     /locus_tag="ONT86_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR2351230.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34486..34833
                     /gene="rplV"
                     /locus_tag="ONT86_000411"
     CDS             34486..34833
                     /gene="rplV"
                     /locus_tag="ONT86_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR2351231.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34847..35503
                     /gene="rpsC"
                     /locus_tag="ONT86_000412"
     CDS             34847..35503
                     /gene="rpsC"
                     /locus_tag="ONT86_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR2351232.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35506..35940
                     /gene="rplP"
                     /locus_tag="ONT86_000413"
     CDS             35506..35940
                     /gene="rplP"
                     /locus_tag="ONT86_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR2351233.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35930..36118
                     /gene="rpmC"
                     /locus_tag="ONT86_000414"
     CDS             35930..36118
                     /gene="rpmC"
                     /locus_tag="ONT86_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR2351234.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            36143..36409
                     /gene="rpsQ"
                     /locus_tag="ONT86_000415"
     CDS             36143..36409
                     /gene="rpsQ"
                     /locus_tag="ONT86_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR2351235.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36467..36835
                     /gene="rplN"
                     /locus_tag="ONT86_000416"
     CDS             36467..36835
                     /gene="rplN"
                     /locus_tag="ONT86_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR2351236.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36872..37180
                     /gene="rplX"
                     /locus_tag="ONT86_000417"
     CDS             36872..37180
                     /gene="rplX"
                     /locus_tag="ONT86_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR2351237.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            37208..37747
                     /gene="rplE"
                     /locus_tag="ONT86_000418"
     CDS             37208..37747
                     /gene="rplE"
                     /locus_tag="ONT86_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR2351238.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37766..37951
                     /locus_tag="ONT86_000419"
     CDS             37766..37951
                     /locus_tag="ONT86_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCR2351239.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37987..38385
                     /gene="rpsH"
                     /locus_tag="ONT86_000420"
     CDS             37987..38385
                     /gene="rpsH"
                     /locus_tag="ONT86_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR2351240.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38417..38953
                     /gene="rplF"
                     /locus_tag="ONT86_000421"
     CDS             38417..38953
                     /gene="rplF"
                     /locus_tag="ONT86_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR2351241.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            39114..39470
                     /gene="rplR"
                     /locus_tag="ONT86_000422"
     CDS             39114..39470
                     /gene="rplR"
                     /locus_tag="ONT86_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR2351242.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39491..39991
                     /gene="rpsE"
                     /locus_tag="ONT86_000423"
     CDS             39491..39991
                     /gene="rpsE"
                     /locus_tag="ONT86_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR2351243.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            40006..40185
                     /gene="rpmD"
                     /locus_tag="ONT86_000424"
     CDS             40006..40185
                     /gene="rpmD"
                     /locus_tag="ONT86_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR2351244.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            40229..40669
                     /gene="rplO"
                     /locus_tag="ONT86_000425"
     CDS             40229..40669
                     /gene="rplO"
                     /locus_tag="ONT86_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR2351245.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40669..41964
                     /gene="secY"
                     /locus_tag="ONT86_000426"
     CDS             40669..41964
                     /gene="secY"
                     /locus_tag="ONT86_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR2351246.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            42026..42673
                     /locus_tag="ONT86_000427"
     CDS             42026..42673
                     /locus_tag="ONT86_000427"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR2351247.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42864..43082
                     /gene="infA"
                     /locus_tag="ONT86_000428"
     CDS             42864..43082
                     /gene="infA"
                     /locus_tag="ONT86_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR2351248.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            43115..43231
                     /gene="rpmJ"
                     /locus_tag="ONT86_000429"
     CDS             43115..43231
                     /gene="rpmJ"
                     /locus_tag="ONT86_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR2351249.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            43249..43614
                     /gene="rpsM"
                     /locus_tag="ONT86_000430"
     CDS             43249..43614
                     /gene="rpsM"
                     /locus_tag="ONT86_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR2351250.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43642..44031
                     /gene="rpsK"
                     /locus_tag="ONT86_000431"
     CDS             43642..44031
                     /gene="rpsK"
                     /locus_tag="ONT86_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR2351251.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            44111..45049
                     /locus_tag="ONT86_000432"
     CDS             44111..45049
                     /locus_tag="ONT86_000432"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR2351252.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            45077..45457
                     /gene="rplQ"
                     /locus_tag="ONT86_000433"
     CDS             45077..45457
                     /gene="rplQ"
                     /locus_tag="ONT86_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR2351253.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45729..46568
                     /locus_tag="ONT86_000434"
     CDS             45729..46568
                     /locus_tag="ONT86_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2351254.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46544..47413
                     /locus_tag="ONT86_000435"
     CDS             46544..47413
                     /locus_tag="ONT86_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2351255.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47403..48200
                     /locus_tag="ONT86_000436"
     CDS             47403..48200
                     /locus_tag="ONT86_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR2351256.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48403..49149
                     /gene="truA"
                     /locus_tag="ONT86_000437"
     CDS             48403..49149
                     /gene="truA"
                     /locus_tag="ONT86_000437"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR2351257.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49482..52151)
                     /locus_tag="ONT86_000438"
     CDS             complement(49482..52151)
                     /locus_tag="ONT86_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCR2351258.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(52389..52577)
                     /locus_tag="ONT86_000439"
     CDS             complement(52389..52577)
                     /locus_tag="ONT86_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR2351259.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            52847..53209
                     /locus_tag="ONT86_000440"
     CDS             52847..53209
                     /locus_tag="ONT86_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2351260.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            53261..54943
                     /locus_tag="ONT86_000441"
     CDS             53261..54943
                     /locus_tag="ONT86_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCR2351261.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            55060..57096
                     /gene="recG"
                     /locus_tag="ONT86_000442"
     CDS             55060..57096
                     /gene="recG"
                     /locus_tag="ONT86_000442"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR2351262.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            57145..58146
                     /gene="plsX"
                     /locus_tag="ONT86_000443"
     CDS             57145..58146
                     /gene="plsX"
                     /locus_tag="ONT86_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR2351263.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            58268..58510
                     /gene="acpP"
                     /locus_tag="ONT86_000444"
     CDS             58268..58510
                     /gene="acpP"
                     /locus_tag="ONT86_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR2351264.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            58778..59782
                     /locus_tag="ONT86_000445"
     CDS             58778..59782
                     /locus_tag="ONT86_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2351265.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            59783..60727
                     /locus_tag="ONT86_000446"
     CDS             59783..60727
                     /locus_tag="ONT86_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2351266.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            60727..61689
                     /locus_tag="ONT86_000447"
     CDS             60727..61689
                     /locus_tag="ONT86_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2351267.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            61716..62630
                     /locus_tag="ONT86_000448"
     CDS             61716..62630
                     /locus_tag="ONT86_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2351268.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            62656..64437
                     /locus_tag="ONT86_000449"
     CDS             62656..64437
                     /locus_tag="ONT86_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2351269.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            64785..65471
                     /locus_tag="ONT86_000450"
     CDS             64785..65471
                     /locus_tag="ONT86_000450"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR2351270.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            65491..69072
                     /gene="smc"
                     /locus_tag="ONT86_000451"
     CDS             65491..69072
                     /gene="smc"
                     /locus_tag="ONT86_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCR2351271.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            69081..69890
                     /locus_tag="ONT86_000452"
     CDS             69081..69890
                     /locus_tag="ONT86_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2351272.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            69901..70899
                     /gene="ftsY"
                     /locus_tag="ONT86_000453"
     CDS             69901..70899
                     /gene="ftsY"
                     /locus_tag="ONT86_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR2351273.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            71086..73353
                     /gene="gshAB"
                     /locus_tag="ONT86_000454"
     CDS             71086..73353
                     /gene="gshAB"
                     /locus_tag="ONT86_000454"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCR2351274.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(73442..73894)
                     /locus_tag="ONT86_000455"
     CDS             complement(73442..73894)
                     /locus_tag="ONT86_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCR2351275.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            74044..74994
                     /locus_tag="ONT86_000456"
     CDS             74044..74994
                     /locus_tag="ONT86_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR2351276.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            74987..75946
                     /locus_tag="ONT86_000457"
     CDS             74987..75946
                     /locus_tag="ONT86_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR2351277.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            75943..76698
                     /locus_tag="ONT86_000458"
     CDS             75943..76698
                     /locus_tag="ONT86_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2351278.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            76721..77674
                     /locus_tag="ONT86_000459"
     CDS             76721..77674
                     /locus_tag="ONT86_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2351279.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(78318..78614)
                     /locus_tag="ONT86_000460"
     CDS             complement(78318..78614)
                     /locus_tag="ONT86_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HCR2351280.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            78969..79325
                     /locus_tag="ONT86_000461"
     CDS             78969..79325
                     /locus_tag="ONT86_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2351281.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            79335..80234
                     /locus_tag="ONT86_000462"
     CDS             79335..80234
                     /locus_tag="ONT86_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR2351282.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        26317 a        14464 c        17098 g        22557 t
ORIGIN      
        1 gtccggtaaa tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa
       61 ttgttctctg ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct
      121 tatagaagtt attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg
      181 cggaaatgtt ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat attaagtagt
      241 tcagttttgg agtacaaaaa gtgaaaatgt aaacctaaat gagtatcaaa ttgcaactga
      301 atttgatact cgttttttat tgttaaatca acgtttgttg ttaatttaca ccacaaaatg
      361 taagttaaaa tgtatataaa aatgcgaacg tgaaataatt attgtaggaa tgcaaattgg
      421 tgtgttaaaa tggttacaat ttactaagca aaaaggggtg cattttccta tgcaatttct
      481 tgagccaaaa aataagaacg ctaagtctgt ggattgggaa atatcagagc aagtaagggt
      541 gatagttaag caatacgctg aatatgcaga gcgtactgaa agcgaagcag tggatgaatt
      601 cttattgaac attttagacg ataagaagtt tattgaatgg attgccaaca agcgaagtaa
      661 taagcggatt gttgaaaaaa tgggtatcaa agatagagtg ggttgataga caatgaccaa
      721 gcttaaaaga ttagccacga aggaagatga aattgtagat gttaaaattc cgatttcaaa
      781 cgaagaatta aaagataggg ctaaacaata tgatttatta actccaaaac gatttgcaac
      841 aaggtataac aaaatgttgt tcttgccaac gtcttttaaa tggaacggtt ctgaatatcc
      901 cattcaatat aattactgca tcaatccgtt ttgttgtaat tttgggaaag aacagcacaa
      961 attcaaagat gtaaaaggga aaccaagtcg atataaaatg actggtagtt ccaaagataa
     1021 aggtcataaa ggtatgtact gcaatgacaa tcctattggc agaggtgtat cgcaaaactg
     1081 tacagtaacc cctttatcta attggtctgt tgttgaagaa attaaacgcc ttatagaaat
     1141 caatagcatc caagatgttg agccagatta tcaatttcat aaagaaggct gctcggaaga
     1201 agaatcaacc ccattcaatg agccgaagca attttacaaa agggggaaaa gtaggggcaa
     1261 atctcaacgc tatcaatgta aggcgtgtaa gaagttcact aatgttctac caaaaagaga
     1321 agaaaccact acttatcacc aacaaaagaa caccattctt ccaatgttcg ctaagatggt
     1381 tgttggaagg gtttcagtca gtcgtacttg tgatatttta ggaattggag ttggaaccta
     1441 ctatcataag ctggagtggc tgtataggcg ttgtttggag tttttagaac gatatgagac
     1501 acaacccctt caaacaaaga aatttaacga gatgtggctt aatacggaca agatgcacta
     1561 ctacctaaat aacgtccgta agaaaggaca aggttcaaag aaatatacgg ggtttgaaga
     1621 ccttaatatg caaacgtata tagttgtatc tgctgaggtc ttatcaagat atgtgttccg
     1681 ttctgatgtt gcctatgatt ggaatatttc aatggatgaa ttaaatgaag atacaagaaa
     1741 attcaaagaa gaccatctca atacttttag tcgtaaaaat gataggttag attggtctta
     1801 ttatccacaa gaaccatccg ctaacgatag cgaaaatagg aatgcttatc ttcacgaatt
     1861 agggaaaatt actaatagga gtagatttgt cgatggttta aatgttgatg ctccttatac
     1921 tacaactgct cactattggt taattaaaca aatggttaat gccgacgaat ggcgaatgat
     1981 tagtgatgat gacttctcta ttagaaacgc cttttataga gtattcacga aagaattaag
     2041 gttgagcgat gcacatcatt ttatttgcca agttaacaag acgaaaagca gaaaacaatg
     2101 cttaaaagaa ttcggacaag caaaagcaga actgctcgat tggggagata tcagaggatt
     2161 caaaacaaag tttctacgga ctttggcatc tcattatcta actgaattac taacaagtca
     2221 tcaattccac gaagaggcta ttagtaaaga tggagaaaga tatcgcaaat atgccgataa
     2281 ccctatcaaa catccattag ccactaagga taaaggattt tattcagttg attgtagaac
     2341 ggatttatct gctctggaac caaacgaaat cgcaaagatg ctattaaatg ttaatgacca
     2401 ttcgaccaat agctttattc aacagataag acgttatata tcatctcttg aaagaccatt
     2461 aacaacagct cgtggtgata agaagagcta tatttacgct aacttcaatc cgaagtatgc
     2521 tcaatttgcg attacaattc taagaactta ctacaacttt tgtcgccctt ttaaatcagc
     2581 ggataaaaag gttttgacac ctgctcaaag attagggatt acagataaac aatttgattg
     2641 gaaagatata atttatttca aatgacaaaa agagcttgga gtaatcccaa gctcttgtgc
     2701 tttatcttta ctgttcttct tcaattaacg cacccgttaa ctcaataagt tttttctttc
     2761 atatacatga aaatagtagt tatacggatt tttatcattc tttatccctt tttgggcaaa
     2821 tacctcattc cattcctcat aattcacttc tggaaaaaaa gtatctcctt cgaattcatg
     2881 atgtattttt gtgatgtaca ttttctcaac ataagggaaa aacaaattat aaatctgttc
     2941 tcctccgaaa ataaaaattt cttcttcgtt tttacataac tcaaaaacat cttctattga
     3001 atgaacaatt tcacaaccat taaaggtaaa ccccttatct ctcgtcagaa taatatttct
     3061 tctgtcaggt aaggctcttc cgattgattc aaggttcttc ctacctaata ttatcggatg
     3121 tccctttgta gtatttttaa catattccca gtccttggga atcctccaag gaatgtcatt
     3181 ctctttgcca atcactctat tcttatccat cgcagcaatc aaagaaactt tcatttataa
     3241 cacagccttt ttaactaatt taccatctaa tttcttattt ctatcaaaaa accttttctc
     3301 attatcctgc ccgttagttg aataagcaat tttttctata agttatatta tcataatacc
     3361 acaaaatcac aaattttcat tcaattatca ttttcaattg cattttataa atcattttgt
     3421 atgtaatttg gtatgtaagt taaaatgaaa aaagtagtcc attttgaagt acattttgtc
     3481 attttgtact ccaaaactga actacttact tgcatatata cgcctttata gtggagtact
     3541 acatttacga gattcggtta gagtatcaga aaaagaaaaa ataaaagtta cagaaatgta
     3601 tacttcaata aatggtgaat tatgtaagat tgatagagct tattctggag aaattgttat
     3661 tttgcaaaat gagtttttga agttaaatag tgttcttgga gatacaaaac tattgccaca
     3721 gagaaaaaag attgaaaatc cgcaccctct actacaaaca actgttgaac cgagtaaacc
     3781 tgaacagaga gaaatgttgc ttgatgccct tttggaaatc tcagatagtg atccgcttct
     3841 acgatattac gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca
     3901 aatggaagtg attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga
     3961 gcctacagtc atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga
     4021 agtgccgcca aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg
     4081 aagtggaatg cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa
     4141 tgcagttatg gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac
     4201 ggattgtaaa atctgtttta agtacggttt atactatagc cctgttagta ctccagcaga
     4261 ttttcggatg cttactccta ttgtactgga gcaagccttt agaaaagctg gaacagaatt
     4321 gttagagcca tatcttagtt ttaaagttta tgcaccacag gaatatcttt cacgggcata
     4381 taacgatgct cccaaatatt gtgcaaatat cgtaaatact caactgaaaa ataatgaggt
     4441 cattattatt ggagaaattc ctgctcgatg tattcaagat tatcgcaatg atttaacttt
     4501 ttttacaaat gggcttagtg tttgtttagc agagctaaaa ggatatcagg ttaccactgg
     4561 cgaacctgtt tgccagaccc gtcgtctaaa tagtcggata gataaagtaa gatatatgtt
     4621 caataaaata acttagtgcg ttttatgttg ttatataaat atggtttctt attaaataag
     4681 atgaaatatt ctttaatata gatttgaatt aaagtggaaa ggaggagatt gttattataa
     4741 actacaagtg gatattgtgt cctatttgtg gaaataaaac aagactacga atacgagtgg
     4801 atactatact taaaaatttc cctttatact gccccaaatg taagaacgaa actttaatta
     4861 atgttcaaaa aatgaatata ataacaatca aagagccaga cgccaagacg cagagccgat
     4921 aatttgagaa atgaaactct catcttatcg gctctttttg tttatctgaa ttttactgac
     4981 tagccttcaa tatttcttgc ttcattgata ccttttgcta gtgcttccat taaggatagt
     5041 tctttgtctg taaagctatc catgtatttt tctacctgta accttcgagt gcttttaatc
     5101 aaattatctg taggtaagaa aaattcatca atagatacat ggagtaatga tacaagatca
     5161 tacagcactt gtgtacttgg gtgttgtcct ttgttttcta tatttgttaa ataacgtggg
     5221 tcaatttcaa tcattgttcc cacttgttca cgagtgagac cccgtttcat tcgagcctct
     5281 ttaatcgcta aaccaacggc tctaaaatcg tatttatctt cttttttacg catactaaat
     5341 cacctctata cattttactg ttcctatata accataaaca ggtatagaaa aacatgttat
     5401 atagtttatg agttcctata gaacactgtg gaatttatta atgcgatgaa tccaatttca
     5461 aatagaaagg tcgctgattt tatttaccta tacaacttta taaaatttag tcatactcct
     5521 agaaagtaga taaaattggc gacttaatgg agggaaaagt cgccatgaga aagataatct
     5581 gtcatctagt tctcttaatt aagttcttat caaaaaaact tatccacctg tactggatag
     5641 atatgaccat caatgtggtt ttgcagatta catagataga aaaatcaaag catatgaaca
     5701 gagaaaacaa tctgtttatg tgcttttttg gtcacttaat aaatatcttt taggatttct
     5761 cttcatttat gcaacaaatc ccccgttcgt gtcggggttt agtatgaagg gagataaaga
     5821 agcacatgcc actccctttc acgacagaaa gggggtgaaa agcatgaaac catcttcttt
     5881 tcaagaaacc atagaaaatc agtttgacta tatctgcaaa caagttattg aagatgaacg
     5941 aaaagattac ttcaaacatc tagacagact atcagcaaaa gaagtctcgt tttctgatat
     6001 agataactac cttgtcaact ctttttctac tgttgatacg tacgtcactg atttccaact
     6061 atttaaatta tttggattaa gaatcaatgt tgaaagtgat ttattaagtg aagccctgcg
     6121 tcatctttca gagaaaaaac ggaacattat tttacttcat tactttatgg atatgagtga
     6181 tgtggagatt gcggagttac taaatttgaa tcgttcgact gtttatcgtc atcgaataag
     6241 tggactagcc atgattaaag aattcatgaa ggagtgtaaa gaatgaaaac agcttatcca
     6301 agggttccct tccccctcat tgtaaaggcg acagatggcg acgtggaagc aattaatcag
     6361 attgttaaac attatcgagg gtatacctca aaacgttcat tacgtcgcat gacagacgaa
     6421 tatggaaatt ctcatatggt tattgatgaa accctacgtg gcagaatgga aacgaaactc
     6481 atctctaaga ttttatcgtt cgaaattaaa tagtcccatt ttttcgtgga agcgtgtcga
     6541 atcgtatcca cgcttcccga tttgggaggt ttgttattcc atcaaagcat attgtgtgtt
     6601 cagtaggttt tgataggcta acaagccaaa cgttctttga caaccaaata aaggcaatcg
     6661 aatacgtttc ggtaagaaaa gagctgatga actaacacgc catgacctac aaaattagtg
     6721 agcgattcat gttagtaatt cgaaagtaat ctttagcagg attgctgcca tgactttctt
     6781 atcgtgataa tgatactccc gtatagccat agtccgagcg ttagaagcgt cacaggcaat
     6841 gggtacggct acatgagaac catgcagaag tgaaattcct gtgagctttg ctagagctgt
     6901 tcgattgcta agatattctt ctcttgtcag tgtattatta taaaactatt tgatataatt
     6961 ataggtgaat attggagttt tcctagccta taattgggaa aggagtaata atgataacgg
     7021 tcacaaaaaa ggatttagtt gagctaggat atggaacttc tttttcagca gacatcataa
     7081 aaaaagcaaa agaattgatg attacaaaag ggcatatcta ctatcaatct cgaaagctag
     7141 atagagtccc tcgtgaagct gttgaagaaa ttctaggaat tcggttttca aatgaaaaca
     7201 ggtctgagta gtttttgcac agtaaggagt aaaaactatg gttaaagacc caataaaaaa
     7261 agcaaagaat ggaacctatt attttcgtgc aaatctaggt tatgattcta aagggaaaaa
     7321 aattcaaaaa tatcgtagtg gattcactac taaaaaagaa gcaagagaag aatattcgaa
     7381 actgctattg atgaaaccag aagaattaag tgagaataag gataaaatga cgtttaaaca
     7441 ttatatcttt gaaattttcc ttccatggta taagacacag gtaaagctca gaacatatga
     7501 aaatcgctta ccaactatta agaagcattt ctcttatttt gataaaatgg ctgtttccga
     7561 tattgagcct attgatgtgc aaaattggca actaaaatta tcaaagaagt gtaagtcttc
     7621 ttatgtaaga gctgttcaag gactattttc agttgctatg gatcgagcaa ttgtccttgg
     7681 gataaccacc acaaatccat ctaagattat tggcaatgtg aaaaagcaaa agtctaaaat
     7741 tgaattttgg acaaaagaag aatttgaaaa agtaatctct ctaatttaca aagaagatta
     7801 ctatcaacac tttttattta tctctctgtg gtttttattt atgactggaa tgcgaattgg
     7861 tgaagctact gccattcaat gggaagatat tgattttgac tcaggagtac taaccatcaa
     7921 caaaacactc tattacaaga atcaatcgaa ttatagattt gttgaaccaa aaacaaaggc
     7981 gagcgttcgt catattgtat tagataaatg cacattaact tatttatccg aatggaagga
     8041 agtacaacaa aatctaattc agacagactt tgtaatgagc tataacggta ttcctaccca
     8101 aaaacataca ctatcatatg ctattgaacg ctatgctaag ctggctggta tccatcgaat
     8161 cagaatccat gggttacggc attctcatgc ttccttgtta atcagtatgg gagaaaatcc
     8221 acttatcatt aaagatcgtc taggacacga agatattgag acgactttag gaacgtatgg
     8281 acatctttat cctaacagta atttcgaggt agctaacaaa ttaaatggag ccatttcctt
     8341 taaagaagca aaacaaaatt atgatacttc accaaagaat caatttactg tagactactt
     8401 gcgaaacaaa gaaatgaaaa aagtgcaata aaagtgcagt aaaaagataa gaagagccag
     8461 aaacccttgg tataagagga tttctagctc tcctacaact attcccactc cactgttgct
     8521 ggtggtttag aagtgatgtc atacacgata cggttgacgt gggctacttc gtttacgata
     8581 cgtacagaga ttttttgcaa gacttcccat gggatacggg caaagtcagc ggtcattccg
     8641 tcgattgaag ttacagcacg gataccgact gtgtagtcgt aagtacgtcc gtcacccata
     8701 acaccgacag agcggatccc tggaagaaca gtgaaatatt gccagatatc acggtcaagt
     8761 ccagcagctg cgatttcttc acgcaagata gcatcagatt cgcgtacgat ttctagcttg
     8821 tcttcagtga tttcgccaag aacacgaata ccaagaccag gaccagggaa tggctgacgc
     8881 caaacgattg aatcaggcat gcctaactga gtccctaatt cacggacttc gtctttgaat
     8941 aatgtgttta atggttcgat caattcaaat tgcatatctt ctggcagtcc acctacatta
     9001 tggtgagatt tgatcgtctg tgctgtttct gtaccagatt cgataacgtc agtatacaac
     9061 gttccttgtg ctaggaaaga gacgccttct tcaccagcaa gttttgttgc ttcgtcatca
     9121 aaaacatata cgaattcatt cccaatgatt ttacgttttt gttctgggtc agatacacct
     9181 ttcaacttgt ctaagaagcg atctcttgca tcgactttga tgatgttcaa gccgaatttt
     9241 ccgcctagac tttccattac ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca
     9301 aagatagatg tcaattggtc accgatcgct ttttgtaaaa gaaccccaac gacactagaa
     9361 tcaacgccac ctgaaagacc aagcaagact tttttgtcac caacttgttc acggattttt
     9421 tctacttgca tatcgataaa gttatccatt gtccagttac cttcacagtg acaaacatca
     9481 aaggcaaagt ggcgcagcaa ttcgattccg tattcggaat gacgtacttc tgcatggaat
     9541 tggataccat aaaaattacg tgttgtgtct tggatagaag caatcggaca atctttgctt
     9601 gttccgactg tttcaaagcc ttcaggaact ttagttacca agtcaccatg gctcatccat
     9661 actgtttgtt gttttggtgt ttctttaaac aagacagctt gatcgcttgt tacttctaac
     9721 aaggctttcc catattcgcg gtttttagca ggctctacgg aaccgccaag tttgtacgtg
     9781 atcaattgca taccatagca aatccctaat acaggaatac ctaaattgaa gatctcagga
     9841 tcgatcccaa atgcattctc atcatataca ctgtttggtc cgccagaaag aatgattccc
     9901 tttggattca tcgcttttac ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag
     9961 actccaagat cacgaatacg tcttgtgatc aattggttat actgactacc gtagtctaat
    10021 acgataatct tttcaacgtt cgtcatatcg gcaacgtttg tcaaatttat tacccctatc
    10081 cctttaaatt ttatctcaaa atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa
    10141 aatcgaatcg atgatatgat tttcagtttt atggagaatt atatcggctc ttcctcgtgt
    10201 aggcaagata tattcctgta aatttttcaa attcacattt ttccatacat cttttgccat
    10261 tttaaaggct tcttcacgat ctccgatagc aaaacgataa taataattct ctggattttg
    10321 gaaagccgta tccagtaacg caccaaaacg atccaaatac catttttcga tcagcacggg
    10381 atcggcatcg acatagacag aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc
    10441 aggtaattga agcgtattga tcccctcaac gatcaagata tcaggctgct ggatcaattc
    10501 gtacttgtct ggaatgatat catacacatc atgtgagtaa acaggtactt ttatctcttc
    10561 ctgaccagat tttacacggt tcaagaaatc gatcagcatc tccatatcat aactttcagg
    10621 aaatccttta cggtccatga ttccacgctc ttttaatata cgatttggat aaagaaatcc
    10681 gtcagtcgtg atcagctgaa cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag
    10741 cagtctggct gttgtacttt ttccaacggc tacacttccg gcgatcccga taataaaagg
    10801 cggtacttct acgtatttat gaagaaataa acctttactc aagaacaaag attcaaattc
    10861 tttcatataa agataaatca gatgagcaag aggtacatag atatctcgaa catcctgcat
    10921 ggaaatgcgg tcattcaaac ttttgatatt atctagttca tcttgtgtca aaggtgcttg
    10981 cccattacga taaaattctc gccattcttc tcttgccaca cggtagtagt tcattggatc
    11041 attcatatta gcctcctgcg ttctcccatc aatggaacta ttttaacaca aaataaacgc
    11101 aaaccccacc acttaagaga gattgccgat ctttttgtat aatctttata atagaaagaa
    11161 acggagtctt attcttgact tgcgcacgga tacataaaag gatagactgt tgtcatgaaa
    11221 gaaatgagaa ggatggaaga catgacaaat cattactatt ctgaacgccc agatacggcg
    11281 cacgattttg aacaatgggc ttttgagtta aaaggcagaa cctttcaatt tgtaacagat
    11341 tctggtgtat tttcacgtga aacagtggat tatggttcac gtgtcctgat tgatgctttc
    11401 gattgggaag agttacctga tgaaggaaag atcttagatg tgggatgcgg gtatggaccg
    11461 attgggttag ctttggcatt tgcgacacag cggtttgttg aaatggtcga cattaatgaa
    11521 cgagcggtaa gtctagcgca aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc
    11581 catcaatcga atatctatga agcagttcat gaagaaacct atgcagctat cgtaagcaat
    11641 ccgccgatcc gtgctggaaa aaaagttgta catgaaattt tgacaggcgc atatcctcgg
    11701 ttgaagaagg gcggtacact gacaattgtg atccagaaga aacaaggagc gccgagtgcg
    11761 caaaagaaaa tggaagaaac atttggtaat gcagaaatcg tcacaaaaga taaaggatat
    11821 tatatcatca aaagtgtgaa agaataacta gctgaaaaat agtggagcga cgaaagagtg
    11881 gaactgggcg tgcatgtacg ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa
    11941 acaaagaact attttttctt caaaaagaaa tagtaggaac agagtcgttg ttttttacga
    12001 taaaataaat aagatacaga acttgtatag aaggctgcca agtcggtaga taaaggaggt
    12061 ttttatcaaa aaacatactt tccttttttc tctattcttt tcttgggggt tttggtacac
    12121 tgatagggaa tttagataga agggagccaa taggatgaag ctgaccatca gagatatcgc
    12181 agaaatggca ggcgtatcag taacaactgt ctcacagatt ttgaataaca aaggaagcag
    12241 atttagtgat aagacaagaa aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc
    12301 cgattatttc gcttcaaatg tgatcaatcg acattcaaag accatcggaa tgatcgtccc
    12361 tgatgtcact gatttcttct tctccaaact gatcgaaggt gtagagagct atttgaatcc
    12421 tttgggctat gtgatcatgt tatgcaattc tcgtcatagc caagaaaatg agatcaagta
    12481 tttacaagag ctttcccacc gctcagtgga cggaatcttg ctggccacac caaatatttt
    12541 acctgagaaa tatgcattag acagcggttt ttatcaaaaa atgccaatca ttttaatcga
    12601 tcgcggattg aatcgtcggg ataacggacg gcttatcgta aaggaatatg agggcgccta
    12661 tcaagctgtt tcgctgttta ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac
    12721 cactggatac tatcaattag aagaacgttt caacggatat cgtcatgcgc tgaaagatca
    12781 tggcctgcct tttaatggga aatttgttga acatggagag ttgactgtcc aaggtggata
    12841 tactgcatca aaaaaattac tcaaacataa agatatcacg gctatttttt gcgggaatga
    12901 tgccatggcg atcggatgct atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga
    12961 tatctcagtc ataggattcg atggattaaa gctgtcagaa tacatgattc cgcaattgac
    13021 gacagtgcag cagccaagtt ttgatattgg attttatgct gcacgtttcc tgatcgatac
    13081 gatcgaattt cctcaaagaa aagttccgaa caaagtgttc gaaacaaaat taattattcg
    13141 tgagagcgta aaagtattga caaagggcta actcggcggt atattgctta tgtaagcatt
    13201 tacaaaggct agttagtgct ttttttattt ttagtaaacc agtttactaa agtggtttac
    13261 ctttcctaaa aataatgtat aataccgaat gaagacaagg aggatcaggc aatgcgaatg
    13321 gtggatctga ttgaaaaaaa gagggatgga aacgagctgt cgaaagagga aatcgagtac
    13381 atcgtcacga actatacgaa tggaaagatt ccagattatc aagtcagtgc attattgatg
    13441 gcaatttttt atcaagacat gacaaatgaa gaaatcacga acttaacatt ggcgattgct
    13501 aattctggtg atgtcatcga tttgagttct cttgaaggaa taaaggtaga taagcattcg
    13561 acaggtggcg ttggtgacac gaccacattg attttagccc ccctagtagc tagtgtcgga
    13621 gtgacagtgg ctaaaatgtc tggacgcggt ctaggttaca caggtggtac actagacaag
    13681 ctagaagcaa ttcccggatt tcagatcgag ctttcggatg aagaatttgt acgcattgtt
    13741 aatgaaagta aagtagcggt aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa
    13801 ttgtatgcct tgcgtgacgt cacagcgacc gttgattctc ttccgctgat tgccagttcg
    13861 attatgagta aaaaaatcgc ggcaggagca gatgcaatcg ttttggatgt gaccactgga
    13921 gacggtgctt tcatgaagaa catcgaagat gcaagacgtc tggcaaagac catgacgagc
    13981 attgggaaac tggctaaccg agaaacagtg gcggttatct cagatatgtc agaacctctt
    14041 ggcgaagcta tcggaaatag tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat
    14101 ggtccagaag atctagtaga aatgtgctac gccttaggaa gccaaatggt tgttcttgct
    14161 ggcaaagcta agacaaccga tgaagcaaga acgttgcttc aagaagctct ggaatcaggt
    14221 aaagcgttag caaaattcaa agaaatgatc caaaatcaag ggggagaccc agcgatcgtg
    14281 gaacatccag aacgaatttt gactgcacgc tacacaatgg agcttcctgc taaacaaagc
    14341 ggagttgtgt caaaaatcgt ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct
    14401 gggcgtaaaa ccaaagaaga agatattgat cacgctgttg gattgaaact gcataaaaaa
    14461 attggtgaca ctgtgacaaa aggtgagtca ctattgacga tctatagtaa cgacgaagaa
    14521 atcacttctg tcattgaatt attatataaa aatattgaaa ttggtgaatc ggcaatgaag
    14581 ccaacgttga ttcatgacat catcacagaa taaaggaaaa aagcggactt taccgtttta
    14641 aagaaaaggg gaaaagaaat ggaactaaac cgaatgatcg accatactat tttgaaagca
    14701 gacgcatcta aagaagctgt gatgcaaatt atcgaagaag caaaaaaata ccatttttat
    14761 tctgtctgca tcaatccagc atgggttgcg ctagcgaaag aacaattaca aggtgagcca
    14821 gtagccgtat gtacagtaat cgggtttcct ctaggagcaa acacacccga gacaaaagca
    14881 tttgaaacaa cagatgccat caataacggt gcagatgaag tcgatatggt catcaatatc
    14941 ggtgctttga aatcaaagga tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa
    15001 gcagcaaaag atcgtgcgct tgtcaaagtt attattgaaa catcgttatt agatagagaa
    15061 gaaatcatcc gagcttgcga aatagcaaaa gctgcaggcg ctgattttgt caaaacatca
    15121 actggtttct caacaggcgg agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta
    15181 ggaccagaaa tgggtgtcaa agcttccggc ggtatccata acgaagaaga agcaatggca
    15241 atgatcgaag ctggtgctac aagaatcgga acaagcgcag gagtagcaat cgtatctggt
    15301 tcaactggtg aaggctatta attttttcaa aggagaagct catggcagaa ctcaaacaag
    15361 aatggatcaa cacagctatt gaagcgttag ataaagccta tgttccttat tcccattttc
    15421 cggtaggcgc ttgtctcgta acggaatcag gcaagattta tcaaggcatc aacattgaga
    15481 atgcatcatt tggattaacg aattgtgccg agcgcacagc ttttttcaaa gcagtatctg
    15541 aaggagaaaa aacatttacg catctagtcg tagcggggca cacccccgat cctatctctc
    15601 cttgtggtgc atgtcgacaa gtaatggcag aattctgttc ccctgatatg ccggttacgc
    15661 tagtaggaga caatggtgtg acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat
    15721 ttactgaaaa agatctataa ctcatgttca ttcagtcagc tgactgttga aaaataaaac
    15781 aattatttta ggaggcttta cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt
    15841 cgcactagca gcagggttat ttttaggtgc atgcggcaac aacggctcta ctgattcatc
    15901 cggtggcaaa agctcatcag acacaacaac tgcagcattg atcacagaca caggtggtgt
    15961 agacgaccgt tcattcaacc agtcagcttg ggaaggacta gaaaaatggg ggaaagatca
    16021 aggactttca cgaggaaatg atggcttcca atatttccaa tcttcaaatg aatcagatta
    16081 catcccaaat attgaccaag cattgaatgc tggattcaaa acgatttttg gtattggtta
    16141 taaactaaaa ccagcgatcc aagaacaagc aacaaacaat acaggtacga actttgttat
    16201 tatcgatgat gtcatcgatg gattagataa cgttgtatct gcaactttca aagacaacga
    16261 agcttcttat cttgcaggtg ttgcagcagc ttatacgact gaaacaaacg tagtcggctt
    16321 tatcggcggt gtaaaaggtg aagtaatcga tcgttttgat gctggattca aagccggtgt
    16381 tgatgcaggt gcgaaagaat taggaaaaga aatcaaagta ttgaatcaat atgctggtga
    16441 cttctctgct ccagataaag gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga
    16501 tatcatcttc catgcttcag gcggtacagg taacggtgta ttccaagaag caaaatcatt
    16561 gaacgaatca ggcgacaaaa aagtttgggt tatcggtgtt gaccgcgacc aatcagacga
    16621 aggcgaatac acattaaacg gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc
    16681 agttggaaca gtagtagaag atttggcaca aaaatcagcg gacggtaaat tccctggcgg
    16741 cgaacacact gtttatggat tgaaagaaga cggtgttggt ctaacagaag gacaactttc
    16801 tgacgaagcg aaaaaagcag ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa
    16861 agttccagaa actcctgaag aaaactaaaa ccatttctat agccttgtta gacacatgtt
    16921 tgacaaggct atggtttgat tagaacaaga attgttcatt tatccaagct gccttgtgac
    16981 agtttggata agggaacaac tccctgaagt aatgtttgtt tcaaaagaag aacgctcact
    17041 aagcgtgaat cttagtcaat cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg
    17101 tggttcaaga aaatattgtc atagaaatgc agaatattac caaagaattt ggaacattca
    17161 aggcaaatga caacatcaat ttacaagtaa aggcaggaga aattcatgcg ttgcttggag
    17221 aaaacggtgc tggcaaatct acattgatga acgtgctttc cggattatta gagccgacat
    17281 cagggaaaat tttgatgcgt gggaaagaag taaagatcac aagtccgacc aaagccaacc
    17341 aattagggat tgggatggtc catcagcact ttatgcttgt tgatgccttt actgtaacag
    17401 aaaacatcgt gttgggaagc gagcctagtc gcgcagggat gcttgaccat aaaaaagcac
    17461 gaaaagagat ccaaaaagtt tctgaacagt atggattgtc agtcaaccca gatgcttacg
    17521 ttcgtgatat ttcagttgga atggaacaac gggtagaaat tttaaaaaca ctttaccgag
    17581 gagcagatgt actgattttt gatgagccga cagctgtatt gacccctcaa gaaattgatg
    17641 aattaatcgt gatcatgaag gaattagtca aagaaggcaa gtcgatcatt ttgattacgc
    17701 ataagttaga tgaaatcaaa gcagtagctg accgctgtac agttatccgc cgtggaaaag
    17761 gaatcggtac agtcaacgtt aaagacgtaa cctcacaaca attagccgat atgatggttg
    17821 gaagaacggt ttcattcaaa acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt
    17881 ctattgaaaa tctagtggtg aaagaaaatc gtggattaga agccgtgaaa aacttgaact
    17941 tagaggttcg tgctggcgaa gtacttggta tcgctggaat cgatggaaac gggcagtcgg
    18001 agttgatcca agctttgact ggtttgcgaa aggcagaaag cggacatatc aagctaaaag
    18061 gggaagacat cactaataaa aaaccccgaa aaatcactga acatggtgta ggacacgtgc
    18121 cagaagaccg tcataaatac gggttagtcc tagatatgac attatctgaa aatatcgcac
    18181 tacaaaccta tcatcaaaaa ccttacagta aaaacggcat gctgaattat tcagtaatga
    18241 atgaacatgc cagagaattg atcgaagaat atgatgttcg aacaacgaat gaacttgttc
    18301 ccgcaaaagc tttatcaggc ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc
    18361 gagatcctga tctgttgatt gttgcaaatc caactcgtgg actggatgta ggtgcgatcg
    18421 aatttattca taaacgtctg atcgaacaaa gggacaaata caaagcagtg ttactgatca
    18481 gtttcgaatt agaagaaatt ttaaatgttt cggatcgtat tgctgttatc catgaaggag
    18541 aaattgtcgg tatcgttgat ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg
    18601 cgggctatac cttagaagaa gcacgaaaag agttagaaaa gggggctggc gcacatgaat
    18661 gatcgcagcg aaaaattccg taatttatta gtgcctgtac tttcagttgt tttaggattg
    18721 atcttaggcg cgatattgat ggctgctttt ggttttcagc ccatccaagg ttattcttct
    18781 atgctagatg cagcccttgg ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg
    18841 ccgttgattt ttacagcatt aggtttttct gtagctaact cagctgggtt tttcaatatt
    18901 gggctttctg gacaagcact ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt
    18961 ccagatctgc caaaagttgt tttgcttccg ttatgcgtga tcttaggtgc cttagctggt
    19021 gcgatggcag cggctgttcc aggtttactt cgagcatttt ttggaacaag tgaagtgatc
    19081 gtaacaatca tgatgaacta tatcttatta tacgtaagca catttgcttt acatgaagtg
    19141 atgcctgaaa gctatcgctc aagtttggac tcttccaata tgatcagtca aaatgcctca
    19201 ctgcggttac catggttaac acaagctttt ggcggctctc gcgtaaactc aggattattt
    19261 ttagcaatca tcgcgcttat tttcgtttgg ctgctgatga agaaaacgac tttaggattt
    19321 gaaatccgtt ctgttggatt gaatcctttt gcttctgagt atgcaggaat gagtagtaaa
    19381 cgtacgatcg ttttgtcgat ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt
    19441 gaaggattag gaacgtatca aaacttcttt gttcaaacga cttcgctatc tattggattt
    19501 gacggaatgg ctgtttcctt gcttggttct ggatcagcaa tcggtattct tttatcagcg
    19561 atcttgttca gtattttgaa gatcggtgga cttggcatgc agacaggagc aggtgttccg
    19621 tttgagatcg tcaatgtatc gattgctttg attatcttct ttgtcgggat caacttcctg
    19681 attcgtttcg tcatggctaa agtcttccaa gggaaaaaac aagaagagat cgttgcgaca
    19741 atagaatcga aaccaacgaa tcaaaaccat gaagggggcg aactataatg tcgactatcg
    19801 aattaattgc cttgatcctt acttctacac tggtttattc aacaccgcta attctaacgt
    19861 cactaggcgg aactttttct gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca
    19921 tggtcatggg tgcttttagt tccatcgttt tcaacttgac gatgagtcca tcacttggtt
    19981 ctatgacacc atggatcgct attttagtgg gtggcgtcgt aggtatccta ttttctctga
    20041 ttcacgcagt agcaacagtc agtttgcgtg ctgaccatat tatcagcggg acagtaatta
    20101 atctgatggc acctgcttta ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa
    20161 ctgataacat cacgcagaat ctggggtatt tttctttccc aggattagca gatattccgg
    20221 tcattggacc aattttcttt aaaaatacat tcttacctgc ttattgtgct attttgattg
    20281 ctgtcctagc ttgggtgatc atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg
    20341 gtgaaaatcc tcaagctgcc gacacattag gtatcaatgt atatgctatg cgttattcag
    20401 gcgtactgat ttctggcttg ttaggcggta tgggcggtgc tgtttttgct cagtcgattt
    20461 ccggtaactt ttcagctggt acgatcgttg gacaagggtt catctccatg gctgctatga
    20521 tctttggtaa gtggaatcct ttaggggcaa tgggtgcctc attgttcttt ggttttgctc
    20581 aaagcttgag tatcattggc gcacaattac cagttatttc ttcgattccg ccagtattgc
    20641 tgcaaattgc tccatatttg ctgacaatca ttgtgctggt tgttttctta gggaaagctt
    20701 ctggtccaaa agcaaacggg aagaactata tcaaatcaaa ataatctttt agcgaataaa
    20761 aagggtgggt tacgggaagt gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa
    20821 taaaaagaaa gaaggaataa tcatgtttaa acgcgtacat ctgatcgtca tggattcagt
    20881 aggaattgga gaagcaccag atgccgataa gttcggtgat gttggttctg atacattagg
    20941 acacattgcg aaagaagcag gattgaacgt tccacattta gagcagcttg gtttaggaac
    21001 gatccgtcct cttgaaggtg tggaatcagt gaaagatcat caaggctatg cgacgaaatt
    21061 agaagaagtt tctgtaggga aagatacgat gacaggccac tgggaaatca tgggattgaa
    21121 tatcaaaact ccatttcgtg tgtttcctaa tggattccct gatgatctga tcaaacaaat
    21181 cgaagaattt tctggccgta aagtcgtatg taacaaacct tatagcggaa cagctgttat
    21241 tgacgattac ggtgaacacc aaatgaaaac aggcgattta attgtatata cttctgccga
    21301 tcctgtcctt cagatcgcgg cacatgaaga aatcattcct ttagaagaat tatatcggat
    21361 ttgccaattc gttcgtgata taacaaaaga tgatccgtat atgattggcc gaatcattgc
    21421 acgtccgtac gtgggtgaac caggagactt cacacgaaca agcaatcgcc atgattatgc
    21481 gttgaatcca ttcggacaca cagtccttga ttctttgaaa gaagcaggaa aggacgtgat
    21541 tgctgttgga aagatcaacg atattttcaa cggacaagga atcactgaat ctgtccgcac
    21601 gaaaagcaat atggatggcg ttgaccagct gctgaaagtt atgaaaaacg atttttcagg
    21661 aatcagcttt accaatttag tagattttga tgcattatac ggtcatcgtc gggatgtagt
    21721 cggctatgcg catgcgattg aagaatttga cttacgaatt ccagaattgc ttgaagcgat
    21781 ggaagaagac gacttgctaa tgattacagc cgatcacgga aacgatccga cgtttactgg
    21841 taccgatcat acgcgagaat atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca
    21901 aggggcattg cctcaaggat attattcaga tatttcagca acgattgctg agaactttag
    21961 tgtttcttcg actgaaaatg gcgaaagctt cttgaaatta ttaaaataat ctttttaaat
    22021 aggagaaagc aaatgaccaa gttaagcgag atgttacaag aaacgaccca gtttatcctt
    22081 gataaaggag taaaagaaat tgattttggt ttgattctag gttcaggatt aggtgaatta
    22141 gcagatgaaa tcgaagatgc tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt
    22201 tcgacagtag ttgggcatgc aggaaagcta gtttatgggt ctctggcagg caagaaagtg
    22261 ttagccatgc aagggagatt ccactactat gaaggtcatt ccatgcaaac agtgacctat
    22321 ccagtacgtg tgatggccgg actaggggcc cattcagtca ttgtgactaa tgcatgtggt
    22381 ggtgtcaacg aatcatttgt accaggagat ttgatgctga ttaccgatca tatcaatttt
    22441 actggtgaaa atcctttgat cggtccgaat gaagatgcaa tgggccctag atttccagat
    22501 atgagtcaag cttatacgcc tgactatcaa gcaacagcaa aagcagcagc agaaaaacta
    22561 ggtttggatc ttaaagaagg cgtatatatg ggctattctg gaccaactta tgaaacgcct
    22621 gcggagatcc gaatgtctcg gacaatagga gccgatgcag tagggatgtc gactgttcca
    22681 gaagtcatcg tagcagctca cagcggatta aaagtgttag gaatctcttg tattaccaac
    22741 ttagcagcag gtatgcaagc gaatctgagc catgaagaag ttgtagaaac aactcagaga
    22801 gtcaaacaat cttttaaagc attggttaaa gaaacattga cattattgta ataggaggaa
    22861 caataaaatg agtgtgcata ttgaagcgaa agaaggcgag atcgccgata agattttact
    22921 tccgggagat cctttgcgag ctaaatatat tgcggagact tttttggaag atccagtttg
    22981 ttacaatcag gtacgcggta tgttaggctt taccggcaaa tataaaggag aacgtatttc
    23041 tgttcaagga acaggaatgg gaatgccatc agccaccatc tatgcccatg aattgatcca
    23101 gtcgtatggt gtaaagaaat tgattcgagt aggtacatgc ggtgcattgt caaaagacgt
    23161 ccatgttcgt gatttagtac tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa
    23221 aaatttccaa gcctttcatt ttcctccaat cagtgatttc aatctattac tgaaagcata
    23281 tgagatagct aaagaaaagg gctatacggt tcatgtagga aatgttcttt cggaagattc
    23341 tttctacaaa gatgatttga cagagacgtt ccaattagct gaacttggcg ttttaggagt
    23401 ggaaatggaa gcagcggctc tttattactt aggcgcgaaa taccatgtgc agacattaag
    23461 tttgatgacc gttagcgacc atttgatcac cggtgaagaa acaacagctg cagaacgtca
    23521 atctacgttc aatgaaatga tcgaagtagg attggaaaca gcgatagcag aataagaagg
    23581 aaatgaaaaa gctgccctgc attaagtaat aagaacatcc aagcaaaaat agctcctcat
    23641 gtttttgact tggctggact tattattgca aatgtaagct tttgaacacc gtttaccaag
    23701 aggttgtgaa tctgccgttt aactctgagt cacaaggaac aatttcaaaa aacagctttt
    23761 catgtttgat gaatattgtg acttgtgacc gagaagttgg caggagaaaa ccgtttatac
    23821 gaaaaaaggg actgtgacaa atcgtgtcac agtccctttt ttatagtgtt ttttataggt
    23881 agtatttttt agcaactgtt aagtcatcat ttaattcata aacaagtggt tgacctgttg
    23941 gaatttcaag atccatgatg tcttcgtcag agataccttc gatatgttta gctagggcgc
    24001 gtaatgagtt accatgtgca gctactaaaa cagttttgtt gtctagtaat gctggagcaa
    24061 tttcatcttg ccagaatggc aaagcgcgtt ctaatgtcac tttcaagttt tctccaccag
    24121 gaatatcgcg tttgtctaac attgcgtaac gacggtcatt tgctgcagaa ccttcgtcag
    24181 tttcttccat taatggagga agagtatcgt aagaacgacg ccagatgtgc acttgttcgt
    24241 cgccgtattt ttcagctgtt tcttttttgt tcaaaccttg taatttacca tagtgacgtt
    24301 cgtttaagcg ccatgatttg atttgtggta cccaaagttg atctgaatat tctaagatca
    24361 agttacatgt tttgattgca cgagttaaaa cagaagtgta agcaacatca aattcgattc
    24421 cagcttcttt gattttgcgt ccgccttctt ttgcttcttc gattccttct ggtgctagat
    24481 ttacatcagc ccaaccagta aattggttca atgcattcca ttcactaaga ccgtgacgag
    24541 aaaaaactaa ttttggcatg tttagctctc cttttaagat aggtttatat ttctgaaatt
    24601 ttcagttctg ttttattgta caattttttg gaagagattt ccatcgaaaa attagataag
    24661 attctatgag aaaacaagta gagaatataa aaaaacgaac agtgcccacg caactgttcg
    24721 tttttaggta ttagatgcat aattgctcga tttctttttc aggtaaattg atatatttat
    24781 aacatgttcc aactgaaaca ccgcattttg aagaaataaa ttggatcgtt tctttttttt
    24841 cataatagag acgtcggatt tttttgatcg tttttgcatc gatttttggt cttccgccga
    24901 tttttccttt tttacgagct tcatctaatc caactaatgt acgttctttg atcaatgcgc
    24961 actccatgtt tgctaggttt tccattaatt ggaaatagat ttttcccatt tcgcgcgtat
    25021 cgattccttc atcaagacta atcaagtgca gcccgcgatc tttgaataat tgcgtgagtt
    25081 ctgttaattg ccgcgtagat ttgcctaatc gatgaagttg gcaaatgact agtgcatcgc
    25141 cttgttgcat gtcagcgatc actgactcca aatgaacagc agtagaatca ttttgttcca
    25201 cttcatatgt ctcatggaaa atttcttgac aaccaaagtc agataattga tcaacctgag
    25261 ttgttaaatc aacgtcattt atggttgtgc gtgcataacc aataattttc atggtgattc
    25321 ctttctagca gtgattttct aactggatat aacagtaaaa atgtagcata aagtacgtca
    25381 aaaagatagt tttttcgcaa attttccgct gtcttttttt attggatgat caagcagttt
    25441 ttctttattt acgacggatt gcgtcaagaa tctcaacgcc tttttctgag ccgacaacta
    25501 ctttagaaac catgtttagg aaaagcccat gttctactac cccaacaagt tcatctaaat
    25561 aggtagctaa gctttctggc tgttcgatga cttctagatg cagatcaatg atgtaatgac
    25621 caccatctgt ggttaagact tctccgtttt cgtctgtacg caagacgggt tggaatcttt
    25681 tttcttcaaa aatatgaagt agctgttggg agccataagg gatcacttca actgggagtg
    25741 ggaacttccc aagtttatcg actaatttag aagaatcaac gatccaaatc gtttcttttg
    25801 agtacgtagc gactattttc tcgaaaagca aagcagcgcc gccgcctttg atcccttgaa
    25861 aatcagcact gatttcatca gcaccgtcta ttgtcaaatc gactacgggg acttcatcaa
    25921 tactctttaa cggtattcct aatttttccg cttgttcttt tgtcgcattt gaagtagtaa
    25981 ctcctgtgat acgtaaacct tcttcacgca tacggcgacc aatctcttct accatataat
    26041 aggcggttga accggtcccg agacctacga tcatcccgtc ctttacgaat ttcgcggctt
    26101 caatgccagc taattgtttt aaattcatct ttgcgtccac ctttttttag tttgagcatc
    26161 gtgctttcct cactatttta gcgtgattta cggtaaaaag ataccattct tttttattcc
    26221 aataaagctt tttcaaaaaa aattgcatct cttgttgaca tgttttttca ttcgtgatat
    26281 tctaacaaag gtgctttatc tacaggtttc tgtgaagaaa cgtgctgact gtttatcaaa
    26341 gcatatttga taaggatgca gacattgcat ttatttttta tcacaaaaaa agaaccacct
    26401 ggatgtgtgg aactagacgc aaatcgtggg aaggaggaaa caaggaatgc ctacaattaa
    26461 ccaattagta cgtaaacctc gtaaatcaaa ggtggaaaaa tctaattcac ctgctttgaa
    26521 caaaggatat aacagcttta agaaaactca aactaacgta aactcacctc aaaaacgtgg
    26581 ggtagcaact cgtgtgggta caatgacacc taaaaaaccg aactcagctt tacgtaaata
    26641 tgcacgtgtt cgtttgtcaa acttaattga agttactgct tatatcccag ggattggtca
    26701 taacttacaa gaacacagtg ttgtattatt acgcggtggt cgtgtaaaag acttaccagg
    26761 ggtacgttac catatcgttc gcggtgcttt agatactgct ggtgttaacg accgtaaaca
    26821 aagccgttct aaatacggaa ctaaaaaacc taaagcttaa ttttaattat taaacaaatg
    26881 aaacttgctt ctactatata gaagtaatta ctatgaacaa tatttcggaa ggaggagtta
    26941 cggatgccac gtaaaggtcc tgttgcaaaa cgtgatgttt taccagatcc tatttataac
    27001 tcaaaattag taactcgctt gatcaaccgt gtaatggttg acggaaaacg cgggattgca
    27061 gctaacatta tctataatgc ctttgacttg atcaaagaat ctacaggtaa cgatccatta
    27121 gaagtgtttg aacaagcaat gaaaaacgtt caacctgttc ttgaagttaa agcacgtcgt
    27181 gttgggggtt caaactacca agtaccagtt gaagttcgcc ctgaacgccg cgttactcta
    27241 ggtcttcgtt gggtagtaaa ctacgctcgc ttgcgcggtg aacacacaat ggaagaacgc
    27301 ttagcaaaag aaatcatgga tgctgcaaac aacacaggtg cttctgttaa aaaacgtgaa
    27361 gacactcata aaatggctga tgccaaccgt gcgtttgctc actatcgctg gtaagattct
    27421 ctctgtttgg tcggctttta taagtcgacc tcagatagtg tccttatttt tagcaactag
    27481 atagtaaaga gaggagaaac aaggagaatg accagagaat tttctttaca aaacactcgt
    27541 aatatcggta tcatggccca catcgatgct ggtaaaacga ctgctactga acgtatcttg
    27601 tactatactg gtaaaatcca taaaattggt gaaactcatg aaggagcttc acaaatggac
    27661 tggatggaac aagaacaaga acgtggtatt acgatcactt ctgctgctac tacagcacaa
    27721 tggaagggtc accgaatcaa catcatcgac acaccaggac acgtagactt cacagttgaa
    27781 gttgaacgtt cattgcgtgt acttgatggt gctgtaactg ttcttgatgc acaatcaggt
    27841 gttgaacctc aaactgaaac agtttggcgt caagcaacaa cttatggtgt acctcgtatc
    27901 gtatttgcaa acaaaatgga caagatcggt gctgacttct tatattcagt atcaacttta
    27961 catgaccgtt tgcaagcaaa cgctcaccca atccaattac caattggtgc ggaagacgac
    28021 ttcacaggaa ttatcgacct tgtaacaatg aaggctgaaa tgtatacaaa cgatcttgga
    28081 acagagatcg aagaaacaga aattcctgaa gaataccgcg aattagctga agaatggcgc
    28141 gaaaaattgg ttgaagctgt agctgaaact gatgaagaac ttactttgaa atacttagaa
    28201 ggtgaagaaa tcactgaagc tgagttaaaa gaaggtatcc gccgcgcaac agttaacgta
    28261 gaattctacc cagtactatg tggttcagca tttaagaaca aaggggttca attactattg
    28321 gacgctgttc ttgattactt gccatcaccg ttagatatcc cagctatcaa agggattgac
    28381 cctaaaactg atgaagaagt agaacgtcct gcagacgaca gcgctccttt ctcagcttta
    28441 gcatttaaag ttatgactga cccattcgta ggtcgtttaa ctttcttccg tgtgtactct
    28501 ggtgttttac aatcaggttc atacgtacaa aacgcaacaa aaggcaaacg tgaacgtgtt
    28561 ggtcgtatcc tacagatgca tgccaactct cgttcagaaa tctctgaagt ttacgctgga
    28621 gatatcgctg ctgctgttgg tttgaaagac acaactactg gggacacact atgtgatgaa
    28681 aagagtctag ttattcttga atccatggaa ttcccagaac cagttatcca agtcgctatt
    28741 gaacctaaat caaaagctga ccaagataaa atgggtgtgg ctttacaaaa actatctgaa
    28801 gaagatccta ctttccgtgc tgaaacaaac gtggaaacag gcgaaacaat catcgctggt
    28861 atgggtgaac ttcacttaga tatcatcgtt gaccgtatgc gtcgtgaatt caaagttgaa
    28921 gctaacgtag gtgcgcctca agtatcttac cgtgaaactt tccgtgctgg cactcaagcc
    28981 gaaggtaagt ttgtacgtca gtctggtggt aaaggtcaat acggtcacgt atggattgaa
    29041 tttacaccaa atgaagaagg tgctggtttc gaatttgaaa atgcgatcgt cggtggggtt
    29101 gttcctcgtg aatacattcc agctgttgaa actggtttga aagatgctat ggaaaatggt
    29161 gtcttagctg gctatccatt agtagatatc aaagctaaac tttacgatgg ttcataccat
    29221 gatgtcgatt caaatgaaac tgccttccgt gttgctgctt ctatggcatt acgtgcagca
    29281 gctaagaaag ctaatccagt aatccttgaa ccaatcatgg ctgtagaagt tgttatccct
    29341 gaagattatt taggtgatgt tatgggtcac gtaactgctc gtcgtggacg tgttgaaggt
    29401 atggaagctc gcgctggcgg ccaacaagtt gttcgtgcga tggttccact tgctgaaatg
    29461 tttggttatg ctacaactct tcgttctgct actcaaggtc gtggtacatt tacaatgaca
    29521 tttgaccact acgaagatgt accaaaatca gtacaagaag aaatcattaa gaaaaacggc
    29581 ggaaaagctg aataatttct taaaaccgga tagtcctgca aagagattat ccaaagagac
    29641 tatattttta atatagtttc gtgtaaaata attatcgatg atatgggcat gtggtgtacc
    29701 ttgtgtacat catataccta tcagacaaat aaaaaatata ttgtattttt aggaggacat
    29761 tttaaaatgg caaaagaaaa atttgaccgt tctaaaccac acgttaacat tggtactatc
    29821 ggacacgttg accatggtaa aactacatta acagctgcaa tcacaactgt actatctaag
    29881 aaaaacggcg gccaagctat ggcttacgat caaatcgatg gtgctccaga agaacgcgaa
    29941 cgcggaatca caatctcaac agctcacgtt gagtatgaaa cagatacacg ccactacgct
    30001 cacgttgact gcccaggaca cgcggattac gttaaaaaca tgatcactgg tgctgctcaa
    30061 atggacggag ctatcttggt agtttctgct gctgacggcc caatgcctca aactcgtgaa
    30121 cacatcctat tgtctcgtca agttggtgtt ccttacatcg ttgtattctt gaacaaagta
    30181 gacatggttg atgacgaaga attactagaa ttagttgaaa tggaagttcg tgacctatta
    30241 acagaatacg aattccctgg tgacgatgtt cctgtagttg ctggatcagc tttgaaagct
    30301 ctagaaggcg acgcttcata cgaagaaaaa attcttgaat taatggctgc agttgacgaa
    30361 tacatcccaa ctccagaacg tgacaacgac aaaccattca tgatgccagt tgaagacgtg
    30421 ttctcaatta ctggacgtgg tactgttgct acaggtcgtg ttgaacgtgg acaagttcgc
    30481 gttggtgacg aagttgaagt tgttggtatt gctgaagaaa cttcaaaaac aacagttact
    30541 ggtgttgaaa tgttccgtaa attgttagac tacgctgaag ctggagacaa cattggtgct
    30601 ttactacgtg gtgttgcacg tgaagacatc caacgtggac aagttttagc taaaccaggt
    30661 acaatcacac ctcatacaaa attctctgca gaagtatacg tgttgacaaa agaagaaggt
    30721 ggacgtcata ctccattctt cactaactac cgtccacaat tctacttccg tacaactgac
    30781 gtaacaggtg ttgttgaatt accagaagga actgaaatgg ttatgcctgg cgacaacgta
    30841 acaatggaag ttgaattaat ccacccaatc gctatcgaaa acggtactaa attctcaatc
    30901 cgtgaaggcg gacgtacagt tggtgccggt gtcgttacag aaatcaaagc ttaattcact
    30961 taagttttaa agtaaagcaa catttattcg gacgtaatcg ctcggaacga aggtcttagg
    31021 atctttgttc cgagcttttt tatttattgc aggatcagga aaaaagacag gagtattata
    31081 aatagaagaa acacgaaata aaagagtcag tattttctaa atgaaacttt atgaaaacaa
    31141 aatgtttttt acaagaaaaa gccttgcttt gtgagtttat atatattata atgacaaagg
    31201 tgcgtttact ttagtgaatt gcacagacgt aatcagaatg atttcgtcgg ctaagaaacg
    31261 agaggttgcg acacgcccgg acgctttgcc acgaacgagc gtgacggaaa ttttcgtgga
    31321 gctatgtcta cttttcaaaa taggcgaagg agggaacaag atggcaaaac aaaaaattcg
    31381 tatccgttta aaagcgtatg aacaccgtat tttagatcaa tcagcggata aaatcgtgga
    31441 aactgcaaaa agaactggag ctagcgtgtc tggtccaatt ccattaccaa ctgaacgcag
    31501 tctttacaca atcatccgtg cgactcataa atacaaagat tcacgcgaac aattcgaaat
    31561 gcgtacacac aaacgtctta tcgacattgt gaacccaaca ccaaaaactg ttgatgcttt
    31621 gatgaagcta gacttaccat ctggtgtaaa catcgaaatc aaattataaa actaaaacaa
    31681 aaataatgga ggtgtactca tgaccaaagg aatcttaggg aaaaaagtgg gaatgacaca
    31741 aatcttcact gaatctggtg aattaattcc agtaactgtt gtagaagcta ctccaaacgt
    31801 agtactacaa gtgaaaacaa tggaaacgga tggctacgaa gctattcaag ttggttacca
    31861 agatatgcgt gaagttttat caaacaaacc tgcgaaaggt catgttgcaa aagcaaacac
    31921 ggctcctaag cgcttcattc gtgaattcaa aaatgttgag ctaggagaat atgaagtagg
    31981 aaaagaaatt acagtagatg ttttccaagc aggagacatt attgatgtaa caggtactac
    32041 gaaaggtaaa ggattccaag gggttatcaa acgccacgga caaagccgcg gaccaatggc
    32101 tcacggttct cgttaccatc gtcgtcctgg gtcaatgggt ccagttgcac ctaaccgtgt
    32161 atttaaaaac aaacgtcttg ctggccgtat gggtggcaac cgcgtaacta tccaaaactt
    32221 ggaagttgta cgtgtcgatg cagaaagaaa tgttatcttg atcaaaggta atgttcctgg
    32281 agctaaaaaa tctttaatta ctatcaaatc agctgtgaaa gctaaataat tgtaggaaga
    32341 aaggaggaac taaggaatgc cgaatgtagc attattcaaa caagatggaa gccaaaacgg
    32401 tgaaatcaca cttaacgaag aaatcttcgg aattgaacct aatgaaagtg ttgtttacga
    32461 tgcaatcgta atgcaacgtg cgtcattaag acaaggaaca cacgcggtta aaaaccgtag
    32521 cgctgttcgc ggcggcggac gtaaaccatg gcgtcaaaaa ggaacaggtc gtgcacgtca
    32581 aggatctatc cgctcaccac aatggcgtgg aggtggcgta gtctttggac caacaccacg
    32641 ttcttacagc tacaaacttc ctaaaaaagt tcgtcgttta gcaatgaaat ctgtattgtc
    32701 agaaaaagtt gctgaaaaca acttggtagc aatcgaaggc ttgaacttcg acgcaccaaa
    32761 aacaaaagaa ttcaaacaag ttcttgctaa cttatctatc gactctaaag tattggttgt
    32821 tctagaaaaa ggtaacgact ttgctgcttt atcagcacgt aacttaccaa acgtttctgt
    32881 agtaacttct gataatgtga gtgtgctaga cgttgtatca aatacaaaag tgttagcaac
    32941 acaaactgct cttactcaaa ttgaggaggt gcttgcataa tgaacttact agacgtaatc
    33001 aaacgcccag tgatcactga aaaatcaatg cttgccatgg atgacaagaa atacactttt
    33061 gaagtggaca ctcgcgcaaa caaaacttta gtaaaacaag cggtagaagc tgcttttgac
    33121 gttaaagtca aaaacgtgaa catcgtgaac gtacgtgcga aattcaaacg tatgggcaaa
    33181 tatgcaggat atacaaaaaa acgtcgcaaa gcgatcgtta cattgactga agattcaaaa
    33241 gaaatccaat tattcgaagc tgctgaataa gcactaatag ataactcatt aaattaggag
    33301 ggaaaaacgt ggcgattaaa aagtacaaac ctacctcaaa tggtcgtcgt aacatgacag
    33361 gttctgattt tgctgaaatc acaacatcaa cacctgaaaa atcattgtta caaccattga
    33421 aaaaccatgc tggacgtaac aacaacggcc gcatcactgt acgtcatcaa ggtggcggac
    33481 acaaacgcca atatcgcgtg atcgacttca aacgtaacaa agataacgtt gttgcaactg
    33541 ttcaaacgat cgaatatgat ccaaaccgtt cagctaacat cgcgttagta cactatgaag
    33601 atggagtgaa agcttacatc ttagcaccta aaggactaga agtgggtatg aaacttgttt
    33661 caggtccaga agcagatatc aaagtcggaa atgctcttcc attagaaaac attccagttg
    33721 gtactgttat ccacaacatt gaaatgaaac ctggtaaagg tggacaattg atccgttctg
    33781 ctggtacaag tgctcaagta cttggtaaag aaggcaaata cgtattgatc cgcttgaact
    33841 ctggtgaagt tcgtatgatc ttagctgcat gccgtgctac aatcggttct gtaggtaacg
    33901 aacaacacga attaatcaac atcggtaaag ctggacgctc tcgttggatg cgtaaacgcc
    33961 caactgtacg tggtagcgta atgaacccta atgatcaccc acacggtggt ggtgaaggta
    34021 aagctcctat cggacgtaaa gcaccagttt ctccttgggg tcaaccagct cttggttaca
    34081 aaactcgtaa caaaaaagct aaatcagaca aacttattgt tcgtcgtcgc aaaaataaat
    34141 aattactttg accatgtgtc gcacattttg agaggaggtt caccatgggt cgtagtttaa
    34201 agaaaggacc ttttgtcgat gagcatttaa tgaaaaaagt tgaagctcaa gcaggggttg
    34261 aaaagaaaaa agttatcaaa acttggtcac gccgttcaac agttttccca agttttgttg
    34321 gatataccat cgcagtttat gatggacgta aacatgtacc agtttacatc caagaagaca
    34381 tggtaggaca caaattaggt gaatttgcac ctacaagaac ttatcgtgga catgctgctg
    34441 acgataagaa aacgaaacgc taatttgagg ggaggataaa tccaaatggc agaacaaatc
    34501 acatcagcta aagcaactgc aaaaacagtt cgcacttcac ctcgaaaagc ccgtttagta
    34561 attgacctta tcagaggtaa aagtgtcgca gatgcaattt caatcttgaa attcacacca
    34621 aacaaatctg ctggaatcat tgaaaaagta ttgatgtcag cagttgcgaa tgcagaaaat
    34681 aactttgact tagatgttga aaacttggta gtaagcgaag cttttgttaa cgaaggacct
    34741 acaatgaaac gtttccgtcc tcgtgcaaaa ggatcagctt ctccaatcaa caaacgtaca
    34801 agtcatatca cggtagtcgt atcagaaaaa taaggaggga taatcagtgg gtcaaaaagt
    34861 acatccaatt ggaatgcgtg taggcatcat ccgcgactgg gatgctaaat ggtatgctga
    34921 aaaagagtat gctgagttct tacacgaaga tttaagaatc cgtaaattta tttcaactaa
    34981 acttgctgat gctgctgtat caacagttga aatcgaacgc gcaacaggtc gcgtaaacat
    35041 ttcaattcac acagctaaac caggtatggt tattggtaaa ggcggttctg aagtcgaaag
    35101 cctaagaaaa gaattaaaca aattaactgg taaaagaatc catatcaata tcgtggaaat
    35161 caaaaaacca gatctagatg caaaattagt aggtgaagga atcgcacgtc aattagaaaa
    35221 ccgtgttgct ttccgtcgcg ctcaaaaaca agccatccaa cgctcaatgc gtgctggcgc
    35281 tcaagggatc aaaactcaag tatctggtcg tctaaacgga gcagatatcg ctcgttcaga
    35341 aggatactca gaaggaactg ttcctcttca tactttacgt gcggacattg attacgcatg
    35401 ggaagaagca gatacaacat acggaaaact aggagttaaa gtgtggatct atcgtggaga
    35461 aattcttcca actaaaaaaa acactgagaa aggagggaaa taatcatgtt agtacctaaa
    35521 cgtgtaaaac accgtcgtga attccgcgga aaaatgcgcg gggaagctaa aggcggaaaa
    35581 gaagtagcat tcggtgaata cggtttgcaa gctgttgatt cacattggat cacaaaccgc
    35641 caaatcgaag ctgctcgtat cgcaatgact cgttacatga aacgtggtgg gaaagtatgg
    35701 attaaaattt tccctcacaa atcttatact gccaaagcaa ttggggtacg tatgggttct
    35761 ggtaaagggg cacctgaagg atgggttgca ccagtaaaac gtggtaaaat catgtttgaa
    35821 atcgcaggcg ttcctgaaga agtagctcgt gaagcgttac gtctagcttc tcacaaatta
    35881 ccaatgaaaa ctaagatcgt aaaacgtgag gaaatgggtg gtgaatcgaa tgaaggttaa
    35941 agaaatcaga gaattaacca ctgccgaaat gcttgatcaa gaaaaacaat taaaagaaga
    36001 attgtttaat cttagattcc aattagcaac aggtcaatta gaaaacactg cacgtattaa
    36061 agaagtacgt aaatcgattg cacgcatcaa aactgtgttg cgtgaacaag ctaagtaatt
    36121 atggaaggag gccattaagc gtatgactga aggaagaaat caacgcaaag tttatcaagg
    36181 tcgcgtggtt tcagacaaaa tggataaaac aattaccgtt gctgtcgaaa caaagaaaaa
    36241 ccaccctatc tatggcaaac gcatgaacta ttctaagaaa tacaaagtgc atgatgaaaa
    36301 caacacagca aaagttggcg acatcgtgag aattatggaa actcgtccat tatcagctac
    36361 aaaacgtttc cgtttgttgg aagtagtcga agaagcagtt attatctaat aacacgagat
    36421 tttcctatat agattgaatg caaaatctga aaggaggata cacaacgtga tccaagcaga
    36481 aagtcgttta aaagtcgctg ataactcagg cgcacgtgaa attttgacga tcaaagtcct
    36541 tggtggatct ggtcgtaaaa ctgctaacat cggtgatgtg atcgttgcta cggttaaaca
    36601 agcaacgcca ggtggggttg tcaaaaaagg tgacgtcgtt aaagccgtta tcgttcgaac
    36661 aaaatcagga gctcgtcgta cagacggttc atatattaaa tttgatgaaa atgctgcagt
    36721 gattattcgt gatgataaaa gccctcgtgg aacacgtatc tttgggccag ttgcacgtga
    36781 actacgtgaa aacaacttca tgaagatcgt ttcactagca ccagaagttt tataatcttg
    36841 agacacttat caaaggaggt gcgaaacagt tatgtttgtt aaaaaaggcg ataaagttaa
    36901 agttatcact ggaaaagaca aaaacaaaga aggcgttgta ctagcagcgt tcccgaaaaa
    36961 agataaagtt attgtcgaag gtgtaaatgt cgttaaaaaa caccaaaaac cttctcaagc
    37021 tgctcctcaa ggtggaattg ttgagatgga agcgccaatt catgtttcta acgtgatggt
    37081 ggttgactct actggtgtag ctggccgagt tggctacaaa gaagtagacg gcaaaaaagt
    37141 ccgtgtttct aaaaaaaccg gtgaagtttt agataaataa aagtttaagg aaggagggtc
    37201 ttactgaatg aaccgcctaa aagaaaaata tcttaaagaa gtaactccat cattgatgga
    37261 aaaatttaac tatagctctg ttatgcaaac acctaaagtt gaaaaaatcg tcatcaacat
    37321 gggtgttggt gatgcggtat caaacgctaa aaacttagat aaagcagttg aagaattaac
    37381 tttgatcact ggtcaaaaac caatgattac aaaagctaaa aaatcaatcg ctggattccg
    37441 tttacgtgaa ggaatgccaa ttggtgcaaa agttacattg cgcggagaaa gaatgtacga
    37501 attcttagac aaattggttt ctgtttcact accacgtgta cgtgacttcc atggtgtaag
    37561 taaaaaagcc tttgacggtc gtggaaacta tactttaggt attaaagaac aattgatctt
    37621 cccagaagta gattatgatt tagtagataa agtacgtggt atggacatcg ttattgtaac
    37681 aacagcaaac tctgatgaag aatctcgtga gttgttgaca caattaggca tgccattcca
    37741 aaaataatta aaggaggcga actacgtggc taaaaaatca atgattgcta aaaacaaacg
    37801 tcctgcaaaa ttttcaacac aagcttatac tcgttgcgaa cgttgcggac gtccacattc
    37861 agtttatcgt aagttccatc tttgccgtat ttgcttccgc gaacttgcct ataaaggtca
    37921 aattcccggc gtgaagaaag ctagctggta aaaagtaaat tcgcataaga aggaggtaaa
    37981 agatcaatgg tcatgacaga tccaattgca gattttctaa ctcgtatccg taacgcaaac
    38041 atggttaaac atgagtcttt agaagtacct gcatcaaaaa ttaaacgtga catcgctgaa
    38101 atcttgaaac gtgaaggttt cgttcgtgat gttgaataca tcgaagatga caaacaaggc
    38161 gtgatccgtg ttttcctaaa atttggtaaa aacggcgaac gtgttatcac aaacttaaaa
    38221 cgtatctcta aaccaggttt gcgtgcttat gtcaaagctg acgaagtacc aaaagtattg
    38281 aacggtttag gtatcgcaat catttcgact tctgaaggtg ttgtcactga taaagaagct
    38341 cgtgaaaaaa atatcggcgg cgaagttatc gcttacgtat ggtaatcaag tagaatctac
    38401 aaggaggtgt ctttaagtga gccgtatcgg aaataaagtc gttgttatcc ctgaaggagt
    38461 aacagttact caagacggaa acaacgttac agttaaagga ccaaaaggtg aattgactcg
    38521 tactttttca gcagatatca aaatgaatat cgaaggaaac gaagtaactt tcactcgtcc
    38581 aaacgacagt aaagaaatga aaacaattca tggaacaact cgtgcgaact tcaacaacat
    38641 ggttgtaggt gtcagcgaag gtttccaaaa agcgttagaa ctaatcgggg ttgggtaccg
    38701 tgcacaatta caaggatcaa aacttgtatt gaacgttggt tactcacatc cagtagaaat
    38761 cacaccacca gctggagtaa ctgttgaagt accagctaac acacaagtaa ttgttaaagg
    38821 tgcgaacaaa gaagaagttg gcgaattagc tgcaaacatt cgtggaactc gtcctccaga
    38881 accttataaa ggtaaaggta ttcgttatgt tggcgaatat gtacgccgta aagaaggtaa
    38941 aactggtaaa taatcgttta gaaaaattgc atagaagcca gcagcaggaa aaaggcattt
    39001 ggctgaagtt taccttcaaa aaaatacatt tttacttgtg ccgcagcttc tatttttcga
    39061 aacgtattta ctgcttgaaa tagtagtaca ataaaaataa agaggtgaca attgtgatta
    39121 caaaaccaga taagaacaaa acacgtcaaa agagacatcg tcgtgtacgt aacaatatct
    39181 ctggtactgc tgagcgccca cgcttgaacg tttttcgttc taacaaaaac atctacgctc
    39241 aaattattga tgacgtagcg ggtgtaacgc tagcaagtgc ctctaccttg gataaagaaa
    39301 tttcaggtgg aacaaaaaca gaagcagcac aagctgtcgg taaactagtt gctgaacgtg
    39361 cagctgaaaa aggcattaaa gtagtagtct ttgaccgtgg tggatacctt taccatggcc
    39421 gtgtgcaagc tttagctgaa gctgctcgcg aaaatggact agaattttag aagaaggagg
    39481 aataccattc atggtttata tcgatccaaa acatttggaa ttagaagacc gcgttgttgc
    39541 aattaaccgt gtaacaaaag ttgttaaagg tggacgtcgt ctacgttttg ctgctttagt
    39601 tgttgtcggt gacaaaaacg gacacgtagg gtttggtaca ggtaaagctc aagaagttcc
    39661 tgaagcaatc cgtaaagcaa ttgaagatgc gaagaaaaac ttagttgaag tacctatggt
    39721 tggttcaaca atcccacacg aagtaatcgg tgcttttagt ggtggtagaa tcctaatgaa
    39781 acctgctgta gaaggttctg gggttgctgc tggtggacca gttcgtgccg tattagaatt
    39841 agctggggta gcagatatca cttctaaatc tttaggctca aatacaccaa tcaacgttgt
    39901 tcgcgcaaca gttgaaggtt tgaaacaatt gaaacgtgca gaagaagttg cagaacttcg
    39961 cggcaaatca gttgaagaat taatcggata aggaggacaa caataatggc tgaattaaaa
    40021 gttactttaa aacgcagtat tatcggacgt cctcaaaacc aacgcgatac agttaaagcg
    40081 ttaggtctta caaagattaa cagttcagtg gttaagcctt ctaatgaagc aattaaaggc
    40141 atgatcaata ctgtttctca cctagtggac gttgaagaaa tttaatttag aaaaatcata
    40201 actagaacat taaggaggtg ccaaacctat gaaacttcat gaattaaaac ctgctgaagg
    40261 ttcacgccaa gtacgtaacc gtgttggtcg tggtacttca tctggtaacg gaaaaactgc
    40321 cggacgcgga caaaaaggac aaaaagctcg ttcaggcggc ggtgtgcgtt taggatttga
    40381 agggggacaa acaccattgt tccgtcgttt accaaaacga ggattcacaa acgtgaaccg
    40441 taaagactat gcagtcatta atcttgatgt attgaaccgc tttgaagacg gagctgaagt
    40501 aactcctgtt gctttagttg aagctggaat cgtgaaaaac gaaaaagctg gaatcaaagt
    40561 attagctaat ggggaactaa acaagaaatt aactgtgaaa gcagcaaaat tctctaaagc
    40621 agctcaagaa gcaattgaag cggctggtgg atcaatcgag gtgatctaat gtttagacta
    40681 ttaaaagatg cttttaaagt aaaagacatt agatcgaaaa tcctatttac tgtatttgtg
    40741 ttgtttgtat ttcgcctagg tgcacatatt actgtaccag gcgtaaatgc tgcttcattg
    40801 caaaaccttt cttctttgcc atttttcaac atgttgaact tggtaagtgg tagtgcgatg
    40861 caaaactttt ccattttctc aatgggggtt tcaccataca ttactgcttc tatcgtcatt
    40921 cagcttcttc aaatggacat cgtgcctcga tttgttgaat ggtcgaaaca aggggaagtt
    40981 ggtcgtaaaa aactgaatca agcgactcga tatctgacat tagtattggc atttatccaa
    41041 tctgtcggta tcactgctgg attcaatcaa tacgctcaat taggtttcgt gaataatcct
    41101 aacgtagcaa cttacgtaac gatcggtttg attctaacag ccggtacgat gtttgttact
    41161 tggctaggag aacagatcac agaaaaaggt atcggaaatg gcgtatcaat gatcattttt
    41221 gcaggtatca tttctagatt gcctgaaagt atcagagaaa tttatgaaga ttactttata
    41281 aatattgatc cttcagacat atggaaatct gctatttttg tggccatttt actagtagcg
    41341 gtcttgttga tcgtgacatt cgtaacattt ttccaacaag ctgaacgcaa gatcccgatc
    41401 caatatacga aacgcgtagc tggcgcgcct acaagcagct atctgccgtt gaaagtaaat
    41461 gcagctggag ttattccagt tatctttgct agttcgttta ttgctacgcc aaatgctgtt
    41521 ttacaagcat tgagcggtag attcggttct gaaaactggt acaaagtgat gatggagatc
    41581 tttaactata atacagcacc tggtgctacg atttatacga ttctgatcgt tgccttcact
    41641 ttcttctacg cctttgttca agtaaaccct gagaaattag cggaaaactt gcaaaagcaa
    41701 ggaagttata taccaagcgt acgccctggt aaggggactg aagagtatgt ctcaagatta
    41761 ctgatgcgtt taagtacagt tggtgcagtc ttccttggtt tagtggcatt gcttccaatc
    41821 attgctcaaa tggtttggga tcttccacaa tcaatcggat taggcggtac aagcttactg
    41881 atcgttatcg gtgtagcttt agaaactgcg aaacagttag aaggcttaat gctgaaacgt
    41941 aaatatgttg gattcattga gtaagaaagc gtgttgaggt ttcccctcaa cacacgttct
    42001 cacacattta ttaggaggga aaacaatgaa cctcatttta atggggctgc caggcgcagg
    42061 taaaggaaca caagctgaaa aaatcattga tgcttatggt attccgcaca tctcaacagg
    42121 agatatgttc cgcgcagcga tgcaaaacga aacagctctt ggcctggaag caaagtctta
    42181 tatggataaa ggtgcattag ttcctgatga agtaacaaat ggaatcgtga aagagcgact
    42241 agctgaacca gatacagaga aaggcttctt attagatggc tttccacgta cgctagatca
    42301 agcaaaagct ctggacgcaa tgctaaaaga tttgaataaa aaaattgatg ctgtcatcga
    42361 tatccatgtt ggtgaagaaa tcttggtcga acgtctagcc ggccgattta tctgcagcaa
    42421 ttgtggagca acttaccata aaattttcaa ccctacaaaa gttgaagaca catgtgatcg
    42481 ttgcggtggg catgaattct atcaaagaga agatgataaa cctgagacgg ttaaaaatcg
    42541 tctggctatc aacatcaaaa atagtgaacc aatcttggct tattataaag aacaaggttt
    42601 gctaaacact attgatggtg ctcgtgaaat cgatgctgta tttgcagatg tacaaaaaat
    42661 cattgaaaaa tagcacgatt gcccgtagcc gacttgcatt atgcagttac tcttgtcaag
    42721 ttcaataaat tatgatagaa tatgtcagtc cgacttctag agaatcctct agatgatcca
    42781 ttttgagaat tttgaggtgg gaactgcagt ttccgccatt ttatcgtgtg aaaatgcgaa
    42841 acaagtacaa ggaggtactg tgcgtggcaa aagaagatat gattgaagtc gaaggtacag
    42901 tcgtcgaaac tttgccgaat gcaatgttta aagttgaact agaaaacgga caccaagttc
    42961 ttgctactgt ttcaggtaaa attcgtatgc attacatccg tatcttacct ggtgacaaag
    43021 taacggttga attgtctcca tacgatctta accgtggccg tattacttat cgctttaaat
    43081 aattgtactc cgtaaaacta caggaggtat tatcatgaaa gtaagaccat cagtaaaacc
    43141 aatgtgcgaa cattgtaaag taattcgtcg taaaggacgc gttatggtga tttgcccagc
    43201 aaatccaaaa cataaacaac gtcaaggata attggaggtg ctataaatat ggctcgtatt
    43261 gcaggagtag atattcctcg tgataaacgt gtagtagttt cacttactta tatctatggt
    43321 attggtaaca ctactgctaa aaagatctta gctgatgctg gtgtatctga agatgttcgc
    43381 gttcgtgaat taacgaatga acaaacagat gctatccgtg cagaagtaga taaattaaaa
    43441 gttgaagggg atcttcgtcg tgaagtgaac ttgaacatca aacgtttgat ggaaatcggt
    43501 tcttaccgtg gaatccgtca tcgccgtgga ttgcctgttc gtggacaaaa cacgaaaaac
    43561 aatgcacgta ctcgtaaagg cccagctcgc gctatcgctg gcaagaaaaa ataattctta
    43621 agtgaaggag gttaaaactt catggcagca aaaaaagtga atcgtaaacg ccgtgtgaag
    43681 aagaatatag aagcaggtat tgcacatatc cattctacat tcaataacac tatcatcatg
    43741 atcactgata ctcacggaaa tgcattagcg tggtcatcag ctggttcatt aggtttcaaa
    43801 ggaagcaaaa aatcaactcc ttttgccgct caaatggcag cagaaactgc tacaaaagca
    43861 gcaatggaac acggattaaa gactgttgaa gtaacagtta aaggacctgg ttctggacgt
    43921 gaagcagcaa ttcgttcatt acaagctaca ggtttagaag tgactgcaat tcgtgacgtg
    43981 actccagttc ctcataatgg tgcccgccct ccaaaacgcc gtcgtgttta atgagtgccg
    44041 ctcattttga gttttatcag taacgtcatt gaacaagaca cttttcgttt tgaaaggggt
    44101 aaagataaga atgattgagt tcgaaaaacc aagaatcgca aaaattgatg aagaaaaaga
    44161 ttatggcaag ttcatcgttg aacctcttga aagagggtat gggactactt tagggaattc
    44221 cctacgtcgt attttattat cttctttgcc aggtgctgcc atcactaata ttcaaatcga
    44281 tggtgtactg catgaattct ctaccatcaa aggtgttaga gaagacgtca ctcaaatcat
    44341 cttgaacatt aaaggcttag cgctgaaaat gtatggccaa gaagaaaaaa cccttgaaat
    44401 cgatattacc ggacctgcta cagtaactgc tggcgatatt atcgttgata gtgatgtaga
    44461 aattcttaac aaagatatgt acatctgtag tgtctctgaa ggagctacgt tccatgctcg
    44521 cttaacagtg aaacctggac gtggttatgt tcaagcagat gaaaataaaa aagaagatat
    44581 gccaattggc gttcttccag tcgattcaat ctacacacct gttcgtcgtg tgaactatca
    44641 agttgagaac acacgtgttg gacaccgtga agattttgat aaattgacga tggagatttg
    44701 gacagatggt tcaattgaac cgttggaagc aatgagtttg tctgcgaaaa tcatgactga
    44761 acatttggat atttttgtga acttaactga tgaagcaaaa aatgctgaaa tcatggtcga
    44821 aaaagaagaa acacaaaaag aaaaaatgtt ggaaatgacg atcgaagagt tagacttgtc
    44881 tgttcgctct tacaattgtt tgaaacgtgc aggaatcaat actgtacaag aacttacaaa
    44941 taaatctgag cctgaaatga tcaaagtccg taacttagga cgtaaatcat tagaagaagt
    45001 taaagcaaaa ttgcacgact taggtttagg tttacgtaaa gaagattaaa accttttacg
    45061 aaggagggaa accaacgtga gttatcgtaa actaggacgc acatctagcc aacgtaaagc
    45121 gatgttgcgt gatttaacaa ctgatttatt aatcaacgaa cgtattgtga ctactgaagc
    45181 tcgtgcgaaa gaagttcgct caactgcaga aaaaatgatc actttaggta aacgcggaga
    45241 tttgcatgca cgtcgtcaag ctgcggcttt cgtacgtaat gaagttgcca gcgtgcgtga
    45301 agaaaatgag gacatcgtca ttgaatcagc tttacaaaaa ctgttcaacg atattgcacc
    45361 tcgttacgca gaacgtcaag gcggatacac acgcattttg aagacagaac caagacgcgg
    45421 tgatggtgca ccaatggttg tcttagaact tgtctaaaat cacactaata acatcacttt
    45481 atagatgatt cttttagagt gttatgatgt tggaagatat ttttccgagt ctagctcagc
    45541 gctgctcctc aagcgaatat cgcttgggga gtaagcactc atcataaagt gtttttttat
    45601 accttcaatt acctgtcatg acagatatgt ttgaaggcat aaaaaaacat tttaagcgag
    45661 aaataattga aaattttgtc tttttttgat agtgttagta ggaactttag gatgaagaag
    45721 gagtaatcat ggagccaatt attgaattag aaaagataaa ttataaatac cagccagatg
    45781 atcttcgccc cgcattgaaa gatgtctcct ttacaattga taaaggtgag tggatcgcta
    45841 ttattggaca caatggttca ggaaaatcga cacttgccaa aacgatcaat ggattgcttc
    45901 tgccagaatc agggatcgtg aaagtgggaa atcaaatatt ggatgaagaa aatatttgga
    45961 cgatccgaca gatggttgga atgatttttc aaaatccaga caatcagttt gttggttcga
    46021 cagtagaaga tgacgttgct tttggtcttg aaaatcaagg gattcccaga gaagaaatgc
    46081 tagtgcgtgt gaaagatgca cttgagaaag ttagaatggc tgagtttgct tcacgcgagc
    46141 ctgctcgttt atcaggaggg caaaaacaac gtgttgccat tgccggcgtg gttgccttac
    46201 gacctgatat cattatttta gatgaagcaa ccagtatgct agatccagaa ggtcgtgaag
    46261 aagtgatctc tacgatcaag aagatcaaag aagaaagtca attgaccgtt atatctatca
    46321 ctcatgatat tgatgaagca gctaatgcca atcggatact agtgatgaga caaggcgaac
    46381 ttgtccgtga aggtacacca aaagaaattt tttctgctgg tcctgaactg atcgacttag
    46441 gtctagatct accatttcct gaaaaactga agagcgcact gaaagaacgt ggggttgatg
    46501 tgcccagtga atatatgaca gaagaaagga tggtggattg gttatggaca tccgttttga
    46561 acaagtagac ttcacttatc agccgaatac accatttgaa caaagagcct tatttgacat
    46621 caatatgacg atcaaagaaa acagttatac tgcattagta gggcataccg gaagcgggaa
    46681 atcaacctta cttcagcatt tgaatgcgct tgttaagcca acaagcggta cagtccatat
    46741 tggagaacga gatattcagc cggatacgga taataagaat ttaaagccca tccgaaaaaa
    46801 agttggcatc gtctttcagt ttccagaagc acagctattc gaagaaacgg tagcaaaaga
    46861 cattgctttc ggtcctaaaa actttggtgt cagcgaagaa gaagcactag tcctagcaaa
    46921 agaaacatta gaacaagttg ggctggatga aagctatttg gaacgttcgc catttgaact
    46981 ttcaggaggg caaatgcgta gagtagcaat cgctggtgtg cttgccatga ggccggaagt
    47041 acttgtattg gatgaaccaa cggcaggact tgatccccag ggcagaaaag aaatgatgga
    47101 gatgttttgg cgtcttcata aggaacagca gatcactatt gtattagtta cgcatttaat
    47161 ggatgatgta gccaattttg cagattatgt ctatgtgttg gaaaagggac gaatcgttaa
    47221 tagcggggag ccgcaagagg tctttcaaaa cattgaatgg ctaaaagaaa aacaactagg
    47281 cgtacctact gccaccgagt ttgcagaaga attaatggct aaagggatga actttgctac
    47341 cttgcctttg acagctgaag aattagcaga cgctatcgtc cattatgctg gaggaaatgc
    47401 ccatgatgaa taaattgata tttggtcgtt atatgcctgg agattccttg atccatcgat
    47461 tggatccccg agctaaatta ttagccagtt tttattttat cggaatcatt tttttagcaa
    47521 ataattggga aacgtacctt ttgttggcat ttttcacatt actttctgtc ttcttgtcaa
    47581 aagtagattt gaaatttttt atccgaggca ttcgcccatt gatctggcta atcctcttca
    47641 ctgtagcttt gcaggtcttg tttacaagtg gcggagaaac ttattggagt tggggtgttt
    47701 tccatatcac tgaatttggt gtgatgaacg gcctctttat tttctgtcga ttcgtgttga
    47761 tcatttttat gtcgactcta ctcacgctta ctacaccgcc tttggaatta tcggatgcga
    47821 ttgagtactt gcttcgtcca ttgaaagccg tacggttccc agtccacgaa atttcattga
    47881 tgctgtcgat tgctcttcgt tttgtgccta cattgatgga cgaaacagaa aagatcatga
    47941 acgctcagcg cgctcgcggt gttgattttg gtgaaggaaa cctagttcag aaaatgaaag
    48001 cagtcattcc tttgctgatt cctttatttg tcagcagctt caaccgtgcg gaagatcttg
    48061 cgactgctat ggaagcaaga ggctacaaag gtggggaagg aagaacgaaa tatcgtgtat
    48121 tgcattggca taatagagac acgatggtta tcattgcttt tgtattatta actgtgctac
    48181 tgatcttttt aagaggataa acgttcagtg ggccgctatc tattcttttc ttgctgatct
    48241 gtttgcaaga aaggatgcaa gcagtgtatg acaagaaata ttagggtctg tggcagacta
    48301 aggtcacagg cctctttctt ttatctgaaa agaaagaacg aaaaaatttt atagaaaaat
    48361 cattattcca gataaccttt taggaacaaa ggagagaaaa acatggtacg ttacaaagca
    48421 atcattgcgt acgacggaac aaattttaat ggattccaaa aacagccaaa tgggcgtact
    48481 gttcaagaag aagtagaaaa aacattacaa aaaatggcaa atggaaaaga aatcacggtc
    48541 tttggttcgg gaaggacaga tgccggagta catgcgatcg ggcaagtaat ccattttgat
    48601 tatccagagg agcgtccgtt agaacgaatg cgtttcgcgc tagataccca gtcaccagag
    48661 gatattgctg taagacaagt agaaattgtt tcagaagact tccacgcaag atatttggtc
    48721 aaagaaaaga cgtatcaatt tcgagtggat atagggaaac cacgcagtcc ttttcgacgc
    48781 cattatgcaa gttattttcc ttacccactt gatttggaaa aaattcagcg ggcgcttcct
    48841 gatttattag ggactcacga tttcacctct ttttgtgcat caggaagctc gatagaggat
    48901 aaaatacgta cgatctacga ggctaagatg gaggtaaatg aaacaaaaga cgaattgttg
    48961 tttacattta ggggaaatgg cttcttgtat aagatgatac ggatcttagt aggaacgctt
    49021 ttgaaaattg gcaatggccg attgcctgaa gatagtatcc cagatattat tgcgaaaaaa
    49081 gatcgtaatg cagcaggacc tacagcacac cctgaaggac tttatttgta tgaagtgaaa
    49141 tatgaataag aggttatgat agaagtggtc agcttcaaga aataagaagg tatttacgaa
    49201 aattacttct caaaattttg caaaatttcg gcttattttc gaagaagcta cttctggaac
    49261 accgtttaag aaagaggtcg tgagcctgtc atttaactct gagtcacaaa gagcaatttc
    49321 aaaaaacagc ttctcatctt tgatgaatat cgtgacttgt ggacgaggcg ttgataagtg
    49381 aatcatcttt ttgttaccta ttttttctgt aaccaaaaaa cgatggaacg ttgttttgat
    49441 tgcccttggt gcggacagtc tgaacaggtt ccatcgtttc tttattttct acgtcttctt
    49501 ctgtgtgtgt ttcgtggttg ttcttctatt gctggatcaa tcgtgccata aaaggggcga
    49561 ttgcggtcaa ggtaccagac ataaagaata aacaatgcaa tacataggac aaaaaggatt
    49621 gcacctatcg taaatccttc aggcaagtaa ctgaaagtga cagtatgttt acctgctgga
    49681 acttttatca tcaagaatgc gtctttgaaa ttgctgacct tgactggttt cccatcaatt
    49741 ttagctttcc atcctttgtc ataaggaata gttgtcacca acatggaatc ttcagcggta
    49801 tttacagtcc cagaagcttt tctgccagtg gcttccattt cgacaccttt ttctttgatc
    49861 tgattcattg cggcttgata ggctacaaca tctagaccaa cgactttagg ctccatgaaa
    49921 cttatttctt tcgtcccata aaaacttgca gtaaatgtca ctgttgtctc ttgaggataa
    49981 tagccaagat catagtattg accagaaatg ttgatttgcg tttttcgcga tgtaccattg
    50041 acagtgatcg tcgcactaga actttctaat tgcgcaaaat tggttgggaa caaacttaag
    50101 taagcttgtg tatttgcagg cacatggact tcccaagtga gctcttttgg ctggttatct
    50161 gctgtttctg agtaagtgac gccagtcgtt gtctttgtga tcgtcgtatt caactgattc
    50221 ttcaaagtag gtgtatagaa tgtaaaatac tgttcattta gaccagacaa tgtattgatc
    50281 aagtttgtct gactggtcaa attatctgtc actggctgct caacatcatt aacggacaac
    50341 ggtgcagtaa atgctagtgg caatgcattg acattctgat acagtgtata gtcgccgctt
    50401 tgaccagctt ggcggaagcc atatttactg aacgtgcttt cgtttttaga aatgttatat
    50461 ttgattcctg taaacccatc catcagcaaa gtattattgt tataacggat attcaaattt
    50521 gtccctcttg aacggaaacc taatttatcc aggtaagagg aagaattacg atttcttata
    50581 gaagaaaaca aactgatacc actgtaacca tagttgatcg aatcgtttgc agatactgga
    50641 tccagatttt ccaagcggta aaacgagtcg gaatcttttt ttgtttcatt tacaagagct
    50701 ttcaagtcag gataaggatc agtatataaa ctgcgggaag cataattcca atcatcaaga
    50761 atgccatgga tcatactgtt cgtattgacc agtgcttcag cagacatgat caataataac
    50821 aatatactca gatgattgaa tgaaagtttt ttatgctgat aaaatcccac agcaaaagca
    50881 taaatcccta agaaaaccac tgtcaataca aaggaagtca tcgtcacata ggtgtagctt
    50941 ccttcaggtt tgatgcccca tgccaccgca aagagagcag ctacgaccaa aatgattcct
    51001 aacaacagcc caccatcgtc ttgttctatc ttttcccatc cttttcctgc aagcaggagc
    51061 actaagaaag agaacagata ggcataacgg aataaaaaca tattcggtgc atgcatacca
    51121 tgccagaaca aattcaatgg aacaagataa aaacttgcga taagtatggc gaaaagactg
    51181 ccgtacaaaa atttattttt caacggtact ttccgactgg caaaataaaa cagacagagg
    51241 actaatggta gcagacctgc gtaaacgaac ggaatcgatc cgtattttgt cgtgtcataa
    51301 acaccaatca tatttttcat gattagatcc agataagaag tcgcttctgt tttcaacgta
    51361 gtgatctgac tcagtgcctc gccatttgac cgaagatcca gcactgctgg caatacaatg
    51421 atcattgaag cgccgcctgc cagtaaagaa gtgataccat aaggaacaat cgttttttta
    51481 tattctgacc agtttgtcaa tagacgggcg ataaaatata agaaagaaaa tacgccaatc
    51541 ataaaaccca tataaaaact ggataaaaac aagagtaaat aactgataaa taaaagtttc
    51601 ggccgtttaa gatccataac acgatgaatc cccaaaatca ctaatggaag ataaacaaat
    51661 gcatccagcc acatgatgat ctccgaatgt gcagtgataa atgagatcaa tgcgtaactg
    51721 acgctaagtg caacatgccc ccagcgagga atcttgaacg tacgtgcaag aaaccaaaaa
    51781 gccaatccgg cacagccgac cttaagcaaa gtcagaacat acagtgcatc tggcatcatc
    51841 tggtttggaa aaaagaacac cagtggcgtg aagatcccgc ctagatagta agaaattaaa
    51901 gataaataat tcaatcctag agaagcattc catgtataaa aaatactttg ctttccaaga
    51961 agaacatttc ggaagctggc atgaaaatta gaaaactgtg aaaaggcatc acttgccaaa
    52021 atacttctgc tgcttccagg atagattccg atacttagat aaacaagtgc taaaatcaaa
    52081 aacggaatca ggaaactagc tgctgcgtag cgtccgtttt ctctaaagaa tgactttaaa
    52141 ttttttttca tatttatttt ccaccaaatt tctatgaagt tgtattcagt ttatcatatc
    52201 cttgtttttc tctgcttaaa aagagtctag ttttcacaaa aaaataaaat ttagaataaa
    52261 taacttcata atttgttcgt ataacaaaaa taaacgcaaa aaaagggtag gaatcaaaag
    52321 tcccaccctt aaaaattttt attaaataaa aaaccgttca ctatctgaac ggtccaagta
    52381 tcattcaatt aaacgcgttc aacttttcct gatttcaaag cacgagttga cacccaaact
    52441 tttttaggtt ttccgtcgac cataacgcga actttttgta agttaggttt aacagtacgt
    52501 tttgttgagt tcatagcgtg tgaacggttg tttccacttt ttgtcttgcg acctgtaaag
    52561 taacaaactt ttgccatggt aatttcctcc tttgccttga tttggagcaa tagaataatt
    52621 cagctcatac cagactaaca tatcatatag aatgggaatt tgcaagtaca ttttcataga
    52681 tacaattgac aattgagaag aataattgta aattaaacaa ggttatacct gaaattttgc
    52741 aaggtttttg agtactttgg gtaactgcct tttatttcaa gttgcgctat gataaaatat
    52801 gatagaagaa aaatgggagt tttttacccg ttaaggaggc tcattcatgg ctgtaaaaat
    52861 tcaaacgtca gctggaacca ttgaaattac caatgatgta attgcaacag ttgttggtgg
    52921 cgccgcgaca gatgtgttcg gtgttgtagg aatggccagc aaaaatcaaa ttaaagataa
    52981 tattactgag attttgcgta aagaaaatta ctcaagagga gtagttgtcc gccaagaaga
    53041 taatggtatt gctgtagatg tttatacgat tatcagctat ggtacgaaaa tctctgaagt
    53101 ttcccgcaac gtccaagaaa aagtgaaata caatctagag gcattattag gcgtaactgc
    53161 caattcagtt aatgtctttg tacaaggtgt tcgtgtactg ccagactagg caggagactg
    53221 gctggtaact cagcagtcga tctgaaggag gattatctag gtgagtgtaa cagaaataag
    53281 tgcaagccag ttccaggaaa tggtccaggc aggtgcgaat cgtctgcaag taaatgcaga
    53341 atacgtcaat tcattaaatg tattccctgt gccagatggc gatacgggaa caaatatgaa
    53401 tctatctatg actagtggag ctaaggcagt agtagattca acctcagaaa aagtaggaga
    53461 attagcagca atgctttcaa aaggattgct gatgggtgcg cgcggaaatt ccggcgttat
    53521 tttgtctcaa ttgtttcgtg gattttctaa gcaaattcca gatgttgtct cattaaatgc
    53581 ttctgatctt gctgcagcct tcactcatgg agttgaaact gcgtataaag cagtaatgaa
    53641 accagtagaa ggaacgattt tgacagtagc tagagaagca gcgaaagctg gagaaaagaa
    53701 agcaaaaagt actgatgatg tcgttgaagt aatgacagct gttgtaaaag gagcaaagcg
    53761 tgctttagct aaaactccag acttgcttcc agtcctaaaa gaagtcggtg tcgtagacag
    53821 tggagggcaa ggactgctgt ttgtttatga gggcttttta agtgcattga atggagaatt
    53881 ccaagcggat gaaacatatg aaccttctcc agcagaaatg gatgatatgg tcaatgcaga
    53941 acatcatcgc agtgtccaag gtcaaatggc gacagaagat atccattttg gttactgtac
    54001 agaaatcatg gtaaaaatcg gagaaggacc tacagtcgat agtacattcg attacgaaga
    54061 attccgtaac tatttagatg gtataggaga ttctttactt gtggtcaatg atgacgagat
    54121 catcaaagtc cacgtgcata cagaaaaccc aggagaagtc ttgaattatg gacaaaaatt
    54181 cggttcatta gtcaaaatca aagtcgacaa catgcgcttg cagcatgaga cgattttgga
    54241 acacgatgaa gaagtgaacg agtttgcgaa aaaagaaata ccacgtatcc cgtatggtat
    54301 cgtagcgatc gctgctggta aaggcgtcca agagctgttt gaaagtttag gcgctaatta
    54361 tgttatcagt ggtggacaaa caatgaatcc aagtacggaa gacattgtta aagcgattca
    54421 agaagtaaac gcagataaag tgattgtctt gccaaacaat aagaacatct tcatggcagc
    54481 tgatcaagca gcagaagtag cagatatccc agtagcagtt gttccttcaa gaacgatttc
    54541 ccaaggattg acagctatgc tttcattcaa tgatcaagct tctttagaag aaaacaaaga
    54601 aacgatgaca gaaatgttgt ctagcgttgt gagcggtcaa gtcactcacg cgatcagaga
    54661 tacctcaatt gacggtgtag agatcaaaga aggagacttt ttgggcatga tcgatggaaa
    54721 aatcctgatc tctaacccag atatcacagc aacagcgtta agtacactag aacaaatgat
    54781 caatgaggac acagagatcg taacgatctt ggcaggtgat gaaggaacag ttgaacaagc
    54841 aaatgaattt gctgagaaac taacagaaaa acatcctgat ttggaaatcg agatccacca
    54901 aggagatcag cctgtctatc cttatctatt ttctgctgaa taatttctgt gtcagaatat
    54961 ctttctaaca cagtattcat tcataaaagg ccttcggtta gtgatcaggt atgatcgctg
    55021 accaagtcct ttttttgtta aaggagggag gaaaaattta tgcgttcttt agatgatcca
    55081 atcactatgc tggcaggagt cggagaaaaa agagcagcta gtcttgccag cttgggcatc
    55141 caaacgattg aagacttact gacttattat ccttttcgct acgaagatat tcaggaaaga
    55201 aatctaaacg agatccagga tcaagaaaag gttactttga aaggaatcgt agtctcgcct
    55261 cctgtgatga atcgatttgg ttataaaaaa agccgattgc aatttcgtat gatgcaagaa
    55321 catgatgtgt tcaacgtttc tttttttaat caaccttatc taaaagaaaa agtcatttta
    55381 tcggaggaaa tcgccgtata cggtaaatgg gatgcaaagc gtaaagcctt gaacgggatg
    55441 aagattttag gttctcaaac aatggatgac ttttcgccga tatatcacgt caacaaatca
    55501 attcgtcaaa cgacattagt tgatctaatc aaaaaagggt ttcaagaata cggcacattg
    55561 attgaagaaa atctgccaaa caaccttgtg gagaaatatc gactgcttga ccgagcttct
    55621 gctgtaagaa gtatgcattt tccaaaagac cacgaagaaa accatcaagc caaacgacgt
    55681 gtcgtttttg aagagttctt tttatttcaa atgcgtcttc aaggattgaa aaaagcagaa
    55741 aaagcagaaa cgaatggttt ggagatcctt tatgatgtcc aaaaattgaa acaatttact
    55801 caaaagctac cttttgaact gacaaatgca caaaaacgtg taacgaatga aatctgtcgg
    55861 gatcttcgca gtccgcagca tatgcagcga cttttgcagg gagatgtagg tagcggaaaa
    55921 acagtggttg ccgcgattgc gttatacgct accgttacag caggtttcca aggtgcgttg
    55981 atggtgccta cagaaatctt ggcacaacaa catatggaaa gcttgcaaca gctgtttgat
    56041 cccaatgaag taaccatcgc attgattacg gggtcaacga aagcaaaaga acgacgtgag
    56101 ctccttgaaa aactagagca aggggaaatt aacgtcgtga tcggcacaca tgcattgatc
    56161 caagatggag tcgaatttca gaatttaggt ctagttatta cggatgagca gcaccgtttt
    56221 ggcgtgaacc agcggaaagt acttcgtgaa aaaggctggc gaccagatgt cttatttatg
    56281 acagcgactc cgattcctag aacattagct attacagcat atggtgagat ggatgtatct
    56341 atcattgatg aacttccagc tggacgtatc ccgatcgaaa cacgctgggt aagaacacct
    56401 caattggaca gtgtactgga ttggacgaaa aaagaactgg caagaggaca tcaaatgtat
    56461 gtcatctgtc cgctgattga agaatcagaa gcattagatg taaaaaatgc agtggaaatc
    56521 tatgaaaaac ttcgtgaact atttgcacca aaatatgaag ttggcttgtt acatggtaaa
    56581 atgaagaatc aggaaaaaga agtaatcatg gaagccttca aagacaacca gatgcagata
    56641 ttagtttcga ctactgtaat tgaagtaggg gtaaacgtgc cgaacgcgac cgtcatgctg
    56701 atcatggatg cggatcgatt tggattggca cagctgcacc aacttcgtgg acgtgttgga
    56761 cgcggaagtg atgcttccta ctgtatctta gttgctaatc ctaaaaatga actaggtgtg
    56821 gaacggatga aaatcatgac agagaccacc aatggttttg tactaagcga aaaagatctg
    56881 gaattacgtg gtcctggtga agtatttggc ttccgtcaat ctggtctgcc tcaatttgcg
    56941 attgctgact tggtgacgga tggaaatgtt ttggaggtag ccagagaaga ggctgcagcc
    57001 ctttggcaaa taaaggattg gcagatgctt cctgagtatc agcctttagc gcaaagcctc
    57061 caaatagaaa atcaggataa tcgttatttc gattagcaga ctgtgctata ctttatactg
    57121 atgacggaaa aaggagtgga aacaatgaaa attgccgtag atgcaatggg cggagatcac
    57181 gcacctcaag caattgtaga aggcgtggct ttagcgcagc aagattttcc agaagtagaa
    57241 ttcttacttt acggaaaaga agcagaaatc aagaaatact taacaaatga aaaaaacatc
    57301 acgattatcc atacagacga aaaaatcaat agtgatgatg agccagtaaa agcaattcga
    57361 cgaaaaaaga ctgcatcaat ggtccttgct gcacaagcag tgaaaaacgg tgaagcagat
    57421 gctgtatttt cagcagggaa tacaggcgcg ctactagcag cagggttgtt catcgtagga
    57481 cgtatcaaaa atatcgaacg ccctggcttg atgtctacct tgccagtagt agggaaagaa
    57541 ggtgccggtt tcgatatgct ggatcttggc gctaacgcag aaaacaaacc agaacatttg
    57601 ttgcaatacg gtattttagg ttctttttat gcacgtaaag tccgaggtat cggacgtcct
    57661 cgtgtcgcac tcttaaacaa cggaacagaa gcaacaaaag gtagtgaagt gacgaaaaaa
    57721 gcctatgaac tccttcagaa tgaaacaagc ttgaatttta ttggaaacgt agaagctcgt
    57781 gagctactca atggtgtggc tgatgtagtg gtcacagatg gatttacagg caacgctgtt
    57841 ttgaaatcta ttgaaggaac agcaatgaat atgatgaatc tattaaaatc tgctattcta
    57901 aatgaaggga tcaaaggaaa aatgggcgct atgcttttaa aagacggctt acgttctttg
    57961 aaagcagaaa tggattattc caaacatggc ggtgccgttt tgtttggctt gaaagcacca
    58021 gtcattaaaa ctcatggagc aacaggcccc gatgctgttc gctatacgat ccgccaaatc
    58081 cacacaatgc ttgagacaga tgtagttgga caattagtcg aacaatttga aaaaagcgaa
    58141 gaatagtatt tggatatctg aagcgatccg ttccattgaa aaaaaggtgg acattcctga
    58201 aaaatgccag taaaattagc catggtgtag gtaattgaca agtgtatgat tcttggaggt
    58261 gaaaaagttg actcgcgaag aagtatttaa caaagtagcg aaaattattt caaatcattt
    58321 tgagatagat accgataaag tgacggatga attgaacatc aaagatgatt taaaagctga
    58381 ttcaattagt attatggaat tcgttttaga gcttgaagat gaattcggta cggaaatttc
    58441 agatgaagac gctgaacaaa ttgaaacagt cggtggcgca gtagattata tcagcagcca
    58501 tcttaaataa tttcaaataa aagcaaagaa ggataaaaag ctgcatgcag ctttttatcc
    58561 ttcttttttt aatctttttg acacgtaaaa gtcatttttc tgataattct ggcgataaac
    58621 agaaattatg ttgcaaagta aagcggtttt ctatataatt attaataatt cattacacaa
    58681 agataagaat aacctttgtt ataaaacgat ttattctgtg taatgtcctt ttttattaag
    58741 aaaaacttcc atttaggaag cagagagggg tattcgattg tccgataacc aattattaga
    58801 agttcagaac ctacatacag gattccgact caaagatgac tattacgatg cagtagatga
    58861 cgtttcattt actttagaca aaaacgagat tttagcaatc gttggggaat ctgggtgcgg
    58921 gaaaagtacc ttggctacta cgattatggg attacttgat cctaataaca caaaaattac
    58981 aggtgagatt ttatataacg atctgaattt aatcgacctg aacgaaacgc tctataataa
    59041 aatccgcgga aatgatatcg gaatgatttt ccaagatccg ttgtctgcat taaatcctct
    59101 catgagaatc gaagaccaga tcaaagaagg actttcctat cacacaaaga tgaatgcaga
    59161 acagcgtcaa gctcgtgcac tagaattgct agaacaagta gggattccta atcctgcgag
    59221 agttggacgc caatatcccc atgaactatc aggcggtatg agacaacgtg tcatcatcgc
    59281 aatcgcaatc gcctgcaaac ctccaatcgt cattgcagat gaaccgacga cagccctaga
    59341 tgtaaccatc caagcacaga ttcttgattt actgaaagac cttcaggaag aaacaaaaac
    59401 agggatcatc ttgatcactc atgacttagg agtcgtagca gaaatggctg atcgagtggc
    59461 agtgatgtat ggcggacagt tcgttgaagt agcaggtgta aaagaactgt ttgatcatcc
    59521 taagcatcct tatacacgct ctttactgaa ctctatacca caagaaggaa atcatgatgc
    59581 agaactccat gtgatcgaag gtgtcgttcc ttctctgaaa aacatgcctc gtaaaggttg
    59641 tcggtttgca ccacgtattc catggatacc agcaagcgat catgaagaag agcctatcct
    59701 tcatgaagtc gcaccgaacc acttggtacg ttgctcctgc tataagcatt tccactttag
    59761 agacgaaaaa ggagaggtgt agatgacaga attaatcaca atcaaagacc tgaaagttca
    59821 ttaccctatt cgcagtggat tcttcaatcg agtaacggat catgtatatg ctgtagatgg
    59881 tgtggatttt attatcgaaa aaggaaagac atatggttta gtgggagagt ctggctctgg
    59941 aaaatctact acgggaaaag ccgttgtggg attagaaaag gtaacagccg gacaaatcat
    60001 gtatgaagga aaagatgtaa cgaagcgaag caaccgcaaa aaaatgggtt ataacaagga
    60061 tgtgcagatg atcttccaag attcaatgtc cagtttgaat cctaaaaagc gagtgttgga
    60121 tatcattgct gaacccatcc gaaattttga acggttaagc gatcaagaag aaaagaaaaa
    60181 ggtaaaagga ctgctagata tcgttgggat gccggaagat gcgctatata aatatcctca
    60241 tgaattttca ggcggacaac gtcaacgtct aggagtagcc cgtgctgttg ctaccaatcc
    60301 aaaactgatc gtagccgatg aacctgtatc tgctctggat ctatctgttc aagcgcaagt
    60361 attgaatttc atgaaacgaa tccagcaaga atacggcctc agctatttat tcatttcaca
    60421 tgatttaggt gtagtaaaac acatgtgcga caatatcgca attatgtaca aaggtcgatt
    60481 tgtagaaatc ggtacaagag aagacattta taatgacccg cgacatatct atacgaaacg
    60541 tctgctttca gctattccgc aaatcgatgt agaaaatagg gaaagccaca aagaaaatcg
    60601 acgaagagta gaacaagagt atatccaaaa ccaaaaagaa tactatgatg cgactggtcg
    60661 agtctacgat ttgcgtacga tcacaactac acacaaagtg gcgttgaaag atggaggtgc
    60721 aagctaatgt ggaaaacgat tctgcgacgt gttttattga tgatcccgca agttattatt
    60781 ttaagtgttc tgatcttctt attagcccaa gcgatgccgg gggatccatt taccggtttg
    60841 atcaatccaa atcaggatcc ggcagtgatc gaacagatgc gacaagcagc tggcttgaat
    60901 gacccatggt atgtccagta cttccgttgg atcggaaatg ctttgcaagg cgattttggt
    60961 caaagtttct tatacaaacg ctctgttgct gacttgatcg gcgaacggat catcaacact
    61021 ttatatctat cgatcttgac agttgtcctt acttatttga ttgctgtgcc tctggggatg
    61081 cttgctggcc ggtatcaaaa ttcaatcttg gataagattg tcgtaatcta caacttcttt
    61141 agttttgctg ttcctttatt tatcttcggc ttgatcatgc tatttgtttt tggttatcgt
    61201 ttaggctggt tcccaacaag tggatcacaa acactaggtt tgcaaggtgg atttatcact
    61261 caatggatag accgtttgaa atatattctg ttaccatcaa ttacccaagc atttttggcg
    61321 actgccgtca caatccaata tctgcgaagt gaagtaatcg attcgaaatc gctcgatttt
    61381 gtcagaacag cccgttccaa aggggtaccg acgaataaag tattcagtcg acatattttc
    61441 cgaaatgcag cgttgccgat ggcttctcaa atggggtatg aaatcacttc gttgattgct
    61501 ggttctgtag ttatcgaaaa gatttttggt tatccgggag tcggaaaact atttattgat
    61561 tccatcggcc aacgggacta taccgtgatc acatccttag tattgatttt aggaatcgcg
    61621 acgctagtgg ggacattact ttcagatatc atcatgagta tcgttgaccc gcgtattcgg
    61681 attcaataaa accaatcaga aaggagaatg gatcgatgag tgaaaaaaac aatacagcag
    61741 ttcaagaaag tattcctcct atggggatac gaatgattgc tcgagaattt aaaaaagata
    61801 aaattgctat tttttcatta ggtttgctag tggttttatt attagtgatc tttatcggtg
    61861 cgatgctgac cgatcaagac aaagtgatgt atgttagtat cttcgacaaa tacgctgcac
    61921 caggaacagt ttcgacacaa ggacaaaagt tcattctggg agcagatgaa ggcggacgtg
    61981 atgtattagg acaattgatc ataggtgcta gaaactctgt tacgatcgga tttgcgatca
    62041 cgatccttac ttcgatcatc ggggttggat tagggatcat ctctggctac tacggaggga
    62101 tcattgacaa catcttgatg cggatcgttg attttatcat gattctaccg atcatgctga
    62161 tcattatcgt cttcgtatcg attatttcca gatacaatgt ctggtccttt atattcatca
    62221 tgagtgcatt ttattgggta gctaaagccc gattattccg aagcaagaca ttatctgaag
    62281 caagaagaga ttatgtcagt gcctcaaaaa caatgggaac caatgatttt aaaatcatgt
    62341 tccgcgaaat catgccgaat ctaagttcat taatcattac taatttgacg atgaactttg
    62401 cggcaaacat cggtatcgag acaactttga ctttcttagg tttcggcttg ccggcaaatg
    62461 tacctagttt aggtacattg atcggttatg caagtaacgg agaagtctta gtgaatcggc
    62521 aatggatctg gttacccgca tcaatcctta ttttagtttt gatgttgagt atcaattatg
    62581 ttggacaagc attcaagcgt tctgcagatg cgcgccaacg gttaggctag tatagaagcg
    62641 aagggggaaa taggaatgaa gaaaacgatt tttggatttg cagcgctgat gtctgctgtc
    62701 gttcttgcag catgcggggg cggaggtaca gatacctctg gaaacaactc tggaggcaac
    62761 ggcaataaca aagaggaatt aacttttcca attgcaacaa aaaataatga tgatgcaatc
    62821 gaagacggcg aattagaagc tgcggtcgca acggacagtc agtttaaagg attgttccag
    62881 tgggagtttt accaagattc atatgatgca gcttttatgg ctccttctca tgaaccgcta
    62941 tttgcaaacg atgacgattt cagaatcaca gacgacggag ctgcaacgct tgatctagat
    63001 gaagatacga aaaaagcgac aatcaaagta aaagacaacg tcaaatggtc agatggtgaa
    63061 gatgtaacgg cggacgatgt gattttccct tacgaaatca ttgggaataa agattacact
    63121 ggtatccgtt atgacgacac tttccgtaac atcgtaggta tggaagaata tcatgatgga
    63181 agtgccgaca caatctccgg aatcaaaaaa atagacgaca aaacagttga aatcgaatat
    63241 aaagaagtaa acccaagcat gcttcaatcg ggtggtggta tctggtcata tgcagcacca
    63301 aaacatacgt tagaaggtgt agcaatcaaa gatatggaat caagcgatca agtacgtaaa
    63361 aatccagtga cttttggccc ttattacatg agtaatatcg ttgcaggaga atcagtagaa
    63421 tacttgccaa acgagcacta ctggaatggt acgcctaaat tgaaaaagat cacgatgaaa
    63481 tctgtaccga tggcaagtgc aactgaagca ttgaactcta aattatatga tatgattttc
    63541 caaatgccaa cagatactta cgatacctat aaagaaattg ctggctatac aaacttaggc
    63601 cgtgaacaaa cttcttatac ttatctagga ttcaagttag gtaaatggaa tgcagataca
    63661 aagagtgtgg aatacaatcc agattcaaaa atggctgata aatcattacg tcaagcaatg
    63721 ggctatgcat tagataataa tgcagtaggt gaacgtttct atgcaggttt gagaagcaat
    63781 gcgacttcat tgattcctcc agtattccag tcataccatg atgatgagat gaaaggattc
    63841 acacaagatc tagacaaagc gaaaaaacta ttagatgatg ctggctacaa agatgtggat
    63901 gacgatggtt taagagaaga caaagatgga aataaactaa caatcaactt tgcttcgatg
    63961 tccggtgggg aaacagcgca accattggca gattactata tccaacaatg gaaagaaatt
    64021 ggtttggatg tccaatacac aacaggtcgt ctgatcgaat tcaattcttt ctatgataaa
    64081 ttagaaaacg atgatcctga aattgatgtt taccaagctg cttggaatac aggaactgat
    64141 ccaaatccaa gtggactatg gggaccaaat gctgcgttca actacactcg ttatgaatca
    64201 gaagaaaata caaaattgat caacgatatc agttcaagca aagcatttga tgaagagtac
    64261 caaaaagaag catatcaaaa atggcaacaa tatgcatttg aagaagcatt tgcgattcca
    64321 acgttgttcc gtaatgaagt attgccagtc aacgaccgtg tcaaagactg ggattggtct
    64381 tatgatgtac caaacccatg ggcaaatgtt tctgttacag cagattcacg ttcataatca
    64441 ctaaaaatga ccgaaaaagg gcgatgtcct ttttcggttt tttgatattt ttctgtttat
    64501 ttctctaata ttcaggtcaa ggcgctgatt tcatgacttc tccaataaac ggtgttcaaa
    64561 gtccagtttc tcggtattaa gtcaaattta tgcaaaaata tggaaagcca ttttcacaaa
    64621 attttttctt catactcgaa actaaacgaa tttttcacaa cttctcgtca atcactagga
    64681 ttattaaagc tttacttacc ttttgaccga accatttcaa atttgacaaa aaaccagtat
    64741 aataaggaag tatcgaaaaa atatccataa aaggaatgtg gaaaatggac aaccagctga
    64801 caaaagaact aaaagaaaac tataacatcg tttttcacga tctaaatttg cttgaacaag
    64861 cttttaccca ttcatcctat gtgaatgagc atcgcaatct gcaattatcg gataatgagc
    64921 gacttgaatt tttaggagat gccgttttag aactgatggt ttctcagtat ttattccgtt
    64981 tatatcctca tttgccagaa ggaaaattga caaaaacacg agctgcgatc gttcgcgaag
    65041 acagcttgtc gaaatttgcc aaagaatgtc actttgatca atatgttttg ttgggcaagg
    65101 gagaagagaa ttcaggagga cgtacgcgac cagcattgct ttgcgaccta ttcgaagctt
    65161 ttttaggcgc gttatatctt gaccagggat tcgacacgac ccatgcattc atcgaaaaag
    65221 tgatttttcc taaagtcaaa gcaggtgctt tttcacatga gatggatcat aaaacaaaac
    65281 tacaagaagt cttgcaaaaa tcaggtgatg tatcaatcga ataccgattg atcaatgaag
    65341 aaggtccagc tcatgaacga gtattttgga tcgaagttta tgtagatgat caattgattg
    65401 gcactggaca agggaaatcg aaaaaattag cagaacaagc tgcggcggaa aatgcattgg
    65461 cagcactgta aaagtaagtg agggaaagct ttgtatttga aacgaattga aatagccggt
    65521 tttaaatcat ttgcagacaa gacagtcatt gattttgaaa atagcgtcac agcagtggtt
    65581 ggaccaaatg gcagcggaaa aagcaatatt acagaggcta tccgctgggt attaggagaa
    65641 caatctgcta aaagtctgcg aggcggaaaa atgccggata tcatctttgc cggttccgat
    65701 acccgtaagc agctgaatat tgcagaagtc acagtgattt tagacaatag tgatcattat
    65761 ctgccattgg agtacaatga gatcagtgta acccgccgtt atcgacggac tggggaaagc
    65821 gagtttttca tcaataaaca aagctgcaga ttaaaagata tccaagaatt gttcttagat
    65881 tctggattag gaaaagaatc gttctcaatc atttctcaag gaaaagtaga agcgattttc
    65941 agtagtaaac cggaagaccg tcgtgggatc tttgaagaag cagctggtgt cttgaaatac
    66001 aagcagcgga aaaagaaagc agaacagaag ctttttgaaa cagaagacaa cctcagccgc
    66061 gtccaagaca tcatccacga attggaagaa cagttaactc ctttggccgc ccagagtgag
    66121 gcagcaaagg agtttttacg gctaaaagaa acactgactc aaaccgatgt ctctttgatg
    66181 gttgctgaaa tcaagacagc aaaaaaagat tgggacaaca aacaagcaca gcttgcgaaa
    66241 ttcaatctag aattaggcaa actatctgag tcgatccaag aacaagaatc cattttggcg
    66301 aagcagcgta aagaaaatgc gcaagccgat cgtctgatcg aaaaaaatca gcaagtatta
    66361 ttagatcttt cggaaaaact gaagcagaca gaaggacaaa aagatgtttt gcaggagcgg
    66421 acaaagcata cacaaaaaag ctcgcaagaa tatcaaactt cattagcaga agcacaaaaa
    66481 aaggttaaac attttgaaaa gcttcaagaa agcttgatga aagcagcagc agaaaaagaa
    66541 acagagatcc aaaaagctga agcaaatctc ataaaaacac agcaagaatt agaaaaatac
    66601 caaaaatcaa cgaaagaatt attggcagaa ttaagagatc aatatgttga tttgatgcaa
    66661 gaacaagcag ctgtaggaaa cgaactgaag tatctagaac gtcaatatat acaagaaaca
    66721 gcaaaaagca agcagacgtt agccaagcaa tcggaagtag aagcatcagt agaccgattg
    66781 atcttacaaa aagaagaatt gacacaaaag caggcgcagc taaaatcatc tttaactgaa
    66841 acaaaagaga agttagaaat gatccaacaa aacgggaaaa agttccaaga agcattagca
    66901 aaagaacagc ctaaaatgta tcaattaatg aatcaagtcc agcagctacg tgctcgtaag
    66961 aaaagccttc aagaaatcca agaaaactat tttggttttt accaaggggt tcgattggtc
    67021 ttgcagcata aacagcagct ttcgggtatt gtcggagcag tggctgaact gatcgatgtt
    67081 cctgcagatt tcacgttagc tatcgaaaca gctttaggtg gggcagccca gcatgtcatc
    67141 gttgaaaatg aaaaagatgc tcggcaagcc atcacgtatt tgaaacagca aagaggagga
    67201 agagcaacat tccttccact gacgacaatc aaaccaagac aattaccggc tcatatattg
    67261 acacaagcag cagctgtaga agggtttata gggattgcca gtgaacaagt cagttatccc
    67321 gatcaaatcc aaactgtggt tcaaaacctg ctaggaacga tccttttagc caaagactta
    67381 actagtgcca atgcaatcgc gcaaacgatc cgctatcaat atcgagtggt ttccttagaa
    67441 ggtgatgtga tgaatgccgg aggatccatg acaggtggag cgaataaacg aggaaaccaa
    67501 ggcagtttgt ttgtacaaaa tcaagaattg aaacagttga ctagtgaatt tgaagaagca
    67561 gataaacaac tccaagcaca agaaaaaaaa gttcaggagc tgcaacaaga aacagctcgt
    67621 ttagccgaag aacaagaagt tttgcgtaca cgaggcgaac aattacgttt tgaagaacaa
    67681 gaagcgacaa atcagctaca aaacatcata aatgaacttg aacgttttga aaaagaaaag
    67741 caaatctcaa cttttgagac acgtgaactg cagcagttta tcgaagatta cgaaaagcaa
    67801 acgaacgaat tgaaagacaa acaaacagat cttgaatcgc aacgacaaaa aatcgacgaa
    67861 gaaatcaaat cgttaagtca agaaagcgac cagatggaag ccagaagagc acaagtacaa
    67921 agtcaaaaag cacaagaaca agcagatctt gctgtcttga aagagcagtt taatcatctg
    67981 cagatccagc tccgcggtgc ccgagtccaa aaagcagagg caaccgaacg tcaagaagcg
    68041 atagaaaagc agttggcgac attgacagca gacttttctg atcatgaagt cacagaagaa
    68101 agtttggaaa aacaaattaa cgaattatct gctcagcgag aaacattaaa agccgagttg
    68161 gcaaaagcca aagagcagcg agatagaagg caaaaggaga tcgataagct tgaagcagtc
    68221 ttggcagagc gtaatcgtga gcaaaaagca cgactgtcgg aacaatccaa attagaagtc
    68281 caaaaagacc gagctgaaat gattttagat aatcatttga cctatctgca gtcagaatat
    68341 cagctgacat ttgagaaagc ttcccaagac tatcaagaaa caaccgatat cgaagacagt
    68401 agaacaaagg tttcatcctt aaaagaacag attgaaaaac tgggaccagt aaacttaaat
    68461 tcaatcgaac aatacgaaca agtcagtgaa agacatacat tcttagcaac acagcgagat
    68521 gatctgttag cagcgaaaaa ccaattgttt gaaacgatgg atgagatgga tgatgaagtg
    68581 cggacaagat tcaaagaagt atttgaagct atccgtcaag aattcaaaat tgtctttcca
    68641 aatatgtttg gcggcggtaa agctgaatta gtactgacgg aaccaactga tttgctgaaa
    68701 acaggtattg agattgaagt acaacctcct ggcaaaaaac tgcaaagtct aagtttgctt
    68761 tctggaggag aacgagcact tacagccatc gcgttgctct tttccattat ccgtgtctgt
    68821 cctgttccgt tttgtatctt agatgaggta gaagcagcgt tagatgaagc caacgtgaaa
    68881 cgttttggtc gttacttgag cgatttccaa gatgacacac aatttatcgt agtcacacac
    68941 cgcaaaggaa cgatggtcgc agcagatgta ctgtacggag tgacgatgca ggaatccggt
    69001 gtatcaaaaa tcgtttctgt acggatggaa gatatcaatg aagaggcgaa aatcaacgaa
    69061 acgaaagtgt aggaacgaaa atgatcaaat tagtagcaat cgatttggac ggtacattat
    69121 taaatgaaca aaagctgatt tcagatgaga ataaacaagc attagcacaa gcaaaacagc
    69181 aaggggtcaa gatcgttcta tgtacaggac gccccttagc tgcgatggcg cattatctgc
    69241 aagaattagg actggtagat gaaggtgact acagcatcac tttcaacggc ggtttggtac
    69301 aaaaaaatga tactggtgaa atcatggaaa agaaagtgat ggaagtatca gatatccaac
    69361 gcttatacaa actggctcag caattggatc tgccactaga tgttttgtct gacaatgtgg
    69421 ttttacagct gcctagtgcc ccaaggaagc aatccttgta caatgtgttg aataagctgc
    69481 tgcaattcca accagcggat ataagcgata ttacagatga atttgtgctt aataaggcag
    69541 taatcgctta tgaccaagaa gaattagatc ctaaaatcaa agaaatacca gcagaattcc
    69601 atgaaatgta tgagatcatc aaaacaagaa gtatgctttt ggaatttatg ccaaaaggtg
    69661 ttacaaaagc ttatggtatt tctttattgg caaaagatct tggtttgaaa caagaagaaa
    69721 tcatggcgat cggtgatgag gaaaatgatt taccgatgat ccaatatgct ggactaggcg
    69781 tagcgatgga gaatgctgtg cctttcgtca aagaagcagc agatttcgtg acagccagca
    69841 atgaagaaaa tggcgtagcc aaagtgatcc aaaaatttgt attagagtag ggggagtcaa
    69901 atgggatttt ttgataaaat caagcgtgct tttactggcg aagagaaaac agaagaagtt
    69961 gaagtacagg agaagtacga caagggttta gaaaagacgc gtaaaacatt tggtgaacgg
    70021 atgaatgagt tgtttgccaa ttttcgaaca gtcgatgaag acttttttga agaattagaa
    70081 gaaacactta tcggtgcaga tgtaggtttt gaaacagcga tacgaattac cgaagcatta
    70141 cgtcaagaag tgaaactcag aaatgcgaaa aaatcggcag aagtccaaaa tgcgatcatc
    70201 gagaaacttg tggatctcta tgaagcggaa ggaatcaatg aagtaaatga actaaatatc
    70261 caagaagacg gactaacagt tattttgttt gtcggtgtta atggtgtagg gaaaacaaca
    70321 agtattggta aacttgccca tcagttcaaa aacgaaggta aaaaagtatt gatggcagca
    70381 gcagatacct tccgcgctgg agcaatcgac cagttagttg tctggggcga acgcgctggt
    70441 gttgaagttg ttcgtggcaa tgcaggaggc gatccagcag ctgtcgtatt cgatgctttg
    70501 gaaagagcaa agacagagca ggcagatgtt ttactagttg atactgcagg ccgactgcaa
    70561 aataaagtga acttgatgaa cgaattagaa aaaataaaac gagtaatcca gcgggaatta
    70621 ccagaagcac cccaagaaac acttcttgta ttagatgcaa ccactggtca aaacgcgatg
    70681 gttcaagcaa aacaattcaa agaaacaaca gatgtgaccg gtcttgtttt gacaaaatta
    70741 gatggtacag caaagggcgg tatcgtactt gctatccgta atgaattgca tttaccagtg
    70801 aaattggtcg gtcttggtga aggaatcgat gacttggagc cgtttgatcc aaatgatttt
    70861 gtcgttggat tgttcaaagg attactaaaa gaagaataac ccatgtctaa aaagcaggag
    70921 ctgtgacaaa agttgcagct ccttgtttga atgattgttt ttaacatagc tgtgtttttg
    70981 atgccttcat ttttatactt atttgtaagc ggttctcaaa caatttcgtt ctttttctta
    71041 gactttttga ttataattag attcatatga attggaggtc acttaatgat gaattttaag
    71101 caattattat tgcatgtcaa tgcgcgccct tttatcgacc aagctcgatt tggtatagaa
    71161 cgggaaggac agcgggttga tcttgcagga aatctagcaa aaaccgatca tccagcaatc
    71221 tttggcgatc gatcctatca cccctatatc caaacagatt ttagcgaaac acaaacagag
    71281 atgatcaccc ctgttaccga ttctattccc gaattatttc agtatctcgc tgctgtttat
    71341 gatgtgactg ctcgttctat accgaaagaa gagatgatct ggccattaag tatgccacct
    71401 gccttaccgg aaaaagacga agagatcatt attgcaaaat taaaaaattt cgaagatgtc
    71461 ttgtatcgac gttatttagc aaaagaatac gggaagcgaa aacagatggt aagcggtatt
    71521 catttcaatt ttgaatttgg cgatgagttg ctaagaacat tgttcagcca tcaggaagaa
    71581 tttcaagatt tttccgaatt caaaacagaa ctttatttga aaacagcgag aaactttatg
    71641 cgctatcgct ggatgatcac ttatttattc ggtgcttcac caatgagtga gaagaactac
    71701 tttttagatg aatcacatcc gcaagaacct gttcgcagta ttcgcaacag tgcgctgggc
    71761 tatacgaatc atccaaatgt gaaagtttcc tatgcttcta tgaaacagta tttagcagat
    71821 atcgagcgaa tgatcgaaga agggaaactt tcggaggaga aagaatttta tacaccgctt
    71881 cgtttccgag gcggaaagaa agtcgcagat ttagcaacaa caggtgttcg ttatattgaa
    71941 ttgcggaata tcgatttgaa tccttatgca agattgggta tcaatccaga gcaagttcgg
    72001 ttcttacaat tattcctgat gtacatgtta tggacagaag aaaaagaaga ctgtgaccaa
    72061 tgggtagcag aaggtacgac tcgaaataac aaagtagcac tagaacaacc atcagatcaa
    72121 acagaatttc atcaagaagg tagagaaatt cttgaaggaa tgaagcaaat gctggttgaa
    72181 ttggattggt tggattctct ttatttagta gaggaagcat tgactcaaat ggatcatcct
    72241 gaacaaacgt tagcagcgaa actctatcaa gaagcacagc tatcgagtca gcaagaagtt
    72301 gctgtggcat tgggacatca atattataaa gaaagccatg aacgtcccta tcagttggcc
    72361 ggttttcgcg aaatggaact ttctactcag atatttatgt tcgatgccat ccaaaaaggt
    72421 gtccaagtca aagtattaga tgagtcggat caatttttgc gtctgcaatt ccaagaccat
    72481 gtagaatatg tgaaaaatgc aaacatgaca agtaaggaca gctatatcgt cccacttatc
    72541 atggaaaaca aaacagtaac gaaaaaagtc ttaaaagaag ctgggttccg ggtaccagga
    72601 ggcgcagaat tttcatccat ggaagaagcg gtaaaagctt atccgagatt tgccgagcag
    72661 gcatttgtga tcaaacctaa atcaaccaat tacgggttag gtatcacgat tttcaaggaa
    72721 ggagctagtc tggaagatta tcaagcaggt ttagccattg cttttcgtga agacagttcg
    72781 gttttagtag aagaatttat gccgggaacg gaatatcgtt tctttgtgat cgacggagaa
    72841 gtccaagcca tcatgttgcg agtccctgct aatgttattg gtgacagtat ccgaacagtg
    72901 aaagaacttg ttgaagaaaa aaatagtgac cctttgcgtg gaacgaatca tcgtgcacct
    72961 ttagaattga tccaattagg tgaattagaa caattgatgt tgaaagaaca agggttgacg
    73021 atcgaatcag ttccccaagc caatcaaatt gtttatctaa gggagaactc aaatatcagt
    73081 acaggtggtg attcgatcga tatgactgat gaattttctg aagcttacaa aaaaatcgcc
    73141 gtttctgcgg tagaagcact aggagctaag attagtggta tcgatttgat tataccagat
    73201 aaagaaatcg atcctacaac tgataaaaaa gcatatggga tcatcgaagc aaatttcaat
    73261 ccagccatgc atatgcatgt ttatcctttt gcaggaaaag gcagaagatt gacgatgaat
    73321 gtattgaaat tgttatatcc tgaagtattt taaaagaaaa agcttttgac agattgtcct
    73381 atttgatgtc aatctgatca aaagcttttt ttgcatgtaa aaatttatgt gttttttcgt
    73441 tttaaaataa acgatcccag aagctttttt tcttttttgg tttttctttt tcagctggcg
    73501 aagagttttc ttctgggtat tttttttcga tatcaatcac aggttcattt actgcttctt
    73561 ttgctgttaa gagcaatccg aattcctcag gatttgcagt gacgtaatcg ttgactacag
    73621 tgaaacggct attgtttttc gttgctagct ggatatattg gttttggaca gaaaggggta
    73681 gagccccatt caagagtatg gaagcatctg gatgtttttt gagttctttt aaaaagtttt
    73741 ctttgttttt tggttggatc atttcagcaa cagtcatact aagatagcag cgttccctga
    73801 atgtccctaa atatttatgc tgttcttcag ggttcactaa aggtgtacca taaagtccct
    73861 tatccaaatg gctttttaca tcatcttgtg ccataaaaaa taccctcctt cttctctttg
    73921 tctttcttac gattatagca tattcccaat aatacctaaa acatatgaaa aagcggttgc
    73981 ttttgttcaa cgattcactt aaaataagaa tgaaaatcat tctcaattag aaaggtggat
    74041 tggatgcgct acggacgact agtagcatta ttagttattt taagtagtat ttcattgttc
    74101 atcggagtat cgaatgtaca attgtcagaa ctgctccagt tagatgggca aagcttacaa
    74161 atcttgctag tgactcgttt gccacgaacg atttgtttga tccttgtggg agcgaccagt
    74221 agtatttgtg ggttgatcat gcaacatttg acccaaaata aatttgtttc accaacgact
    74281 gcgggcacga tggatagtgc gcgtttaggt attttagtgg ctatgatttt cctacctggt
    74341 gcatccttga ttgtccgatc cttgacagca ttttgctttg cttttgcagg aacattgctt
    74401 tttttatccc ttacgcgatt gttcctccag aaaaatcaat tgatgcttcc gttgattgga
    74461 gtcatgttag ggaatatcat tggttcagtt gctacttttt ttgcttatca attccagttg
    74521 gtacaaaata tgacttcatg gcttcaaggg aattttgcga ctgtgatacg aggaaattat
    74581 gagttattgt atgctgttgt tccattgttg ctcttggttt ggctatttgc ttaccgattt
    74641 acgatcattg gtctgggcga atcgtttgca gtaaatctgg gaatagatta ccagaagatg
    74701 cagtttttag gcattggctt agtatcattg gctagtgcag tcatgttgat catggtagga
    74761 agcattcctt ttatcggagt gatcattccg aatcttgtct ctagaatata tggagatcaa
    74821 gtgcacaaaa caattggcgt aactgcttta gcaggtagtt tgttcttagt attttgcgat
    74881 cttgtcgctc gcgtgttgat ttttccttat gaaataccag tcagtgtgat cgttggaatc
    74941 atcggtgggg ctatcttcct ttatcttctg ataaggggac gacgatatgc ttaaaaaaaa
    75001 gccatctatt tttattggta tgcttgttct tgttttattt gttacaggaa tgtacttgac
    75061 gatccataca tatggaaact ggcattttgc atgtgcattg aggagtaaga aaatttttgc
    75121 atttatcctt gtctcgctag ctgtcagcag tgccacaatc agctttcaga caattacgca
    75181 taaccagttt ttgactcctt cagtattggg acttgagtcg ttatatgtcc ttgtccaaac
    75241 ggtccttttc ttctttatcg gaggagtgac gatgcttcag cagacaggta cagttttttt
    75301 cttattgaac gttgggctga tggtcgggct gagtctattg ttatttttct ttcttttgaa
    75361 aaaaatgaga gcgaatcttt ttttattact gatgtcaggg atgattttag gtacgctttt
    75421 tggtagtgtc agtacctttt tacaagtcat catggatccg aatgaatatg atttgcttca
    75481 aggcagatta tttgcaagct ttggcaatat ccagagtcag tatcttttgc ctgcttcttt
    75541 gatcattatc ggctgctggt acctgttatt tcgatatcga catgtgctag acgttttcca
    75601 tctcggaaat gaacaagcag taaacctcgg gatagacaca gatcgttttg aatttttgat
    75661 tttatgttgt gtcagtcttt tgacagggac atcaacggct cttgtgggtc cagttacttt
    75721 tttagggttt attgtcgcaa atatcagtta tcgtgtcttt tctacttatc gtcatggtca
    75781 gctatttatc ggaagctttc tggtagctat gctgtttctg acaggaggac agctcgcagt
    75841 agagcagatt ttcggctgga atacaacagt gagtgtcgtc gttgaatttg tcggaggaat
    75901 ttattttgtc acaaaattga ttcgcgaaag gaagagtatc ctatgattga tttacaaaag
    75961 gtcaataaac tgtaccatgg aaaatcagtg gttaaagatg tacagttaac gattcctgaa
    76021 aagaaattga ctgcttttat cggtccaaat ggtgctggaa agtcaacggt gttgtcgatg
    76081 atcagtcgtt tgataccaaa agatacaggt gaaatctatt tagaccataa cgaagtaaag
    76141 gcttggaaat ccaatgaatt agccaaaaaa ttatcgatcc tacgacaatc gaatgaaata
    76201 aatgtccaaa tcaccgtgaa agaattagta gaatttggac gatttcctta tacgaaagga
    76261 aaattgacga caaaagacga ggaaatcgtt caggaatcac tggaacacct tgggttagaa
    76321 gaaatagcgg atcaatcgat tgacactctt tcaggcggtc aactccaacg tgcgtatatt
    76381 gcaatggtgc tagcgcaaga cacggattat attttactgg atgaaccttt gaacaatcta
    76441 gatatgaatt ttgcggttca aatcatgcag attttaaaaa atttagtaca agaaatcggg
    76501 aaaacaattg ttattgttct tcatgatatc aactttgctg caagttatgc agacgaaatt
    76561 gttgcaatga aagatggaca gttatttacg caaggaacga cccaagaaat gataaaaaaa
    76621 gatgtattgg atgaattata cgaaatggac atacggattt gtgagattga aggccgcaga
    76681 ttctgtcttt atttctaaaa ctaacattag gagagaagag atgaaaaaat tagtagtagg
    76741 tatcttgatt ctagcatcat ttggtttagc agcatgtgga aatagcaaca acaccagtca
    76801 ggcagacaca aaaaaaagca gcacacaaac tgaaactaca ttgacgataa cagatagtaa
    76861 tggagaccaa atcgaggtgc caaataatcc agaaaaagtc gtcgtattcg acaatggttc
    76921 tttagatacg atggatgctc ttggtgttgg agataaggta gttggagcag ctacaagcag
    76981 ccttcctgag tatctttcat cctataaaaa agtagaatca gcaggtggaa taaaagaacc
    77041 agacttagaa aaaattaatc agcttcagcc agatctaatc atcatttctg gccgacaaag
    77101 agattttcaa aaagatctaa gtcaaattgc tccgaccatt ttcctttctt tagatgcgaa
    77161 aaatccatgg gaatctttcc agcaaaatgt cactgcttta ggggagatct ttggtaagca
    77221 agaagaagct aaaactcaat tagaggaatt atctagtgca atcgatcaga cgaaaaagaa
    77281 agcagaagct actgacaaaa aagcactagt cacacttgtg aatgaagggc aattatcagc
    77341 ctacggatct ggttctagat tcggtttcat ccatgattta tttggctttg aacaagcaga
    77401 tgatcaaatc gaagcatcca ctcacggtca aagtgtttct tatgaatatg tgctggaaaa
    77461 aaatccggat atcttatttg tagtagacag aacaaaagct attggtggag acgacagcaa
    77521 agatgatatt tctgccaatg aactggtagc acaaacgaat gctggtaaaa atcagcagat
    77581 catctccctt gaaccagatg tctggtattt aagcgggggc ggtttagaat caatgaaact
    77641 gatgatcgaa gatgtgaatc aagcatttaa atagcataaa taacagagga ttaaactctc
    77701 atactatgta acaataatgt ttcttacatt cttaaatagc aagagcctat cctcaaagaa
    77761 gttatcaaaa accatccgta aaatcgtatt gatgatttta cggatggttt ttatttattt
    77821 gagaaatctt ttttcaacgt gaattcatta tctacatcat acgggttgat tctagaatgg
    77881 ggagtggcaa aaaatagtgt gatccatttt ttcagcatca agatcgcctc ctttgatttg
    77941 aatcaaaggt acaaagcgaa acttagataa aacttaaatt tagatatttt tattttgtac
    78001 cgatctcttc tttaaacgat gttccatctg ctgcttctcg gcaataagtc cactagacta
    78061 gaaaatatga gaagctattt tctgtccagc gttcttatta ctcaaagcaa atcgcagttg
    78121 tcacaatctc ttctaaacga tgttccatct gctgcttctc ggcaataagc ccactagact
    78181 agaaaatatg agaagctatt ttctgtccag cgttcttatt actcaaagca aatcgcagtt
    78241 gtcacaatct cttctaaaca atgttcaatc agctgacctt cggtattagc tcaaagcact
    78301 gatttcacaa cctctattta ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc
    78361 atttttcgat aaaaatcggc gggaagctta ctccaccgtt ttaataattg atagaaataa
    78421 ctctcttctg ctttgatttt atactcgctt aatatcaatc caaaactatt cgtttttttt
    78481 gaagctaatt ttttataaat atccaatgct ttggttttat cttcattgac aaagagtgtt
    78541 tttacttcaa tataagaacc ctcgatattt tgtgtttctt cgataaaata gatgtttttt
    78601 atatattcct tcatagtgat tcctccaaag aagtctttta tttgataagt ttactctacc
    78661 gaagagttgt taataatccg tgaacaaaaa gtaattcttt ttccaatagt tgttaaaggt
    78721 tttttatttt ttatagtatt ttttgttatt taagaaagga ataaggttaa acaaataaca
    78781 ggttgctttt tgggtctttt tccgttcaag aaactgaacg aagaaaaggg aaagttgtct
    78841 cttttgtgat aagagaaatc ataataaacg aatgacttac tttccaataa attgagaaca
    78901 gtcagaacca gacaaattag ttgcatctga tgaataaagt gattattcta tgacatgagg
    78961 tgatacagat gaaattagaa gtgacaaaag aagcacaaga agtattgaag aataaattag
    79021 aagcagacga tcaactttta ttagatatcg aaaacggtga cggtccattt gcagaaagcc
    79081 aagtcagttg ccaattggat acggcatttc gattgatcat tgtcaaaaaa gatacacaaa
    79141 cagatctaag tctttattct gtagtggctg atacaccagt tggaccaatc aaaatcaaag
    79201 acagtgcaat actttacatg gatgatccat ccacacttgc tttagaacca acttataata
    79261 gtttacaatt aaaaggacca agcggcttga gaaaaggcaa cttgcaagtt gttcgaaaag
    79321 actagaggga taaaatggga aaattggcga tcatcagcga cctacatgtg gacatcaata
    79381 aattcggcga atcagagctg atgcagcttt ggcaagtttt gcaaaagcac aagatcactc
    79441 ggcttcatct agccggagat acggctaata aagtagaccg ttgcctagct gttgtcgaat
    79501 ttttcgtcaa taaaggatta tctacgacat ttcatttcgg caatcacgag ttggcagatg
    79561 tgcccaatga acagatgatg gaccattttc cagacccaca ttttttaaat gaacggtata
    79621 ttgctttaaa tgagcagacc gttcttttag ggatgaatgg ctggtacgac tatcagtttt
    79681 ccgaattgaa agattacgag gcaatcgtac gaatgaagcg tttgtattgg tatgatcggt
    79741 tcatccagcg tgggaaaaca gatctcgagg tcaatgcaga aatgcttgca gctgcaaaaa
    79801 atttgctaga ccagctggaa gcaaaaaaac ttgaggtgat tttatccact catttcgttc
    79861 ctaaacgggc atttatcatg tatcaatctg ctccttatga gcgttggaac aaattaaatg
    79921 catttttagg atcagaatca ttcggagaat tacttgaccg ttattcaaat atcaaacaag
    79981 tagtttttgg ccatacgcat cgtcgctttg aagataaatc catcggtcac acgatttaca
    80041 gctgcagacc ttttggttat tattatgaat ggcagctgac ccgagatttt gtcttgaaag
    80101 aacagttgct agacagctat gatcctatga aattacgtgt cctcttgcgt gcccatcacc
    80161 cagcttttca ggcctatcaa acacaacatt tgcaagagga atttgaacaa gcgatgacaa
    80221 tcgttccgta ttgagacagc attagtaatg tgtaaaagaa aataccttgt aaaacataag
    80281 gataagtaag agcagtagat ccatttatcg tggatatgct gctttttttg cggaaaataa
    80341 tcagcgaatg tttatttgat cggatgattt gtaactcgca gagtactaga tgcaagcgtg
    80401 tattcttttt ttgaacgata atcacaaaaa aaaata
//
LOCUS       DAJSBN010000006        72629 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJSBN010000006 DAJSBN010000000
VERSION     DAJSBN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.006"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..791)
                     /locus_tag="ONT86_000463"
     CDS             complement(<1..791)
                     /locus_tag="ONT86_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2351283.1"
                     /translation="MFPFFKKKPLKLQNTIILVVCFSTFVSLIVSAILIRNFILSMNR
                     SSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIMIASNLDFIVVMDPEGIR
                     YSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFRYFVPIYYDQEIIGAVCV
                     GLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMKKVLLGLEPQEIAEQLSE
                     KTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDFEVGK"
     gene            966..1490
                     /locus_tag="ONT86_000464"
     CDS             966..1490
                     /locus_tag="ONT86_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR2351284.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            1506..3005
                     /locus_tag="ONT86_000465"
     CDS             1506..3005
                     /locus_tag="ONT86_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR2351285.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3093..3695)
                     /locus_tag="ONT86_000466"
     CDS             complement(3093..3695)
                     /locus_tag="ONT86_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2351286.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            3799..4755
                     /locus_tag="ONT86_000467"
     CDS             3799..4755
                     /locus_tag="ONT86_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCR2351287.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(5060..6016)
                     /locus_tag="ONT86_000468"
     CDS             complement(5060..6016)
                     /locus_tag="ONT86_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2351288.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            6190..6750
                     /locus_tag="ONT86_000469"
     CDS             6190..6750
                     /locus_tag="ONT86_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351289.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            6743..7567
                     /locus_tag="ONT86_000470"
     CDS             6743..7567
                     /locus_tag="ONT86_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCR2351290.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            7577..8194
                     /locus_tag="ONT86_000471"
     CDS             7577..8194
                     /locus_tag="ONT86_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR2351291.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            8212..8895
                     /locus_tag="ONT86_000472"
     CDS             8212..8895
                     /locus_tag="ONT86_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR2351292.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            9578..10813
                     /locus_tag="ONT86_000473"
                     /pseudo
     CDS             9578..10813
                     /locus_tag="ONT86_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            10821..11708
                     /locus_tag="ONT86_000474"
     CDS             10821..11708
                     /locus_tag="ONT86_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2351293.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            11725..12561
                     /locus_tag="ONT86_000475"
     CDS             11725..12561
                     /locus_tag="ONT86_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2351294.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            12564..13187
                     /locus_tag="ONT86_000476"
     CDS             12564..13187
                     /locus_tag="ONT86_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351295.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            13184..14476
                     /locus_tag="ONT86_000477"
     CDS             13184..14476
                     /locus_tag="ONT86_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2351296.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      14835..14932
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15048..15629
                     /locus_tag="ONT86_000478"
     CDS             15048..15629
                     /locus_tag="ONT86_000478"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR2351297.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            15632..16939
                     /locus_tag="ONT86_000479"
     CDS             15632..16939
                     /locus_tag="ONT86_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCR2351298.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            17232..17720
                     /gene="purE"
                     /locus_tag="ONT86_000480"
     CDS             17232..17720
                     /gene="purE"
                     /locus_tag="ONT86_000480"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR2351299.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            17713..18837
                     /gene="purK"
                     /locus_tag="ONT86_000481"
     CDS             17713..18837
                     /gene="purK"
                     /locus_tag="ONT86_000481"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR2351300.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            18850..20145
                     /gene="purB"
                     /locus_tag="ONT86_000482"
     CDS             18850..20145
                     /gene="purB"
                     /locus_tag="ONT86_000482"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR2351301.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(20325..20702)
                     /locus_tag="ONT86_000483"
     CDS             complement(20325..20702)
                     /locus_tag="ONT86_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351302.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            20958..21686
                     /locus_tag="ONT86_000484"
     CDS             20958..21686
                     /locus_tag="ONT86_000484"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR2351303.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            21686..21940
                     /gene="purS"
                     /locus_tag="ONT86_000485"
     CDS             21686..21940
                     /gene="purS"
                     /locus_tag="ONT86_000485"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCR2351304.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            21942..22613
                     /gene="purQ"
                     /locus_tag="ONT86_000486"
     CDS             21942..22613
                     /gene="purQ"
                     /locus_tag="ONT86_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCR2351305.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            22614..24836
                     /gene="purL"
                     /locus_tag="ONT86_000487"
     CDS             22614..24836
                     /gene="purL"
                     /locus_tag="ONT86_000487"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCR2351306.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            24821..26260
                     /locus_tag="ONT86_000488"
     CDS             24821..26260
                     /locus_tag="ONT86_000488"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR2351307.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            26283..27335
                     /locus_tag="ONT86_000489"
     CDS             26283..27335
                     /locus_tag="ONT86_000489"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR2351308.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            27332..27910
                     /gene="purN"
                     /locus_tag="ONT86_000490"
     CDS             27332..27910
                     /gene="purN"
                     /locus_tag="ONT86_000490"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR2351309.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            27907..29442
                     /gene="purH"
                     /locus_tag="ONT86_000491"
     CDS             27907..29442
                     /gene="purH"
                     /locus_tag="ONT86_000491"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR2351310.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            29458..30732
                     /gene="purD"
                     /locus_tag="ONT86_000492"
     CDS             29458..30732
                     /gene="purD"
                     /locus_tag="ONT86_000492"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR2351311.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            30854..31768
                     /locus_tag="ONT86_000493"
     CDS             30854..31768
                     /locus_tag="ONT86_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2351312.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(31901..33466)
                     /locus_tag="ONT86_000494"
     CDS             complement(31901..33466)
                     /locus_tag="ONT86_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR2351313.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(33459..34910)
                     /locus_tag="ONT86_000495"
     CDS             complement(33459..34910)
                     /locus_tag="ONT86_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2351314.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(34924..35940)
                     /locus_tag="ONT86_000496"
     CDS             complement(34924..35940)
                     /locus_tag="ONT86_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2351315.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            36160..37248
                     /locus_tag="ONT86_000497"
     CDS             36160..37248
                     /locus_tag="ONT86_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCR2351316.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            37302..38828
                     /locus_tag="ONT86_000498"
     CDS             37302..38828
                     /locus_tag="ONT86_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR2351317.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            38830..40035
                     /locus_tag="ONT86_000499"
     CDS             38830..40035
                     /locus_tag="ONT86_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2351318.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            40292..41269
                     /gene="guaC"
                     /locus_tag="ONT86_000500"
     CDS             40292..41269
                     /gene="guaC"
                     /locus_tag="ONT86_000500"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR2351319.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            41533..42957
                     /locus_tag="ONT86_000501"
     CDS             41533..42957
                     /locus_tag="ONT86_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR2351320.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            43418..44527
                     /gene="pdhA"
                     /locus_tag="ONT86_000502"
     CDS             43418..44527
                     /gene="pdhA"
                     /locus_tag="ONT86_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCR2351321.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            44530..45507
                     /locus_tag="ONT86_000503"
     CDS             44530..45507
                     /locus_tag="ONT86_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCR2351322.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            45532..47175
                     /locus_tag="ONT86_000504"
     CDS             45532..47175
                     /locus_tag="ONT86_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR2351323.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            47183..48589
                     /gene="lpdA"
                     /locus_tag="ONT86_000505"
     CDS             47183..48589
                     /gene="lpdA"
                     /locus_tag="ONT86_000505"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR2351324.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            48751..49026
                     /locus_tag="ONT86_000506"
     CDS             48751..49026
                     /locus_tag="ONT86_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCR2351325.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            49032..49799
                     /locus_tag="ONT86_000507"
     CDS             49032..49799
                     /locus_tag="ONT86_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCR2351326.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            49967..51796
                     /gene="typA"
                     /locus_tag="ONT86_000508"
     CDS             49967..51796
                     /gene="typA"
                     /locus_tag="ONT86_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCR2351327.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            52057..53829
                     /gene="recQ"
                     /locus_tag="ONT86_000509"
     CDS             52057..53829
                     /gene="recQ"
                     /locus_tag="ONT86_000509"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCR2351328.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            53943..54245
                     /locus_tag="ONT86_000510"
     CDS             53943..54245
                     /locus_tag="ONT86_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCR2351329.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            54248..55411
                     /locus_tag="ONT86_000511"
     CDS             54248..55411
                     /locus_tag="ONT86_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR2351330.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            55437..58865
                     /locus_tag="ONT86_000512"
     CDS             55437..58865
                     /locus_tag="ONT86_000512"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCR2351331.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            58886..60010
                     /locus_tag="ONT86_000513"
     CDS             58886..60010
                     /locus_tag="ONT86_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351332.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            60000..60437
                     /locus_tag="ONT86_000514"
     CDS             60000..60437
                     /locus_tag="ONT86_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR2351333.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            60421..60759
                     /locus_tag="ONT86_000515"
     CDS             60421..60759
                     /locus_tag="ONT86_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCR2351334.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            60878..61438
                     /gene="rsmD"
                     /locus_tag="ONT86_000516"
     CDS             60878..61438
                     /gene="rsmD"
                     /locus_tag="ONT86_000516"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCR2351335.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            61435..61926
                     /gene="coaD"
                     /locus_tag="ONT86_000517"
     CDS             61435..61926
                     /gene="coaD"
                     /locus_tag="ONT86_000517"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR2351336.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            61916..62959
                     /locus_tag="ONT86_000518"
     CDS             61916..62959
                     /locus_tag="ONT86_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCR2351337.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            63118..63846
                     /locus_tag="ONT86_000519"
     CDS             63118..63846
                     /locus_tag="ONT86_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR2351338.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            63962..64207
                     /locus_tag="ONT86_000520"
     CDS             63962..64207
                     /locus_tag="ONT86_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR2351339.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            64204..64422
                     /locus_tag="ONT86_000521"
     CDS             64204..64422
                     /locus_tag="ONT86_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR2351340.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            64558..65097
                     /locus_tag="ONT86_000522"
     CDS             64558..65097
                     /locus_tag="ONT86_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCR2351341.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            65090..67363
                     /locus_tag="ONT86_000523"
     CDS             65090..67363
                     /locus_tag="ONT86_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCR2351342.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            67401..68447
                     /gene="holA"
                     /locus_tag="ONT86_000524"
     CDS             67401..68447
                     /gene="holA"
                     /locus_tag="ONT86_000524"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR2351343.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(68536..68790)
                     /locus_tag="ONT86_000525"
     CDS             complement(68536..68790)
                     /locus_tag="ONT86_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR2351344.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(69190..69459)
                     /locus_tag="ONT86_000526"
     CDS             complement(69190..69459)
                     /locus_tag="ONT86_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351345.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(69929..70384)
                     /locus_tag="ONT86_000527"
     CDS             complement(69929..70384)
                     /locus_tag="ONT86_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR2351346.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(70522..72450)
                     /locus_tag="ONT86_000528"
     CDS             complement(70522..72450)
                     /locus_tag="ONT86_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR2351347.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        23651 a        12522 c        15835 g        20621 t
ORIGIN      
        1 ttttttccta cttcgaaatc caatagctgc tctgtttggt agaaaatgtt ttttgcaggt
       61 gtattgatca agaggatagt tttatcagaa gagatggcta agattccttc atctacttca
      121 ttttcgataa tcgttttttc acttaactgt tctgctattt cttgtggttc aagtccaagc
      181 agtacttttt tcattttttg ggcaagaaaa acggcgccta tgatgcctac gctaagtcca
      241 ataatcaatg aaaaaacaat cattttttgt ccatctttga cttgattgtc aatcgtgttg
      301 tttgttaagc caacacaaac tgcaccaata atctcttgat cataataaat agggacgaaa
      361 tagcggaagc cgaaccctaa cattccttct ttttcagaga aaaattcttg tcctccaatc
      421 acttttttca catcttcatg acttgagaaa ggaagaccga tgtaacttgg gtctgcgtga
      481 gaatatcgaa tgccttcagg gtccatgaca acgataaaat ctaaattcga tgcaatcatg
      541 atttcatttg ttattccttg aatcgcatgt gtcgggtcac cagtctgcag ctttttttgg
      601 atgagtttat cattggcaac gattttagca atgctagaga ttttatctga cacactagat
      661 cggttcatcg aaagaataaa attcctgatc aatatggcag aaacaatcaa agaaacgaat
      721 gtggagaaac aaacgacaag gatgatcgtg ttctggagct ttaatggttt tttcttaaaa
      781 aaaggaaaca taggctacct cgaaagaatt tattgtgaaa attttaacat gaaaacaaaa
      841 atttaacaaa ttgtcaacga taaacacaac cttcctataa aaaacaccac tgagtcggta
      901 aaagaagcta tacagaattt tcaaacattg gtatgataga cgtagaaatg gaaggaggcg
      961 tgtatatgat agggggaatc gcacttgaga cgatttattg gtatacattg ctgatttgtg
     1021 caggattggc agtcctattg attatttttg gagatgtatt cgattttgat ggcccgcttg
     1081 atccgatgct tattatccct tggttaactt tcacaagctt atttggtttt ctaggtgagc
     1141 gtttaacaga tcaatcatct ttatttttat ttattgctag tggaggcata tcgatagtgt
     1201 tggtattttt gcttaatttc tatatattga tgccgatgaa acatgccgaa tcaacgatgt
     1261 cgtcttctga aaaaactctg gaaggacaag tagctgttgt gatcacaccg atccctgtac
     1321 gaggtatggg agaaattcag ttgaaaagtg ttaccggttc cattagtcgt ccagcttgtt
     1381 tttacagccc ccaagaacag acagctgagc ggggaacgaa agtattgatc attgagatca
     1441 aggaaagaat ctgttatgtc atgccttatg aaagcatgat aaaagcttaa aggagcgtgt
     1501 gttaaatgga aataccagtt gtttttatcg cgatcgcaat cgcagctttg attttactga
     1561 tgttgctgat cgtctttgta gcgaagtacc agacagcaaa accggatgaa gcgttgatca
     1621 tcagtggaag ttatttagga agcaaaaatg tgcacattga tgaaagcaat aacaaaatca
     1681 aaattgtgcg tggaggtggc gcattcgtct tgccagtttt tcaacgttcg aatcggatca
     1741 gtctattatc gagtaaattg gacgtctcca cccctgaagt gtataccgaa caaggcgtac
     1801 cggtgatgtg tgatggtact tcgatcatca aaatcggttc atctgtagaa gagattgcta
     1861 cagcagcaga acagttttta ggcaagacaa gagaagaact ggaaaatgaa gcaagagaag
     1921 tattagaagg acacttgcgc tcgattcttg gttctatgac tgtagaagaa atctatcaaa
     1981 atcgcgataa gttcagtcaa agcgtacaag aagtcgcttc tgtagacctt gcaaaaatgg
     2041 ggttgatcat cgtttctttc actatcaaag aggtcagaga caaaaacggt tatcttgact
     2101 ctttagggaa accaagaatc gcccaagtca aaagagatgc agatatagca gaagcggaag
     2161 ctctaaaaga aacgcgtatc aaaaaagctc aagcagaaaa agaatcacaa gcagctgaat
     2221 tacagcgaca aacagagatc gcagaatcat taaaagaaaa agaactgaaa ttagcaacct
     2281 acaaacaaga acaagatgtt gctaaagcaa aagcagacca agcctacaat ttagaaagtg
     2341 cccgagcaca acaacaagta atcgaacagg aaatgcagat taaaatcgta gaacgccaaa
     2401 aacaaatcga actggaagaa aaagaaatca cacgtcgtga aaagcaatat gattcggaag
     2461 tgaagaaaaa agcagatgcc gatcggtacg ctaaagaaca agaagcacaa gctcaaaaag
     2521 tcaaagaagt gactgaagcc gaagcagaac gattccgtgt ggaagcactg gcagaagcgg
     2581 aagccaataa gacacgttta gctggacaag cagaagccga agcagcacta gcaaaaggaa
     2641 aagcagaagc cgaagcaaaa caaaagatcg ccaatgcctt caaggaatat ggcgaagcag
     2701 ctgtcttgag tatggttatc gatatgctac cgcaattgat gcgcgaagca gcacagccgc
     2761 taggaaatat cgaaaaaatc tctgttgtcg atacaggaag cagttcagga gaaaccagtg
     2821 gagcgaaccg agtgacaaat tatgccacaa atttgctttc ttctactcaa gaaacactaa
     2881 aagcgacaac aggacttgac ttgaaggaaa tcgtagagaa tttgtcgaca cgtgggacta
     2941 ctagccaagt aaatatgtat gaagcgccaa aagaggcaga acaaaactcg atgaaggaag
     3001 aataaagtag tacacttcac cgttcaatca aaaatggagg aagacttttt aaagttcttc
     3061 tctccatttt tttattttat tgcaaaagaa aattagtcct cgctattttt cttttgtttt
     3121 tggatcttag cttctatctc ttgtaaattt gttcttagca atcgttcttt cttttggagt
     3181 tcgaccaaat cttctgatag ttcttccttc ttttctgttt caggaggacg aagaaagaaa
     3241 ttgataccga ttgcaatgaa tgtccctata aaagtatcta gtacacgttg gatagcatac
     3301 aaagcggatt cgccttgagg gatgctcaac gcaatcagca acaacgtagc tattgcggaa
     3361 atgataccgg aattattgtt aataccatct gacacgacga tcacaataat aactagaata
     3421 ggaagcaaga tcaattctac caaaaaatca ttgtgaaaat agcttttgac aaaaaaataa
     3481 atgatagcta atgtaccacc taatgtattg ccgatgatcc tagactttcc aaaagaaacg
     3541 gttgtagtca agtcctgacg caaagaaaaa acagcagata atgcagcaat catcgggatt
     3601 cctcgttgag tcaaatgaaa aaacaagata cataaaaaaa cagctaaggc acttttgatc
     3661 gtacgcatac ctagtcgaaa acggccaaat tgcatagaga ttctcctttt tggtcttttt
     3721 actatcatac ctaaactttt gtctgatagc tagtttttca ttattcaagt ataattaaaa
     3781 taaaaaaggg ggaagtaaat gcgtatagga atgcgaacag tcaaaacagt gatcagtgtc
     3841 ttcttttcga tggtcacagc gggtagctta tcgctgttgt attggccagc tgcagggatc
     3901 attgcattat taagtgtagg aaataccaag cgctctacat tgcgtacggg catgaatcga
     3961 atcgcagcct ttatattagc tacggcaaca gcactgttca gtttttttgc tgttgggtat
     4021 actatttttg gcttcagtct ttttttattc ctgttcatcc cgcttgcctc tcgcttcaaa
     4081 ttaaccgaag ggatagtagt aaattcggtt ttggtcactc attatttagt ggaggaaaac
     4141 atgtcttggc agctgatcgg caatgaagcg ggattaatgg cgattggatt agtatttgct
     4201 ttgcttgcaa atgtttacat gccagatcgt acgaagcagc tgaaggaaaa ccaaatacag
     4261 atcgaaaaag aatttcgaaa tattttgaga caaatggcag aatttctttt atcagaaaat
     4321 aaagaagatg tgcagatcaa atgcgaacat ttactgacat tcattcgcga aagccaagaa
     4381 gatgccagag aacatcaaga aaactactgg ctcagacagc cgctatacta cgaaacttac
     4441 ttctctatga gaagagcaca ggccaatgtg attaaagaca tgctggaaaa tctagagaga
     4501 atccagcaac cagcttatta tggaaaacat atctatggtt tattgattta tactgcagaa
     4561 acattttcag agtcaaatga tggcagacaa attttaacac gtattgaaga agtttatgta
     4621 ttgtatcggc aaatgcctct gccgactagt cgccctgaat tcgaggatcg agcagaattg
     4681 ttccagtttt tgcagtcttt taaaagtttt attgaaatca aggcagaatt ctcccaacaa
     4741 aaaatccaaa aatagaggtt gtgaattcag ctaatattca ggtcaaaaag ctgatttcac
     4801 aacctcttct ttaaacggtg ttctcctgtc aactcctcgg tcacaagtca caatattcaa
     4861 caaacatgag aagctgtttt ttgaaattgc tccttgtgac tcggagctga acggtaggct
     4921 cacaacctct ttcttaaacg gtgttcaaaa gctttcctct gcggtaataa gtccagccaa
     4981 acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct
     5041 ttttcacaac cttttaaaat tacatatttt tccatagaac ttgcatttga tccaagattt
     5101 cgatgacatc ttttgtcaag aagagagagc gattgggcaa gtcaccttca atcatgtgta
     5161 ccatattctt tatttcgtaa ttcattgctt cagatgtatt cccgctttcg atgaattcct
     5221 ttgtaccatc tgtaaataag atctccgcac gatcagcacg cgggtaatca gcaatcgtca
     5281 cataaccgtt ctcaaaggca acgataccga cttttggcat ttttgcctga aaagtcaagc
     5341 tgacagcagc aagttcattt tctttgttac gcaagatagt gacggattgt tcgtccacgc
     5401 ctgtttcaaa aggaaccatt gtagaggcaa ctacttcagg ttgagagctc aaaaagaaac
     5461 gagcgaaaga tacagcatac gtaccgatat ccaacagagc tcctccagct aattctggat
     5521 tgaagaaccg gtttttaggg tctggttctt tgtaactgcc gaaaggtgct tggatcattt
     5581 ttaaagcccc cagttttcct gtatccatta ttgagcgtaa ctgctgataa agtggcatgt
     5641 tgaagattgt catcgcttct gctaagatca aattcttttc ttctgctaag cgcatagcgt
     5701 cagccagttc ttttttattc atcgtgatgg ctttttcaca tagaacgtgt ttgcctgctt
     5761 ccaatgctgc caagatatgg tcgatatgct gtctatttgg tacagcaatg tacacgatgt
     5821 cgatttcagc atcgtctaac atttcttgat agcttccgta agcttttgga atattgtaac
     5881 ggtgagcgaa gttttccgct ttttctgaag ttctggaagc aactgctgtt agttcgctcg
     5941 tctcttgatt gaagctttcg gcaaactcat gtgcgatggt accaagacca acgattcccc
     6001 attgatattt tttcatctga aaacgctcct tcatagttgg attgagtttc ttgtctgtct
     6061 attattataa caaaaatcac gaagaataaa gaggagagga cttgaaacat taaaaaaaat
     6121 gaaagcagcg atttaagagg tgctttttga tcaaaactta agtagaatag ttaaaaggag
     6181 gggataaacg tgcatcaagt atatattgat cttattatga gtgtattatt agaacagttt
     6241 gaaactgaaa aaaatttttt agatgaatac ttgctgatca gtccagaaca atggcaaaaa
     6301 tggaaagatg gcgagaaaaa tctgccatct gaagcgatgc agaaagtaaa aaatcttttc
     6361 actgattatg aatggatgct aacgcaaaag attttgcgtc aaacgattat ttttcccgaa
     6421 aaacgaaacg ttgccgttgc agagtacaag caaatcaaaa cacgcatcgc gcaaaaatgg
     6481 cttagcagca aagcagcaaa agtcgagctg atccctttga aaaataatca gcaggctggt
     6541 agataccttg acttgaaagt aagtattcaa tatgatgaat ggggctatga tgatattttg
     6601 aattttcgtc tgccagcaga tgttcaaaaa cagatcgaag gagaaaaaat cgaacttctc
     6661 gattgggtga atgaaaatct ggaagaaact tacgttcatg aggcgaatgg ggaacgtgaa
     6721 gaacaacgaa aggatttttc aagtgaataa atttttagga tttggagcag ttgtgattat
     6781 gatcgcagca atgggctatg ttatttttag cgatgatgat tcgcatgttc aagccggcaa
     6841 tcaagcagtc gaacgaacaa gcaccaaaac aactgctagt aaagaaaaga acagtcaaac
     6901 aaccagcagc aaggtaaagg acaaagaaac aaaagatcgt acgtccaaac aattgccaga
     6961 tgttaaaaca gacgattgga agctagtttt ggttggtcca gatcacaaat tagaaaaaga
     7021 aattgatgag tctactcaat tggcatcatt aagtaacgga tacttgatcg acaaacgcat
     7081 tgctccagaa tatgaagcat ttgccaaggc tgctgaagca gctggatttc cattagtgat
     7141 ggtctcagct taccggtctg tatcatccca acaacaagtt ttcgaacaaa atgtccaaga
     7201 tgtgatgagt cgtcaaggtg tatcagaaga ggaggcgaca aagattacta aagaaacgat
     7261 tacggagcca ggatacagcg aacaccacac aggtctggca gtcgatgtcg tcgatcaaaa
     7321 ctggtacaac agttatcctt ctactgtatt ggatgcaagt tacggagatc agcctggagc
     7381 taaatggatc gcagataatg ctgcgcagta tggatttatc gttcgttatc caaaaggaca
     7441 cgaagatata actaagatca catatgaacc ttggcactta cgttatgtcg gtaaagaaaa
     7501 cgctgagtat atcaccaaac accaattgac gctagaagaa tatctgaatg aattaaatga
     7561 aaagtaggaa ttgatcatgg cgaaaaaaaa gtggaaaaag caaaagcgca agaatatccg
     7621 ttggccagca gtggttgccg gtgtagcagt gatactctta gcctttgttt tttccttgaa
     7681 gaatctgtct tctcctttta ctgataatca gcaagaaaat gaagcgcaat cccatcagca
     7741 atttattgaa cggcttgttc cgcatgccca agaactacaa gatggttatg gagtgcttcc
     7801 tagtatcatt ttaggacaag ctatattaga atcaaattgg ggaaaaagta ccttggctag
     7861 caaatacaat aatcttttcg ggatcaaggc ttatggggat cagaagaaag taagcctgga
     7921 aacaaaggaa tttgttaacg aagaatggat aactatccaa ggagacttca aagtgtatga
     7981 ttcatgggaa cagtccatgg atgaccatac acaactgttc gtccaaggag tagattggaa
     8041 tcctgcgctg tatgaaaaag tgattacggc caccaactat caggaagccg cacaagcatt
     8101 gcaagatgca ggttatgcga cagaccccgg ttatgcgcaa aaaatcatcc aggtcatcga
     8161 gacgtatcaa ttgaatcagt atgaccggag atagcaaagg agaagtggct gatggcagaa
     8221 caatttacgc cagaaatcat gacggaattg agaaaagata tcgtaagagt accggaaatc
     8281 attttagaag caagcgggat caagatattt ggaaaattga tccgttcgat catctttact
     8341 acggatattg cgatcatacg taatacaaat gcgaatgcag taattgctgt gtatcctttt
     8401 acacctcatc cagcgattac taaaagcatt attgaggcag ctgacattcc agttttctcc
     8461 ggtgtaggtg gtggtttgac acaaggtgct cgttcttcct acatgagcat gttcgcagaa
     8521 gcgcaaggtt cgttaggggt cgttttgaac ggtcctacgc ctttagagac agtaaaagaa
     8581 gtttgtcgag tagttgatat tccagttatc agtacggtaa cgtcgaaata tacagaaata
     8641 gatgaaaagt tagcagctgg tatttcgatt atcaatatca gtgcaggtaa agaaactgca
     8701 gaagcagtac gccattttcg taaaaaatat cctgaacttc ctatcattgc gacaggtggt
     8761 ccaacggaag aaagtattgc tgagaccatt caagcaggag caaatgccat cacatatacg
     8821 ccaccaagta gcggagagct ttttagtcgt aaaatggaaa aatatcggga aactgagaaa
     8881 aagaatcatg aataacaggt gagaatcaaa agacgaggaa gagcagatag tggctcttcc
     8941 tcgtcttttg tactccagta ctatttgttc agaattcaaa aaaaagagaa atttttttgt
     9001 ttttttacct gtaatgtaca taagaagagt taaaaccttt tgttattatt agcattctaa
     9061 caaaaaaatg tcttgattta ttgaagcgct ttctatagaa tgaaaaacga ataaaaacta
     9121 aaaaaggcac ccgacaattt agtagcagct aaattgtcgg ggcttgaaaa gtcagcggga
     9181 actgaccaat caagttgcca tagtcaatgc ttgtgcatcg acgtgtatat tgtacctaat
     9241 ttcatacaga attactagtt atttttctgt tttttaggga agagcgcaca cgaagaagca
     9301 tgcttattta gctatgtcaa ggatattggg cagttgtctg atatcctttt ttctttgcgt
     9361 aattatcctc ccatcattga agcaagaagt gtttttagcg atgaaagaag aatcagcact
     9421 atctaatttt tcttgaaagt attgagttgt tcatcctagg cagaacgata aggcaagtct
     9481 tggtttttat tccccttgaa aaagatcgtt taataaatat gttctgtata aaaatacagt
     9541 atattgaaaa ataacaatgg aagtagaggt gcagagaatg aagaaaagaa tgagaatcat
     9601 cttagcttta agtgttatgt tattcttctt gacggcttgt gggcaagaag atgacaaaaa
     9661 aatcacgctg aacatggctt ggtggggttc gcaagtccga cacgatgcaa cagtaaaagt
     9721 aatcgagctt tatgaagaaa aaaatcccca tgtgacgatc gattatgaat tttatgatta
     9781 tgaaggatat tacacaaaat taggtacact agcagcatca gatgatatct gggacatttt
     9841 ccaactgaac aatcaatttc ctcaatacat caatcagatt gaaccattag atgaatatgt
     9901 ggagcaaggg ataattgata tttctaatgc agatgaatcc tatttagcaa cgacgactta
     9961 cgacgggcaa ttagttggac tgtctaacgg ggtgaattcg tttgcgattg cttacaataa
    10021 aaatatattt gaacaattaa atatccctga accagcttca aattggacat gggatgaatt
    10081 tgaacaaatc agctatcaaa tcactgaaga attaggaatc tatgctattt caagattcga
    10141 agattttata gctggctgta ttattcaaat accacaagtt gagaaaggat tgaattttta
    10201 taatcgggaa gatctatcta acagcttggg atttgaaaat ccagactatc taacagattt
    10261 tttccagctg cgtaaaaatc tggtagacaa aggggcccat ccagaaccag gtggtggagc
    10321 tgcaagtacg acggatctgt cttttcaggc ggttagggat agagaagcgg caatggtgtt
    10381 tctatcaagt aaccagttga cagaaatatt gtcaggggct cctgaagata tggagattca
    10441 gttgattaat ccacctcgca gaaaagcaga tggagaatct ggtatccctt tacgttcttc
    10501 gcaaatgctg agtatggcaa aaagctcagc ccataaagaa gaagctgcta agtttattga
    10561 tttctttcaa aacagtgaag aagccaatga aattttaaaa ggagaacgtg gtgtaccaat
    10621 catgtcgaat attcgagaac agctcgctcg tgcagatgat gcaactttgc aagcgacatt
    10681 tgattattta gaaatgattt ccgatatgga caacggcgaa gtgaatgtct ttgatagtgc
    10741 tttgaatccg gaaattcaag accaatacaa tcttttagtt gaaaaagtca tttatgaaga
    10801 agtaacagca gaaggaaggc atgaaaaaag aaaaactaaa acggttcttg gatcaagaca
    10861 atgtggtcgg ttacgtattt gcttctccat tcatcctagg atttctattg ataacagtga
    10921 taccgatgat cttgtcactt tattattcgt tcaccgatta tcgtttggga aatccagtct
    10981 catggatcgg cttggataat tatataaaga tgctgagtga tgcccgtttt ttgaactcca
    11041 tccgagtcac ttttctctat gtatttgcat ctgtacctgt gaaactgatc tttggtctat
    11101 tgattgctta tcttttaacg aggaatatca aaggaacgac tttctttcgt tctttgtatt
    11161 atgtgcctag tttgatcggt ggcagtattt ctatcgctct agtttggcgg gagttgtttt
    11221 ctaaaaacgg gttagtgaat attttcctgg aacgattagg cgcacctgcg ctttcctggt
    11281 ttagcgatca aaaactggtc atgatccctc ttgttttgat gtctgcttgg caattcggtt
    11341 cggcaatgat catctttgct gcaggtatca aagagatccc aggctcgtat tatgaagcgg
    11401 cagagatcga tggagcgaac aaatggcaga cgttttacaa gatcacgtta cctcttcttt
    11461 cgccgatcat tttgtataac ttggttatgc agacaatcgc ggcgtttatg acttttacgc
    11521 aggcatttat cattacccaa ggtggtccga atgatgccac gaacttttat gctctttatg
    11581 tgtataacca agctttttct tttaattcta tggggtatgc tagtgctatt tcgtggatca
    11641 tgctgttgat tatgggagct gtcacctttc tgattttcaa atcatcaaaa aaatgggtcg
    11701 ttgtgtgaat gagaggagga agaaatgaat aatacagtaa cgaaatggtt tcaatacggt
    11761 tttattttgc tattaggtct agtcatgatt tatccggtct tatggatgat cgcaggttct
    11821 tttaaaacgg atcaagagat tatgagtggt tcgctgaatt tgattccaga gacttttcaa
    11881 tgggaaaatt acgcaaaagg atgggcgggg tttgctggaa tttcattttt tactttcttt
    11941 aggaattcgt ttgtcatctc tgcagtttcg acgataggga cagttctgag ctcgacttgt
    12001 attgcttacg cattatcgag aatcgatttc aaaggaaaaa gattctggtt cgtcgtcatg
    12061 ttagtaacga tgatgattcc tgcacaagtg attttgattc cgcagtttat tatttacaat
    12121 cggctgaatt tggttgggac gtatgtgccg ctgattttac cacatttttt tggacaagca
    12181 tttttcattt accagatcat gcaattcatg gtcagcatcc ccaaagagtt agatgaatca
    12241 gcgattattg acggatgcag caagtatagt gtctttacca aaattatttt tcctttgctg
    12301 aaaccttcga tcatcacgac gatcatcatc cagttttatt ggaaatggga cgacttcatg
    12361 ggaccattga tttatttaaa caagccgaga tcttacacgg tatcgattgc catcaaatta
    12421 tttgcagacg ctggatctag tacgacttac gcgagtatgt ttgcgatgtc tacgttgtcg
    12481 ctgctaccgg tttttctgat tttcttattc ttcaataaat atcttgtcca ggggatcagt
    12541 acaagcggtc tgaagggctg aatatgaaaa agccaataga agagatgttt ctttacaagc
    12601 tcaactacta tgtctgtcgg atttttttaa ccagtcttat cagtaatgct cttttcgctt
    12661 tagttctagg atgtttgttc actttaggat tagtgctgtc tgctcgtgtt gtctatctga
    12721 tttatttttt gatgatgtat ccttcttatt atctatctgt ccattacttg gcagaaaaaa
    12781 aggtatgttg gaggaaaggg aaaatcaaac ggatgtgttt catcattacg ttgtttcagc
    12841 tgtttgtcat tatttttgca gttgattggt atttattagt gggaaagatt ggagcttggt
    12901 atctgattcc ttttctattg tgtctgttgc tttttgtatc ccatagtttt tttagtacgc
    12961 taattgatat ctgcaaagaa gataaagcag atagtcttgt ttatttgatc aaaaggaata
    13021 tctggctgtt gtcaaaaaac gtcaaggggt ttctgtggta tttatgcctt ttgtatggac
    13081 tgttctggtg tatacagcct gattttttac tcactgcatt actgtatcca gggctgccat
    13141 ttgtcacagt attttgctta aaagaaaagg agaatgactt taaatgaaga aaaaatatgt
    13201 acaagtcgga cttggtggga gagcgcgttt tttttatgag gcaattgcct ctacgtatca
    13261 tcaaacttgt gaactagtcg gtttttgtga tgtatctcaa acaaggatgg attatgcaaa
    13321 tcggatactt gtgaacaagt atggacagaa aaaagtaaag acatgtatgc catcacaatt
    13381 tgagcaacta ctggatgacg tacggccggc tgaagtcatt attacgtcag tagatcgaac
    13441 gcatcatgat tatatcattc gtgcaatgaa aaaaggatat gatgttattt gcgaaaaacc
    13501 tctgactatc gatgaggaaa aagctcaaga aataataacg gtccaaaaac agacgggaag
    13561 aagcctgcga gtcactttta attatcgtta tgcaccgtat cacacgaaga taaaggaact
    13621 gatagaaagt gatgtgattg gtgatgtgtt ttctatccac ttcgaatggc tgttaaatac
    13681 gcaacacggg gcagattatt tcagaagatg gcatcgaaat aagcacaata gcggcgggct
    13741 tcttgttcat aaatctaccc atcattttga tctagtgaat ttttggttga acagccgtcc
    13801 ccaaaaggta tttgcattcg gtggattaaa cttttatggg atgcataatg cggagaaacg
    13861 cggagtagaa atattttaca gtcgatgcta tggaagcgaa ctagctaaag ctgatccatt
    13921 tgctatagac ctgtcagcca atgaggaatt gcgagaaatg tatttagaag cagaaaaaga
    13981 cagtggatat attcgtgacc gcagtgtatt ttccgatgat atctctattg aagatacact
    14041 aggcgtaacg gtcaaatatg aaactggtgc gattttgacg tattcgctaa acgcgtatat
    14101 gccgtgggaa ggattcaatg tttctttcaa tggcagcaaa ggacggatcg aaatgaaagt
    14161 gatcgaaaag tcttacatca atgcaggtgg agataaaaag gatgaaggag cagccgttct
    14221 tcagtccatc catgtctatc cgatgttcgg tgagccatat gaggtaatcg tcgaacaaga
    14281 agcgggcgga catggcggag gagatccgcg tttattagat gatctatttg gtgaagaaaa
    14341 agaagaagat ccgtggaatc gggcagcaac tcacgtagat ggtattttgt cgattttgac
    14401 aggaatcgca gcaaatcatt ctattgcttc aggaaaagca gtagtgattg acgagcttgt
    14461 cagcttaaaa gaataagaat agatttacgg aaattacttc ccgtagttta taaaatcttg
    14521 ccttattgcc gataggctgt cttttgaaca ctatttattc ggaaaaaggg gctatgacat
    14581 gctttttgtc atgccttctt tttatgtggg ctgctttttt agataggaga aaataaaaat
    14641 aactatttaa acccgaacaa aaaggaataa tgcattaaaa atcattagat tttacaaaaa
    14701 aataagtaaa ccacgttgtc agtttcagaa agtagtgtta gactgttttc aatatttgaa
    14761 acaacatttt ttattcaaat atggcaaact gccttcacgt gcaaaacagt tgcaagaaaa
    14821 tgaatttaca ctattattag ttttacatac ctacttatat atcgtcataa tatggatgac
    14881 agtttctagc cagtaccgta aatgctggac tataagtaaa agattggcta tttcaccctt
    14941 cttttcttat gataaagtgg agggtgtttt tctttaacag aaacatagta ggtttttgca
    15001 caaaagcggt tatttcggta ttaataaatg ataaaggagc aaataaagtg aaagaattag
    15061 ttgaacgtat tcaaaaagac ggacgtatat taggcgaggg cgtattaaaa gtcgacagct
    15121 tcgtcactca ccaagtagac cctgtattga tggaacaaat gggaaaacgg tttgcagaag
    15181 tatttaaaga acaaggaatc acgaaagttg tgaccattga agcttctggg atcgcaccag
    15241 ctttatttgc tgcaagagaa ttagatgttc caatgatctt cgctagaaaa gcaaaaagtt
    15301 tgacgatgga tgaagaatta ttgacagctt ctgtttattc ttttacgaaa caagtgacaa
    15361 gtacgatttc tatttctcgt aaatttttat cttcagaaga caaagtattg attatcgacg
    15421 atttcttggc aaacggacaa gcggcaaaag gattgatcga attatgccag caagctggag
    15481 cgcaagtaga aggaatcggg atcgtcatcg aaaagtcttt ccaagatgga cgtcagttac
    15541 ttgaagaaat gggattgaga gtcgtttctc ttgcgcgtat tgcttccttg acgaatggac
    15601 aagttgagtt tcttgaggag gacgcataat cgtggaaaaa gaaacacaaa atggaaaagc
    15661 tgcggtttta gggctgcagc atctgctagc gatgtatgca ggagcagtag cggtgcctct
    15721 tttgatcggt acaggactag gctttaatca agaacagatg acttatttga tttcaattga
    15781 tatctttatg tgtggattag cgacattatt gcaattgaca gttaaccgat ttttcggaat
    15841 tggcttgcct gtcgttttag gatgtgcgat ccaagcagta gctccactga tcttgatcgg
    15901 aaccaatgaa ggagtaggag ctatctacgg atctattatc gcttctggga tctttgttgt
    15961 gttagtttct ggtgtctttt ctaaaattaa aaaactattc cctcctatag taacaggaac
    16021 ggttatcacc gttattggtt tgactttgat tcctgtagca atcgagaaaa tgggtggagg
    16081 taatgcaaca gcagctgatt ttggtgacaa aacaaatctg ctacttgcat ttgtgacgat
    16141 tctcctaatt gtaggcgtgc aaatgctggc aaaagggttc attcgttcaa tcgcggtatt
    16201 gattggttta gtcggaggaa gcatcttagc tgctttcatg ggaatgattg atctgagcgc
    16261 gattggagat gcgccgttgt tccatgttcc acaacccttt tattttggta aaccaacttt
    16321 cgacgtatgg tcgattttac taatgatcat catttccatg gtcagcatgg tagaatcaac
    16381 aggtgtatat tttgcacttg gtgacattac tgggaaaaat gttggagaag aagaattgaa
    16441 aaagggatat cgtgcagaag gacttgccgt tatcttaggc ggtattttca atacattccc
    16501 ttatactggc ttttctcaaa atgtcggatt ggttcaactt tcgggaatca aaacaagaaa
    16561 acccatctat ttctctgcat tcttcttgat tgttttagga ttattcccta aaatcggtgc
    16621 gttagcacaa atcatccctg agccggtact tggtggagga atgttagtca tgttcgggat
    16681 ggtggcagtc caaggaatcc gtatgctgtt ggaagttgat ttcagcaatg ataaaaatct
    16741 attgattgtt gctgtttctg ttggatttgg gctgggtttc aatgttacgc cgacattatt
    16801 cagtcaaatg ccgcaaacat tgcaaatgtt cactggaaat ggcatcgtaa tgagtagcat
    16861 tacggctatc atattgaatt tgatctttaa tggattgaaa aaagatgaag aaatattgga
    16921 agaagccttc aagaagtaat agagcaaaag aaaggttgtg aaatcagcgt tttgacctga
    16981 ttgttagaga aacaaacgga aaaatagttc ctcgtatttt gccgtttgtt tgagctaata
    17041 ccgagggtca gctgattgaa caccgtttat ccataaaaag gaatgtgacc taacttttgt
    17101 cacattcctt tttttcatct ttaataaaca cgaacaataa aatgtttttc tttgttaaca
    17161 ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa tatgaatgta gaaaaactgg
    17221 gaggaaaaac aatgacagca aaaatctctg tgattatggg gagtacttca gattgggaaa
    17281 caatgaaaga agcatgtcaa atattggatg aattcggtgt gtcttatgaa aaaaaggtcg
    17341 tttctgctca tcgaacccca gatttgatgt ttgaatatgc acaaaacgct cgaaaaaaag
    17401 gaatcaaaat cattattgca ggagcagggg gagccgcgca tctgccgggt atggtcgcat
    17461 caaaaacaac tttgccggtt atcggtgtac cggtccaatc acgaacatta aatggtttgg
    17521 attcgcttct ttcgattgtc caaatgcctg gaggagtacc tgtagctact acagcaatcg
    17581 gaaaagcggg agcaattaat gctggactac ttgcgataca gatgctttcc atgtatgatt
    17641 tagaattgga ggctaaattg gctgaaagac gttcaatgtt ggctaaaact gttatagaaa
    17701 gtagtgatca acttggttaa acctttgttg cctggttcaa cgatcgggat cgtcggtggc
    17761 ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa tgggctttaa agtaggtgtg
    17821 ctggatcctg tgagcgattg tccagccgca caagttgcag attggcacat tgaaagtgtc
    17881 tatgatgaca cctttgcgct gaaagagctt gccagaagaa cagatgtcat tacctatgaa
    17941 ttcgaaaacg tcagtgtaga ggcgttaaat gcgattttac ccatgtcatt tattcctcaa
    18001 gggacagact tgcttgcgat tacgcaagac cgcctgttag aaaaatcttt tttggaaacg
    18061 aataacatcg tgattgctcc ttatgcaacg atcgtcagtc cgacggatat ccaagatgcg
    18121 attgacggga tcggctatcc ttgtgttttg aaaaccactc gaggcggcta tgatggcaag
    18181 gggcagtatg ttttgaaaag tcgggcggat ctagctcctg ccatggacct tttaagagaa
    18241 ggaacctgtg ttttagaagc atggatcccg tttgaaaaag agatttcgat catggtggca
    18301 ggaaatggtc aaggagattt cacgactttt ccggtcgtgg aaaacatcca ccacaacaat
    18361 attcttcatg aaacgattgc tccagccgcg attgatcagg atgtgatcga ggaggctgag
    18421 cgtattgccc gtgtgattgc agaagcagtg agcctttcag gtgttttagg ggtagaaatg
    18481 tttttaacaa aaacaggcgg gctatatgtg aatgaattag ctcctcgtcc tcacaattct
    18541 ggacattatt ctattgaagc ttgttctatg agtcagttcg atgcccatat tcgaggtatt
    18601 tgtggttggc cattaggaga aaaagtacgt ttgataagca atgcagtaat ggtcaatgtt
    18661 ttgggagaac aactgctgga aagctatcgc ttgattgaga ataagccaga ctggcaattc
    18721 catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga tgggacatat cactatcttg
    18781 acagaagaca tccagcaaac gctggaagaa atcaaacaaa cgaatatttg ggattagagg
    18841 agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg ggaatatctg gacggaagag
    18901 aacaaatacc gtgcgtggct cgaagtcgag attttagcag atgaagcttg ggcagaactt
    18961 ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc atgcatcatt tgatatcgat
    19021 cggatcttgg aaatcgaaaa attaacacgc catgatgtcg ttgcgtttac acgagcggtt
    19081 tctgaaacat tgggagaaga aagaaaatgg gtccattatg gtctaacaag tacggatgtc
    19141 gtagatactg cttatggcta tctcatcaag caagcaaatg atattttacg ggaagacttg
    19201 aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac acaaatatac agtgatgatg
    19261 ggtcggacac acggtgtcca tgctgaacca actacttttg gattgaaact tgccacatgg
    19321 tattcagaaa tgaaacggaa catcgaacga tttgaacatg cggcaaaagg agtagaagca
    19381 ggaaaaatct caggagcagt gggaacgttc gcaaatatcc cgccatttat tgaagagtat
    19441 gtatgtaaac aattggggat ccgtccacaa gaaatctcta ctcaagttct ccctcgggat
    19501 ctacatgctg aatatttctc agcgatggcg cttattgcta caagtattga acgttttgca
    19561 acagagatac gcggattgca aaaatccgaa acacgtgaag tagaagaatt ttttgcgaaa
    19621 ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc cgatcggttc agaaaatatg
    19681 gctggtctag ccagagtggt gcgcggacac atggtcactg cctatgaaaa tgtcacatta
    19741 tggcatgaaa gagatatttc tcattcttca gcagaacgaa tcatcttgcc tgataccacg
    19801 attttattga attacatgtt aaatcgtttt ggcaatatcg ttaaaaatct aacggtcttt
    19861 cctgaaaaca tgaagcggaa tatgtctgca acatttggtt tgatttatag ccagcgcgtt
    19921 atgctgaaat tgatcgataa gggaatgaca agagaagaag cttatgatct tgttcagcca
    19981 aaaactgcgt acgcttggga caaccaagtg cagttccgcc cgctgttaga agaagatcca
    20041 accatcacat cggtattatc taaagaagaa atagacgatg cctttgatta tcatcatcat
    20101 ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg aataaagaag agtgagttgc
    20161 aaaattcaat taaaatagaa tagcaaaaat aaccaaaaaa gaagcagatc cgttagcggt
    20221 tctgcttctt ttagtttatt atattttctt atcaataata ggaacctgat tttaaaagcg
    20281 gctacctctt taccgtgcct ctttacatac aaaaaataga ggaattaagc gtaaatggct
    20341 tggctattat aactaaaatg ctggttatgg agatgttgca gcaagttttc gagaaaagcg
    20401 tattccatct cttgtccatt gaataagatc tggatacgat aatgattttt gaaacgcttc
    20461 tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt ctgtctgata aagaaaagtc
    20521 cttttacggc attcagcaca gcgcttggtt gctcggcgga tttttcggaa tgtataaaaa
    20581 ctagtatctt tattgcataa ataaacgtct ttgatcgaca gcaaatcatt atatagacag
    20641 gaacgttctt cagctgttaa ttctgataaa aaaagctgac cttgttttat tgcatcttcc
    20701 atcggcaagt gcctcctcga acccattatc caaattttta gttaattcta atataccatc
    20761 attaagtata aatgtaaacg cttgttgatg gtataagatt ttcgtttctg ataaaacacg
    20821 aacattaaaa gaatctaatg ccttaaatgt acatattttt gttgatttat aaaaagatga
    20881 ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg tatttgcttt taatgtaaat
    20941 aaaaaggaga gacgtacatg gagaagaaag aacttttgta tgaaggaaaa gccaaaaaaa
    21001 tatatcttac ttctgatgag aacttgattt ggatagaata tttagatcaa gcaactgcgt
    21061 tgaatggcgc gaggaaagac cagataagtg gaaaaggaga attgaataat caaatcacaa
    21121 gtgcaatttt ccgttatctg actcaaagag gaataaaaaa tcattttatt gaacaattgt
    21181 ctaaaaccga acaattagca aaaaaagtac aaattatccc tctggaagtg gttatccgaa
    21241 atattgcagc aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa gaattcatca
    21301 aaccaatcat tgaattctac tataaggatg accagttgga tgatccattt atcaatgaag
    21361 agcatatcta tttcttacaa cttgcaaaac cggaagaaat cagtgagctg aaagcccaag
    21421 cgttggctat taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg gttttagtag
    21481 atttcaagct agaatttggc cggtcgaatg gacaaatttt gcttgcggat gaagtaacac
    21541 cggatacttg ccgattatgg gatgaacgta cgaatgaaca tatggacaaa gacgtttacc
    21601 gcagagatct aggtgatttg atcccagtct atcaagaagt atataaccgt ctatcggaac
    21661 taactgaaga aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa cctataaaga
    21721 ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca gtccatcgta tgggatatga
    21781 aacaatcgaa gatatccgaa taggaaaata ctttgagata caacttgctg atgcagaaga
    21841 accaatcgat acagtgattg aaacgatttg tgacaagctg cttgcaaatg taaatatgga
    21901 aacataccga tatgaaatca aaaaaatgga ggaagtataa catgaaattt gcggtcatcg
    21961 tttttccagg ttcaaattgt gacgcagata tgttatgggc agttcgcgag atcatgggcg
    22021 ctgatgccga atttgtacgg catgatgagt cctctttaga aggatttgat ggggttttac
    22081 tgccgggagg attctcctat ggtgactatt tgagatgcgg tgcgattgct cggttttcta
    22141 caatcatgga agaagtgatt cgttttgctg aagaagggaa accagtattc ggtacatgca
    22201 atggattcca gatattgaca gaagcaggac ttttgccagg tgtactgaga cgaaatgagt
    22261 ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg gcctttactt
    22321 cagagtatga agccgacgaa ataatctatc ttccagttgc tcatggggaa ggaaattatt
    22381 tctgtgacga agaaacactt caagaactaa aaaaaaataa tcagattgcc tttacttata
    22441 atgacaacat caacggcagc gtggaaaaca ttgcagggat tacgaacaaa gcagggaatg
    22501 tgttaggaat gatgcctcat ccagaacgtg cagtagaaga cttgctagga tcagaagatg
    22561 ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta tagatgaaaa
    22621 cattgaaaga accgacagct gaagagatca agaaaaaaag aatctatgcc gaatggggat
    22681 tgactgatga agaatatcga atgatcgaag aagatatctt aggaagaatg cctaattata
    22741 cggaaactgg actgttttct gttatgtgga gcgaacattg ttcctataaa aattctaaac
    22801 cggttctacg caaatttcct actagtggac ctcatgtgtt gcaaggccct ggagaaggcg
    22861 caggcatcgt cgacatcggt gatggactag cagttgtctt caaagcagaa agtcacaatc
    22921 atccttcagc cgtggagcct tatgaaggag ctgcgacagg agttggcgga atcatcagag
    22981 atattttcag catgggggct cgtccaattg cgttgctaga ttcattgagg ttcggcgaat
    23041 tagataatcc aagaacgaaa tatttactag aagaagtagt tgcaggtatc ggcggatacg
    23101 gcaattgtat cggcataccg actgtaggcg gcgaaattgc atttgatcct tgttatgaag
    23161 gaaacccatt agtcaatgca atgtgcgtag gtttgattga acacaaacat atccagaaag
    23221 ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca ggtcgcgacg
    23281 gtatccacgg agcaactttt gcctcagaag aatttaatca agaagaagaa cagcaacgtt
    23341 ctgctgtaca agtaggcgat ccttttatgg aaaagctgct gcttgaagcg tgtttggaat
    23401 taatcttgca tcattcggat tctctaatag gtatacagga tatgggagca gcaggacttg
    23461 tttcatctag tgcggaaatg gcttcaaaag ctggctcagg acttatattg acattagatg
    23521 aagtcccaca aagagaaact gggatgacac cttatgaaat gatgttatcg gaatcacaag
    23581 aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa ctatttcaga
    23641 agtatgatct agatgcagtc actatcggta aagtaacaga cgacgggcaa tatcggctat
    23701 atcatcaagg agaagaagta gcaaatctgc cagtcgatgc attagctgaa gatgcaccag
    23761 tttatcataa agaaatgaaa gaaccagctc gaatcgtaga atttcagcag ttagctcctt
    23821 atcagccagt tatcgaagaa cctggacaag tattgcttga tctgctgcag caaccgacga
    23881 ttgcttcaaa acgttcaatc tacgaaactt atgattcaca agtacaaacc aatacagttg
    23941 tccgtccagg aagcgatgct gctgtgatga gggttagagg gacgaataaa gcgttagcta
    24001 tgaccacaga ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga ggacaaatag
    24061 cagtggcaga agctgctaga aatattattg ctagcggtgg taagcccttg gcgatcaccg
    24121 actgcctgaa ctatggttct cctgataaac cagaagtttt ttgggaatta tcgacttcag
    24181 ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc tgttatttca ggaaatgtct
    24241 ctttatataa tgaaacggat ggtcaagcaa tctatccaac accaatgatt ggtatggtcg
    24301 gattgattga ggatcataaa catatcacta ctcaggaatt caaaaaatca ggggatctaa
    24361 tctatatatt aggtaagacg tttgcagatt ttgatggcag tgaattacaa aagatgcagc
    24421 ttggaagaat cgaaggtgtg atccgaaact ttgatctatc cattgaaaag agaaaccaag
    24481 agttagtatt gacagccatc caaaacggat tgattgaaag tgctcatgac tgttcagaag
    24541 gtggcttggc aattgcactg gcagaatcag ctttcaaaca tcagctaggg atatctgtac
    24601 agtttgaatt gtcctctgct caattatttg cagaaactca atcaagattt gtgttgacag
    24661 tcgcaccgga aaataagaca cgattcgaag aaatgatggg agatgctgct gttttagcag
    24721 gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag atcaaaatag
    24781 aaactgcagt agcaagaaag tgctgggagg atgcaatcgt atgtcttatg aagtgaagag
    24841 tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat ccagacgccg ctagtgtggc
    24901 atacttcggg ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta tcgtgtccaa
    24961 tgatcgagga aagcttaagg ggcatcgaga tttggggctg ctttctgagg tattcaaaga
    25021 tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc ggacatgtac gttatgcaac
    25081 agcaggcaat ggaagtgtgg acaatattca gccttttcta ttcaaatttc atgatcagtc
    25141 gatcggctta gcgcataatg gtaacttgat caatgctcgt tcccttagaa aagagttaga
    25201 ggaaaatggg gcgatcttcc attctaactc cgatacggag atattgatgc atttgatccg
    25261 acgaagtgaa gaaacagatt ttttaaataa attaaaaagt gctttgaatg aagtcaaagg
    25321 tggctttgcc tatttattaa tgacagaaac ggcaatgatt gccgctttgg acccaaatgg
    25381 ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca tacgtgattg cctcggaaac
    25441 ttgtgcatta gaagttattg gggcacgttt tataagagat gtccagcctg gtgagatagt
    25501 gatcatcaat gacgaaggaa tccagattga ttgctttacg aaggacacgc agctttctat
    25561 ttgttcgatg gaatatatct attttgccag accagattca aacatcgcag ggatcaatgt
    25621 gcatactgca cggaaaaata tgggacggaa tttagcgaaa gaatcgccag taaaagcaga
    25681 tatggtcgtt ggcgtaccta attcttcttt gtcagccgca agcggttatg cggaagctag
    25741 tggaatccct tatgaaatcg gattagtaaa aaatcaatac attgcccgaa catttatcca
    25801 gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg tacgaggagt
    25861 agtggaaggt aaaaaggtga ttatggtcga tgattcaatt gttcggggta cgacaagcag
    25921 acggatcgtc catcttttga aagaagcagg ggcaaaagaa gtccatgtga ggattgcttc
    25981 tcctcctttg aaatatccat gtttttacgg gattgatatt caaacgcgta aagaactcat
    26041 tgcggcaaat cacacgattg atgaaatcaa agaatgtatc ggtgcagatt ctttaggttt
    26101 tttaagtgaa gagggtctga tcgaatcgat cggtctggaa aaagatgctc cgtattcagg
    26161 attgtgtatg gcttatttca acggagatta tccgacgccg ttatatgact atgaagaaaa
    26221 atatcaggaa tcactaaaag aaaaagtatc gttcttttaa aagcagacca agaagggagt
    26281 atatgaattc aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa gctggatatg
    26341 aagtagtaga acggatcaag aagcatgtca aaaaaacgga acgtttaggt atgatgggcg
    26401 ctataggagg attcggcggt tgttttgatt tatcggtctt tcaattaaaa gagccagtcc
    26461 ttgtctcggg gaccgatggc gtggggacga aattagcagt ggcaatcgaa gcagacaaac
    26521 acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta gcacaaggcg
    26581 cagagccgtt atatttctta gactacttag caacggggaa aaatgatccg ttacgtttgg
    26641 aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg aaatatggcg ctgattggcg
    26701 gagaaacagc tgaaatgcct ggtatgtact caggtaatga ttatgatcta gccggatttg
    26761 ctgtagggat agctgaaaaa tctcaattat tgacaggaga aacgatcaac ccaggagatg
    26821 tgttgcttgg aatagcatct agcgggattc attcaaatgg gtactctctt gttcgcaaaa
    26881 ttttctttga gcagcagcag ttttctttat catcaaaact gccagaattg aaaaaaacac
    26941 ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc ttccttgctt ccgctgatca
    27001 aagaaaagaa aataaaagga gctgcacata tcactggtgg agggtttgta gaaaatatcc
    27061 cgcgaatgtt accagaatct atggctgcac aaatcaaact tggctcatgg ccgattcttc
    27121 caatattcgg ctgtttacaa aaatatggtg aaattcctga gatggagatg tatgaaatct
    27181 tcaatatggg catcggtatg gtcctggcag tcgaagaatc gaaagtaaaa gaaatagtac
    27241 acgaaattga gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc gccaaagaaa
    27301 aacatcgtgt ggtgtttata ggagaagaac gatgagaatc gctgtttttg cttctggaaa
    27361 cggaagcaac tttcaagcgc tagccgatta tctatcgaaa aaaggattgg aatcttcgat
    27421 tgactggttg ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa ctgcgttgag
    27481 tgtaccggca gattgttttt cacctaaaga atttgattca aaaaaagagt atgaagaagc
    27541 aattttgcat aaattgaaag aaaaaaagat cgatttgatc gtattagctg gatatatgcg
    27601 aatcattgga ccggtattgc tgaaaaatta tgataaaaga atcatcaata tccatccgtc
    27661 tcttttacct gccttccctg gtttgcatgg catacgtgat gcctttgaag ctggagtaaa
    27721 agaaacgggt gtcacaattc attatatcga ccaaggtgta gatactggac caatcatccg
    27781 gcaggaaaaa gtgagaattg aacaggagga cacgttcgat tctttagaag aaaaaatcca
    27841 tagagtagag catcgaatct atccagaagt gattagtgaa attattgaaa atggaggcta
    27901 tctgaaatga caagagcatt gatcagtgta tcagataaaa ctgggattgt agcattcgcc
    27961 caaggtttga ttgaacaagg agtagaaatc atttcaacag gaggaacaaa gcatgcgttg
    28021 gataaagctg ggatcaaaac gctttcgatc gaagaagtga cagggtttcc tgaaatgatg
    28081 gatggccgtg tcaaaacact tcatcctaaa atccatggtg gtcttttagg aagacgcgat
    28141 ctccctagcc atatggaggc aatggaaacg gaaaatatcc aaccaatcga ctttgtttgt
    28201 gtcaatttat atcctttcaa agaaacgatt atgaaaccag atgtcgaaat agcagaagct
    28261 attgaaaaca ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa aaaccatgaa
    28321 tttgtcacct ctgttgttga tccatctgac tatgaagaag tactttcaga attacgtcag
    28381 tatgggcata cttcactttc tttcagacaa aaactagcgg caaaagtctt tcgtcatact
    28441 gcggcatatg atgcactgat cgctcaatac ttaacggatt gggtggaaga aaaagaaccg
    28501 gaaaaattga cacttacgta tgaacgaaaa caaacgcttc gttacggtga aaatagccat
    28561 caagccgctg ccttttatca gtccgctctt ctggaaaaat attcgatcgc agcagcaaaa
    28621 caactccatg ggaaagaact ttcttataat aatatccgag atgcagatgc tgcgctacgg
    28681 atcatgaagg aatatgacga gccaacggta gtcgccttga aacacatgaa tccttgtggt
    28741 attgggacag catctacgat tttagaagcg tgggaagcag cgtatgaagc agatcctgtt
    28801 tcaatctttg gtgggatcat cgttttgaat cgagaagtag atctgccaac agcagaacag
    28861 atgcataagc tgtttctaga aatcatcatt gccccttctt atacacaaga agctttggaa
    28921 ttactgcaaa aaaagaaaaa catccgatta ctgacagtag actttgcgca ggaagaaaat
    28981 caaaatcgag aagaaacggt ctcagtctta ggtggacttc ttgtacaaga tcaagatctg
    29041 gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac tccagcagaa
    29101 gcaaaagcat tagcgtttgc ttggagagct gtcaaacatg tgaaatctaa tgcgatcgta
    29161 ctggcaaacg aacgtcagac agtaggaatc ggagccgggc agatgaatcg agtcggttca
    29221 gtaaaaattg cgattgaaca agcgcaagag aaaatggaag gtgcagtttt agctagcgat
    29281 gcttattttc caatggatga ttcggttgaa tatgcagcca aacatggtat aaaagcaatc
    29341 attcagccag gaggaagcat caaagaccaa gattctattg atatggcgaa taaatacgat
    29401 ctagcaatgg tctttactga tgttcggcat tttagacatt agagaggagt cggataaatg
    29461 aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa acttttagcc
    29521 agtccgaagg tggatacggt gtattgtgcg ccaggaaatc cggggatgac aaaagatggt
    29581 atcaattgtg tcgctatcag tgagcgagat cattcagcgt taatccattt tgttaagaaa
    29641 gaggctattg attggagtat cgttggtcct gaagttcctt tgttaaatgg gatcgtcgat
    29701 gattttatgg cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc tctgatcgaa
    29761 ggttcgaaaa gttttgctaa acaattaatg gatacttatc aaatcccgac agctaaatcc
    29821 agaagttttg aatgttacga aaaagcagca gcgtatatca aagaaaatgg tgcgccaatc
    29881 gtagtgaaag cggacggctt agcagctggg aaaggtgtag tcgtggctac cacagtgaag
    29941 gaagcgctgg atgcggcaga acaaatgttg aaccagcatc gatttgggga tagtagcaaa
    30001 aaaatcgtcg tagaagagtt tttggcagga gaagaattct cccttttggc gtttgtccgt
    30061 aataccaagg tgtatccgat ggtcatttcg caagatcata agcgggcatt tgataacgat
    30121 aaagggccaa atacaggcgg aatgggtgct tattcaccag taccgcaaat ttcaaaaaag
    30181 atgattgaaa aagctgtgga agaagtttta aaaccagcgg ctttgggaat gaaagaacta
    30241 ggacgctctt tcacgggtgt tttatatgcg ggtttgattg ctacgaagga aggaccaaaa
    30301 gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct ttctcggttg
    30361 aagagtgact ttgcgcaagt aatcgatgat ttattagaga atcgtacagt agagttaaag
    30421 tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta ccccgaagaa
    30481 tatgagacag gaatggttct accagatttt ctggaagaag agctttctgt ttactatgct
    30541 ggagtttcct caaataacgg caaactagtt ggagcaggag gacgtcttta tttagtagaa
    30601 gcatacggtg agacgatcaa agaagcacag aaaaaaattt acaccgcgtt aatggataaa
    30661 acgacagatg gaacatttta tcgtacagat attggagaaa aagctgccaa tagcgaactg
    30721 atcgtgaagt aaatcagctg cttccattta taaaaaatac aacaaacata atcggaagcg
    30781 ccgattttag tgaagccttt tcataaagag tgtggtacac ttttgttgtg tatataaata
    30841 aggaggagtt cagatgaaaa aaattcaatt aggtacttct gatcagaaag cagctgcagt
    30901 gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga ttgagacagc
    30961 ttatgaaaac ggcattgatt tttttgacca tgcagatatt tatggaaacg gtgaatgtga
    31021 agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc tttttattca
    31081 aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac atatcattga
    31141 atctgtagaa aaaagtttga cccgtctgaa aatggattat gtggatgctt tacttcttca
    31201 tcgtccagat acattggtag aaccagagga agtagctgaa gcgtttgatg aattggaacg
    31261 gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa tcgaattgct
    31321 gaaaaaatat gttcgtcaac cattagtagc taatcagctg caatttggct tgaaacatac
    31381 tggaatgatc gatcaaggga ttcatgtcaa tatgacagat gaagcaagta tcgatcatga
    31441 cggtagtgta cttgattatt cccgattgca tgatatgacg atccaagcat ggtcaccata
    31501 ccagtatgga ttttttgaag gtgtatttat cgggaacgaa aaattccctg aattaaacca
    31561 aacattagaa gaactagcag ctaaatatca aacgacacca actggcttgg cttctgcttg
    31621 gattttacgc catccagcca atatgcaagt gatcgccggt acgatgaacg tagggcgaat
    31681 caaggaaatc gctcaagctt cagaaattaa tatgtctcgg gaagattggt acgaattgta
    31741 ccgtgctgct ggaaacattt taccttaaag attgtcataa gcaggtcaaa agggctataa
    31801 attaaaaaga cagaataact tgaaagctgt tttcagcttc gagatattct gtcttttttt
    31861 attgcaaatg gaaagagtat cattgtacgg atttcgttta ttagttattt ttgtttttaa
    31921 ggaaaagctg tgtccgatat tcttttggag aaatcccttg ttttttctta aataaataac
    31981 tgaaatagtt agggtcatta taaccaattt caaaagcaat atctgaaata cgatagtctg
    32041 attgctgcaa taattttttt gctaattcga ttctttgttc tgtcagataa tcgatgaatg
    32101 tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga gggacttaat gcaacttgtt
    32161 gagcgaccca agttaaggac atatctggat cggtaaaatt ttctttgata aaatccaatg
    32221 catggttcaa aattggctga tatttgttcc ttgaaggctg tgagggcaga gcagttaaga
    32281 aagaaatgaa catataaact gtcgaacggt aaagcgagtc gtcggcgctg attgctaagc
    32341 gttgactggt ggatgtgcaa tgaaccattt cttgcgggat tttcttttct ttcaggcaga
    32401 catgctggag tagcttagtc aagacaaata aacgatataa tcgtgttcga gttggtgtgc
    32461 cttgtggttt cgtcaagtgt tcgatgtact tagatacttc gtttttcgat aatgaagaca
    32521 aatcaagtga gaggtcaaag taaaagacag aatcaactgg aagtttcgct tcttgaatca
    32581 aatcctcata gctgataatt tgttctttaa gggaactggc ggtttcaagt atcatctgcg
    32641 tgatctcaaa agatgcaggt aattcgctga ttctttctgc gacaggacca atggcgacag
    32701 aaaaattatt tttttgtccc ttttctaatt catggaccaa cgtttgggct acctgataac
    32761 tggtctcgat cacattttct tcaagagaat caactattaa aaatttgatg aatcgagagg
    32821 aaacagcaga gaagatcact tgttgattct ccctaaataa aaacttcaaa taatcactga
    32881 gatgagcgta atcttcaaag tctgtactga cttggtttgc tgctaataat acggtcattt
    32941 ttttccctgc taaagagcga ttgaaagatt cagcaagttt caatgtttca ctgatcgtaa
    33001 gtgagccttt gaacaaaccg ttcaagaaat gatttttttt caattcgaaa acaagatcag
    33061 aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt tactgctttt tctattgctt
    33121 gtttcaattc gctactttta atcggtttaa gcagataatc atctgcctgt acctggattg
    33181 ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa tatgagacga gtccaaggaa
    33241 gtagtttcct tgcttctttt gaaaaagtca acccgtccat gaaaggcatt tggatatcag
    33301 tcagaatgat gtcgggctgt aagtcgataa ttgaagacag tgccatctca ccatctccag
    33361 cttcgccgac ataagtgatt ggataataag aaccaaggac agttatttgt tcccgcaagt
    33421 tattgcgaat gaggtattcg tcttcgatga taaaaacttt atacatgttt gtttcctcca
    33481 attgttttgg gagatagagg gatcatcagt gtgacagttg ttccttctcg gtatttgctg
    33541 tcaatcgtga taggtttttg atcatttccg taataaagaa ggagacgctt gtttacgttg
    33601 taaagtccgt agccgatact gaaatctgaa tcgatccctt tggctaactc tttacgtatt
    33661 tctgccagtt tttctgcagg gatgccaatc ccgttgtcta taactttgaa ttggatcatc
    33721 tcatcaattt tgcatgcggt aatacgaata aatcctttgt gacggacgta cttcgttcca
    33781 tggtatacag cattttcaat caatggctgc aagatcattt ttaaaataga aacaggaaaa
    33841 agtgtttctg gtatatcgat cttatagtca agcatatggc cgtatcttat ttttaagatg
    33901 actagataat cattgatatg gctaatttct ttttcaagag gaatccagtc ttgtcctttg
    33961 cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca tagtcagagc ttcttcaaag
    34021 cagtcttgtt cgatcaaaga aataatggca tccaaactgt tataaataaa atggggagta
    34081 atctgagctt gcaatacacg aacttcactt tgtgctaaat ggtattgttt caaagcgttc
    34141 tcccgtaaag actggtttag atcttccgcc attttattca tgctagcggt aagtgtttgt
    34201 aattccagtg tttccggcag ggttaaacga aagccaaggt tccctgaaga aagttgttct
    34261 gacatctcga tcagctgatt gatcggttct tggacctttc tctctaaaaa gcgacgattc
    34321 tttttcgcat agaaaaaaat ggtactaaga actccaagtt ccactattgt taatagaatg
    34381 agcgagcgga caagtgtcgt attttttttg cttgctaagt cgatctccac acgaacaaaa
    34441 tcttgtaaga tatctgacag cagttgagtg acagaatcta cctgattgat gatctcttca
    34501 ttggtatcca taaaatcttt tgccaagata ttattgatca gctcgttttg ataatcttcc
    34561 agtgtatcga tcgtacgtag cgatacatct aacgtactaa tttctttaag tgtatttgta
    34621 ttatttttga tctctcgaat atcattccta agttcttcta ggatattttc ctcattgaac
    34681 tcatctacgg ttacttggcc agaaatcaag tcccacatac tttccaaaac tttttgatca
    34741 accttcaaag caattgcatc tgctttttgg atatttgcga tcgtctgttg ataaatcaaa
    34801 agctgttgcg aatataacag tatgctcagt agcaaaggga taacagcaaa tagaagcatg
    34861 gctttgtttg cttgggcaaa aatctgtttt atgcttaaat tttttgtcat gatacttctc
    34921 cagtcaataa tttctggttg ggatgttagc tatattctgc tgggaaaaga cagtctcgga
    34981 aataaaaata tctttaggta atgtttttcc tgtttgaata tttccaaaat aacgagtaac
    35041 ggtattggct acataccagc cgacatcagg tttacactcg actacacaat tgacgattcc
    35101 tgagcgtagc ttctcgatca tatcttcttg tccatcaata gttacaatga tcaaatcttc
    35161 tccaggtttg attgatgttt cttttatcgc agctaaagca ccgtgagtca tttcatcact
    35221 atggctgtat aatacatcga tttcggataa atcatttgtt tctaaatatc gtttcatctg
    35281 gtcttttcct ttcatccgaa taaaatcacc gacaattcgg tgaacaatag tcatcggagg
    35341 tttatggtcg atggttccat ctttttttag ggttggttta cgattgattg attccaaaaa
    35401 tccttcggag cgtagcttgg tcggtgtgga gttctctgaa ccagcaagtt ctaggattct
    35461 gatttcctgt ttctttgaat ttgtgaagtg attgctgaca aataaacccg cccgatttcc
    35521 ttctgctttg aaactggggc caatgtgggt gacgaaaagc tcgggatcat tcgtttgaat
    35581 atgtctgtcg acaagtatga cagggatccc tgctcgcttt gcttctttta atacagagtc
    35641 ccacccggat tcttgcaaag gagtaaaaat gatcatatcc actttatagg caatgaacgt
    35701 ccgtatatct tggatttgcc gttcctgatt catgtagcca tttcggtaga gaacttggta
    35761 atcttctttt tcaagcgcag tgataattga ttcagtatgc tttttccgcc agctactctc
    35821 cgttcctgat tgcgaaaaac caacgactag tgggtcaggt gcttcctttg tattaggtgt
    35881 acaccccatc atttggtaag cgattacaac aagggctaaa aaaaataaaa cacgtttcac
    35941 tttaaatcac tccatctgga tttgttagta ctatcttatc agataaaagg gaaaatcaaa
    36001 gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa aaaatccaag acaaaaaaag
    36061 tacacctaaa aaatttaaag taaatcccat aaaaaaatca atgttaacgc tttctttatt
    36121 agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa tgaaaaacaa atttagaaag
    36181 attgttggga ctatcgctat tttgaccgca agcattctat taagtgcgtg tggcaattca
    36241 ggatctgcag atgatagtac ggggtatgta ggaatcgcta tgccgaccaa atcagcagaa
    36301 agatggatcg ctgatgggaa caacatggtc tcggaattag aaaaactggg ttataaaaca
    36361 gatcttcaat atggagaaga taaagtagaa aatcaagtag cacaaataga aaatatgatt
    36421 acaaaagggg tagatacact tgtgattgct tctatcgatg gttcggcttt aactgatgtg
    36481 ttagctaaag caaaagaagc agatatcaag gtgatcgcct atgatcgttt actgatgaat
    36541 tcagaaaatg tcgattatta cgcgactttt gataattttg gagtaggtgt atcgcaagca
    36601 gcttatattg aagagcactt aggattgaaa gaaggaaaag gaccattcac gattgaatta
    36661 tttggtggct cgcctgacga caacaacgca ctaatcaatt acaacggagt gatgtctgtt
    36721 ttacaaccat atatggacaa cggtcagttg gtcgtgccat ctggacagac gagtttcagt
    36781 caaatcgcta cgcttcgctg ggatggttct acggcgcaag caaggatgga taatctgtta
    36841 agcgcaaatt atactgacca aacattggat gctgttttgt cgccatatga tccaataagt
    36901 ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga gtgaaagtaa acctttacct
    36961 gtgattactg gacaagatgc gacagttgct ggcgttaaat cgatcattgc cggggaacaa
    37021 acacaaacga tctttaaaga cacacgtatc ttagcaaaaa atacgattga aatgatcaaa
    37081 gcaatcagtg atggagaaga ggttccggtc aatgatactg aaacttatga taatggggtg
    37141 aaaactgtcc ctacgtattt agcaaataca gtatctgtcg ataaagataa ttatcaagca
    37201 gagctgatcg acaccgatta ttataaagaa tccgatctga aaaattaaga ggaaccaaac
    37261 aaggaactaa agagatctaa gtcagagaga ggataagaat tatggcggat tgtattttaa
    37321 aaatggcaga tatcaccaaa aaattttctg gaatccgagc gttagattgc gtgaatctga
    37381 tgattgaacg tggggaaatc catgcattgt gcggagaaaa cggagcggga aaatcaacat
    37441 tgatgaatgt cttgagcggc ttgtatccat atggctccta tgacggggaa atcttttatg
    37501 atggaaagct gtgtcaattt aaagatttga aagatagtga agaaaaaggc atcgtcatca
    37561 ttcatcaaga attagcacta agtccttatc tttcaataaa agagaatatt tttctcggaa
    37621 atgaacaagc agaaagaggc gtgattgatt gggaactgac agaaaagaaa acatatgaac
    37681 tgctgaataa agtcggtttg tctatcgacc cgaatcttct agtatcccag attggcgtag
    37741 ggcaacagca actagttgaa atagctaaag cattatcaaa atccgtccgt ctgttgattt
    37801 tggatgagcc taccgctgca ttgaatgaag aagaaagcgc gaatttgctg agattgatcc
    37861 gagagttgaa agagcaaggt gtaacctcca tcattatttc acataagctg aatgaaatcg
    37921 tggatatcgc agatcgaatc accattttac gggatggaca gacgattgag acactagaaa
    37981 atgaaatgat cagcgaagag catattattc gcgggatggt gggcagagat ttagttgatc
    38041 gttatcctga aagaaaaccg gatattggag aagtttattt taaagtagaa gactggacgg
    38101 tctatcaccc tcttgatcac gagcgaatca taaacgacca catcaacttg catttgaaaa
    38161 aaggagagat tgtaggaata gctggactga tgggagcggg cagaactgaa ttcgctatga
    38221 gtctatttgg acattcttat ggaagcaaga tatccggacg tatattaaag gaaggggcag
    38281 agatatcagt taagaccgtt ccttcagcca ttgcaaacgg actcgcttat gtctcggagg
    38341 accgaaaatc tttaggactg aatctgctga tggatattcg ggaaaacgtg acgctgagca
    38401 gtttggataa aataagtcaa aaaggagtat tagataaaga aaaagaagta actgaggcag
    38461 aaaatttccg gaaaaagatg cgtataaaaa caaactccat ccatcaaatt gtcagtagtc
    38521 ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg gctgatgaca caacccgata
    38581 ttcttttttt ggatgagcca acccgtggga tagatgttgg ggcaaaatat gagatctaca
    38641 cgattatcga agaaatggca gcaaatggca aagctgtctg tcttatctct tctgaacttc
    38701 ctgaaatatt agggatgtgt gatcgaatct atacgatgaa cgaaggacgt attacaggag
    38761 aagtctcgcg agaagatgca gatcaggaag tcttgatgaa attaatgacg aaagaagagg
    38821 tgggttaagt tggaacaggc aaaaaatctt acggataaaa aagagaaaaa actaaatttg
    38881 aaaagtagag cagctgatct ttttggtcgc tacagcatgg tgatcatttt agcgggacta
    38941 cttattgcgt ttcaattgat gacggatggt attttctggc gtccattgaa tataactaac
    39001 cttgttctgc aaaatagcca tatattagta ctagctgcag ggatgttatt agttgtgctc
    39061 ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt ttgttggtgc aattgcgggc
    39121 atgatgatgg tcaatcgcca aatgaacccg tggatgacag ttatcgcatg tcttgcagta
    39181 ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt atgcagggat accggcgttt
    39241 atcgtgactt tagcaggatt gctgatgttt cgtggactga cacaagttgt tctaggaggc
    39301 caatctctag caccttttcc agctgttttc caaaaaatgt ctacgggtta tttaccagac
    39361 ttaagcgaag gaagcagcct tcacttcttg acattgattt taggaatgat catcacgatc
    39421 gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga aaaatcattt atttgaagtg
    39481 gaatctccaa cagctttctt ggtaaaggcg gtatttacag cagtcgttgt actaggaata
    39541 acttatatct ttgcctctta tcaagggtat ccgattattt tgatcattct tggtttgatc
    39601 gttgcagcat atgggttttt gacaaataaa acagtagcag gtcgacagat ttatgcgacg
    39661 ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa caaagcgaat cacgttctgg
    39721 gtattcgtca acatgggagt catggcagct ttagctggat tagttttagc tgctcgattg
    39781 aatgcggcaa cacctcaagc aggtacatcg atggaactgg atgcaatggc ggcagtatat
    39841 tttggtggtg cttcgacttc cggcggcatt ggaacaatca tgggtgcgat tgtcggtggc
    39901 ttggtcatgg gtgtattgaa caatggcatg tcgattttag gcgtcggggt agactggcag
    39961 caggcaatca aaggattgat cttgctttta gccgttgtgc tagacttgta taataagaaa
    40021 aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa gactgtgaca agacatttgt
    40081 cacagtcttt ttttcgctaa aagcgaacat tcattgtttc gtttgtatag actaaaaata
    40141 atatatgttt ttttaccatt cgtataaatt ctctttttgc tcttgatttt tttgcttaat
    40201 tgagttaaat tacatagggc gctaaaaaaa gccttgatta aattaataat gttcgtgttt
    40261 tgctggatgt gaacatcagg aggataatgt aatgaaagta tttgattacg aagatattca
    40321 attaattcct aataaatgta tcgtaaatag ccgttctgaa tgtgatacaa cggtcacatt
    40381 aggtaaacat acattcaaaa tgccagtagt gcctgccaac atgcagacga ttattgatga
    40441 aacaatcgca gaattcttag cggaaaatgg ttatttctat atcatgcatc ggtttgatga
    40501 agcagctcga attccgttta tcaaaaaaat gaaaaagcgt ggattgatta cctcgatcag
    40561 tgttggcgtc aagaaagagg aatattcctt tatagagaag ttggcagaag aatcgctaaa
    40621 tcctgattat atcactatcg acatcgcgca tggacatgct aactcagtta ttgatatgat
    40681 tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt gccggtaatg ttggtacacc
    40741 ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca acaaaagtcg ggattggtcc
    40801 aggaaaagta tgtattacaa aaatcaagac aggctttggg actggaggct ggcaactggc
    40861 agctcttaga tggtgtgcga aagcagctcg caagccgatc atcgctgacg gtgggatcag
    40921 aacacatggc gatattgcaa aatctgtccg ttttggtgcg acaatggtca tgattggttc
    40981 attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa gtcgaaaatg gaatcgtgta
    41041 taaagaatac tttggttctg cttcagaatt ccaaaaaggc gaaaaacgaa atgttgaagg
    41101 aaagaaaatc tggatccaac acaaaggatc tttgaaagat acactagtgg aaatgcaaca
    41161 agaccttcaa tcatctattt cttatgctgg aggaagagat ctggaagcca ttcgaaaagt
    41221 tgactatgtc atcgtgaaaa attcgatttt taatggagac acaatctaaa aaataaataa
    41281 aatattagga tgttgtgaaa aagttgtcct gcattaagta ataagaacag ccaaacaaaa
    41341 atagttcttt atatgttttg tttggctgga attattatcg cagatacaag cttttgaaca
    41401 tcattattca gaataaggga ctgtgataag atttttgtca cagtccctta tgttttgcaa
    41461 aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa aagaaaaaat agacaataga
    41521 aaagagaagc ttatgtggga aaaagtagag aaaaaacaag gaccgattta tcgtcaaatt
    41581 atggaacaga ttatgagaga aatcgaaaac ggaaggatca atcctggaag tcaacttgaa
    41641 tcagaacgaa aactagggca actttttggt gtgaatcgtt ccaccgttgt ccatgcattg
    41701 gaagagctta gggagcttgg ggtactaacg agcaaacgtg gcagcggatg gtttgttaat
    41761 cagactgaat gggggaaatt cgctgttcca agaatcgatt ggcggcaaat gatctctcct
    41821 cgttatgagc agacggactg gtatgagcaa aaaataagag aagcaaagca gatccaaaaa
    41881 acatcgtttc ttgatttata cgctagcgag atgccagatg agcttttgcc gaattttgag
    41941 tttccttctt attctcttga agaaatctta gcggaagaaa aagaaatgga tcttttaggc
    42001 tatactccgt tacgtaacaa aatcaaacgg tatttggaaa gtaaatttga attggcattt
    42061 tcccccaacc agcttcttgt gactggtggc ggtcagcaag caatcttttt gattttacaa
    42121 acgattcttt cagctggaga ggggatagct gtagagtcac cttctttttt ttaccgatta
    42181 cctcttttca aagcttcagg aacgcgttta tttgggatac cgatggatga agaagggatc
    42241 gacttagaaa aactgaaaga atcgatccat aaaaataaat tgaaagcagt gcttatcaac
    42301 ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa aacgacgaga aaagctagta
    42361 gaattgtgtc gacagtatcg gcttccaatt attgaagacg atgtatttac ggatctttca
    42421 tttctaaacc aagaagaagt ccttcctata cgaacgattg atccggataa tgtgttatac
    42481 gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa tcggttgggt gataggacct
    42541 gagactttta tctcgcaact agctcaagcg caagaaatga tggagttttc gatgaatatc
    42601 cttacacaaa tcacagtggc taatgtattt aatgaagcaa ttgatttgaa aatgcaagat
    42661 gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg cttggggaga ttcacaatcg
    42721 tttttcaagc ttcattcaat tttaggtggt tactatgcct ggattacttg ggatgggaaa
    42781 aaactagacc ccgaactagc agaaaaagta gtagatatgg gtcttggaat tgcaccaagt
    42841 ctactttttg ggcaagatat aaatggaatc agaatcaatt acagtaggct ggatgaaaac
    42901 caaataccgt tgttttattc aaagatgaaa attctagaaa agtggttaac agaataattt
    42961 cacaaagtaa atagattaaa gaatataaca gcttggattt aaatttcaag ctgtttcttt
    43021 tttttcggaa aaagaatacg atataaagaa actaagaaaa aaccattatt tgtacgtccg
    43081 ttcacgatgt gtaaataaaa taaatgtttg caacaaaact gtataacacc aacgttttta
    43141 aatagaaaca gatggttgta agtttgatta gaaaatagaa aaaatgcaga tgaaaaataa
    43201 aacgctttcc ttattgtgat tttattcact aatgctgtta atatgcggat tggcataatt
    43261 tcctttcatc gtgaaaattt gtgcttttag cttgtatttc tcataaatcc atgataattt
    43321 attaatgaag acggttaaat agtgatacag ttttattcac tgtttcaatc gcagaatgaa
    43381 gagattttct tatagatgag agaggaatgt gtgacaaatg gcaaacaaaa aagcaccgat
    43441 cgacttccaa gcactgctag atgaagtcaa tgcagatttc ccagtatatc aagcacttga
    43501 tcaagatgga aacgtagtta acaaagattt agtacctgat ttatccgatg atgaactcgt
    43561 tgaattaatg actcatatgg tatggtctcg tgtattagac caacgttcaa cagcgctgaa
    43621 ccgccaagga cgcctaggct tttttgcacc aactgctgga caagaggcaa gtcagctggc
    43681 tagtgcttac gcatttgata aagaagatgt tcttcttcca ggttatcggg atgtacctca
    43741 actagtaaaa catggtttac ctttatctca agctttttta tggtctcgag gacatgctgc
    43801 aggtaattct tatccggaag aattgaaagc tctaccacca caaattatca tcggtgcaca
    43861 atatgtacaa gcagccggtg ttgcattagg tttgaagaaa cgaaacaaga aaaatgtcgt
    43921 cttcacttat actggtgatg gcggttcatc acaaggtgat ttttacgaag caatcaattt
    43981 cgctggcgct taccatgcaa atgccgtatt ttatatccaa aacaacgggt atgcgatctc
    44041 tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca caaaaagctg tcgcagctgg
    44101 gattcctggt attcaagtcg atggaatgga tccgcttgca gtatatactg tatctaaaat
    44161 ggcacgggaa tgggcgatca gtggaaatgg accagtccta atcgaaacac tgacttatcg
    44221 ttatggacca catacactat ctggtgacga tccaacccgc taccgtacaa aagacacaga
    44281 tgacgattgg cagaaaaaag atccattgat ccgcttccgt aaatatttga cggaaaaagg
    44341 actttggtct gaagaaaaag aagaacaagt catcgaagca actaaagaag aaatcaaagc
    44401 agctattgct gaagccgaca aagtaccaaa acaaaaggtt tcagatttct tgaaaaacat
    44461 gttcgaagta tctccacaat caatcaaaga acaaattgca atttatgaag cgaaggagtc
    44521 gaaataatca tggcacaaaa aacaatgatc caagcaatca cagatgcctt agcacttgaa
    44581 ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg ttgggaaaaa cggaggagtt
    44641 ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag accgagtatt cgatactcct
    44701 ttagctgaat ctggaatcgc aggattgagt ttcggtcttg cgttagaagg atttcgtcca
    44761 gttcctgaaa tccaattctt tggttttatt ttcgaagcaa tggacgaagt agtagctcaa
    44821 atggctcgta caagatatcg aatgagcggc actagaaatc tacccatcac aattcgttcg
    44881 ccatttggtg gcggtgttca tacaccagaa ttgcattcgg ataatttaga aggtttgatc
    44941 gctcagtctc ctggtattcg agtagtgatt ccttccaacc cttatgatgc aaaaggacta
    45001 ttgatttctt ctattagaag taacgatccg gttgttttcc tagaacacat gaaactatat
    45061 cgctcattcc gagaagaagt gccggatgaa gcttacgaag ttcctttgga taaagcagca
    45121 gttactcgtg agggaacaga tgtatctatt atcacatacg gagcaatggt gcgtgaagca
    45181 atcaaagcag cagacaatct tgcaaaagaa aatatatctg ttgaaatcat tgatcttcgt
    45241 acagttgcgc cattagatgt agaaacgatc attcaatccg tggaaaaaac tggtcgcgta
    45301 gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg cgcaagtagt ttctgagatc
    45361 tcagaacgcg ctatcctttc attagaagcc ccaatcggac gagtttctgc acctgataca
    45421 gtcttcccat ttggacaagc agaaaatgtc tggttgccaa atgcaaaaga tatcgaagac
    45481 aaagtaaaag aaatcgcgga attttaagaa aagaagggac gaaacaaagt catggcttat
    45541 caatttaaat tgccggatat cggtgaagga atcgcagaag gtgaaatcgt caaatggttc
    45601 gtcaaaccag gagatacgat caatgaagat gatacattac tagaagttca aaatgacaaa
    45661 tcagtagaag aaatcccgtc acctgttaca ggaacggtta aaaatgtgat tgttccagaa
    45721 ggtaccgtag caaatgttgg ggatgtatta gtagaaatcg atgcaccagg acacgaagac
    45781 aatgaagggg acagtggtgt agccgcagaa tcccaaactc cagctaaacc ggctgctgaa
    45841 cccactgttg atacagaatc agctggatcg tcaagcgaag gagtcttcca atttaagtta
    45901 ccggatatcg gtgaaggaat cgcagaaggt gaaatcgtca aatggttcgt caaaccagga
    45961 gatacgatca atgaagatga tacattacta gaagttcaaa atgacaaatc agtagaagaa
    46021 attccgtcac ctgtcacagg gacagttaaa aacgtgattg ttccagaagg cactgtagca
    46081 aatgttgggg atgtattagt agaaatcgat gctcctggac acaatagtgc accgtcaact
    46141 agtgcgccat cagctgaagc accaaaagaa aaagtggaaa catcaggttc agcaagtgtc
    46201 gttgaagctg ccgatccaaa taaacgtgta ttggctatgc catctgttcg tcaatttgcc
    46261 cgtgaaaaag atgtagatat ctcacaagta actgcaactg gaaaaggtgg cagagtaaca
    46321 aaagaggaca tcgaaaactt cttggcaggt ggtccaagca gtgcaccagc gaaatcagaa
    46381 gcgccagaag cggcagcacc gaaagaagca gctcctgcag cagaaagcaa accagctgca
    46441 ccagcaaaac cattcaagtc caacttagga gacttggaag aacgtgtagc aatgacgcca
    46501 acacgtaaag ctatcgctaa agcaatggtc aacagtaagc atacagctcc acacgtgaca
    46561 ttgcatgatg aagtagaagt gtctaaacta tgggataatc ggaaacgatt taaagaagtt
    46621 gctgctgcaa atggtacgaa acttactttc ttgccatatg tcgtgaaagc tttaacagcg
    46681 actgttaaga aatacccagt attgaatgca tctatcgacg atgcaaacca agaaatcgtt
    46741 tataaacatt actacaatat cggtattgca actgatacgg atcacggact atatgtacca
    46801 aatgtgaaag atgcagaccg caaaggaatg tttgctattg cagacgaaat caatgagaaa
    46861 gcaaaattag ctcatgacgg caaactatct gcagaagata tgcgcaatgg aacaattacg
    46921 atcagcaata tcggatctgt aggtggcggt tggtttacac cagtcatcaa ctatccagaa
    46981 gtagctattt taggtgtagg aacgattgca caacaaccaa tcgtaaatgc tgaaggcgaa
    47041 atcgttgtcg gtcgtgtgat gaaactgtca ttaagtttcg atcaccgtat cgtagatgga
    47101 gcaactgcgc aacaagcaat gaataatatc aaacgtcttt tagctgatcc agagctatta
    47161 atgatggaag gatgataaac aaatggtagt tggagatttt gctgtagaat tagatacagt
    47221 tgtaattggt gcgggacctg gtggctatgt agctgccatc cgcgctgctg aaatgggaca
    47281 aaaagtagca atcattgaaa gagagtatat tggcggtgtt tgtttgaatg tcggctgtat
    47341 tccttcaaaa gcactgatag ctgcaggaca ccattatcaa gagtcactag attctgaaat
    47401 gtttggagta actagtgaaa acgtcaaact agattttgca aaaacacaag aatggaaaga
    47461 aaacaaagtt gtccatacgt tgacttctgg tgttggtttc ctattaaaaa aacataaggt
    47521 tgaaacaatc gaaggagaag cgttcttcgt agatgatcac acactacgcg tcattcatcc
    47581 agattcagct caaacttatt cgttcaataa cgcaatcatc gcaacaggtt cccgtccaat
    47641 cgagattcca ggatttaaat tcggaggacg tgtgttagat tctactggcg gattagcatt
    47701 gaaagaagta cctaagaaat ttgtgatcat cggtggcggt gtgattggtg ccgaattggg
    47761 cggagcgtac gcaaacttag gagcagaagt aacgattctt gaaggctcac cacaaatttt
    47821 accaacttac gaaaaagatc tagtaaaatt agtagaagat gatttcaaga aaaaaggcgt
    47881 aacagtcgta acgaatgcaa tggcgaaaga agcagtcgat aacggtgaca gcgtgacagt
    47941 gaaatatgca gtggacggga aagaagaatc cgttactgct gattatgtga tggtcactgt
    48001 aggtcgtcgt ccgaatacag atgatatggg cttagaacaa gcgggtgtcg aagtaggaga
    48061 acgaggattg atcacggtgg ataaacaagg acgtacgaac gtacctaaca tctatgccat
    48121 cggtgatatc gttccagggg cggcattagc acacaaagcc agttatgaag caaaaatcgc
    48181 tgctgaagca atctctggca aaaaagtagc tgtagattat aaagcaatgc cggcagtagc
    48241 cttcactgat cctgaattgg cttctgtagg tatgacgatc aaagaagcaa aagatgctgg
    48301 tatcgaagca aaagcatata aattcccatt ttcagggaac ggtcgggcat tatcgcttgg
    48361 taaaacagaa ggattcatcc gtctagttac gacgatcgaa gataacgtgc ttattggcgc
    48421 gcaaatcggt ggagtcggcg caagtgatat ggtctctgaa ttagctttag caatcgaatc
    48481 aggaatgaat gctgaagata tcgccttgac gattcatcct catccatctc ttggcgaaat
    48541 cgtcatggat gcttcggaat tagcattagg tttaccaatc catatttgat ttgaataata
    48601 caggcgaaac gcaactgaaa aatcagttgc gttttgtttt ttagaattat caataatatt
    48661 attatgtaaa cttaaaaata tgacaacttc ttggtttatt accacttcct atgctacact
    48721 gaagaggaat atgaaatagg agagttgaag atgcaagaat atcagtatcc acttgattta
    48781 gattggacaa cagacgaaat ggtcattgta atgaagatgt gggaagatct ggaacgtgcg
    48841 aatgaaaaag ggataaacaa tgaagctttt ttgaagactt atcagcagtt taaaactgtt
    48901 gtcaaatcca ttggtgagga aaaacgattg ggacgggaat ttgaaaaagc ttccggttat
    48961 tctttatatc gtacagtgca gcaagcaaaa aaaaatccaa acaaaacttt gagaatgaac
    49021 gggtgagata aatggaacag atgattgatc atatcaagtc ctggcttgaa gaagctggtg
    49081 agaaaatcaa atcttctcaa caaacattaa caatctcgca aaaatcaaat cgaacggatc
    49141 tagtaacaaa tgtcgatcga aacatccaga attttctaat cgaaaaaatc cattcctttg
    49201 atccagaggc gaaaatattg gcagaagaag aagggtgtaa tcaattatcg gatctcgatg
    49261 gaagagtctt tatcatcgat cctattgatg gcaccttgaa ttttgtgatt gaaggggaaa
    49321 atttttgtat tatgctcgct gtttatgaat ccggcgttgg aaagcttggt ttcatttatg
    49381 acgtgatgag aaatgaactt ttttggggtg gaaaaggaat aggcgtgtat aaaaatgatg
    49441 agccattgcc tagaccagca aataaatcct tatcggaagg cttacttggt gtgaatagtt
    49501 atcttttcgg tcataataaa tatggaatcc gagaaattgc ggaaagcagc atggggacac
    49561 gtatgtacgg atgcgctggt cttgaactag tggcaatctt gaaaggaaac cacattgctt
    49621 atatttcaaa ccttagccct tgggattatg cagcgggaaa tgtcttattg gaagagtttg
    49681 gtatggtaac aagcggtctt tttggtactc ctttgacatt tgaaaaaagg gaatactatt
    49741 tagcagggac ttcttgtgca tatgcagaaa tcgcagacct tttgaaaaaa gaaggctgaa
    49801 aaaaaggtag aaatcattca caaaaatgat aaaaacgagc aaaagattaa ttttctcgaa
    49861 aaaatgaaaa cattttacta tatttcatga aaaaagtctg ctataattag cagtcgagta
    49921 aaatttgccc tgtcataaag aaccaataaa aaaggagtta gttacattga attatagaaa
    49981 cgatatccgt aacgtcgcaa ttattgccca cgttgaccac ggaaaaacaa cattagttga
    50041 tgaactatta aaacaatcag atacattaga cgcacatacg caattgcaag aacgtgcgat
    50101 ggactcaaat gcgctagaaa aagaacgtgg tatcacgatt ttagccaaaa acacagctgt
    50161 agactataaa ggtattcgcg taaacatcat ggatactcca ggacacgcgg acttcggtgg
    50221 cgaagtagaa cggatcatga aaatggttga cggcgttgtg ttggtcgtag atgcgtatga
    50281 aggaacaatg cctcaaacac gttttgtatt gaaaaaagca ctggaacaac atatcacacc
    50341 aatcgttgtc gtaaacaaga ttgacaaacc ttctgctcgt cctgagcatg tcgtagatga
    50401 agttcttgaa ctattcatcg aattaggagc agatgatgat cagttagact ttcctgtgat
    50461 ttatgcatct gcactaaatg gtacgtcaag cttgtcagat gatccagcag atcaagaacc
    50521 aaccatggca cctatttttg atacaattat tgaaaaaatc cctgctcctg tcgataacag
    50581 cgacgaacca ttacaattcc aagtttcatt acttgactac aacgattatg taggtcgtat
    50641 cggaatcgga cgtgtattta gaggaacaat caaagtagga gaccaagtag cattaattaa
    50701 attagacgga acagttaaga aattccgtgt aacgaaatta tttggtttct ttggactaaa
    50761 acgtctagag attcaagaag caaaagctgg agacttgatt gctgtctcag gtatggaaga
    50821 tatctttgtc ggtgaaacag ttactcctgt ggatcatcaa gatgcgttac ctatccttca
    50881 cattgacgaa ccaacattgc aaatgacttt cttagttaat aattcaccat ttgccggtcg
    50941 agaaggtaaa ttcgtaaccg ctcgtaagat tgaagaacgt ttgatggcag aacttcaaac
    51001 agatgtatcc ttacgagttg aaccaacaaa ctctccagat gcatggacag tttctggtcg
    51061 tggagaattg catttgtcta tcttgattga aaacatgcgc cgtgagggat acgaattgca
    51121 agtatctcgt ccagaagtta ttgaaaaaga aatcgacggt gtaaaatgcg aaccgtttga
    51181 acgcgtgcaa atcgatacac cagaagaata tatgggtagt gttatcgagt ccttgagctt
    51241 acgtaaaggt gaaatgcaag atatggtcca tacaggaaac ggacaaatcc gtttgacatt
    51301 cttaacacct gcccgcggtt tgattggtta ttcaactgaa ttcctttcaa tgacacgtgg
    51361 ttacggaatc atgaaccata cttttgacca atatctgcca atgcttcctg gtcaaattgg
    51421 cggacgtcac caaggggcgc ttgtttcgat cgatacagga aaagcaacga cttattctat
    51481 catgagtatc gaagaacgcg gtacagtctt cgtagaacca ggtacagaag tatatgaagg
    51541 aatgattatc ggggaaaaca gccgtgataa tgatttgact gtcaacatca cgaaagcaaa
    51601 acaaatgaca aacgtacggt ctgctactaa agaccaaaca tctgtcatca aaaaaccaaa
    51661 acaattgacg ttggaagaat ctttggaatt cttgaatgat gatgaatatt gcgaagtaac
    51721 accagaaagc attcgcttga gaaaacaaat tttagagaaa aatgctcgtg aaaaagcatc
    51781 taaaaagaaa aaataatctg taaaaggatg aagagagaaa ccagcttgtt ataaaaaata
    51841 gctggtttct ttttttgtcc aactcttttc tgtggagaag tcatctttgt caatggacat
    51901 aaattcagaa aatacgccga aagacgttta aaagactttt aaaaaactta tttctagaga
    51961 gtttttgttt cataataaga taatgtttca cgaaaggaga tcaacttgtc cttcgaagaa
    52021 aatcgaagta aaatggcaag agaaaggagc gggaagatga acgacttatt aaaacaatac
    52081 tttggttatg atgagtttag acctggtcaa aaagaaatta tccagaaagt cattgatcag
    52141 gaaaatgtat taggaatcat gcctaccgga agtgggaaat caatctgtta ccagcttcca
    52201 gctttactat tagatgggct tactgtggtt gtgtcccctt taatctcttt gatgaaggat
    52261 caggtggacg cggcgaatca attaggtata cccgccactt ttatcaatag ctcgttagat
    52321 ggttacgaaa cagctagacg cttccaagaa atcgatagac agcagtaccg tttactttat
    52381 atcgctcctg aacgttttat catgccggat tttatccaag caatgaagcg atggaacgtc
    52441 cgtatgatcg ctattgacga ggcacattgt atctctcagt gggggcatga tttccgccca
    52501 agttatttac agatggcgaa ccagctagat caactgccaa atcgtcctgt aattgttgct
    52561 ttgacagcta cagcgactgt gcaagtagct gctgacataa aaagactttt aaaaattcca
    52621 gaaaacaacc atattcagac tggttttgaa cgagaaaatc tacgatttca agtaattaaa
    52681 gatcaaaaaa aagaacaata tctaatcgaa tatttaaaga tcaacaaaaa tcaatctggt
    52741 atcatttatg cagcaacgag aaaagaagta gaccgtctct atcatttgct taaaaaattc
    52801 gatttttccg tgggtagata tcatggtggt ctaaatgaga atgagcgaac agaaatgcaa
    52861 gaagcctttt tatacgatcg cttgcagctg attgtagcga caaatgcatt tggtatgggt
    52921 ataaataaaa gcaatgtccg ttttgttatc cattatcaaa ttcctggctc tctagaagca
    52981 tactatcagg aagctggtcg ggcgggaaga gatggtcttt ccagtgaagc catcctgctt
    53041 ttctctcctc aagatatcca agtccaaaag ttttttatcc agcagtccca aagagaagaa
    53101 gggcaaaagc aaaaagaata tgaaaaatta aaagcgatga cagaatacgt gtatattgag
    53161 tcctgtcttc agcaatacat cctaaactat tttggcgaga caagcagtcc gtgcaaccgt
    53221 tgtggtaact gtttagatga cagagaaata gtagaagtaa cgacagaagc acagatggtt
    53281 ctttcttgct taaaacgtat gggtgagaac tatggtaaac aaatgctgat gaaagtctta
    53341 gcaggatcga aagaacaaaa gctgcaagct ttaggttttg gacatttatc tacctatgga
    53401 ctgatgaaaa accaatcgca aaaagaaacg atgcagttaa ttgaatattt gatatcgaat
    53461 ggctatcttc tgaccatcaa tggtgagtat cctgttttga aagtaacaga aagagggata
    53521 caagtattga aaggacaaga atctgtttat cgaaaggaac ccaagaaagt ccagcagcta
    53581 tcggatgaag aaaccgatac actatttgaa gtgctgcggg aactgcgaac ggatctagca
    53641 tcagaagcag gcgttccccc gtatgtcgta ttttcagatt ccacattgaa agaaatgagc
    53701 cgaatacgtc catcaagcag attggagatg cttcagataa aaggagttgg ccaaagcaag
    53761 cttgataaat atggcgaagc ctttttatct cggataaaaa atgcggactc taacgttctg
    53821 gataagtaga aagaacgaat acgtaaaaat gaataaaaac aactggttaa atctgttttt
    53881 tttgctcctt atgaaaatca aatatgttat aattaaggaa ttagaatggg aggtaagcac
    53941 agatggattc tttgtcgaaa aaacttgcga tccaagtatt aaatgatgaa gcagaacgga
    54001 tcaaacgatt aatccgaaat caaaacaaca gtctctgtat ctcacaatgc aaagccttcg
    54061 aagaagtggt agatacacag atgtacggct tttcccggca agtcggtttt gctgtccgta
    54121 taggagtaat cggaaagaac gagggccagc tgatgttaag tgagctagaa cgagaattga
    54181 atcagttata tagtgaggtt taccaagaaa tttatgataa aaaagaagtt ggcaaggagg
    54241 aatagacttg cctaagaaag tgaaaaaaag gcatctatta gactatagca tcttgattcc
    54301 ctatttaatt ttatgtgtat tgggattgat catggtatat agttctacgt cgtatctatt
    54361 attagaaaac ggacaaaatc cgtccgcatc agtcattaac cagagtattt tttgggtgct
    54421 tagcttgatt gctatcgcac tactttataa aatgaaaaca gatgttttga aaaaccagcg
    54481 tttgatcatg gcagccatcg cagttttgac gattttgctg ctgattgttg tgttttttgg
    54541 taaggagatc aatggggcga aaggttggct gcaaattgca ggattctcga tccaacctgc
    54601 cgaataccta aaaatcattt ctatctggta cctgtctctg actttatcta aaagacaaaa
    54661 cagtgtacaa aaagattttc ttgggactgt gaaacgtcca cttgcaatgg ttatcggact
    54721 tactgcatta gtcgctattc ttcccgattt tggtaatgcg gcagttatct ttttgattat
    54781 tttggttctg cttttagcca gcggagtcaa ttatgtgtat actttgattg taggagtagg
    54841 aggcttttgt ctaagtactt ttacgatctg gctgatcaat atcacaaacg gaaagatctt
    54901 acccggacgt ttacagtata tctataatcg atttgctatt taccaaaatc ctttctcaga
    54961 cgaattaaat aaagggcatc aactagtcaa cggctactat gccatgttca acggaggact
    55021 atttggcagg ggattaggta acagtatcca gaaaaaagga tttttacaag aagcacaaac
    55081 cgattttatt tatgcaatcg tagtggaaga actgggagtg attatgggga ttttgatatt
    55141 agcgttgcta ttctttatga ttgtgcgcat tgtccttgta ggtatccgtt caaaagatcc
    55201 gttcaattcg cttttgtgca ttgggatcgg cgcaatgttt ttgatccaag tatttgtcaa
    55261 tcttggagga attactggcg ttattccttt gacagggatc acgttcccat ttttaagtca
    55321 aggaggctcc agtttgctga tgctttcgat ttgtgtcggc tttgtcctca acatcagtgc
    55381 ggatgaaaaa cgcaagagct taggtttata ataaaaaaag atgtaggagt ggttcgatga
    55441 aaaaagtttt agttgctaac aggggtgaaa tcgctgtacg tgtatttcgt gcttgtacag
    55501 aattaggaat taaaactgtt ggaatctatg cagaagaaga tgaatattcc gtacaccgtt
    55561 tcaaagcgga tgaagcatat ttagtaggac aaggaaaaaa accgattgat gcgtatctag
    55621 atattgaagg gatcatttct atcgcgaaag aatgcggagc tgacgctatt catccgggtt
    55681 acggtctttt gtcggaaaac ttgaattttg cgcaacggtg cgaagaagaa ggcatcattt
    55741 ttgttggacc caaactgcat catttagata tttttggaga taaaatcaaa gcaaaagctg
    55801 cagctatcga agcggggatt gcttcgattc caggcacaga tggaccaatc gcttctatag
    55861 acgatgcctt ggagtttgcg aaacaatacg gttatccgat tatgatcaag gcagctctag
    55921 gcggcggcgg acgcggcatg cgtgtagctc acgatgaaaa gagtgcaaga gagggctacg
    55981 aacgtgcgaa aagcgaagca aaagcagcct ttggtagtga tgaagtgtat gttgaaaaat
    56041 acatcgctaa tcctaagcat atcgaagtac agattctcgg ggatacgcat ggaaacgtca
    56101 tccatctttt tgaaagagat tgttctgttc agcgccgtca ccaaaaagta gtagaagtag
    56161 cgccttgtgt ctctatgaat gaacagcaac gtcaaaaaat ctgccaggca gctgtccaat
    56221 tgatgaaata tgtcgggtat gtcaacgcag gtacagtaga atttcttgtt gaaggagatg
    56281 atttttactt tatcgaagtc aaccctcgcg tccaagtgga acatacgatc acagaaatga
    56341 ttacagatat tgatatcgta acaacgcaac tgttgattgc tcaaggtttg gatctgcata
    56401 aagaaatcgg attgcctcag caagaaggaa ttaaattaaa tggttcagcc attcaatgcc
    56461 gaatcactac agaagacccg ttgaataact ttttaccaga cacagggaaa atcgatacgt
    56521 atcgttcacc tggtggtttt ggcgtacgtc tggatgtcgg aaatgcttac gcgggctatg
    56581 tagtgacacc gtattttgat tcattgctag taaaagtatg tacgcatggt gctacctttg
    56641 aaacagccat tcagaaaatg gaacgttgct tgaaagaatt tagaatccgt ggagtgaaaa
    56701 caaacattcc atttatgctc aatgtcatca cacatcctga attccaatca ggaaatgcaa
    56761 aaacaacctt cattgatagt acagcaacat tatttgaatt tcctcgacta cgagaccgcg
    56821 gaaacaaaac gatgaaatat atcggtgaaa ttactgtgaa tggttttcca ggtatcgaaa
    56881 gtggcgaaaa accattttat gaagaaccac gtatgcctaa agatctaatc acaagaactg
    56941 attacgtaac ggctaagaat gtattagatg cgaagggtgc cgatgctttg gtcgaatgga
    57001 tcaaaggaca ggaaaatttg ttgttgacgg atacgacatt tagagacgcc catcaaagtc
    57061 tgctagcaac tcgtgttcgg acaaaggatt tcaagcagat tgcacgtttg actggagaag
    57121 gtcttcctga actcttttca agtgagatgt gggggggagc aacatttgat gtggcttacc
    57181 gctttttgaa tgaagatcct tggcaacggt tgagaaaaat tcgttcttta atgcctaata
    57241 cgttgctgca aatgcttttc agaggttcaa atgccgttgg gtattctaat tatcctgaca
    57301 atgtattggt ggaatttgtc aaagaagcag cagcgcaagg aatcgatgta tttcgaatct
    57361 ttgatagttt gaactggact ccacaaatgg aaaaaagtat ccaagctgta cgggatactg
    57421 gtaaaatcgc agaggcagct atttgctata ctggcgatat caacgatcca agtcgggcaa
    57481 aatacaatgt tcagtattat aaagacatgg caaaggaact agaacaactg ggtgcgcata
    57541 tcattgcaat caaagatatg gctgggctgc ttaaaccgca ggcagcttat cgtttgatta
    57601 gtgaattgaa agaaacgaca gatctgccaa tccatctgca tactcatgat accagcggga
    57661 atggaatcat cacttattct gctgcttcaa aagcaggagt agacatcgtt gacgtggcaa
    57721 tgagtgcaat gagtgggaat actagtcagc ctagcatgag cagcttgtat tatgcactgg
    57781 tcaatggtcc acggctgcca gaaatcacga ttgaaaatgc acagaaatta aaccattatt
    57841 gggaagatgt gcgcatgtat tacaaaccgt ttgaaaacgg cttgaatgct cctgaaacag
    57901 aagtatatat gcatgaaatg ccaggaggac agtattcgaa tctacagcaa caggcaaaag
    57961 ctgtcggatt aggtcatcgc tgggatgaaa tcaaacaaat gtatcatact gtcaacttga
    58021 tgtttggcga tatcgttaaa gtaactccat catctaaagt agttggagat atggcactat
    58081 ttatggtcca aaatgatctt acagaagaag atatttatga aaaaggcgaa acactaagtt
    58141 tccctgaatc tgttgtgact ttcttccaag gagaactagg gcaacccgtt ggcggatttc
    58201 ctaaaaaatt gcaaaagatt attcttaaag gcagacctgc attgaatgaa cgtccaggtc
    58261 ttttggctga atcagtcgac ttcaacgaag taaaaaaaga attggcagaa aaaattggct
    58321 atgaaccaaa acaagacgaa gtattaagct atttgatgta tccgcaagtc tttttggact
    58381 atcaaaaagc atataatcaa tttggcgatg ttacattact ggacacacca acatttttcc
    58441 aaggtatacg tttgggtgaa acaatcaatg tacaaatcga acgcggaaaa acgcttatca
    58501 ttcgtttaga tgaaatcgga gaacccgata tcgaagggaa tcgcgtactg ttcttcaatt
    58561 tgaatggaca aagacgtgaa atcactatca aagataattc gatcatcagt acggtccaaa
    58621 cccgattgaa agctgaaccg acaaaccgag aacaaattgg agcaacgatg tctggttctg
    58681 ttttggatgt attagtgaaa aaaggcgata acgttaaaaa aggtgatacg ttgatgatta
    58741 ctgaagcgat gaaaatggaa acggccatcg aagcacgttt tgacggagaa gtcgcacatg
    58801 tatatgtgtc ttcaggagac actatttcgt ctggtgacct tttgattgaa gtgactgaga
    58861 aataataagc gaaaggtggg gaaacatgaa aagactgggt ttgttttggc tgattctgct
    58921 attagttatt gtggcattct acatggaacc agtcttgttt ccgccaaaac ttgaagtgaa
    58981 ggaaaactct gatcaacaaa tatcaaacca aatgacaacg tggaaatacc aggagcttaa
    59041 agcaactggt ttttctactt atatcggaaa accgatcgat cagctggaaa aggattttgg
    59101 ccaagcctat gatcgattaa ctagcggatt tggttttgaa acaagatatt atgaaaataa
    59161 agctagtcat ctcacatttg aagcgaacat ccaagacagc agagtcacaa ctgttaaagt
    59221 actgcaaaca gggcctgctg atatcgcgcc gttttattta gggatgacga tgcaagatct
    59281 tacgaaaatc actactatct atactaattt cacttttgag taccatgata cagaagttgg
    59341 aatcgaacta atggaagagg atatgaatta tcgtccgcta attgcttttg ataacgacac
    59401 ttttgctatt ttattttttg atcaaacaac aaacggccta ttttcagttg tgtatttaga
    59461 taaagatagt cttttgaaac tattgccata tcaagtgttt ggtgaaggcc ttccacatta
    59521 tcaagcagat aaatcggctg attgggaatc aatcaatcgc gttaaagaac gaaaaagtac
    59581 gactttatta aatatgctta gaagagaaga cgaacttccc atatacaatc aaacacttgc
    59641 ctttcaaaac cacagtcagc tgctactaca tgattttttg acaaggcctg aggaaatatt
    59701 gtcagaggaa cgtatggctg attggcaaaa atcgctaggc agtgctcaaa cgacagtgaa
    59761 attctcactg acgaatgatg aattggagaa gttaatcagt aaacaaaaat tagaaaatac
    59821 tagtgggcta tttattcatc cggcgattga cgctactttt tcgtttttat attggtacag
    59881 cgatccttac aaccatgacc gtttcatgga cccgggaacg gattctgtag gcattgcttt
    59941 ttctaaagaa aatatgttag tcttattgca agaagaaaac ggagaaagta gtgatagcag
    60001 tgatcgttaa cgaagagctg tttcaactgg aggatcaatg cctccaagtc gctgaaatga
    60061 taaaaaagag taaaacgatg gcttcttatc ttgaacataa acagtcaatg gaagaatctg
    60121 caaaagtttg ccagttgaaa gaagcgttct tgtctaagaa agaagcattt gaaaatgtag
    60181 cggcatatgg tgagtatgca cctgattatc gtgaaaaaaa acgagctgtt cgaaaagcaa
    60241 aaagacagct tgatatggat gagcatgttg cagctttccg atatagtgaa acacagctgc
    60301 aaagtctgtt ggatcaaatt gtccaagtac ttgccgaaga agtttctaat gaaatcaaag
    60361 tagatgcagg tagcccgttt tttgaaacga aaaaacactt tggatgcgga ggtaattgtc
    60421 atgcaattag acaatagtga atttgaaata acaaaacgac gcgggctgat cgtatgggtc
    60481 tacagcttaa aacaattaaa gaatctgaaa aagttcggct tgttgcatta tgtctcaaga
    60541 agaatgaaat atgtcgtgct ttacttaaat gaagagacat tggaagaaac agaagaacga
    60601 atccgtaaat tacattttgt tcgaaacgta gaacgttctt accgaccgga tatcgaaatg
    60661 aattttgccg aaaaaatagg aaacaaaaca gtattaaaag acgaaggttt cgaaattgaa
    60721 gagctaaata cagaaatccg attagctgaa catctttaaa aatctcttat gagcagtttt
    60781 taaataaaaa ataacttgga taacgatcga tttttgatca ttgtccaagt atttttttgg
    60841 tatactagta ccagtaccat ttctaaggag tgagtagatg cggataatat caggaaatta
    60901 tggcgggcgc cgtttaaaaa gtttagcagg ggcgaacacc agaccaacta cagataaagt
    60961 aaaagaatcc atattcaata tgattggacc atattttgat ggagagacag tattggatct
    61021 gtttgcagga agcggtggtt tagctatcga agcagtatct agaggctgca gtcatgcggt
    61081 atgcgtagac aaaaattacc aagcgctaaa aatcatcaaa gaaaacatcg aaattacgaa
    61141 agaaccagag aaatttacca caataaaact tgatgcggac aaagcaatcg aacggctagc
    61201 agcggagaaa caacaattcg actacttgtt tcttgatccg ccatatgcaa aacaaaaaat
    61261 aatcgatcaa atccataaaa tggaagaact gggacttttt tctaaaaccg cagttatcgt
    61321 atgcgaaacg gataaaacag tcgaattgcc agaaactgtg gctggattcc atcaaataag
    61381 aaaacagatt tatggcataa cggccgtaac tatctataga aaagagggaa atcaatgaaa
    61441 cgtgcattat tcccaggaag ttttgatcct tttacaaaag gtcacttaga tacggtggaa
    61501 cgtgcggcta aattattcga tgaggtagta attggtgttt ttatcaatac ttcaaagaaa
    61561 agtttgtttc ccccagaaga aagaatgaca ttgatcacaa aagctgtttc tcatcttcct
    61621 aatgtaaaag tcatgcatca agaaaatcaa ttgacggtag aaacagcaaa agagattggt
    61681 gcagatgcgt tgatcagagg catacgaagt atcaaagact ttgaatatga acgagagatt
    61741 gctcagatga atcatcatct atatgatgaa ttggaaactg tcttcttatt agcaaaacca
    61801 gaatatagtc acgtgagttc cagtatttta aaagaagtac tgcactttgg cggagatgtg
    61861 agcagctact taccgccagt gatcaacgaa gctttagctg gaaagcgtga atcaaatgaa
    61921 agctaaacaa tggttgaaaa cagtactgat tatcatattt ggaatgttga tcattggatt
    61981 tgtagtagtt cccttgccgt attatgtaga atcaccggga gccacgatcg atctgaaaga
    62041 actaattacg gtcaatgata aggaagataa aggttctggg tctttctctc ttacctctgt
    62101 tggtatcagg caagctacag gtttcttagc tttaaaagct aaaatgaccc cttttgaaga
    62161 attgatcagt gaagaagaat tgactggtgg ggcgacaaat gaagaatata atcaaatgca
    62221 acagtattat atggattctt cccaaaatgc agcaatcgaa caagcgttaa aacttgcaga
    62281 caagccttat aaaatggaat tcaaaggcgt atatgtcatg agtgtagaga aaacatcgaa
    62341 tttctttggc aagctttcag ttggagatac agtaacaaaa gtagacggga aaagctttca
    62401 aagtacagaa gcatttatgg attatgtaaa aagccaaaaa gttggacaaa caatcgaaat
    62461 cagttatttg cataatggtg aagaaaaaac agcttctggt gatctgatcg agttaccaac
    62521 agataaaaag gcaggaatag gtatcacgct tactgaccat accgagatcg aatcagatac
    62581 agatgttcgt attaattctg gctctatcgg tgggccatca gcgggattga tgttcacttt
    62641 agaaatctat gaacaagtca ctggtaaaaa cttgcgacat ggtaagcaaa ttgcaggaac
    62701 tggaacgatc aacagtgaag gagaagttgg ccgaatcgga ggaatcgata aaaaagtggc
    62761 aagtgcggat aaagctggcg tagagatttt ctttgcacca gatgatacga tttcagacga
    62821 tgttaagaaa gaatatcctg aaataaaaac caactacgaa gaagcaaaag ctgccgctaa
    62881 aaaattgaat tcctcaatga aaatcgtacc agtaaaaaca gtacaagaag cgcttgacta
    62941 tctagagaaa atgaaatgaa actaaataat taaagagggg ttgtgacacg agttctgtca
    63001 caacccctct ttttcgtcaa aaatcttcac gataaaaata aaaacaaaag aaaaaattga
    63061 cgtaattaga tgttgaaggg acgattttgc cagagctcac tgtttcttca attttaaatg
    63121 aaaggagtaa tgaatgattt ttactggtca ttctttgaaa aaagaacaat gatagaaaaa
    63181 atcagcaaga aaatactagc ttcacccaaa ttgatcattt ggatgtctat gatagttctt
    63241 atcttttgta tcgcaagtgg ttttttatta tcaaatttgc tattttatag agaaacagaa
    63301 gagactgctg caaaagaaat actttcttcg tttagttcca gtggagaaga tccatcagct
    63361 gaaggaaacg atgtagaaga ggatgctcgt ccaaaggtca tgtacaccga tatcaaagga
    63421 agtgtgaagg agcctggtat ctattccttt tcttcagaag aacgggtcta tgatgtacta
    63481 aagcgtgcag gtggattatt agaagaagcg gatagcgatc gaatcaactt ttctgcgaag
    63541 atagaagatc agcaagtcct atatatccct gctgtaggtg aagagctgcc agaacatctt
    63601 gatcagttgg catcctcgga aggaaaacag cctacagcag attccgagcc tcccaaaata
    63661 aatatcaata cagccactcc atcagaactt cagcagattc ctgggatcgg atcagtcaaa
    63721 gcgcaagaaa tcattcgatt tagagaagaa aacggatcct tccagaaagt ggaagacctg
    63781 caggagattt ctggtatcgg tgaaaagaca gttgaaaaac taaaaaactt cgttacaatt
    63841 aaatagaatg attcaaaatc gactggcacg agcaataaat ggacgctaat ggatactaag
    63901 aataatccaa ttctaagagc ttggcaggac gaggaggtag tatttttgga taggaaatag
    63961 cttgattgtt cgcaaagaaa cattaaaaaa accaatgctg aatgtctatc tccaaaataa
    64021 aatatctggg attcatatta tgaatacggc tgtcagcggc aataacagtc aagctctacg
    64081 agaacgcttt gccaaagatg tacttagcta tacagcagat aaagtattta ttctcattgg
    64141 gacgaatgat ctagcagagc ataaacaact ttctaaagaa acgtaccaaa aaatctgcag
    64201 tggatgatag ttcagctgaa aaaagactat agttctgatc atgctttctt tatcacgcca
    64261 ccagcagtcg atgagacaaa acaaaaatat cggaataaca agttgatcaa agaatatatc
    64321 tctgttctca cagaagtagc aaaacgccat gattgtgaag tatcgatttt tatacggaat
    64381 ttcttgaaca aatggattcg aagaattttt ataatgggct aatggatgac ggacttcatt
    64441 tcggcagtga agggtaggat cttttgtcag acaggatgat tcgttatatt taaagaatat
    64501 tttgtttaag caatgagtac tttttaatca acttgaaaaa acggaggaat ataaaacatg
    64561 ttagaaaaat cttcaaaagc atgcgatacg aataaagaaa aagttactga taagtcacat
    64621 aatgaacgca ttccttggga ccagtacttt atggctcagg cagtactgtt atctttacgt
    64681 agtacgtgta cacgtttaga agtaggagcg acactcgttc gagaaaaaag gatcattgct
    64741 ggcggctata atggagcagt cagtggagat gtccactgca tagatgaagg gtgttacgtc
    64801 gtggatggac actgcttgcg aacgatccat gcagagatga atgcactcct acagtgtgca
    64861 aaacttggta tcccgacgga aggttcagaa atctatgtca cacattttcc atgtttggcc
    64921 tgtacaaagg ctttgttgca agctggtgtg aaagggatca attatctcca tgactatcga
    64981 aatgatcctt atgcccaagc gttgatcgaa caattaggtg tatctgtaca ccaagttgct
    65041 ttagactcac agcatttttc aaaacttcaa aatgaattaa caggaaaaca tgtctagcgt
    65101 tttagaaagg catgttcgaa atcggttgat ttttccggca attacgtcga tgattatctg
    65161 tttgttgtat tacactgaca attggggtta tcgtttgctt cttgccggaa tttttttatg
    65221 gttttcgcta aaagaaggac gttttatcgc gtacttgtgt attttgtgta gcataacggt
    65281 atccatttct tataggctac aagagtattc atttttagag gaaaaaacaa catctgcgca
    65341 aattactgta tttacagata cgattcgtgc aaatggtaat tttttgacaa tggaaggaag
    65401 gttatcagag aataaacaaa agctccagct ttcttatcaa gtaaacacag aagcagaatt
    65461 gaaaacatgg cttaacttcc gaggaaaggg aatagaattg actgctgaag gcgagttcat
    65521 ctctccagca aaaaagcgga atctttataa ttttgaccaa gaagcttact tcaggaacca
    65581 tagaatttct ggacggttcc aaattcaaaa gattgaacag atcaccatgc aaaatcactg
    65641 gcgaaactgg cttcagagaa aaagaggatc ttttatctcc tttgtccaat ctgaatttcc
    65701 gcaaaaaaca agtgtatata ttaattcttt attatggggc tataaagatg caggattcag
    65761 agaaccagaa gaaattttta gggagagtgg tttgctgcat ctgttcagtc tatcgggtct
    65821 acacatccaa ttttatttag ggtggctgta ttatttattt cgacgtatcg gatggccact
    65881 acacgtcagt attattccgt tgagtctact aactatctgt tatgtttcta ttgcaggaag
    65941 ttctatttct gttttgcgtg ctagtttgct gtttttattg cgattgctag tcaaattgct
    66001 gaataagcat tattccacca tggacttatt tgcagttgtc ttttggcttc ttttactatt
    66061 tgatcctcga tttctttttc atgcgggtgg acagcttagt tttttcatga gttttttgtt
    66121 tatcctgatt tcttttgatg gagattggtt gaaaaaaacg gcctctcacg tcctgtttac
    66181 tatgatcacg atgccgttga ttgtatggca tttttatgag tggcctattt taggtagtct
    66241 ttttacatta ctgattgcac cggtattcaa gtttcttttt cttcctggtg tgttatttct
    66301 cacactcttc aatcgctggc tgccggaaac tttacttttg ctgatagaag agggattaat
    66361 cttatttgag cgaataatcg atttttcttc agggtcgtct ctcattatag gacgtttgcc
    66421 ccttgtgcta tctgggatgt atattttcgg attactgatt tgtatggata ggttgaaaga
    66481 acaacctgtg aaacacttgt tttatgttgg tcttttttct tttatttttt tgactttgcc
    66541 atttttacgc ttttcttcaa cgatcgcttt tatcgatgtc ggtcaaggag acagtatctt
    66601 tttgcaaagt ccttttcgaa aagaaacgat tttgatcgat actggaggtc agttacactt
    66661 tgaaagagaa aagtgggcgc aaggtatgat acgtcctcct gctgaaagaa atatcgttcc
    66721 atatctaaaa ggtcagggga taagtgtaat cgataaactc atcttgacac acgacgatac
    66781 ggatcatgtg ggagaattac ccacgttatc tcagcatttt actatcaaaa aaatttatat
    66841 cggttggggc gctggacgaa atgagccatt acaaaggcat ttatctgaag ctcaaaaaaa
    66901 tggtactgag atcattgaaa tcaaacatgg ggacaggatc agcggttatt atgatcttta
    66961 tgtattgact ccttttgaaa agggagaggg aagaaatgaa gattcgattg gtctatggat
    67021 ggaatataac aataggcgct ttttattttt aggagatttg aatcaagcaa tggaaaaaca
    67081 attgcttttg atttatccta atctgaaagc cgatgtagtc aaattaggtc atcacggtag
    67141 ccgtacgtca agtaacacag attttctttc ccatattgaa gcaaaacatg ggattatatc
    67201 ctgcggcgta aataatcgtt atggtcaccc gcacagcgaa gtattggaga cactagaaga
    67261 aaatcagata aaaacatggc gaacggatca acaaggaatg atcacctatc gctggcatcc
    67321 agtttttcat cctcatgggc tagtggaaac aatgatagac tagtaattct catctgctca
    67381 tggtaggata atttttgaaa gtggtgagta atttgagtat acaagcagag ttacaaaaaa
    67441 tacgtaaaga aaaacttgca tcttgttact tgattttagg aacagaaaag tttctccaag
    67501 atcaagttcg gacagaaatt gtcaaaaaaa tccaattaga gggcgaagat gacttaaatt
    67561 ttttaagttt tgacatggaa aatgcaagtt tagatgaggt agtagctgaa gcagagacgt
    67621 tgccgttttt cggagatcaa cggttagtat tcgtagagaa tccttatttt ttaacaggtg
    67681 aaaaagtaaa caatggaatc gaacaaaata ctgatttatt gacggcttat ttgaaagaac
    67741 cattagaatc gactgtatta gtattctttg cgccatatga aaagttggat gaacggaaaa
    67801 aagtaacgaa acaattgaaa aaaacagcaa ttatcatcaa tgtccaacag ttgaatgaaa
    67861 aagaagtacg tcaatatctg atgaatacct tagagaattc ctcaatcaaa atggaacgaa
    67921 gtgcaattga tttattcttg aggttgaccg acttagattt atccaaaatg atgagagagt
    67981 tacagaaatt ggtcctttat ggacaaaatc aacagcaaat cacggtaaga gaagtagaag
    68041 aattagttcc taaaacgtta gaacataata tttttgacat gacccaatat attttaaaag
    68101 ggaagacgga acaagctttg cgtttatacg aagatttagt tatgcaaggg gaagaaacaa
    68161 tcaaaattaa tgcgattttg ctttctcaat tacgtttatt tttacagaca aaatttcttg
    68221 taaagattgg ttatcagcag gcaaatattg ctgaaacatt gaaaatccat ccatatcgtg
    68281 taaaacttgc catgcaggaa gtaaaaaaat tcgatgaaca tttgctggta cagttgtttg
    68341 atcaattagt tgagatggat tatcaaatca aaacgggaca aatcgaaaaa gaattaagtt
    68401 ttcaactatt cgttttgcgc acagggcagt tgttaagaaa tcgataagaa gaaagacgag
    68461 ggcaaagcat gcgggaaggt cgtttgtttt tgtccttttt ttgacaaata aaaaaatcgg
    68521 ccgaagccga ttcttttatt tagcgatttt tttgcttaaa cgagatttgt cgcggttagc
    68581 tttgtttttg tggattagac cttttgattc agccatatca atagctttaa cagcttcttt
    68641 atataacgca tctacgttgt cagcacctga agctacagca tcttcaaatt ttttgattgc
    68701 agtacgcata gcgcttgttt gagatgaatt tttcacatta gcgttttcgc ttgtacgaac
    68761 acgtttgatt gcagattcaa tgtttggcat tagtttcacc tccgataact taagttctta
    68821 cgcgtgttac cgcataattc aacattcatt attataccta aaggcagcgt gctttgcaat
    68881 atgaacatga caagtttttt tccttaacag tcttttatta ggattttttt tcagtaaaaa
    68941 cggatagtac aagctgaaaa aaatcaaaaa gacgctattg tgggaggcaa tagcgtcaaa
    69001 ccaaactaga tttttattta tctatttaaa caaagtttga atgggaatgt ttggtactaa
    69061 gttattatat aacaaagaga accgtttgca aagttttttt tgaaaaggga tacgattccc
    69121 tgttgttctt tgtttttcta ttaagaaact gcagttattt tataaccagt atcctttgat
    69181 cagtaaaggc tattctggtg taccgattat tttaatgtat tcatcgatat tttttatcaa
    69241 catttttttt atgaccaact ggttccgacg gttccatttg ccacttactt ccagcacata
    69301 tttatttagg ggcaaacgta agatatattt tgccaaatag cgatctttaa gcaggccatt
    69361 gatcacacgg tcctcaagct gcaagttgat cccagtcagc atcattggct gttcttgtaa
    69421 aacgtacgta tcaattaaaa gaccttcaaa agtttccaca agcgtcttcc tttcttttca
    69481 aagtaacctg aaaacaggca ctaattcttc cattttgata ttcgttgaag tggatgcgat
    69541 cattctttct tttgtccact gtaatctagt ataacaaaaa agagcagaag agccaaaaag
    69601 aaaaaagagc atcgaattct ataggaagtc tcattggcac ggcctaaatt attacaaaac
    69661 attgattaaa aaaagcccct gcatcgcagg agcatcccct atatcgtttt aataggtcaa
    69721 tcaaaaagca gtatcaaaca cattttgtat caaatcagga aataatagat aagctaagcc
    69781 cagaatcaaa agtaccgcta gaagaaacca gccgattgtt cctatgaaat actttaattt
    69841 ttcgaacatt gtatcacctc gttgtcagta tatcacggat cagtaaattg ggggtgttaa
    69901 gaatctccta aaatagacaa aatttgttct attttcctaa agcaaatact tgtaagatac
    69961 agtaagtgat accagaagca atgaccggtc cggcggcgat tcctcttaag aagacaacac
    70021 ctaaaatcgt gccgaaaaca agtgcaacag tgatttgtgg atcagaagca atcaatccta
    70081 cacccttgga agataagaca gccactagcc caccgcaaaa aacagctacc cagccaatag
    70141 gggatttaaa ggcttctaat agatcacgta gaccgatttc tcctgttgcg atagggatta
    70201 gaatagccgc tgtgatgatc gtcaccccaa tattgattcc tttgccttga agccaaggga
    70261 aaaaggaacg ggaggctggt atgagcttca taatcaaaac aaaagctgcc gctatcatca
    70321 gactttgatt tttagctaaa attgctacaa gtaaaataat tcctaaaaaa agccaacttt
    70381 ccatgaaata tattcctcac tttcttcgaa acaatcataa catgttttta gaaagttaca
    70441 aaagtaggaa ttcatccttc aagacctatt cataagctgt ttttttcata aaataaacaa
    70501 aatcataaaa aaacactttg cttatttgac agaagacagc cgcgctttgt atagtggaca
    70561 agacttacat ttttttgaag aatcagaaag atcaggtaaa ggtcttagta aacgcaagcc
    70621 attcagaata accaatattg tgcttccttc gtgtcctaca actccaagag gtagatttaa
    70681 aaattgcagg aagttagaag caatcaacaa gagaataaca agaatcgaaa aaataatatt
    70741 ttgtttgatg atccgccgca tttttaaaga tagccgatga ctgtagacaa atttatttaa
    70801 gtcgcttttc attaagacga tgtctgcgat atccatagcg gcatctgtcc cttgccccat
    70861 agcgacacct aaagaagcgg ctgctaaagc aggcgcatca ttgactccat caccaaccat
    70921 cgcatttact tggtaaagtt cattctcttt tttgactagt tgtgtttttt cttccggagt
    70981 acagcttgca tagtaatgtt caatccctac ttgttcagca atggcctttg ctgtgccaga
    71041 atggtcgccg gttatcatac ttgtatggat gttttgggat ttaaagtagg aaattacttg
    71101 ttgggtattt gcttttggaa tatctagcag acctaaaaca gcaattaact gctgatcttt
    71161 agaaagataa atcacggttt tgccttgatt ttccaaacgt tcgatcattt ccgcaatctc
    71221 tttgctgatt atcatcgtct caggatcata tgcgtgcttg ccaactttcc attgcgcacc
    71281 taaataggta gtttgtaatc caaaaccagt aatttcttct attggtaagt attggatttc
    71341 ttcaggtatt gtgcaatcga aatattgcaa aatcgcatga gcaagtggat gcgtcgtttt
    71401 ttgttccata gcaacaagaa gttgttgtgc caaatccttt tcatcaaaga aaaagctgtc
    71461 agttacaact ggttttcctt tagtgagcgt tcctgtttta tcaaaggcaa tcgcttttaa
    71521 ttcagaaagc ttttctaaat ggataccgcc tttgattaaa atcccatttt ttgcagaatt
    71581 tgaaagagca gctagagaag ctggtgtagc agatgcgaca agcgcacaag gagaagccac
    71641 aactaataat accattcctc gataaaagct ttcatcccaa ctccaaccta ggaaaaagtg
    71701 ggggagaagg atcattagag gaacagcaag caaaatcaat tttacataat tattttcgat
    71761 tcgttcgata aatgaagctg tttgagttgg cgtattctgc gcttcttcta ctaattggat
    71821 aatcttagca aagacagttt cggaactcgt ttttgttact cgaatcgtga tgggatttcc
    71881 ttgattcagc gttcctgcat acatctcatc gcctaaatat ttttctacag gcacagattc
    71941 accgcttaat gcagactcat cgatcgttgc ctgtgtgctt tctaaaaagc catctatcgg
    72001 aacagctgct cctttagcga caaataccaa gttatccacc tttagttgag aaacatctac
    72061 ttcttgcatt tgtccatttt cttttaataa ttgggctttt tgtggttgaa gattcatcaa
    72121 agcagtaatt tctttttgac ttttattagt tgtgtattcc tctaatgcac cgcttaaaca
    72181 aaagatgaat gtcaacattg ctccttcaaa gtaattaccg atcaaacagg caccaatagc
    72241 agctaaagcc atcaataaat ccacattcag tgtccgatca ttccacaatt ctttcaagcc
    72301 ttccgatgtt tgtttatatc caccgatgat aatggcactg atataaaaaa gtggggtgat
    72361 ggtaagagag aatcggtcga agataaaacc gaagatcata aataaaaggc agccgattgt
    72421 tgctgcaaaa gcttttttct gattagtcat cgttattaga cctccttgtc aaaacaagga
    72481 tagcataata aaaatgtaat tgataataat aacagctaaa tgagaacgat aatggttatc
    72541 atttggtgat taagcttgat gttgttttta ttatttataa tgattattat taaagaaaat
    72601 aaaaaaccag aacatttctc aaataagag
//
LOCUS       DAJSBN010000007        71080 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJSBN010000007 DAJSBN010000000
VERSION     DAJSBN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.007"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            161..391
                     /locus_tag="ONT86_000529"
     CDS             161..391
                     /locus_tag="ONT86_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCR2351348.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="ONT86_000530"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="ONT86_000530"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR2351349.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="ONT86_000531"
     CDS             1802..1978
                     /locus_tag="ONT86_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR2351350.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="ONT86_000532"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="ONT86_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR2351351.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="ONT86_000533"
     CDS             2593..3060
                     /locus_tag="ONT86_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2351352.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="ONT86_000534"
     CDS             3145..4029
                     /locus_tag="ONT86_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2351353.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4143..4379
                     /locus_tag="ONT86_000535"
     CDS             4143..4379
                     /locus_tag="ONT86_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCR2351354.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="ONT86_000536"
     tRNA            4467..4547
                     /locus_tag="ONT86_000536"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="ONT86_000537"
     tRNA            4554..4625
                     /locus_tag="ONT86_000537"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            4750..4920
                     /locus_tag="ONT86_000538"
     CDS             4750..4920
                     /locus_tag="ONT86_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351355.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5140..5847
                     /locus_tag="ONT86_000539"
     CDS             5140..5847
                     /locus_tag="ONT86_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2351356.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="ONT86_000540"
     CDS             complement(5907..6551)
                     /locus_tag="ONT86_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCR2351357.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="ONT86_000541"
     CDS             6678..6965
                     /locus_tag="ONT86_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351358.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="ONT86_000542"
     CDS             7073..7393
                     /locus_tag="ONT86_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2351359.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="ONT86_000543"
     CDS             7474..8787
                     /locus_tag="ONT86_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR2351360.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="ONT86_000544"
     CDS             8784..9503
                     /locus_tag="ONT86_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCR2351361.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="ONT86_000545"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="ONT86_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR2351362.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10404..12938
                     /gene="secA"
                     /locus_tag="ONT86_000546"
     CDS             10404..12938
                     /gene="secA"
                     /locus_tag="ONT86_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR2351363.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="ONT86_000547"
     CDS             join(13064..13135,13137..14165)
                     /gene="prfB"
                     /locus_tag="ONT86_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR2351364.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="ONT86_000548"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="ONT86_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR2351365.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="ONT86_000549"
     CDS             14940..15833
                     /locus_tag="ONT86_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2351366.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="ONT86_000550"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="ONT86_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR2351367.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="ONT86_000551"
     CDS             17770..18627
                     /locus_tag="ONT86_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR2351368.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="ONT86_000552"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="ONT86_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCR2351369.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="ONT86_000553"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="ONT86_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR2351370.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="ONT86_000554"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="ONT86_000554"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR2351371.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="ONT86_000555"
     CDS             21383..22141
                     /locus_tag="ONT86_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR2351372.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="ONT86_000556"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="ONT86_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR2351373.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23069..24100
                     /locus_tag="ONT86_000557"
     CDS             23069..24100
                     /locus_tag="ONT86_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR2351374.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24097..24864
                     /locus_tag="ONT86_000558"
     CDS             24097..24864
                     /locus_tag="ONT86_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR2351375.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24964..26532
                     /gene="liaX"
                     /locus_tag="ONT86_000559"
     CDS             24964..26532
                     /gene="liaX"
                     /locus_tag="ONT86_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCR2351376.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26829..27149
                     /locus_tag="ONT86_000560"
     CDS             26829..27149
                     /locus_tag="ONT86_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCR2351377.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27149..27499
                     /locus_tag="ONT86_000561"
     CDS             27149..27499
                     /locus_tag="ONT86_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR2351378.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27666..28604
                     /locus_tag="ONT86_000562"
     CDS             27666..28604
                     /locus_tag="ONT86_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCR2351379.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28619..29449
                     /locus_tag="ONT86_000563"
     CDS             28619..29449
                     /locus_tag="ONT86_000563"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR2351380.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29493..30518
                     /locus_tag="ONT86_000564"
     CDS             29493..30518
                     /locus_tag="ONT86_000564"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR2351381.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30533..31471
                     /gene="galU"
                     /locus_tag="ONT86_000565"
     CDS             30533..31471
                     /gene="galU"
                     /locus_tag="ONT86_000565"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR2351382.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31596..32036
                     /locus_tag="ONT86_000566"
     CDS             31596..32036
                     /locus_tag="ONT86_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCR2351383.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32039..32641
                     /locus_tag="ONT86_000567"
     CDS             32039..32641
                     /locus_tag="ONT86_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR2351384.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32700..33806)
                     /locus_tag="ONT86_000568"
     CDS             complement(32700..33806)
                     /locus_tag="ONT86_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR2351385.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34016..35017
                     /gene="ccpA"
                     /locus_tag="ONT86_000569"
     CDS             34016..35017
                     /gene="ccpA"
                     /locus_tag="ONT86_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCR2351386.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35354..37699)
                     /locus_tag="ONT86_000570"
     CDS             complement(35354..37699)
                     /locus_tag="ONT86_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR2351387.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37978..38880
                     /locus_tag="ONT86_000571"
     CDS             37978..38880
                     /locus_tag="ONT86_000571"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR2351388.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39093..40868
                     /locus_tag="ONT86_000572"
     CDS             39093..40868
                     /locus_tag="ONT86_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCR2351389.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40934..42520)
                     /locus_tag="ONT86_000573"
     CDS             complement(40934..42520)
                     /locus_tag="ONT86_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR2351390.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42771..42995
                     /locus_tag="ONT86_000574"
     CDS             42771..42995
                     /locus_tag="ONT86_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCR2351391.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43137..44888
                     /locus_tag="ONT86_000575"
     CDS             43137..44888
                     /locus_tag="ONT86_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2351392.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44888..46672
                     /locus_tag="ONT86_000576"
     CDS             44888..46672
                     /locus_tag="ONT86_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2351393.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46998..47849
                     /locus_tag="ONT86_000577"
     CDS             46998..47849
                     /locus_tag="ONT86_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2351394.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47903..49654
                     /locus_tag="ONT86_000578"
     CDS             47903..49654
                     /locus_tag="ONT86_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2351395.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49658..49828
                     /locus_tag="ONT86_000579"
     CDS             49658..49828
                     /locus_tag="ONT86_000579"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2351396.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49868..50770)
                     /locus_tag="ONT86_000580"
     CDS             complement(49868..50770)
                     /locus_tag="ONT86_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2351397.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51001..52404
                     /locus_tag="ONT86_000581"
     CDS             51001..52404
                     /locus_tag="ONT86_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR2351398.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52404..53837
                     /locus_tag="ONT86_000582"
     CDS             52404..53837
                     /locus_tag="ONT86_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR2351399.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            53982..54908
                     /gene="trxB"
                     /locus_tag="ONT86_000583"
     CDS             53982..54908
                     /gene="trxB"
                     /locus_tag="ONT86_000583"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR2351400.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55122..56849
                     /locus_tag="ONT86_000584"
     CDS             55122..56849
                     /locus_tag="ONT86_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCR2351401.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56864..57214
                     /locus_tag="ONT86_000585"
     CDS             56864..57214
                     /locus_tag="ONT86_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR2351402.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(57306..58166)
                     /locus_tag="ONT86_000586"
     CDS             complement(57306..58166)
                     /locus_tag="ONT86_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCR2351403.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58258..58686
                     /locus_tag="ONT86_000587"
     CDS             58258..58686
                     /locus_tag="ONT86_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR2351404.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58848..59024
                     /locus_tag="ONT86_000588"
     CDS             58848..59024
                     /locus_tag="ONT86_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR2351405.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59051..59497
                     /locus_tag="ONT86_000589"
     CDS             59051..59497
                     /locus_tag="ONT86_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCR2351406.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59651..61030)
                     /gene="brnQ"
                     /locus_tag="ONT86_000590"
     CDS             complement(59651..61030)
                     /gene="brnQ"
                     /locus_tag="ONT86_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR2351407.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            61736..62707
                     /locus_tag="ONT86_000591"
     CDS             61736..62707
                     /locus_tag="ONT86_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR2351408.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62731..64923
                     /locus_tag="ONT86_000592"
     CDS             62731..64923
                     /locus_tag="ONT86_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCR2351409.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64940..65416
                     /gene="ybeY"
                     /locus_tag="ONT86_000593"
     CDS             64940..65416
                     /gene="ybeY"
                     /locus_tag="ONT86_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR2351410.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65400..65795
                     /locus_tag="ONT86_000594"
     CDS             65400..65795
                     /locus_tag="ONT86_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCR2351411.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65810..66709
                     /gene="era"
                     /locus_tag="ONT86_000595"
     CDS             65810..66709
                     /gene="era"
                     /locus_tag="ONT86_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR2351412.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66852..67664
                     /gene="recO"
                     /locus_tag="ONT86_000596"
     CDS             66852..67664
                     /gene="recO"
                     /locus_tag="ONT86_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR2351413.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            68048..68965
                     /gene="glyQ"
                     /locus_tag="ONT86_000597"
     CDS             68048..68965
                     /gene="glyQ"
                     /locus_tag="ONT86_000597"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR2351414.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            68967..71042
                     /locus_tag="ONT86_000598"
     CDS             68967..71042
                     /locus_tag="ONT86_000598"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR2351415.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23266 a        12442 c        15206 g        20166 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtt
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10261 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10321 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10381 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10441 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10501 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10561 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10621 caaaacgtgt attggggtta tatccacacc acgtacaatt gatgggtgga atcgtgcttc
    10681 acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc
    10741 cagtatatct aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt
    10801 tagcaacccg tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg
    10861 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca
    10921 cttatagtac gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc
    10981 gccatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct
    11041 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt
    11101 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11161 atgtacaatc aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat
    11221 tcggtttgga taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag
    11281 cattgagagc caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag
    11341 tactgattgt tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac
    11401 ttcaccaagc aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg
    11461 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta
    11521 cagctaaaac agaagaagaa gaattcagag aaatttataa catccaagta taccagattc
    11581 cgacaaatcg tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa
    11641 gcaagttcca cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt
    11701 tagtcggaac tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11761 gtattcctca tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga
    11821 atgctggtca aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11881 tcaagttagg acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac
    11941 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    12001 gtatgtcaca gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac
    12061 gaatcaaatc tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca
    12121 agatgctgac gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca
    12181 cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata
    12241 aacagcgaca agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg
    12301 tcaaacggac aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga
    12361 atttggaaag tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc
    12421 ttgcagatat cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac
    12481 aagaagtatt taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg
    12541 aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg
    12601 atcaattacg tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat
    12661 atcaaacaga aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca
    12721 ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag
    12781 gaacagcttc tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa
    12841 aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga
    12901 aaaaatataa gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga
    12961 gacaaattct ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta
    13021 gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gccatgcaag aagaagcaga aacacgtatc aatgcgctag
    13381 atgaaaaaat gaagacatat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt tgaattgcat ccaggggctg gcggaacgga gtcccaagat tggggcagta
    13501 tgcttttacg tatgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggtgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa
    13801 gtggtgcagg tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc
    13861 ctacaggaac agtagtagct agtcaggcac aacgttcaca attgaaaaac cgtgagcaag
    13921 cgatggggat gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagcagttat ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac
    14161 aataagaacc cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcaattgt
    14341 catcgaacaa ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggacct
    14641 tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca
    14701 acgtgtctct attgctcggg caatagtgaa tacgcctaaa gtacttatcg cggatgaacc
    14761 gacaggaaat ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa
    14821 tgcccaagga actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 gcaccgagta attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagtt gcttcagtca gtgcggtaac gatcacattg actttagtcg
    15061 gtgtattcat ggcagttatc atgaacgcaa ctaaattagc tcaagatata gaaggaaacg
    15121 ttgatgtctc tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa
    15181 aagaattgaa tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat
    15241 tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc
    15301 cgttatatga tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag
    15361 aagaagcagc agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc
    15421 ggattttcca aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc
    15481 tgttcgtagc gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt
    15601 tccttgaagg tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat
    15661 ttggttatgc atggtttttc caattgatca atccatcatt attacgttcc cattattcat
    15721 tgattcatcc acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga
    15781 tcattggttc acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca
    15841 atataaataa aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag
    15901 gagaatagaa ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct
    16081 caaacatggg catgggtgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa
    16321 agtacgctct atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt
    16441 tatcgcagtg atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga
    16501 ggggtaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt
    16561 tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg
    16621 tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt
    16861 tttccgtcca ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg
    17041 gctcttcttt caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctaatcg agtatggagc aaggatcgaa
    17161 acatatgaga atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca
    17221 gttggctcag caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg
    17281 gtttatgatg aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt
    17341 tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa
    17401 ggattagcaa gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca
    17461 acagatggag atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
    17881 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
    18001 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
    18061 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
    18121 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
    18181 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
    18241 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
    18361 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
    18601 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
    18961 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
    19021 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
    19081 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
    19141 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
    19201 tttggcggga ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
    19261 ccgactgtca caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa
    19321 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
    19381 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
    19441 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct
    19501 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
    19741 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
    19801 aaccttccaa agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt
    19861 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
    19921 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
    19981 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
    20101 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
    20161 gattggcctt aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga
    20221 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
    20281 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
    20341 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
    20401 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
    20581 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct
    20641 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
    20701 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
    20761 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
    20821 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
    20881 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
    20941 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
    21061 cgggcaacag caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct
    21121 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac
    21301 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat
    21481 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
    21541 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
    21661 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
    21721 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
    21781 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
    21901 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
    22321 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
    22381 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
    22441 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
    22681 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
    22861 ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    22921 cagtcaaaca aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg
    22981 caagcttttg aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct
    23041 tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt
    23101 ttttatttgt gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc
    23161 tgtcatcgag atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact
    23221 gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca
    23281 aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga
    23341 atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat
    23401 tggcgccatg ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat
    23461 caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct
    23521 attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga
    23581 tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc
    23641 attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga
    23701 actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac
    23761 agctggagga acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa
    23821 gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga
    23881 tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc
    23941 gcttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc
    24001 attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc
    24061 ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt
    24121 gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta
    24181 ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg
    24241 ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt
    24301 atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt
    24361 tgtttcctac tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta
    24421 gaactagaag aattatctgg cttagataag actttagcga ttgattttag attacttacg
    24481 agatcgttgc ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct
    24541 agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat
    24601 tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca
    24661 ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag
    24721 caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg
    24781 tccgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag
    24841 aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa
    24901 tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt
    24961 tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa
    25021 gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa
    25081 gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat
    25141 aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga
    25201 caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact
    25261 tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag
    25321 aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa
    25381 gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat
    25441 acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa
    25501 tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat
    25561 caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta
    25621 ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa
    25681 tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt
    25741 tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg
    25801 ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa
    25861 atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat
    25921 catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct
    25981 aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca
    26041 ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta
    26101 actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac
    26161 atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa
    26221 aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg
    26281 aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc
    26341 ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac
    26401 gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca
    26461 gatggtgaaa tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat
    26521 acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt
    26581 gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga
    26641 acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt
    26701 cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa
    26761 gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga
    26821 ggtgagacat gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga
    26881 ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg
    26941 tattcctgag cttagttgct tttggaagtc caattatttt atacatttta ttagctttga
    27001 ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa
    27061 aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa
    27121 tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac
    27181 gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg
    27241 gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt
    27301 gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa
    27361 tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc
    27421 attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga
    27481 acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta
    27541 gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg
    27601 tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag
    27661 catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg
    27721 tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag
    27781 gactagagtt gactggttat tttaattatt attcccataa tcgtttacag ctatttggca
    27841 gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa
    27901 gattatgtag tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag
    27961 aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt
    28021 ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg
    28081 tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga
    28141 taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg
    28201 atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc
    28261 tggaacatct gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg
    28321 cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa
    28381 aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtgg
    28441 gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag
    28501 tagccgcgat gaatttccgc gcacgtacaa tgggctttga cgcaacgaaa acatttgaag
    28561 aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat
    28621 gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc
    28681 attgatcatc gtatcaggta tcattatcgc tatgtggctt agcagcagag aggctgttcg
    28741 ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat
    28801 tattggcgct cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat
    28861 ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc
    28921 gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat
    28981 cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt tcatgaacca
    29041 cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt
    29101 catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc
    29161 actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt
    29221 gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat
    29281 tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt
    29341 atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa
    29401 tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg
    29461 ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac
    29521 caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga
    29581 tttggggaca tcgtgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt
    29641 atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg gaagatgcag
    29701 taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa
    29761 aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag
    29821 aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca gcagaaaaaa
    29881 gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagca aaacatgaca
    29941 tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt
    30001 tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg
    30061 cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg
    30121 ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag
    30181 caatgggtgc caacccgctg acctttatcg gattaagcgg aataggcgat ctagtcgtta
    30241 cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc
    30301 ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag
    30361 cagcgatgga actagcccaa atgatggaaa tcgaaatgcc catcactcaa acaatctatg
    30421 aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg
    30481 gaaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt
    30541 taaaaaagca gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc
    30601 tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga
    30661 agcgttgaaa tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat
    30721 cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac
    30781 cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc
    30841 tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc
    30901 atttgtcgtt atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt
    30961 aatcgatgat tacgaacgta ctcatgcttc tacgatcgcc gttatgcaag ttcctcatga
    31021 tgaaacttca aaatacggaa ttatcaatcc aggagaagta ttggaagatg ggttatacaa
    31081 tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat
    31141 tggacgttac ttattgactc cagaaatttt tgatgtattg tccgagcaac aaccaggagc
    31201 cggaaatgaa atccaactaa ctgacgcaat cgatacattg aataaaacac aacgcgtatt
    31261 tgcccgtaaa ttcacaggta aacgttacga tgtcggtgac aaatttggtt ttatgaaaac
    31321 aagtattgaa tatggtctaa cacatccaga agtaggtgca cctttacgtg agtacatcct
    31381 gtctttaggt aaaacattag aagcagaagg aagcaagatc gatcataatc caaaaggaat
    31441 aacagaaatc aaagaagaaa agaaaaaata accctttttt ctaacgagct tcagctgcta
    31501 attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc
    31561 cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat
    31621 tgatcgcagc aatcgccttt gcgatacttg tcgtttttat cgttcgtgtg ttgttgcaag
    31681 tttctaaaac tttagaaaaa gtagatcaga ctgtagcaga agcaaatacg acgatcgaaa
    31741 tcgtcacaaa agacgtagat cttttgtcta gacaagtaga aggcttactg gtcaaaagta
    31801 atgaactgct cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag
    31861 ttgctgattt aagtgaaagt gtatcagaac ttaatcattc tagtaaaaac atcgcgacga
    31921 aagtcggcgg aatcggtaaa acgacagcta aagcgacggt tgccggaaaa gtcggtggga
    31981 cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga aaataaaaat
    32041 ggctaaaaaa ggtggatttt tattaggtgc agtagtcgga ggcacagccg cagcaatcgc
    32101 cgcgttattg ttagcaccaa aatctggtaa agaacttcgg gatgaattag ctgcccaagc
    32161 ggatgatttg atggatatgg cttcagatta cacagatatg gcgaaagaaa aatcgactga
    32221 gttaacaggt attgcaaaag aaaaagcgca agatctttct caacaagcag gtgatctagc
    32281 aggtagtgtc aaacaaaaag taagtgaatc agcagatgaa gcaggaagtg aatctgacga
    32341 tatcttgtta aatcttaaag aacaatcagc tgacttatct aatcgtttca aaaaaacggc
    32401 agaagatatg aaaaaacctg cagatgaatt aggaagtatt gctaaagata cttcagaaga
    32461 tatcatgatt gatgtaaaag acgctgctgg taaagcagga gatactgtca atgaaggaat
    32521 gtcagaagca aaaccagttg cagatgactc aaaagaaatg atgaaaatgg caaaagaaga
    32581 tgcgaaagaa gctaaaaaag aaataaaaga tacaatgaca gattcatcta caaatcacta
    32641 aaatcacaaa tttatagaat aaaaaggtgg gagcggacta gctctcacct tttttactct
    32701 tatttttcta aaacaagtaa ttcttttggt gtagtcgtga atggcacaca gccatcgctt
    32761 gtcacatata cacagtcttc gatacgtacc cctacttttt caggaatata gattcctggt
    32821 tcaagagaga agcacatccc ttcttcgatt actaagtcat ttccttctac aagggaaggg
    32881 aattcgtgga cggttgttcc gatcccgtga cctaaacggt gattgaaata ttcgccgtag
    32941 ccatagctgt tgatcacgtt tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga
    33001 cggacggctt cttgtgcggc aagctgtgct tcaagagtga tgttgtagat tttttcttgg
    33061 aagtcggttg gtttttgata agcaattgta cgagtagcat cactgcaata gccattccaa
    33121 acaacgccaa gatcgaataa cacaagttca ttcggtgaaa tcttcgtatt tcctggtgta
    33181 ccatgagggc ttgcagcatt tgctcctgca aggactaatg tgtcgaaaga catttgtgaa
    33241 acaccttgtt ttttcaattg gtattcaatt tctgcaatga tcgcttgttc agtcacgcct
    33301 tctttcactg ctttaaagcc aatctcaaat gcaacatcgg cccatttacc agcttcgatc
    33361 agtttgtctt tttcctctag agttttgatc agttgtagac gttggatgat ggaagttaga
    33421 tcagtgtaag aatccgttcc cgtcaagata tcagagatag catcgaagcg ctcgacagtc
    33481 aaggcgttct tttcaatccc tacattgcca ggctgaccat agcgagcacg aagttctttt
    33541 ccgatgatct cccaagggtt ttcgctgtct aaatagccac ggacatcata cgcccaacta
    33601 ctgttttgag catcttcaac ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga
    33661 gggataaaaa gagctagtac acgctcatga ggatcacttt cgtatccgga aaagtaagcg
    33721 atgtggccag ggtcgctgat ataagccaat tcgacttggt gttgtgccat ccattgacgt
    33781 agttcattga tttttacgtt attcatttgg caacctactt tctaaattaa ttttgtattc
    33841 aataattgta ccactgattt attaaaagac caacgttcta aaaactgaaa aaagtaaaaa
    33901 ttttcatgaa aacagagata aaacggttgc aattcttgag attatttgat attctatttt
    33961 agaaatgtaa gacgtatttt cagaaaacat ataaaatatg aataggagaa cacaaatgga
    34021 aaagcaaaca atcactatct atgatgtagc gagagaggca aatgtttcga tggctactgt
    34081 ttcacgtgtc gtaaatggca atccaaacgt taaaccagca acaagaaaaa aagtcttgga
    34141 agttatcgat cgcttggatt atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa
    34201 aacaacaaca gttggtgtaa tcatccctga tgtcagcaat atgttctttg cttctttagc
    34261 acgtggtatc gatgatgtgg caacaatgta caaatacaat attatcttag caaattcaga
    34321 tggcaacgac caaaaagaag tcaatgtatt gaataactta ctggctaagc aagtggacgg
    34381 cgtgatcttc atgggtcacc atatcacaga cgaaatccgt ggagaatttt cacgttcaaa
    34441 aactcctgtt gtattagctg gctctattga tccagatgaa caagtaggta gcgtaaacat
    34501 tgattatgta tccgcaacaa aagatgcagt aaacaaattg gcaaaaagcg gcaataaaaa
    34561 aatcgctttt gtcagtggtg cgttgatcga tcccatcaat ggacaaaatc gtctgaaagg
    34621 ctataaagaa gcattaagcg aaaataacct accatatagc gaaggattga tctttgaagc
    34681 acaatacaac ttcaaagatg gtctatctct agtcgatcgg atccataata gcggtgcaac
    34741 agctgtctat gtttcagatg atgaattagc catcggtatt ttggatggtt tattagaccg
    34801 tggcgtgaaa gtaccggaag aattcgaaat catcacaagt aacaattcac tactgacaga
    34861 agtagctcgt cctcgtttaa caagtatcac acaaccatta tacgatttag gggcggtatc
    34921 tatgcgccta ttgacgaaaa tgatgaataa agaagaagtc gaagaaaaaa cgattatctt
    34981 gccttatgga atcgaagaaa aaggatctac aaaataataa aaggacaacc attcagaggt
    35041 tgtgtgcctg ccgtttaact ctgcgttaca aggagcaatt tcaaaaaaca gcatctcatg
    35101 tttgatgaat attgtgactc gtgattcgga gttggcagga gaacaccgtt tatttgggaa
    35161 aaggggttat gacaaaattt tgttataatc ctttttttgt gtcttaaaaa agagagaaag
    35221 tggttgaaat tacatttatc gtatataaaa caatccgtgt atagatgctg cattttgttc
    35281 accaccatca aaaaaataag ggactgtgac aatactattg tcacaatccc ttattactgt
    35341 gtattctatt aaatcaatca tctttctcat ctgattcttt agttgcttga ttcgctcttg
    35401 cataagctga agcggtgttc cagtaatctg tatagttttc atcggtaccg ccaacgccaa
    35461 atttaaaggc gcttttcttc ggtccatttt tcgcccatag cgattcaact gtttttcctg
    35521 gtgtttggat ggatttttta ttgacagtaa cttttccagg tgtttgacca gtaaatccag
    35581 atactttttt cttcatcaca tcagatgata attcgaattt ctcatcagtg ccaaaaatgt
    35641 caggttttgc ttggtaagct tgattgatca gattggcaag ataattagcc gaacgtatcc
    35701 ttgctttgct gtccattcca gtattgtcat cgtgccctgc ccagctactg atggtaatag
    35761 acggagtaga gacaattagc catgaatctc ggtattcatt ggtagaacca gtttttccga
    35821 cccaatctgc ttttcctaga tttccgttta agcttgagat ggcatctttg aatggtgtag
    35881 tgatttttgc attgatcaca ctacgcatca tgtcattcat gatcgatgcc gttgcttccg
    35941 aatagatccg gacagagttg ctttcgtgtt tataaattac atttcctgcg ttatctgtga
    36001 tggaatcgat gatatagcct tcttcataga cgccgccgtt tgctaaagcc tgaaatccgt
    36061 tggtctgttg aagcgtcgtg acatctactg tacctaaagg agcagattca acgccccaat
    36121 tgtcatttgc cgggtagttc atctttgcca agtagtgctc gtaagcatat tgtttcgatc
    36181 caccggaatt caacacatct tgatataagt gataagccgg gatattgttg gaccattcta
    36241 gtgactcacg gacggtttgg aaggtgttcg atccttcgtt agtagcattg acgattttct
    36301 cgccagcatt ttctccttct tgccaagttg ttgcataatc tgatacacgg gattctgatc
    36361 cgataagccc catgtcgatt gcaggtccat aaacaagaac tggtttgatt gatgagccag
    36421 cttgacgttc tgtatcgaag gcatggttat tttgattctg actgtaattc cgtccgccaa
    36481 cgaatccgta gatgcggcca gttctgttat ccatcaatac atttccggtt tctacttgac
    36541 tgccagcacc atcatctaac atgtatccat aattttcaac gccattttgc aatgcagcat
    36601 agacatcttt atcgattgtc gaatggattg tatatccttt attttggatc gtttgctgtg
    36661 ccttttgata ataagcgtca ctgaccgatg aatcagacat atcagcgttg tctttttcag
    36721 ccaattgttg tgcaatgatt cttgttgctt cattcatcac agtataatac aagaattctc
    36781 tgtcagtctg ttctgcgatt tgttgtggta gaaagtcttt tgtaaggtca taggcttttg
    36841 cttcttcgta ttgttctttc gtaatttggc cttcacgata catactgaat aaaacaaaat
    36901 cttttctggc aaggccggca gaaagatctt cttttagcgc gcctgtgttc gtgtatggac
    36961 tatacgtgat cgggctttgg ggtaatccag cgatataagc agcttgcggc agcgtcacat
    37021 cttttgcatc tacaccaaag attccttttg cagcttcctg gactccagca atgttttgtc
    37081 ccttattatt acgaccaaat ggtgagacat tcaaatacgt agcaatgatc tcatcttttg
    37141 aaaagtattt ttctacttgt gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg
    37201 tttcatctgt aaggatctgc tgtttgacta attgctgtgt caaagttgat ccaccagaag
    37261 agccaatacc agtagcttca gatactaatg ctcgtaaaac agctttagga acgtaacctt
    37321 ggtgtttatc gaagtattca tcctctgtgc tgataattgc agttttcaat aaaggtgaaa
    37381 tcttatcaga tgaaatagtc gtgcgcatca gatctgtctg gatttttgat atctctgtat
    37441 tatctgcata aacaatcttt gacgtttctg taatgtttcc tagttctgtc tgcagtgctt
    37501 ttttagtcgg caattgtgtg tcttcgacga gataggcaaa atagccagcg cctattccaa
    37561 tacctagagc tgcgaacatc aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa
    37621 ctctaaaaat aatcttagga acaaaccatt tttctttttt tcttctattc tgtttttttc
    37681 gagtattttt gcgtggcaaa agggtcacct caactaatgg ttttatttcg ctaattatag
    37741 caaataatag aacaaaaaaa cagacaaggc tgtttttggc aggaatttct tttttaaaag
    37801 agttaaaacg tgagaaaatg atctttatta taaaagagtt cttaagcttt tctttagaga
    37861 aaatttcagt tactatggag aacagggcaa agaaaaatca atcattttct ttgtatatca
    37921 tcttaccaat atataggaat tgttagtaac aattgccttt caaggaggaa tccatccatg
    37981 ttattaggct cacacgtcag catgagcgga aagaaaatgc tgttaggagc agcagaagaa
    38041 gcagccagtt atggatcttc gacttttatg atctatacag gcgcccctca aaacactaga
    38101 agaaaagcca tcgaagagat gaatattgat gccggaaaag cgtacatggc agaaaaaggt
    38161 ctaacaaata tcgtcgttca tgccccttat atcattaatt tggggaatac gatcaaacca
    38221 gaaatgtttc cttttgccgt ccaatttctg agagaagaga ttgttcgtgc ggaagcttta
    38281 ggcgctactc aaattacact tcacccaggt gctcatgtag gggcaggaac agaagccgga
    38341 attgctagaa ttattgaagg cttgaatgaa gttttgacaa aagaacagac ggcacaaatc
    38401 gctttggaga caatggcagg taaaggtacg gaaatcggtc gaacatttga ggaattagcg
    38461 caaatcatcg atggagtaaa attgaatgaa aaattatccg ttacttttga cacctgtcat
    38521 acgaatgatg ccggttataa tattaaagaa gacttttcag gtgtaatgga agagtttgat
    38581 aagatcatcg ggttagatcg gcttaaagta ctccatatca atgattctaa aaatccgcaa
    38641 ggttcgcata aagaccggca cgccaatatt ggttttggaa caatcggatt cgacacgctg
    38701 aataagatcg tccatgatcc aatgtttgac aatatttcga aaatcttgga aactccctat
    38761 gtaggcaaag ataaaaagaa agcttacgca ccatatggaa aagaaatcgc catgttcaaa
    38821 gcgcaaacat tcgatccaga agtgtttcct gagttattag cagaagaagt gatactctaa
    38881 gattttctat ctgacagcag caaaaaagga ccaggcacat tgccttgtcc ttttttgttt
    38941 aagaaacaaa ggagtacttt ttgcagtctt tttcatcatg catcttctcc cccctctttt
    39001 taaatttaga caaacaaggt atactggtag aaaagtaaaa aaacgaaaag tttcacaagc
    39061 aagtggcaaa acgtgattgg aaggacgaaa agatgctgaa cactttaaaa cccttgatca
    39121 accaactaaa aaagaaacat acatacttta ttcttggcat agccctatta tttacaatta
    39181 ttcgtttgta tttatatagt aaagcaatct atggaacaga catgtcggca acctatgatg
    39241 accagctgct gattcgctat gctcaaaata ttttggacgg taaatggcta ggggagtata
    39301 cgactacaac actttccaaa ggaatctcct acagcttatt tttagtactt gcacataaac
    39361 tgcatctttc ttatgcagtt ttgttcggca tgttcagtat ttttgccagt atattggttg
    39421 cactcgcgtt taaaccgatt gtgaagaaca agtatcttct tttaagtatc tatttttatt
    39481 ttttgttttc tccgattagt tttacacatg aatattccac tcgaacatat cgtaatgccc
    39541 tcgtgattcc ggctgttgag atcattatcg cttgtttatt agctatttat taccgcaaat
    39601 acggttctag cagaaaattg ctgccatggt ttttagggct ttctgtcgct tttcctttct
    39661 tttggttcat tcgcgaagat tctatctggc ttcttccttt tactgtagta gctttgggta
    39721 tcagtttcgt ccagatcttg ctagggcaag acttttcttt caaagaagta aagaagttaa
    39781 aaaaacgact ctttcttctc tcgaaacggg aatggctgaa actttcattg tttttacttc
    39841 ctatagttgg cttttccatc gtctatcaat ggatcgaata taaaaatgag acaacatacg
    39901 gtattgcaac catcaatgac cgttccagtg gagaatttgg acgtttgatg aaacaactga
    39961 tccgaataga tgatgaaacg aacttgaata aagaaaacag tgagatatgg gtctcacatg
    40021 ctgcactaaa taaagcatta gccgcatctc ctacattcca tactgtttct acccgtattg
    40081 acgaattata tacgactcac gcgtggactc aaggtggaaa agtaaaagaa ttacccggag
    40141 atatcatctt ttgggcattg cgagatgtgg taagcgaatc tggttattac caaaacaatg
    40201 caaaagaaac caatgatttt tggaaaaaag tcaaccaaga attagaagaa gcctatcaca
    40261 agggaacaat caaaaagaaa aaagagttct atctgatggg atcaggagat ggaaagacga
    40321 tgagtgatct gccaatcgta ttcgatttcc ttcaatcagg atacgcttat gcatggcgat
    40381 ataaagattt ccatcaagga ggaaattata gtttcggaac gcaagaacaa gtcaaacagg
    40441 ctcaagaatt attaaacctt ccgctaatgg agaattggat cgatagtaat gaaccgagaa
    40501 cacctaattt ccaattaaca aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc
    40561 aagttctggc tattcccgtg ctgattgctt caatagcagg gatattactt atgatactag
    40621 gaaccgtatt tgcaaaagct gatcgtgcct attatagttc ctctttgatt gtggtcaccg
    40681 gattaatact gacacaattg gtttttcttt tcggtgtctc atggttttgt tcttatgcgc
    40741 cagaaaaaaa ggactatttt ttaagtgtct acactggtgc aggcgtgcca ttaacgcaat
    40801 tcatcgtagt aacaagcatg ctagttttca gtaaattaaa tctgaaaaaa gctttatctt
    40861 ctaaataaaa agacccttgg tagctagatt tttaacctaa ctatcaaggg tctttgtgtg
    40921 gaaaaatcag tttttattcg atttcttcta ccgtttcctg caaaagctgt tcatattttt
    40981 tgtttccttt atataaatca ccaatcgtcc atgccaacag gagaaggaca agaacaatga
    41041 ttacataagc gatgtgatca gccaatgcta gttgagcgga agtggcttgc tcgccgaaga
    41101 aactttcgat ctgtcctggt aatcctcgta agttcaagta agttagacta atcacagaga
    41161 accagccaag aagttttacg atgaatgaat ttttaaatcg gctacccatc tccgcttggc
    41221 tgttggtcat catcagtaaa ggaatcatcg aaaacggcaa cgcaaaagcc aagaacacct
    41281 gggattcatt cattaagtta ttcaaagcgg catgttcttc gatcgttcct tttgtacttg
    41341 taaacaaaac acaaatcaat acaggaatca cagaaagcaa tcgtgtgacc agtcttctta
    41401 gccaaatcgg gatacgcata tggatgaatc cttccatgat gacttgaccg gtcaacgtac
    41461 cagtaatagt agagttctgg ccagaagcaa gaagtgctac agcaaataaa gtggacaaga
    41521 taccagattt tgctacagca atcaaaatac cattgctcat agctgatgga tcagataaag
    41581 cttcatacag accaaaaaag gaaggatctt ggacagaacc ggttttgaag acagcgacac
    41641 ccatgatcaa tagcagtgca ttgactagaa aagccattga caactggata ttagaatccc
    41701 aagtagaaaa acgtacagct tgtgctacat cttcttcgtt ttggtgatcg atcttccgag
    41761 tttgtgaaac tgctgaatga agatataagt tatgcggcat gactgttgca ccaataatcc
    41821 ctaaagctcc agtcagcgga gtttcgccgc cgatgaccgg tgagtctgag aatgtatgac
    41881 tgttagggat aaagccagca aataaactgt gccaatctgg gttagacaag ccaacttgat
    41941 agacaaaaac gaataaaatg acgaaaatta agcagacaac cagtgcttcg atcttacgaa
    42001 acccgacttt tgtcagtagt aagagaacga aaacatctaa tacagtaata aagacggcaa
    42061 tcaccaatgg aatatcaaaa agtaagtaga gggcgattgc tgcaccaatg acttctgcaa
    42121 tatctgttgc cataatggct aattcagtca agatccataa aatgacacct aatgtcttac
    42181 ttgtgcgtgc tcggattgcc tgtgccaaat ccatttggct cacgattcct aattttgctg
    42241 ccatatactg taaaagcata gcaatcagac tagacatcaa aataactgac atcaataaat
    42301 attggaaatt ttgtcctccg gtgatcgaag tagaccagtt acccgggtcc atatacccaa
    42361 ctgctactaa cgcaccaggt ccagagtaag caagaagtgt tttaaaaaaa cttttcccct
    42421 ttggtacatc aactgttcca tttacttcag ctaaagataa accatttgta tgttcaataa
    42481 gtcgatgctt atgaaattgt ttattggatt gattattcaa gaaaaaacca cctttcctat
    42541 ttatctacga ttacattcta cacctcaaaa agaaaaaatt aaagaaaaag attcggtacg
    42601 cctaattttt tttcatggcg cgtcttgcta gatgacgaaa gaaattttgt tctcctctaa
    42661 aaaaaccact attttttgca tgaaactaaa gaatacgcct ttcttttttc aataaacttt
    42721 agtaaaataa gtcattgaga gcaattcgga aatagaaagg agagatttat atgttatcaa
    42781 caggtattgt cgtacttatt gctattattg ctttgctaat cggcgcagta ggtggctttt
    42841 tcctagcgcg taaatatatg aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca
    42901 tgttgcgatc aatgatgatg tcaatggggc aaaaaccatc tgagaagaaa attcgccaga
    42961 tgatgcagca aatgaagaac caagggaaaa aataggttct gacccaacga ctttttacgg
    43021 gctgattttt tcagtccgtt ttttatttcg taatttgtta gactattcag attattcagc
    43081 aaaaaagaca agcacagaca atcctaggct cttgataaag acaaaggagg agtaaaatgt
    43141 cgatttttag aaaattaggc tggttttttc gtcaggaaaa gaaacattat ttaattgggg
    43201 ttttctcgct attcgcagtg gccttggtac agttagttcc tccaaaagtg attgggatca
    43261 tcattgatga gatcgccgat aaaaatttat caataaagat cattttcggg tggatcttcc
    43321 tattgattat cgctgctttt ttacaatatc tttttcgtta tatctggcgg acgaatattt
    43381 ggggaagtgc agctcgattg gagaaagaac ttcggcagca gttattcaaa cattttacag
    43441 aaatggacaa tctcttttac caaaaatatc ggactgggga tttgatggct cacgcaacga
    43501 atgatttgaa tgcgatccag aatgtagcag gagctggtat cttgaccttt gcagattcag
    43561 tgattacggg cggaacaacg atcattgcga tgattttgtt tattgattgg cgcttaacac
    43621 tgattgcgct gattcctctg cctttgctgg ccgtcacttc acgagtgctg gggtcaaaac
    43681 tccatgatgc ttttcgtgat tcgcaagaag ctttttctag tatcaatgat aagacacaag
    43741 aaagtatcac tgggatcaaa gtcattaaaa catttggaca agaaaaagaa gatctagcgg
    43801 attttgaaga aaagattgaa gatgcaatta ttaaaaacaa acgagtcaat tttttagact
    43861 ccttatttga tccattcatt accttgatta tcggtctgtc ttatgttgca acaattattt
    43921 taggtggaaa gtatgtcttg gaaggcacga tctcgatcgg gcagatggtc tcatttatta
    43981 gttatatcgg tatgcttgtt tggccgatgt ttgcgattgg tcgattgttc aacgtattgg
    44041 aaagaggaaa tgcttcttat gatcgcgtca atgaactttt gcatgaaaag acgcatatcg
    44101 tggaacaact gggtgcagtg cagaccccgg caaaaggcga gctgaatgta gctatttcct
    44161 cttttaatta tccgaaagaa gaatcaccag cattagaaca aatcaagttt gcgatccaag
    44221 aaggagaaac attaggtatt gttgggaaaa ctggtgcggg aaaaacgacg atcatcaaat
    44281 tactgatgcg tgaatacgat gattatcaag ggaaaatcca atttggcgga cgttcgatca
    44341 aagaatattc attgaatgct ttattaggat caatcggcta tgtgcctcag gatcacttct
    44401 tgttttcgat gaccattcga gataatatcc gtttttctaa tccaaaactt tcagaagaag
    44461 aagtagagca tgcggcttct ttagcattta tcaacaatga aatcaaggaa ttaccaaaag
    44521 gttatgatac cttagttgga gaacgtggag tctctttatc tggcggacag aaacaaagga
    44581 tttcgattgc tcgagcattg atcaaaaatc ctgaattatt aatattagat gatgcactct
    44641 ctgcagtaga tgccaaaaca gaagaagcta tcttgcataa tttaaaagaa ataagaaaag
    44701 agaaaacaac tatcattgca gctcaccgtt taagtagcgt gatgcatgca aaagaaatca
    44761 ttgttgtcga tcaagggaaa atcattgaac gaggaaccca tcatgagctg cttgcactag
    44821 gcggctggta tgaacaaatg tgggagaaac agcaattaga agcgaagata gaaggaggcg
    44881 aggcctgatg gatgaaaatt atcaatcgga atggacaaaa tcgattccac ttaaagaaca
    44941 gaaaaagatc atcaaacgac tgatgaagtt cgccaaacct tttagaagaa cgttcatcgt
    45001 tgctatctta tttgcgtttg ctttgtctgt gatcaatgtc ctattgccaa ggatcatcca
    45061 aacttttatg gatgatcatc tggctaaaca gtcggctacg acacaagtta ttttgttttt
    45121 tgctggtatg tacttatttg gagtcatagt aaaaagtatc atttggtttt tccaatggta
    45181 tttgtattcc atggcctcat tgaagaccta tcagtatatt agggtaaaat tatttgaaaa
    45241 gctgcataca ctaggtatgc gttattttga tcagacaccg gcaggatcaa ccgtttctcg
    45301 agtgacgaat gacacagaaa cgctatttga attttggtac gtctttttga tggtattgac
    45361 tgggatcttt gcagtcgttt cttcattcct tgccatgttt caaatcaatg gagaaattgc
    45421 cttgtggact ttggtgtttt tgccgatttt aggaatcgtc atctggtatt accagaaatt
    45481 cagttctcgt atttaccgaa atatgcggga aaaattaagt cagctgaaca cgaaattgaa
    45541 tgaatcgatt tccggcatgc gtattatcca gcagtttcgc caagaagctc gtttagctaa
    45601 agaatttgaa caagtgaaca atgagtattt agacacgcgt tatgcgatga tcaaaacgaa
    45661 ttcgattctg ttaagtccga tcatcaattt tctatatgca ttggcgattg cgttggtgtt
    45721 gactttgttt gggatcgatg cgttgcattc tcccgtagaa gttggactga tctatgcatt
    45781 tacgacctat gtccagggat tttttaatcc aatgtctcaa atgatggact ttttgagcat
    45841 cttcacagat gggatggttg ctggtagtcg tatcttgaag attatggata cggaagaata
    45901 tgcaccacaa caaaaccctg gagcagatca aatgattact tcaggtaaga ttgagtttcg
    45961 caatgtaagc ttttcttatg atggagagca caatgtatta aacaatatct cttttgttgc
    46021 taatccggga gagaccgttg cattagtggg acacaccgga agcggaaaaa gctcgatcat
    46081 caacgtattg atgcggtttt acgaatttta tgaaggtcag atattgattg atgatcatga
    46141 cattcgagaa tatccaatgg aagagttacg taagaaaatg gggctggtct tgcaagatgc
    46201 tttcttgttt tatggggata tcgcaggaaa tattcggatg atgaatccag ctatcacaga
    46261 cgaacagata cgccatgcgg ctgagtttgt tcaagcaaac aaattcatcg atgaattacc
    46321 aaaaggatat cactcaaaag tgatcgaaca tggtgccagc tattctagtg gacaacgaca
    46381 gctgatcact tttgcgcgaa caatcgtgac tgatcctaaa attctagtat tggatgaagc
    46441 aacagcaaat atcgatactg aaacagaagg attgatccaa gaaggattgg caaaaatgcg
    46501 tcaaggacgg acaacgattg cgattgccca tcggctgtca acgattcgag atgcagaatt
    46561 gattttagtt ttagacaaag gtcatatagt agaacgcggg aatcatgaaa cacttctagc
    46621 gcaaaatggt ttgtatgcag atatgtacaa actgcaaagc agtggtatat aaaagatgtt
    46681 gtgacagtag aattttgacc taagcactag agtgaaaata aaacatagct tctcatattt
    46741 ttcatctgtg agagttagtg tcgtagtcac cagatttaaa aaaagattcg acagctagtc
    46801 agaagtgcct aatagccact aagaaagcgt tgacaaaagc atttgctttc gataatataa
    46861 agaaaacgat acaacttgga tagtgattgc ctgatgcaag ctaaacctga tgagtctccc
    46921 atggtttcat ggacggcttt ttgggttttt ctttttgttt tggatacagc cctatacatc
    46981 atggaaagga gcaaaacatg ctgattgaga aagtactgaa taacaacgtc gtaataacca
    47041 gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg tttggcattc aaaaagaaag
    47101 tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt tcagttgaca aatgacgggc
    47161 tttcacaaca gttccaagaa ttggcacaag cgattcctct tgagtactta gaaatttctt
    47221 gtgaaatcat tgaatataca aaagaggctc tacaagtcca gttgaatgat tctgtgtata
    47281 ttgcattgac ggatcatttg tatacggcta tcgaacgtca caaaatggga atatttgtac
    47341 caaatgtatt gctgtatgat atcaaacaac tatttcctaa agagtatgcc atcgggaaaa
    47401 aaacagtgaa gaaaatctcg gataaatacc acatcaagtt atcagaagat gaagccggat
    47461 ttatcgcatt gcatttagtc aatgcacaag gcattcaaag ccagcaagac aatacctacc
    47521 tgtttactat aacaatacag gatattctaa atatcgtccg ctattactat aagattacat
    47581 ttgatgaaga ttcagtccat ttttttcgat tcgtcacaca cttacgtttt tttctgacac
    47641 gcgtcctaga tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg tttcaggtta
    47701 ttcaaaaaaa atataaggat acgtttggtt gcgttgagaa aatagcagac tttttagccg
    47761 agacgttcaa ctaccagatg tccacggacg aaaaaatgta tttaacgatc catactgcgc
    47821 gtttagtgca tgagaatcgt tgtaaataaa aaaaggagga taaattaaca agaacgtatg
    47881 aaataagggt agtgacattc agttggctaa accttccact aataaattgg agggaataga
    47941 aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg ggaaggaaaa
    48001 tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa acgaagaaaa
    48061 agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc gagcgggagg
    48121 gcagtatcaa gttgtcattg ggaatcatgt gccagtcgtg tttgaagagg ttataaaagc
    48181 aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt ttgatatgct
    48241 tattgatgga atatcgggct gtttccagcc attccttgct atcctagctg ctggcgggat
    48301 gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg gttctggcac
    48361 atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg ttattatcgg
    48421 tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc ttggcgcagc
    48481 attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag caccactaac
    48541 gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat tcggcattcc
    48601 ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg tgttgttagc
    48661 tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga atttcttcgt
    48721 tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg gaccagtatt
    48781 cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac tttcgccagt
    48841 tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg gtctccattg
    48901 ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa atattatgac
    48961 acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct actttaagct
    49021 tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta ttgtggggat
    49081 tacagagcca gctatttatg ggtttacatt accacgtaaa aaaatattta tttttacctg
    49141 tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt cttggaatca
    49201 aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg atttaacaga
    49261 tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatccttta cgctgacttt
    49321 tttcttttgg aaggatgaag caggtgaaac agatgatata cagaaaaaat caggtaaaga
    49381 aattgtaaaa acaccgatac aaggacaaat cgctccacta aatgcagcaa aggatgcagc
    49441 ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag gagaagtacg
    49501 tgcccctttt gacggaacag tcatgaccct gttcccgacg aaacaggcaa ttggaatggt
    49561 ttctgaaaca gggctggaat tgctgattca tgtcggattg gatactgtgc agttggaagg
    49621 gaaatatttt gaaagcttag tgcagcaagg ataaagagtg aaacaaggcg atctgttgct
    49681 acgcttcgac aaaaagaaaa tcgaaaagga aggatacagt ttagaaacac ctgtgatcgt
    49741 caccaattac acggattatt tggatatttt ggaaaaccaa ggggagacgg tcgggccaga
    49801 cgatgcactt atcaccgcac tgacctgaac ggttaggtga agaatggact aggcttaact
    49861 attcttttta ttccacttct ttattttcaa aattgaaagg ctctttcatg atggctgagc
    49921 tgatagggcg tttatcgaat ttttgaccga cagaaattag atggactaaa ttctgttgat
    49981 aattttctgc aaaaacacaa ctatataaca cgtctagaag ataacaaatt gatgtattga
    50041 tcgtaaaatt accaattttt gaatacagtc gctcgcgtgt cgtaagtcgc aaagcggctg
    50101 tactcattgc agatagcgtt gaatcaccga tactggtcaa agctaaaatt ggaacatgat
    50161 tttgtttcaa tatttctgcg gtacgaagga tcacattgtt ttcacccgta taagaaatca
    50221 gaatcccgca catatcactc gtgcaattgt acgcttcata gaatgattca ccaaaagtgg
    50281 tgacaatcgt gacatccatt ttgatgcgct tcattttcag catgaaatct tgagaaatca
    50341 aagtattcga gttactggcg aaaatcacga ttttttttgc ttgaagcatt agttgtttgg
    50401 ctttttgcag ttggtcatga tgaatgagag aaagtgtatc atgaatcgtc atctgttcta
    50461 aagaagccat tttatttgcg atcgtcataa taccgtctgt ggtggaaaaa ggaagattcg
    50521 catctacgtc ttgaaaattc ccttcaagat aggctgtttc agctaaaaaa gcattacgaa
    50581 aactgaccca gccatcaaaa cccatttttt ttgctacacg gatgagtgta gaggggtgga
    50641 cataacatcg ctcagcaatc gcttggatgg tcatttcgcc aagtgctgga gaatcgctta
    50701 agagaaactg gatcacttca ttttctgaag gagaaaagga gatttctttg attttttcac
    50761 gtaataacat gtagatacct tcttcttatt tttttacgta aattataact tttttgaaaa
    50821 tgatttgtaa acggatttat actatataac ttaaaataga cattttttga caagatattt
    50881 acaaaaatcc actagaaaac agtaaatttt gtaaatgtta ctggaaagaa acagatttct
    50941 attgtttgtt ttgtttacaa aaaagataat gaaagtgtaa aaaagtaaag gagtgagaac
    51001 atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac ccccgagttg
    51061 atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg gctcgtagat
    51121 gtagaagaag gaaaagaaaa gcttgagatc gttagtcaga tgtctcagcg tatgtgggat
    51181 gcttctccgt atgaagtcaa gatctatgca actctggatc gggaagaagc attgaaagat
    51241 gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat caaagacgag
    51301 cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg cgggatattc
    51361 aaagcttttc gtaccgttcc agtcgttttg gcaatcgttg aggacatgaa gcgtttatgt
    51421 ccaaatgctt ggctgatcaa tttcgcgaac ccaagtggga tggtgacaga agcagttatc
    51481 agatatggca aatgggataa agtgattgga ttatgcaatg tgccagtgat ggcgatgatg
    51541 acagaaccag caatgcttgg caagaaaaaa gaagagctga tctacaagtt tgccggtcta
    51601 aatcatttcc attggcacaa agtagcagat aataaaggaa atgaccgcac ggcagaattg
    51661 attgatcgtt tatacgatga agagaatgga ttaccgaaaa atatctttga tatcccgttt
    51721 ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg ctattactat
    51781 cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg tacaagagcg
    51841 cagcaagtga aagaaacaga agcagagcta tttgaattat acaaagatcc agaattagac
    51901 cataaacctg aacaattgca aaaacgaggt ggcgcttatt attcagacgc tgcctgtgaa
    51961 acgattgctt caatctatgg aaataaagaa acacagatcg tggtatctac aaagaatgat
    52021 ggagctgttc ctgacctgcc ggcagattgt gtagtagaag tcactgccta tgttggcgca
    52081 caaggagcga gaaatgtagc gtttggttct cttcctgctg ctgaaagagg ctggttgcaa
    52141 gtaatgaaga atatggaatt gctgacgatc gaagcagctg tgactggtga ttatgggaca
    52201 gctttacagg cgtttacgat caatccactt atacgatcag gaaagacagc aaaacggatc
    52261 atggatgagt tgtttattgc acaccagcag tatttaccgc aattcaagga gacaatcagc
    52321 cgattaaaag aagaaggaat cgaagttcaa gatgaactgg caagagaatt atcaggtaat
    52381 acacatataa tggaggaagt ataatgggat tcagaaaaga ttttttatgg ggcggtgcta
    52441 cagcagccaa tcaatgtgag ggcggttatg atgaaggtgg acgcggacta gcaaacgtag
    52501 atgttgtgcc aatcggaaaa gaccgttttc ctgttatcac cggaaagaaa aaaatgtttg
    52561 aatttgatga ggatcatttt tatccagcaa aaattgcaat cgatatgtat catcgttata
    52621 aagaagatat tgctttgttt gcagaaatgg ggttcaagac ttatcgaatg tccatcgcct
    52681 ggagtcgtat ttttccaatg ggggatgaga aagagccgaa tgaagctggc ttgaaatttt
    52741 atgaggatgc ctttaaagaa tgccaaaagc acggaattga gccacttgtg acgattacgc
    52801 attttgattg tccaatgcat ttgatcaaag aatatggtgc gtggcgttcc agagaactcg
    52861 ttggttttta tgaaaatctg tgtcgagtca tcttcaatcg ctataaagga cttgtcaaat
    52921 attggctgac attcaatgag atcaatatga tccttcatgc accattcatg ggcgcagggc
    52981 tgtattttga aaaaggagaa aatgaagaac aaatcaaata tcaagcggca catcatgagt
    53041 tggttgcaag tgccatcgca acgaagattg ctcatgaagt agatccggag aacaaagtag
    53101 gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa gatgtttggg
    53161 cttctagaaa atcagatcga gaaaattatt tcttcattga tgtccagtca cgaggagaat
    53221 atcccgctta tgcattgaaa gaaatggagc gaaatggcat tcagatcgaa atggaaccag
    53281 gggatgccgc attgctgaaa gaacatactg ttgattttat ctctttttct tattatgctt
    53341 cccgagtttc gactaccgac aacgaactct tggaacagac tgcaggaaat atctttgctt
    53401 ctgtcaaaaa tccatatctg caagcaagtg aatgggggtg gcaaattgat ccattaggct
    53461 tgagaataac gatgaatgat atttatgatc gttatcaaaa accattgttt attgtagaaa
    53521 acggcttagg tgcagtcgat acgccagatg aaaatggtta tgtggaagat gattaccgaa
    53581 tcgattacct ggcagcgcat atccaggcga tgaaggatgc agtagaacta gacggagtcg
    53641 atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt accggtgaga
    53701 tgaagaaaag gtacggattc atttatgtcg atcgtgataa ccaaggaaat ggcacattag
    53761 cccgttcaaa gaaaaaatca ttcaactggt ataaaaaagt aattgattca aatggagaaa
    53821 atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc ggatttttcg
    53881 agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt cgcattggct
    53941 gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat gtaatcgtta
    54001 ttggcgcagg tcctgctggg atgactgctg ctttatatgc ttcacgttca aacttatccg
    54061 tattgatgat cgaacaaggt gcccctggcg gccaaatgaa caatacggca gaaatcgaaa
    54121 attatcctgg attcgattcg atcatgggcc ccgatctagc ttataagatg tatgaaagtg
    54181 tctcaagatt tgggacagaa aatgcttatg gaatcgttca agatatcaag gatcacggca
    54241 gccataaaga agtcatctgt gaagataaaa gttacgaagg aaaaacagtc attatcgcaa
    54301 caggatgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct ggacgaggcg
    54361 tttcttactg tgcagtctgt gatggtgcgt ttttccgtaa caaacgtcta gtagtcatcg
    54421 ggggtgggga ctcagcagta gaagaagcca tctatttgac acaatttgct tctgaagtag
    54481 tgatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac cgtgcatttg
    54541 caaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt ggaaacgaaa
    54601 tggtagtgac aggtgtgaaa gcacgtaatg tcaaaaccgg caatgaattt gatttagcag
    54661 cagacggagt attcatctat gttggactgg atccattaac tgcacctttt agagaagcag
    54721 gtttgacgaa cgaagctggc tggattccaa cagacaacga aatgaaaacg aatatgccag
    54781 gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact acagctgttg
    54841 gtgacggcgg tattgctgga caacaagtgt ataaatactt ggagtcttta gaagaaacaa
    54901 cagcataagt ttaaaaagag tagaagaacg ggttcaattg ttcttttact cttttttttc
    54961 gcgtgaaatt tactgaatct gatttatgcg aacacgtcca acaccttttg ataaaagaat
    55021 cctttcttga tttaagcgtt ttttggtcct catactttca tatctaataa gtgaatgcta
    55081 tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg gaacaagtgt
    55141 atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa ttaaatgatc
    55201 tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa ttcggtactg
    55261 caggtatgcg gggaatctta ggacctggga tcaatcgaat gaacatctat acggtaagac
    55321 aagcaacaga aggactagca cgtttcatgg atacacaagg tgaagaaaca aaacgccgag
    55381 gtgtcgctat cgcttatgac tcacgccatc agtcaccaga atttgcaatg gaagcagcca
    55441 aaacactagc acatcacaat atcccgtctt atgtattcga aagtctgcgt ccaacaccag
    55501 agctttcttt tgcagttcgt catttgaaga cgtttgcggg aatcatggta accgcatctc
    55561 acaacccagc ggcttacaat ggatataaag tctacggaga agatggcgga caaatgcctc
    55621 ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg ctaaaagtcg
    55681 aagtattatc tgacgaagaa gctaaacata gcgggttgat cacgatcatc ggagaagaag
    55741 tggacgctgc ttatctagaa gaaatcaaag cagtgacgat tgatcgtgaa ttggttgaaa
    55801 caatggggaa agatttgaaa cttgtgtaca cgccacttca tggaacaggg aaaatgttgg
    55861 gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttccc gaacaagctg
    55921 tggcagatcc agatttcact acggtcaaat cgccaaatcc agaagaacat tcagcttttg
    55981 aatatgctat acgacttgga gaaaaagaag gagctgactt gctgattgca acagatcctg
    56041 atgcagaccg tttaggcgca gcggttcgtt tgccagatgg ttcttatgaa gtattgactg
    56101 ggaaccaaat cggagcattg tttgctcaat atattttgga agcacatcaa tcaagaggca
    56161 ctttaccaga aaacgcagcg gtattgaaat caatcgtttc cagcgaattg cctacagcaa
    56221 ttgcggaaca ctacggtgct accatgttca atgtgttgac agggtttaaa ttcattgctg
    56281 aaaaaatcca acaatttgaa gaagaccatt cacatacttt tatgtttgga ttcgaagaaa
    56341 gttatggtta tttagttaaa ccttttgttc gcgataaaga cgcgatccaa gcattgcttt
    56401 tattagcaga agtagcagct tactataaaa aacaaggcaa aacgttgtat gacggcttgc
    56461 aaacgatttt tgaaacctat ggctatttcg cagaaaaaac gatttctgtg acaatgagcg
    56521 gacaagaagg ttctgcgaaa atctctgctt tgatggaaaa attccgtaaa gaagcaccga
    56581 cagaatttgc cggagcaaaa gttattcaaa cagaagactt taaagaattg acgagaacag
    56641 accgtgaagg aaacgtagaa aaactaacaa caccgccatc cgacgtattg aagtatacat
    56701 tggaagaaaa tggctggatc gctatccgcc cttcaggaac tgagccgaaa atcaagtttt
    56761 atattggtgt aaaaggaagc tctacagaag acgcagccga aaaagtagcc tcttatgaag
    56821 cgattatcaa aaagatgaca gaagaataaa aaacttgaga ctgatggaaa aagtggtaaa
    56881 ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca cgcaaaaatc
    56941 aaaagtagag aaagtcagtc aattattcaa aatgttgagt gatcccactc gtcttaaaat
    57001 tctgctatat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag cagtggaaat
    57061 ggagcagtct gctgtatcgc atcaactacg tttattaaga gaaaatcatg tcgtcaagtc
    57121 tcatcgcgag ggaaaagcga ttttgtatag tcttgatgat tctcatgtac tggatatttt
    57181 gaaccagaca ctcaaacatg tggaacatca ataacgatag gtgaaaatag ataaacactg
    57241 gagtgtgaca acgaagaaat ttcgttgtca cactccagtg ttttttaaga aaaagacaaa
    57301 aatgattaat cgcttttttc tgttgtttca gttccataat aactatgatc ttctaggtct
    57361 aaagcattca aaataagcga agccgtttct tcaatagata aagatgccac attgatcact
    57421 acacatccta gtttttcata aagatcattc gcaaaatcga gttcagctct aattttttcg
    57481 atatcggaat aagcagtatc agggtttaat ccgtaagagc gcatccgttc tttacggata
    57541 ttatttaaaa tatctgggtt attcgttagt cccacgattt ttttaggatc gatttcccac
    57601 agctgtttgg gaatatgtgc ttggggaatc aaaggtaaat tagcaacttt taaattttta
    57661 tttgctaaaa acaagctcaa aggcgttttg gaagttcggg atacgcctag caaaacgaca
    57721 tctgcttcta aaaaaccacg aggatctttc ccatcatcgt attttacagc aaattccatt
    57781 gctttgatcc gtttgaaata gttctcattc aataaatgga gggcaccagg ttgccgaatt
    57841 ggcttgatac cagttaaacg ttcaatttct gatatcggtg gcgtcatcac atcgaaacta
    57901 aataattgat gttcttcaca aaaagaacgt gttatctcga tcagttcttg attgattaat
    57961 gtatgcagga caagtgcttt ttcttctaat gcatcttgca atgcctgcct taatgttttt
    58021 tcatctgtca cgaacgttct gcggaatagg ttgaactcaa cggtaggata ctgagccata
    58081 gtagcttgcg ctagttttgt cgcagtttct ccagcagagt cggagatgac aaaaaaagta
    58141 acgatttctg tagaatcgga agacatggat aaaacctcac ttgcattttt tttatgttta
    58201 ttataccata ctctagaatg cgtacttcgt aatcattttg atttggagga ataagtcatg
    58261 aaaaaacaga cattgatcga acaatcatta gaaacattga aaaataacgg ttttaaatat
    58321 actaaaaaaa gagaagcatt gttaacttat ttagtgaagc gtaaccgcta cgtatctgct
    58381 aaagaagttt ttgactatat gaatcaggaa ttcaaagggg taagttatga tacgatttac
    58441 cggaatctcc atgattttgc agagatcgat ttactggaag aaaccgattt aaatggtgaa
    58501 atgaagtttc gttttcattg ctgtcagggg gaaatcggcc atcaccatca ttttatctgt
    58561 acagtgtgcg gaacaacgaa agaaatccat atgtgtccaa tgaattattt cgaagaacag
    58621 ctaggtggat gcacgatcga agggcataga ttcgagattt tcggaagatg tgaaaattgt
    58681 caataaggat ttaaataaaa taacatttga taatttaaaa aaaaagcaag atttccttga
    58741 cggtcaaaac agcattagat ataatattta caggacagtc ttgttttttg agatatattt
    58801 caataacaat tacggttact ttttaagctc ggagggaggg aattaacatg tcaaaaacag
    58861 tcgttcgcaa aaacgaatca cttgacgatg ctcttcgtcg cttcaaacgt tccgtgtcaa
    58921 aagcgggtac tttacaagaa tctcgtaaac gtgaattcta cgaaaaacca agtgtaagac
    58981 gcaagaaaaa atctgaagca gctagaaaac gcaaaaaatt ctagttctgt gatgaattgg
    59041 agttggtagt gtgtcactac taagtagatt aaacgatgac atgaaagcag cgatgaaagc
    59101 gaaagataag gaatctttac aagtcatccg aatgatcaag tcatctatac aaaacgagca
    59161 gattaaagaa ggtcacgact tgacagaaga agaagaacta acggtgttgt ctcgcgaaat
    59221 gaaacagcga cgtgattctt tgcatgagtt tgaagaagca ggacgtgacg atttagctga
    59281 aaaagtaaaa agtgaaattg taatagtcga aaaatatatg ccggaacaac tttccgacga
    59341 agaaattcga caacttgttc aagaagcaat cactcaaact ggtgcttcat caatgaaaga
    59401 atttggcaaa gttatgggtg caattatgcc taaagtcaaa ggaaaagcag atggaaacca
    59461 agtgaatgcg attgtgaaag aactattaca atcttaattt tatcatttaa actgcggaca
    59521 gtgattgtcc gcagtttttt gtgttcgtaa aaaaagaact aaaaaacagc tagaagaaag
    59581 aataagattc tttcttctag ctgttttttt cctgtttaat atctcttatg ttaaattgtg
    59641 tttgatttgg ttattcttct ggtagttcat ttgcttcttg tttataccct gataggcgat
    59701 aggcgtaata aaagagtaag ctgccagcgt aaccaagtaa cgttgggacc atccagccaa
    59761 gtcctaatgc agcaaaagga acgtgatgat gataaaatcc tagtaagctt gctacgacag
    59821 tttgctgact cactaatgca ggcgaatttg ccaacatatc caatacggct ggaacggcag
    59881 aaaagaacat cgttacttga tagacaaatg gactcttttg gaagaatttt gctgttaagg
    59941 aaagcaagat taacgcaagt gacaatggat acaaaagcat caatactggt aaagaccatt
    60001 ggatgatatt atccaatccg gcattcgcaa cgacaaaaga gacaaaagtc gttaaccgca
    60061 gccatgtcat ataactgact ttagggaaca gtttatggaa atcttgtgca aaagaaacaa
    60121 cgagtcccat agcggtagtg aagacaccta atgtgaccat tacccctaag aagctagcac
    60181 cgaagttacc taaataaaaa ttagtgatct gagaaagtgc agttccgcca ttttctgata
    60241 aaggcatctt attcaaactc atcgcaccga gaaggaccag accaaaataa atcatgactt
    60301 ctaacgcaac gctcaggcta ccagcttttg ctgttaattc tgtgactttt ttatctttaa
    60361 aaccaagatc acgaactgca tggacaaaag tgacacttaa agcaagtaaa gctactgcat
    60421 caaccgtgtt gtatccttct aaaacgccat ttgtcaaagc gttcgtctga taagaatctg
    60481 cagcagattg gtttagattc cccatgggtt ggaataaaga aacgacaaaa atgatagcta
    60541 ataatgcaag aaacaaggag tttaaatact ttccgacata tttgaccaaa cgtgttggat
    60601 gtacagtaag gaaataagca agtaaaaaga aaagagcaga gaataaaaac atacctaatg
    60661 aatcatattt ttcaggcaaa aagggtttag cagccatttc aaaagcagtt gctgctgtcc
    60721 gcggtgtacc aaagaatggt ccgatcgtta aatgaacaag gatcaagaac aaggcagcat
    60781 accattttcc gacaggttta gctaagtcat acagaccatc actttttgtc actactacag
    60841 ctaagatagc taataatggg aaaagagcgc ctgagatggc aaatcctaca ccagcgctga
    60901 tccagtttga tccagccatt tgacctaagt ggacaggaaa gatcagatta cctgacccga
    60961 agaacatccc gaagataagt gaactgacca tcaataattg cttccatgaa taagtttttg
    61021 tttcttccat aaatttttcc tccaataatt atcattttga gtaacaaaat aaacgttgca
    61081 gaaactagaa atgccactaa tatcagattc catgaaaacc accaccttaa aatataaaaa
    61141 aaagcccttt tccacttggg aaaagggctc tagattctag aacggtacca ccttttttcg
    61201 cttaaaaaag cttcttcatt cacggacgct tttcgataaa gttatccgct agcctgataa
    61261 tggaggcagc cacaatgatc tacgttttcg acattgactc ggaaatgatg ttcgtctatt
    61321 cgatttattg tcatttctca cttacatgac tcactgggaa taaatcgctg aatagcctac
    61381 tgttttcctc gacgctttgc tgtatattta tttgttgacg ttataataca cctgtttttt
    61441 ttgaaatgca agaatatttc aaatattttg ttttcttttt ttgaaacaag aaaaataccc
    61501 agagaatact tgctaagtta gacggaatac ttgtcctcta tcttatgcat agcttacatg
    61561 atcagctttt tttattcaaa cgagaccttt agagaatgga agcagctatt ttttaacgtt
    61621 tcttggtact tatggttaag caactctttt agaacctttt cttatttgct atatagtttc
    61681 ttctgagtat ggtaaaatag gaaagaatga atcgaaaaaa aggggatgtc tatttttgac
    61741 ggaagagtct agttctttag agattagatt gacggctgct gatgatgtca gcatgctttt
    61801 aggtgcacac gacaagcata taaaactgat tgaagaaaca acagaaacaa ccatccatac
    61861 acgtggagaa atcattcaga tcattggcga acaatcccag atttccttgg ctgcctcagt
    61921 cattcggaca ttgcaggaat tgatcaaacg aggaatccat gtaagtacac cagatgtcgt
    61981 gacggcactg aaaatggggc aaaaaggcac attagattat tttgttgaaa tgtatgagga
    62041 agagatcgtc aaagaccgta atggaaaacc aatacgggta aaaaacagcg gccaaaagaa
    62101 atatgtcgaa tcggtagcca aacatgatat cgtatttggc gtaggaccag ccgggacagg
    62161 aaaaactttt cttgcagtag tattagcaat cgctgccttg aaaaaaggtg aagtgcaaaa
    62221 aatcattttg acgcggcctg ctgtggaagc aggggaaaat ctaggctttc ttcccgggga
    62281 tctaaaagaa aaagtagatc cttatctgcg tcctgtttat gatgctttat accaaatttt
    62341 tggattagat catacgaacc gattgatgga acgaggcgtg atcgaaattg ctcctttagc
    62401 ttacatgcgg gggcggacat tggatgatgc attcgttatt ttggatgaag cacagaacac
    62461 gacagttgct cagatgaaaa tgttcctgac acgtcttggt tatcaatcaa aaatgattgt
    62521 caatggagat acgagtcaga ttgatctgcc tcgagggaca acaagtggtc ttgttcatgc
    62581 agaacgaaca ttaaagcaaa tcaagaaaat cgattttgtt aattttgaag caagtgacgt
    62641 tgttcggcac cctgtagttg ccgaaatcat caaagcttat gaaaaggcag atctccatca
    62701 agaataagct ttgcttagga ggataagcac atgttgaata aaacactgca gaaactacaa
    62761 gaaaaacttg gaaaggcagc attgcctttc ctgctgttct tgttttttat cattatctgc
    62821 ggcgttacat ttagtagtgt caaacaaaag gccaatgatt tcagagaagg ccaagtagca
    62881 gaagaaagta tccgtgcgaa caaaacgatc gagaacacgg aagaaaccga acaaaaaaga
    62941 aaactggcag cagaagcagt cactccagaa tacacttacc aaaaagatct tgcagatgac
    63001 cagaacaatc ggatcaagca actatttgaa ttgatcgata aaacgaatga tgccatcaac
    63061 aaatcttatg acgaaaagat tgataaggca aaggaagatg aaaatatccc aaagcctact
    63121 acagaagagc ggatcgccgc gttgaagaaa aattttgaaa atgtgaatgc agaaaatgtt
    63181 gcgttttatc aaaaactacc gaacaatttt tattcgacga tatttgaaat gactgaaact
    63241 gagatccata ctgtgaggga tgagagtctg aagctcattg acgaacagat gagcaaacaa
    63301 gttcgtgaaa gtgaattgga agcttttaaa caagaagcag aagagcaaat ccaatatttg
    63361 aatgtcacac cagcacaaca acaaatgatt cgctacttag tggatcaagg tatcgtagtg
    63421 aatgacgtat tgaatgagaa aaagacagaa gaattgaaac aatccgcacg agaagctgta
    63481 caaccagtga tgatcttcca aggagaaatc attgttcgtg aaggtaatca aatcgatgct
    63541 tcagctatga aaaaattgga attactagga ctgactaatc aaacaacttc gatttttccg
    63601 ctaattgcca tggttttagc tgttttgctt caaatcggcg tactgattta taattcactt
    63661 caattccaaa atgcaggaga aagaacaaaa tacgtgcttt tctatgtgac agctatgtca
    63721 gtcagcatag tgctgatgaa atttttccaa ttattccaaa cggaacaagc tacttatatc
    63781 ccgctgtttt atcccgctgc ttttgcacca cttgttttaa gctttttctt gaaccgaaga
    63841 gcagggatca tggctggtat tttccaagca gtatctgcac ttttcatttt ttatgattcg
    63901 attggcacga actttctaac gattatcttg atgtcttatg ttttctctgg gctcatggca
    63961 acgattgtca gacgcaaacg gatctcagaa caaggattat ctgctgccat gtgggtcatt
    64021 atttttcctg tgatgatgga tgtcattttg atcgtctatc aaggaatgag ctttggtgat
    64081 gcaacgactt ggttaatgct tgtctgcggg ttcactggtg cactcttctc attcttgctg
    64141 acgatcggtc tacacccata tattgaattg atggtcaatg atgacagtgt aattgtatta
    64201 aatgaactca gcaatccaaa tcatccttta ttgaaacagt tgctggaaga agcgccaggt
    64261 acttatcatc atagtatgat ggtagctaat ctaagtgcta acgctgtagc agaaatcggc
    64321 ggacgttctt tgctaactcg agtagcttgc tattatcatg atatcggcaa gatcaaacat
    64381 gctagctttt tcgttgagaa cttgccttca ggagcggaaa acccgcacaa tttcttattg
    64441 ccagaagaca gtaaacatat tatctttgga catgtgacag atggagccaa aatattggaa
    64501 gattataata tgccgcaaat ggtgatcgat atttgcaaac agcatcatgg aaccacgcta
    64561 atgcgttatt tctacatcaa agcgaaagaa cgcaatccgg aagtaacaga agagcaattc
    64621 cgttatcctg gaccaaaacc gcaaagtcgt gaagcaggga tcgtcaatat tgctgacagt
    64681 tgtgaggctg cagtacgggc aatggatcat ccgacgagcg aaaaaatcga aagttttgtc
    64741 cataatctta tcgaagagcg tatttccgat ggtcaattag acgatagtgg actgaccttg
    64801 aaagaaatcc gaaaagtgga aaaatcattg atcagcggtt taagtagcac cttccattct
    64861 cggatcaaat atcctaaaat gaaaacagaa gttgagaaga taaaggaaga acaagaagaa
    64921 tagaaagaag cgatgtataa tggatattac ctttatggat gaaacaaacc aagtctcaga
    64981 agagaaaatg aaagaaatcg atgacttgct gcaatttgca gcatcttatt taaagctgcc
    65041 agaggaaaca gaaatgtctg ttactttcat ggacaatgcg gcaatccaag taatcaatcg
    65101 tgattaccgt ggaaaagatg caccgacaga cgtcatcagt tttgctctgg aagaagaagg
    65161 cgaagacgaa atcccagtga tttttgaaga tggagagaat ccattgcctc gtgtactagg
    65221 agacatcatg atttctattg aacgtgctgc tgaacaagca gaagcttacg gccattcata
    65281 tgatcgggaa cttggctttt tagcggtaca tggattcttg catatcaacg ggtatgacca
    65341 tatgacacca gaagatgaga aagaaatgtt tggtttacag aaagagatct tggatgccta
    65401 tggactcaaa agataaaaac gtagagaaaa acaaacattt cctgatgtct gtggaatttg
    65461 ctgtccaagg agtcaaaacc gtttttgagg aagaacgcaa tatgaggaaa catgttgcgt
    65521 tcggtatcct tgctttattt atgggcgtga ttttccagct tgatcggata gagtggttat
    65581 ggctgctttt agctgttttt ctcgtttgga tagtagagat catcaatact gtttttgaaa
    65641 atgtagtgga catgtttacg gattttcatt ttcatccgat tgggaaaaaa atcaaagata
    65701 tggcagctgg agcagtttta gtgaccgctt gttttgctgt catcattggg ttgattttat
    65761 ttgtccctaa aatatatcat ttgttgttta attaaaaagg agaacgtata tgccagaaca
    65821 taaatcaggg tttgtcgcaa tcgtcggccg cccgaatgta ggaaaatcca cattattgaa
    65881 ccgaatcgtg ggtcaaaaaa ttgccattat gagtgacaag gcacagacaa ctagaaataa
    65941 aatccaagga gtatatacta caccagaagc tcagctgatt tttatcgata caccaggaat
    66001 ccataaacca aagcatcgtc taggagattt tatggtagaa gcagcttaca gtgcgttgaa
    66061 agaagtggat gcagtcttat ttatgatcag tgctgatcaa aaacgcggcc ggggagacga
    66121 ttttattatt gaacgtctaa aaaacgtgca ttctccagtt tatctgatta tcaataaaat
    66181 cgataagatc catccagacg agttgctggg aatcatcgaa gattattcga cgcagatgcc
    66241 ttttgcacaa gtgattccga tctctgctac agaaggaaac aatgtcgaac gcttaatgga
    66301 tgtattagtc agtgaaatgc cagagggccc acaatatttt ccagatgatc aagtgactga
    66361 tcatcctgaa tactttatcg tttctgaatt gatccgtgag aaagtcttgt ttttaacacg
    66421 agacgaagtg cctcattctg tcgcagtcgt tgttgattcg atgaagcgaa acgagaatga
    66481 taaaatccat atccaggcca cgatcatcgt cgaaagagat agccaaaaag gaatcattat
    66541 cggcaaaggc ggaaaaatgc tgaagcaagt aggaacgaaa gcaagaaagg atatcgaaaa
    66601 tcttttagga gacaaagtat ttctagaact ttgggtcaaa gtccaaaaag actggcgaga
    66661 caaaaaagtc tatctgcaag actttggcta ccgaaaagac gaatactgat attctgaact
    66721 tcttataggt taagtataga aataaactgg ataaaaatcc gcacgttgag aaaaacggcg
    66781 gaagatcgaa acataaataa tctggatcgg cagaatctgc ttttccagat ttatttttta
    66841 ggagggagaa tatgagccag cttgcagaat cgaaaggctt gattttatct agccgtaatt
    66901 acaaagaaaa agataaatta gtcaaaatct ttacggaatc ttctggcaaa atgatgtttt
    66961 atatcaaagg aatccatcgg caaaatcagc cattagctcc ggctttgctc ccctttaccg
    67021 aagcagtcta tatcggtaat tttcgaagtg aaggtttgtc gtttttaagc agtgtcaaag
    67081 acgtccatgc gtttcggaac atccaagaag atatcttttt gagcggttat ggtacctatt
    67141 tgctaaatct agtagatgca gcaatcgaag atcatcaata tgacccgcat ttattccaat
    67201 ttacgcaaca agctctgcag cggatggatc aaggagatga tgcagaaatc atcacgaata
    67261 tctttgaagt tcaaatactg caacgcttcg gaatcgcgcc aatttggacg cactgtgtcg
    67321 tatgcgggga aacaaaagga aaatttgact attcttccaa atctggcggt gtgatatgcg
    67381 agaaacactg gccaatggac gaacaccgtt atcatgcaga tcctcgcgct gtttatttcg
    67441 ttcgaatgtt ttcggctatt tcttatgata agatcagtgg aatccacttg aaagaagaaa
    67501 cgaagcaggc aatccgtgac ctcattgacc agttgtatga tgaatatgtc ggtattcatc
    67561 taaaaagcaa aaagttcatt gatcaaatga agtcttgggc acacgtatta aagacgccag
    67621 agaaaaatat gcctgaaaaa gagatacctg aaaaagaaga ataaatgaaa gatacaacaa
    67681 aaaaatcaaa actatttgac aaatacaggg acttccgtct attattaacc gtagattaaa
    67741 tagatcgttc actgagagga tctattatat aaacaagaaa actgcaaaat gaatacgtac
    67801 attgaccaag atcagtaaaa aacacgtggt ttagcgagtt cgggagagtg gaagccggac
    67861 ccaaacgatt tttgaagagg cacttgcgag tacgataaat gaagctgccg aatctttatt
    67921 cggaagtagg gtggaaccgc gaaattattc gtccctatgt ctgtaatcag acatagggac
    67981 tttttgtcgt tttctttcct gtattcttat tgacagcaac gcttaaaaat tgaaaggggc
    68041 aaaacagatg agtaaaaaat tgaccgtcca agatatgatt ttaacattac aaaaattttg
    68101 gtcatcaaac ggctgcatgc tgatgcaagc ttatgacaca gaaaaaggag caggtacgat
    68161 gagcccatat acattcttac gggcaatcgg accggaacca tggaatgctg cttatgtaga
    68221 gccatcccgc cgaccagctg acgggcgtta tggagaaaac ccaaaccgtt tgtatcagca
    68281 tcatcaattt caagtcgtaa tgaaaccttc acctgaaaat atccaagagc tttatttaga
    68341 aagcttgcgt cttttaggaa ttgatccatt agaacacgat attcgttttg ttgaagacaa
    68401 ctgggagaac ccatcgatgg ggtgtgccgg tgtgggttgg gaagtatggc ttgatgggat
    68461 ggagatcaca caattcacgt acttccagca agtcggagga ctaccttgta aaccagttac
    68521 ttctgagatc acttatggac tagaacgtct tgcttcatat attcaagaag ttgaaagtgt
    68581 ctatgatctg gaatgggctg atggtgtgaa atatggcgag atcttcaaac aaccagaata
    68641 tgaacattcc aaatattctt ttgaagtaag tgaccaagac ttgctactaa gcaactttga
    68701 ccgttttgaa aaagaagcta aacgctgtat cgatgaaaat cttgttcatc cagcgtatga
    68761 ttatattttg aaatgcagcc atacattcaa tttattagat gctcgcggtg cagtatccgt
    68821 aacagaacga gcaggttatc tttcccgtat ccgcaacatg gctcgtgctg tagccaaaat
    68881 ctttgtggca gaacgcgaaa aattaggatt ccctttattg aataaaaaca cagcaaatcc
    68941 agaaaacatg aaggaggctg aataagatgg caaaagactt attacttgaa atcgggctgg
    69001 aagaaatgcc tgctcatgtt gtcacaccaa gcatgaaaca attagaagaa aaaacagcaa
    69061 aatttttaga tgaaaaccaa ttgacttatg atacgatcga aacgttttct acaccgcgcc
    69121 gcttagcaat caaagtaaca aacattccag aacgtcaagc agacagtgaa gaagaagtca
    69181 aaggaccagc caaaaaaatt gctttggacg cagatggaaa ctggacgaaa gcagctgaag
    69241 gatttgtccg cggacaaggt ctgacgacag atgctatcac ttttagagaa ttaaaaggcg
    69301 tggaatatgt ctatgtaacg aaatacactc acggcaaaga agcgaaagaa gtattgacag
    69361 gtttagcaga tgtcattaaa agcctcactt tcccagtaac gatgcattgg gcagaatacg
    69421 attttgaata tatccgccct attcactgga tcgtggcatt gttagatgac gaagtaatcc
    69481 cgtttgagat cttagatgtc aaaacaggca gagcctctcg aggacatcgt ttcttaggcg
    69541 aagatgtgac atttgcgcat gcaaaagaat atgaagaaaa attgaatgaa caattcgtta
    69601 ttgcttcacc gcaaaaaaga caagaaatga tcgttacaca agcaaatgaa ttagccgaaa
    69661 aaaatcactg ggttattgat ctagacgaag atttactaga agaagtcaac aatttagtcg
    69721 aatatccaac tgtcttttct ggcgacttcg aagaaaaata tctttctgtt cctgaagaag
    69781 tactagtcac ttctatgaaa gaacatcaac gttatttcga ggtacggaac gatcaaggta
    69841 tgctgcttcc tcattttatt tctgctcgta atggtgatcg ggtaaaattg gaaaatgtgg
    69901 ttcgaggcaa tcaaaaagta ttgacggctc gtttagaaga tgcggaattc ttttataatg
    69961 aagacaagaa agcttcaatc gaccaatacg ttgaaaaact aaaaactgtt actttccatg
    70021 aaaaaatcgg tagtatctat gagaaaatgc agcgggtaca agtcattgcc cgtatcattg
    70081 gcgaacaagt aggactatct gaggaagaat tgatggatct gcaacgagct gctgaaatct
    70141 ataaatttga tctagtaacc aacatggtag gggaatttcc tgaactacaa gggatcatgg
    70201 gcgaaaaata tgctctgctg aaaggcgaga aacctgctgt agcaacagcg attcgtgagc
    70261 attacatgcc tatatccagt gaaggagagc tacctcagtc tacagtaggt tctgttttag
    70321 cgattgcaga taaattagat agcatttttt ctttctttgc agttgggatg attccaagcg
    70381 ggtcaaacga tccttatgcc ttacgtcgcc aagcatacgg tgtcgttcgg atcatcgaat
    70441 cgaaaaaatg ggcctttcct ttgtctgttc tgcaagaaac gattagtgat gttatcagta
    70501 aagatacgga tcgtttcggc attggtttat cagaagggca acaacaggtg atcgacttta
    70561 tcaaaggccg tctgcgccaa ttattgacaa cgaagaatat tcgtcatgat gtcatcgaag
    70621 ctgttttgaa tgcagaacaa aaagatctga caaaagtatt tgcagcagca caattgttca
    70681 agcagcatct tgcagatgaa gacttcaaac catcgatgga agctttgaca cgtgtcgtga
    70741 acttagcgaa aaaagcggag cttgaaaaac aatcagaagt agaccctgaa ctgttcgaaa
    70801 atgaagcgga gaaagaactg cacaaagcag tagaggcaat gaaaaaaagc tttgctgacc
    70861 aaacgatcaa cgaaaattat gatcagcttg taagccttcg cccactgatc gaaaattatt
    70921 ttgatcagac gatggtcatg gcagaagaac cagcagtacg taagaaccga ttaacgcaat
    70981 taagtcaaat tgctgccatg gctttgtcgc tagcaagttt agatcaaatc atcacaaaat
    71041 aatttctaat atgtaaataa accagagaat aggcacaaaa
//
LOCUS       DAJSBN010000008        64977 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJSBN010000008 DAJSBN010000000
VERSION     DAJSBN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.008"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            813..1199
                     /locus_tag="ONT86_000599"
     CDS             813..1199
                     /locus_tag="ONT86_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2351416.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1293..1484
                     /locus_tag="ONT86_000600"
     CDS             1293..1484
                     /locus_tag="ONT86_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2351417.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1998..2330
                     /locus_tag="ONT86_000601"
     CDS             1998..2330
                     /locus_tag="ONT86_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2351418.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2626..2781
                     /locus_tag="ONT86_000602"
     CDS             2626..2781
                     /locus_tag="ONT86_000602"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCR2351419.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3057..4397
                     /locus_tag="ONT86_000603"
                     /pseudo
     CDS             3057..4397
                     /locus_tag="ONT86_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4638..6254)
                     /locus_tag="ONT86_000604"
     CDS             complement(4638..6254)
                     /locus_tag="ONT86_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR2351420.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6372..6590
                     /locus_tag="ONT86_000605"
     CDS             6372..6590
                     /locus_tag="ONT86_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2351421.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6785..7246
                     /locus_tag="ONT86_000606"
     CDS             6785..7246
                     /locus_tag="ONT86_000606"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2351422.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7439..7678)
                     /locus_tag="ONT86_000607"
     CDS             complement(7439..7678)
                     /locus_tag="ONT86_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2351423.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7702..9225)
                     /locus_tag="ONT86_000608"
     CDS             complement(7702..9225)
                     /locus_tag="ONT86_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR2351424.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9243..9365)
                     /locus_tag="ONT86_000609"
     CDS             complement(9243..9365)
                     /locus_tag="ONT86_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR2351425.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9395..10378)
                     /locus_tag="ONT86_000610"
     CDS             complement(9395..10378)
                     /locus_tag="ONT86_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR2351426.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10402..10551)
                     /gene="rpmG"
                     /locus_tag="ONT86_000611"
     CDS             complement(10402..10551)
                     /gene="rpmG"
                     /locus_tag="ONT86_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR2351427.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10572..10949)
                     /locus_tag="ONT86_000612"
     CDS             complement(10572..10949)
                     /locus_tag="ONT86_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351428.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(10981..11160)
                     /gene="rpmF"
                     /locus_tag="ONT86_000613"
     CDS             complement(10981..11160)
                     /gene="rpmF"
                     /locus_tag="ONT86_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR2351429.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(11175..11444)
                     /gene="rpsN"
                     /locus_tag="ONT86_000614"
     CDS             complement(11175..11444)
                     /gene="rpsN"
                     /locus_tag="ONT86_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR2351430.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(11967..13733)
                     /locus_tag="ONT86_000615"
     CDS             complement(11967..13733)
                     /locus_tag="ONT86_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2351431.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13693..15447)
                     /locus_tag="ONT86_000616"
     CDS             complement(13693..15447)
                     /locus_tag="ONT86_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2351432.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(15557..16126)
                     /locus_tag="ONT86_000617"
     CDS             complement(15557..16126)
                     /locus_tag="ONT86_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCR2351433.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(16144..17559)
                     /locus_tag="ONT86_000618"
     CDS             complement(16144..17559)
                     /locus_tag="ONT86_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2351434.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(17570..18238)
                     /locus_tag="ONT86_000619"
     CDS             complement(17570..18238)
                     /locus_tag="ONT86_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR2351435.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(18368..18952)
                     /locus_tag="ONT86_000620"
     CDS             complement(18368..18952)
                     /locus_tag="ONT86_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2351436.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(19283..19516)
                     /locus_tag="ONT86_000621"
     CDS             complement(19283..19516)
                     /locus_tag="ONT86_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCR2351437.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(19531..20481)
                     /locus_tag="ONT86_000622"
     CDS             complement(19531..20481)
                     /locus_tag="ONT86_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR2351438.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(20478..21320)
                     /locus_tag="ONT86_000623"
     CDS             complement(20478..21320)
                     /locus_tag="ONT86_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR2351439.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(21322..22041)
                     /locus_tag="ONT86_000624"
     CDS             complement(21322..22041)
                     /locus_tag="ONT86_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR2351440.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            22733..22891
                     /locus_tag="ONT86_000625"
     CDS             22733..22891
                     /locus_tag="ONT86_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2351441.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            22963..24189
                     /locus_tag="ONT86_000626"
     CDS             22963..24189
                     /locus_tag="ONT86_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2351442.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(24277..24669)
                     /gene="rpsI"
                     /locus_tag="ONT86_000627"
     CDS             complement(24277..24669)
                     /gene="rpsI"
                     /locus_tag="ONT86_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR2351443.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(24685..25128)
                     /gene="rplM"
                     /locus_tag="ONT86_000628"
     CDS             complement(24685..25128)
                     /gene="rplM"
                     /locus_tag="ONT86_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR2351444.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(25647..26114)
                     /locus_tag="ONT86_000629"
     CDS             complement(25647..26114)
                     /locus_tag="ONT86_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCR2351445.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(26234..27772)
                     /locus_tag="ONT86_000630"
     CDS             complement(26234..27772)
                     /locus_tag="ONT86_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR2351446.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            27852..28538
                     /locus_tag="ONT86_000631"
     CDS             27852..28538
                     /locus_tag="ONT86_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR2351447.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(28739..29266)
                     /gene="msrA"
                     /locus_tag="ONT86_000632"
     CDS             complement(28739..29266)
                     /gene="msrA"
                     /locus_tag="ONT86_000632"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR2351448.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(29350..30027)
                     /gene="pgmB"
                     /locus_tag="ONT86_000633"
     CDS             complement(29350..30027)
                     /gene="pgmB"
                     /locus_tag="ONT86_000633"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR2351449.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(30024..31154)
                     /locus_tag="ONT86_000634"
     CDS             complement(30024..31154)
                     /locus_tag="ONT86_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR2351450.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(31154..33337)
                     /locus_tag="ONT86_000635"
     CDS             complement(31154..33337)
                     /locus_tag="ONT86_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR2351451.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(33334..34359)
                     /locus_tag="ONT86_000636"
     CDS             complement(33334..34359)
                     /locus_tag="ONT86_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2351452.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(34404..35231)
                     /locus_tag="ONT86_000637"
     CDS             complement(34404..35231)
                     /locus_tag="ONT86_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR2351453.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(35228..36295)
                     /locus_tag="ONT86_000638"
     CDS             complement(35228..36295)
                     /locus_tag="ONT86_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR2351454.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(36321..37157)
                     /locus_tag="ONT86_000639"
     CDS             complement(36321..37157)
                     /locus_tag="ONT86_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2351455.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(37154..38023)
                     /locus_tag="ONT86_000640"
     CDS             complement(37154..38023)
                     /locus_tag="ONT86_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2351456.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(38093..39376)
                     /locus_tag="ONT86_000641"
     CDS             complement(38093..39376)
                     /locus_tag="ONT86_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR2351457.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(39390..41075)
                     /locus_tag="ONT86_000642"
     CDS             complement(39390..41075)
                     /locus_tag="ONT86_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR2351458.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            41350..42375
                     /locus_tag="ONT86_000643"
     CDS             41350..42375
                     /locus_tag="ONT86_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2351459.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(42970..46644)
                     /gene="rpoC"
                     /locus_tag="ONT86_000644"
     CDS             complement(42970..46644)
                     /gene="rpoC"
                     /locus_tag="ONT86_000644"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR2351460.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(46702..50319)
                     /gene="rpoB"
                     /locus_tag="ONT86_000645"
     CDS             complement(46702..50319)
                     /gene="rpoB"
                     /locus_tag="ONT86_000645"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR2351461.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            51047..52015
                     /locus_tag="ONT86_000646"
     CDS             51047..52015
                     /locus_tag="ONT86_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2351462.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            52033..52938
                     /locus_tag="ONT86_000647"
     CDS             52033..52938
                     /locus_tag="ONT86_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2351463.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            52935..53741
                     /locus_tag="ONT86_000648"
     CDS             52935..53741
                     /locus_tag="ONT86_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2351464.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            53877..54866
                     /locus_tag="ONT86_000649"
     CDS             53877..54866
                     /locus_tag="ONT86_000649"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCR2351465.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(55183..55707)
                     /locus_tag="ONT86_000650"
     CDS             complement(55183..55707)
                     /locus_tag="ONT86_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCR2351466.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            55994..57805
                     /gene="scm"
                     /locus_tag="ONT86_000651"
     CDS             55994..57805
                     /gene="scm"
                     /locus_tag="ONT86_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCR2351467.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(57960..58946)
                     /locus_tag="ONT86_000652"
     CDS             complement(57960..58946)
                     /locus_tag="ONT86_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR2351468.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(58969..59535)
                     /locus_tag="ONT86_000653"
     CDS             complement(58969..59535)
                     /locus_tag="ONT86_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCR2351469.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(59549..59965)
                     /locus_tag="ONT86_000654"
     CDS             complement(59549..59965)
                     /locus_tag="ONT86_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR2351470.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGNYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(60422..62356)
                     /locus_tag="ONT86_000655"
     CDS             complement(60422..62356)
                     /locus_tag="ONT86_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2351471.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            62637..63593
                     /locus_tag="ONT86_000656"
     CDS             62637..63593
                     /locus_tag="ONT86_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCR2351472.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            63683..64786
                     /locus_tag="ONT86_000657"
     CDS             63683..64786
                     /locus_tag="ONT86_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR2351473.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            64799..>64977
                     /locus_tag="ONT86_000658"
     CDS             64799..>64977
                     /locus_tag="ONT86_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR2351474.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
BASE COUNT        19784 a        13176 c        11204 g        20813 t
ORIGIN      
        1 taagtagttc tatctgagcg atactctttt ataatactga attcggaatt aaaaaccacg
       61 ataggtaact ttaaacaaac atgaagagac tttaataaga cttctttatc tgaaaacact
      121 tttgtttctc tctttccttt ctacatcatt aaatagtatt ttatacgtta tctaaaatac
      181 tattcctctg tcaaaataag ctaacttact tcattataaa atatccactt atcgctttca
      241 tgtatattcc accgtaaaaa gtacatttta tcaccgagaa ctcatcattt ttcatcatga
      301 tgaatactat ttacatatat ctagttcaaa gacaataatt attatgatcc gttattatct
      361 aagcactata cagataatag gaatttttcg tttctaaata ttccaatttg ggaatccaat
      421 aaaaattttc gttcaaatac gtgtctattc gaactctttt ctagttgtta attatgaagg
      481 gggaagaaat aaaaaataca atctatacac gaataagaat attatccaaa aggaggtttt
      541 agaaaagatt tattatacgt aaaaagctca agacattttc ttagccataa ggacatgttt
      601 gatatttaag aagatatatg aattttcttt tctaagtata aagataacta tccattatca
      661 actaaaaaaa catttttgaa aataaacatc agcagtccta tcaatgcatg tgagcttata
      721 gaagttattc gcagcgcagc ataattgcat tagcttcttt tcgtgttttt tgcctctttt
      781 ccctttaaat cttaatggag gaggtgtgca atatgtcaga taacgacgaa cgcgtaaatg
      841 cccagttgat tcgattcttt gaaaaggtca ttttgaacac cgctaatact tattataaaa
      901 aacaagaaga aattcaaaaa catgaacaac ttgatcaact cccaccatat ttgtttacta
      961 aagatactat aaaatttaaa caaccgatta atgttttaaa tgtgactctc cttgttgaag
     1021 atataaaatt agcaaaaatc cttcctttgt tgaaagaacc tgaacaaatt tttcttatcg
     1081 aaaaatttat ctttgataaa actgataaag aaattggaaa ggttctgggg attacaagac
     1141 agggtgcgac aaatttaaaa catcgacttt acaaaaaaat tcaaaaaatg ctggtttaat
     1201 atctgcataa taactaattt tgtatgcttc tttgctcgtt ctcctccttc ccaaagaaat
     1261 agattataag tccatgggaa gaaggtagat ttatgaattc tttaaaagaa gatttcattt
     1321 tagcaaaggc tggaaatgaa gaagctgttg aggcaattct caaaagattt agctctttga
     1381 ttcataagca atcctggaga actggaaaat atgatcaaga ttgttatcaa gaatgtatgc
     1441 ttgcaatata tctagctatc tcgaaattcg aaattaaaga ataactcaac taagggagtc
     1501 tgtgacataa gtaaaaagat agtttcaaat ttcgaacaat aaatttgaaa ctatcttctt
     1561 tataattata aagtatcgat tttacagcgt tctataatga ttcatagaat ctaaaacgaa
     1621 tgattttaat tatcaacgtt ttttaattcg tttttagctt aaaaataact tttgtcacag
     1681 tctcactcct aaaattactt tatttacgta tgaagtgggc aggaggtcga atccagaact
     1741 gggctatggt acttcaacga ggacttctcc tctagaattg aaaagcatgc gatttacctt
     1801 ccgtaacgac ggtagattta ttcaactatg tgtccaatca aaatttgtct gatttcaaaa
     1861 atagtcaaga gaattttggt tgttaaaaac tcttatctat caacttccac agtttagttg
     1921 acaaaccctg atactaagtt gggctatgat acacttttct tgactatcag ttacgttacc
     1981 ggaaggaatg aactctattg agaaaaacat atgatcgtaa atttaaacta aagatttcac
     2041 aggatatcct tgagaaaaaa gtaactacca agaaaattgc tgaagaatac aatatctctc
     2101 gtccaacaat ttcccgatgg gtttcagaat atcgtcgata cgggaaaaat gcttttgctg
     2161 gacaaggaaa aagattacct gataaagcag atttctacat tcttgaacag gaaaataaac
     2221 gactaaaaga ggaagacgag tgttctacga acacaaagat cgatacggat ctccagggat
     2281 cactcaaaaa ctttacgatg aaggaatcga aatcaataaa cgtgttgtag ctgttcttat
     2341 gcgtgaaatg aatttatgca caagaggatt tcatcgacgc aaatcttctc atggtagaga
     2401 aaaaacaatc aaagaaatgg taaaaaaatg ttgaatcgat aatttgaaca gaaccaaatt
     2461 gatgctgttt gggtgatgga tattacttac attccatgta atgacggtcg cctctatttg
     2521 tccacctata ttgacttagc aactcgtatt ctacgttact atatggtaga ttcacacatg
     2581 aaaaaagaga ttgtcgtccg tccattacaa atttattaag gagaattgcc tacggtaatt
     2641 cattccgata gaggtagcca gcatcggtct tttatttatc aagagctgtt gaacgaccac
     2701 cacatcacac attcagtgag ccaaactggg actcctgttg acaatgcagt aattgaagcg
     2761 tttcatcgat caatcaaatg agaattaatc gactccaaca aatataaaag taaaatagag
     2821 atgaaggtgt taatccaaga ttatttagaa gactactacc caaacaaaag aatccataca
     2881 aaatttatga tgactccttg acaatttgaa gcgaagaaaa taagggatct ataatatcta
     2941 gtgttggtga cgccttctgt tgccaatact ttttcttttt tctaataaac gcaaacagac
     3001 accttaaaat cctttagaat aaagtcgacg aaaaccattc aaaggagtgt ttcaagatgt
     3061 cctattcaga gtttaccaaa gagttactag atattctaga cctaaatctt atctttcatg
     3121 aagatgcctt tcgcaaagag cgaatcaatg acgaaacatg ttttgttttt gatggcacat
     3181 tgacctatca accggaggaa tgctttcatc gccactatca aaatgaacaa acgatcatta
     3241 aatagggctg gaaaaaagta tccatcttat tgaatgatgt cagtaactac aagaccattc
     3301 tgcggattaa caaacagcgc ttcaaatgta aacactgtgg aaagactttt ttagcagaag
     3361 attcagttag tgatcgtcgc tgctccattg ctcgcagagt caaacaagcc attcttgagc
     3421 tattaagcga acctctttct atgtctttga tcgcacgtat gaagcacatt tctccgacca
     3481 ctgtcattcg aatacttcgc agccttcggc ctaaaaccat ttctttgagc cagctgttac
     3541 cagaagtggt gtgtttcgat gaattcaaat cagtgaaaaa tgtctcggga gccatgagct
     3601 ttgtcatgat ggacggcaag acccatcaac tgattgatat tgtggaaaat cggcaactca
     3661 atcctttacg cgactatttc ttgcgctacc ctataaaagt ccgtgaacaa gtgcgtctag
     3721 tggtttcaga tttctataca ccctatcgca cattggtgaa agagtgtttt ccgaatgccc
     3781 gaatcgtcgc cgatcgcttt catattagcc aacacattgg tcgggctttt accaaccacc
     3841 gaattcaagt aatgaagacc tttaaaaaag gggatcgtcg gtcgaagcac ttaaagaaat
     3901 actggaaact acttcaaaag aatgcgtggg aattgaaagg tcagcatcgc tactggcgtc
     3961 cgtcatttcg agatcatctg acagaagcag agatagtcga tcggctatta tcttatgatg
     4021 attccttaaa gcgaggctac gaagtctacc aggattttct ttccgttatc agaagacaag
     4081 atgttcctga attcgtcgcg ctcctaaaag aagactacaa ggagttgcca gagcactacc
     4141 aaccagtgtt cacgaccttc aagaaatacc aaacagaaat caaacgagcg ttgcgcgttc
     4201 cctattccaa cggccctata gaatgtttga ataaccatat caaagtacta aaacgaattg
     4261 cctatggcta tagaaacttt caaaactacc gagaacgaat ctttttatat cgaggaaaat
     4321 attttaagaa aactaagaat actacccaac tgactaaagc cagaacaaca acacggctag
     4381 acaagatagc tgtctagccg tgtgagtatc tcgattattc tattgaattt cttcaccaac
     4441 actcattgac gaagagccaa aataagtaat ctaaccgact gttaactaaa ccattgcagg
     4501 tcccgttaaa gatacttgtg cctgttgtca ccgcttcttt ttcttgtcca caagcgctta
     4561 acacacttaa taaagcccct gctattatta ataataatat ctttttctta ttaaaaacct
     4621 cctcatctat ttccagatta gtaaatgtag taatctgtgc tttctttttt ctcaatacta
     4681 ataactaact tatggggtaa acaaatactc gtttgaccgg gttttgagat ccatccagtt
     4741 ctaaccgcga tttgatcagg actattatct tctttaactc gaattcttcc atctttaatc
     4801 tcaacaatat tatattgtcc cttcgcagga tgataattca cgagtttatg attgatctta
     4861 tccaaatcga agcgatccat ttccttgcca tcaattcgaa cgatggcata tttgatagca
     4921 tcttcagttg tttcattctc cttttctata gagaatacaa ataagggaat aaaagaggta
     4981 ataatcaaaa tgccaataat tatgacatcg cctatcttga caccagcttt tcttaaaaat
     5041 ttcattaacg atatctggct aaacatatta gactttttcc atggaaatgg tgaaattttt
     5101 cggaaattat ctgcattaag agaatcagtt aaacaaccct caagttgttc cttaatacat
     5161 tgttgatcca tcttcatacc aataaaaact agtttattaa tgcgttcacc aaacaacgga
     5221 tgccaggctt ccttcaattc gggatttttt tgtataatca tttgacactc aattttaggt
     5281 aaactagcta cccaataatt taatgattca atacttttat agctacccga tgtttcaaag
     5341 atcgtcgaaa aatcactatg ttgggcaaac caaagaaacc cttttgctct tgtaatattg
     5401 ccaggaaaat ttttatccgt aagatgggca aatttttgtg cgtcaaggag taaccgattt
     5461 cgatagataa aacttgagat accatattct tctgtttcag gaatatgatt ttgatagcca
     5521 agctctaact ctttgaacca gcccactgaa taggaagtct catctatatt aaaaagattt
     5581 gtatttagaa ttttcgataa gtctacttca cctttaattg tcctaattat tttggcattg
     5641 gactgtaact tagcaattag accgttaatt ttgtaaatcg tcttggttga cactaagtca
     5701 ctattattta aaagtaatat gttacaacac tcgaattgat caattaacaa gccacgaata
     5761 tcctgttctt cttcaactac attaccttca gcttttcttt cctgaatctt ttcacccgtt
     5821 tcgaaattat cccaaaaacg ttttacatcc actactataa taatggcacc aatctctaat
     5881 cgttgggtca aatcaatgtc taaagggttt ttagctaata taattgcttg aataattgga
     5941 attggtttgc tgattcctga agcttcaata acaatttgat tcaagtcaga aaattgcact
     6001 aattgatcta gttcaacaat caaatcttcg cgtaatgtac aacaaatgca tccattcggc
     6061 atcgaaacaa gtttttctcc agttctttta aaacctttct tgtcgactaa taccccatca
     6121 acatttactt cccctatgtc atttacaatg acagcaattt cctttccgtg atcaccgtac
     6181 agaatatgat ttagaagtat cgtttttcct gaaccaagat agccagttaa gattgtaata
     6241 ggtatttttt tcatagggaa cctctttcta taccttgaat ttacacttta agtgcaacac
     6301 ataaaaatga gtcataatcg atatccaact agtataagtt tgtcgagaac actcctagaa
     6361 ggaaaataat tatgagccag tatcgtcatc ttactatact cgaacgagaa agaatctttc
     6421 tgcttcacga agaaggattt tctattagta taattggcaa cgacattcag catagcccgt
     6481 ttactatttt acatgaactg agaagaaaca agaaaaaaac tattcacctt ccaaagcgga
     6541 aaaaaattat gcttatcgaa gaaaaaaatg cggacgttat cacattctaa atgatccgtg
     6601 agtttggtca attgttcgtc atttatttgt cgattttcag tggtcgccag aggaaatctc
     6661 gaatcgtttg aagcatgaac atatggactt ttcaattagt tttatcacga tctaacgtgc
     6721 aatttgcaat gatcttttcg atacagaacc cctctcacac ggcaataaag gcctgatccg
     6781 ttcattgcac catcgaggaa aaacaagaca tacaaaaaat catgtcgagt ctcgtggaaa
     6841 gatttgcctt actcattcga tccatggccg tccaaaatcc gcagatgata gaagtgattt
     6901 tggtcattgg aaagccgata ttgtagctgg caaaactggt ggtacttgtt tagtgacttt
     6961 gacagacaga aaatcatgct tccttttagc aggaaagaag gtccccagaa agaaagccgc
     7021 ttatgtgagc gagaaaatga ttgaactttt aagcagtatt ccaaatgagg cggtcaaaac
     7081 gatcactcca gatagaggaa aagaattctc caatcatttc gcagttacag aagcattgaa
     7141 taatatgccc ttttattttc cagatcctca tgcccttggc aacaatgaac aaattaaaat
     7201 acgaatagac tcattcgaga atacttgcca aaatcgaaag agatagctac ggtagataac
     7261 tcaacgataa atcagatgat tgaaaaactg aataacagac ctagaaagtg tttagattgg
     7321 agaacacctt atgaagtctt ctttgataaa gtgttgcgct tgatttgaca attcaagaca
     7381 taataaaaag cagatcaaat ccaaataagg gaaaaatgat ctactctggt attatcaatt
     7441 aattggccaa gctattatgc cgttttgcta aacgtctact tcttccagta ttgaccttga
     7501 taaaacaaca atcaggatcc cgttttaata ctctatgaac tgtgtcaata ctaacatgta
     7561 aagttgcagc aatatcagct aatgtactcc catttaatct catttgcttt gcaaatcgat
     7621 aacgccgata agcatcaata ttcttggaat ccatagacaa taatactttt cttaccatgt
     7681 atttcctcca aaaattgaat tttaatgagc catcatttct tgagcaattt catgtttaga
     7741 caatgaagct ggatagaaag taggccaatt atgcaattca ttatagagtt tttcttgact
     7801 ttctccacca aagaaaatat ggaaatgctc tgccttactt ggtgcaattc catgatcact
     7861 aaattgaata tatttaaaac gaccagcacc tgcatcatca gtttcaaaat taaaacgaac
     7921 accacggttt cctttttcat aattcaaaat tttgtatcca acatatttgt atgtgtattg
     7981 atgatgttca ccgttgacta caaaatcaat ggtgttgtct ttaatgttga tagtatcaat
     8041 atctgtctta tagccagtag tataataatc tttgtactcg gcagctgtca tgtctttatt
     8101 gatttttgct ttgtaatcga aaacttcatc caaaattcca tcttttaaca acggataaac
     8161 agattgccat tcgcctgtat aatcactcaa ttcgcgatcc ttcacatcgg catctttaaa
     8221 gtacccatta gcaactgtct tttcatcctt cgcttctttt tctggaacta cctcttcacc
     8281 agcaaccata gttgtttttt ctaacgcttt tagattgtct tccattactg aaacataatc
     8341 ttcaccatta tccatttgtt cttttgtaag actttcaaga ggattcagta cttccaattt
     8401 tatcccagct tcgttggcca aagtttttgc aattttatca ttagcattct tttcaaagta
     8461 aatataattg atcttatttt ttttaacata ctcttttaat tcagctaatc gaccagaaga
     8521 tggctcttct tcaggactta gtccagcaat aggaacttgt ttcaatccat aatccaaagc
     8581 taaatatcca aaggctgcgt gttgcgtcac aaaacttttt tgtttcgctt ctttaagact
     8641 agtggtatag tcagcatcca accgtttcag tttagtcaaa tacttttcag catttgtctc
     8701 aaatactttt gcttttttcg gatacaattt aactaactga tccttgatat ttgaaacttc
     8761 cttaattgcc attttgggtg aaacccacgt atgaggatct agttcatgat ggtgatcttc
     8821 ttctccgtga tcatgatctt cttcttcact acctggcaat aaaatcatgt cttttgttcc
     8881 ttctactaca ttaggcccag ctttcttcca actttcttta gcttttggta cccatgattc
     8941 catgttttcg ttatgatata caaaaacatc agcatcatga gcttttgcca tatccttagc
     9001 tgagggctca tagtcatgag gttctgatcc cgctggaacc attaaatcca cgttcgcttc
     9061 atcgccgacg atgtttttgg tgaaatcata cattggatag aatgttgtta caatttctaa
     9121 ttttccatct ttatcattcg atacttttgc atcattagtg ctggagcaag ctcccaataa
     9181 tcctagagct gctatcccaa taagtcctac actgattttc ttcattcttt ttccttcctt
     9241 aattatgcta atttcttcat tttatttcgt ttatgttctt tgataatttt taatgccctt
     9301 tttctagttt taatattcgg gcttttaagt cgtcgatatg cactagatat atccgttttc
     9361 tccattagaa attaccccca atcactactt atcattattt gctttccgcc agtaatttat
     9421 caaaactctg tttgatcctt tcttcatcca acatttttcc gattagaata atagtggttt
     9481 ggtctttttg tcgagtggtg tattgaaaat taacctgttc gtttacctct tgcatggcaa
     9541 ctataaaatc ctgctcttgt aaagcaatca atcctttaaa acgatacagg ctcccttggt
     9601 tggttaaaat cagccagtca atccattgga gtaaaaaatc tttttttatg tcttgatctg
     9661 aagttaagag aattgattta aactcatgta cttgatggtg ttgatgatag tatttgccat
     9721 tatagtgatt ttctgatgaa tcactttcaa aaactaaggg atgattaaat tttttcaaat
     9781 tataaaaatc ctttttttga atcgtttcac tatgtgtgaa cattcgaata tcggataaag
     9841 ggttaatact tttgatttct ctctgtaatt tttttagtgc agataaattc aagttttgtt
     9901 ttctagacac aaaaatgcga tctgccatga caagttgctt aagggcttgt ggttctcttt
     9961 caataagttc aaaactgtca ctatcaataa cagtcaataa actatcgata tatactcttc
    10021 ctcttagttg aggcgctgtt aaaagtgttt gaatgattgg ttggggatct gctatacctg
    10081 tagtttcaat gatgatttgt gaaattggtt gttgatgttc ttctattgtt tccagtaaag
    10141 aaatcaatat tgctaataaa tcactgcgaa gactgcaaca catacatcca ttatttagtt
    10201 gtaacacccg ttcctcgcta tgaactaata atttgtgatc aatcccaact tcaccaaact
    10261 cattttcaat tataataatt tcttccctgg ggtagggtga gacttgcaag gcttgattga
    10321 ttagcgttgt ttttccagcc cctaaaaacc cagaaacgat tgtaactggt atgcccattt
    10381 tgtcaccttt ttaccactta gctatttcac ttctttaaaa attgcccgtc tgcgcaattt
    10441 tggcgaatac ttttttaatt ctaaccgttc agggttattc cgtttatttt tgctggtgag
    10501 atacagacgt tctccagttt cgacacattc caaaataata ttttgacgca taaatcttcc
    10561 tccttcaata cttatcgatc ttcaagatat gcttgaattt gttgtgcatc ttcaaaagtt
    10621 aactcgccag attccaattg tgaaactaaa ttttctttca tatcaacagg tatctcccaa
    10681 taaccatcga tacattgaaa taactcctcg tcatactctt caaaaattgg atagattctt
    10741 aaaaaatcat caaatgataa gccttggtcc tcatagtaat ctttgactaa tccattttca
    10801 attgcctgtt gggtcggtaa acccgtttca gtaataattc ctctctctac catcgaacga
    10861 taaaaagtgc atttatctag cgattgctgg gtcgtttcaa gataatactt taatccttct
    10921 tcaactaaag gtgggtaaat aatttccatt ttgattccct cctaaaagtt atatattaaa
    10981 ctattttgaa gctcctttaa ttactttctt tccattatag taccctttta aagatacatg
    11041 atggctacgt ctataatcac cattcgcttc gtcaaatgaa atttctggtt ttactacagt
    11101 ttgatgagtt cttcttaacc gtttcttcgc cttagatgtt tttcttgctg gtactgccat
    11161 gatatttcct ccttttacca acttgctttt tttactccag gaatcaaccc ttgatgggca
    11221 agttctctaa acttaatacg ggacataccg aactttcgca tataccctct tggacgacca
    11281 tccgtctgat cacgaagcct cagacgattc ggatttgaat ctcttggcaa tttagcgaga
    11341 gcggtgcgat ctcctgcggc ttttaattcc tgacgtttat ctgcatactt ttcaatcatc
    11401 gccatttgtt tctttgcttt agcaattttc gacttttttg ccatatacct ttatactcct
    11461 tttattttga tttagacacc tcaaatcgta atcgttacgt tttgacactg atactttacc
    11521 aacaagagtt gctcttgtca acaataaata acatgaaata aaactcatat gaatagataa
    11581 ctatttgaaa acgaatttat cggtacatag agataatcaa atcatttaaa agaatatgca
    11641 gtttaggctc tgaaaaataa gaattcactt ttattagtcc aatcaaacac aaataccgtt
    11701 ttaagtagct tttcctctac ttaaaacggc aatatgaaag aaactataac tttttattca
    11761 atcttttatg tttaactggt ttatcgccaa ttttttaaaa ctttgattat tccttatatt
    11821 ataagaatag acattaagtg aactaattca atcatactga ttctttcgtg tattcaaaat
    11881 tagatgctgt ttaataaaat tattcttttt gaaaaagaat tataactata tcccaaaaat
    11941 tgtaggttaa tttaaagcct atttcatcat cgtgattgag cttctttttc atagacgaat
    12001 ttctcagata gtttagtttt ctttatgaga tttttgtata tttctgacct tcgatacaac
    12061 tcattatgag taccttgatc aactagtttt ccttgatcca tcactacaat tttatttacc
    12121 ttttcaatag acttcaagcg atgcgatata attagtactg tcttatcttg tattaaacga
    12181 tttaaacttt cctgaatacg tttttcattt tccgcatcta aactggcagt aatttcatct
    12241 aaaattaaaa ttggcgcatt tttcaaaaaa gcccgtgcta tggataggcg ttgtcgctct
    12301 cctccagaaa gtgaacttcc gttttctccg accaatgtat gataaccgtc aggtagtttt
    12361 tcaataaagt cttcgcagtt agctaaacga gctgcttttt tcacttcctc atctgacgct
    12421 tgactatttc caatacgtat attttccaaa atacttgtat taaacaaagt tacctcttga
    12481 aaaacaatag cgattttgct aaacaaagaa gctggagata cacgtttaat atcataccca
    12541 tcaattaaaa tacagccttc atcataatca tacagtctag acactaattt caagatacta
    12601 gttttcccgg aaccactagc acctactaac gcagtcactt ctccttgttt agccgtaaaa
    12661 gagatatgat ctaaaattgg ggtattgtta tcatatgaga aagagacatc tcgcaactca
    12721 atgtcaaaag atttcagagg actatctgat ccctcttgga tactggtttc cttcatagct
    12781 ctaattcgct gtattttagg agctaaataa aatatttcaa gcactgcttc tttcatagaa
    12841 tcgaaggaat cctttatttt tattgcggct agtaagtaac caacgacgta gagtatagtc
    12901 acttcacccg ttaacagtaa gtgaacacct actagaatga ctactgctaa actaacatat
    12961 gagaaaatag aggataaggc catgactgaa aaagtactta attctacctt taaatgaatc
    13021 ctttcactct cttccatttt tttgaaaagt ctgtcttgaa cttttttaga tagattaaag
    13081 ctattaatct cttgctgcaa ttctatagtt tcttgaaatt cttccgagtt ttctctcaaa
    13141 gtatcgtaat atttagtatt ggctttcgtt tgggatttct ttgataacaa gattaagaca
    13201 aaactaaata acgttggcaa aataacagct aatcccattt tgacattacc aaccaaaagc
    13261 atcactgaaa taaaagggaa aaacagagcc ataccgccgg ctttaggtat tgcatgactc
    13321 atcgcatgct caataccttc aacgtcagac atgatagttt gagataaatc tgataaatta
    13381 tgttttgaaa aataggataa tggtaaattt gacaaatcgt ctgcaatttg cactcttaaa
    13441 tgcgcacttt ctttataagt actattatat aagttttcat actccctatc taacaaccaa
    13501 tacatgaaaa gcaaagtaac aaacgatacg gcaaaataaa gccaacgggg ctttgtattt
    13561 tctaaaacta attcgtctat cagcatcatt ataatgaatg ctggtaccat attaatgcag
    13621 tatacaaaaa aactagaaat agaagcttta gttaaagctt ctgcgccctg atcacttaaa
    13681 gcaaatttgt ttttataaaa acttttcatt gtcaagcctc caatcatttg cattctcata
    13741 attagtgacc aattgtttat acacttgatg tttagccatt aattcattat gatttccacg
    13801 ttctactatt ttcccttctg acaagacaag aatttcatct acagatttaa tgctggataa
    13861 tcgatgagct atcataataa ctgttttttc tttcatcaga tgttgaaaag ccttttgtag
    13921 ttcatactca ttatctggat caatagatgc actggcttca tccataataa caatctttgc
    13981 atcttttaaa attgctctgg caatagctat acgttgtttt tctcctcctg acagataaac
    14041 tcctttacta ccaattaagg tatgctctct ttcgggaaat ttttctaaaa tcgattcgca
    14101 accggctaat cttagcgcgt caaatacttt acttgctggc gcttgaggat ttgctataga
    14161 aacgttatcg taaatactct gattgaatag tttcgtatct tgaaagacaa aagcaattgt
    14221 gttaataatt gtgtcttttg aatagtgatt taaaggctta ccaccaattt taatctgtcc
    14281 cttatccaca ttgtagaaac ctgaaattag tttagctatc gtactctttc cgcttcctga
    14341 ttttcctact aatgcataaa ttcttttttg ttctaaaact aagttgaaat tttctaaaac
    14401 acactcttct ttgtatgaaa agctgacatt ttcaaatgca atgtcacaat ttttaatctg
    14461 tgtctcgttc ccatatttta gttggtcttg cttcatttct aaataaaggg agtctagtgt
    14521 ttcgactgca tatcttgctt caaacaagtg catacttaca tacattattc tcatgaaaga
    14581 aacaaaaata attccactta aaaaaaacaa catcaacaag tcaacagcta atatcttggg
    14641 agtggtaaga gaagttataa aaaaggttat aggaactatc aaaatgcaaa ctatgccaaa
    14701 aaatagccat tgaaaaagaa caaaaggtgt tttcccactc aaagaatact tatatgcaaa
    14761 cttagcataa tcgttaattg ctttattcaa ggctttaaat gatgttactt tagcaccaaa
    14821 tatctttata acttgaattc cgcggatata ttctaccgtt tcagagctta acacatctaa
    14881 cgattcttga tatttttgaa taaaatttgt attctctgtc attttcttta acaagaatac
    14941 accaattact aacatggcga ttagaaccaa tccaatttta aagttaatga aaaagcctaa
    15001 ggcaactgat aaaacaggtg ttactatggc ttgagcacta tctggaatca agtgagcaat
    15061 tgccatatgt gttttagccg cgttgtcatc aattgtcttt cgaatatatc ccgaagaatt
    15121 taaatcaaaa aaacgaaaac ttgcttctgt taaaccgcta attccctttt ttctcaaatt
    15181 agtctctaag cgaaacccaa ggatatgaga aaataagcca gaaaaaatat aaaataaagc
    15241 tcctaaagtt aaccaagcaa cagtttgaac cgcataactc ttagctaaat attcatctcc
    15301 tacaataatt agagactgaa gaaatttgta aatactgtaa tatccataga ctgttaatac
    15361 gacagaaaaa tcagaaaata aagtggctaa ccatccgtag tatttcattt tagggacata
    15421 actaaataat tttttgtata ctttcatttt ttcctccccc gcattcaatt tcttctctta
    15481 ttcgtttaca actgttaatg ctttccgtaa tagcgtaaat tagagatgta caaacagata
    15541 aaaggtaaac tacgttctat tttttgtatt tttttaagac ttttcttcca atcaatacac
    15601 caatcataga taaacttgct acaactaaca ctgaaatgat tccccatata cctaccgcat
    15661 agtggatcat tgcatccatt gttgcagcag attgtccttt atctatccaa tcttgacgaa
    15721 aacgatctgt aaagaaccat ataggaatcc actgtccggc agcactgcct aattgcatta
    15781 gagcccaaga cgcaacttga ggaattgctc gatcatatcc aaatacagat agaataatat
    15841 cagctaagat cgccatacta atcgaactga taaaccaagg taagtacgcc ccgcccatta
    15901 acgtaaaaaa taacaataaa acaattgtgt aaatagtaaa aattcctttt ctagggtagt
    15961 tatagctaat ataaacaaat atgattccag ataataatcc aaaaatcccg ggggatatgc
    16021 tgtgaccaaa cgccccacca atactactaa ccattattga aaaaaagtaa attactaggg
    16081 aaataactgt agctaaacca ataaaaatat aatcttttgt tttcatatat attctcctca
    16141 attctatttc gttatgtcat taaaaatctg ttctatcaaa gttgaatctt ctactctaag
    16201 ttttatatct ttttttattc caccattttt taaataaata attctattgc atgttttata
    16261 aagaaattcc ggatcatgag tagctataac tactgcaccc ttttctttta agtctttaat
    16321 aaggtcagca atcctttcca tattaatata atccaaccca cttgtaggtt catcaaatag
    16381 aaataaatta gtttttgaca atgaagctat agaaaccaat aatctttgtt tttctccacc
    16441 ggataaatca aacggtaatt gatctttcat tttataaagt tccatttttt ttaaaatttt
    16501 ttcttgctga tctttactaa ctatttcatt ccccagggct aactcgctcc tcactgattc
    16561 tgtgaaaaat tggtaatcca tttcttgcat tactaaaaac ggcgatgaca aatttttttt
    16621 attttcgatt atctttcctt tggtagggcg aataatcttc atgagtattc ttagtaaggt
    16681 tgttttcccc acaccgtttt tccctaccaa accaataaca tctccaactt tgacatcaaa
    16741 atttatgtct tttagaatat tttcataagt aatttcttca acttttaata catctgcact
    16801 taccttttcg atatagggaa cttcaatcga actattaaaa atgtcaaatt tccttaatgc
    16861 gttattgttt tcagttttta acaactgaat agttgtagtt tttacagttc tatttttaac
    16921 aaatagaact tgatcaatta attctttcaa atagtaaaaa cgatgttcag ctataacaat
    16981 agtaaatcct ttatttttta aatcacaaag taagtcagca agtttcttag tcgctttgaa
    17041 gtccaaattt gaagacggtt catccaatat aattaattta gagtttagcg ataaactaca
    17101 tccaatagaa attttctgcc gttcaccact tgatagcgaa aaaatgtttt tatctaatat
    17161 atttgaaaga tctaataatt cagctaattc attgattctt ttatccatat gctcttttgt
    17221 aaatccaaag ttttccatcg aaaatactaa ctcactcgtt gtatttgttg taaagaattg
    17281 tcctctagga tcttgactta cgtagccaat attttttgat atactttctt gtgttaagga
    17341 ttgtagcgag ttattttcaa ataaaatttc cccaaccaat tttccgtcat aaagtcttgg
    17401 aataagtcca ttcattaact ttaatagtgt ggatttccca ctcccagaag aaccagtaat
    17461 caatgtaaga ctgccttttt ttattgaaaa attaaggtca acaagcgctt gttccttaac
    17521 atccttatat gaaaaagaaa cattgttaaa ttcaaacaat tctttcacct caaatcaaaa
    17581 tacttaagaa aaaaatagta acggaaaaca ggatgatata gtctaataag ctcatgtttg
    17641 ttttatagta agaacttctt ttcacaggag attctatccc ctttgttatt gcattagcgg
    17701 tcatttcttc agcaatcata gttgcacgta taagaacggg aactaacaga aattccaaat
    17761 attgcagagg ctgaaaaaaa gacaagcgaa tgtttctcat ttttaaagat tctttaatca
    17821 ttcttatttc agaacgataa gtcggaaaga atctgatcac tactgtaata cttaacatta
    17881 tttttttcgg taaatgtatt ttttctaaag aggataataa ttcattgcta gtatagacac
    17941 taaccaacaa ccatgagatc attacgaccg gcaacattcg aaatacaata tataaggatg
    18001 tcgcaaaaaa tatagtaaaa aactctatct tgatagcatc aataattttc attactaaaa
    18061 acagaactga ataaatcaag atgaaataaa tacatgtctt tattttacga aacatcaata
    18121 ctaaaactgt agcaataatg aaacacccca cttgaaatat atttcccttt cctaaaaaaa
    18181 taatgaccgg aaaaaaaata tttaaagtaa tcaaggttaa cggattgatt tttgacactc
    18241 tttcacctcc aaaaaataag atcgcactgt tatcttaagc tgataaaaat gcaccgttat
    18301 tcagataaca gtgcgatctt atcaaaaagc ataactgaga acaattctca atgcaagctt
    18361 tttttaatta actcagtaat tcaatccacc caggaacaga aaattgatac aatcttctgg
    18421 ttaatagcat agtcttttct tttgaatagc tatgcttcaa tatatcatag aatgtagcgt
    18481 agaatgagta aatgtagaag tggatttctt cttcattaaa gactaaattt tgattgatca
    18541 ttccgatagt ctttaactta tctagacaac agactgtctt tttcgtcaca aatgatgtaa
    18601 ttttttcaac aatatcttga tatttggttc cagctgattg gaataaaatc aaatcgaata
    18661 tacgtttatt ctcaaaaata taattaataa atggatctaa ttcattgaag ttctctctcc
    18721 aacttttctc tatatctata ttttctaagt ttaagaggta tttggtaaac tcttcattat
    18781 acatattaat aagcgcatta atatgcggct gaataatttc ttcaaataac gcttccttag
    18841 aagtaaaatg tttataaaaa gcacccgtag ttacgcctgc ggtcaggcac agttgcctta
    18901 tcttagtttg ctgaaatcct tctttttcaa aatggcttag tccactttct attaactttt
    18961 ggtgagttat aatataatct ctggtttcta tgggtacttc ctcctaataa gatgaattaa
    19021 ctttattatc ttacacagga cttgaagttt caatcgttaa cgtttattac tgaactcatt
    19081 ccttagagct aaactaatga gaaagagcag aataactgat acaaaaagca atcctacaag
    19141 aattaataga ctcatctaaa tcattcacct tttctaaaat aatccgagaa agatttaaaa
    19201 aaattatatc cagctattag gataagtcct atcataatca ctccaatagc cattttctta
    19261 tcctccctaa aaataaagtc ttttacttaa aacctttttt gtaatcgtca ataaattttt
    19321 tccctttatc agttaattga aatcctatat ctaaatagtc ctcatatcta agtttagcca
    19381 gcatctcact tacagatgct ggactaacac ttagaacttt tgctaaatca ttatttttta
    19441 atttcttttt tctttttgat aattcctgaa ttgctagtaa ataaattagt atagtagtcg
    19501 ttttaatttg taccatacct accctccaaa ttatttcgaa aggccttcag caattttatc
    19561 caggttccat ttcatcatcg catagtaact atcaccatct tgtccttttt ctgcaattga
    19621 atcagtaaaa atcgttgaat agataggaat attggtatct tttgatactg ttttcattgg
    19681 tctatcatcc acactgcttt ctacgaataa ggagggaact tttgttgtgc gtaatttttc
    19741 aactaagtgt tttatttgat ctggtgttcc ttcttcttca gtgttgattt cccaaatgta
    19801 agcagaaggc acattatacg ctttcgagaa atatttaaag catccttcac ttgtaacaat
    19861 cattttttta tcattcggaa ttgaagcaaa tttagattta gcttctttgt ctagagaatc
    19921 caatttttca atatacttat ctagattttc tttatagaat tttttattat caggatcttt
    19981 ttccgctaac tgtttttcaa tatttttagc gtaaataata ccgttttcta aatttaacca
    20041 agcatgagga tcttccttcc ctttctcact ctgaccctct aagtaaataa catctatgcc
    20101 atcacttgct gcaaaatagt ctttgttttc ctctttgttc gcatttttaa ctaattttgt
    20161 aaaccaagca tttcctccag tttccaagtt aacaccgtta taaaaaatca aatctgcttt
    20221 tgaagttttt tgaacatctt caggcaaagg ttcatattcg tggggatctt tcccaatagg
    20281 tacgatactg tgtaaatcaa ttttatcttt tgctatattt tcggtaatat ctgctaaaat
    20341 cgaattagta actactactt ttaatttttc gttcgaagcc actttatctt tgtctttact
    20401 atttattgaa catgccccga atataaaaag agcaatcaaa atccccagac ttcccagtac
    20461 tgctttccat tttctcatca taacgatctc tccttctttc ttaaaaattt ttgttttggt
    20521 gagattagaa aactaattac aaataagaat gccgctgtta aaacaatact tgatcctgct
    20581 gctatgttaa aactataacc aataaacaat ccaagcaatg atgaacttgc tccgactgtt
    20641 gcagaaataa atatcatagt tttcaagcta tttgcatata aatatgctgt agctgctggt
    20701 gtaattaaca ttgcaacaat taatatagtt cccacacttt gcatagctgt tactgctact
    20761 aaagttaata gaaccatcaa tagataatga taaaaactga cattcatccc tattgctttt
    20821 gccattaatg gatcaaaaga agtgattaat aattctttaa aaaatagaac tactataacc
    20881 aacactaaaa ccgaaacgcc aattgtaatc cacttatcaa tatcttgtac agctaaaata
    20941 tttccaaata aaatatgaaa taaatccgtt gagctatttg caactcctat aaggataact
    21001 cctagtgcta aaaaagaact gaaagtaata ccaattgctg tatctccttt tattacacta
    21061 ttgcttttta taaaagtaat aatgatagaa gctaatagac caaatacaat cgcaccaata
    21121 aaaaaattaa tacctaaaat ataagaaatt gctactcctg gcaaaacagc atgagaaata
    21181 gcatcaccca taagagacat tcctcttaaa ataataaaac aacctactgt tccagaaaca
    21241 atccctataa ctacagatgt aattagagca ttctgtaaaa agtgaaagtc atacagacca
    21301 ttaataaaat tttgaagcat attattcccc ctctccaata aatatagtat ctccataggc
    21361 tttttttaaa ttttcttcat taaatacaga ttctacactt ccgtgagcaa ttagatttcg
    21421 attcaaaaga ataatattat caaaatactt ctttaccttg cttaaatcat gatgcacaat
    21481 tagcactgtt tttccctgat gtttcaattt ttttaacgta tccataatta ttcgttcact
    21541 aactaagtct atccctacaa atggttcatc taaaaatatg aaatccgcat tttgagcaag
    21601 acatcttgct aaaagtacac gttgaaattg tcctccagat aattctccta tttgatgatt
    21661 agaatatttt cccatatcga ccttttccaa agcctttgaa actctttgcc attctgcatt
    21721 tttaattctt tgaaaaactt tcattccggc atatgttccc atagatacac attcttttac
    21781 ttttattgga aaagtaaaat caatatcagc tttttgctct acatatgcaa tcttcaccag
    21841 ttttttattg atatcttcat tattaatgga gacctctccc tgatgaggga taatatttaa
    21901 cattgcttta agcaatgtgg attttccagc accatttgga ccaataatac ctgttaaagc
    21961 accttcttgt atttttaaag aaatctcgtt caatgctaaa aaatcgttat aacaaacatt
    22021 aagctttttc acttctaaca taactttctc cttctaaatg ataatgatta tcacgatcat
    22081 atggtataca aaaattatat tttagtcaac cctaaatttt atagtttaga tttttttctg
    22141 ttgctgaaga ttagaatgac aaaactattt gaaactacgc gtatagtaag tagctttcaa
    22201 aatatataaa aaaggagcgt tctcaaatga gaacatgctc ctaaaactac tttattggcg
    22261 tatggaaaaa ttatacttga agtggatagc gggttgaata agttgataag catcaaaaca
    22321 tttataaaaa ttattcaaga ctatgtctgt tccatataca aatatgaaaa tcagtaaact
    22381 ccatttaact tttttcgtag tgtgtctttt tatacatgtt tatcgttatt aatagtgaat
    22441 aggaaaaaaa ctatttttga tcattgacaa ttaaatacaa tttttgaata acgatccctg
    22501 ctattcgagc tagaaactca agtacgccat gacctgatta gcgacaaata cttagagatt
    22561 tctttttgtt ctgggaaaac aaaatgccat gacaatagca gcaaataatg atacttccgt
    22621 aaggctcgcc gtaaactggg gagaggtgaa attcctatgt gcttacccaa agcaattcaa
    22681 aaatttcata actactattt taggagatga ttttatagaa tccagagtct acctgaataa
    22741 aaaacaaacc tgtgcatttc taggaatcga taataatact ctagataagt ggattcgtat
    22801 atataatttc ccagtaataa gagttggtgg tgtagttcgc tttgacgtag aagatattaa
    22861 agactttatg aatacattta aaataacttg aaattttctt ctcgccgcat atactaaaat
    22921 tagtgaaata tactgcctta ctttttttaa ggagtgagga ttttgcctac aaagctttca
    22981 aatgggaaat ataaaactaa tctgagatat ccaaaaaggt ttaaagaaat aacaggaatt
    23041 gcatcagaaa agtatcaaaa aacatttcca aataggcaat tggcgattaa agccgaaaat
    23101 gatatgaaga agaaaataga aaaagttttg agagaggaga atgcaaactc attagaatta
    23161 aaaggaaaaa taacgtttaa aaaattttac gaatctaaat ggcttcctag atatgaatta
    23221 ggtcaaacaa ttagatcaaa caggcctcct agtgatataa ccattagtaa tacaaaagat
    23281 atatttcggt tgcatatttt acctatgttt ggtgaatacg caatgaatta tctaaatatc
    23341 aatactgaga taatatcaga tgaattaacc aaaaaatcga aagaatatgc aaatataaaa
    23401 atcataaaag gatatgttcg ttccatgttc gatattgcag aaatactaaa ttatatcgaa
    23461 tttaatcgaa ctactaaaat tattcaaagt atcactgcac ctaaaaaaaa tgctttagaa
    23521 gaaaaaagaa tacaagaagg taagcaagca ctatcatcaa aagaacttac taattggata
    23581 gaagcagtaa atgatgattt taataatcat ttacttactt ttcatgatta cacacttttt
    23641 atgattaccc tgtatcttgg tgatagaaaa agtgagactt acgctttgca atggaaatat
    23701 attgattttg aaaaacaaac ggtccgtctt aaacatactt tagataaata tcaaagaaaa
    23761 aagtttacaa aaggtcgaaa agataccgtc attcaagtac cagaagttgt aatgacattg
    23821 ctaagtgaat ggaaatcggt acaagctgat caattgttga aattaaaaat taagcaaacg
    23881 ttagatcagt atctctttac ttatactaaa ccatctggag aagtaaattg tcctgttcat
    23941 gccgattacc taaactatcg aatcaattca attaaaagaa ggcatcctga cttagttcat
    24001 ttatctccac acaaactccg tcatacttat gcaactatag ctagacaagg tggagcagat
    24061 atgaatcaaa tttcaaatgc tttaacacac tcagatattt ctacaacaaa gatatatgtg
    24121 aacacaccag atatagttga taaggctgtt tttgaagctt ttcagagagg attgaataag
    24181 tgtgattgat atgtgttctt aaatgtgttc ttaaaaacaa aagaagcact ttccaaataa
    24241 ttgaaaagtg ccagaaacgt tgatatataa aggatattaa cgttttgaga attgtgaagc
    24301 tttacgtgct ttcttaagac ctggtttttt acgttcgacc atacgtgcgt cacgagtaag
    24361 taagccagca cgtttaagag ctaaacggaa gtcaggatca acttctagca atgcacgagc
    24421 gataccatga cggatagctc ctgattgtcc agcgtatcct ccgccgttta cgttaacaaa
    24481 tacgtcatat gcgccttttg tttcagtaac agcgaaaggt tgattgatta cttcacgcaa
    24541 gtcagcatgt ggaatgtatt cttcaacatc ttttttattt acagtaattt tgccagttcc
    24601 tggtactaga cgtacgcgag caactgcgtt tttacgacgg cctgtgccga tatattgaac
    24661 ttgtgccaat gaaattccct cctattagat taagtttgtg atgtctaaaa cttctggttg
    24721 ttgtgctgca tgtggatgtt ctgctccacc gtatacgttc aattttgtaa attgtttgcg
    24781 gcctaaagtg tttttaggaa gcataccttt tacagaagtt tcgatcaaac gacgagaatt
    24841 tttagcacgc aattcgccag cagtgattga tttcaatcct cctgggtagt tgctgtgacg
    24901 gtaataaatt ttgtcagtcg cttttttacc tgttaatttc actttgtctg cattgatgac
    24961 gatgacgaaa tcgccagtat ccaaatgagg tgtgaaagtt ggtttatttt taccacgtag
    25021 tacagatgca acaactgttg aaagacgtcc caatggaaca tcagttgcgt ctactacata
    25081 ccatttacgt tctacttcgc ctggtttggc catatatgtt gtacgcacgt tattttcctc
    25141 caatttaagt tctgatgttt gacatacact tcgagtttcc ggggctcttt gtggggcaaa
    25201 caataccatt taacatatta cccgctaatc tagtcaatgt caacgttttt ctgcttgatt
    25261 gaaaaattta tatgaaactt tacttctttt ctttctttta tataaaaggg tcgtttcctg
    25321 tcaaaatgaa aaacaaaaag aggctgtgac aaaagtcttg tcacaacccc ttattttgaa
    25381 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatccata aaacataaga
    25441 agctgttttt taaaattgtt tcttgtgact cggagctgaa cggcaggctt acaacctctt
    25501 ggtaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag
    25561 gaactatttt gacgtttgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct
    25621 ttttcggtca agttcaacaa cagtttttac gcgtcgatct ctggcgcttt gcccaaatga
    25681 gcttggatca tccagatacg tttttctgtg gctgttttga attcgataaa gatatcttgt
    25741 gttggatcgt cgccttcttc tccagaaact tcgattcctt tttggtaaag atctgctaga
    25801 taacgatagc ctgcaacaag tgtttccaaa cgttctggaa tagtacgatc ccagcggcca
    25861 atttcatcag ggatttttgt gttatctgca aattcacgca atgtagaata aggtgaaccg
    25921 tctaatgtga tcaagcgttc ggatacttca tctaactgag cgttcaaatc atccatgaac
    25981 tcatccatca ttgggtgcaa tgtaaggaaa cctggtcctc tcatgtacca atgtgtttgg
    26041 tgaacaacaa cagacatttg gcttagatct gcaactaatt gatttaatac ttctttggtt
    26101 tgttggaatt tcatctttct tgcctcctaa cattttctta cacttaaaat aatagtagac
    26161 tttcaatgaa aagtacaaca aaatcattta cctgagaacg attttcagtt cgctttttta
    26221 tccgtaaagc caatcatttt tcagaatcag ctaattcttc tcgcagttga gcaaattgct
    26281 gataagtctg tgctaaatgc ttttgtgtcg aaacaaataa tggatagatt ttctgatatt
    26341 ttttatgatc agtttcttgt actccaactt gctctttcaa ttctatcaat tcctgtgctt
    26401 cactgatact ttggatcttt ccgatgcttc tccagcctaa aatcacagct cctaatgcgg
    26461 atgcttctgt gacagtaggg aaaacaagcg ggtaacccag tacatcagcc atcatctgac
    26521 gaaaaatcgg agattgagca aacccgcctg tagcacggat ctctgtgagc tgattttcct
    26581 ggcaaactcc ttttaaaatc cgataaaggt tgagacagat cccttctaga atcgccttca
    26641 tcatttcttt ttctgtatgt tgccgctgta aaccgataaa actgccaaac gcatctgcgt
    26701 cccaaagcgg tgctcgctca ccaagtaaat agggatgaaa aatcagacca ttggcacccg
    26761 ctggcacaaa agcgatagcc gaaagcagtg catcatatgg attttctcct ttttctttag
    26821 cagattgaac gacttcctgt aaaaatgtat gccctgccca atcaaagatg ccagcaccat
    26881 tgctagtcgc tcctcccact acccaatgtt tctgatccag aacataacaa aacgtctcca
    26941 tttctggatg gaactgaggc tgagtagtaa taaaacgaag tgctccgctc gttcctacgg
    27001 tcaaagcggc gatcgtatca ccaaaagcac ctaaccctag attagaaagc ggcccgtctg
    27061 cccctcccaa aacaaatgat atatcatttg gcaaacctag ttctgctgct gtttttgatg
    27121 gtagacctgt tacttgatag gtggaaggaa agacttgcgg caattgctct attcgaatgt
    27181 tcaagtagtc aagcacttct tctgcccatt ggaattcatg gatatcaaaa tatccagttc
    27241 ctgaagctgt tccgtagtcg ctgatcagtt cgccaaacaa tctataaaag atatagtctt
    27301 tgataccgac aaaataagca gtttttttga ataattcagg ttggctgttt tgcagccact
    27361 tgattttgtt caatggtgtc attggatgaa tcggcgtccc agtctgccga taaaattctt
    27421 tgccaagcac ggtatatttc agtttttctg cttcgtccgc tgctcggtta tctgcccaag
    27481 tgatcattcg agataacggt gttttctctt catccaacaa tatcaggcca tgcatcgcag
    27541 aggagaagga gacaaattga atgattgtgt ctgaaggcaa ctgtttaatt acttctttta
    27601 taacggtgca gacaccttga aagatcaatt cctgatcttg ttctgccatc ccttgtgttt
    27661 cttggatcaa agggtagcga aaataagcgg atgctgcttc ttgggcttca tctgtgaaag
    27721 caactgcttt ggtttgtgtg gttccgatat ctaatccgat tgaaacgttc attttttcac
    27781 ctcaaatttt atgtatgtac aaaaatttag tttaagcttg cgtatcttgg ttttgaaaag
    27841 ggggagatgg cgtgttactt tattttatca ttggttgctg tatcggatca tttttatgtt
    27901 taaccgccca gcggattccg ttaggtcact ctattattta tcctagatct cattgtgtca
    27961 aatgctgtcg ttcgctttct tggtatgaat tgattccgat tctctcgatc atcgtccaac
    28021 gatttcgctg ccgctattgc aggtgtcggt tgcccttcta ctatttgttg gcagaagcct
    28081 tatgtggtgg tctttttgcc tggttcttta ctttttcttc cgcaagaaat ttttcgacgt
    28141 tcatatggat gctttcggct ttgctatttt cgttgatgga tttattttac ttcatggtcc
    28201 attctcacac gctattctgt tcttgggcaa ttttatggat tttttggttg caggccggcg
    28261 tcttccaatg gcaaagtgtg ctgttaacgt ttatcgtagg cggtgtgtgc cttcgctatg
    28321 gtcaatccta tctcggagct ggcgatactc tgttgatgct ttcttggagt ggtggcttgt
    28381 ctctagaaga gttgcttcag atcttgtttc ttgctagtct gtttggaata tccgtttttt
    28441 ctgtttactt tatctgtcat aaaaaaaagt tggaaaagct gcctttcatt ccttttttga
    28501 gcagtgcgtt gctgattgtc cttcatctta ggtattgaaa aaaggttgtg acaaacgact
    28561 tgtcacaacc tctgatatat agtgttctct taccaaatcc ttgtgacttg gagctaggcg
    28621 acaggttcac aacctctcca ctaaatggtg ttcaatcagc tgaccctcag tattagctca
    28681 aaatgctggt tttacaacct ctgatatacg gtgttccata agtagctttt tcggcttctt
    28741 atttcttgac gccccaatgt cgacgaagga aacctgcacg tccagaacct tctcgataaa
    28801 gattgtaatg gagcttgttt ttcttgtaat aatcctgatg gtaatcttct gcaggataaa
    28861 attcgcttct tggttcgatt tttgtcacga ttggcttatc gaatcgcccg ctgtcttgca
    28921 atgcttgttt tgaacgctct gcaatatttc gttcttcttc atcattatag tagatcactg
    28981 gacgataaga atcaccgcga tcagcgaact gtcctaatgc gtcagtcgga tcggtttgct
    29041 gccaatagat ttccaccaac tgatcataag aaataacttc tggatcatat tcgatctcta
    29101 ctgcttctgt atgccctgtt gtgcctgttg ttacttgttc ataagttgga tttggtacat
    29161 gcccgccagt ataaccggat gtcacagaaa tgatccctgg ctgctcttca aatggtttga
    29221 ccatacacca aaagcatccg cctgcgaaaa ctgctttttt tgtcatataa aatccccctt
    29281 ttttctcatc ataccttgat ttccaaaagg aggataacgt tttgcttgtg acagaaaaaa
    29341 cggaaactat tatatacgtg atacggcttc tgataaatgc caaaacgaac gtacttgatc
    29401 aaaaacggtt aaatcaggaa tcacatattc agcagaagga agatttatgt tttcacctat
    29461 tcccaataca tgcatgttac cagccaaagc tgcttgacaa ccagcttctg aatcttcaat
    29521 gaccaagcat gcatttggcg gtacattcaa ttgctgtgct cctttgagaa aaacttctgg
    29581 atcaggtttt gcttttgata cttgcgtgcc gtcaataagg acatcaaaat aacttgtcaa
    29641 attcgttttt tctaaaatga ggcgtgcatt tttgcttgct gatcccaatc cgatcttgat
    29701 gtttgttttt ttcaagtact caagaacagc tattgcacca ggcaaaacgg atgtttcatc
    29761 cattttttga atcgcttgaa gatatagatt gtttttttgc tcagcaaagg ctgatttctc
    29821 tttttctgag aatttctgtt ccatttttcc aaatgacagc aatcgctcta gtgactcggt
    29881 tctggatatt cctttcagtt gctcgttttc tttctcagtg aatggaatgg acagctggtt
    29941 agctaaaacc agccacgctt cgtaatgata acgagctgtg tctacaagaa cgccatctaa
    30001 atcgaatagt gctgctttga ttttcatcga ttacctcctt gaaaatgaac gacttgaagt
    30061 aaagatgcga tgtttttagc tgtgcgatga agattatcag tggcttgctg taaagcttct
    30121 tgtaaaggca cagctgcagg aaggatcgga aaaacggccg aaatgccaac atctgctgct
    30181 ttttcgcttc ctttaccaac acttccgcaa atagctatca caggaacgcc ctctggtact
    30241 cttctagcaa tcccaactgg cgcttttcct tttagacttt gtccatctag acgtccttcg
    30301 cctacgatta cgaggtctgc ttgctgtacc cgtttatcga actccagtaa atccaatacg
    30361 tagtcgatgc cagagactat tttccctgaa gcaaatgcta acagtcctgc acccattcca
    30421 ccaccagcac tactgcccga tgcctcgatt acttgagggg caaatctcat ataaaaatca
    30481 tgcatggctt ggtcagccac tgctaattca ttttctttca agcctttttg tggtccgaaa
    30541 acaaaagaag cgccttcttt tccgcataaa agattagtaa catcagtaat gacagtgatt
    30601 ttggctttaa tcagctcaat aggcacgttc gttttatcaa ctgtagcaat tttacctaga
    30661 ttttctccca gtgcttctac tttttgatta tcttgatcgt aaaaagcata acctagacct
    30721 tctgccatgc cgatgccgcc atcgttggta ctacttccgc caacgccgat gatgatgtgt
    30781 cgaacacctt gttgcaacaa ttgtcgtatc agttctccta ctcctcgcgt ggataaccta
    30841 agtggctgtt gttggtttag tgggacatct ttcaatccga cgatttctgc catttcaaaa
    30901 atcgcagttt gaccgtctga agcgtactgg atttctttgt ttttgccaaa tgcattagta
    30961 acgactgctg tttgccgctt caaccttaaa ctgtctgcta atgatgaaac cgttccttct
    31021 cctccatcgc ctataggtag cagatcatat tcagcctgaa caaaaacttc ttgaaaacct
    31081 gattgaatgg cttgtgctgc ctctaaggct gtcagacttt ctttgaaaga atctggcgca
    31141 atcagaatac gcattatttt cccctccgtt ccaagatcag ttctttttcc agctggtatt
    31201 ggtctccatt gacaaaaata ggtaatgttt ctttcgtctc tttttttatt actatttgtt
    31261 tttttgtcaa aacaaatagc aacctgtgat ttttccaaat aaaaggaaaa gaaatttttt
    31321 cccatgctga aggaagtctt ggagaaatag aaagagttcc attcgttact gtaatacctg
    31381 aaaaaccaaa tataactgct agccatagtg ctcctaatga agcagcatga attccgtcat
    31441 ctgatgaatg cggatctggt cctaaatcaa tcaggcacgc ttcttggaat aaatgatacg
    31501 cccattccat ttcttggata cggcaagcct ctatggcgtg aatcgcttta ctcaacgatg
    31561 agtcgtggat tgttttgttt tcatagtaat gcaaattctt agaaacgatt ggttgagaaa
    31621 aatactcagg taataagtat aacagcatga caagatctgc ttgcttgctg acttggagtt
    31681 catttacttc gctacgggca taatcaagta aaatagactg gctgccagga cgttctttgt
    31741 aagtggtcaa atcaataact ggcttactga gatacgtgtc atcttgcgga atcagctgtt
    31801 ctttgttagg cactggcaaa tataacttat ctaaaaaatt ttgacattct ttcaaaaaaa
    31861 gcggatcgcc agcttgatat ttaacagcta aggccacatt ataatgcgcc agatagttcg
    31921 tatatgcatt attgtcgata tattctgtgt attcatctgg accaatgact ctgtgtatct
    31981 ctaaatgatc atcgactttt acagctctgc tcagccaaaa agctgctgtt tcatgaatga
    32041 gcgtttttcc ctttgtttct aaaaacgatt gatcctctgt tgcttggaca tactcttgaa
    32101 cggcataagc aaggtctgca ctgatatgat gttctgcggc agctgaagca acttcttgtc
    32161 ttgttccggt tttgatattc aatgcagcaa atttgggtgt ctcttcctga ccagtgtgtg
    32221 cgctttccca tgggaaaagt gcaccttgat agtgataatc tcttgctttt tgccgtgcgc
    32281 cttctaatcg atcataacgg tacagcagca aattctttgc tacttcagga aactgatata
    32341 aaaagaaagg caggataaaa atttccgtat cccagaaaac atgcccttta taaccttcgc
    32401 cagttagtcc cttcgcccct acactaacac ttggatcatt gcggggagtc ataatctgta
    32461 aatggtaagc agcgaagtcc atcgctaact gatcgaaaga agaatcgcct tggatcgtta
    32521 tccgatgctg atgccaaaaa tctgtccaat aagacatcga ctgctctaaa atttctgtat
    32581 aactaacgct tttctgggct tgggcaatac ttttcttaaa cgggtctgtt tgctgaagag
    32641 aggtagagag cacgatgatc ttctccagcg taaaggacgt attttttctg atcttttctg
    32701 aaaaggaagc ggtgatccgg cgattttttg cttggaatac acctgcttta ttgaatttag
    32761 ctgcaatccc aatatcaatt ttactttctg ttgtctggta ggatgcactg atcaaatcgt
    32821 caccaaatac tcgcagttct ttttcaacca actgctgtgt accaaaattg gtttgctgtc
    32881 catcgatacc cgttttcaca tctacttggc agtcttcggt caacggcttg atgaccactt
    32941 tcagggctgc ctgatggaga ttattttgat cagcaacacg acaagtaatg acttccactt
    33001 tttttccttg agcagtttca atgagcgcat agcgtttaag ctctcccatt tctaaatcta
    33061 gtgcccgctc gtatgcaaga actttcatcg tctcaagaga aaaatgttca ccgtcgattt
    33121 ttatttccca tctgatgaca tctgggagat tcactaactc ggctgattct gctcctaaag
    33181 gtcggttata tacaccagct gcaaacattc cccttacttg attccgatag tcttcctcct
    33241 ggcttcctct gataccaaaa aatccatttc caatggtcat ctgactcgca tacttattga
    33301 tatactctaa atcaaactgt ttttcttcta ctttcataag gtaataactt ctcctgtttc
    33361 tgccgaacga tataaatttt ccaccagatt ctggatgaca tatccttgcc agccatctgc
    33421 aatcatctga gtctggtcac cgagcgcccg attcacaaaa tgagccatac ttagttcatg
    33481 tgggtcatta actggcgtgg tcttttgtaa ggaaaccagc tttccatcta catcttcgaa
    33541 tagttcgcct ggatacagat tagcacctgc ctgatcgcca caaaaagcca cattcagttt
    33601 gtcttctctg atattcaaag caaacgaagt attcagttgg atgatcttgc cgttttctaa
    33661 ttccagtgtg ccaaaaattg catcttctac tgtgaatttg cttggatccc atttcccgaa
    33721 tttccctgaa ttttttttcg taccaatttt ttggaagctg tacgcccgaa cgctttttac
    33781 tttaggaaat ccagtaatat acatcgctgt atcaagcatg tgaatgccat aatcgatcaa
    33841 aggcccgcct ccttgaagtt ctttgtcgat aaacgttccc cagcctggaa caccagaccg
    33901 acgaagcgca ttcgcttcta cgtaataaat atccccgaga acatcaagat gattcttgat
    33961 cattgttgct tcgtcagaaa aacgatgctg gaatccataa gccaaaatct gcccgttttt
    34021 atcggctgtc tctgccatct ttcttgcttg atcagctgtc atagctggtg gtttctcgca
    34081 catcactgaa gcattagctt cgagggcagc caatacatgt tcgaagtgaa agcgattggg
    34141 tgtacagaca ctcacgatat cgggagaaat ctcattcagc atttgtgtaa ctgatgtata
    34201 cgcatgagaa aatcctgtac gtcggcaaaa gtcttctgct cgctggctga ctggatcaac
    34261 cacagcggcc aattctaaga catctgatct tttttgataa taagcagcat gtactttttc
    34321 tgctacttgt ccagcgccta tgattgctac tttcttcatt tatttctctc tcctttgctt
    34381 atttacttat tgttattctt tctctattct ttctctaaat catttaaatg acgcgctgtt
    34441 tccagacagt ttttcaagta ttgcaatgat tcctgataaa ttttcacttc attttctcct
    34501 cgcaaacgac attcatatac catataacct tcatactcat tttgaagtag cgtttgaaaa
    34561 tggcgagtaa aatcaattgc gccagatcct ggttggtagc gatgattatc tgctaaatgg
    34621 atatgaccca catactcttt ttgcgtagtc aatgcttttg aaatatcatc ttcttcaata
    34681 ttcatatgat aaaaatcagc gatgatctgc acatgtttca attgattaga gtcaatgtaa
    34741 tgacgcgcat ccgctaacaa attgatcata tggtcttggt atcggttcaa aggctccaaa
    34801 taaataaccg tacctgtttc ttcggctact ttatctaaat aacgaagtga ttcgctgact
    34861 gctttatagt ctccttcttt gcttcggggt gaaaccatcg gcggtaatcg ataagtgaac
    34921 attccccaag ctgctgggat aacgattcct ttgcctccaa cctcgcttag tgcacgcaaa
    34981 atctcagcaa tttgacgtac accattaaga cgccgttctt cgataaagtc accgatccac
    35041 ccatcgtagc cgttacaagc tgtcactact gggatccctg tttctttgat tgcctgtttg
    35101 actgtgtcga tattttctat taacaactta ccgtcgattt cgtattcttc aaatccacag
    35161 tctttgataa atctaaattt ttctaaaatg tctcttggga aaaatgcttg atcttgcgta
    35221 ccgaatttca ttgttctcct cctcggactt gaacgcccat tttgatactt tcttctgggt
    35281 gctggtctac ataacgcatg taagcttctg cgctttctgc aaatggcaca actggatcaa
    35341 tgatttcttc acaatccaag taaccgttca tcaataattc ccaacatgtg tcctcgattc
    35401 gtttacggtt ccatcttggg taatctgggt ttggttcact agatgctcga gcaaagacga
    35461 tttttgcatt attgaaatgt gcttcgcgac caagattaaa cccagctgga aaaggtttag
    35521 caaaggcaac ataagaaatg atcccgccat agccgatccc tattaaagaa gcttgtaatg
    35581 cggcagcact gccactcgtt tcaataactg aatctactcc acgtttatct gtcaattcct
    35641 ttagacgaaa accgacatct gctttcatag gatctagcgt gtaatcggca ccgtgttttt
    35701 ctgctaattc tctacgctgc gctatgggat cgatcccaac gatggtagta gcacctgctt
    35761 ttttagctaa ttgaatcgca atttgcccga tagcgcctaa accgaagatt gctacagcat
    35821 cacctgctcg aacatgtgct tcccggatac cacctaaagc gaattgagca ggatcgtaac
    35881 atactgcatt tttccattga tccaaccgat caagtttacg tagtttataa ttatcaacac
    35941 cgtttgtaat cagcgtttca cgaatgggtc cataagtgca gactaactct cctttttgat
    36001 actctgtcac caaactgccc gtttcttcga tcgtaccgac aatcatattt cctaattgaa
    36061 attcgccaaa taccacaccg cgttggcttc cttcttctct tggcaagaat aggcgccatt
    36121 cttctgaaaa ttcttcatcg ataaatggac ttaatcctct aaagtcgaca atttctgttc
    36181 ctggttttgg agtagcgaaa attactcgga tacgtacttc gttcttttcg atctcacggt
    36241 cttcgtaatc tttgaatccg gctattcttg gttctaatgc aactaatttt ttcatctttt
    36301 ctttcctctt tcttttcttt ttatcctttt acgccgcctt cgactaaatc acctttaata
    36361 aatcgttcag ataacgcata cataatgatg actggtaaag cggtcaccaa tgaagcggcc
    36421 atcattcgtc cccaaatgta gtctggtgta ctgaataatg tgttcaaacc gattggtaaa
    36481 gtaaattttt cggaggtcga caggaaaatc gaagcaaata aataatcatt ccacgaaacc
    36541 ataaagcaat aaacgaagac agaaatgatt ccagaaatag ccaatggtat aatgatccga
    36601 ataatgatct ggatccggct aagaccatcg atcattgctg cttcttcaat atcagtcgga
    36661 atcgtgtcaa aataactttt tagcatgaat atcgcagtag gaagtgtttg aacgaccatc
    36721 gtgataatca gtgctgtatt ggtatcatac aaacctagac cggaaatgat tttaaataat
    36781 ggaacaacga tcaaaatacc ggaaaacata taaacggtgt aaaagctagc attgattttc
    36841 gagcgagctt tgaagcgtag ttttgacaaa gcgtatgccc cgctgatccc aatcactact
    36901 gcagttacgg aggctatcaa ggagatcact aaactatttt tgaaatacat gatgaatgga
    36961 aaaatctcag cattgaaaat atcctgataa tgttcaaaag tccattcttt cggaaaaata
    37021 gtttgtgtcg ttgcaattgc ttcttgtgaa tccttgaatg atgtcatcag catgatcaaa
    37081 aatggaaaca aattgacagc taatagcaac aaaatcgtcg cgtaaaataa gattttcctg
    37141 ctctttttat ttttcatttc aagtccacct tctttcgtga gagcaagacg accgctgcaa
    37201 taatgatgaa caatagaatc gatatagctg ctgcttttcc aagatcattg aacgcaaagg
    37261 ctgtctgata caaataaacg cctaagatat tgactttatt cgtcagtaga tagacatctg
    37321 taaacatata gaacatccag atagatcgta aaacaatcac agttgctagt actggtttga
    37381 ttgccggcag tgtcacgatc ttgaacttct gccaagcatt tgcaccgtcc atttcagctg
    37441 cttcataaag cgttttgtcg actgtttgca aaatagccaa aaatgaaata aacgcatacg
    37501 gatagtatct ccagatagca aacaaaacga ccataaagaa gctagcaact ggctggtcga
    37561 accacagcgg tgcttcgttg aaaagctgca acctatctac taacagatca ttgacgattc
    37621 cataactatt attgaacata tatttccaag caaaaatcaa agaaatagat ggcgtaacat
    37681 aacttaataa aatcagtgaa cgcaccgttt tcctaaatct gaacttgcgg ttaagcaaaa
    37741 tagctacggc taatcctaaa gctgtactgc ctgctacaac taagaatgtg taagcaactg
    37801 ttaaacctaa agaacgataa aattcactat cacttaaaat ttcaagataa ttgcttaatc
    37861 cgataaaaac ggcgtctgag ttaggattca gcggcatatc gaaaaaactg atctgaatat
    37921 tggaaagcat tggaatcagc actaaaacag ctaataaaag catgcttggt gctaaaagtc
    37981 ccatggctaa ttttaagtca gaatgtttcc ttggacgatt cattgaaacc tccccctgat
    38041 aatctgccaa tagctatcta cttttttcga acaccattgg cagaacttct tgttatttcg
    38101 tatttttttg tgcgtcagct aaagcttgat cgacatcttc gtttccgaca gttacttggt
    38161 tcactgcttt tccgatcaca cctgagcttg tgatatctcc cattttcgtg aaattctttt
    38221 ccccgactag tccaaagact tgtacttcat caaaagcact agcaatctca gatggcagct
    38281 cgccaaaaga cttgatgact tcattttctt tgtaaccatc cagttcgact acttgtttat
    38341 tcactggttg agcaccacca ggagacatca agacccactt ttccatgttt ttcggttgag
    38401 ataagaaagc tgtgaatgct tcagcagcct ctttttgttt ctcgtctaga ccagaagaaa
    38461 tcgtcaatcc agatactgtg ccatagacag cttgatcttt ttgtgttggg atagcaaaac
    38521 cgatattttc tgagttcccc gcttcataaa cagaaggcaa aatgtaagtt gaatagattg
    38581 ccattggtgc tgttccattc atgaaagcat ctttgatttc tgtcacatca ttagagcctg
    38641 gcatcgtgta ttgggataag ttttgataaa acgacaatgc ttctctcatc ttttccgtat
    38701 cgatcgttac ctcgccttca tcatccaaaa catttgctcc atttgacaaa gcaaattgag
    38761 agaacgcttg ctcactcatt gttccttcag cagtaggcat agcaattcca tattgtttat
    38821 tctctttatc tgtgaaatgt tgagcaattt ttaaaatatc atcccaattt tctggttctg
    38881 aaaaaccagc ttctgataat ttttctttgt cataccaaat accttgaacc cagccgctaa
    38941 ttggtgcagc aatatagctt tttccatctt ctgaacgaac aaggtttttg gctccgtcat
    39001 aataattatc ttctccagct tcctcgataa cgttttggac tgccgtctga tcaatcagtt
    39061 catctttatc catcacttta gcaaaatcct gactgacttc cattacttct ggtaatttac
    39121 cggaacgagc taacgtaacg atttttgtat tgaacgctga ttcttctacc ggcacttgct
    39181 tgattttgat gtctggattt tctttttcaa aatcagcaac cagttcattg atcacactta
    39241 atcgttcctg ttcgacagaa gagtgcatga attgaatcgt tgtctgttgt gattcgttag
    39301 agctttgact ttcaccacaa gcagtaagaa gaaatccagt agctagcaga caggtagtaa
    39361 caataatttt tttcattttt ttccacctct tattttatta ttttcagcca catatattca
    39421 tacggctgga atatcaactt tcgttcttga ctgcaatgga taatttgtcc agaaaacagg
    39481 tctaggtaat ctccttttgg taaaagataa cctgctgatt gattggaaaa attatggata
    39541 actaagactt tctcttcctt cgcttcgcga ataaaactga taaaagaatc tgctgagtcc
    39601 agtaagtaaa tcaaactatt cggatggaag gctttttctt tttttcgaat agatagcatc
    39661 tgggtcaatt caccatatac tgctgttctt aatggatctg tctgcaactc tttctcaata
    39721 tctgctaaca aaaatttttg ccgattgatt tttcgattct ctcctgtttt ttctacgcct
    39781 ttgtaatcat ttcgacttcc cagcacactt tgaatataaa ttgccggcac gcctggtaac
    39841 gttaccaaaa atgaatgagc agctaaaaag cgtttgcttc tccattccgt cgtatctgtt
    39901 ggagaactta acgcatctag atacgtagcg tttatctcat atggactggt cgttccatct
    39961 gggtttttct tgtagtttac tcgggcaccc tctttcttca gctgttccac taattcaagg
    40021 atcgtttttt ccggcagaat acctcgtacc ggattcaatc cgataccgtc atgggaggct
    40081 aaaaaattaa aaaaagttgt ttttttcgat gcgaaatcga tggtttttaa ccattctttc
    40141 aacttacttg tattacccga acgaatcgct tgaagggtca aaggtggtag cgaaaactga
    40201 tagaccatct gtgcttcatt ctcgccattc ccaaaataac tgatattatc tttgtgtggc
    40261 acatttgtct cagtgatcaa taccgttcct ggagctgctt catcagcgat tgcacggaat
    40321 aattggatga ttttatgcgt ttctggcagg tgtatacaag aagttcctag ttctttccat
    40381 aaaaatccca cagcgtccaa gcgaataaat gaagcgcctt tttctaagta aaacaaaaga
    40441 atatcgatca ttttcaataa aacttctgga tttttaaaat tcaagtctac ttgatcttcg
    40501 ctaaaagtcg tccatacgtg tttttctttc ccgtttttgt caatgaacgt tgtaagtaac
    40561 gggctggtcc ttggtctagt cacgctgctt gtatctgtat ccagtggcaa atcaataaaa
    40621 aaatctttgt acgtatcatt caagtttaaa tattcctgaa accaagtact ttcggcagac
    40681 atatgattgc agacaaagtc gaacattaac cgtgtttcgc ttctcatttc ttctatatct
    40741 tcccatgtgc ccaaacggtc gttgattgcc tgataattta caacggagaa accatcatca
    40801 gatgtatatg ggaaaaatgg caagaaatgt acaactctaa atgtatctgc caaatacttt
    40861 tgataaaatt tcttaaatgt acgcaaagtt ggttgctgct cttcaaaaaa ttgatcccca
    40921 taagtaatca agaaaatatc ttcttcgtcc caatgcagtt tgcctgcctg gcctttcttc
    40981 tgctgataaa cactgacttt ttgttcaatc atagaggcgg ttttctcaga caaatctgag
    41041 tcgcgataaa tcgcttgtaa aagttttgta tacatcggat tcccctttct tttttgggag
    41101 cgttcccaac gatttgatca tattatggaa aacgcttact gtcaatagca tttttttaaa
    41161 taattttgat atacacaaaa atacaaataa gaaagcttat gtatcaataa ttaatagctt
    41221 cttaaaaaaa tatggtctct catgatcatg caagctattc ctaaattttc aaaatcatag
    41281 acaaaaaaga atgtctattg tactatggaa ttattctaaa acgctcccaa aaaggaggtg
    41341 catataaaat tgaaacctac tatttatgac attgcgaaaa aagctaatgt ctcaaaatca
    41401 actgtctcca gagtattaaa taaccagcca aatatctctg ataaagcaaa agcaagtgta
    41461 cttcaagcaa tcaaagaatt gaattatgaa ccgagcaaaa ttgcaagagg actgtcagga
    41521 aatggttttg acgcgattct agcattatct catcgtactt ctcatacgac ctatggcaat
    41581 cctttttttt cagatgttat tcaatccatt tctgccgttt ctgaacaaaa taacttcgac
    41641 ttgattctac aaacggcaaa aacaccagaa gaagagctag aaaaatgcgc gaaaaagatc
    41701 caagaaaaaa tgatccgagg aattattatt ttaagctcta cgatggatga aacacttctt
    41761 tttgaattgg atcaatataa tattcctatt gtggtaatcg gaaaagtcgg aaacgactac
    41821 acgaacatct actcagtgga cacggataat ttccaggata gttacgattt ggtggaaaca
    41881 ctgatcaaga atggacaccg tcatatcggc tgtctccatg ctccgacaga agtttatgta
    41941 tccaatgatc gtctcaatgg ttaccgtcaa tgcttatttg atcaccacct agatatccgt
    42001 aatgaatgga ttatagacgg cggctttcgc ttagaagata gcatggcagc aatgaatgat
    42061 ttaatgaagc tacgagaaag accaactgcg attttttcta ccgatgcatt aaaatcattg
    42121 agtatttaca aagtggcaga acaatctgga aagcagatac ctgatgatct ttcgatcgca
    42181 ggattcagtg atcccttatt gtcctcccta ttttcgccac agttgactcg gatagacatt
    42241 ccgacaaatc agttaggaat caaagccaca gaacttttat tccaactaat ccaccaccaa
    42301 taccctgaag aaaaaagaat cattctttca accgagatta ctctcacaga ttcgatccaa
    42361 caacaagaaa tgtagaacag aattataaaa gaacagaaac actgaggata agtcagttac
    42421 tctgttcttt tttcaatcac tcatccctcg ttaccccctc ccggtatttt agcgaatttt
    42481 tcttattttc catctgtttt agatagctgt ggataactat aatgtttgtg ttaaactatt
    42541 ttttccacag ggaagaaaaa actattttta accacgttgt gtataagttt ttatagttcg
    42601 tttttttaaa cacatttacc cttatttcaa tccatgttat ttggggatac ttatctctaa
    42661 aacataaata gaataacaga catgtctact ggttttgttt tattcgagga attattcaat
    42721 aatagctagc aaaatattaa aaataaccgc tctatcttcc catcaattta ttttgcattc
    42781 aaaaaatgat ctatctttac ttttgttgat tgacttctct tggaagatga tcgttccgat
    42841 accttaattg gatacgaacc gatgttgtta agatcctttc ccacttttct tctagttaga
    42901 caaattattt tagacaaacc aaaaaagagg ttgtgacaat aatttgtcac aacctctttt
    42961 ttaaaatatc tattttgttt gattgttcat tgcgtcttct gcagccattt gcgcttcaat
    43021 atctgaaata ctgtagacat tttcgcttgc tacaccgact tcttttggtt ccatgtcacg
    43081 ataacgttgc ataccagtac cagctgggat gatcttacct ataataacat tttctttcaa
    43141 accaagaaga ggatctttct taccgcggat cgcagcatcc gtcaagacac gagttgtttc
    43201 ttggaatgat gcagcagata agaaactatt tgtttctaat gatgcttttg tgatacctag
    43261 taagacaggt cgagaagttg caggaacgcc tcctgagact agcgtattgt agttttgatc
    43321 tttaaagtct gcgatgtcta ataatgtacc tggtaagata tctgtatcac ctggatccat
    43381 tacacgaact ttacgcagca tttgacgaac cattacttcg atatgcttgt cgccgatttc
    43441 taccccttgc atacggtaaa cacgttgtac ttcacgaagc aagtagtttt caacagataa
    43501 tacatcgcga acttgcaaca attgttttgg atcgattgat ccttctgtca atggtgcacc
    43561 acggtggatc atatcgcctt ctgctacttt catacgtgct gtataaggaa cagtatacgt
    43621 acgtgtatct gtttttcctt tgattgttac ttctttggta cgtgttgcag gatcttctga
    43681 gatatcgatg acatcccctg ttacttctgt gatcactgct tgccctttcg gattacgtgc
    43741 ttcaaagatt tcttggacac gaggaagacc ttgagtgata tcgtctccgg caaccccacc
    43801 tgtatggaat gtacgcatcg tcaactgagt accaggttca ccgattgatt gagcggcgat
    43861 cgtaccaact gcttctccga cttcaacgtc agagccagtt gcaaggttgc gtccgtaaca
    43921 atgtttgcag acaccatgtt ttgtattaca tgtgaagaca gaacggattg tcactgtttc
    43981 gacacctgca tcaacgattt gttttgcgat gtcttcggaa atcaattggt ttcctgcaat
    44041 gatcaattca tttgtttcag gatgacgtac ttctttacgg gtgtaacgtc ccactaaacg
    44101 atcttctaat gattcgatga tttcatttcc ctcgcggatg gcttggattt caagacctcg
    44161 gtcagtaccg caatcttctt cacggatgat tacatcttgg gccacgtcaa ccagacgacg
    44221 agtcaagtaa cctgaatcgg cagtcttcaa ggctgtatcg gtcatccctt tacgagcccc
    44281 gtgagttgag ataaacattt ccaagacgga tagtccttca cggaagttcg aaatgattgg
    44341 caattccatg attcgaccat taggagcggc catcaaacca cgcattccgg caagctgtgt
    44401 aaagttggaa atgttaccac gagctccaga atcagacatc atgaagattg gatttttcgc
    44461 ttccaaacct tccatcagtt tttgttggat gctgtctttt gcagcattcc aaacagcaat
    44521 gacacgttca taacgttcgt catctgtgat cagaccacga cggaattgtt ttgtaatagt
    44581 ttcaacttgt ttatgggcat tttcaatgat ttcttgtttt tcattcagta cactgatgtc
    44641 agcgatccct actgtgatac cggcatgtgt cgaatgtttg taccctaagt ctttcatacg
    44701 gtcaagcatc ttagaagttt ctgttacttt gaatcgtttg aagacttctg cgatgatgtt
    44761 tcctaagttt ttcttcttga acggtaatac taattcttgc tctttgatat gtgcaggaat
    44821 atcagtacca gcttcaacaa agtatttgtc cggtgtttgg acggtcaagt tgaagtctgt
    44881 tggttcattc agataaggga attcaggagg catgatctcg ttgaagatga tcttaccaac
    44941 tgttgtgatc atcgtacgtt ctttttgcca ttcagtaaat ggtttttcac ctaatgagtt
    45001 agggttcaca ccgatacgtg aatgtaaatg aacatatccg ttgcgccatg cgataacggc
    45061 ttcgttttga tctctgaaga ccattccttc gccttcgcgt ccttcttctt ccattgtcaa
    45121 gtagtagtta cctagaacca tatcttgaga aggagtaaca actggtttac catctttagg
    45181 gttcaagatg ttttgagcag ctaacatcaa catacgtgct tctgcttgtg cttcttcatt
    45241 caatggaacg tggacagcca tttggtctcc atcgaaatcg gcattataag cttcacatac
    45301 aagtggatgc aaacggattg cgcgtccttg tactaaaact ggttcgaatg cttggatacc
    45361 aagtcggtga agcgtaggtg cccggttcag caatactggg tgttccttga ttacgtcttc
    45421 taaaacatcc catacttcgt cttcttgacg ttcgatttta cgtttcgcat ttttgatgtt
    45481 tgaagctagt tcgcgttgaa caagctcacg catcacgaat ggtttgaata attcaatggc
    45541 catttctttt ggaagtccac attggtacat cttcaagaaa ggtccaacaa cgataacgga
    45601 acgacctgag tagtcgacac gtttacctag caagttttga cggaaacgac cttgtttccc
    45661 tttcaacata tgagaaagag atttcaatgg gcggttacct ggtccagtaa ctggacgtcc
    45721 gcgacgaccg ttatcgatca atgcatctac tgcttcttgc agcatacgtt tttcattttg
    45781 aacgatgatt ccaggtgcat tcaagtctaa caaacgtttc aaacggttat tacggttgat
    45841 tacacgacgg taaagatcat tcaagtcact tgttgcaaaa cgtccacctt ctaattgaac
    45901 cattgggcgt aaatctggtg gaataacagg gataacgtcc ataaccatcc agcttggttg
    45961 atttcctgaa gcgcggaatg cttctaaaat atccaaacgg cggattgcac gtgtacgttt
    46021 ttgtccttgt gcagatttca attcttcttt caattcggct gcttctgcat ctagatcaac
    46081 attatcaagt aattgtttaa tcgcttctgc acccatagca gcttggaatt cttgtccata
    46141 ttgttcacgt ttttcgcgat attcgcgttc tgtcaacaat tgttttttct ctaaattcgt
    46201 atttcctggt tcgatgacta catatgaagc aaaataaatg atttcttcca aagcgcgtgg
    46261 gctcatgtct aatacaagac ccatacgtga aggaatccct ttaaagtacc agatatgtga
    46321 aacaggagct gctaattcaa tatgtcccat gcgttcacgg cgaactttcg aacgagtaac
    46381 ttccactccg catcgatcac aaacgattcc tttataacgg atacgtttgt attttccgca
    46441 ggcacattcc cagtcttttg taggaccaaa aatccgctca cagaacaacc cttcacgttc
    46501 aggttttaag gtacgatagt taatcgtctc aggttttttt acttcaccgt aagaccagct
    46561 tctgattttt tcgggagaag ccaaacctat ttgcatactt tcgaatttat ttacatcgat
    46621 caaaaggggt tccctccatt tcatttaatg gcaaaccgca agacggctga tcttcgaccg
    46681 ccttgcagct catagaccgg attagtctaa attatcttct gcggtttcaa agttttggat
    46741 gacatcgtct ttttcatctt ccacttgttc agcagctttc ttttctaatt ctttggcagt
    46801 ttgctgttcg gcaaattttg tcaaagcatc aacagtgatc aaatcgtcgt cttcatcgtc
    46861 catgtcgcga agttcgatct ctgtttcttc gatatccaag acacgcatgt ccagacctag
    46921 tgattgcaat tctttcacca atacgcggaa tgattccggt acacctggtt ttggaattgg
    46981 ttcacccttg acgattgctt cgtatgtttt cacacgtccg actacgtcat ctgatttgta
    47041 tgtcaagatt tcttgtaaag tataagccgc accgtaagct tccagtgccc atacttccat
    47101 ttcaccaaaa cgttgtccac caaattgagc tttacctccc aatggttgtt gtgtaaccaa
    47161 tgagtaaggt ccgattgaac gtgcatgcaa tttgtcatcg accatgtgag caagtttgat
    47221 catgtacatc acaccgacag acacacgtcc atcaaatggt tcacctgtac gtccgtcgta
    47281 cagaacagtt ttcgcatcgc tggccatacc ggcttcacga actgtttccc atacgtcttc
    47341 atcacttgca ccatcaaata ctggcgttgc tacgtggata cctaactgac gtgcagccat
    47401 acccaagtgt aattccaata cttgcccgat gttcatacgt gaaggcaccc ctaaaggatt
    47461 cagcatgata tcgattggtg ttccatcagg taggaatggc atatcttctt ccggcataat
    47521 acgggataca acccctttat ttccgtgacg accggccatt ttatcccctt catggatttt
    47581 acgtttttga acgatataaa cacgaactaa catgtttacg cctggtgaca attcatctcc
    47641 agcttcacga gtaaagattt tcacatcgtg aacgataccg ccgccgccat gtggtacacg
    47701 taatgatgta tcgcggactt cacgagcttt ttcgccaaag atcgcatgca atagacgttc
    47761 ttctgcagat aattctgtta ccccttttgg cgtgactttc cctacaagca aatcgccatc
    47821 ttggacttct gcaccaatac ggatgatacc catttcgtct aaatctttta atgcgtcttc
    47881 cccgacatta gggatttcgc gtgtgatttc ttcaggtcct aattttgtat cgcgggcttc
    47941 tgattcatat tcttcaatat ggatagaagt gtatacgtca tcttttacca aacgacggct
    48001 catgatgatc gcatcttcat agttgtaccc ttcccaagtc atgaaaccaa ctaaaacgtt
    48061 ttggcctaga gccatttcgc cttgttccat agatggtccg tctgccagag tatccccttt
    48121 ttcgactttc tcacccaaat gaacgattgg acgttggttg tagcttgttc cggagtttga
    48181 acgacggaat tttgttacat cgtatttatc taatgcaccg ttgtcgcggc gtacgcggat
    48241 ttcagaagca tcaacgtatt caacgacacc gtcatgttta cataacaaag cagctcctga
    48301 gtcatgcgct gatttgtatt ccataccagt accaacccat ggtgcttgag gattgatcaa
    48361 cggcactgct tgacgctgca tgttggcacc catcaaggca cggttggagt catcgttttc
    48421 caagaaagga atacatgctg tcgcaacggc aactacttgt ttaggtgata cgtccatgta
    48481 gtctacttta tcaatagata cttctaagtt ttcactttga gcacgagcca taacaacgtc
    48541 ttgagcaaat gttccatctt cattcaatgg gctgtttgct tgggctacga tgtaatggtc
    48601 ttcgatatca gctgtcaagt agtcgatttg gtcagtaaca cgacctgttt cacggtctac
    48661 acgacgataa ggtgtttcga taaatccgaa tttatttact ttcgcataac tggacaaact
    48721 attgatcaac ccgatgtttg ggccttcagg agtttcgatc ggacacatac ggccatagtg
    48781 ggagtagtga acgtcacgaa cttcataacc ggcgcggtca cgagtcaatc cgccaggtcc
    48841 taaggctgaa agacgacgtt tgtgtgtcaa ttcgccaaga gggtttgttt gatccatgaa
    48901 ctgtgacaac tgagaagaac caaagaattc tttgatactt gccacaactg gacggatatt
    48961 gatcaattgt tgaggtgtca atgtttcagt atcttggatc gacattcttt cacgaaccac
    49021 acgttccata cgtgctagcc cgatacggaa ttggttttgt aaaagttcac cgactgaacg
    49081 gatacgacga tttcctaagt ggtcgatatc atcgacattg ccgatacctt ccatcaagtt
    49141 caagaaatag ctcattgaag caatgatgtc ggctggacga accgttttta cagctgcttc
    49201 tggatagccg tttccgatca cgtttacttc acgttcaggg tctcttggtg aaaatacttt
    49261 gatgacttgg acagtcattg gatctgtgac cacaccgtct tctgatggat agtaagtcac
    49321 tgaatttagg ccattatcaa tgaaaggagc tagtgtttcc atgacttggt gagtcagaac
    49381 tgttcctttt tcaacgatga tctcgcctgt ttctggatca actaatgttt cagctaatgt
    49441 cagattcaat agacgtgttt tcaaatctaa ttttttgttt actttgtaac gaccaacgtt
    49501 tgctaagtcg taacgttttg gatcgaagaa acgagcattc agcaagttac gtgagctgtc
    49561 tgctgttttt ggttcgcctg gacgcaaacg ttcgtaaacg tctttcaaac cttcttctgt
    49621 tcttgagtcg cttgcgtttt tatgcaagtc tttttcgact gtattgcgta atgtttcgct
    49681 gtcgccgaag atttcaaaga tcgtatcatc ggaaccaaat cctaacgcac gaactaatac
    49741 ggttaaagga atcttacgtg tacggtcgat acgtacataa gagatgtctt ttgcatcagt
    49801 ttccatttct agccaagctc cacggttagg gataacagta gaaccgaatc cttcttttcc
    49861 gtttttgtcg acttttccat ggaaatagac acctggtgaa cggactaact gagaaacgat
    49921 tactcgctct gccccgttga tgatgaatgt tccttgttct gtcattaatg ggaaatcacc
    49981 gaagaatact tcttgtgctt tgatttcgcc agtctcgcgg tttgtcaaac gcaatgttac
    50041 atgtaatggt gcagagtagt ttgcatcatg cgcacgagct tctgctactg tatactttgg
    50101 ttctttcaat tcataatcta caaattccaa agatagattg ccattgaagt catcgattgg
    50161 caaaatgtct tcaaacattt ctctaagtcc ctcatctaag aaccattggt aagagtctgt
    50221 ttgaatttcg atcaaatttg gtaattccaa cacttcactg attcttgcga aacttctacg
    50281 ttcgcgatgt tttccgtatt ttactacgtg tccagccaag ctcttcaccc ctctaaaatg
    50341 tcattcaaaa agactacccg atcaattgtt acaataatat ttcaaatatt atttttaggt
    50401 aacaacagac attttttgcg gcttttaggc aaaaaaaaac caaaaataga acattctctt
    50461 gaaaccttac ttcgtgagct tctacttttg tttccttata ttaaagccga aacggacaat
    50521 atttcgtttc tgccttttct tcatcagata gtttttgcaa cttcatatac tacacttatt
    50581 ttctataaaa gtcaacactt cctggcgttt cttctgcttt ttcgcaacat tttcttaatg
    50641 ttttcaattt tatgttttca attatctgac aattcttaat tgacagacct ttttatctcg
    50701 gttatacttt agaaaattca ttttgtggag gaacttctat gcgaaccaac atcaaccaaa
    50761 tcgcaccaac acaactgaat aacttttact ttagctactt tagataggtt tgtattcatc
    50821 atgatggata caaacgttaa acgacagttt gtatctatga tggctaaaga actttgtatt
    50881 gcagcaaaaa gccacgtaga caaaatgcta agtggctgag cagtgaaaat acaaagtaaa
    50941 agtatcaagc attctacttt ttcaatttat tttccgactt ttcaatccat ttggacattt
    51001 tgtctgaatg gattttttgt ttcccaaaat taaggaggaa aaagaaatga aaaaaaatat
    51061 aatcatcgga ttaggcgctg ccctgctact agctttagga ggttgcgcac aaaaagacaa
    51121 ttccgcagag gcatcagatg gacaaaaaac aattggcgta ttgcaacttg tacaacataa
    51181 ctcgttagat gctgcctata aaggattcaa agaaggatta gcagaagaag ggtataaaga
    51241 aggcgataat ctaacaatcg attatcaaaa tgcccaaaac aatcaagaca acctgaaaag
    51301 tatgagtgag aaactcgtca aagaagatcc tgatttgcta cttggaatcg ccactccagc
    51361 agcacaatct ttattaaatg aaacaaccac tatcccgatg actgtcacag cggtcacaga
    51421 cttggaacaa gcccatttag tcacatccaa tgaaaaacct ggcggcaatg tgactggcac
    51481 gacagatatg gtgccaatcg aaaaacaaat cggcttactg ttaaatatcg tgccagacgc
    51541 caaaacaatc ggcatcatgt ataacaacgg tgaagcaaac tctaagatcc aagctgattt
    51601 ggcagaaaaa gaattgaaaa aagcgggcgt caaagtcaaa gtactgacag caaattccac
    51661 aaatgatgtc caacaagtga cgaaaagtct agctaaagat gtagatggaa tctatattcc
    51721 tactgataac acgttcgcaa gtgcggcaag tgttgtcggt gaagtcgcaa aagaaacaaa
    51781 aacacctgtc gttgctggcg cagttgatca agtcaaagac ggcggtctag caacagtagg
    51841 aatcgactac gaaagcttgg gcaaacaaac agggatcatg gcagcaaaaa tcttagacgg
    51901 caagaatcca agtgaacttc ctgtcgaaac agcgaatgat ttggaactgt atgtcaataa
    51961 agacatggcc aaagcattag gcatcaatcc agacagcatc aaagaagctg aataaaacac
    52021 aggaggattc tcatgacaaa tttgctctct cttttcttat catctgcatc gcaaggggtc
    52081 ttatgggccc tacttgcgat tggtgtatac gtcactttcc gtatcttgga cattgccgac
    52141 ttgactgctg aaggaagctt tcctttaggt gcagggatcg cggcagtaag tattacaaat
    52201 ggctatcatc cattgacagc ttgtcttcta ggctttttag ggggtgcggc agcagggctt
    52261 gtttccggac tgcttcacac aaaattgaaa attcccgccc ttcttgcagg gatcatcacc
    52321 atgactggtt tatattctgt cacatcccgt gtgatgggtg cgccgaacat ctctttattg
    52381 ggtcagccaa cgatctttca ttgggtcgag tcattcggca taacaaaaga caacgctgta
    52441 ctgatcgtcg gtctgctttt tgctttgatc gttgtatgtt tacttgtttt gttcttacgt
    52501 acagaaacag gacttgctat tcgtgcaact ggcgataatc tggcaatgag cgaagcaaac
    52561 gggatcaaca cagataatat gaagatcatc ggttatatga tttctaatgg atgcattgct
    52621 ctttctggct ccttacttgc gcaaaacaac ggctttgctg atttaaattc tggtattgga
    52681 acgatcgtaa ttggacttgc ttctattatc atcgcagaag ttttgttacg taaccagcca
    52741 ctatttctcc gtctcttgac tgtcgtttta ggtgcggtca tttatcgttt tattttagcg
    52801 ttagtcttcg aactgaatgt tgaaccaagt gattcgaaac ttgcttctgc tttcgtgcta
    52861 gtgatctgtc tttctttccc gcagatccag caaaaattcc atttacctca aaaaaagaaa
    52921 ggcggtagtg tcaaatgaat gacgtcttaa cattggaaaa tctccatcat acctttgaaa
    52981 aaggaacgat caacgaaaat catgtcctga aaggcattaa tctttcctta gatcccggcg
    53041 acttcgtgac gatcatcggt ggaaatgggg ctggtaaatc tactttgctg aacagcatcg
    53101 ccggtacatt ttctgtcgat cagggtcaaa ttttattgaa tggaaaaaat atcacgaaaa
    53161 aatctgttgt cgaacgttcg aaatccatca gtcgcgtatt ccaagatcct aaactaggga
    53221 cagccgttcg tttaacggta gaagaaaatc ttgctcttgc tatgaaacga ggaaaaaaac
    53281 gcggcttctt ccgtggtgtc aaaccacaag accgaacatt tttcaaagac cagctggttc
    53341 ggctaaattt ggggttggaa aatcgtttat cctcagaaat cggtttgctg tctggaggac
    53401 aacgtcaagc tattacgtta ttgatggcca cattacagcg tccagaactg atcttattag
    53461 atgaacacac tgcagcgctt gaccctgcta cttccaaaac ggtgatggaa ttgacagaaa
    53521 agctgatcac tgagcagaat ctcactgctt ttatggtcac tcatgacatg gaagacgcga
    53581 tccgctatgg gagtcgactg atcatgctgc atcaaggaag gatcgtcgta gatatccagc
    53641 aagaggaaaa acagcagctc acagtgcccc aattattagc gttgttcaaa caaaacagcg
    53701 gcacggcatt aaaagacgat caagtacttt tatcttcata atcagaaaga ggatgggaca
    53761 aaaaatgaac gcccaccctc ttcttttttc tctttccttc tttcgttttt gctttatgta
    53821 tcaaccgttg tttcttcgaa aactattttg ttctctcctt cttcctgata gaatggatgg
    53881 gaaaggagct tgatgccatg acaacgaaag acaaggtact tcaattattg aaaaagacac
    53941 cagattttct ttccggagaa aagttggcac agcaactgga agtttcccgg acaagtattt
    54001 ggaaagccat caaagaattg gaaaaggcag gctatcgttt tgagcacggg cctacagggt
    54061 atcgttacct gccctctgac gtactagatg caaaagaaat ccagcaagag ctacggtcgt
    54121 ttattcctga tttgaaagta caaacgatgt tgacttctga atctacgatg aaagatgcca
    54181 aactggcagc agctcaagga gattctgttt cgaagttatt tgttgctgat acacaagaac
    54241 aagctcgtgg acgttttgga cggccttttt tcgctgaacc tggacgagga atctacatga
    54301 gtctcttgct ccatccagac aaacggtttg atgaattgcc tcaatatacg atcatcgcag
    54361 ccagtgcctg tgtgaaagcc attgaatctg taaccggtcg ttctgcttcg atcaaatggg
    54421 tcaatgatat ctacatggat ggaaaaaaag tctgtgggat tttatctgaa gcagtcagtg
    54481 atatggaaag cggcaggatc agtcatatca tcattgggat cgggcttaat ttttctgttc
    54541 ctcaaaatca ttttccagct gatctccaag aaaaagccat ctctgtcttt cccgacggac
    54601 aagctagtgt cacaaggaat cagctaatca gcaaaatctg gcaagaattg ttcacttatc
    54661 tttctcaccc agaaaaagcg ttggacttct ataaagaaaa atcattcgta ttgggaaaaa
    54721 ccgtgtcgtt caatcaaaaa ggaacgactt atgtcggtct tgcaaaagaa atcggtgatc
    54781 atggcgaact gatcgtccaa acaaatgaac agaagatgag tttgtcttct ggcgaaatca
    54841 gtctcacatc tatccgtacg caataattat cagatttcac aaaaggacac ctgatctcat
    54901 acagtgtcct tttttctgaa ttctcttcaa aagactctcc ttattctatc agctgacagt
    54961 ttctctatcc tctagaaaaa ggtattctaa aaatatctat aaagcatggt acactttctt
    55021 taaatgaatc aatgcaaaaa acaagagagt catcagttta cacgatcaaa agagatagca
    55081 ggccgcttcc tttttcaatc ttatagatag accatgggta acgataaaaa caaaaaagaa
    55141 cttgcgactt attttgccgt aagttctttt ttagctagtg ccttaatctc ttaatcccaa
    55201 caaacgtctc gcatagatca cctgaccaga gtgcatagca gcatcatcaa taatagaaac
    55261 gagacgcatt cctcgtgtga ctggtggtgt ccagttctcg tcaattactt catctagctc
    55321 ggaaggttcc agccttctta agtaagaaat agcaaaatca gcagctcctc gcaagtatcc
    55381 tagcagatct tctatttgat tagcttggat ctgctttgct tgttccagac tatgcttcca
    55441 atcttgttcg ttatctgcta tcgtcatcgg aaaccggctt tcccagtctt gactatgcca
    55501 aattggagaa gttcctgcta aatcagcaat ctggaaatct aattctcgag cggtatgcca
    55561 agccagccaa gtcattgatt tgatctgcgg ggctctatct tccactggaa aacgattggc
    55621 ctcctcaata gagacgcctt caaacaaacg ttcaaaccga tcgtatgccc gatccacact
    55681 ctcgcatagc atatctaaat aatccattta ttttcctcct ttttatttat cctacttact
    55741 ttttcctaat tctaggatac ctacttatat gttactacac acccttattt tttacacttt
    55801 aacagtcacc atcatcaata gtttggagat tgaaagaaaa agatagtgtc gaactatcaa
    55861 ttgaacaaga agcaacaata tgaacgtttc attaatattc tttaaataaa caatgatttt
    55921 tcaaacgaac agtttaagac taggagaaaa agagtataat aggaaaatgt ttgaaaggaa
    55981 aggaagggga aaaatgaaaa aaagggcaag tttactagtc ctagttgctt tgttgttcaa
    56041 tatcttttcg tatggtgtgg ccgtttcagc ggaaacatac attcctaaag accctgggaa
    56101 tatttataat gatctaactg gtaactatgg cttgttagga attgctagtc aattccacgt
    56161 atttgctaaa aataagacaa ctgtgaatgc acatacagac ggaaatatag caacacatga
    56221 acttgatgca aaaaataatt ttggtacaga gcttcatgaa ggtttattga accaagaaat
    56281 aaattatata caaaaaactg attctttcgc taattcttca ggcataccta caggtagcac
    56341 aagaatgaat aaatttgtcg ttggcagtaa cgtggatgta gcacttcaag atggtcatcc
    56401 tacagtcaat ggatccaggc ttgatcactt aactccagat gatttttata aagatcgtgc
    56461 tgggcatgaa tatattgatt tcgagcagga atttgctaaa ttgaatgtag cagcaaatga
    56521 tcttgcaacg atcactccag ctaaaacata tactgctgca gattttccag acatgaacaa
    56581 tcggacaatc gatttaactg atttatcaga taccgggttt ttattggtaa atatcgatgc
    56641 agaagtgtta acgatgaata cgccacttca aatcattaat ccaaatgatc aagtagttgt
    56701 atttaatgtc atcaacagtt cttctgcatt gaacgtacag tcaccaatca aatacaatga
    56761 tcgttcaaat catgaaacag aagatttttc ggatgcgaat atctcttgga attttggaaa
    56821 tgagatgact gatttgacaa tcagtgctcc ttttcaagga acaatccttg cgcctaatgc
    56881 tactatccgc attactcaaa accaagacgg aacgattata ggaacaaatg tgattttaga
    56941 tgcagctacc aatcgttggg atcctaacga aatatttatc agcgacaatg gtacagatac
    57001 gacagacaca agcgacagta cagatacatc taccacaagt gatagctcag attcatcaac
    57061 ttcaagcgac agtacagata ctacctctac cacaagtgat agcacggata catcagcttc
    57121 aagcgacagt acagatacta cctctaccac aagtgatagc acggacacat cagcttcaag
    57181 tgacagtaca gatactacct ctaccacaag tgacagcacg gacacgtcag cttcaagcga
    57241 cagtacagat actacctcta ccacaagtga tagcacggac acatcagctt caagtgacag
    57301 tacagatact acctctacca caagtgatag cacggacacg tcagcttcaa gcgacagtac
    57361 ggatacatct accacaacaa gtgatagcac ggacacgtca gcttcaagtg acagtacaga
    57421 tactacctct accacaagtg atagctcaga ttcatctacc actacaagtg atagcacgga
    57481 cacgtcagct tcaagtgaca gtacagatac atcctctacc acaagtgata gctcagattc
    57541 atcaacttca agcgatagta cagacacgtc gtctaccaca agcgatacac gtcatgcaag
    57601 tgtctccttc tcaaaaacaa gttcggataa caggacgaac tttaacttaa ctaataaaac
    57661 aagcaatgac aatggaaaca aatcaaaaag agcacttcct aaaactggaa gtcaatcgaa
    57721 taattggata acactcgcag gcgttatatt acttgtaatc ggtttaagaa tcacttttat
    57781 gagttataag aaatcgaaaa ggtaaaaaaa tattcaagat aaaaaagagc aacgtcgcta
    57841 acggacgttg ctctttattt ttgcttttta tcataaatac aagaactaca attcaatttg
    57901 tcaaaaaaca cgtagatttc cttctgagaa atttatttac gtatcgaacc tttccctatt
    57961 cagtctttgc gatccaaaat cagttgggta agggaaaaga gattctcttt tgttcgaaga
    58021 gaggtggcag gtaatttctt tatttccttc aaagcctttt ctgtgtaaga ggccgctaac
    58081 ttcctagctt cttccacgcc tcctgtatgt tcaacgattt tgcgtacggc gtcgacatct
    58141 tcttctgtca ttgccgcttt cttctctaaa taaggtagta gctttggttt gtttttttgc
    58201 aaagagtaga tcaacggtaa agaatagacc ccttgacgca tgtcttctag tacaggtttg
    58261 ccgatttcct ggctctcttt tgtataatct aaaatatcat cgataatctg aaaggccatc
    58321 ccgatagaaa gtccgatatc tcctgctttt tttgcgaagc gctcgcttgt tccactttca
    58381 aaagcgccaa cagaacaact taattggaac agttcagctg tcttacctga aatattttcc
    58441 aaatattctt ttacagaaag ttcataatta tagcgatcat ccatctgccc taattcgcca
    58501 ttcaatactt tttccatact gcgagaattc aattggagac ttttcaagga agtggagtaa
    58561 tcagacaata gtttaaaaca gcagacaaat aaatagtctc ctgcatagac agaaacgaca
    58621 ttaccaaact gactacggat attaggctga ccacgtcgca gatccgcttc atcaacgata
    58681 tcatcatgga tcagtgtggc cgtatggagc atttcgatag aagctgccaa agcgatggct
    58741 ttgtctcgat cttgctctgg tccaaattgt gagaacaata gctgataggc aggacgcaac
    58801 agctttccgc cggcatggat cgtttctttg atggcttgtt cgacaggttt gtttttcagg
    58861 cggacagttt cttccatccg ttttagtgtt tctgtcaatt ctttacttaa agcaggatag
    58921 ttttcccaca ttggatgtag cttcataaaa aaactccttg tgtgacgatt attttttggt
    58981 aaacattggg ctccattttt gtttggttcc ctgctcataa tctttttgga atcgttgaag
    59041 tgtctgcaca tgtcgtaaaa gataattcgc tgcgatcccg attgcaatcc ctgataaaat
    59101 ccctaaaact gaaaggactg gcaagtacaa catgactgtc catgattgtg caatgaaaga
    59161 agccatcaaa agctgtccga cattatgcat gaaaccgccg gccgcactaa ttccgatgat
    59221 actgacacgt tttggtccta attgcttgac caataacatt ccaaaataac taagcaaagc
    59281 accactcata ctataaagga atgtggaaat cgttccgcct agcaaagtag tcaggaatag
    59341 tcgtagccaa agtagtaaga agctgtcttg ttttttcatg gtaaaaatgg caacgatcgt
    59401 gattagattg gctaagccta gttttgcacc aggcgcaaaa gaaaaaggat aagggatcat
    59461 attttccaat aaaccaatga tcactccttg tgcaacaagt agcgaaatat aaatcatttt
    59521 ttgtaattta ctcatggtgt gtgaaggtct aataaatcag actgtcttcg tcacctccat
    59581 ctgttgaacg gacttcgatc actaatttat gtggcaaaca gacgatggtt tcgccatttt
    59641 tcgttgccca gcctcgacgt acgcagatct ggtcatcgca atctgcttct tttatccgta
    59701 ctcgatcgcc atctatctcg atcaagttat aatttccgtg ttcatctttg taagtatatg
    59761 tgttgctctc ttctccagcc atcaaaggga atgttttgat ttctgtgcca tccacacgca
    59821 gtaccgcttc tttttctgct gttgcggtgt tttgctgaag accaaaaatc atgactggta
    59881 aaaaagaaag aacagtcaaa agcaaaataa taaaaacatc ccaaggtttg agcagacttt
    59941 tttcagcaaa ttctttaagg ctcatttttg ttctcctttt tccagattaa ctcacccaat
    60001 tttagcataa aaacaagagg ttgtgacacc tgtgtcttgc tttgagtaat aagaacgaag
    60061 aatagaaaac agctttccat gttttctaat tctttgggct tattaccgag aagcagcagg
    60121 tggaacaccg tttataacaa gaggttgtga cagaagtggg caacttcaag aaataagaag
    60181 gaatttacga aaattacttt tcaagttttt gtgaaatttc ggcttatttt tcgaagaagc
    60241 tacttctgga acaccgttta taacaagagg ttgtaacaga ggttgtgaca cctgtgtctt
    60301 gctcatggag gcttatctcg ggcatcttat tcctttaatt ttacataaaa aggaggctgt
    60361 gacggtttgt ctcagcccct ttctattgaa atcttgcatt caaataacgt tctttatcta
    60421 tttatttctt gccgtactgg gaacttggtc taccgtacca ccaattacct agtgtacgtt
    60481 gtagccatgg aattacccag cgatccaacc caatcgcacg tccggaaccg ttcatcaatg
    60541 cgaaagcaac aaaaatgaac caaatgttta cccagtaaaa catgcctgat aagcagaacg
    60601 tgatcaccaa tacaatcgtt gtcgcactgc atagccatgt gaataatcca gcaatcaatg
    60661 ctaatgccaa ggcaatttca aagaatacca tgaatttttg gaagaacatt gctacatcat
    60721 tgtttggcat gatcacttgc atgatctttg tgaaccagtc tggtgacttc tcaatcacag
    60781 ccattggttc ttcaccgtat gcataactca aaccaaaatg tgcagcttga gtcgctgctt
    60841 ctccggcact tgcccctgct ccgctggcag ctgtggttgc atcaccagca gcagaagcac
    60901 cactggcaac atctgttaca ttgtattccc atgtccaagg gaacacactg tttccagtga
    60961 accatgatcc ttcaccaaac caagtacttg gtttgaacac ttcaccattc ccaatgattt
    61021 ttttggaagc ttcaacgaac caaactaaac catagaaaat acgaagtggc acactccata
    61081 agacattgcc gtaacgagaa gaatgtcctc gagtcacgtt gcgatcatct ttgatatgga
    61141 aaaactcatg cattaaatat tggaacatat aataaccaga acgaatatca aagaagtatt
    61201 tcaaattcac gatatgtttc attacgatag ctaaaaatcc gcttaaatgg atcttatcaa
    61261 ataggttagc taccccccat ttggcaccaa tagaaaccat aaatccttga tagtttcctt
    61321 taaatttatg cttttctccg cctttgatcg aagccacgat attcgctgcg gctgtgtgtc
    61381 ctgtttgttc agcagcttga acgatctgcg gtgtcggtgt tcctgggaat tcttcataaa
    61441 agactaaatc accaatgata tagatgtttt tatcttcgta gccttttgcc tgcatatatt
    61501 cattggcgat caaccgctgt ccgcgtgctg attctaatcc aaagtcagca gcgtcagaag
    61561 ttcctttgac accagctgtc caaatcagtg tgtgagtcgg aatgttgcgt ccgtctttca
    61621 atttgatatg atcagcagcc acttctacga ttggtgcatt caataataat tccacgttgt
    61681 gcttacgcaa atagcgttca gcttttgcag catcatttcg atccaacata ttcaagatcg
    61741 tcggcattgc ctctacgacc atcagtgtga tttcgtttgg atctaattta taatctttgg
    61801 ctaggcgatt tttccagtcg atcagttccc caatcatttc aataccggta aatccagaac
    61861 cacagacaac gaaagtcagc atggctttac gcagttcatc atctggttcc aatgcagctt
    61921 ttgctactgt cttctcaata tgttcccgaa tccttacaga atcttcaaat gaccaaaggg
    61981 tgaatccgtg tgctttgaca ccaggtgtac caaagtcatt cggctctccg cccattccaa
    62041 ggatcagctg atcaaaagga taggaaccta atttggtctt gacgattttt cgttctttat
    62101 cgataccgat tacagtgtct gtgacaagtt gtacattctt ttgacgagca aacaaatgct
    62161 gcagatcgta ttggattgct tctggttcta cacgtccgcc agctacttca tgcaactcgg
    62221 tcatcatagt atggtacgaa tgacgatcga tcagagtaat tgctacatct tcttttttca
    62281 gtttctttgc caagtatttt gtagctgaaa caccggcata tcctgctcct acaacgacaa
    62341 tattttgctt tgccattgat catccgctcc ttatagacta tctatataat gaatatatga
    62401 taattctttt agtttgatac ttattacaca atctccatat attatataga tgttctgttc
    62461 ttttgtctaa ctccttttgg atttttttca aattttaaaa taaagcgttt tttcacgaaa
    62521 tttgctgaaa gaaaacgttt gaaatattac atgggttatt tatcaaaaat agttgactta
    62581 taggctaaaa agattagaat gtataatgaa tcacaaagaa aaattgtgga ctagaaatgg
    62641 aatggtgggg tattaaaatg aagaaaaaac gttttgtaac tggatttgcc gtattagcat
    62701 tttcagctct agtgttaggc gcttgcggcg cagatgacaa cggaagctca aactcttctt
    62761 ctgaatcatc tacagcacaa tcttcgactg caaaaagcac aacagaatca tctgcaaaag
    62821 ttgtagctgg cggagatttg caagacggca catataaatt agaagaaaaa aattattcaa
    62881 atggttaccg tgcagtcttt gaaatggttg taaaagacgg caaaatcact gaatcaaaat
    62941 atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga tgcagaatac aacaaaaaca
    63001 tggaagcaaa atctggcaca aacccagaaa aatacattcc tgagttgaat gaccaattct
    63061 taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg agctactcac tcatctgaaa
    63121 gcttccaaaa ctacgcacaa caattgatcc aagctgctca agctggtaac acagatacaa
    63181 tcgaaatcga caatggtgct gatttgaaag acggcactta caagcttgaa gaaaagaact
    63241 actctaacgg ctaccgtgtg caattcgaaa tgacagttgc tggcggcaaa gtaactgaat
    63301 caaactttga ttatatcgac aaagacggta aatcaaaaca agatgataca gaatataatg
    63361 aaaatatgaa agcaaaaagc ggtacagaac caaaaactta catccctact ctgaacgacg
    63421 aattcgtcaa agcaatgggc gaagaagatg gctcacctgc taatgtagaa gttgttacag
    63481 gtgctacaca cagctcacat tcattcatca tgtacgcaca acaattagtg aatgcagctg
    63541 aaaaaggcga cactcaaaca atcgaagtag ataatatcgt aacagaaaaa taatcacata
    63601 atcatactaa taaaagttgg agggtgggac gatagccaga ttaggcagtc gtctcacctt
    63661 tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta ttttccttaa tcacactggg
    63721 catctctttt atggtgctaa ctggctgcag caactcagca aataaaacaa ccactgaaag
    63781 ttcagcagct accaagatat tgaaagatcc atattctgat gaacagttct tgttagggac
    63841 ttacgttcgt atccgtgtgt atgatgaagg caaagaatca gcattgaaac ctgccttcga
    63901 tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa aaaggttctg agatcgatga
    63961 agtgaatgag caagctggga tcaaaccagt aaaagtttct gatgacgtct acacgttagt
    64021 caagcgggcc tacgaatata gccaagattc tcaaggcgga ttcgatatgg cgattggtgc
    64081 aatcacacaa ttatggcgaa tcggttttga tgacgccaga aaaccttccc aagaagaaat
    64141 cgatcaggca ttgaaattag tggattatca taagatcgaa ctaaatgaca aagaaaaaac
    64201 tgtttatcta aaagaaaaag gaatgatcat cgatttaggt gcaatcgcca aaggttacat
    64261 tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg accactgcca tcgttgactt
    64321 gggcggtaat gtctatgtct taggccacag cccaagaggc gaaaaccaag actggacagt
    64381 cggcatccaa gatccgaaca tggctcgcgg atctgtttta ggttctatta aagaacggaa
    64441 caaaacatta gttacctctg gaatttacga gcgttattta gaagtagatg gcaaaaaata
    64501 ccatcatttg tttgatccaa aaacgggtta tccgttcgac aatgatatcg caagtgtcac
    64561 gatcataaca gataaatcaa ttgatggtga cgggttgtct acagcagtct tttcaatggg
    64621 tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat aacgggacgg aagcgatctt
    64681 cgtgacaaaa gacgataaag tatatgtgac cgatccaatc aaagacacct tcaaattatc
    64741 ggaggactcc cattacacga tgggtgaccg ttctgaacta aaataaggag tacgaaccat
    64801 gacactaaaa atctttttag aagtagttga aatacgaaca aaggtggcta gtgtatttcc
    64861 ttttgcggta ggggtattat tctctatcgc tttttttcat gagatacatt ggctaaacac
    64921 ttttttattt tttattggaa tgctagtttt tgatttagcg actaccgcta tcaataa
//
LOCUS       DAJSBN010000009        62714 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJSBN010000009 DAJSBN010000000
VERSION     DAJSBN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.009"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ONT86_000659"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ONT86_000659"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR2351475.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ONT86_000660"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ONT86_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR2351476.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ONT86_000661"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ONT86_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR2351477.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ONT86_000662"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ONT86_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR2351478.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ONT86_000663"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ONT86_000663"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR2351479.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ONT86_000664"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ONT86_000664"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR2351480.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ONT86_000665"
     CDS             complement(8053..8523)
                     /locus_tag="ONT86_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCR2351481.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ONT86_000666"
     CDS             complement(8569..8994)
                     /locus_tag="ONT86_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCR2351482.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ONT86_000667"
     CDS             complement(9061..9330)
                     /locus_tag="ONT86_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCR2351483.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ONT86_000668"
     CDS             complement(9341..10936)
                     /locus_tag="ONT86_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2351484.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ONT86_000669"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ONT86_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR2351485.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="ONT86_000670"
     CDS             complement(14489..15049)
                     /locus_tag="ONT86_000670"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR2351486.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ONT86_000671"
     CDS             15402..16376
                     /locus_tag="ONT86_000671"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR2351487.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ONT86_000672"
     CDS             16546..17946
                     /locus_tag="ONT86_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCR2351488.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ONT86_000673"
     CDS             complement(18024..18746)
                     /locus_tag="ONT86_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2351489.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ONT86_000674"
     CDS             complement(18825..19331)
                     /locus_tag="ONT86_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR2351490.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ONT86_000675"
     CDS             19454..21253
                     /locus_tag="ONT86_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCR2351491.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ONT86_000676"
     CDS             complement(21321..22895)
                     /locus_tag="ONT86_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR2351492.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ONT86_000677"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ONT86_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR2351493.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ONT86_000678"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ONT86_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR2351494.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ONT86_000679"
     CDS             complement(24672..25310)
                     /locus_tag="ONT86_000679"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCR2351495.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="ONT86_000680"
     CDS             complement(25311..25961)
                     /locus_tag="ONT86_000680"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR2351496.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ONT86_000681"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ONT86_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCR2351497.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ONT86_000682"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ONT86_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCR2351498.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ONT86_000683"
     CDS             complement(29104..29844)
                     /locus_tag="ONT86_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCR2351499.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ONT86_000684"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ONT86_000684"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR2351500.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="ONT86_000685"
     CDS             complement(31215..32153)
                     /locus_tag="ONT86_000685"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR2351501.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ONT86_000686"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ONT86_000686"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR2351502.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ONT86_000687"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ONT86_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR2351503.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="ONT86_000688"
     CDS             complement(35313..35618)
                     /locus_tag="ONT86_000688"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR2351504.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ONT86_000689"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ONT86_000689"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR2351505.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="ONT86_000690"
     CDS             complement(36724..37008)
                     /locus_tag="ONT86_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351506.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="ONT86_000691"
     CDS             complement(37026..37916)
                     /locus_tag="ONT86_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR2351507.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="ONT86_000692"
     CDS             complement(37936..38154)
                     /locus_tag="ONT86_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCR2351508.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="ONT86_000693"
     CDS             complement(38124..39824)
                     /locus_tag="ONT86_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR2351509.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="ONT86_000694"
     CDS             40091..40864
                     /locus_tag="ONT86_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351510.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ONT86_000695"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ONT86_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCR2351511.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="ONT86_000696"
     CDS             complement(41688..42659)
                     /locus_tag="ONT86_000696"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR2351512.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ONT86_000697"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ONT86_000697"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR2351513.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ONT86_000698"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ONT86_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCR2351514.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="ONT86_000699"
     CDS             complement(45614..46438)
                     /locus_tag="ONT86_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR2351515.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="ONT86_000700"
     CDS             complement(46411..47091)
                     /locus_tag="ONT86_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR2351516.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="ONT86_000701"
     CDS             complement(47114..48052)
                     /locus_tag="ONT86_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCR2351517.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49160)
                     /locus_tag="ONT86_000702"
     CDS             complement(48297..49160)
                     /locus_tag="ONT86_000702"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR2351518.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ONT86_000703"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ONT86_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR2351519.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="ONT86_000704"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="ONT86_000704"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR2351520.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ONT86_000705"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ONT86_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCR2351521.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ONT86_000706"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ONT86_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR2351522.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="ONT86_000707"
     CDS             complement(56026..57267)
                     /locus_tag="ONT86_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCR2351523.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ONT86_000708"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ONT86_000708"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR2351524.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59217)
                     /locus_tag="ONT86_000709"
     CDS             complement(58294..59217)
                     /locus_tag="ONT86_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2351525.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="ONT86_000710"
     CDS             complement(59956..61242)
                     /locus_tag="ONT86_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR2351526.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="ONT86_000711"
     CDS             complement(61235..62503)
                     /locus_tag="ONT86_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR2351527.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
BASE COUNT        17307 a        13635 c        11225 g        20547 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttt
//
LOCUS       DAJSBN010000010        61365 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJSBN010000010 DAJSBN010000000
VERSION     DAJSBN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.010"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..561)
                     /locus_tag="ONT86_000712"
     CDS             complement(<1..561)
                     /locus_tag="ONT86_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCR2351528.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(748..1398)
                     /locus_tag="ONT86_000713"
     CDS             complement(748..1398)
                     /locus_tag="ONT86_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351529.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="ONT86_000714"
     CDS             complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="ONT86_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCR2351530.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2910..3908)
                     /locus_tag="ONT86_000715"
     CDS             complement(2910..3908)
                     /locus_tag="ONT86_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCR2351531.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="ONT86_000716"
     CDS             complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="ONT86_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR2351532.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4924..5655)
                     /locus_tag="ONT86_000717"
     CDS             complement(4924..5655)
                     /locus_tag="ONT86_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCR2351533.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5659..6924)
                     /locus_tag="ONT86_000718"
     CDS             complement(5659..6924)
                     /locus_tag="ONT86_000718"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCR2351534.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="ONT86_000719"
     CDS             complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="ONT86_000719"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR2351535.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="ONT86_000720"
     CDS             complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="ONT86_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCR2351536.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12266..13768
                     /locus_tag="ONT86_000721"
     CDS             12266..13768
                     /locus_tag="ONT86_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCR2351537.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13770..14507
                     /locus_tag="ONT86_000722"
     CDS             13770..14507
                     /locus_tag="ONT86_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2351538.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14552..14725)
                     /locus_tag="ONT86_000723"
     CDS             complement(14552..14725)
                     /locus_tag="ONT86_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351539.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14701..15366
                     /locus_tag="ONT86_000724"
     CDS             14701..15366
                     /locus_tag="ONT86_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2351540.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="ONT86_000725"
     CDS             complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="ONT86_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR2351541.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16765..18594)
                     /gene="dnaK"
                     /locus_tag="ONT86_000726"
     CDS             complement(16765..18594)
                     /gene="dnaK"
                     /locus_tag="ONT86_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR2351542.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18645..19208)
                     /gene="grpE"
                     /locus_tag="ONT86_000727"
     CDS             complement(18645..19208)
                     /gene="grpE"
                     /locus_tag="ONT86_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR2351543.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19302..20345)
                     /gene="hrcA"
                     /locus_tag="ONT86_000728"
     CDS             complement(19302..20345)
                     /gene="hrcA"
                     /locus_tag="ONT86_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCR2351544.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20579..21745)
                     /locus_tag="ONT86_000729"
     CDS             complement(20579..21745)
                     /locus_tag="ONT86_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR2351545.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21761..21988)
                     /locus_tag="ONT86_000730"
     CDS             complement(21761..21988)
                     /locus_tag="ONT86_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR2351546.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22472..23062
                     /locus_tag="ONT86_000731"
     CDS             22472..23062
                     /locus_tag="ONT86_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCR2351547.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23252..23965
                     /locus_tag="ONT86_000732"
     CDS             23252..23965
                     /locus_tag="ONT86_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351548.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24080..25090)
                     /locus_tag="ONT86_000733"
     CDS             complement(24080..25090)
                     /locus_tag="ONT86_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCR2351549.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25127..25609)
                     /locus_tag="ONT86_000734"
     CDS             complement(25127..25609)
                     /locus_tag="ONT86_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCR2351550.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25759..26127
                     /locus_tag="ONT86_000735"
     CDS             25759..26127
                     /locus_tag="ONT86_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCR2351551.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26592..27044
                     /locus_tag="ONT86_000736"
     CDS             26592..27044
                     /locus_tag="ONT86_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2351552.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27168..28019)
                     /locus_tag="ONT86_000737"
     CDS             complement(27168..28019)
                     /locus_tag="ONT86_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCR2351553.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28032..28817)
                     /locus_tag="ONT86_000738"
     CDS             complement(28032..28817)
                     /locus_tag="ONT86_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2351554.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29169..30110)
                     /gene="ribF"
                     /locus_tag="ONT86_000739"
     CDS             complement(29169..30110)
                     /gene="ribF"
                     /locus_tag="ONT86_000739"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCR2351555.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30114..31037)
                     /gene="truB"
                     /locus_tag="ONT86_000740"
     CDS             complement(30114..31037)
                     /gene="truB"
                     /locus_tag="ONT86_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR2351556.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31405..33767)
                     /locus_tag="ONT86_000741"
                     /pseudo
     CDS             complement(31405..33767)
                     /locus_tag="ONT86_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34215..34388
                     /locus_tag="ONT86_000742"
     CDS             34215..34388
                     /locus_tag="ONT86_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2351557.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34545..34892)
                     /gene="rbfA"
                     /locus_tag="ONT86_000743"
     CDS             complement(34545..34892)
                     /gene="rbfA"
                     /locus_tag="ONT86_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR2351558.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34919..37225)
                     /gene="infB"
                     /locus_tag="ONT86_000744"
     CDS             complement(34919..37225)
                     /gene="infB"
                     /locus_tag="ONT86_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR2351559.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37238..37549)
                     /locus_tag="ONT86_000745"
     CDS             complement(37238..37549)
                     /locus_tag="ONT86_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCR2351560.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37546..37839)
                     /locus_tag="ONT86_000746"
     CDS             complement(37546..37839)
                     /locus_tag="ONT86_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCR2351561.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37861..39036)
                     /gene="nusA"
                     /locus_tag="ONT86_000747"
     CDS             complement(37861..39036)
                     /gene="nusA"
                     /locus_tag="ONT86_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR2351562.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39059..39532)
                     /gene="rimP"
                     /locus_tag="ONT86_000748"
     CDS             complement(39059..39532)
                     /gene="rimP"
                     /locus_tag="ONT86_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR2351563.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39677..44035)
                     /locus_tag="ONT86_000749"
     CDS             complement(39677..44035)
                     /locus_tag="ONT86_000749"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCR2351564.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44247..45956)
                     /locus_tag="ONT86_000750"
     CDS             complement(44247..45956)
                     /locus_tag="ONT86_000750"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR2351565.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46024..47292)
                     /gene="rseP"
                     /locus_tag="ONT86_000751"
     CDS             complement(46024..47292)
                     /gene="rseP"
                     /locus_tag="ONT86_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCR2351566.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47453..48253)
                     /locus_tag="ONT86_000752"
     CDS             complement(47453..48253)
                     /locus_tag="ONT86_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR2351567.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48250..49062)
                     /locus_tag="ONT86_000753"
     CDS             complement(48250..49062)
                     /locus_tag="ONT86_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCR2351568.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49257..49814)
                     /gene="frr"
                     /locus_tag="ONT86_000754"
     CDS             complement(49257..49814)
                     /gene="frr"
                     /locus_tag="ONT86_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR2351569.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49817..50539)
                     /locus_tag="ONT86_000755"
     CDS             complement(49817..50539)
                     /locus_tag="ONT86_000755"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR2351570.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50675..51556)
                     /locus_tag="ONT86_000756"
     CDS             complement(50675..51556)
                     /locus_tag="ONT86_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR2351571.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51655..52437)
                     /gene="rpsB"
                     /locus_tag="ONT86_000757"
     CDS             complement(51655..52437)
                     /gene="rpsB"
                     /locus_tag="ONT86_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR2351572.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52796..53275
                     /locus_tag="ONT86_000758"
     CDS             52796..53275
                     /locus_tag="ONT86_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCR2351573.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53492..54583
                     /gene="phnW"
                     /locus_tag="ONT86_000759"
     CDS             53492..54583
                     /gene="phnW"
                     /locus_tag="ONT86_000759"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCR2351574.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54576..54704
                     /locus_tag="ONT86_000760"
     CDS             54576..54704
                     /locus_tag="ONT86_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR2351575.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54708..55253
                     /locus_tag="ONT86_000761"
     CDS             54708..55253
                     /locus_tag="ONT86_000761"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR2351576.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55707..57398)
                     /locus_tag="ONT86_000762"
     CDS             complement(55707..57398)
                     /locus_tag="ONT86_000762"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR2351577.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57818..58765)
                     /gene="arcC"
                     /locus_tag="ONT86_000763"
     CDS             complement(57818..58765)
                     /gene="arcC"
                     /locus_tag="ONT86_000763"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR2351578.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58880..59899)
                     /gene="argF"
                     /locus_tag="ONT86_000764"
     CDS             complement(58880..59899)
                     /gene="argF"
                     /locus_tag="ONT86_000764"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR2351579.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59990..61219)
                     /gene="arcA"
                     /locus_tag="ONT86_000765"
     CDS             complement(59990..61219)
                     /gene="arcA"
                     /locus_tag="ONT86_000765"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCR2351580.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT        17058 a        13102 c        10624 g        20581 t
ORIGIN      
        1 tataaacatc accccaaata aaacagaaaa cagtataagc attataaaaa ttatcaacaa
       61 ccttgatcct tcttttatag attcaggaaa cagtataatt ttagtactag ataaattgtt
      121 tacaccgatt agtgttaaaa tatctttatt cttcttgcta acataaccct ttatgcacaa
      181 aagaacagct atacttaaag taacaaataa ataacgtatt ccaatagaaa aatcactata
      241 aatactctct agcataggac cgacaattga agaagtcact actgcaaatg ctggacctat
      301 ccaagcaaca ttagaatgtt ctttctctct tttcctttgt aaatctttgt tgagtattaa
      361 tctatttatt tcttctgcag gcaagttaat tttcataacc ttatggggga ttatccaatt
      421 aaaaatcgga aaaaggtagc ctccccaaaa agtatagtga tctgtgtcaa tcagataata
      481 acctgatttt gattccagaa taacatatct caaagccgaa cgaaaatttt tcatgaatat
      541 ccataatctc actgtttcca cacctaattc ctccaattcc aattaaatgg atttatagca
      601 tcaccaatta tgttaactgc ctgaccaacc tcattgataa aattggcacc actatcccat
      661 gcttcaacaa tgctatatat ctatcaaaca aaaaatggaa aactaaatac tcgcgcacat
      721 aaattttaca ttctgttttt tataagacta ataataatca ttgaaaaaat ttcttatcgg
      781 tggcgcatat tcccaattga ttaatgtgat cccatattct gcaagtattt tacgagcttc
      841 ttctaagtca tagtgagaaa aatgcataaa acctgcaata cgatcacgga ctgtgagctg
      901 tggcttgctt ccaccatcat tcataaaata tccagcagag ccactactta aagtgattgt
      961 tacatcttca ctttctatta ttaagaattc ataatgaaat gctttcaact ctccactatt
     1021 atttaaaagt tttatactcc aacttcgtct aggttgagtt gtcacattcc ctctaaaact
     1081 tcctataaat tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata
     1141 ttcaagacta aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct
     1201 ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag
     1261 acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag
     1321 aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg
     1381 ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca
     1441 ggtataacgt ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg
     1501 aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga
     1561 ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca
     1621 gaagtaagaa aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc
     1681 tccagttata gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt
     1741 ttatgtataa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
     1801 aaaaacaata gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga
     1861 cggaagaaaa gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat
     1921 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     1981 attttgcgaa ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc
     2041 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2101 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2161 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2221 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2281 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag
     2341 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     2401 tcttggttat gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga
     2461 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     2521 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     2581 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     2641 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     2701 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     2761 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     2821 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     2881 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     2941 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag
     3001 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3061 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3121 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3181 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3241 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3301 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3361 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     3421 atggttttac ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa
     3481 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     3541 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     3601 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     3661 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     3721 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct
     3781 gcaacagtca ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact
     3841 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     3901 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     3961 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4021 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4081 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4141 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4201 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4261 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4321 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg
     4381 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     4441 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     4501 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     4561 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca
     4621 tttttgtgac tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg
     4681 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     4741 ctgcaccact catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc
     4801 cctctcacat tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt
     4861 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     4921 tttttacttt tccgatgctg tatccaatgc ctcgcttctt tcagctaatg cacgttcgat
     4981 cgttcgatcc gctaaaatag attgagcatc gatcactggg taatgattat cttcagcaaa
     5041 ttcatgcatc aaagataatt ctgtgcaacc taggatgact ttttcacagt ttaggcgctc
     5101 taccgcttcc tctaagattt cgtaatagag ctcttgattc aaataatccg attccttgat
     5161 ctcatgatag atcaggtaat tgattttttc ttgaagcgca gtatcaggaa tcactgtttc
     5221 gaaaccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5281 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5341 catatgcaag ataggaatat ccgttgccgc ttgtaactct tcaaaaaagt agtgtgcggt
     5401 gttacaagtc aacacaatga agtttggtcg caataaattt tgtttctcaa tatcatccag
     5461 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     5521 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     5581 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     5641 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     5701 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgatcggt ctttttcata
     5761 aaatacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     5821 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     5881 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttgaaca ctcgatcttc
     5941 tgtgacaaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6001 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6061 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6121 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6181 taatagtgga tgaccaaggc acatcatccg aacttgatga tcttcatcta catacgcatt
     6241 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6301 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6361 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     6421 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     6481 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     6541 gcttacttta ttgatcagcc tttcaaataa agaataatca atatatggac agataaaata
     6601 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
     6661 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
     6721 gataaataca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
     6781 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
     6841 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
     6901 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
     6961 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aagtcctccg
     7021 attgctcaat tcttttttga acggattgaa cttataggaa gtttttatct ataccaatca
     7081 gtgtagaaag aactaccatg taatgattct agaacactga tattaataaa acaagcaaaa
     7141 attcattttt ttatgttttt ttaactatct cagctatcta tcctctttat accattattt
     7201 ttttcaaata gtgacctgta taactttctt ttacgttaat gatttcttct ggtgttccag
     7261 tcgcaaggat cgttcctcca ccttctccac cttctggtcc taaatcaatg atataatctg
     7321 ctgttttgat tacatctaga ttgtgttcaa tcactaaaac tgtgttacca gcatctacta
     7381 atctttgtaa tacatgcaac aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt
     7441 catctagtat atagaaattc tttccattag agattttgtg caattcactg gcaagtttca
     7501 tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc acataaccta
     7561 agccaacatc aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt
     7621 ctacagcatc ctctactgtc atttccaaaa tatcagcaat gctttttcct ttgtaatgca
     7681 cttctaatgt ttcagaatta tatcgtttac catgacatac ttcacaagga acatagacat
     7741 caggcaaaaa gtgcatttcg atcttaataa ttccatcccc gcgacaagct tcacaacgac
     7801 cgccttttac gttgaaacta aaacgccctt tcttataacc ccgcattttt gcctcgttcg
     7861 tttgagcaaa taacccacgg atatcatcaa atacacttgt ataagtcgct ggattacttc
     7921 tcggcgtccg gccgattggg ctttgatcga tatcgatgat cttttcgata ctttcgtagc
     7981 cggaaattgt cttgaattta cctggcttag cagaattctt atttaatttt tgcgctaacg
     8041 atttctttaa gatactattg accaatgtac tctttccaga accagacaca cctgtgactg
     8101 ctacaaattc tcctaatgga aattcaacac taatattttt cagattgttt tctgaagcac
     8161 cagtgatctt gattgctttc ccattacctt tacggcgttc ttttggtaca gggattgatt
     8221 ttttccctga aagatatttc cctgtcaaag aattttcatc attagctact tcttcaggag
     8281 tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc aggtccgacg tcaatcaaat
     8341 aatctgctgc ccacatcgta tcttcatcat gttcgactac aacgagggtg ttgccaagat
     8401 cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg
     8461 atggttcatc taaaatatac aaaactccag aaagattcga tccaatttgt gtagccaatc
     8521 tgattcgctg cgcttctccc ccagataatg ttccggaagc gcgactcaaa gttagatagt
     8581 ctagtcccac attttccaag aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg
     8641 caattacttt ctcttgttca gataacgtta agtttgagaa gaaatctact gctttttgaa
     8701 tagacaactc atttgtttgc ccgatattcg ttccattgat ttgaacagat agagcttggg
     8761 gattcaatcg ataaccttta catgtctgac aagttaattc tgtcatataa agacgcattt
     8821 gctcacgcgt gaaatcactg ttcgtatcat gataacgacg ctcgatattc ttcaataccc
     8881 cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc attttcataa tggaaatgga
     8941 aaagatcatc tgtcccattc aagatgatct cctgatgctc ttccggtaat tcttcgaatg
     9001 gtgtatctaa atcgataccg aaactttcac aagcctgcgc taacatttga ggataatact
     9061 gagagctgat tggattccat ggaacgatag caccttgact aagtgtcttc gttctatctg
     9121 gaatcactaa atcttgatcg acttctaatt tgacacctaa accatcacag tctggacaag
     9181 ctccaaaagg cgcattgaat gagaacagtc ttggttctag ttcgccaaca gtaaatccgc
     9241 aataaggaca ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc acgtcaacca
     9301 gagcatagcc gtctgctaat cgcagggccg cctctaaaga atcaaatagg cgagaacgaa
     9361 tcccttcttt cacaatgatc cggtcgatga cgatcgcgat atcatgtttt ttgtttttct
     9421 ccagctcagg cacttcactt aaatcataga tctcaccatc gactcgaacg cggacgtatc
     9481 cctctttttg gattcgttcg aatatttttt tatgttgtcc ttttttcttt accacgatcg
     9541 gtgccaacac ttggatcttt gtgcgctcag gcaaagccat tacttgatct gccatttgat
     9601 ctactgattg actggtaatc tcgatatgat cgttgggaca aatcggatgt cctacacgtg
     9661 caaacaacaa gcgtaaatag tcattgattt ccgtcacagt acctactgtc gaccgaggat
     9721 ttttacttgt tgttttctga tcaatagaaa tcgccggact caatccgtcg atgctatcta
     9781 cgtccggctt atccatttgt cctaaaaact gacgagcata agcagaaagg ctctctacgt
     9841 atctccgctg cccttcagcg tacagcgtat caaatgctag ggaacttttt ccagaaccgg
     9901 acaaacctgt aacgaccacg aatttatctc gtggaatcgt tacatctata tttttcaagt
     9961 tatgggcacg tgcaccatga atcgtaattt tatcgttagc catgttgtcc tcctactttg
    10021 aagcttttaa ttctaaaatc atatctcgca cattcgcggc tgtttcaaaa tccaaagctt
    10081 ttgccgcatc tttcatttct ttttctaatt tcatcaataa cgtgtctttt tcttccttcg
    10141 tcatttcttc gtacgagact tgtaccgctt cttttgtatc atcttccgat tctttcgtaa
    10201 tagaaatcaa atctcggatt tctttaatga tcgtttttgg aacgatccca tgttcttcat
    10261 tgtacttctg ctgaatcgta cgacgtcttg acgtttcatc catcgctaga cgcatagaat
    10321 ctgttacttt gtctgcgtac atgattactt taccttcttc attccgagca gctcgtccaa
    10381 tcgtttggac taaggaacgt tcactacgta aaaagccttc tttatccgca tccaagatag
    10441 caacaaggga aacttcaggt acgtctaatc cttcacgaag caaattgatt ccaactaaca
    10501 catcaaattc acctaatcga agatctcgaa tgatttctgt tcgttccagc gttttgatat
    10561 cactatgcaa atatttgact ttgatcccta attctttgaa ataatctgtg aggtcttctg
    10621 ccattttttt cgtcaatgtc gttacaaaca cccgctggtc tttttcgact cgttcattta
    10681 tttcgcctac tagatcatcg atttgtccca tgattggacg aacttcgatc actgggtcaa
    10741 gcagccctgt cggacggatg atttgttgga taacggtatc tgtttgttca tgttcatatg
    10801 gtcctggcgt cgcagagaca taaatgatct gattgacatg tttttcaaac tcttctaacc
    10861 gaagtggccg attgtctaaa gcagatggca aacggaaacc gtaatctact agcatctgtt
    10921 ttctcgctcg gtcaccatta tacattcctc tgatttgagg catggttacg tgcgattcgt
    10981 ccgccacgat caaaaaatct tcaggaaaga aatccaatag cgtataaggc ggttcacctt
    11041 ctttacgtcc gtccatatgc cgagaataat tttcaattcc cgacgtgtag cccatttcta
    11101 acatcatttc aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc agaagtttgt
    11161 tttcatttcg caagacagtt aaacgctgtt ctaattccgc tttgatattc gctacggcat
    11221 gctccatatg ttcatcattc gtcacaaagt gagtcgccgg gaagattgct acgtgttccg
    11281 tttctcctaa aacttcacct gtcagtgcat tgacttctcg gatacgttcg acctcatctc
    11341 caaaaaactc cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc tccacgacat
    11401 caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc atttctttcg aattggatac
    11461 tgactaagtc acgaatcaac tgattgcgat ctagttctgc tccctgtcga atcgaaacta
    11521 cttgtttttg gtactcaaaa ggcgagccta atccaaaaat acatgaaacg gatgcaatga
    11581 cgatcacatc atttctttct aataatgaac tagtagccga atgtcgtaat ttgtcgatct
    11641 catcatttac acttgaatct ttttcgatat acgtatcgct tgaaggcaca taagcttcag
    11701 gctgataata atcataataa gaaacaaaat attcaacagc attgttaggg aaaaattcct
    11761 tgaattcccc gtacagctga ccagccaatg ttttgttatg ggcaataatc agtgttggtt
    11821 tattcacttt ctcaataaga ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta
    11881 ataagatttg tgcttttttc ccgccgacta cgccatccac taattggtta atcgcttcag
    11941 gctgatcacc agccggttgg tatttcgaga caagctcaaa gtgtcgggag gtatctctct
    12001 caatcattct tcgtcctcct tttcattcat tccttttcat tttacgacca ataaaaagaa
    12061 aaggaaagca aaggcattcc ttactaaaaa attataccac accgaacata ctttcgccac
    12121 ttttttatct tattatacat gttatgtttt ataacataaa atgttagaaa ctcgcatttt
    12181 tttcattttt tcgagcttac agtcttgtct gattcagcac ttttcgccta taataataaa
    12241 atattggaaa tttaggaggt tgattatgaa aagaaagcat ttatttcact catttgtttt
    12301 cttaatgaca ggacttgtga catttgcgtt tggtcatgtt gcacaagctg acaacactac
    12361 aacggcagag tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa aagacgtata
    12421 cgtaattgcc agtgactctg cttttgctcc atttgaattt caaaacgcaa agggcgagta
    12481 cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg
    12541 aatggactac cgtggtttct ctgctgcttt gcaagctttg gaatcaggac aagcagacgg
    12601 aatgattgcc ggaatgactg taacagatga acgaaagaaa agctatgatt tttctgattc
    12661 ttactttgat agcgggatcc aattagctgt caaaaaagga gatacttcga tcaagtctta
    12721 tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg ctgaattttt
    12781 aagcgaccat gaatctgaat atggctattc tatcaaatat tttgatgctg ctgatcaatt
    12841 gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg
    12901 ttatgcgatc aaacaaggac aagaattgga aacaccgatc aaaaaagaat ctggcggaaa
    12961 atatgcattt gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg
    13021 attgaaagaa ttaaaaagaa caggcgaata cgataagatt attaaagaat atgtagcgga
    13081 tggttcagaa acttccagtg agtcttccac tgctgatgaa tcaacatttc ttggacttat
    13141 ccaaaacaac tatcaagcat tgttgaaagg actttggaga acaattctcc taactttgat
    13201 ctcctttgct ttggctatag taatcggtgt gatttttgga ttattcagtg tagcaccaat
    13261 acgcgctctt cgcacaattg ctagtattta tgtcgatatc atccgaggaa ttccaatgat
    13321 ggttcttgcg ttctttattt tctttgggtt gcctggtatc gttggattta cgattcctga
    13381 cttccttgct ggtgttatca ccttgactct taacgcaagt gcttatatcg cagagattgt
    13441 tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct
    13501 ttcttataat cggacaatgc aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc
    13561 atcattcgtc aatcagttcg ttatctcatt aaaagacaca acgatcattt ccgcaatcgg
    13621 tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca cgtacaacac aaagtacgta
    13681 tgtttacttg atcattgcga tcatgtactt gattttgatc acagcattga caaaattagc
    13741 aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt gagcatttag
    13801 tcaaaaaata cggcgataac acagtattaa acgacatcaa tgtatccatc aacgaaggag
    13861 atgtcgtctg tatcattggc ccttcaggtt caggaaaaag tacattcttg cgttgcttga
    13921 atcgtttaga agaaccttct gacggtgaaa ttattattga cggcgcccac ttgatggata
    13981 aaagcacaga tatcaacttg gtgagacaac atatcggaat ggtattccaa cactttaacc
    14041 tattccctca tctttctatt ttggaaaaca tcattttagc accgatggac ctgaagaaag
    14101 aatctagaga agaagcagag aaaaaagcaa tcaaattact gcagacagtt ggtctagaag
    14161 ataaaaaaga tatgtatcct gaaatgcttt ctggtggaca aaaacagcgt gtggctattg
    14221 ctcgtgctct agcgatgaac cctgacatca tgctttttga tgagcctact tctgcactcg
    14281 atcctgaaat ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga
    14341 cgatggttat cgttacgcac gaaatggggt tcgctaaaga agtggccaat cgcgtcatgt
    14401 tcattgacga cggaaacttt ttagaagatg gaacgcctca acaaatcttt gaaaatccgc
    14461 aaaatgaacg gacaaaagat ttcttgaata aagtattaaa tatttaataa aaaccaaaaa
    14521 tgcacggtcg ccatgaaggg accgtgtatt tttatcttct tattttctca cctaatcgta
    14581 tcagccgcat cgcttgtttt cctgcacaaa caagcaatcg ctctacctgt tccatcgcat
    14641 aagaaagtac catcggttca ttgatgatat caagtacata gtcgatacca ccctcactta
    14701 attgagcttg atttttcttc tccatattat gtagttgtgg gacaactttc tgacaatcag
    14761 caattgtgtc aagaacggat caatccttta ttggagttgc tcgaaagggt cttctcttat
    14821 tacggacagg ctttgctcac tgtacataga caacaaatca ttatcctagt caatcggatt
    14881 tccagagcct cattgctatc attgcttgat aaaatccaga caaagtttaa taaaatgtat
    14941 cagttacagc taaactttgg aattgggtcg ctttgttata cagagcaaga gacaccgcaa
    15001 tctttccttc atgccaaaca agtttgcgaa tggatagctt tccatcaatc tgtgaacgag
    15061 atccgctttt ttgaagattt agacctcggg atcgttttac cagcaatccc gtcagatcaa
    15121 cgaacattat atgtaaaacg tattttgaaa agcctgacag aagaagaagt acatttgttc
    15181 aagaagactt tggcatgctt cagtaaaaat aacggaagca tcaaaaattg ctcagaagaa
    15241 ttatttattc ataaaaacac cttgcaatat cgattgaaca agttccattc tctcactggt
    15301 tataccccta gaaactatga cgattatcat attctaaagc tggctttttt gttggtacaa
    15361 acatgacaac tacaaaaaaa ccaggcgaac atatgattgt tcgtctggtt tttaattatt
    15421 aatgacttag cgtttttact agaaaaacta cttccgcttt ttcccgccaa aagcatcttt
    15481 aaacttatcg aagaaaccgt cttcttgctg ttcttcaact ttgatgccgc cagcttcagc
    15541 gaatgcacgt aatgcttctc tttgttgttc atttagattt ttcggtgtga tcagttttac
    15601 tttgacatgt tggtcgcctg ttgcaccacc acgtaacttc ggagcgcctt ttccacgtaa
    15661 acggaagttc gtacctgtct gtgttccagc agggatcttc aatttcacat ctccatggac
    15721 agttggtact tggacttcat cccccaaagc tgcttgtaca aaacttaaag gcaattcata
    15781 gtaaatttca gaaccttcac gatcaaagat atcactttct tctacacgga agaccacata
    15841 caaatcacca aatggtccgc cgttttctcc agcttctcct tgaccagcta aacgcatttg
    15901 ttgtccttct tctacaccag ctggtacatt cactttaact gaatgtgctt tcttctcatg
    15961 tcctgagcca tgacaggttg gacaaggttc tttgatttct ttccctgtcc catgacaaac
    16021 atcacaagtt tgacggctca tcactcgtcc aagtggtgtt tggcgttcta cattaatcgt
    16081 acctgcacca tgacatttat gacaagtagt tggttgtgtg cctggttttg caccgttccc
    16141 accacaagta tgacagattt cttcccgatt atacttgatt tctttttcta caccaaaaat
    16201 cgcttcttca aaagacaagt cgaccgtata ttgcaaatca gcaccttgac gaggcgcatt
    16261 tggatcgact gaccgaccgc ctcctccaaa gaaagagtcg aagatatctt caaaaccacc
    16321 gaagccgcca ccagagaagc cgccgaatcc accaaatccg ccaccagctc caccagcacc
    16381 gtaatttgga tctgtgcctg catgcccgta ttggtcatat gcagcgcgtt tttgcgggtc
    16441 acttaaaatc tcatatgctt ctgatacttc tttgaatttt tcttccgcat caggttcttt
    16501 attgatgtca ggatggtatt ttttcgaaag cttacgataa gctttcttta tttcatcttc
    16561 tgatgctcct tttgagagcc ctaagacttc ataataatca cgttttgttg ccataggctt
    16621 tcctcctatt ggttctcatc attttcacgt cgcttgttat catatcatat tttgtttgaa
    16681 acaaaaaccg atacttcaaa ctatttttca aaagaaaaag ccaaagtagc aactttggcc
    16741 ttctctcgaa attcagtgaa ctgattattt atcgtcgcca tcgacttctt caaaatcagc
    16801 atcaacgaca tcatctgctc cgccttgagc tgcttctggg ttttcttgag cttgttgttg
    16861 tgccgcttgt tcgtatagtt taacagtcaa gttttgaacg atttcgttca atgcatcgcg
    16921 tttagttttc atttcttcga tgttgtttgc ttcaacagcg gctttcaatt catcgcgagc
    16981 tgtttctgct tttttcactt cttcttcgtc cactttgcct tctaattctt tcaatgtttt
    17041 gtccacagag aatagcaatg catctacatc attacgtagg tcaacttcct ctttacgagc
    17101 tttatctgct tctgcatttg cttctgcatc ttttaccata cgttcgattt cttcgtctgt
    17161 tagacctgaa gaagatttga tcgtgatttt ttgttctttt tgtgtaccta gatctttcgc
    17221 agatacattt acaatcccgt ttttatcaat atcgaatgtt acttcgattt gtggaatacc
    17281 acgaggagct gctggaatat ctgtcaattg gaatcttcct agagttttgt tgtcagcagc
    17341 cattgggcgt tcaccttgta atacatggat gtctactgct ggttgattat ctgccgcagt
    17401 tgagaatact tgtgatttac ttgttggaat cgttgtattg cggtcaatca atttagtgaa
    17461 cactccaccc attgtttcga tacctaatga caatggtgta acgtcaagta aaacaacatc
    17521 tttcacatca ccagtgatca cgccaccttg gatagcagca cccatagcaa ctacttcatc
    17581 agggttaact gatttgtttg gttcttttcc tgtttcttta cgtactgctt ctacaactgc
    17641 agggatacga gtagatccac cgactaaaat aacttcgtcg atttctgatt gtgataaacc
    17701 tgcatctttt agcgcttgac gtactggtac ttttgtacgt tctactaagt cagctgttaa
    17761 ttcatcaaat tttgcacgtg tcaatgtcat ttccaaatgc aatggtccag attctcctgc
    17821 tgtgataaat ggcaagctga tttgtgtgct tgatacgcca gataaatctt ttttcgcttt
    17881 ttcagcagcg tctttcaagc gttgtaaagc cattttgtct tgagacaagt caatgctgtt
    17941 ttcttttttg aattctgcta ccatgtagtc gatgattttg ttatcaaagt cgtccccacc
    18001 tagatggttg tcgccagctg ttgacaatac atcaaagaca ccgtctccta gttcaaggat
    18061 agatacgtca aaagtaccgc caccaaggtc gaatactaag attttttcgt cgcgatctgt
    18121 tttatctaaa ccataggcaa gagcagctgc agttggttca ttgacgatac gttctacttc
    18181 tagaccagcg atcttaccag catcttttgt tgcttgacgt tgtgcatcat taaagtaagc
    18241 tggcacagtg ataactgctt tttcgacttt ttcacctaaa tagtcttctg cgaatccttt
    18301 gatatattga aggatcattg ctgagatttc ttgtggtgta tatgatttac cttctacatc
    18361 tactttgtaa cctgcttcac cgatatggcg tttgattgat gaaatcgtat ttgggtttgt
    18421 cacagcttga cgttttgcaa cttcccctac ttggatttct ccatttttga atgatacaac
    18481 agaaggtgtt gtacggtttc cttctgggtt agcgatgatt tttgcttcgt ttccttccaa
    18541 tactgctact gcagagttag tcgtacctaa gtcaataccg ataattttac tcataattaa
    18601 cgtctccttc tttaattgaa agtgtaagtt ttatatccgc acaattattg tgcaacaatg
    18661 accatggttg gtcttaacac gcgatcatgt aatttgtagc cttcttgtag tacttctaca
    18721 atcgtatcgg ctggtgtatc ttcagtcgct ggaacagttt gtactgcttg atgaagatta
    18781 ggatcaaagg cttctccttt agcagggatt ttttcaattc cttcttcttc cagtgcgtta
    18841 cgcaaacttt ccaagaccat ttccacgcct tttttcaaac ctgccccttg atcatctgac
    18901 acttctgtgg ctaatgcacg ttccaggttg tcaattgatg gaagtaattt tttggctaaa
    18961 tcttgtgaac ggtatttttg taattgctca cgctcatttt ttcctcgatt tgccatattt
    19021 gcgatttcag cacgagcccg caagtattta tcttccattt cttctaactc agcttttaat
    19081 ttcgctgttt cgaattcttc cgcttcagct tctgaaatac ccgaatcttc tgcagcttcg
    19141 acatcgattt ccggttctgg ctgagcatct gtcatttctt tatctagttc ttcttgattt
    19201 tctttcacga tacagtcttc ctttcattat tcagtcagct gtttgattgc tcttactaag
    19261 aaagagagaa cgatcagctg caaatagctt cgttattcac attacgagtt tgacaagtca
    19321 agcgaacggt agtaatcagc aagtttagct gctagttctc ttcgataaac atcgactaga
    19381 ccgaacatcc gagaataagg catactagta ggccctaaca aggctattgt acctcttcca
    19441 tgaccatcta tctcgtaact tgcttgaatc atactcatat tctgcaataa atcattgcct
    19501 aattcagcac caatcttgat atgaatattg ctatccatcg gagtgatcat ttgtgtaagt
    19561 ccgtctgagt ctttcataaa agaaaacatc gatttgaact gatcaagatc ttgacggggc
    19621 tcataatcca agagattcat ttgtccactg acataaacct tatcttcaaa aacatgacca
    19681 agaacggtgt caaataactg agacatccct tctgtcgttt ggaaatattt atgaagaatc
    19741 atcggaatct ccgtacgaag tttatggtac accgtcatca gaggatctcc gactagacgg
    19801 tcgtttacaa tctgtaccat tttttctagt tcctgacttc ccatatattc gggaagagtg
    19861 aacacttggc tttctacatt ccccatatct gttacgacga tagcaataat ctgtcgatta
    19921 ttcaagggta cgatacgaaa gccagtcagt cggcggtctt ttatttcagg tcccaatgaa
    19981 aaagtcgtat agcttgtcag ctcagataat atctcagcag attgcttaat aatctcattg
    20041 atttcatgaa attctctgcc tagagactga cgaatcagca gcaaatcatc ttctgctacg
    20101 cgagcaggtt ttaacaaatg atccacgtaa taacgatagc ctaaagcaga tggaatccgc
    20161 ccagaagaag agtgagtctt caacagcaat ccttcatctt ctaatgcttt catatcattg
    20221 cgaatcgttg ctgagctggc cttgactccc tcagacatca atgttttgga accaacgggc
    20281 acgccactac tcgtatagtg ttggatgatc agacgtaaaa tgtcgttttg tcgctttgtt
    20341 aacatcttcc tcacctctca ttagcactca tatattctaa gtgctaatcc acttataaat
    20401 atagcaagaa gcccttctcc tgtcaagaga aagactccat tttttagcac tctaaacaca
    20461 agagtgctaa ttctaaataa ttgatgtaaa tccagttata tcaacgtttc aatagtcctt
    20521 tattgtattt ttgaactttt tttatttaaa ttcagataaa aaacaaatat tctttaaact
    20581 atttttcaaa caaaaagcgc tcaaacacat tgttccccaa aaaggtccct cttggtgtca
    20641 aataaacacg gtcttcttca ttaacaagta acccttcttg ctctaattcc cttagcacat
    20701 caccgtacac cgattcaatt gttttttgat agcgatcata gaaatgtttt ttagtaatcc
    20761 ctgttttttt tcttagtcct aagaacattt cttcttctat ttgatttttt cgtgtcaatt
    20821 tttcttcttc caataccggg cgttcatttg ctcgtaatgg gcgtaaatag tgttggattg
    20881 gtcctttgtt gcggtaacga atatctccaa gataaccgct agcaccagct ccaaacccaa
    20941 aatagtggtc attgttccag tacatgaggt tatgttgaga ttcatgtccg gagagcccaa
    21001 agttacttat ttcatattgg tgacgtccat gcttttccat tgcttcaatc gtctcttcaa
    21061 acattctggt ttccgtttcc tgatcaggca gttcgagtct tccttgtcgt acccagttca
    21121 tgaacattgt tttgttttcg aggatcaatg agtacaaaga atagtgaggc agatccaaag
    21181 ccaaagcttt ttccaaagta tccagatatc cttctagtgt ttgtcccggt aatgcataaa
    21241 tcaaatcgat gcttacattg gtaaaatttt ctgcttctaa aaattgcata gtttgataca
    21301 catcttctgc ggtgtgttta cgtccaattt ttttcaataa gcgattatca aatgtctgca
    21361 ctcccatgga taaacgattg acgccatagt ttttcattac ttgaagtttt tcacgggtca
    21421 aatcacctgg attcgcttct acagtaaact cattaccatt ttgaaaaggc aggatctctc
    21481 tagcacctga taacagtcga tcaagttgtt tagcagataa agaagttggt gtcccgcccc
    21541 ctacgtaaag tgtttctaaa ttcgctgtcg gatgactttc tagcattatt ttcatttcat
    21601 tcagcagcat ttccacatac tcatctactg gctgcccttc taaaaaaact ttattaaaat
    21661 cacagtaata gcagatatgc tcgcaaaatg ggatatgaat gtacgcagaa accttgtttt
    21721 ctgcgggatt tattgtttct atcattgaaa ttctttctcc ttacgtcgaa aagtcgtgcc
    21781 tgttttcgac cttcttaaaa tcgcgttcaa gcaccaactc attgaaataa gccgcatatt
    21841 tagaatcaag gaaacctagc tctaaaaatg cttgcttatt gtcatcggag ctacacggtg
    21901 cttttacagc ctctcgataa acggtaatcg caaagctgac cttcggcatt aagtcaaaat
    21961 ctgcaaaaat atgaaaagct attttcacag attcctcctt aagtgctcag accacaacgc
    22021 tttgcttaca acctctcata tacgcatact aaacgtattt ggcgaacaaa tccactaaaa
    22081 aatccaagaa tcgacttctt ggatgatttt ttctttcatt aaactggtta cctagttata
    22141 tatctctagg gttgttttca tgtctgagtg cccgatatga tttatttttc gtttctataa
    22201 ttactttttg ccttccagtt tcttttaata ttgtactcaa gcaagtggga attggttctt
    22261 ttgtcatcca tgtatgcaaa atgttataat gctggtaaac aaaaatgatt acctgtcatt
    22321 tttatttagt tcgtatttaa ttaagttgac tatctgtata ctctgtctaa aacttaagaa
    22381 ttgttgtttt cgctgtcgct acccaatttt aatgaatgtc tgtaggttaa aactgttgtc
    22441 cttgttatag atagtaaagg agagcttgaa aatgaaaact gcgattacgt ttctattaga
    22501 ggaatatgcc gattgggaag gtgcttatat tactagcaca ctaaaccaaa atgagaaatg
    22561 gcaaaacaaa actgcttcta tccaatcaga ggtcacttcc attggaggat taaaaacaaa
    22621 aatcgattac ttaatcgaac aaatacctga aaaagttgac ctttttatta tgatcggtgg
    22681 caattcatgg gacataaaaa atcatcattt atatcaaata atcgataaat tgttaaaaac
    22741 agacgtacca gtagctgcta tttgtggggc agttgattat ttagctcaga gcggattatt
    22801 gcaaggttac aaacacaccg gaaatgcgca atttccttgg ttagaatcgg aaaattatca
    22861 gaataaagaa gattttatag aacaacaagc tgtgaaagat cgtaatttga ttactgcgaa
    22921 tggaacagct cctatagaat tcacagactt gattcttcaa gcagtccatt ttgaaacaga
    22981 agaaataaga gaaaaaacga ttcgtctata caaacttgga ttctatgatt actataaaaa
    23041 gtacggagat cctttctcct aatcattctc ttcaattaaa tactccttat caatagtgtg
    23101 acaagtaatt ttactgatga ttatttgtca cactttttct gacatttatc aaagaagata
    23161 cactctattt atattcatag acaaggtttt acggattcaa aaattcagga ttatgctata
    23221 ctggtattaa aaaaataatg aggtatttta tatgaaaaag ttaggtttta ttttattgtt
    23281 ggtctcttta ttccttgtag gatgtacgtc tcctagtaaa gaaaatagtg atccatctgc
    23341 gaccacagac aaaagttcaa tcagacaaac cagttcagct gaaaaatcaa gtactcagaa
    23401 atcagcttct actgaaacga atacatcttc aacaagcagt aaagtctcag agcccaatac
    23461 ttcttctaca tctagtagta cagttgagac aaatatttct tcttcaacaa tagacacaac
    23521 ctcctcacag tcttctcagt caatagatcc gttagcagga tattctgatc tacaaattga
    23581 gtacgcacgt gtttggctag ctattagagg taatactttt agagatacat cgggtgattt
    23641 cgaattacat gttattcatt atcctgcagg aacacctatc gccccttatg atgcagggag
    23701 tgcagtctat ccgaaagatg tggttatgct cactgggaaa tacagttacc agggagtttt
    23761 agtgtattca agtaaccatg acgggacaat cactagttat ccagttcctt cacattggca
    23821 aatgcctgca gatcaaacat cttatccagc gttcattaga gaaacaactc aagaaatcat
    23881 tgataatgca tctgtagtag ctattcctac aggaaatccc aatgacgtga aacagttaat
    23941 cgatgtgaca gttatagata attagacttt ctaatatgtt atacaaaaag aagctgacag
    24001 cattttgtca gcttctttca ctttcttttc tatttagatt tattggtctt tgatagactc
    24061 caaggcagga aaaaattttt taaataaacg tttgcaaaac agtcgctttt ctatcttcta
    24121 ccgctacttc tgcataggca ccattaatca atgtcagttg tggcggtaca tgaccacaat
    24181 caatatcata caagatcggt agttttaatt cctttgacag atcttcgtat acatccttca
    24241 ttttataatc taacaaatcc tctgccgcac ttctaccgaa catgatcccc gagctgccat
    24301 caaaccagcc tgccagtttc atctgaacaa gtgatcttct taaatcaact gacgatagct
    24361 cacagttttc tagaaaccac aagatcggct ttccatcatt atactttctt ttaaaagatt
    24421 ggacatctcc ataaggagta ccgattaaat gcctgatcgt atcaatacaa ccacctaata
    24481 atcgtccctt catccttact gcatctccgc tgactgattg ccaatttgtt ggttcagtga
    24541 gatggaaaac agaagatgat gaatgctcat ggggccacac tttttgatac ttttcagaag
    24601 attgctgtag aatcgattct ccttctcttg ttttcagtac actttcccac attgctgtcg
    24661 tttcatccaa gtactctcct cttaaatcaa taaggttagt tccatgtgca gtggctatcc
    24721 ctgtttttaa tgtgacagca agtagcaacg tactaatatc tgaataacct actatccatt
    24781 ttggttgtag agcatcaaaa tcgacaaaat cgagaatctc aatcaataat tctcctcccc
    24841 aagtcggaaa gatcatttgg atattttcat cttgcatcat ttgattgaat tcatctgctc
    24901 gttttcttgc tggacctgat ttttccttat cttgcgtcca aagcgtcttt ccagtaacta
    24961 cttggaaaga tttttcttcc attctcttta aagctgattc gagtaacgaa tggtgttcct
    25021 ctgatacgcc tgaggatggt gcagtaaccc caatcgttgc atttctttct aataatggat
    25081 aacttatcat gttgcttcac ctccaagtat gcactatttt acttggttaa ttgttttttt
    25141 cgtttgccgg catcaaaaat atcggcatct caagtagctg gatgattttg tttaagtcaa
    25201 ccgtttggat gaccatcgtt tgatctggtc tacctggtgc aaaaacaagt ttttcatttg
    25261 ataagagctc ttcatcaata ataatcggaa tctgtttttc ataaccaaac ggagaaacag
    25321 cgccctgctt ttgtcccgtt tgttcttcca tgcgttctga tgaaacaata gaaagccttt
    25381 taccgacgat cttccctaat gctttgaaat ccgtttgttt cgtagtaaaa gtcagatata
    25441 cgtagttatt atctttttta tcttttaaga ataagctttt ggtctctgta cctgtaaatc
    25501 cttgttcttt ctttattacc aaagcatctt catttgtata aatggctcga tgatgataca
    25561 actcaaaata tatattttcg tgtaagaatt tctttgccaa gtcaatcatt tttatgtctc
    25621 ttttctattt tattatttga gacatcttat catataaata tagcggttac aacattactt
    25681 gtgttttgtt cgttttttat acataactat tgtttttctg gtaaaataaa tcaaaccact
    25741 taatggaggc gttttagaat ggaaaaattt cagccgatca tcaatcaaat caaagaaaaa
    25801 gaacatcaac aaaagatcaa agaccttttt gctaagataa cagaaacgta tccccagcta
    25861 gacacaactg taaaatggaa ccaacctatg tttactgacc atggtacctt tattattgca
    25921 tttagttttg ctaaaaatca tttttctatc gcaccggaaa aagcagcgat tcgtgccctt
    25981 gaaaaaaata tccaagaagc aggctacgta tatacggata acgtcatcaa agttccttgg
    26041 aaaagtgtaa tcaactggga attgattgaa caattgattt catttaatat tgaagataaa
    26101 aaaggtcatg ataaattttg gcgataaagc tttatacgaa taaacctttt actggcgagg
    26161 aaactattca tcaccttata caggttttgt tcaatcctat cctttttatc atccctggtt
    26221 aaacacttaa ttgacaaagt atctgctatt tttataagga gtctttcttg tgctgaaaaa
    26281 aatcactgat tgaatggctt tgaattttct acatagtaaa attatttttt cagcaatttt
    26341 cctgcaaaac ttttgaataa ggaaaaaata cgagaaaata tcagtgatct ttcataatac
    26401 tgcccattat tttcaggtat atttaaatat aatatcactt taggagagaa cgaatttctt
    26461 tagagaaata aaagttagaa ttttctgact tgatttttac aatgacctta attaaaatcg
    26521 ttctttctac atttcgtgat taagcaaata tcaactaagt cagtagaacc attcacatag
    26581 gagggtgaac attgaaagat ttttactcag taaatgaatt agccgaacaa ttaggtgtta
    26641 ctacacgttc tatcagaaat tatcttcatg aaggaaaact aaaaggaaca aaggttggcg
    26701 ggcaatggaa attttctgaa cgtaatcttt ttgagttcct atacggagat caagcagagg
    26761 aagcagcaaa agaaatgcag cgattcatgc taaatgcacc aatcacgatg cgtttcaacc
    26821 ttcaatatcg tgattttaca gcaatcaatc aattccgaga acaattagtc cagtaccata
    26881 acgatgttta cgcaaataaa aaagaccgtc ttttacaata cgatctttat aaagacaatc
    26941 atgcggagat actaatcggt ggtaacttca attatgtcgt aaactttagt caatggatca
    27001 atgaaaaatt attgatccag acagatattt cccttgtatc ttaaacaatt aaaaagcgct
    27061 agaaattcca gagatgttgg aaattctagc gctatttttt tataagtact tactgtttgg
    27121 ttcaaacaaa tattttttta ataaatgaaa ttttaccatc tttatcctca aataaagacc
    27181 gtgaccatgc ttcctactac gatcagcaat aatccgataa tcgttaattt tttttctttt
    27241 ggtgtcttat tttcatgcag gatgtacata ccgcctaatg tagagataac tacgtttagc
    27301 tgtgtaatga tatatgcagt agcaacccca ttttcatttg ctgaaaaaat ataagccaat
    27361 gctgacacac taaagatggc tccaacgatc gttgctttcc aactcttgtc ttcagaaaat
    27421 gcggttggtt tacggaaaat cacataaatc actgccccta aaaagacacc gatcatttgc
    27481 ggaaagaaaa tggaaagtcc gcttgcatca actagtttag gcaatgcact gtatacccaa
    27541 taaccaatac ttgttgatgc taatagacta attccttttg tgataccgcc tttttttcca
    27601 ggatcatcac tgattgccgt taaagcagaa ccaaatacaa gaagaatgat tgctgctgct
    27661 ccaatgattt tccccatact tccagaccat tctccaaaag cgaagacacc aatcaacgag
    27721 gttccgacta actgtaatgc agtcgaaatt ggcatcgttt tagaaacacc aatcaagttg
    27781 aaagatgtat actgtcctgt ttgtccaata acccaaaatg caccagaaag taaactaatc
    27841 aaaaatgtag tcactgaaat accagctgga gaagcgaata attgaacgag taagccgaca
    27901 agcaccgctc caataccagt tcctaatatc tcatttgttg gtctgccacc aatctttttc
    27961 aaaatcagtg gctggatccc ccagccaact gccgggatca atgcaatcaa taaacccatt
    28021 tttattcacc cttatccttt tccaccttgg aaagaaggat ataaagtcat cccaccatct
    28081 gcaaatagtg taatacctgt aacataactg gattcgtctg aagccaacca tgcagctgtc
    28141 gccgctattt cttttggttc acctacgcgt cctagtggga tcatactttc caagagttct
    28201 ttttgttcag gatcatcaaa ttttgccgca ttaataggtg tacggatagc accagggcct
    28261 atactattga ttctgattcc cttcggtgca tactccattg ctaccgtttc ggtaaataat
    28321 tttaccccgc cttttgaagc agcgtaatga gcaaacgttg gccatgggat ctgttcatga
    28381 acagaagaca tattgatgat atttcctttt ttattattct ctgtaaagta atttaaagcc
    28441 gctttcgttc cgataaaaac gccagtcaaa ttgactttta acactctttc ccaatcttca
    28501 aggctaagtt catgggttgg atgctgattt tccatcccag cattattaac ccaaacgtcc
    28561 agtgtcccaa aattttctaa agccgtatca agtaaacgct gtattccttc ttcagaactg
    28621 acatctgctt gtacagagac tgcttttccg cctgcctctt cgattgcttt gactgctttt
    28681 tctgctcctt ctttatccga gttatagttg acaactacat tcattttttc tttcccgaat
    28741 cgttgagcaa tcgctgttcc aatccccttt gagccaccag ttactactgc gactttgttt
    28801 tctaattcct tgtacatagt acccactcct ttttcatacc taaacctgtt attacggata
    28861 acttttattt ccgccttaaa gtgagtgtac accaagatat atagtctaaa gcaaatgata
    28921 cgctctagca atcgttttca tgaattgcta gtgtcctttt atttctataa agaaagaggt
    28981 tgtgacagaa gtggtcagct tcaagaaatt tcggcttatt tccgaagaag ctacttctga
    29041 aaaaccattt atttggaaaa aaaggctgtg acaagacttt tgtcacagcc cctttcctgg
    29101 gcttatttat cacccatttt attgtaattg ctagttatac ataaacaaga gattttatgc
    29161 ataatgtttt attgctgaga aaaatattca gctgttgctt gttcatcctc cttgagttgt
    29221 tcaatcaatt cctctacact agaaaatttc acttgatctc ttaaaaattg attccagcgt
    29281 accgcgacat gttcaccata gatatcttga ttaaagtcca aaatatacaa ctctacagtc
    29341 agctcccgtc cttcaccgaa tgtatcatta tgtccaattg aacccatggc tggataccat
    29401 gtttccccaa tttttatttc cgacacataa acaccttctt taggcaaatg aaccgtgctc
    29461 tttactttga catttgctgt agggaatcct agtagtctgc ctcgtgcatc gccatggaca
    29521 actacccctt ctacctcata tgtgcaacct aatagttttg ctgcttcttt tacttgtccg
    29581 ctgtctagta atccacgaat acgggtagag ctgatttttt caccttgttc ttcttcttta
    29641 gaaactgtga cgattttgaa tcttcctttg gcatattcag gaaaatgagc gacatctgca
    29701 atgtcttttg gtccatacgt ataatcaaaa ccggaaacag catattttgc atgtaaccct
    29761 acgatgtatt gatcaacaaa ttcctgcggt gctaaatgag caaaagaaga agtaaaatca
    29821 acttcatata aataatctat acccaaagcg gccatttttt cttctttttg cttaaggcta
    29881 gtcaaatatt tcacgctgct aggatcgatc tttttaaaga caatagatgg atgctgattg
    29941 aaagtcatca atgctaattt caatccttct ttttgtgcaa tttccatacc tgtctttatg
    30001 actttctggt gtccgatatg cacgccgtcg aaaaatccca gcactaaaac gacatcgtct
    30061 tctggaattt gttctgattg gtaaggatgt ctgatcttga tgatattcat tcgttattcc
    30121 tcatttctca atactttact aggttttaat ttgttttttt ctttcgggtt tggctgataa
    30181 atactgacaa cttttccttt ataaaacaaa gcaatttctt ctgacggcat ttctgggagc
    30241 gcaaatacag tataatcaag tctcataccg ttttttactt tctgccatac ttcatcagta
    30301 atatctactc gtttgaaccg ttccactcct ttttctattg gcagcaaaca atcatcgatt
    30361 gtcccatttt tcacagcttg agctacttct tctaatgtca gagcatcctt ctgggacata
    30421 ccggcacttg ctgttcgggt cagatcagac atgtgcgcag ggtaaccaag tttcttgcct
    30481 gtatctacag ctaatgtgcg gacataggtt cctttcccgc attctacttc gaagcgccaa
    30541 gaaatggttc ctgtcacttc gtctcttgtc aattcacttg tccggataaa acggtaaatc
    30601 tgagcctttc taacaggacg ctccactgtt tcattattgc gggcatattc atataaccgc
    30661 ttaccgttga cttttaccgc tgagtacatt ggtggtatct gggtgatctc accaataaat
    30721 gaagccatca cttcatctat ttttttttca gataaagaca tatctactgg ttttgtttct
    30781 acccgttctc cggatcgatc ttccgtcgtt gtactgaaac ctagagttat ctcgcctaca
    30841 tagattttcc cagaatctgt caaatattcg ataacttttg tggcttttcc aatacaaatg
    30901 ggcaacacac cgtctacgtc tgggtcaagc gtacccccat gtccgatttt tttcgtatgt
    30961 aagatttttc ttaatttgaa tacgcaatca tgacttgtca ttccgcgttc tttccataaa
    31021 ggtaataacc cgtccatgtc ttttctccat ttcatttttg atttaactga attattataa
    31081 cacattgcat caattactga cgaatttgac atcataatga acggttttta tttttcggca
    31141 ataaaaacgt aacaaaattc atgctcacgt aaatagaaat catgttatgc ttttttaaag
    31201 gagggaatag aatggaagat tttttctttg caccatttga ttggatttat gggtggctta
    31261 caggtaaata gtttggtttt cacttcccaa aaacgaaaaa aaagactggc cattcttcca
    31321 gtcttttttc cgtttttttt atttattttt tcagtttctt aatcaatgta aaaaattcta
    31381 ttttgcggga tcgattttat cattttattg gtcaagattt ttccatttcc aatccaatac
    31441 ttctggcaga tcaataccat tttcacgaat atagttttga tgttcttgta atttctgatc
    31501 catttttttc gcaaaatctt ctgcttgact tcctttgatt tttaaagctg cgtcttttgc
    31561 caaatgataa cgatcaagtt catttactac acgcatgtca aatggtgtcg tgatatctcc
    31621 attttctttg tagccatgaa tcattaaatg atggttatga cgatcaaaga aaatcgtgcg
    31681 gaccaatgtt tcataaccgt ggaaagcaaa taagatcggt ttgtcttttg taaaatattg
    31741 atcaaattct tcatcgctca acccacgcgg atcttgactt ggatgacgta atttcaataa
    31801 atctacgaca ttgatgaacc gaatttttag ttttgggaat tcttccagca gcaaagttac
    31861 agctgctaat gcttctaagt tagattccgt tcctgcagca gcaatgacca attcaggttc
    31921 ctcctctttt gctgtcgaag cccaatcaat gattttcaag ccttcattca ctaagacggc
    31981 tgcttcttct gctgagtaaa attgagcacg cggatgtttg gaagagatga tcaaattgat
    32041 tttttctgac gagcggaatg ctttatccat gactgcgagc aatgtatttg catccgcagg
    32101 cagatattca cggatgaact ccgccttctt ttctgccaaa tgtgtcaata ttcctggatc
    32161 ttggtggcta tatccattgt gatcttgctg gaagactgtt gagcttgcaa tgagatttag
    32221 cgaaggataa tcatttcgcc aagaaaggtc atgtgatttc cgcatccatt tgaagtgctg
    32281 agtcaacata gagtcaacca ctcgcaagaa tgattcgtaa cttgcaaaaa accatgccgt
    32341 ccagtcagaa cgtacccttc taagaaccct tcatcttggt gttccgataa ttgactgtcg
    32401 atgacgcgac cagatggcga aagccattca tcattttctg gttcgatttt ttccatccat
    32461 tggcgatttg tcgttttgaa aacttgattc aatcggtttg attttgtttc atctggtcca
    32521 aatatacgga aattttctgg atttttctca atgatctcgc gaacgaattt accaagttcg
    32581 gtcatatctt ctgctttaac ggagcctggt ttttcaaatt gcaaagtata ttttttccaa
    32641 tctggcataa taagcggtct tggatcgatt ccaccattgg tgataggatt ttttgccatt
    32701 ctttggtcac cagtaggcgc gatttctctg atttcttcta aaatacgtcc ttgtgcatca
    32761 aataattttt ctggttcata ggatttcagc caacggagta aagcatctgc atgatccata
    32821 tgttcttgat ctacaggaat cggtacttgg tgtgctcgga aggttccttc aattggttcc
    32881 ccgtcccatt cttttggacc ggtccatcct tttggtgtgc gtacgatcaa tactggccat
    32941 tttggcatca cgacatctgt tgctgtcttc aggcgagctt ctttttggat ggatttaatt
    33001 ttctcgattg cttgatccat tttttcagcc ataacaggat ttaatttttc tgaatcattt
    33061 ccttcaataa agaaaggttc ccagccaagt ccgttaaagt aatttgctaa ttctccatct
    33121 gatttacgtg ccaagatcgt tgggttggct atttttgcac catttaaatg aaggatcggt
    33181 aaaacagctc catctatcac tggattgata aagacatttg agaaccagct tccagctaaa
    33241 ggaccggttt ctgcttcacc atcaccaatc acgacagcag agattacttc aggattgtct
    33301 aatacagctc cgactgcatg tgaaagagag tatccaagct ctccgccttc atggatagaa
    33361 cctggtgttt ctggtgctgc atgagaagca atgcctccag gaaatgaaaa tcgtttgaat
    33421 aatttttgca ttcctgtctc atcttccgtt acttctggat agatctctgt gtaacttcca
    33481 tctaaataag cattcgatac catgacttgt ccgccgtgtc ctggcccttc aatataaaac
    33541 atgttcaaat catatttatt gatgactcgg ttcaaatgga cataaataaa attttggcca
    33601 ggaatcgttc cccaatgacc gattggatgt tttttgacat cttcgggttt cagtgtacgt
    33661 ctcagtaagg ggttatcttt aagataaagt tgcccaacag aaagataatt agcagcacgc
    33721 caccaagccg tcattttatc aaagtattct ttactagaat aatccataca taacactcct
    33781 tttcattatt actcttattc tagcgcagat actcccaatt gaaacctaat aagcacttct
    33841 gttcatttgt ttggatttta tatctagcca aatgaacagg tataaaaaac cacgaaattt
    33901 ttcctttcgc tgacgttttt tacattgatc caaaaaactt gtcgttatca caatggctgt
    33961 tccgcagttc atttcgctcc tcatcatgca taatttgctg aatgacgctg ggccagttaa
    34021 cacattgctg gaaaactggg gctggatcga gcaattcctt tcctgattga tccgttttat
    34081 gtaaaagtct ctgttatctt tgtcaatatg tgaattggta tcccagctac tatgctaatt
    34141 tctacaggga ttatccaaaa tttggatcgt gaccaaatcg aagcggcgta atcggaaata
    34201 tcaataactt caacgtgatt tttcttttaa ctgtcaacgg accgacaaac tcaaactttt
    34261 atggtgcagg ttccactgat cttctagtta cttggctata cctgactgtc actaaaatag
    34321 attacaatct agcttccgtt atcggtatct tgatctttat ctatatgcaa aaatttatat
    34381 actactagaa aaaagaaagt cgaatttttt acttttcttt ttctcttttt tggttagctg
    34441 acacgccgca agactaaagt catggaactc ttaggtaata cgtcttaatg aaaacaaaaa
    34501 aatcccgcaa cccctgattt cacaggattt gcgggagttt ttgattagtc tttgttcaag
    34561 tcgcgaagca attcatcgat atggttgcca tagacaactg attcatcaag ttcaaagaac
    34621 aattcaggtg tcttataaat acttagattg tgccctaact ctcgacgaat caatcctgat
    34681 gctttattca atccatcttg tgctgttttc ttatctgatg ctaagtcaga taaaatgctg
    34741 taatagatcg ttgcttgctg taagtccccc gtaactttca catctgtgat cgttacgttc
    34801 tccacacgag ggtcacggac ctttttgcgc aaaatatcat tgacttcttt taagatttct
    34861 tgaccgactc ggcggtcacg gtagttagcc ataatgattg gcctcctttt atcagttttt
    34921 agtcattttt gacttcttcc atgataaatc cttcaattac gtcatctact ttgatgtcat
    34981 tgaatttttc aatcattgca ccacattcat aacccatttt gacttctttc acgtcatctt
    35041 tgaatcgttt cagacttgca agttggcctt catagattac gattccgtca cggattacac
    35101 ggacaccgct atctctacga atatatcctt ctgttacata ggcacctgca atcgtaccaa
    35161 ctttagaaac tttgaaggtt tcacgaacga tcatttgacc ggtgattttt tcttcaaatt
    35221 cagggtctag catccctttc atcgcagttt cgatttcttc gatggcttta tagatgatac
    35281 ggtgaagacg aatatcgact tcttcagtat cagcttgcac ttttgcctgc ggtgtaggac
    35341 gaacgttgaa accgatgata atagcgttgc tggctgctgc taaggttaca tcactttcat
    35401 tgattgctcc gacagctgaa tggacgattt ttacacggac accttccaca tcaatcttct
    35461 tcaatgatgc agccagtgct tctgctgaac cttgtacatc tgctttgatg attacgttaa
    35521 cttctttcaa ttcgccttct ttcaggcttt caaataagtt atccaaagtc acacggcttg
    35581 tggcagcacg gttttctaat aatgcacgtt tcgcgcgttc ttcacccgct gcacgtgctg
    35641 tcttttcgtc ttcgaagact acgaaacggt cacctgcttg aggtacatca ttcaagccag
    35701 tgatttctac tggcgttgca ggtcctgctt ctttatcgcg acggccaaga tcgtttgtca
    35761 ttacacggac acgtccgtaa gtattaccga caacgattgg gtcaccaaca tgaagtgttc
    35821 cttcttggac aagtaaggtt gacacaggac ctttaccttt atcaagacgt gcttcgatca
    35881 ctgttccgat tgcgcgttgt gttggatctg cttttaaatc ttctacttct gcgactaaca
    35941 ggatcatttc cagcaattct tcgatgtttt ggccgaattt cgctgagatc tcaacaaaga
    36001 tcgtttctcc accccatgct tcaggaatta actcatactc acttaattct tgcataacat
    36061 gttgagggtt tgcacctggt ttatcaattt tatttacagc gacgatgatt ggaacgcctg
    36121 cagctttggc atggttgatt gcttcaactg tttgtggcat gacgccatca tctgcagcaa
    36181 caactaagat cgtgatatct gtgatacttg ctccacgtgc acgcatactt gtaaaggccg
    36241 catgtcctgg tgtatccaag aaagtgattg gtttaccatc aatatcgatc tggtaggcac
    36301 cgatatgctg tgtgattccg ccggcttcgc cgcttgttac acgtgaatgg cgtaatgtat
    36361 ccaataaagt tgttttccca tggtctacgt gtcccataat cgttacgact ggcggacgtg
    36421 agactaattt atctggatcg acttcttctg cctcgaagaa tttatcgata tcagcaatgt
    36481 caacttggac tttttcttgt ggttccatgc catagtccgt cgctaacagt tcaatcgtat
    36541 ctttatccag tgcttggttt tgattgacca tgacaccaag cataaataat tttttaatga
    36601 tttctgctgg ctcacgatga atttttttcg caatatctgc tacgttcata ccttcagtat
    36661 actctaatac atctggcaat tcacggaatt tgcgtggtgg tacagctggt ttagttgatt
    36721 gttgctgttt gcctttttta ccttttttat tataacgatt acggttatta ttattaaatt
    36781 tgccgcgatt gttattgttt ggtttgttgt tattgttagt acggttttga ttgccgcctt
    36841 ggctcgttcc ttgacgattt tggctgctgg tatttcccgt attattttgg tttgttgttg
    36901 gtttttttcc ttgattcact tgaccgctcc cttgaccgcg atcttgataa ctgcggttat
    36961 ttttattttt ttgttgattg ttcgttgttt cttttttacg ttgatcgttc ggctgttgat
    37021 tggctggttt tctgcttgct tgttgtgcag cttgttgttt cgtgcttggg tttttgaacg
    37081 cttgttgcaa tttcttctca tcattgctag taatcgcacc catatggttt ttcacatcga
    37141 tgccaagttg ttgtgctttt tccacgacgt ctttactaga ttttttgagt tcttttgcta
    37201 attcatagat tcttttgttg cccatgcaat caccttccta accttcaatt agctccttgg
    37261 tcttcttcgc aaatccggca tccattattc cgaatacctt gcgaggtttt ccgatcatct
    37321 gcgtgatttc tgcttcagaa aatagctcaa agcaaggaac ttcataatat gaactcttat
    37381 cttttatctt ttttcttgta ttctcgcttg catcggttgc cacaaacacc aattttgctt
    37441 tttgtttgcg gatttcagca atggtcagtt cttcacccgt gattagtttt cccgcgcgca
    37501 tcgctagacc gatgagattc atggcttttt gccgattaac tgtattcatt ttccaaataa
    37561 ctctttacgt gctttttggt gagtgacata gtcaagaagt tcttgataaa actcatctgt
    37621 taatgttgtt tcgagtactc gatcgagaat ttttttatcc caagcttgtt gtacttcttc
    37681 cggctcaagt gagacatacg ctcctcgtcc aggcattttt ccagttggat cgatcgatac
    37741 ttccccttct tttgaacggg tgattcggac catctctttt ttaggtttca tttcacctga
    37801 aaccacggat ttgcgtaaag gaattttcct ttgtttcatc tctttgcctc cttactaaag
    37861 ttaagcttct ggtttttctt cgaccgtttc attgttgaat gcaattgttt catattcatc
    37921 gtctgttaaa tctgactgaa cgattgcttc atcatgcagt tcttctgttt ctttttgttg
    37981 ttcttgattt tcataaaatt ccgtcatgtc agattctgat ttgatatcga ttttatggtt
    38041 agtcaattta gcagctaaac gtgcgttttg tccgcgttta ccgattgcta gcgataattg
    38101 gtagtctgga acgacaactg tgcaggcctt tggatttgtc tcatcgaaga tcacatccat
    38161 gacttgtgca ggattcaatg catttgcaat gaatacagct ggatcttcat tccattcgac
    38221 gatatccatg ttttcgcctt ttaattcatt gacgattgct tgaactcgtt gtccctttgg
    38281 cccgacacat gtaccgactg catcgatatt tggatcattt gagcgaactg ccacttttga
    38341 acgatcgcca gcttcacggg cgatgctgac aatttcaacg attccatcat agacttctgg
    38401 tacttcttgc tcaaataagc ggcgcaatag gtctggatgg ctgcggctaa caaatacttg
    38461 cggaccttta gacgtatttt ctactcggga aacataaact ttgattcgat catgtggttg
    38521 ataaaattca tttggcattt gatcttgttt agaaagaact gcctcaattt taccaagatt
    38581 gacataaata taacgtttat cctggcgttc gacgattcct tgcatgatgt ctttttcata
    38641 agcactgaat tcgttataaa taatcgttct ttctgcttca cgtacacgct gtaaaataac
    38701 ttgtttagcc gtttgcgcag cgatccgccc aaaatctttt ggtgttactt caaaacggat
    38761 cgtatcaccg atctcatatg ctggattgat taaaagtgca tctttcaatg agacttccaa
    38821 ctgtgaatcc atcacttctt ccgttacttc ttttaccgca tagacatgga tctttccttt
    38881 tttctgatca aattccactt cgacgttttg tgcttggcca tagtgacgtt tgtatgcaga
    38941 aaccaatgct gcttctaaag catcgatcac gatttctttt gagatccctt tttcagcttc
    39001 taaagcgtct aacgcattta acatttcttt actcattttt tctgattcct ctccgtgatt
    39061 aaaattggat agctaagcga gcttttgcaa tattttttcg gtcaaaagtc aattcttttt
    39121 ctcgtgtttt tatgcgtatt ttcaatgtaa gctgttcagc atcaaatgat tgaagaaatc
    39181 cttcatattg tttttcgcca tctactggtt ggtataagga gacatggaca taatcaccaa
    39241 gtgccttttc atagtcgctt tcttttttca atggccgctc tgctcccggg gaggatacct
    39301 ccaggaaata agcttgagga atcggatctg gatctgctgc atcaagtttt tcactcagtt
    39361 tctcactgac aaaggcgcat tcttcgatat cgataccgcc ttctttgtca ataaatactc
    39421 gtaagaacca gctttttcct tcttttacaa attcgacttc gacaagttca aaattttgtt
    39481 catctaaaat cggtgtcacc atttctgtga ctgtttcaac gacgctactc aaaagtttcg
    39541 cctccttaaa aaattaagtg cgtaattcca ttaaaaagag tgagcgaaaa atccgctcac
    39601 tcacagttag tatcattacc tataagaact ataccatact ctgcttaaaa ttacaagcag
    39661 acaggaaaac agatgcttat agcatatcga ataacgataa ttgattttca tctggtagat
    39721 ctttcaatac gccattgtcg ttcatatatt cgatcaatgt ttttgaaact ttaccgcggg
    39781 tggcaagatc ttctttggat aaaaatggac cgtcttttct tgcttcaacg atttgttttg
    39841 ccacgttggt tcccaaactt gggacagctc taaatggtgc gatcaacgta tcaccttcaa
    39901 taacaaagtt aacggcatcc gatttataca aatcaatcat tccaaatttg aatcctcgtt
    39961 ccagcatttc attggctaac tccagcactg tcagttgatt cttttctttt acggaagcat
    40021 ctaatccttt gtccgtgatt tctttcattg cttgtttcac agcatctttt cctttgcaca
    40081 tggagactag atcaaagtcg tctgcacgta cagaaaagta tgcacagtaa tacaaaatag
    40141 ggaaataaac cttgaaataa gcgacacgaa gagccatcaa tacgtaagct gcagcatggg
    40201 ctttcggaaa catatatttg atttttgaac aagaatcgat ataccagtcg ggcacattat
    40261 tttctttcat cgccgataga taggtttctc gcaattcatc agggatcttg ttccattgtc
    40321 ctttacgaac agtttccatg attttaaatg ccatcccgct atctaatcca gcatggatca
    40381 aataaaccat aatatcatca cgacagccga ttacttccgc cagtgtcgca tctcctcgtc
    40441 gaatcaattc ttccgcattt cctaaccata cgtctgtacc atgagatagt ccggaaattt
    40501 gcagtagttc tgcaaaagtt gtaggatgcg tttcttctag catccctcga acaaaacgag
    40561 tgccgaattc tggaatacct aatgtccctg ttttggaata aatttgttcc tgacttactc
    40621 caagtacttc aggaccggca aatatccgca tcacttccgg gtcatccgta gggatcgttt
    40681 gtggatcgat ccctgataaa tcttgaagca tccgaataac agttgggtca tcatgcccta
    40741 gaatatctaa tttcaataca ttatcgtgaa tcgagtgaaa atcaaaatga gtcgttttcc
    40801 attccgaatt ttgatcgtct gctggatatt ggatcggcgt aaaatcatag acatccatat
    40861 aatcgggaat aacgatgatc ccccctggat gctggccggt tgtccgtttg actccagtag
    40921 cgccttttgc taatctgtcg acttctgcac ttcgaaactg cagattattg tccctttcgt
    40981 atccttttac atagccatat gctgttttgt cggcgactgt accaatcgtt ccggcacggt
    41041 aaacatagtc ttcgccaaac aatactttcg tatagttatg ggcttctgct tgataatcac
    41101 ctgagaagtt caaatcaata tcgggcactt tgtctccatg gaatcctaaa aatgtctcaa
    41161 atggaatatc atgaccgtct ttgtttaagc gtgcaccaca ttttggacat tgtttttctg
    41221 gcatgtcaaa tccagaacca tacgtaccat cttcaaaaaa ctcggaatat tggcactctg
    41281 gacaataata atgaggggct aacggattaa cttcagtgat tcctgtcatc gtcgcaacaa
    41341 agctagagcc aacagagcca cgtgaaccta ctagatagcc atcttcatta cttttatgca
    41401 ctagtttttg ggcgatcaga taaataaccg agaagccatt accattaata gaattcaact
    41461 ctttttttaa tcgtttttga atgatctcag gtaacggatc tccgtacatt tgttttgctt
    41521 ttgtgtaact caattctgat atttcatctt ctgaacctgg aatcttcggt gtataaagtt
    41581 catctttgac tggaatcact tcttcgcaga tatctgcgat tttattcgta ttttctacta
    41641 cgatctcttt cgctgtttct tcccctaaaa agtgaaatgc agttagcatc tcatcggttg
    41701 ttcggaaatg gacatccggc aaactatgac gattcaacgg attcgctccg cccatagaat
    41761 tgatcaaaat ttttcggtaa atggcatctt cttcgttcag ataatgtaca ttacctgttg
    41821 caacaacgat tttatccaaa ctttttccga tttctaccaa attttggata atttcttcca
    41881 aatcatgttc attttttacc aattcttgct cgatcaaagg cgcatagacc gcttttggca
    41941 taacttcaat ataatcgtag aattttgctc ggtttcttgc ctcttctacc cctttttgca
    42001 tcatggcttc gaatatttct cccttatcac aagctgagcc gatcaataga ttttcacgca
    42061 tctttttcaa ttgggagcgt ggtattcgtg ggacgcgttc gaagtattcg acattcgaca
    42121 ttgaaatcag tttgaacaaa tctttcagtc ctgcctgatt ttttgctaaa atcgttacat
    42181 ggaaaggtcg agcccttttg taagaatctc cttctccgat atgctcattt agctgatcat
    42241 gataaagcat gttatgattg tccattgcct ctttcacaaa gatccatgct aagtgaccag
    42301 ttgcttcagc atcatagatt gctcggtgat gctgttccaa gctgacacca aattttttag
    42361 ataacacacc taatccgaaa cgtttgaatt gaggatacag atatctagct aattccaacg
    42421 tatcaatgac tggattggcc gcttcaggaa taccatacct tgcgtagcta gtattcaaga
    42481 accccatatc aaaagccgcg ttgtgggcta ccaaaattgt atcttttgaa aattcaagga
    42541 acatccgcaa tacttcttct tccgactttg atcctcgaac cattccatcc gtgataccag
    42601 tcagatctac cgttgttcgt gacaaaggat gtcctggatc gataaattca tcaaagcttt
    42661 cgatcacatt tcctttatac attttgactg cggctagctc gatgatcgtg tcatatactg
    42721 cagacaatcc tgtcgtttcc acgtcaaata cgacatatgt ggcttcactc aatgcttcat
    42781 gtgcatcatt ataagcaatt gggacaccat catctacgac attcgcttcc acgccgtata
    42841 agattttcac acctgctttt tttccagcac tatgtgcttc cggaaaggct tgcgcaccac
    42901 cgtggtctgt aatggcgata gctcggtgtc cccatttccc tgcttgtgcc acaagatcag
    42961 agatgctgtt tgttgcatcc atcgtactca tattgctatg aacatgaagc tctactcgtt
    43021 tttccccttc aggagcataa tctttgcgag gtgtatgttt gacttctatg atatcttgcg
    43081 cattcatgac caaatcacgg acaaacgtat cttcttgaat acttcctcgt acctttaacc
    43141 agctaccgac actgatcgca tcaaatactt gttcatcttt ttcgccgttc gagaattttt
    43201 tgacgataaa tgatgaagta taatctgtga ttttcaaagt caagatcttg cgttttgaac
    43261 gtagttctct gacttctttg tcgaatacgt acccctcaag tgtgactctt cgttcttctt
    43321 caacgatact gatcattggt gtcgttggtt catcggctgg aatatttcgt cctagctgga
    43381 tgtggatggg tccttcaaga gctggcgatt gctcttttct ttcctttttc ttttgttcgt
    43441 ggacaactag actttcagct gcttgcttca taaaggcttc tgcctgttct tgtttgcgtt
    43501 cttcaaaaag tttcaatact cgttccgctt gctgctcatc cacttctggt tcgatacgaa
    43561 attttgggaa tccgtaactg acgaacaact cttctactaa tggcaagtat tgttgcttca
    43621 aataagaaat agctcctgta ctgtcgattg gcaagatgac tttacgatct tttatcacag
    43681 gtacctgcgt tttcaatact tgctgtgcta gtggtgtatc gcattgctga ttttccaaag
    43741 cttgggccca gtaatcttgc aagagctgtt catcgaatgt ctggtcatct gctaagatat
    43801 tcagtttgac ttgtgcaata tctttaaaag cgatagtcag atgctgagtc aatgaccgat
    43861 atagcataat aggcaaaatt ttggaaaaat gcagcgtaaa ttcccataaa cgcgatttac
    43921 gatggaccac tacactatcc atttcaccag cagtaagcag tggatgattt ttttctgctt
    43981 cctctagttg gatttgttcc aacagttttt caaataaatc tcgtttttct gacaaagata
    44041 tacgcccttt ctgatgagaa acaatgcaat tgatcgcatc ttatcctctt ctttttcagt
    44101 accttttagt atactcgttt tttctgagtt ttacattaaa aaattggaac tgcgattcaa
    44161 atcgcagttc caattttaaa agcgaatcat cagattgtca aaaacagctg attcagctat
    44221 ttttattttc tgtgttttaa cgcttattat tcgccttggt tcaagaggat tggtaaagta
    44281 ttcaccaatt cttctttacg tacttcaacc atttcgccag ttttcttgat tttcacttct
    44341 acgattccat ccactgcttt tttaccgaca gtgattctga ttggacagcc aatcaaatca
    44401 gaatccgcga atttcactcc tgcgcgttca tttctgtcat caaccagtac ttcatagcca
    44461 gcttttgtca tcgcttcttc tagttcattc gtcaggtttg tttggtattc gtcttttaga
    44521 ttcatttgga cgatatgcaa atcaaatggc gcaattcctt ttggccagtt gattccttgt
    44581 tcatctgaat gttgttctac gattgctgat aataaacgac ttacaccgat accataacag
    44641 cccataatca caaatttttc tcgtccattt tcgtctagta cagttgctcc catagattca
    44701 ctgtaacgag tgccgatttt gaagatatgt ccgatttcga ttcctcgagt aaaggcaaga
    44761 acaccattgt tgtctggtga aggatctcct tcttgtacaa aacgaagatc ttcataagaa
    44821 agtacagtga aatcgcgttc tggattcaca tttttatagt ggaatcctgt ttcgttcgct
    44881 ccagtaatgg cattcgctaa atcttggaca taacggtctg catatacttt tacgtcatct
    44941 gaaacaccga ctggtccgac agaaccaaac tcagcaccta gatatttttg tgcttcttct
    45001 tctgttgctt cttctaaaaa gtcagcagat aaaaagtttt tcagttttac gtcattgact
    45061 tcatggtctc cgcgtaccaa aacaaggaca ggttgttcat ccgcaatgaa caatacagac
    45121 ttgatgattt tttgaggttc aacagaaaag aaatctgcta cttcgtcaat tgattttacg
    45181 ttaggtgtag ctactttctc caattcaagc tgtgtttcat gcgatttttt tgatacatag
    45241 tagcttgtag ccatctccaa gtttgcagca taatcacttt cagtcgaata acagatcgta
    45301 tcctccccga tttctgagat cgccatgaat tcttttgagt ctttcccgcc cattgcgcca
    45361 ccgtctccga taatcgagcg aaattctaag ccacaacgct tgaatacttc ggtgtaggct
    45421 ttttcgtaat ctttatatgt ctcgtccaaa ctttcttccg ttgcatggaa agaatacgca
    45481 tctttcatga taaactctct tccgcgaagc aaaccgaagc gaggtcgttt ttcgtcacga
    45541 tatttaggtt ggatttgata aagatttaaa ggtaatttct tatatgaatt gatttcatta
    45601 cgcatcaaat cagtaaaagt ttcttcgtga gttggtccaa ggatcatttt tcgatcattg
    45661 cggtctttga aacgatacaa gtttggtcca tatgtttcgt aacgcccgga ttcttcccaa
    45721 agttctgctg gcaagatggc aggcatcagc atttctaccg ctccgatttt ttcaaattct
    45781 tcccgcataa tgcgtttcag tttttccaat acacgatttg ctaatggcag atatgaataa
    45841 atacccgctg atacttggcg gatatatcca gctcttagca agatttgatg gcttaatact
    45901 tctgcatcat ttggaacttc gcgtaatgtt ggaatcaaca tttttgattg tctcattcta
    45961 tatactcctt tatttcctca aaatagttat cgtttgttat tataccctat ttgtcaactt
    46021 tttctagaaa aagaatcgtt gaatatcgtt ccatgtaact agcaccatca acaacattaa
    46081 aaatccaaag ccagctaatg tcaatattcc ttctttttct tgacttaatg gtttcccgcg
    46141 aatcccttca aaaatattca agactaattt cccgccatct aaggcaggaa tcggaagcag
    46201 attgacgatt ccgagattca ttgaaagaag cgccatcaaa ctaataacag ttgttacacc
    46261 ttgatttgct gcttctgaag acaattggta catcatgaca ggtccgccta atttatctaa
    46321 actgaaacct gtaaatagtg atcctaaggc tttgaaaatt tccaatgaac cgctgaaagc
    46381 ttgacgcgtc cctccaatga tcttatccat aaaacctgta ttcattgggg cttttatacc
    46441 aagttggcca actttttcgc catttgactc tacagatttc ggtgtcacgt cgacagattg
    46501 cacctgacct tctcgttcga tcttaaagtc taatgtttta tccggatttt tcgtaatgac
    46561 tgttgtcaga tcattccatg aatggatttc ttttccgtcc acactgacta cctcatcatt
    46621 ttcttttaag cctgcttctg ccgcagctcc attaggcatg atctctccta cgcgattagt
    46681 gttcgtcact tgaacaccac cttgcataaa tgccagaatg atgaataaca ggattgccaa
    46741 aataaagtta ttcatgggtc cagcaaagtt ggtcagcata cgctgccaca atttagccga
    46801 ttggaactgt acgtcttttg gcgcaattct gatttctgtt ccgtcgtttt caataatcgt
    46861 cgcatcgtga tcaacagggt aggttactac ttctgtttcg tctccattca cataaccagt
    46921 gatagtcagc tcatcttcta aatcataacg gctcaattcc atcgggatag catttgtcaa
    46981 ttggattttc ttacttaaat ttatcttctc tacgatgccg tcagaattta gtaaaagaga
    47041 aagaggcatt cctggtgcca tttctgtttc atcatcccca ttgcctgcca ttcgaacata
    47101 accgccaata ggtaataggc gaagcgtata tgttgtcccg tcttttgctt gatgcccata
    47161 aattttcgga cccataccga tagcaaattc gcgaacgagg attcctgatc gtttcgcaaa
    47221 gaagaaatga ccaaactcat gaacaatcac taatatacca aaaacgatga taaatgtcag
    47281 aatcgttttc atagcttttt tctcctttac ttgttttttg tgttttttat aaaacaacga
    47341 taaaacacta taattttacc atagtatacc aaagacacca atagtatcaa tataatgaaa
    47401 aataaaaacc tgcgtctttt atgaaattca caaagaaacg caggtatact tactaaataa
    47461 tgccaaataa atgcatgata ggaaatacaa acagcaaact gtcgaaacgg tctaagattc
    47521 caccatggcc tggtaagata tttcccgagt ctttcacatc ataatgacgt ttgattgcag
    47581 attcgaccaa atcaccaaat tgtccgactg ctgaaaaaat aatcgtcatc agaagcatga
    47641 caaacatacc atgattaaat agttcttttg ctgggtatag caataaataa agaaatgcga
    47701 ctacaacagc actaccaata ccaccgagcg caccttcaat cgttttgttg ggcgaaatat
    47761 caggacttaa cttatgctga ccataacgtc gaccaaccat atatgctccg atatcagttg
    47821 cccaaacaat aaatagacca aatatcaaaa cagccaagct ttctgttcgt gcattgacaa
    47881 aattctggaa acctattccc acatacaagc tgacaagcac aggaaaacct gcttcatcaa
    47941 tcgtatacgt atttttagaa ataactgatg ttcctaaaag aatcataact ataagataaa
    48001 ataacataaa tgtacttaca ttctctggaa gaaagaaaaa ccatctttct tttggcaaaa
    48061 caagtaacac tgcaccaacg gcggataaaa ctccttcaaa acttaagagt gtcaatcctt
    48121 tcatccgaaa cagctcgtaa actcctatta ctgctaagat tgctgctgta agctcaaccg
    48181 ctatgcctcc gatccatata atcggaataa acaagactag cgccacggct gctgtgacca
    48241 cacgttgttt catgactgat cccctttctc atcttctgtt ttttttacac cgccgaatcg
    48301 acggtctcga ttttgataag aggcgattgc ttcttcaaga tgcgcaccat caaaatcagg
    48361 ccaaagcgct tttgtaaaat acagctcact atatgcgatc tgccataaca gaaaattact
    48421 aattcgctct tctccacttg tacggatcat cagctcagga tcttgtaact ctttaggcaa
    48481 gaaacctgtc attaaatgct tagcaatcaa ttcatcatcg atttcttctg cgtgtatttc
    48541 tccttttgct acttcttcgg caatttcttg gacagcagtc acgatctctg ctctgcttcc
    48601 ataattcaaa gcaaaattca ataccattcc agtattgttt tttgtttgct caattgcacg
    48661 tcttacagca tcttgcgtat gttctggcaa tacgttttca taacccatga catgaacttt
    48721 gacattttct ttaatcaatt ctggtacgaa cgtatcaaag aagtcgacag gtagttgcat
    48781 gagaaaactt acttcatctt ttggtcgctt ccagttttca gtcgaaaagg cataaagcgt
    48841 caacactttc actccaagcc ttgatgcttt ttttgtgact ttctttactg tttccatgcc
    48901 ttctttatgg ccagcaactc gtggcaaacg acggttttgc gcccaacgac cattgccgtc
    48961 catgatgatg gcaatgtgct gaggaattgg tccatcggta ttaaattgaa attcgctttt
    49021 ttcttgaata tatttgtttt tttgcggaaa aaaacgtaac attctttttc ctcctatact
    49081 taaaagattc ccactatctt aagtattata gcaatatcta actgtttttc ctatgaaatc
    49141 attgtttttt tatttaaaaa ctgaataatt ttttcttttt ttaattttta aataaaagaa
    49201 atataaataa aaacagagga agcggaatga tccgcccctc tgtttttatg attttattaa
    49261 acttccaata attctttttc tttatcagca gcgatcttat ctacttcttt gatgctgtta
    49321 tctgtcaatg tttgtacgtc tttttctaat ccgcgcaaat catcttcggt aatatcacca
    49381 tttttttgtt gttttttata ttcatcgatc gcatcacgac ggatgttacg aatcgcgatt
    49441 tttgcatttt ctgcttcttt tttaacatct ttagctaatt cttttctgcg ttcttctgtc
    49501 agttgcggga taactaagcg aatgacattt ccatcattgg ttggactgat tccgatatca
    49561 cttgccataa tagctttttc aacatcttga agaatgctct tgtcaaaagg tgtgatcatt
    49621 aaaacacgtg cttctggaat ttggattgat gccatttggt tcaatggtgt tggcgcaccg
    49681 tagtagttca cagtaatacg gtccaaaagg ctagcgttcg cacggcctgc gcggatttgc
    49741 ccaagttcac gttgaagatt ttgtgctgct tttttcattt tttcttttgc ttctgtcata
    49801 attgcatctg ccatttttat ttccccctta cagttgttcc gatattttcg cctaagattg
    49861 cacggcgaat atttccgtgt tcatttaaat tgaagacaag cagtggaata tcattatcca
    49921 tgcttagtga acttgcagtt gaatccatga cttgtaatcc tttggcaata acttccagat
    49981 gagtcagttc ttcaaatttt actgcatttg catctacttt cggatcagca gaataaacac
    50041 cgtctacatt gtttttagcc atcaggataa catctgcgcc gatttctgcc gcgcgtaatg
    50101 ctgctgttgt gtctgtcgag aagtacgggt tgccggtacc gcctgcaaag ataacgatcc
    50161 gtcctttttc taaatgacgt tctgctttac ggcgaatata tggctctgcg atttgacgca
    50221 tttcaatcga agtttgcacg cgtgtcggta cacccacgtt ttccaacgta tcctgtaaag
    50281 ctaaggcatt cataactgta gctaacatac ccatgtaatc agcttgagca cgttccatgc
    50341 ccatttgtgc tccgatttgt ccgcgccaga tatttccgcc gcccacaacg attgccattt
    50401 caacgcccag ctcgtgaact tctttgattt cttggatgat ttctttgatt actggtggtt
    50461 tgattccaaa tcctgcgtcc ccagctaaag cttctccact caattttaag actacacgct
    50521 gatatttcgg tgtgaccatg ttcattcctc caatattttc tattgccatt ttaccatact
    50581 tagatgattt cgacactcta agactggttt ttcttgtttt gcttaaaaaa agggagcgca
    50641 caaaccgtgc gttcccgtct gttattcaac agaattattt tttcacttgg ctcataactt
    50701 cttctacaaa gttatcttcg cgtttttcga taccttcgcc aacttcgaaa cgtacgaatg
    50761 ttttaacagt tgcgcctttt gatgcaacgt atttttcaac agtcatatca ggatctttaa
    50821 cgaatggttg gtccaccaaa gcgatttctg ctttgaattt cttcaagcgt ccttctacca
    50881 ttttttcaac gatattcgct ggtttgcctt cgttcaatgc ttgttctgta agaacagttt
    50941 tttcatgttc taattcagct tctggaattt gtgattcgtt tacataacga ggattgatag
    51001 ctgctacgtg catagctacg tctcttgcaa ctgtttcgtc agttgttcca tctagaacag
    51061 tcaatacggc aatacgtccg cccatatgca agtatccacc aaatgcagca ttgtcgtctt
    51121 tttctacaac ttcaaagcga cggaagctga ttttttctcc gataacttga gttgcttcga
    51181 tcaagtcaga ttcaattgta cctttatctg ttttgatttt cattgcagct tccatgtctg
    51241 ctggtttgtt ttcagcaaca agttctgcaa tttcttttac taaatcttgg aacatttcgt
    51301 ttttagaaac aaagtctgtt tctgagttta cttcaacgat tgctgcagtg ttacctttca
    51361 cagcaactga agccaatcct tcagctgcaa tgcggtcatt tttcttcgca gcctttgcca
    51421 ttcctttttc acgtaaaaga tcaactgctt tttccatgtc gccttctact tcaactaatg
    51481 cttttttcgc atccatcatt cctacgccag tcatttcgcg tagttctttt accattttag
    51541 ctgtaacgtc tgccatgttt aactcctcct gtgtgttttt ctttttaaaa aaagctgctt
    51601 caaaggagag tgaggcttgt cgcccagcct ttgaaacagc tccatcattc aaaattattc
    51661 tgctgaagaa ttgtcgcctt caacaacatc tacgatttct tcgattgatg ttgctgtgtt
    51721 ttcttctgtg aaatcttctt caactacttg atcttcacct tggtttcctt cgatgaaagc
    51781 atcagccatt tttgaagtaa tcaatttaac ggcacgaatt gcatcgtcgt ttgaagggat
    51841 tactacgtcg atttcatctg gatcgcagtt tgtatcaacc atagctacga ttgggatatt
    51901 taatttgtgt gcttcttgaa cagcaatacg ttctttacgt gggtcaacga tgtacattac
    51961 atctgggatt cttggcatat cagcgatacc gcctaagaat ttttcaagac gttcacgttc
    52021 tttgttcaaa ccagcaactt cttttttagg tagaacttca aaagttccat cttcttccat
    52081 tgcattgatt tgtttcaaac ggctgatacg tttttggata gtatcccagt ttgtcaatgt
    52141 tccgcctaac caacgatggt taacgaagta ttgtcctgca cgagttgctt cttctttgat
    52201 tgcttcttgt gcttgttttt ttgtacctac gaataatgct acgccgcctt cttcagcaac
    52261 gctcttcatg taatcataag ctgcatctac taatttaact gttttttgca agtcaatgat
    52321 gtagattccg tttctttctg tgaagatata tttcttcatt tttggattcc agcgacgagt
    52381 ttggtgacca aagtgtacgc cggcttctag taattgtttc atagaaatta ctgccatgtt
    52441 tgtttcctcc gatttggttt tatttttcct cttcttatct tctaccttgc cggcaaactt
    52501 tttcaagcac ctaccgacaa tccaataaga atgtggattt gctgatgcat aaagcaccct
    52561 cagctattat acagacaaaa gatccgtatc gcaagagcaa atatactttt ctgctcaaca
    52621 ttcaatagtt tacctgaaag ataaaaagaa ttcaagaaaa acagtagatt tctctttgtt
    52681 tctttattat cctttttcca taaaataatt ttaggtataa aagacgaata ttattgggca
    52741 atccttcttt tttttgttat gatacatgtg aaatctttcg taaaggagaa agaaaatgcg
    52801 caaagctcaa agacatttgc tgattaaaca agtcattgaa gaattcccga tccgtacgca
    52861 agaagaacta ttaaacaaac ttgggcatta tggcgttacc gccacgcagg caacgatttc
    52921 tcgagatatc cgtgatctaa aaatcgtgaa ggcacctgat gaaaatggcg tttcccgctt
    52981 cgtattgttt cgcgaaaaag gaactcctga gactaggact gaggaagagg atcggctggt
    53041 tcaaatgatt gaagacattg tattgaaagt cgaacgagtc cactttttga cgatcgtcaa
    53101 taccttgcca gacaatgctc atttatttgc atctgtttta gatgagatca aacctccctc
    53161 tattgtcagt acaattgctg gattcgatac gatcatggtc atctcggaaa atgaggagga
    53221 cgcaaaacga gtagaagaat ttcttcacca tccaactgaa gtatcacttt tttaatcaga
    53281 gcacgtattc ctaatatgga atgcgtgttt ttttattttc aatattttcc ttattgaata
    53341 aacctttaca ctctttacaa aaatacatca ttttttattc aaatttttca acaccttgat
    53401 tgaatcacaa taatcatcat ttatattgga tgttaacaag agcagcttct tgctctttgt
    53461 gtatttcaaa aaagaaatga aaggaaatca tatgtataaa ttattaactt ctggaccatt
    53521 gacgacttct gaaacagtga aaaatgctat gctgatcgat cattgtactt gggatcaaga
    53581 atacaaagaa attacacaat ggataagaaa aaaattattg gatcttgctc aagtatcgga
    53641 aggaatatac acaacggtct taatgcaagg tagcagctcc ttcggggtag aatcggtatt
    53701 aagttctgtg atcaggccag aagatactgt actgatttgt tcaaacggcg catatagaca
    53761 acgaatgatc aacatgtgcg aatgtctgaa actgaactat accgtttatg aggtatcgga
    53821 acatcaaatc ctccaagctc atgtgattac acagctgatt gaagagaatc cgtctatctc
    53881 cgcactagca atggtccata gcgagactac ttccggtatt ttgaataatt tggaagagat
    53941 tgctgctgtc acacaagaaa gagatctttt gtttatagtg gacgcgatgt caagctttgg
    54001 tgggataccg atttgtgtag ccgaactagg gattgatttt ttgatttcca gctcaaacaa
    54061 atgtattcaa ggtgttccag gcttttcttt tgtacttgct aataaagcgg aactggaaaa
    54121 acgaaaaaat gttagccgaa gtttgtcact tgatctatat gaccagtacc gcgtgatgga
    54181 aaacgagaat ggcaaatggc gtttcacttc ccctacccat acggttttgg cttttcaaca
    54241 agcgctgaag gaactagagg cagaaggagg aatcgaaact cgatattcac gttatctcgc
    54301 caataatcat gtgctacgac aaaaaatgac agagatgggg ttccagcctt tcattcaaga
    54361 atatcagggg ccattcatca cctcttttct ttaccctaag gactggcgat ttgattttaa
    54421 tcatttttat cagtatttaa agcaaaatgg atttgtgatc tatcccggga aaatcagcga
    54481 tcatgaatgt tttcgtatcg gaaatattgg cgagatctat ccggaagata ttttggaact
    54541 gacagcaatt atcaaagact ataaggagaa cgttaatgat taaaactgta atttttgatt
    54601 gggcaggaac aactgtggac tttggctgta tggctcctgt ccacgctttt agaaatgctt
    54661 ttctagaaaa aggaatccaa ctgactgaca aagaaatccg ctaaccgatg ggaaaattaa
    54721 aatgggatca tatccaacag cttctttccc tcccttctgt caaaaaacaa tggatagcta
    54781 tttatggaca actacctcag gaaaaagagg tggaggaatt gtatcaacaa tttgaacatt
    54841 atctgtttaa agatttggtc aagcattcta ccttgaaacc cgacacattg gaaacgatcg
    54901 aacggttgag aaacgctggg atcaatattg gttccacgaa tggatttaat gctcctatga
    54961 tgacaatcgt agcagaaacc gcaaaaaaag aaaggtattc accagacttc atggcaactt
    55021 ttgaagacgt cgaagggtat ggtcgaccgt ttccttatat gatccataaa aacatgcagt
    55081 acttcaaaaa caagtcggtt gatgaagtaa tcaaagtcgg agatacggtc tcagatatcc
    55141 aggaagggaa aaatgccgga gtgttgacag taggtgtaat tgaaggtagt tcgctaatgg
    55201 ggatttctta tgatgagtac caaaaattag agatggggaa aaagatcgtt taaaacagtc
    55261 tgttgctctt agattccaac aagccggcgc tgatatttgt gtaaacaatt taaaagagtt
    55321 aacagaatac attttgtacg ctgacatgaa tccagaatga cttttataca aaataagagg
    55381 ttgtgaaaaa gctgtcctgt atcaagtaat aaaaacagcc aagcaaaaat agctcctcat
    55441 atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattcga
    55501 aaaaaaaaag ggagctgtga cacgactttt gtcacagtcc ccttatttgc aaacaaacag
    55561 tgtttaagag acaacctctc cactaaacgg tgttcattca gctgactctc ggtatttgct
    55621 caaaaaacga taaaatttga aaagctattt tctcttttgt ttctctaata ctcggatcag
    55681 aacgctgatt tcacattctc tttttcttac atcttatctg gtgcatgaag tcctagcaaa
    55741 cgaaggtctt ctttcaataa tacagtcact gcataaacta gagctaagcg tgcttctttt
    55801 tgttcatcat cagctaaaat cttcgtatga gcatagtatt tgttgaatgc ttgtgctaat
    55861 ttgattgcat gtttcgcaat cactgacggt tcgtattttt ccccagcaga caagaccgtt
    55921 tcagggtatt tttgaactaa tttaaccact tcccagctgg tatcgtcatt caatgcatag
    55981 tctgctgttt ctgaagggac aaatcctgct ttttccaaca ggctgactgc acgagcatgt
    56041 gtatattgca cataaggtcc agtttctcct tcaaatcgaa caacttcttc caggttaaag
    56101 tcaaatgtat tcagtcgatc gtttttcaaa tcgtggaaaa tgactgcccc aaccccaact
    56161 tgtttagcta cagcatcttt gttttccaaa tcagggtttt tctcgctgat ttgttcttta
    56221 gcagattgga ttgcttcatt taatacttct tctaataaga cgatctttcc tttacgagtt
    56281 gataattttt taccaccttg cgtgatcaaa ccaaatggaa tatggtgcat gtcgtcagac
    56341 cagtcaaagc ccatttcctt caagacagct ttcaactgtt taaaatgata gctttgttca
    56401 ttccctacta catatagaga ttgtttgaag tcgtaagtac gtttacggta caatgcagca
    56461 gctaagtcac gtgtaatgta tagtgtcgca ccgtctgact tcttaatcaa cgctggattc
    56521 aggtcataag cagataggtc aacgatctct gcacctttat cctcgttcag caaatgtttt
    56581 tcttctaaaa gtttgacgat ttcatccatc ttgtcattgt agaaagcttc accgttcaac
    56641 gaatcgaaac gtacttccag cagatcatag attttattga attctttcat cgattcatca
    56701 cggaaccatt gccataattg aatagcctct ttatcgcctt cttccagacg tttgaaccaa
    56761 gcacgagctt cttcatttaa ttctggttct gtttcagcta cttcatggaa ttggacgtat
    56821 aaacgaagca attcattgat tggttctgct ttaacagctt cttctgtacc ccattttttg
    56881 taagcgacga tcagttttcc aaactgtgtt ccccagtcac ctaaatggtt gatgcggatt
    56941 ggctgataac cgattttttc cataatgaaa ccgatcgaat taccgataac tgtcgaacgc
    57001 aaatgtccca ttgagattgg tttggcaata ttaggagaag acatgtcgat tggtactgtt
    57061 ccttgattcc cgatgttgct atccccataa tgttcttttt ctttcactac tgtttgcaaa
    57121 acttttttgc tgataagttc tttattcatg aaaaagttca agtaaggtcc gacaacttcg
    57181 attttttcaa agttggcaga atcgattttt tctgctaaat ctgcagcgat ttgctgtggc
    57241 gcttttcggt aaacttttgc taatgaaaag gctggaaatg caacatctcc gtgttcagct
    57301 gattttggat tttctagtaa ttgctcgact tgttccagtg ttaaatcatc tttgacagcg
    57361 tcatagacag cttttgcgac aagatcttta ttattcatct ttattcctcc taaagtgttc
    57421 ccttgattgt taaacaaaaa aaatctctag cgacggcata cttagtatgc catcactaga
    57481 gacgagattt cccgcggtac cactccaatt gtcctaaaaa aggaccagct ccatctgata
    57541 tcggtagacg accgtctgtt taaaacagaa ttctccaagt gcgcttcgtt gtatcttctt
    57601 cattcggctt ccaccacttc cgaattcgct agccaagaat gatacaagta ctctcttgtt
    57661 caacaaatct tagtcttatt aaactttctt gaattatatc agaaaaaaaa acgaaaggct
    57721 agtcgtttca taaaaaaaca atccagatgg cctaaaggag aagaaatacc acctggatag
    57781 ctataaatca tttcgtaaat acgaaagact taacaaatta atcagctacg ataatcgttc
    57841 cttcttcaga agctaaaaga ttctcgatat tttctaaaga agtgatgatt gccttagcgt
    57901 ttggttttgc ttcaacaaat gcaatagctg cttctacttt cggaagcatg cttcctggag
    57961 cgaattgctt ttcatcaatg tattgtttca tttctgaaac agtaacagtt tctaatttct
    58021 tttgatctgg tttttggtaa ttgacataca cattgtctac tcctgtcaat acgatcagca
    58081 aatccgcatc gatgatttcg gctaattttt ctgaagcaaa atctttatcg attacggctt
    58141 ctcgtccttc taatccagta gctgtttcta caacaggaat cccaccgcca ccaacagaga
    58201 ctgtgataac tccttgatcg accagcgttt cgatgacacg tgcttcttta atcgagatgg
    58261 gtttagggga agcgactact tttctccatc cacgtccagc atcttctttg aatgtcacag
    58321 tggagtcagc attcatttgt tctttggctt cttcttccgt gtagaatgga ccgactggtt
    58381 tacttggatt tttgaatgaa ggatcatttt catccacaat aacttgagtg actaaagaaa
    58441 ccacatcttt atcgatccct ttttccttca gtacttcgcc catggcgttt tgtaaccagt
    58501 aaccgataga tccttctgtc attgcaacac atgtatctaa aggcattgcc ggtgttttct
    58561 cagaatcagc agcttgctgc tgaatcaata ggttaccgac ttgcggacca tttccgtggg
    58621 agatgatcaa ttcatctcct tgttcgataa atttcactaa atactttgct gtttccatta
    58681 acgcttcttg ttgggctttt gcacttgcat ccgtagacaa gatggcattg ccccctaaag
    58741 cgacaactac ttttcgtttg gccatttcat tcgtcctttc ttcagttcta ccgaattttc
    58801 tagataacaa aacgagccag cgttttgtga ttttccctca atgctgactc gttttttcgt
    58861 gagatgaatg tggttatgct taaacgcgag ggataaacaa gttacctaat gttgctgcca
    58921 tgatcgcttt gattgagtgc atacgatttt ctgcttggtc aaattgacga gcatatttgc
    58981 tgcggaagac ttcatctgtt acttccattt cagtgatgcc aaagcgttct ttcatttgtt
    59041 caccgtaaac agtgtttgta tcatggaaag ctggcaagca gtgcaagaag atcaaacgat
    59101 ctgtgttgtg tgttttttct accatttcca tattgatttg gtaaggtttc aacaatttga
    59161 tacgttcttc gaatttatct tcttcaccca ttgataccca tacatcagaa taaaggacgt
    59221 ctgctccgtc tacaccttta tcaacatcgt cggtaatcat caattgtgca ccggattttt
    59281 cagcaaagcc ttctgccatt ttgacgattt cttcttcagg ttgtaattct tttggtgcaa
    59341 cgatacgcat attcgctcct aaaattgcac ctgtgactaa taaagagtta gccatattgt
    59401 ttcgaccatc tccgcagtaa gcaacagtga taccttctac tgttccaaag ttttcttgga
    59461 tagttaaaaa gtcagcgatc atttgtgttg gatgccattc atcagtcaag ccattccaaa
    59521 ctggaacacc agagaattct gctagttcct ctaccatttt ttgactgaat ccacggaatt
    59581 cgattccgtc aaacatacgt cctagtactt tagctgtatc ttctgtggat tcttttttac
    59641 ctaattggat atcatttgca cctagatatt caggatgtgc acctagatcg atcgctgcag
    59701 tagtaaatgc agaacgagta cgtgtagatg ttttttcaaa taaaagcgca atattttttc
    59761 cttctagata gtgatgtggg ataccacgtt ttttcaagtc tttcaagtgt tctgaaaaat
    59821 cgatcaaata ttgtaattct tcttttgtaa agtctttttc tgctaataag cttcttcctt
    59881 ggaatactga ttctttcata ttaaacattc tcctttagat tgtttttatt taagattaaa
    59941 gaggaaatcc cgacggcagc tgacagtaag actgccgtca ggaaacaaat tacttcaagt
    60001 cttcacggac aagtggttgg ctcatgcagc gtggaccacc acgacctcgt gacaattcac
    60061 ttgaattgat ttctaacact ttgatgccat agctacgaag caattcgttc gatacataat
    60121 ttcggttata agtgactaca acacctggag cgattgccaa tgtgtttgat ccatcattcc
    60181 attgttctcg aggtgcaacg atctcatctc cattaccagt tgggatcagt acaagatcat
    60241 ctaatccaag agcagctttc atcgtttcgt gtaagttatc tgaatgagta atcttgatat
    60301 catcaccatc tggttcgatt gtaaagacat ccatcttacc gtctttactt tgaattgctg
    60361 gatggatagt gaatttatcg tgatctacca ttgtaaatac ggtatccaaa tgcatcattg
    60421 cacggttgtt cggaattttg attgccaaca ctttttcaaa tcctgaattt tttgcgaata
    60481 ggtggcgtgc taatttttct aaagctttcg cattagttcg ttgagaaatc ccaacagcta
    60541 ctactttatc gcttaagatc aattcgtctc cgccttcgat atgatctgga ttttcacgat
    60601 ccaaccatac ttcgattcct ttatttgcaa atcttggatg atgtttcaaa atatattctg
    60661 taaacatcga ttcacgacga cgtgcttcaa aagtcatctt attgactgtc atcccatttc
    60721 ccattgatgc ggaagggtcg cgtgtaaagt acaagtttgg cattgggtcc atgtaaaatg
    60781 gatagtcatc gtctgctgac agatcatata gactgctaga acgtacttcg atatcttttg
    60841 tacgaacccc agccatgatt ttatctacca tttcttgcgt ttccattgat agaaggaact
    60901 catgaagacc ctctcttaca gcattcgatg caatatgaga ttcatccaac attttattca
    60961 agaattgttc tttgacattg cctgcgtcaa tcgcttctgc cgctaatttt tccaagtaaa
    61021 gagtctcgac gccttcattt tgcaatgtct tcgcaaagtt gtcatgttct tcttgagcaa
    61081 tcggaagata tggaatatca tcgaacaaca gacgatccat aatatctggt gtcaaatttt
    61141 ccacttcttg tccaggccgt ttcagtaaaa ctgtctttaa tttccctatt tcagagaaaa
    61201 cgtgaatagg tttatccatg ttgtgttgcc tccttctttt tgattacatg tataggatat
    61261 cctccttttg taatcacttt cataattttg tctcattcaa aatgagacat tcctcacatt
    61321 aatattcgat aattaaataa ttaaacgttt ttctattttt aagcg
//
LOCUS       DAJSBN010000011        60193 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJSBN010000011 DAJSBN010000000
VERSION     DAJSBN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.011"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="ONT86_000766"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="ONT86_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR2351581.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ONT86_000767"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ONT86_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR2351582.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="ONT86_000768"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="ONT86_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR2351583.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="ONT86_000769"
     CDS             1642..2274
                     /locus_tag="ONT86_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR2351584.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="ONT86_000770"
     CDS             complement(2276..3181)
                     /locus_tag="ONT86_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR2351585.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="ONT86_000771"
     CDS             complement(3266..3925)
                     /locus_tag="ONT86_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR2351586.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="ONT86_000772"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="ONT86_000772"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCR2351587.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="ONT86_000773"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="ONT86_000773"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCR2351588.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="ONT86_000774"
     CDS             6168..7253
                     /locus_tag="ONT86_000774"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCR2351589.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="ONT86_000775"
     CDS             7250..8314
                     /locus_tag="ONT86_000775"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR2351590.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="ONT86_000776"
     CDS             complement(8563..9366)
                     /locus_tag="ONT86_000776"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCR2351591.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ONT86_000777"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ONT86_000777"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR2351592.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="ONT86_000778"
     CDS             complement(10244..11251)
                     /locus_tag="ONT86_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR2351593.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="ONT86_000779"
     CDS             complement(11258..11902)
                     /locus_tag="ONT86_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR2351594.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="ONT86_000780"
     CDS             complement(12002..12760)
                     /locus_tag="ONT86_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR2351595.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ONT86_000781"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ONT86_000781"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR2351596.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ONT86_000782"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ONT86_000782"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR2351597.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ONT86_000783"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ONT86_000783"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR2351598.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ONT86_000784"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ONT86_000784"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR2351599.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ONT86_000785"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ONT86_000785"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR2351600.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="ONT86_000786"
     CDS             18158..19027
                     /locus_tag="ONT86_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCR2351601.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="ONT86_000787"
     CDS             complement(19153..20052)
                     /locus_tag="ONT86_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2351602.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="ONT86_000788"
     CDS             complement(20217..21041)
                     /locus_tag="ONT86_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR2351603.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="ONT86_000789"
     CDS             complement(21052..22347)
                     /locus_tag="ONT86_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCR2351604.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="ONT86_000790"
     CDS             complement(22363..22842)
                     /locus_tag="ONT86_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR2351605.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="ONT86_000791"
     CDS             complement(22848..23876)
                     /locus_tag="ONT86_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR2351606.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="ONT86_000792"
     CDS             complement(23893..24999)
                     /locus_tag="ONT86_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR2351607.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ONT86_000793"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ONT86_000793"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR2351608.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ONT86_000794"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ONT86_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR2351609.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ONT86_000795"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ONT86_000795"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR2351610.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="ONT86_000796"
     CDS             29970..30668
                     /locus_tag="ONT86_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR2351611.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="ONT86_000797"
     CDS             complement(30836..33475)
                     /locus_tag="ONT86_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2351612.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ONT86_000798"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ONT86_000798"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR2351613.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="ONT86_000799"
     CDS             35318..36493
                     /locus_tag="ONT86_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2351614.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="ONT86_000800"
     CDS             36497..37138
                     /locus_tag="ONT86_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2351615.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="ONT86_000801"
     CDS             37135..38052
                     /locus_tag="ONT86_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2351616.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="ONT86_000802"
     CDS             38049..38720
                     /locus_tag="ONT86_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2351617.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="ONT86_000803"
     CDS             complement(39261..39749)
                     /locus_tag="ONT86_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCR2351618.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="ONT86_000804"
     CDS             complement(39950..40216)
                     /locus_tag="ONT86_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR2351619.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="ONT86_000805"
     CDS             complement(40216..40479)
                     /locus_tag="ONT86_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2351620.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            40955..41140
                     /locus_tag="ONT86_000806"
     CDS             40955..41140
                     /locus_tag="ONT86_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2351621.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41282..43105)
                     /locus_tag="ONT86_000807"
     CDS             complement(41282..43105)
                     /locus_tag="ONT86_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCR2351622.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="ONT86_000808"
     CDS             complement(43703..46408)
                     /locus_tag="ONT86_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCR2351623.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46714..47661)
                     /locus_tag="ONT86_000809"
     CDS             complement(46714..47661)
                     /locus_tag="ONT86_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCR2351624.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47851..48120
                     /gene="rpsN"
                     /locus_tag="ONT86_000810"
     CDS             47851..48120
                     /gene="rpsN"
                     /locus_tag="ONT86_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR2351625.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48296..48658)
                     /locus_tag="ONT86_000811"
     CDS             complement(48296..48658)
                     /locus_tag="ONT86_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR2351626.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48669..49790)
                     /locus_tag="ONT86_000812"
     CDS             complement(48669..49790)
                     /locus_tag="ONT86_000812"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR2351627.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49806..50156)
                     /locus_tag="ONT86_000813"
     CDS             complement(49806..50156)
                     /locus_tag="ONT86_000813"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR2351628.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50286..51797)
                     /locus_tag="ONT86_000814"
     CDS             complement(50286..51797)
                     /locus_tag="ONT86_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR2351629.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52119..53471)
                     /locus_tag="ONT86_000815"
     CDS             complement(52119..53471)
                     /locus_tag="ONT86_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR2351630.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53624..54700)
                     /locus_tag="ONT86_000816"
     CDS             complement(53624..54700)
                     /locus_tag="ONT86_000816"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR2351631.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54896..56641
                     /locus_tag="ONT86_000817"
     CDS             54896..56641
                     /locus_tag="ONT86_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCR2351632.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56921..57358)
                     /locus_tag="ONT86_000818"
     CDS             complement(56921..57358)
                     /locus_tag="ONT86_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR2351633.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57342..58715)
                     /locus_tag="ONT86_000819"
     CDS             complement(57342..58715)
                     /locus_tag="ONT86_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351634.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59037..59525)
                     /locus_tag="ONT86_000820"
     CDS             complement(59037..59525)
                     /locus_tag="ONT86_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2351635.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59547..60149)
                     /locus_tag="ONT86_000821"
     CDS             complement(59547..60149)
                     /locus_tag="ONT86_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR2351636.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17104 a        13016 c        10989 g        19084 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    46561 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    46621 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    46681 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    46741 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    46801 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    46861 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    46921 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    46981 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    47041 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    47101 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    47161 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    47221 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    47281 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    47341 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    47401 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    47461 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    47521 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    47581 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    47641 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    47701 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    47761 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    47821 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    47881 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    47941 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    48001 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    48061 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    48121 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    48181 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    48241 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    48301 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    48361 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    48421 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    48481 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    48541 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    48601 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    48661 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    48721 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    48781 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    48841 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    48901 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    48961 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    49021 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    49081 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    49141 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    49201 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    49261 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    49321 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    49381 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    49441 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    49501 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    49561 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    49621 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    49681 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    49741 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    49801 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    49861 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    49921 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    49981 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    50041 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    50101 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    50161 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    50221 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    50281 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    50341 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    50401 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    50461 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    50521 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    50581 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    50641 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    50701 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    50761 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    50821 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    50881 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    50941 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    51001 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    51061 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    51121 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    51181 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    51241 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    51301 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    51361 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    51421 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    51481 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    51541 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    51601 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    51661 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    51721 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    51781 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    51841 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    51901 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    51961 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    52021 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    52081 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    52141 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    52201 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    52261 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    52321 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    52381 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    52441 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    52501 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    52561 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    52621 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    52681 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    52741 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    52801 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    52861 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    52921 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    52981 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    53041 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    53101 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    53161 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    53221 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    53281 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    53341 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    53401 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    53461 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    53521 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    53581 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    53641 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    53701 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    53761 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    53821 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    53881 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    53941 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    54001 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    54061 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    54121 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    54181 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    54241 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    54301 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    54361 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    54421 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    54481 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    54541 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    54601 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    54661 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    54721 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    54781 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    54841 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    54901 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    54961 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    55021 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    55081 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    55141 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    55201 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    55261 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    55321 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    55381 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    55441 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    55501 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    55561 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    55621 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    55681 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    55741 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    55801 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    55861 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    55921 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    55981 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    56041 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    56101 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    56161 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    56221 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    56281 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    56341 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    56401 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    56461 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    56521 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    56581 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    56641 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    56701 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    56761 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    56821 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    56881 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    56941 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    57001 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    57061 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    57121 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    57181 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    57241 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    57301 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    57361 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    57421 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    57481 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    57541 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    57601 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    57661 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    57721 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    57781 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    57841 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    57901 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    57961 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    58021 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    58081 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    58141 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    58201 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    58261 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    58321 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    58381 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    58441 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    58501 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    58561 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    58621 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    58681 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    58741 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    58801 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    58861 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    58921 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    58981 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    59041 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    59101 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    59161 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    59221 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    59281 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    59341 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    59401 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    59461 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    59521 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    59581 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    59641 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    59701 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    59761 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    59821 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    59881 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    59941 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    60001 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    60061 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    60121 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    60181 aaactgcctc ctt
//
LOCUS       DAJSBN010000012        57954 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJSBN010000012 DAJSBN010000000
VERSION     DAJSBN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.012"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            95..673
                     /locus_tag="ONT86_000822"
     CDS             95..673
                     /locus_tag="ONT86_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR2351637.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            673..1602
                     /locus_tag="ONT86_000823"
     CDS             673..1602
                     /locus_tag="ONT86_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2351638.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1599..3335
                     /locus_tag="ONT86_000824"
     CDS             1599..3335
                     /locus_tag="ONT86_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2351639.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3398..4234)
                     /gene="pheA"
                     /locus_tag="ONT86_000825"
     CDS             complement(3398..4234)
                     /gene="pheA"
                     /locus_tag="ONT86_000825"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCR2351640.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4231..4740)
                     /locus_tag="ONT86_000826"
     CDS             complement(4231..4740)
                     /locus_tag="ONT86_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCR2351641.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4733..6028)
                     /gene="aroA"
                     /locus_tag="ONT86_000827"
     CDS             complement(4733..6028)
                     /gene="aroA"
                     /locus_tag="ONT86_000827"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR2351642.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(6041..7132)
                     /locus_tag="ONT86_000828"
     CDS             complement(6041..7132)
                     /locus_tag="ONT86_000828"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCR2351643.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7149..8315)
                     /gene="aroC"
                     /locus_tag="ONT86_000829"
     CDS             complement(7149..8315)
                     /gene="aroC"
                     /locus_tag="ONT86_000829"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR2351644.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8317..9381)
                     /gene="aroB"
                     /locus_tag="ONT86_000830"
     CDS             complement(8317..9381)
                     /gene="aroB"
                     /locus_tag="ONT86_000830"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR2351645.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9414..10436)
                     /gene="aroF"
                     /locus_tag="ONT86_000831"
     CDS             complement(9414..10436)
                     /gene="aroF"
                     /locus_tag="ONT86_000831"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCR2351646.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(11095..12069)
                     /locus_tag="ONT86_000832"
     CDS             complement(11095..12069)
                     /locus_tag="ONT86_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCR2351647.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(12050..13933)
                     /locus_tag="ONT86_000833"
     CDS             complement(12050..13933)
                     /locus_tag="ONT86_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HCR2351648.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(13951..16176)
                     /locus_tag="ONT86_000834"
     CDS             complement(13951..16176)
                     /locus_tag="ONT86_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR2351649.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            16291..17325
                     /locus_tag="ONT86_000835"
     CDS             16291..17325
                     /locus_tag="ONT86_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2351650.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(17392..18405)
                     /locus_tag="ONT86_000836"
     CDS             complement(17392..18405)
                     /locus_tag="ONT86_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2351651.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(18483..18671)
                     /locus_tag="ONT86_000837"
     CDS             complement(18483..18671)
                     /locus_tag="ONT86_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR2351652.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(19111..19239)
                     /locus_tag="ONT86_000838"
     CDS             complement(19111..19239)
                     /locus_tag="ONT86_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR2351653.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(19242..20615)
                     /gene="rlmD"
                     /locus_tag="ONT86_000839"
     CDS             complement(19242..20615)
                     /gene="rlmD"
                     /locus_tag="ONT86_000839"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR2351654.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(20726..21781)
                     /locus_tag="ONT86_000840"
     CDS             complement(20726..21781)
                     /locus_tag="ONT86_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR2351655.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(21791..23221)
                     /gene="gatB"
                     /locus_tag="ONT86_000841"
     CDS             complement(21791..23221)
                     /gene="gatB"
                     /locus_tag="ONT86_000841"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCR2351656.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(23218..24690)
                     /gene="gatA"
                     /locus_tag="ONT86_000842"
     CDS             complement(23218..24690)
                     /gene="gatA"
                     /locus_tag="ONT86_000842"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCR2351657.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(24690..24995)
                     /gene="gatC"
                     /locus_tag="ONT86_000843"
     CDS             complement(24690..24995)
                     /gene="gatC"
                     /locus_tag="ONT86_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCR2351658.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(25101..27137)
                     /gene="ligA"
                     /locus_tag="ONT86_000844"
     CDS             complement(25101..27137)
                     /gene="ligA"
                     /locus_tag="ONT86_000844"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR2351659.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(27154..29388)
                     /gene="pcrA"
                     /locus_tag="ONT86_000845"
     CDS             complement(27154..29388)
                     /gene="pcrA"
                     /locus_tag="ONT86_000845"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCR2351660.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            29543..31102
                     /locus_tag="ONT86_000846"
     CDS             29543..31102
                     /locus_tag="ONT86_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCR2351661.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(31213..33144)
                     /locus_tag="ONT86_000847"
     CDS             complement(31213..33144)
                     /locus_tag="ONT86_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2351662.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(33162..34076)
                     /gene="pfkB"
                     /locus_tag="ONT86_000848"
     CDS             complement(33162..34076)
                     /gene="pfkB"
                     /locus_tag="ONT86_000848"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR2351663.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(34073..34825)
                     /locus_tag="ONT86_000849"
     CDS             complement(34073..34825)
                     /locus_tag="ONT86_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2351664.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(34984..35193)
                     /locus_tag="ONT86_000850"
     CDS             complement(34984..35193)
                     /locus_tag="ONT86_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351665.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(35204..36532)
                     /locus_tag="ONT86_000851"
     CDS             complement(35204..36532)
                     /locus_tag="ONT86_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351666.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            36668..36823
                     /locus_tag="ONT86_000852"
     CDS             36668..36823
                     /locus_tag="ONT86_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351667.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(36897..37484)
                     /locus_tag="ONT86_000853"
     CDS             complement(36897..37484)
                     /locus_tag="ONT86_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR2351668.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(37567..38817)
                     /gene="clpX"
                     /locus_tag="ONT86_000854"
     CDS             complement(37567..38817)
                     /gene="clpX"
                     /locus_tag="ONT86_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCR2351669.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(39064..40353)
                     /locus_tag="ONT86_000855"
     CDS             complement(39064..40353)
                     /locus_tag="ONT86_000855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR2351670.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(40507..41439)
                     /locus_tag="ONT86_000856"
     CDS             complement(40507..41439)
                     /locus_tag="ONT86_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351671.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(41596..42255)
                     /locus_tag="ONT86_000857"
     CDS             complement(41596..42255)
                     /locus_tag="ONT86_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR2351672.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(42258..43283)
                     /gene="sppA"
                     /locus_tag="ONT86_000858"
     CDS             complement(42258..43283)
                     /gene="sppA"
                     /locus_tag="ONT86_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR2351673.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(43296..43586)
                     /locus_tag="ONT86_000859"
     CDS             complement(43296..43586)
                     /locus_tag="ONT86_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351674.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            43995..45800
                     /gene="glmS"
                     /locus_tag="ONT86_000860"
     CDS             43995..45800
                     /gene="glmS"
                     /locus_tag="ONT86_000860"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR2351675.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(45872..47227)
                     /locus_tag="ONT86_000861"
     CDS             complement(45872..47227)
                     /locus_tag="ONT86_000861"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR2351676.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(47251..48420)
                     /locus_tag="ONT86_000862"
     CDS             complement(47251..48420)
                     /locus_tag="ONT86_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351677.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(48417..49304)
                     /locus_tag="ONT86_000863"
     CDS             complement(48417..49304)
                     /locus_tag="ONT86_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCR2351678.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(49493..53185)
                     /gene="nifJ"
                     /locus_tag="ONT86_000864"
     CDS             complement(49493..53185)
                     /gene="nifJ"
                     /locus_tag="ONT86_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR2351679.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            53384..53905
                     /locus_tag="ONT86_000865"
     CDS             53384..53905
                     /locus_tag="ONT86_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR2351680.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(54604..55161)
                     /locus_tag="ONT86_000866"
     CDS             complement(54604..55161)
                     /locus_tag="ONT86_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351681.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(55340..55951)
                     /locus_tag="ONT86_000867"
     CDS             complement(55340..55951)
                     /locus_tag="ONT86_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351682.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(56064..56321)
                     /locus_tag="ONT86_000868"
     CDS             complement(56064..56321)
                     /locus_tag="ONT86_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351683.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(56918..57196)
                     /locus_tag="ONT86_000869"
     CDS             complement(56918..57196)
                     /locus_tag="ONT86_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351684.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(57230..57481)
                     /locus_tag="ONT86_000870"
     CDS             complement(57230..57481)
                     /locus_tag="ONT86_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351685.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        15973 a        12511 c        10308 g        19162 t
ORIGIN      
        1 tggtgaggtt tctttaaaat aaaaatatcc ttgcattttc tcctagaaat aagtattttt
       61 aaattacaag actattctta aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga
      121 tgatgaacag acgattttag atggaatgaa acagttgatc aattggtctg agcacgggtt
      181 tcaattaata gggacttttt ccagtccgct gaaagcgctt gatttttctt tggtacatgc
      241 aatcgatatc gtggtcacag atgtaatgat gcctgaatta aatggaatcg aattatcgcg
      301 gctattaaaa cagcaacaac ctgaaacaca gatccttatc ctctctagct atgatgaatt
      361 tgatatggtt aaagattctt ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt
      421 gagtcctgat ttttttctaa attccttaac tgccgtttca caaaaaatca aaaaaaagcc
      481 caacaaaagc ccaatcacca atcgttatga gcagatctgc gagaatcttt cacagcagat
      541 tgctggacaa actcgtccat tgcttgaccc tcttcttttt gaacacacct tgtttttctg
      601 ttatataccg aaaaacggcc attcagttca aaacaaggct ctaaacaaaa acagtcgatc
      661 acccattcat tgatggatag cgacgattcg tttactattt atcctgtttc taccttttcg
      721 gaagaatccg gctatgtaat caatactgat tccaaagata cacttaggaa gtttgttcaa
      781 aagtttcgtc aagaagtttt tccaggtgat ttttttgttg tttctgattc ttttgcaatc
      841 caagatatca atgaaatatt ccaagagatc agaaattgca ccaaaggaca acgtttctat
      901 cataagggaa agaaccttgc ttatcagcat gaacttcttc cgttattaac caaccacgag
      961 cagcaagcaa gtagttattt aacaaaaatc gttcataaag actacgcttt agctgtcaaa
     1021 gaacttgaga atttcttcac gacgatattg gaacagccta ttgatccagc ttatttgaaa
     1081 catgaggcaa tcaatcgttt ctatgcatta ctcaacgctt tggctgacga gcatgttggg
     1141 aatgaagaac tttctttgct caagatcgcc cttccttctt tattagccga tgctgagtat
     1201 ttcgaagatt tttctattct tgtcaaagaa acacttgaga aactcaccca cttttctgca
     1261 aatgcaaaaa aagacaattt cgatatcttg actttgatct acgagttcgt ccaggaaaat
     1321 tatgagaaag aaatcaattt acaactttta tccgaaaaat atcatttttc atatagctat
     1381 ctctcagcca tctttactga aaaatttggt atcaactttt caaaatatct aaaaaaagtc
     1441 cgtatcagta aagccaagct ctttttgacg gaaacacggc ttactttgac ggaaatctgt
     1501 gagaaagtcg gctataccga actgggttat ttctccagag tttttaaaga agagacagga
     1561 atgacacctt ctcaataccg taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc
     1621 atgaactctt tgccaaactt ctgttcattt tgttcatcgg tctttttctg caagccatcg
     1681 tcatttcttt gtttatttat catcgatcaa gggatgccta tatccagctg ttcaatcaat
     1741 cgaatgatgt ggtactaaaa aagattcaat ctgaatttga aagtttgaat gatacaatcg
     1801 agaatacatt agccgctttt gattctaatc cagctgtcaa aagctatttt tccaatgatc
     1861 cagcccaaca tatggaacag cttcagcaac taagaacaat ccaaaaaatg aatgactctc
     1921 tttcaaaaat ccatccaatg atcgattacg atgtattgat ctttggagaa aatggaagaa
     1981 cttttgtcgg aaacgacatg ctcactgccg tatctgccga ttcttttttt caatctgcga
     2041 ttgctcagag agtgaatgaa cgtgctgctg atacacaaat gctttttgca caccacggat
     2101 tgactcttcg cgataagaaa gcacccagcg tcttctttgt caaaaaactg aaaaatacat
     2161 tgaatcacgt ttacggatat gctgttttat cgatctcatc caagcaacta gcgaatcttt
     2221 ttcaatctgt agtaaatcct gagatatcga agatctcctt catgaacgaa gaacagacga
     2281 tcatcgcatc aaatgaagaa aatacaattg ggagccgatc aatgattttc aaaaaactaa
     2341 cagtaggtga aacaacgatt tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc
     2401 aggattgtac attactcagt caagtcgatc ttcattcttt atctaaccaa atgggcgtga
     2461 tttttccgat tattttgtat aatatcgtga cacttgcttt tgctggctca ttggtattta
     2521 tgtatttgga tcgacacaca aaatcgatct accgcttgat tgattcgcta aaacggctag
     2581 aggaagatgg cttaaaagct actgttcctg tacaaggaag ccatgaagtc caattgctag
     2641 ggaatacgat caatcaattg ctaagtaatg ctcagcatca ttatgaacag acgatccaag
     2701 acgaaaagaa gaaacgttcg cttgaaatcc aaactatgca agcacagata cagccccatt
     2761 ttatttataa tacgctgact agcttgaaat ttttgatttg gcagaaaaaa acagatgaag
     2821 cagttcaagg aatcgacagt ttcattactt tactaagaag tacgattggg aaaaaagaag
     2881 aagtgattcc agtcaaggaa gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta
     2941 tgcgatatgg cgaaagaatc tcggctaaaa taatgattcc tgatgaactg cagtcattat
     3001 ctatgccgaa tatgatcctt caaccaatta tcgaaaacgc ctatcttcat gcttttcaca
     3061 ataaaacgca aggattcatc acggtgtacg gcaaaacaaa aaatcagctt cttttattcg
     3121 aagtgattga taacggcgat ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt
     3181 tttcaggaat cggcatcagc catgtagatg agcggatacg cctaatgtat ggcgatgcat
     3241 atggcttaac gatccaatca gtgattggga caggaacgac tgtaacgatt tgtctaccgc
     3301 ttcttcctaa aaatcaatca gaatatcaaa aataaagaat agccaagcgc tgattttttt
     3361 attttaagtt aaataaatca atcagtattc ttgactttca tctacgcgtc tcttctactg
     3421 tcagcactgg ataacagccc aatatttgac tttctcctcc gagcatcttg atttcttgga
     3481 gcgcattctc tactaatgtc attggacggt ccaacaacaa atcgatcacg aaaaaatatt
     3541 ctcccaagct agttttcaat ggtcgggatt caatcttact taagttgatt tcccgccagc
     3601 caaaagctgc cagcatttta tgaagcatcc ctggccgatt agcaggtagt gtcaagatga
     3661 cactcatttt tactggagct gactgctggc tggtcatctt tcggtcaccg acgatccaaa
     3721 aacgtgtctg gttcaactca ttatcttgga tattctcagc taagatctcc aatccaacat
     3781 gctgtgctgt ttctaatgaa gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat
     3841 acattgctgc agcagttgtt gattctgttg ctactaaagg aacattagga taatgcgttt
     3901 ctaaaaactg ttgtgattgt gctagtgctt gtggatgtga aaggattttt gtaatctttg
     3961 ttgctggatt gcctaaaagc tgctgcttga taggaagaac gatttcactt ttaacttcga
     4021 cttctggatg tttggaaaga agatcgattg tgtgatgaac agctccttcc aatgaattct
     4081 ctacaggaac gacagccaga tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga
     4141 tggaagcata cgcagtcaat tgttctgtag gaaatagctg acaagccgcc tgataagtga
     4201 atgaactttc tggccctaga tagctgacgt tcatatgctt ttgatccttt ctataatggt
     4261 atcgatgatc ttttctggtg tttgattggt cgtgttgatt cgatgtgtcg cactttcttc
     4321 ataccaagaa agtcgttgtg ccaataatgc ttttatttct tctcttgttt ttccaagagc
     4381 taaaggtcga atagacgttt catcttgttg gatacgatca actagacagt ccgcttctgc
     4441 ctctaagtaa agaacaatcg gctgtgtttt taaaaaacgc cgattttctg cactttggac
     4501 aatccctcca ccagtcgcga taattttgtc ttcatttgac agctttctta aaatatctga
     4561 ctccactttt cgaaagcttt tttcaccgta atagtgaaaa tattcggcaa tcgacaattg
     4621 gatatgttcc tcaatctttg tatctaaatc tacatactct ttttgtaaac attgagctaa
     4681 ccctttaccg attgttgttt ttccagcgcc catgaaacca atcagcaaaa tgctagccat
     4741 gagcttctcc tcctgacaca agttctgcta aatcgtcaaa aaatgccgga taagagattg
     4801 aaacagcttc agctccttct agttcgcaag gtccatcagc tatcaaagca gctacttgca
     4861 gcatcatacc gattcgatga tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg
     4921 gcccatggat gatcagaccg tctgctgtcg cttttatctt tgctcccatt ttctgtaatt
     4981 cttctgccac cgcatcaatt ctgttggttt cttttacttt taattcttct gcgtctttga
     5041 tgatcgtgat tccttctgct tgagtagcca ctaaagctag aatgggcaat tcatcaatca
     5101 gacgcggaat gatttctcca ccaatcgttg ctttcttcaa atgactcgtc ttgacactta
     5161 aatcagccga ttggttctgt tcattgtgat tcgtctgggt aatcgctgcg cccatctcct
     5221 ccaacacatc taagatacca gttcttgttg gattgacccc gacatttttt aacaacagtt
     5281 ggctttccgg aacaagaagt cctgctgcta gaaagaaagc agctgatgaa atatcacctg
     5341 gaacagttat ctgttgacca gttaattttt gcggtccggt cactatgatc gttttatctt
     5401 ccactgtgat ccttccgcca aattgacgaa tcatttcttc cgtatggtta cgggaacgtt
     5461 ctttttcaac gattacggat gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt
     5521 tgacttgcgc actggctaca ggcatattgt atcggatagg tgataaagag cgggttcctg
     5581 tcacagtgat tggtagatac tggtcatttt cttctccttg caagtcagcg cccatctcgc
     5641 gtaaaggttc catcactcgc cccattggac gtttagacaa cgaagcatcc ccgaacagag
     5701 tagtcgtaaa aggctgtcca gccaaaatac ccatcaataa acgtatggtc gtaccggagt
     5761 ttcccatgtc aattgattcg gttggttgga caaatgagtg gctaccgcga ccatgaatct
     5821 tgatcacctc ttcctcttct tcgatctcga cacctaattg ttggaaggca tgtaaagtac
     5881 tcagacaatc ttctgcgcgc aaaaaatttt gtatagtcgt cgttccttca gcaattgctc
     5941 caaacatgat gctgcgatgg gaaattgatt tatctgctgg tatcctaaca gtccctctta
     6001 atccatgtat ttgttgtaat aattgcattt atccagcctc ctatttgatc cagcaatgat
     6061 aactggtatt agacataatg attctttttg cttgtaacaa atcctgttgg tttttaaaac
     6121 tgatttgcag tattcctaaa atatcttccc tggtctcttg gatcttcaaa ttaatcaaag
     6181 aaactcccgc ttttccgatc agtccagtta cttctgcaat aacgccaggt tcatctggca
     6241 catcgataaa taaatcaaaa aaggcaggaa tcgcgccttg cttatggaca ggcaactggt
     6301 ctcgactttc ttttgcccga ttgaaaaaag aaaagatttc cgattggttc tcagtcatga
     6361 tccattctgc tatttgtgtc atttcttgtt gccaatctgc caatcgttcc aataatatct
     6421 gcttattgct caacaaaata tctgtccaca tgacaggatc tgaggaagcg atcctagtga
     6481 tatctcgaaa gcctcctgct gccagttgtt tggctcgcgg atactcttga ttgaattgtt
     6541 ctgcctgatt gactaagcca gctgccagaa tatgtggaaa atgacttagc attcctgtta
     6601 tacggtcatg ttctcttgct gtaagagtta catattttgc tttggttccg caaaatagtt
     6661 cttgcagttt ttcaacatct tttttcgtct tttccaaagg tgtaaagatg taataggcat
     6721 tttcaaatag tttagcatct gctgcctgta tacctgattt atgagaacca gccattggat
     6781 gtccaccgac aaaccgaaga tttttctgct cagcggatgt cacgacttgc tgcttcgtac
     6841 tgctgacatc cgtaatgatg acgtgatcag acaagttgat tctttcaagg acatcaagat
     6901 aagctaaggt tgtacggacg ggaacggcta ataagataac gtctgcttgt tctgcacctg
     6961 tttcaaaatc acttgcaacg taatcaacga tctgctgttc tactgcttgc tcacgggttt
     7021 ttgactgact atcccaacca actactatca gttctggatt ggtctgacga atacaaagtg
     7081 ccatagaagc accaatcagt ccaagcccca cgataaaaac atgttgtgcc atcttatttg
     7141 cccccttttt agtaatgttt caagtagtca cggtactcct tcactgcagc tttcaactca
     7201 acaagcgaat cacttgggaa ttttgacaat agctctgtcg ctaccacagt ggctacaaca
     7261 ttttccgcaa cgacgctagc tgcaggaact gctgtactat ccgaacgttc tacacttgct
     7321 ttgtaaggtt cttttgtatc gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg
     7381 ggtttcatga ccccgcgaat gacaagtggt tcgccattgg tcattccgcc ttcgaagccg
     7441 cctagattat tggtacgtcg gaaatagcct ctttcttctt cccataggat ctcatccatc
     7501 acttctgagc ctggtaatga accagaagtg aatccgaccc caaattctac gcctttaaag
     7561 gcattgatac taaccacagc ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg
     7621 tagcttccta atccagctgg aacgcctcca acgagcactt ctacaattcc tccgatcgta
     7681 tctccatttt tctttgtctg atcgatcaaa tcttttattc tttgctcaac tgctggatca
     7741 acaactcgta cttctgacgc ttcagaaatt tctttgattt cttgaatcga gatatttcca
     7801 ggtatttctg cttggattcc accaagacaa atcacgtgtc ctgctacgat gatgtcaagt
     7861 tcttccagaa ttttcttggc aattgcgccg attgctacac gcatggtcgt ctctcttgca
     7921 gaggaccgtt caagcacatt ccgaagatct cgatgatggt atttcatacc gcctaccaaa
     7981 tccgcgtgtc ctggacgcgg acgtgcaact cgccgcagtg attggtcttt ttcaggcact
     8041 tctgctacag acatgacagt tgtccaattt ttccaatctt tattttctac cactaaagtg
     8101 acaggtgaac caattgtttt gccgtgacgg atgccagacg tgatccgaac acgatctttt
     8161 tcgatcttca tccttcctcc tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg
     8221 atttcttctt ctgacagagg taatcctgct ggtagtcctt caataatggc tgttaattct
     8281 ggtccatggg attctcccgc agtcatataa cgcatcttac agtcctccta agtaatcttt
     8341 gaatgtctct gtgggaacag gaacgatttt tgcttgccca atcttttcta gcaagatgat
     8401 attcaattgg cttcctcttg ctttcttatc atgggttatc gcgtggaata attcatctgg
     8461 atgacttaga tcgtacgtaa tcggcagatg gaatttttga agtatccttt ctaaagcagc
     8521 tgtcgatcca gttggagaca agccgatttt ttctgcatga gaagtgatgg ctaccattcc
     8581 aatagctact ccttctccat gactgactac cccatatcct gctgtatttt caattgcatg
     8641 gccaattgta tgaccaaaat ttaaaataag tcgatttcct tggtcaaaga catcttcttc
     8701 cactactttt cgtttgattt ccaacgtagc agtaatgatt ggaacagcat gttccagaag
     8761 atctttttcg tctttcagct cgttcagctg tttccaaagc gttgcatctg ctatagctgc
     8821 tgatttgatg atttcagcga ttccttctcg taaacgacgt accggcaaag tttttagaac
     8881 ctcaggatca atcaatacac cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt
     8941 gtttgtattg actgctgtct tcccgccaat actagaatct acttgtgcaa gtagcgtagt
     9001 cggaatttga acgaaatgga tcccacgcat gaacgtcgag gccacgaatg ctgctagatc
     9061 acccaccatt cctccaccaa gtgcaagtat cccatcgctg cgggtaaaat cattatctgc
     9121 taaaaaatcg tataactcag ctgcttgttc taaagattta ctggtctcac ccgccggtac
     9181 gattgaaagg actggttcaa aattagcttg tctcaattgg aacatgattt tttctgcata
     9241 tagtggagca acgtttgtat cagtgatcac agctactttt tgaggtctcc aaatcgtttg
     9301 aagccatgat ccagcctgtt ctagtgcgcc tttttcaata tgaatatcgt atttttggtt
     9361 atcaacatgt acagtaagca ttcgtttcac ttctttcttt taaaaagctg gaattaatcg
     9421 agtgtctgaa tcttttccat tgcttctttc atgatcgcaa cttctttcat catgcgtgag
     9481 taagtctttt cattcaacga ttggggaccg tctgaccaag catttgcagg atcaggatgg
     9541 atctctacga tcatgccatc tgctccgctt gccacacccg cacgagccat tggtggtaca
     9601 agttcccaaa ttcctgtacc atgacttgga tcaacaatca cagggaaatg agttaatttt
     9661 ttcatcaatg ggacagcact taaatcaaac gtattacgtg tcgctgtttc gtaagtccga
     9721 atcccacgtt cgataaaaat gacaggagat ttgtcttcga ctgcaatgta ttctgcagca
     9781 ttaagccatt cattgatcgt accggaaatt ccacgtttca atccaacagg tttacctgtt
     9841 ttaccgacag cagatagcag tttaaaattt tgcatattac gtgcgccaat ctgtaggata
     9901 tcgctgtact cagccaccat atcgatatgc ccttcatcca tcacttcagt aatcactttc
     9961 attccaaatt catcagccgc ttgacggatg tatttcaatc cttcttcttc taaaccttgg
    10021 aaagcatatg gagaagtacg tggtttgaag gcaccacctc gcagaatttt tgcgcccccg
    10081 gctttggcca ttcgcgcaca ttctctgatt tggtctaatc cttcgataga acaaggacca
    10141 gccatcgtca cgaaactgcc gtcaccaatt tttactccgt ctacatccac gactgtattg
    10201 ccaggatgga attctcgaga agtcaatttg tatgtgttta aaatacgcac agcattttct
    10261 actccttcat agcgtgtaaa tgctccttct tgtaaacttc gcgtatcacc ttttagtcca
    10321 atcactactc gttctgttcc ttgatcgatt gagactttga atccttcttt ttcgatatga
    10381 tcgacgactt tttttatttg ttcttccgtt gcttcttttt tcataattac tatcattgtt
    10441 tttcccgctt tcttttgttt acttatctat cttggctgat tttctctact ttttcaaggt
    10501 ggttttacat taattcctac taaatgaccg tgtcctgttg ttctcatttt cgctccttct
    10561 ttcctactta tgacccagat aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta
    10621 cagtgaacat cttcaacgag ccttaaaaag cgctgcaaaa atataaaata gccactatag
    10681 atgaatttct ctacgtggct gtaagtcgtg tttaaaaatc tttagccatc atagatacaa
    10741 acctggtttg tttgtaccca cgaagtcatg aatacaaacc tatctaaaat agctaaagta
    10801 atacgtattc agttttgtaa gttttctaga tttcataaaa aatcgctcca aactgatgta
    10861 agaattgtac ctagtataga cataaaaaaa tcgactgtca atcattttta tgaaactttt
    10921 tttaattttc tgatagttaa ctttgttttt cagaaaaata acaattgatt ttattgaaga
    10981 aaatagtcat gaacaaaagt atctgagaac ttttttactc tcttgttcat gacatataga
    11041 ataaagcagc tttgtcttgt aaactccgtt tttcaaataa taatctaaag attgctactg
    11101 aataataaag ccgatttcat ggtcctcttc gctgctgatt cggtaaggtg actcaacatc
    11161 cgctccccag ctattgatac ccccgactcc tcttactttt gcaaaaatcg taagaaccgt
    11221 tcgtctttct ggcggtagtt cttctatatg tgttgcctgt tccagttctt ctgctttgaa
    11281 tggtaaagcg gaaaaagcaa atggcgtatc ctgctgttca aatcgcaacg aaaaatcttc
    11341 ttgatcttta tccgtattat ccaacgtttg atttcttgtt agttctaccc atttcgtttc
    11401 catatgcatg ccgcattctt ggggaacaag gtatggggaa acggggagtc ctgccacttt
    11461 ataaatacct tcgacaccac cagccatccg atccggatat gtttcccctg ataggccttc
    11521 atactcaaaa aattttgctt tcgttggcat aatgaacctt aaacccaatg cgggtaattc
    11581 cggtaatcct ttttgtccat aataatgcat attgacaagc attttaccct ctccagcgat
    11641 ttggtaggaa attgtcactt gggtttttgg tactgtgttg atttccagtg taaaaatgat
    11701 ttcaaccgtt tcagcgtatt cattacttga atattgatta tttactggag caatcggcaa
    11761 atcgatcttt ttattgtcca ccagtacttc tatttctttg acttttggat acaatccggc
    11821 tcctaaccac ataccggatt taaatccaaa tccgctacct ctgtcattat cggtcgttgc
    11881 tcgccaaaaa actggaagtg tttcccggta tagccattct tttccgtttt tattgagcga
    11941 ttccagtccg cctcttgtat aagaaaaaat ataatgcgta ttccccgatt tgacacctaa
    12001 tgtacagtcg ccgaagacga ttgcaacttt ttccgtatcc atatgacttt tatttactgt
    12061 atttttcata ataatatctt acctcctgca tggcgggttt ttcttgtcca tctgcaaaaa
    12121 cgatcccatt ccctgagaat tcatagtcac ttggtttatc gtcaaagtca ccgccatagc
    12181 gcatcacttc tctgcctgtg attggatcag tcacttggat cgcctgatct ttaaaatccc
    12241 atatatagcc tccttggtac atttcataac gatcaagcaa cgtcatgtaa gaatccattc
    12301 cacctaacga attccccatg tcatgcatat attcacataa gatgaacggt ttttccgctt
    12361 tattttcaag gtagtcaatg atctcttctg gtggcgcata catgcagctt tttacatctg
    12421 aaatcgaatc ttcgaatgct ggatttggaa aattcccttc ataatggaca agtcgatcgg
    12481 ggtctacttt tttgaagtaa tcgttcattt gtcgaatgtt ttcttccgca taagattcat
    12541 ttccaagaga ccaaaaaaga atacttggat ggttttttaa tgtttcgaaa ttcgtttttg
    12601 ctcgatcaag aacagccgcc tgccactgag gtactgcacc tggtacgttg taagaaggct
    12661 caaatgcgcc cattttttgc catgagccat gggattcgag gttcgtttcc gccatcacat
    12721 ggattcctgc ttggtcgcat aaataatacc aagtgatctg gttggggtaa tgagatgtcc
    12781 gtacgccgtt gatctttgca ttttttatga tagccaaatc tttttccata tcttctatag
    12841 tgatggcgcg tccttttttc ggatgccact catgtcgatt taccccgttg ataatcatcc
    12901 gttttccatt gaagtagatg attttatctt tgatttcgat tttacgaaaa ccgaaggaat
    12961 agtcaatata ctccgttact cttttttctt cgtctatcgt ctccaattca agcttatata
    13021 aattgggctg aaaatgcccc cacagtgcca cattttcaaa tccatcgatg cgaatcgtca
    13081 tttgctcttt taacggatat tgctgttgat agatttcctt cccatcagga tctagtatcg
    13141 ctacattcag ctgactgttt ttggctactt gctcaaaaga caaatctact tcaagtttgc
    13201 cattttgaca tttgtcatct aagcgaggtt tgatccaaag atcttctaaa tggttttgtc
    13261 ctcgagcaac taagaagaca tctctgaaga tcccaaaaaa tcggaaaaag tcctgatctt
    13321 ccaagaatgc agctgtactt cttttgtaaa cctccacagc tagaatattt tcttcttcca
    13381 aataaggtgt taaatcaaat tcagacggag tgaacgaatc ttcggcatac ccgataaaat
    13441 gaccattcaa ccaaataaac attgcttgtt ctacaccagc aaaatgaatc gagatttgtt
    13501 ttccctttag accttcttct aatcgaaaag tcgtcaaata cgaaccaacc ggattgtctt
    13561 tagcagctga aaagatccct tccgcctctt tctcagggct tgtcgtgaaa gggggacggc
    13621 gaaatacatg tccctcccac ggatacatcg tattgatgta atggatcttg tcataatttg
    13681 ctaattcaat atgactagga acagtgattt catcaaaaga acaggcatca aaatcttttt
    13741 tataaaatgt ttttggtcgt accgaaggat ttggcgaata acaaaaacgc catgatccat
    13801 ttaaagactg tttaagggag gatgtggttt ctacttcttc ccttgttgca taacaaacat
    13861 gatcgctatg tgcatttgat tggttcactc taaaaacttg tggatcatct aaccatttta
    13921 tatttgcttt catacttttt tccttctctc tcataacttt ttgaaatgaa gcagttctgt
    13981 ttggaaatca ccttgaaaat cggggaagta aagtccaact gtcatcaatt cggcccccga
    14041 aaatatccgc ttcgaggtct gttcctgata aagtgcctca tcatctaacc ccatcagata
    14101 tacttcatgg aacgctggtt gagctgaagc tagtatcctc cctaaaaaca caatagcttc
    14161 atcttgttta ggagacacaa acatccatgc ggcaatattt tcttcaaaag gactcgccaa
    14221 acgataataa tggccatact ggatcaccgg acgaatcgtt tgataggata taatttggtc
    14281 ttttacttgc tttttttctt caggagacag ctttgtcaaa tccagttcat aaccaaaaac
    14341 agcgctcata gctgtttctc ctcttgtttt caacgaagta gatctgtcgg tctgatgatt
    14401 tggtacagct gatacatgag ctgtgatgga cgaaatcgga taagcaaggc ttgttccata
    14461 ttggattttc atacgctcca ctgcatccgt gttatcactt gtccatgact gcggcatata
    14521 ataaataaac ccggcatcaa accgtccacc tccaccagaa cagccttccc ataagatttc
    14581 cggataatca gttgtcaaat gttcaagtag ttcataaagc ccgagcatat aacgatgcgc
    14641 tacttctcct tgtctctgtg ggtcaagggt aatcgaatag acatctgaaa gcgaacggtt
    14701 catatcccat ttgatgtagt caagaggaat cgtatctaaa atctttctca tttgtttctc
    14761 aatcgtctgc cgaacatcta accttgtaaa atccagtaca tgttgtgatc tggaagcaga
    14821 aggcattcta cctggctcct gcataagaaa atcaggatgt gttcgataca gttcagaatc
    14881 aaccgagatc atttcaggtt caaaccataa accaaatttc aatcctttgc tatgagcata
    14941 atctgctatt tcacgtaatc catttgtcaa cttgccttca tactcgaacc agtctcctaa
    15001 tgaagaattg tccgcatctc tttttccaaa ccagccatca tctaatacga acatttcgat
    15061 gcctagctcc tttgcttcat caatgatcgc cttgattttt tcactattga agtcaaagta
    15121 agtggcttcc cagttattga ccagaatcgg ccgttcagca aattgatgac gacctcttgc
    15181 gattctttca cgtagcaact catggtggat ctgactcatt ttgttcaatc cctgacttga
    15241 gtaagaaagg atcatttcag gtgtttgaaa agactggcct gcattcaatt cccacgaaaa
    15301 acgatggctg ttgatcccac cgacgactct caattgattg atctgatctt tttcaagttc
    15361 gaaagaatgg ttgccggaat aaacaaattg aagtccgatc gcttctccag tattttctgt
    15421 cgtatgatga tgaactaaag caataaagtt attcatatgg tgactgctcg ttcctcgacg
    15481 gctttcaaat acttttgttc cagataaaac agattgccgt gaaatctgtc tttcattgac
    15541 atgtgctcct ggcaaagcaa tgacttcatc aaatcgccta gtatgggcaa agtctagctg
    15601 gaaactagcc gctttttcta gtttgattgt ttctccggtt tcatttctta tctttacaga
    15661 tcgtgtgatc actgctctat cagtaaatat ggtatagaac aaatcgaaat aaatcgctgt
    15721 ttctctatcc ttaagtgaaa tcaccaacgt ttgtgcctct gacttgtcca tcacataagt
    15781 ttgaggcaag ccttcgatct ctggcttccc atctgtaata taatggctgt catatctcag
    15841 atccagtaag tctgatccat tcttgcgctg aatgatcgat gcgggcgttc gaaagtccat
    15901 gctgccatga gaagagtatt cttgcggtag tgtatctttc gacagagacc ggtcaggatt
    15961 taagggaaca tttcctgaaa atccacgatc tcttcgggga taatttttat tgtctttgta
    16021 ctgcttgact ggtttgccaa aataaatatg cgccaataca ttgctttctt ctatctttat
    16081 cagataagaa agaaatgtat ttcgtaaatg gaagactcgg ctcttttcat caaataagat
    16141 gaaattttta tctgtttttc tatgcgtaat atccatcatt tcactcccaa caaaacggaa
    16201 accgtttact tttttttata attcctgtat actgtttact aaacaatttt gtcaataaaa
    16261 attttttatt tttttgagga gtgtgtaaaa atggttggaa ttagagatgt cgcacgaaag
    16321 gcaaatgtct ctcctgctac agtttcccgt gtgttgaata acgatcagac gatccatgtg
    16381 gctcagacta ctcggcaacg gatagtagag gcagctgctg cgttggatta tgacatcagt
    16441 actcgtaaat atatcaaaaa gagaatgcct tcgattggtg tgatctctac gatcggcaaa
    16501 gcgagcgaag aacaggatat ctattacaaa catttgcgag tagggatgga agaagaagct
    16561 cgacgtctgc atttagggat gaatcgaatc tataatctat ctgataatcc taaacaatgg
    16621 gaagatctag atcagttagg tgcggtgatc gttgttggga ctgttacgaa agaatcgatt
    16681 accgccttgt taaaacaaaa catgcaaata gtcgtcgtgg ataaccctga catccagcaa
    16741 gaggtcgata tggtgtatgc agatcttgaa cggatgacta aaaccgtact ggaattgttc
    16801 ttagaaaatg gtcataaaag aatagcctat atcgggggat ataaagtcga tatggatgaa
    16861 ttcggtggga agcagatttc tataaatgaa aagaggatgc gtgcctatca gcattttatg
    16921 atcgatcatc acttagaaaa atatatgtct tatcatctag gtggctggac agaaaaacaa
    16981 ggtgaggtac ttgcgactaa tctattagaa aatgaatccc ctcttcctac tgccattctt
    17041 gtcgggagcg atccaatggc aattggtgtc tatcgggcat tgaaacaaaa aaatatccaa
    17101 attggagaag agattgttgt agcaagcttt gataatatcg agacaatcgc aaacctctcc
    17161 cctggcttga ccagtgttca aatcaatgcg aaatcaattg ggcaagcagc tgttcgaatg
    17221 gcattggagc gaattgacgg tatacgggac gaaccaatca ttttgactta tcctgcacga
    17281 ttgattgtta gggaaagttt tatcccaaga acaaaaaggt attgaaagag aggttgtgaa
    17341 aagccgcttc gtttcttcat actcaaaacg aaacggcttt ttcacaacct cttacttata
    17401 cttattcaca aaatccaatg tctgctggta acaattttgc gattctttta gcgtgtaatc
    17461 aaattggtat tcatgggtaa tctctttttt tgtatctgta taaaacaagg attgcacggg
    17521 tacctttaat tctgtcaacc gattttctaa tgcttgacct tgctcctgga atgagtatgc
    17581 atttccgtca gttatatacg ttggcggaaa ttccttagaa acatggtcag ccacactagc
    17641 ttctttcaat tgctcactcg ttttccaatc ttttgttcca atcaacgacc acgcaacggt
    17701 tttgacgaaa aacttcataa atcgatcatc cgaatgctgc tgtgctgtta gcttcaaatc
    17761 aacaggtccg caataagaaa tggttccttt cagactatct tttggaagta gctgtttgat
    17821 tttcatttgt ttagcatacg catcattcgt ttgtatagct gcatattgca atgcgatttg
    17881 tgaacctgca ctatctccac caaaaaataa ttgttccatg tctagcaatt tgtcgtcttt
    17941 ttcttcagct aataaatctt tgatcagttc attcacttga agaacttgat tagggtattc
    18001 tgaatcaggc gctaattcat aattcatcgc aaccactgcg atgttggcgt ctgaaactaa
    18061 ttttgtcgca aattctttga cacccgattt atctccgccg acgaatcctc caccatgtac
    18121 ccaaaataga acaggaacag gccccttgct gttttttgga tagtagatgt cataagtatt
    18181 ctctttatac ttggactcat acgtcaaatc tgtttttttc gccacgtttt tttctgcttg
    18241 atcgaacgtt tttttatcag tgatttgaac agtatttgaa aacatatggt tgatcaagaa
    18301 tgctccaggt ctgggggaaa gctggaaggc aagaaagatt gcaaacgcga tcccaagaat
    18361 gattccgaag aaccatccga taaatttaag aattttcttt ttcataaggc tccctcagta
    18421 gttagtatta agcattctga ttataccata gtgttatttc ctatcaagct ctcctagatg
    18481 atttaatgat tttccccaaa cttcttctgc aatgcttttg acaaaagaaa ttttttccat
    18541 tgttcttcaa acaaaacatt ttcttcttct gttgaagcaa atccacattg aggacttaag
    18601 cacaaacgat ccaaaggcag atatcgtgtc gcttcgtaga tccggtcaat cacttgctgg
    18661 tgctcttcca tttcgccatt tttcgttaca gtcaaaatct aagacaacta atttatcttc
    18721 agagactttc tctaaaagaa caaaactccc agcacgttca ctatcgtatt ctaaataata
    18781 ggcacggaca ttttcttgag taaaataatg ggtctgctac gtcgtcatcc cccctgatgc
    18841 cgccaaaatc gaataataat ttccgcgtca aacatgtata tttagtgttg aatcatttga
    18901 gaatatgaaa ataaaaatct ttttgatcaa tcagttaatc aaattgaaat aacgaaaaat
    18961 ctgagacaga atgacgttca ttctgaccca gatttgattt tgcacaactc aacagtcctt
    19021 attggcaaat cgccaaaata atgtagacta actaaaaaga ctaagaaaca ataacaaaag
    19081 ttaacacttc ttagtctttc tttagcacgt ctatttatta aagattttag ggccttttct
    19141 catgttttta cgatcattct taggtgtttc catttgtttg gatcggcctc caccttgttt
    19201 ctttttgatc aattctttca ttttttcttt ttggtccatt tttattctcc tttaacgaat
    19261 gaagcgacta attcgatatg atgggtttgc gggaaaagat ctacaggctg aactttcttc
    19321 acggcataac ctgcttctgc aaataactta gcatctcttg caaaagttgc cggattacat
    19381 gagatataga cgattcgttc tggtgacatt tcgattgctg attcaataaa atgatgatct
    19441 aatccttttc gtggagggtc aactacgatc acagatggtt tgatcccttt ttcttgccat
    19501 tttttcataa ctttttctgc ttttccgact tcataatgaa cattttcgat tttattattt
    19561 aaagcattga actgtgcatc ttcaattgct tctggaataa cttccatccc atacacctta
    19621 tccactctgt ctgctaaaga caagccaatc gtacctattc ctgaataagc gtcgatcaca
    19681 atgtcttttt tatctaactc ggcaaactcg atggctgttt ggtacagata ctctgcttgt
    19741 tgagtattta cttgataaaa tgactttgct gagattcgat aggtttttcc taatagctga
    19801 tcttcgatat agtctctgcc atataaaact ttttctttgt ctcctaaaat cacatttgtt
    19861 ttttcttcat tgatattttg gatgaccgaa accacttctg gcaattcttc atgaatagct
    19921 tcagcaattt ccttggattt aaagaatttt tctttacgag taactaaaac gaccatcatt
    19981 tcatgggaat aatgaccacg acggataatg atgtgacgaa gctgtccttc atttttttct
    20041 tcattatatc ctcgtacttg gaaccgttgc aagatatctc tgactacttt gattgcttga
    20101 tcgatgacag gatcttggat ataaaaatct tcgataggga ctagatcatg gctattcttt
    20161 ttgtaaaaac cggtggctaa ctgtccatcg atacggcgaa cagggatctg tgctttatta
    20221 cggtagccga atggttctgc cattccgacc gttggaagta caggaacttc tggcatctta
    20281 gcaatcttcg ccattacatg ctctacttgt ttttgtttga agacaagctg ttgttcatac
    20341 ttcaaatggg ctaaaggtgc gatacctatg cgaagtaatg cattgttttc tacaggctga
    20401 cgatcaggtg attgtgtaat aaatgtttcg acttttccat atccaaactt attgcctgct
    20461 tttactacta gtatttctac ttcttctcca gggagtgcat tttcgataaa taatggataa
    20521 ccatcgattt ttgctacccc cattccttca taactcaagt cgacgatttc gactgtatat
    20581 cgttcgtttt tctttactat ttgctgttct gtcatatttt gctcctttaa gcctattttt
    20641 caatcctact gaactctact ataaatcagc taattacaca actaaaaccg gcactgacct
    20701 tactacgttc agcaccggct tttgattaat tcttttgatt aggtgtttcg tctgctattt
    20761 ttccgtcacc gtcgatatct tcatccgtta aacgttcgac ttcttttacg aattctttgc
    20821 tgacaacctc taattcttct tcatctgtac ctgtgattgc ttcatcagga atctgatcgg
    20881 tattggcaaa gaactcaatg tgttgatgaa gattttggaa atgcatcggt gcttccccgc
    20941 cgtattcacc atctaagttg atcattactg gtcgattgat atcatcttct gtttcaacga
    21001 acaaactgct tgttttggta tagattattc ttggatcatc gacatgtcga ccgccattca
    21061 gcatgagtgc tgcaatatgc aagatctcaa agatatttgc tgtcttcaca atgattaaag
    21121 aaaacttccc atcatccaat ttggcatctg gcgcgatctg ttcgaatcct ccaacagagt
    21181 tcgtcagccc taggaaaaac atcgaagcat ttccgatata ttccccttca tcatacttca
    21241 agcgcatttt gattggcttt actcgaggga gcatctctgc tcctttcgca agataagcaa
    21301 gataaccaaa aatactcttt agttctgatg gaacttcata agtcaattct gtcaaatagc
    21361 cacctgcagc gatattgatg aagtaactgt tatcagaaag gccgatgtcc atttttacgg
    21421 tttggtgttt ttttatcact tctgctgctg ctttaatatt atctctagga atttttaatg
    21481 ctcgagcata gtcgttcgtt gtaccagctg ggatgatggc cattttagga cggtgttcaa
    21541 gcggagcgat tccattaacc acttcgttga ttgtgccatc gccaccagct gccactacta
    21601 gatcaaaacc tagttctgcg actctacatg cttcattctt ggctgaatct ggttctggtg
    21661 tagttgcata ggcgctggct tcaaatcctg cttcttctaa tgcctgtagg atatccgcca
    21721 aatttttttt caataattct tttcccgagg tggggttata aattacgcgt gcttttttca
    21781 ttttcagcct ctatcgtttc gctaattcat ctttaagtag cttattcacg acaccaggat
    21841 ttgcttgtcc tttagttgct ttcatgattt gaccgactag gaagccaacc gcacgatctt
    21901 ttccattttt gaaatcatcg acagattgtt ggttgttatc caaaatttca ttaatgattg
    21961 gcagtagttt gttcggatca gataattgca cccaaccatt tttttcaact acttcttggg
    22021 cttctccgcc atttgtgatc aattcacgga agactttttt ggcgattttt gagctgatcg
    22081 ttccatcggc gatcaaacga atcattcctg ctaagttctc aggtgtcaat tttgtctcag
    22141 ctaattccag tttctcactg ttcaagtaag cagaaacttc acccatcaac cagttagatg
    22201 cttgcttggc atctgcacca ttttccaaaa cagcttcaaa aaaatcagac atttcttttg
    22261 taagtgtcaa taccattgcg tcatactctg gaagtccaaa ctcacggata tagcgttcgc
    22321 gacgagaagc tggcatctca ggcaaactag cttttacttt ttgaatccat tcgtcatcga
    22381 tcacgaattt cgggatatca ggctctggga agtaacgata atcactagac ccttctttga
    22441 cacgcatcaa aactgtttta ttcgttgttt cgtcaaaacg gcgtgtttct tgttggattt
    22501 ctcctcctga aagaagaact tttgcttgac gtttttcttc aaaagctaaa ccttttttca
    22561 cgaaagataa agagttcaag tttttcaatt ctgctttcgt accgaactct tcttgtccat
    22621 aagggcgaag agaaatattg gcatcacagc gcatagagcc ttcttccatt ttcacatcgc
    22681 tgacttctgt aaattggata attgatcgca atgcttctag ataagcgtat gcttcttctg
    22741 gtgaacgcat atcagcttca gaaacgattt cgatcaaagg cgtgccttgt cggttcaaat
    22801 caacgtaaga ataaccgcca ataccatgca tgttttttcc agcatcttct tctaaatgga
    22861 cacgttcgat ccggattttt ttctttttac cttctacttc gatttcgatc catccgtcat
    22921 gaccgattgg ttgatcaaat tgggaaattt gatatgcttt tggattatct gggtaaaagt
    22981 aatttttccg gtcaaaatga gtatctttag agatttcaca gtttaatgcc aaagctgctt
    23041 tcatcccgaa ttccagcgca gctttattca ttactggcag cacgcctgga taaccccagt
    23101 cgatcacgtt tgtattgctg tttggttctg caccaaaatg agctggcgct ggagagaaaa
    23161 tttttgagtt ggtttttaac tcgacgtgga cttctagtcc aatgactgtt tcaaaattca
    23221 ttcgtctttc cctcctaaaa tagctggttg ttgtttatgg aattctgttg cttgttcaaa
    23281 cgcatatgcc gcttgataca tcgttttttc atcaaaataa ttaccgatga tctgcaatcc
    23341 aactggtaat ccttctgata aacctgccgg tacagacata cctggaagtc cagccaagtt
    23401 gacagggatc gttaagatgt cactcatgta cattgtgatt ggatcgttaa tgttctcacc
    23461 gattccgaat gcaactgttg gtgaagatgg cccgatgatc aagtcataat ctgcaaagac
    23521 tttttcaaaa tctcgtttga tcaatgtacg gacttgtcct gcttttttga agtaagcgtc
    23581 ataataaccc gcgcttaagg agaatgtacc taacatgata cggcgtttta cttcttcacc
    23641 gaatccttct gaacgtgagt tgacatatac atcatctaat gatttcacat tttcagagcg
    23701 ataaccgtag cggataccat cgaatcgttg caaattggaa cttgcttctg atgaggcaat
    23761 gatataataa acagccacac cgtattttga atgaggcaag ctgacttctt ccacaacagc
    23821 acctaatgaa cggaatgttt ctgctgcttt caatacagct tcttttacgc caggggccac
    23881 accttcgcct aagtattctt taggtaaagc aatcttcatt cctttgatgt cgcctgtcag
    23941 tccctctgca aaatcaggaa ctgaaacgcc tgaagaagta ccgtcttttt cgtcataacc
    24001 gctgatagct gtcaatgcta atgcattatc tttcaccgta cgggtcattg gtccgatttg
    24061 gtcaagagat gaagcaaagg caatcaaacc aaaacgtgag acacggccat aagtaggttt
    24121 catccctacg atgccgttaa aagctgctgg ttggcggata cttcccccag tatcactacc
    24181 taaagaaact ggaatttgtc cagctgcaac tgctgctgca gaaccaccag aagagccacc
    24241 aggtactttt gttgggtccc aagcattttt tgttttcttg aaataagaag tttctgtact
    24301 tccacccata gcaaattcat ccatgtttag cttaccaaca gggatcatat ctgcttgata
    24361 gattttatcc atgaccgttg catcataaat tggttcaaaa ttgtaaagga tcttagatgc
    24421 agcggttgtc aagatatctt ttgttacgat attatccttg ataccaattg gaataccagc
    24481 aaggacgttg ctttcatcaa tccctttttc atctaaagct tttgcctgtt ccaatgcttt
    24541 ttcttcgttc aatgtaatga atgcctcgac ctcaggctct gtttctttga ttcgttcaaa
    24601 tgttgttttt gtcaaatctt gtacagtgat ttctttcgat acaagcatat gatgcagttc
    24661 ttctagagaa cgattgtgta attctgtcat tatgcgccag cctccccatt atcgatgatt
    24721 gcgggtactt tgataaagcc gtcttctgtt tccggcacat ttttcaataa ttcatcgcgg
    24781 ttccatccag cttgcgcccg atcttgtcgc atcacgttta ctgtttcaac cacattcgac
    24841 gtaaatggaa caccttccgt atctacttct tccagcattt caaccatatc gatgatttta
    24901 cctaattggt cggtaaaact tgcaagttct tcatctgcga acgcaagttt tgctaatttg
    24961 gcaacgtgtt tcacttgttc ttcactaatc gccatactac cccttctttc taaacgagtt
    25021 aaaaactttg ctctctattt taacacaaga gtgccgtgtc tgaaattgtt tattgaaatt
    25081 tgtctgaaaa aactcatttt tcattcttct actgagcgac ttttagccaa tgcatctgcc
    25141 atttgatctt ctgtccaaac ttcgattcct aattcttgtg ctttggtcag cttgctgcca
    25201 gcatcttctc ctgctaccac gatatccgtt tttttcgata cgctaccagt cactttcccg
    25261 ccaagatttt cgatcgtctc ttttgcttct tcacgagtaa atcgtgttag tttaccagta
    25321 aggactactg ttttttcttt aaaaggagac tcgacttctt gcaattgggc atttcgaagc
    25381 cctttgtatt cgaaattgac gcctgcttgt tttaattcat tcatcaattc atggacttct
    25441 tcgttactga aataagtgac tacactgtcg gcaatgatat caccgatcgt atcaagcgca
    25501 ataatcgact catagtcact tttactcaat gtttctagat caccgaaatg ttctgctaaa
    25561 atttttgctg ctttcgctcc gacatgtcgg attcctaacc cgaaaatcaa ccgctcgacc
    25621 gaattatctt tgctgttgtc gattgctgtc aaaatattat ttgctgattt ttcttttatt
    25681 ttgtccaacg tcaataactc ttcttctgtc aatttgtaaa gatcagcaac atctgctact
    25741 agttttttat catacatctg ttctagtacg cgcggcccca acccatcgat attcatcgca
    25801 tttcttgaaa caaagtggtt caaaccttct ttcatttgag cagggcattt aggattgatg
    25861 caccgtaagg ctacttcttc atccaaatgg acaagttcac ttccacaaac tggacaatgt
    25921 gtaggcaatt ggtattcttc gctgtcttta ggtcgtttat ctaacacgac ttgcgaaact
    25981 tctgggatga tgtctcccgc tttataaatc agaactgtat ctttcaagcg gatatccttc
    26041 aacttaatat aatcaccgtt atgcaggctg gcacggctta ctgtcgttcc tgctactcgg
    26101 actggtgtca tgatagctgt tggcgttact acccctgttc gaccgactgt ccattcgatg
    26161 ttttcgatta gcgtttcaac ttcttctggc ggaaacttgt aagctgctgc ccatctcggt
    26221 gctttgatag taaaaccaag ttgatcttgt agcgagaatt cattcacttt gatgacgatt
    26281 ccatcgattt catagggcaa atccacacgt ttgtcatgat attcttcaat gtatgaccac
    26341 acttcatcaa tcgaatgaca tagacgtttt tcccgattcg tgtggaatcc aattttttct
    26401 agctcttcta gcgcatcata ctgggttttt gcttgcatag gaccgaaatc agctactgtg
    26461 tataaaaaag tgtccagatt tcttttcgct gtgattttag aatctaactg acgcaaagaa
    26521 ccagctgcgg cgttccgagg attcgcaaag atatcttttc cctctgcttc tctgtcttgg
    26581 ttcaattgga caaatgaccg ttttggcatg aaacattcgc ctcttacttc gatgttcatt
    26641 ggttctttta atttgattgg gaccgaccgg acagttttta ggttttcagt gatgttttct
    26701 cctacagttc catccccgcg agtcgcacct ctcacgaaat ttccatcttc ataacgcaaa
    26761 gaaacggaaa gtccatctat ttttagttca cagcaatagt caactacacg tcccactgct
    26821 ttttgtacac gttgatcaaa agctatcagc tcttctttgc tgaaaacgtc gttcaaactg
    26881 taaagcggaa tatcatgagt gactttttca aaaccttcta atacttttcc gcctactcgt
    26941 tgagtgggag aatcagacgt gatcaaatca ggatattgtt cctcgattgc tactaattct
    27001 gcgtattctt tatcataaac atagtcttcc actgttggtt tatcttctac gtagtattcg
    27061 cgagaccatt ggttcagtct tgtacgaagt tcttctgctc gtttagcagc ttcgtcaatc
    27121 gtcatttcat gttccatcat ttaccctcca ctactaagct ttttcaattg gcgcaaaggc
    27181 agccaaaaga cgttttactc cttgacttgg gaaagccaca tctagttcta gatccttcga
    27241 cgtcccgctt actcggacga ctgttcctag accccatttt ttgtgtttga ctttatctcc
    27301 aggtttccag ctgttatttt cccctccagt agcactttta gagggaacaa ccgctgtctc
    27361 tggctgttta tagctatatg ctgttttttt agcgaataca ggcgtttttt taggcgtagg
    27421 acgcatgcct tcgatttcca gtaactcctg atcgatttct tctacaaaac gagaaggtcg
    27481 gttatattgc gtccgtccat ataatgttct ggagaaagca tttgttaaat acaacgcttc
    27541 ttctgctcgc gtgatcccca catatgcgag acggcgttct tcctccagtt cgctttcttc
    27601 catcaatgct cgagataaag gaaaaacccc ctcttctaga ccaatcagga atacaactgg
    27661 aaattctaat cctttggctg catgcaaagt catcaatgtc acttgggaag catcttcttc
    27721 taaattgtca atgtcggaaa caagtgccag atcatttaag aagaccgtta gtttttcttc
    27781 tggtgcatca gcgtcttctt cattttgctg ttcgaattgt ttgtcgaatt cttgtgtgac
    27841 cgttaaaaat tcctccaagt tttcgatgcg tgcttgtgct tctaatgtat tttggatttt
    27901 taagtcttct agatatccgc tacgatccaa aacctctttc gtcaattctg tcactgtcaa
    27961 gtatgggatc atctgagtga cttcttggat catctctcca aatgcaccta attgctgtcc
    28021 tgcttttcca ccgatatttg ccagatccac attttgcgct gcttcaagca gtggccattc
    28081 atgaagagaa gcaaaactgc gtagtttttc gatagatcct ggtccgatgc ctcgtttggg
    28141 cgaattaacg atacgctcaa aactgattga atcttgagga tttaccagca cattcaaata
    28201 cgctaaaata tctttgattt ctttacgatc gtagaacttg tgccctccaa ccattttata
    28261 aggaatattt gctttcaaca gagtttcttc cattacacga gattgtgcgt tcgtccggta
    28321 caagatggca aagtcgccgt aattccgatg attgcttcga atctcttctt gcatccggtc
    28381 tacgataaaa cgggtttcgt ctcgttcatt atctgcacgg tagtaagtga tcttgtttcc
    28441 ttctttgttt tctgtccaca agttttttgg ttttcgattg ctattgtttt caatgacttg
    28501 gtttgctgcg ctaaggatgt ttttcgtgga gcgatagttt tgttctagca aaattactgc
    28561 agcatctgga taatcttttt caaaatccaa gatgttctgc atatctgcac cgcgccagcc
    28621 gtaaatactt tgatctgcat ctcccacgac gcacaggttc ctaaatctac ctgccaataa
    28681 attgactagc gtatactgtg cgtgatttgt atcttggtac tcatctacat gaatataatg
    28741 gaatttgttt tgataatacg tgagtgaatc aggatgttct tcaaataatc gaatcgtatt
    28801 catgatcagg tcgtcaaagt ccatacattg attatattga agctcttttt ggtaagcagc
    28861 ataacatttt gccgcaatct cttcgtaata actgccttgc atttttgcaa aatcctgagg
    28921 ggtctgcagg ctgttttttg cttgagagat tgttcctaat atacttcgag gatcatattt
    28981 tttcggatca atgttcagtt cttttagtat ccgtttcatc aaggtcagct gttcagaaga
    29041 atcgatgatt gtaaagttcc ggttataccc gataaaatca acatctcttc ttaaaatacg
    29101 cacacacatc gagtgaaaag tcgataccca gacatcttcg cctccagatg cgagaatcgc
    29161 attgacacgt tctttcattt cttttgcagc cttattggta aaagtaatcg ctaggatgtt
    29221 ccaaggattg acttcttttt cctcaatcaa ataggcaatg cggtgtgtca gcactctcgt
    29281 ctttccactt cctgcacctg ccattagcag taatggtccg tcagtgtgaa gaacagcttc
    29341 tttctgacgg gggttcatcc cgtttaataa ttcagctttg gatgacatta acaacatcct
    29401 ttcttttatc aatcttgtta attatagcat tttcttggct ttcattaaac tagctagttc
    29461 aaaatcattt gtttcttttt atggattttt ccttgtcaat tcgttgattt cgcgctaaat
    29521 taaaagaaag cgaggcgttt gaatggaaaa aaaaggaaaa atcaagaaat tagataagac
    29581 tcagatatat tttattctaa aaggaatatt tgttggactg ctgacaggaa tcgtcgttag
    29641 cctgtttcga ttatctatcg aaaagctaag cgatttagtt cgaagtatct atgaaatgag
    29701 tcgggaacag ccgatttact taattggcct agctatttgc tgcatccttg cagcattttt
    29761 cgtgggttat ctggtcaaaa atgaaccgga tatcaaggga agcgggattc cacaagtaga
    29821 gggacagctt cgcggggaat tatcaatgaa ctggttctct gttttatgga aaaagttcat
    29881 tggcggtgta ctttcagttg gtgcgggcct ttttctaggt agagaaggac cttctataca
    29941 gcttggcgca tccattggac aaggagctgg acaattgttt cggagtccat cttcagaaga
    30001 aaagatttta atttctagcg gtgcaagtag cggacttgca gcagcattca atgctcctat
    30061 cgcaggatta ctctttgttt tagaagaaat ccatcactcc ttttctccac ttgtttggct
    30121 gacatcattt gcttcagcaa tcactgctaa cttcgtctct ctttactttt tcggcttgag
    30181 acctgtttta tatctaggtg accttccttc tttgccgttg aaatattatg gttccttagt
    30241 gatcttagga gtacttctag gcatcttagg ttttatttat cagaatgtct tgctggcttt
    30301 gccaaaattt tacaaccgat tgcctttacc agcttatttt cacgggttcg ttcctttctt
    30361 gctgattttg ccaattggtt atttatggcc tagtactcta ggcggaggca atcaaatcgt
    30421 tttagctttt ggacatgctt ccttgccaat cattatgatt atcggtttgt ttgttcttcg
    30481 attcgttttt tccatgattt cttacggtgg gggtcttcca ggggggattt tccttccgat
    30541 attgacattg ggggctttaa tcggtagctt ttatggaaac attatggttg atttcgggat
    30601 ggatccaatc tacatcaagg attttgtcgt tattgccatg gctggttatt tcacagcgat
    30661 tggaaaagca ccgctgacag caattatttt agtgacagaa atggttggct ctcttaatca
    30721 tctgatgcct ttaggattgg ctgctcttgt tgcttatatc gtcaatgacc ttctcggcgg
    30781 taatccaatt tacgaatcat tattggaacg tctacttgga aatactcaga ccgcctcact
    30841 aaaaggtgca aggacaactt ttgattttcc agtaacggca gagagttctt tagacggatt
    30901 aatggtacga gacttcaatt ggcctaaaaa tatgctgcta atctctatac gcagaggaaa
    30961 tcaggaaatc ttgactcacg gtgatacgat catgaatgta ggagatattt tggttatctt
    31021 gactgatgaa gcaaacttgc caagaatcaa gcaggaaatc gaggctaaat cttctatcac
    31081 aaatttgaag caatcatttt aacgaacatg gaaaaactat tttacaacaa ctattcatct
    31141 acactacttg attctatatg attctataaa aaaagaagag gctgggaaaa cccagcctct
    31201 tcttttttat ttctatgctt ctgctactgc aggagctttt ctcaataaac cataaatcac
    31261 accagatacg actgctccga tagcaataaa caccaaatac atgaccgggt gactcagtaa
    31321 gaatacaacg aaaatcccgc cgtgaggagc cagtaatttg attccgaaag ctccgactaa
    31381 tccgccagtt aaagcagaac caataataaa acttggaatg gcacgaatcg ggtcagctgc
    31441 tgcaaatgga atcgatcctt ccgtgacaaa cgatagtccc atcacgatgt tcgtcaaacc
    31501 agcatcttga tctgttttgc tgaatttatt cttaaacaaa cgtgtagcaa cgaaaattgc
    31561 caatggaggt accattcctc cagccataac agaagccatc acgacacttc cgccagttgc
    31621 aacagtcgta gctaaagttg ctgtaccaaa cacataagcg gctttgttga ttggaccgcc
    31681 taaatctgcc gccatcattc cagctaataa tgcaccaagt aaagcagcat tcgaaccact
    31741 catacttgtc aagaaaccgt tcaatgcatc attgattgct ttcataggga cattgaccag
    31801 caacatcaag aaaccagtaa tcagtaagcc aaacactgga taaaagagga tcgttttaat
    31861 tccatccaat gattttggta agttcttgaa taatttacgt aaaaatacga tgacataacc
    31921 agctaagaat ccaccaatca gtgcacctaa gaatcctgca ccgcctgtat tagctaatgc
    31981 accagcagca aatccaacga tcaaaccagg gcggtcaccg atacttgaag caataaatcc
    32041 tgctaatacc ggcagcatga aaccaaatgc agcttgtccg atttggttga accagctagc
    32101 agcttgattg taattcccta gatttgataa ttgatcatgt ggcacgccaa taaattggtc
    32161 gatcatgaaa gaaagcgcaa tagcgattcc tccaccaata acgaaaggta acatgtgtga
    32221 tacgccgttc atcaaatctt tgtaaattct ttgtccgata gtaccatccg catttccttc
    32281 ttctgaactg ctttcttttc catcaccatg gaatactggt gcagtaccac taagggctag
    32341 attgatcaac tcttctggtt tacggatacc atcactgaca ggacgattga tcagttcttt
    32401 tccgtcaaag cgattcatct ctactttttt gtctgctgca acgattacac cttctgcacg
    32461 ggcgatgtct tcagcagtca atcggttttt gattccttct gaaccgttcg tttcgacttt
    32521 gatctccaca cccatttctt tcgctttttt cttcaaagca tcttctgcca tgtaagtgtg
    32581 agcgatccct gttggacaag ctgtaacggc aacaacgaat tttctttctg gatgagcaga
    32641 agcttctgca gcttgtgttt cttgttctgc tttttcttct gcttcttttt gctcttcagc
    32701 tgtttggaac aagtcttgga cttcttctgg tgtatttgct tcttttaatt ttcctacgaa
    32761 atctgcatca atcagcaatc gagatagtgc tgctaatgct tgcaaatgtg tgtcgtttgc
    32821 gccttctgga gctgcgatca tgaaaaacag ataagttggc tgtccgtcta aggcttcata
    32881 atctactcct ttttggcttt ttgcgaataa aactgttgct tctttaactg cactgttttt
    32941 cgcgtgaggc atggcgattc catcaccaag acctgtagaa gtttgtgctt cgcgggctag
    33001 aattccttct ttatatgttt cgatatcgga aatccggccg gcatcaaaca ttttttggac
    33061 catttcatcg attgcgcctt ttttatcagt cgcttgcaaa tccatgatca tcgcttcttt
    33121 gaccattaaa tccttgatat tcatgattat cctccttgaa actaatcaat ttttgtgatt
    33181 tcaacttctg gcagcaactc atcaatcaat tcgcgagctg ccaagtcatc agaaaaagct
    33241 gttgcgcttc cgcaagcaac tccccattta aaggcttcga ttggatcttt cgtacggctg
    33301 taagccccaa taaagccagc aatcattgaa tcccctgcac ctactgaatt tttcaatggg
    33361 cgaactaata cattcgtttt ataagttcct tgttccgtga agagcaaagc accgtctcca
    33421 gccattgaga tcagcacatt ctgtgcccct tcagctagta attttttccc atacggaatg
    33481 atttcttcta cagattggaa ggttgtgttg taaagctcag ctaattcgtg attgtttggt
    33541 ttgatcaaca acggtttctt aacaagtgca ttttttaggt catcacctgt tgtatcaatc
    33601 acgaaatctg cacctttttc tcgaatgata tcaatcagtt cttgataaaa tccttttcgc
    33661 aggctagctg gcgcgcttcc tgacaatacg acgatatctc cttgtcctac tgttcctaat
    33721 gcttgcttca actcatcgat ttcttcatct gaaatagatg ggcctaagcc attgatctca
    33781 gtttcagtgt ctgattttag tttgatattg attcttgtat cttcttttac ggttgtgaac
    33841 aaagactgga tttgttcttt ttcaagccac ttcgaaataa attcgccagt gaaccctcct
    33901 aaaaacccta gtgctgtaga ttgagaatcg atccgtttca aaatacggga aacattgatc
    33961 ccttttcctc caggtaactt gaagtcttcc ttcattcggt tcaaatcgcc tagttttaac
    34021 ccatctacgc gtacgatata atcaattgat ggattaagtg ttactgtgta aatcattgta
    34081 ttgcctcctt aattgttgtc ttttcacgga attcattaac gaattctaat ggacaacgct
    34141 cggttatgat cgtggctgct tccaaaggtg caacagaggt gaaggacaac tggttgaatt
    34201 tggtatgatc aatcaataca aatgtctctt cggaattttt catggctaaa cgtttaacag
    34261 ctgcctcttc tggatccgga gtcgtgaatc cttgttcaag atgggcaccg ttcattccca
    34321 taaatgcttt attaaaatga aaatttttta gttgttctat cgcattagat cctaaaacag
    34381 cattagttgc cagctttatc gttccaccta aaataatggt agcaagctcc aaatcaacta
    34441 aatttgccgc atgttgaacg gaatttgtca ctactgtgat ctgttttcct tgcagaaaag
    34501 gaatcatttc taacgtagta gagccagcgt ctaaataaat gacatcttcg tcattaatgg
    34561 tttcagctgc cgatttggcg atttgctgtt tttcgtgaac gtttttgagt gatttttcac
    34621 tcatattctg ctcaaaattc aataattgct ctttcttagc tccgccatga actctttcta
    34681 gaaacccact attctccagc tcttgtaaat cacgtcttat cgttgattca gaagcattca
    34741 aaaggtttac aagatcttgt gatttcacga ttttctgctg ttcaagcaag tctaggattt
    34801 tttgtctgcg ttcctctgta agcattttca cgatcacctc aatacaaata atagcatatt
    34861 ttttttctac tagcaatata aatcagtcaa aaacgttcat aaaaattata aatcagtcaa
    34921 aaaaaaataa aaaaccagtc agaaagactg gttttttatt tttttgtcta tcatcaagcc
    34981 gttttatcgt tttttatttt cgcttcttgt ttttttcata taaacagaag caggttttct
    35041 ttccttatga agaagataat aataaagtcc catcccgcca aacatgacca aaaaaccgat
    35101 gactgatcga ataggaaaat taggaacaaa aaagagacag acggataata atgatgccgg
    35161 aaccattatt cgataataga gttgtttatc cattttcttc acctcatatt ttttgtaaag
    35221 taatcaattg ttctaattct cggtaatcta aaaattgcac acgcagatgc tgctcacctt
    35281 tcttgacatc gaattggata cttcgccgct ttttctttat tcgtttgaag gtaacatcag
    35341 agagtctatg tttgatttcc tttaacggta attctgacag ggattctgaa ggaacataga
    35401 ttcttaagga taaaagatac tcttgatatt tttcatctgt tacctctact tctagatatt
    35461 ccctaatata ataacaataa gcttcgattc cattatccaa agcaacagtc ggatcgataa
    35521 ctttatagca catcatgcgt gatcgattct cgtcaaccca aggaaaatac gcctgagcat
    35581 ttagtgttcc gcgaacacgc ctttctttat aatctccttg ttggatatca taactttttg
    35641 ttacaaaaaa tgaagcattc tttttaggaa caggttgcat ttgtgcattc tgatactgga
    35701 cagattggat cgtatggtca gtgtaaatgt attcaacgaa tggcggtttc aataaaccga
    35761 caccattgtc ccaagcaaga tgatcaaacc caagctggtc cctaaaatag gattgaaaca
    35821 tttgattgtt tggcttttgc cagttaaagg ggtctcgata aagctggata catatgactt
    35881 cgttttctaa ttccttcact ttttctactt gttggatgtc gagaaaaatc ggtttaggga
    35941 tgatttgatg ataatagcga ctataataca tttcccaaca cgaagtgaaa cataaacctt
    36001 ggtaaaaaag atcgtatcct ggaaattggt tgcaatcgac tactacttcc ccgttttgtt
    36061 gtttcatttt aggtaaatgt tcgatttcat cctttttctc aaacaatagc cgttgagaga
    36121 tctgttttgt attgtgatag ataaactcgc tataagctcg catataggcg aacaccccat
    36181 gagcaatcat ttttttctga atgaagtctc gaatcaaaaa ttgcttttct ttgaaaacag
    36241 agatagacaa tacatttttt atcaataatt gattatcttc caagcgaaaa ctacattcgt
    36301 tctcgtcatt tcctacaact aaccaacttt cgtctcttac cgatgctagc attatttttt
    36361 cttgaagctt tcgaagatca tacggatccg cttgttgtgt gaaagagcgg ataaatgttg
    36421 gctggatcaa cagacgtgtc aaaatagccc aatcgcttat ttcctgtttt ttttcttctt
    36481 catttaagtc tgtgagctgg atagtcatca ataatttatc ttcgccaatc attttctcac
    36541 ctctttcaaa tctttttata atttaattat aaccaatgat aaagaaaaac taaaacttta
    36601 tgtcttagaa accaatttgg acgattacat gatagactaa gtaaaaagaa acaaagaggt
    36661 gtttacaatg gggttgaaat ttgaacgtac agataattta gataaacttt tcgaatcatt
    36721 tgctgtcgat ccggaaaaaa cagttgcacc aaaagaagag atcgatcgat ttctaaaacc
    36781 acagccacaa aatgaaaaga aaaaagacaa gaaacaaaag taagttaagc tcaaaaaaag
    36841 acttaccagc caaactttta tgaaagagtt tgactggtaa gtctttttta ttctttttat
    36901 gattcaattg cttgttcgat tgctgcccac gcttcttctt ttccctgttt tgtaacggaa
    36961 gaaaagagaa taaacgtgtc attgcgatca aaatccaacg cttttttgat ttcgctctca
    37021 tgcttgttcc atttaccacg agggatctta tctgccttag tggcaacaac aattacaggg
    37081 atatcataat acttcaaaaa ttgatacatc tggatatctt cttgagaagg cttatgacgc
    37141 aaatctacta aagaaacgac cgcacgtagc tggtctctag tcgttaaata tgtctcgatc
    37201 atctttcccc attttgctcg ctcagttttg gatacttttg catacccata tcccggaaca
    37261 tcgacaaaat gtaatgcgtt ctcgatcaaa taaaaattca atgtctgggt ctttcctggc
    37321 ttaccggaag tacgggctaa attctttcga tcaatcaatg tgttgataaa agaagatttc
    37381 cctacatttg atcgtcctgc cagagctatt tctggcaatt gtgtatctgg atattgtttt
    37441 ggtgccactg cacttatgat aatttcagct tgatggactt tcatggtctc tctccttaac
    37501 aaggtgatga ataaagcaag cggctgtcgc aaaagccaaa atgactttta cggcagcctc
    37561 ttttcatcaa cccgcttttt tctctgaatc tttatagatt actgttggct caccagacaa
    37621 ttcagctgct tcttttgtga taatcacttt ttcgatgttt tcatttgaag ggatatcaaa
    37681 catcacatcc atcatgattt cttcaatgat ggaacgaagt cctcgagcac cggtattgcg
    37741 ttcgattgct tttttcgcaa tggctctcaa cgcttcaggt tcgaattcaa gttcggtatt
    37801 atctagagaa agaagttttt gatattgttt gactagcgca tttttcggct cagtcaagat
    37861 ccgtaccaga tcatctgttg tcaatttctc taaagctgcc ataacaggta atcgtccgat
    37921 aaactcaggg atcaatccga attttagtaa atcttcagga atgatatgct gcatgacact
    37981 ttcgtcttca gaaagttttt ggttttgtgt accaaatcca atcgtctttt cgcccatacg
    38041 attcttcaca atcgtttcga tgccatcaaa tgcaccacct acgatgaaca atatgttcgt
    38101 cgtatcaatc tgaataaatt cttgatgtgg atgtttacgt ccgccctgag gtggcacact
    38161 tgcaacagtt ccttctagaa ttttcagcaa ggcttgttgg acaccttcgc cggaaacgtc
    38221 acgagtaata gatacatttt cgcttttacg cgcaatttta tcgatctcat caatatagat
    38281 gatccctttt tctgcaagtt ccacattgta atcagcagat tgaagcaatt taagcaaaat
    38341 gttttctaca tcttccccca cataaccagc ttctgtcaaa cttgtcgcat ctgcgattgc
    38401 aaaaggtaca ttcaatgttt ttgctaatgt ttgtgctaaa aatgttttac cagaccctgt
    38461 aggtccgatc aagcagatat tgcttttttg cagttcaaca tcatctgtgt tttcttgtgc
    38521 ttgctgattg acacgtttat agtgattgta tacggcaaca gctaacgagc gttttgcgcg
    38581 atcttgtcca atcacgtagt tattaaatac ttcaagaatt tctagtggtt tagggacttc
    38641 agtgaattcg cgtacagctt cttcataaaa ttcttcatca atgatttctt tacataggtc
    38701 gatacattca ttacagatgt agacccctgg accagctacg atctttttca cttcttcttg
    38761 ggttttcccg caaaaagaac agcgtacggt ctcattgcta cttggattgt catacatggt
    38821 cttcacccct taacttgcaa aaagtatgca agaaactatc aaagacttta cagtctttta
    38881 ctatcataac atagtttagt aaaaagaaaa agtgtttaac aaaggatcaa ctgacctata
    38941 cctttgcatt ttttactctt aacaaaaaat aaggaactgt gacaaaatcg tgtcacagtc
    39001 ccttatttcg aataaacaat tttctagaag tagcttcttc agaaatgatt tattacttga
    39061 tttctattct ttcgcttctg tcgctgtacc tgtgatcaat tcgattgctt ttttcattgt
    39121 cacatcatgt tccagcatat cttctgtcaa cacgcgtttg atttgttcga ttggcatgtt
    39181 gtatgtttct gataattctt ggatttctgc attgatttct tcttctgttg cttgaagatt
    39241 ttcagcagct gcaattgctt cgatcacaag atttgttctt gtgcgcattt ctgcttcacc
    39301 ttcgaattgt ttatgcaagt cttcttctgt agaaccagtt aattgatagt acatttcagg
    39361 agagatacct tgacgttgca tgttgttcaa gaattcgtcc attgaacgat gtacttcatc
    39421 atgaaccatc acgtgtggca attcaacgat ttctgcgttt tcaacagcca tacggatcgc
    39481 tgcttcatct tttgcatcgt ccgctgcttt ttcttttgtt tctactaatt ctttgcgata
    39541 tttttctttc aattcgtcta atgttgatac ttcgtcatca acatcttttg cgaattcgtc
    39601 atctaattca ggcacttctt ttgcttttac ttcatggatt gtcactttga agactgcttc
    39661 ttttccagca agatcttctg cttgataatc ttctgggaaa gttacattta catctagact
    39721 ttcacctgat ttatgaccaa ctaattgatc ttcaaaacct ggaatgaatg agcctgaacc
    39781 taattcaagt gagtaatttt cgccttttcc gccatcaaac gcttcgccat ctacaaagcc
    39841 ttcgaagtcg attactactg tatcgccatt ttcagctgca gcatcttctt tgataactaa
    39901 ttcagcttgt gcttcttgtt cacgtttgat acgtgcatcc acgtcagcat ctgttacttc
    39961 acggtcttgt ttttcaacaa ctagatcttt atattgtcct aattttactt ctggtttcac
    40021 tgttacttca gcagtgatta cccagtcttg acctttttcc atgctttcaa catcgatttt
    40081 tggttgagaa acaggatcga tgccagcttc tttgacagca gcttcataag cttctggtaa
    40141 tacagcattc aatgcatctt catataaagc ttcttcaccg tacatacggt tgaatacttg
    40201 acgagaaact ttccctttac ggaatccagg tacgttcaag ctttttttca ctttattaaa
    40261 cgcagttgtt aatccttttt ggatttcgtc ttgcgcaatc gtaaatttta aaacaccatc
    40321 attcgtgcct tttttttcaa atgttgcagt catttaattc cctccgagtt tagaaattca
    40381 ttttatacat caaaaacgtt tcatgcatac ctttaaatag tacactaacc ctttcaaatt
    40441 gtaaacatat tgaatggata gaatccttgt ttttcataac atttttatct ttttcactga
    40501 taaaatctaa tggaagaaga tcatttgctg caaaattttt tctctttttt ttaaaaaatc
    40561 ttgtttttca tcttctgtac cttcatacat cgtttttcct gtacacgcct ctacagtcag
    40621 ttgaaaccag cgcttcgggt cttcgatgta tgtatcatag acaggataaa caattgcaga
    40681 ttgcaacgtg aattcttctt ttaacacgga actaagcagc gcatcttcat tcccgtaacg
    40741 atctgaaaat acacataatt gctggtaact tgcctgctgc tgcggctttg gcagatcttt
    40801 tggtatgact ttgactaact tttcttcaat cgtcaaatag aaaacaggat ggcattcgtt
    40861 caattgccta agtgtctcca gtaattttgc ccttgctaaa atatggacct ctggcgatac
    40921 aataagttta ctcgctgttt ggataaactc atgataagga agctgttcga tctcatcaat
    40981 agacgcaagc tgacgtgcag gttccaacga aggaagttcc tccgatttat tgtggagtgc
    41041 cttgatcttc tgcctctcca tttgtccgga taattcttct gcaaattgga ttttttcaat
    41101 gacccgctgc tcgaaagctt cataaaaatc tgtacttgct atcaattttc tagcatataa
    41161 aaaatcttgt gtactgatta acaagtcaaa ataaaattca gcaaactcct cattttgaag
    41221 atactgtttt tcatgcaaga gtgcttgctt caatgcttct tttttctcgt ccaactcgta
    41281 aagacaaaaa acaagcaggc tattagcctc aaagctttct ttcaactcgt aagctcgttg
    41341 aaataattct tttgcctgct gatattgctg gtttgctagt gcttgttgtg ccactcgcat
    41401 taaattctca tatttttctg gaaaatcaat cggcttcacg cagtgtcctc ctcttgctca
    41461 tattcagctc atgttcaata gtagcaaaac aaaaaaagca tgacaattaa aaataactga
    41521 agcaccctct aaaagctaga ttttagatct gacttttaga aggtgctttc aactgttact
    41581 gctttttatt gctcatcagc tgttgacttc ctggttgaac gctttgatca gattgatcgt
    41641 aacaacactt gtattagtga aaaaatcgcc cagatgttgc tttttctgat taaagactgc
    41701 tggaagatac cctaacatca gcaaaggaaa ttttagaatg aaccggcaag caccttctct
    41761 aactaaaacg gttgaccagc taagttcttc ttcatcaaag ctgatgactc gaataccaaa
    41821 gatcattttc ccaatcgttt ggccatgatt gaatttcgtc aataaaataa aatacgctag
    41881 ataaataagt aaggctgtga atccgtagag actcagataa gaatctgaaa aattaagcca
    41941 acctagattt ttggcaatac caagtgttaa acgcgtaata gctccgatac acaaaagatc
    42001 gaccaagaag gcaaacattc ggataaaaaa acctgcatag aaatacttag gaaaatcatt
    42061 tgcttttggc ggttcatttc ttgtgtccgt atactttttc catttacgtt gtttcttttt
    42121 gatttcttcg ggcgtcaaag gctctctttc aaaagagctc agtacctttt gggaaaagtt
    42181 ggagtcaggt cctacagcag gctgctgttg cttatctttt tcttgttcct tattttcatt
    42241 tgaaagctct gacatgctta ctcacctccg taataataca ttgcttttgg tgattccgct
    42301 gtccctagat tctcaagaac ggataagatc tgactagtct cagaaggctt caatccctgg
    42361 aattctgcta gtttacttcc taaccatgta ctagcaaacc cagatggcgt ggaagaatat
    42421 tctactagtt ccgcatcttc aagctgttgt tcctttcgta tagcttttaa agcatcttca
    42481 ggaaatccca attcatcgac taaacctact tcttttgctt ggacaccatc ataaatcctt
    42541 ccatcagcaa tttttttgac ttcttcttca gatttattcc gtccctcgct gacgattgct
    42601 acaaaacgac tatatgcgct gtcgatatat gcttgaagca ccgcttcttc ttcttttgtc
    42661 tctggtcgcg ttcctgaacc catatctttc aacgctccgc ttttaactgt tgtatcttcc
    42721 acacccagtt tttctagaag tcctgaataa ttcaatcctg acatgatcac accaattgag
    42781 ccggtcactg tttcttctgt tgcaaagatt ttatctgctt gagcagaaat atagtagcca
    42841 ccgctagctg ccatgttttt catgctgaca tacattggga tatcatgttc tttacgaatc
    42901 gtatccaatt tcttagcgat ttccgcactt tcgtagacac cgccaccagg agaatttact
    42961 tctaaaaaga tacctttgac tgttggatct tcttcaatcg ccttcagttg ttccataaaa
    43021 tttttatgat tgtaaccttc agaagcaaat aaacctgaag accctcccgc agcaatcgtt
    43081 ccatctactg ataattttac gattttttgc gtactgtctc cctcttccaa cacatttgga
    43141 gctagttctt catccccata taaccaactg ttcaatccct caatttgatc gttttcctga
    43201 ggcttagtca aactagctga aactaacgaa accacaaata atcctgcagc aatcagaact
    43261 gctgcccaac gtcttttatt cattctttct cctttctaat acaagttttt tatgacgatc
    43321 acagtttctg actgctcaga agtgtcgaac cgctctgctt tttcttcccg cgcatacccg
    43381 gaaaacgact cttttgttaa ttcatcttct accgtaaact cacgagcttg agcaacttcc
    43441 aaggcatcag gtgcgatttc ttggatgcta tcccagccag tatctaaaaa tattttcgta
    43501 tcatctgata caccagctga tgcttcgaat ttggacaatt gtttttttaa cattcccagt
    43561 gtcatgatct caatttttga atccattttt actcctcctc tatccgatta ctttctattg
    43621 taccgaactc ctgaaaaaga tgatagataa aaacccatta ttttttgaca taaaaaggac
    43681 atttgacaaa gaatttgata tataccaatt tattttttat tctttttttg ttgacaagaa
    43741 cattcgcaaa aggttatgat tttaaatgtt tccagataaa aaacaaaaag gaggatagtt
    43801 atacctttta ggatagcgcc aggtctgtgt aaacagatga cgaggagagg gtttatcgaa
    43861 agattcggcg gatggcccta gggactgcac tctacagaaa atccacaaaa aatgtctgca
    43921 aagataaaac aaaaggattt tcctgcagct ggaagcattc atacgaattc attatagaaa
    43981 gtaggatgtt ttttatgtgc ggaattgttg gaatgattgg attaaaaaat gtaacaccag
    44041 gattgattga cggtctagaa aaattagaat accgtggtta tgattcagct ggtatctttg
    44101 tctcagatgg cacaactgat tatctggtca aagcacaagg cagaattcaa aatttgaaaa
    44161 acaaaataaa tattgatact acaggaaata taggtattgg tcatacacga tgggcaactc
    44221 acgggcaacc ttctgaagaa aacgcccatc cacatacttc ccaaagtgga cgcttcgtgc
    44281 tcgttcacaa cggcgtaatc gaaaactttg aagaacttaa aattgcttat ttagcaaatg
    44341 accactttat tggtgagacc gatactgaga ttatcgctca tctaattgaa actttcgcga
    44401 aggatactac aacgcaagag gcgtttttaa aagctttgcg ggtcatcaaa ggttcttacg
    44461 ctttcgcttt gattgatcgg acagcacctg atgttattta cgtcgctaaa aataaaagtc
    44521 cactattaat cggactcggc gatggattca atgtgattgc cagcgatgca atggctatgc
    44581 ttgctcatac gaaggaattc gttgagattg aagatgaaga gatggttacc gtcacatcag
    44641 aaaaagttat tattcaaaat tttgcaggtg acatcgtaga acgttcttca ttcgaagcac
    44701 aagtagatgc ttcagatatc gagaaaggta cctatccttt ttatatgctg aaagaaatcg
    44761 acgaacagcc tattatcatg cgtcgtatcg ctcaaaaata cgtaacagaa gacaatcgtg
    44821 tctctcttga tcaaaagctg gtcgatacat tgtctgatag cgatcgaatc tatatcgttg
    44881 cttgcggaac tagttaccat gcggggttag caggtaaaca gactttggaa aaattgacac
    44941 agattcctgt tgaggttcat ttagccagtg aatttggcta taacacacct cttctttcaa
    45001 aaaaaccatt ctttattttc ttgagtcaaa gtggcgaaac agcagacagc cgccaagtgc
    45061 tggtaaaaat caaccgtctc ggctaccctt cacttacgat tacaaatgtt gctggttcta
    45121 ctctttcgcg ggaagcatcc tttacactgc tattgcatgc tggacccgaa atcgcagtag
    45181 catcaacgaa agcttatact gcacaaatag ctgtactaac tttattagcc aaagctatcg
    45241 gtgataaaaa agaatttgaa acttctcttg tattcgatat cactcatgaa ttgagtttag
    45301 ttgctaatgc gatggaatcg gtcatcgccc aaaaagatta tttagaagag ttggcagaag
    45361 tatatctcag cgatacaaga aatgcctttt acattggacg tggtgctgac tacaacgttt
    45421 ctcttgaagc tgctttgaaa ttgaaagaaa tcagttatat ccaggcagaa ggttttgccg
    45481 ctggcgaatt gaaacatgga acgattgctt tgatcgaaga aggaacacca gtgatcggaa
    45541 tcatcagtga agaagtgact ggtgcgcata ctcgaggaaa cctgaaagaa gtcgaaagcc
    45601 gtggcgctaa aacactagtt attgtctcag agggattaga aaaggaaagt gaccagctgg
    45661 tgctaccaac tgtccaccct tatctaacta ctctaaccac agtcgtaccg actcaattac
    45721 ttgcatatta cgcaacgcta ttacggggat tcgacgttga caaaccacgt aatttagcga
    45781 aatcagtgac agtagaataa taaaagaaca aggaccagtg tcagacatct gacactggtc
    45841 cttgttcttt tattttttat gaaaatatct cttaatccag tccgatttct tctttgacta
    45901 ctgccgcgat tttatcgaca tagtaattaa ctttctcatc agtaggcgct tctgccatca
    45961 ctcgaagtaa tggttctgta ccggaaggac gaactaggat tcttccttct gatcccattt
    46021 cagcttctgc ctcttcaatc acttttttga ttgccggaac atccatcgca ccgtttttat
    46081 cacttacgcg gatattcact aatttttgcg gatagattgt tacctctgct gcaagctctg
    46141 ataatttctt acctgtctgt ttcatgatat tcagtaactg aattcctgaa agcatcccat
    46201 ctccagttgt gttaaaatct aagaaaatca tgtgcccaga ctgctcgccg ccaaagttat
    46261 aatcgttttt gcgcatttct tctacaacgt aacgatcacc aacttgtgta acgacatctt
    46321 tcaaaccgat tccttccacc gctttgtgga atcctaagtt actcatgaca gtcgtaacga
    46381 tcgtatcttt tttcaatcga tttttttctg ccaagtattt agcacagata aacatgattt
    46441 tatcaccgtc gacaatgttt ccaagttcat ccaccgcaat aatacgatcg ccatcgccat
    46501 cgaaagctag tccagcatct gctccttttt caacgaccat ctcagctaaa cgttctggat
    46561 gggtagagcc tacgccgtca ttgatattta acccgtctgg atttgtgccc attgtataaa
    46621 aatctgtttc aagatcggca aacaaacgat taacacttgt tgaagttgct ccgtttgctg
    46681 catcgacaca aatggtcaaa ccggaaaggt caccgttgat cgtttgtaca aggaattgag
    46741 aatatttcaa taagccttct ggaaattctt ctacagttcc caatccttca gctgatggac
    46801 gaggcaattc atccacttca gcatctaata atgcttcaat ttctagctct tggtcatcta
    46861 ctagtttaaa accatctgca ccgaaaaatt tgattccatt atcttgtgct ggattatgag
    46921 aagcagaaat catgacccct gcgcttgctt tttgcaaacg tgtcagataa gcaacacctg
    46981 gtgtagagat cacacctagc tggaacactt cgatacccac cgataaaagt cctgaaatca
    47041 gcgcttcttc taacatttga cctgagattc ttgtatcccg ccctactaac acgcgtgggc
    47101 gttttccttc ttcttcatgc tggcttagga cataacctcc gcaccgtccc agcttaaatg
    47161 caagttctgg cgtaagttcc ttgttggctt caccgcgtac gccgtctgtt ccaaaatatt
    47221 tacccatagt tgtaaaatta ctcctttaac ttaattttct gaggaatttg ttctgctcat
    47281 ttgtgttgat tcgcttgttt ctgatgtttc ttctgtactt gtggtcgagc tgcttgtccc
    47341 agattccgaa ctggaagaaa tggttgttgt cgatggttcc gttcctgaat cagcagatga
    47401 aatacttcta ctcgtacttg tattttcttg tgtccgactg ctcgtcgaag cagagctact
    47461 gcttgacgtt tgcaacacgg gtgtaatctc cacactaact gattcgggct tgatacttaa
    47521 cccctcttct actggtattt caattttacg agttgttcga tgattgatac ctgagacatt
    47581 gatcggtaca gcaatactgt caccgatcga tgcaagcaaa tcgctagagc cgcttacttc
    47641 tgcttccagt tttgaaaaac tgaaatcata gctttctacc ccggcagctg gtgttccttg
    47701 ctgtatccca taaagtcgta ccgacttgct tggcttgatc gaatcagctc tcactgttac
    47761 ttgcactgga tcagagacga tacttaatac ttcccctgct gcattcagcg cctgtattgg
    47821 gacctcacta ttaatcccat catctgtgac catcgaagcg tccacatcag ctactactcg
    47881 tttgatttct gccaatgttt tgtcacctgt agtgatagag acttttttag gactaacgga
    47941 agtatcatca agttcatatc cagatggtgt gaacgttgaa ggaatcaacg tttccacatc
    48001 gaaatctttt attacctttt tctcaaccgt tactgtaata gtcgatggtt cgatctttgc
    48061 tgtgacagca ctgctcaaat tttgaatttt tagcggaact tcatgtgttc cttcgcccag
    48121 cttagtgata tctgctgtga cgcggaacat ccgtgtatct tcgtttgcct ctgcattcaa
    48181 ctgaactcgg tttgcactgg aaagtttgac agtcactgca ttttcatatc catggatata
    48241 gtagcgcttt gaatcataag aaatcttgat cggcaccccc gtaactgttt gattatacgc
    48301 ttcatttcca gaaagcgtat ttggaacgga ctggccattt gcattgaagt acagcgcaat
    48361 actgaacagc aatgcaataa aactgtaaag aatgcttgtc cgtttctctt ttgagatcat
    48421 ttgcgatccc ctcccttagt cataccgtct aggaaagatt gaatcacgtt tttcttgttt
    48481 tttgttttct cttcagcaat tagttctcta cgcaagattg ccaaatattc ttgctgggat
    48541 aagccagcca tcagttcatt atctaacgtg atgctgacgt ccccggtttc ttcagagaca
    48601 acgatggtaa tcgcgtcact gacttcgctt ataccgacag ctgcccggtg tcgagtcccg
    48661 aattctttag ggatcaacat actttctgat agaggaagat aggcgcttgc tactgcgatc
    48721 tttccttctt ttacaataac tgcaccgtca tgcagcggtg tattaggaat aaagatattg
    48781 atcagcagct ccccagtgat atctgcatct aaagcaatcc ctgtctcaat atactcatct
    48841 aatccagtat gacgttcgat agtgatcaac gcaccgattt ttcgcttgga catatattgg
    48901 atggctttgt caaaagacaa gatcatcttc tcatcttcct gctgttcgct ttttgattgt
    48961 ttgaaaagcg aactgcgtcc aagatgttcc agtcctctcc ggatttccgg ctgaaaaatc
    49021 acgatagcag ctatcacccc gtacgtaatg acttggttca tcagccaaga caaggtgtgc
    49081 agaccaatga tttccgctac gattcggata acaataaaaa tagctacacc tttcagaagc
    49141 tgaatcgctt ttgtgcctcg cagcagttgg atcagtttgt atacaagata ccagacaacc
    49201 agaatatcaa gtatgttcac caaaaaattc agagagaaca agtcaccgga aaacatttgc
    49261 tgccaatagt ccaaattaaa aagttccccg atttgaaatg acataaaaag cctctcttct
    49321 cttttgagat ttcctctcta gtatatcata tgttttaatt ttagaggctg atatgctcgt
    49381 atttttcttg agaaaaacgc ataattttca agaaaattgc aagtatgcgt tttatttttc
    49441 tctgcaataa atatttttaa acttcagaga attattcatt atattttttc tgttattttt
    49501 cctttttttc ttttgtagct tgttccattt ttttacgtcg ttctgctctg gcagctcttc
    49561 tagcagctct tctttcttct gcttcaaggt ccgtcgtttt ttcttttcta gtcttaacgg
    49621 gtacatcgat agattcctct ggttcaagtc gtttcttgat tttttctaat tctcccgtca
    49681 gtgcagcata gttgtaaaaa cgtgttttag cgtcgtggac agtcttttca aataaagctt
    49741 ttgcaagatt tggatttgcg ttatctagcg aggaaaagcg aacttgtttc ctcatgaaat
    49801 caggcatagc agagaaatca ggttttttgt agtccaaagt cattgggttc ttgttttttt
    49861 cgaccattgt agggttatat cgatataatg accaataacc agaagaaaca gcttcttttg
    49921 cttctttaag cgattctttc attccgccaa gcaagccatg tgtgatacaa ggcgtatagg
    49981 caataatcag agaaggacca ggatactttt ctgcttcttc aaatgccttg atcgtttgca
    50041 tttgattggc acctgaagca atttgcgcca cataaacatt tccatatgtc atcgccatca
    50101 ttcctagatc tttctttgaa gcatactttc cactagccga aaatttagcg attgccgaag
    50161 ctggtgttgc ttttgaggtt tgaccgccag tattggaata gacttcgtta tccaaaacaa
    50221 ggatattcac gtctgctccg ctggcaagta catgatcgat cccgccaaaa ccaatgtcgt
    50281 aagcccatcc gtctccacca atcatccatt ggctgttttt tacgaataga tcctttctct
    50341 tgtatatatc ctccagttca gggaaaattg atcgttcgct ttcgatcgca gcttgcagtt
    50401 ttgctgctct ttgctgcgtt ccttcactct caaacatgtg tactaaccag tcttccatca
    50461 attctcgtag ttcttctgat acttttggca atacttcttt gattgaactt gccaaagcct
    50521 cgcgacgagt ctgtgccgct agaagcatac cataaccaaa ttcagcatta tcttccaata
    50581 aagaatttga ccatgcaggc ccttgaccac attcattggt cgtataagga gaagcaggag
    50641 aagcaccgcc ccaaatagaa gaacagcctg tcgcattcgc gatcatcatt cgatcaccga
    50701 acatttgtgt caataatttt acataaggtg tttcgccaca gcctgagcaa gcgcccgaga
    50761 attcaagaag cggttgattg aattgtgtag acaagacagt attctttcct ttgactggat
    50821 ttgctttttg tcttaatgtc attgcaaaag cccaattcac agcttgttct ttctgttctt
    50881 cataaggctt catcactaaa gctttttctt ttgcgggaca agcctctaca cacagaccac
    50941 aacctgtaca gtcttccact gaaacttgga tacggtattt tacgccgtct gcgcctcgca
    51001 tttctctcac gataaatcct tctggcgctt cttccatttc ctcttcatct gctaaaaatg
    51061 gacggatggc agcatgagga caaacaaagg cacattcatt acacatcgta cagcgatcac
    51121 taatccactc aggtacttct aaagcgacgc cccgcttttc catagaagcc gttcctaaag
    51181 gcatttcgcc agttgtcatt ccatttgcta caagtgtacc aacagagagt tgatttcctt
    51241 cctgtgcatt cactggttca agtatttctt gtatataacc agtgttcctt ttattgattt
    51301 tttcaggcat ctccaacgtt ctccaatggt ctgggatcgc gacttgatga agcagttcgg
    51361 tcgtccgatc gattgcttgt atgttttttt ctacgatagc cattgattta tgtccatagc
    51421 ttttcagggc ttcttctttt agcagcggaa gcacctcatc aaaaggaatg atgtcagcta
    51481 atttaaaaaa agccgtttcc attgccgtat tgatccggcg tcccaaaccg acttcttggg
    51541 ccaatctcat ggcattgatc gtatagaatc taatgttatt ttcagccaag taacgtttta
    51601 attttaaggg cagatgcgtt tctaattgtt cgtcgctcca aagagtattc aataaaaaaa
    51661 tgccacctgg cttcaatcct ttgaccaagt catagctatg caggtaagct ggtgtgtgac
    51721 aggcaacaag gtccgcatgc tcgacaagat acgctgaacg tatcggcgta tctccaaaac
    51781 gaagatgaga gacagtcaga ccgccagatt ttttcgaatc ataataaaaa tagccttgcg
    51841 catatttatc ggtgtgatct ccaataatct tgatagctga tttatttgct ccaactgttc
    51901 cgtcagatcc aaatccccaa aacttggcct ggaatgtttg cggttctgtc aaatccagac
    51961 tttcctttgg ttccaaagat tgataagtca catcatccac gatccctatg gtaaaccgtt
    52021 ttttgcgtat gcttgggtct tttttcaatt catcaaaaac tgctgagatt tggtcgggag
    52081 tcacatcttt agaccccaat ccgtagcgac caccaataac agccgggcga agttcagaat
    52141 catataacgc actttgaacg tctaataaga gaggttctcc acctgcccct ggttctttac
    52201 ttcgatcaag aacagcaatt gtttttactg tatgaggcaa cttttgcacg aatgtttcca
    52261 ctggaaaagg acgataaaga tggatattca aaaaacctgt tttccgtccg tttgcattca
    52321 agtaatcaat cgtttgttca atcgtctgtg caactgatcc catagaaaca atgacttctt
    52381 ccgcatcgtc tgctccgtaa taagtcacca agtcataaga agttcctcgt aatcggttga
    52441 tctctttcat atatttttga acgatttcag gtaatttatc ataatagggg ttgatcgttt
    52501 cccgttgttg aaaatggatg tctggattct ggttcgtacc actaacagtc ggatgattgg
    52561 gattcatccc tcgtttacga aatgaagcta atttctcctg attgatcaga ggagctaatt
    52621 cttcatattc caaaacttcg attttttgaa tctcgtgact cgtacggaat ccatcaaaga
    52681 aattcaaaaa agggacttct ccttcaatag aagcaagatg ggcaacaggt gctaaatcca
    52741 tcacttcttg tacactgctt tcagctaaca tagcaaatcc tgtttgtctt gcagccatca
    52801 cgtctccgtg atctccgaaa atattcaacg catttgtcgt cactgcccga ctagcaacgt
    52861 ggaaaacact tggcagtagt tccccagcaa tcttgtacat attcggtatc atcaaaagca
    52921 gcccttgtga agcggtatat gtagttgtca gtgcaccagt tttcaatgat ccatggacca
    52981 caccagctgc tcctgcttcg gattgcattt ctactacttt tactggttga ccgaatacgt
    53041 tttcttttcc tgctgctgac cattgatcta ctaattcagc catggtcgaa ctgggtgtta
    53101 ttggatatat tgcagcaatc tcggtaaatg cataagaaat atatgcggct gccgtattgc
    53161 catccattgt tttcattttt cgcatgattc atcatccttg ttcatattct ctcttattat
    53221 ctgttccatt ataacgtaaa acatgccatt tttttctttt atttataaaa atattttaca
    53281 tgtatctatt tgaattggtc atctaatttt tagacaaaca gtctaaatcg ttgccttttt
    53341 ttgtcagaca tgtataaact ataagcgaaa gaggtgtttt aacatggata tatcagtgat
    53401 tgatgcaacg aaaacaaata cacaaaaagg aatcctttat ggttcatcga atgctcctaa
    53461 aaaaatggtc gaattcatca acttagcttg tccgtattgc cgacaatggt ttgaagaatc
    53521 ttatgagcta ttagaagaag cagtccaaag cgggcaattg cagcgagtga tcaaattgtt
    53581 cgataaagaa aaagaaagct tacagcgagg gaacgtgatg caccggtatc tcacaatcag
    53641 cgatggccaa aaagcgatca aagagatcaa acaaatattc gatactcaag acgagtggaa
    53701 acatctttct ctccaagaag tagcggattt tgcagtagat aaactaaagc tgacagaaca
    53761 aaaagacgaa cagctgagcc aagctgtcat caacgaagct gagcaagcac atattcgatt
    53821 tgttccaact gtcatcttgg gtaaagaaat ctttgatgaa tctatttcaa tagaggaatt
    53881 aaaagaattg atccaaacaa aataaaaaaa taaggagcgt gggacaaaag taaagaacac
    53941 ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagcaactc cttcttattt
    54001 cttggagtta aacgcttctg tcccagcctc cttattccga ataaatggtg tccaaaagct
    54061 tgcatctgcc agattatctc atttcttatg acaagctaag caaattatct ccaccaaata
    54121 atttattcat atttttgact tgcgcatact aactaattct taaattttca tacaaaattt
    54181 ctcaatttcc cttaacgagt aatatttatg tacggtgatt ctcactagta tcccaatcac
    54241 tttttaagtt atcccgattt caattctact tcaaaaatag ttgcttttat gtatgctgtt
    54301 agttgattag atggtcattt gttgagctag gtcgtttatt ttaaacaagt tgtttcttta
    54361 gcttatacct atctcgtcta ccaaacgcaa gatggcaaac acaaatgcat acgcatctgt
    54421 atttgccact ttgattcgta taaagctaac aactattaac tcctctcagc taatagttgc
    54481 tctctttttt attcagattc atttttgctg atcacagcct agtatttcaa tggttttgaa
    54541 cactaaacca aattgttcca ttacaacatt cactggctga tgaaatggat tagtagattt
    54601 cgattattca aagcgatttt ctattggtgg atcgtgttcc caactaataa gacggattcc
    54661 aaacctatcg tacagatctt ctaaaccact tataaatttt gcacctattt caggtgtttt
    54721 attgcgtgga tattctattg ttacgccatt aaatggatac caattaggat taggctctct
    54781 ttttttctgt acatctccca tagaatagtt agcactttct gggacataat atattcttcg
    54841 accatcctct aattcatcta ataagatatt tcgtgatcgt ctatcacttg ctaaatgtag
    54901 acgccacgtg cgataccatg ccatgctacc tgcatctgca gaaaatccat caaataatgc
    54961 aacaattgta tcttctatca caatcctttc cccatctact tcatattcta atctaaaatc
    55021 aaactcccct cgggtaattt ctggtcttgg tggcttagga cccaatatta ttctacttaa
    55081 aaccgcattc gttcccagat gaaacacagc tataacaaca ataatgatta atattttgtt
    55141 tctatttgat aaatttctca ttattcgcac atcacctcta cattctcaca tattagttcg
    55201 taatactcct ttaatatcaa aatatctact ggtcgtaata atattcgcta tgaattccta
    55261 ctactcgtat aagaaaggcc aaatcatcag cttttacttg ctgatgattt ggcttgtttc
    55321 tatttatcgt aattgttttc taatcaccaa aattgttaac aatcggttca gatatttccc
    55381 agttgattaa ggtaattccg tattgtgtca aaacttcttg cgcttctccg aaattaagta
    55441 cagttcttat gtatccactt tctcctaata taaagtctac ctctctttta aaaccttcct
    55501 cgagatttct aagggctata tgtggtttta atgacattga ttgtacattt tcccccatat
    55561 aatattgcgc acttcctcga ctaaaataga ttcctagatc gtccccaaaa ggtagatctt
    55621 ctctaccact agctaatcta cttctccatc ttcgttctct tttcatagaa cctgcgctga
    55681 actcacaccc gttaaactca gctatgacag tatcttcaat tatgttaatc tgtccatcta
    55741 gttcgtattc aatcctaaat ggaaattctc cataggtgat ttccggttcg ggtattttag
    55801 gtgagattaa tgcaaagaaa ggaaatatta agcttggcaa catcaaaaaa actcctccta
    55861 tgatcacaat tcccgatgta atccaaaata taatttgtac aattctcatt gatccattgt
    55921 attccatttt attcccccta ttactattca tatccgccta tctatatcga tcttccaatg
    55981 cagagataat tgcttccata ccttgatttt ttactgagtt acctatgcga gatatcattc
    56041 tccactaagg tgcatcatat tgtctatcta acatgattaa ttctccaata ggtgtatact
    56101 cctccaaaaa caacatttaa tgcatctccc tcgacaacat aagctaccat gtttgctgta
    56161 tatgtgtttg agtctaattc ataagcagat aaatttactg tcgctggatt aaatataatc
    56221 gcattcgtat ttatagctac tgaatttgct gcttcagcac caccttttga atgtccgatc
    56281 attgtgattt caatagtagg atttacgtgg acagccctca ccgtttaccc tgaccattat
    56341 agaaatcgct tacttttttg aactacgcaa caaaggatac aatgctccta acttcactgt
    56401 ctccattttt gtccactaag gaatataaaa aataggcatt aattcattta attcggtcac
    56461 aacacgttac cttttaaatc attatactgt atctcataca ttattatcaa taaactatta
    56521 taaaaatgca aaaaatgctt tatcaatcta ttttgtaatt ggtcataata tcattgaaat
    56581 cataccctgt atcgattttt cctatgaatc tcgtctattt gtgattgacg aaaaagaggc
    56641 aagacagaca ctggaatggt aattttaccc actattttgc tccaactatt tgtaatcgta
    56701 cccacttttg aaataccgag atgatcattt taattgatat gctgattccc aagcaaaacc
    56761 ccaagaaatt aaatgctcta tcgcatctag cttcttgggt tttgcttata taaattccaa
    56821 catttaaatt ttttatgctg ttcggtctca gaaatgccgt ttttgcttat atcacaatat
    56881 caagatgtca aattattcgt gttgccgatg actttgatta atttttttca aattgtttat
    56941 taagactaat gctgtctttt tgcagatacc aatgttcgaa ctttttaaca ttatcatatg
    57001 atcctgcaag ttttaacttc ttaccattag atgttatcaa agttataatt aaccttgcct
    57061 ttttcccatt aggatctttt attttatgaa ttttctgcag gtaatctctc ctaatggcag
    57121 ctatttttac ctcatcagtg ctgataaacc attgatcatc cacatacata ctgcctgtta
    57181 tgttgtgttt ttccatttct tccgagaacg aaaagtttaa ggctcgttct tacttccaaa
    57241 gttgaatgag tccatggatt ggacttagtg tgagaattga gaacaacaat aagaatccat
    57301 atcctcccgc aaacatccct aaggctatac tgtctctcca tccataagta ggattagaag
    57361 agaaaagagt caccgtttgt aataatatga aaaggaataa tccagcacaa acaccaaaga
    57421 taacaactcc aaacattgaa tttttataca atgaccattt atttataggc ttttctttca
    57481 ttttacttcc ccctactgtt ctgttcacac ggaatgaatt caaagttcat aagccctcct
    57541 ctttgaacat atgaatacga cttgctaact atactttaaa aaagcttgtt ttaagcggtt
    57601 ataggcgagt tgtaatctct attcatctca ttagcaaata acttgccgaa aagtctactg
    57661 aaattaaagc agatagtgga agaattattt agccaggata gacactattt gtgaaacaca
    57721 tgtctttact tttgaaattc tatgatgaaa agccttggaa tacagtagct cttaattggt
    57781 aatacttttt ccattataaa taacctcttt tcattttata tttatcccct ctaaaaataa
    57841 tatctaatct aatattttaa acttatattt ccctcgctgt gctaatagag cattactaaa
    57901 taaatagaaa ctagaaaaaa gcattacgcc tgccagaata aaccaattat ttgt
//
LOCUS       DAJSBN010000013        57915 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJSBN010000013 DAJSBN010000000
VERSION     DAJSBN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.013"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ONT86_000871"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ONT86_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR2351686.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ONT86_000872"
     CDS             852..1040
                     /locus_tag="ONT86_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR2351687.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ONT86_000873"
     CDS             1053..1610
                     /locus_tag="ONT86_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2351688.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ONT86_000874"
     CDS             1642..1884
                     /locus_tag="ONT86_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2351689.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2225..3439
                     /locus_tag="ONT86_000875"
     CDS             2225..3439
                     /locus_tag="ONT86_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCR2351690.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3470..4897
                     /locus_tag="ONT86_000876"
     CDS             3470..4897
                     /locus_tag="ONT86_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2351691.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            4920..5846
                     /gene="glsA"
                     /locus_tag="ONT86_000877"
     CDS             4920..5846
                     /gene="glsA"
                     /locus_tag="ONT86_000877"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR2351692.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6054..7514
                     /locus_tag="ONT86_000878"
     CDS             6054..7514
                     /locus_tag="ONT86_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351693.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            7735..9498
                     /locus_tag="ONT86_000879"
     CDS             7735..9498
                     /locus_tag="ONT86_000879"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR2351694.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            9582..10040
                     /locus_tag="ONT86_000880"
     CDS             9582..10040
                     /locus_tag="ONT86_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR2351695.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10149..10832
                     /locus_tag="ONT86_000881"
     CDS             10149..10832
                     /locus_tag="ONT86_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR2351696.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            10979..11593
                     /locus_tag="ONT86_000882"
     CDS             10979..11593
                     /locus_tag="ONT86_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCR2351697.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            11609..11815
                     /locus_tag="ONT86_000883"
     CDS             11609..11815
                     /locus_tag="ONT86_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351698.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            12354..12911
                     /locus_tag="ONT86_000884"
                     /pseudo
     CDS             12354..12911
                     /locus_tag="ONT86_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            12969..15008
                     /locus_tag="ONT86_000885"
     CDS             12969..15008
                     /locus_tag="ONT86_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCR2351699.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(15117..16268)
                     /locus_tag="ONT86_000886"
     CDS             complement(15117..16268)
                     /locus_tag="ONT86_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCR2351700.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(16422..16952)
                     /locus_tag="ONT86_000887"
     CDS             complement(16422..16952)
                     /locus_tag="ONT86_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR2351701.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(17051..17401)
                     /locus_tag="ONT86_000888"
     CDS             complement(17051..17401)
                     /locus_tag="ONT86_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCR2351702.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(17558..19087)
                     /gene="ahpF"
                     /locus_tag="ONT86_000889"
     CDS             complement(17558..19087)
                     /gene="ahpF"
                     /locus_tag="ONT86_000889"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR2351703.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(19097..19660)
                     /gene="ahpC"
                     /locus_tag="ONT86_000890"
     CDS             complement(19097..19660)
                     /gene="ahpC"
                     /locus_tag="ONT86_000890"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCR2351704.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(19804..20466)
                     /locus_tag="ONT86_000891"
     CDS             complement(19804..20466)
                     /locus_tag="ONT86_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2351705.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            20650..22296
                     /locus_tag="ONT86_000892"
     CDS             20650..22296
                     /locus_tag="ONT86_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR2351706.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            22287..23369
                     /gene="uxuA"
                     /locus_tag="ONT86_000893"
     CDS             22287..23369
                     /gene="uxuA"
                     /locus_tag="ONT86_000893"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR2351707.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(23477..25030)
                     /locus_tag="ONT86_000894"
                     /pseudo
     CDS             complement(23477..25030)
                     /locus_tag="ONT86_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            25178..26319
                     /locus_tag="ONT86_000895"
                     /pseudo
     CDS             25178..26319
                     /locus_tag="ONT86_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(26445..27524)
                     /locus_tag="ONT86_000896"
     CDS             complement(26445..27524)
                     /locus_tag="ONT86_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2351708.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(27627..28067)
                     /locus_tag="ONT86_000897"
     CDS             complement(27627..28067)
                     /locus_tag="ONT86_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2351709.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(28124..28534)
                     /locus_tag="ONT86_000898"
     CDS             complement(28124..28534)
                     /locus_tag="ONT86_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR2351710.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(28714..29148)
                     /locus_tag="ONT86_000899"
     CDS             complement(28714..29148)
                     /locus_tag="ONT86_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2351711.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(29248..30096)
                     /locus_tag="ONT86_000900"
     CDS             complement(29248..30096)
                     /locus_tag="ONT86_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2351712.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            30485..32251
                     /locus_tag="ONT86_000901"
     CDS             30485..32251
                     /locus_tag="ONT86_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCR2351713.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            32353..33612
                     /locus_tag="ONT86_000902"
     CDS             32353..33612
                     /locus_tag="ONT86_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2351714.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            33759..35060
                     /locus_tag="ONT86_000903"
     CDS             33759..35060
                     /locus_tag="ONT86_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2351715.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            35063..35914
                     /locus_tag="ONT86_000904"
     CDS             35063..35914
                     /locus_tag="ONT86_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2351716.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            35927..36742
                     /locus_tag="ONT86_000905"
     CDS             35927..36742
                     /locus_tag="ONT86_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCR2351717.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            36893..38059
                     /locus_tag="ONT86_000906"
     CDS             36893..38059
                     /locus_tag="ONT86_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2351718.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            38289..38936
                     /locus_tag="ONT86_000907"
     CDS             38289..38936
                     /locus_tag="ONT86_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR2351719.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            39020..39964
                     /locus_tag="ONT86_000908"
     CDS             39020..39964
                     /locus_tag="ONT86_000908"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCR2351720.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            40099..41550
                     /locus_tag="ONT86_000909"
     CDS             40099..41550
                     /locus_tag="ONT86_000909"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCR2351721.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            41571..42407
                     /locus_tag="ONT86_000910"
     CDS             41571..42407
                     /locus_tag="ONT86_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR2351722.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            42407..43066
                     /locus_tag="ONT86_000911"
     CDS             42407..43066
                     /locus_tag="ONT86_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2351723.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            43192..43881
                     /locus_tag="ONT86_000912"
     CDS             43192..43881
                     /locus_tag="ONT86_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351724.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            43887..44843
                     /locus_tag="ONT86_000913"
     CDS             43887..44843
                     /locus_tag="ONT86_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCR2351725.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            44887..45399
                     /locus_tag="ONT86_000914"
     CDS             44887..45399
                     /locus_tag="ONT86_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2351726.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(45655..46203)
                     /locus_tag="ONT86_000915"
     CDS             complement(45655..46203)
                     /locus_tag="ONT86_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2351727.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(46292..47473)
                     /locus_tag="ONT86_000916"
     CDS             complement(46292..47473)
                     /locus_tag="ONT86_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR2351728.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(47709..49217)
                     /locus_tag="ONT86_000917"
     CDS             complement(47709..49217)
                     /locus_tag="ONT86_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCR2351729.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            49376..49567
                     /locus_tag="ONT86_000918"
     CDS             49376..49567
                     /locus_tag="ONT86_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351730.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            49770..50687
                     /locus_tag="ONT86_000919"
     CDS             49770..50687
                     /locus_tag="ONT86_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCR2351731.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            50680..51753
                     /locus_tag="ONT86_000920"
     CDS             50680..51753
                     /locus_tag="ONT86_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351732.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            51901..52419
                     /locus_tag="ONT86_000921"
     CDS             51901..52419
                     /locus_tag="ONT86_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351733.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            52400..52792
                     /locus_tag="ONT86_000922"
     CDS             52400..52792
                     /locus_tag="ONT86_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCR2351734.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            52868..53803
                     /locus_tag="ONT86_000923"
     CDS             52868..53803
                     /locus_tag="ONT86_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCR2351735.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            53936..54715
                     /locus_tag="ONT86_000924"
     CDS             53936..54715
                     /locus_tag="ONT86_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR2351736.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            54708..55025
                     /locus_tag="ONT86_000925"
     CDS             54708..55025
                     /locus_tag="ONT86_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR2351737.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            55269..56879
                     /locus_tag="ONT86_000926"
     CDS             55269..56879
                     /locus_tag="ONT86_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2351738.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            57036..57884
                     /locus_tag="ONT86_000927"
     CDS             57036..57884
                     /locus_tag="ONT86_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2351739.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        19269 a        10229 c        11305 g        17112 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttatgaaaa aatatcttta aaaataaaaa aagacgaaaa taaacggttt
     2041 tctataaaaa gaagaccgtt tattttttgt ctttatttgt gtgaaaaacg aaaaagagca
     2101 caacacatca agcttttatt aattgtctgt cttttataaa tgcaagtatt aattcaaaaa
     2161 aacagaagaa aaaaacaact acaccttctt ttatactcgt aatcgtagaa aggaggcgaa
     2221 aaaaatgaat acattaccta tcaatacagg agatacagca ttcatgattt tatgtacagc
     2281 aatggtttgc ctgatgactc ctggtctagc ttttttttac ggcggattag cacggaagaa
     2341 taatatacta acgatcatgt ctcaatcttt tctctccgtt gggataacaa cgattatgtg
     2401 ggtatttggt ggattcggat tggcctttgg acgggatatc ggcggtgtta ttggaaaccc
     2461 ggcagatttc tttttgatga gacatgttac aagcttcccc aacatgtttc atggtgcaac
     2521 gattccattt ttaatgttct ttctttacca actgatgttt atcgtgatta ccgttccttt
     2581 gatgacagga gcttttgcag ggagattgaa tttaaaaggc tatattatac ttgttatttt
     2641 ttggaatctg ctgatttatt tccctgtttg ccactggata tggggtggcg gatttttgga
     2701 tcagatggga tttagagatt tcgctggtgg agcagtcatc cacacgacag ctggattcgg
     2761 ctctttagca tgtatcctca cacttggtca aagaaagatt cctttaggag aaagaagtca
     2821 gcatagcagt cttatggcag ctgctatcgg tacaggactg ctttggtttg gctggttcgg
     2881 ttttaattcc ggtggtgcac ttcgagcaga tgaacaagca gtcaatgcat ttggttcaac
     2941 attcatcgct cttgcatttg cgatgattac ttggctgatc attgcaaaag taaaaggaaa
     3001 tggctttgat tttgtcgacg tactaacagg atccgttgct ggattggcag ccattacgcc
     3061 ttgtgccggt tatgtagaag caaaaagtgc catgctgatt gggatcattg cgggtatcgt
     3121 ctgtcatgct gctgttgatt ttcgtaaaaa gaaacaatgg gatgatgcac ttgatgtgtg
     3181 gggtgttcat ggcatgggcg gattcacagg tacgattctg atagggatct ttgcatctaa
     3241 cagccattta ctaacgaatc caagcagctg gtacttctta gggattcaag tgattggtgt
     3301 catcattact gccgtgtatg cctatgtgct gacaacagtg attttaaaat cggcgatgca
     3361 ctttacaacg atcactacta caaaagaaga acaagaagaa ggattggact ttttcttctt
     3421 tggcaacaaa ctcaattgaa acaaattcaa atagaaaaga ggaagattca tgcaacaaag
     3481 caaaacgaaa attcctgcaa aaaaattaac gttctttggc tttttggcta tgaccatctc
     3541 aatggtcgtt tcattatatg aatatcccac attcgcgacc tcaggcttca gcttagtatt
     3601 tttcttgtta ttaggcggtt tgctatggtt tatccctgtg gcattgtgcg cagcagagat
     3661 ggcaacggtg aaaggatggg aaaaaggcgg tgtctatact tgggtcagtc gcacattggg
     3721 aaaacgattc ggttttgccg caatcttttt ccaatggttt gagataacag ttggctactt
     3781 gaccatgctg tatttcttaa ctggtgcatt atcctatgca acgggtattg gtgctataca
     3841 aaacaataaa tatctgaaat tggcaatcct attgattatt ttctgggcta ttttactctc
     3901 acagttaaaa ggaaccaaat acacttcatt gattgctcgg atcggttttg tagctggtat
     3961 cttgttgcca gcattagtct tgtttggctt agggatccat tatgtcgctt caggcgctcc
     4021 actacaaacg atactgtcta tgaaaacatt gattcctgat ttttcgaaat tgcctacgct
     4081 ggttgttttt gtttccttta ttttagcata tatgggcgta gaaacatctg ccagtcatgc
     4141 aaatgaaatg gaaaacccta aaaaggatta tccattagca atgtttgtat tggttattct
     4201 ggcaatcatt ttagatactt ttggcggatt gaccgtcgct actacagtac ctcaacatgg
     4261 tttgtctctt aatacagggg tgattcaatc gttagaattc ctattaagac acattgatcc
     4321 aaatcttgca tggattgcga aaattcttgc gattcttgtt tgtttaggtg tcatcggaga
     4381 aattgcttct tgggtaacct ctccttcaaa agcacttcac gtagcagcag aagaaggatt
     4441 acttcctgaa tattttgcaa aagaaaacac acacggtgtt cctgctcatt tgatgatcgc
     4501 aaatgggatc gttgcaacaa tctgggcagc cgtctttaca ttaagcggcg gggggaataa
     4561 tctttcattt cttgcagcga tgtcattgac tgttgtaatc tacctgatgt tgtactttat
     4621 gtttttcgcg ggctatttga agctggtttt taaagatgac agtcttgaaa gagcctatca
     4681 ggttccaggc ggaaaagttg taaaagtaat cgttgcttgt ttaggtcttt tgacttcact
     4741 ttttgctttt tgtatctcat ttgttccacc tgctagtatc gcagctggac agcatgggat
     4801 ttatgaaacg attctgattg tttcttatct tatcgtctta gttattccat ttacgatcta
     4861 cgcatgccgt cataaattcc agcatgcaga aaaataaaaa atgaaagctg gttattcaaa
     4921 tggaagtaaa agaattagaa caattgattg gaaaaaattt ccactattat caaacaggac
     4981 aagtggcgga ttacattcca gcattagctg aagtggatcc caaacaatta ggtatggcga
     5041 tttatgattt ggatacaaaa caactgattg agggaggaga cgccgatgtg cgctttgcca
     5101 tcgaaagcat gtcaaaagtt cccgtattgt tattagcaat caaagacaac ggaatagaaa
     5161 aagtctttca aacaatcaac acggaaccaa ccggttttgc cttcaattct atcatgaata
     5221 tggaaatcaa tcatcgcaaa catccaatga atccttttgt taatgcagga gcgattgcaa
     5281 caacctcgtt gattgccggt aaagatgcag atgaaaaatt tgagcgaatc ttgtcattta
     5341 tgaaagagat ctgtgatgat cctaatatca cattgaatga agaaatctat cattctgaat
     5401 caagaacagg tgatatcaat cgctctcttg cttactatat gaaaggtaat caaatgatcg
     5461 aaggagacgt accggagata ctcgatgctt atttcagaca atgctccctc aatgtcaccg
     5521 ctacaggcat agcaaaatta gctgctgtgc tggcaaataa aggaattgct ccttggaatg
     5581 gcaaacaaat cattactgaa gaaagtgcta caatcgtcaa atcaattatg acaactgctg
     5641 gtttgtatga tgaatcagga gagttttccg tccatgttgg tgtacctgca aaaagcggtg
     5701 taggcggcgg gttgatggca gctgtgccaa atcgttacgg cataggtgtc ttcagcccgg
     5761 cactagatcc atttggtaac agcgcagctg gtatccaatt gttgaaagat gtagtcaaag
     5821 aactagacgc tgacatcttt gaataaccat tcttgaaaga ggctggggta aaagtccaaa
     5881 cggctcaaag ctacctacaa tgaggctgaa attgtgagct tgtttgactt ttgtcccagt
     5941 ttcttttttt attggttttt gaaagggctt ttctaaataa aaaggaaaca aaaatacgat
     6001 ttacagatat gattaaataa aatcataaga aaaactggag gcgtgtatca ggaatgctgg
     6061 aaaattacat cgagcgaaat atatttagaa aagtgtatct gtgtgaacaa ttatttgaat
     6121 tccaagaaat agacatcgaa caaacagcta tttctctaag ggtgactaca ccaacgatcc
     6181 ttcacgatct agaaagtcta gccgagtgtc tagaatattg tataaaagaa caagtaagag
     6241 agaagcataa gtataaactc gtattcaaac atggtattgc tctttctgag ctcacacagt
     6301 ttctgtacgg tcaatcttat tttttaaagt ttttaagtta ttattttaat ggtatattca
     6361 cgagtacaga actggctgat cttgaattta tttctttgag caaagtatat acaatcaaaa
     6421 aaatcgtatt ggattttttt aaagaaaatg attatttgaa aaataaagaa atcattatcc
     6481 cagaatttga ctcgcggaac atcttgctag ctttagtacg ttatgtcaat tgggaaggct
     6541 atgaaaataa aaatccgtat gtagaagaag cttgtaacga gttgattgat tatgtagaga
     6601 ctcatttttt taaaagaaga tattcggaag atgaaaagtc ctttatcata cgaggaatag
     6661 agatcgcaat cggaagaaag gcatttcccc ttggactgaa agaagaagat aagaaaagcg
     6721 cagaaacgaa gccgttattt caattagtct gtcaaggatt ggaagagcaa aaaggaattc
     6781 tagacttaca agaagaagat gtctattata ttttttcttt gttcaacagt agaaattata
     6841 ccaatgagaa catggagctg ttaagaaaag atttcgaagt tgtttataaa aattttgttc
     6901 tcaatgacct atcactgaat gaattgattt tacagattat tagtatcgct gaaatcgacg
     6961 atgcagaaaa ttttattttc aagcaatcct ttttacaatt tgtccgtacg ttgtgggcag
     7021 atggacaagt atttttaccg gaaaggatct atcttctatc agaaaaagaa caacttctgt
     7081 acggaaaaat tgtagaaata ttggaagagt ggaaaaagaa aaatcaaatc caactaagat
     7141 ggaatgaaaa tttcattcga aaattcgtca aaagtctttc tttgatcaac agtgaacaag
     7201 cggggagaca ggaaatagaa atttttgttg tttcagaaag ttcagtcaaa caattttttt
     7261 atcgaaccca atttcatttg tatgcggaag aaggcatcgc tgaaataaat cctattttat
     7321 ttcgttcatt agaagaattg cctgatgaat gtctatgtgc gaaaaaaaga gtggttttgt
     7381 gcgatgctgc tagctatcaa aatggattag aaactgaaaa gacaaagatc tatccaatct
     7441 cattaaaagg tgcaaacatc catttgtatc atattgctca ggaacttcac ctattaaaaa
     7501 aacatatttc ttgatgagac cttaattagg agaccttcta caaagctttt taattgtctg
     7561 tagaaggttt ttatttgagt cctgattcgt gcgaagatct acttatataa caagctttct
     7621 ctgaaatttt tttaaaatgc tattgactta gagtcaactc taagtagtag aatagataaa
     7681 gtgtaacaaa tctattttca ttaaaagaag atagtacgga ggagagaatg tattatgagt
     7741 acagtattac atgtattgga tgcaatcaat agccccgcag atgtcaaaaa attatctgtt
     7801 gaggaaatga aacagttggc agaagagatg cgccaaaaaa tcttgcaaaa ggacagcgat
     7861 gtaggtgggc acgttggtcc gaatctaggt gtagtagaat taacgattgc gtttcattat
     7921 gtatttgatt caccaaagga taaaatcgtt tgggatgttt cccatcagtc ttatggtcat
     7981 aagcttttaa ctggtcgaaa aaatggtttt gaaaaaggac attaccgtga agtgagcgga
     8041 tattccaacc aacatgaaag taaacatgac tattttacag tggggcacac atctacatct
     8101 atcagcttag caacaggttt agctaaagcg agagatctga agaaagaatc aggaaatgtg
     8161 gtagcattta tcggtgacgg ctccctaagt ggtggcttag cttttgaagg attgaacaat
     8221 ggtggtgttt tacaatccaa tctaatcgta ttagtaaacg ataatgagat gtctattgat
     8281 gagaatcatg gcagtttata taaaaactta gctgagcttc gtcaaaccaa cggtaaaagc
     8341 agcaataacc tatttaaagc aatgggattt gattatcgtt acgtggataa tggaaacgat
     8401 ctggaaacgg tgatccgttt atttgaagaa gtaaaagata ttgatcatcc gattgtactt
     8461 cacgtccata cggaaaaagg tcgcggttat cagccggcaa tcgataataa aatgaaatat
     8521 cattggcatg taccatttga tctgtccacc ggggaggcaa agcaagtaac ttctggagaa
     8581 acatttaata tgatcatcat ggattatttg gataagaaaa tgcaaacaag tacagctcct
     8641 attgccgcag tcaatgcagc gataccaggc gcctttggac tgaaaacatt ccaagaaaaa
     8701 catccagatc attattttga cgtggggatc gctgaacagc actctatcac tttcgcttct
     8761 gctttggctt ctcagggagt acgtccagtc gtcttccaat caagtacttt cttgcaacgg
     8821 gcatatgatc aattatcaca tgatttagcg atcaacgaga atccagtgat tattttagtc
     8881 ggtggcggaa cgatctcagc tagtgaccga acacaccaag gaacatttga cattccttac
     8941 ttatcaagta tcccgaacat caaatatctt gctccagcaa caaaagagga tatgatctct
     9001 atgctagatt gggcgttcga acaaacagcg cagcctgtag caatccgaat tcctacacat
     9061 ggtgtagaac atggtacagc tatcgcaaat gattatagta ttccagccta tcaaacagta
     9121 aataatggag gaaaagtagc aattttagct ttaggcggtt ttcttgaatt aggaaaagaa
     9181 acagtaaaag cttttagaaa aaaaggaatc gaagcaacgt tgatcaatcc aggaacgatc
     9241 acagaaaatg acgtagaaac attagataaa ttaaaagaaa cacacgaatt agtcatcaca
     9301 ttagaagatg gtagtttgtc aggaggattt ggcgaaaata tcagtcgtta ttatggtgat
     9361 tcttcaatga acgtattaaa ttttggtgca gagaaagaat tcacggatag cgttcctgta
     9421 gggcagcttt atgaacgtta tcatttaaca ccaaaacaaa tcgttgaaga tagtctgcgt
     9481 gtcttgaata ttcaataatt cattatgctc accatccaga taattgttta tactagtatc
     9541 tggatggttt tttcattata aaaagcaaag gggtggatgt tatgtatcag caagtagaag
     9601 aatatgtcaa aaagttccaa gaaaaatatc ctagttatca atcagaaaaa ttaggtttct
     9661 tttcatttgg ggatacagaa aaaatggcag acgagttgag cgaactagtt gtaaaaaaga
     9721 taaagactgg tacatcaagt ggttacgatc tgtataaaca agagaatgag cctttaccaa
     9781 aagttggtca attagacgtg gtcctgaatg gaaaaaatga accagtttgt atcatcaaaa
     9841 atataaatgt gaccatcttg ccttttagtg aagtcacgaa agagcatgct tggaaagaag
     9901 gagaagggga tcggacatta gcttattggc ggaaagtcca tgctgagttt tttaaacatg
     9961 cttataaaga agcgttgaac atagaattta cagaagagaa gctggtcgta tacgaagaat
    10021 ttgaagtaat tttcttttag taagaaaggt ctgctgaaag aaatagctca ttattgtctc
    10081 ctgaatagaa aagggttaca tttttatatg aagctgtatt tttatataac attactggag
    10141 gagatattgt ggatacactg gaacaaatca aaggcggagt cgtcgtttct tgtcaagctt
    10201 tggaaaacga gccgctacac agttctttta ttatggggag aatggctttg gctgcaaaag
    10261 aaggcggagc agttggtatt cgagcaaata cgacagaaga tatcaatgaa atcaagcgaa
    10321 tcgttgattt accggtaata gggattatca aaagagatta cgatgggtca gaagtgttta
    10381 ttactgcgac agaaaaagaa gtggaagaac ttttggagac gcaagcagaa atcattgcac
    10441 tggatgcaac ttctcgtaaa cgtccgacag aaaaaacaac tgctgaactt gtccaaatga
    10501 ttcacgaaca cggtcggtta gctatggcag atatttcgac ttttgaggaa gcgcttcaag
    10561 cgcaagaaga tggttttgat cttttgtcta cgactttagc aggctataca gaatattcac
    10621 gaaagacgca gacacctgat tttgatttat tgaatcaaat catagaacaa gtcgatatcc
    10681 cagtaattat ggaaggacat actgactctc ctgaacaagt agagaaagca tatgaattag
    10741 gtgctttttc agtcgtagtt ggatctatta tcacccgtcc gcaattgatc actaaaagat
    10801 atgtcgaagc cgcaagtaaa gcaaataact aaaaaacacg cacggcacag attcttgaca
    10861 aaaaagaaaa attgatagat agtttaaagc agattctaag aagatgaaaa aagcattact
    10921 tttcattttg tcttcaagat ctgcttttta acgaatttta tagaaagaag gcgaaaccat
    10981 gtttgaacgg agtccttcag aattgacaga acgtgaaaat tacaagttgc tgattggttc
    11041 agtcatccct agaccagtag cagtagtaac gactaaatca gcaaaagata tagtgaatat
    11101 cgcacccttc agttacttca atatcgtcag ttcgaatcga cccatcttat ccttggcgat
    11161 ccagagaaaa caagatgcag taaaagacac agcaaagaat attcttgaga caagagaagc
    11221 tgttatccat atccttgatg aggacaacgt agaagaagta aataaaacag cagccaatct
    11281 gcctaatgag gaaagcgaac tgtacctgac tgacttgact ttaactgatt cagtaatcat
    11341 ttcagtacca gcattgacag aagcaaaagt tcgttttgaa acatccttat atgaacatgt
    11401 cgagatacgg aataaaaata aagtaacagc tgatttattt ttgctagaag tacagaaata
    11461 tcatttaaat gaaacagtct atgatgataa gacaggaagg atcgatccaa gaaaattgaa
    11521 tgctgtaagc cgtttggcag gaaatgatta tgcagcaata ggagaaattt tcacgattgc
    11581 acgtcccgaa taaaaaggag cactagttat gttaaaaata agctataaac catcgacaga
    11641 ttcaaaagaa atgaaaaaag aatatgaaac agtgaatgat tttctgcaag ggcagtactt
    11701 agaagttccg ccattgcaag accattttgt cgttactact gttactttag atggtaagga
    11761 aatagaaatg ccagatcaaa cgattagcgg attgtttaat tactttaaca aataaatatt
    11821 tattccgaaa agtgaaaaaa catataaaaa aacacataaa actaaaaaat tatcaaagaa
    11881 tcctggtgca actgaaagaa tttgcaacgg gattctttca acttatttgt tatttttttc
    11941 taaaaaatat taaagattgt taaatttcac tatttcattt caaattccat tgcctaatca
    12001 ttaggaaaag tctatcatga actcgtaaat agaatatagt aataaccttg ttaggtgagg
    12061 ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag
    12121 aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg
    12181 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
    12241 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
    12301 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggaggtcgct
    12361 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
    12421 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
    12481 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
    12541 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
    12601 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
    12661 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
    12721 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
    12781 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
    12841 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
    12901 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
    12961 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
    13021 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
    13081 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
    13141 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
    13201 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
    13261 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
    13321 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
    13381 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
    13441 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
    13501 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
    13561 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
    13621 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
    13681 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
    13741 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
    13801 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
    13861 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
    13921 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
    13981 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaata gcttttgaca
    14041 gtctaggagg aaaagctgca tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
    14101 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
    14161 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
    14221 atgctcctgg atctacgttt aaaatgatta cagcagcaat cggtctcgac aacggcacta
    14281 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
    14341 gatcttttaa aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
    14401 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
    14461 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
    14521 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
    14581 gatatggaca gggcgaactt ctaattactc ctatccagca agcagcaatg tattctgttt
    14641 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
    14701 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
    14761 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
    14821 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
    14881 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
    14941 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
    15001 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
    15061 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
    15121 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
    15181 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
    15241 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
    15301 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
    15361 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
    15421 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
    15481 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
    15541 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
    15601 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
    15661 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
    15721 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
    15781 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
    15841 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
    15901 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
    15961 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
    16021 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
    16081 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
    16141 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
    16201 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
    16261 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
    16321 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
    16381 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
    16441 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
    16501 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
    16561 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
    16621 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
    16681 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
    16741 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
    16801 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
    16861 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
    16921 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
    16981 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
    17041 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
    17101 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
    17161 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
    17221 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
    17281 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
    17341 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
    17401 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
    17461 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
    17521 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
    17581 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
    17641 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
    17701 tcactatttc atttcttgga ttcatttcga tcgttctatc tagccaatcc gtattaggga
    17761 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
    17821 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
    17881 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
    17941 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
    18001 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
    18061 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
    18121 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
    18181 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
    18241 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
    18301 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
    18361 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
    18421 ctgcactact agctgctggt ccaccgccaa tcactaatac atcataaggt tctttttcaa
    18481 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
    18541 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
    18601 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
    18661 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
    18721 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
    18781 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
    18841 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
    18901 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
    18961 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
    19021 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
    19081 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
    19141 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
    19201 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
    19261 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
    19321 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
    19381 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
    19441 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
    19501 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
    19561 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
    19621 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
    19681 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
    19741 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
    19801 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
    19861 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
    19921 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
    19981 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
    20041 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
    20101 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
    20161 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
    20221 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
    20281 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
    20341 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
    20401 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
    20461 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
    20521 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
    20581 catagcgatt tttattgcat aaaattcact tgaatacaaa aactaatatg ttagtttagg
    20641 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
    20701 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
    20761 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
    20821 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
    20881 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
    20941 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
    21001 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
    21061 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
    21121 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
    21181 attcttgctg tgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
    21241 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
    21301 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
    21361 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
    21421 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
    21481 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
    21541 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
    21601 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
    21661 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
    21721 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
    21781 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
    21841 ccaaatattc ctaatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
    21901 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
    21961 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
    22021 ggaaaaccat tcatcccgag tccagaccca ctattgacag accttcagca taagctttcc
    22081 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
    22141 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    22201 gaaatgttca tcaagatggt agcaggacca aatgccgtac gaaaaacatt acaggattac
    22261 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    22321 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    22381 cattgatgga tataccagta ggagaggtat ggcctaggga gaggatcaaa gaactaaaag
    22441 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
    22501 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
    22561 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
    22621 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
    22681 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
    22741 aatttggatt gccgggatgg gaacctgagc gcttagctcc tatcaaatcc ttgcttgaag
    22801 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
    22861 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
    22921 actcgatttt tggattagct cgaatcgtaa agaatcgaga agatttagat tggttgtgtc
    22981 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
    23041 cagaaaatga tgtatatggg attttagcag aattttgcca aagagatcga atcccatttg
    23101 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccacataaat
    23161 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
    23221 acggatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
    23281 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
    23341 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
    23401 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
    23461 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
    23521 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
    23581 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
    23641 aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg
    23701 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
    23761 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
    23821 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
    23881 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaata
    23941 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
    24001 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
    24061 caggtataaa caaatcaaat acagtacaaa gggactccac catttctctt gaataaagaa
    24121 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
    24181 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
    24241 ttggcagatt aggtcgcaaa atatctacag agagatcaat aaaaaagtca atatattcat
    24301 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
    24361 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
    24421 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
    24481 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
    24541 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtactatg
    24601 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
    24661 gctgactgaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
    24721 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
    24781 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
    24841 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
    24901 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
    24961 gaaaaatatc taattttgta ttgctttttt tcttcaagat aaacgtttta atcgcttctt
    25021 gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt
    25081 ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta
    25141 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
    25201 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
    25261 gacagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
    25321 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
    25381 catcaaagca aaaattgact attcgggaga cttagcacct atactacaat cagctgttac
    25441 atttgagaat gttgatccca ctttgtcgat tggctatgac gagaacactt tgactatcca
    25501 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
    25561 gctttttact gtcaaggtat cagatggact accgaacgat catcatttaa cgccgtatag
    25621 tcagcggcag acaattgagc aagattcagc catcgactca ggaggagatc ttgttgaaga
    25681 accaacagat aaaccagaaa atgaaaataa gccagaagta ccgcctacag aaaatccaga
    25741 cggagaacag aaaccggaaa tagaacctgg agaagaaccc gatacagaaa ctcaaccaga
    25801 gccagacaat aaatcaaaac ctgaaataac ccctggagaa aaaccagatg taactccaga
    25861 accagatacg gattctggaa accaaacagt accggaaaca aatccagaca cagataatga
    25921 aactgaaaat ccagaaaaac cagaggtaga tccagaagaa aaaccagatg tgactccaga
    25981 accagatacg gacgcaagag accaaggaat accagaaaaa ataaacaaaa aaacaattca
    26041 agaggatgga aaaaaagaga gtaaaaaatc aaatctagca attctaaaaa taaatgaaga
    26101 gcagctgaat aaaaaatcac gaatatttga tagcgcccaa tcagctgaaa ccttaaaaag
    26161 tagcaaggat acaacctttg ctgtcctgaa acgaaaaata aacagctacc aaaatccgga
    26221 gagtcacaaa acaaagttat actgtggagc ggaatcattc tattaagtat agcaacaatg
    26281 ctttctgcaa aaagatttaa acaaaacaga tctctctaac atttgaataa tgtctgtgaa
    26341 cgcatttttc aaataaaaag aaatatccag aaaatgaccc caaaatacca gattgaaatc
    26401 tgatatttgg ggtcgttcat actttatctt tcttcatatt tattttaagg gatatctgtt
    26461 tcttttggaa gatggttcgg gatctttttc actttcaata catctaaaaa gaaggtaaag
    26521 atcggaatgc cgacaatcaa gccccaaacg ccaaagaacc gttcgctaac aagtaagacc
    26581 acaaatgtat aaaaaatcgg cagttctgtt ttgctggaca tcaacttcgg attcagcaca
    26641 taagactcaa ttagatggac aatcaaaatc gtaatcagga tataaacaac atctcggatg
    26701 cctccttggg aataagctat gaaagataga ggaatacaag aaatgataac acctgctact
    26761 gggatcagac ttaaaatgaa aatcatgatc gctaaactca gcagctgatg aaagccaaat
    26821 acagccaagg cgattgtagt gagcaacgta tttaaaagag cgacaacaaa ttgagcttct
    26881 aataccaaac caaatgtatt gacaaactta tcggcaaaat agtaaatatc ttgaaagaac
    26941 caagaaaatt cactttttaa aaacaatctt gagaatacaa ttgttttttt cttttcaatc
    27001 ataaaaaaga aacttaagat gaatgacata gcaaacgcca ctgccatttt tcctatatcc
    27061 tgtaaatagc caagcgcaat cgaagcgcca ttttgaagct gttcaaaaaa atcgtttcgc
    27121 tcaatataag tatggatata ttgtaaaaca ggatctgcat cttttggctg atgctcgtaa
    27181 aaattaataa cggactggat catgtcaaat gtttgatgaa taagcatcgg gatatacttt
    27241 gtgaatgcca gatagataag gtaaacgatc agtgtataaa tcatgatagc taaaacacct
    27301 gtagggattc ggacaaatcg ctgaatgatt tgaacaagat gaatcatcaa ataagtaaag
    27361 ataaatgtga gcaaaatggt tgtaatcata cttctaacta agaaaagaac aaaaacgaca
    27421 aataataaaa cagtgagacg gcgtattttt tcattcgcca aaaaacgctg ataaacggtc
    27481 aaatcgacaa cctccaaata acaaaaaata aacacgttat tcattatatg atggaatatg
    27541 aaaataagaa agagcaaaaa gaaaaaccgc taaatgacgt tatcttatga gttggttttc
    27601 ctcataaaat cgttcatcta gcagtttcag ccctttttct ggtcttgatg aaaattatct
    27661 agcatttttc gggtcagatg ttgcagtgta tccaattctt cctgttcaag tggaagataa
    27721 gacttgatct tttgtggaat accttctgct tgtttttgca acgcagcacc ttgagcagtc
    27781 agcgtaacat ttacgattct ttcatcaccc gtttcacgct gacgattaat ataatttttt
    27841 tctgctaact tttttagtaa gggagtcagc gtaccagaat ccagacacaa atcatttcct
    27901 agctctttca ctgaagtctt tttcttttcc cataaaacta acatcacaag atactgggta
    27961 taggtaagat tcagcggctt gaggagggga gtgtagtgtc taatgatttc tttagaggcg
    28021 gcatataaag gaaaacatac ttgattaact aaatatagat cttccatttt tccacctccg
    28081 ctgttttact atagtgtatc atagcggatt tacacaaaaa agtctattca ctaactgctt
    28141 tcagagtaac ttctatattt ccagctgtag cttttgaata cggacacact ttatgcgctg
    28201 cctctaataa ttcttgcgtt tcttccagag aaagaccttc gatagatcct tcaatctcta
    28261 cacctaagac aaagctcgtt tcactttctg aataaaggga aacttgtgca ctgacagtag
    28321 aagccccaga gatatttttt tgctgtttga tcaattctaa tgcactgtta aaacatgcac
    28381 tgaaaccagc tgcaaacaat tgttcagggt tggttgcatt ttcgacgcgt ttaccaggag
    28441 ctgcaatatt caattcgaat gtacgatcgg gattatgcac ttctccagag cgtccgcctg
    28501 tgttgatcat tttcgtagaa tacatttttt tcataaagtt accaccttta attatatttt
    28561 atacaattga attttacaca atataattgt ggaaagcaaa gaaatggatt ggcataagat
    28621 aaataagaga aagcacatga aacagacgcg ctttctcgaa tagcaaacat atacaaaaaa
    28681 tatcagagac aaaaaataat ctcatcataa acattacata tatttctcaa aaagttgttt
    28741 tctttcttca aaaattactt cacgatggac ctgatgaagc atccataaat aaccaaaagg
    28801 atctctgaac attgagttag tcacaccaaa gtccatcatt tctgtcattt cttgaatctc
    28861 tacacagcca tttcgtaatg ctttattatg tgtgtcctcg atatcagaga cgatgatatt
    28921 gaaccagatc gtatttggat gttcagcagt aggagcagtc aaaccaagag acgtgttttc
    28981 atccagcata tggaatctcg taccatacaa ggtaaaaatg acttcattat tgcctctagg
    29041 gtaatctgtt gcctcaatca gttgtatttc atcaaagatt gtttgataca gttctagtgc
    29101 tttcaaacta tcatttaata caaaatcgat ttccgttcct ttatgcataa agatctcctc
    29161 tccttttcag tcgaacagtt actagattct tttattatac cagctaaaga aaacgcattc
    29221 aaaaaaatag cggatttact taccaactca ttcgattgga tgataagtaa atccgcgtcc
    29281 cattacttct gctacgctgc tgacaatgac aaaggcactt ggatcgattt cttctatcaa
    29341 tggtttcatt cgggaaaatt catgatcagt cacaacgatc aataaaacct cttttgactt
    29401 tcgattatac ccgccttttg cctctagcag ggtaaggcca cgtccaatct cagcacttaa
    29461 gcgaaggcgg atgtcttcca aataatgttc actcatgacc ataacagatt tctttcgatt
    29521 gatacctgtt tcgatataag tcatgacaat tgaagtgatt gcaatagata aaatagcata
    29581 aagaaactct tctattccaa aaataaacag attaagagaa acaacgaatg cgtctgtaaa
    29641 aaataagcca atcgaagtat tcaagtggaa atatttttta aaaatgaggg gagggatcgt
    29701 cgtaccgccg ctagaagagt tgtttttata taaaatcgca actcctaaag caaaaatcgc
    29761 acttccaaaa atgacagaca acaaccgatc tgaagtaatc attgtttctg gaactattgc
    29821 aatgattaaa ggaaaaacca agcttccgaa aagtacttta aaaaagggtt ttttacctaa
    29881 aaatagaaga gctagtacca acataacgat attcaacact aaaataacta tagctcggtc
    29941 aaagcctaaa gcagcttcca gtaatattcc tagaccgctg acacctccag cagcaatatg
    30001 atgaggccct aaaaacatat tgatagcgac tcctaatatg aagagggcaa aaataatctt
    30061 aatgtatgtc catataaatt gtctcgtttc tttcatttat tcacttcctt aattcattaa
    30121 taatactctt atcttattat aacttacttt tcaccttaag ttaactttaa ttaataaaaa
    30181 aattaggcta gtctaaatta ttatgtttca ttcttctaga atatacttct acgccaaaaa
    30241 ctgttaaaaa acttttactt tagaaaattg gagaaattga tcgcttaaga taaattcagg
    30301 acactaaata gtacaggaag tagactaaaa cttttaaaag ttttaagtct atctatttgg
    30361 caagtttaaa aaataaaaat tcggtatcaa aaaactgtta ccgataacat ttttgaaagc
    30421 gttgtctttt gcttctctat cacttatagt gatgacgtca tattaaaaca caaggaggaa
    30481 atcaatggat acagctgcaa tttatcatcg accggaaagt gaatatgcat ttctatatac
    30541 gaatacaaga tttcgtattc ggcttcgtac taggaaaaat gatatcaaaa aagtatatgt
    30601 gctatgtgga gatccatata ccataacaac tgaaaaatgg tatcaaaaac aacgaccaat
    30661 gaaaaaatgt ctaagtacca atgttcatga ttattgggaa attgaagtga cgagcgaaac
    30721 aagtcgctta cagtatgcgt tccatgttgt tggggaagat aacacggact gtttttacgg
    30781 tgatcaagga atttttcctt atcgagaaaa tgtgattact gaaccaaatt tttattttcg
    30841 aataccttat tttcatcaaa tcgatcgatt cactattcca gaatgggtaa aagaaacagt
    30901 atggtatcaa atttttcccg aaagatttgc aaacggggac aaagcaaatg accccgaaca
    30961 cactttacct tggggaagta aagatccaga ccgagaagac tttttcgggg gagatttgca
    31021 aggtgtgatg gatcatttgg attacttgac ggaccttggc atcaatggca tctatttttg
    31081 tcccatattt aaagccacat ccaatcataa atacgatacg attgattatt atgaggtgga
    31141 tcccgctttt ggcgataaaa aacttttaaa aaacttgatt gatgaagcac ataaaagagg
    31201 gatacgtatc atgttcgatg ctgtattcaa tcatatgggt gtccattcac cacagtggca
    31261 agacgttctc aaaaacggag aaaaatcgat ctataaagac tggttccaca tccgtttttt
    31321 cccagtagat tcttatcaga tgactgactt accagaaact gctgaaaata tcccatatga
    31381 tacttttgct tttactccat tcatgccgaa actaaataca gcaaatccag aagtgcaaaa
    31441 atatttgctg gatatcgcaa cttattggat aaaagaattc gatattgatg gatggcgtct
    31501 tgatgttgct aatgaagtcg atcatcattt ttggaaaaaa ttcagagaag cagttacaga
    31561 aatcaaacca gacatctata tccttggaga gatttggcat tcctctcaag cttggctgca
    31621 aggggatgaa ttccatgcag tgatgaatta tgcatttaca gattcgatta aagattattt
    31681 tgcaaaaaag aaaatcacag cttcacaaat ggtcagtgga atgaatcatc agcaaatgct
    31741 ttacagagat caggtgaacg aaggaacatt caacttatta gattctcatg atactgccag
    31801 gatcttgacc ctttgccaag gtaacaaaga attgatgaag tctgtgatgg cctttatgtt
    31861 tcttcaaaaa gggtctcctt gtatctatta cgggacagaa atagggatga ctggtgaaga
    31921 tgacccggat tgccgcaaat gtatgatatg ggagaaggag gaacaagatc tagagctatt
    31981 cggttttata aaagaacttg tttctctaag gaaacagttg tctaagatta tttctgaagg
    32041 ttctactcag tggctaatag taaatgatcg tgaagataag ctctatttca cacggaaact
    32101 agagggacag atcatctatg tttactttaa tcagtcaaaa gaaccttggg tagtagaaca
    32161 ggaaaatgag gtgattctta gtcaaaattg tcagttgtta gaagacggga aagcagagat
    32221 acgacaatac ggatttttga tcatgtgttg aaaactgtta ccggtaacaa caagaaagcg
    32281 tttgcaaatg aagatttgtg cccattataa taagggtgta acagcacgtt gaatacaaag
    32341 gaggaaatga agatgaaaaa aagaagtgtg aaacagttgg gtttagggtt aatctcttta
    32401 ggaatcttag gcggattagc ggcttgtggc aatggtaact cttcagcaga aggggaagcc
    32461 gatgaaggaa aaacgttaac agtttccgtt gattctggat ataaagatta tataaacgaa
    32521 ataaaaaaca ctttcgaaaa agaaaacgat gtgaaaatca agctagtaga aaaggacatg
    32581 ttcgatcagt tggaatcatt agcgttagat gggcctgctg gaaagggacc tgacgttatg
    32641 atggcagcat atgaccggat cggtgcgtta ggacagcaag gacatctggc ggaagtaaaa
    32701 ctgggaaatg aagcagctta cgatgaaaca gacaaagccc aagtgactta tgatggaaaa
    32761 atctacggag aacctgcagt aatcgagacc cttgttttgt actataacaa agatttagtc
    32821 gatacagcac catcaacgtt taaagatttg gagaatctat ccaaagacag tcgttttgct
    32881 tttgagtcag aagcaggaaa aaatacagga tttttagcta aatggactga cttttattat
    32941 tcctatggtt taattgcagg ttacggtggc tatgtatttg gtgatgatgg cacaaatcct
    33001 tcagatatcg gtttgaataa tgcaggtgct gtagaaggaa tctcttatgc aacagattgg
    33061 ttcaaaaatg tttggccaaa gggaatgcaa gacataaaaa gtgcaggaga cttcgccagc
    33121 cagcaattca tgtccaataa aactgcagca atcattgacg gtccatggca agcgcaaaca
    33181 tacaaagaaa acaatatcaa ttatggtgta gccaaaattc ctactctaaa taatggacag
    33241 ccttatcaac cgtttggcgg aggaaaagga tgggtagtca gcaattatgc taaaaacaaa
    33301 gacctctctc aaaaatggct tgattatgta acgaatcagg aaaatcaaga aaaattcttt
    33361 gaaatggtaa atgaaatccc tgcaaaccaa caagctaggg aaacagcaaa ggctaaaaac
    33421 gatgaattaa caacagctgt gattgaacaa tatgaaacag ctcaagcgat gccaaacatc
    33481 ccagaaatgg gcgaagtttg gacaggtgca gaaaacttga tgttcgatgc agcatcaggt
    33541 tcaaaaacac caaaagaatc cgcagatgaa gcagtaaaaa caatcagtga agccatagaa
    33601 caaaaatact gattaaaatg ataaccgtcc atttttataa aaaatggaac aggtgcttca
    33661 gataaaagtg gcaagagcga gatggaaaat tatcttgctc ttgttgccat tatcttaaaa
    33721 gcagtttgcg gtttagcaag tttagagggg gcattcagat gcagcaagat aaaacaagca
    33781 atatcaacga aaaaaaaatc acgctttgtt cacttattcc agggatgggg cagtttgtaa
    33841 atggccaagc ttttaaaggt atcgtttttt tcgccatatt cgtcctattc attggtcaga
    33901 tgcttctggg cggatttcat gcattggtag gattggttac tttgggaagt ataccaatgg
    33961 aagatcattc attatttatt ttgattgaag gcactttgca attcatcatt acaatcattt
    34021 tcttgatttt ttacggtttg aatcttttag acgcttatcg agtagcgaag atgcgtcaag
    34081 agggcaaaaa aatcaatcgg acgatgaagg aagtcataca taatataggt gatgaaggtt
    34141 ttccttatct attgacactt cccgcttatc ttctcatgat ttttacgatc attttcccag
    34201 tcctagttac attgttcact gcatttacta attatgactt caatcatatt ccaccagcca
    34261 gtttaattga ttgggtaggg gctaaaaact tttttagtat cttcttttta agctcatatc
    34321 gtaatacttt ttttgcagtc ttttcgtgga ccgtgatttg gactttatgt gcaacaactt
    34381 tgcaaatcac ccttggtatt ctcacagcgg ttgttgctca ccagaaattt attaaattca
    34441 aacgtatttt cggtatcatt ttcttattac catgggcggt cccagctttt attactatca
    34501 tgagtttttc gaatatattc aacgatagcg taggtgccat caatactcaa gtgattcctt
    34561 tcataaacca gcttcctttt gtagatatca gtgcaatctc ttggaaaacg gacccgaact
    34621 ggacgaaagt agcaattatc ttagtgcagg gctggttagg atttccctat atctatgtga
    34681 tggttacagg aatcttacaa tcgatttcag aagacttata tgaagcagca aaaatcgacg
    34741 gagcaaatgc atggcagcgc ttttcaaaca tcacgcttcc tgctgtcttc ctagtggcag
    34801 cgccaacatt tattacacag tatacgggga atttcaataa tttctccatg atctatctgt
    34861 ttaacaatgg cggtcccgga agcgtaggcg gaggagctgg ttctactgat attcttattt
    34921 cctggatcta taaacttacg actggaactt ctccgcagta ttctatggct tcagcaatca
    34981 cgttgattat ctccgtcatc gttatctgtg tgtcattgct ggtatttaaa aagacaaatg
    35041 cgttcaatat gggggattaa caatgaaaaa atttcattcg agtgctcgat tccaaaaacg
    35101 actttctcac ttttttactt acttttttct tttaatactg tcagtcatta tcatctatcc
    35161 gcttttgatc acagcaagta ctgccttcaa gtcaggaaat attgcagctt ttagtttaga
    35221 tctttcaagt gactggactt taggaaactt tactcggcta ttcagtgaaa cactttacgg
    35281 gacttggtat aaaaatacat tgatcatttc tgtcctaaca atgatcgtac aagtgactgt
    35341 agtaactttg gcgggctata cttatagtcg ctatcgtttt atcggccgca aatcaagttt
    35401 gatgttcttt ttagtgattc agatggtacc gactatggca gcattgactg ccttttatgt
    35461 catggctttc ttattagaag cgctcgataa gtactggttc ttgacaatga tctatatagg
    35521 cggtggaatt ccaatgaata cttggctgat gaaaggatac tttgacactg taccaataga
    35581 tttagatgaa tctgctaaac ttgatggggc agggcatttc aggatttttt tccagattgt
    35641 attaccgctt gtcaaaccga tgctcgccgt tcaagcatta tgggctttta tgggaccttt
    35701 tggggatttc atgttagcta agttcctgct tcgaacacct gaaaatcaga ctgttgctgt
    35761 cggcttacaa acatttattt ctgatgtgac aaaccaaaga gtctctcttt ttgctgctgg
    35821 ggcaattttg attgcactac cgatttgtct attattcttc tttttacaaa agaactttgt
    35881 taccggcctg acatctggcg gaacaaaagg ataggagcag agtatcatgc aaacatttcc
    35941 gttaaactat ttttccgcat tacgtacgcc aactcaagtg tttgcgggac gcaaactatt
    36001 atcatggcct aaatttttct tgatatttgt atttttagtt tccttgatgg tcatgcctgt
    36061 gactcttttt tatgccaatc agatccaagc gattccatta gaacaatttc tatccgttca
    36121 ttcgctgatc gatgaacaag gaacccaaaa attttctgaa cttgaattat ctgaaactgg
    36181 gttacaagca tcgcaacaaa cgatagctgt gacccctgaa attttggtag gtgtgtcttt
    36241 atctgagaaa caacaatccg atcatggaac ttttattgat tttgagaagg aacaatgggt
    36301 gatccaacaa aaggataaat ctggtatacg ccgctatacg atgaattata gcccatcttt
    36361 ccaaccagat tctgtacgta cgcccgaaga tttccaacga tttttagaaa gagaatttta
    36421 tgccagtaat cgtccaacaa tcattttatc ttatagttta agtcttggtc ttgtcctatt
    36481 tgtcatgacg agtctcattt tattcggggc ttcctttttc ttgtggatga caagaaaaag
    36541 ccaactttca tccattcata cattcaaaga gagtgcaaat ttgatgctga atgttatggg
    36601 aataggttcc atggcagcag cagttgtagg gtttatccac tttgacttta tcttgatgct
    36661 aagtgtccaa acaacgataa ctgtcttgct gcttttatgg gtttttgtca aaacaagatt
    36721 caaagataag cgtgtcgaat gacaagttta agcgatttat gcgacaatga atgtgaaaaa
    36781 aatgaaaaat aaagtagttt ttgaaaaatt tctgaaaaaa ttaaacttct tagttgattc
    36841 ttttcttttt tagtattaac gtaatagggt agttttttta aggaggaaaa tcatgttaga
    36901 gaaaacttta tacaatcaat tattttcacg ttctttctcg ctccctgtcg aagtaaatta
    36961 ttgggacggt acaacgaaac aatacggaga tacggacact cctccccaaa tcaaaatcaa
    37021 tatccatgaa gaaatcccaa tgaaagagat tacaagtaac gcttctttgg cactaggaga
    37081 agcctatatg gatcatcgaa tcgagatcga gggaagtatc caagcattga tcaacgatgc
    37141 ttatcagcaa gcaaacagct ttttacgagg aaaagattat ctaaaatggc tgccaaagaa
    37201 agaaaaacat acgaaacaaa aaaacaaaga agatatccat gcgcattatg acttaggtaa
    37261 cgacttttat aaactttggc tagatccaac tttgacttat tcttgtgctt atttcaatac
    37321 acctgaagat acgttggaac aagcacaaat caataaagtc catcatatct tagacaaact
    37381 ctttatcaaa gaaggcgaaa cgttgcttga tatcggttgt ggctggggaa cattgatgtt
    37441 cactgctacc aaagagtata atgtcaaggc aactgggatc acactaagtg aagaacaata
    37501 cgatttgatt accaaaagaa taaaagaaga acatttagaa gataaatgcc gtgtattatt
    37561 aatggattac cgcgaactaa aaggcgaaac gtttgatcat atcacaagtg taggtatgtt
    37621 cgaacatgta ggttctgaaa atcttgaagg ttatttcaaa gtggtaaaag atttattgaa
    37681 accaaaagga acagctttga tccatggaat cagccgacaa caaggcggtg cgacaaatgc
    37741 ttggatcaat aaatatattt tccctggcgg ttatgtccct ggtgttactg aattagttga
    37801 taatattaca aaaaacgatt tgcaactgat tgatttggaa agcttacgtc gtgactacca
    37861 attgacatta gaacattgga caaagaattt tcatcaagtc caagatcaag tcacgaaaga
    37921 aatgggtgat tccttctatc gaatgtggga tctatattta caagcttgtg cagcttcatt
    37981 tgaatcaagt aatatcgatg tgattcaata tttgcttgtt caacctggaa acaatgacat
    38041 cccaatgcat cgtgcataat tgaggcaaaa gtactgttta aagactattt aaaaagaagc
    38101 taagttttat gatgctccag aagtgaactt ttttgtctaa cttctaggat tgtctctaaa
    38161 gcttagtttc tttttcttta tattttcata ataaaagttt ggttctttaa tttttaaaca
    38221 tgacagaaaa ttttttcttc gttatacttg taatagaata ctattttata acaaagggga
    38281 tcgaatcaat gaaaaaaact gttatttata ctagtttatt tgcagctttg tttttagctg
    38341 cttgtcaatc aaataccaat caaggcacat ctgggaatac gtcttccagt agcgaacagc
    38401 gctcttcaca ggtatcggac tcctcaacaa aagcaagcac atcttcgtct agccagacaa
    38461 aaagttctag tgaagcaagt actcagtcag atatcaaagt gtctgcagct cgtgcgatcg
    38521 aattgttcca agataaatac ccagatactg caattactag tttggaatta gatagtgatt
    38581 ggggatctta cttctacaag atagaaggtg tcgatgatca aaaagaatac gaagcaagaa
    38641 ttgatgctca aaatgaaaaa atggagacag agaatccaga aactctagat cgagatgaac
    38701 aaaatggtca aaagaaaaaa gaagatggtc tggatgttga taaattgatc acgattgagg
    38761 aagcagcaaa aatcgccgtc gacaaagtag gaagcggtac ggcaacagat tgggatttgg
    38821 ataaagattt aggtactact tattgggaag tcaaagtaaa agacggcaat caaagtacat
    38881 cagtcaagat caatagccaa acaggcgaag tactgtcaac tgaacaagat gattaagaat
    38941 ttcttcggaa aacaagagtt gatttttctt ttagtcatga tacactagac aaaataactt
    39001 gtttgaagga gcgatttact tgagtttaga aacgaccttt gccaatcata tctttgccaa
    39061 tcctttgatg aatgcatctg gtgtccactg catgactacc caggaattag atgaactggc
    39121 tcattcagaa gcaggagcat ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa
    39181 tcccgaacca cgctattttg atgttccttt agggagtatc aattctatgg gacttccaaa
    39241 cttaggtttt tcttattatc tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca
    39301 aaatcaaccg ttattttttt ccattgccgg gatgagtgtc caggaaaact tggaaatgct
    39361 tgagaaaata gaaaaaagtg ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa
    39421 tgtgcctgga aaaccacaac tagcttacga ttttgaggcc acttatgaaa cactgaaaga
    39481 agtattcagc atcttttcta agccgctagg aatcaaattg cctccttatt ttgattttgc
    39541 tcattttgat cagatggctg atatcttgaa tcagtttcca ttgacctatg tcaatgctat
    39601 caacagtgtg ggcaatggtt tgtatatcga tacagaacaa gaagcagtag tcatcaaacc
    39661 aaaagaaggg ttcggcggca ttggcggaga gtatatcaag ccgactgctt tggcgaatgt
    39721 tcgcgctttt tatacacgtt tgaaaccaga aatccaaatc attggtacag gtgggattcg
    39781 gacaggacaa gatgcatttg aacatctgct atgtggcgca agcatgttac agattggtac
    39841 cgagcttcat aaagaagggc cggaaatttt ttctagaatc ataaaagaat taacgcagat
    39901 tatgtcagaa aaaggatata cgtcaattga cgaatttaaa ggcaaattac gcaccatttc
    39961 atagcaaaga gacgaaggtc atgattgatc ttcgtctttt tttataaaaa acactcgtct
    40021 gttcattcct gtgcaagcgt actcattgta tgttagacga acttgtggga aaataacaga
    40081 ggcaaaaagg aggataatat gaatgatgca acagaattag caaaagagct taaagaaaaa
    40141 gaatttagtt ttcaagaact agttacggat atacagaaaa aaataaagaa gcaaaatcat
    40201 gaattgaatg cgtttgtcgc gcttgaacca ttcgagagta attcccacga gctattgatt
    40261 ccccagtcag aaaaagggcc attggctggt atcccatttc ctttaaaaat gctcggtcaa
    40321 gaaaaaaagg gatggctagc tacttcagga tctcgtttat ttgaaacaca ccgagcttct
    40381 catacatcta attatgtgcg gcaagcagaa gcgataggtc tagttccttt tggacaaacc
    40441 aatgcgccag agtttggttt caaaaatatt acagacccag tcatctatgg cccagcaaga
    40501 aacccttgga accttgaatg tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt
    40561 tcatcaggaa tcgttccttt agctggtgct agtgatggcg gtggttctat tagaataccc
    40621 gcttcgtttt gcggattgat tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca
    40681 tatgcttgga gaggatggca aggagctgct attgatttcg gcttgacagt ttctatgaga
    40741 gacacggagg ctttatttta tgggatgaga agtatccatt ccggtgcgcc atatcaagta
    40801 tcgccagttg agtggcaaac acatccaaga aaaaaacggt tgaagatagc tttttgtgtt
    40861 gattcaccga tccactcaca gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta
    40921 gatttccttg gaaaagaagg gcatgatttg acagagattc cttacccact agatggtcgt
    40981 aaactgatca cgtcttatta tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg
    41041 attgaaacag gcatccagcg agctgtcaga aaagaagaac ttgaacccat ttcttggact
    41101 ttgttccaat atggtcagaa gctctctgcc gctacttaca tcgattctct gcatgtatgg
    41161 gatgaagcag caattgtcat ggaaaaccta catcaagaat acgatctatt cctttcacca
    41221 acgactgcat ttaccgcccc taaaatcagc gaagatttgc aaagtgatgc gataagagca
    41281 cagatggaac acatcagtga ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg
    41341 tttgaggaga gcttgaagat cacaccttat acacagttgg ctaatctgac tggacaacca
    41401 gcaatcagct tgcctactca tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg
    41461 gctgccagag gaagagaaga tttgctattt caaatcggct atctatttga agcaaatcat
    41521 cagttccatt taccaaatcc atggaaatag cagaaacaag gagaagaaga atgaagaaaa
    41581 aaagattgtt tgctctaata actagtgcct tttttatttt aagcgcttgc ggtacagctg
    41641 ccgctaatca ggaagataac agctggagta atgtagaaaa aaagaaagaa ttagtagtag
    41701 cgacttctgg cacactgttt cccgcatctt attataatga tcaaaatgaa ttagttggtt
    41761 atgatgtaga tttggcaaaa gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag
    41821 aatacaacgt ggatggacaa atcacttctg tttcaaaaag agaatcagat tttgctgcga
    41881 acgattttgg tatcacaaaa gaaagagaaa aaaaattctc cctttcagag actttcaaat
    41941 attcatttga cagtatgatc gtacgaaaaa aagatgattc aggaatccac tcacttgaag
    42001 atcttaaagg gaaaaaagca gctggcgaac ccaatactag ctatatgcgt ttagcggaaa
    42061 aacacggagc taaattagtc acatatgaca atgccacgaa tgatcagtat ttaagcgatg
    42121 tagcaaatgg tcgaacagat gttattctaa atgattatta tctgcagaaa atggccgtat
    42181 ctgctttacc agatatccct gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa
    42241 gtggtttttt attgaaagaa ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa
    42301 aagagatgaa agaagatggg acgacaaaga aaatatctga aaaatatttt caaactgatg
    42361 tttcagtcaa accgaaagaa acgatggctg agtcggtgag tgagtgatgt actttcctct
    42421 aattgatttt tccctcatgt ggacttcact tcctttcgta ctgcaaggct tggtatacac
    42481 attagggatt ggcttcgtgt cgtttctttt aggaaatctg gtagggttgc ttttgacagt
    42541 tttaggtctt ttggattggc tgcctctgaa tgtatttatt cgtttttatt tatcgttctt
    42601 tagaggcatt cccgcactag tcctgctgtt tctgttatat tttgggttac cttatcaatt
    42661 aagtgcctta acggcttcag tcatctgctt tacgattacc agcagtgcat ttattggtga
    42721 aatctatcga ggatcactag caggcgtttc ttcaggtcag tgggacgctg gtttagcttc
    42781 tggtttttct ttctttgggg tattgaaata tatcgttttg ccgcaagctt ttcgaatctc
    42841 agtaccggca ctcagtaacg tagctatgga tctagtgaaa ggtacttcgt tagcagcaac
    42901 aatcacagtg ccagaaatat ttcaaaaagc aaagatcata ggcggtcgga cattcgatta
    42961 tttttctctc tatattttag ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa
    43021 agtgcaacag tatttagaaa aacgttctat gcatcagtca atctgaaaaa tagaaatggt
    43081 gctagcgtgg ctggcaccat ttctattttt tgtttcttgt atattctgcc tagtatttta
    43141 aaaccatttc atgtaaaatg taaatcgaaa atatgttcgg aggtagaaaa catgaagacc
    43201 ccgctaacca tcgaaataga atcctcgtta taccaatatt gcatcgagca aggcggcttg
    43261 gttgtagaag aagtcacgat gcctgatgac caaggaattg tagatacgct tagctgcttg
    43321 acaaaatttg atggaagccg agaatggcga tgctatgagc tgaaagtgac aaaagcagat
    43381 tttcgctcta cagccaaact gtcatttgtt ggacattata attattttgt tttaccaagt
    43441 aaactatatg aagaagtaaa aactgaaatc cctacaggaa taggtgtttt agtgtatcgt
    43501 ccgtacacgg ttgaagagga gatgcctgcc tctggtacat ttctgattgc gaaaaaagca
    43561 gcaaagaaag agcttgctgt accggaagaa gctttgacaa accgcttcat ggcttcttta
    43621 ttcagggaag taagaaaagc aaagcaaatg gagcgaggcg taaaatattt tccaacagat
    43681 caattgtaca aagaattgaa aagacgttca gaaaatcaag atccattttc taacgaacga
    43741 tattatgaac ggtttttaga ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa
    43801 gttatcgcgc ttcagcatga ttatgaatat ttgaggcaac aacagcagat ccgtcggttg
    43861 cctacagagc cattggagtg aaaaatatgt actatcctta tttaagagga aaacaatttg
    43921 acctattagc attaaaagaa gcactgtcgc gaggattgct ctccaataaa atccaaccag
    43981 tgatcgaacc agtacgtgat tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa
    44041 agcaccatcc aatcgctgtg atccaaaatc cccaagtagg acaattcaaa ttattcgatc
    44101 agcatgtgca cagttggaca gtcaaagaaa atagctctgt cgtccctgca cagatcttaa
    44161 cgccagaaaa tctcagtgaa gtactggatt cacctccagc gtttcttgtt ttcgatggac
    44221 agcactaccc aaaagataca gaggtgtgga aacagttggc aggagttgat tcgaaatttt
    44281 tgataccaga tactagtcgc tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg
    44341 actcttttca aacgagaaag catgttgaaa gttatgcaga taaaacagat gactttttta
    44401 gtgatgacta tctttttttc cacgcagacg gatatattgg attttctgat ttcacgatcg
    44461 aaggtagccg ttatttcgat aaagggttcc ctagccgagc gcttgctatc catctaacgt
    44521 atattgatgc ttacggcaat atacgcgtga aacattttgt ctctgacagt aacgactctg
    44581 ctaaagacca agcattgaag tttcttgaag caggagataa aatgaaaaaa tggatcatgc
    44641 gtcaccatca tcaactcttg attacttcag gaatgattga attattggct ctttaccagc
    44701 aacaaaaatt ccctggactg ggagtgttga aaaagtggtc acttatgcat catttggaac
    44761 ttattagtca cctgctagat catccaaaag attggcttaa atattattca aaagttcagg
    44821 aactatatga agtgatccaa tgattagata atgtaaatga aaaaggaata gaaaagagga
    44881 aactggatgt atattcggca aacaaggaaa gaagaagtag aagaagtggt agaaatcatt
    44941 aatgccgcag cggatttcat tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa
    45001 ccaacaaaag aaacgatttt gaacgatatt gaaagaaaag aaagctatgt attaatagtg
    45061 gaagatcaaa ttgctggaac agctgcatta gtctcaagcg tggatcccgt ttacacaaaa
    45121 atagatggta gttggaaaga atcaactggt ccttatttat cgattcatcg tgtcgcttta
    45181 gcagaaagta ctcgaggaaa aaaactagca aaaccgttct tagaacattt gttgacgatt
    45241 gcagccagca aaaatatcca agatatccgt atcgatactt atccagctaa tgaaccgatg
    45301 gaaaagacca tctattctgc tggattcact tatcaaggga tgattgaatt tccttttgaa
    45361 cacggcgaaa gaaaagcata tcaaataatc atcaattgac tataaaatca tctgagcaaa
    45421 gataaagaca ttaacactac tgaaaaaaac aatcatatga tagccaagag gttgtaacag
    45481 aagtggccag cttcaaaaaa caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg
    45541 aattttagcg tattttacga aatagctgct ttctgaaact cccttcatcg agaggtcgtg
    45601 acatagcttt tgtcacgacc tctcggctta tcctacgagt attattcgat acatttatat
    45661 caataatcct ttttttattt tatctgcagc ttcttggaac gctccatcgt ttgacacgac
    45721 taaaagtgag gggatagctt tcaaacgttt caactgtgct aaatcttcta cataaaccgc
    45781 cagtctggct gaaatctgtt cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt
    45841 ttctttagag atcgtaataa agataggacg gactaccgtc ccgaaatact catataaatg
    45901 ccaaaaacca tttgctgtca aaacggcgta acaatttcct actgtcagtt tctttttaat
    45961 ttctgcttta ctggaaccat atttatgttg agcatattgg tcccactctg caaactcatt
    46021 tcccttgatc atctgattga atgtattgtc agaaacaaaa taataatcct cttgatcttt
    46081 ttccccaggt cttggtgatc gattcgtgta agtaatgatc ttttgttccg gctgaaaaat
    46141 agcttctgcg agtgaagttt ttccagaacc agaaggaccg ataaatacat agcaataatt
    46201 cattgtcatt tctcctttga tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa
    46261 gcttttaaaa gtaactttgc ttttttgtat attataccag caagccagtc acaatagcac
    46321 ttaaaaagct gactaaagtc gctgttagca aaattttcaa tccgtgtttt gcgacaagat
    46381 ttcctttttc ttcatttaat cccttcatcg caccagaaat gatcccgata gaagagaagt
    46441 tggcaaatga aacgaggaag acagatataa ttgctgttgt acgctcagta aaatggaaat
    46501 taccatttgc taattctgtc atagcaacaa attcatttgt aaccagttta gtcgccatga
    46561 tactaccggc atccactgct tctttccatg ggattccgct gatgaaggca agaggggcaa
    46621 agatgtaacc taggatctct tggaaagtga tgccgaatat cccgtggaag atagcattga
    46681 tcatggcaat caaagcaaca aaaccgataa gcatagcagc aaccgtgatc gccacatgga
    46741 aaccatcaag aatgtattct cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa
    46801 tgatcagctc atcttctttt tcttctaata catagggatt tacaattgaa gccagaatat
    46861 aaccgccaaa taagttcaaa acaagggcag tgatcacata tttagaatcg atcagtgcca
    46921 tataagatcc gacgatcgac atagaaactg tagacatagc cgatacactc atggtgaaca
    46981 aacgtttctc agataggaag ggtaattcct tttttatgga aataaaaact tccgattgtc
    47041 ctaaaatagc agaagctact ccattataag attccaattt gcccatcccg ttgattttac
    47101 ttaatcctag accaacagct ttcataaata atggaagaat cttgatatag cgtagaatac
    47161 cgataatagc agaaataaat acaattggca tcaatacgga aagaaagaaa ggtgcacttc
    47221 ctttatttgc tacaccggca aaaacaaagt caacaccttg cccagcaaaa gcaagaagat
    47281 gatcaaacat ttttgccaat acagaaacaa ccgctgtacc aaaagttgta cgtaataata
    47341 cgaaaccaag aacaagctgt aaaacgaaca tcacaattag tggtttcaat ttgattgctt
    47401 ttttgttact gctgatcaaa aaagcaagcc ctaatgtgac aaataaacca ataataccta
    47461 ataaataact caaataatca ctcctattcc aaattccgtt ttattttacc tgttttgcaa
    47521 ataaaatact aatctttagt gaaattttca ttactttttt agagtgaaac gcattcattt
    47581 tccattagaa aataaagcga acaaaataaa aggtttaaaa agtgtaacgt cttagtaaag
    47641 tctataaaaa aacgaaacta tcgattaagc taatagtttc gttttcaaca tttataagta
    47701 aactttttct attggtaata tagattttcg gaagggtaga caggtgcgca ggtaatatcg
    47761 attcctagtt ttttcaatgt ttgttcatca tttttattga ccatcacagt agagtgggct
    47821 tgtgctccct caagttcagc tagtttatca taagcaactt gagcgattgg attcgttact
    47881 gcactgattg ataaagcgat caagacttca ttgatgctta gtgcatccaa agaggaatga
    47941 agtgtctttg atttcaatcc ttgtattgtt tcaagaacta atggtgacaa taaaggaatg
    48001 tcatctgcga tatgggctaa atgttttact gcatttaaga taactgccgc tgacgcatcc
    48061 atcaatgaac ttgtacgccc ggtaatgatg acttcatctg gtaattcgat tgcaatcaca
    48121 gcagaaggat cttttgtagg actttgcgct tgaatcttat ctgcatagtc atgggcaggg
    48181 actaccacgt ttcggtcttc aggacgtaaa ccgacttctt ccataatcag tttgattcga
    48241 ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc attctgtttg gaaataacga
    48301 cggatgattt cttgtttaga ggcttcttgg acaacggtat catccacgat cccaaatcct
    48361 acacgattca cacccatgtc agtaggagat tgataaacag attctttacc agtaattttt
    48421 tcaataatac gtttgattac aggaaatgtc tcaacatccc gattatagtt cacagctact
    48481 ttattgtagg catcaaagtg gaaggaatcg atcatattga catcttttag atcgactgtg
    48541 gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa cattccatac tgggaaagtt
    48601 tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt catgatacag ttggttcaag
    48661 gaagtagcga gctttccact tcctgggcca ggagctgtca ctacaacgat tggtttagtt
    48721 gtttcaatat actcattttt tccaaaacca tcttctgaga cgattttttc tacgttgact
    48781 ggatagtctt cgatttctga gtgtttatag accttgatgt ttcgtctttc tagtttattg
    48841 ataaagactt tcgtggcagg ctgtccacta tagcgagtga taacgacact gttgattaat
    48901 aagccataac cacgtaattc atcgattaac cgcaagatat ccatatcata agtaatccca
    48961 taatcaccac ggatcttatt acgttcaata tctccagcat agacgcaaat cagtatttca
    49021 gcttgatctt tcaatttttg cagcaatttg attttcgcat cttcttcaaa tcctggaagt
    49081 acacgtttcg catgcttgtc gtcaactagc ttcccaccaa attctaaata taatttgtca
    49141 tagtggttca ctcgttccaa aatatacgct gactgctctt caatatattt ttctgaatca
    49201 aaaccaattt ttttcactag gttccctcct taagtactaa agcatttttt catttttttg
    49261 aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca
    49321 acttgtctag atagttattc attggtatga tagtttttga taaggaaggt gctacatgaa
    49381 aaaaagacag aaaaagaaaa acgcatataa acactatatc cgttcgattt ttaccggtta
    49441 tgaaagaatg cttgaagatc ccgaattgga acaattgacg tttacttatc taaacgaaga
    49501 aacgcagcta actcgtgatg agcatcagcg cattcatttc acaacaagag atctgcctag
    49561 taaataaaaa ataagataaa ggtcatcgga ttcgattctg tcctatcaga tggaaagact
    49621 agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc
    49681 cgctcttttt tataacaatt ttttacagaa aatggtacta ttgaagtatt aataagtgaa
    49741 aaacagatga gtaagaggag gcaagtagta tgaaagagcc agatacaaat gcaaattatg
    49801 agccagacga actagaggag acgaatgcgg atcaggatac aggacaagaa tcgtcttctt
    49861 tttacgatat caaagaaaaa gcaagcatga atctttccca attattagaa gacttacagg
    49921 cttttgaaaa agctgtccgc gaagaagata tccctgagat ctaccgttta tacaatggcc
    49981 gattgaatga agaattaaaa agaagctcaa atgaaaatca cgagatcgat caattgctga
    50041 tgcgtaagat ccatgatcgt tttttacagg aatttccttt tatggaacaa gtagaaaaca
    50101 tctcgccaac gatgagctat tacaaaatcg gcacttatta ccatgatcgg cccacggtcg
    50161 gtattgatgc aagtctgcca gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga
    50221 aatattccca accggagtca acggactatg acaaaaaaat caatgagcta gacgcaaagg
    50281 tgatcactgc tcagacagaa attgaacgtt tagatgaaca aataaaagaa atcaatcgac
    50341 aaatgactga tttgaacgac aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa
    50401 ttcaagaatt agagaagaag aagcaagtac tatctaatga aaaattaggc tggcttcctt
    50461 atattgagac gcctgaaaca atccagcagc aaaaagaagc attgaagcaa gaagcaagag
    50521 cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg
    50581 gtagcaaaga gggattcggt caagcaatcc atgaattctt gatgaattat cttggcaatg
    50641 agaatcctaa taatcaatct aatgaaggag gttccgagta tgaatgaaca cgatgaagca
    50701 gaatggacaa aaaatgaaga acaaacagaa gatcatgaag aaacgaacac ttcattggaa
    50761 gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa
    50821 atacacgaaa aagaaaaagt aactccgacg aatacgaata ctgcagaaaa cacccatgct
    50881 tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag agcaagatca tcaagatatc
    50941 gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt tacgtatgag aaaagatttg
    51001 atcgaatcag attttccaca attattagaa tcatatgatc gttttatatt gaatgaagaa
    51061 ccaatctcca cgatcggaaa gacaagcctg atcgaatact tcacgtatga attactgaaa
    51121 ccgttgaatg atattggtct ggaacggtca aattcagctt atgacacatg gaaaacaaga
    51181 catttttcga tcaattatac gttgaatgga caaaacgaat tagtcttttc ctttgtatta
    51241 ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag
    51301 acaatggaaa tcaatgtcca agatgaccgg gtgctttctt tgatccgaca ctggtcagtg
    51361 gatcgagtat tctctgcagg gcagatcacg atattcaatc acaaattgaa tcaattatta
    51421 gcccatgcaa gaacacttgg ttttaccgtt aatcagacat tactagacaa tacgaagcca
    51481 cttcgtttga atcttcagag cgaatttgaa ttgacgaatg aagtattgga tgatattttt
    51541 atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga tcaacgaaac tacttatcaa
    51601 gtacgattag ataaaggaca gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc
    51661 ttgtcgatct catctggaga ctacccacga tctgtcattg atttctttat caattacgaa
    51721 ttcttggtcc cattaatggt gagaaagtcc tgattggata agttggatta ccagatgtca
    51781 ttcagataat tctgttccaa aggacttgcg gcagttatcc agaataattg tcacaagtct
    51841 tttctgtatg tactaaatgg acgtgaataa cttgctgatg aaggagggat ttcattagga
    51901 atgtccacaa ataaaatagc acagcttagc ttattgtctg ctgcttgtgt agcaggtagg
    51961 atcgcttttc aatttattcc gaatttccag ccaatgactg ctattttttt atttatagta
    52021 ctttacttaa gcttaaaaga tgcgttaatt gtcatgagtc tttctattgc gatcagcagt
    52081 ttttattttg gtgttggacc atgggtcatc ggccaatgga tcagctatac tgttgttctg
    52141 agtttgtttt caatcgtctg cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga
    52201 gtctgttttt ttctggcggg catggtttac ggaataggta tgaccgtttt tgatacgctg
    52261 ttatatcgtt tgcctcaacc ttggatctat taccttcaag gggtgtcgtt tgatttgatg
    52321 cacagtatag gcaatgttgt gttctttctt gtttttctgc cagtcgtcaa acgtttttac
    52381 aatcattcag gaggaaaaaa tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag
    52441 tatactcatt ttgacaagtt gcagtacaac ctatacacaa tcggcttcca accaaaagga
    52501 ggaagtaaaa acatcgcaaa aagcaacaat cacgttacaa gaaaatggtc aaaacttttc
    52561 ggaaaaagaa gtgttcttca aaaaaggcga tgatttactg agtattttga aacgaaattt
    52621 tgaagtcaaa gaagataacg gcttcatcac ttcgcttgaa gggcactcac aggatgagaa
    52681 aaatcaactc tattggatgt ttactgtgga cgggaaaagt gctactactg gtgccaaaga
    52741 gatcaaattg aatgatggac aatcggtcat tttcaatttg tctaaactat aagtttcata
    52801 aaaaactgtt aaagggcgaa tattgccata attcagtgag ttcagttgat gaaagaagga
    52861 aaataacatg gaaaatttta aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc
    52921 taatattcta gttgctatca gcaaatttgt cggctatgca atcagtggca gtgccgccat
    52981 gctaaacgaa agcatccaca gtatcgttga ttgcagcaat cagattttgc tgttattcgg
    53041 tgataaacaa gccaataaag gacagagcga gttgcatcaa tttggtgaag gacgagcaaa
    53101 atactttttc agtacaatcg tcgcaatgat gcttttcttc ggcggtgggg cacttggggt
    53161 gatggaagct tttgaaaagt tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt
    53221 tatagcaata ctgttatttg gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa
    53281 agaaatcaaa gaattgaata ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg
    53341 acacagtgaa atactgatta tttttacgga agacttgtgt gctgtaatcg gtttggtctt
    53401 ggctttagca ggaacaatat tgacttatgc aaccgggatt gctgcatttg atgcgatcag
    53461 cggattgctg atcggtttct tgctgatggg agccgccatt ttcctagcaa aagaatttta
    53521 tagtctgatc atcggtgaaa gcgtgacagc aagcgattta tcaaaaatca agttagcttt
    53581 tgaacgtcct gagatcagtc ggctgatcga tataaaaaca gttcatttaa gtccaacgga
    53641 gattttagtt gcagcaaaag cagatatcgt tggtaacaaa gagaatgaag cttactcggt
    53701 catcaatgat attgaaaaaa atatgcgtca gtctcttcct gacaagaaga tgtatatata
    53761 cattgaaaca gataaatatg acccaaatta ttctcgtaaa taaaacgttt attcatgaat
    53821 tgaagcaaaa atgagaaaaa tataatcttg tttttaaata aaacggctac aatttagcga
    53881 atttatgtta caatgagtcc gctgtttatt tgttgtaatt aaaggagata ttttaatgaa
    53941 caaaaagaaa agtgcgccat ggcttcaagc tttgcatgca gtgatgccac tttgtataag
    54001 ctatatacca gtaggattag cttgcggagt attgttgcaa aaagttggat ttgatcctct
    54061 cttatcagga ctgttatcga ttctggtctt ttccggtggc gcgcagtttt tagttgcatc
    54121 tatgcttacg acgcaagcat catttgcaac gacattattg atggttttct ttttagaatt
    54181 acgctataca ttattaggag caagtcttgc cggttttatg aaaaaagaaa agcggacgtt
    54241 tttggctgtc ttttctcaat cactgaatga tgaaaactat gcggtgaact acttaaagtt
    54301 ttcaactgac ccttcctgga ataaaagaaa ggctttatat gtcaattggt tctcaatgac
    54361 tgcatgggcc ggtagcaata tgatcggtaa ttttttcggc tcggtgattc gagtagatgc
    54421 tgaccttgtc cattttgctt tgacggccat gttcattttc atgtttatca tgcagatgaa
    54481 aagcgcacta ctgatcttta caggtttatt ctcaggcgta ttaggggtcg tattcatggt
    54541 attattccaa aatactttcg gattgattgc agcaacagtc attgcttcat ttgccggatt
    54601 tatgctggag aaggcattca aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga
    54661 tgtcaatgag ccgctcttca acttcccgga acaggaggcc aatcaccatg actaaactat
    54721 atttgctact tttagttgct tgtctgttca tcgtggcata tatcccgcgc ttgttcccga
    54781 tgctttattt tactcatcga aaagtgccat cctggttcag tgactggatg aaatatgtac
    54841 cggtggcatt atttgcggcg ttagctttta aagatgtttt cattactcat gaacatctgg
    54901 atattgcttg gaatatcaaa atagctgcaa tgattctagt agcaggcgtg gcttacaaaa
    54961 cacgttcgat ggcgttgtca gttttgacag gacttgcgtc tgtctttttg ctgtctatgc
    55021 tttaaactgt tttattttat taaaaacaga gattgcgaca taagttgttc aaactgatct
    55081 atccgaactg catttgcaac agtcaattct ggatgcaagt ttattcgtag ggataaaaaa
    55141 gattagtgtt ggcttatgat gcaagctctt ttttatttta ggaattagag agggatttgt
    55201 ccttctatga ttttttgcta taatagggaa gatgtttatt ttatgaaaga atagttgagg
    55261 gaatttaaat gaataaaaaa ttgatgcaag gaacattttg gctaaccttt gctaatcttc
    55321 tatgtaaagt tttaggggta gtctatttga ttccctggct aagtatgatg gggaacaatc
    55381 aagatggtat gcttgctacg acattgtata atgttggcta tcttccttat ggattgttcc
    55441 tgatgcttgg tacagtcgga tttccaaatg cgattgcaaa aaaagtagct gtagccacaa
    55501 aagaaggaga taacgctgct tgtcggctga tttttagaag tacgatcaat atcatgtttg
    55561 tcatcggtat cgcctctgct gcactgatgt atttatttgc tcctttacta gccggaatca
    55621 gtccgattgc taacgttaat aatgggatac tagctatcag aagtttatgc ccatctttga
    55681 ttgctatccc aattcttagt gcaatgagag gatatttcca aggaaaaaat gatctgcggc
    55741 cttatggaac ttctttgatt atcgaacagg ctgtgcgagt gatcgttatc ttggctggta
    55801 cttattactt gcgcgtattg actgacggaa ccatattaga agctgtattg atcagtacag
    55861 tcgcttcttt cttcggtggg ttagctgcga ttatccatat gtatatcgtc ggacgcagaa
    55921 aagacttctt cgaacttaaa gattttttga tttccagccg ttatttcgaa agagaaaacc
    55981 gatctgcctc tgtctctatc attcgggaaa cactaccgtt tatttttgtc ggctcagtca
    56041 ttactatcgt tcaaatgatc gaccaagtaa cgatgaaacc actgcttcac tttttccgtc
    56101 cagagatcgc agatcagcaa ctggaattct tattcagccg tgcttctgtc aatccaaata
    56161 aattgacatt gatcctgatc tccatggtag gaacagtagc tataagcagc ttgccaatat
    56221 taagcacgat gaagaaaaaa gatcggatac aaatcgaaaa aacagtagga gatagttttt
    56281 cgattgcgtt attgattctt cttccttcat tgactggaat gtctttatta gctggaccac
    56341 tatacacatt gttttttggc tatgatcctg aaagtgtcgg atatttccaa atggctttgc
    56401 tagcttcttt gtttttctct ttattcacga tcctatctac gatgcttcaa tctttgaatc
    56461 accatcgttt tgcgatccga ttaactgtag aagcaattat cctgaaagtc atcttccaag
    56521 taatcggatt aggattagtt ggcggatacg ggatgagctt atcaaatggc ttagcattcg
    56581 gtattgtctt tgttcgcggc tatcacttta tgtgcaaaga ataccgaata gcacctttag
    56641 caaagatcag caattttttc ttaaaaactt tccgctccac cctgattatg cttgcagtat
    56701 gttttgccgt tttctttttg ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa
    56761 tgatttattg tgtggctgta ggaagtattg gcggcttagt atttttattt tctcaattcg
    56821 gaaaaaatgg gtttcgaatg ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg
    56881 ttaactaatc gttttttgct gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg
    56941 tttttctatt ttccaatttt agtttcatat ctaatcttac ttatgatata gttataaggt
    57001 ctggaaaaat tctttgaaac gaggaaatta aacacatgaa acttgcagta gttacagata
    57061 gttcggcata tctgccggaa cgtatcaaga accatcccga cttattcgtt attccaatcc
    57121 ctttgattat ggatgggaaa atttataacg aaggaatcga tatcgaagcg gatgaatatt
    57181 atggactttt aaaaaacagt aaagattttc ctactacttc acaacctgtc gtaggggaag
    57241 ttttgggttt atatgaggaa ttgaaagaga aagggtatga cacgatttta agtatccatc
    57301 tttcttcagg gatttctggc tttgtcaaca cattatttgc gatgaaagat gacatcagcg
    57361 gagtaacggt cattccttat gactctaaga tcactagtat gccgatggga cacatggtag
    57421 aagctgcctt agacttaaat gatgaaggtc agtcccttga tgaaatactt gcacatatcg
    57481 atcgaatccg agataatacc tatgcttatc tgattgtaga tgatttgaac aatttagtac
    57541 gaggaggacg tttgacaaat ggtgcagcgc tgattggcag cttattaaaa atcaaaccga
    57601 tcctgacatt ttctgaaggg aaaattgttt tattcgaaaa aatccgttca agtaaaaaag
    57661 catttgctcg agctgaagaa attattggta gaagaaataa cgaaatacaa ccgccagtca
    57721 aactttacgt gatacatgca aataatttag aagtagcgat tgcggagaaa gagaaattgc
    57781 agaaaaaata tccaaatgca gaaatcgaaa ttggtcattt cggaccagtc attggtacac
    57841 atttaggtga aaaagccatc ggcattgcca tttcagcgca ataattcctt ttattcaaaa
    57901 aacataccaa aaata
//
LOCUS       DAJSBN010000014        55895 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJSBN010000014 DAJSBN010000000
VERSION     DAJSBN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.014"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            25..1857
                     /gene="tet(M)"
                     /locus_tag="ONT86_000928"
     CDS             25..1857
                     /gene="tet(M)"
                     /locus_tag="ONT86_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2351740.1"
                     /translation="MTELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWENTKVNII
                     DTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFINKID
                     QNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKY
                     TSGKLLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTHRGQS
                     ELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKIKITEMYTSINGELCKI
                     DKAYSGEIVILQNEFLKLDSVLGDTKLLPQRKKIENPHPLLQTTVEPSKPEQREMLLD
                     ALLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIEIKEPTVIYM
                     ERPLKKAEYTIHIEVPPNPFWASIGLSVAQLPLGSGVQYESSVSLGYLNQSFQNAVME
                     GIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLE
                     PYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTF
                     FTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1976..2143
                     /locus_tag="ONT86_000929"
     CDS             1976..2143
                     /locus_tag="ONT86_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2351741.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2203..2556)
                     /locus_tag="ONT86_000930"
     CDS             complement(2203..2556)
                     /locus_tag="ONT86_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2351742.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            3061..3384
                     /locus_tag="ONT86_000931"
     CDS             3061..3384
                     /locus_tag="ONT86_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR2351743.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYLW
                     EFRYF"
     gene            3363..3692
                     /locus_tag="ONT86_000932"
     CDS             3363..3692
                     /locus_tag="ONT86_000932"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351744.1"
                     /translation="MGISLFLELPAIVSYWVVTVMCFVAMGVSTMFVVQIYTLVQTQT
                     PPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVASIASIMISIYSKR
                     IFQKLEI"
     gene            3919..4338
                     /locus_tag="ONT86_000933"
     CDS             3919..4338
                     /locus_tag="ONT86_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2351745.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDRKISELYHVSRSAICSRRTRGLRKLKMLLSEGK"
     gene            4343..4582
                     /locus_tag="ONT86_000934"
     CDS             4343..4582
                     /locus_tag="ONT86_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2351746.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            4840..5685
                     /locus_tag="ONT86_000935"
     CDS             4840..5685
                     /locus_tag="ONT86_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2351747.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            5966..7186
                     /locus_tag="ONT86_000936"
     CDS             5966..7186
                     /locus_tag="ONT86_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCR2351748.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            7183..9903
                     /locus_tag="ONT86_000937"
     CDS             7183..9903
                     /locus_tag="ONT86_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2351749.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            9887..10831
                     /locus_tag="ONT86_000938"
     CDS             9887..10831
                     /locus_tag="ONT86_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2351750.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(10900..11910)
                     /locus_tag="ONT86_000939"
     CDS             complement(10900..11910)
                     /locus_tag="ONT86_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR2351751.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(12090..12434)
                     /locus_tag="ONT86_000940"
     CDS             complement(12090..12434)
                     /locus_tag="ONT86_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR2351752.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(12427..12861)
                     /locus_tag="ONT86_000941"
     CDS             complement(12427..12861)
                     /locus_tag="ONT86_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCR2351753.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12999..13736
                     /locus_tag="ONT86_000942"
     CDS             12999..13736
                     /locus_tag="ONT86_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2351754.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            13729..14940
                     /locus_tag="ONT86_000943"
     CDS             13729..14940
                     /locus_tag="ONT86_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2351755.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            15144..15926
                     /locus_tag="ONT86_000944"
     CDS             15144..15926
                     /locus_tag="ONT86_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCR2351756.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            15996..16658
                     /gene="trmB"
                     /locus_tag="ONT86_000945"
     CDS             15996..16658
                     /gene="trmB"
                     /locus_tag="ONT86_000945"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR2351757.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(16756..18510)
                     /gene="ade"
                     /locus_tag="ONT86_000946"
     CDS             complement(16756..18510)
                     /gene="ade"
                     /locus_tag="ONT86_000946"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR2351758.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            18642..19571
                     /gene="tadA"
                     /locus_tag="ONT86_000947"
     CDS             18642..19571
                     /gene="tadA"
                     /locus_tag="ONT86_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCR2351759.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            19528..20577
                     /locus_tag="ONT86_000948"
     CDS             19528..20577
                     /locus_tag="ONT86_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HCR2351760.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            20574..20852
                     /locus_tag="ONT86_000949"
     CDS             20574..20852
                     /locus_tag="ONT86_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR2351761.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            20827..21297
                     /locus_tag="ONT86_000950"
     CDS             20827..21297
                     /locus_tag="ONT86_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCR2351762.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            21314..21616
                     /locus_tag="ONT86_000951"
     CDS             21314..21616
                     /locus_tag="ONT86_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351763.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            21633..22004
                     /locus_tag="ONT86_000952"
     CDS             21633..22004
                     /locus_tag="ONT86_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCR2351764.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            21976..22335
                     /locus_tag="ONT86_000953"
     CDS             21976..22335
                     /locus_tag="ONT86_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351765.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            22507..23514
                     /locus_tag="ONT86_000954"
     CDS             22507..23514
                     /locus_tag="ONT86_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2351766.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            23539..24723
                     /locus_tag="ONT86_000955"
     CDS             23539..24723
                     /locus_tag="ONT86_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR2351767.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(25160..26743)
                     /locus_tag="ONT86_000956"
     CDS             complement(25160..26743)
                     /locus_tag="ONT86_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2351768.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(27321..27464)
                     /locus_tag="ONT86_000957"
     CDS             complement(27321..27464)
                     /locus_tag="ONT86_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR2351769.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(27686..28165)
                     /locus_tag="ONT86_000958"
     CDS             complement(27686..28165)
                     /locus_tag="ONT86_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2351770.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(28238..28522)
                     /locus_tag="ONT86_000959"
     CDS             complement(28238..28522)
                     /locus_tag="ONT86_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351771.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(28600..28794)
                     /gene="ssrS"
                     /locus_tag="ONT86_000960"
     ncRNA           complement(28600..28794)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ONT86_000960"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(28866..30152)
                     /locus_tag="ONT86_000961"
     CDS             complement(28866..30152)
                     /locus_tag="ONT86_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCR2351772.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            30290..30961
                     /locus_tag="ONT86_000962"
     CDS             30290..30961
                     /locus_tag="ONT86_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCR2351773.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            30927..31415
                     /locus_tag="ONT86_000963"
     CDS             30927..31415
                     /locus_tag="ONT86_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCR2351774.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            31428..32375
                     /gene="prmA"
                     /locus_tag="ONT86_000964"
     CDS             31428..32375
                     /gene="prmA"
                     /locus_tag="ONT86_000964"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR2351775.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            32376..33140
                     /locus_tag="ONT86_000965"
     CDS             32376..33140
                     /locus_tag="ONT86_000965"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR2351776.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            33373..35586
                     /locus_tag="ONT86_000966"
     CDS             33373..35586
                     /locus_tag="ONT86_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR2351777.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            35597..36043
                     /locus_tag="ONT86_000967"
     CDS             35597..36043
                     /locus_tag="ONT86_000967"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR2351778.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            36214..37473
                     /locus_tag="ONT86_000968"
     CDS             36214..37473
                     /locus_tag="ONT86_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCR2351779.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(37562..38509)
                     /locus_tag="ONT86_000969"
     CDS             complement(37562..38509)
                     /locus_tag="ONT86_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2351780.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            38903..40195
                     /locus_tag="ONT86_000970"
     CDS             38903..40195
                     /locus_tag="ONT86_000970"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR2351781.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            40210..41979
                     /gene="aspS"
                     /locus_tag="ONT86_000971"
     CDS             40210..41979
                     /gene="aspS"
                     /locus_tag="ONT86_000971"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR2351782.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            42088..42963
                     /locus_tag="ONT86_000972"
     CDS             42088..42963
                     /locus_tag="ONT86_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2351783.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            43229..45112
                     /locus_tag="ONT86_000973"
     CDS             43229..45112
                     /locus_tag="ONT86_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR2351784.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(45187..45471)
                     /locus_tag="ONT86_000974"
     CDS             complement(45187..45471)
                     /locus_tag="ONT86_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR2351785.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(45461..46195)
                     /locus_tag="ONT86_000975"
     CDS             complement(45461..46195)
                     /locus_tag="ONT86_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCR2351786.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            46324..46947
                     /locus_tag="ONT86_000976"
     CDS             46324..46947
                     /locus_tag="ONT86_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCR2351787.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            47139..48260
                     /locus_tag="ONT86_000977"
     CDS             47139..48260
                     /locus_tag="ONT86_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCR2351788.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            48267..51395
                     /locus_tag="ONT86_000978"
     CDS             48267..51395
                     /locus_tag="ONT86_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCR2351789.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            51414..54608
                     /locus_tag="ONT86_000979"
     CDS             51414..54608
                     /locus_tag="ONT86_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCR2351790.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(54754..55461)
                     /locus_tag="ONT86_000980"
     CDS             complement(54754..55461)
                     /locus_tag="ONT86_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2351791.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        18345 a         9651 c        11565 g        16334 t
ORIGIN      
        1 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
       61 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
      121 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
      181 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
      241 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
      301 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
      361 caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat
      421 cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga
      481 aacgatgacc ttttagagaa atatacgtct gggaaattat tggaagcatt agaactcgaa
      541 caagaggaaa gcataagatt tcataattgt tccctgttcc ctgtttatca cggaagtgca
      601 aaaaacaata tagggattga taaccttata gaagtgatta cgaataaatt ttattcatca
      661 acacatcgag gtcagtctga actttgcgga aaagttttca aaattgagta ttcggaaaaa
      721 agacagcgtc ttgcatatat acgtctttat agtggcgtac tgcatttgcg agattcggtt
      781 agaatatcgg aaaaggaaaa aataaaaatt acagaaatgt atacttcaat aaatggtgaa
      841 ttatgtaaaa tcgataaggc ttattccggg gaaattgtta ttttgcaaaa tgagtttttg
      901 aagttagata gtgttcttgg agatacaaaa ctattgccac agagaaaaaa gattgaaaat
      961 ccgcaccctc tactacaaac aactgttgaa ccgagtaaac ctgaacagag agaaatgttg
     1021 cttgatgccc ttttggaaat ctcagatagt gatccgcttc tacgatatta cgtggattct
     1081 acgacacatg aaattatact ttctttctta gggaaagtac aaatggaagt gattagtgca
     1141 ctgttgcaag aaaagtatca tgtggagata gaaataaaag agcctacagt catttatatg
     1201 gaaagaccgt taaaaaaagc agagtatacc attcacatcg aagttccacc gaatcctttc
     1261 tgggcttcca ttggtctatc tgtagcacag cttccattag ggagcggagt acagtatgag
     1321 agctcggttt ctcttggata cttaaatcaa tcgtttcaaa atgcagttat ggaggggata
     1381 cgctatggct gtgaacaagg attgtatggt tggaatgtga cggactgtaa aatctgtttt
     1441 aagtatggct tatactatag ccctgttagt accccagcag attttcggat gcttgctcct
     1501 attgtattgg aacaagtctt aaaaaaagct ggaacagaat tgttagagcc atatcttagt
     1561 tttaaaattt atgcgccaca ggaatatctt tcacgagcat acaacgatgc tcctaaatat
     1621 tgtgcgaaca tcgtagacac tcaattgaaa aataatgagg tcattcttag tggagaaatc
     1681 cctgctcggt gtattcaaga atatcgtagt gatttaactt tctttacaaa tggacgtagt
     1741 gtttgtttaa cagagttaaa agggtaccat gttactaccg gtgaacctgt ttgccagccc
     1801 cgtcgtccaa atagtcggat agataaagta cgatatatgt tcaataaaat aacttagtgt
     1861 attttatgtt gttatataaa tatggtttct tgttaaataa gatgaaatat tttttaataa
     1921 agatttgaat taaagtgtaa aggaggagat agttattata aactacaagt ggatattgtg
     1981 tcctgtatgt ggaaataaaa cacgattaaa gataagggaa gatactgaat taaaaaaatt
     2041 ccccctctat tgtccgaaat gcagacaaga aaatttaatt gaaataaagc agttcaaagt
     2101 aactgtgatt acagagccag acgcaaagac gcagagccga taaaatgaga ttaatacaat
     2161 ctcattttat cggctctttc cgttatgtat ggattctttt aattagtctt cgatgtttct
     2221 tgcttcgttg ataccgctgg ctaaagattc cattaaggat agttctttgt ctgtaaagct
     2281 atccatgtat ttctctatct gtaatcgtcg ggtgcttttt accaagttat tagcaggtaa
     2341 gaaaaattca tcaacggaaa catgaagtaa cgatacaagg tcataaagaa cttgtatgct
     2401 ggggtgttgc cctttatttt caatattagt taagtaccgt gggtcaattt caatcaatgc
     2461 tcccacttgt tcacgagtta aacctcgttt caatcgagct tctttaatgg ctaaaccaaa
     2521 ggctctaaaa tcatatttat cttctttttt acgcatagta gaccacctct atacatttta
     2581 ttgttcctac tgaattaaaa acaggtatag aaaaacgtgt tatatggttt ataggtttat
     2641 atttaataaa aagcactact aaacgccaat aaaaaaaacc gttatatggt agtgctattt
     2701 acgctgttaa aatattgtat attacttcca aatggcggtt tgttggaggt caacgtcgcc
     2761 atgaagtaca tcatatacaa taaatttcct tacattgggt tcttgtcaaa aaaagtcgtc
     2821 tatctgcaat agataagtac gtccaccaat gtggttttat aaatcatata gatagaataa
     2881 cagaagcatg taaacagaga aataaatctg tttatatgct tttttggcta ttcagaactt
     2941 ttttacaaag tttatttatc agtaatgcaa caaatccccc tttcacattg ggactaagag
     3001 tgaaaggaga taaacgagca aggctcactt cctttcctag acagaaaggg ggtgagaaac
     3061 atgaaaccat cttcttttca gaccacaata gaaaatcagt ttgactatat ctgtaaacgt
     3121 gctatggaag acgagcgaaa gaattatatg ctttatcttt caaggattgc aaagcgtgag
     3181 gtgtcctttt cggatgttgg cgattatctt gttagccagt ttgcgacaac agataactat
     3241 tcaactgact ttcagatttt tacactcaat gggttatcag taggcgttga aaatgatttg
     3301 ttgagtgaag cattacgtga gttgccagac aagaaacgtg aaattctact gctgttttac
     3361 ttatgggaat ttcgctattt ttagaattgc ctgcaatagt ttcctactgg gtagttacag
     3421 tgatgtgctt tgtggctatg ggtgtatcta ctatgtttgt tgttcaaatt tacactcttg
     3481 ttcagacaca aacaccaccg caattagtag gaaagataat ggctacgctg gtagcagttg
     3541 ctatgtgcgg tcaacctatt ggacaaatgg tttacggtat tttattcgat gtatttatac
     3601 aagaaacatg ggttgttatg tttgtagctt ctattgcttc aatcatgatt tcaatatatt
     3661 caaaaagaat ttttcaaaag ttagagatat aatacaaggg gttaaatagt atgttaggct
     3721 aaaaacaata tataaaagaa aatttgaaat ctgctaaaag gatagtgtta taaaagaaag
     3781 agtaatattg cagtatttaa ggctcgcaca aatatatgtt ttgtgtgggc ttttttctta
     3841 ccatttttct gtatttctaa agggtataaa cggtattccg tttatatatt tcagcattag
     3901 cagaaagggg gtgagattat gaggacatct tctttcgagc atatcgtcag attacagttc
     3961 aattctttaa tgctgattgt cattaagaac aaagtgaaaa gtcgaaacag acagcttgca
     4021 agacattcta aacgtgaagt attgttttgt gaaatagccg aaacgaaaca agttgaacgt
     4081 ggaacgattg atacctgttt ttatgattgt gtatcattta aagtcttgca ctttacaatt
     4141 tatgtatctg atgaaacact tggtacagtt ttacatagac tgtcagaaaa acagcgtagt
     4201 gcgattttat tacgttattt tcaaggaatg aacgacagaa aaatttcaga actgtaccat
     4261 gtatcacgtt ctgctatttg cagtagaaga actagaggac taaggaagtt aaaaatgctt
     4321 ttgagcgaag ggaagtaaag aaatgagtaa agattatggc tatccctctt ttgaattgat
     4381 ttgcaaagcg tcaagcggag atgaaatggc agttaaagag attttgaagt tttatgacgg
     4441 ttatatttct aaattatgct tgcgtccttt ttaccactct gaaagtggta aaatcattat
     4501 gcaggttgat gaagaactaa aaggagaaat tcatacagac atgatgaaag caatattgaa
     4561 atttgaaatc ggagtgaagt aatcaactgt ataaaaaaat cgggagatac acagtttcgt
     4621 gctatctccc ttagtagttt tacggtcata tcaaggctca ttcaatcgtg gtaaattcgt
     4681 ggtaagatga gtgtttttct atacttcaaa atgcttgaat gtatagtgtt tatgcggata
     4741 attgaaaatt agatataaaa tttcttacaa ttagtatatg ttttgtgaga aatttagact
     4801 tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt
     4861 ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca
     4921 agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta
     4981 gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat
     5041 ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat
     5101 gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga
     5161 agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt
     5221 gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc
     5281 ccttataata atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt
     5341 atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta
     5401 aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct
     5461 aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc
     5521 gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca
     5581 atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat
     5641 aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt
     5701 atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact
     5761 tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg
     5821 tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt
     5881 tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata
     5941 aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat
     6001 ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa
     6061 gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata acgttgattt
     6121 tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca
     6181 tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg
     6241 aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt
     6301 atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc
     6361 tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc
     6421 ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca
     6481 aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg
     6541 ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc
     6601 aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga
     6661 attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac
     6721 aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca
     6781 gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga
     6841 ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag actatttgtt
     6901 agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa
     6961 tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca
     7021 atactatcag acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga
     7081 agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa
     7141 gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc
     7201 gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag
     7261 ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata
     7321 ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca
     7381 gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc
     7441 tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat
     7501 gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact
     7561 ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg
     7621 ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat
     7681 cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta
     7741 aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt
     7801 ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa
     7861 ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta
     7921 tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt
     7981 cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa
     8041 ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt
     8101 tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa
     8161 ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag
     8221 attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa
     8281 gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt
     8341 tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac
     8401 ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca
     8461 tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca
     8521 atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac
     8581 aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa
     8641 gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa
     8701 aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat
     8761 cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata
     8821 gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa
     8881 gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa
     8941 gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag
     9001 atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg
     9061 aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta
     9121 tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg
     9181 aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa
     9241 gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa
     9301 gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga
     9361 gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa
     9421 ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca
     9481 gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt
     9541 ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac
     9601 ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg
     9661 gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt
     9721 gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct
     9781 aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat
     9841 taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg
     9901 tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag
     9961 aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat
    10021 cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt
    10081 cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca
    10141 tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc
    10201 tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc
    10261 gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt
    10321 tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac
    10381 aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt
    10441 gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc
    10501 gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat
    10561 aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg
    10621 tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat
    10681 aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt
    10741 gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa
    10801 ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa
    10861 aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt
    10921 ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga
    10981 agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac
    11041 attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac
    11101 tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc
    11161 tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta
    11221 ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt
    11281 caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt
    11341 aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga
    11401 gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa
    11461 tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc
    11521 atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata
    11581 ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc
    11641 taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc
    11701 atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga
    11761 ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc
    11821 gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt
    11881 agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta
    11941 tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc
    12001 aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat
    12061 agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca
    12121 gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta
    12181 aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt
    12241 tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc
    12301 agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg
    12361 ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa
    12421 aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc
    12481 ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct
    12541 gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt
    12601 gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat
    12661 cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc
    12721 tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa
    12781 agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt
    12841 gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat acttcagctt
    12901 aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg
    12961 tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg
    13021 tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg
    13081 gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca
    13141 ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga
    13201 aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg
    13261 aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg
    13321 atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg
    13381 actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct
    13441 ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag
    13501 ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga
    13561 tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata
    13621 acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg
    13681 attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt
    13741 tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg
    13801 aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct
    13861 aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt
    13921 tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt
    13981 tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct
    14041 tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg
    14101 gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg
    14161 aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt
    14221 cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat
    14281 tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt
    14341 ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat
    14401 aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga aattacggca
    14461 aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa
    14521 aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt
    14581 tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt
    14641 agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg
    14701 aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag
    14761 caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg
    14821 attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt
    14881 gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga
    14941 aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc
    15001 tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga
    15061 acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa
    15121 agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac
    15181 gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct
    15241 ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa
    15301 aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg
    15361 aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt
    15421 accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca
    15481 tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa
    15541 atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg
    15601 aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt
    15661 atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa
    15721 cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca
    15781 tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc
    15841 aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac
    15901 aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga
    15961 cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag
    16021 cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg
    16081 gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga
    16141 aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat attggagtag
    16201 aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta
    16261 acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag
    16321 tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc
    16381 gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa
    16441 tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat
    16501 atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata
    16561 ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag
    16621 ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa
    16681 aaacattaat atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc
    16741 tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa
    16801 ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga
    16861 taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg
    16921 tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat
    16981 gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat
    17041 cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc
    17101 aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag
    17161 ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac
    17221 cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac
    17281 tttttctacc aaatctttag tcactagact attcgggata atttcgatca catgggcttg
    17341 tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg
    17401 cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga
    17461 ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc
    17521 tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa
    17581 acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc
    17641 aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt
    17701 atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga
    17761 tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg
    17821 ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata
    17881 aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg
    17941 gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt
    18001 catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc
    18061 atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa
    18121 aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc
    18181 agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga
    18241 tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat
    18301 gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc
    18361 gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa
    18421 gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac
    18481 ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa
    18541 gtataacata aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa
    18601 aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat
    18661 cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag
    18721 cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag
    18781 tctcttcggc agatggacag caactgatct caagatttaa gtatttggga cagatggata
    18841 tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag
    18901 ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc
    18961 tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta
    19021 ttttacagaa aaccgtcaga cgtggattat accttttcag cgggacagtc ggatcaggca
    19081 agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag
    19141 atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac
    19201 agacttatga tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg
    19261 gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc
    19321 gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac
    19381 tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt
    19441 tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat
    19501 cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag
    19561 aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa
    19621 ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg
    19681 tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt
    19741 gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa
    19801 cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa
    19861 ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg
    19921 ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg
    19981 gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta
    20041 ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct
    20101 cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat atattccttg
    20161 tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta
    20221 aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc
    20281 tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat
    20341 cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta
    20401 ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat
    20461 cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta
    20521 gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa
    20581 agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga
    20641 tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag
    20701 accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc
    20761 ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac
    20821 gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg
    20881 ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt tccagttctc
    20941 gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa
    21001 cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg
    21061 aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa
    21121 gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct
    21181 agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg
    21241 acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac
    21301 aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt
    21361 agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct
    21421 atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga
    21481 gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga
    21541 aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag
    21601 catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat
    21661 tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa
    21721 aagagtggct aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct
    21781 ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat
    21841 atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta
    21901 tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa
    21961 atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc
    22021 tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca
    22081 ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca
    22141 aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga
    22201 gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta
    22261 gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat
    22321 actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt
    22381 ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg
    22441 tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg
    22501 tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag
    22561 cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat
    22621 ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa
    22681 cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc
    22741 agactgtctc aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat
    22801 caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca
    22861 aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta
    22921 acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt
    22981 gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa
    23041 tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc
    23101 gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc
    23161 gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag
    23221 ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa
    23281 gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat
    23341 gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca
    23401 caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag
    23461 aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc
    23521 aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg
    23581 aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt
    23641 atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa
    23701 atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa
    23761 ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga
    23821 ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa
    23881 ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa
    23941 aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg
    24001 ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga
    24061 aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg
    24121 aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc
    24181 acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc attagctggg
    24241 gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa
    24301 aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta
    24361 ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat
    24421 attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg
    24481 acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga
    24541 catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg
    24601 ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta
    24661 ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc
    24721 taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg
    24781 tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt
    24841 acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt
    24901 ttatttattg attttcagga aaatcccaat attttatttt cattcatctt tataagggac
    24961 attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt
    25021 cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa
    25081 cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt
    25141 tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct
    25201 cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga
    25261 ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc
    25321 tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt
    25381 tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat
    25441 cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca
    25501 atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca
    25561 aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac
    25621 cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat
    25681 gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct
    25741 ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt
    25801 gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt
    25861 ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa
    25921 aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg
    25981 tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact
    26041 caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc
    26101 gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc
    26161 gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg
    26221 ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc
    26281 gtgcttcctg acagtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta
    26341 atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact
    26401 tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga
    26461 ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg
    26521 taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt
    26581 cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatat
    26641 cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat
    26701 tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga
    26761 ccactcaaaa aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag
    26821 aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt
    26881 cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta
    26941 attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt
    27001 tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa
    27061 tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc
    27121 gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt
    27181 gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa
    27241 cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa
    27301 tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc
    27361 tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc
    27421 gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa
    27481 gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca
    27541 ctttttttat tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga
    27601 ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc
    27661 cgatagttgg caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt
    27721 tttctaaatc agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag
    27781 aaacagaacc aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca
    27841 aatctacttg attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt
    27901 ctacgacaga ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca
    27961 atgtttgctt tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg
    28021 tttggaaaga acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag
    28081 ctacattgac cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca
    28141 tgattttttt gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc
    28201 ttttattgta gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt
    28261 tttttgcaaa tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca
    28321 aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat
    28381 atgcagcacc gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa
    28441 aaaagatatc ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga
    28501 taatcattaa aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc
    28561 aatttgccaa gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt
    28621 gtttgtcctg tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc
    28681 gaactaccaa atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca
    28741 ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc
    28801 tatagtagca tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt
    28861 cttgtctagt tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc
    28921 agagcctgtt catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta
    28981 tctggtaaat attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc
    29041 acgccacggt tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca
    29101 tctccagctt tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa
    29161 ttggattttg gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca
    29221 ggcaatccca atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga
    29281 ttacctaaac ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga
    29341 tcacctgcct caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga
    29401 atcgattttt gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta
    29461 agagcttttc tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct
    29521 ttatctgaag gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg
    29581 ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt
    29641 ccaaggccgc gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct
    29701 gttaatggtt tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata
    29761 taaggattct cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc
    29821 agaaaatcct gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta
    29881 ccactcattt ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg
    29941 tttagcatgc gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt
    30001 ccagtaccag gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg
    30061 cgtatgattt ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga
    30121 ggtctcatgc gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt
    30181 cccttaatta tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt
    30241 ttcatagctt atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa
    30301 acaaaagata gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt
    30361 ggggatgtat ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc
    30421 ggaagcatac ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc
    30481 gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca
    30541 tcggattttg aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc
    30601 aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc
    30661 agatatcagc aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta
    30721 tggtcagtct atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa
    30781 aaagatcaat gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc
    30841 gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga
    30901 gaaagattgg ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg
    30961 aaataattga aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc
    31021 atacgatcga gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg
    31081 atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt
    31141 ttaatcctca gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag
    31201 gaaaaaaagg gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat
    31261 tagctcaagg gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat
    31321 tcgagcttca ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg
    31381 aagcagatac ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg
    31441 aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg
    31501 caggggcgag tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc
    31561 aatatggtga gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca
    31621 tggcttattt tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta
    31681 tcacacaatt acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag
    31741 tagaggaaag caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc
    31801 gaatgctgac ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa
    31861 ttatcacact agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga
    31921 cattgacagc actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg
    31981 ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg
    32041 atttagacga agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca
    32101 aagatgtcca tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa
    32161 tcgttgcgaa cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac
    32221 tgaagcaaac aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc
    32281 tggatgccat gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt
    32341 atgcaattat tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat
    32401 catatgaatc aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg
    32461 ttatgaggat ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg
    32521 ttgcagaaat cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag
    32581 aaaatcaaaa ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa
    32641 agctggattg gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc
    32701 cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc
    32761 ttgaaaagat tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag
    32821 agttattgga aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg
    32881 ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg
    32941 aaacaaaacc agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg
    33001 atgaagtcga ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc
    33061 ttcgtacaga aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa
    33121 tgaatccttc tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga
    33181 aaaaatagtc aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc
    33241 gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt
    33301 ttgggacaat tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg
    33361 ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt
    33421 agtcaataca tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact
    33481 gctgcacatg acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa
    33541 gtcgcaggta tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta
    33601 catgatgttg ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat
    33661 gacgttgcta tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac
    33721 gaagagcagc tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga
    33781 gttatcatgg tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga
    33841 gaagataagc aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat
    33901 cgtctaggga tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat
    33961 ccaaaacagt attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa
    34021 tacgtaagtg gtactgttga agatattcga atagcgacag aagagctggg gatttttgca
    34081 gaaatctatg gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa
    34141 aagcagttca acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag
    34201 gattgttatg ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt
    34261 aaggattata ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc
    34321 ggtccggcag gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct
    34381 gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca
    34441 gatggtatga cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc
    34501 tatgatgctt ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac
    34561 gtcttcacac caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt
    34621 gcatacagta tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa
    34681 atggtgcagc ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca
    34741 aattcatttg gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat
    34801 aagatcaaac gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa
    34861 tccgtggtca aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac
    34921 aaactgcaag aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct
    34981 gtagggtatg gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt
    35041 aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa
    35101 aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa
    35161 aacttactaa ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt
    35221 tatatcacta aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct
    35281 gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca
    35341 cgaaaagagt atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat
    35401 gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg
    35461 aataaagata aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta
    35521 aagagtatcg tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat
    35581 ggataggagg acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt
    35641 cgatcagcaa gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga
    35701 aaccgatacg caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt
    35761 tgaagatgaa caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag
    35821 tatctctcaa ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga
    35881 agccgcacga ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag
    35941 tcgcggcatc acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa
    36001 tgatggacct gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac
    36061 tagtaaaaac tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct
    36121 tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt
    36181 atacaagtta ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc
    36241 aaaatcacag atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc
    36301 catgaagcgg acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc
    36361 tacggatcat cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct
    36421 atgccaaaat tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga
    36481 gctgctcatc accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa
    36541 gattatcatc gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa
    36601 tttgaactat ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt
    36661 atcaatttat cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct
    36721 atgatcagcg gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa
    36781 aaatcagaaa aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga
    36841 aaagaaaatg tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta
    36901 cctctggaag tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact
    36961 gtgtgggcgg cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta
    37021 aaaaatgact tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct
    37081 ttcttcccaa tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata
    37141 ccagaaggca gttgggttat tttagattta tatggaacag accatgatga acgaacagtt
    37201 gaagcgccag attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat
    37261 aaagaagaat atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt
    37321 gcaggagaat ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag
    37381 tttgagttct ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat
    37441 ccgaatagcc gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg
    37501 aagatcaaac agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg
    37561 atcaaaatcc acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat
    37621 ctaaagcttg gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag
    37681 acactcgtgg attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc
    37741 ttttagcaat ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt
    37801 ggaaatctgg atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt
    37861 catattttcc tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt
    37921 ttttttcaag ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat
    37981 cttctactac atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt
    38041 ttgacacacc gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg
    38101 attcattttt tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag
    38161 tttgcagacg ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa
    38221 atgttcgccc tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat
    38281 catattcttt ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag
    38341 cataagcggt atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc
    38401 ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc
    38461 caagctgttc tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct
    38521 cctttttgtc tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg
    38581 gaaatagtca gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg
    38641 aatgtcaatg aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg
    38701 aaaatgatct aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg
    38761 tcccgcgtac gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc
    38821 gtgtatgaat ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata
    38881 gctaagaaag ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt
    38941 gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga
    39001 ttatcagtat aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag
    39061 tgtaggggat acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga
    39121 acgccatgtg acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca
    39181 taaattgttc ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt
    39241 tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc
    39301 atttggatca gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt
    39361 caaacagttg gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac
    39421 gcgtcaagcc tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag
    39481 tgaagactct aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa
    39541 acgagatcaa aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc
    39601 tcaaaaacat tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat
    39661 cgacagtaat atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag
    39721 tgacgcacca aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct
    39781 agtggaagaa ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg
    39841 attactcttg actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc
    39901 ttacgtggtc gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat
    39961 tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt
    40021 taagacagca gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc
    40081 tagtcaaacg attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga
    40141 agagatatat gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga
    40201 aaaaaataaa tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa
    40261 caaacagtta cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt
    40321 atcgacctac gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa
    40381 gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa
    40441 gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg
    40501 attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat
    40561 gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca
    40621 aaaatgacta aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta
    40681 gatgataatg actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc
    40741 gcaagagact acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa
    40801 tctcctcaat tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc
    40861 gttcgttgtt tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta
    40921 gatattgaaa caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt
    40981 gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc
    41041 tatgatgaag caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa
    41101 ttgatcgatc taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt
    41161 gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga
    41221 aaagacatgg atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg
    41281 ctgaaagtag aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca
    41341 gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat
    41401 aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta
    41461 ggactaattg atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa
    41521 ttttcagaag aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa
    41581 gaagatatcc cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc
    41641 gtcttgaacg gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa
    41701 gaaaaagtac tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc
    41761 ttgttaacag ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac
    41821 cgttttgcaa tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa
    41881 aacggaaaag cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg
    41941 ttcgaattga atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga
    42001 tttgtcgaat acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag
    42061 ccataacata taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat
    42121 gattacacga ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg
    42181 aatttctttt atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt
    42241 ttgacgactt tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat
    42301 ttactgaata tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt
    42361 ttcactttta tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc
    42421 ctatctaccg atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt
    42481 gggctagcgt tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt
    42541 cgtaaaaaaa caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt
    42601 tttgttgccg gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc
    42661 gtcagtggaa aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc
    42721 gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc
    42781 acgatcatca atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg
    42841 atcgttacac gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa
    42901 gcttttgtaa gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt
    42961 tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc
    43021 agtttttttc taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc
    43081 ttaaagttga aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag
    43141 tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa
    43201 aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg
    43261 ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca
    43321 ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc
    43381 tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat
    43441 ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt
    43501 gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt
    43561 tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta
    43621 tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg
    43681 tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag
    43741 aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat
    43801 cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt
    43861 ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa
    43921 catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact
    43981 gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct
    44041 cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt
    44101 gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta
    44161 tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca
    44221 tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg aactttctta
    44281 tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa
    44341 aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt
    44401 acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga
    44461 tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa
    44521 caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc
    44581 caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat
    44641 gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt
    44701 cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt
    44761 gaaagacact gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga
    44821 tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac
    44881 gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa
    44941 tatgacgctg attgattggt acagctacag taatgaccat agtgactggt tttatgatga
    45001 tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt
    45061 gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca
    45121 aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt
    45181 taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt
    45241 ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga
    45301 aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc
    45361 accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata
    45421 aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct
    45481 cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt
    45541 tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc
    45601 caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct
    45661 gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta
    45721 gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga
    45781 ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat
    45841 tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga
    45901 tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt
    45961 ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa
    46021 aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac
    46081 gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga
    46141 taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt
    46201 ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag
    46261 gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag
    46321 actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga
    46381 aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct
    46441 ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc
    46501 agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga
    46561 tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta
    46621 aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct
    46681 acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca
    46741 gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg
    46801 cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg
    46861 gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat
    46921 ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa
    46981 tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg
    47041 ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt
    47101 gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg
    47161 attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac
    47221 tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag
    47281 acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg
    47341 tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca
    47401 gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac
    47461 agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt
    47521 actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc
    47581 aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag
    47641 tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg
    47701 aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac
    47761 taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc
    47821 cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga
    47881 caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa
    47941 cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt
    48001 acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc
    48061 aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc
    48121 gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg
    48181 aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag
    48241 aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg
    48301 gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga
    48361 tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt
    48421 ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac
    48481 cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg
    48541 aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag
    48601 ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac
    48661 gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc
    48721 aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata
    48781 aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat
    48841 taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc
    48901 ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg
    48961 ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag
    49021 aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg
    49081 aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag
    49141 ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc
    49201 aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt
    49261 cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt
    49321 ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa
    49381 aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag
    49441 acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc
    49501 aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg
    49561 aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc
    49621 aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac
    49681 tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac
    49741 aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt
    49801 tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa
    49861 aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg
    49921 agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc
    49981 ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg
    50041 aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa
    50101 aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg
    50161 ccaatgaaag aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat
    50221 tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag
    50281 aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga
    50341 tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag
    50401 caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg
    50461 aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt
    50521 tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag
    50581 cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag
    50641 ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag
    50701 agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat
    50761 acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt
    50821 atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat
    50881 tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc
    50941 aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat
    51001 ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct
    51061 atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta
    51121 ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg
    51181 tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg
    51241 aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta
    51301 gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta
    51361 gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat
    51421 ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt
    51481 tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat
    51541 tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt
    51601 gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa
    51661 atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg
    51721 agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg
    51781 tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag
    51841 agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat
    51901 atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg
    51961 ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa
    52021 tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac
    52081 aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc
    52141 tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc
    52201 aaggaataca attttctgat caaaacaaac ctttatcatt caaagtggaa aaatttggag
    52261 aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg
    52321 gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt
    52381 ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac
    52441 cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc
    52501 ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag
    52561 caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg
    52621 acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag
    52681 agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg
    52741 aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct
    52801 tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc
    52861 ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg
    52921 atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg
    52981 atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac
    53041 aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc
    53101 atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt
    53161 ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac
    53221 agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt
    53281 tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga
    53341 aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg
    53401 ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg
    53461 ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg
    53521 ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt
    53581 atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca
    53641 gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg
    53701 ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag
    53761 gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga
    53821 ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa
    53881 cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact
    53941 tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga
    54001 tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta
    54061 ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg
    54121 ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct
    54181 tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac
    54241 cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga
    54301 tttccttaaa ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt
    54361 atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg
    54421 gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca
    54481 tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag
    54541 aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg
    54601 aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg
    54661 attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc
    54721 ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc
    54781 taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg
    54841 ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt
    54901 gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc
    54961 cagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt
    55021 gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt
    55081 cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt
    55141 tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa
    55201 gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg
    55261 atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc
    55321 actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac
    55381 gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc
    55441 aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca
    55501 aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc
    55561 ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa
    55621 aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat
    55681 tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc
    55741 tcaatataac tattcaaaca taaaataacg ttatttgatt tatttgttta tagtaaattt
    55801 tatttagtaa tcattgcttt aaaatactac gcttatataa cagagaaaat aagcgttata
    55861 ataaagaaca gtattattta aaatgacaga aaaca
//
LOCUS       DAJSBN010000015        53149 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJSBN010000015 DAJSBN010000000
VERSION     DAJSBN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.015"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            277..1602
                     /locus_tag="ONT86_000981"
     CDS             277..1602
                     /locus_tag="ONT86_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR2351792.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            1595..1945
                     /locus_tag="ONT86_000982"
     CDS             1595..1945
                     /locus_tag="ONT86_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351793.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            1942..2121
                     /locus_tag="ONT86_000983"
     CDS             1942..2121
                     /locus_tag="ONT86_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351794.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            2257..2547
                     /locus_tag="ONT86_000984"
     CDS             2257..2547
                     /locus_tag="ONT86_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR2351795.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            2900..3703
                     /locus_tag="ONT86_000985"
     CDS             2900..3703
                     /locus_tag="ONT86_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2351796.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            3690..4016
                     /locus_tag="ONT86_000986"
     CDS             3690..4016
                     /locus_tag="ONT86_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351797.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            4365..5405
                     /locus_tag="ONT86_000987"
     CDS             4365..5405
                     /locus_tag="ONT86_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2351798.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            6241..6573
                     /locus_tag="ONT86_000988"
     CDS             6241..6573
                     /locus_tag="ONT86_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR2351799.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            7164..7493
                     /locus_tag="ONT86_000989"
     CDS             7164..7493
                     /locus_tag="ONT86_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351800.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            7480..7821
                     /locus_tag="ONT86_000990"
     CDS             7480..7821
                     /locus_tag="ONT86_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCR2351801.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            7935..8174
                     /locus_tag="ONT86_000991"
     CDS             7935..8174
                     /locus_tag="ONT86_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351802.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            8241..8480
                     /locus_tag="ONT86_000992"
     CDS             8241..8480
                     /locus_tag="ONT86_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351803.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            8566..9237
                     /locus_tag="ONT86_000993"
     CDS             8566..9237
                     /locus_tag="ONT86_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR2351804.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            9290..11266
                     /locus_tag="ONT86_000994"
     CDS             9290..11266
                     /locus_tag="ONT86_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR2351805.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            11279..11935
                     /locus_tag="ONT86_000995"
     CDS             11279..11935
                     /locus_tag="ONT86_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2351806.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            12031..12858
                     /locus_tag="ONT86_000996"
     CDS             12031..12858
                     /locus_tag="ONT86_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351807.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            12868..13116
                     /locus_tag="ONT86_000997"
     CDS             12868..13116
                     /locus_tag="ONT86_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351808.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            13159..15258
                     /locus_tag="ONT86_000998"
     CDS             13159..15258
                     /locus_tag="ONT86_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR2351809.1"
                     /translation="MENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQDPVEA
                     RLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(15296..17353)
                     /locus_tag="ONT86_000999"
     CDS             complement(15296..17353)
                     /locus_tag="ONT86_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HCR2351810.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(17467..17679)
                     /locus_tag="ONT86_001000"
     CDS             complement(17467..17679)
                     /locus_tag="ONT86_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351811.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            17866..18333
                     /locus_tag="ONT86_001001"
     CDS             17866..18333
                     /locus_tag="ONT86_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351812.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            18330..18572
                     /locus_tag="ONT86_001002"
     CDS             18330..18572
                     /locus_tag="ONT86_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCR2351813.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            18590..18892
                     /locus_tag="ONT86_001003"
     CDS             18590..18892
                     /locus_tag="ONT86_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351814.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            18963..21017
                     /locus_tag="ONT86_001004"
     CDS             18963..21017
                     /locus_tag="ONT86_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351815.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            21017..22849
                     /locus_tag="ONT86_001005"
     CDS             21017..22849
                     /locus_tag="ONT86_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCR2351816.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            23562..25460
                     /gene="ltrA"
                     /locus_tag="ONT86_001006"
     CDS             23562..25460
                     /gene="ltrA"
                     /locus_tag="ONT86_001006"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR2351817.1"
                     /translation="MTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSNFKNLMK
                     FIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKDYRPRKV
                     KRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNCSTENAI
                     ADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRKMLKAPI
                     VMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHYRKNGAW
                     DKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERLKLPISM
                     EKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIKKIQYAG
                     AKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARGMTKNGI
                     YGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRKLIHQEQ
                     QAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEIECHHIN
                     PYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTYRKKAAN
                     FPISIKMN"
     gene            25577..26377
                     /locus_tag="ONT86_001007"
                     /pseudo
     CDS             25577..26377
                     /locus_tag="ONT86_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            26374..28905
                     /locus_tag="ONT86_001008"
     CDS             26374..28905
                     /locus_tag="ONT86_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351818.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            28955..29287
                     /locus_tag="ONT86_001009"
     CDS             28955..29287
                     /locus_tag="ONT86_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351819.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            29288..29881
                     /locus_tag="ONT86_001010"
     CDS             29288..29881
                     /locus_tag="ONT86_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351820.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            30044..31738
                     /locus_tag="ONT86_001011"
     CDS             30044..31738
                     /locus_tag="ONT86_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR2351821.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            32426..34303
                     /gene="ltrA"
                     /locus_tag="ONT86_001012"
     CDS             32426..34303
                     /gene="ltrA"
                     /locus_tag="ONT86_001012"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR2351822.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            34434..34811
                     /locus_tag="ONT86_001013"
                     /pseudo
     CDS             34434..34811
                     /locus_tag="ONT86_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            34811..36016
                     /locus_tag="ONT86_001014"
     CDS             34811..36016
                     /locus_tag="ONT86_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR2351823.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            36032..36676
                     /locus_tag="ONT86_001015"
     CDS             36032..36676
                     /locus_tag="ONT86_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351824.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            36687..37493
                     /locus_tag="ONT86_001016"
     CDS             36687..37493
                     /locus_tag="ONT86_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351825.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            37515..37745
                     /locus_tag="ONT86_001017"
     CDS             37515..37745
                     /locus_tag="ONT86_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351826.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            38291..38566
                     /locus_tag="ONT86_001018"
     CDS             38291..38566
                     /locus_tag="ONT86_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351827.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            38608..39030
                     /locus_tag="ONT86_001019"
     CDS             38608..39030
                     /locus_tag="ONT86_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351828.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            39049..40641
                     /locus_tag="ONT86_001020"
     CDS             39049..40641
                     /locus_tag="ONT86_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351829.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            40653..40988
                     /locus_tag="ONT86_001021"
     CDS             40653..40988
                     /locus_tag="ONT86_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351830.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            41135..41329
                     /locus_tag="ONT86_001022"
     CDS             41135..41329
                     /locus_tag="ONT86_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351831.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            41361..43733
                     /locus_tag="ONT86_001023"
     CDS             41361..43733
                     /locus_tag="ONT86_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351832.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            43794..45254
                     /locus_tag="ONT86_001024"
     CDS             43794..45254
                     /locus_tag="ONT86_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR2351833.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            45265..47469
                     /locus_tag="ONT86_001025"
     CDS             45265..47469
                     /locus_tag="ONT86_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCR2351834.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            47485..47634
                     /locus_tag="ONT86_001026"
     CDS             47485..47634
                     /locus_tag="ONT86_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351835.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            47710..48303
                     /locus_tag="ONT86_001027"
     CDS             47710..48303
                     /locus_tag="ONT86_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR2351836.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            48316..49215
                     /locus_tag="ONT86_001028"
     CDS             48316..49215
                     /locus_tag="ONT86_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR2351837.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            49218..49703
                     /gene="ssb"
                     /locus_tag="ONT86_001029"
     CDS             49218..49703
                     /gene="ssb"
                     /locus_tag="ONT86_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2351838.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(49844..50047)
                     /locus_tag="ONT86_001030"
     CDS             complement(49844..50047)
                     /locus_tag="ONT86_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCR2351839.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            50122..50382
                     /locus_tag="ONT86_001031"
     CDS             50122..50382
                     /locus_tag="ONT86_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351840.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            50819..51025
                     /locus_tag="ONT86_001032"
     CDS             50819..51025
                     /locus_tag="ONT86_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCR2351841.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            51025..51276
                     /locus_tag="ONT86_001033"
     CDS             51025..51276
                     /locus_tag="ONT86_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351842.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            51291..51728
                     /locus_tag="ONT86_001034"
     CDS             51291..51728
                     /locus_tag="ONT86_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351843.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            51721..52428
                     /locus_tag="ONT86_001035"
     CDS             51721..52428
                     /locus_tag="ONT86_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351844.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            52809..52988
                     /locus_tag="ONT86_001036"
     CDS             52809..52988
                     /locus_tag="ONT86_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351845.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT        19630 a         8355 c        10745 g        14419 t
ORIGIN      
        1 tcaaatgttg taaggatttt attcatttgt taatattaaa gaacggaact ccaaaaaagt
       61 ccgtactcta atttgcagtc tctcactctt ttagagtgag agtttttatt agattttcaa
      121 attaattagt aaagtgatag aataaattga tcatacaaat cataagaaga agcagggtga
      181 tagcatacct atgcttcttt ttttatgtat ttagctattt cttctttaca acatcgaaca
      241 tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc aaaagagcca
      301 gtaaatgatt actttttaat cgatatgaaa agcttttacg caagtgtcga atgtatagaa
      361 agaaatttag atccattaac aacagaactt gttgttatga gtcgatctga taatactggt
      421 tcaggattga tattagcttc ttctcctgaa gcaaaaaagc ggtatggtat tacaaatgtg
      481 agtagaccac gtgacttacc acaaccattt cctaaaacac tacacgttgt tccaccacgt
      541 atgaacctat atatcaagcg aaatatgcag gtaaataata ttttcagaag atatgtggct
      601 gatgaagatc tactgattta ctcgatcgat gaatcgattt taaaagtgac aaaatcactg
      661 aatcttttta cgactgaaga aacacgaagt cagcgaagga agaagctcgc tcaaatgatc
      721 caagaacgta ttaaagaaga gttaggtttg attgctacgg taggcgtcgg cgataatccc
      781 cttttggcta aattagcctt agacaatgaa gcaaagcata atgaaggatt tattgcggaa
      841 tggacgtacg aaaacgttcc tgagaaagta tggaatattc ccgaaatgac ggacttttgg
      901 ggaattggtt ctcgaatgaa aaaaaggctc aaccaaatgg gaattttgag cattagagac
      961 cttgctaact ggaatcctta tacgattaag aatagattag gtgtgattgg attacagctg
     1021 tacttccatg ctaacggaat cgatcgaaca gatatagcaa tcccgccaga accgacaaag
     1081 gaaaaatctt atggtaattc acaagtgtta ccacgagatt atactagaag aaatgaaatc
     1141 gagctggtgg taaaagaaat ggcggaacaa gtagccattc gtatcaggca gcataattgt
     1201 aaaacaggat gtgttcattt aaacattggt acgtcgatct tagaaacgag accaggattt
     1261 tcacaccaga tgaaaatacc tatcacagat aatacaaagg aattgcagaa ttattgtctt
     1321 ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat aacctatagc
     1381 aagcttgtgt acaccgattc gcttcagctg gatttattta gtgatccaca aaagcaaatt
     1441 aacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg ttttacgtcg
     1501 attgtacatg cttcttcaat gttagaaagc gctagaagca ttactagaag cacacttgtt
     1561 ggtggtcatg cgggcggaaa tgggggaatt aaaaatgatt gattacaatg atcgtggacg
     1621 tttaaagtgg gcagggtttt atttatctga tcatactgaa gaaattgatg ccgacaatga
     1681 acaacgaacc agtcaaaatt tagcaaaaga acaaatgacg actgaagaga ttgcggaagt
     1741 attgaacgct gcagtcataa aaaatagatc agtatctatt caaaaagaag aacgaaacgc
     1801 tgaaggatac tatccagaag atattgttgg caagatctta ggtagtgatg agctggggat
     1861 ctacgttgga aaagacaaaa ttcactacga cgaaatacgt catatcgaat tttaccagga
     1921 taataaatgg agtcgattag aatgaacatt atccagcaat atgagctaaa gtatataacc
     1981 tttgatcagc tatcagaaga gatttggggt tatggccaac gtttgataaa tgaggttggt
     2041 gttgagcgct tctctttcta tgtagaagct gcagcaggtt atcataattt taggttttat
     2101 attttcccgt tatatatata aggtatttta cggattctat agagttatct ctatgaatcc
     2161 ttttttgttt ttacaaatag gagtagatac tagaataatg cgtgtgttaa actattattg
     2221 caaacgcaat aataaaagaa agtaggctta cgcaccatgt ctatcaatac atttgtttac
     2281 tcgcacccga tcaatgttta cattatcaag aatttaggaa tcactgtaga acaattttgt
     2341 gagttatatg cctacccaca aggaacggtt gcaagctgga taacccgaca acgtagaata
     2401 aaatcattac ctgcttcatt cgtttatgat ttaagcctgg cctctagttt aaacatgtct
     2461 gatgtttatg aaaagctcct gtctttagaa aaagaatatg attccttcaa gattaaacat
     2521 gataaaaaaa taaaaaaaca tatataatat caatcacaat tgtataatat tacgatatct
     2581 taatatcatg atatcgtaat attgtttttt tctaagaaaa tgcactataa gaagggaatt
     2641 attagtaaca atatagacag tttaataatg tttaaattat tctagttgct gctcgcaaaa
     2701 catatttctt atgattaata aaaaatttta aagtaataaa aagtgattat tttactttcg
     2761 aataacatat gaataatctt tatatccgaa ccgatatata tctttaaaac aaaataatca
     2821 cacaatatta caatataatg catatacgat attgcaatat cacaatatta tgatataatt
     2881 atattataat ataagatgaa tgggggatat tttaatggca agaatattgt cagtagccaa
     2941 tcaaaaaggt ggagtttcta aaactacgac tagcgttaat ttagcagcat gtttgaaatt
     3001 tgtttacggt aaaaatgtac ttttagtgga cattgatcct cagggaaatg ctacagataa
     3061 tgtaggtttc gatatagatg gaacaaacca acctacaatt tatgaagtat taaaagggga
     3121 ggctgacatt acagatgcta ttctggacta taaaggaatt gatgttttgc cagccgacat
     3181 agccctttct tctgcagaga gggaatttac tcaagtagga agtgaacaca ggcttaaaag
     3241 agtacttcaa ccaatcaagg acaactatga ttatattata attgattgtc caccaagttt
     3301 aggaatgctt acagtcaacg catttactgt ttctgatgaa attatcattc cagttgaagc
     3361 tgcgtatttc tcattaaaag gactaataaa gttaagtgaa actattgaaa cggtaaaaga
     3421 atatacgaac gacaagcttg aagtacgagg agtcctattt actaagtata ataagcgtta
     3481 taacatttct aaagaaatga cgactgcagc aaccgaaatt tctagagtca ttggtgcaaa
     3541 aattttcaaa acttatattc gtagaacaat aaccgttgac gaagcgcaag ctgctggatc
     3601 agatctaatc aattttaata aaccctcaac agctgaagat gactataaag catttacaga
     3661 agaatattta aaagaagttg gtgattttga tggcagaaga taatcgcagt caagctgcag
     3721 taagtagact actaggaaat aaaaataaaa agaactacaa accaaaagaa gtagaagaaa
     3781 tcgaattaac caagaaaaat ataagtaaag tcataaaaga tcaaggaagt agtgtaagaa
     3841 cagcagatag cattcgtata aatccaattc taatgtcagc actcaaatat tggacgacta
     3901 ttgctgaatc agacaaaagt aaaccagacg ttatagaaga tgcgctatta aaatccatac
     3961 ctgaagaata cttgattgaa ggatataaac tagcaaagaa acaaaataaa atttaattta
     4021 cattcaatat cacgatatca taatatcgtg atattttttt tataaaaatc agtagtatca
     4081 aagctgataa gtcgcttatc ttcatattgt aatattacga tatcgtgata taatgttatt
     4141 gtaatatcgt gatatagtta ccatgagctt aaaccctgaa atagagcggt ttgctttaat
     4201 ttatttttta aggtaaacta aaaaaagata aaaaaagact actgctttaa caaagtttgg
     4261 cgacttatca gcagtagaaa aatatatagt ggatagatat atttgtttaa ttcattgtaa
     4321 catagttttg aacaaaacgc tatcctacag aaagaggatt ggctatgaat gattttaatt
     4381 attacaaatc aaaagaaatt tatcgtgaaa aatattatca aatgcctaag gtatttttta
     4441 ctaacgaaaa atacatggat ctttcaaatg atgcaaaaat tgcttatatg ctacttaaag
     4501 atcgatttga ttattcagtc aaaaataact gggtagattc tgatgataat atattcttca
     4561 tattcacagt tgaagaatta atgaaattgt tacaatgccg cgaaggtaaa gtatctaaga
     4621 tcaaaaaaga attagaaaaa gcaggtcttc tgaaacagaa gagaggaaga gtaaataaac
     4681 gtgatggtaa gattgaatct atgcctaatt tattgtactt aggcaagcca gaagttacga
     4741 atcaagacgt ctttaaaatc atggaagagg aagacaatac tgattccacc gtaattgcga
     4801 aaatcgcaaa tacggcaaaa ccgcgaaaaa tcaatgattc taccgtaatt gcgaaaatcg
     4861 caaatacggc aaaatccagt catatcaagg actcaaccgt aattgcgaaa atcgcagaca
     4921 atctattcta ttctaattct atagacacta atagacacat aatagacact gaaactgacc
     4981 agttacaaaa tcaagttttg ctagataact tcgtcgacat catgcaagag gacagtataa
     5041 atacatttgt accagaaaat gtattaaact tgataaaaac attttcttca agttacagtg
     5101 aagctcaaca aaccgtccaa actattcata atgcaaagaa aaaagctgaa gaattagagc
     5161 aaactattgt cgtttatgaa gaactggaat cttatggaat tgatgctgac aaaggattat
     5221 atatgacttt actgaaagct tatcaaaaac aaaaaactga aaaagtaaac aatttgcaga
     5281 atctaatttt cgtttatgta aaaaattggt ttgtcgaaaa agctattgcg gcaaagcttg
     5341 caaacgaaca aagagaaaca tttaacgaat taccaacagt tagtacggaa aattggttag
     5401 agtagttgat aaaaagcaat tactataaaa atgatcaatt ttaaattaga acttttttat
     5461 gattttaatt ttattacaaa tattacaaat aaatagttag tattccggag tttttagatt
     5521 aaaagtttat gaaaacaatc acaatcgtag gttttaaatt cataatcgtt agatatttta
     5581 gttatttttc actcaaaaaa atagggaact agtgtttgca tatgtgcttt tttgagttat
     5641 aataattcat gaagagaggt aattcactta tttccccaag ctgaaaagcc tgatattact
     5701 tcttttcagg cagtgctgct aatattcaat tacacttttt tgtatatttt tagttgtttt
     5761 aaatttaatt gtctgctatg cttgtgatga attgattttc gttctgcact tttggacgat
     5821 aaatttggac aattgaaaat acaaaaaaaa gaaaggccct gtgagactcg gaaggctggt
     5881 aaccttcgtc gacgtccaca ggtggcatta agatcactcc cttaatgcgt ttacctttca
     5941 gtgttacggt attacccgta acggatttag tataacagat attcaaaagt gataccactt
     6001 tttttggctg tgttaaggtt aaaaatacgt gatattacga tgaatctatg cctaagcata
     6061 atgttataaa aatgtccgtg tggctctttg ccgtatgacg aacgctgaaa ttagagggaa
     6121 cgcatgtttg gatgcgttcc ctcttttttg tttgtcatga acgaaaatca attctctatt
     6181 tttgaaaaat aacaagaaaa acttgttaaa aatatttaaa gtaagtgggt ggattatttt
     6241 atgtcatata caggaatttt gagtctcgaa gatatttgtc actacggtaa acggtgtacc
     6301 gctacagaaa agatcacgaa gaaactatca actggtcaaa gtaaaaccgt tgtgcagtgt
     6361 aagaaatata taatccagaa agacaaagtc tcagaagaaa tgatttatta cattgggaaa
     6421 caaaaacaaa taattttaaa agatcctatc cctttaaaag agctatatcc aacaatcaaa
     6481 catgtttatg accaaaacgg tgtactgatt ggtcgaagaa aaaatggagt cttgcgttgt
     6541 accgcaaaag ggatggggcg tttaatcagc tgaattgatc attctcataa cttgtatttt
     6601 atcgtttttc gcttgcttga aaatattgtc gagacttcta aaggaactta gaatcactag
     6661 aaaaataagg agtagacttg atctatcaac aattcctttc ggggggtgac ggggtaggtt
     6721 tttacgattg gtaacgtaaa aaattggtca atcgagtaaa tgcccttgtt tggatagtgg
     6781 tgaaatgaat ttgtgtaaat aatatgcaca gagtaaacaa gagggaattt agaagttaga
     6841 caagttagaa tagcgcctag attaatcatg agcagctact aagggatcta ggctttgacg
     6901 caaactcatg gaataaggag taggtcttct cgtaatgggg aggacgcaca agaaattgtg
     6961 tcgtgtgctg cagtacatgt agatacttgc gaagacgttg atctttggat caatcgagca
     7021 tttaccgttt tctactaggg tttctcgttt cggacgccat aaacgagtcg atttatttta
     7081 tatatttttg agtgaacgaa aaacgataaa aatattcgtt atgaagtaaa actaatttag
     7141 tagatagcaa aggtggtaga ggaatgattc aaaaatttgc gaaaatgagt aaaactgcat
     7201 ggattgaaaa gcttgaaaat gacttgagtg tcatgcgatt agctccaaag aaattcaaac
     7261 gagagattgc agaaaaagaa gcaattttgt cggtattgaa tgcggtagac gataaatgtc
     7321 cagtaaaaat tagtttttca gatatagcaa caattatcga aggtatttat tatggtgaag
     7381 ttgatgaaga gatatcttct acaaaagatt tattagattt tttggataaa aacaaagata
     7441 cctttatagt actaactgat tatgaggagg ttacaacaaa tgaaaaatta tgatccgaat
     7501 agccgatggg gaacacatac gattaaggtt tctttccaac gttgggatta caaaggattt
     7561 gtgacgttta gaagaggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat
     7621 gatttatacg atcaaaaatt gactgataat ccaattggat tcggcttgct acctgaagat
     7681 gatcaaggta atgaatggtt taaaatgacg cttatgaatg ataatggaga cgaattatct
     7741 gttgaagata cttggtcata tttgagtgat tatattgtca gcgttgaaat cattgaattt
     7801 gtagcggata aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag
     7861 gagtcacgac gattaataaa aaggtagtat taccttaggc attgagtaag gaatttattt
     7921 aggaggaaaa ttagatggcg cgaccagttt tagaggcgat ggatatggat actttgacaa
     7981 aaggaaggta tgtacagctg ttagctcgtg gttattcacc taaagaagta ttcaaatccc
     8041 tgtctaaagg aacagatatg gaaaaagaaa gattacgaaa agaattcgat tattggagaa
     8101 cccataatgg aatcaaggat ttgaaaccag cacggccagt aattaaacga aagaaaaaga
     8161 taacccaaga ctgaagtgta gagcgtatgt gatcataggc ttttttattt ttgcaaaaaa
     8221 caaggaaggt aggaaaactc atgaattatg aagaaatcga aaacagaaaa aaagtgtcga
     8281 aagaaatgga agaaaagttg ctaaaaacga tgaaacaaaa acaccttaaa agattatctg
     8341 tcatgcaata tatcaatgat atgcagataa caggaaaaga gaaagcttgt ttgttaggtt
     8401 caatgaaaaa ctttgaacag ttgagacgaa cttatgtgaa aaaaagttct aattgtcaac
     8461 tattgttaga agtctcatga cattcattaa tagaattcat ttttaaaact ttttctatgg
     8521 aaatgagaaa aagatttttt gttttgtctg aaagggggta gctggatgag aaaatggtta
     8581 tttggttttt taggagtcgc tttaatcgtc ttttgcagtg tgtttggcta tgttagctac
     8641 caaaaacatg aaggaaaggt gtttaagcaa aatatagaaa aaaagatgcc cgtagatcaa
     8701 atcaatgaac atgcaaagag ttataaggag gatgcaacga acgtcaataa tgatatgtct
     8761 ttggggcaaa tgttgtccat acaaaaagaa gcaatcgaaa tgggtgtcaa taaacaagcc
     8821 tttgcacaaa ttcagatacc tgcacttggt ttagcattgc caatttttaa aggggcaaat
     8881 caatacactt tgagtcttgg agcggctact tatttttatg aagatgccga aatgggcaaa
     8941 ggaaactatg tcctagcagg tcataacatg gaaatgccag gtgttttatt ttctgatatt
     9001 caaaagttgt cattagggga ggtgatggac ttagttagta acgatggcgt ctatcgttac
     9061 aaggtaacgc gtaaatttat cgtacctgag tactttaaat taatcgatgg cgttcctgaa
     9121 gagaactctt tcttgtcttt accaaagaaa ggagagaaac ccttattaac gttgtttact
     9181 tgtgtttata cgtcacaagg aaaagaaaga tacgtggtac agggagagct acaataacaa
     9241 ggttttaatc aacgcgagtc gttgaaatat gaaatgagga gagtaaaaaa tggcaataaa
     9301 tcggtatgta aaaaaagctg gaatgcttgt tggaatgtta ggaatcattg caagctgctt
     9361 tcaggggtat caagtacacg cagaagatgt cacgcaaaaa acaccaccag agaaggtcaa
     9421 tatcacggtt cataaattaa tgtacgatca aggaacgcaa ttgaatgttg atattgacgg
     9481 gatcaaaaac gatggttata cgcacgatca atatcctaca ggtgtaacga aatacaacaa
     9541 ggcagattat ggtgatgttg agtttacgtt aggaaatatt acggatcaag tattaccgag
     9601 agaagattct gatctaacca acgcgaaagt ggacgagatt gtacaagatg tagaagacaa
     9661 gggatctaac agtgagtacg tcaagaatgc aaccaacaaa attacttcag ctgtagatga
     9721 aaatggtgag atcactttta ctgatcaacc agcctatgtc aattccaaag ggaatgtata
     9781 tgtggtttat gaaagtaaat ccgctgctgg attagtcaca caaaaagcaa aaccaatggt
     9841 tgtgattgcg ccaatgacag ataaaacagg atcaggattt ttaaaaaata ttcatttgta
     9901 tcctaagaac attgtttcta agttgagttt tgaattaacg aaatttggtg acgatggcgc
     9961 agcccaaagc aaacaaaccc ttttaaaagg ggcaaaattt gagttataca agggcgaacc
    10021 tggaaaagga acaaaattgg gtgatcttgt atcggatgat caaggaaagc tgactgcgac
    10081 tgatttgact ttaggaaaat actattttgt ggaagttcct tcggaagttg tagtgggatc
    10141 agatcaagaa ccaacagcag atcaatattt attaggcgca gacgctcgca acgatgcgca
    10201 taataaatta acctttgaaa ttaccaatga cggcgtgaca agtgacttaa aagcttccta
    10261 cgtgaactat aaagcaccag tgatcgataa aacagtgaca aatggaacag gacaagaaca
    10321 ttctttccaa attggtgacg cagtgaatta tcaaggaacg attcatatcc ctacagatat
    10381 tgcaggtgga acagatggga ttacagtaaa tggtgtgaag agtgaaactt ctccttacag
    10441 cgtatttaag tggggagata cagcaggaca aggactttct tatgtcgctg ctaaagcaaa
    10501 tatcaaagta acaaataaag atggatctgt ggtattaaaa gaaaatacag attataaaat
    10561 ccaaaattca gaaaatggct ttgtgatcga ttttatcgtg aataatggcc aagtaagtga
    10621 tactgtagca agtttacacg gacaagatct tcaaatgact tataacatgt atgtaaatga
    10681 cagcgcagct gttgccaatc cattaacaaa tagcgtggat ttcgtttata acaacaatcc
    10741 atttaatcag gaagaacacc acgaaaaaac gaaagctgac gttgtgactt atggtgcaaa
    10801 attcttaaaa gtcgatagcg gactatttgg aacaggaatc aaagcaacac cgcttgaagg
    10861 cgcagaattt gctgctaaaa acgcagaagg aaaatattat ggtggattag ttgatacgga
    10921 taaagacggt gtcaaagaag cagtatgggt agacgatgta gcaaatgcag cgatcctaaa
    10981 atcagataaa gaaggtcact ttgaaatcac tggtttaact gaaggtgaat attcactgga
    11041 agaaacaaaa gcaccaactg gttaccaaaa attaacagaa gatattgcat ttaaagtaaa
    11101 caaaaactca tttaaagagg aaaatcgaat cactgttaaa aataatcaaa aagcagctat
    11161 tccattaaca ggtagtaatg gtttccaaac gtatgtattg atctcttgct tgttgctagg
    11221 agcaggggca ctttctgcag ttgtttactt taagaagaaa gcctagaagg atcagctaat
    11281 gattattcgt aagcccccaa aaagaagagt catgcacaaa ctgatgatat ttttttggtt
    11341 gctgtcggca gtagcagtct atttaattcc agctgtggaa acaaatgtag cgaaatttca
    11401 acaacagcaa agaattttag ccattgaaaa tcttgaaaaa acagattctt ctttggatgt
    11461 tgcgaaaata gagttaggcg atcctgttgg tgttcttaca attccaagta ttcctttaaa
    11521 actaccaatt tatgacggaa cttccgacaa aattttagag aatggtgtag ggattacgga
    11581 aggaactggc gatattacag gaggaaacgg taaaaatcct ttaatcgcgg ggcacagtgg
    11641 actttataaa gataatcttt tcgatgattt gccttcagta aaaaaagggc aaaaattctt
    11701 tattaaggta aatgaagaac aacatgccta tcaaatttat cgaattgaag aagttaaagc
    11761 agaagaatta caaaaaaatt atttagatta tctcatgcct gaaaaagacc aagataggat
    11821 cacgttaatg acatgcacgc caaaaggtgt taactcacac cgctttttag tgtatggtga
    11881 acgagtgtca ttcagtaaag aagatttaaa actacagaaa aaagcaaaaa aataggaaat
    11941 caatttcatt ggattggcga ttagcactgc tattcgcttt ttttgcgttc ctattatttt
    12001 tatttataaa aaaataagga ggagatcgga atgaataaga gaaaatggct gtttgtttgt
    12061 gtagcagctc tctttgcaac tgcagcttac tcacagatat cttttgctga tagcagtact
    12121 actccaacta ccagtcagac aacagatagt tcaacaactg caacaagtga cacggacagt
    12181 tcgaagaaca ctgcagaaga aacggcagat tcaagtacga gttctactga atcagcggaa
    12241 tcaacgtcag aaagttcttc agagacttca gaaaaactgc aagaagtaga agatacgcaa
    12301 gtaatcaagc aaaacattca tattcaaaaa attatttctt cgaatcgaat caacaatcaa
    12361 ggaaggcaag tgactcagca agaaggtgtc aatggtgcaa agtttattgt ctacgatatc
    12421 acgaatatct tagaagaaat gacacaagat gataatgaga aaaatcaatc ggtagaaata
    12481 attgagtcaa aattgaaaga tcgagcaaaa aaactttcat acgatcagtt gaaaaaagtg
    12541 actgaaggag aaacccaaac aattgatgat caggaaggcg tgattgattt ttcaattgaa
    12601 gtacctgcaa acaaaaaaca agcatactat atcgtcaatg aatcaagccc tgaaaacatt
    12661 tcaaatagcg aagatattgt tttattgaca cctgtttcag atgaaaaggg tgaatttttg
    12721 acggatgttt ggttatatcc taaatcaaag aaatcagagc cgaaagaaga aattaaaaaa
    12781 gttgtatcaa ctggtgtaaa gaaaaacttt tttgataatt gttgggattt tttcactcat
    12841 ttatttttta acgattagga tgaaatgatg aaaataataa aaatgttagt ttttaatcta
    12901 tcttttttag ccgcaacaat attgtgttgc ggtttagttt tttctcaatt atcaatttac
    12961 ccaagatatc aacgattgtt cgttcatttt ctaaatatcc agcatgcttc catgccacta
    13021 gaagtgatta acaagctctt aatcaatcca atttttatgg ctagtttgtt tcttcttgca
    13081 ggattggtaa cgtgggtatt aggaaaaaaa gaatgagaag cttacaatcg tttaacaaaa
    13141 taatagatgg agggcattat ggaaaataaa attcattgca ctacgggaag agtatttact
    13201 ttagtgttgt ttttgttcct tcttcttttt gcaaggggaa ctgcagtcca ggccttagaa
    13261 aaagttgatc cgagtcaaat caaagaaaat aataatctgg tactttatca tgatagtgat
    13321 tcattgagaa atatcgaagt aactgggatc aatggattta agatactaga tgagtattca
    13381 actaaaaaac aaaaagtact tgcttggcca aaagtcaaag atttgaaaag cacgtcaaaa
    13441 attgttgccc attatcaaaa ggtgggcact tatctaggaa aagaaattga ttgcgacgta
    13501 actttttcta attttgtagc gcacgctggg gttaaccaag acggattagc taaaacaatt
    13561 aatgggaaaa agtattataa ttatttgctc tttgataaaa acatttatcg aggctttttc
    13621 atgcagtcaa tcaacgttat gaatgttaaa ctagatttta agtatgcagg atcaaaaaca
    13681 aaagtagctt tgagtgaacc acagccagaa attaattcaa aaatgagatc gatgagagca
    13741 gctggtattg agttgaatga tacatttcta tctatttaca gtttaaatgg acagtatcag
    13801 caacgtgtgt cttggtataa tgacaataat gaatctgtcg gtgaacgtaa tattgatatg
    13861 agagaatttg ttgggtacga aaaaatggat cgcttacctt attttgtcac aacagataca
    13921 gcatgtgcag aatacaaagc tcctgcgtta tcaacaaaca atttaacttc aaaagtagtg
    13981 ggaggacgtg cagaaaaggc ttatagctcg aatgatcatt tcaccgatgt tgtaggagct
    14041 gatacttatc acagaagtgg tgtaacgtat acgcttcaag gcgcttcccc aacattcatg
    14101 attggcgcaa atacgaatag tatgatgttt agctttgata ctgcattgct ttggacacca
    14161 caaccatcga agcctacaaa agaagtgttt aacaaagcta atactgaaga ggcagcacac
    14221 aatattgaca aaaaagtgat tccacaagga tcagatgttt actatcatat tcaccaaaag
    14281 tttgatgcat taacagtcaa cacaatgaac aaatacaaat catttaaaat cactgatacc
    14341 tttgacagca aaaattttga tatggtatcg gatgggaaaa actatgatgg cgcattgtgg
    14401 tatgttgaag cgaatgggac ttggaaaaaa gctgatacag gaggaaaatt aactgtatca
    14461 ggtaatacag tcacttatac tgcttctgac agctttttag ctaatgctgc aaattacggt
    14521 agggactaca ttttgcagct ccatatgaga gcaaaaaatg atgcaaaagg aattgcacca
    14581 aatgtggcta ctgtaacgtt taacaatgat ccgaacaaag gaacacaaaa aacaaataaa
    14641 gtagaaaact atttctctct taaaccaaat aaaaaagtgg aacaagatgg gaaagatgtt
    14701 gatggtcgaa atgatggtaa aaaaggaacg cctacagctc ctttaaatgc aggatctgaa
    14761 gtacaatatc tagtcacaca aaaatggcat acaaaaggtg tagacattgc gctcgatcat
    14821 tacaagcaat ttagtattca agatccagtg gaagctcgtt tgacgtataa agaaggttct
    14881 gcgcgagtga tagatatgag tacaggaaaa gatattactt cagaaggtat actaacctat
    14941 gatagccatt caagaacgct gaaatgggaa gcttccgctg atttcttaaa taacaatctt
    15001 ttagatggac gagaaattca actgatattt acagctaaaa ctcctttgaa tgaagaattg
    15061 gatatcgaca accaagcaaa agtttcagta gaaagtattg agaaagcaac taatgtagta
    15121 acgattgggg taaaacctaa cttacctcag gtcattgtgc caaaaactgg atcgaatcat
    15181 ttagtgctaa ctgttattgg aagtatatta tttcttactt tagcatcatt gggctatgta
    15241 gttttaaaac tcaaataaat ataaaaacaa ggctgatttt cagccttgtt ttcttttatt
    15301 tgaagattgg attcgcacta ataaatacat tatctacgcc aattctacca gcagagtcaa
    15361 ctacaaaatt atgacctaat ccgagaatac tttggttatt ataaagtaaa tttgcattat
    15421 gggcagggct atttttccat cctaccatgg catcgatagc accatcgtct gataaaaggt
    15481 catcaatata cagcccagtt tgacttatgt tttctccctt agcaccatag cagcctaagc
    15541 tttccaataa atcttggaaa gtaccaccac cagggcgata gtgactgaag ctgacttgta
    15601 attctttagc tcgaatatct gcagcctgtt gaacaatatc attgaattgc attggggcaa
    15661 caccattttg ttggcgataa tcatttaacc aagcaagttc tttttgacgc ataaagttac
    15721 gtaattcgtc taatgaaatc tttttatatt tgaatgtgat tgttttattt tgatcgacgt
    15781 tattaatgga tcgttggccg tttttatcca cggtaacggt aggatcgtca tttctgcaca
    15841 agaaataatt ttgatcagtc atatttttcc aaggagtaga gaagctatcc ccttcgtata
    15901 ctgtttttga ttcactagag agaacagcgt tggtatcagc atttacatga ttaacagtca
    15961 cattaaattt acgagtgtag ttgaaaatat aattttgact ttcagtcaca ttgtttaagg
    16021 ttactgaatt tgttggttct acccgatcgt ctaaaacata tccttgaata gaaggagcag
    16081 ttaaagtgac actttcaccg tgagtgacgt tgattatctg ttctttcaaa atatcaactc
    16141 gcccctcatt tttgtagttg ttattgcaca acaaattgat ttgatgcact tgattataat
    16201 tgaatgtgat tgttgtatta cctgtgattg ttttgctttg agtatcatta ccaatcactt
    16261 tgtagctaaa attatcttta gcaccttcgc tatatttaaa tgcttttgcc ttagcagtgt
    16321 aagtagaacc agaagtcact tttgcttgat ctgtagcaat cacttgacct gtttgattgt
    16381 caatatggtt aatggtaacc atatattctt ttggcgtttc aggtttgtgt tgcaaccctt
    16441 taaatgcagc atcaagacta tttttagcat tgtttacttg tgtttgagaa accttagaat
    16501 cgtctagtac ttttttagca ttggataaag cagtctgaaa attattccaa gattcttctg
    16561 taaaatctcc tttagcagtt gattttactc ggttgtataa agcttgtaat tcagctttat
    16621 tcacagtagg aactggtttt ttctttaatc ctttgtaagc agaatctaat ttatttttgg
    16681 cactatccac ttgcttttga gtcgcttttg agtcatttaa cactttctta gcattgttta
    16741 aagcggtttg gaatgcgttc caggtattgt cggtatagtc accttttttc gttcccttaa
    16801 cgctcgtata aagagcttct agagccgatt tattcacttt gggtttagag gtgtcagcgc
    16861 cataccaacc gataccttcg tatttccagc catagtcttt ggttaaccat tgtacttctt
    16921 tccaacttgc agtgtagtga tgtgagccag attttacacc aggaaggaaa aggcggtaga
    16981 tcggtttatt atttttttcg gcagaacgcc aggcaggccc ttcataagtc cagccgtaat
    17041 ttttagtcaa ccatttcact tcatcccaac tagctgtgta atggtgatca cgaagacctt
    17101 tattgtacaa acgatagaca agatggccac cgctagtcgg tgcttcccag gcggggcctt
    17161 cataatttcc ccattttatc tttactagat ggtcacgttc ttttttgcta gaggtgtaaa
    17221 aatgttcatt attatttgga ttgtaaaggc gatacatgac gttagttgaa gcagcgtcaa
    17281 cagatccagc aagactaagt gaggcagcaa atgttccaat agctaaaaca gtttttggat
    17341 taaataattt cattttttca actcctaaat ttaattaaaa atgtttacat gattattata
    17401 tatcggttaa ccgatatagt caataaaaaa taagaaaagc cttatataat tttggctttt
    17461 cttattttat ttgttagttg aagtattaat atcaacgatt tcttcagcca atttatttaa
    17521 tttcatagca tttatgaatc tcatgttgtc tatactagag cgtttgttgg caaggtcact
    17581 tactgtagtt aagggtacgc cagattctat ggatatagaa cgaactgatt taccactatc
    17641 aaacagcaac cattcgattt tctttttatc agcgtacatt tcaatgctcc ttcttgtttt
    17701 ttctttatta tagctctttt ttgtaatgaa tcacaatatc aagagaaaaa ttttctatag
    17761 aaaggtggat ttttatgagt attcgttctc cgtgtatcca aatgaggttt gttgctactt
    17821 tcgttatttg atatactaaa aataaacaac gaaagaagga ataaaatgaa gaaaaagcaa
    17881 ctatggtttg tctctatgtt tgcaatattt tttattattt taagtggatg ttcaagtaat
    17941 aagaaatcgg aagctaaaga agataatagt ttattgcaac caggaactgt ttggaaagaa
    18001 gaagtaggtg gtcttattca taatttaaaa attatagatg atactacctg ggaatattct
    18061 gaaagtgtat ggcatccaga tcctgtacaa ataacagtta agaggcaaaa agactatgaa
    18121 ggtttagaaa gatacaaaat agtagattct gcaggcatta gggaatttat aaacaaacct
    18181 gattcattat ttattgttgt tccctatgag aaggatggcg taaaaaaaat aatttttctt
    18241 ggatctagca aggatgagaa gcgaacgaaa gagaaattaa ttcatgatgg atcacaatca
    18301 aataaataca aattacaaaa gactagcgag tgattgctag tctttttatt tgtcctgaag
    18361 gaggaatcac agtggcaaaa gctaaaacgc catttgaaaa agccgcgtct aaaatcttag
    18421 aacgccgcgg tgtatctgaa acacagtgga aacaagaagt ccttgaaaaa gcacaatttg
    18481 acctattacg gggaaaagac aaggacttag aaggatatat ctatcaacgt gaacgtgaaa
    18541 agcttgtttt cgatgaaatt attaaacact aaaaggagcg aacaatttta tgtttgaatc
    18601 aattacaatg aattttttaa cacaaccgaa agtagtagct gcggttgata tttttagagc
    18661 attacaaact gccgcaacta gtttgaaaaa taatctgatg atttttgctg ctgttgcggc
    18721 agtggcttcc attgctcttg gaggtatttt cttcttcttt gggcgaagag gagctgaaaa
    18781 cgggaagaca cacgtaggtt atgtattgtt gggggtagga gttattttag ctgccgcttc
    18841 tattgtagtt tcaatgttct caatgtttgg acaaacccca caaggactat aaaaaaacaa
    18901 gtagaaggag taaaaagtac gtggctactt tttactcctt tttttatgga aggagaatat
    18961 tcatgttttt tacgatttca ggcaatgcaa aattggataa agagttaata agaaaagcaa
    19021 cgaagaaagt gggaaagcgt acttcctttt taccgaaaac aattaaatat tctgtcattg
    19081 aaacggaaga acagctacat gtgttaggac aagtgttaga acaagagaat caatcttctg
    19141 ttaaattgac tctcacgtta catgataaag aaggaaaaat agaaaaggct ggcacagaat
    19201 acgtttttgg ctcactggat tatcaaattt cagaaggttt ttatcctaca ttgattgatt
    19261 taatccgcct agatatattt aaagatacgg atgaaaccat gagtgtggaa gagtcttatc
    19321 gaaacgaaaa aattttacaa gttttagaag aagagttaga aagcgaccaa agaaaagtga
    19381 atcaagtgag tgaagtcgaa gcaaacgaag aagtaccatg ggaacgccca atctttccaa
    19441 aggatgaatc aaaagaaaaa gagaaaggaa ttattgacga acaagctgac attttgactc
    19501 ataaatcaat atttgaggaa gagaacgagg taatgggtca attggaggta gatccctttg
    19561 aagaaacaat ggaggatgaa gagacggccg aagtagaagt tgatccagtt gaagctaatg
    19621 aagacccaac atggactgaa ggggattctc aggaatatat tgaaccactt atcagtgaag
    19681 aagtagagaa aaatgaaaca gagatacaaa aggcaacggt atttgattat attttggatc
    19741 aatatccgcc agagcatgaa tggatgaaag aaaaaaataa attattttta aatgaacttt
    19801 attcgaaata tcaattacct caaacctatc aagcattttt agagagtaaa gaagaattaa
    19861 tctcacaggg taaaggcaca cttttagaac agttagagtc tgtccaatca acaaattggc
    19921 atgagcttgc acaagaagat ttattagcta cttttgaaga acgtcaacaa gaaacagatc
    19981 aagaaattaa tgagtatgcg acggaacagt cgtcgaattg ggagaaagca aaagaggaac
    20041 taaataaaga agaacagcga gtgattgaag aagaagtaca aaaaattaaa gagcggtacg
    20101 aaaagaaacg tgacaatgcg tattccttgt gtgtagaaaa gatcaacaat tttactcaga
    20161 cgaatcaaga agcattatcg aaagaaaaag aaaacttatt aaaagaacgt ataaaagaac
    20221 gaaaagaaga attttaccga gggttgcgta ctgataagct aaggattagc aaagaattaa
    20281 atgaacaact gaataaactc tatcaaacta cgactgaagt tctgatggaa aaacacgagc
    20341 agatccaaca agaattacaa gcacaaattc caaaatggaa aaaagagtat gaaattcacc
    20401 aacaagaatt gaaagaacaa gaagaaaaac aacggaaaaa agaaaaagag cgcgaacaat
    20461 tagaactaaa aaaacgtgaa caggatcttg cggaacaaaa aatggcgata gaaaaacaac
    20521 ggctagaaca agaaaaggaa aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg
    20581 atacattgcg acaagcacag ttaaattatc tgacacatcc tcaaccagta gctgctgttc
    20641 aaacgccagt gaacaaccaa gcaaatttaa ctccttcacc ccagcgttcc tttaacggat
    20701 ggatggtggg atgtattgca agtttaagct tgcttctagg tggaggaaca gtatttgctt
    20761 ttaatcatat caatcaatct tcacaggctg attctgtagc cacaacggcg tcaatcaagg
    20821 aagaagccca ggaacaagca agaaaagagt atgaacaaca attagaagaa ttattgaatc
    20881 gtaccaatca gtcaacaaca gagtcaagca tggaaaatag caaagagagt gattctagta
    20941 aggctaaaga aggatcaagc gattcaactg tacaatcaaa cacttccgaa agttcagaat
    21001 caaatgaggt gaaataatgc gtgttagaga aacaacgaag cgaaaagaca aacgattcaa
    21061 tttagaaaag aataggtatc tttctcattt ctatcgctat actccctcag gagaagagct
    21121 accagaaaaa catgacaaac gcatttcgaa aaagattttt tatagtggac ttgtatacct
    21181 ccctttagag tatttagtaa caagagtacc agaagttttc aaccgattgg aaattgttaa
    21241 gggaagctta caaggaataa atctgtttca attacaggaa atgccacagg tctttcattt
    21301 tgattcagct ggtttttgga atccatttac tcctacaact agtttgggta cacgtttagc
    21361 aatttcgaca gtttgtgtgg ttccagctgt acttatggga ttgaaaatgg acatgtggtt
    21421 tcgtcctcta gcagatggac aaaaagggga taatcgatta atgaccttaa aagaaatcca
    21481 acagatttat ccgcaaatac ctgaaacaaa aactactttt ccaggttatg gaggtattcc
    21541 aatcacacat tacaaaaaat attggtacat tcaaacagat actgttaata cgtgtattgt
    21601 tggtacctct cgatcaggga aaggtcagat tgaagtgtta gccaccattg acaatttatc
    21661 tcgtgcagaa aaacaaagtt caatggtcgt taatgatcca aaaacagaat tatatgttgg
    21721 tagtaaagat gaactagaag ctagagggta tgatgtatat gcctttaata tcaccgatcc
    21781 actgcaatca atgagtgata atccattggc actcatcgtg aaatactgga aacgaggaga
    21841 catagatact gcaacacagt taaccaatac tttcacaaat acgatttatt atgatgccaa
    21901 cgcttcagat aacaaatact ttaacgaaaa cgcgcagaaa gctgtcaatg cgcttatttt
    21961 tgcgttgtta gaacaagctg atcaaacagg agattactcc aaattgacac cgaataatct
    22021 cgttgagctt ctttctgaga ttggcggttt taattattat ccagatccag acaatgaatt
    22081 caaacagttg aatgccttag atgaatttat gaatcagttg ccacctggaa acattgcaaa
    22141 aaaacaatat ggggcaacga aaattggatc tgataaagcg aagggaaata ttttatcaac
    22201 ggcaatcacg ggattgaatc catttactct aacgaaaatt gcaaagatga cttctcaatc
    22261 aacgattgat tataaatcgg ttgggtttcc aaaatactta gatttgaaat tacatgaaag
    22321 tcttttaaat caacgagtcg tagtagaatt ttatcataag ggaaagaaat ttcatgaaga
    22381 acgtgtcaaa gtaggctata aaggatttag tgaaatcaat tttgattgtc atttagttgc
    22441 aggtgatcaa gtttgtttaa aatacgatca tcaaggaaaa gaatatgtgg cttggtatga
    22501 attttctcaa agagtattga aagacgaaga gggtaatatc gtttatcgga aaaaacgagg
    22561 agaaacccat ttgccagaat tagaaaaaga agccgttcta acattagatg aggaaaaaag
    22621 taacttatta gtagagtcta ttcggatgca ctataccgat aaaccaatgg ctatttttat
    22681 gcttacacct gattatgatc catctaacca tatcattgtt agtatttttc ttagtcagtt
    22741 atataaagaa ttatctacgc aatgtgttaa gactaaagga gataaatgtc accgcaggat
    22801 tcattttatt ttagatgaat ttgggaatag tgtgacctgt ttagcctaag gataaaagag
    22861 gctagttcca acgaacattg gaataacttt actatcgaga aatcaaatga cggggtaacg
    22921 ccctgaaggg tttccactga tactgcgaca tgcgttagta ggatgtgcat ccaaacgcgt
    22981 atgaagctcg ctgaagtcgg cagaagggta ccgtaaggaa agacacgata cgtatctacc
    23041 acgcaagtgc tctagaggta gggtatgtac ctgatatgtc gggtgtccta aaaagttcaa
    23101 taaggtgaga atgatttgat tatctgacaa acttgcgaat ggaatgtctg aaacgaataa
    23161 ctcatagaaa tgtgaggggg aagggggata cgtcctcttt ccattgactc gaggttgagt
    23221 aaaaatgtgt ttctatgaaa aaccttgcat ggttaaaggc tatggagtag tcaacaggca
    23281 aaagcaagca cctaagttga aatctatgga tagatagtaa ggaacagggg aagcatctag
    23341 cacggaggga gcataccgaa gatgtgtttc taaggaaaaa ccgaaatcgg ttataactta
    23401 ggtgaataga tgtgaaagta gagccacgac cagtgaaaat ttcgtaatga aattggagga
    23461 acaggctcaa gtcgttattt ataatataag aaacctattt aaaatcatgg attctcgtaa
    23521 gactaaaaag gtcacgaacc agaatggagc gtgataaaac catgactaac acaaaaggaa
    23581 ttagcttaaa gaacaaacaa ctcagacacg ccgaatacta cgacatgact gagactttcg
    23641 atgaattgca cgcaatgagt gttgcaaaat caaattttaa aaatctcatg aaattcatta
    23701 ttgatgacaa taatatcttt ctagcgtata gaaatatcaa aaagaataaa ggaagtgcaa
    23761 cggccggata cgatggtgtc actataaaag acattgaaaa attggcatgt ggtgactttt
    23821 taactattat taaaaggcga tttaaggatt acagaccaag aaaagttaaa agagtagaga
    23881 tcccgaaacc aaacggaaaa cttcgacctt taggaattcc ttcaatatgg gacaggatag
    23941 tccagcaatg tattctacaa attttagaac caatatgcga agcaaaattt aataagcata
    24001 gctatggatt cagaccaaat tgttctacag aaaacgccat agcagattgt cttgctagaa
    24061 tcaatcaaag taagatgaat tatgttgtag atattgatat aaaaggattc tttgacgaag
    24121 taaaccatac caaacttatg cgtcaaatat ggacaatggg gattcgagat aaacaactcc
    24181 ttgtaattat tcgtaagatg ttaaaggctc cgattgttat gcgaaatggc aatgttgttt
    24241 ttccaaccaa gggtactcca caaggaggga ttttaagtcc actgcttgcg aatatatgcc
    24301 tgaatgaatt tgattggtgg atagctaatc aatgggagga acgaactgtc aaagagctga
    24361 aacaccacta tcgaaaaaac ggagcatggg ataaaggaaa tatctattcg aaattaagaa
    24421 gccacacaag tttaaaagag atgtatctgg tacgctatgc agatgatttt aaaattttct
    24481 gccgaacagg agaagaagcc caaagggctt tttatgccac acaaaaatgg ctcagagaaa
    24541 gattaaagct cccaatttca atggagaaat ctaaagttac gaacctaaat aaagctgaaa
    24601 gtgaattctt gggtttttca ataagactcc acaaaaaggg aaaaaagaaa gtggctttca
    24661 cgtacgtttg tcgcaaagca gaagaaaaca ttaaaaataa actaaaacaa cagattaaaa
    24721 aaattcaata cgctggagca aaaaaaaata ggcaccgagc aataggtgac tacaacatga
    24781 tggtcatcgg tatccacaat tactatcgga tagcttcaca ttgtagtgtc aatttcactg
    24841 agatttatag aagtgttagg acagtaatag aagcaagact gaaagctaga ggaatgacga
    24901 aaaatggaat ctatggacaa gatcatgaag gataccgtcc gtatctaaag tctaagcaaa
    24961 tgagatatat tgaaggtttt ccgatacttc caattggcta tattaaaaaa agaactccac
    25021 tcaacaagcg gagaaatcta aataaatata ccaaggaagg gagaaagctc attcatcaag
    25081 agcaacaagc tatagaaagc tggaaaatca agtatcttgt aaaaggtttt agtaacaata
    25141 agaaatacac ggtggaattt tctgataaca aaatatctaa gtttatttcc caaaaaggga
    25201 aatgcgctat cacaggaaac gaacttttga tggaagaaat tgaatgccat cacattaacc
    25261 catatcattt gtccaaagac gatagttaca agaaccttgt cattatctct agagaaatcc
    25321 atagattaat ccatgcaact agtgaagaaa ctattctgaa actgcttcgg ttactgaaac
    25381 taaatcacta tcaaattaga aaactaaaca cgtatcgaaa aaaagcagct aattttccaa
    25441 tatcaatcaa aatgaattag ataggaaaca aattatattg taattaacga tggaacgccg
    25501 tgtgcggtga aagtcgcacg cacggtgtgg accgggggaa aagacggaga taatatcaaa
    25561 ctcttaccta tcggaattgc caccaatgga taatatggaa ggaatcatga cggtcacagc
    25621 tggccgaaac atgttgtggg atctgtttat tcaatcttat cagcaacttt atgcgaagta
    25681 tggagaacaa aacgcagcga ttatcaaaga aaattgtcag acacatattt atattatgtc
    25741 gacagatgat aataccatcg aagaattttc taaaaaagtg gggaataaga cggttgaaca
    25801 agagaacagt accatcaatt cattaggtat gaatactcat atcaatcgca atgttgattc
    25861 agatcgtatc ttaactcctg aaagaatttc tactttttta gaaggtgaaa cgattgtact
    25921 cgctccttta aaacgacaag atttgaaagg tcataaaata cggccgtatc ctatttttaa
    25981 tcatggggaa acgaatcttc cttatgctta tcaatatatt gaagatttca atccatcgtg
    26041 ggatatcaat gatatagaca tacattgtac acatgcggac ttggatctta aggctttaca
    26101 aattgattat gattatttcc taccagatga atatgctaga aatctctatc atggactgca
    26161 tgatcaatta gaagaaaaaa acgaagaaaa tatgttttat aaaatcgttc aatctcttga
    26221 gaatgagacg gctaaaaaat tgttaattca attttacgaa gaacaagcag cagatcgact
    26281 tcaagaatat gttgaaaagt tgatcaaaaa tgaagaaatt tctgaaatga ttgggaaacg
    26341 gttgttattg gaatctgaaa aggtggtgaa agaatgatca ttggaggaat tttttctggc
    26401 atagccgact ggtggtcagg cttattcgga gaaggggatg caaaccccaa aaccgtaacg
    26461 aaagtcttac aagattatgg taattatctg cagtatcaag atgtgttcag ttggatttgt
    26521 aatacttttt tatgggggtt gatcaaaggg ttatataatc tcaatacttt actagaaaaa
    26581 accatttatc aaagctttga tttaaaagac ttattgaacg ccgctggggt aaatgaacta
    26641 tttcaaagtt tgattagtaa ggtattggct cttttttgtg tcgtcacatt aatttattta
    26701 ggcttgaaat tttttatgag taaacaccca ccaaaagtca aaaatatctt ggttaattta
    26761 tttatggcca tgatcttgat ttttggtggt agttctttaa tcgatgaagg gttaaatatc
    26821 agtaaaagct tttacggaga cgtcactgca gcaaataaag cagataaaac gggttcacct
    26881 gcttttcaaa ttattcaaaa taatgtgatc gatgtcagta cattactagc aactgacccg
    26941 aataaagtag aatccctgcc aacagacaaa agaaatgctt taacagcgaa aaattttaaa
    27001 acagcgaata tcaatgggat tattactcct aaacaagcta aagaaatggc ggataaagat
    27061 aagagtggtt tatcagattt acaaaaggat cggttaagag cgttacaata ccgactggaa
    27121 ctggatgata atggaaaaga aattcctgtg caaatcagtg atgaaggctt agccaaatat
    27181 gtttatactt cagggtatcg tcgatacatt agtagtccag ggattatttt agctggtgaa
    27241 tcttctttgg cggtggccta tttatttatc ctatttacga tcgttacttg cattattgag
    27301 ctggtattta aaaaattcta tttagtgatt gccgcgtcaa cggactttga gacaggacaa
    27361 cggatgaaaa gtgctatcga agatatttct caatccttct tgttattggc atttacaatt
    27421 ttagaattac gaatctatac cttaatgctt tcaggtattg gtgatttgca tgcggcagga
    27481 aagatcaatg gattcttgta tgttgtcgct ttgattgtac taaccattgc gctattcaaa
    27541 ggctctcaat cagttacgaa aattttcggc gttgatacga gtctgaaaaa cggcagtaat
    27601 tctcttctta gcatgtttgc cttaggaaat attgcgaaaa atgtgggtag cggagctaaa
    27661 aaccttgcaa gtgctggtaa gagtggttta agccatttaa atgatattcg taaaaatgga
    27721 tttggccatc gaaaagatgt tacccctgat aacggggaag aacaatcaga cgataaagct
    27781 acaactgtac ctaatagtaa gaaaacagtt tccgaaatgt tcgggaaagc gaaaaatggt
    27841 gtttctaaag ccgcagaagg tcttggttac gtcaatgaac gtgggttaaa aggaacagct
    27901 gaagatctta cagaaaaagc caaagacacc ttagaagata aattagacgg cacgagagct
    27961 aaagaattgg ctgaagctgt gaagaatcca tccgaagcac tagaaaaaca aaaagaaaaa
    28021 gcttcagaag tcaaagaagg actaaaagat gcgtatggtg aaggacaagt caatgcagca
    28081 gtgaaagcta atacaacaac cgaaaaagaa aatcaagaag gtatagtgat tccttcagtc
    28141 aaagatcacc aagaagcgtt gcactctaat gaaaattcga ttgaagagaa aaattccata
    28201 gaagaaagaa attcagaaga aggaaaaaat ggacagttac caaaaactga tccagaacaa
    28261 tatcgctata aacctaacag tgaccttgaa aatgagaagc gaatttcaga tttatcaaaa
    28321 gtaaatcaag aagacaaagc agaaaatgag ggacagttac caaaaactga tccagaacaa
    28381 tatcggtata gatctgttaa ctccgaaaat gaaaaacgca tttcagattt accaaaacga
    28441 aatcaagaag tacaaacgga tgaggattcc atcgttctgc caaatgataa tggaatcgaa
    28501 gttggaactg aaaaatcaac gaaaattggt ttacctaagg cacaaaattt accaatgaaa
    28561 gatagtcgtt tgaatcaatt caaggataat gatcaaagtt ttgttcatca atcacctgca
    28621 ggtactgttc aaatgaaacc aagtcgtttg cctgaaaatg gaaatctgac aactgtttct
    28681 tacaatttat ctgaaagtgg tgagacaggg aatgcgtccg ttcaaaatgt tgtgacaaga
    28741 gaagctgcac caaaacctat tcaaaacgtt catacaggtt acaagccagc aaatgtggca
    28801 tttactgcac caacaaaaat aaatgtgaca agagaaacta aaaacaggaa caatgcattg
    28861 aaagaacaac tattgaggga tatcaaacag gatatcaaac cataaaaggc ctacaagtta
    28921 ggccttttct ttatatgaaa aggagaaatc aattatggat aaatatataa acatgaaaaa
    28981 ccctccaaga tttagtggag aatggaagtt tttcgctttt ttctttttag atctattaac
    29041 gcttggtggc tgcttttttt gggcgaaaat ggtcaataat cttgtccact taccactctt
    29101 ttttgcactc ttgaattatg gcttatggat tgcgatagcg ttagtgattg tttggcgacc
    29161 ctataataac ccaggtcaac gacaaatttt tgcgattatc gagtctattt tttttgcgga
    29221 caagaaccat tatcatgcgt tagatcctaa ccgaaattat catggtacgt ataaggagga
    29281 tgaataaatg cgagtcaaag gacaaaattc agttgaaagg aaatccattt tgcagtttgg
    29341 aaaaccagaa gagcgctcta tcttggactt atttccgtat aaatcctgta cagaagaggg
    29401 agtcttaatt actagagaag atcattttca acgttattat cgtgtagttt ctacagacgt
    29461 ggaaggatta aatgaacaag agaaactaga acgaatgaat cagttgacca ccgtcatgcg
    29521 tacgtatgtg ccaacaatta agttagttag tttgaccaca gagacggatc taacggaaca
    29581 aatcgtgcaa aagcggcagt tattagataa aaatcgtatg gaacagtcct taggaagaaa
    29641 tctacagcga ttaagaaaat atgaaaagaa attagtagag gaaatccagg aattgaaacg
    29701 agcagaacag gaacgccctg atttaagctt ctttttcgtc attgaaggaa agacaatcaa
    29761 ggatttgcaa accagaaacc gtcagttaat gcgatctagt ggcattttag ggttaaaacc
    29821 actctacaaa aaagaattga tcaagattct ttatcgaatg aataatatga atgatgagta
    29881 ggaatgggga agagagaatg aaatatccgt tgatatggtt agaaaaagat gttccgaaag
    29941 aagagaccac tagaaaattt cctcacactt taagcctggg taagttggga aaaggaaaat
    30001 cttttaacca agtaaagaat caaacaagaa gaggtgactg tacatgttag aagcaattaa
    30061 aacagctttt tcaccacaaa atgatcaaaa ggtacttgat ccattaaagc aactaggaaa
    30121 tcgggaaata gaaattttaa aatccaaagg gtatgattta gactttttaa aacaagtgat
    30181 gccacaaggt ggattaaatt tcgatgaaga gtatgtgacc tatggaaatg gttattcacg
    30241 atgtgtgaca gtttatcact acacaaaaaa tcctacgctt ttttggttgg ccaatctcat
    30301 gaacaatcct catacagtgg caacactaga tgttagcaca gatgaaaagc aaaaagtgat
    30361 tgatcaaatc aatcgtgcct tagatgaatt aaatgatcgg tcagataatg aacgaaaagc
    30421 gactgaccga aataaaagcg cggaagaata cctagaacta gcggaatatg cccgaaaatt
    30481 gacttctaat ggggaaattt ctaaacaagt caaagtgaga gtacatgttt acggaaacac
    30541 gattcaagaa gtagatgaac gagcacagga actgatcgaa aatctcaaag ggatggatta
    30601 tcgtgcagtg acctatctgc acaaaacgaa aagagactgg caatcgatgt tcaccatgtt
    30661 cgatgaacaa gctgaatggt ttggaaatat caaagggcaa tcactaccat cgctaaacat
    30721 tggcggaggt ttcccgttcc accaccaaac gctaaaagat ccaggtggaa cacaatatgg
    30781 ggaaaccatg acaggcggcc cgtttgtttg gaatgccacg tataaaaata gtgtgcggtt
    30841 gtctaccaat accttgcttc taggaatgat gggagctggt aaatcgaccg ttcttaaaat
    30901 gatcgcggaa gcccatttag ctgcaggaga ctttatttgg ggctttgaaa agggaaaaga
    30961 ctttattccc tttttaaagg aatataacgg cattatggtg cgtttagacg gatctgacgg
    31021 gatgatcaat cctttagaaa tatttgccac acgtacgtat gatgaagctt ccagtttata
    31081 tgacgatgga tcagtcaaag atttgaagat caatgaagca gcgtcctatc aaacgcactt
    31141 agacaaagtg gtctatcagg tacagttagt gagtcctcag ctaaaaggaa cgatgaaagc
    31201 agaatttaag acctatttaa atcgttttta tgaagagtat ggaacagtac cacgcggctt
    31261 tacttcctca aattcgcgtt caaatactga tacgcaagtc acaggtaaag atccagaagc
    31321 ttatccgacg ttcaaagaat ttttagactt tttaggacaa ctggaattac caggtgcttc
    31381 ccaagagaaa aagaatcgaa aagaagaaat ggaatccatc gtagaatcct tatgtgagac
    31441 gtatggcatg atttttgatg gtcatagtac gattcgacac cttgaccaac aacagttggt
    31501 cttttttgac attgactcta ttacaggctt agatcgaggg gtacaacaat gtcaaatgta
    31561 catggctatg acgttgattt ggaatcaggc attgattcaa ggaagaagac aacgacgttt
    31621 aattaaagaa aaacaaatgg aagaacaaga cttcaaatgc ttcttggcga tgattgatga
    31681 gtgccataat ggtgcgacct gttctgcagt gaaaagctgt gggttgcaag tagtctaaaa
    31741 actattgtaa taactcttat aacgagaagt caaatgagtg ggtaacgcca caaagggctt
    31801 ccactgatac tccgacatgc aacaagtagt ttatgcagac caacgttgtg tgaagctcgg
    31861 tgaagtcggc tgaaggatac cgtaaggtaa aagaaatagt gaataccacg aaagttctct
    31921 agaggtaaag ggtgtatccg ataagtcggg tgtccaaaaa aacttaatat ggtgagtatg
    31981 tacaaacgta ctgacgaact agcgaatgta ctgtccgaaa ggcatagctc ctagaaatgg
    32041 gagagacagt gagcaaaaaa tcactcattg tgctaacttg tggttgagta aaagtcgttg
    32101 ttatgaaaga ccatgctgcg ttacaggcgt aggagaagtt agggggcaaa agctagcatc
    32161 taagttaagg catgtggata gatataaggg aacaggggaa gccatcaaca tggagagtgc
    32221 atactcgatg aagtggtagt aagaaatatg tgaatgacaa ataacttact ttagtgatgg
    32281 tgaaagtaga gtcacgactg atgaaggttc tgtaatggaa ctggaggaac aggctcgatt
    32341 cgtctgtaaa agtcatgaaa cccgttcaac catgggattc tcgtatgact aaaaaggtca
    32401 cgagccaatt ggagcgtgat aaaccatgac aaaagaaaca agtccaaaag ataggaagtt
    32461 gcggcatgca gaatattatg gcatgacgga aatcttcgat aagatatact ccaaaagttt
    32521 gaagggagat aatttcaaga atctaatgca gattatcatt tctgataaca acattgtcct
    32581 agcatatcgt aatatcaaga gaaatcacgg tagtgtgaca agaggagaag atggagtaac
    32641 gattaaagat attgaaaatc tcaaccgtaa tgaattcgta gaaattgtta ggaagcgatt
    32701 cgcacattat aaccctcgga aagtaaggcg tgtagaaatt cctaaatata atggtaaatt
    32761 aagaccgttg ggtattccat caatatggga tagaatcgcg cagcaatgca tcttacaagt
    32821 attagaacca atatgtgaag caaaattcaa taatcacagc tatggtttcc gcccaaatag
    32881 atcagcagaa cacgcaatag cagattgtat ggcccgaatg aatcaaagtc acatggaata
    32941 tgtggtagat gtagatatca aaggtttctt cgacgaagtc aatcatacta aactcatgag
    33001 acaaatctgg tctatgggta tacgagacaa acagcttctc ttaatcatta ggaaaatgtt
    33061 aagagcaccc gtagtacttc caaatgggaa gttgatatac ccaacaaaag gaacaccaca
    33121 aggcggaata cttagcccat tacttgcgaa catcaatctc aacgaatttg actggtggat
    33181 agcaaatcag tgggaggaaa aagatgccaa agagctaaaa catcatttta ggaaaaatgg
    33241 agcttgggat aaaggtaata tctatcacaa gctcagaaag agtacaactc taaaagaaat
    33301 atacctagtg agatacgcag atgattttaa aattttctgt agaaattata atgaagcgaa
    33361 aaaagttttc tatgcaacta aaatgtggtt acaggagcgt ctaaagttac caatatctga
    33421 agaaaagtca aaaatcacaa atctttcaaa agaagaaagt gaatttttag gctttacgat
    33481 gaaattagtg accaaaggta acaaaaaggt tgttaatacg catataggta caaaagcaca
    33541 aagcatgatt aaagtgaaac taaaaaaaca aatcaaacga attcaaaaaa gtcctaatag
    33601 caaagatacc tttatctata ttggagtcta taacagtatg gttattggga ttcataatta
    33661 cttcaaaatt gcgactcatt gtagtgtagc aatgcgaaaa attgcaagag aactcagacc
    33721 aagttttgaa atacgactac gagaacaagg aatctcaaaa gaaggacaat acggaaagta
    33781 cgaaggatac aagccttacc tacggtcagg acaaatacga tatataagag ggtttccaat
    33841 attaccagta gggtatattc aacacagaca tccgatgtgt aaaaaaagaa atattaataa
    33901 atacaccgaa gaaggtaggg aagccattca taagaagcaa caaaccgtag aagaatggaa
    33961 gatatcgtgg ttgtgtagca atccagtgct tagtaagaga gcaacgattg aatttgcgga
    34021 caatcgaatt tcaaaattca tcgctcaaaa gggaagatgt gcagtaacag gtgaggagct
    34081 tatcttatct gaaatgcatt gtcatcatat tatcccttat catgaaagca aaagtgatag
    34141 ctatgaaaac ctagtgattg ttactgagga ggttcatcgg gtaatacacg ctacacaaag
    34201 tgaaacgatt gaggaacttc taaaatatct aaaattaaat ccaaaacaaa aagaaaaact
    34261 caatgagttg agattaaaag ttggtaacga agaaatatct taataaatat ttgtaggacg
    34321 ggaaaacaga atgtctttta tagacgaggg aacgccgtat gcggtgaaag tcgcacgtac
    34381 ggtgtggact gggggaaaag acggagataa tatcaaactc ttacctatca gtatcgatta
    34441 atccagattt tattgaaacg gtaaattatg tcacaaactt tcaacgagaa atgcgtaaag
    34501 tagaagcaat gactgtttta gctacacaat ccccacaaga aatggtgcca gaaaatatgt
    34561 cttctgataa cttctcgaaa ctgaagaaag tctttgaatt ttcttctacg aagatcttta
    34621 tgcgtatgga tgaatctatt ttacgtcata tagaaggatt agttggtaaa tcaatgacaa
    34681 actcagaatt agaatccatt cctcaacaaa atcaaggaga cgctgtgatc aattttggct
    34741 caaaagaaac cattcgtgtt cattttagtc cgaacaaacg tcaattaaaa ttgtttgatg
    34801 gaggaaaata atggaaaacc aaaatgaaag tttaatcaag cagtatgtta agcgtcgtgc
    34861 aaagcgacgc ttatttttgt ggttgtttgg tacttctgca ggtttgatta ctattttgat
    34921 tactgtgttc gtgacccttt ttctaatact tgcggcgggt tcgattgata attctgattc
    34981 agattcaagt agtggtggcg aagcttttac aggagaatat tcggaaggct taccgattta
    35041 taaagaaatc aaaggaagag gccctttttc tgatgagatt gcgcaatatg ctgtaggtgc
    35101 agcagtgaaa tataaattat taccaagtgt gatcctttcg caatacggtt atgaatcagc
    35161 gtttggtacg tcggcgtccg caagaaatga tctcaactat tttgggatta cttggtttga
    35221 tggttgtctg tttcctaaag gaactgcgcg aggaatcggt ggtattgaag gtggctggta
    35281 catgaagttt cccaactcaa aagcagcctt ctcctattac ggctttatgg tagccacaca
    35341 atccaatttt aatgcatgtg taggaaataa aagccctggc gcaagcttat tgatcttagg
    35401 acgaggtggt tatgccgctg caggaattac tgaagatagt ccgtattata caggttgtat
    35461 gtccattatc acttctaata aattaaccga atatgatgaa ttcgccatca aacattgggg
    35521 tgaaggtggc aataacaacg gcacgatcac tggtgaatgg acgaatccat tccctggcag
    35581 cagtttagat aaaagttcct tcagtggtgg tcaattattt ggaacaaatc caggcggtga
    35641 attccgtccg aatggcttcc atgatggatt agactttggt tcagtcgatc atccaggaag
    35701 tgaaattcac gcggtgcatg gaggaaaagt cgtgtacgtt ggcaatcctg gtatttcagg
    35761 tttaggtgca tgtgtgatcg tgattaatta cgacggtttg aatatggtat atcaagaatt
    35821 tgcgaatagt acaggaaatt ctcgtgtgaa ggttggcgat caagtgaaag ttggtcaagt
    35881 aatcgggata cgagatacag cacacttgca tttaggtttc actcgaatgg attggcgtca
    35941 agcacaagga cacgcattca cagacgatgg tacatggatt gatccattac catctttgaa
    36001 tagcagcaaa aaatagatca ggagaaaaaa tatggaaaat aaaactttaa aaatcgtctt
    36061 aacagctgca attagcgtta gtttaatcgg caatatcgcg ttgttttggc agctgaaaga
    36121 agaacaagag acaaatacct ttaaaattga agaagtggct aaaaacgcgt ctgagaagct
    36181 caaaaaaaat tcctctgagg cacaggcgaa gatcgacaag ttaaatcaaa ccatcaatga
    36241 gaaaaaccaa gaaatcgatc agctgaagca acaagatggc acaaaagcaa aagaggaagt
    36301 aactgaagca caaagaaaga cggcagaaga gttcggtcaa ttggccattg ataaatcatt
    36361 aagtagaaat gaattaacgg aaaaactaaa agatgtcgca acccaagaag tgatcgacaa
    36421 attaagtcct gcagacgatg atcaccaaca tgatgattac ggaagttact cctttacatt
    36481 aggtgacgtg caaacttatg ctcaaacaag cagtgtaaaa gaggaacaaa attttgtcgt
    36541 atttgttgat tatacaatcg gcaatccgca attcaaagat gtcaaaccac aaaaaataaa
    36601 aggtggatta actgtcactg aaaaacaagt agatggtcaa tggaaagtca tagactttag
    36661 ttatttcact cgttaggagg aagaaaatga gtaagaaaaa aggagaaaga caacgtttca
    36721 aaaaacgaaa aagttatctt caatggctgg gaatctttat gattctcggc tttttgattc
    36781 ttgctagtcc gatttatacg aatcagaagg tagatatacc agatgaggta agcacgaacg
    36841 cctttttaaa cacgaaagag ctgacaatgg tttcaaaaga aaaaggagcg aaccagcaat
    36901 tagtttttga atttgcgatc gatgatgaag atcaaagctt gttaaaagaa ctggctaatc
    36961 ttaattatcg cgtagaagta aagacagcta aaggggatta tcaagcaata cgagccaaag
    37021 tgatcaaggt gtctgatgat tattttgcag tgaagctaac gcatgtacca gaagaatata
    37081 tcgccatgcg tttaaccatt attcctgaaa aaattgatcc aaaagtggat atgcaagaac
    37141 cacaagatct gatttattac gtgcacgaag ataaggttaa agatcaagtg aaagatgaag
    37201 attatgaaca acacgcgatc aattacaaag tgaaaggata caagaaagaa caaaaagcag
    37261 ctcaaaaaca aatggaatct ttccaagcaa ccattgattt gaatgaagaa ttagtgaaga
    37321 agctaaaaga tcaattgcct tatcaagtcg cagatgatca ggaaaatact gaaaacaaga
    37381 ttaatagcta ccagcaagaa attgaaacat cgaaaacgca actgaaagat caagaagaga
    37441 tcattcaaaa acttcaagaa aaaattgatc gtatgacaaa agaaaagatt tagaaggaca
    37501 ggtggagtga aaaaatgaag cgaacaaact tggctgacaa agatactttc gctgagcaga
    37561 aagtattgat taaagcctat ctcaaagttg taaaagctaa agaaatatgt cgagaagtga
    37621 atttatctaa cacaacaata tctgagttga agaatggtcg aaagaattta gatactttaa
    37681 aattcagatc atttgaagct ctatatgtag ctgctaaaaa atattttaca gctgataaca
    37741 aataaaaaac aggagtattt tatgtgaaaa aagtatataa aaaatgctta tgaatcagct
    37801 atatcacctt aaccatttgt taatgagatg ggagaaaaca ttaaccacga ttctaacgca
    37861 aacatcaagc acataaaaca tttaacactc ctcctttctg gctgaagatt tcaaccaaac
    37921 aggaagacag cgtatcaatt atagcataca ctttttctta aattcatttt tcatatgaca
    37981 aaatatttcc ttctaatcga caaagttaga aggatttttt attgtctaaa tacacaaata
    38041 accttaattt ttaaagagca taaggaaacg tgatatcact ttctcacgaa aggtgttagg
    38101 taggctgtgc ctaccacgtt tctaagaaag ataaatgata tcaaaatttt ggatttgtgt
    38161 gtagatttgt gggtaggatg aaagcgaatg tgggcagtaa aataagcgtt tgtgtgtagc
    38221 aaaatcaaaa aatgtgtagg gatatgtgta gctacacata tccctacaca taataaattt
    38281 ggaggaaaat atggacttat taattagaaa tattgaagaa aagtatatta ataaaattga
    38341 caaacgttgt gaagaactta cggtcaagac tggcaaaaaa tggagtcgaa atcaatacct
    38401 aaaagttttg atcgaaaatg attttgatca cgctcttctg aattataaaa aagaccaatt
    38461 tgatcggttg ttagaaaaat ttgtggatat tcaaacttat aatacgaaaa ctttagaaga
    38521 atatattgca acaaataatc aattaattgg tttgttaatt gaataataaa aatagtattg
    38581 tgaatttata aaaggaggaa atcaattttg gcaaaagcaa caagaaaaca tgtatatctt
    38641 tacgatatgc atatcgaagt acttgatgaa ataatttcta ctcatgaaga tatatctaat
    38701 tatagtgaag cagtcagaca tttgtgtatg atgtatgatc caaaaatcga agaaaaaata
    38761 aatccaattc aaattaaatt gaatgcaatg ggaaaagaaa tttctatttt aaccgaaatg
    38821 gtttcagagt ttgcggatct tcatttgaaa gatacaggaa tactaattgg tacaaattca
    38881 actgtttatc aagaagcaga agaacgtgtg gaaggaaaaa ttaaatccaa tcaaaccaaa
    38941 aaatattctt ctaagttaaa gaaaccagtg aatgaatctg ttcataccag agtagatcca
    39001 tttgatccaa cgaagattct aaaaagataa aagtagaagg agaacgtgat gaataaatcg
    39061 cctgcagtta ttcttacttg tcaatttacc ttgcccaata aaaaatcttt ttcaacatac
    39121 attgattata tgacaagaga aaaagcactg gaagaaatgg aacgacgtac gcccgaagaa
    39181 gaacaggaac tgcaacgaat caagaatgct ttagaggatt tttatattcc tgaaggagaa
    39241 actttttcgg aaagtaagac ggataaagaa ttcagtgaaa cgtcgaatga agctcaatcg
    39301 ttgatggaac atggaatcaa ttttgacaag ttagaggaac aagattttac caaatatctt
    39361 tcttatatga cccgtcacta tgcgttagaa caaaagaaag aactgacgca aagggaacaa
    39421 aaagaatacc aacggttgaa ccaagcaatg aaaaaatatc aaacagagcc acataaaaaa
    39481 gcttcaggaa atcttcctgg agttttttct atggcttcag acgaagtgaa aggaagtgat
    39541 ctgaaggaga tcaaacaaat cttccaaaga ggacaacaaa aaggctcaat catttatcaa
    39601 gatgttatct ctcacgacaa ttcgtatctt gaaaaaatgg gcttgtacga tcccaaaaca
    39661 gataccttag atgaagaagg tttgaaagca gctggaagaa aaatgatgga gacattattc
    39721 gagaaagaac aattgaatga aacaggttac tggatggcaa cgattcaccg taacactaag
    39781 catatccata ttcattttgc agttgttgaa aaagaaaata cgcgtaaacc gtatactgag
    39841 attgaaaatg gtgtgagtta tatcgtaccg aaggggaaga gaagacaagc aacattagac
    39901 gcaatgaaaa ccaattatgt acatgagtta gaacgtttct cttatgaaaa ggatcgaatg
    39961 atcggtatgg agaaacgaaa tttattgaca cgaaaaagtg atttgcgtaa caccttaaca
    40021 aaagtagtaa aagaaccagc acgttacgat cgacacgcca tgaacttatt aaatgaagta
    40081 tataaaaccc tacctgaaaa acgttcggaa tggaattatg gagatgaaaa tcgaaccaag
    40141 ctttcaccat ttacaagggg aaaacttgat gagttaacca aatatgtttt aacaaaagaa
    40201 cctggatatc aagaatatat acagatttca aaagcattga aagaagaagc caaaagcctt
    40261 tatggggaaa gcaaaagaga aacaaagaat gcagaaaaaa acgccctctt tgatttaaaa
    40321 aagcgcacag gaaatgcgat tttaacagaa ctgaagagac aggatagtca aataaaacca
    40381 ttaatagaaa gtgtcaatca gatgcaatct atttacaatg aacgatcctt tcgaacactc
    40441 aaacagttgc caacaaatga atacaagtat agttatcaag aattcgtaaa gcgtaggaaa
    40501 caattccaac aaagagttgt cacgcagcga agcttgcgtc aattaaatcg aacaattaat
    40561 gcacatgaag aaaaatacca cgcaagtaaa gcatatgaag agcaacagcg aagaatccaa
    40621 atggaacaag aaagacaata ggaggaagag aaatgaaaca atatttagat caatggaaag
    40681 tcatagaagg atcactaaaa gaagaacgaa tcgaacaact tcctgattgt ttagaaaaag
    40741 agcatttgtt tcagataagc gaaatgttga gaaacgaaca gtttgatcca aatcaatttt
    40801 tagttgttga gtacccagct actggtgtgt attgttgtaa tcatgtgaag ggtgaaaaat
    40861 attttattat tcaagaatac gaaggtaagt tagcgcctta ttatactact tgggaaataa
    40921 atgaagaggg aatcaataat ttcccatgtg aaagcattga agagtctatt tctctcacgg
    40981 aatgttaaat aaatagagac gtaaaagagt cctgaacgtg attgtccagg actctttttc
    41041 aatgaattgt tttccgccaa ttcactgagt ggtgcgattc aattttatta tatcaaaaga
    41101 aagtgagatt ccaaagtgaa gggaggaaga agaaatgact cagacaacga ttggtgttgt
    41161 ggttgaaaaa agcagggata atctcgtttt tgtgacagaa attcaaacag gtagagcttt
    41221 tgtagtcaca gacaaagctg caaaagcgta tcaaaatggc aatattttaa cgttaaatct
    41281 gactacgaag atgttcgttg atgcagtaga aaattatcca tttgtttaga caaacgatat
    41341 tttacaagga ggaaacggac atggcagaag aaaaagaacc actagataat agtcgtttgg
    41401 gaaagtccaa aagaaaatta gtacggttgc aaaacgaatt aaataatcaa tttcaaaagc
    41461 tatttgatca ccaaaaattg acaaatgggc aaccaatgaa tgacaaacga aatggtcgtt
    41521 catggtttaa ccagcaagaa aaaatagaaa ataaaataca aagtttgaga gaagagatca
    41581 agcaacaaga aaaacaggta gaaaaacttg aaagacaaga agagctaaag gaaatgggtt
    41641 ataacaagta cggtggcctg gacatgacga tcgaaaatat tccaagaatc aaagaagaaa
    41701 tcgaacgctt tgaaaaaggt gaatcgactt tttcagctgc cacgatccga aaataccaac
    41761 gaaaattgga aacattagaa cagctgaaag aaaaatcaga gaaaggaaaa gaaaatcttc
    41821 ttcctgaagt acaagcgatc attgacagcg gacgagtgac gcaatggaaa aagaatccaa
    41881 ctatctattt tctgaaaggc tatcgaaaag tcgctttaga actcaatgaa aacgggggat
    41941 ttgaggaatc taaaaaatac caagccacgt cggaagaaga aaaggcagct gttcgtgaat
    42001 tacttgaaga agctacacct actgattctg tgaaggagaa agaaaccgat catttctatc
    42061 gagtagaatt caacgaaaat gatccagcaa aagggttgaa aagttatggt ggggagatcg
    42121 taacactaga attgatcgat caactcaaaa cactggataa ccaatacgcc gcagaagcag
    42181 gctattataa attctatttt gaagaagtga agaatggaga agtagtcgat cagatacgaa
    42241 tggatctagg ggatggcgtc aatagtaatc accccatcta tttagagtta gctagattgt
    42301 gcgaacaatc acctgaaaag gaacaagaac aaacagtcaa aaatgaacct atttcaaaag
    42361 tacaaaacga gattaaagag acaaaaacaa aacaaaaggg ttatcaaaaa cgaacggcag
    42421 aagagattaa gcaagaagtc aaatcacttt cccaacaggc gcttgatgcg gtgaaaaagc
    42481 atactcattc acctgaagac gtcaaagaac ttttgaattt tatgtctcgt tttccagaac
    42541 gatcctttag aaatcaatta ttgatcgagg aacaatttcc aggcgcaacg gcttgtttag
    42601 gtcgtgccgc gttgaaaaaa gaaggaatct atatcaaaaa aggagaacaa gggaacaaga
    42661 tctttgtcag aaaaacggtc aaaggatttt atgagaaagg acgtggcttt gttcgagaaa
    42721 cagaggctac accagaagat aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa
    42781 aaccttacta cacgattgaa aaagtgtttg atatcacaca aacacaattg aaacctgaag
    42841 actatccgaa aatcttcccg aaccgcgtct ttgattttca attggatcaa acagggcaga
    42901 cagaacttca agttgggatt caagcggtag ccaaaaatat tggaattcaa attcgagata
    42961 tgacagaaag tgaagtctat caaagagaat taggacaagc acgtggggcg tacgttcgta
    43021 acgaatttac tggcgaagaa gaaatcgtca tgaatactcg aaatactcct acgcagcatt
    43081 tagccacaag tattcacgaa ttagcccatg ctagaatgca caaattttca gaattagata
    43141 ctgcaacaaa agaactacag gcagaaatga cctcctatat tgtgtgcaag cattttggga
    43201 tggacacctc ggagaaagct attccttata ttgcgacatg gacgaagaat agtcagacgt
    43261 tggatgataa ggaagctgcg gaacgtggga aaatcatgaa tgatgtcagt cgtgtcgcaa
    43321 atgaattcat tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga
    43381 tccagccagc agaagtgaag caggagcata aaaatactgt agaaacggca gaacaaaaga
    43441 atattgtgcc cgttggctca ctttggatcg acaagaaaga cgggaaagtc ctggaagaag
    43501 acacgggccg aacgttacat ttgaaggatg cggcgtttat tgaagatccg cagcataatc
    43561 aagtgatttt aaaaggaaaa agttatggaa aagagatcca aacggcgtta ccttcagaag
    43621 aatttgcgct aggaaatcca ttccgatttt cacttgagac agtgtcagaa gtggcaaaac
    43681 gaaaaaatga aatctattat gaacaacaat tgtcacaaaa agaacgcgta taaaacaagc
    43741 aagaaagact atctgaaaaa tggatagtct tttttctttt gggaggtttg aaaatgacag
    43801 agaaaaaaat gagtctcatt gatcgatgca agcaaatgga tattgtggat tttgcacgaa
    43861 acaatggttt agcggtggcc aataaaggac gagactatcg cttagaagac catgattcat
    43921 ttgtttttga tcgaagaaaa caacgttttt attggaacag tcaaaatatt agtggcgaca
    43981 ttattgaatt agctaaactt tttttcatag acaaagagat ccaagaccct aagcaacaat
    44041 ttaaagcagc cactgatttt attctaaaga atgaagacaa aacagaacga gtggaaaatc
    44101 ttcattttga gacagaaaag tacaaagatc accccgttga ttatcaacca ctcacagaaa
    44161 aaggaaggaa ttatctgaag gaagaacgaa aactgcccga ttggttgatt gattatgcag
    44221 aaaaagaagg attgattgcc gaactaaagc caaaacatga acggcaaaat ttcttagttc
    44281 gagacgatcg tttggatcat gcggtcgcct ttttatggaa agatccacag acaaaagaga
    44341 cagtaggggc gagttatcaa ggaacgttca ttgattatga acgttttggt gagagaggca
    44401 catataagca tatagataaa aattcgacag cgaatcatgg ctttaaccta aagattggtg
    44461 atcctaaaca gttaaaattt tttgagagta gtattgattt acttagctac gctgcactga
    44521 atcgtgatca gttaaacgat acgtggttag tctctatgga agggttaaaa caccatgtga
    44581 tcagtcatta ttttggtgag gcagtatctg aattgagaaa gaaacaagcc tttcctcaat
    44641 ccatcgagat ttgtgtggat aatgaccgtg caggacatat tttctacgaa aaagaacaat
    44701 taatgggcgc agttgatcca tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta
    44761 atgattggca agtgccaaga gaatacaaag tcatttatga agaagtcgca aaagaagaaa
    44821 aggtcacacc agaagctatt atggccatac acaagacaga aaacaatctg caattaacga
    44881 accaactggt ttccgctcac aaagtcaatg cgtcttttgg tcagcagctt tctgttaacg
    44941 attcaataga agtcattagt ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag
    45001 attgtgaacg ctcgaatggt acgtatgatt ttgatcgttt ttatcaagga aaaggggata
    45061 ttaattctca gattcttttc tcttataaag cagaacaata ctacaaaggc tataaaaatc
    45121 atgaacatga atttgtacct gaagtgaaaa aggattggaa cgatcaattg aagcatgaga
    45181 tccatcaaca ggaaattaga aagcaaaaac gtgcgatgtt gtttcaacaa ggcagacaac
    45241 aagaaaggga gtgagatcgt cacgatgaaa actgtcattt tagcagaaaa accgtcacaa
    45301 gcgaaggcct atgccgatag cttttctaaa gccacccgaa aagacgggta ttttgaaatc
    45361 caagatcgat tgttttcggg tgaaactgtg atcacgtatg gttttggtca tttagtcgag
    45421 ttagattcgc ccgatatgta cgatgaaaat tggaaacaat ggtcattaga gcatttgcct
    45481 attttcccta cacattatca ctatcatgtt cctaaagata agaaaaagca attcaacgtg
    45541 gtgaaacagc aacttcaatc ggccgacacc attattattg cgacagatag cgatcgtgaa
    45601 ggggaactga tcgcttggac gatcattcaa caagcaggcg ctgatcaagg aaaaaccttt
    45661 aaaagacttt ggatcaactc cttagaaaaa gaagccattt atcagggatt ccagcaatta
    45721 cgagacgcag aagagaccta tcccaaattt gaagaagcac aagcacgcca gatcgctgat
    45781 tggttgatcg gcatgaacgg cagcccactg tatagtttac tcttgcagca aaagggcata
    45841 ccaggaagtt tttccttagg cagagtccaa accccaacgc tttacatgat ttatcagctg
    45901 caggaaaaga ttagaaactt ccaaaaagag ccttattttg aagggaaagc ccaagtaacc
    45961 gctcaaaatg gcatatttga tgcgaagctt gatcccaatg aaacccaagc gacgcaagaa
    46021 gcatttgaag cctatttaaa agaaaaaggt gttcaagtag gaaagcagcc aggcacgatt
    46081 cttgaagtcg aaaccgagaa aaaaagcgcg gcaagtccac gtcttttttc tctatccagc
    46141 cttcagtcta aaatgaatca gctcatgaaa gctagtgcca aagatacttt ggaagccatg
    46201 caagggttgt atgaagggaa atatctaagc tatccacgaa cagacacccc ttatattaca
    46261 gaaggagagc atgcgtatct gttggatcat ttagatgaat acaagcattt tttaaaggct
    46321 gaatcattcc ctactcctat tcatacaccg aatagtcggt atgtgaataa taagaaagtc
    46381 caggaacatt acgcgatcat tcctacaaaa acagttatga cagaagcaac gtatgaaaag
    46441 ctgacacctt tacaacaagc gatttatgat caagttttaa agacgacggt ggccatgttt
    46501 gcggaaaaat atacctatga agaaactacg attttgacac aagtccagca gcttcagcta
    46561 aaggcagtcg gaaaagtacc agtagatcta ggttggaaaa agctatttgg caaagagagc
    46621 gacagaaaag agaaggaaga agaaccgcta cttcctaaag taacaaaagg agaaacggtg
    46681 acggtagatc tacaaatctt tgaaaaagaa accaagccac cacaacctta cacggaaggt
    46741 acactaatca ccgccatgaa gacagctggt aaaaccgtgg atagtgaaga agcacaatcc
    46801 atattgaaag aagtggaggg aattggtaca gaagcgacac gagcaaatat tattgaaacc
    46861 ttgaagcaaa aggaatatat caaggtagag aaaaataaac ttgtggtgac gaataaagga
    46921 attttgcttt gtcaggctgt tgaaaaagaa ccgctgctaa caagtgccga aatgaccgcc
    46981 aaatgggaaa gttacttatt aaagattggt gagcagaaag gaacacaagc tactttctta
    47041 gctaatatcc aaaaatttgt ctctcacttg ttggaagtcg taccgggaca aatccagtca
    47101 acagattttg gatcaacgtt acaagaggtg aaagcagcca gtgaaaaaca ggaagcaacg
    47161 cgtcatttag gtgtttgtcc aaaatgtcgg gaacaagaag tcttgcttta tcaaaatgtg
    47221 gcggcatgta cgtctgaagc ctgtgatttt aaattatgga caacgattgc caaaaagaaa
    47281 ctcacagcca cacaactgaa agagatcatt caaaacggcc gaacaagcca atcagtcaga
    47341 ggactaaaag gtcagaaagg ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca
    47401 acaggctttg aattttctga aaagaaaaaa acgaactata agaaaagaac acgccgcact
    47461 acaaaataat gaaggggtta tcctatgcga aatacaacac acaaacaaca agaaatcgaa
    47521 aatcaaaaag caaaaattcg tttagaacaa gagaaattac gtgcaaataa aagaagactg
    47581 caacaattaa agaaccagca gcgcattcaa aaagaaatcg aattagatcg atagacgtta
    47641 atcttgttac aaataaacta ctatgatata tttgatgtag tgtatttgta acgaagggag
    47701 gggaagtaaa tgcaagataa actgaaagaa ttgttggcta ttcgcgatgg aaatttatct
    47761 atgaaggaag ccaggaaatt tggaatcgca gcaaccactg tacaaagatt ggtgaatcaa
    47821 ggaaaattga tcaaagttga tcgtggttat tatgtagagg atggtcatgg aatagatgat
    47881 ttattttggc ttcagcaacg attttccagg ggaatcttct ctcatgaaac gactttagat
    47941 atttaccagc tttctacgaa tatgccgaaa ttcattcact taacttttcc ccatggctat
    48001 aacgttgatc gttcaattac aaaagagcag cagttacaat ttcatttcgt gaagaaagaa
    48061 gtttatgaac taggaaaagt agagggaact tcctttcaag gaaatcctat tactatgtat
    48121 gataaagagc gtacactttg tgatatttgg aatccgagat atgaatttga atatgaaatg
    48181 aaattagaag cattaaaaga gtacatggaa gacccattga gagatccaag taaattaaga
    48241 gattacatga caaaactaca tgtagaaaaa gaaatgaaat accacatgca aagcttatat
    48301 tgatggagga aaaagatgac accgacacaa tttgcagcaa aaacaagaaa tttagcaaag
    48361 agtacaggat taaatgcaca gttagtgcaa cggcatttta tgatggatcg tctaattgaa
    48421 caaatttctg aatcaaagta caaaaatgac ttcattttga aaggcggctt tttattagga
    48481 tcaaaatatg gaatcgatag aaggacaaca attgatatcg atacgacatt tagaaatagc
    48541 aaattaactg aagaaaaatt gaagagcatt tttaatgatt taactagaaa acccacaaaa
    48601 gaaggcattg ttttctctat tcaaggaatg aaggaaacaa gagaagccga tttttatcct
    48661 ggatttcaaa ttaaattggt ggctaattta gagaaagtac gtgtaccgtt taaaatggac
    48721 gtgacaacag gagattctat ttatcctgaa gtagatacac actatcacca attaatgttt
    48781 gaagataaaa aagtggagat tccagcttat ccaacagaac aaattattgc agaaaagcta
    48841 agtgctacgt tcagctttgg cacagataat tcgagagcaa aagattacta cgacttattt
    48901 accattccga aactagaaaa tatcgatacg aaagagttat acaaaagtgt tcgaaataca
    48961 tttacaaaac gtggggatac aaaaccgttg tccttttatt atgaaagaga aatggataag
    49021 atcagaggaa atgactattt ggaaaagcag tgggaaaaat atcgctcagt taacacgttt
    49081 gcgaattcca tttcatttac agatacagtc aacgaaattg atcacctaat gaaaagtgtg
    49141 atcaaaattg aaaatcaaga aagaaaacgg cagtttaaaa gatcacaaca aatgaatcaa
    49201 gaaatggaga gataaagatg atcaataatg taactttagt aggacgctta accaaagatc
    49261 cagatttacg ttataccgcg agtggtactg cagtagcgac ttttacccta gctgtcaacc
    49321 gaaatttcac gaatcaaaac ggcaatcgag aagctgattt tattaactgt gtgatttggc
    49381 gcaaaccagc agaaacgatg gcgacattag ctaaaaaggg cagtttgatc ggcgttgttg
    49441 gacggattca aacccgaacc tacgataacc aacaaggaca acgtgtgtat gtgacagaag
    49501 ttgtggccga taatttccaa ctattagaaa gtaaagcagc gacagaaagc cgtgcgcacg
    49561 ctgatcaatc gtccagcagt ccaagcccta ccacctttga acaacgggat gtagctacgc
    49621 ctaacaacaa tggattgaac gcttcacaaa atccatttgg cggacaatca atcgatatta
    49681 gtgatgatga tttaccattc tgattgtaaa agatcaataa agttgttata atggatacga
    49741 aaagaggagt tgcgctaaca actcccctaa cagcaccgtt taagacggtg accagcttaa
    49801 tattattttt gaaaaataat cgtcgaccgg ctaaagctga cggttatttt ttttgattgt
    49861 tgtttacaac tgctaagatt ccaaaaatga ttgttgcgat caaagaaccg aaactaatca
    49921 ttaatcctag cgccgtttca acaggcaaaa ggcttctcct ttctgtaaga ttttggaagt
    49981 acgcacatac gcaccacctc actttcaatc ggatagccac cgtcacaaac tttactgtct
    50041 tgatcattat agcatatcta aaatcgaata aaaattctca attttagctg tttttgctat
    50101 aattttagcg aggtgatcgt gatgaacaaa tatgttggtt tgcttgataa aatccgagta
    50161 ataaaaacac aaccattatt ggttcgcttt actttacaaa caattcatga atcgattaat
    50221 tgtgtagttg cagatattga aattatcgat aaattgttaa ttatggatga tggaaaatac
    50281 aatattgccg tcacaggtca tttcaataaa cggaatcaat tagtcattga atcgatgcaa
    50341 attagaaacc ccgatcattt tacgcgatcg atgggcatat aaaagaagag ctgcagtcct
    50401 aaatgaggat tgcagctctt tttgttttac atagaaacag gaaaagacct ttttaggaag
    50461 acacaagtgc cttctcaatt gaaaaacctt tctctcactc caaccgccac cgccgttcga
    50521 gaagaaagga agtgaacgtt gcacgttcac taagatatgg tgtcttgcaa agcaagacaa
    50581 aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgctatctc tttattgatt
    50641 tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca agggtaaatg
    50701 gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc aacaaatcaa
    50761 taaagaaaga actaacgaaa ggaaataaaa cctttcctaa gaggaggcag aatcaatcat
    50821 gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa actatcatgc
    50881 accacatact gtcgaatgcg attgtggaga aagagcgaaa tgtatcaaaa gctatcgttt
    50941 gtatcaatgt ccaacatgcg ggaaaaaata taccttgtct tatggcgatt atgttttgat
    51001 tgatgagaaa gtaaagaaac ggtgatgaaa acacaacaca aattattgac tgtaaaagaa
    51061 gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga agctttactt
    51121 attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac tatgctattt
    51181 gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat ggaaacttat
    51241 gaaatcgaat attggttaac caaattagag aactaaaaag gagaaaaaat atggctcaaa
    51301 attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa atggaggata
    51361 tgacgttcct ataccatgaa acacgtgtac ccaaaaaaca ttacaaggaa aaattgtcag
    51421 tcgcagtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg acgtactatc
    51481 gaacactgga agaactgaaa aaacaaaatg gaaaatggtt tttccaagcc ttactatgct
    51541 tggaagtagg tgtcaaacca agcactatca aaccttccga atatcaagcc ttagaactga
    51601 cttatgcaaa attcgcacag attaagaaag ctaaagcaat ggctagcgac tggctagata
    51661 cttttgaaaa tatcgaaaaa aatggtgcat tttatacgat tgaatcggag gagaaagata
    51721 atgaataagg tggtttatca gctaacaaca gaagatgttt cgcaattttt aacaaacaaa
    51781 gaacacttga atgaagtaat cagacggatt caaaaagact ttagttgtat ggaagaaatg
    51841 aagtacttta ttcaaagagt ggaagaacag ttgtatatgg aaagtgaaac gagtcaaacg
    51901 aaggttacag tcagccataa tcgtttagac gtgtataaac gtcgtcctga cacattaaaa
    51961 aaaggagcgg agcttctacg actagaaaca gaaaaatata tcatgaatgt ttacctagaa
    52021 aggagtgagc tggtcgaaaa ctttgtcagc tacacgattt gcaggaaaca ctattactca
    52081 gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga aatggaagag
    52141 ctaaacagca aagatgcgac ggtagacgat tattaccttg tatttactag atttttgacg
    52201 tgtgggacgg aatctatcgt gttaatggga atggattatg aactcactgt acccgaagca
    52261 tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta cacgacttta
    52321 actgattttt tagacaccta cacttacgac gatacagaag aaatccaaaa tgaacttgac
    52381 cgtaaacgaa tgccatatca acgatttgaa aggaaaaaag gtcaataacc atttataccg
    52441 aaaagtgagg atgtgtgaaa taagaacaaa aaaccttgaa attaagggaa tggttcttat
    52501 ttcgcaccaa tcgatccaac cgcccccgcc gcttaggtgg tgcgaaataa gcgggcaaag
    52561 cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct ctattgttcg
    52621 tactgtttgt tggcgagata cttatgcgtc atggtgcagc tctacattta aacttcgtag
    52681 tattcttagg aatactggta ttttgtttga tcttttggcg ctttgtgtta acccttgtga
    52741 gtctaatatt caagttgatt gcagccatat ttttaatagg gttatttcga tgaaaggaga
    52801 gagtactaat gccaattgca ataggaaata aacggttacc cgtgacgttg gatgaaaaga
    52861 gacaaaaaga attgcagcaa ctaaagcaga agtacggcaa aagtgaatcc aggattatgt
    52921 gtattgcgtt agatttattg attgcccaag aaaaagcagg atttgaggta ccagcactca
    52981 aaaagtgacg tcacttttta tcctaaaaac taaaagtgat agcactttta acagaattag
    53041 gcagaagtcg cccatcctct ataggtgggt gatgaatgcc gttcggtatg agggttaccg
    53101 ttggtaactc gaagaccgac aactaaggcg tattgccttt ttattttgt
//
LOCUS       DAJSBN010000016        48953 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJSBN010000016 DAJSBN010000000
VERSION     DAJSBN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.016"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            591..1280
                     /locus_tag="ONT86_001037"
     CDS             591..1280
                     /locus_tag="ONT86_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2351846.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ONT86_001038"
     CDS             1267..2475
                     /locus_tag="ONT86_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2351847.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ONT86_001039"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ONT86_001039"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR2351848.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ONT86_001040"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ONT86_001040"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR2351849.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ONT86_001041"
     CDS             complement(6578..7273)
                     /locus_tag="ONT86_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCR2351850.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ONT86_001042"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ONT86_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR2351851.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ONT86_001043"
     CDS             complement(8472..8663)
                     /locus_tag="ONT86_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCR2351852.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ONT86_001044"
     CDS             complement(8684..9574)
                     /locus_tag="ONT86_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR2351853.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ONT86_001045"
     CDS             complement(9561..10328)
                     /locus_tag="ONT86_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2351854.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ONT86_001046"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ONT86_001046"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR2351855.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ONT86_001047"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ONT86_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR2351856.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ONT86_001048"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ONT86_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR2351857.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ONT86_001049"
     CDS             14899..15282
                     /locus_tag="ONT86_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351858.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="ONT86_001050"
     CDS             complement(15450..16208)
                     /locus_tag="ONT86_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCR2351859.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ONT86_001051"
     CDS             complement(16226..17044)
                     /locus_tag="ONT86_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR2351860.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ONT86_001052"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ONT86_001052"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR2351861.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ONT86_001053"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ONT86_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR2351862.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ONT86_001054"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ONT86_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR2351863.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ONT86_001055"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ONT86_001055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR2351864.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ONT86_001056"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ONT86_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCR2351865.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ONT86_001057"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ONT86_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR2351866.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ONT86_001058"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ONT86_001058"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR2351867.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ONT86_001059"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ONT86_001059"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCR2351868.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="ONT86_001060"
     CDS             27086..27385
                     /locus_tag="ONT86_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR2351869.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ONT86_001061"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ONT86_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2351870.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="ONT86_001062"
     CDS             27973..28209
                     /locus_tag="ONT86_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR2351871.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ONT86_001063"
     CDS             28352..30325
                     /locus_tag="ONT86_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCR2351872.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ONT86_001064"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ONT86_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR2351873.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ONT86_001065"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ONT86_001065"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR2351874.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ONT86_001066"
     CDS             33204..34496
                     /locus_tag="ONT86_001066"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR2351875.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35232..36095
                     /locus_tag="ONT86_001067"
     CDS             35232..36095
                     /locus_tag="ONT86_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2351876.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36224..37846)
                     /locus_tag="ONT86_001068"
     CDS             complement(36224..37846)
                     /locus_tag="ONT86_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2351877.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38095..38616
                     /locus_tag="ONT86_001069"
     CDS             38095..38616
                     /locus_tag="ONT86_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR2351878.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="ONT86_001070"
     CDS             complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="ONT86_001070"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCR2351879.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40697..41515
                     /locus_tag="ONT86_001071"
     CDS             40697..41515
                     /locus_tag="ONT86_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCR2351880.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41618..41815)
                     /locus_tag="ONT86_001072"
     CDS             complement(41618..41815)
                     /locus_tag="ONT86_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351881.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41984..42562
                     /locus_tag="ONT86_001073"
     CDS             41984..42562
                     /locus_tag="ONT86_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR2351882.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42657..42794
                     /locus_tag="ONT86_001074"
     CDS             42657..42794
                     /locus_tag="ONT86_001074"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351883.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43607..44110)
                     /locus_tag="ONT86_001075"
     CDS             complement(43607..44110)
                     /locus_tag="ONT86_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR2351884.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44469..45494
                     /locus_tag="ONT86_001076"
     CDS             44469..45494
                     /locus_tag="ONT86_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2351885.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45817..46695
                     /locus_tag="ONT86_001077"
     CDS             45817..46695
                     /locus_tag="ONT86_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCR2351886.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47171..47287
                     /locus_tag="ONT86_001078"
     CDS             47171..47287
                     /locus_tag="ONT86_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR2351887.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48215..48553
                     /locus_tag="ONT86_001079"
     CDS             48215..48553
                     /locus_tag="ONT86_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351888.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48647..48877
                     /locus_tag="ONT86_001080"
     CDS             48647..48877
                     /locus_tag="ONT86_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351889.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT        15092 a         9132 c         9584 g        15145 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaacaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
    34681 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
    34741 ctttttcaca acctctccac taaacggtgt tctcctgcca actcctcggt cacaagtcac
    34801 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctaaa
    34861 cggcaggctc acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
    34921 aaaaaacggc aaaatatgag gaactatttt ttccgttttt ttctctaata ctcaggtcaa
    34981 aacgctgatt tcacaacctc tgattcacgg tgttccagaa gtagcttcta cggaaataag
    35041 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    35101 tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa accggttaaa
    35161 gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt aacgagaagg
    35221 ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca taccatgtat
    35281 tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat caagagttag
    35341 tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg aaaaatggtg
    35401 cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc cgttcttatg
    35461 cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc ggctcttaca
    35521 gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct gagatctatg
    35581 tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa gctgtaagat
    35641 tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt aattatttga
    35701 aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc ggtaggattt
    35761 ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt acggtggata
    35821 aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg atccagacaa
    35881 tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt tatgtggctt
    35941 acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag cagataaaga
    36001 tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca ggctatggct
    36061 gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa
    36121 gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag catcctatct
    36181 cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc cataactcgt
    36241 tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta tcagctcgat
    36301 cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg ataaattgat
    36361 ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca ttcaatgctg
    36421 tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg ttagctttcg
    36481 aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa acattcactg
    36541 gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt
    36601 tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt agatcagtat
    36661 cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca ccccaacgta
    36721 ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt gttgttgtta
    36781 tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa gatatatcat
    36841 ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat aagtcgtttc
    36901 caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa ggttggatat
    36961 cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt gattttgatg
    37021 cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct tcttttttgg
    37081 catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa aaatcataat
    37141 ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta catactttgt
    37201 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
    37261 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
    37321 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
    37381 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
    37441 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
    37501 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
    37561 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
    37621 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
    37681 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
    37741 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
    37801 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
    37861 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
    37921 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
    37981 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
    38041 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
    38101 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
    38161 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
    38221 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
    38281 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
    38341 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
    38401 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
    38461 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
    38521 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
    38581 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
    38641 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
    38701 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
    38761 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
    38821 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
    38881 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
    38941 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
    39001 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
    39061 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
    39121 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
    39181 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
    39241 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
    39301 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
    39361 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
    39421 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
    39481 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
    39541 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
    39601 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
    39661 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
    39721 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
    39781 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
    39841 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
    39901 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
    39961 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
    40021 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
    40081 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
    40141 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
    40201 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
    40261 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
    40321 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
    40381 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
    40441 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
    40501 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
    40561 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
    40621 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
    40681 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
    40741 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
    40801 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
    40861 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
    40921 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
    40981 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
    41041 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
    41101 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
    41161 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
    41221 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
    41281 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
    41341 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
    41401 ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt ttgcagtttt
    41461 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
    41521 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
    41581 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
    41641 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
    41701 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
    41761 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
    41821 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
    41881 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
    41941 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
    42001 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
    42061 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
    42121 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
    42181 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
    42241 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
    42301 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
    42361 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
    42421 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
    42481 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
    42541 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
    42601 aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg
    42661 atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg
    42721 atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag
    42781 caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt
    42841 ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga
    42901 aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg taagagataa
    42961 aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga tatacaacag
    43021 aaatgaataa atattaacgt gttttcaaac agtataaaac ggtgtggttt ttggaataaa
    43081 aaaataacat atctcatttt acgcttttgt tattttatac ttaaaaaata acgacacaga
    43141 aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt tcctttgaaa
    43201 tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga agggagccta
    43261 aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag ctttttatag
    43321 aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta gattgagata
    43381 ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc ctttttgtta
    43441 aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc aataatatag
    43501 tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag accgtcatag
    43561 atacacattg gaatgtgtct attacggtct ttttttacga acgattttat tgaagacgga
    43621 cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg tatcccaaaa
    43681 taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt tgataggccc
    43741 ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc gctacgccaa
    43801 taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata atccctaatt
    43861 ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga
    43921 caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa
    43981 aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata
    44041 gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa aattttgata
    44101 cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc
    44161 ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta gaagcttttt
    44221 tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc
    44281 tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt
    44341 gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg
    44401 ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga
    44461 gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta
    44521 tcatgaatct gatgctactt atctttattg tattatggtt aggttacata aatcatgatt
    44581 ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat caaaacatac
    44641 cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg
    44701 ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg
    44761 ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt
    44821 ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg
    44881 tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat
    44941 tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg
    45001 aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt
    45061 atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga
    45121 ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat
    45181 cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga
    45241 ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg
    45301 cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag
    45361 catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg
    45421 gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc
    45481 aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc
    45541 acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca
    45601 aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttgttt
    45661 atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa agtcttacag
    45721 atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa agtatgttta
    45781 taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga tatgaaaagg
    45841 ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac tttaactctt
    45901 cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga tgaggtgttt
    45961 gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc aaagaaaata
    46021 gacaaagtct atgaagagga accttttgca gcattagcta tttatctata tgcagcttct
    46081 caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta tttggcaaca
    46141 atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag ctatcgcttt
    46201 gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat tggtgaattg
    46261 gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga aggaaagcag
    46321 tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat aagagatagt
    46381 tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta ccaagtgatg
    46441 tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa agagtcaaaa
    46501 atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga cgatagtgga
    46561 gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga aagtacgagt
    46621 ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc tggcttaggt
    46681 attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa
    46741 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt
    46801 cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt
    46861 tcttggagtt aaacgcttct gtcccagcct cttttttagt gttctatcga acacacagta
    46921 cgttcaatag gtaaactagc tataacaata gatattaaag ttaacggaca agagggcctt
    46981 ttttaggtag ctaaaacgta ataatgaaaa tttatttcca gagataggag tcaatcagct
    47041 agtctgatcg actgttttaa caagttgact ttcattttaa cttttgatac catttaaaac
    47101 acaaatcgta attgtttcga tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg
    47161 ggtcgtccat atggaaaaaa cagatatctc aagtgcatac cgccgcttga aaagcccaaa
    47221 tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga atagtgggaa
    47281 aaggtaagaa gaaggaaact taattaaaaa aatagccgaa aaacgttttt cggctatttt
    47341 caataatcgg tttttgtgta aggtttccga aagaactgct gttgattttc catttttaac
    47401 atatgataaa ttggtagaat ctttcttttc aaattgatac cattccctaa ttccagttat
    47461 atagttaact acagaaccac aaagcacgta cgtttataaa agtattatct gtagttattt
    47521 ttcaattccc tttcaacctc ttattttgac cagtagtatt gatcacttta ccttttaaca
    47581 cctttgttcg tttttgaaac atttttagtc aagaatggaa acgtaactac ataatcaaag
    47641 gttttgcgaa atatttcatg aaatatttca tgtagaagcg agcaatacgg ttgcaaataa
    47701 ccattatcgt ttaataagca gttacgaaag ctcccaatat ctctaacgtt cagctcactc
    47761 catttctttt cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc gaaaaagtta
    47821 actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt actattgcta
    47881 gaataacatc aaggaataat aagttttggt tttcagataa tagtcgataa gttgtgtatt
    47941 ggaattttag atacagtaat aaagtatttt tcatgaaatt ttgtgagaat cactattaca
    48001 agtatcttta ataaaattaa tggttaagga ggaacttaaa gtagaatgta aatgaatagt
    48061 tgtagtcagt cgattgtgat actagactca aagttgcagg aataagtttt tcaatttgtg
    48121 attggaagat aaaaaatgaa aaagaagggc ttttttttat actttgttga ttgtagtgtt
    48181 tattttccct ctgtttagcc aagtaacatt ttctatgact caaccagttt caagtgagga
    48241 acatgataac ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa atattgttcc
    48301 ggagactcca ttagctagtg agccaattac tgatattgat ggaaaccaaa taggatggta
    48361 tatacagtac gaagttatgg tggtaaataa tgactcaata gtaaaaaata cagtaggctt
    48421 agagctaaca ccgattgaaa aggtgagttc attgttaacg tatatttata ctgatgaaag
    48481 tgacgagact gagatcattt taacggatta ctcaaaaatt gctactgata aaaagtatat
    48541 attgtggatg tagacactag tgaaatactt tatgatatag aaattgatga aaatttacta
    48601 gatgtagcac aagaaatcga tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa
    48661 gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg
    48721 gaaagaattc aagagtcgct actcaattaa ctcgactccg acatttacag tttacagaga
    48781 aggatcgata gaaaaaactg ttttttggac taaagaaagt ggaatgtctt tagctgaagt
    48841 agaagagttt ttggattatg ttagtatgca acagtaaggg agttcttatt atgaaaaatt
    48901 ttgacacata ttatgttatt gttttttcga tatctttgct gttaatatta cta
//
LOCUS       DAJSBN010000017        47952 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJSBN010000017 DAJSBN010000000
VERSION     DAJSBN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.017"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(38..1189)
                     /locus_tag="ONT86_001081"
     CDS             complement(38..1189)
                     /locus_tag="ONT86_001081"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR2351890.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ONT86_001082"
     CDS             complement(1202..1639)
                     /locus_tag="ONT86_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2351891.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ONT86_001083"
     CDS             complement(1654..3420)
                     /locus_tag="ONT86_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR2351892.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ONT86_001084"
     CDS             3684..4361
                     /locus_tag="ONT86_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2351893.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ONT86_001085"
     CDS             4364..5491
                     /locus_tag="ONT86_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2351894.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ONT86_001086"
     CDS             complement(5691..6131)
                     /locus_tag="ONT86_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2351895.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ONT86_001087"
     CDS             complement(6142..8175)
                     /locus_tag="ONT86_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2351896.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ONT86_001088"
     CDS             complement(8177..9880)
                     /locus_tag="ONT86_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2351897.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ONT86_001089"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ONT86_001089"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCR2351898.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ONT86_001090"
     CDS             complement(10963..11988)
                     /locus_tag="ONT86_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR2351899.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ONT86_001091"
     CDS             complement(12197..13066)
                     /locus_tag="ONT86_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCR2351900.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ONT86_001092"
     CDS             complement(13525..15417)
                     /locus_tag="ONT86_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2351901.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ONT86_001093"
     CDS             complement(15414..17141)
                     /locus_tag="ONT86_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2351902.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ONT86_001094"
     CDS             complement(17157..17807)
                     /locus_tag="ONT86_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR2351903.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ONT86_001095"
     CDS             18011..18307
                     /locus_tag="ONT86_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR2351904.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ONT86_001096"
     CDS             18479..19099
                     /locus_tag="ONT86_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR2351905.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ONT86_001097"
     CDS             complement(19191..20357)
                     /locus_tag="ONT86_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2351906.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ONT86_001098"
     CDS             complement(20369..20722)
                     /locus_tag="ONT86_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR2351907.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ONT86_001099"
     CDS             20850..21872
                     /locus_tag="ONT86_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCR2351908.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ONT86_001100"
     CDS             21891..22052
                     /locus_tag="ONT86_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351909.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ONT86_001101"
     CDS             complement(22311..22745)
                     /locus_tag="ONT86_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351910.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ONT86_001102"
     CDS             complement(22855..24714)
                     /locus_tag="ONT86_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR2351911.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="ONT86_001103"
     CDS             complement(24832..25992)
                     /locus_tag="ONT86_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HCR2351912.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ONT86_001104"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ONT86_001104"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR2351913.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ONT86_001105"
     CDS             complement(27739..28437)
                     /locus_tag="ONT86_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR2351914.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ONT86_001106"
     CDS             complement(28394..30055)
                     /locus_tag="ONT86_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCR2351915.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ONT86_001107"
     CDS             30273..31673
                     /locus_tag="ONT86_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCR2351916.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ONT86_001108"
     CDS             complement(31925..33004)
                     /locus_tag="ONT86_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2351917.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ONT86_001109"
     CDS             complement(33090..34049)
                     /locus_tag="ONT86_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR2351918.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ONT86_001110"
     CDS             complement(34061..35572)
                     /locus_tag="ONT86_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR2351919.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ONT86_001111"
     CDS             complement(35594..36157)
                     /locus_tag="ONT86_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR2351920.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ONT86_001112"
     CDS             complement(36163..37026)
                     /locus_tag="ONT86_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2351921.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ONT86_001113"
     CDS             complement(37040..37936)
                     /locus_tag="ONT86_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2351922.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ONT86_001114"
     CDS             complement(38111..39400)
                     /locus_tag="ONT86_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2351923.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ONT86_001115"
     CDS             complement(39372..40889)
                     /locus_tag="ONT86_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR2351924.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="ONT86_001116"
     CDS             41087..41977
                     /locus_tag="ONT86_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR2351925.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ONT86_001117"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ONT86_001117"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR2351926.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="ONT86_001118"
     CDS             complement(42886..44358)
                     /locus_tag="ONT86_001118"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR2351927.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44995..45357)
                     /locus_tag="ONT86_001119"
     CDS             complement(44995..45357)
                     /locus_tag="ONT86_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR2351928.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45580..46332
                     /locus_tag="ONT86_001120"
     CDS             45580..46332
                     /locus_tag="ONT86_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2351929.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46472..47395)
                     /locus_tag="ONT86_001121"
     CDS             complement(46472..47395)
                     /locus_tag="ONT86_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2351930.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47410..47682)
                     /locus_tag="ONT86_001122"
     CDS             complement(47410..47682)
                     /locus_tag="ONT86_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351931.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13979 a         9954 c         8268 g        15751 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttttt
    44941 attgtataca ttagatatgg agcaggttac cataaagatg attattagtc gttattaaat
    45001 agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac gtttgaattg
    45061 tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat cttctcgtgt
    45121 catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt
    45181 attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt gtgttcgatc
    45241 taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt ccactacttt
    45301 aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta gcttcatctc
    45361 attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac catctttcct
    45421 agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac cttttatcac
    45481 ttaatagtat aacataattt catgagaaaa aaatacaatt ttttcttgac catctcgtta
    45541 cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac aataaagaaa
    45601 atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga gattggactg
    45661 ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa agaagtcgat
    45721 cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga tatccaagag
    45781 gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca gcaaatgttg
    45841 ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac acttgatatg
    45901 tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag
    45961 ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag
    46021 gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga agaaatgcaa
    46081 actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc agaagatgaa
    46141 agcttagcag accgtatctt ccaagctcat aagaagtggt tatcttacag ctggcctgaa
    46201 tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga acggttcact
    46261 gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga aattatccaa
    46321 aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg acctaataac
    46381 aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc tccattcttt
    46441 cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta taaggtattt
    46501 tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg caagcttcca
    46561 gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa
    46621 tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca accatatccg
    46681 aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct tgttttcctt
    46741 tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga aaaagttgat
    46801 gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc cccaacgtga
    46861 tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta attgattgtt
    46921 ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa aaaactagag
    46981 gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac gtaatgatca
    47041 atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt tctttctgat
    47101 agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca atgatcccac
    47161 tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca atgcttttcg
    47221 tttttccata acttaactgt ctggcatttg cattagggat ataatcgact tctttcatga
    47281 ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg attacacgtg
    47341 aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt gccactgctg
    47401 cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg gaccgcattc
    47461 aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc cccaatatat
    47521 atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat tcctattcaa
    47581 cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg cataaaaata
    47641 attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc ctccttttga
    47701 tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt gccaaagaaa
    47761 agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat acattataat
    47821 gatactgtaa atatatcttt attattagtg aatggatatt tacttttaat agataaaata
    47881 ttataactat taaaaataac tattttcatt ataattacac acaaaaaaga gcgtgagaca
    47941 aaagtaaaga ac
//
LOCUS       DAJSBN010000018        47855 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJSBN010000018 DAJSBN010000000
VERSION     DAJSBN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.018"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            176..2341
                     /gene="acm"
                     /locus_tag="ONT86_001123"
     CDS             176..2341
                     /gene="acm"
                     /locus_tag="ONT86_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HCR2351932.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2613..3098)
                     /locus_tag="ONT86_001124"
     CDS             complement(2613..3098)
                     /locus_tag="ONT86_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCR2351933.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3188..3533
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3578..4219
                     /locus_tag="ONT86_001125"
     CDS             3578..4219
                     /locus_tag="ONT86_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2351934.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4238..4873
                     /locus_tag="ONT86_001126"
     CDS             4238..4873
                     /locus_tag="ONT86_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2351935.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4891..5706
                     /locus_tag="ONT86_001127"
     CDS             4891..5706
                     /locus_tag="ONT86_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2351936.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6025..7461
                     /locus_tag="ONT86_001128"
     CDS             6025..7461
                     /locus_tag="ONT86_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2351937.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7650..10097
                     /locus_tag="ONT86_001129"
     CDS             7650..10097
                     /locus_tag="ONT86_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR2351938.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10327..11394
                     /gene="rlmN"
                     /locus_tag="ONT86_001130"
     CDS             10327..11394
                     /gene="rlmN"
                     /locus_tag="ONT86_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCR2351939.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11587..12690)
                     /locus_tag="ONT86_001131"
     CDS             complement(11587..12690)
                     /locus_tag="ONT86_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR2351940.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13243..13791
                     /gene="aac(6')"
                     /locus_tag="ONT86_001132"
     CDS             13243..13791
                     /gene="aac(6')"
                     /locus_tag="ONT86_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR2351941.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14337..15998
                     /locus_tag="ONT86_001133"
     CDS             14337..15998
                     /locus_tag="ONT86_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2351942.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(16068..16415)
                     /locus_tag="ONT86_001134"
     CDS             complement(16068..16415)
                     /locus_tag="ONT86_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCR2351943.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16499..17440
                     /locus_tag="ONT86_001135"
     CDS             16499..17440
                     /locus_tag="ONT86_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2351944.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17441..18484
                     /locus_tag="ONT86_001136"
     CDS             17441..18484
                     /locus_tag="ONT86_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2351945.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18490..19560
                     /locus_tag="ONT86_001137"
     CDS             18490..19560
                     /locus_tag="ONT86_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2351946.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19550..20503
                     /locus_tag="ONT86_001138"
     CDS             19550..20503
                     /locus_tag="ONT86_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2351947.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20515..21525
                     /locus_tag="ONT86_001139"
     CDS             20515..21525
                     /locus_tag="ONT86_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2351948.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21810..22631)
                     /locus_tag="ONT86_001140"
     CDS             complement(21810..22631)
                     /locus_tag="ONT86_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR2351949.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22728..23006)
                     /locus_tag="ONT86_001141"
     CDS             complement(22728..23006)
                     /locus_tag="ONT86_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351950.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23274..25283
                     /gene="metG"
                     /locus_tag="ONT86_001142"
     CDS             23274..25283
                     /gene="metG"
                     /locus_tag="ONT86_001142"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR2351951.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25762..26349)
                     /locus_tag="ONT86_001143"
     CDS             complement(25762..26349)
                     /locus_tag="ONT86_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351952.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26524..27846
                     /locus_tag="ONT86_001144"
     CDS             26524..27846
                     /locus_tag="ONT86_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2351953.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            28001..28777
                     /locus_tag="ONT86_001145"
     CDS             28001..28777
                     /locus_tag="ONT86_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR2351954.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28770..29339
                     /gene="rnmV"
                     /locus_tag="ONT86_001146"
     CDS             28770..29339
                     /gene="rnmV"
                     /locus_tag="ONT86_001146"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCR2351955.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29332..30216
                     /gene="rsmA"
                     /locus_tag="ONT86_001147"
     CDS             29332..30216
                     /gene="rsmA"
                     /locus_tag="ONT86_001147"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR2351956.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30667..31296
                     /locus_tag="ONT86_001148"
     CDS             30667..31296
                     /locus_tag="ONT86_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351957.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31420..32529)
                     /gene="ugpC"
                     /locus_tag="ONT86_001149"
     CDS             complement(31420..32529)
                     /gene="ugpC"
                     /locus_tag="ONT86_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCR2351958.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32738..33160
                     /gene="mgsA"
                     /locus_tag="ONT86_001150"
     CDS             32738..33160
                     /gene="mgsA"
                     /locus_tag="ONT86_001150"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR2351959.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            33483..34019
                     /locus_tag="ONT86_001151"
     CDS             33483..34019
                     /locus_tag="ONT86_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR2351960.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(34059..35822)
                     /locus_tag="ONT86_001152"
     CDS             complement(34059..35822)
                     /locus_tag="ONT86_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2351961.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(35825..37540)
                     /locus_tag="ONT86_001153"
     CDS             complement(35825..37540)
                     /locus_tag="ONT86_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2351962.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(38319..38543)
                     /locus_tag="ONT86_001154"
     CDS             complement(38319..38543)
                     /locus_tag="ONT86_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351963.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            38734..41511
                     /locus_tag="ONT86_001155"
     CDS             38734..41511
                     /locus_tag="ONT86_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2351964.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            41592..42011
                     /locus_tag="ONT86_001156"
     CDS             41592..42011
                     /locus_tag="ONT86_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCR2351965.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            42023..42493
                     /locus_tag="ONT86_001157"
     CDS             42023..42493
                     /locus_tag="ONT86_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2351966.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            42510..43289
                     /locus_tag="ONT86_001158"
     CDS             42510..43289
                     /locus_tag="ONT86_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2351967.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            43276..44097
                     /locus_tag="ONT86_001159"
     CDS             43276..44097
                     /locus_tag="ONT86_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2351968.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            44114..45163
                     /locus_tag="ONT86_001160"
     CDS             44114..45163
                     /locus_tag="ONT86_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2351969.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            45176..46180
                     /locus_tag="ONT86_001161"
     CDS             45176..46180
                     /locus_tag="ONT86_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2351970.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            46813..47832
                     /locus_tag="ONT86_001162"
     CDS             46813..47832
                     /locus_tag="ONT86_001162"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR2351971.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        15850 a         8311 c         9877 g        13817 t
ORIGIN      
        1 aaaaaccttg taaaaacagt tttttggcat atttattttt tataaatatt tgtaaaataa
       61 ctatcaattt tttagttaaa agattggaaa tgagtggggg aggaattgaa gaaaggattt
      121 ttgagagatg atatagtagg ttttattttt agttaaaata ggggagatag aaaaaatgaa
      181 aaagtgtaga aatattattt tatcaatgtt atttattatt actaatataa catcactgat
      241 tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac
      301 cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga
      361 aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa
      421 tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg
      481 aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct
      541 agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg
      601 tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt
      661 gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct
      721 acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt
      781 tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac
      841 gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat
      901 ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta
      961 cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa
     1021 gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa
     1081 agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc
     1141 agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa
     1201 tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc
     1261 tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa
     1321 aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt
     1381 tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt
     1441 cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa
     1501 cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa
     1561 ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac
     1621 ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga
     1681 agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct
     1741 agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga
     1801 caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg
     1861 tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa
     1921 tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc
     1981 cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac
     2041 aaatatatct ataccaggta gccatataac accgtggaaa aattttccag agaagacaga
     2101 gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga
     2161 atccagcgaa caaagaagca attatattag taagagagct gtacctaaac agataacaaa
     2221 taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat
     2281 tggtttattg atagtaacta tctgtgctgg gatattctta caaaaatata aaaaagttta
     2341 gttagaatat aatatttttt gttaaatatg actgaaatca aaagaataaa attccgattt
     2401 ataatcgaaa aaagaagcat ttttatatat gcttcttttt tcgtagaatc aataaatatg
     2461 gatttggcta actatggaag aatactcgta ttagttttca gcgttcatac tagttgttac
     2521 attgtttgat tggccaaaag aatttctgat atattttgtg ctaaataaag aaaaagacgc
     2581 ttgaattggg aaataagaaa actactctat cattacaaat tttcataaac tggcagatgt
     2641 tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt agtggtattg
     2701 atatttggat gaaagcctac ggtatcttga cgatcaatag cagcatcgat cacgacttgg
     2761 atttttttct ctttgtcgtg catcaatgct aatggagtca ctgtacctgc tggaacgcct
     2821 aggaggtcag gaagctgttc ttcagaaaca aaagataagc gcttttcttt taattgttca
     2881 gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga tgacaagata gatttgtttt
     2941 ccttttcttg atttcaataa tagatttttt acttgcggac caggtaggga aaaagggaca
     3001 tttttgactg acgtaatcgc aggatgatcg actttttgaa aagagatacc cagttttttt
     3061 agtagttcat aagcttcttg ttcggttgca cgactcatcg cgcatcaccc tccttttttt
     3121 attagtgtat cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat tacacgtaaa
     3181 gttaaaacaa atgacatcga aaagaagagt agccgttagg tgtttttaga gagtctctgg
     3241 ttggtggaaa gaggcagcag ttaaccgtga aacacatctt ggagtcgatt ttctgaagcg
     3301 tatagtagga aaatccgggg agcccgttac agctgcggtc gttgctagat gaccagataa
     3361 ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt aacgtccttt
     3421 gatcttcgga tcaaaggact ttttgttttc atctgaacgg caacgactgt gtgaggtttt
     3481 ttctgcaagg aaaaaataaa cagaggtgga accacgtata atacgtcctc ttggcatttg
     3541 ccaagaggat ttttttattt taaaggagga aaagaagatg gagtatttga ttgaagttat
     3601 gccgcaatta ttagtaggag cactcacaac attgaaatta tttttattta ccttgattgg
     3661 ctcattacca ttagggattg ttttagcatt aggattgtct ttgcatttta agccgctacg
     3721 ctacttgctt caaacatatg tatgggttat gcggggaaca ccgctgttgt tgcaactgat
     3781 ttttattttt tatgggttgc cgacgattgg gatcgttttt gatcgtttcg aagcagcact
     3841 cattgccttt atcttaaact atgcagctta ttttgcagag atctttcgtg gagggataca
     3901 atcgattcct caaggtcaat atgaagcagc gcaagttttg caattaagtc cctttcaaac
     3961 ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt ttaccttctg tagggaatga
     4021 agttatcaac ttggtcaaag attcttcctt gatctatata ttaggtttag gtgacgtaat
     4081 gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc taggcgtggc
     4141 aatcatctat cttacattga ctggtattct aacaatcata atgaaacaag ttgaaggaaa
     4201 attaaattat taccgctaac tgataggagg aagaaatatg attgaattga agaaagtgaa
     4261 caaagcattt ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc cagatgggaa
     4321 aacattagct atcgtaggtc cttccggtgg cggtaaaacc acgcttttac gtatcttggc
     4381 tggcttagaa agttctgaca gcggagaatt ttatttagac ggtgtaccgt tcaacccatt
     4441 cgagcttaaa atgaaagaac aagttgtagg agtcgtattc caagacttcc agctatttcc
     4501 acatctgaat atttttgaaa acattacctt agcaccaaaa atggtattga aacagacaaa
     4561 agaagaatac accaaaaaag cagaagaatt agcggcttca ttagggatta gtgatttatt
     4621 gaatatctat ccttatcagc tttctggcgg acaaaaacag cgggtagcga ttgccagagc
     4681 attggctatg aatccacggg tattggctta tgatgaacca acaagtgctt tagatcctga
     4741 attacgccag caagtcgagc gtttgatcct atcattgaaa gaagatgggg taacccaaat
     4801 tattgtcacc catgatttag tatttgcaga aaatgtcggt gatcaattac taaaagtttc
     4861 cccaattcat taagaaagga gaagatagag atgaaaaaaa ccagttttct attcgctttg
     4921 atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc agataattgg
     4981 gcgactatca acgaagagaa aaaaatcgtt ataggtttag acgatacatt tgttcctatg
     5041 ggatttagag ataaagacgg gaagctcaca ggatttgata ttgatttagc caaagcagtc
     5101 tttgaacaat atgggattac ggtagacttt cagcccattg actggtcaat gaaggaattt
     5161 gaactgaaca atggaacgat cgatttgata tggaacggct attccaaaac gccggcaaga
     5221 gaaaagaaag tgcaatttac aaaaccttat atggaaaacg atcaagtgct gatcacacct
     5281 aaagaatcag gaatcacaaa ctttggacaa atgaataata agagattagg tgcgcaaaat
     5341 gggtcatctg gttatgatgt atttacgaaa caaccagaag tattaaaaga gatagtcaaa
     5401 aaccaagatg ctgttttgta tgatagtttt aatgaagcat tgatcgatct gaaaagcaga
     5461 cgggtcgatg gtctgctgat ggataaagta tacgcagact attatttgaa acaaagaaac
     5521 gaactagaag acttcaacat aacaaaaggt tcttatgaga gtgaggattt tgcagttggt
     5581 gcacgcaagt cagatagtac attagtgaaa aaaatcaatg aagccctaga tcaactagag
     5641 aaaacaggtg aattccaaaa aatatccgat aaatggtttg gcgaagatgt tacgccgaga
     5701 aattaacaaa taggggactg tgacaaaaga cgtgtcacag tccctttttc cgaataaaca
     5761 gtgttctcct gccaactcct tgtgactcgg agctgaatgg caggctcaca acctcttggt
     5821 aaacggtgtt caaaagccag tttttcggta ttaagccaaa tttatgcaaa aatgtagaaa
     5881 gccattttca caaaattttt cttactactc gaaactaagc gactttttca caacctcctt
     5941 tacataattt tattttgttg tggaaggggt ttcgtgttat tctaaaagag gaacgattga
     6001 aggttatagg aaaggaagag atgaatggaa cacaaagaac ttacctcatt tccaaaagat
     6061 tttctgtggg gctctgcttc tgcagcgtat caagtagaag gagcttggca agaagatggg
     6121 aaaggagaat cagtctggga tcgatttgta cggatacctg gaaagacatt taaaggaacg
     6181 aacggtgatt tggcagtaga ccactatcat cgttataaag aagatattgc tttaatgaaa
     6241 gaacaaggtc tgaaagctta ccgcttctct gtagcttgga cacggatttt tcctaatgga
     6301 cgaggagaag tcaatcaagc tgggctcgct ttttatgaga gattgatcga cgaattgatt
     6361 gaaaacgata ttgaacctat gttgacttta tatcattggg atcttccaca agcattgcaa
     6421 gatgaatata atggttggga atcacgtcag atcattgaag atttcactca ttatgcagaa
     6481 acattatttg aggcattccg cgggaaagtc cattactgga ttagtttgaa tgaacaaaat
     6541 atcttcactt cattaggtta tttgttagca gcacatccgc caggtgtgac agacccgaaa
     6601 cgaatgtatg aagttaacca tattgctaat ttagcaaatg catctgtcat caataaattt
     6661 cacgagttag aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc aaattatcca
     6721 attgacagta atcctgaaaa tgttctggct gctgaaaatg cagaagacct gatggccaat
     6781 tattggatgg atgtctatat gtggggaaaa tatccgattg ctgccatgag gttcttagaa
     6841 gaaaaaggat gggcaccaac gattgaagct ggcgatgcag aattgctaga atctgctaaa
     6901 ccagattttc ttgggatcaa ttactatcaa acggcgacga atgcattcaa tccattaaac
     6961 ggagttggtg ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga agaaactgga
     7021 gttcctggaa tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa ttgggactgg
     7081 gagattgacc cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg ttaccgcatg
     7141 ccggtactga ttacggaaaa tggcttagga gagtatgaca agttgacaga agataaacaa
     7201 atccatgacg actatcgaat caattactta caaagccacg tcaaagccat caaagaagca
     7261 atttcggatg gagcagaagt cctaggctat tgcacctggt cttatacaga tttgcttagc
     7321 tggctgaacg gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga cgaaacacaa
     7381 gaaggatctt tggaacgata taagaaagac agttattact ggtaccaaaa actgattgaa
     7441 gaaaatggag agaaccttta gttcacatac ttaaaacaag aaggtacggc tggatatggc
     7501 cgtgcctttt cttgataaaa tcatttagaa attaagaaaa actgtaaaat aagctgaaaa
     7561 atgaaagaaa gcgtcagttc tttcaaacaa aactttagtt cccttacttt tttttgtata
     7621 atgaaacttg atgaaatgag ggataataaa tggcacaaaa gaaacggcct actaaaaaaa
     7681 ggaaaaagac gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg ctgacgattg
     7741 gtgttgggtt tgccttgatt ttatttgctg tcttcggctt tttaaaatta ggttttttag
     7801 gaatattgat tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa atcctctgtc
     7861 ttttattagc cgtattagga ttttggattg tcattaagaa tacagagttt tcaattggta
     7921 aaaaccgacg ctggtttggc ggcatactct tttatttcgg tatcttatta ttgttacatg
     7981 cccatttatt tggcaaactc catactgggg aaccaaatat catggggacg acatgggatt
     8041 tgttagccag tgatataaaa caaagtcaag tagacaataa cgttggtggt gggatgattg
     8101 gagcaatcct ttatcatttt acttacttct tgattgcaca gcctggaagt tatttagcag
     8161 cagtgctatt actggcagga ggagcattcc tgttcagtaa tctggaaggc tatcagctgt
     8221 taaatggtat ccaatcaatt ggagaacgag tacaggaatt attggaaggt gatcctgcca
     8281 agcaagctcg aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca gaagcgaaag
     8341 aagcaaggcg cttagctgca aaagaagctg cagaaaaaga agctgcagaa tatgagaaga
     8401 aaaaagcggt gagccagcaa agggaaaata ctgctgatga atggcaggaa ccagaaaata
     8461 cagagccgga gcagctaagt tttgttccaa tcgacagttt tcaagaaaat atccatcctg
     8521 cgaatctaga gaaaccagta ccagatacac caaaacagac caatacagca gaaggttttt
     8581 cagatgagtt gccagaggat gacggtacct cattagaatt tgagattgaa gcagaacagg
     8641 aaaatcaaga ctatgaatta ccgacagttg atttgcttga ttctatacca actgtggatc
     8701 aaagtgatga gtataaaaaa attgaaaaaa atatcggcgt attagaacaa actttccaaa
     8761 gtttcggtgt cgatgccaag gtcgtcaaag ccagcttagg accatccgtc acaaaattcg
     8821 aagtccagcc ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg
     8881 cgcttgcttt agcggcgaaa gatgtccgga tggaagcacc gattccaggt aaatcactga
     8941 ttggtattga agtaccaaat ggaaagatca gtatggtttc ctttagagaa atcatcgaag
     9001 cacaaccaaa ccacccagat aaattactag aagtaccttt aggacgtgat gtttctggac
     9061 gggtacaaac ggctgatctt tcgaagatgc ctcacttact cgtggctggt tcgactggta
     9121 gtgggaagtc agtagcaatt aatgggatta tcacaagtat attaatgcgt gctaagcccc
     9181 atgaagtaaa actgatgatg atcgatccga aaatggttga attgaatatg tacaacggca
     9241 tacctcatct gttgacgccg gtcgtgacga accctagaaa agcagcgcaa gccttacaaa
     9301 aagtagtaca agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata
     9361 tttctggtta caatgaattt gttcaacaga aaaacttgga aaacggaacg aaacatccaa
     9421 ccttaccatt catcgtagtg atcgtagatg aattagctga tttgatgatg gtagccagca
     9481 atgaagttga agatgcgatt attcgtttag cgcaaatggc tcgggcagcg gggattcata
     9541 tgatcctagc tactcaacgt ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg
     9601 taccttccag aatggcattt gcggtatcaa gcggtacaga ttctcggacg atcattgatt
     9661 ctaatggggc agaaaagctc ttagggcgag gagatatgct attcttgcca atgggtgaga
     9721 ataaaccgat ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa cgggtcgttc
     9781 aatttgttac agatcagcaa gaagctcatt atgaagagaa aatgatgccg acagatgaag
     9841 ttgaaacgac aggtgctcca gaacagccac aagatgagct gtttgaagaa gcaaaagcac
     9901 ttgtcgttga aatgcaaacg gcaagtattt cccttctcca aagaagattt cggatcggtt
     9961 acaaccgagc agcacgttta gtagatgaac tggaagctca tggagttgtt ggcccgtcag
    10021 aaggaagcaa gcctcggaaa gtattcattg agcaggaaga aacatcagaa acgttgccgg
    10081 acgataatga catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa
    10141 gacaaggcat gatgatacca aaagaaaaaa tggttttttt ccatcttttc taatacggta
    10201 tgatgtcttg tctttttagt ttttgtgaga ttaaagttct attcgattta tttgtgaagt
    10261 attgcattaa ggaaatgaat catgtatagt atgtagcgaa actttgagaa aaagagggaa
    10321 aatactttgg aaaaacagtc catttatggc ttgacgaatg aagaattaat caattggttt
    10381 atagaaaacg gcgagaaaaa attccgagct gcccaagttt gggaatggtt gtatcaaaaa
    10441 cgtgtaagta atttcacaga aatgacgaat ctatcgaaac aattgatcga aaaattatcg
    10501 gagcatttta tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc ggatggtaca
    10561 gtaaaatatt tgtttgaact ccctgataaa aacatgattg agactgtact gatgagacaa
    10621 gaatatggct tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt
    10681 tgtgcaagcg ggctgctcaa aaagaaccgt gatctgactg ctggagaaat tgttgcacag
    10741 atcatgatgg tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc
    10801 gttgtaatgg ggatcggtga gccatttgat aattacgaca atgtaatgga ctttttgcat
    10861 atcatcaatg atgccaaagg attggcaatt ggtgctcggc acattacagt ttccactagt
    10921 ggattagcac acaaaatcaa ggaattcgct aataacggct tgcaagtcaa tctggctatt
    10981 tccttgcacg cgccgaataa tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc
    11041 ccaattgaga aattgatgga agctgtggat gagtatttag agaaaacaaa ccgtcgaatc
    11101 acgtttgaat atatcatgtt gaatcaagta aatgatagac cagagcatgc gcaacaatta
    11161 gcagatctat taaaagataa gaaaaaatta gcatatgtca acttgattcc atataatcca
    11221 gtcagtgaac atgatcaata ttctcgcagt ccaaaagctg atgtattgag attttatgat
    11281 gtattgaaga aaaacggcgt aaactgtgtg atacgtaaag aacatggtac agatattgat
    11341 gcagcttgcg gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat
    11401 cctactcgtt ttttcaatta ccataaaagg agcagttttc ttaattaagg aactgttcaa
    11461 cttaaataag agaaaagatt ctatagcgaa agctataaag ctttttttga caagaaaaac
    11521 cctcatcaag caaagaaatg tcctcaattt gatgacagcc tgcttgatgg gggttttagc
    11581 aattatttaa ctgattttct tatatccttg ctggctttct tgtgtagcat gataagtttc
    11641 tacgtcaaga atggcctgtt taaatttttc tcgggtgatt tcataaggca catgtttaat
    11701 atcatttgtt gccatggctt tatctaagat tgcttctaaa tcggtcgtga cattgagatt
    11761 catgtcggtt aacgatactg gaagttggtt ctttttcata aacacataga ttttatcaaa
    11821 caaatcgaac tgctgatcca tcatcaataa ggtcagtaca ccataactga cagatgctcc
    11881 gtgaaggtat ccctccattt tttctagaac agtcgtaccg taatagaatg agtgtgccat
    11941 agcagaatta tagttattgt taactaaaac agaaaccaat ccggtagtaa caatgatatc
    12001 cgaaacgact tgttcaattg catatgaggg acgatcgtcg gcactatctt tcaatgcttg
    12061 cgtaccataa gtcaagagac gatcaatgca catttttccg atttggattc ctaatctatc
    12121 cgtgtagcct agatcatcgc cacgagcaga aaaagtagat tcgacttctt tggataaagc
    12181 atcgccaata cctgcccata agtatttatc aggtgcttcg gcaataattg ctgtatcaat
    12241 gaaggtgtgt acaggtggtt ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt
    12301 atagatgaca cataccgcag tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa
    12361 gactggttta gctagttttt caccaatgac ttttacagta tcgcaagcac ggccaccacc
    12421 gactgcaaac aggacgtctg ctttttggac actttcatgc tggatcaact gttcaatatt
    12481 tccataagtt gaattacccc cgtaccagag actggcgaca acctctattt cctgtgtcaa
    12541 agctactttt attttgtttt ctgcctttga aagtgcagtc ttccctccaa tcagcactac
    12601 ttttgtccca tattctttga cgattccatt tgctttttca tacgcatctg ttccaatggt
    12661 ataatttggt aagaaaacac tttgactcat attcatttcc ttctttcttc gttgaaacgg
    12721 gttaaaattt taaagagtga tcgatctgac tgcggctgta gatggagtag aggtgaacct
    12781 aagacaggat aagtgatgaa taaggtggca tgaacacctt gaaatcatca ataagtatcc
    12841 taaaaacgaa caagtacaaa aacattgaaa acgacaataa tgaatttttg ttcctcttag
    12901 tatttctacc gattcccttt gcttttttgt aattatctaa aaattctaaa aattaagatt
    12961 attatatgaa aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat
    13021 aatctatatc atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt
    13081 tcctattttg aataaagtgt ttctgtataa tttttttatt ttataagtaa gataaataat
    13141 gaaagaatag agatactgat tggtagctaa agatttttga gcgagaacat ttcgtttttt
    13201 caaaaggact gttagaataa gcaagaacaa gggggaagac gtatgataat cagtgaattt
    13261 gaccgtaata atccagtatt gaaagatcag ctttctgatt tactgagact gacttggccg
    13321 gaagaatatg gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc
    13381 gcggtagcag cggtagacca agatgagtta gtaggattta ttggtgcaat ccctcaatac
    13441 ggtatcacag gttgggaatt gcatccatta gttgtagaaa gctcccgacg aaagaaccaa
    13501 ataggtactc gattagtcaa ttacttagaa aaagaagtag cttccagagg aggaatcacg
    13561 atttatttag gtacggatga tttagaccat ggaacaacgt taagtcaaac ggacctgtat
    13621 gagcatacat ttgataaagt ggcttccatc cagaaccttc gtgaacatcc gtatgaattc
    13681 tatgaaaaat taggttataa aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa
    13741 ccggatattt ggatggcaaa aacgattatt cctcgaccaa attctcaata ataaaaagag
    13801 gttgtgacag aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa ttacttcttg
    13861 aatttttgtg aaattccaac ttatttccga agaagctact tttggaacac cgtgtaccag
    13921 aggttgtgaa aaagtcgctt agtttcgagt agtaagaaaa attttgtgaa aatggctttc
    13981 tacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttattc
    14041 agataaggga ctgtgacaag acttttgtca cagtttcttt ttgtttattc ttttttatct
    14101 ttgtatggca ataatagaca atataacgaa aattatttaa tggcttttgg aagaccatgg
    14161 aaaaccttgt acaaagctat tttttctgaa aaattccttg aaacctcacg aaaaaatcat
    14221 tattttatga aaaagacaaa aaaaagtgtt gattttttaa taattattca ttaaactctt
    14281 aaacaacttc agaaaatatt aattattctg aaaattccaa taaaggggta cgtaaaatga
    14341 aaaagaaatt tacatttggt ttggttgcgg tatgcggcct tgtacttgct ggatgttatg
    14401 gcggcagttc agccacaagc agtagttcag caaaaagcgg ttcagcagat ggaggaggcg
    14461 tatttaactt agtcgtgcct caagaaatgc ctacagctga cttatcagtt gcaacagata
    14521 caatcagttt tacagcattg aataacgttt atgaaggaat ttaccgtctt gatgaagaca
    14581 gcaaaccaca gccggcagga gctagcgagt tggcagaagt cagtgaagac ggattaactt
    14641 acaaaatcaa attacgagaa gacgcaaaat ggtcaaatgg tgatcctgtt acagcagcgg
    14701 attacgttta tggctggcaa cgaacagtag acccagctac agcttctgaa tatgcttacc
    14761 tgtatgcgcc agttgaaaat gcagaagaca ttacagctgg aaagaaagat aaatcagaac
    14821 tagggatcaa agcagttggt gactatgaat tagaaatcaa attgacgaaa caaacaccat
    14881 atttccaata tctgcttgct ttcccatcat ttttcccaca aaaacaatcc gtagtggaag
    14941 aaaatgggga tgcctatgca tctgcaagtg ataaagctgt ttataatgga ccattcacat
    15001 tagaagattt tgatggacca ggaacagata cagaatggac ttataagaaa aatgatcatt
    15061 actgggacaa agatacagta aaactttctg aaatcaaagt cagtgtagta aaagaatctt
    15121 ctactgcatt gaacttattc aaagatggcc aagcagatga cgtgatcttg tctggtgagt
    15181 tagcacagca aaacgcaaac gatccagctt atacttctgt taaagaagcg cggacaagct
    15241 atatcgaatt caatcaacgt gaaaagaatt caccattcaa caatgtgaac ctaagaaaag
    15301 ctatttctta ttctatcgat cgtgaagctt tagtaaaaca agtactaggt gacggatcag
    15361 ttgtctctac aggattgatc ccatcagata tggtaaaaag tccaacaacg aacgaagact
    15421 ttgcaaaaga agctggaaaa ttagtttctt atgacaaaga taaagcaaaa gaatattggg
    15481 aaaaagcgaa aaaagaattg ggcgttgatt ctttagaatt tgatttgatg gcttctgatg
    15541 atgattcatc gaaaaaagta atcgaatatg ttcaaaactc aatccaagaa aacttggatg
    15601 gcgtaacagt caaaccaaca cctgttccat tctctgtacg tcttgaccgc tcaacttcgg
    15661 gtgattttga cgcagtacta ggcggatggg ctgctgacta tgcagaccca agcagcttta
    15721 ctgatttgtt tgtcacaggt aattcataca accgtggtcg ctggagcaat gcagaatatg
    15781 acgaagcagt taaagcagct tcatcaacag acgctggcga tgaacaagcc cgttgggatg
    15841 atttccaaaa agcaatcaag atcatctctg atgatatggg tgtcgttccg gtgtaccaaa
    15901 aagcagaagg tcacctagtg aatgaaaaaa tcaaaggtat cgttcaccat gcggcaggcg
    15961 cttcatggga ttacaaatgg acatacgttg aagaataatc gttaataccg tgagcatgag
    16021 aaaaaacgag gctgcggcaa atgtcgcaac ctcgttctca tattcactta tcctaacaaa
    16081 gaaaaaagca tgaaaatagc agaaagagca attagtattc cggcaatgat cagccaaaag
    16141 ctgtacatac cgtagctggc agacgagagc gaggaaaatt ctggtttttt ctttcggttc
    16201 cttgttcgtg caagatggac cgatcgctga aaatgatcga agaaacccga agaaaagacc
    16261 cataaaaaac cgccgataat cagaaacggc aatgaccata agaatagatc attagataat
    16321 tgaatgagtg taaaggaatg gttgatgaga ttttttatta aaataaccag aatcagtaga
    16381 aaagctactg cgtaggggat agattttttt ttcatttgat tgctccttta tttttggttc
    16441 attatctaat aatgtagcga aacatctgtc agaagtcaaa agctaggggg aaacattagt
    16501 gaatagttat atcaaatatg tattaaaacg tgtctttttc atgatcatca cgctttggtt
    16561 aattgcaaca atcacctttt tcttgatgca attgctgccg ggcacgccat atacgaatca
    16621 ggaacgtttg agtcctgaaa caatcgagat gctaaataaa caagtcggtt tggataaacc
    16681 agttatcgta caatatggga tttatctttc taatttgctt caaggagatt ttggaatttc
    16741 cttccaattt aaaaaccagc cagtcgcaca tttgttagct ggaagaatcg gaccatcctt
    16801 gcagttaggg ttacaggcaa ttatctttgg aacagtccta gggacgattt taggaacgat
    16861 ctctgccatg aagcaaaata catgggcaga tacatcttct acactcgtag cgatattagg
    16921 tcgttccatt ccaaactttg tatttgctgt tttattacaa tatatctttg caatcaaatt
    16981 acaagttttg ccaatcgcaa aatgggatgg ctttgtgtac acaatcttac caacaattgc
    17041 gttggccatg tcgccgcttg ctgactctgc tcggtttatc cgtacggaaa tggtggaagt
    17101 actgcatagt gattatgtgg aattagcaaa agcaaaagga ttgagccgtt gggaaatcgc
    17161 ctttaaacat ggtcttcgaa atagtttgat tccattgatg actttattag gaccgttagc
    17221 tgtagcattg atgaccggat cactggtggt agaaaatatc tttgctattc ctggtatcgg
    17281 ggaacaattc gttaagtcaa tcacaacaaa tgattatcct acgatcatgg ctgtaacgat
    17341 cctttactca tttatgctga tttttgttat tttgatcgtg gatctattgt acggtctagt
    17401 tgatccaaga attcgtgttt cagaagggag ccgaggataa atggaaatga taccaaagcg
    17461 ttatgaaaca gttgcagata tccctgcgga tgaatttctt ccattacaaa aaaataccga
    17521 agaagaacgt gaacagatta ctgcaccttc tttgaatttt atccaagatt catggcgccg
    17581 tttaaagaaa aacaaagctg ccgttatttc aatggtttta ctattggtca tcattttgat
    17641 ctctgtgatc acaatctttg tttctcctca tgatccaact gcccaaaacg tggcttatat
    17701 caatttgcct cctcgtattc cgggaatcaa tatcgatgga ttgaatggaa aagcaatggt
    17761 tggtggtgaa cttgttgata aatatgctca agcaaacgtc ccttcaaacg taaatttctt
    17821 tttaggaaca gacggtttgg gacgtgatgt attaagccgc ttatttatgg gaatgagaat
    17881 ctcgttgctg atcgcattta ttgctgcatt actagatgta acgattggtg tagcttacgg
    17941 gttggtttct ggtttgttag gcggacgtgt cgataacgtg atgcaacgtt ttttggaaat
    18001 cttatcagga attccaaacc tcgttgtcat gattttgatg ttggtcgttt ttgaaccagg
    18061 aattttttca atcgtggctg ctatggccat cacaaactgg ataccaatgg cacgtatcgt
    18121 tcgtgcacaa acaatgaaat taaaagatca agaatatgtt ttagctggat tgacattagg
    18181 tgaatcaaga atgaaaattg cattcaaaca tattttgccg aatatctcta gtgtcattat
    18241 tattcaaatg atgtttagta ttccatctgc tattttcttc gaagcctttt taagctttat
    18301 cggtttaggt cttacgcctc cgtcagcttc attaggaacg atgttgagcg aagggtacaa
    18361 gacattcctg tatctgcctt atctattatg gattccggca gtaactttat cggttatcat
    18421 gattggcttt aacttacttg ctgatggatt gcgtgatgcc tttgatccta aaatgaaaga
    18481 gtgaagacga tgacgaaaat tttagaagtg aaagacttag aaatctcttt tgatacgtat
    18541 gctggaaagg tacgagcgat tcgcggtgtc aattttcatt tgaataaagg agaaacattg
    18601 gcgatcgttg gggaatctgg aagtggaaaa tcagtgacga ccagaagcat catgcgcttg
    18661 ctgtcaagca acgccaatat agatgctggt caaatcctct ttaaaggtaa cgatatcgtt
    18721 ggaaaatcag aaagagagat gcaaaaaatc cgtggtaaag aaatagcgat gattttccaa
    18781 gatccaatga cctctttaga tccgacgatg cccattggga agcaagtcgc tgagtccctt
    18841 agaaagcata ataaagtttc aaaaaaagag ggacaaaaag ctgcattaga attgcttaaa
    18901 ttagttggaa tacccgaagc tgagaaacgt atcaacagtt atccgcatca gttctcagga
    18961 ggtcagcgtc aacggatcgt gatcgcgatt gcactggtat gttatccgga aatcctgatt
    19021 gctgatgaac ctacaacagc gcttgatgtg actattcaag cacagatttt ggagctgctg
    19081 aaagaaattc agacaaaaat cgatacatca attatcttta tcactcatga ccttggtgta
    19141 gtagcgaatg tcgctgatcg tgtcgctgtc atgtatggag gaagaatcgt tgaagttgga
    19201 acatcggaag aaattttcta caacccgcaa catccctata cttggggact gcttggttca
    19261 atgccgacat tagatagtgc gaacgatcga ttgtatgcta ttcctggttc tccaccggat
    19321 ttattgaacc cgccaaaagg agacgcattc tatccaagaa atgaattcgc gatgaaaatc
    19381 gatgcagaac aagaaccacc attttttgaa ctatcaaaaa cacacaaggc agcaacttgg
    19441 ctacttgctc cgcaggcacc caaagtgaca ccgccagaag aaatccaacg tcgctgggct
    19501 atctttaaag agaaacaaaa acatacttac ggtcaaggag gaattgctga tgccaaataa
    19561 actacttgaa gttaaaggat tgaaacagta tttcaatgta ggaaaaaaga atgaagtaca
    19621 tgctgtaaat gacatcagct ttcacattta tgaaggagaa acattcggtt tagttggaga
    19681 atctggaagc ggaaaatcca ctactggccg tacgatcatt cgcttgaatg aacctactga
    19741 tggtgagata ttattcgacg gtcaagatat aacgaagatc aaagataaaa aaggtttgac
    19801 gaaattccgg catgatgttc agatgatctt tcaagatcca tacgcttcgt tgaatcctcg
    19861 tatgaaagtc agagatatca ttgccgaagg aattgatgtt aatggactag ctaaatcatc
    19921 tgaagagcgt gcaaaaaaag tcgatgatct attgagaact gttggattga atccaagcca
    19981 cggtactcgt tatcctcatg aattttcagg tgggcaacgc cagcgtattg gtattgcacg
    20041 agcattggca gtaaaaccgc gttttatcat ttgtgatgaa ccaatttctg cactagatgt
    20101 gtcgatccaa gcccaagtgg tcaatttgtt gcaagatctc caaaaagaac atcaattgac
    20161 ctatctattt attgcacatg atttgtcgat ggttaaacat attagtgacc gaatcggtgt
    20221 aatgcataac ggcttgttat tagagatggg aacaagtgat gaaatctata atcacggtgt
    20281 tcatccttat acagaaagtt tgttatctgc tattcctcta ccagatccag accatgagag
    20341 aaaacgcaga agaatcaaat atcaacctga accagatgac ggacaagtac gtcaacttcg
    20401 tgaaatcgca ccagaacatt ttgtctatgc aactgagcag gaagtcccat attacgcaaa
    20461 aaaactaaaa cgacaaaagg aatctcttct ggttgctaac taggagggac atagatgcta
    20521 aaagctttta aattccgtat gtatccgaca gaagaacaaa aacagcaatt gattcgtact
    20581 tttggatgtg ctcgatttac ctataaccat ttgctgaaaa agcggcagaa aagttggcaa
    20641 caaacaggag tagccaattt ttcgctcaca cccgctacat taaaaaaaga atacccattc
    20701 ttaaaagaag tagacagtct tgctttagca aatgcacagc ttaatttgga tcgggctttt
    20761 cgcaattatt tcaagggacg tgccagcttc ccaaagctta aaacgaaaaa aagtatgtgg
    20821 caatcttata cgaccaacaa ccaaacccgt acgatctatc tggaaaatgg ctacttaaag
    20881 ttaccaaaac aaaaagaact aatcaagata aatcagcatc ggccagttga aggatcgatt
    20941 cgctccgcta cgatttctgc tcgatataat gaagaatttt atgtcgcact actttgtgat
    21001 gtttcgccag taaaaaaaga atctttagct aaatggatcg ggatcgccta tcatccgaag
    21061 acattgatcc aaacatctcg acctctagaa gtaactttac caaaatttca ccagacagaa
    21121 gaaaagctgc aacatgcaca gcggaaattg aatgtgaaag tccgctccgc tcaccataga
    21181 aagatcaggt tagatcaggc aagcaattat cagaaacaaa agagaaaagt aatggatctt
    21241 tatctgaaac aaaagaacca gcgagaagat tatcttgaac aattatctgg gaaattggtc
    21301 aaacaatatg actacttatt tgtagaatct tttccaaaag aagaggcaca tgctgatttt
    21361 tctattcacg actggcataa attaataaca aaattgcgtt ataagtccca atggtataat
    21421 aaaaaatttc ttttgatcaa taccgacgga gcagaagaaa gcaattccgt aagaaagagc
    21481 caagtagtag aaaaaatggg cagacattcg gttatcaagg gataaaaaat gaatgaaact
    21541 gataggagcg ttagggttag ccttgttaat aaaaagtctt aatgactttt gtttctagga
    21601 atttttcttt tgaagaaaag ctcactaatt atgacaaacc aataaaacaa tagcagaaaa
    21661 agttcgtacg agctttttct gctattgtta attttttatt cagaaagttt acgaatacat
    21721 caaaattctg aaacaaaaaa cgtaactcac aaagagttgt ttttgcgttt tttcttgaat
    21781 gattaggtct gaatcttttg ctcacaacct tattcatggt gccccggctt gagctgatag
    21841 atttttgaag gacggccgat tcctacagga cgttcaccga tctcatcgaa atattggagc
    21901 atagctttct taaaattgga atgatcgatc gttttataat cgatccctaa gaatttagca
    21961 aatacttttc gagcttccgt aatcgtgaaa tctttaccta aaacttgtag aacttgcggc
    22021 tcgtgttcca ttttattgac tacacggttg aatgctttca agatgatttg actatggtca
    22081 aaagccaaag tatctttgcc agaagactct ccagtgacta aatcaagcga gatagccact
    22141 tctccgctag ataaatggat cttgttacta tgacgttcta gtgtgaacca gcgtacttcc
    22201 ttggcatcat ctccggcgat tagcggttct tctccaataa aggctaagta gctgactgtg
    22261 acgacccagc ctcgcggatc tcgattaggc gtactgaatg tatgcagttg ctcaatattt
    22321 tcttttgaaa taaccacacc agtttcttcc tttgtttctc ttaagacgct ttctccagtt
    22381 gactcatcct tttggacaaa gccaccaggc aatgcccatg aatttcgata aggatgccct
    22441 tttctttgga tcaacaaaac tttcagctga tcttcttctt tgttatagca catcagtacg
    22501 atatcgacag tcaaagaggg cttgtcatat tctggacgtt cctgtttttg ataccagtta
    22561 agaaattctt cttcacttgc tttatgttca taatataatt tttcttcttc tttagaagca
    22621 aaacggttca tctgaccacc tccattgaca gtaaaagtac tttttacctt attgtagcag
    22681 actctgaaaa aaaaccaagg atttacttga aaaatctagg ttatcattta aatatgaaaa
    22741 ataaatggaa gtatgagatt gaataaaaag aaaagcagat aaaacggcaa tactttatac
    22801 cgctcttcac gaatggcaaa gaagccacaa ataacgatat atacgtcaat gataaaacgc
    22861 aaaatccaag ctgctgtcat aaaactgttc aaatgcccat ggaggttaaa ataaccatac
    22921 atacgaatat aaaaatagct gaatattaaa tgtgctaatg aaagaaaagc aatactaagt
    22981 gtaaaaaaaa gatgtttctt ctgcatgatt gttccagtcc cttactaaga tagatagtct
    23041 ttattatacc acgtgtgagg ggagaaataa aaagccgttt tttagtagat atgaaacaaa
    23101 acaaaaaccc gcatttattc aacctattca tccttttaaa ggatagattg aacgtagata
    23161 gaagtcgttt atccagtctt tttcattttg aaaaaaagct aacagcatgc tttttttatg
    23221 gtatactagc aaggaaacga attcgcatca tgcgataaag gaggaagagc cccgtggcag
    23281 aaaaggaaac tttttatatc actacgccaa tctattatcc aagcggcaaa cttcatatcg
    23341 gcaactcgta tactacgatt gcatgtgacg caattgctcg ttataaacgg ttgatgggct
    23401 ttgatgtgtt ttatctcact ggagtggatg aacatggtca aaaaatcgaa acaaaagcag
    23461 aagaactagg tgtaaaaccg caagaatatg tagataaaat ggcggctgat gtcaagaaat
    23521 tatggaaaac gcttgatatc agttatgata aattcattcg cacgacagat gattaccata
    23581 aagcagctgt tcaaaaaata tttgatcgtt tattagaaca gggagatatt tacttaggtg
    23641 aatatgaagg ctggtactct gtttctgacg aagaattctt tacagaaaca caacttgcag
    23701 aagtgtacaa agatgacgaa ggtaacgtga tcggtggtaa agcgccaagt ggacacgaag
    23761 tagaattggt aaaagaagaa tcatacttct tccgcatgag caaatacgca gaccgtctat
    23821 tggaatacta tgaagaacat cccgaattca tccagccaga atctcgtaaa aacgaaatga
    23881 tcaacaactt tatcaaacca ggattagagg atcttgcagt ttcacgaaca acttttactt
    23941 ggggagtacc agtcaagagt gatccgaaac atgtggtgta tgtatggatc gatgcattat
    24001 ccaattatat tactgcacta ggatatggct ccgaagatga aagtctattc gaaaaatact
    24061 ggcctgcaga tgttcagatg gtcggtaaag aaatcgttcg ctttcatacg atctattggc
    24121 caatcatgct gatggccctt gatttgcctc tacctaaaaa agtatttggg catggctggc
    24181 tattgatgaa agacggaaaa atgtctaaat caaaaggaaa tgtcgtttat cctgaaatgc
    24241 ttgtggaacg ttatggttta gatgcactcc gttattattt gcttcgtgct gttccgtttg
    24301 ggtcagatgg tgtctttacg ccagaagatt ttgtttcccg tgtcaattat gatctggcaa
    24361 atgatctagg gaacttattg aatcgaacga ttgctatgat caacaaatat tgtgatggac
    24421 tcgttcctga ctatgcctca cttgtcactc catttgatag tgaactgtct acgacggctg
    24481 ctaacgttgt cggacgttac catgatgcaa tggaaaaaat ggaattcaat acagcattgg
    24541 ctgaaatctg ggtgctgatt tctcgtgcaa acaaatacat tgatgaaaca gaaccatggg
    24601 tattagcaaa agacgaagaa aagaaagctg aattgaatag tgtcatgatc catttagctg
    24661 aatctttgcg tatcgcagcg atattacttc agccaatcat gacagaaacg ccagagaaga
    24721 tcttcaatca actaggattg gactctgaaa caatgaatct tgaaggatta cactttggcg
    24781 aatttccttc aggaacaaaa gtagttgcaa aaggtacacc aatcttccca cgtttagata
    24841 tggaagaaga agttgctttt attcaagaaa aaatgtctga aggtactcaa acaaacgaag
    24901 atacagtcaa atgggatcca gaagaaacag aacttgtttc aacgaaagaa aaacaaatca
    24961 aatttgatgt atttgaaaaa gtagaactaa aagtagcaga agtcatcaac tgtcaaaaag
    25021 tagaaggggc agataagttg ctgcaattcc gactagatgc tggagacagc caagaccgtc
    25081 aaatcttgtc aggaatcgct gaattttatc ctgatccaag cgaattgatc gggaaaaaag
    25141 tcgtcattgt agcaaatctg aaaccacgaa agatgcgtgg acaaatcagt caaggaatga
    25201 tcctttctgc agaagcacca gatggctctt tacaagtaat cgaagcacca aaatccatgc
    25261 caaatggctc agaaatcgca tagaaattca aagggggtat gacactagtt gtgtcatacc
    25321 ccctttttcc gaaatttgag aagcaatttt cgtaaattcc ttcttattac ttgaagccaa
    25381 tcatataata cagaaacaga gaactatcca aaataaatcc tagaaaaaga agtacgagct
    25441 tcgctgattt ttgttaattt agaataatac taataggaat cgtaaaaaca gaaaaaaagc
    25501 ataatgataa agggtcgggc agattttaat tgattcatct ggtactcttt tttattgtcc
    25561 ttacattcat aaagtttgaa tgcaatgact ttattacatc tatatttata tctaacagta
    25621 gcgttgatgt gttaattata agttctatca ctttatgttc aattagtttg tgctcttata
    25681 tgtgtgattg aaaatataga taaaaaaaca atcatcggct ttgacttcga tgattgtttt
    25741 ctttattttg taaaaatcct atcacttgat tttatggtaa ccttttattt ttgtcttcaa
    25801 acggcttggt aagagagcag taagcagtgc aaatgggatc gaaaagacat accagaaaat
    25861 cgtacgtccg ataccataat gttgatagag ctttgtcaac atcacgtgta acaagatcaa
    25921 ccctaaaagc aaggagccag ctataaacca atgattatcc caatgcatga cttcactaag
    25981 tgccatcact ccaaaaggat agaaataaaa atagatcccg actaaggtca tccgccagtt
    26041 ctttgtaaat gttccgatat tcaataaaaa gaaaatcgct gaaagcaaaa taccgatagg
    26101 cggaaaaagc ccaaccaaaa aggaatacaa catcccccac aagatcatca caggaccttt
    26161 gatcaatgca acgattgcga tgaacaaagt cagcaatatt agtttttgtg ataacgttgc
    26221 aacagtcatt aatccaaata aaattaatgt gaaccaatgc caatgggact gctgttgttc
    26281 tttacgggaa aaatcgaccc ggttggataa ataatctgct aatttcggat aagaacgctt
    26341 aaaatccata aactgttcct ccttcgtgga aaaggtaggt ttagtatacg aaaaataaaa
    26401 aagaaataca tccgtcttaa gagggagttt ttcactgaaa gtggagaaaa aagaatggta
    26461 aattaaaata ttctcagtta tactgaaaat tataccaaat ttcaaagaag aaagaggatg
    26521 ggaatgaaag gcaaactaaa acgagaaatc aatctgtttg gcgcattagc taccgttatg
    26581 ggaaccgtga tcggtgcagg cgtcttcttc aaaactgctg ctgttactgc tagtactcag
    26641 tctacaagtt tgacattgct agcttggctt ttgggtggtt tcttaacgat ctgtgctgga
    26701 ttgacagtgg ctgaattagc tacagcaatc ccggaaactg gtggagctgt aaaatatatt
    26761 gaagctgcgt atggaaaatt gcccagcttt ttattaggat gggcgcaaag cttgatttac
    26821 tttcctgcta atattgctgc attatcgatt atctttgcga cacaattgac gaacttatta
    26881 caattatcta ccgattatct tttgttgatt gcagtgatca ccgctgtttc tgtgacaggg
    26941 ttgaatttgt tagggacaaa agtcggtgct tctgtacaat cagtgacgtt gattgttaaa
    27001 ttaataccaa ttgctgtgat tgtcatatgg ggattattga ccccaggaca aggaactgtc
    27061 cagttatttc caatcgaagc tggaaaagat gttacttttg tcgaaggatt gagcagtgcg
    27121 ttgttagcaa cgttgtttgc ttatgacggc tggttaggtg tcggtgcaat ggcaggggaa
    27181 atgaaacggc cggaaaaaga tttgcctaaa gcaattattc ttggtttgag ttttgtaaca
    27241 gtcgtttatc tattgatcaa ttttgttttt ttgaaaactt tgccgattga tcatcttgcc
    27301 ggtaatttga atgcagcttc tgaagcatca gacgtcattt ttggaggaat cggtggaaag
    27361 ctggtcacta ttgggatctt gatctcggta tacggtgcat tgaacggtta tacactaact
    27421 gggatccgcg tgccttatgc gatggcttta gaagacgagc tacctttcag taaacagctg
    27481 acaaagcttt caaagaagtt tacggttcct tatgttcctg cggttttcca gttagcagta
    27541 gcttgtatta tgatgagcct tggttctttc gactttttaa cagacatgtt gatttttgtg
    27601 atgtggctat tcagtttact gatttttatc ggtgtcttta ttttaagaaa aaaagcgccg
    27661 gaattgccac gtccatataa agtaccactt tatccaatcg ttccgattat tgcgattctc
    27721 ggtgctattt tcattttagg tatgacgatg atgacgcaaa cgaaattagc attgatcggt
    27781 ataggtgtta ccttgatagg tatcccagtc tattatcaaa aaaagaaaaa acgctcagaa
    27841 gagtaaaata tttcaacaac agacagaaaa agtacgaact aggtcctcta gatcttcgtg
    27901 ctttttctat ttcaataaaa caagttcttc tattttgatt tttgctactt ctgcaagtag
    27961 tgcttatgtg ctaaagtaag aatgaaaagg agagataaac atgatatttg attctcatac
    28021 ccatttaaat gctgaacagt tcaatgaaga tattccagaa acgattcagc gagcacaaga
    28081 aatgggggta acgaaaatgg cggtcgtggg cttcgatacg ccaacgattg aaaagtcttt
    28141 gcaattaagc catgactatc caaatatata cagtattatc ggctggcatc cgacagaggc
    28201 tggcagttat acgaaagaaa tcgaaaataa attacaagaa caattgactt taccaaaggt
    28261 tgtagcatta ggggaaattg gcttggatta tcattggatg gaagacccga aagaagtcca
    28321 agataaagta tttcgcagac agattgctat cgcaaaagaa atgaacctac caatcagtat
    28381 tcatactaga gaagcacttg aagacacgta tcaaatccta aaagaagaag atgttcgaga
    28441 tatcggtgga atcatgcata gtttcagtgg tgattatgaa tgggcaaaac gatttcttga
    28501 cttagggatg catatttcat ttagcggtgt cgtcactttc aaaaaagcat tagacgtcca
    28561 agaggctgca gcaaatgtac caatcgaacg attacttgtg gaaacagatg ccccttatct
    28621 ggcaccagtt ccttttagag gaaagcgaaa tgaaccaggc tatactcgtt atacagtaga
    28681 aaagattgct gaacttcgga atcttccgac agaagaagtg gctgcccaaa catggacaaa
    28741 tgcgcaccga ttatttggtc taagcgaaca tggataagat gaagatccaa gagattatag
    28801 tcgttgaagg aaaagacgat acaagaaggc tgcgggaagt agttgatgca gatacgatag
    28861 aaacaatagg gtctgcaatc aatgaagaga tcctgatgca aattgaacat gcccaagaga
    28921 ctcgtggagt gatcgttttt accgatcctg atttttcagg agaaaaaata agaaaaatca
    28981 ttatggaagc tgttcctcag gcaaaacatg cttttttgcc gcgcacacaa gctaaaccaa
    29041 agaaaaaggg aagccttggt gtcgagcatg caagtgatgc tgctatttta gaagcattga
    29101 aaaaggtcgt gacgccagac atttcccaaa cggctgtttc tgaaatcact agacaagacc
    29161 ttttagacta tggtttaata gctggggcac aagcaaaaga aaaacgagaa aaactaggtg
    29221 acgagttgcg catcggttac acgaacggca aacaattgga aaaacgtttg aagatgtttc
    29281 gtattacgcc gaaagaactc gcccaagcaa tgaaaatagt ggaggaacaa catgagtgaa
    29341 tatcgtgaaa ttgcgacacc ctctagaaca aaagagattt tattgaaaca tggtttttca
    29401 tttaaaaaaa gtttaggaca aaattttctg actgaaccaa atattttaag gaaaatcgta
    29461 gaaactgcgc aaatagatga acatacgaac gtgatcgaag ttggaccagg aatcggtgca
    29521 ttgacggaac aattagccaa gcatgcaaaa caagtcgtag catttgagat cgatgatcgt
    29581 ttgatccctg tattggctga tacgatgcaa ccctatgaca atgtgactat tgtacatcaa
    29641 gatattttaa agaccgattt gtcaacagct gttagagaaa catttcatga ggagcttccg
    29701 ttaaaagtcg tagcgaattt accttactat attacgacac cgatcatgat gcattttctt
    29761 gaatcagatt tggttgtgga tgaactagtc gtcatgatgc aaaaagaagt agctgatcgt
    29821 atctctgctg aaccaggaac gaaagcttat ggttcattat cgattgctgt tcaatattat
    29881 atggaagcca gtttagcatt catcgtacca aaaacagtat ttgtccctca gccaaatgta
    29941 gattcagcga ttttgaaact gacaagaaga gatataccgg cagtcgaagt gacagacgaa
    30001 aaagcatttt tccgattgac aaaagcggca tttcagcaaa gaagaaaaac gctttggaat
    30061 aacttgcagc attcgtatgg caaagatgac cagacaaaag catggttagc aaaaagcttg
    30121 gaaacagctg gaatcgatcc aaaacgtcgt ggggagactt tgtcattaca agaattcgcc
    30181 gcattaagca atgcgatgtc agaaaatatg cagtaaaaaa agaggttgtg acagaagttg
    30241 tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt tcctcatgtt
    30301 tttcgtttgt ttgggcttgt taccgcagat gcaagctttt gaacaccgtt taagaaagag
    30361 gttgtgggcc tgccgtttaa ctctaagtca caaggaacaa tttcaaaaaa cagcttctca
    30421 tgtttttgag ttagttggtc ttattacttg atgcaagctt ttgaccacta ttatgccgaa
    30481 caaggagatg tgacacaaat tttgtcacat ctcctatttt tgtttcttga tcaaaaaatt
    30541 cttatacttg atactcacac tcattttgta tggatttaga aaagaatatg tgtcttgaca
    30601 gttattagta agtgagttat actaagtcga catttatgtg tcaagacata ttaggagaga
    30661 gaaaaaatga gaaaaaattc agttcgaaaa ctaacgattt ctgctttatt gattgggatg
    30721 ggggtcatta tcccaatggt catgcctaaa atcatgattc caccagcttc ctttactttg
    30781 gcgtctcatg tgccattatt tattgcgatg ttttttacac caggagttgc agtggctgtt
    30841 gcgctaggta cgacatttgg tttcttttta acaacgccgg ttatcatcgc attgcgagca
    30901 ttgtcacatc ttgtgtttgc gttgattggg gcttggtact tgcaaaagca tccgagtatc
    30961 gttttaaaaa atggacagtt tacttttgct aattggcgtt tccaagggtt caattttgtg
    31021 atcggtgtga ttcattcttt agcagaaatg ctagtagtca gcatctttta ctttattggt
    31081 aatatgcctg aaacatatta ttctcaagga tatttttata cagtattcat cttgatgggg
    31141 attggtgggc ttatccacag tttagttgat tataatattg cttattttat agcaggaaca
    31201 ctcagcaaat attttgatat ccccgtattt acggcagcaa aaaaagaaaa tcggaaagag
    31261 tcgattcaag ggtacgcgaa gaaaacgaca tattagacat gatctctcaa aaaaaaaact
    31321 ctcatctagt aatggataat aaatcctcta agatgagagt tttttgtcct aaaaagagct
    31381 gcaccaacat cttctggcac agctctaaca gggggcaatt tatggaagtt tgatgacttg
    31441 ttctgtttct ttatcaaaga agtggccttt gctgatgttg aaggcaaggt cgactatttc
    31501 gcctggttta tggaaatctc ttgcatctac ttttgagatg aactcagtag aaccagtttt
    31561 tgtgtaaagc atcgtttcag ctccaagtaa ttcggaaacg acaacttctg atttcactac
    31621 ggcttcaggt gaagcttcaa gggctacttg ttcactgtgg atgtcttctg gacgaatacc
    31681 gaagataact tcttttcctt cataaccttt ttcaactaat actttgtttt ttccttcagg
    31741 aatacgaagc ttaagcccat gtccatctgt gataacacca tttttcaatg tcacattaaa
    31801 gaagttcatg gcaggagatc caataaagcc ggcaacgaac atattgacgg gtgtatcata
    31861 aacttctttc ggtgtaccga tttgttggat aaatccgtct ttcatgataa cgattcggtc
    31921 agccatagtc attgcttctg tctgatcgtg agtaacatag atggtcgttg tttcaagacg
    31981 ctggtgaagt ttggcaatct cagcacgcat ggctacacgt aatttagcat ctaaatttga
    32041 taatggctca tccattaaga agacttttgc atcccgaacg attgcccgtc ccaatgccac
    32101 acgttggcgt tgtccaccag ataaagcagc tggttttcgt ttcaagtatt ctgtcagacc
    32161 taagatctct gctgcattgt ccacacgttt tttgatgtct gctttatcat atttacgtaa
    32221 tttcaagcca aaagccatat tatcaaaaac agtcatatga ggatacaatg cgtagttttg
    32281 gaaaaccatg gcaatatcac ggtctttagg tgccacgtca ttcatgactt ttccgccgat
    32341 atttagttct ccttcagaga tatcttccag tcccgcgatc attcgcaaag tagttgactt
    32401 tccacaaccg gaaggaccaa caaaaacgat aaattcacgg tctttgattg atagattaaa
    32461 atcagttaca gaataatttt ctgcattgtc atatttttta tatacgtttt tcagagccat
    32521 ttctaccata atacgttcca cactttcttt tttagtttta ttaactattt aaagtataaa
    32581 tgaaagcgtt aactaacttc aatgtcatgt tgaacaagaa ttttttcttt tttttgtcaa
    32641 tgtgctagtt ttaagcttta ataaatatcc ataagaaata aaaactgata cactggagta
    32701 aataagtatg ttaacaatat caaaaggagt gtctcttatg aaaatcgctt tgatcgctca
    32761 tgaccgaaaa aaacctatga tggtgaaatt agcaacagcg tataaaacaa tattggaaaa
    32821 acatgaactt tttgctaccg ggacgactgg gcagaggatc acggaagcaa ccggcttgcc
    32881 gatccatcgg tttaaatccg gaccgttagg cggggatcag caaattggag ccatgatttc
    32941 ggaggatcag ttagatttgg tgatttttct tcgagacccg ctggctgcac agccccatga
    33001 accagatgta acagcactta tccgtttatc tgatgtctat gagattccgt tagctacgaa
    33061 tatcggaaca gctgaggtgc tactaagagg actggaagcc ggcttcctag attttcgaag
    33121 aatcgtccat gaaatagaca agcatccact ttcattttga tggataagcg tatggaaaaa
    33181 taacgtttaa tgttcgccta tatgtagcta taaaagctaa acaaactgat aatatgcgat
    33241 ataatacgaa cgtattggat aatataaaca ataatattag tttttcgtat ttgcagtaaa
    33301 tgtgaaaata tgctatagtt tctgtgggaa aagatcccaa aaataataca gagtagaaaa
    33361 taaagcgtta agtgctaaga ggatgggatg ttgcctcttg gacgaaggac gtaatgttcg
    33421 cggttttatt ttcgcattcg ctgcatgata cgatgtagca gctcttaaag gagatgctaa
    33481 aaatgaagaa acaccggtta gacgcacgta tgattgctat tatgggctta ttgattgcat
    33541 tgatggtcac tttgtcccga ctggtagcaa tcgagacccc atttattaaa atcagtgtca
    33601 catttattcc acaagtgatc atgggcattc tttttggtcc tttttggtca gggataggcg
    33661 cagttttagc tgatttagta ggaatggccc tcttctcgaa atctgcattc tttattggat
    33721 ttacattgaa tgcatttatt gaaggagcaa tctacggatt ctttttctat cgaaaagaga
    33781 tcacttggaa aaatgccatt ctggctacgc ttagtgtcac gctgatcatc aacttatttt
    33841 tgacaccatt atggcttgcg ttgatgtacc atgtgccatt gttcagctgg gtcgtttggg
    33901 cgccccgcct gttaaaaaca gtaatatggt taccgattca atcaattgcc atttattacg
    33961 taggacgtag tattccttac aaaaaaattt taagaagttt agcgatccac gctaaataaa
    34021 aaacaagcag tcttcccatc ccagaagact gcttgttttt attgtgctgt gaattgtgct
    34081 tgatagagcc gtcggtaata accatctttt tgaagcagct catcatgggt gcccatttcg
    34141 acaatagctc cctgatccat cactaatatc tggtccgcat ttttgattgt ggacaggcga
    34201 tgggcaatca caaaactggt ttttccttcc atcattgcta agaaggcttg ttggattttt
    34261 ttctctgtca atgtatcgac tgagctggta gcttcatcta agatcaacat agggggatta
    34321 ctgatcatcg ttcgagcaat cgtcaacagt tggcgttgtc catctgatat ctttaaccct
    34381 tgaccgccaa tcttagtatc taatttttcc ggcaaacgtt ctacaaattc atacatataa
    34441 gaggctttta gggcatcatt gatttctgcg tcacttgctt ccggattccc aaaacgaaga
    34501 ttttctcgca atgtactgtc aaatagccaa gtatcttgta acaccattcc aaaactttta
    34561 cgtaatgtat cacgttggat agagcggatg tcatggtcat cgactgtaat tgttcctttt
    34621 gttgtgtcat aaaaacgcat cagcaaatta acaagtgtgg attttccggc ccctgttttt
    34681 cctactatag caatggtttg acctggataa gcagtaaaat tgaaatcttc gatcaaacgc
    34741 tgcttggggt cataagaaaa agaaacatga tcaaaagaga cttttccttt gatatttttg
    34801 ggttccaagg cataggcatc tggcaaatca ggcttttctt ctggctgatc gatcaaatca
    34861 aatgctcgtt ctagaccaga aaaagcggtt tgcagttgtg tcgtgattcc agaaagttcg
    34921 ataaatggtt tagaaaactg acttgagtag atcgtaaagc tggaaatcac accaacagtg
    34981 attgacgaac cactcttcaa tgccaatagg ccgccaacta aaccaatcga taaataagca
    35041 aggtgatcta caaaacgaga agacgggttt gtcaaggaag aagaaaattg tgcccgttgt
    35101 cctttttcat atagatcagc atttagcagt tcaaattgct gttgactagc ttcttcttgc
    35161 tgaaaagctt tcacgatttt ctggttaccg attcgttcgg tcacaaagcc cgagatatcc
    35221 ccaataatcc tttgctgtct atcgaaattt gtttgtgaag cacgagcaac gatccaactc
    35281 acaaggaaaa taattggtgt agaacaaaga acgaccaatg tcagaagtgg acttagacgc
    35341 atcataaaaa taaacgaaag aatgatgatt gccactccag aaaataattg attgaaggcc
    35401 gcagaaattg caatggaaat attgtccata tcatttgtaa atcgactgac gatatttccg
    35461 tgggagtttt gatcataata acgaagtggc aatcgattca gatgagcaaa tgcatctttt
    35521 cttaattttg caacggataa gtaagctaac cgattgctta acacttggat gagccattga
    35581 ctgactacgt tgattaaaac gataccaccg aatagaataa gtaaatggaa cagtcgattg
    35641 aagcggacct gtccttctcc aatcatttga tcgatacttt gaccaatttg taacgtcatc
    35701 acaactgttg caatcccatt gatgattcct agtatcacag ctagccaaat gtcttttgca
    35761 taggcagtaa ggtaaggaat gaatcggcgg agcgtctgca aggataattt ttttactttc
    35821 ataactattc ttcctcctgt gatgcgacga tttcctgata ttctgctgat tgagcaagta
    35881 attcttcatg ggtaccctga gcagcaatct taccttgatc catcactaag atatgatgtg
    35941 cttcttgaat agagcggaca cgttgggaga taataatcac tgtactgcca agataagtct
    36001 gtagcgcctt acgcaaatcc aagtctgttt gataatccaa cgcacttaaa gagtcatcta
    36061 aaatcagtaa tgctggcttg gcaatcaaag ctcttgcaat cgtcagtcgc tgtttttgtc
    36121 cgccagagaa atttttccca ttttccatga cttttgtatc taaaccattt ggaagctgac
    36181 ggacaaattc ttctgcctga gcgattttca gagcttccca acattctttt tctgtggcgt
    36241 ctttcttacc ccattgaagg ttttcacgaa tagtcccggt aaatagtaca gaagtttggg
    36301 gaacaagagc aatttgctga cggagatgat ccagctgcca agaacgaaca tccattccgt
    36361 ccattataac tgtaccggaa ctgacatcat agaaacgcgg aatcaattgt gtcaaagtcg
    36421 ttttcccgct tccggttgga ccaatgattc caaggactgt atttcgcttc agctgaaatg
    36481 taatattttg gagagaaaga ccagaatcag gagtataacg gaagtcaaca tggtcaaaag
    36541 cgataattgc ttcagaagaa aaatcgggag tagtactgac ctcagcttca agaatcgaat
    36601 tttctgtgtc tagcacctct ttcacccgat ttcctgaagc ttcggcacgt gtgaagatca
    36661 cgacgaggtt tgaaacaacg atcagtgcca aaagcatttg attcatataa ttgatcaaag
    36721 caagaacttg tccttgttgc aagctaccaa tatttacttt gatcccgcca actgtcagca
    36781 aacagatgat cccgacattc atgatcaagg ttgtagcagg tgataatagg gcagagatat
    36841 tggaaacacg taaataattg ttcgctagat catttgtgct tttatcgaac ttctctgttt
    36901 ctttttttgt ctgcgcaaat gcgcggatga cacgaacacc gctaagattc tgactaacgt
    36961 gttcattgag ctgatctagt ttttcttgca ctttttggta gagaggaacg gtttttttga
    37021 tgataaaaaa taaaatgatt gagaaaatag gcaataatgc taagaaaaac agacctactt
    37081 cccagtcgat cacaaaagcc atgaccactg acccgatact taaaaacggt gctctgatca
    37141 ataaacgaat gaccattgct aaagctagct gaagctgatt gatgtcgttt gtcattcggg
    37201 tgatcaaggt atctgttcca aaatgattca actcgttgtg agagaaagta ttgatttttt
    37261 tgattagctg attacgtaac tctgtaccaa atccttgtga agctactgaa gcatagtatt
    37321 ggcaaatgat agcacaaatc agaccaatca cagacatagc gaccatccat aagcccattt
    37381 caatgatttt tcctcggtca ttcattttta gtccattatc gatcaaggaa gccatcatta
    37441 aaggcaggac aagttcgaag acagcttcaa gaaatttgaa gaacgggccc aaaatgattt
    37501 gcttacgata gtcttttgca taacgtaata actgaatcat atgattcctc ctattattta
    37561 tttgtccaca attgaaaaaa caagtactac cacattgtag cttaggaaat ttagaatgaa
    37621 aagagaacaa gccttttcat aaatgaaagt tttattatga agaagcttta gaaattgaat
    37681 agactatttt ttttaaaaaa aagaaaatga gaaaacatgc cagtctcttc attcatagga
    37741 acgtctattt tacttaattt ttctataaat gaattctgca taaaaaatat atcaagtaaa
    37801 tgtttataga tgaaactcta tttacttctt ttttaatttg ttcttgcaat cttttttgct
    37861 acgtaatttt tgtaaaaaag tgggttgaga gaaatctaat tgattttcaa agaaggagta
    37921 tgaaaatgag aaaaatattt attcgtttat tgtgggtact atcttagagg aataaataat
    37981 agtggagaaa actatggttt ggcgcagatt ctatcaggtt tgatggaacg aagacaacct
    38041 cccgacatca agtcaaaagc tacttgagat tttgatctga aaataaaaaa agaaaacaag
    38101 gtaggaacat caatgtttct accttgtttt cgtaatcaat gggaaaaaac taagagtggt
    38161 gtacgtaatc aataaaaagg atggtgacaa cagtacctct ttactctctg aaagcatcat
    38221 aactcgtttt tcctcattat gccaataaat tgttgttata ccacatataa ggatttgtaa
    38281 tagctaaaaa cagacaggaa agttgaaatc tgtgaatctt attttgtatg gaaggattta
    38341 tagaacgcaa aaatcaagat aaagatgaat aaaaatcctg tttcggctat atattgcgta
    38401 tctacgccaa taaaacgagt ttgttctgtc atagccatat aaagcaaagg caaaaataca
    38461 actacagcga gataagatcc gatgatttct cgaaaagttt tccaggaagt atgtttcctg
    38521 tttttatatt tatccgattt cattgaatag tcatagcctc cttattcttt catatgttga
    38581 aattccagta taaaaactgg aaggaactgt acgtttttta gaataaaaat tataagggat
    38641 aagtaggctg ctgtctataa taagatatat ttttcaattc tttattaaag attttattga
    38701 caaaatgaat ccgttttcta tgatggagga ggggtgaata agttgttgaa gaaggaaatt
    38761 ttatgttact tagagaacca gacagccttt ttcgatttag aacatatgaa cgaggtcttt
    38821 acagcacaaa ggttagctga tcattttggg gtaaaaagaa acacagtcag tcattatttg
    38881 aaccagttaa atgatgagca actactagtc aaaatcaatt ctcgaccagt gatttatttc
    38941 cacaaagaag cttttgaaaa acaatttttt aagttacgca cgaattttta tttttctatc
    39001 gatgatctca aagaggaaca gccttttttc gcgcaaccaa aagacttatt ctcttttatg
    39061 atcggatacg acgccagttt gaaagaaagt atcgaacaaa tcaaaacagc tctttactat
    39121 ccagatggcg gattaccact tttgattaca ggcgaaagtg gcacaggtaa aagttattta
    39181 gttaatttgg tctatcagta ttgtctgctt catgatctgc tagaagattc tgcaccattc
    39241 atcactttta attgtgcaca atatgcagat aatcctgaac ttttgactag caatttgttt
    39301 ggacatgtta agggagcgta cactggtgca gaagaaaata aaaaaggggc ttttgaagca
    39361 gcagatggtg ggatcttgtt tttagacgag gtacatcgct taacaccaga aggtcaggaa
    39421 aaactgttca cctatcttga tcaaggtgtg atttatcgta tgggagaacc taatttgtca
    39481 cgccggatca aaacacgtct cttttttgct acgactgaag agattactag tcatttctta
    39541 acaactttca ttcgaagaat acctatccga atcgaactac ctgcacttaa tcagcgtagt
    39601 cgtaatgaac ggctagaact gatttactct ttttttctcg aagaacaaag aaaaatgggg
    39661 agccctttgt cagtcagtgg accagctttg tcacttttaa caagtcagaa attatccgga
    39721 aacataggag agctgaaaaa tatcgtcaaa gtttcagcag caaaagcata tgctgaacag
    39781 cgtgaacagc ctgaaatcca tgtcacgatc catcatttgc aaaaagaact acttgcgcag
    39841 cctattacta aaaatacagc acagcaagga atctatatta cgcaagacca gaccttagag
    39901 caattaatgg agaagcaaca gcccgaacag cagcgaatcg ttaaaagttt tgagcaaatc
    39961 ctaattgatt tcaagaaaaa taactgtctg ctatattctt gcgaaggaaa attaaagcaa
    40021 gaagtgattc aattatttga ttttttgctg tttgagacca gtagacaaga aaaacacgag
    40081 ctgctggttt attggacaca atctatccga gagacctttc gacaaatgga aactgcctat
    40141 caaatcaaat tcgatggaaa cagtgtctat gccttgagtt actatctcta tcagcgaagc
    40201 actatcaaat ggttaccgga agaacatgat attatccaac taatcaaaga attagaacaa
    40261 caagtttctc agtcttatcc atctagttat cactatgtcc aacgaattct ggaattaagt
    40321 aagatcaaat tagatattga agacacaagt atggatcgga ttttactgac gatctatcta
    40381 aaaaaagcga aatggtcaaa agaaaaacga ctgccaaaag cgatgattgc tgctcacggt
    40441 tatgcaactg ctagcagtat ggcaaatgta gtaaatcgtt tattacaaga ggatctattt
    40501 gaatcatttg atatgccact agatgtcaca ccacagcaga ttgcttcaga aatccttgat
    40561 tattgcgaaa acagcgatgt ttcaaatgga ttgatcatat tagtagacat gggatctctg
    40621 aaagaaatcc atcagttttt taaaaaacag ttatccgtac cgcttttgat tttgaataat
    40681 gtgacgacac ctttagcaat cacagtaggg gaatgcctgc aaaaaaatgt atcattagaa
    40741 gagatagcag aagagactgt tcgacagatc cagccagaat ggcgactgct ctatccagag
    40801 gaaaacaaac ccaaagcact cattactact tgcttcactg gaatcggtac ggcggtccac
    40861 ttaagtgaac tgctagaaaa aagcttacct acggcttgtc agttgaaaat catcccatat
    40921 gaatatcagc aattaaaaga taagaaaaac agtgacccat tattttccat atacgaagtc
    40981 gtaggaatga ttggcaccac agatcctgat atcaccaata tcgactatct ttcattagaa
    41041 gacctcatct cgggggaaaa gatgtctgta ctagctgaat ggttggacac tactatgaat
    41101 gcttcagaga aggaaatatt caatcagcgc atcattcgta atttttcatt ggaaaaagta
    41161 ctggattccg tgactattct agatacagag aaggtgatgg gagaaatcga tcattttatg
    41221 agagaattag aggtgcagtt gaatcgaagc atcagcaatc ccaaaaaatt agcgttgtat
    41281 gtccatgtga gctgtctcat tgaacgattg atccgacaaa tgccaatcga aacctatcaa
    41341 ggattagaga aatggaagca gtgtcaaaaa gaagggttat ctgctattaa atctgcgttt
    41401 agtgtcattg aagagcatta tagtgtcatg atccccaatt ctgagattgc ctatatctat
    41461 gatattttga gtgaagatac agagtttctc tcgatagaag aagaattttg aacataagca
    41521 agttgttgcc actaattttg gcatgcaact tgctttttta gtatgtgtaa gtgaaaagag
    41581 gagatgaaaa agtggataga gaaattatct tagcttccca tggaaaattt gcttcaggga
    41641 tattagattc attagagctg atctatggga aaaatcattc tattactgta ttcgattgct
    41701 atactgatga gaattttgat ttagcggaaa cagtaaggca gctttttatg aggtacaaag
    41761 ataaagaaat cattgttctg acggatcttt ttggcggaag tgtcaacaac gaattcttac
    41821 aatatatcaa tcgggatcat gtctatctcg ttgccggatt gaatttgcca ctattgattg
    41881 agctagtatc tcggttggac gctgaagtag agacaactgt tttgatccag caggtgctaa
    41941 ctgattcaaa aaaaatgatc cagttttgta acgagcggct caatcagaaa atagaagaag
    42001 acgaatttta ggaggaagaa aaatgattgt attgacaaga gtcgaccatc ggttattaca
    42061 cggacaagta gctttttcat ggacacaaac aataggcgca gactgtattt tgattgctaa
    42121 tgatgatgta cctacgaatg agatacgaaa gacaacgatc aaattagcca agccacaagg
    42181 agtcaaacta gtgatcaaat caattgatga ctcgattgcc gcgttgaaaa gcggggtgac
    42241 agataaatat aagttattca tcgtagtgga atcgatcgag gatgcatata agctggcaga
    42301 agcgtatcca gaaatcaaaa ccatcaactt aggagggatc aaggcaaaac ctggtactcg
    42361 cagcatcgga aaagcggtaa atattttacc agaagaggag gaacgcttga aacgattaat
    42421 tgacaaacaa atagaagtag aaatccgtca agtaccagca gataaaaaag tggatgtaag
    42481 agaagttttg taagaaagga agaacctcaa tgattgtaca agcagtctta ttaggaattg
    42541 ttgcatttgt cgcacaatca gaatatgcac tagggacatc gttgctttct cgtccgattg
    42601 tgacggggct gtttacagga atcgtgttag gtgatgtaaa aactgggatc attatgggcg
    42661 caactttaga gttagcattt atcggatcgt tctcagtagg tgcttcgatt ccacctgatg
    42721 ttgtgaccgg tgggatttta gggacggctt ttgctattac agctggagcc ggtacagaaa
    42781 ccgcgctttt actaggtttg ccgattgcta cgttgacatt gatattgaaa aatatttact
    42841 tagggctttt gattccaatt atgaaccata aagcagacaa ttatgcagaa gaaggaaatt
    42901 ataaaggagt cgagcatatg cacttgcttg ccggtatcgg tctttccttg atgcttggtg
    42961 ttgtggtaat ggtttcttat atggtgggaa gcaatacgat cggaaatcta ttaaatatga
    43021 ttcctgactt cgttcaaaag gggttgtcag tagctacagg gttgattcca gctcttggtt
    43081 ttgcgatgct tgctcgtttg ctgatcaaca aacaagttgc accatatttt ttcttaggtt
    43141 ttgctatagc ttcttactta gagattcctg tgaccggagt agctattttt ggtgcgattt
    43201 tagcagttgt ggtagtcaat attatgaatg tccgtcaact accacttcaa acaactgcag
    43261 gggaggtaga agaagatgaa gaattctgag atcaaaaaca gcaaaataac gaaaaaagag
    43321 ctaaatcacg tattttggcg ttcttttcaa atggagtttt cttggaatta tgaacgtcag
    43381 atgaatatgg cttatgttta tgcaatgatt ccgattttga aaaagttata tcagtcgaaa
    43441 gaagaaatgg cagcagcttt gaaacggcat ctagagtttt ttaacacgac cccacatatc
    43501 gttacactga ttttaggaat cacggctgca atggaggaag aaaataagga tgatcctgaa
    43561 tttgatgtga ctgcgatcga tagtatcaaa acatctttga tgggacctct tgcagggtta
    43621 ggagattctt tcttttgggg aactttgcga ttgattgcca caggtgtggg tacctcttta
    43681 gccatgcaag gaaatatttt aggccctatc ttgttcattt tgattttcaa tattccgcac
    43741 attctgtttc gctatctagc aacaggttgg ggatatcgct taggaacggg ttttttgaaa
    43801 aagatccagg caaacggtat gatggaaagt ttgacattag gcgcgtctat tattggactg
    43861 atggtcgttg gagcaatgac tgccacgatg atcaccatca atattccttt aaaaatcggt
    43921 tcaggagaaa atgctgtgac tgttcaaagt atttttgatg atatcgttcc aaatatctta
    43981 tgtcttggag catttggcat agtgttttat ctattgaaga aagaagtgaa accattaacg
    44041 atcttgatcg gactagcttt ctttggaatt tttggttcgt ggatagggat tttttaaaaa
    44101 aggagcagag aaaatgttta cgatgcaaga ttatatctat gaagaaaagg aagtactaag
    44161 tacgatcttg aaaaagaatg atttttcgac aagagaacat atgaaaaaaa cagtgaattt
    44221 actgatctta gcaacgggtt catcttataa cgcttgttta gcagcgaagc cagcattgga
    44281 atcttatggt gatcttactg tcgatattca agaacctttt tattttttca attatggaaa
    44341 actttctcct tcaatcgata ctgtgattgc tgtgtcacaa agcgggaaaa gtgcttctac
    44401 tgttgaagca gtaaaaatga tacaaaaaca agggttacct gttgttgcta tcacaaatga
    44461 cgtgcaaagt ccacttgctt tagaagctgc acaaattatt gatttaggtg ccggggtaga
    44521 atcagtaggc ttcgttacaa aaggctattc tgcgactgtt ttacagttac tactcttagg
    44581 gataggaatt ggaataagta aaaataaaat cagcaaaaaa atagaacaag actacatgca
    44641 acagttgcgc aaaataatca accatttacc agcgattatt caaaaaacgg aagaattttt
    44701 tgatggatat caaagtttat ttcgtttagc acagcgtttc gtcaccattg gatatggtcc
    44761 aaactgggga acagctaaag aagcagagac gaaattgact gaaacaatcc gcgttccgtc
    44821 acaaggattt gagttagaag cttatatgca cggcccatac ctggaggcag atgcttcgca
    44881 tttgctattc tttattgaag gggaaagtgt aaataaggaa cgttcgcaaa agttgcaaag
    44941 atatatgagt cggtacgttg gagagactct caccattacc acaaaaaaag caagaaacga
    45001 gaaaacactt ggattagcaa tagaatgtga tgaatatctg tctgtattag cgcttgtcgt
    45061 tccgtttcaa ctattcgctt ataaaaccgc agtagctaaa ggtattgact tgaacaaaaa
    45121 gatttttgaa gactttgata cagtcttgaa aagtaaatta taagaggtgg aaatcatgct
    45181 gaaattcaat gaagaagaac aaatcaaagc aataaaaggc gcactagctt tacgtccgca
    45241 agtagaagaa atcatcgata aactttatgc ggaaaaattt gacgctgtct attatctagg
    45301 aatcggtgga acgtatgctt cctcgatgca agctgtcact tatatgaatg ggaaaagcaa
    45361 cttgcctgtc tttgtacagc atgctgcaga atattacact actggaaaca aaagattaac
    45421 aaaagattct atcgttattt tgtcctctgt taccggtaca actcaagaag tagtgaaagc
    45481 agtagaacaa atcaagaaag taggagcgac actgattgga tttatcgata aagcgaacag
    45541 taagttgtct cagctatgcg attttgtggt cacttatcca gctcctggaa cagaacaaat
    45601 caaatttttt atggcagcag atcgattgat gtatttgaat ggagaattcg aagcttattc
    45661 agaatactat gaacagctcg aacgttattt accaacaggg ttagtggaag cagagaaaaa
    45721 agcagatgca tttggcttat catttgcaga aaaacatcga cgtgatagta tgcattattt
    45781 tattggagca gggaatcaat ggggagctgt ttattcatat gccatgtgtt actgggaaga
    45841 acaaagctgg cttccatcga aatctatcca tgctgccgag tttcttcacg ggacgttaga
    45901 gatcgtagaa gaaacaaccc cagtcacatt atttttaggc gaagatgaac agagagtgtt
    45961 atctgaacgt gtggctaaac ttttgccaaa aatttgcagc aattatacat tgatagacac
    46021 aaaagactat ccagtagaag gtatcagtga aaaatatcgc ggacgtgttt tgtctttctt
    46081 attgatgcat gtagtgacac aacgcatcga tgctcatgtg gagcagttga actgccatcc
    46141 attagagata cgccgctact atcgtcagtt tgattattag gatagactgg ataaaaagaa
    46201 tataaaaaat aaagagagca gcgataactg ctccctttat ttttttaaaa tattggcatc
    46261 aattacgtta taaaacacat tttttgcatt atctacagtt caacttataa tctgtttaat
    46321 gggacatgtg ggtgccaacc attttttgtt atgcaatagt actatttagt tgttgcatgg
    46381 atccgtagta aaagtccaag tacaacgagt tgcgccagtt tacatagcgt tttgtatcca
    46441 gaatgttgac taaaacaaaa gttcctatgt caaccgtcaa cagagacaat ctgtccgttg
    46501 caggacctga cagatttaga aatgttcaat ctgtctctta tgtattttaa atcaatactc
    46561 actaggagaa gtttcctaac ctcctaatag taatgctggt agtaacattg tcaaaactgc
    46621 ttcctcttag gaacaagcaa agcataacga gtgtaaaaat aaaatgtaga ctattgtaac
    46681 atatcattga ttatttcaag taaaaacgat aaaagaagta aacaaacact taaaaacaat
    46741 ggaaattagt cccaacttta agaaatgtat actataatgg aaattatcaa ttatttattt
    46801 ggaggaatat ccatgaaaca aaaaatcgca gttcttggtc ctggatcttg gggcactgct
    46861 ttagcacaaa cattggcaga aaacgggcat gatgtccgta tttggggaaa tgtacctgaa
    46921 cagattgatg aaatcaataa gtatcataca aatcagcatt ttttacctga tttgacgatc
    46981 ccagaatcga ttatcggata taaagaattg gcagaagcag tggataacgc agatgctatt
    47041 ttatttgttg ttccaacaaa agcgattcgt tcagtcgcac aggaacttat tggtaaaatg
    47101 aatactaagc caatcattat acatgcaagt aaaggactag aacaagatac gcataaacga
    47161 atctcagaag ttttggctga agaaattcca gaagaaaaaa gacaggcaat tgttgtatta
    47221 tctggtccta gccatgctga ggaagtggca gtacatgata tcacaacaat tacagctgca
    47281 agtattgatc aaaaagcagc agcctatgta caaaaactgt tcatgaatga ttatttcagg
    47341 atttatacaa ataatgatgt gattggcgta gaaactggtg cagcactgaa aaatatcatt
    47401 gcaattggtg ccggcgctat ccatggatta ggattcggag atgatgcaaa agcagcaatc
    47461 atgactcgag gattggcaga aatcagccgt cttggtgtcg cgatgggtgc caatcctctg
    47521 acatttatcg gtttgagtgg tgtgggggac ttgatcgtta cttgtacaag tgttcattct
    47581 cgcaattggc gtgccggtaa cttgttagga cagggacata agctggaaga agttctagaa
    47641 aacatgggaa tggtcgttga gggtgtggca acgacaaaag cagcagtaga gctagctcaa
    47701 cagctaggtg ttgagatgcc aattactcaa acaatctata atgttttgta taatggagaa
    47761 gatatcaaaa aagcagcaaa agaaatcatg ttacgtgacg gaaaaatgga gaatgaattt
    47821 tctcttcgtt aaatagatga tcaatagtta atcgt
//
LOCUS       DAJSBN010000019        44858 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJSBN010000019 DAJSBN010000000
VERSION     DAJSBN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.019"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            211..603
                     /locus_tag="ONT86_001163"
     CDS             211..603
                     /locus_tag="ONT86_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2351972.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            631..1971
                     /gene="glnA"
                     /locus_tag="ONT86_001164"
     CDS             631..1971
                     /gene="glnA"
                     /locus_tag="ONT86_001164"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCR2351973.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3164..4774
                     /locus_tag="ONT86_001165"
     CDS             3164..4774
                     /locus_tag="ONT86_001165"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCR2351974.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5066..5935
                     /locus_tag="ONT86_001166"
     CDS             5066..5935
                     /locus_tag="ONT86_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCR2351975.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6100..7371
                     /locus_tag="ONT86_001167"
     CDS             6100..7371
                     /locus_tag="ONT86_001167"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR2351976.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7368..8663
                     /gene="rho"
                     /locus_tag="ONT86_001168"
     CDS             7368..8663
                     /gene="rho"
                     /locus_tag="ONT86_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR2351977.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8762..9025
                     /locus_tag="ONT86_001169"
     CDS             8762..9025
                     /locus_tag="ONT86_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCR2351978.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9470..9919
                     /locus_tag="ONT86_001170"
     CDS             9470..9919
                     /locus_tag="ONT86_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2351979.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9976..11394
                     /locus_tag="ONT86_001171"
     CDS             9976..11394
                     /locus_tag="ONT86_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2351980.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11394..12254
                     /locus_tag="ONT86_001172"
     CDS             11394..12254
                     /locus_tag="ONT86_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR2351981.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12428..13201
                     /locus_tag="ONT86_001173"
     CDS             12428..13201
                     /locus_tag="ONT86_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2351982.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13348..13713
                     /locus_tag="ONT86_001174"
     CDS             13348..13713
                     /locus_tag="ONT86_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCR2351983.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13861..15069)
                     /locus_tag="ONT86_001175"
     CDS             complement(13861..15069)
                     /locus_tag="ONT86_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2351984.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15088..15366)
                     /locus_tag="ONT86_001176"
     CDS             complement(15088..15366)
                     /locus_tag="ONT86_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2351985.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15520..16215
                     /locus_tag="ONT86_001177"
     CDS             15520..16215
                     /locus_tag="ONT86_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR2351986.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16657..17454)
                     /locus_tag="ONT86_001178"
     CDS             complement(16657..17454)
                     /locus_tag="ONT86_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCR2351987.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17451..18635)
                     /locus_tag="ONT86_001179"
     CDS             complement(17451..18635)
                     /locus_tag="ONT86_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR2351988.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18660..19907)
                     /locus_tag="ONT86_001180"
     CDS             complement(18660..19907)
                     /locus_tag="ONT86_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR2351989.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20122..21867
                     /gene="dnaX"
                     /locus_tag="ONT86_001181"
     CDS             20122..21867
                     /gene="dnaX"
                     /locus_tag="ONT86_001181"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR2351990.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21887..22201
                     /locus_tag="ONT86_001182"
     CDS             21887..22201
                     /locus_tag="ONT86_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR2351991.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22226..22822
                     /gene="recR"
                     /locus_tag="ONT86_001183"
     CDS             22226..22822
                     /gene="recR"
                     /locus_tag="ONT86_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR2351992.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22886..23530
                     /locus_tag="ONT86_001184"
     CDS             22886..23530
                     /locus_tag="ONT86_001184"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR2351993.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23545..23874
                     /locus_tag="ONT86_001185"
     CDS             23545..23874
                     /locus_tag="ONT86_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCR2351994.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23888..24826
                     /gene="holB"
                     /locus_tag="ONT86_001186"
     CDS             23888..24826
                     /gene="holB"
                     /locus_tag="ONT86_001186"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR2351995.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24862..25686
                     /locus_tag="ONT86_001187"
     CDS             24862..25686
                     /locus_tag="ONT86_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCR2351996.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25679..26026
                     /locus_tag="ONT86_001188"
     CDS             25679..26026
                     /locus_tag="ONT86_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCR2351997.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26095..26967
                     /gene="rsmI"
                     /locus_tag="ONT86_001189"
     CDS             26095..26967
                     /gene="rsmI"
                     /locus_tag="ONT86_001189"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR2351998.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27076..28197
                     /gene="dinB"
                     /locus_tag="ONT86_001190"
     CDS             27076..28197
                     /gene="dinB"
                     /locus_tag="ONT86_001190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR2351999.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="ONT86_001191"
     CDS             complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="ONT86_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCR2352000.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="ONT86_001192"
     CDS             complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="ONT86_001192"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR2352001.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKNQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31656..32450
                     /locus_tag="ONT86_001193"
     CDS             31656..32450
                     /locus_tag="ONT86_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2352002.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32440..34482
                     /locus_tag="ONT86_001194"
     CDS             32440..34482
                     /locus_tag="ONT86_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2352003.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34763..34912
                     /locus_tag="ONT86_001195"
     CDS             34763..34912
                     /locus_tag="ONT86_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCR2352004.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34923..36437
                     /gene="dltA"
                     /locus_tag="ONT86_001196"
     CDS             34923..36437
                     /gene="dltA"
                     /locus_tag="ONT86_001196"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCR2352005.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36434..37639
                     /gene="dltB"
                     /locus_tag="ONT86_001197"
     CDS             36434..37639
                     /gene="dltB"
                     /locus_tag="ONT86_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCR2352006.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37684..37917
                     /gene="dltC"
                     /locus_tag="ONT86_001198"
     CDS             37684..37917
                     /gene="dltC"
                     /locus_tag="ONT86_001198"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCR2352007.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37920..39188
                     /gene="dltD"
                     /locus_tag="ONT86_001199"
     CDS             37920..39188
                     /gene="dltD"
                     /locus_tag="ONT86_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCR2352008.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39329..40411
                     /locus_tag="ONT86_001200"
     CDS             39329..40411
                     /locus_tag="ONT86_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCR2352009.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40411..41214
                     /locus_tag="ONT86_001201"
     CDS             40411..41214
                     /locus_tag="ONT86_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR2352010.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41284..42024
                     /locus_tag="ONT86_001202"
     CDS             41284..42024
                     /locus_tag="ONT86_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR2352011.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42035..43039
                     /locus_tag="ONT86_001203"
     CDS             42035..43039
                     /locus_tag="ONT86_001203"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCR2352012.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43084..44781)
                     /locus_tag="ONT86_001204"
     CDS             complement(43084..44781)
                     /locus_tag="ONT86_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352013.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14420 a         7827 c         9341 g        13270 t
ORIGIN      
        1 ataaaaacac taataaagga tagtacagta taataaaaaa taaaaggtat ctggtacgaa
       61 aatcaactag ataccttttt tgtgttctta tttttttgtt attttataaa aaatagagat
      121 ctcatgttag aaaacctaac atatgcagtt gacaatattt tttttaattg gtacacttta
      181 gccaacttat aaaacgaaag gggacgtctg atgggagaaa aggaattgcg ccgatctatg
      241 tccgtatttc caattggtac agttatgaag ctgactgatt tgtctgctcg gcaaattcgc
      301 tattatgaag aacaagattt agttcatccg gaacgaagcg aaggaaaccg acgtatgtat
      361 tcgctgaatg atattgatat gttgcttgag attaaagact atctttcaga gggattgaat
      421 atggctggga tcaaacgtgt atacgaaatg gcacaggcga aaaaacaaga agcaaaaaac
      481 aagaagccgt tgacagatca tgacgttcga caaatttttc gtgatgagat tttggcgcaa
      541 ggtgggttaa gcaaaacagg tcattatcag tcgcaaggat tgcaaagaca attctttgac
      601 tgaataaatt aaaaaaggaa gagggatacc atggtaaaga aacagataac aggtgaagaa
      661 atcaaacgaa tcattgagga agaaaacgta cgatttttac gattgatgtt cacagacatc
      721 ttaggtacga tcaaaaatgt agaagttcca gtgagccaga tcgacaaagt attagaaaat
      781 aaaatgatgt ttgacggttc ttcgattgaa ggatttgttc gaattgaaga aagcgatatg
      841 tatttgtatc cagatctctc cacttggatg atcttcccgt gggaaagtgc tcatggaaaa
      901 gttgctcgtt taatttgtga tatctataat ccggatggga caccatttgc cggcgatcct
      961 cgcgggaatt tgaaacgagc attgaaagat atgcgcgacc ttggttttac ctctttcaat
     1021 ttaggacctg aaccagaatt cttcttattt aaattagacg aagacggtgg gatcacaacg
     1081 accttgaatg ataaaggtgg ttattttgat tttgcaccaa cagatttagg tgaaaattgc
     1141 cgccgtgaca tcgtgttgga attagaaagt ctaggctttg aagtggaagc ttcacatcat
     1201 gaagtagcgc caggacagca tgaaattgac tttaaatatg cagatgtggt agatgcttgt
     1261 gataatatcc aaaccttcaa gctcgttgtc aaaacgattg cacgcaagca tggacttcac
     1321 gcgactttca tgccgaaacc gttatttggg atcaatggtt ctggtatgca ttgcaatatg
     1381 tctttgttca atgatgaagg aaatgttttc tatgataaag acggtgaatt ggaactaagt
     1441 gaaacagctt atcatttctt aggtggtttg ctgaaacatg ctcgtgccta cacagccgtt
     1501 tgcaatccaa cagtcaattc ttataaacgt ctcgttccag gttatgaagc gcctgtttat
     1561 gtcgcatgga gtggccgtaa tcgttcgcca ttagtacgtg taccagaatc tcgcggttta
     1621 tccacacgtt tggaattgcg gtcagttgat ccatctgcaa acccatactt ggcaatggca
     1681 gtattgttgc aagcaggact agacggtatc cgtaatcaga tcacaccgcc agcagctgtt
     1741 gaccgtaaca tttatgtaat ggatgaggaa gaaagagaag ctgcacatat ccaggactta
     1801 ccttcaacta tccataatgc tatcaaagaa ttgcgtaagg ataatgtaat gattgacgca
     1861 ttaggacaac atatcttctc aaattttgtt gaagcaaaac gtctcgaatg ggcggcattc
     1921 agacaaactg tttccgagtg ggaaagagaa caatatctag aactttatta aaacaggaat
     1981 accgtgaatt agaggctgtg aaatcagcgt tttgacctga gtattagaga aactaacgga
     2041 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattgaac
     2101 accgtttagt ggagaggttg tgaaaaagct gccctgcaac aagtaataag aatagccaag
     2161 caaaaacagc ttttcttgtt tttgaattgg ctgaacttat taccgcagat gcaagctttt
     2221 gaacaccgtt taccaagagg ttttgaaaaa gacgtttagt ttcgagtatt aagaaaaatt
     2281 ttgtgaaaat ggctttctac atttttgcat aaatttggct taataccgag aaactggctt
     2341 ttgaataccg tttatccaga ggttgtgagc ctaccattta actccgaatc acaagaaata
     2401 atttcaaaaa cagcttctca tgtttgatgg atattgtgac ttgtgaccga ggagttggca
     2461 ggagaacacc gtttatttag aaataaggag gcacgacttt tgtcgcatct ccttattttt
     2521 tacttatgtg attcgttacc ttcaatgaat ccctacaagg cacttttgtt gaaaaaaagg
     2581 tcatctttga aaaaattagt ggaattgttt tgtttttgat attatttaat agaaaagata
     2641 tgccaaaaat ccgagaactg tggactacgt tttttacgta aaggaggtaa ataagggtga
     2701 aagaaaaaga ttgtttctac gataagctat ataaactgtt gctgcaagta ggaaaaagta
     2761 gttctttgtt aggtgggata ttatctatca taatgtttgt tttatttttt tatctattat
     2821 ttcacttaaa atttttcagt aacgattttc tgatttgctt aggtttaacg ttagctatgt
     2881 ttatatcaag cttattatgt gggttaaatt ttttttgcac tcataaagga cttaagaaat
     2941 aatttctcaa aggatatcga aaaagaaaaa attgtaagcg gatagtattc atttgagtac
     3001 ttgactttcc cttagtcagc ttgtattata caccttgggc acccttttag agggtcagaa
     3061 gtttacagaa atttcggctc cctattctta ttgaagaata gggagctttg tttattgtct
     3121 gagacttttc ctgcacataa attaaaaagg agtttttgaa cttatgacaa aatatatttt
     3181 tgtgaccggc ggtgtcgttt catcaatcgg aaaagggatt gttgcagctt ctttaggacg
     3241 cttattgaaa aaccgcggct taaaagtaac tatccaaaaa tttgatccat acatcaacgt
     3301 ggatccgggg acgatgagcc catatcagca cggagaagta ttcgtaacag atgatggtgc
     3361 tgaaacagat ttggatctgg gccactacga acgttttatt gacatcaatt tgaacaaata
     3421 ctccaacgtg acgacaggaa aaatctattc tgaagttttg cgtaaagaac gtaagggcga
     3481 atacttggga gcaactgttc aagttattcc tcatattaca aatgagatca aagaaaaaat
     3541 catgcgagca gctaaaatga ctgattcaga tgtgatcatt acagaagtag ggggaacagt
     3601 aggggatata gaatctttgc cattccttga agcacttcgt caaatgaaag cagatgtcgg
     3661 tacagataat gttatgtata tccatacaac attgattcct tacttgaaag ctgctggaga
     3721 aatgaaaact aaaccaacgc agcacagtgt aaaagagcta cgtggtttag gtatccaacc
     3781 gaatattttg gtggtacgta cagagcaacc tgtttctcaa agcgtgaaaa acaaattggc
     3841 acaattttgt gatgtcgaac cagaagcagt tatcgagtcg ccagatgtag atacacttta
     3901 ctccatccca ttagttttac aagcacaagg aatggatcaa attgtttgcg accatttaaa
     3961 actagataca ccagaagcag acatgacaga atggaaagaa ttagaagaac gtgtcttgaa
     4021 cttgaagaaa aaagtacgga ttgcgcttgt cggtaaatat gtcgagttac cagatgcata
     4081 tatctcagta gttgaagcat tgaaacattc tggtttcgcc tataattcag acatcgagat
     4141 cgattggatc aaagcacaag aattaacgag agaaaatgta gaagaacgtt tgaaagacgc
     4201 agatggaatc ttggttcctg gtggatttgg tgatcgcgga gtagaaggga aaatcgaagc
     4261 gattcgttat gctcgcgaaa atgacgttcc gtttttaggt atttgtcttg gtatgcagat
     4321 ggcttgtgtc gaatttgcac gtaacgtcgt tggattagaa gatgcgtcat cagctgaaac
     4381 gaatccagat acagcaaaca atattattga tttaatggct gatcaggaaa acatcgagaa
     4441 tttgggtgga actttacgcc ttggattata cccatgtaaa atcaaaaaag gaacgaagac
     4501 ggctgaagca tatgatggag ccgatgttgt gcaagaacgt caccgtcacc gctatgagtt
     4561 caataacaaa tatcgtcaat tgtttgaaga acaagggcta gtcttttcag gtgtctcacc
     4621 agataaccgt ttagtagaaa tcgtagagct gccagagaaa aaattctttg tgggatgcca
     4681 attccaccca gaattgattt ctcgtccaaa tcgcccgcaa aaattaatca aagcatttgt
     4741 cggagcatca ttagcagatc acgaaacaaa ataatttttg aacttgatag aaagagaaaa
     4801 gtagcaagag aaggggataa gcaattttct tgtcactttt ctctttttct gatttgttat
     4861 tatttgtagg aaaaaagata gaaagcaaaa gacttttttc gattaaagcg ctattggata
     4921 ttttattttt tgcaaaaaac ttcttgaaaa ttttcatttt cattgatttt agtagaaaac
     4981 taccgaaatg tttggtaaaa tagggtcgtt ggttaagaat tgtcttaaca aatttagttt
     5041 tacaaaactt aggaggaatt ttattatgcc agtagtatca ggagctgaat ttttaaaagc
     5101 tgctcgtaaa ggcggttacg ctgtcggcgg attcaacaca aacaacttgg aatggactca
     5161 agcaatcctt gaagcggctg aagcaaaaca agcaccagta cttatccaaa catcaatggg
     5221 tgcagctaaa tacatgggtg gttacaaaat ctgtaaagat ttaatcactg atttagttga
     5281 agcaatgaac atcactgttc cagttgctat ccacttagac catggtgatt acgaagctgc
     5341 tttggaatgt atcgaattag gctatacttc agtaatgttc gatggttcac atctaccatt
     5401 tgaagaaaac ttgaaattag ctaaagacgt tgttgaaaaa gcacatgcaa aaggtatctc
     5461 agttgaatgt gaagttggtt caatcggcgg agaagaagac ggaatcatcg gtaacggcga
     5521 attagctgat attgaagaat gcaaacaaat ggttgctaca ggtatcgact acttagcttg
     5581 cggtatcggt aacatccatg gtcaatatcc agaaaactgg aaaggattag cttttgacca
     5641 cttacaagca attgctgatg ctgtaggtcc agacatgcct ttagtattac atggcggatc
     5701 aggtattcct caagatcaaa tcgaaaaagc tatctcaatg ggtatctcaa aagtaaatgt
     5761 taacactgaa ttccaattat catttgctgc agctactcgt aaatatatcg aagctggtaa
     5821 agataaagaa ggtaaaggat tcgaccctcg taaattatta gctccaggta aagcagcaat
     5881 cgttgaagat gctaaatctc atatcgactg gttcggttca gctaacaaag cgtaagcatc
     5941 cacataacag atacgatata taggaatgga gaacgacttt tgaagtcgct ctccattctt
     6001 ttttacttaa aacggcagac catgctcgtt tctttttttt atctatgata aaatacaagc
     6061 atatccatga aaaaagatta ataaacaagg tggaaacaga tgaaaaaaat cgtaatcaaa
     6121 ggaaaccgac ctttagctgg tgaagtaacg atcagcggtg caaaaaatag tgcagtcgca
     6181 ttgattccag ctgcaatctt agctgattcc ccagtgatct tggaaggtgt gccggacata
     6241 caagatgttc attcattaat tgaaatttta gagatcatgg gcgcaactgt ccatttttcc
     6301 aacaacatac tagaaatcga tcctcgagaa gtcgtatcga ttccgatgcc gcacggaaaa
     6361 atcaatagtt tgcgtgcttc ttattatttt atgggaacac tgcttggtaa atttggcgaa
     6421 gcggtagtag gcctgccagg tggctgttat ttaggcccta gaccgattga cctacacgtc
     6481 aaaggatttg aagctctagg tgctgaagtg acaaatgaac atggtgccat gtatttacgg
     6541 acagaaaata aaggattgcg tggcaatcgg attttcatgg acgtggtttc agtcggagca
     6601 acgatcaatg tgatgttagc tgctgtaaaa gcaaaaggac agacagtaat tgaaaacgct
     6661 gcacgagaac cggaaatcat agatgtggca actttactga ataatatggg ggcaaaagtt
     6721 cgaggagcgg gtacagatgt gatccgtatc gaaggagtcg aaacattaca tggctgtcgg
     6781 catttcatga ttcctgaccg catcgaagca ggaacctatc tagcattagc agctgcagta
     6841 ggaaatggta tcaaagtgaa aaatgtgatc tttgaacatt tggaaagctt tatagcaaaa
     6901 ttgcaggaaa ttggtgtcca tatgaaaatc agcgaagatg aaatcgaagt ttacccctcg
     6961 aaagaattaa aacccgcaaa catcatgacc tatccttatc caggatttgc cacagatctg
     7021 cagcagccac ttactgcgtt actattgatg actacaggca cttcagaaat catcgatacg
     7081 atctatgcga aacgtgtgaa ccatgtgccg gaactagcac gaatgggcgc tgatattacc
     7141 gtggaaggaa atatgatcat cgtgaatggt ccaaataaac ttcacggcac agaagtcgtg
     7201 gcttctgatc ttcgggcagg tgcttgttta gtgattgctg gactattggc agaagggaca
     7261 acgacgattt acaatgtaga ttatatctta cgcggttatg atcatattat cgagaaacta
     7321 acagccttag gggcatcaat tgaaatgata gaggaagtgg gagctgaatg agcgattatt
     7381 tgacaatggc agaactagag aacaaaacgt taaaagaagt ttatagttat gccaaagaat
     7441 ttaaaattcc ttattacagt aagatgaata aaaaggaatt atctctagct gtgattcgtg
     7501 cacaagcaga gaaacaagga ttttattata tggaaggaat attggatatc gttggtcaag
     7561 atggttacgg ttttttacgg ccaatcaatt atggaccaag tgcagaagat atctatattt
     7621 ctgcctcaca aatcagacga ttcagtttga gaaatgggga caaagtggca ggaaaagccc
     7681 gtccacctaa agaatcggaa cgttattatg gtttgatgca cgtggaaagc gttaatggaa
     7741 aagatccgga agaagcaaaa gaacgtccgc attttccagc cttaacacct ctttatccgc
     7801 aaagacagct tcaactggaa acaacaccgc aaagactatc tacccgaatg atcgatatct
     7861 tttctccaat cggttttggt cagcgtggat tgatcgtcgc accgccaaaa gcagggaaaa
     7921 cttctgtatt aaaagaaatc gcaaatggta tcaccgaaaa ctatccggaa gtagaattaa
     7981 tcgttctact gattgatgaa cgaccggaag aagtaacgga tctagaacga agcgtgaaag
     8041 gcgacgtggt atcttctaca tttgatctgc agccgcaaaa ccatactcga gtttctgaat
     8101 tagtattgga acgcgcaatg cgtttagtcg aagataaacg agatgtcgtt atcttgatgg
     8161 atagtattac tagacttgct cgggcctata acttagtcgt tccaccaagc ggacgtacat
     8221 tgagtggcgg gattgaccca gccgcattgt ataaacctaa gaaatttttc ggggcagcaa
     8281 gaaacatcga agaaggtggc agtttgacga ttttagcgac cgctttagtc gatacaggaa
     8341 gccgtatgga tgacgtgatc tatgaagaat tcaaaggaac tggaaatcta gaattacaac
     8401 tttcacgtga attatctgaa cgtcggatct tcccagccat cgatatcaaa aaatcaggta
     8461 cccgaaaaga agaattattg atgccaagtg aagaattaga agaaatttgg aaacttcgaa
     8521 aaaatatgac tggagattca ttagaatata cggaacaact gatccgcttt ttgcgtaaaa
     8581 caaaaaacaa tcagcaattt ttcaaagaat ttcaagatgt ctcctttggt aaaaaaaatc
     8641 cgaccagaaa tctgaaaaga taattgcaac cttgactgaa acatgttaag atgttaatgt
     8701 tgtaaatcga caaatcaact ctggttcagc aaatggagca gggcaaagga gcgaaaaaat
     8761 catgaaagaa aacatccatc cagattacca cccagtagta ttcatggatt caacaactgg
     8821 tttcaaattc ttgtcaggtt caacaaaaac ttcacaagaa acagttgaat gggaagacgg
     8881 taatacatac ccagtcatcc gtgtcgaagt aacttctgac tcacatccat tctacactgg
     8941 acgtcaaaaa ttcacacaag cagacggacg tgtggaccgt ttcaacaaaa aatacggtct
     9001 caaagacgca aacgctgcgg aataataaaa acgctgataa ctcaacgttt agcagactat
     9061 ctgataggga tagtctgttt ttttatgttt tttgccaatt taaaacttcg tttgatgctg
     9121 aaaaagtcgt aggatcataa tgcacaaaac aatgaaaaac tagtatatag ttttaattat
     9181 ccacttgata aaaacacctt atattaacga aatgaagagg ggttatttcg aagtaaaaaa
     9241 ccatttacct aaaattagaa gctaagcttt cctttcaagg aaggcttttt gttttgttat
     9301 agacttgcga ttgtttgtga gctagatcgt tcattttaat gacttataca cgcggatgat
     9361 gggtcttttt cctctgttca ttcataatat tgaacggtac agctagcgat gctatactga
     9421 cctcagttcc aagaaaacgt taacattttt tgtgggggag aggtttttta tgaggtttga
     9481 tcaattgatc gcagaagaac tgattgtttt cgatccatcg atagattcaa aaggatcatt
     9541 atttgagcga ataggggatg tgttgaaaaa aacaaataag gtaagaaatg ttcaaaaagt
     9601 cgttaaggca ctattcaaac gagaggagga gatctccaca ggaatagaag atggatttgg
     9661 gattccccat gcaaaaagca aatatgtgac aaaaccagca ctgtgttttt ttcatacagc
     9721 caaattaacg gactatagcg ggatagatga aatgccaatt gaatatattt ttgccatcgt
     9781 agttcctgaa aaatcagcag atatgcatct agagattctc agcacactct ccagacgatt
     9841 gatgaatgca gattttagaa agggaataaa agaagccact acgccagcag atatcatcag
     9901 acagattagt caagaatgaa aatagtagag tctttgtccg agtatcgata gtgaagaaaa
     9961 aggaggaaaa taatcatgaa aatcgttggt gtaactgctt gcccgactgg aattgctcat
    10021 acttatatgt cggcagaaaa attagaagta acagcaagaa atctaaacca tcaagcaaaa
    10081 ttcgaaacac aaggtgtaaa aacagaaaat cagttatctg aacaagaaat caaagaggca
    10141 gatgcaatca ttcttgcagt agataaagaa atcgaattgg accgatttgc cgggaaaaaa
    10201 gtaaagcgtg tctctacaag ccgagcaatc aaagaaccgc aagtagtaat agacgaagca
    10261 ttgagagata ttggcgtatt cgttgtgtct aatgaaaaag aaccagctgc aaatgagaaa
    10321 aagccgacaa tctataatca tttcatgaat ggtgtgaatt acatgcttcc atttgtgatc
    10381 gcaggcggaa tcatcatcgc actgagtttt gcctttggga ttacagcggc tgatcccaac
    10441 tcagcagact ataatgtgtt agcagctgca ttttcaagga tcggaggaga tacagcgttt
    10501 gccatgatgg ttcctgcgtt aggcgcgggg attgctacat cgattgctgg aaaagcaggt
    10561 tttgccccag gaattgttgc cggattactg gcctctacag gtggttcagg ttttcttggc
    10621 ggaatgattg gtggcgtgct agcagggtat atctgtgatt ttctggcaaa taaaattagc
    10681 gtaaaaaaag aattttctgc tatttatcag ctgattgtcg tccccttttt atcgatctta
    10741 attattgggc ttttgatggt atttgtgatt gaacaaccga tcacatgggt gttggatgct
    10801 ttgacaaatt ggctgaatag cttgggaaat acatccgggc tattgttcgg attacttatc
    10861 gggatcatga tggcagctga tatgggtggg ccgatcaata aatcgacttc gactttctcg
    10921 atcggtttga tgtcagctgg agtaaatgca ccgattgctg cttgtatggc tgcaggaatg
    10981 acacctcccc taggtattgc tttagccact ttattattca aacataaatt taccaaagaa
    11041 gaacggactt caggtcaatc atgctggatt ttaggcgctt cgtatattac agaaggtgct
    11101 ataccgtttg cggtatctga cccgatccgc gtgataccta gtttgatgac aggttcagca
    11161 gtcgctgcag ggatttccat ggcagtagga gttacttcaa tggcacctca tggagggatt
    11221 tgggtgatgc taattccaaa tgttatgaac aatttaccta tgtatgtctt ggcaattgca
    11281 tcaggtacag tcactacagc ctttatgctt ggactattga aaaaaacagt gaaagagaaa
    11341 aaacaagaca agtggacgaa aaatgaaaat ctaacaaatg aggtggaagc gtaatgttat
    11401 atacaatgaa agagttattg aaagtggcta aagagaatca atttgcagta ccagccttca
    11461 atatttgcag ctatgatatg atgaaagcaa tcatcgaaga agcagagaga ttagaagcac
    11521 cagtaatctt agaaattcat cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg
    11581 cagtaagagc ctatgcttat acaagtaaac tgccgatcgt tatccacttg gatcatggcg
    11641 gtacgataga agatgtctta cgggcgattc gaaacggcta ttcttccgtg atgatcgatg
    11701 cgtctttgaa atcattcgac gacaatgtac agattacgaa aaaagtggtt gcgttagctc
    11761 atgcggtaaa tgtctcagtg gaggctgagc ttggaacgat tgggaataat ggttctgctg
    11821 aaggaggtgc tgaaacaatc atttatacag atcctgatca agcgaagatt tttgtgaaag
    11881 aaacaggaat cgatacactt gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa
    11941 acagaactcc aaaattaaat atcgacttac tgaaagaatt gaaccaacaa atcgatcttc
    12001 catttgtttt acatggcggt tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat
    12061 acggagttgc caaagtgaat ttgagctcag atcttaaaag cgtctttttc aatgctttga
    12121 gagaaatatt gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat ccagcagcta
    12181 ataaaaaggt acaagaagtg gtcaaacata aaatggaaat tttgaacaca gtaggcaagg
    12241 ctagtttata ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag tcttgtcata
    12301 ccctcttaac cacacacgat gttcagaagt agttttttcg aaaaatttct tcttatttct
    12361 cgaagccaat cacttctgtc acaacctctt ttttcctgtt actatataaa gtaaatgggg
    12421 tgattttatg ttgaatgaag tggagaatag acagcaggag attgtgaatt atctaaaaaa
    12481 caaccaattt gcaagagtca ccgatttgat cgagttagta aattatagcg aagccacgat
    12541 taaacgtgac cttgtttttc tggaaaaaag tggattgatc cgtagagttc gcggtggcgc
    12601 catgcttatt gacaatcgaa aaattgacgt gccttacctg atgaagatca ccaaattaga
    12661 agaagaaaca gaaaaacgta agattgcaga tacagcttct tctttgattc gagatgatat
    12721 ggtcttattc ttggattcca gtacaacctc tttacactta gtcagaaacc tagcaagatt
    12781 tgatggtttg cagatcatta cgaatggcat cgtcacagct gctatgttga gtgaatatac
    12841 aacggcaaaa gtatctgtat tgggaggggc aattgttccg aaacgagcaa caatcaacgg
    12901 ttcaaaagcc tataacgatg cattgtcata ttatgcggat ttagcgataa tgagttgtcg
    12961 cgggctagat ttcagccaag gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa
    13021 agcttttaga aaacaatcag accggcttgc cgttttagtt acacagcaaa aaattgcaca
    13081 taaattcatg catcaagcca ttgcttttca tgaaatcgat tatctggtga caagtaaaga
    13141 actgacaaaa gaacagtatg cgaaagtaaa ggaacaccat atcgaatgtc tgtacggata
    13201 aattattacg tgaatgttct aagtggcttc ctcattttta gataggacct tatctttgct
    13261 cgcttctctc agccgatggt ataataatga aaaggttatc ttgttagata atcttacttt
    13321 tttatccagg agaaaggagt gcagaaaatg aaacatgtga aggcgttggt agtgaaggca
    13381 atcatgatct gggcgattct gtgggtcgtg ctgacaggtt tatacggtgt tagctttatg
    13441 gattcaacga ttgttggggt aattattgta gtcatgatct atgtgctagg ggatctgttg
    13501 atcttacgta aagtagggaa catcgcagcg acgatcgcag atgctggttc agctgcagtt
    13561 atcttgtggc tctatctata ttttatgaat gatacagtcg atatttggat gaaagtattg
    13621 attcctgctc tattgatcgg tgtggcagaa tggttcttcc acaagtggtt gctaagtcaa
    13681 ggcatcgtac cagatgaacg gaaaatggaa taaatcaaca gttaaactat tcttatcgat
    13741 aaatgaaagc tttaaaaata aatagatgtt taattaagaa taatcataaa ctatttttac
    13801 tcaaaaaacc gctcagatta ttttcagatc tgaacggttt tttttatcta ttcatcaaga
    13861 tcaaatttca tttttcaatg attcaatcaa ttcgccctct gcttcatgac tatgttttcg
    13921 gccattaaca agatagagtg ctgtacatat caatggaatg actccggcaa tcacatacaa
    13981 tcctctaaat gaagtaagag attttagcat ccccatcaaa taaggaccaa cgcctaagcc
    14041 taggtctaaa ccaataaagt aggtagataa agcaatactg attcgatgtt cgcggacgat
    14101 ttttaaacaa acagcctgtc catttgacat aaacgtacca taacctaggc cgactaatgc
    14161 tccagagata agcaatgtca cactattcgt cgttacactc agtaaaaata atcctgctgt
    14221 taagaataaa tagctaggat acatcacgta ttgttctcct ttgacatcga aaatccttcc
    14281 agtcaatgga cgtgtaaatg tgatgaccaa agcatatacg acgaagaaaa atgacccagc
    14341 agcaactaag tggattactt ttgtataaga agataaaaat gataaaacac ttgaataaga
    14401 aagacccatc aaaaaggcaa ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt
    14461 gatattccat gagcgaagag atattttttg ttcttcagat aatggaatat tttttacagg
    14521 aaagacaagg caggcaatcg taacaaagaa gaccagtaca attgaaaacc agataatgaa
    14581 acgaaaatca gtcatattca gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa
    14641 actagtactt aatccataat aattgatgcc ttcccctttt ttagatgcag ggatatatgc
    14701 agtaacgata gcatttgtag ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg
    14761 aactaaatac aatgccccga tagttggcgt ataaagatat gctaatgttg aaaggagata
    14821 gaagatcgcg ccgcctctta aggtaaactt ccgaccaaat agttctaact gtttccccat
    14881 gaataagcgt gccagcaatg ttccgatgat atagatccca gaagcaaaac ctgcttggct
    14941 caagctggcg tgtaaggtgt cttgcgcaat gactgccatg atgaccatca acagataata
    15001 aatcaggtag acaataaaat tgatcaatgt gattccaata aaatatttat taaataattt
    15061 cttttccatg tatgttatac attcccctta agctgaatat gcttttaatt tttgtatttg
    15121 aacttctaca tcgtgataat tggatggata gcgatcaaca agttggcgaa taaataaatc
    15181 tcttgtagac ataaacagat ttaaaaattc ttcgcattcg tttgttggat tctcatccac
    15241 tgctttgatc aattcgatcg agttttgata cgcatcattt aaaaagacta aagaagcttt
    15301 catatcaaat cttgtttgat atgctgtttc taatcgatta atgattgtgt tcatccagta
    15361 attcatgttt tttccctcta ttcttaaaaa attttacttc tgcagtataa cgaataaaaa
    15421 aaaggaaaaa aatcgcataa gtgatggaaa aaggtactta acaaatgtta atatcattag
    15481 taaaatacct ttggacttta aaaagtttta aagtactgta tgatcttttt agatgaaagt
    15541 ttggatgggg gagcatcatt ggatagtaga gtcttaaagg aatatttgga taaaaatgat
    15601 tttccgatta tacgcaaagg atatcataaa tatttgacat tcgaagggat ggaagattcc
    15661 catacgtata tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa tgatgggaga
    15721 gaatttaacc ttcgctatat caaagatctt gaaatcgtct ctcttttaaa agacgaatat
    15781 tcacaattca tcgattcgcc gtataatatt cgagttgaat cagaatatgc agagttttat
    15841 cgaatcaatc gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct acaacactat
    15901 gtaaaagagt attaccgcta tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta
    15961 ttaaacggaa agctgggagc tgtttgtacg caactttacg aattatatga tttattcggt
    16021 gtgcggacag aagaagggat gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa
    16081 ttttgtggca tctcaactgc tagcagtgta aacagaattt taaaacaact gaaaaaagat
    16141 ggtattatca ccgttaaaga ccatcatatc gttattttga atatggactt gttagaagac
    16201 aatattatct tttgatgaac aaagaggttg tgaaatcagc gttttgacct gagtattaga
    16261 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
    16321 agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    16381 ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagga
    16441 gttggtagga gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat
    16501 aagaagagcc aagtaaaaac agtctcttat gtttttaact tggctggact tatcaccgta
    16561 gatgtaagct tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac gatttttgtc
    16621 acagcccttt ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc
    16681 aaaagtcccg ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg
    16741 gcttttgggg attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt
    16801 taatttgata ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata
    16861 acgcgaaaca gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat
    16921 gatgatttct actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac
    16981 acgataatgt cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc
    17041 ttttgcagca ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga
    17101 atcgattctg ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc
    17161 acgctgtccc atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct
    17221 taagtaatta tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa
    17281 gactcgtcct ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg
    17341 atctgttgtg aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac
    17401 cggccagcca acacttacta gcaaaagaaa gaataaaaaa aactttttca tcatatcaag
    17461 taacctccga tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc
    17521 aattttttct ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg
    17581 tagtagtaga cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt
    17641 aatacgtaat tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt
    17701 gtatatccat ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga
    17761 atctgtgatt tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa
    17821 tactggcttt taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc
    17881 ttttgttgcg tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct
    17941 gctcttgaaa cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg
    18001 cggataactg taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa
    18061 gggaaataca cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca
    18121 aaccattttt tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca
    18181 aaaggaagta ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga
    18241 ttatggcagt agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca
    18301 cagttgggac aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta
    18361 tctgaaaaac cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc
    18421 tgttcttggg ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg
    18481 ccagccatac gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag
    18541 atattcaggc agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg
    18601 ccgcaattag gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat
    18661 taattaacgg ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg
    18721 ttggaaagat ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg
    18781 ttttctacag gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg
    18841 caaactggac atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct
    18901 gcttgttgtt tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt
    18961 gcattcattc ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg
    19021 gctgcttcca taccacgtgc aatggaacct tgcacgtatc cttcacgtac gctaggatca
    19081 cctagcatcg ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa
    19141 atgctggcta cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc
    19201 aatgcagtat ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt
    19261 tgaccatctg cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa
    19321 tactgctcat tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta
    19381 taaaataaaa tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat
    19441 tctgcgttat agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc
    19501 tttatttctt ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat
    19561 ttgaatcggt caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga
    19621 gctgcgtcat ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca
    19681 ccatcgacca aaagcatcat catgtttgga tagacatgga cgactgttcc gtctgttaaa
    19741 acatcttctg ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct
    19801 tttgtcatga ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct
    19861 gccagaccgc cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc
    19921 tctgattcag ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa
    19981 acttctcgga ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa
    20041 aagccgagaa ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt
    20101 ttttaaaaaa gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag
    20161 cgtttcgaag acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt
    20221 tcccacaaaa cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt
    20281 gcagcaaaga ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc
    20341 aacgaatgcg aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc
    20401 gatgctgcca gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat
    20461 gcgcctacaa aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca
    20521 ggagctttca atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc
    20581 ttagcaacga ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt
    20641 gattttaaac ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa
    20701 tcgactatca gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg
    20761 atgcgggatg cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact
    20821 ttagatgatg ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg
    20881 agtgcttgcg tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca
    20941 gccggcaaag aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg
    21001 atgtatcagc aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt
    21061 acagaattgg cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc
    21121 gatactcaaa atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca
    21181 gtcaaacttg cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat
    21241 gatcagatag taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa
    21301 ctacgcagta atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat
    21361 aaaaaacaga caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa
    21421 gcgacaagac agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta
    21481 tctgtcactc agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga
    21541 ctagtgattg cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg
    21601 cagctttcgg tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt
    21661 tatattacaa gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa
    21721 gagggaacag aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct
    21781 caagaacaag aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc
    21841 gaagctgtta cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat
    21901 gggcaatatg caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc
    21961 acaagagctg ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc
    22021 ggtttttaca ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc
    22081 tgatgatatc gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca
    22141 aattgatgtg gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta
    22201 gttttaaact gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga
    22261 cagctacatg aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac
    22321 gatcgatatg aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg
    22381 cgatttgcat ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg
    22441 tagaaatcct tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat
    22501 ggcaatggaa aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc
    22561 accgatggaa gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca
    22621 tgatgcagat gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac
    22681 agctatgtat ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca
    22741 tggtctgtct gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt
    22801 cgaaggaaga cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag
    22861 cagcacaaga gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga
    22921 tggtgcagga aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa
    22981 aacagaaatc attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc
    23041 agtgatcctt gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc
    23101 agcagcaaga agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat
    23161 cgttttatgc gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat
    23221 cggcgttacc gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt
    23281 taccctttat cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca
    23341 aaatcaaatc gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc
    23401 ttacttaaag ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag
    23461 ctttgaagaa gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt
    23521 tgaaaactag gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac
    23581 agcaaccgtt tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg
    23641 acaggcggct tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt
    23701 gtagaagaag cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct
    23761 acacctgtta cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa
    23821 gtaggcggtg ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag
    23881 ctgagctatg aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct
    23941 tcaacggagt tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg
    24001 aacaggaaaa catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa
    24061 agacaatctg ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc
    24121 agatgttttg gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt
    24181 gcagacggaa ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga
    24241 agctgaaaaa atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc
    24301 tggagatttt ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca
    24361 atctcgttgt caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt
    24421 gcaagaagag cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt
    24481 aggaaaagca gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta
    24541 tcaatggttc gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa
    24601 attggtaaaa tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt
    24661 ttattatcag gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca
    24721 gataaaccag ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag
    24781 ttttcaggct gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga
    24841 tgatgatcaa ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt
    24901 catatctatt attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc
    24961 gaatctcagc aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc
    25021 aatgaccttc ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa
    25081 aaagacaaaa aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa agaaaaagtg
    25141 cagttgcgtg agttggaaat gaaaatggtc aaagccgaat acacatttga tcgaaataaa
    25201 ttgatttttt ctttcacagc ggacgggcga attgacttta gagaattggt aaaagatctg
    25261 gccgctgtat ttcgtactcg tattgagctt cgacaaattg gtgtccgcga tgaagcaaag
    25321 ataataggcg gaattggtcc atgtgggcgt ccattatgct gttcttcttt tttaggcgat
    25381 ttcgtgcctg tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa
    25441 atttccggtt tatgcggacg cttgatgtgc tgtctgcaat atgagagcga agaatacgag
    25501 acagcgaaaa aagaactccc tgactttgga aaagtaattg ctacacctga tggtaaaggg
    25561 aaagtcgtag ggttgaatct gcttagccgt atcgttagta ttcggctgac tggacgggaa
    25621 aatcctgtcg aatatgaatg ggatgaattg aaagaattta tccaagtagg tgagatgaat
    25681 ggataagaga tcattatacg acggattgaa tcaattagaa tcagagttga gaaatacatt
    25741 aacagaactt gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa atacaacttt
    25801 ggagatggaa aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc
    25861 tccagctgaa aatgtgaagc aggaactttc taagtctcgt atgaatcttg aaaaaattta
    25921 tgaagaagga ttccatgtct gctacgattt atatggatca agaagggaca atgacgagcc
    25981 ttgtgcgttt tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa taacaagagg
    26041 agactggagc ggagaaatac tgctccagtc tttttattaa ggagattgtg acagatgtat
    26101 aatcaaaaaa gtttcaaggg aaatccggta tatggggatt tatatcttgt accgacgcca
    26161 attggaaatt tagaagatat gagctacaga agtgttcgta tgctgcaaga atccgatctt
    26221 atcgcaagtg aggatacgcg taatacacaa aagttattga atcattttga aatccataca
    26281 ccccaaaaaa gtttacatga acataattat aaagaacgtg tccctcaatt gattgctttg
    26341 ttaaaagaag gaaaaacgat tgctcaagtc agtgacgcag gaatgccttc catcagcgat
    26401 cctggacatg agctagtttt agcctgcata aaagaaggga tttctgtgat ctctatccca
    26461 ggacctacag ctgggatgac ggctttgatt gcttcagggc tagtgcctca accattttta
    26521 ttttatggct tccttccacg taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa
    26581 gaacagacag ttacgctgat tttttatgag tcaccttatc ggatcactgc tacattgacg
    26641 aattttttag aaatttttgg aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc
    26701 catgaagaat accttagagg aagtacggaa gagctactcg cttatatcga agaacatcca
    26761 gtaaagggtg aatgttgtct gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa
    26821 gttgatgaag agttgggaac gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa
    26881 aaaccaaatg ctgctatcaa agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc
    26941 taccgtcagt accatgaatt agactgagat agtataagtt gtctataatg aaagactaat
    27001 gaggaaatca gctactaact tttcaaatct cttgaacgcg aacgaatgtt cttgtataat
    27061 gaataagagg tgaggatgat ggagctgcaa tttcctttac ataatgatac atcaagaaaa
    27121 atcattcata ttgatatgga tgcttttttc gcttcggttg aagaacgaga tcatcctgag
    27181 tttcgcggac atccattagt aattgccaga caccccagtg acactggtgg gcgtggagta
    27241 gtgactacag caaattatga agcaagaaaa ttcggaattc attcagcaat gagtgcacaa
    27301 aaagcgtatg agctttgtcc gcaagccatc tttaagcctg gcgaccatca taaatacagc
    27361 gatatttcca aacaagtacg ggaagtattc tatcgttata cagatattgt ggaaccagtt
    27421 agtatcgatg aagcgtatct tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc
    27481 aaaatcgccc gaatgatcca agcagatatt tgggagagtg tccagctaac atgttctgcg
    27541 ggagtaagtt acaataagtt tttggccaaa ttagcatcag actatcaaaa acctagagga
    27601 atcacggtag ttgcccctca agacgctgtg ccatttttaa aagctttgcc aattgaaaaa
    27661 tttcatgggg tagggaagaa aactgtacct agaatgcatg agctagggat ttatacagga
    27721 aaagacctat atgaatgcac agaaatgatg ttgattcgaa attttggaaa aatgggctat
    27781 tctctttatc gtaaagtcag aggaatccat gattctccag taaacgttac aagagagcga
    27841 aaatctgtgg gaaaagagca tacttatggg cagccgttgc aaacggaaga agcagtgctg
    27901 actcagttgc gccaactagc tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat
    27961 gggaaaacag tagttttgaa ggtcaggtac acggactatt ctactgtgac caaaagagtg
    28021 acattgccag aatatatcta taaaaaagaa gcactctttt atcaagcaag ccttatatgg
    28081 gaggaaatct tgggagtaga aaaaggaatc cgattgttag ggattacttt gacgaatctg
    28141 gacccgatga cttatgaaaa catcgtcctt ccattatggg aaaatcaaga aatataaaaa
    28201 cagctttgag agttgaaacc ggaaatccgt tttctctccc aaagctgttt ttatatcaag
    28261 tcttgctttt tgatttgttc ataggtttct gttgcatccg tacatttttc ccaaatcaca
    28321 acttgattgg attctaaaga tttttggacg tcaatgatcc gctggttgct acttccgcga
    28381 aattgtagtt ttaggtcacg tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt
    28441 tgcgaaagga gttctaattt atcttctgaa tcttgtagca attcatcaaa ggcatagcct
    28501 gtccatgacc agatatcttt tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc
    28561 agacaaacat ctgtattcaa aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa
    28621 tcgttgcgca agtcagcgat gatttgttct tctaattcag gtgtgaatgg tttgccatat
    28681 cggaaatttt gtgcagcttt gttgaagcag ccttcgcagg caaataagca gccactaacg
    28741 taaagactgt tgcggacacc ttcaccatcg acaaaattaa acggtttgta gtcagcaata
    28801 tagttttggc tgtagtcttt tgctaaccat tcttttggtt ttggatttct cataaaaaaa
    28861 cttctttcat ttaaggttca aagatatagc tattgaagat aaaatctact cgaccacata
    28921 aataagaaca aactgaccta aatccattgt tcaagaaagc ttgttcttat tttgatagag
    28981 ggcgttcaaa gatacaaagc catcgaaaga gggtggtgaa aaaagacttg tcatagcctc
    29041 ttattctgaa taaacggtgt tcaaaagctt gcatctgcag taataagccc aactaaacga
    29101 aaaatatgag gaactatttt tgtttagcta ttcttattac ttgatgcagg acagcttttt
    29161 cacaacctca tttcatgtgt ttcacacgtg aggaaatttc tttatggcgg ccatgtacca
    29221 tcggacgagc ttgcggattt cctaaatagc cacaagtacg tttgactaca tcacatgttt
    29281 ttggatcatg atttccacat tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac
    29341 cttcgaaacc acattgatag caatgatcga ttggcgtatt cgtaccaaga tacccgattt
    29401 tgtcatatgc aaaatcccat acagcttcta aagctttcgg attttgttgg agcatagggt
    29461 attcacagta gtggatgaat ccacctgaac aatattttgg ataatccttc tcaaattcca
    29521 atttttcaaa tggcgttggg tttttacgca catcatagtg gaaactgttt gtataatatt
    29581 ctttatctgt gatattttcg atgatgccga atttttctgt atccaaacgg cagaaacggt
    29641 cagttagact ttcacttgga gttgaataga cactgaaatg ataaccatat tcatcccccc
    29701 atgcatctgc attagctttt aattctttga cgatatcaag tgtgaaagct tttgcttctg
    29761 ggtttgtttc ccattcgcca ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc
    29821 ctaacgatac agtagctcga cggtttttga ataattcatt gacagaatcg gtacgtgaaa
    29881 gacgtttccc gaaagaacca tacatatata aaattggcgc atttgctgga atggcttctt
    29941 tacaacgttc tacacgatag agaagcgcat ctttcatgat ttctaagcgg tcagacagta
    30001 actgccagaa tttattctga tctccttttg attcgatcgc gattcttggc aaattcaatg
    30061 taacaacacc taaattcatt cgtccgacat ttacttcttg accattttcg tctttccacc
    30121 cttgcaagaa tgaacgacag cccataggta ctttgaaact tccagttaat tcaacgattt
    30181 tatcgtaatt cagaatatca ggatacattc ttttcgttgc acattccaat gctagttgtt
    30241 tgatgtcgta attaggatct tcagggttta agttcacacc ttttttcaaa gtgaagatca
    30301 gtttagggaa gatagctgtc cgcttttcac tccctaatcc attgattcga atctgtaaaa
    30361 ttgctttttg aatttctctt tcaaaccagt tttctcctag tccaaatcct aatgaagtga
    30421 aaggagtttg tccatttgaa gtaaataagg tattgatttc atattccaaa ctttgcatag
    30481 catcgaagat atcttttttg gtttttgctt ttgcatattc tttttggcgt tctggcgaat
    30541 cgatccattc ttccgcgtct tttagatgtt ttttatagtt caattctgca aaaggagcaa
    30601 gcaattcatc tgttcggtcg gcagaacagc caccatattg acttgacgct acattggcaa
    30661 taatctgtga gatttgtgct gtggcggtct ggatagattt tggcgattcg acttcggcat
    30721 tgccgatttt gaacccattg ttcagcatgc ctttgaaatc aatcaagcag caattcgtca
    30781 tcggtgtata aggatgataa tccaaatcat ggtaatggat atcgcctttt tgatgcgcat
    30841 ttgctatatg tggtggcaac attttcagtc cgattgattt tccaacgatc ccggctgtca
    30901 gatctcgctg tgtattgaaa acattactgt ctttatttgc gttttcgttc acgacagttt
    30961 ggtccttgtt gattagcttt tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa
    31021 aatctcgttt ggtacgataa ttgatgtact cttctgctaa ttcatattcg tttttttcta
    31081 gtaatatatg ttccacgatg ttttgaatct catagatttt gacattttca gcaaaccggc
    31141 ttttgatttc ttggacaacc aagtctgcaa tcatttgatt tttttcataa gtaagtggtg
    31201 ataatgagcc gtgaattttc ttttctgctt tgatcagtgc ttcattgatc ttttgttcat
    31261 cgaaatcaac atggcggcca tcgcgtttga tgactttcat ctgggatgtc tctggctgct
    31321 gagtaacgac agttttttct tcataattta ctttcattat cttcaactcc tgctaaattg
    31381 ttcttgtcta tcataaaaca aaatcgggga aggagcaact aaatatcggc tatatatagt
    31441 attcaatgtg ctgtataatt ggatttctat actatatgta gtgtatattt tattgtgaaa
    31501 aaaggtgaaa acttgtagct ggtggatttg ttttttgcat atttaatttt gctgaataaa
    31561 ataattgaat aaaatttttt attcagtcca agtaaaagtg acaaaaacat aagagcaaat
    31621 tattcgtaga tatgctacta tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag
    31681 aattggaaaa gatgatcgaa gtgaaacatc taagtaaaag ctacggcagc aaactttctc
    31741 ccgtcaaagc cttagataat atctcattca cagtagaaga aggcgagttt gttgggatta
    31801 tgggcccaag tggtgcagga aagacaacgc tgatgaatat cttgtccacg atcaatttgc
    31861 ctacgatggg cagtgtctcg atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc
    31921 tcagtgactt tcgtcgaaaa aaactaggat tcatttttca agagttcaat ttgatcgata
    31981 ccctaaatgc aaaagacaat atcctattgc ccctagcagt tgaacggatg actaaagagg
    32041 aaatggaaaa gagagtaaga catgtagctc aattgttgaa tatagaagaa ctgctcaaac
    32101 ggtatcccga tgaactttct gtcggacaga gacaacgaat tgcagctgct agagcacttg
    32161 ttgttcagcc acaagtgatt tttgcagatg aaccaactgg ctctttggac tcgaagtcgg
    32221 ctacagaatt gttgaactac ttgaagacca tgaatcaaaa agaaaaagca acgatactgt
    32281 tagtcaccca tgatccatat acagcaagct actgcgatag aattttattt attaaagatg
    32341 gtgtgatttt ttctgaagtt gtcagaagag ggacaagaag agagttcttt gagaaagtaa
    32401 tagacatgca ggcgacgatt ggcggaggag gaaaaatgaa tgctgtataa actagccgtt
    32461 aaaagttttt ttcggcagtc acgaggctat cttgtttatt ttttcagttt gactttatcc
    32521 gtaatgatct attattcctt cagcgcaatg acctacgatc aatctttagt cagaagagca
    32581 agtcaagata cttcgataga tggtggattg agttttggtg gatttatcat tactgttgtt
    32641 ttgctttttt tcgtattcag tgccaaccga tttttcttga atagaaggca aaaggaaata
    32701 ggcatctatc agctttttgg tatgaataaa ctgcagatat caggtatata tgtgttagaa
    32761 atcatgataa ttggattgtt cgcctgtatt tctgggatat tactcggtat tattttttcc
    32821 aaactctttt caatgatttt ggttcgaatg atggatatgg atcttactag tccatttttt
    32881 atctccattc catcagtagt agatacttta tttgcttttt tcatcatcct tttagcagta
    32941 tctgtatatt caatatggaa aatatggcgc tatccaatga ttcgaagttt tggagaaaaa
    33001 gaacaagcag atagttcaac aatgcgcttc aggacaaggc accgattact cgcagtcatc
    33061 gggttactac ttttaacgag cggatatttt ggagctagtc attttaggga aatcacttca
    33121 tggatgatca gttatcatta tcttgataca ttagttttct tttttccttt tttaattttt
    33181 ttcgtttgtg tggtcggtac ttatttgttt ttctgttata catttcggtt atttttgatc
    33241 atattgagta aatggaagat ttattactat ggtatcaatt ttttgataat gggtaacacg
    33301 caagttcatt tgctcaaaag ttggcgtaca aattctctga ttacagtcat tctcggaatt
    33361 tctttaatga caattggcgg cacggctagt atctctacta ttttatccta ccaggagaaa
    33421 gtttcctccc cgatggacta tcagtttgat gttgagacag agaaaaaaat caagccgatt
    33481 ctagcagagg aagggcagaa gataactaaa gaaatcacct tgcattataa agtcattggg
    33541 agtatctata gtcaaaatct cgaagaacct atgtttcaaa ctgctaatct gatcacggaa
    33601 acacaatatc aagccttcag aaaagttaat ccagatttgc cgaacatttc tttgaaatca
    33661 cctggtcata ctgttttgct tgatcaaatg caaactcttt ttcggaatat ttcattatat
    33721 ggatctagta ttggactgcc agataatcaa gaattgaaga ttcaacaaat gcttccaagt
    33781 gtattaggtg acgaaacaat ggtatatcta agtcctgtac tgattgtaga tgataccgtt
    33841 tttgaaaaag caaaaggtgt ggattatcaa gtagtcaatg tagatgtatc aggtggaaat
    33901 aatgaaaaaa tagatgaacg actgagcaat gacctgtcga taaagtggct caatgctgtt
    33961 tattattctt atgatatcgt aaatggaagt ctacaaggag aaattagcac aaagaaactt
    34021 ccggatgacc aaatgaagga agcacaattt agccaagaaa gctctcgttt gaatttctct
    34081 tccagctatt cgaaattgcg tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca
    34141 ctatttgtcg ggatgatcgt gattattgct actggtagta ttttgacagt ccggcaatta
    34201 gcagaagcag aggaagaaaa agaaaattat cacctattga ctaagctggg gattccacaa
    34261 agaaggatca gacggatgat cttcgagcaa aacgcactta ccttctttcc acccatgata
    34321 ttagggatca ctcacgcagt atttgcaatc aatgtcttca ctcagtatat aaaaactgcc
    34381 gattactggt tggcttatct tgtgagtggc ttattgatcc ttatctatct tttgctttat
    34441 gtcattacga gtcagttata ttgtcggatt atcgaggaat gatatacgga ataagacaag
    34501 ataataaata gaactaaatt ttgaatacca tttatttgga ataagaggtt gtgacacgat
    34561 ttttgtcaca acctctttat tttttatatt ttttaaaagt acgagaaatt ataaagaaaa
    34621 aagcagtgat ttgatgtgct atttttataa aagctatgtg acaaaactgt tagtaaaaaa
    34681 acggtttctg taagctagac acatgtttct ttttgtcaaa attagttaca ctttgtgtga
    34741 ggttatatga ggagggaatc caatgaacaa atggcgttct aatccaaaat tgaattattg
    34801 gggaaaattc atcattcgaa caatttttta tagtgcagta ctgctgttcc tgatttatct
    34861 gtatcactat aaaaatattc aaggaggaac ttttatctat aacgaatttt aggaggaaga
    34921 gtatggaaat caaaacgatt attgaagcaa tcgatgattg gggaataaat gaacctgatc
    34981 gagttgctta tcaggcggat gagacgcata cttttggtga attgaaacaa gcatcagacg
    35041 cacttgctta ttatttagaa aaaaaggtag aaggcgatgg accaattgtc gtttttggca
    35101 atttggagtt tgaaatgatt gtctcatttc taggggttgt caaatctggc catgcctatt
    35161 tgccgattga agagcataca cccaaggagc gtatcctatc gatctttcgg gtggctaaac
    35221 catctatggt catcagtatc ggggactgga tagaagatat tccttcggtt cctgtcataa
    35281 caaaaaaaga atttcaagaa atcaagaaga taccagtagg attttatccc agaaatagcg
    35341 taaaaggtac agaaaatttt tatattattt ttacttctgg tactactgga gaaccaaaag
    35401 gcgtacaaat cagtcatgat aatctagtca gctttgttca atggacgatg gaagactttg
    35461 ggatcaagcc tggtatgcat ttcttagccc aagcgccatt ttcttttgat ttatcggtat
    35521 tttctattta tcctgcatta gtttctggcg gaatgctgaa gccgttaaat aaagcagttg
    35581 ttcaagactt tcgtcaatta ttcgcgacat tgccagcctt gaagttgaat gtgtgggttt
    35641 ctactccatc atttatggat atttgcttga tggaacctac attcaatgga gaaaatgtgc
    35701 cggaattgga catgttttta ttttgcggag aagaattgac gaaaaaaact gcagaaagtc
    35761 tacttgatcg attcccaaat gcacggatat ataatactta cggtccaaca gaagcaactg
    35821 tagctatttc aagtattcag attgaccagc atgtactaga tagttatgac cgactaccga
    35881 ttggctacgt aaaaaaagac acgaaagttt ctattgtcca agaaggtaaa cctgtttcaa
    35941 aaggagaaac gggtgaaatc attattgcag gaccaagcgt atcaaaaggt tacctgaaca
    36001 atcccgaaaa gacagctgct gcgttctttt gtcaaaatga cttagcaagt taccatacag
    36061 gggatgcagg tagactagat gaatatgggc ttttatttta tgaaggacgt atggatcagc
    36121 aggtcaaatt acatggatac cggattgaac tcggggatat cgaacattat ctgctccaag
    36181 acaaccgaat caaacaagct gtcgtcgtac caaaatacca aggaacaaaa gtacagcagc
    36241 tggttgcttt tgtcgtatta gaaaacaaaa cgaaacagcc ggattttcag ttgactaaat
    36301 ctatcaaaga acaattacta gaagttgtaa tggattatat gattcctcag aagttccatt
    36361 atgtcgatct gctgcctcag acagttaatg gaaaaatcga tcgaaaaaaa ttaacagctg
    36421 aggtgaatcc tgaatgatgt tacctcatat gatcccttat gcagagccgt tttatttttt
    36481 tcttttagcc attgcgcttg taccgatcgt tctatcttta ttgatatggg aaaaaagatt
    36541 accagtttat caatcagcag tgacactttt ctttttattt gtcagctttg gaggtaatta
    36601 ttggcaacag ggagcggcgc tgattgggta tgttttgtgg cagacgatac ttgtatgggg
    36661 ctattttgtt tatcggcaaa ggaaaaatca gacaggatgg ttttttgccg ccgttatttt
    36721 agcgataatt ccactgtttt taacgaaagt gactcctttt tttgaacatg gaaaagcatc
    36781 gttacttggt tttttaggaa tctcttattt aacctttaaa tctgtacaaa tgatcatgga
    36841 aataagagat ggcatgatca aaacatataa gccatttcgt tatatccagt ttctgctgtt
    36901 ctttccaaca atctcttctg gaccaatcga tcgctatagg agatttgaaa aagatcagct
    36961 ccatccgcct gttcgtgata aatatgttga tcttttggga aaaggcgtcc aaaatatttt
    37021 tgtcggtttt ttatataaat tcattatcgg ttattatttt ggtcaagtac tgcttcctgt
    37081 agtgggacgc attgccttac agcatggtgg tatttcatgg gcattagtag gttatatgta
    37141 cgtgtacagt atgtatttgt tttttgattt tgctggctat agcttatttg cagtcggtac
    37201 aagctacatg atgggatatc agacaccaat caatttcaat aaaccttttt tatcatggaa
    37261 tatcaaagaa ttctggaatc gttggcatat gacgctgtcc ttttggttcc gtgattatgt
    37321 atatatgcga ttgatgttct tcatgatgaa gaaaaaattt tttaaaagca agatcgtaat
    37381 gtcaaatatc ggttattttg ctttgtttct tctaatgggt atatggcatg gattgacgtg
    37441 gtactacatc gtatatggac tgtatcatgc ttgtctgatc tgtctgacag atgcatggat
    37501 ccgatttaag aagaagcata aagagcagtt gccttctaac aaatttacgc atggcttagc
    37561 cgtatttttg acatttcaag cagtatgttt tagcttcctg attttttcag gatttttaga
    37621 taaacttttt tttagctaac gaatagtgac atattaattt atataacaaa ggagaaaagg
    37681 aaaatggaag aacaaatttt atcaatctta gaagaaataa caggaacaga tgaagtaaga
    37741 aaaaatcgtg atattgatct atttgatgaa gggttgcttg attctttagc atctgttcaa
    37801 ttattagtcg agctagacgg agagctggat atccaagtcc ctgtatctga attcgagcga
    37861 gaggattgga gcacaccgaa taaaatcatt caacaagcaa ctgcattgaa gggttgagta
    37921 tgaaaaagaa actttttgct attttcggcc cggttgtttt agctgttggc ttactgtttg
    37981 tgttttttac ctcctcctat caagtagata tctctaatcc agaaacgatt agacgtgcct
    38041 cttcttccat gtcagtgaac gtgttgaaag gagatgcgat aaaaaattat gcattatctg
    38101 aaaaacaata tattcctttt tttggctcaa gtgaattaag cagaatcagt ccatttcatc
    38161 cgtctgttct ggcagaaaaa taccagcgaa actatcgtcc atttttgctg ggagcacccg
    38221 ggacacaatc gttaagccaa tatatgatga tgcgttctgc aggagatgct atgaaaaaca
    38281 agaaagtcgt ctttattatt tctcctcagt ggttcgttaa aaatggtgta aaaactgatt
    38341 actttaatac gtattattct gaacttcaaa catacgactg gttgttttca atgaagaaag
    38401 taacgcctgc tgatcgctat ttagctagaa gattattaac tttctcaaaa gtaaaagaga
    38461 atgatacgtt gacggcgatt ctgcaaacga tcaaaaaagg gaagctacct ttacctgaat
    38521 ctttgaatca gctgcgcagc caatggaaca tgctgaagag agaggatgaa gtattcagca
    38581 atattgggtt gaaagatcga cagcaaaaaa tcgatcatga gtcaaaacga ttaccaaaac
    38641 aataccaaga aacagaatta tccattttag caaatcaaat tggagaacga gaaacgacga
    38701 ataatccatt cgggttgaaa aacgattttt atacgcatcg tatccgagcc cacgagccag
    38761 agctcaaaca gtcacaaaaa aattgggatt atcgtttttc gccagaattt tctgattttc
    38821 agctggtctt ggatcaatta gcaaaaaacc ataacgaagt attgttcatc atcccaccgg
    38881 ttaacgagaa atggagcgat tataccggac tatctcaaga aatgttacaa ggatttgcca
    38941 aaaagatcaa atttcaatta aatagccaag gattcaatcg aatagctgat tttgtcaatc
    39001 aagcgggtac caactatttt atggaagaca cgatccatct aggctggaaa ggctggttgg
    39061 ctgcagatca acagatcaga ccgtttttag aagaaaatca tattactgct agtaagtatc
    39121 atcttgacga tgcttttttt agcaaatctt ggcaacatca aataccagat aaactacaat
    39181 taaaataaag aaaacaggcg agtgttgctc gcttgttttc tttatttttg aatattctga
    39241 ataaaagata aaaataccaa gtttgcctga acaaaaatca taagcaatgg tataatcaca
    39301 atatgaatat agaaatgggg gaaaacattt ggatacaaaa gaagaacagc tacttattga
    39361 tttaacttct gcaaaaggag tacctggaaa tgaagaagag gtaagagaag tattcagaga
    39421 atatgccaaa ccattcgctg atgatatttt ttatgacggt ttaggcagtg tgattgccaa
    39481 acatggtgct ggcggaggcc ccaaggtctt tatttctgga catatggacg aagttggttt
    39541 tatggtaaca aaaataaccg aaaaaggatt cttggaattc caaactcttg gaggttggtg
    39601 ggggcaagtc atgcttgctc agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca
    39661 cggtgtgatt ggttccaaac caccacatgt tttaacacct caagtaagaa ataaacctta
    39721 tgaaataaag gatatgttca ttgatattgg cgcatcaagc caagaagaag ccaaagagtg
    39781 gggcatacgt cctggagata tggtcactcc atatattgaa tacaaacgaa tgaatggcag
    39841 taaatattta ctggcaaaag catgggataa tcgtatcggg acagctgttt ctctgcgagt
    39901 attagagaat ctatctaaag aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca
    39961 agaagaagta ggcttgcgag gtgctcgaac aagtacacac cttgtaaatc ccgatattgc
    40021 ttttgcttta gatactggta cagcaggaga cactccagga atgacaccaa aagaagcaga
    40081 ttcgatttta ggaaaaggcc cacagatttt gatttttgat gcatctatga tcccgcataa
    40141 aaaattattg aattttgtta ttgatgtagc agaggaaatg gatattccat tccaatatac
    40201 agtgatttct ggcggaggaa ctgatgcagg acaaatgcat ttgacaagag atggtgttcc
    40261 gtctgtagcc attaccgttc ctgttcgtta tctgcattcc catacatcag tgattcatga
    40321 agatgattat ttgaatacag tgaaactagt cacagaatta gtgaaacggc ttgatagtga
    40381 aacagttgaa aaaattcgga gttatgaata atgaataata gaatacctat ttctgaatat
    40441 cggaaaaaag cacgagtttc tttggaaaat caatggggaa tcaatacttg gcttgttttc
    40501 ttatctataa tatttacgag cgtcatccag aacttttttg acggtttcag tccttttccg
    40561 gaagaaagca tgcagcagca cttattgagt tttctagtcg ataatttact tttattcgca
    40621 ttcaactatg cattgtttta cattgcgcta gtagttgtac ggggtggcag accaaaaagt
    40681 aaccttttat tttcagtttt ccaaagaaag tattttggtt cgatacttgg catcaatttg
    40741 atcaattcag tcatcaactg gctactcaac agtttaatct ttctgcccat gtttttaatc
    40801 agcggagtga gtacttatat gcagctgctt ttcagttcaa atgcggtacc tacgggtgtc
    40861 atgccagaag attttcttga tatcagcttt gttttatcga tgatagggat gatttttgtt
    40921 tcagcactca tcatgagtat tgttggcggt ttattccaat ttgctgcatg gacaaagttc
    40981 gactatcctg atttgtcggt tattcaatgt ttgaaatatg gctggttttt attgaaagat
    41041 agaattggta cgtatatttt gttgcagctg tcttttatcg gctggtattt gctaggagca
    41101 atcgctttgt tcatcggtct tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa
    41161 ttttatgaac aagcacggat tgaaaaagga ggtccagacg cctattttac ttagcatgta
    41221 agcagtttat tcatcaaaaa aataactttt gtggtaggat aaatctttga gggggaatgt
    41281 acgatgaaat taactgtttt aggatgctta ggagcgtatc cttataaaaa tcaagggaca
    41341 acaggttatt tgcttcaatc tgacgggttc aatttgttga tcgatgcagg tagtgctacg
    41401 ctgatcaaat tacaagaaca ccttgatcca cttgatctag atgctgtgat ccttagccat
    41461 tatcatcatg atcatattgc tgaccttggt gtattacagt attactggca acttcatcct
    41521 aagagaaatc cggagacatt gctttcaatt tatggacaca caaaagattc cattcacttt
    41581 gaagaattga cgttggaaaa tgtctcaaaa ggacatcctt attttgaagc tgaagaatta
    41641 aaaattggtc cttttctgat tacttttatg gaaacaatcc atccagtagt atgttacgcc
    41701 atgcgtatcg tagaagaaac aacggggaaa gtattcgtat ttacaggtga ttcaggttat
    41761 ttggaaagtt tcgctgattt tgcgaaagat gctgatttgt ttctagcaga tacctatctt
    41821 tttgaaggaa atgaacatca tcatgcccat ttcacttcaa aagaagctgg agaacttgcg
    41881 aaatcagcta acgtaaagaa acttgtactt acacacctac ctcagtttgg tgatttagaa
    41941 caattgaaaa atgaagcgca aaaggcagca ggcaaaggga ttgatataga acttgctgca
    42001 gttgacaaag tgtttaacat ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca
    42061 cgacccaaga atcaatttag caattgaaca atatttgctg caagaaatgc ctttagatga
    42121 acctattttg ctcttttata tcaatgaacc ttcaatcatc atcggcagaa accaaaatac
    42181 gcttgaagaa atcaatcgag attatgtgga agaaaatggc attcatgttg tccgtcgctt
    42241 aagtggaggc ggagctgtct accatgattt tggaaacctg aactttagtt ttatcatgcc
    42301 tgatgatggg gattcattcc gtgactttgc gaaagtaaca cagccaatca tccaagcact
    42361 tcatgaattg ggtgttgctg gggcggaatt gaaaggccga aatgatctag tcattgatgg
    42421 catgaaattt tctggcaacg cgatgtatgc taccaacggt cggatgtttg ctcatggaac
    42481 aattatgttc gactctgata tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa
    42541 aatcgaatca aaaggaatca aatcgatccg ttctcgtgta acgaatatca aaccatttct
    42601 tccagaagaa aagcaaaaaa tgacaacaga agaatttcgt caagaaatct tgttgaaaat
    42661 tttcggcgta gagtcagtag aagaagtaaa aacgtacgaa ttgactgatg aagattggaa
    42721 aaaaatcaat cgaatctctg aggaatatta tcgaaattgg gattggaatt acgggaagtc
    42781 tccagatttc aatttctctc gccaaaaacg tttttctatc ggttcgattg aagtccattt
    42841 gaatgtctca gaaggaatga tcaaagaagc aaaaatcttt ggcgatttct tcggactagg
    42901 agatatctct gacgtggaaa aaaaattggt tggacagaaa tatgataaag catctctatc
    42961 aaaaatagtc agccaaattg atgtgaagaa atattttggt gcgatagaac cagaagaatt
    43021 gcttacctta ctttattgaa aataaaaaat agcggaatcc acatatatgg attccgctat
    43081 tttttataca ggattcgggt aatttcgaga gagttgaaca gtgatccgat caatgaaagt
    43141 atctttttcc tgcgttagtg ttcctgtgta acgatccgct acgtctttca tcatcattaa
    43201 gtcaaaacgt tcctgatcat ctaattcttc tgtgatgcct cgactaaata ggtttgtatc
    43261 gtaatgtgca ctatgttcgc aacaaatgac gaaatgttcc tcttgttcgt gtatcgacaa
    43321 ataaattttt cgatacagtg gattttcgat ggcatgagtt tctctaaagg aatgctctaa
    43381 agcgtggatc aaaagttgag ccagatcgtc atcgttgata ggaagttctt cgggaagatc
    43441 tgcttggaag gttatctgaa tattccgttt ggcagctaac ttctggtaac gcgctaagat
    43501 aagatttgtc acctggtggt tgcaaaaaga acacagcatt tcgaaatttt ttgcttcttc
    43561 taatatcttt tccacatagt ttttagcttt tgtttggtcc gtatgcatta aatcttgcag
    43621 attctccatc tcttggacaa attccaggcg aagtgtattt acttcttgga tatgctgtcg
    43681 aagctgctca taatgttgct ccaacaaatc atttttgatt tttaagaaat ctacttgtct
    43741 ccgatctttg tcggtattta agatgtgatc gattagattg tagctgataa taacgattag
    43801 aatgataata tataaaatcg tagaccaatt gatcgttgtt tgatagatac ctaatgccgt
    43861 ttggatgtaa atcaagcaat ggataatggc taccaagacg atccaaggtg tacaagctac
    43921 ataaaaacgg ttattcttat atgcttcgcc aatgcatgca agtgaagtga ctaaagtgct
    43981 tataacacta gcaatattag caatcggcat taattcagga agttctgcag gagtaaaagt
    44041 tgcataaagc agaacgccga taaaaatcaa aagttggaac gtacaaaaaa ttccgtacca
    44101 ttttttatag taaaacattc ggctccaata gtagcagatg atgaaagctg acgttaagat
    44161 aataaataaa tgagataaaa aagaatgaac gaatggttca aaaagaatac cacttaaaat
    44221 atcttctgat acagcttcaa atcctaatgc caaagcaaag caacttaaga gaataagtga
    44281 gtattcaaga cgtttttgtt tatacatcaa gtagatcgta tagaaaaagg tccctatagc
    44341 gataaaaaca gcgattatca tcgtcaatac ttgcggcact gattgtgaat aaataaaact
    44401 aatcaatggt ccagttgttc ctagataaac tttcggtggc agtccagcat agttctgata
    44461 aggagatgag actgtaaggg tcaatttttt tccgatataa tttgctggta aatctatcac
    44521 agccaatgag agtccgggat tgctttcttc ctgataatct ttccgttggt ataaaatctt
    44581 ttcgcctaga tatacagtaa tccattgatg gtttcctttg attagtaaat ttgcttcgtc
    44641 caacggtttt tcaagtgtcg tttccataat gaatgtttcg tctttatcca cggtaggaat
    44701 cgtacgagtg tacttgctgt caaacttaaa atcaggatca tcttttgtat agaaaaccca
    44761 cgaatctttt atttctttca tttgattgct gtccgcagga gaaaaaataa aataaaagaa
    44821 ataagcaatt cctgttaaaa ataacaatga taatataa
//
LOCUS       DAJSBN010000020        43560 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJSBN010000020 DAJSBN010000000
VERSION     DAJSBN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.020"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(199..2580)
                     /locus_tag="ONT86_001205"
     CDS             complement(199..2580)
                     /locus_tag="ONT86_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR2352014.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2570..3217)
                     /gene="recU"
                     /locus_tag="ONT86_001206"
     CDS             complement(2570..3217)
                     /gene="recU"
                     /locus_tag="ONT86_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCR2352015.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3281..3820
                     /locus_tag="ONT86_001207"
     CDS             3281..3820
                     /locus_tag="ONT86_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCR2352016.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3910..4329
                     /gene="gpsB"
                     /locus_tag="ONT86_001208"
     CDS             3910..4329
                     /gene="gpsB"
                     /locus_tag="ONT86_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCR2352017.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4351..4718
                     /gene="rnpB"
                     /locus_tag="ONT86_001209"
     ncRNA           4351..4718
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ONT86_001209"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4927..6093
                     /locus_tag="ONT86_001210"
     CDS             4927..6093
                     /locus_tag="ONT86_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCR2352018.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6139..7635
                     /locus_tag="ONT86_001211"
     CDS             6139..7635
                     /locus_tag="ONT86_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCR2352019.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7716..8399
                     /locus_tag="ONT86_001212"
     CDS             7716..8399
                     /locus_tag="ONT86_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2352020.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9044..10234
                     /locus_tag="ONT86_001213"
     CDS             9044..10234
                     /locus_tag="ONT86_001213"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR2352021.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10336..11289
                     /locus_tag="ONT86_001214"
     CDS             10336..11289
                     /locus_tag="ONT86_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR2352022.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11358..12707
                     /locus_tag="ONT86_001215"
     CDS             11358..12707
                     /locus_tag="ONT86_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2352023.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12890..13213)
                     /locus_tag="ONT86_001216"
     CDS             complement(12890..13213)
                     /locus_tag="ONT86_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352024.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13342..14505
                     /locus_tag="ONT86_001217"
     CDS             13342..14505
                     /locus_tag="ONT86_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR2352025.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14575..15924
                     /locus_tag="ONT86_001218"
     CDS             14575..15924
                     /locus_tag="ONT86_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR2352026.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15969..16400)
                     /locus_tag="ONT86_001219"
     CDS             complement(15969..16400)
                     /locus_tag="ONT86_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2352027.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16788..19430
                     /gene="alaS"
                     /locus_tag="ONT86_001220"
     CDS             16788..19430
                     /gene="alaS"
                     /locus_tag="ONT86_001220"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR2352028.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19630..20226
                     /locus_tag="ONT86_001221"
     CDS             19630..20226
                     /locus_tag="ONT86_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2352029.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20348..21496
                     /locus_tag="ONT86_001222"
     CDS             20348..21496
                     /locus_tag="ONT86_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR2352030.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21534..21947)
                     /locus_tag="ONT86_001223"
     CDS             complement(21534..21947)
                     /locus_tag="ONT86_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCR2352031.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22026..22457
                     /locus_tag="ONT86_001224"
     CDS             22026..22457
                     /locus_tag="ONT86_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCR2352032.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22556..22789)
                     /locus_tag="ONT86_001225"
     CDS             complement(22556..22789)
                     /locus_tag="ONT86_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCR2352033.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22972..24642
                     /locus_tag="ONT86_001226"
     CDS             22972..24642
                     /locus_tag="ONT86_001226"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCR2352034.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24789..25175
                     /locus_tag="ONT86_001227"
     CDS             24789..25175
                     /locus_tag="ONT86_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352035.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25162..25860
                     /locus_tag="ONT86_001228"
     CDS             25162..25860
                     /locus_tag="ONT86_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2352036.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25865..26329
                     /locus_tag="ONT86_001229"
     CDS             25865..26329
                     /locus_tag="ONT86_001229"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR2352037.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26326..27237
                     /locus_tag="ONT86_001230"
     CDS             26326..27237
                     /locus_tag="ONT86_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR2352038.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27472..28011
                     /gene="pyrR"
                     /locus_tag="ONT86_001231"
     CDS             27472..28011
                     /gene="pyrR"
                     /locus_tag="ONT86_001231"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCR2352039.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28025..29308
                     /locus_tag="ONT86_001232"
     CDS             28025..29308
                     /locus_tag="ONT86_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR2352040.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29321..30247
                     /locus_tag="ONT86_001233"
     CDS             29321..30247
                     /locus_tag="ONT86_001233"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCR2352041.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30266..31546
                     /locus_tag="ONT86_001234"
     CDS             30266..31546
                     /locus_tag="ONT86_001234"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR2352042.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31551..32627
                     /gene="carA"
                     /locus_tag="ONT86_001235"
     CDS             31551..32627
                     /gene="carA"
                     /locus_tag="ONT86_001235"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR2352043.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32632..35817
                     /gene="carB"
                     /locus_tag="ONT86_001236"
     CDS             32632..35817
                     /gene="carB"
                     /locus_tag="ONT86_001236"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR2352044.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35871..36647
                     /locus_tag="ONT86_001237"
     CDS             35871..36647
                     /locus_tag="ONT86_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCR2352045.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36644..37576
                     /locus_tag="ONT86_001238"
     CDS             36644..37576
                     /locus_tag="ONT86_001238"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCR2352046.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37573..38289
                     /gene="pyrF"
                     /locus_tag="ONT86_001239"
     CDS             37573..38289
                     /gene="pyrF"
                     /locus_tag="ONT86_001239"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR2352047.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38296..38985
                     /locus_tag="ONT86_001240"
     CDS             38296..38985
                     /locus_tag="ONT86_001240"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR2352048.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            38958..39227
                     /locus_tag="ONT86_001241"
     CDS             38958..39227
                     /locus_tag="ONT86_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352049.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39265..39906
                     /locus_tag="ONT86_001242"
     CDS             39265..39906
                     /locus_tag="ONT86_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2352050.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39949..40641
                     /locus_tag="ONT86_001243"
     CDS             39949..40641
                     /locus_tag="ONT86_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCR2352051.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40638..41540
                     /locus_tag="ONT86_001244"
     CDS             40638..41540
                     /locus_tag="ONT86_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2352052.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41698..43404)
                     /gene="efbA"
                     /locus_tag="ONT86_001245"
     CDS             complement(41698..43404)
                     /gene="efbA"
                     /locus_tag="ONT86_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCR2352053.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13947 a         7684 c         9472 g        12457 t
ORIGIN      
        1 aaatggataa agtatatgta atgataaaag aaaaatggat tttctaattt ttaagctaaa
       61 tgttagatca cacatgtata tgctggatag tcttataaaa aatcatgtaa tacagcttgg
      121 tacatgattt ttatttgtag ctgtttttta catagacaca aaaaagaaac cacgcttgta
      181 gacgtggttt cttttttact atctgtttcc agaagctgag gtgctgctgg atggtgtatg
      241 gtcatttgca gcatccgctc cgctagttgc tggctcactg gaagaagcag gaggagcaga
      301 tgaagacggt gtcgagcttt cttcggatga agaagaagga accgttgttg aagacgctgg
      361 ctcgcttgtt tctgctgtcg tgctttcttt tgaggactca gcagttgatt cgctttgact
      421 agtagagcta gagctttgag tcgttgtctc tgttgttgag cttccaggag tctggacata
      481 gctggaagat ggaatagttg ttgatggtgt aattgcattc gatctagctg tatattgatc
      541 tttgtagtac agttcaccgc ccacacgaat taatccgctt ggcatctccc agtctgtatt
      601 tgtcacgtta gctgaaacat actgcataag ctcgcgataa acatcagagg ctacgtgtga
      661 cgattctgaa gtgacaggtg tcattttttt attgtaacct gtccacacgg aaatcgaata
      721 atttggcgta tagcccgcaa ataggatatc tggatacacc ccagaagaaa taccaagttt
      781 tgcatactcg tcatctgtgt aattggatgt tccggtctta ccggcttgat ataacccagc
      841 gatttgcgca ttcgtaccag ttccttcagt gatcgtatct tttaaaatgt ctgtgatcat
      901 ataagctgtt tctggagaca tggctgtttt tccatctggc tcataagttt cttctgttcc
      961 gtcttggaag acgattttat taacatattg aggtttgtaa taagtccctc cattggcgaa
     1021 agccgcatag gctgctgcca tttttaagga agaagctccg tattttgtcc catcttgttc
     1081 ttccgtatta ctagagatcg cattcgattg atggatcgta ctgtattcga tcccaagatt
     1141 ttttaagaaa gaagccactt tatctgaacc gacttcattg aataatttga ctgctggaac
     1201 attacgggat aagtaaagtg cttggcgtaa agtaatggtg cccatatatt gattatccca
     1261 gtttcctaca ggagtcgaag tcccttcata gttatacggt gcgtcagtaa tagtttttcc
     1321 agtagaatat ttcaaatatt caaaagctgg tccgtaatct gtgacaggtt tcatcgtaga
     1381 accaaaatca cgggaagtat tcactgctaa gttgttgcct aaagttacgt cttctgcgat
     1441 gtgacgtccg ccaatctggg cttttacttt cccggtattg gtgtcgataa gggtggaggc
     1501 aacttgcatc tcgtcgtctg ggtaagagac atactgatcc gtgttgacga tatcatatag
     1561 tttcttttgg gcgtccagat ccaaattcgt ataaatctct aaaccgtctg tgtagacatt
     1621 cttatctgtt ttttcttgta cttcattgat gacttctttg acataattat cgacgatctt
     1681 agtattgtca tcggattgag ttagttcttg cagaccatcc gttaccggta cattaacagc
     1741 ttgatcatat tctgtttgag agattttttc gttttgaagc atcgtataca aaacagtatc
     1801 tcgtcttttc ttcgcctgat ctggataaac ataaggatca taggcggaag gggcttgagg
     1861 catcccagca agcaatgctg tttgcggtaa agaaagttct gataatttct taccaaagta
     1921 catctcagaa gctgtctcca tcccgtacaa gccgtttgac atatacactt tatttacgta
     1981 gtaggtcaag atctcttgtt tcgatttttt ttgttccaat cttacagcca tccacgcttc
     2041 ttgggccttg cgtttcagag tctgatcctc ggcactggtg gagaagaagg aaagtttgat
     2101 caattgctgt gtcaaagtac ttccgccttg cagtccgcca gaggtaaagt tagagagggc
     2161 agaaccgatg atccgaatag ggtcaacccc gatgtgttta taaaaacggc gatcttctac
     2221 cgacacgatc gcatcttcta atgttttcgg taattcattc gcagaaattt tttcacgctt
     2281 ttctgcgcct aaatcttcga atagttcgcc atcttgtgta tatagtttgg aagatacggt
     2341 agcgtctaat tttttatccg tcaattcggg cgcatctttt gcgtaatacc agaaaagtcc
     2401 tacacctgct aaaaagagga tacagaaaaa tgagagtatt cctaaaagga tcttgataaa
     2461 taatcctttt ttatgtgttc cagaggactt tccagaacct ttgccagaat tcttcttcac
     2521 actcttttta ggagtaggct gtttctgtcg tctagagctt ctagtttgtt catttgccat
     2581 tcttgctttc tccttcaata gataatgttg ataaatagaa gtcaaccgcg tccaaataag
     2641 gaatccgcgg agaaatacca gttttgatct ctattcctag ttcttcgatc aaagataaag
     2701 gtagtgattt gcgtcctttt tccttttggg cgtcccagta agcaatcaaa tgaacactat
     2761 tcaaataaaa acagcgcttc aaagacgaaa accataacaa gacaaagcag accccatttt
     2821 gttcgaggca tgctttcata tgttcgattt gatgggcatg gaagttttta aagggaaaag
     2881 atgttttgtt cttggtttct tttgcttcaa agtccaagta aaagcctcga tagacgccat
     2941 tatagtctgt agttgaggct tgggtaaagt aggcttcctt gatcacagca gcacttctct
     3001 taggatagtc tactttcacg atttggatag gggtaggctt tttatggata accgccaacc
     3061 cgcgggaaag ataatattta ttactttcat tgattgcttc ttcaaagcgc atcccccgat
     3121 tgccgaattc agtgatctga tttctttttt cttgaatttt ttcgacggga gaggcattct
     3181 tataataagg ttggccattt ggatagcgca ataccatctt taatcacact cctaggccac
     3241 attatagcaa aaaagcggat agaaagaaaa ggaatctgag atggataagt caaaagtaat
     3301 ctatgtgtct gggtatcgaa gtttcgaact cggtgttttt aaagaaaatg atcctaaaat
     3361 ccaagttgtt aaaaatgttt taaaaaaaga actccatcag ttagcagaag aagggctgga
     3421 atgggtactt gtcagtggga atttgggcgt cgaactatgg gctgtgcaaa tagtagcgga
     3481 attgaaagaa gaatatccgg aactatctct tggtattttg tatccttttt ctgattttgg
     3541 taataactgg aatgaaggga accaagaaaa aaaacaatta gcagaacaat tagcagatta
     3601 tgtcgaagct gttagccatc aaccctacca atcacctagt cagctaaaga atcacactcg
     3661 ttttatactt gagcatacag tgggctgtct gctgatctat gatgaagaat tccctggaaa
     3721 aacgaagtat ttttttgaag atgcacagaa ttaccaagca gatcatccct acgaaattcg
     3781 gctgatcagt atggacgatc ttcaaaattt tagtgaataa tcgatattgt gtttgattta
     3841 tcaacttttt tcctatacaa tgaaaaaggt agaaatgtta ttacgtaaat gaaaactaga
     3901 ggtgtaaata tggcgaattt ggtttatagt cctaaagaca ttttacaaca agaattcaaa
     3961 acaaaaatgc gcggctatga tcccgttgaa gttgatgaat tcttagacaa tatcatcaaa
     4021 gattatgaag cttacagcaa agaactcctt gctttacaag aagaaaacga tcgattgagt
     4081 gcaaaagtag ctcaattatc taaaactcaa ggagcagctc aaacacgcgt gcaacaaaca
     4141 gaggcaccaa agagcgcagc agtgaccaat tttgatatct taaaacgctt gtcaaatctt
     4201 gaacgtgaag tatttggtaa aaagcttgat caacaagcaa gtgcagtcaa accagcacaa
     4261 cctaatccaa ataactacac aaatgcagac acaagcttag atgataatga aaaaacacgt
     4321 caattttaat ttttgattaa tgaatttgcg gttttcgggt aatcgcggtc tgacttgatc
     4381 aggctgagga aagtccatgc tcgcacaggc tgtgatgcct gtagtgttcg tgcttagcga
     4441 aaccataagc taaggtactc tttggagtaa cggcaggaaa acaagctaag gcttcggcta
     4501 tgcttaagta tccttgaaag tgccacagtg acgaagcggc tggggaaacc catccggtgg
     4561 aacgcggtaa acccctcgag cgagcaaccc aaacaatggt aggggcgctt tttgtctgga
     4621 agaagaacag gacaaaaagg cagtataact gcagatagat ggttaccgct ttgttttttc
     4681 tccctggaaa agcaaagtac agaacatggc ttatagaaaa ccgcaaacat cagggaatta
     4741 ggtcgtgaca aaagtaatgc gttataagaa acaagaaaaa catttgaaag ctatttttca
     4801 atacttcaga tgaatcgttc ttattacaaa acaaactttt gtatcaccgg ttattcggaa
     4861 tttgagtaaa agtgtggggt aggactgatg tcccacctcc tttttttata aataaggaga
     4921 ttgtgtatgg aaaaaaacag gaagttcaac ctcgtcgcaa ctgcggcaag cgggctagaa
     4981 gcactggttg gtaaagaatt aagagatctt ggaatcgaat gccaagttga gaacggacgt
     5041 gcacgttttg aaggaacaat ggaaacgatc gctacagcta atttatggtt acgtacagca
     5101 gatcgagtaa aaatcgtggt tggggaattc gatgcgtata cgtttgatga attgtttgaa
     5161 aacgtaaaag cattgccttg ggaagatttt cttccactag atgcagcttt tcctgtagct
     5221 ggaaaatcga tcaagtcgaa actatatagc acacctgatt gccaagcgat cacaaaaaaa
     5281 gcaatcgttg aacgtctccg tacctattat caccgtccag cttctgtacc actgacggaa
     5341 actggcgcac attttcagtt ggaagtagct ttgctgaaag accatgtgat ggtaactctg
     5401 gatacgacag gaccaagttt attcaaacgt ggttatcgct tagaaaaagg tggagcacca
     5461 ttgaaagaaa acatggcagc agcacttatt atgttgacaa attggcgaaa agaccgtcca
     5521 ttttatgatc ctgtatgtgg ttcaggtacg ctttgtattg aagcagcctt aataggccat
     5581 aatatcgcac caggattcaa tcgtgagttt gcttgcgaat cttgggattg gttcagtcaa
     5641 gaaatcatgg aaaatgttcg atcagcagcc gaagaaaaag cagattatga tattgaatta
     5701 gatattactg gttcagatat caatggacgg atgattgaaa ttgccaaagc caatgcagaa
     5761 gaaatcggat taggctcttc gattaccttt aagcaacaag cagtcaaaga cttcaaaacg
     5821 gataaagaat acggggtcat cgtggctaat ccgccttacg gagaacgtct aggagaagaa
     5881 gaaactgttc ggaaattata taaagaaatg ggcgatgtat tccgtccatt aaaaacatgg
     5941 agtaaatata tcttgactag cgacttagcg tttgaggaat tttacggaca aaaagcaacc
     6001 aaaaaaagaa aattgtacaa tggtgcattg cgtacggatc tattccagta ttggggaact
     6061 cgtccgccta gaaaaaaaga gaaagacgaa taatcttact aattgattca ttcaattgga
     6121 tgtttggagg aatagggaat gaaagaaaaa gagtttttac aagaagtaaa agaaatcaat
     6181 cttctacaac aagctttagg aatcctcgat tgggatacac aaacaggtat gcctgaaaaa
     6241 gctagtgcat accgaagtga agtagacagc tatctttaca gcatttattt ttcaaaaaaa
     6301 atcggtccaa aaatcaaaga agccattcgc tatttttctg aacatccgga agaattgtcg
     6361 gaagttggaa ctgccgtttt tgccaaagta aaagaagagt atgatttgga acatcgcgta
     6421 ccggaagatc ttatgagaaa atatacagct gccgtttctt cagcacacgg acaatggcaa
     6481 aaagctagag aaacacaaca attttctgat tttcaacacg cattgaatca aaatattgaa
     6541 ttaacaaaac agttgatacc ttactggaga aaagatgaaa agacgaatta tgatgttttg
     6601 ctgaatcaat acgaacccgg gatgactgtc gaaatccttg ataaggtatt tgatcagctt
     6661 aaagaaggaa ttctttcgat ccgccgtacg ctccaagaaa aagggactga acctgaaaca
     6721 gactttttaa atcggagaat gacgaaaaaa caacagaaga gatttgtagt gaaagtaatc
     6781 gaacaacttg gttatgattt tagtcgtggt cgtttagatg acactgtcca cccatttatg
     6841 actggaatta atccgaatga tgcacgaatc acgacacgat ggaacgaaac agatttcaaa
     6901 atggctgttt ttggagtgat ccacgaggct ggtcatggta tgtatgaaca agacatcgat
     6961 gataaatatg cgtatacacc ggtttatgaa ggtacatcaa tgggtatcca tgaatcacaa
     7021 tcgttgttta acgagatcat tattggaagt agtcgcagct tctggaaaaa gcaatatcct
     7081 ttctttcaag aatgtgcaga aggaacattc gatgatattc cttttgacaa attttatcgt
     7141 tcattgaacc atacaaaatc aagtttggtt cgtatcgagg cagacagttt gacttaccct
     7201 ctccatatca tcattcgtta tgaaatcgaa aaaatgattt tcaatgaagg ggtagatgtt
     7261 gctgaactac ctgaaatatg gaatcaaaaa tatgaagagt atctgggaat acgaccaaca
     7321 aatgatgtgg aaggcatcct acaagacgtt cattggtcag gtgcaagctt tggttatttt
     7381 ccctcttatg cattagggta catgtatgcg gcacaattac ttcattcttt gcagcaagaa
     7441 atagatgtgg aaaatgttct agcgtcagac gattattctc caatcaaaaa ctggatgtca
     7501 gagcatatcc atcaatatgg tgcatctaga aaaccaaatc aattaatcat ggatgcaaca
     7561 catgaaccgc ttaatccgca atacctgatc gactatatga aagacctgta ttttaatgta
     7621 tatcaggtaa aataaaatac aatttacaaa gatagctgaa attcatctaa tctatagatg
     7681 ttttcagcta tttacttaag aaagaagtgg aacgaatgga taaattacaa gtgatcgctg
     7741 attatagtat ggaccaatta gcgcatgatc aaacaggaca tgggagcgat cataccaaaa
     7801 gagttgtcaa gttagctgaa aaaattttgg agacggaacc gcaggcagac cgctttgtga
     7861 cgcttgcagc agcttaccta catgatacga ttgatgataa agtcgttaaa aatgagaacg
     7921 aggcaaagca acagcttcgt gtattccttc gctctttgcc aatagtagag gaacagatct
     7981 cgatgatttt ttccattatc gaaaatatgt ccttttctaa aaatctctct gaagcggccg
     8041 aactatctct ggaaggtaag atcgttcaag acgctgatcg tatcgaagca ttaggagcaa
     8101 tcggcatttt gcgtactgct tatttcggcg gtggacatgg gcacccgatt tttgatcctg
     8161 aactgtatcc gcaaacattc aaggacaaag aaaattaccg aaaaggcacg acagtgatca
     8221 atcattttta tgaaaaactg ttcttgctgt ctgataaaat gaatactgat tacggatatg
     8281 aagaagccaa acgaagagaa accttcatgc gtgaattctt agaagaattt tttctggaat
     8341 ggtctattga cgatgaaaat tttactccag acaattggat caaaaataac aaaaaatgat
     8401 ggaggttgtg acagaagtgg tcagctccaa gatatttcgg atcattttcc gtaaaagtta
     8461 cttctggaac actgtttacc aagaggtcgt gagcctgccg ttcagctccg agtcacaagg
     8521 aacaatttca aaaaatagct tctcgtgttt gatgaatatt gtaacttgtg attcggaatt
     8581 ggcaggagaa caccgtttat tcggaagaat aggactgtga caagactttt gtcacagtcc
     8641 ttttttcgaa ttcaacaagt aaaactttta agaaatattc agagagtaaa attaaaaatt
     8701 gctgataaac gaagatggaa attttatgta tttttattgg tctatgcaat aaataaatta
     8761 aatattcaga atacaattga ttttaacgaa agggcttcta tcaatggttg tgttgtattt
     8821 atctgcccag gtagagtaaa atagatcatt atctaaaagg taattgttca tactatataa
     8881 gtcatttgaa agtaaaaaga tactgtaaat aaaaaatgtg gttaaataat tctcataatc
     8941 agtgtaagcg ttatcaaaaa tatagcatac ttgctttgta gatgaatgac acgatatatg
     9001 tttggtatgt tctgcaagac agatgaatat ggaggtggag acaatgtctt ccaaagaact
     9061 aacaatgtat ttagagaaag aattggatca actgaaagaa aaggggttat acaatacgat
     9121 cgacatacta gaaagtgaaa atggtgcttg tatcatcgta gacggaaaaa agatgatcaa
     9181 tctagcatcc aacaattatc ttggatttgc caatcgcgaa gaattgaaaa aagcatgtat
     9241 tgaagcaacc gaaacttatg gtgtaggagc aggagcagtc cgaacgatca atggttcgct
     9301 gaaaatccat cagcaattag aagaaaaaat cgcagaattc aaagggacag aagcagcaat
     9361 cgcctttcaa tctggattca actgcaatat gggcgctatc tctgcagtaa tgacaaaaga
     9421 agatgctatc ttatcggatg aattaaacca tgcatctatt attgacggtt gccgcttatc
     9481 cggagcaaaa atcatccgaa ttaaacatca agatatgaaa gaccttgaga aaaaagcaaa
     9541 ggaagcgata gaaagtaaga aatataaaaa aatcatgtat atcacagatg gtgttttctc
     9601 catggatgga gacattgcac gattgccgga aattattccg attgttgaaa aatatggact
     9661 gatcacctac gtcgatgatg cgcacggctc aggcgtaact ggaaaaggtg caggaaccgt
     9721 caagcatttt ggattaagcg acaaaatcga tatgcaaatg ggcacgttat cgaaagctat
     9781 aggtgttgtt gggggttatg tagctggatc gaaaacacta atcgactggt tgaaagctag
     9841 aagtcggcca ttcctatttt ctacttcgct aactccaggt gcagctgcag ctgcattggc
     9901 ttcgattact ttgatgcaag aacatccgga acttgtcgaa aaagtttggg aaaatgctaa
     9961 ttattttaaa gaagaattga aaaaagtagg ctataacatc ggtatgtctg aaacacctat
    10021 tactccagtc attttgggag acgaaaaagt gactcagact ttctctaaga agctgatcga
    10081 acatgggatt tatgctaaac caatcgttta tccaactgtg cctcttggga caggcaggat
    10141 cagaaatatg ccgactgcgg aacatacgag ggaaatgctt gatgaagcag tagcagttta
    10201 tcaaaaaata ggaaaagaaa tggaaattat ttaactaaat ttagtttttt cgtctattta
    10261 aaattagaga acatgttcac gcatatattg ttaatcatct gaagatagta gtaaaaaaga
    10321 tcagggagga tcatcatgaa aaaagtattg gtgacaggat gtttaggaca aattggttca
    10381 gagttgatca cacggctacg tcaagaacta ggcagtgaaa atgtcattgc gacagatata
    10441 cgcatgcctg aagtcattga gaaaaacgat atctttgaga tcctagatgt aatggattac
    10501 gaaaaaatgc gcagcatagc agaacaatat caagtggaca cattgattca tttggctgct
    10561 cttttgtcag ctgttgcaga agcaaaacca caattagcct ggaatttgaa cgtgactggg
    10621 ctggtcaatg cattggaagt cgctagagaa ttccagctga aattttttac gccaagttca
    10681 atcggcgcgt ttggtccaaa tactccaaaa gatcataccc cacaagatac tcttcagcgt
    10741 cctacaacca tgtatggggt aacgaaggta gctggagaac tactatgtga ttattattat
    10801 gtgaaatatg gcgtagatac tcgtggtgtc cgtttccctg gattgatttc ttatgcaacc
    10861 ttgccaggag gcggtacgac agactacgca gtcgatattt attattcagc aatcaaagaa
    10921 ggaaagtaca ctagtttcat tgatcaaggt acatcaatgg atatgatgta tatgccagat
    10981 gcgatcgatg ccattataaa actcatgaat gcagatcctg atcgattggt tcatcgaaat
    11041 gccttcaata tatcggcgat gtcttttgac ccggaagaga tcaaagcatc tattcaaaaa
    11101 atcataccag attttgaaat ggattatgat gtggatcctg tacgtcagtc aatagcagaa
    11161 tcttggccaa attcactaga tgtttcttgt gcgcaagagg aatggggctt ttcgccgaaa
    11221 tacgacttag atgtgatgac aaaggatatg ttggaaaaac tgaaagaaaa attggtagag
    11281 acggtataaa acaagaacca catccaaaaa gttttttata cttttgggat tggctatggt
    11341 atgaaagggg acaagggatg gaagaatatc aagccacgcc agtaaaagaa gtaaaaatag
    11401 aaaatgaatt aaaacgtacg atgggatttt ttactgcttt atcgactgtt atgggcacag
    11461 tgatcggtgc aggggtattt tttaaagccg caagcgtagc agaagttaca ggatccgcca
    11521 gtttgcatat gttttcttgg tttctcggtg gtatgatttc tgtctgtgca ggactaacag
    11581 gagctgaact ggcagctgct attccagaaa caggagggat gattaaatat atcgaacgga
    11641 tttacggaaa tacagcagct tttttattgg gctgggcgca agtcgtgatt tattttccag
    11701 caaatgtagc agccttgtcg atcattttcg gtacacagtt cgtcaatcta tttggtcttt
    11761 cccagtcaat gattgttccc gtagctgtta cagctgctgt atcgatcctg ttgatcaatt
    11821 ttttaggttc taaagctgga ggggcttttc agtcgattac gcttgtctgc aagcttattc
    11881 ctttgtttgt gatcgtcatt ttcggtttgt tccgacaaga aggagtagac ttccaactgt
    11941 ttccgatcca agcaggagaa aatctttcat ttttctcagc cttgggagcg ggcttgctag
    12001 caacgatgtt cgcatacgac ggttggatac acgtaggaaa tatttcggga gaattgaaaa
    12061 agccggcgaa agatttgcca aaagcaattt ctttaggaat catcggaatc atgatcgttt
    12121 atttattagt aaatgcggtg ttcttaagaa ccgcatctat cgacggagtc gcaggaaaca
    12181 gcaatgcggc aagtgacgtt gcaaaaatga tttttggcgg cttcggtgga agactggtca
    12241 cagttggtat cttgatttct gtttatggaa ccatcaatgg ctataccctt actggaatgc
    12301 gtctccctta tgttatggca aaagaaaaca acctgccttt tagcaagctc tttgctaaac
    12361 ttcatgataa aactaaagtt cctgtagccg caggtatctt agagctagtc atcgctattg
    12421 gcatgatgat gatcggtggt tttgatacac taacggatat gctgatcttt gtcatttgga
    12481 ttttttatac gatggtcttt gttggtgtca ttcttcttcg taaaaaagaa cctgatttgt
    12541 ttcgaccata caaagttcca atgtatccgt ttattccact agtagcaatc attggaggga
    12601 cttttatcgt atcaagcacg cttatcactc agaccttttt agcttcgatg gggatagtat
    12661 tgacacttgc tggtatcccg atttatctct atttaaaacg tagatagcta aaacattcga
    12721 attttattag aactgcactt ctctgccaaa caaaatattt tatttaaatg aggagtacgt
    12781 ggaagaaatg tcaaaagtac tcgttaattt tgatcacgta aatgtcgttt tgtctacgat
    12841 gaaatagcaa agtaaaagac agaaatcggg ctcaaccgat ttctgtcttt tactttgatt
    12901 tgcgtaactg aactattcgg cgtacgccgc gataaatatt taataaaacc aagcaaccag
    12961 aaatgaaaat gggaagccaa atcagataga cagggtagcc aagcacttta gacaaagcat
    13021 gtggagtcat tttatccatt tcaagaagtg cttgctgcca agtctgaaga ttgaagacta
    13081 gcatgaaacc cgaaataatc aggagaataa atcctaaata atgaattgta gttctttttc
    13141 ttacataagc gatcgtagca taaaggaaca gtaaaaaaat aggtacgcca aaaacaaagt
    13201 acatattcag catctcgaca ccccctttgt gtttatctta ctttactttg aaagaaatga
    13261 gaaataatag cctcgttact tgcttgaaac agcaaaggac tgctaagata agagagatac
    13321 aaagaatcaa ggaggagtca tatggcaagt tatatcgaac cgattcgttt tgcgctgatt
    13381 gtttttccat ttttagcctt agcgatctca tctgtttttt ttatttatga atatcgaaag
    13441 tatggaacat ttctagtaac aagggcagtt attttgtatt ctttcgtttt ttatttattg
    13501 tgtgcgtatt ttttggtgat cttaccactt ccggcaaagg agacggttgc ccaaatgaca
    13561 gggccaaaga tggagctgca tttatttgct tcatggcaac atttcagaga caacacggta
    13621 ttggtcttgt ctgaacctag tacctacctg ccggcaatga agcagagtgt atttttggaa
    13681 ccagctttta atgttttatt actcatacca tttggcattt atttaagata ttatttccgg
    13741 ctttctttta tgaaaacgat gattgctagt ttctgccttt ctctattttt tgaattgacg
    13801 cagctaagtg gactttattt tatctaccca agaccatacc gcttatttga tgtgaatgat
    13861 ttagtgacaa atacattggg tggaatcatt ggttttgtcg ttacgccgtt atttacattt
    13921 atgcttccta caagaagacg aatggatgaa ctttcttatg aaaaaggaaa gactgtctca
    13981 ttgactcgac gcttggtcgc ttatttgatc gactggacat ttttatctct tattaatttg
    14041 gtagcggcga ttttctttaa aattgcgttc gattcagggg cagttacttc tcatatctgg
    14101 tggcttttat tagaagtagg ggtatacttt atgattgtac cgtttataac caatggatgg
    14161 acacttggca aaaaaattgt gcgtatccgt ttggtggaag aagaccgcga acgaatttct
    14221 ttaaaagcat tgatgatccg ctacgggtat ctgtactttc ttttttatgg tttaagctgg
    14281 ttggggcagt tgcttcagca aatcatgaat tcgacaacga atacggtgtt gcagatgagt
    14341 gcagtattca tttctctcgg tgtcttgtta ctacaaggta tttttatttt aaatatcttg
    14401 ctatcatttt tgagaaaaaa acggattctg ttttatgaaa aagctagtca cacatatact
    14461 gttagcacga ttggccaaac aaaggatgat gattctcgaa attagctgag caaaaacgct
    14521 ttgtctgtgt tataatgcca aagtcgtgat aatttaaaga gaagaggtgc acgaatgaat
    14581 aattttgata tattccgctt caaaccgttt atcaatgaag cgttaaccgc aaagggcttc
    14641 aaacagccca cagaggtcca ggaacgtttg attcctttaa ttgcaaaagg aaaaaatatc
    14701 attgggcaat ctcaaactgg tacaggaaaa acacatacgt ttttgttgcc tttgtttgaa
    14761 aaaatcaatg cagaaaaaga ggaagtacaa gttgttatta cagctccaag tagggaacta
    14821 gctgcacaga tctatgaagc agctacacaa atcgctaaat tttctacacc ggagatccgt
    14881 gtctcgaatt ttgtcggcgg tacagacaaa cagcgtcaat taaatcgtct gcatcaccaa
    14941 cagccacagg ttgtagtagg cacgcctggt agaatattag acatgatgaa tgaacaagcg
    15001 ctgaagattc acaccgcatt tgcatttgtt gtcgatgaag cggatatgac attggatatg
    15061 ggttttttaa atgaagtaga ccaaattgca agtcgattac cagaaaagct gcagttttta
    15121 gttttttctg ccactattcc agaaaaactt cgtccattct tacgaaaata catggaaaat
    15181 ccaatcattg aagaaatcaa accaaaagca gttatttctg aagacattga aaattggctg
    15241 atttcaacaa aaggtaaaga tgagaatcaa atgatttatc aactgctgac aattggtcat
    15301 ccttatttag caatcgtatt tgccaataca aaacagcatg tggatgaaat tgccgattac
    15361 ttgaaaaacc aaggcttgaa agtagcaaag attcatggag acatcacccc aagagagaga
    15421 aaacgagtga tgcgacaagt acagaatctg gagtatcaat atgttgtagc gactgattta
    15481 gctgctagag ggatcgatat cgaaggtgtc tctcatgtta ttaatgcaga aattccagaa
    15541 gatctagact tctttatcca tcgtgtcgga cgtactggac gtaatggctt aaaaggaacg
    15601 gccattacgt tatattcgcc aaatgatgag caagccattg atgcgataga aaaattgggc
    15661 gtctcatttg aaccaaaaga aatcaaaaac ggggaaatcg tcaaaacata cgatcgtaat
    15721 agacgtgcca aacgagaaaa atcaaaagat gtattagatc ccacgctgat tggtcttgtg
    15781 aagaagaaaa agaaaaagat caagcctggt tataagaaaa aaatcgattg ggcaattgca
    15841 gaaaaaaata aaaaagaacg caaaatcgaa cgcagacagc aaagccgtac tgctcgtaaa
    15901 aataagaaaa attcacataa ataaaaaaca agaggttgtg acaaaaggcg tgtcacaatc
    15961 tcttgttttt aagctagact ttttttcatt ccgatacaaa gtacttcatc ttcaataaaa
    16021 ggttctgtgc aagtatgata cccttgtttt tcataaaaat ctacagccgt catttcagct
    16081 actacataac tagtctgaca accttctgaa accgcccgtt tttcagcagc ttcaagcaac
    16141 tgaccgccta acccttttcc tcggtattct tttgctacgc aaaatcgttc cggcttgaag
    16201 caagtaggtg ttttcttttc ataacgcaaa gtagctactg gtcttttttg atcaagaaat
    16261 agataatagg tttgttgcgg gatatctttt ccgtcgaatt ctagttctgc aggaatccct
    16321 tgttctaata caaaaacttg atatctcaaa aagcagctag ctgcgcgtat ccattcatcc
    16381 gctccaacta tttttttcat catattcctc cttgtcatga atagcagaga tgtattgatt
    16441 cctttattct tatcgtacca aaaatcgtgg agtatgacca aaaggaaatg aaaataaaaa
    16501 ttgagatatt gacagtgatc aatagaatgc gtatactaat taagtacgaa aaggatatga
    16561 tttgtttttt aagactcaca gaaaattctt tcttggctga aaaaagaata gtcactaatc
    16621 aaattgctcc ctttttctat tttggtagaa ataccgacgc agctcagcgt tatcgagttt
    16681 aagaggtaat gattacacat cattgcaatc aaggtggtac cgcgagtttt cgtccttggc
    16741 tgtgatggtg tttttttgta tttaatttat agaaagcagg taatgacatg aaacaattaa
    16801 gtagtgcaca agttcgacaa atgtttcttg atttcttcca atctaaagga catacgattg
    16861 aacctagtgc gtctttgatc cccgtgaacg atccaacctt attgtggatc aactctggtg
    16921 ttgcaacatt gaaaaaatat tttgatggtt cagttgtgcc agaaaatcca agaatcacaa
    16981 atgcccaaaa atctattcga acaaatgata tcgaaaatgt cggtaaaaca gcacggcacc
    17041 atacgatgtt tgagatgctg ggtaattttt cgattggtga ctatttcaaa aatgaagcca
    17101 tccactgggc atgggaattt ttgactgatc caaaatggat ggcattcgat ccagaaaaac
    17161 tttatgtaac agtttatcca aaagatacag aagcaaaacg catctggcat gaagaagttg
    17221 gcctgccact agatcacatt attgatgtag aagacaactt ctgggatatc ggtgcaggcc
    17281 catgtggtcc ggatacggaa attttctatg accgtggaga agcttataac gatttagcag
    17341 aagacgatcc tgaaaactat ccaggaggag aaaatgaacg ttatttagaa atctggaact
    17401 tagtattttc agaattcaac cataagcctg ataacacatt tgaaccactt cctcataaaa
    17461 acattgatac ggggatggga ttggaacgca tggtctcaat cgttcaagat gcgtcaacga
    17521 actttgagac agacttgttt atgcctatta tccacgccgt agaaaagcta agcaaacaag
    17581 taaaatatgg tgaaaaccca gtaacagata tttcttacaa agtaatcgca gatcatattc
    17641 gcgccctttc atttgctatt ggcgacggtg cattgccatc gaatgaaggg agagggtatg
    17701 tcttacgccg tttgcttcgt cgtgctgtca tgcatgggaa aaaattaggt attgaacaag
    17761 cattcttata tcgtttagtc cctgttgttg gggaaatcat ggtaagttat tatcctgaag
    17821 tacttcaaca aaaagaattt attgaaaaag ttatccgtac agaagaagaa cgtttcaatg
    17881 aaacaatcaa tgaaggtttg gaaatcttaa atcaagtgat ccaagaagta aaaaacgaaa
    17941 acgggacgac tctagcagga aaagacatct tcaaactata cgacacatat ggcttcccgg
    18001 tagaattaac ggaagaggtg gcagaagatg aaggactgaa agttgatcat gctggttttg
    18061 aaaaagaaat ggatgcacaa cgcgaacgtg ctcgctctgc ccgcagtact gaaaaatcaa
    18121 tgggggtaca atctgcttta ttgacagata tcaaagtaga aagtaaattc attggataca
    18181 cagaacaaga atcagatagt caattattgg tcatcgttaa aggtgagtct cttttgaatg
    18241 aagtcgcaga aggtgaagct caaatgatct ttgatcaaac acctttctat gcagaaatgg
    18301 gtgggcaagt agcggatcaa ggatggatca tgaatgaagt aggcgaaatc gtagcagttg
    18361 ttacagatgt tcaaaaagcg cctaatggtc aatttatgca caccgtagaa gtaaaagcac
    18421 caatgagtga aggacaaacc tatcatctat tgatcgatca atcgatgcgc aatcggatca
    18481 tcaaaaacca tacagcaacc catctactgc acaaggcatt gaaagaagta cttggcgagc
    18541 atgcaaatca agctggttct ttagtcgctc ctggacattt acgattcgac tttacccact
    18601 ttggtcaaat cacttcagat gaattagctg aaatggaaag aatcgtcaac gaaaaaatct
    18661 gggaagctat cccagttgaa acaattgaaa cagatatcga tacagccaaa aacatgggtg
    18721 caatggcttt gtttggtgaa aaatacggac atgacgttcg agtagtaaat attggcggct
    18781 ggtcaatcga actgtgcgga ggcacacatg tagcaaatac agaaaacatc ggaatcttca
    18841 aaatcgtttc tgaatcagga attggtgcag gcgtgcgacg aatcgaggct gtaacaagta
    18901 aagaagcgta cgaactactt gaagaagaag aaaaacaact aaaacaagta gcaacaattg
    18961 ttaaatcacc tcaattgaaa gaagttgttt caaaagcgga acaattgcaa cagcaagtaa
    19021 gagagctaca aaaggaaaat gaacaattag ctagcaagtt agcgaatcaa caagctggtg
    19081 atgtattcaa agatccagaa acgatcaatg gaaaaacaat cattgcagca caagtaaaag
    19141 tgaaagatat gaatcagctg cgtcaattag ctgaccaatg gaaacaaaaa aatatttcag
    19201 atattcttgt tttagcaaac gaacaagaag gaaaagtcag tctgctagcg gcaatgacga
    19261 aagaatcaaa tgaagctgga ctaaaagctg gcgacttgat taaagcaatc gcaccgaaag
    19321 taggcggtgg cggtggtgga cgtccagaca tggcacaagc tggcgggaaa aatcctgctg
    19381 gtattccaga tgcacttaaa gctgcgaaag aatgggttgc gcaacaatag ggcgtttacg
    19441 tttgacagaa aaacaaaagt tcaaacattg aacgaaacat gattatgggg gcgaagacaa
    19501 acaagtcttg ccctcatact tttttcaatt ggatggcgca tctaatcaaa tggtcggatt
    19561 cggatggtct ttagttgacg aaagtgacag aaatttttac gattagtata aataaaaagg
    19621 ggtgaaaaaa tgaatagtag aaataagacc tttcgactag tcttacgtgc gatcttgtta
    19681 gcaatcatca tcgtacaagc gatggttcct tggctggggt ttattccatt aggattcatt
    19741 agtttaacaa ttatccatat cacagtaatt attgctgcag tagtattagg cccaaaagat
    19801 ggcatggtga ttggcttgtt ttggggaatc gctacaattg ttcgtgccta tgccatgcca
    19861 acaacgccat ttgatacatt agtgttcacg aatccgatta tctcggtagt tcccagagta
    19921 cttgttggct tagtagcagg cttggttttc cactggattt atcaaaaaaa taaatcaata
    19981 acgatcagct cagcctttgc aggggtactg ggttcattgg tcaatacagt cttagtgtta
    20041 ggattcatgg gattatttta tacaggtgca acggcagatg cctatggtgt agactctgct
    20101 cttttattta aaaccttagc aggggttgca gcaatcaatg ggataccaga agcaataggt
    20161 gcgggaatca taacaccatt actagcaaaa gcgttatttg cagcaacacc gttgaaacca
    20221 gaataatgac catcttttaa tagtaatagg acgcacgata gatagaaaaa ggaagagagg
    20281 agaggaaaac aatcgtcctc tccatctttt tacctgagca acaagcaaac tagaaggggt
    20341 aaaaaacttg gctaagaaac aagaagaaaa aaagaaacaa ataccgcaac agcggacaaa
    20401 aaaaggagta ctagcgggta tcttaggatt ggttacgaat ctgatcttgt ttgtctctaa
    20461 gctaatgatc gggctttttt caggaagtgt ctccattatg gctgatgcag tgaacagtct
    20521 atcagatact gcttcatcca tattgacttt gatcggattt aaaattgctg caaagccagc
    20581 agatcaggaa catccttatg gacacgaacg cttcgaatat atcagtgggt tatttgtttc
    20641 gatcatcatt acttatgtag gatttcaatt tttagagtct tctgctgaaa aaatcatcca
    20701 tccagaaaat gtctctttaa cacccatcgt cttttttgtc ctaatcattt ccatcttttt
    20761 gaaatttgca caaggcaaaa tgtatcaggt tattgcgcaa tccattgatt ctgaaacatt
    20821 gagagctaca gcaacagata gttataatga tgtctttact acactggctg tattgctttc
    20881 agcagcaatc gaatgggtaa ctggatggcg tattgatgga tatataggtt ttgctttagc
    20941 attatatatc ctttatagtg gattgacgat gcttcgaaat tttgtatacg aactattagg
    21001 aagtcgccca acgccagcag agattgaaga aatggaagag catttaaatg cttgcgatga
    21061 tatacttggc tatcatgatt tattggtgca taactatgga cccaataaac gctttgcatc
    21121 agtccacatc gaagtagatg aatcaatgaa tttgaatgtg gctcataaga ttattgatct
    21181 gatagaaaaa gactttaaaa agtctttgga tgtggaactt gtctgtcact tggatccaat
    21241 gcctgttgat aacgaaaaat actataatat cttgcggcag ttaaagcaaa ttcttcttga
    21301 aacaggtaca gggcttaata ttcatgattt tagagtaatt catcaaggaa aaacgctgca
    21361 atttgatgta gtggtccctg agaaattcca gtttgttgat acagaactag aaagaatctt
    21421 acgtagtcaa atagaagaaa aaatcggaga ttaccaattg gaaattacgt ttgatcacaa
    21481 ttatctttta tattaaatga gaaactataa aaagactcct taaagttttt aatttaaaga
    21541 gtcttttttt cttttaattg tttatggaga gcctgacgtg caagattatc tgctccttta
    21601 tttttgtttt cgggcaacca ttgcaggatc aataaagaaa attcggatag aagttcttgg
    21661 attctttgaa ggtagggaag gaagagctcg ttcttcgttg cgtttttgtc gaccgtagaa
    21721 actagtgttt tgctgtctga aaaacagaaa atcgtttcat tctgtaagtc gtttttcttt
    21781 agaaattcta aagtttttaa aaagacttcg aactctgctt gatggtttgt ccctatagaa
    21841 agcgggatag atagctgttc ttgtttacct tgatagacaa tcaagattcc acctccacta
    21901 ggaccagggt tgccttttgt agaggcatca atgtatactt ttaacataaa aagagattct
    21961 cctttttgtt caaactaatg ttatggtaag attatatcat tactgaatcg gtgagaggtt
    22021 agagaatgaa aaaaaatatt cgatggcagc ctgaactggc acatacgatc atctattggt
    22081 cattgacctt gatcattctg ttttttagtc tgattctttc tttagaaaac acaggtcctt
    22141 attggaaaag caattttgtt ttaggtattt ttcttgtttt tctatttcta ggtttgcggc
    22201 gttcttttat tttaaaatca aactctgtga ttatacgtta tgctgctttt tggaaagacc
    22261 gagaaatatc tgtcggacag attcgatctt tgacgccaac taatactggg gtgcaaatga
    22321 tgttgcaaga tgagaaagcc ccatctgttt tcatgatgcg aaaaaaagat caagtagtcc
    22381 tttttgatca ttttgaaaaa aacggaccca aatcagaatc aattccttat ccttatacga
    22441 aaaaaagaaa aagctaattt taaaaatcag aacctaaaaa aacgaatttt tacaggaaaa
    22501 gaaaagagtg agacgtacgg ttcatcccgt ttagcgtctc actctatttg tttatctatt
    22561 catgcatact agtatcggta gtctgaacgt tgcctttctt gacatgggca gcttgtgttc
    22621 ctcgattccc ttcgatgatc tcaaattcga ccacttgatt ttcttccaaa gttttgaatc
    22681 cctcttcttc aatcgcagta aaatgtacaa aaatttcttc tgtgtcatca taactgataa
    22741 agccgtatcc ttttttgttg tcaaaccatt ttacaattcc tgtggtcaaa gcactcacga
    22801 cctttctaaa gtctgatcat agccttcatt ataaagagta tttataagta cgtcaaataa
    22861 attaaaaacc tgtaatttta tgtttttttt ctgaataaaa aaaggtataa ttggcatatc
    22921 ttaacaaaga agatattttg ttgaataaag ttcgtgaaaa ggggtaatca aatgaagaat
    22981 gatctgcaaa tttcacaaga aacagagcta gaaccgattg ttgaagtggc tagtaaatta
    23041 gatcttaagg aagacgattt ggaactgtat gggaaatata aggcgaaaat caatttttct
    23101 ggaatcaatc gatcaaagga taacgctgag gggcatttga tcttagttac ttccatcaat
    23161 ccaacaccag ctggcgaagg aaagtcaacg atcactgtag ggttaggaga cgcgctcaat
    23221 aaaatcaata aaaaatctgt tattgcattg cgagaaccct cacttggtcc tgtgatggga
    23281 atcaaaggtg gtgctgcagg cggaggatat gcacaggtat tgcctatgga agatatcaac
    23341 ttgcatttta ctggtgacat gcatgctatc acaacagcta ataatgcatt atctgctctt
    23401 ttagataatc atattcatca aggaaatgaa ttgaacatcg atgcccgccg agtgatttgg
    23461 aaacgggtag tcgatctcaa tgatcgtgaa ctgcgtaaag taattgttgg cttgggtggg
    23521 ccaatccaag gtgttccaag agaagatggg tttgatatca cagttgccag cgaaatcatg
    23581 gctatcttgt gtcttgcggc agaccttgaa gatttaaagg ctcgtcttgc aaggatcgtt
    23641 gtcggctata catttgaccg tcagcctgtg actgctggag acttgaaagc agaaggcgct
    23701 ctggcattac tgctgaaaga tgcaatcaag ccgaatcttg tacaaacaat ttacggaaca
    23761 ccagcttttg tccacggggg accgtttgcc aatatcgctc atggctgcaa tagtatttta
    23821 gctaccaaga cagcattgag attagcagat tacgttgtca cagaggcagg atttggtgca
    23881 gatcttggcg gagagaaatt tttagatatc aaagtaccta acttgaaaaa agcgccggat
    23941 gctgtggtga ttgtcgccac tgtccgtgca ttgaaaatgc atggcggaat gaaaaaagat
    24001 gaacttaaaa atgagaatct tgatgctttg aaagtaggtt ttgcaaattt aaagcgccat
    24061 atacgtaata tggaacaata tcagctacct gtgatcgtcg caatcaatga attcgtaaca
    24121 gatacagaca atgaactcac actgttggag cacttgtgtg aaaatcaggg gattcttgcc
    24181 aagagagcga gtgtatgggc agatggagca gaaggcggcg ttgatttagc aaaagctgta
    24241 gtacgattga ttgatagaaa agaagctaat tacaagccat tataccgcct agaagaaacg
    24301 atacaagaaa agacagaaac aatcgtcaag aaaatttacg gaggaaatgg tgttgttttc
    24361 agcaaaaaag cgcaaaaaca aatagaagag tttacgaaga atggatggga caaactgccg
    24421 atttgtatgg caaaaacaca gtattcattt tctgatgatc cttcactgct aggtgcaccg
    24481 gaagatttca ccatcacgat ccgcgaaatc atcccaaaat taggggcagg atttttagta
    24541 gccttgactg gagatgttat gacgatgcct ggtttgccta aaaaacctgc tgcattgaat
    24601 atggacgtga caaatgacgg agaagtgttg ggattgtttt aagcttgatt ataatctacc
    24661 atcgaaggag ctgtattttg actacagctt ctttttttag acgataggga attaattttg
    24721 tttacactag gatcattctt tttctttatt gtattttgag ctatactaaa gcataatagg
    24781 agggtatgat gaaaacgaca tttataagaa aaaccggttt ttacggtatg ggtagctcga
    24841 ttcagctaag aatcaacaat gaaaaacgca gtgttctcag tcatgaacaa aagataacgc
    24901 ttgatctgga agtgcccttt acgatgcagg tcacctttta ttggctaaaa agccccgttt
    24961 atacagtgaa agaagcaaag cctgcttatg tgatcacaat gaattacttg attcttcaaa
    25021 tctatccgtt tttatttcta gcaactgggt tgggggccgt tttcctacaa aatttatttt
    25081 tcagtttttt tgcagttatt gtgttgattg cgttcttttt ctatataaaa aaccgggcat
    25141 atttgatcaa ggaggaaaat tatggcaaac tctgatcttt ttttaatcag tttcaatcga
    25201 atcgaaaaat ggatgagaga cgagatggga aatgcacgaa atatgggatt taccgaactt
    25261 gtcagacgcc tagctcaaag aaaacaatta atgatcagaa aatacgaaga tgatctttta
    25321 cagcttgctc agttaaggaa tgcgatcgtc catgatcgga tcgcagtcga ttttgtcatt
    25381 gcagagccaa atgattggac agtcaagcga atccagctga tcgaacaaga actgattcag
    25441 cctgaaaccg tgctgcctcg atttgcaaaa cgtgtaacag gttttgaaca agatcttccg
    25501 ttacttgaac tgttaaagat agtagcagaa aaaaggtatt cacaatttcc gttatacaat
    25561 aaaggaaaat ttgtagcatt gattacttta cgtaatatcg ggttttggct agcaaaagaa
    25621 agccaaaaag gtccggttga tttaacaaat aaacgagcgt tggatttgat catccaaaac
    25681 ggaaagtaca caaattatca ttttgttcca gctaaaacac atatttacga ggttgaagcg
    25741 atgtttcgtg agcaagggac tttggaagcc gtacttatca ctaaagatgg gaatcccgat
    25801 ggtaatctgc tcgggatcgt acgacctcgt gatatttata aacaaataga aaaggattga
    25861 ttttttgtta attatttatt ggatattcag cgctttgcta gtaggagtgg atcagttggt
    25921 caaatggtgg atcactgata atctagcgct tggagagacg aaaaatctta taccaggtat
    25981 tttgtcactg aatcatattc gaaacactgg agccgcatgg agtatgctag aagggaagat
    26041 gtggttcttt actatagtga cactcattgc agtcgttgtg attctcacat tgatgattaa
    26101 aaatcgtgaa aaaggtaatc gctggttcat gattggattg agtttgattc tagctggtgc
    26161 gattggcaat tttatcgacc gtattcgtct tggctatgta gtagatatgt tccagacaga
    26221 ttttatgagc tttccgattt ttaacgtagc agatgtgaca cttgtatgtg gagtgatttg
    26281 tatgttaatt tatattattt tagatgaaaa ggatcaaaga aaaaaatgac agaacttaaa
    26341 acgacgattc accatgaatc tggtaggatc gataaagtat tgactaattt attcaccgat
    26401 tacagtcgtt cgcaaattca gctgtggctg aaaaatggag cggtttctgt caatggacag
    26461 ccagtcaaag caaattacaa agtaaaacaa ggagacgaga ttactgtctt gatccccgaa
    26521 ccagaaacat tagatgtggt cccagaaaat atcccgttga cgatcgttta cgaagatgaa
    26581 gatgtagcgg tgataaacaa acctcaagga atggtcgttc atccttcagc aggtcatacg
    26641 catggcacaa tggtcaatgc gctgatgtac catatcaagg atctttctac gatcaacggc
    26701 gttattcgtc caggtattgt tcaccgaatc gacaaagata cttccggact gctaatggtt
    26761 gccaaaaatg accaggcaca cgaatcatta gctcagcaat taaaagataa gacctctttg
    26821 cgcaaatatg ttgctttagt ccatggagag attcctcacg aaaaaggaac catcaatgcg
    26881 ccaatcggcc gttcgaagga aaaccggaaa atgcaggcga taagagaaga tgggaagtcg
    26941 gctgtcacac attttactgt attggaacgt ttcgaaggat ttactctggt tgaattaaaa
    27001 ctagaaaccg gaagaacaca ccagatccgt gtgcatatga agtatatcgg ttatccgtta
    27061 gctggagatc cagtatacgg accgaagaaa actttgaaag gcaatggtca gtttctccat
    27121 gcgcaaatgt taggttttac gcacccgact acaaacgaaa agatgatctt tgaagcacct
    27181 cttccagaag tttttgaaaa aactttagaa aaattaagaa aaaacattgc attttaattt
    27241 agataacggt atagtaatta acaacgaaag cataaaactt aatagaattc ctttaagttt
    27301 agtcccgtga ggctaagaag ggtgggagca aggatacgta tggtgcgcca taagtagatt
    27361 gctcattgac atagtatagc tatcagtgta tcagtatacg ctcagtctaa acattcctcc
    27421 tgtcctctta gggcaggagt ttttttatgc aaaaaagtag ggggaatgaa aatgcaagca
    27481 aaagaagtag tagatcaggt aaccatgaag cgagcattga ctcgaattac ttatgagatt
    27541 atcgagagaa accatagtat ccaagatatc gtattggtag gtatcaaaac acgaggaatc
    27601 tacattgcat caagaattgc cgaacgcctg aagcagctag aagatatcga tattcctgta
    27661 ggagaactgg atatcacttt ataccgagat gacaaaaaag aaaatccaga agaaccggaa
    27721 cttcattctt ctgacatccc agtttcctta gaaggaaaag aagttatttt gattgatgat
    27781 gttttgtata caggacgaac gattcgagca gcaatggacg cagtaatgga ttttggcaga
    27841 ccaagaaaaa tttcattagc agtcctggtc gatcgtgggc atcgagaatt gccaatccgt
    27901 gcagattatg ttgggaagaa cattccaaca tctcgggcgg aagagatttt agtagagatg
    27961 caagagcttg atggacaaga tcgcatcatg attttgaaag aggaagatta gaggagagag
    28021 aaacatgtcg gagaaaaaag gatttcataa tgatgacgtg gtgttagata tccacgatcg
    28081 cccaacccca ggacattggg taggattaag tttacaacac ttgttcacga tgttcggagc
    28141 aaccgtgtta gtccccatct tagtaggtat cgatccaggt attgcgctag tcagttcagg
    28201 actaggtaca ttggtgtact tatttactac taaaggaaag atacctgcgt acttaggaag
    28261 cagcttcgct ttcattgcag cgatgcagct tttgatgaaa acagatggtt atccagcgat
    28321 tgcacaagga gcagtcacaa ctggattagt ctatttgatt gttgcgctga tcatcaaaaa
    28381 gatcggttca gcatggctgg ataaaatctt gcctccaatc gtcgtcggtc cagtcatcat
    28441 ggttatcggg ttgggacttg cggcaaatgc agcaacaaat gctatgtata atcagggcac
    28501 gtatgatttc aagtatattg ctgtagcgct gatcacatta ggattaacaa ttttcttcaa
    28561 catgtggcta agaggttttc tcggattgat tccgatttta ctaggtatta ttagcggata
    28621 catcgtagcc ttgctgtttg gaatcgtgga tactaagcca atcgtagacg ctgcctggtt
    28681 tgctgttcct aactttgaga ttccctttgt acaatatgaa ccaaagctat atcttggagc
    28741 aatcacaaca atggcaccca tcgcttttgt gacgatgaca gagcatatcg gccatcttat
    28801 ggtattgaat aaactaacta aacgtaattt ctttgaagaa ccgggtcttc ataagacgtt
    28861 gacaggtgat ggattagcac aaatcgtggc agcctttgtc ggaggtcctc ctgtaacaag
    28921 ttacggtgaa aacattggtg ttctggctat cacacgtgtc catagcgtgt ttgtgatcgg
    28981 tggagctgca gtatttgcag tcattttagg attcgtagga aaattaagcg ctttgatttt
    29041 aagtatccct ggaccagtca tctcaggaat cagttttatc ttatttggag taattgccgc
    29101 aagtggaatg aagattttaa tcgagaacaa aatcgatttt gataaaaaga aaaatctgtt
    29161 gattgcttca gttatcttag tagtaggaat tggcggactc gtctttgaag taggcacatt
    29221 tacattgtcg gcaatggcat tagcaactgt acttggcatc attttaaatc ttatcttacc
    29281 agaaacttca cgaagcgaag aaaactaagg aggaaacatt atgatcatca aatcagaacg
    29341 tatcagctta aagcatttat tgaccgttga ggcacttact gatcaagaag ttatgggctt
    29401 gatccgcaga gggcaggaat tcaagcaagg tgctaattgg actccgcaaa agcgccagta
    29461 ctttgctaca aacctctttt tcgaaaatag tactcgcaca cataaaagct tcgatgtagc
    29521 ggagaaaaaa ctagggctag aagtgattga atttgaagca agcacaagct ctgtccaaaa
    29581 aggggaaaca ctatacgata cagtactgac gatgtcagca ttaggcgtag acgtagcggt
    29641 tatccgtcat ggtgatgaga actattatga cgaactgata caaagtaaaa caatccagtg
    29701 ttcgattatc aatggaggag acggaagcgg acagcatcca acccaatgct tgcttgattt
    29761 gatgaccatt tatgaagaat ttggtcattt tgaaggtctg aaagttgcca tcgtaggtga
    29821 tatcactcac tctcgcgtag ctaaatccaa tatgcagatg ttaaaacgtc taggcgcaca
    29881 agtattcttc tcaggtccta gagaatggta cgacgatgaa ttcgaagtgt acggacatta
    29941 tatgccattg gatgaactac tagatcaagt tgatgtcatg atgatgcttc gggtgcaaca
    30001 cgaacgccat gatggaaaag aaagtttctc aaaagaaggc taccatcagg aacatgggtt
    30061 aacagttgag cgtgcgaaga agttgcaaaa acatgcaatc attatgcacc cagcaccagt
    30121 taaccgtgat gtagaattag ctgattcatt agtggaagga ttacagtcgc ggatcgttca
    30181 gcaaatgagc aatggagtat tcgtcagaat ggcaatctta gaagcagtgc taaatggaaa
    30241 agcgtaatta taggaggatg aaaaaatgaa aacactcatt aaaaacggtc agatcaatac
    30301 acgaaaaaat atgacgacac cagcagaaat ttggatagaa gatgggcgaa tcaaagcaat
    30361 cggtacagga ttctcagaag ccgaatttga tgaagtgttt gatgcaaagg gacaattgat
    30421 cacacctggt ttagtggacg tccatgttca tttgagagag ccaggattta catacaagga
    30481 gacgatcgaa gctgggactc gctcagctgc tcgtggcggt ttcactactg tatgcgcgat
    30541 gccgaattta aatcctgtac ctgatacggc agaaaagctg agacaagtat atgacatcat
    30601 tagaaaagat gcagttgtga aggtattgca gtatgcgcca atcacagaaa atctgcgaag
    30661 tgagaaatta gttgatcaag aagcactgat tgaagaaggt gcttttgctt tcacaaatga
    30721 cggtgtaggt gtccaaacag ctgggacgat gtatctggct atgaaagaag ctgcaaaaaa
    30781 caataaagcc ctagtagccc atacagaaga tgaatcttta ttatttggcg gcgttatgca
    30841 cgctggcaag aaggcagaag aacttggtct tcccgggatc ttaagcgtaa ccgagtcttc
    30901 tcaaattgct agagatctgt tgctagctga agcaactggt gttcattacc atgtttgcca
    30961 tgtttctact aaagaaagtg tccgtgtaat cagagacgca aaaaaagcag gaatccacgt
    31021 aacagcagaa gtttcacccc atcatcttat tttgatcgac gaagatattc ccgaagattt
    31081 cggcttttgg aaaatgaacc cgccgctaag aggacgggag gacagagaag ctttgatcga
    31141 aggcttgctt gacggtacga tcgattgcat cgctacagac catgcccccc atggattaga
    31201 agaaaaaagc caaagtttca tgaaatcacc atttggtatc gtaggaagcg aaacagcgtt
    31261 tcaactgatc tatactcatt ttgtagaaac tggtcgattc acgttggaac aagtgatcaa
    31321 ttggttggca gttaaaccgg ctgagatttt tggtttgaat gcaggaacgc taacggttgg
    31381 tgcccccgct gatgtagcag tttttgatat tacacaaact tgcacgatcg ataaagaaga
    31441 ctttttatca aaaggggaga atacaccttt tatcggctgg aaagtaaaag gcgaaacaca
    31501 aatgactttt gtcaacggaa aacttgtgtg gcagaaggga gaataaaagt atggaacgtt
    31561 tgcttatatt agaagatgga acagttttcg aaggaaaggc ttttggtgca gatgctagtg
    31621 tcttcggaga aatcgttttt acaacaagta tgactggtta tcaagaaact atcactgatc
    31681 aaagttttaa cggtcaaatc attacattta cctatcctat ggtgggtaat tatggggtaa
    31741 atagagatga ttatgaatcg atttctccaa catgcaaagg agtagtagta aaagaacatg
    31801 cacgtgtagc ttcaaactgg cgaaaccaaa tgacgctaga ttcatttttg aaacaaaaag
    31861 gaattcctgg aatatcaggt atcgatacgc gcgcccttac tcgcaaaatc cgtgaagtcg
    31921 gaacgatgaa agctagtata gttgatgctg gagattcctt cgaacatgca tttgatcagc
    31981 tgaaagcaac cgttctagca accaatcaag tgcagcaagt ctcaactaac aaaccttatc
    32041 caagtccagg aacaggtcgg aaagtcattg tcattgactt cggcttgaaa catagtattt
    32101 tacgagaatt gagtaaaaga aactgtaacc tcacagtact tccctataac acagatgccg
    32161 agacgatttt agcgctatca ccagacggag tcatgctgac gaatggacct ggtgatccaa
    32221 aagatgtgcc agaagcaatc gaaatgatca aaaaaatcca aggaaaagta ccgatttttg
    32281 gtatttgtct cggtcaccag ttatttgctt tagccaacgg tgcagatacc tataaaatga
    32341 aattcggaca tcgaggatta aatcacccgg tacgagaagt agcgaccgga agaattgatt
    32401 ttacttctca aaatcatggc tatgcagtcg atgaagccac gattgatcca gaaaaactca
    32461 tcgtaactca tgtagaagta aatgacggaa cagtagaagg agtcaaacac agagattatc
    32521 ccgctttcag tgtgcagttt catccagatg ctgcccctgg tccgcatgat gcactacatt
    32581 tatttgatga atttatggaa atgatggacg caggaaagga gcagtaaaca gatgccgaaa
    32641 cgtacagata tcaagaaaat tatggtgatt ggatctggtc cgatcatcat tggtcaagcc
    32701 gcagaatttg actacgcagg aacacaagcc tgccttgcct taaaagaaga aggatatgaa
    32761 gtggttttag taaattctaa tcctgcgacg atcatgacgg ataaagaagt ggcagatcaa
    32821 gtatatatcg aaccgatcac acttgaattt gtatcaagga ttcttcgaaa agagcgtcca
    32881 gatgctctcc ttccaacttt aggcggacag acaggcctca atatggcaat ggagctggca
    32941 gcatcaggta ttttagaaga attgaacata gaacttctag gaacaaagct ttctgcgatc
    33001 gatcaagcag aagatcgtga tctattcaaa cagttgatga aagaattgaa tcagccaatc
    33061 cccgagtctg aaatcgtcaa tacagtagaa gaagctgtag attttgcaaa taaaatcggc
    33121 tatcctgtca tcgttcgtcc agcctttact ttaggcggaa ctggtggagg tatgtgtgaa
    33181 aatgaagaag aattacgtat cgtatcagaa aatggattaa gtctttcccc cgctacccaa
    33241 tgtttgattg aacgaagtat tgcaggtttt aaagaaattg aatatgaagt gatgagagat
    33301 tcggctgata atgcaatcgt tgtctgcaat atggaaaatt ttgacccggt gggtatccat
    33361 acaggtgatt ctatcgtatt tgcccctagt caaacattgt ctgaccatga atatcaaatg
    33421 cttcgtgatg cttctctttc gattattcga gcacttaaga tcgagggagg ctgtaatgtc
    33481 caactcgctc ttgatccaca tagttttaat tattatgtga ttgaagtcaa tccgcgtgtt
    33541 tctcgttcat ctgcgttagc aagtaaggcg actggctatc cgatcgctaa gctagctgct
    33601 aaaatagcgc ttggattaac attagacgaa atgaaaaatc ctgttacagg aacgactttt
    33661 gcagaatttg aacctgcatt agattatgtt gtagctaaaa ttcctcgctg gccgtttgat
    33721 aagtttgaaa acggagaacg gcttttaggc acgcagatga aagcaactgg tgaagtgatg
    33781 gctatcggtc ggaatatcga agagtcttta ttgaaagctg tgcgttcttt agaaatcggt
    33841 acacaccata tcgaacttcc tgagttggca gacatcacgg acgatgacct aatccaaaaa
    33901 gtggttcgag cacaagatga tcgcctgttt tatgtagctg aagctattag aagagggtac
    33961 accatagaag aattagcgga tttgacaaaa atcgatttat tctttttaga taaactgttg
    34021 catatcgtag aaatcgaaca gcagttgaaa cagcagccgt tggatgacaa gatattgaaa
    34081 acagcgaaac aaaacggttt tacagacaaa aaagtggcgg agctgtggga aataaacgaa
    34141 gaagccgttc gccagatgag aaaagaaaat gatttgattc ctgtatataa gatggttgac
    34201 acttgtgcag cggaattcga atcaaaaaca ccttattttt atagttccta tgaatatgaa
    34261 aatgaaagtt cacgatcaga aaagccctca attctagtac tgggatctgg tcctatccgt
    34321 atcggacaag gggttgaatt tgactatgcg actgtgcatt ctgtcaaagc aatccagcag
    34381 gcaggatacg aagcaatcat catgaacagc aatcctgaaa cagtttcgac tgacttttct
    34441 atttcggata aattatattt tgagccattg accttcgaag atgtgatgaa tgtgatcgag
    34501 ctagaacagc cgataggagt aatcgttcaa tttggtggtc agacagcaat caacttagcc
    34561 gaacctttga ctgctgctgg tgtaaaaatc ttagggacaa cgatcgaaga cttggatcgt
    34621 gcggaaaatc gtgatttatt cgagcaagcg ctgaaagagt tggatattcc tcaaccttta
    34681 ggggatactg ctacaagtaa agaagaagcc gtggttatcg cttcgagaat tggttatcca
    34741 gttcttgttc gtccaagcta tgtattaggc ggacgtgcga tggaaatcgt tgaaaaccag
    34801 cgagatttag aagattacat ggaacatgct gtgaaagcat ctccagaaca tccagtacta
    34861 gtggaccgtt acttgcttgg tggtgaatgc gaagtggatg cgatttgtga cggtgaaaca
    34921 gtactcatcc cagggatcat ggagcatatt gagcgtgctg gtgtccattc aggggattca
    34981 atggctgtgt atccacctca aacattatct gaatcgatca agaaaacgat cgaagattac
    35041 acgatccgct tagcattagg tttgaattgt gtcgggatga tgaatatcca gtttgtcatc
    35101 catgaaaatg aggtttatgt gatcgaagtc aatcctcgag caagtcggac tgttccattc
    35161 ttaagtaagg tgacaggaat tccaatggca caaattgcga cgaaagctat tttgggagag
    35221 aaactaaaga cacttggtta tgaaaacgga ctttatccag aaacaaaaga agttcatgtt
    35281 aaagcacctg tcttttcttt tacgaaattg cagaaagttg atacttactt agggcctgaa
    35341 atgaaatcga ctggagaagt aatgggatct gacagacaat tagacaaagc tttgtacaag
    35401 gcattcgaag cttctggatt acacttgcct gaatatggag ccgtattatt tacgattgcg
    35461 gacgaaacaa aagaagaagc actagaatta gctaaacgtt ttacagaaat tggctatagt
    35521 ctgataggaa cgaaacatac agctgcttat ttcgaaaaaa atggtctggt cgttgaaaca
    35581 gtagcgaaaa tttctgagga agcagcagaa aaaaatgtag tcgacttaat aagagatggt
    35641 aaaacacaag tggttgtcaa tacgattgat aaagatcggg gaaatgcttc taaagacggt
    35701 ttcatcatcc gaagagaagc agttgaacat ggtattccat tgtttacttc tttagacaca
    35761 gcagatgcga tcgttcgagt gatggaatcc cgtgcttttt caactaaccc tatttaacgg
    35821 cgagcacatg ttaagacaaa aagacgaaaa accgttgcag gaggggacag atgaaacaag
    35881 aaatgatgga aatcgttcag caaaaacagc tggcaccaaa gatttttgaa atgacattga
    35941 aaggtgatct agtaaaagaa atgcagcaac caggacagtt cttgcatatt ctcgtgccgc
    36001 gtgcagactt gctattacgc cgaccgatta gcttgaatca tatcgaccac gaaacaaata
    36061 cttgccgtat catttatcgg gtggaaggtg aggggactag agtcttctct actctttctg
    36121 ccggagacca tttagatgtc atgggtcctt taggcaatgg ttttgatttg tccaatcttc
    36181 atacaggaga cgaagtattt ataatcggtg gaggaatcgg tgtccctcct ctatatgagt
    36241 tatcaaaaca gttagtcgaa aaaggaatta agccaacgca ttttttaggt tttgccacac
    36301 atgaagtcgt ttattatgaa aaagaattta atcgtctagg agaaacaaga atcgctactg
    36361 acgatggaac atacggtatg cacggaaatg tcggtaattt attgttagga acgaagacgg
    36421 aacctacagc tgtttttgca tgtggttcaa acgggttatt gaagacagtg gaacagctgt
    36481 tttcttcgca tcaaaatgtc cagttatcat tagaatcccg catggcatgt gggatagggg
    36541 cttgctatgc ttgcgtttgt catacgccgg aagatgaaac aggaacaaaa agtgtgaaag
    36601 tttgtgacga aggaccgatt tttaaaatag gagaggtaat tatatgaatg agaatccttt
    36661 agcggtcaaa cttcctggcc tagaattaaa aaatcctatc atgccggcaa gcggctgttt
    36721 cggttttggc aaagaatacg gaaaatacta tgatttgaat cagcttggat cgatcatggt
    36781 caaggcaacg actccaaatg cccgctttgg caatcccacg ccaagagtgg cggaaacacc
    36841 atctggcatg ttgaatgcaa ttggtttgca aaatcctggt ctagacgttg ttatgcataa
    36901 acttcttcca gaattagagg agtacaatga gttacccatc atcgcaaatg ttgctggtgc
    36961 ttgtgaagaa gactatgtag aagtttgtag caaaattggc gaagcaccaa atgtgaaagc
    37021 aatcgagctg aacatttctt gcccaaatgt caaacatggc ggaattgctt ttggaacaga
    37081 ttctgatgtt gcttttcaat taacacaagc agtaaagaaa gtttcttctg ttcctgtcta
    37141 tgtcaaattg tccccaaatg taacagacat cgttccaatc gctcaggcaa tcgaagctgg
    37201 aggggcagat gggtttacga tgatcaatac tcttcttggt atgcggattg accttaagac
    37261 aagaaaaccg attttagcca atcaaacagg tggactctcc ggaccagcaa tcaagcctgt
    37321 cgctattcgt ttgattcacc aagtagcaag tatatccagc cttccaatca ttgggatggg
    37381 cggcgtgcag acagtggatg atgttcttga gatgtttatg gctggagcaa gtgctgtagc
    37441 tatagggacc gcgaatttca ccgatccgta tatctgtcct aaactaatta aagagctccc
    37501 tgtgcgaatg agcgaactag gcatcgaatc gctggaaaga ttaatcaaag aagtgagaga
    37561 ggaacgtgaa cgatgaatca actagaccaa cgaccaatta ttgcattaga tttttcaact
    37621 aggcaagagg tagaagactt tcttcgattt ttcccgaaag aagaaaaatt gtttgtgaaa
    37681 atcggaatgg aattgttcta ccaagaagga cctgaaatcg tacgttatct aaaagaggct
    37741 ggtcataatg tatttttaga tctgaaactt cacgatattc cgaatacggt cgaaaaagca
    37801 atgcggggat tggcaaagct tggtgttgat ctcacctgtg ttcatgccgc tggcggtatt
    37861 cgtatgatgg aggcggcaat gcgaggatta gaagagggga ctccagaagg tggaaaacgt
    37921 ccgctacttc tagctatcac tcagcttact tctacaagtg aagaagaaat gcatgccgat
    37981 caactaatcg aagtttcttt agaaaaaagt gtgatccatt atgctagctg tgccaaaaaa
    38041 gcaggattag atggtgtggt ttcttctgct tgggaagtag aagcgatcaa agaaatggca
    38101 ggagacgaat ttgtttgctt aactccgggt ataagaccag aaggaacagt cgcaggagat
    38161 cagacaagag tagtcacacc ttctcaggca aaaaagatcg gctcaacttt tatcgttata
    38221 ggtcgaccaa tcactcagtc aactaatcct tataaagcat atcaaacgat tcaaacagaa
    38281 tggagctaac ctaaaatgaa tgttgaacaa agtatcgcaa aagacttatt agaaattgaa
    38341 gcagtatttt taaatccaag taatcctttt acatgggcaa gtggaatcaa aagcccaatt
    38401 tactgtgata accgaatcac gatgagctat ccaaaagtcc gaaaagagat cgcaaaagga
    38461 ttagcaagca aaatcaaaga agcttaccct gaagtacaag tgattgctgg aactgcaaca
    38521 gcaggaatcc ctcatgccgc atgggtagcg gaaatactgg atttgccaat ggtctatatt
    38581 cgaagtaagg cgaaagatca tgggaaaggt aaccaaatcg aaggacgtat atttgaaggt
    38641 cagaagatgg ttgtgatcga ggatttgatt tctacaggcg gaagtgtctt ggaagcagct
    38701 gaagcagcaa aaagagaagg tgcggatatc cttggtgttg cggctatctt tacgtatgag
    38761 ttgccaaaag gaaaagcaaa tttcgaaaaa gccgaaatcc ctcttatgac gttgaccaat
    38821 tattctgtat tgattgaagc agctttagaa gatcgttata tcgatgagca agaattaact
    38881 ctgctaaaag aatggaaaaa agatcctgaa aattggcaga ttgaagcgcg cttgatgaag
    38941 atagagaaat ccgatttgtg tctccagaat catcaattgg aatagcaaaa atccaaaaac
    39001 tgattgcgag aagcatcttt tcatcaggta tgaaaagaaa cttctatcgt gatcagtttt
    39061 tgattttttt aaaattcatt tttctaagac tggttagcag aaagaagaga ataatctctt
    39121 ctcttttttc tattatatat agattttctc tattttttgg taagggagct gtttcaaaaa
    39181 tggtaaagag cggaaatgtt gcgaatatca gttatttaat gatatgatat tcggaaaaag
    39241 aaatctcaaa gggaggtaac ttttatgggg ttgaatgaat ttattttagg attgaacaat
    39301 ttagagacga tcacaagggc acctggattt tttaaatata cagagcatac agttgcagca
    39361 cattcgtatc gtgtagcatc tattgcgcaa gttctaggag acattgaaga agcgaatgga
    39421 caaccgatcg attggaaact gctctatgaa aaatcattga atcatgacta cacagagcgt
    39481 tttattggtg atatcaaaac gccggtgaaa tacgcaagca aagagttaag agggatgctg
    39541 caaacagttg aagaaaagat gacggacgaa ttcatcaacc aagagattcc agctgagttc
    39601 caagaagttt atcgccgtcg gttgttcgaa ggaaaagatg aaaccgtaga aggagaacta
    39661 ttagccattt cagataaggt tgaccttctt tatgaatctt ttgatgaaat catcaaaaat
    39721 aacccggaaa aagtttataa ggagatgttc attgaggcag tgactacgat taaaagcttt
    39781 gaacatcggc cttctgttca gtatttcttt aaagagatat ttccggaact attgaaccag
    39841 gaattttatg gaaaagaggc gtttttagaa accgtctcaa cgattgtaaa gaaagaagaa
    39901 aaataaaaat gatatgagat gcagggggaa gtatagttga aaaagaatat ggatgtcgaa
    39961 tggggctatt ctggagacaa aggtccggaa aattgggcga gtttatgtga ttggtttgcg
    40021 agaggtgcgg aatttccatt gcagtcgcct attcatttga ccaaggggga agaaaccaaa
    40081 atagaaggag acacgctttc ctttcattat caaaagcaac ggtttactga caaggaattc
    40141 aaaaacacga ttcatttagt accttttgac caactcagtt atgtagagtt caagaaagaa
    40201 cgctattatt tgacagatat tcatttccac ttgccttctg aacatatcat caacggaaaa
    40261 caggaagata ttgaatttca ttttgttcat atgaacagca aaaaagagaa tctagttgtg
    40321 ggtgtcatgt accagctaat ggtacaggaa ggttggctgt ataatcaaga aaatggggaa
    40381 agttggaact tagaaaaaca tgagcattgg gtaaatccgg atgtgttctt tccggagcaa
    40441 aaaagccatt tccattatat tggctcattg acaactccgc ctaccagcgg gccaatccaa
    40501 tggtttgtaa tggatcatgt aggaaaactg aaccaagagt ttcttctccc cttcgatggt
    40561 cagtatgctc gaccgaacaa ccgaccaatc cagcccttga atggaagaga aatttattat
    40621 ttcgaggagt ttgagcaatg aatattcagc agatgaaata cgtggtcgct gtagcaaaca
    40681 atggtagttt cagagaagcg gcaaagaaaa tgtttgtctc ccagccaagt ctttcagctg
    40741 gtatcaaaga attagaaacc gagttaggga tcacgctgtt tactcgaacg aatcgagggg
    40801 catatttaac agaagaaggg caagacttct taaatcgtgc cgagaaaata ttggtccagc
    40861 ttgatacatt agaaactcac tatctaacga gtgaaaagag agacaatttt tcgatcgctg
    40921 cccagcatta tgatttctta ggtcctttga ccgcacagct actcgaggaa ttcaaagacg
    40981 agatcaaaca atttcggatc atggagacga caacagcgag agtgatcgaa gaagtcaaag
    41041 agcagcacag tgagatcggc attatttata tgaatgagca taatcaagct ggtatcagaa
    41101 gatatttaca acaaggagag ctaagtggcc gcagcattgg aaaattcaaa acgcatattt
    41161 ttttaaggca aggacatcca ttagctgaac acgcagagat caccaaagaa gaattgttat
    41221 cttatcctca agttcgtttt acacaggatg gaaataattt cccgtatttt tatgaagacc
    41281 ttatcgagat ccctgatcaa gaaacagtga tttatacgag tgatcgaggt actttgatga
    41341 atatcgttct tgaaactgat gcttacgctt caggatctgg tattgtgatt ggagggattc
    41401 gcgagcactt gcggttaatc cctttggcag aaggcgaact aaatgaattt tatatcattt
    41461 attcggcgaa acgtccgctc agtgaaatcg ctcaaagatt tatcaaagag cttactcggt
    41521 tactggacca gcagagctaa gaaaagcaga tgagaacgct gtttattgaa caccgttgat
    41581 caaaaaagag gggttgtgac agaattttgt cacaactcct ctttttgtag caaacaattt
    41641 ataactaatt gctgttgtgg gaaagtacgc aaatatgatt ctagaaacac tcattgatta
    41701 cctgttttgt ttcatagctg taattttttc ttcttctggt gtcacgataa tcgtcttttg
    41761 gttttcataa ataacataac caggcttggc gccatttggt tttcggatat gctttacctg
    41821 tacaagatca accggcactt gggcagagta tcggtatttt gaaaaatagg cagctaactc
    41881 ggcagcttct gtaattgttt catctgaggg cttgtcactt ttaatgatga cgtgcgaacc
    41941 gggtatattt tttgcatgaa gccaatagtc ggttttttta gctgtcttca ttgtcagctg
    42001 gtcattttga agattgtttt taccaacaag aatgagcgtt ccgtcagaag aaaagtattg
    42061 atcaggctga gatggtttct tacgtttttg tttttttaag ttcttctttt tcaggtatcc
    42121 ttctgaagtc aattcttctt taattgcttc gatatccatt gggccggcaa tttccagttg
    42181 agataagaca gattcaagat attggatttc atctttggct tcttggattt gttcgccaat
    42241 cagcttaaca gcgttcttca gcttttggta tcgatggaaa tatttctgtg cattttgatt
    42301 tggtgtcaat gccggatcta gtgctatttt gattggccga tcttcttcat aataattagg
    42361 taaaacaact tcttttgcgc cgcgtggtac ttgtgtcaga aaagtagtaa gtagttcgcc
    42421 atttctacgg taattctcag cgttttcgga ttctttgagt gtttgttcac gttttttcaa
    42481 tttattttta tttcgtttta gttcattttc gatttttcga atcagttctc cgccttgttg
    42541 ttttgcccga tctttctctg ctttttcgtg ataatagcta tctaataatt gactgagtgt
    42601 tggataagct gttacagcag atgcctgttc ggacaatacc tgataagcaa tcggtgtgaa
    42661 aaaatcttta ttttccgttt cgtaaaaagt aggaataggc tgatggctga tggctgagaa
    42721 aaattcgtcc catacaacca tcttttcatt aggacgttcc agtaatcttt tggtcagctc
    42781 ttgagcgata tcgaaaccga ttccctgaaa ctgacgttgg attgcttttg gatctagttc
    42841 agtttgggaa agccgctgaa agattttttc tttctcggag ctaaatgggt tcaagacctc
    42901 ttgttttggc ggagcaacat attctacacc aggaagcaaa gaacgataag tgttttgaga
    42961 gcttccaatg tgtttgatcg catccagtat ttttccagtt tctcgattaa ctaagacgat
    43021 cgtactatga cgtcccatta attcaacgat caatatgata ttttgtagat cacccaactc
    43081 gtttcgttta gcaaagtgga aatggatcac tcgatcattt tcgatttgtt cgatgtcttc
    43141 taaaatagcg ccatccaaat attttcgaag catcatcacg aagttaggcg gattatcagg
    43201 attctgataa tcgatttggg taatctgtac acgagcataa cttgggtgcg cagataataa
    43261 taatcgatgg tttttgccgc gtgaacgaat caccagcacg atttcatttt cataaggctg
    43321 atggattttc gagatccggc cagatagtaa cgtttcacgc aattcgttga ccatcgcgtg
    43381 ggtaaataca ccatcaaagg acatggttca ttcctcattt cttttcataa gtatattcat
    43441 tataacgaaa aaaagaagaa aaaccaattt ggccgaatag taaagcaaaa gtctagaaaa
    43501 atcttggact aattacataa aaaacagtgt ttctatcgtt gaaatccaaa tttttaggta
//
LOCUS       DAJSBN010000021        42721 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJSBN010000021 DAJSBN010000000
VERSION     DAJSBN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.021"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            215..1243
                     /locus_tag="ONT86_001246"
     CDS             215..1243
                     /locus_tag="ONT86_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR2352054.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1299..1517
                     /locus_tag="ONT86_001247"
     CDS             1299..1517
                     /locus_tag="ONT86_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352055.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1588..2403
                     /locus_tag="ONT86_001248"
     CDS             1588..2403
                     /locus_tag="ONT86_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352056.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            2467..2652
                     /locus_tag="ONT86_001249"
                     /pseudo
     CDS             2467..2652
                     /locus_tag="ONT86_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
     gene            3226..3489
                     /locus_tag="ONT86_001250"
     CDS             3226..3489
                     /locus_tag="ONT86_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352057.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
     gene            3823..4194
                     /locus_tag="ONT86_001251"
     CDS             3823..4194
                     /locus_tag="ONT86_001251"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR2352058.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            4374..4796
                     /locus_tag="ONT86_001252"
     CDS             4374..4796
                     /locus_tag="ONT86_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352059.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            4941..5186
                     /locus_tag="ONT86_001253"
                     /pseudo
     CDS             4941..5186
                     /locus_tag="ONT86_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5295..6251
                     /locus_tag="ONT86_001254"
     CDS             5295..6251
                     /locus_tag="ONT86_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352060.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(7087..7758)
                     /locus_tag="ONT86_001255"
     CDS             complement(7087..7758)
                     /locus_tag="ONT86_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2352061.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            8075..9706
                     /locus_tag="ONT86_001256"
     CDS             8075..9706
                     /locus_tag="ONT86_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR2352062.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            9709..10788
                     /gene="uxuA"
                     /locus_tag="ONT86_001257"
     CDS             9709..10788
                     /gene="uxuA"
                     /locus_tag="ONT86_001257"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR2352063.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            10862..12289
                     /gene="uxaC"
                     /locus_tag="ONT86_001258"
     CDS             10862..12289
                     /gene="uxaC"
                     /locus_tag="ONT86_001258"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR2352064.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            12373..13020
                     /locus_tag="ONT86_001259"
     CDS             12373..13020
                     /locus_tag="ONT86_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR2352065.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            13149..14525
                     /locus_tag="ONT86_001260"
     CDS             13149..14525
                     /locus_tag="ONT86_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2352066.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            14536..15330
                     /locus_tag="ONT86_001261"
     CDS             14536..15330
                     /locus_tag="ONT86_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2352067.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            15343..17109
                     /locus_tag="ONT86_001262"
     CDS             15343..17109
                     /locus_tag="ONT86_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HCR2352068.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            17137..18945
                     /gene="uidA"
                     /locus_tag="ONT86_001263"
     CDS             17137..18945
                     /gene="uidA"
                     /locus_tag="ONT86_001263"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR2352069.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            19117..19518
                     /locus_tag="ONT86_001264"
     CDS             19117..19518
                     /locus_tag="ONT86_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2352070.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            19512..19757
                     /locus_tag="ONT86_001265"
     CDS             19512..19757
                     /locus_tag="ONT86_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2352071.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(20081..20302)
                     /locus_tag="ONT86_001266"
     CDS             complement(20081..20302)
                     /locus_tag="ONT86_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352072.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(20464..20536)
                     /locus_tag="ONT86_001267"
     tRNA            complement(20464..20536)
                     /locus_tag="ONT86_001267"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20501..20503),aa:Lys,seq:ctt)
     gene            20612..20842
                     /locus_tag="ONT86_001268"
     CDS             20612..20842
                     /locus_tag="ONT86_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2352073.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            20886..22055
                     /locus_tag="ONT86_001269"
     CDS             20886..22055
                     /locus_tag="ONT86_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2352074.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            22629..23198
                     /locus_tag="ONT86_001270"
     CDS             22629..23198
                     /locus_tag="ONT86_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCR2352075.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            23185..24045
                     /locus_tag="ONT86_001271"
     CDS             23185..24045
                     /locus_tag="ONT86_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2352076.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            24235..24939
                     /locus_tag="ONT86_001272"
     CDS             24235..24939
                     /locus_tag="ONT86_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2352077.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            24944..26779
                     /gene="walK"
                     /locus_tag="ONT86_001273"
     CDS             24944..26779
                     /gene="walK"
                     /locus_tag="ONT86_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCR2352078.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            26776..28092
                     /locus_tag="ONT86_001274"
     CDS             26776..28092
                     /locus_tag="ONT86_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352079.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            28093..28962
                     /locus_tag="ONT86_001275"
     CDS             28093..28962
                     /locus_tag="ONT86_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCR2352080.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            29017..29826
                     /locus_tag="ONT86_001276"
     CDS             29017..29826
                     /locus_tag="ONT86_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR2352081.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(29929..30558)
                     /locus_tag="ONT86_001277"
     CDS             complement(29929..30558)
                     /locus_tag="ONT86_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR2352082.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            30728..32020
                     /locus_tag="ONT86_001278"
     CDS             30728..32020
                     /locus_tag="ONT86_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2352083.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(32096..32758)
                     /locus_tag="ONT86_001279"
     CDS             complement(32096..32758)
                     /locus_tag="ONT86_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR2352084.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            32983..33267
                     /gene="groES"
                     /locus_tag="ONT86_001280"
     CDS             32983..33267
                     /gene="groES"
                     /locus_tag="ONT86_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR2352085.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            33318..34943
                     /gene="groL"
                     /locus_tag="ONT86_001281"
     CDS             33318..34943
                     /gene="groL"
                     /locus_tag="ONT86_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR2352086.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(35032..36180)
                     /locus_tag="ONT86_001282"
     CDS             complement(35032..36180)
                     /locus_tag="ONT86_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2352087.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(36282..36866)
                     /locus_tag="ONT86_001283"
     CDS             complement(36282..36866)
                     /locus_tag="ONT86_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCR2352088.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(36993..37424)
                     /locus_tag="ONT86_001284"
     CDS             complement(36993..37424)
                     /locus_tag="ONT86_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR2352089.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(37442..37762)
                     /locus_tag="ONT86_001285"
     CDS             complement(37442..37762)
                     /locus_tag="ONT86_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2352090.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            38062..38202
                     /locus_tag="ONT86_001286"
     CDS             38062..38202
                     /locus_tag="ONT86_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352091.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            38500..38757
                     /locus_tag="ONT86_001287"
     CDS             38500..38757
                     /locus_tag="ONT86_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HCR2352092.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(38728..39036)
                     /locus_tag="ONT86_001288"
     CDS             complement(38728..39036)
                     /locus_tag="ONT86_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352093.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            39114..39860
                     /locus_tag="ONT86_001289"
     CDS             39114..39860
                     /locus_tag="ONT86_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCR2352094.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            39875..40078
                     /locus_tag="ONT86_001290"
     CDS             39875..40078
                     /locus_tag="ONT86_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2352095.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            40183..40482
                     /locus_tag="ONT86_001291"
     CDS             40183..40482
                     /locus_tag="ONT86_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352096.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            40482..40664
                     /locus_tag="ONT86_001292"
     CDS             40482..40664
                     /locus_tag="ONT86_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352097.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(40670..41146)
                     /locus_tag="ONT86_001293"
     CDS             complement(40670..41146)
                     /locus_tag="ONT86_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352098.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            41199..41924
                     /locus_tag="ONT86_001294"
     CDS             41199..41924
                     /locus_tag="ONT86_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCR2352099.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            42098..42439
                     /locus_tag="ONT86_001295"
     CDS             42098..42439
                     /locus_tag="ONT86_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352100.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            42432..>42721
                     /locus_tag="ONT86_001296"
     CDS             42432..>42721
                     /locus_tag="ONT86_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR2352101.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT        14832 a         6415 c         8419 g        13055 t
ORIGIN      
        1 agtttagcta gggcgcttat taaatcagct tcagttatta tattagatga accatttaga
       61 aatttagata atgaaactcg cgataatttg agggattata tactaaatac aaaaaaagaa
      121 gtttgttata taattataac tcatgatcac attctagata attttttgga ttcaaatgat
      181 gaaatattat atttaaataa ggagtatgat aataatgaat agcaaaaaac ttcagatggg
      241 tgaaattgtt agaggtccta gtcaaatatc tattgatgtt acatataaat gtaattttag
      301 atgtaaacat tgttacaatg cgagtggtgg agacaattgt tttatggata atgagttgac
      361 tgatgatgaa ctgttagaac ttacaaaaga tatatgtaat ttaaagccat ataatatttg
      421 tttctgtgga ggagaaactt tactgagaaa aaaaatagtt ttatcttgtg ccactttatt
      481 aaaagaggca ggtattccta atattgctat tgttactaat ggatcattag taactcagga
      541 tgttgcaaaa gaattattca aagcaggtat taatcgattt caagtaagtt tagatggaaa
      601 aaatgctagt agccatgaag cacttagatt atatccaaac agttttgaaa aggcaattaa
      661 tgctatcaaa ttatttagag aattgtccga agaagtggat gttgcttttt gtcctactag
      721 ttttaattac aatcaattac gagaagtata tcaactagta aatactctta aagtgaagtc
      781 tttaagaatt caaccattta tgcttagtgg taggggaatg caaaataggg aaatagtgcc
      841 aactgtttct caatatagaa aactagtttc tgatattaac tatattaaaa gaaatgaaga
      901 ttataccaag atagattggg gagatccaat tgatcatatc atccgacaca gaactttatt
      961 gaaagatttt tcgaattttg tttctattag gtcagacgga ggattaatgg catcacctta
     1021 cttgccaatt caagttggaa atattaaatg tcacagtctt tctaaatatt ggcgagcagg
     1081 actttataaa gttttttctt tagatttagt tcaagatata gctactcaag tttcttcaat
     1141 acaagatatg agtaaagaat taaaaaaagg acatattaaa tggcttaatg aagatataga
     1201 aattgatatt ctcgatgaat acaaacatga tattttaatt taggtttatc attggaaagt
     1261 ttagataaac aaagtacttt tagaaaggag ggaaaaaaat gatttctgaa cctcaaatga
     1321 ataatatgta tgaaagccca actataatgg gagctggtga agctgcgcca agagcttgtt
     1381 cagcaattat ggtgtgtgtg ttattagcat ctgcaggagt aggtatccaa gcaggagcgg
     1441 tgtatactta tgctgtagtt gctacagtag ctgtagctgc agccgcagtt tggaaaacag
     1501 taggattagt tggttaggaa ttaataggag actaactgtc tcctattgat tccaatgatt
     1561 aagctaagga ggaattttaa gaaaataatg aaatacaata atttaaaacc gaaattactt
     1621 aatgagaaca tagaatatat aagagaagaa aattatggat atttaaccat agttcccaaa
     1681 aatggaactg aattgaaaga taacgttttc aatccaacag taagatatat attggctaac
     1741 tgtactggaa aaaacagtgt aaaaaaaata gcgaatggtt taaaaaagaa gtttcctagt
     1801 ttagaatatg aaaaattcta tcaagatgtt gtaggaacta ttaaattttt caaacactat
     1861 aatattattg atataggaga ttatagtatg gaagaaaaga aaatatataa aattgaacaa
     1921 aatttagaat tagaattact aaatgaatca cattatgaag aaatttataa ttttatcaca
     1981 gatcatcctg aaagtatgta ttttcatgaa gatatttatc atcaaggaaa tagtaaagaa
     2041 ttagaagttg tccttagata taatttattt tcgtttacga gtgaatttta ctttttaaga
     2101 cataaagaga gaattgtagg aatggtttct ataattgtta atccatttca aaacttatcc
     2161 tacagtatag taaattacct catatgtgag cctgcatata ctacgactat tttgacaaca
     2221 gtattagaaa atattaacga agtttcaatt ttagaagtaa agaagctagt gattcatttg
     2281 ctaaattcaa cagaagatga atcactaatc ccctatttaa aagattcaca tttttttaaa
     2341 gaagcggtac tatctaaaga atatagagag catgatgttt tgagatacgc ttacatttat
     2401 taaataaaat ttctgatagt attttaataa ttcggatcat atcataagat ataggaggga
     2461 aaagaagtga ttttattgat tttgtctctc gtattactag ttgtatctaa tctaatgaaa
     2521 aataatcctc ctacaaaaat taattctttt tatggcttta gaactaaaaa gtcaatgaaa
     2581 aatgaaaaaa gttggcattt agcacaaaaa ttgttggggg atttttcaaa aaaaaaagtt
     2641 atcccagtat ttttgatggg tattgttttt ttcagtatag agatttatag tttagctatt
     2701 ttaaaaaatg aaaatttttt cgttagtgta ttaatgggtg aaagtttaat cctaatactg
     2761 attttgtgtt taattgttca tgaagtgaat aaacaattgt gaattaaaaa aataaaaacc
     2821 tagtataatc actttaataa attgttggta gaaggtgatc aaaatgggag taaacgaaat
     2881 gatgaaagtc agattgagtt tttaaatgat accttcctca gaacttcaaa acgaattcaa
     2941 agattcgttg acaaatcttg agcaagatca tttaaaaaaa tattttctct tcatttgacg
     3001 aaaaatgttt agaaatctta tataagtgaa gataaagtaa ttggtgtaat taattgtaga
     3061 gaatagcctt tgagcaattg aagcgataca attatttaga aaatgaactt cctccagcat
     3121 actacatgat tccttacggg acgatgttaa agaagaaagc aagaaatttt agaagtatta
     3181 gtagataaac taatgggctt ttctcaattt aagggagtag cccaaatgtt tatcaaatat
     3241 ggtgcagata cagtagcaac catgtttaca gaagaatatc aagtagacta ttatgaagta
     3301 atagataaat gtgtacattt tgtttatgaa aaagtgtgga attttatgaa ttttgcgata
     3361 agtgaagaaa tcttacgagt acaagaaaac agaacaggaa tatttaccag agacagaaag
     3421 cttaatttga ttgcgcatta tatacggaat cgctggtcat caataccaaa ttttaattat
     3481 aaaaaataat tatgaacaat cttttataca atatacacaa taaatcaagt agtatttatg
     3541 aaaaaagact tcaaaaacag aatgattttc tatcagtctt agaaattata gagctaaaaa
     3601 ttttggtcac gcgtagaaac gcgttttttt tgtttttttg atagtaaaga acgtacaaaa
     3661 caatgagaat ttaaaagatt gtactaccag tttttttacc ttttcatttg agtttagagt
     3721 aagtaaaata ttactataaa aacacatatt cttgaagaaa tctttttttt gctgtattca
     3781 tttagattat gatagagaaa atacgtgaga gggggacctg ggatgtggct atctgatgaa
     3841 aaaattcagt ggcgtagcta tcagaagcgt atattgacta actttgctac ttatttaaat
     3901 aacaattcag tccatctagt ggctccacca ggggctggca agaccttttt aggaattgag
     3961 atgatccgac gtttggattg taaaacctta attttggttc cttccctaat attgaagaaa
     4021 cagtggttaa caacaatcga ggaatatttc ttagtaaatc cagcagatag ttgctatctt
     4081 tctggggcga ttgaacatcc tagtcatatt acagtggaaa cgtatcagac catttacaac
     4141 cgactgaagg aggagccaac ttatggtgat cagctggagc tgttagtttt ttgatgaagc
     4201 tcatcattta aaaaaaagct ggggtgaatt gttgattgac ttgaagaaaa aacaaccgaa
     4261 tctgttgacg atttccttga cagccacgcc tccgattgat agctctcaga cggagtggaa
     4321 gcaatatatt gagttaaaat ggcctgattg acgaagaaat tagcattccc gagttggtaa
     4381 aggaggaggt tctatgtcct catcaggatt ttctatattt gattccggct aatcaggaaa
     4441 ctactcagca ttattttacc tttttagaga aacaaaatga gctggtggaa gcgttaatgg
     4501 aaagccaaga gacggtggat tatttgcttt ctttagaatt tgttcagcat ccgcttgagc
     4561 attcgaagga aatttatgaa aattttgagc tgttcactag tgcattaatt tacttgaatc
     4621 atcataacta tgaattgata cccgaacatt ggcagctatt aggcagttct aagcagcaag
     4681 gtaaaaaagc ttcagttcca tctttaggta aaaaagagct gatcactatt tatcagcaga
     4741 tgtttgaaga tcaaccggaa ttgcctattt tttatcaatt gaaaaaagct ggatgattgt
     4801 ttgatgagtc gctgcatttg tttccagagt atccagacac tgttggtagt caggcgattc
     4861 ctgagaaaaa gcaagcagtt tgtcaaattt tgttaaaaga agaagttcag ctgaagcaag
     4921 aactgacagc ggtcattttg atggatcgta tttgcaagga ggtattagca ggcagtgatg
     4981 gtaccttagc ttatggcgtg gttcctttgt ttgatgaagt tgttcgtcta ttaggtaaca
     5041 ctcagcaaat cggtgccatc tgtggagagt tcttactgtt accgaaaatg ctagtaactg
     5101 ttgagctggc agaactagcg acgggtatga atgaacatcc ttatatgtcg ggttattttt
     5161 atttgacggt ggatgacaaa aaccgccaag cattgatgca agcagttact cgcctattaa
     5221 ccgaaaagaa gttgaatatt ttggtaggaa ccgttagttt gctaggagaa ggctgggatt
     5281 gtcctgcaat taatctgttg atcttggcta atcaagcttc cacttttgtg caaacacaac
     5341 aactacgagg gcggggctta cgcctaggtg cacaaaataa agtaacgaac atttggcatt
     5401 tagcgattgg attaccggga gttcctctgt ctgaacagcc tgatttgaaa agaatgctac
     5461 ggcgtttggg caatatttca ggtctgacct ttgaatcttc accaaaaatt gagagtggtg
     5521 gcgatcgctt tgctttacca ctggaatata cgaccaccac gatggaaact tatactcagt
     5581 cgatgttcta tttttcttgt gaacgaagtc ggttgaaaga acaatggagt actgctttag
     5641 ctgatggctg gcacctatct atgccaatcg tcgttaggaa agtcgatcag gtaaagcaaa
     5701 aacaagaaac ttcattgccg atagtagtaa aaaatataga gcctctaagc ttctggtcag
     5761 ctatatttaa aggtaacttg ctggaatatt tccaaattcg taggcgacaa aagctatggg
     5821 aaaaggaatg tgcccgcaaa aaaagcttgg ccatatgcgt tcaaacgacg ttgcagcgcc
     5881 ttggtgaagt tagtgataga aaagtaatcg tagcggctga tcagcaggaa tttagatgct
     5941 atcttgagaa tgctggctat cgagaaggac agctttttac tgaagctatg agggagcttt
     6001 tagcaccgct ggagaatccg cggtatttaa tcgaagggaa aaagatagtt ctgacggtac
     6061 cagaacgctt tggccgaaat aagcaaatcg ctaacctttt tctaaacgaa ttaaatcaat
     6121 cgtcgctgcc caaatgtcag ttggtgtata ctagaaatcc tgaaggacgc caaaagctag
     6181 tgacttatcg aatgaggcaa gtcttccaga cccgcgatga agtaattatt caaaaaaaca
     6241 tttggacgta agttgatgaa aataaatttt gctagagtag aacaagtccg agaattaagt
     6301 tgctacttaa ctgataatgc caaggacagg agaatctttt cttgacgact gaaagaaact
     6361 atttgactta tataacggtg tgtgatgaat atttgcaagg cattctctta aaaagtactc
     6421 gtaatagttt ttttgaaaac aaaacagtac tttattaata ggactgacag agcgatataa
     6481 gaacatctgc agacagaggt gaatgctttt ctcatgatcg atgaaacaat ggtacttcgg
     6541 atattcttgc ttttagtttg aaccgttgtg attttctttg attcctaaaa gtagttttat
     6601 agaatgaaaa tcaagcatac ggaatgctcc tttgattgtg ttgtggtgat tcaatctaat
     6661 ggagcttcta tatgtttttt tatttgcaaa aagaaccgat agaatatttt tatcagtctt
     6721 agaaattata gagccaagaa atttcaagca cacataaaaa cgcgtgtttt atgcatttta
     6781 tgagcaaaaa taactaaaga ccattacgta aaacataggt aaatcagcat tcaatctcaa
     6841 attatgtgat caaatgttga tattatttga ttataagttt tcttttgcta ttatcagtct
     6901 gcaaataaac agattataga gtatgaatgt agatagttca atctttgttt acgacctcac
     6961 atgttttttt actcaagaga aagtaatata aagtgactat atttgacaga actttacata
     7021 aaaaaagtag ttagttttct gtagtaatca gaaatgtaaa taaattaaaa aaaagaaatt
     7081 tagcagttat atatttttaa aatattttgg gtgagtagtc aatacaacat tttgttcatc
     7141 aagcattaaa tgaagatgca aggatgttaa gaagttagct tcttcagggt cttgcatggt
     7201 caaggcttta aatagttgat gatgttgttc cataattttt tcccatttta atccttccgt
     7261 ttttactcgt aaccaacgaa atcgttccaa gtgcgtatta ttatcttcca accattgcca
     7321 tacttcttcc cgttctgcaa tttcaaaaca agctttatga aataaattat cattatgaaa
     7381 aaaactacga gcattctttt gactaacagc tttttcttgt tctgcaatta tagtttctaa
     7441 aacttgtttc ttttgattat tcatcttggc acaacactct agcataattt gtcgttctaa
     7501 atgttctctg acaaaacgag cattttttgc taaatctaag tcgattaatg agatataatt
     7561 tccactttga ggaattgtat caacaagtcc ttgatgacgg agttgaatca atgcttcacg
     7621 tattggagta cgaccgatag aaagcgtttc ttccaatttt ttttctgata ctttttttcc
     7681 aggttctaag tcagcgtaga taatttgctg acgaatagat tgataagctt gattttggag
     7741 actagtttta attgtcatat ttaatccctc tcaaaatttc atttttgaat ttgatatttt
     7801 agttctatgc tcgttatata atatcaaatt tcaaagtaac atactagcta ttaaaaaaac
     7861 aaaaaatact caaataagat caaagagaaa ttaagcttta atttaagtta gttctaaaaa
     7921 atatatattg gtgtgctttt actaaaaaaa gacacaataa ttctgacatg gtagaaaaaa
     7981 gaatattttt agttttttct tattgacagc gattacattc gattttatac tacaattgta
     8041 acggaaacta acatatcagt atggaggggt aaatatggtt ttgattaaag actggcaaga
     8101 ttctatcgaa ggattgaaca aaatgcaagt tcaactaccg tattttaatg tcgaagcggt
     8161 caaacaagaa ggtcagaaac atccagtgtg gcttcatttt ggtggaggaa atctttatcg
     8221 aggatttcat gcagaaattg ctcaatgctt agcagatcaa aatgaattag aatccggagt
     8281 agttgtgtgt gagacatttg atgaagaagt agtagaaaaa gcttatcatg actacaacaa
     8341 tgatattttg gagattgtta tgggtgaaga tggtgaactc aaaaaaagac ttttagcagt
     8401 aacagctgat agtttatatt gcaatccaaa gaatataacg gactatcaaa gagtggttaa
     8461 atattttgaa gatcctacat tacagtttac aacgtttact attactgaaa aaggatacag
     8521 tttaaaagat tctaaaggac aatttttttc ggtagtggaa gcagatataa aatctggacc
     8581 taccaatcct caacatacga tgagtattgt gacatccttg ttgtatagca ggtttaatgc
     8641 tggagagtta ccgattgcga tggtttctac tgataatttc tcacagaatg gtcaacgttt
     8701 tcaagatagc atttttatga ttgcagaagc ttggcaaaaa aaaggatacg tttctgaaaa
     8761 atttattgct tatttaaagg ataaagaaaa agtgactttt ccttggagta tgattgatag
     8821 aattacgcca aatccttcgg aaagtgtcaa aatggattta ttgaaggcgg gtttcgaaga
     8881 cttagatata attcatactg cgaaaaatac caatatcgct gcttttacca atactgaaaa
     8941 agtccattat ctagtcattg aagatagttt tccaaatggt cgacctaaac tggaagcggc
     9001 tggtgtgatt atgacagata gagaaacagt tgataaagca gatatcatga aggttacgac
     9061 atgtttaaat ccgctccata cggcattagc aataacaggt tgtttgttgg ggtataaatc
     9121 aattcaggca gaaatgcaag atgatgattt ggttggctta attaaaggga taggttatgt
     9181 agaaggctta ccagtagttg aaagtccagg cgttattgat ccaaaacaat ttattgatga
     9241 agtaatcaat aaacgtttgc ctaatcccta tattccagat acaccgcaac gaattgcaac
     9301 agatacttct caaaaagtag cgattcgcta tggtgaaaca attaaaaagt atgtcgaaaa
     9361 agacggaaat gcagaaaatc taacatttat tcctatcgct attgctgctt ggttacgtta
     9421 tttattggca gtcgatgatc aaggaaaaaa ttttgtgcca agtcctgatc ctttattggt
     9481 agagttgcag gaaaaattct cagtattgaa attaggtgaa caagatcctg caaagattca
     9541 ttgtgcggta caggaaatct tagaaaataa ggaaatattt ggtttagatt tatatcaagt
     9601 tgagatagga aagaaaattg aaaaaatctt ttgcgaaatg ctagtaggtc ctcaagcagt
     9661 tcgtaaaaca atcaataaat atataacaga agttggagga aagtaactat ggaaatgact
     9721 tttagatggt atggggcaaa tgaagaaaaa atctcactac aagaaattcg tcagatccca
     9781 gcaatgaaag gaattgttgg aacattaatg gatataccag ccggagaggt ctggccgaga
     9841 gaacgaatta aagctttaaa agatgaagtc gaagccaatg gccttacctt gaaagttatt
     9901 gagagtgtta atgttcatga tgatatcaaa attggtttgc caacacgaga tcaatacatc
     9961 gagaattata aacagacgat tcgcaatttg gcagaatttg ggattgaggt tatctgttat
    10021 aattttatgc cagtctttga ttgggtaaaa tcagatttgg attatcattt agatgatggg
    10081 tcttctacgt tagcctatat ttcaaaaaat attcctactg atccagagga aattattaga
    10141 aaagtagaaa attctactaa taattttgaa ttgccgggtt gggaacctca tcggttatca
    10201 caaatcaagc aattattaga agcctataaa gaagttgatg aagcgaaatt gcgagagaac
    10261 tttgcatatt ttctcaaagc aattatcccg acatgtgaag aagttgggat taaaatggca
    10321 gttcatccag atgatccacc atattctatt ttcggattac ctcgtattgt taaaaatcgt
    10381 gacgatttag aatggttgtg taatgtagtg gattctcctt ctaatggtat tacgttatgt
    10441 actggaagta ttgcagaacg accagataat gatgtctatg aaattctatc tgaattttgt
    10501 caacgggatc ggattccttt tgcacatgtt cgtaatatca aatatttaag tgataatgga
    10561 ctcgattttt atgaagcgcc gcataaatca gaatatggtt ctctagatat gtataagata
    10621 ttaaaaacaa tgtacgataa tggatttaat ggctatattc gtccagatca tggtcggatg
    10681 atttggggag aaaccggtag acctggttat gggttatatg accgagctct aggtgcagtt
    10741 tatttaaatg gtatttggga agcgcttgaa aagggggcga gtaaataatt atataatgga
    10801 gtctatttag aagaaaaatt ttctcttcta aaaactaagg gaaggaaagg agtgcttaaa
    10861 tatgttttta gataagaact ttttattaga gaatgaaata gctaaaagac tttttcataa
    10921 atttgcagaa caccaaccaa tcatcgatta tcattgtcat ttagacccag catgtttgct
    10981 agaaaataag caatacaaga atttgactca ggtatggtta aatgataaag gagctggaga
    11041 tcattataaa tggcgattga tgcgagctaa tgggatagaa gaagagttga tcagtggtgc
    11101 aggtaatgat tatgaaaaat ttttagcttt tgtaaaaaca gtggagaagg cgccaggtaa
    11161 tcctattttt gaatggtctc atttggaatt acaacgttac tttgatattg atttgacaat
    11221 ttctgagaag aatgcaccag aaatttggcg acgtgccaac gaaaaaatcc aaacggattc
    11281 ctatcgccca aaagaattgc tgaagatgat ggatgtgaaa gccatttgta caacggatga
    11341 tcctacagat gacttgcgat atcatactga gctttctaaa caggataatt ctttcaaggt
    11401 attacctact ttccgtccag ataaggtctg gagtatcaaa tcatcaggat tttcgaacta
    11461 tattttaaaa cttagtcaag cagcgggtat accaatcaaa aactttaatg ggttgaagca
    11521 ggcattgagc caacgagtgg attattttca tcaaattggc tgtcgattag cagatcacgg
    11581 tttgaatact tatttttatg aaatagcaga tgaaaaaata ctcgacaatc tgttaataaa
    11641 agctttagca ggaaaaacgg atttccattt atcagaaatt gcacaatttg ttacaggtat
    11701 tcaattacat ttgatgtctg agtatacaag atatggttgg acgatgcaaa tgcatttaaa
    11761 tgctttaaga aataattcaa gtatattaaa ggaacaaatt ggagtagatg tcggtggtga
    11821 ttcgatgggg gatcaagcta gtttgacaca aaatgttgta gcattgttcg acactgctga
    11881 aaagagtggg tgcttaccaa aaacaatttt atattcatta aatccaaccg attggcttcc
    11941 actagcaaca gcaatgcaaa gcttccaagg gggtatgatt caaaaaatcc aattgggatg
    12001 tgcttggtgg ttcaatgata cagctgaagg aatgcgtcaa cagttgacta cgatggcgca
    12061 acaaagtttg ttgtcgaatt ttacaggtat gttgactgat tctcgaagtt ttctaagcta
    12121 tcctcgccat gagtatttcc gtcgagtatt atgtcaatta ctaagcgagt gggttaaagc
    12181 aggtcgaatt ccagatgatg aaatgtatct tagtcagata gtggagaata tttgttatcg
    12241 taatgcgtat aattatttta atttttttga aatcgaaagt agaaaataat acaaaaaata
    12301 aaagcttaaa tttatttatt gtaagttagt acaactagct agagtatgta tccataaaag
    12361 aaggagatag atatgaaaaa agtagaaatt ttaagtaagt tagaagcggc tggagtcatt
    12421 gcggttgtac gtggaaaaaa taaagatgag gccattaaag caataaatgc aattactgcg
    12481 ggtggaatta aagggattga actaactttt acagttccga atgctgaaga aataatcaaa
    12541 gaaattgcta aaaaatatgc tgatcaaaaa gatgtaataa ttggagccgg tacagtttta
    12601 gatgcgttta ctgctcgtat tgcaattatg gctggagcag aatatatcgt aagtccatct
    12661 tttgatcgac aaacagcaga gatttgtaat ttatatcaaa ttccttattt accaggttgt
    12721 atgacaatta ctgagataaa ggaggcactt accgctggag tagaaatcat taaactattt
    12781 ccaggttctg cttttggtcc atctgttgta aaaacgtatc aagctccatt accatatgta
    12841 aatataatgc ctactggcgg agtaagtcta gataacttag aagagtggtt tgaagcaggc
    12901 gttgttgcag ttggtgtagg tgggaatttg ttggctcctg ttgaaaaagg tgattttgaa
    12961 gaagtaactc gaatagctag taaatatata gataagtttt atgagataaa gaattattag
    13021 gagaaaaaat aaataatact ataaaatgta aaaaataaat actaactctt tatacttcag
    13081 agtatagtac gtaagttggc agttcaacag aatgaaaacg cttaacaata ggaaggagcg
    13141 caaattatat ggaaagaaaa tttggtctaa gagacaaaat aggatatatg tttggagatt
    13201 ttgggaatga tttcactttc atttttgcta gttctttttt aatgattttt tatacaaaag
    13261 tattggggat aagcggagct accgtaggaa cattattttt agtagcaaga tttattgatg
    13321 cagttacaga cataacaatg gggcgtattg tggatggatc aaaaccatct aaaactggca
    13381 aatttaaacc gtggatttta cggatgagtg gtccagtcgc tttagcaagt tttttaatgt
    13441 atcaaacatc aatggcatca gcttctatga gtattaaagt tatatatatg tatgtaacat
    13501 atatactatg gggaagtatt ttttatacat ctattaatat tccttacggt tcaatggcat
    13561 cagctatcac ttctgttgca gaagaaagga cagcgttatc aacatttcgt agtataggag
    13621 ctaccttagc aggtatgttc attggcgtgt taacaccaat ttttatttat acaagagata
    13681 gttctggagc tcaaattgta cgtggtggat caacttttac aattgtggca ggagtatttt
    13741 cagtgtgtgc agttatttgt tacgtaatat gttataaatt aactgtagaa cgggttaaaa
    13801 gtgatgaatc gcagttaaaa gtttcattgt tgaaatcttt aaaaggatta gctaaaaata
    13861 gagcgttgct ttctataata gctgcagcta tttttttgtt gcttgctcag cttttaacaa
    13921 tgagtatgaa caattatgta tttccagatt attataataa tgcacaggga atagttttaa
    13981 tgaactttat caatcctatt ttgatgttgg tacttgtttc acctgtaagt ttaattttca
    14041 gtaaaaaata cggtaaaaaa gaattggcgt cagtagggat gttcttctct gctattgtat
    14101 atttactttt atttttgata aagcctagta atatgtatgt attcttagtt cttacttctg
    14161 ttggatatat ggggttaggt atttttaata cagtcatttg ggcgaatatt actgatgtaa
    14221 tcgatgatca agaagtgaaa tctggccaaa gagaagatgg aactatttat gcagtatatt
    14281 cttttgcccg aaaaattgga caagctttag ctggcggtgt cggaggttgg actctttcta
    14341 taatagggta tgattcgtta gcaaaagtac aaagtggaga agttttacaa aagctgtata
    14401 actcttcaac gttgattcca gcagtttgtt tctttgttgt aggattgatt ttatttttcg
    14461 tatatccatt atctaaagaa aaagtattag aaaattataa tttgctaaat caaaaacata
    14521 actaggagaa aaaacatgaa cagttggata ttttgtattg attcagatgg ttgtgtcatg
    14581 gatacaatga cttataaaca caatctcttt ttcgggccaa ttgctgcaga gatttttgat
    14641 gttcatcata gggaaaaatt cctacaggat tggaataata ttaatttata ttcagagact
    14701 cgtggaataa acagattttt aggtttagtt aaaacattag aaaaaaatga tgtaccatcg
    14761 attaatcgat taaagcactg ggtatctgta acaaaagaat tgtctaatcc atctttgaag
    14821 agggaaatag aatactctca gtcagaggat ttattaaaag ctttaaaatg gtcggaagaa
    14881 gtcaatcgac agattagtca agctaaagga ttagataaac cctttttaag tgctagagat
    14941 actataaaag ctctgaaaaa atatgggaag ataattattg taagctcagc taataaggaa
    15001 gctattcaag aagaatggga aagacatgaa ttattatcct tagttgatga actgtgttgt
    15061 caagataaag gaaaaaaaga agacattatt cgaagtgtaa ttgaaaatgg ttgtgatttg
    15121 gataagatat taatgattgg tgattctcct ggtgatctag aagctgcaaa taagaataaa
    15181 gttttctttt atcctatttt ggttaataaa gaaaaagaat catgggaaaa cttaagaact
    15241 gatattttat ttaaatgttt atcgggagag tatatagata ttgaacaaaa atatatagat
    15301 actttttgga aaaatcttac taataagtag aggagaaaga aaatgactat aaaagtagac
    15361 ttacgaaaaa aaccatatca tttagatgaa gatgctattt cttggattga agagacgatt
    15421 gatgctatga ctttagaaga aaaaataggt caattatttg ttaatatggg atctagtcgt
    15481 gataaaaatt acttaagtag tatggtcaat cagtaccata ttggagctgt acgatataat
    15541 ccaggaactg ctgatgaggt atatgatcaa aactatatcc tacaaacaga aagcaaaatt
    15601 cctttattaa ttgctgctaa tacagaagca ggtggtaacg gagcttgtac agatggtacc
    15661 gaagttggtg tagaagtaaa aattggtgca acaaatgatg cgaaatatgc ttatgaatta
    15721 gggagagttt ctggaattga agcatcggct attggatgta actggagttt tgctcctatt
    15781 gtagatatta atagaaattg gagaaatcct atcatttcta gtcgatcttt tggacaaagc
    15841 cctgataaag ttttagaact tagtttagct tatatgaaag gtattcaaga gagtggaatt
    15901 ttgcctgcag ctaaacattt ccctggtgac ggtattgacg aacgagatca acatctatct
    15961 tttagtatta atagtttaag tacagaagaa tgggataatt cttttggaaa agtatactca
    16021 ggcttaatag aagctggatt accttcaatt atggcaggtc atatccatat gccagcttat
    16081 tcaaaacatt ttaatcttga tctgaaagat gaagatttac ttcctgcaac tttatcaaaa
    16141 gaattaatca ctgacttact tagaggaaaa ctcgggttta atggattagt agttactgat
    16201 gctagtcata tgttaggaat gactggggca atgaaacggt cagaattatt gcccacttct
    16261 attgcggctg gatgtgatct attcctattt tttaatgatc cagatgaaga ctttaattat
    16321 atgatggaag gttattacaa aggaattatt tcagaagagc gtttacgtga tgcattaaga
    16381 agaattttag gcttaaaggc taaactaggt ttgcataaaa aagaaaagaa taaaattttg
    16441 ttaccgaaac aagaagctat ggaaaaaatt ggtttaattg aaaataaatc aatttttaaa
    16501 gaggtagctg atcaagcaat tacactagtt aaagataaac aagatatttg gcctattgta
    16561 ccagaaaaat ataaaaggat tttactggta gatgtacatg gggtaaaagg tgggtttggt
    16621 gaagtagttg ctggaaatag acctcaggcc attgatttat tgaaacagat tttgtcagca
    16681 aaaggatttg aagttgaaaa atacatttca cctgaagaag aattgaaagg attatcgcaa
    16741 gaagaaaaaa ttaaaaatat agctggaata tatgctgcta agagaccaat caaagattta
    16801 actgaaaaat atgacttgat tattaatata gctaatgtta acccaggtac agttcaaaga
    16861 attgtttggc cagcaagtaa aggtactccg gatattccat tttatattca tgaaattcca
    16921 acaatttttg tatctgtaca gtgtccgttt catttagctg atgtgcctca agtaaaaact
    16981 tatatcaata cttatgatgg aaaacaagaa actttagaaa tattagtgga caaactaatg
    17041 ggcctttctg aatttaaggg aataagtcca atagatgctt attgtgggtt aaaggataca
    17101 tttatttaat atttaaatta tataggagtg atttacatgt tatatcccat tatgacagag
    17161 acgaggcaag taattgattt aagcggtctt tggagattta agttagatac aaatgatgac
    17221 ttacaagaag aacttgcttt agaaccaatg gttgaaaaag agagttatcc aatttcagtt
    17281 ccttcttctt ataatgattt atttgaaaca gagaacatta gaaatcatgt tggatgggtg
    17341 tggtatgaaa aagaagtgat tcttcctaaa aaattattag aagaaagatt agttcttcgt
    17401 tttggatctg caacacatga agcaaaagta tttttgaatg gaaaattgat taatcaacac
    17461 aaaggagggt ttacgccatt tgaatcagaa attaatcagt ttataaaaga tggaagtaac
    17521 agaattacag tggctgtaaa caatgttatt gacgatagca ctttacctgt agggattttg
    17581 aaagaaacga tacaggaaga tggagacata aaaaaagaaa atttggttaa ttttgacttt
    17641 ttcaattatg caggcattaa tagacccgtt aaattgtata caactccgaa agactatata
    17701 aaggatgtca ctattaatac aaaaataatt gattcagatg caaatgtaga atttaatatc
    17761 gatacagaat ggaccggaga aattctaata gcaatctatg acgaagaagc tcaacttata
    17821 gcagaagcta aggggaaaga aggagtgatt caacttaaga acccaacact ttgggagcct
    17881 ctaaatgctt acttatatag tatgaaagtt agtttaatta acgaaaataa agtgattgat
    17941 acgtacacag aagaatttgg cattcgtagt attcaggttt cgaatgggaa gtttttgatt
    18001 aataataaac ctttttactt taagggattt gggaagcatg aggacacgta tattaatggt
    18061 cgtggtttga atgaagcagt aaatatcaaa gattttaatt taatgaaatg gatcggtgct
    18121 aattcattta ggacttcaca ttatccttat tcagaagaaa ttatgcgctt agcagatcga
    18181 gaaggtattg tagttattga tgaagtacct gcagtaggat tacatttgaa tttcatggct
    18241 actttattag atgacaacgt agaaaaacat gatacttgga aagaaatacg gacagaaaat
    18301 gctcacaaaa tggcgattaa ggaattgata gaacgcgaca agaaccatcc gagtgtcgta
    18361 atgtggtcta ttgcaaatga accagattcg gattctgaag gcgctaaaga atattttgaa
    18421 cctttagttg aattgactaa agaacttgac cctcagaaga gacctgtaac aatcgttact
    18481 tacttaaagt caacaccaga tatttgtaaa gtaggagata ttgtcgatgt tttatgttta
    18541 aatcgctatt atggttggta tgtttcaggt ggagacctaa aacatgcaaa atatctgttg
    18601 aaaaaagagc tagatggatg gatggaaaga tgtcccgata cgccaataat gtttacagaa
    18661 tatggagcag atacagtagc tggacttcat gatacagtac caacaatgtt tacagaagag
    18721 tatcaagtgg gttattacga agcaaatcac gaagtaatag ataaatgtga caattttgtt
    18781 ggtgaacaag tgtggaattt tgcagatttt gaaacaagtc aagggatttt aagagtgcaa
    18841 ggaaataaga aaggaatctt tactagagat agaaagccta aaatgattgc tcactatcta
    18901 aagaaacgat ggtcatcaat accaaatttc agttataaaa aataatttac cttttataag
    18961 gaatataaaa aaggagtggc tgcttaattg ccaactcctt ttttttataa aaagattttc
    19021 tgtttaaaga aggatatttt tttctagttt tttcgaaaaa aaggcgcttt ctgattgttg
    19081 tattagtgga aaggggaaaa aaggagcata tttatcatgc aacaaaatta caatgagaaa
    19141 tctatacaac atcaatttga tgcctactgt aaaaaagtat tacgaaacga aactaagtct
    19201 attcgaaaac gaaacattaa cataacagat aatgaggaac cattgacgaa ttcaaatgaa
    19261 ggtaagtact cccaaactca ttttgatgaa caagatgtct atttcttatt tggaatggaa
    19321 atattaattt cagatcaaaa gttatcggca gccattgatc atttatcgga aataagaaga
    19381 aaggttatcc tactttatta ttttgcaggt tttaatgata ctgaaatcgg taaaatcctc
    19441 aatatgagta caagtggtat ctggtatcaa cgaaagaagg cagttgaaca attaaaaatg
    19501 gggtatgact tatggtagac aaccccaaaa aacggcagta tccattagtt cctctatcca
    19561 ttatccaatc tgctctagaa gaagatccaa tttcaatcaa gatgatactc aaacattatc
    19621 atagttatat agctaagtta tgtttgacaa atggatttaa tgaggcgggg caatttatta
    19681 cctatgtaga tgagtatatg ctaagacagc tagaaattaa attgatagaa gcgatcttaa
    19741 aatttaaaat taactaacta aaaagtcccc tttccactta ttctgtttaa aagaatcttc
    19801 taatagttaa aggattagta gattctttta aacagaaaac ctattgatca ttgaaattta
    19861 aatacattct atcagtacgt taacacttca aggagcatat tttagttgcg ccatgatttc
    19921 ttactgtaaa gaaggagcga taaacattct aaaataattt ttatagtcaa taaaaagcga
    19981 tgatttgaag ttgggataat gatacttccg taaggctcgc cgtaaacttg gagagaggtg
    20041 aaattcctat gtgcttttga caaaagcagc tgattaagaa ttatttccat atgttgattt
    20101 caacattttt tctattattt ttaagaccaa attcagtgag gcaaatagca tcatgtaaat
    20161 ttagtttttc tattgaacgt tctccacttt ttagtttttt tatagtactc aaatttaatc
    20221 ctgtatgttt agagatctca tttgcagtat ggttttgtaa agtttctcgg attattgtta
    20281 tattagcaat ttcattacgc ataattatgt tcctttctta tcaaaaatat caatgactac
    20341 agtgggcttt tagtgaaaaa aaggggtact acagtctctt ttgaaaacaa aaaaatacag
    20401 tatatacgtt ttctaaatgc taagcaccaa cgtttacact gtatttaagg gtttggcacc
    20461 caatgggctg tgaggggctc gaacccgcga cccgatgatt aagagtcatc tgctctacca
    20521 actgagctaa cagcccaaat taatactttg ttattctaac acctaaaata aaaaagtgca
    20581 atacttatta tcagaattga gagtgaattt tatggatttt caagattatg aaaaaatatt
    20641 tgaaagttat ccatcgatat tgaaaattac tgatttaact aaaatgttaa atattggaaa
    20701 aaataaagct tataatttgc tgaaagatag atcaatagaa aatataaaaa ttggagcaag
    20761 gtacaaaata cctaaaaaag ctgtcattga gtatatattg aaaatacaag atgaaggtaa
    20821 aaatcatgct ataataaaat agtcaaaatt tttgccgata tttcagttta agaggagaga
    20881 ttgaattgaa attaagagca aacataattg aaaaaagagg aacattacaa gtagttgtat
    20941 cttataaaga ctctaacgga aaaattagac aaaagtggaa agatacaaaa ttaaaaaaga
    21001 aaggaaataa aaaacgagca gaaaaaatta gagatgaatt tttactagaa ttggaaagtc
    21061 aattagctat ggaatgtgaa tgtgctaatc caaatttatt gttttatgac tatttgctta
    21121 attatttgga agtatcaaaa aaacaagtag catttaacac atatgttggg tataagcatt
    21181 acgtccataa tcgaatttac aagttttttt atcctaaaaa aattaaacta aataggttaa
    21241 agccttatca tttacaagag ttttatcaat atatgttaaa tgataactgt acagcaaata
    21301 cggttattca ttatcacgca tttatacgca aagctcttca agaggctgtt attacagaat
    21361 tgataagtac aaatgtagca gataaagtaa gaaagccaaa aaagaaacaa tttgtttctc
    21421 aagtttataa tcaacaagag atcttgaaat tacttgacat tttgtcagaa gaaaaactct
    21481 atttggtagt tcttttaaca gcattttatg gattgcgaag aagtgaagtt ttaggattaa
    21541 aatggtctgc tattgatttt ttaaacaaaa aaatcatgat aaaccatgta atcatagaaa
    21601 atccagagaa tatgtcacag ttaataaaga aagatcaaac aaagaactct tccagctatc
    21661 gtacattacc tttggttgaa acgattgaac gagcattagt aaatcaagct aaatggcaac
    21721 aagataatcg tattctttta ggagatgatt atattttaga agataatgag tacgtattta
    21781 ctatggaaga tggacggttg atgaagcctc aatatgtaac gcatcgtttg agtaagttga
    21841 ttaagaaaaa tggattgaaa aaaattcgct ttcatgactt gcgtcattct aatgcttcaa
    21901 taatgttaga tagtggccag aatatgaaat ccattcaaga atggctggga catgcatcgt
    21961 attcgacaac tgctaattta tacacgcatt taaccgcagg agtaaaagat cgtgcagcag
    22021 aagcattaga aaacgcattt gggtttaaag aataaaaaaa caaaaaacaa tatctttggg
    22081 gtatcgcttc ttgtttttag aatttttttg ttggcaattt gttggcagta tgttggcaat
    22141 gaaaaaattc acaaactaaa aacctattta taaagcttta taaaactttt ttcacaaata
    22201 gagcatttgg ttaagagtca gctgctctac caactgagct aacggcccgt acaaaaagta
    22261 cttaataata gtagcactaa gaattttaaa atgcaagact acatttaaag aaaatcagaa
    22321 aaatctttac ttccttatag gaaatcattc atagattatt cacatttgct cgctattata
    22381 taagcatacc aacaaacaaa gttcttgcaa gagaacaaac cctaacactt ttttcatttt
    22441 ttactccttt cccgccgtct ccccggcggg tctcctgttc ttaacgataa aagacaagaa
    22501 ggcttagcct tcttgttttt tgttttcatg gaatcccgct aatcaaatca cctagttaaa
    22561 gcaactagat tgaatgttta tcataagtct gatatcatgt ttatgaaatg aaaaaaggag
    22621 cggttatgat ggaaaaaatg cgtcagcaat taatggagtg ggggaaggaa ctagaaacat
    22681 tccgtctgcc tcactgggaa gaactccctg acttagaact ttatatggac caagtcagaa
    22741 cgttagttga tcgttatctt tcacctgtga tacaaggaga aaagcatcca ttgcttacct
    22801 cttctatggt aaacaattac gttaaactag gtttgatacc agcaccagtt aaaaaaagat
    22861 ataacaaaga acacgtagca tttttacttg caatcactac gttgaaacaa gtattgacta
    22921 ttcctgaaat caaagaaggg atattgttcc aaggaaaaac agtaggaatt cgtgaagcct
    22981 ataatctatt ttgcgatgaa caagaagcag ctgtgtggat ggtcagccaa ttagcacaag
    23041 gtaaatctca tccgcaaaaa ttcgaccagg caacccctgt agaatatatt gctgtaagag
    23101 cggcaacttt atcgtttgca atgaaacttc ttgcggaaaa aacgattgtt ctagaaaccg
    23161 aatatttaaa agaggagaaa acaaatgaaa aacaataaaa tcgcaatttt agtagattca
    23221 ggtacggatg ttccagaaga attgataaaa aaatacgaga tgtatatgat cccattgaaa
    23281 attatttata aagatcaggt atatacagat aaaattgaca ttacccctga agagatctat
    23341 caacgtttgc cagaggaaat ccctggtaca tctttgccgg atggtgagtc gatcacagaa
    23401 atctttgagc aaatcaaaaa agacggttat gaaaaggtac ttgcggtaac aatttccagt
    23461 ggattgagcg ggacatataa tgtggttcgt ttaattgcac aacagcaaga agaattggaa
    23521 gcatatgtac tagatacgaa aaatattggt attggtgcag gtttttctgc gatccaagca
    23581 gcaaaatggc tggaagaagg agtagaatgg gatcaactga tcagtaatct gaatgagtta
    23641 gtcgaaagaa caaaagtttt ctttaatgtt gcgacattgg aatatcttca aaaaggtgga
    23701 cggatcggtt tagttgcttc tattgtagga accgctttaa agcttaaccc aatcatctca
    23761 tgtaatgaag aagggattta ttacacagta ggaaaagcta ggggacgtaa aaaaagtttg
    23821 gataaaacgg ttgcttacgt caaagaaaaa atcggtaatg caaaggtctt caacttagct
    23881 gttgcccatg gggatgcgaa agaagaagca attgaaatgg aagcgagact taaaagagag
    23941 ttcccacaag caaaccaaat ctactttggc cagatttctc cggcattagt agtccacact
    24001 ggtccgggat tattgggtgt cggtgtacaa gttttagaag gataaatcaa aaaaaggaaa
    24061 cactgaggtg aaaatcagtg ttttcctttt ttattgaagt tagaacaaaa aataataaag
    24121 tattttgcac tttcatttta attttctctt ttgttttata ggcaaagatg aaacaaaagc
    24181 agaaatacgg tataatgaga aggtatgaca ataatatata agaggtggct ctttatgaaa
    24241 aaagttttag ttgtcgatga tgagaagccg atttcggata tcgtcaaatt caatttagcc
    24301 aaagaaggct atgacgttta tactgcatat gatggggaag aagccttgga aaaagttgct
    24361 gaggtagaac ctgatttgat tttacttgat ttgatgctac ctaaaatgga cggcttagaa
    24421 gttgctcgtg aggtacgcaa aacttatgat atgccgatca ttatggtaac agccaaagat
    24481 tctgaaattg ataaagtcct aggcttagag ttaggcgccg atgattacgt gaccaagcca
    24541 ttttccaacc gagaattagt tgcacgtgtc aaagcaaatc ttcgtcgcgg tgccacggct
    24601 gcgaaggaac cagaagaggc agcacctgcg gaattgacga ttggtgattt gacgatccat
    24661 ccagaagctt acatggtgac gaaacgagga gaaacaatcg agttaacaca tcgtgaattc
    24721 gaactactct tttacttggc gaagcacctt ggacaagtga tgacaagaga acatttatta
    24781 caaacggttt ggggttatga ttattttgga gatgtacgaa cagtggatgt aactgtacgt
    24841 cgtttgcgtg aaaagatcga agataatcca agccatccaa attatttagt gacacgtcgt
    24901 ggtgtcggtt actatttgcg taacccagaa caggagtaat tggatgaaaa gaaaagtccg
    24961 cttttttcga tcagtcaatt ttaagatcgc tattaccttt attctgattt tattgatctc
    25021 gatcgaaatt attggtgcct actttatacg tggtttagaa cgttccacga tcaatacatt
    25081 tattaaagat atgaatcaaa cggtggaatc attagcaaca acaatcagcc cggaattgaa
    25141 tcgaaaagat aatgcagatg atgaagaggt aaatgcgaat atcaaaagat ttattgaaaa
    25201 cagtgcgact agcgatatca tcgaaatacg ggtcgttgac gaaaaaggga tcatccgagg
    25261 aacaaccgat gtcaatgaac aaagcgctgt gggaaagaaa aatgattatg ttgatatcaa
    25321 tgatttcacg actaaacgtt atgttgcttt agataacgac gataaacggg tgaatatcaa
    25381 tgttcaaccg atcctgtccc cgaccggcga tgccgttatc ggggctcttt acgtcaaaag
    25441 taatatcgaa caaaaatatt cggaaatcaa taacacagca gtcattttct ttactgcctc
    25501 actaattgcg gcatttattt caatgatcgt atctgtttta gttgctcgtt cgattacaca
    25561 gccgatcggt gaaatgagag aacaagccat tcgaatcgct agaggagact acagtcggaa
    25621 agtcaaagtg catggacaag atgaacttgg acagctggct gataccttca atcagttagc
    25681 tgaacgtatt gaagaagctc aagatacaat ggaagctgaa cgcaatcgtt tggatagcgt
    25741 gctatcccat atgacagacg gcgtcattgc gacagatcgc aggggtaaag tcattacgat
    25801 caatgatatg gctgtttcct tgctagatat caaaaacgag gaagcaatcg gacaatcgat
    25861 tttgacactt ttagatatcg aagaagaata tacgttgcgg aaattattag aagatccaaa
    25921 cgaaatggta cttgaccgtt caaccagtac attggaaagt gatcagatga tcttgcgagt
    25981 agacttttct atgatccgtc gcgaatcagg ttttatcagc ggattagtag ctgtcttgca
    26041 tgatgtgaca gaacaagaaa aaacagaacg ggaacgtcgt gaattcgtct ctaatgtttc
    26101 ccatgaactt cgtacaccgt tgacaagtat gcgtagttat atcgaagcat tgagcgaagg
    26161 tgcgtggaaa gacgaggaag tagcaccaaa ttttttgaag gtaacgctag atgaaacaga
    26221 ccgaatgatt cgaatgatca atgacctctt aaatctatct cgcatggata caggtaatac
    26281 ccagcttcaa ttagaatatg tcaatttcaa cgagttagtg aattttgttc ttgaccggtt
    26341 tgatatgatg gtgggaaatc aggaaaaaaa ttataagatc cgtcgtgaat ttacgcaaag
    26401 agatctttgg gtcgaagtag atacagataa aatcatccaa gtagtcgata atatcatgaa
    26461 taacgcgatc aagtattcac cagatggggg aacgattact tgtcgcttat tagaaacgca
    26521 taataacgtg attttaagta tcaccgatca aggattgggg attcctaaaa aagatctgaa
    26581 ccgagtgttc gagcggttct atcgagtaga taaagcacgt gcaagagcac aaggtgggac
    26641 aggacttgga ttagctattt ctagagaagt gattaaggcg catcgaggcg ctatttgggc
    26701 agagagcaag gaaggaaaag ggtcgacttt ttatatctcg ctgccatatg aaccttatga
    26761 ggaggaatgg tgggaatgag aatctctgaa aaagtcgttc gtataggact cattttgatg
    26821 gttgcgttaa gcatatactt ctcttatgca atctggctga gcccagcagg aaaaagttca
    26881 atcaacgtag atgagacaaa cactcaaatg atcgagtctc aaagcaatcg aaaaacttcc
    26941 gaagtctttc ttccgcttca tgttacatgg caccattcgg gaatgatcca agaaaccaac
    27001 agtgaaaatc tcgtgtaccg attgcaattg atcatcgata aagcaagatt tggaaagctt
    27061 tctgaagtag tttcaggaga caaatcacag tttgaacagg tgaaaaatct gaactctggt
    27121 tttgagttgg cctataatgc gccttttttg ctaagagaat acaaggaagc atttgattta
    27181 gatttagatg tgtcgaactt ggcaaaagaa agcgaaaata tgtattttac tagaatacaa
    27241 ttcgatcaag gaaataaaaa aatccgattt ttgaattatt cgaaccatct cgtatatgaa
    27301 gcaaatattt cgttgaattg gacagatatc aataaagaat taacagcaaa agacacaaaa
    27361 tggacagaaa tgacaacaga accatcgacg atcgacacgc aatatgctac gaaacattcg
    27421 atcaaactga agaagtatag ttatatcctg tcacagcaac cctatacact ttttagaaat
    27481 gcttttttcc agaaaccaga tagcgtgaaa aataatgatg aaagcaatga attgattttt
    27541 tacggtggga atgaaacaat gtctatccat tcggaaacgc aagtagttga ttttcgtgga
    27601 gagctgccag agaaaaaaga aaatgctagt gtattttctg aaagtttccc ctatattagc
    27661 aaattaggca gttctttagg gaacctcaga tattttgacc gcagtgacaa tacgatcgat
    27721 tatcggatat tcgtggaagg cttcccggtt tttgggacag acagcagagg acagattggt
    27781 gtggaagttg gcggtgaaac gaatggttct gtgcaagtga atatccaaac cagtttgaat
    27841 acgattcaag tgccgatacc ttcagacgaa gaagtagaac ttgcaagtac gaatgaaatc
    27901 atccagcagt taaaagctaa gggagcagat agcaacaaga ttggttcagt gatcgttggc
    27961 tatacatggc aaaatgtcaa agaaacgaat cgtgtcgttg atctcttacc tgaatggtat
    28021 gtgaaatata aagataactg gtattcagct aacgaccttc tggaaaaatt acctggattg
    28081 gaggcgaatt aattggattt caaaaaaatc gaatggatat ttttcgtcgc ttttttaggg
    28141 ttgaatgtgt ttttattcag cagttatcat gacgcggtat accaagaaaa caatgtcatt
    28201 cgctctaatc agactgaatc aatccaacaa cgtctggcta gcgacgatat cacttacacg
    28261 gggaagattt cgtctgagaa caagcaaggc tattatctta gcgcggagca gaccaatctt
    28321 agcacttcat tagcgaatta tcgcaaggct ggtaaaagcg gcttgctggc aagtggtgtg
    28381 tttttagacg gtgatgtttt gacgaaatct ttatccagtt cagatgctga aaagaacgtg
    28441 atcgatccag ataaaattag tagttccttg gaagattttt tatctaacaa agacaatatt
    28501 ttattcggta atgattatgt gtacacgaaa aacttttctc atttagaaga agaaccacta
    28561 gaaatccaag caagccaaga gtatgaaggc atcccattca atgatgatac agcaaaaatc
    28621 gttctttctt tagaaaaaaa cgataatgat tatagcatca cgaaatacat gcagacgcat
    28681 ttatctgata tcgagcagct gcgcgaaaag acggaactgt atacggaaga ggaagcaatc
    28741 aatacattgt acatcaataa caaaatacca agaggatcaa aaattttatg gcgtcagcta
    28801 gcttattcct gtattctgaa agtccgggaa aaaaatgtgt atgttcctgt ttggcatgta
    28861 gcgatcgaaa caagcgataa agtggtccaa attgagagcg tcaacgcttt cagcaatacg
    28921 atcgtgacaa acaatactgt accaaaagtg gaagatcaat aaaaaagcga gtataatgaa
    28981 aaaagtttac aaagtaagaa gggaagtagg agtcaaatgg ctgaagaaaa accagctttt
    29041 aaaatcagta tcctagcaag tggaagttcg ggtaattcac tttatatcga aagtgaaaaa
    29101 aaacggcttt tggtcgatgc aggacttagc ggtaaaaaaa tcacttcctt gatggctcaa
    29161 attgatcgga caccggacca attagatgga atacttgtga cgcatgaaca tcgagatcat
    29221 atacatggcg tgggtgtctt ggctcgtaag tatcatttgg atgtttttgc caatcagcag
    29281 acttgggaag cgatggaacc actgattgga aatgtaccag tggaacaaaa acatctattt
    29341 gagatgggga aagtcagaac attcggcgat atcgatattg agagtttcgg cgtctcccat
    29401 gacgcagcgg ctccgcaatt ttatcgtttc cataaagaaa atcggtcatt cgtcatgttg
    29461 acggacaccg gatattgcag tgattatatg cgaggtgtca ttgaaaatgc cgatggttat
    29521 ttgatggaaa gtaatcatga tcttgagatg ttacgcatgg gaccttatcc gtggaattta
    29581 aaacagcgta ttttaggaga tagaggacat ctttccaacg aagacggtgc acttgtcatg
    29641 actgatgtga tcggtaatcg gaccaaacga atctatcttg gccatcttag caaggaaaac
    29701 aatatgaaag aacttgctca tttaacaatg gaaaatgttt tgaaagaaaa agattttggc
    29761 gttggacatg attttgaaat ctatgataca gatccagatg aagcaactga gctattttcg
    29821 atttaaacag ttcaatcaat aaaaatataa aaagcagaag ataaaatcaa tcagggttgg
    29881 ttttatcttc tatttttatt tttttagcat attaaaaaaa taaccgtatt atcgactgac
    29941 tacactgatg tgctgctttt catgtttctc atccagtatc tcatctacta atgcaatcgc
    30001 gtaatctgcc atgctgatcg tactgtcgct agcagcgttt ctttgtaaat aatcatcact
    30061 taacaaataa gtacctgtac gtttgccatg tggatcaaat aaagaagctg gactgatata
    30121 agtccaatgt agatttttag attgtttcaa ttttaacaat ccttcgtaca tattgaaagc
    30181 tgttggatag aagggtgcat ttttaggtac ttggtcaatc atacgagtcg tcttttctgt
    30241 atctaagaat aacgaagagg cacccccaac gacaattaag cgtgtatcgg ttccttcaag
    30301 catctcaatc aaatgggaca aagaagtttg atgcatttct tcttttcctt tgggagcgtt
    30361 gaaagcatca acgacaacat caaaagatgc aagatcagta gttgtcaact caaataaatc
    30421 cttttgtata aacggaacat ctgatggcaa tttttctgga tgacgcacga ttgccgtcac
    30481 gtctaaatct ctggacaatg cttcgtctaa aataaatgaa cctgcatggc cagtagctcc
    30541 aatgagtgca attttcataa aaaacctcct tttttattcc tgtccattgt aagcagttgc
    30601 tgaatattcg tcaaagaatc cgatttttga aaagctcagt gcgcattcct ttcatggttc
    30661 gtgatcattg ggtatagtta aattaagaaa actgattgat aaaaatagaa gttgaaggag
    30721 ataagaaatg aaagagtggt tagatcgtgc ctttttacca atgataacat ctgttgtgaa
    30781 tcttagaccc ttaactgctt tgcgtaatgg aatgacttac acgatcccaa taattattgt
    30841 tggctcgctg tttttcttat ttgctcattt tcctgtggat tacatcgctc agtggataga
    30901 caaaacagga ttttctatgt attttacaca agtataccag gctacatttg atgtgatagc
    30961 cttttggatc gtttttgcca ttgcatacgc ttatgcaaaa gaagaagaca tcgatcgctt
    31021 accagtcggc ataacagcct tgtctgctta tctactgctt attcagccac taaatactca
    31081 gatagatact gtctggacag gtgctgaagg gatactagga gcagttacaa ctgggctcat
    31141 catcggatgg ggctatgcac gtttagtaag acttgcgaaa gggtggaagc aaagagagaa
    31201 gatacctgaa aatgtttggc agtcttttac ttcattgatt ccaatcattg ttatagtcac
    31261 ttttacgctt atcggttcag cggtttttca acatttgttc agaatgactg ttgttcaagg
    31321 agtatggaca tatgtccagc ttcccattca atcgttcata gacacgttgt ttggtgtgct
    31381 tttattaggt ttttttgttc cgtttttatg gttatttggt cttcatggat ctgcgatggt
    31441 caatgggttg gttagtcccg tcttgcaagc gaattcatta gcaaatgcag aaatcttagc
    31501 ctcaggacaa gagttgacgg tggcaaatgg cggacatatc gttactcagc aatttctcga
    31561 tcagttcatg acggttacgg gtgctggatt gacgttaggg gctgtatttt ttatgacggt
    31621 ttttgcgaaa tcgaagaaat acagagaatt aggaaatctt gctcttttac cagccttttt
    31681 caacatcaat gaatcaatta ttttttccac accgatcgtg atgaacccga tgatggctgt
    31741 tccttttatt ttcgcaccgg tcgtttcagg attgattacc tattccgctt tatattttgg
    31801 attcgttcct ttatttacag cagtccaagt cccttggaca acaccgccga tcctctcggg
    31861 tttcttagta ggcggagtgc ctgcagcgat tttacaaggt atcgtgttgt caattagttt
    31921 tttcatttat ttcccatttt tgaaaaaaat agattctgca aactgcaaaa aggaaagaca
    31981 gacaaaaaat gatggagcag cagaaaagat gaacgactaa aaaacaaaaa tactcctgtc
    32041 gctttagatt gggaaaatca ccaatctagg atgacaggag tatttatagt catgtttata
    32101 ctagcccttt ttggacagct aattgaacaa gaacaactaa tgtgttcatt cctacgtgtg
    32161 tgatcatcga tgtccagatc cgaccagttg ctttgtactc taggctgaag aagaaaccta
    32221 ggaaaaagta gattagcaga tgaccatcat tatgagcaaa cgcaaacagc aaagagctga
    32281 tcactgctcc gacccaaaaa tttgaatatc gactgatgat ccccagtata ctgcggcgga
    32341 atacgaactc ttccatgatg ggtccagcaa cagttgtcgc caaaataaaa gcgggtgctt
    32401 ccatgatcat ctggatgata ttttgtgtat tctcggaagg ggttgctttg ccaaacaata
    32461 cagattctaa catgacagcg atgctttgca gaatcaatgc gacaaagatt ccaaccaatc
    32521 cataaataac aatctttgga gcagaagctt tttttaccgc tgattcgatc gctaagggtt
    32581 cacgttgctt aaaataaaaa atagctagga caacggcgcc tagtatatac atgactgcag
    32641 ttgctgtgat caattcagac gtagatttta caagtcctat cgaagctagg atcaagggtg
    32701 agataaaaat tagaccatag caaaaaatgc tgaggaagct atattttttt aaagacatgt
    32761 aattcctcct tttaagggat aacaagcatg caaggcaagc ttatatcgat tgatttcaat
    32821 tatatcataa tttcaataag cagctgtcag aataaaattt cttttctttg atttaaaagc
    32881 ttgcaaaaaa gagggagaat gagtattatt aaaaatgtag gttagcactc gaaggttaag
    32941 agtgctaaca gattaaaaaa agagttatcg gagggattta tcgtgttaaa accattaggt
    33001 gatcgcgtca ttatcgaagt cgcaaaagaa gaagaaaaaa ctgtcggagg aatcgtttta
    33061 gcttctgctg ctaaagagaa accccaaact gggacagttg ttgcagtcgg tgaaggccgt
    33121 ttgttagaaa acggtgaaaa agttcctgcc gctgtaaaag caggcgacca agttatgttc
    33181 gaaaaatacg ccggtacaga agtaaaatac gaaggaaaag aatatctgat cgtcgcagga
    33241 aaagatatca tggcgattgt tgagtaaatt tggaagataa aaaaataatc aaaaataatc
    33301 aatgaggtga atcaatcatg gcaaaagaat tgaaatttgc agaagacgca cgcgcagcta
    33361 tgttacgcgg ggtagataaa ttagcagata cagtgaaagt aacattagga cctaaaggcc
    33421 gaaatgtcgt actagaaaaa tcttacggct ctccattgat cacaaatgat ggtgtgacga
    33481 tcgcaaaaga aatcgaatta gaagatcatt ttgaaaacat gggtgcaaaa cttgtatcag
    33541 aagtagcatc aaaaacaaat gatattgcag gagacggtac aacgacagca actgttctga
    33601 cacaagcaat cgttcgcgaa ggtttaaaaa acgtaactgc aggtgctaat cctctaggca
    33661 ttcgtcgcgg gatcgaattg gcaacaaaag cagcagtcga agaattgcat aatatctcaa
    33721 ctgttgttga ttcaaaagaa gccattgcac aagtagcagc tgtttcttcg ggttctgata
    33781 aagttggaca tttgattgct gatgcaatgg aaaaagttgg aaacgacggt gtcatcacaa
    33841 tcgaagaatc aaaagggatc gaaacagaac tagatgttgt agaaggtatg cagtttgatc
    33901 gtggttactt atcacaatat atggtaacag ataacgacaa aatggaagct gttctagaaa
    33961 acccatatat cttgatcaca gacaaaaaaa tctctaacat ccaagatatt ctgccattgt
    34021 tagaacaaat cttgcaacaa tcacgtccat tgttgattat tgctgacgat gtagatggtg
    34081 aagcattgcc aactcttgta ttgaacaaaa tccgtggaac attcaacgta gtagctgtaa
    34141 aagctcctgg tttcggcgat cgtcgtaaag caatgcttga agatatcgct attttgacag
    34201 gtggtacagt aattactgac gatctaggtt tagaattgaa agatgcaaca atcgaaaact
    34261 taggaaatgc ttctaaagtt gttgtagata aagacaatac aacgatcgta gaaggttctg
    34321 gagaaaaaga agcaatcgaa gctcgtgtgc aattgatcaa aaaccaaatt gctgaaacaa
    34381 cttctgattt tgaccgcgaa aaattacaag aacgcttagc taaattagca ggtggtgtag
    34441 ctgttgttaa agttggtgca gctactgaaa cagaattgaa agaattaaaa ttacgcattg
    34501 aagatgcatt gaatgctaca cgtgccgcag tagaagaagg aatggtctca ggtggtggta
    34561 cagctctagt gaacgttatc agcaaagttt ctgctgtaga agcagaagga gacgtggcaa
    34621 caggtatcaa gatcgttgtt cgtgctttag aagaaccaat tcgtcaaatc gctgaaaacg
    34681 ctggttatga aggatcagtg attgttgata aattgaagaa cgtggaacta ggcactggtt
    34741 tcaatgctgc tactggagaa tgggtaaaca tggttgaagc aggtatcgta gacccaacta
    34801 aagtaacacg ttctgccttg caaaatgccg cttccgtttc tgccttgtta ttaacgacag
    34861 aagcagtcgt tgcagataaa ccagaaccag cagcgccagc agctcctgca atggatccat
    34921 caatgatggg cggtatgatg tagtaattaa gaaagaaccc tattttaaca gggttctttc
    34981 ttcttttttt gttttatttt aagcaaaagg ggcaaagaag gggcaaaaat atcaaaggct
    35041 atttagttta tcaattactt gttgtttggc tctttttgtt acatggttat aaatagacag
    35101 agttgtgttt gcatcagaat gccctacacg ctccataatg gctttaagag gtacgcctaa
    35161 ttcagataat agtgaaacgt ggctatgtct gaatatatgt gaggatagac ttttttctaa
    35221 ttccaactct tcctctactt tatggagtat agcgttaaat gagtgcagcg caagcggagt
    35281 gccacttgta gatataaata tatattgatc gggatctgtg ggtctacctg caagaatatt
    35341 gtcagctatc acactttcaa tcaattcttt tgcacgattg ggtaattgca cttcacgttg
    35401 cgaataagta ttttttggag ttgtttttat agcattatcc attttcacag atgtgtaatc
    35461 taaggtccca ttaatggaaa ttttcccatc ctcatagtcc ttcatttgca aagctagcaa
    35521 ttccccatat ctcaaaccag ttaaatatag aaactcagct attatgccgt gtagttttct
    35581 gcgaggattg gagtatagct gtttcagtat ttgatcaatt tcttctttct ccagatattt
    35641 tttattcata gaaagccttc ttttttcttc ttccactttt ttaggatgga ttttaactgc
    35701 tagcgcaggg tttctttgaa tgtattttct atcgattgca tagtttagca taacagatag
    35761 agttgttttt gtttgttttg tgtagttcag tgagaggtca ccaaacgtat acatatcttc
    35821 aattatctta ttaatgagtg tctcatcaat gtttctaacg atcgtatcat cgcttatgtg
    35881 cttagaaaca tgtttcatca tcattggaac cttcaaatag ctagtacgtt taacatgctg
    35941 cttataatat tcataccatt ctttatacag ctcaccaaaa gtgatatctg atttattgta
    36001 atcttcgagt gcttctttaa tttttttatc tagaattttc tgagcttttt tccacgcttg
    36061 tggtgaatta cttgtaagtg ttgtagattt ttttcgtgtt ttttctgtat aaggatctac
    36121 atatctttca ataaacttga attggccatc tttggtctgt tcaacccaca cttttaacat
    36181 ctcctatcat ttgctataat aggcataaca aatagaccta tataggtttg ttttctaaaa
    36241 gcacgctctt actctggacg gtggggcgtg ttttttattt attatacagt tgctttaaaa
    36301 atagctgctt tttcaaatga tactaaagga gagaagtgga tttctatttc tcccttttgg
    36361 tttaacgtga aatgagcagt tacatccatt tgttttcctg gagcaacaga tcccattgta
    36421 ttttcgttgg catatgtctc tgattttttg tcatctggtc catatacttc cacatctgta
    36481 cctacaggga tatctgaatc gccatcattt tttacggtat aagtaatttt tactacttgt
    36541 gcaggttggt tttcttcaaa ttgatttctt tcatcagtta gttctacact atttagtgta
    36601 tattcagcat caccaacttt tacagtgtca ccaatcttat agaaggtatc gcttttttct
    36661 tctttagaag atgaagcaga ggttgaagat ttagttactt tttcgccacc attatcactt
    36721 gctttattac tatctgatcc accatttaaa gcagaaccaa taattataat taaaattact
    36781 gctaataccc aaaaccatac ttttttgtaa aaaggtttac taactttata catttttccg
    36841 tcttgaccca taactttttt tgccatttaa atattcctca cttcttgtta taatatattt
    36901 gcgatctcag aaatgaggta tgagtccgtg tgccagcacg ggctttttta atgttttgct
    36961 gaaatcggtt tttttaataa ttcttgatgt attcatacat attgccttgc gtaagaatat
    37021 tttttcttaa aatagcattg gcagacagca taacaagtgt atctgagctt attctgaata
    37081 gaataatact ccataaattt ttcgagatta aactgagatt catcagtcag ttcgttctca
    37141 atatagatat ttaataaaat tagaatagct attttatctg cttctgtttc aaattttgag
    37201 tgaaaagttg tagaagtatc gtacaaaact gaatattcaa aatgagaagc ggtgaaatgc
    37261 gcaagttcat gagataaatg aaaggcttct gcagtttcgc catatagatt ttcattcaaa
    37321 aaaatgattc taggcttcgg ataatagaag ccaggctctt tcatttccat gtaaactact
    37381 ttcaaattgt attcgctcaa catttctttc aatttcaaat acatacaacc catcactcca
    37441 actattcatt ttcctctaaa gctttagcaa ttgcaatcgc tttacgcatt gtctccttag
    37501 atatttcttt tccatcaaaa gaaaaaacag tatcgttttc tgataaatcc acatgtttag
    37561 gggtttctct ttcttctcta cctagaaggt agtctacaga gactccaaag taatcagcta
    37621 accgagctaa ctcaatagaa ttaggagttt gtcttttcca acttcctaaa tatccattcg
    37681 aatacccgaa attcatttcg agctgtctaa tggataatcc tttttctttt gctaactcct
    37741 ttattatttc gtaagtattc attgataaat caacctttct gaatgcttac aaaaaaagtt
    37801 tagataaata atgtaaaaat agttgactta tatacattat taatctatac tatgttttgt
    37861 aaacaagttt aacaactaaa aagacaacaa aaaatactat tgattaataa atgccaaccg
    37921 ccaagaaagc tttttaaatc aaagtttata tgtcttattt aactatgcat ttagtataga
    37981 ttattaatct aataaagtca acgcagtttt ttaaaaagtt gttaaatttg tttacgaata
    38041 taaaagaaag gagagaagaa tatggaaaaa acagtctcgg caaaaatcga agatttgaaa
    38101 ttagacatac tgaaacaagc aaaagtggcg atggaacacg cggtagataa agaagactct
    38161 gccatggttg cagccatagc agagatttta gctcacgttt agtcatcgtt tttacggtct
    38221 tctggaagct gaccatagat aacagcataa tgaatatcta aataagcttg aacaatttct
    38281 tttggcgaaa ttgattcgcc tttagtagat acttgtgatt cgtgatttag ccatgcaaca
    38341 actaaatccg cagcgatttc aaacggcgtt acactttgtg tcagttgcca taaagaattt
    38401 cacaatatgt atggtcgtgg taataatacc aaagagcaat ttgaggaatt tatggaaatt
    38461 aaaaaataca aaaaatcatt tattaaagga ggctaaacaa tgaacaagga aatgagccaa
    38521 acaatcaagg tacaaaaaat gatagatgat ctaacacatg gaattgatag ccaagccgat
    38581 aaaatcatca aggaattgca aggacaaaaa gttaaggatg caaaaatgct tctcaagact
    38641 atcaactttg aaatgaatcc aacaaaaaga aagctcgccg atgtgttgga agaaaagttg
    38701 gcttccgcta tcaacgagca agaattacta tttgaaaccg atacttttaa cagctgatgt
    38761 tttatagatg acagtttcat ccgaatctat atcatcgccg agagaaaaga atgttgcttt
    38821 tgacaaaaag ccgataagac ctacaagcgg tcttgatgtt gcaagagtag ataccccaat
    38881 atcaccttgc aaggtattca cgtaaatttg agaagaatag aacattccat ctttttcttc
    38941 gatatttgac gcatcatcgt atccaacgac caaagtatcg gcaaatattt tgattttttc
    39001 tccatcaagc atagtaatta caaattctgg tttcataaat tttcacctcc ttaacaatta
    39061 tttcagcctg tcacactgat aaggaaatta taccaaagaa aggaatgaga aatatgaata
    39121 caccacaaat ttttaatttc gagcaaaacg aagttcgaac tattttagta aatgatgaac
    39181 catattttgt aggaaaagat gtagcagacg ttctaggata ttcgaatccg caaaaagcta
    39241 ttcgagatca tgtggacttg gaggataaga cgcagaacga ttcgttcacc gtcaatggaa
    39301 cagcagttgt tttgattaac gagtcaggcc tttacagttt aatcttaaaa tcaaaacttc
    39361 cctctgccaa aaaatttaaa cgttgggtaa cgagtgaagt ccttccaaca attagaaaaa
    39421 caggtagcta ttcaaacgta cctcaaagtt ttgcacaagc attgcgttta gcagcggatt
    39481 tagaagaaaa gaaccaatta ctcgaacaac aaattgccga gtacgaacca aagattagct
    39541 acttagatac gattctttca tcgacagata cggtagctac ttctcaaatt gcagctgatt
    39601 acggaatgtc ggcaattgct ctaaacaaat tgctaaacga gttaggtgtt cagcataaag
    39661 ttagcggaca atggatactt taccgaaaac atatgaacca aggatacaca aaatcgcaca
    39721 caagtgagat accgaaagcc gatggcggca ctaaagttgt aatgaatacc aaatggacac
    39781 agaaagggcg agtgtttatt tacaacttat taatcgcaga gggctattac cctcaaatgg
    39841 atttagagga aattggttag aaaggagttt tagtatgact gacattgcag aaatcactca
    39901 acgagataga gaaaaaatca aagaatatgt cgaaagttca aagttcttaa cttacaccat
    39961 gcttgctgaa agatttggaa ttagcaaaag ctacttatct ttaattttaa acggtaaaaa
    40021 gacttctgca gaagcaaaca gaattataga ttcgattatc actatgtacg aattgtagag
    40081 ggaggtaaag tcgttggaag ataaaatcat tcttacaaaa tcagagcttc aagaaatgtt
    40141 aaacatcgag tatggaacga taatttttag gagggataat aagtgaaaaa accaacactt
    40201 tcagagctga tagaagctgc tgagaaggca gtaaaaccag acgactggta ccgacaaagt
    40261 ttaatcttgg agaagttcca cggcatgtca aaaactactt tagttgaata ctgcaaggaa
    40321 atggaaacaa ttcctgaatt tgcagaagga atacttcgtc caggtcactc aaccacattt
    40381 attcattacc atacttttat ttggttttta aagtggaaag acgcaaacaa atatcgtgta
    40441 aaaagtttat cgccttcaga tgttttgaag gaggcaagtt aatgggcaaa ttcaacagag
    40501 cactagtatt cagcgcaccg ctaatcatct acgctttagg cctctgggga agcagacaag
    40561 cgttgatagg gacaatcgtt tacatggttt ggatttttat ggggcttgat gaagctgagc
    40621 acagagcgaa aaaagccagt cgggagggac tgactaaaat atgatttttt tatttttgtt
    40681 cctttgggct gctattgcgt acttcatcca gctccttgtt gtcaaagttg ctcttatttc
    40741 ttttaggaag aacaaaagaa aatagcgcta taaatgaaag ataagtagcc gctacattaa
    40801 actgttcttt tgaaaaagtt acagtcatcg gaatgataat tgatattgag gaagtaatct
    40861 taattaaaaa tatagcaata ttttcaacat cgcgttggtt tttatttaac attgtccaac
    40921 atgttttagc aacaataaaa ataattacac atgatattag cacacagact gaaaaaagtt
    40981 ttagaaatgc gatgtagtca gtgttattga actccttcaa aatactattt ttattcaatg
    41041 taataaaagg aattttttta attgaagaca ggaaagataa ggtaataaac agaattatat
    41101 atatggttaa agtcgcagta agaatgtaat tgagcttttt cctcatagaa tcacctcact
    41161 ttctaacgaa aattatacca aaaaggagag aagaaataat gcaagaatta gtaattttga
    41221 aaaataaaga agctgtaact acgagcttac aagtggcaga aagtttcaaa aagaaacata
    41281 agcatgtact agaagcaatt gaatcaataa aaagatcggt cgaaaattcg gccaatgttg
    41341 aagatgggtc caattttgga cagatgtttg tggaagggaa cgagccagac tcatacggaa
    41401 gaagtcggag agtttatttc atgaatagag acggattttc cttgctagct atgggattca
    41461 ctggaagtaa agcaataaat ttcaaactaa aatttattga agctttcaac gaaatggaag
    41521 atgttattcg gaagaatact gttcctcaaa caattgaaga catgatgatc tatcaattag
    41581 aagaaatgaa agatgttaaa aaagatgttt ccatgcttaa agatactatg cgaattagcg
    41641 gacaacaaga gtttgaaatt aagcaaaaag gaaatatgaa agttatggaa gttctagggg
    41701 gaaaagaaag ccgagcttat gaagaaatca gcaaaaaagt attctcaaaa ttttggtctg
    41761 aatttaaacg taccttttca atcccaagat atggcgagtt acctcgtaag agattcgatg
    41821 atgctgtttc atttattgaa atgtggttac cagaaactgc gatccgcatg gaaatcgatc
    41881 aactgaacag acaacaaaga cttttcggtg atgaaaatga atagagctga agcgctaaga
    41941 atagggacgg taattgctaa tcgctggtgg agacacaata aaccaaacat cctaagccaa
    42001 caacatatcg aaaagcaaaa agcatggcaa caaataaaaa agtgactcag ccggcaagca
    42061 tagagtcaca aaacatatct aaggagaatt ttagcatatg aataaagaac tttccacttt
    42121 agatcaatat ttgactgatt ctgaatgggg caagtcgaat atcaaggaaa caaataatcg
    42181 aaaaatcaga cgaaatcttt tgacgaacga agaactatca tgtgatcaag atgacttggg
    42241 aaattttgtg agtatttggg atcatgttta ccttatccat ctatcaaaac attcaaataa
    42301 acctgaatac atctatgtca tcgaagatgg cttgattgat gcgctagaag agtacaacag
    42361 agataacttg attgatatct cttattacgg atcaggtaag aaatacattg ctgaaatgga
    42421 ggcagaattt gatgagtgaa atcaaaggga caacgaactt tgaaaaactt tttagtcgta
    42481 agttaaataa aattctcaag aaaaaaggaa attttgatta tttatcttgg gctcacgcgt
    42541 gggagattat gaaaaagaat gatccacagg caacggtaac tattaatgag tataaacact
    42601 acagggttgt ttctggaact catcaagact ttcttgttga ggaatataaa ccttttctta
    42661 tggatgaaac agggacttat gtatctgtct cagtaacggt taaaggacat acggaaacag
    42721 a
//
LOCUS       DAJSBN010000022        41006 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJSBN010000022 DAJSBN010000000
VERSION     DAJSBN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.022"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            259..527
                     /locus_tag="ONT86_001297"
                     /pseudo
     CDS             259..527
                     /locus_tag="ONT86_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            1199..4600
                     /locus_tag="ONT86_001298"
     CDS             1199..4600
                     /locus_tag="ONT86_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2352102.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIE
                     RSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADG
                     KLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIR
                     GNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(5024..5194)
                     /locus_tag="ONT86_001299"
     CDS             complement(5024..5194)
                     /locus_tag="ONT86_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2352103.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(5388..5567)
                     /locus_tag="ONT86_001300"
     CDS             complement(5388..5567)
                     /locus_tag="ONT86_001300"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352104.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            5954..7066
                     /locus_tag="ONT86_001301"
     CDS             5954..7066
                     /locus_tag="ONT86_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2352105.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(7144..7437)
                     /locus_tag="ONT86_001302"
     CDS             complement(7144..7437)
                     /locus_tag="ONT86_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR2352106.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            7605..7919
                     /locus_tag="ONT86_001303"
     CDS             7605..7919
                     /locus_tag="ONT86_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCR2352107.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            7940..9919
                     /locus_tag="ONT86_001304"
     CDS             7940..9919
                     /locus_tag="ONT86_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2352108.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            9932..10258
                     /locus_tag="ONT86_001305"
     CDS             9932..10258
                     /locus_tag="ONT86_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCR2352109.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            10274..10792
                     /locus_tag="ONT86_001306"
     CDS             10274..10792
                     /locus_tag="ONT86_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352110.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            10792..12144
                     /gene="celB"
                     /locus_tag="ONT86_001307"
     CDS             10792..12144
                     /gene="celB"
                     /locus_tag="ONT86_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR2352111.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(12228..13733)
                     /locus_tag="ONT86_001308"
     CDS             complement(12228..13733)
                     /locus_tag="ONT86_001308"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCR2352112.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(13825..14415)
                     /locus_tag="ONT86_001309"
     CDS             complement(13825..14415)
                     /locus_tag="ONT86_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2352113.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            14594..16240
                     /locus_tag="ONT86_001310"
     CDS             14594..16240
                     /locus_tag="ONT86_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2352114.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            16289..17008
                     /locus_tag="ONT86_001311"
     CDS             16289..17008
                     /locus_tag="ONT86_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR2352115.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            17487..18434
                     /locus_tag="ONT86_001312"
     CDS             17487..18434
                     /locus_tag="ONT86_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2352116.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            18446..19366
                     /locus_tag="ONT86_001313"
     CDS             18446..19366
                     /locus_tag="ONT86_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2352117.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            19407..20864
                     /locus_tag="ONT86_001314"
     CDS             19407..20864
                     /locus_tag="ONT86_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2352118.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            21209..21823
                     /locus_tag="ONT86_001315"
     CDS             21209..21823
                     /locus_tag="ONT86_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2352119.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            21816..23540
                     /locus_tag="ONT86_001316"
     CDS             21816..23540
                     /locus_tag="ONT86_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2352120.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            23537..24988
                     /locus_tag="ONT86_001317"
     CDS             23537..24988
                     /locus_tag="ONT86_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2352121.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(25461..27614)
                     /locus_tag="ONT86_001318"
     CDS             complement(25461..27614)
                     /locus_tag="ONT86_001318"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCR2352122.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(27616..28662)
                     /locus_tag="ONT86_001319"
     CDS             complement(27616..28662)
                     /locus_tag="ONT86_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2352123.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            28812..30101
                     /locus_tag="ONT86_001320"
     CDS             28812..30101
                     /locus_tag="ONT86_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCR2352124.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            30114..32816
                     /locus_tag="ONT86_001321"
     CDS             30114..32816
                     /locus_tag="ONT86_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR2352125.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            32801..33658
                     /locus_tag="ONT86_001322"
     CDS             32801..33658
                     /locus_tag="ONT86_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2352126.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(33729..34628)
                     /locus_tag="ONT86_001323"
     CDS             complement(33729..34628)
                     /locus_tag="ONT86_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR2352127.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            34797..36968
                     /locus_tag="ONT86_001324"
     CDS             34797..36968
                     /locus_tag="ONT86_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR2352128.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            37036..37377
                     /locus_tag="ONT86_001325"
     CDS             37036..37377
                     /locus_tag="ONT86_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR2352129.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(37653..39407)
                     /locus_tag="ONT86_001326"
     CDS             complement(37653..39407)
                     /locus_tag="ONT86_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2352130.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(39418..40254)
                     /locus_tag="ONT86_001327"
     CDS             complement(39418..40254)
                     /locus_tag="ONT86_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR2352131.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(40256..40939)
                     /locus_tag="ONT86_001328"
     CDS             complement(40256..40939)
                     /locus_tag="ONT86_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCR2352132.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        13302 a         7157 c         8493 g        12054 t
ORIGIN      
        1 agtgttattt tttggaaaaa ttcacaaata tgtaacgaat gtttttctgg aatttgctgt
       61 gttaaaaagc agtatgacta atataaatac tattgtcatt tatgcaattt tttatggata
      121 gtaattttag aggggaatca atgaaggaag aatagaaggg aaaaatagtt gcaaatacgc
      181 cagttagcaa aaatagcaat acatcttcaa gctttaaaat tcgagagaat catcttggta
      241 gtggaatcaa ctacaacaaa aggattaaac caattaatag atagaattac caatggaaaa
      301 atagaaaagg tagttattct atacaaaagt tgtttaattc gcttcggtta tgaacttagt
      361 gaaacccttt gcaatatatg gtacagaata tagaaattat tgacattaca gaaaatgatg
      421 aagatttaat acagattgtc actatttgta gatacctttt acaaggaaac cgagccaata
      481 aagctaagaa gatgattaag gagttgatgg aagacgatac aagttaagaa agtgaaacta
      541 cgcccaaata aagagcaaaa agagtaattg gacgtggaaa atgcaagaaa tgaaccatcg
      601 attcggtgga cagtttatta gtaatgacgg tttgagaaca tccatgacga gaatgaagga
      661 acatcctaaa tgcgtttagt tgaaccaagt gccaaataat atggccaaat aagcgatcag
      721 ttatcgatga atcccaagta ttctaaacta tatattcatt ttgatgctaa gcattgatat
      781 atttttttgg gattgatgaa ggttaaccaa tacagggatt cactgatgaa attctagagt
      841 ctgaccttgg tatcaacgat ttagtgattg tttctaatgg aaaagtataa aagtatataa
      901 gtacattaac aaaataacta ttagggttac gctccagctt gtgcaaggca ctggcaggag
      961 gaatcgtgaa atcataatta tggttaagcc acacggacaa ctaaaagtca tacaaagcta
     1021 tcgaatcaat caacagaaga caatcggcaa tcattagctt tgtttacagc ttacattgca
     1081 acgcttgagt tgaaccgaaa atacgaggga ggtaacgcag ccattttgag ttggggaatc
     1141 tttttagaaa attatcgggt accttgttgt ttagtcaaaa ttataagggg gagtaatagt
     1201 gaggaacaaa tttcataaac tgttaagtat gtttctagtt atgcttattg tgatcaatca
     1261 atttgggaac ataacggttc tagctgagtc tttagcagaa cagccagagc gttcgctgac
     1321 gatcgaagtt gatcaagagg aggctgcttt gggtgagcag ttccagttgc gagtcagagg
     1381 aacaccagac ctgctaaatg cattggtcat tgataagagt gaggatttga ttgaagggga
     1441 agtgaagcaa ctcaatgatt acgaacgtct gattacttat tcttcccagc attcaggtaa
     1501 atacgaattg accgcaagaa gcggtgatat tcaatcaaat acggtctcaa tagaaatcag
     1561 tgtaccagaa gaaagcacaa gtgagtcttt taatgtacca gaagaaagca caagtgaatc
     1621 ttttaatata ccagaagaaa gcttaagtga atcttttaat gaagccagat catctgagcc
     1681 atctataacg caggaatctc agattgaaag acaagaggag ctcttgaatt atgacatcgg
     1741 catctctgct gagcgcccca ctttgccgag caggcataca caaacgttaa agggatacat
     1801 tcaaggaggc gctggcgata ttcttgaaga atatggggag atggtcgtac gagttcctag
     1861 agcatcgatt ggcttaattg agttcattag agtggaggtc gctgatgatt cacctttaaa
     1921 gccactggca ttttaccaag aaggcacaga cgttgttttt ccatttacct ttgattatga
     1981 aggattaaat gaagccgctc agtcgtatac attcagtatc acgtttgaag ttgctttctt
     2041 tcaatcaggc ggtactgagc caaataatga ctttcaagtg agtcttactt atgctgagcg
     2101 agcagcaaac acggaatttt caattacgcc tattccttca tcaagaccca ttttttcgaa
     2161 attatactat ggctctagag aaatcgacag ccaaggaaat cttgggaatc tgctgattga
     2221 cgggcaagac gtgaccaaca gtaataaatt tattttgcag gtaaattatg aaggatcacg
     2281 aaaagacggt gtagtggtta ccgatgtact accgcaagga acaactttgg caccgaatcc
     2341 gactacatgg agttccactg gaaatagaga acctatttca ggtggggttc ggatcgcaga
     2401 agtaacgaat ccaggtgaag caaacgttag aactcattat gtttccgagg aatttgtgga
     2461 caaagtatct tttaataagg aaacgaatac acttgttgtg gactttggta atctagcaga
     2521 gggtcaatcg tattttattg aatatgctct tgaagtgacc gatgtcgact tgttcaatga
     2581 aggaagacaa gaggatgggt tttctcggaa tcgagcgagt atgacctaca ataatcaggt
     2641 aatgagccga gactaccgct tgagaatccc tgaaagtaat cttccggcaa tctctttaag
     2701 aaaaagtgtg gacaaaaatg ttatcaatct caatgaaaat gaactaacgt atactctgca
     2761 tgtctcgatg ttttctggaa caagaaatac gcttgttatt gaagatgttt tggaagaagg
     2821 catggaattt gtgtcatttc tatcagatat tccctctttc attgatgccg aagtcaataa
     2881 tggaatagtt cgtttcagtg cgaataatac gattggtggt ccgacaagtt ttaccctctc
     2941 ttttaaggtc aatgtagagg cgtatgcggt cggcgatcaa attttaaact ttgcaacgct
     3001 tgtgaatagt gacaatcgat tgaatacgag tactgtaaga acaaggaaaa tcgatgggcg
     3061 tattaaagta attaaaactg atgagaatgg ccaaaggctg gcaggagcaa aatttgaaat
     3121 tttatcaagc gattcacaag tgatacaaga aggtataacg gcagaaaacg gagaattttt
     3181 aagtgaaccg cttcctgtag gtagctatca agtccgagaa acagaagcac cggaagggta
     3241 tcttttagat gcgacggtac atgatgtttt tataaatgaa tactccatag aagaagtgac
     3301 gttaaccatt gaaaacatcc ttgatacggg tggtgttgaa ctaattaaac atgctgaagg
     3361 cagtggtgaa gtccttcaag gagctgtttt tgagttacag aaccgagaag gagaaacgct
     3421 acaaaccggt ttaactacag gagaagatgg aaaactggca attgacggtt tagcaccagg
     3481 agcatatcag ctggtagaaa ctcaagcgcc aatagggtat gaattagatg cgacaccaat
     3541 cgagtttgag atcgaaagaa gtcagacagc ggtggtagaa ttgacgaaag aaaaccgact
     3601 tacaccaggc ggtgtcgtgt tgacaaagat cgatgaccaa agcggggaaa tcctgcaagg
     3661 agctgttttt gagttacaga accgagaagg agaaacgcta caaaccggtc taactacagg
     3721 agaagatgga aaactggcaa ttgacggttt agcaccagga gcatatcagc tggtagaaac
     3781 tcaagcgcca acggggtatg aattagatgc gacaccgatc gagtttgaga tcgaaagaag
     3841 tcagacagcg gtggtagaat tgacgaaaga aaaccgactt acaccaggcg gtgtcgtgtt
     3901 gacaaagatc gatgaccaaa gcggggaagt cctgcaagga gctgtttttg agttacaaga
     3961 tgccaatgga accgttctgc agtcaggtct aaccacagga gcagatggaa aactggcgat
     4021 tgacggctta gcaccaggag tgtatcagct ggtagaaacc caagcgccaa cggggtatga
     4081 attggatgcg acaccaatcg agtttgagat cgaaagaagt cagacagcgg tggtagaatt
     4141 gacgaaagaa aatcgactta caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag
     4201 cggagaagtc ctgcaaggag ctgtttttga gttacaagat gccaatggaa ccgttctgca
     4261 gtcaggtcta accacaggag cagatggaaa actggcgata gatggcttag caccaggagc
     4321 atatcagctg gtagaaactc aagcgccaac ggggtatgaa ttggatgcga caccagtaac
     4381 tttcaagatc gaaaaagaac aagaagtggc gatcttttta acaaaagaga atagaaaagt
     4441 tgctgatagc tcagatataa gaggaaacaa tccaacaagc agtggaaata agcatcttcc
     4501 aaaaactgga gaaaccatca ttagccagtt tatattgtca atattgggtg tgctacttgt
     4561 tttcatcagt ataaaattta gaaagaaacg aaatatatga gctaaaataa aaaattgaat
     4621 aagcatagtt tttaaaatca actagtaccc atcaattgaa tggaaactac cgattttaga
     4681 aggggtatac aaacaaatat tagtatcgga gtagagaagg ttgaacccaa ccagtaaata
     4741 agtaaagaca attttgtgct gcgttgagaa gtgaaaaata aacctttgta gtaatactag
     4801 ttaataaaaa atatacaaaa acactgcgaa aatcattctt gagtttcgca gtgttttttg
     4861 cctaatgact caagtttatt gggtaatact ttgattttct ttctacaaat aatcagcgat
     4921 cagttgtaat tttttctcta gttttaatgg ctctttgtca ataaggagaa aaacaagtcg
     4981 ggtcttttgt ctcggaaagc ttcaaccaac tcgtgaataa aggttacgcc tccttgaaag
     5041 gcgtggaaaa accaagtaat cgatcaatca ccaatttcgc atttggtaga acatgtttgg
     5101 tgagctgaaa gtaggtggcg ttcatgtctg taactaagaa ttctatttct ttaggattag
     5161 tgcagctaag gaaatagttt gttaatcgag gaattttttg tgtaggtaaa ttttctcgat
     5221 taatttttct gtctcgccat ccgcggaaat aaaactcagt ttatcctcaa cggaaacgtg
     5281 agaacgaaat tcacaaccct caacactcaa gacagaattc ttttggaaga ctttggtaaa
     5341 tagcttctca actctttcaa tgtgcgaata acggttgtca aagataccta acaacttttc
     5401 gaaataaaaa ataacgacac tttttcagtc agtaaagaag tgattttatg tctgacatga
     5461 tttgaaatcg agtgccttgg acggataaaa taactttgag cgttccaatg gcttcgacag
     5521 tttttacagg tatagcgctg cttttttagg cgtataacca ttggcatatg attatattat
     5581 tcaaagtgga caatggtttc cttcttcttg tttttcacca ccattaattt cctatttcca
     5641 tctaccacag cagaaccaca atttctacag acacatggag cagggaagag aactgcagca
     5701 acgatcaatg tttttttctt ctgtaaggtt tcgtaagaga cctctgtaat catcaaattt
     5761 ttgtctattg atctcagaat ttttttgata gaaccattca taaagcatat cgttctctca
     5821 gttgtttatt ttgtggtgat ttaatcatac aagaaaacga tatgtttttt tataaaaaca
     5881 tgaataggaa aaatagttga caagaaaatg acaacgccat atgatgttcc atagtattta
     5941 caacctgtca tagatgactg taaaaaataa aagtaaaaag aaaggacgac aacaggtgga
     6001 tttttatcag tctctacaac tagatccgtt tattttaaaa caaaaaatac gagaagcagc
     6061 ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt ttacgatcat tattcatcgt
     6121 attgtttgcc atttgtttta tcattataat tacaacatta tttgaatcga agcataaacc
     6181 atacgctgtg gttttattct gtatgttgat gagtattcgt tttgttgact ttggttataa
     6241 gatttcccat tcgatcatag gtttagcaat cgtcatgttc tcactattag tagcaccgta
     6301 tgtccagctg atcaaatggt cagttatggg catgttgatt cattttattt tactaagcag
     6361 catattaatg gcaaccgcta gtgatcccaa aatggggaat gcttctttgt acggcttttc
     6421 atatctcttt attgtttatt cgttacctaa agattcgttg aacaagaatt tctttacaca
     6481 aacaagtagc ttattatttt tgtttttttg ttggttttca gttctccttt acagaaaaca
     6541 tcgagaaaag aacaaagaca aaagcttgtt taaagagatt tttttgaaag gcatgtattc
     6601 gcaggaaaaa atttggatgc tcatctatgc atttggcatt agtcttttga tcgttgcagg
     6661 agagtatgtc ccttttcagc gattgatgtg ggcagggttt gcattttctt ctattgtatc
     6721 tagctatgga ttgatgtcga ttggtttcaa agaacgagca gtagaccgaa tcattggctc
     6781 attgattgga tgtgttttat ttattggtat cagccaattt attcctttta actgggtcgg
     6841 tatattaggt ggtctagctt taggtgtttg ttctacctat agatataaaa cagtttttaa
     6901 ttcttttggt gctttggcaa tcactgccag tttattcggg gttccaggag cagtaacgat
     6961 ccgcattttt gagaatatcc ttggggtatg tttaggaatc atgtatattg gggtgactga
     7021 aatacttatc cgaaaaataa gagaaaaaca cggtttgaat cattagatga gatcgaataa
     7081 aaaataagga aaaagtgaag atacacatgg atgataaatt gctatcattc catgtgtatc
     7141 tttttacggt aaatcagtga aacgttcgac tttcattttt aatttatact ttttacggag
     7201 ttcgtttatt tgagccatca tttcggattt gtgatgcttt tcgattgctt cttcatcttt
     7261 ccaacggtcg atcagtaaaa gagattctgg atcttctagt ggaaagtaat agtcatattt
     7321 ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt ctttcgacca tttcttctgc
     7381 aaattttctg gcttgtccat ttgtacttgt atacaaaata ttcactgtga tactcactaa
     7441 atcccctcct gaaaaaattc tatcactagt atatcacggt gccagaaatt attattcaag
     7501 atatgataca cattttttcc gcaatgctac ggaaatatat attttgaata gaaaacgctt
     7561 acaagatatg cttggtttat aaaaataaaa ggagtgattg atttatgaaa aaagcattga
     7621 ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc aaagacaacg gatttcttta
     7681 agcaaaaaag agaagaggta ctcgtagatg cagtttcagc tacagaaggc gataatatga
     7741 ttaagaatag tgattttgat ttatttctga tcagcccgca aacgacgatg tttttggata
     7801 aattcaaaaa attaggagaa tctgttggaa agcctgttgt tagtatccct ttccaagcgt
     7861 atgtcccaat ccctacaggc atccagaagt tagcagaggt gatcgaagag aacatatgat
     7921 ggaataggag tggatgataa tgaaacagaa ggaaaaattg ctgttgagat atttgattga
     7981 tcacaagaag gagtatgtca ccagtcaacg tttagcttct gaattgttat tatctgatcg
     8041 gacaatccgt aattacttgc agcggatcaa agagttagta gaaaaaaacg gaggtaagat
     8101 cattgctaaa ccaggttatg gctaccaact gcatatttta caaagactga catttgactt
     8161 gtttttgagt cggcaagaaa tagtacctgg atatgctaga gaaccgcaag gattttatga
     8221 gtccgaagat cgacaaaagt atatattgaa ccaattgctt ttggaagatg ccattctttt
     8281 tatggatgat ttggcagaag agctttatat cagtcgttcc agtttgacaa aggacatgca
     8341 agaaatcaag gagcgattga ttccatattc actaaaaatc gtttctaagc atggtcaagg
     8401 gacatggatc gaaggggaag aaagaaatcg acggcatttt atcatggata ctttttttgg
     8461 aaaccattat ggaaattcat tgaaagaata tttagggcaa agccgttttt tcccagacat
     8521 cagttttgaa gagctagtca ttatcattct cgatgaaaca agagaagcta agttaaaagt
     8581 ttcggatttt atcatacaga atctgacttt acacttggct ttggggatca aacggctccg
     8641 agaagggttt gaaatcaagg aattaggcat cgaacaagaa gtttctaagc gagtagagta
     8701 tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc gcaggtgtat cgtttcccga
     8761 agaagaaatt gcttatttga cattacatct catggctaag tcgaatcaca aagaaaatca
     8821 aaatgatcag gaactatcaa aggaattagc gaaagttctg aaaagattgt ctcaggaatt
     8881 tcatttttca ttagtagagg atctccagct tttcaatgga ctactcgatc atttgtcacc
     8941 gatgatgatt cgattgagcc gaggtattcg cattgagaac ccattgactg aggagataaa
     9001 gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat ttcagcaaca tgccggcttt
     9061 gaaaaactat accatcaatg aagatgaatg ggcctatttg gctttgcatc tcatggcggc
     9121 attggaaaaa gaaagagctg cgcataaact acatgcattg attatctgtg caacgggtta
     9181 cggcagtgcc caactattaa agaatcgcgt agtcagtgaa ttcggtaaaa atattacagt
     9241 ggtaagtgta aaaggctatt atgagatcaa tgaaacaact ttaaaaggaa tcgacctgat
     9301 tatttcttct attgatcttt cgacaatgtt tttcaaaatt ccagttttac atgtcagtat
     9361 ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta gttggagaat ctatacccta
     9421 tcattcctca gatagaccga taccagcatt ttctctacag caaaaaaagc agatttacac
     9481 cgaacagttg tctgaaaaat ttttcaagac ttatacgtac gaaccgaaaa aggaagaaat
     9541 tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa gaaggatata taagagaaat
     9601 gcgaaagcag atccagcaac gtgaaaatat gggacaaatc atattcagtg atacagtggc
     9661 agtcccacat cccgctgttc cagttggaat cgaaacaaag gtagccgttg cattgattcc
     9721 ttctgggatg cagtgggatc aatatcaaga aattcatttt gtttttttag tttcgccttc
     9781 ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca atcgttaggt tagttgacca
     9841 gctaaaagta cagcaggaaa tcttagctga acctattttt gagaatttca gcagtaaatt
     9901 tatcaaactg atcgaataag gaggaaaaca gatggctgaa aaaataaaca gtgaaacact
     9961 acaagtgact tcttttgata tcattttcca tagcggaaat gcacggacac tgatacatga
    10021 agcatttcgc tcgatgcgta aagagatgtt tgaagaagta gaagagaagt tgcgacaagc
    10081 aaatgaagag attcttcatg cgcatcagtc acaaactcaa cttctgaaag aatatgccag
    10141 cgggacaaaa atagaaatgg aaattatcat ggtccatgca caagatcatc tgatgacgac
    10201 gatgacatta ttggaagttg cacaggaaat gaattatttg tatcaaaaaa ataaatagaa
    10261 aggaaggatc attgtgcagg atacagtaaa aaggaaaatc gaattagaaa aaaagcaact
    10321 tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta cgttacctga ctgccttttt
    10381 ctttttcatg aacatgcaat ggatgattct gcttctatca gctaaaagtt taggaagtag
    10441 tttaccgatg attctgattt tagctatttt gcctgcagta ggagaacaag taaagcttta
    10501 tcgaaaacac caaacgaacg ttccatggac aaagtggtat tttttgttcc agagtgtctt
    10561 taatttatta gtgattccag tgttgtttac ctcaggattt acattcttgt atccttttat
    10621 ggcaaataac aatggaggac agttatttgt tttcattctt gttgtaagcg gtatttttgc
    10681 cagtgcattt atacagtatc gtttaaagaa aatttcactc aaccaagacc aacagtatat
    10741 ccgtattaaa caatatgaaa aagcgttata tttaggaaag gagaataact aatgtttgat
    10801 tttttgcaga aatacctgat gggacctatg gggaaaattg cgcaacttaa aatcgttcgg
    10861 gctgtcatgg cagcggggat ggcttctatt cctttcacga ttgtaggctc gatgtttttg
    10921 gttttaaata ttttgccatt accgttccca tttttagaag gattttttaa tgcaactttt
    10981 ttcaaagtta gtgacttgta catgatcgtc aatacaatga cgatggggat tttatctatt
    11041 tactttgcca ttgtgtttgc ttatgaatta acgtcaattg aacgagatga acaaaaatta
    11101 aatgttaatc cgctcacggg ggcattacta tctgtttttg cattctttat gtgtatccct
    11161 gaattgatca tgactgatgg taaaatcagc ttgattgctt ctatcaatga gacagaaact
    11221 gtagtaaatg gtattaggat gggctctttt gtcgaacgtt taggaacttc aggaatcttt
    11281 acagctatta ttatgtcgat tattgcggtc gaattgtatt gtatgtgtgt caagagaaat
    11341 tgggtcataa aaatgccaga tgttgttccg ccaggagttt ctcgttcttt cactgctctg
    11401 atcccaacat ttgtcattgc gtttgtggtg atgatcatca atggattatt agttgcacta
    11461 ggaacggata tcttcaaaat gattgctatc ccatttgggt ttgttacgca gttgacgaat
    11521 acatggcttg gtgtcatggt gatttatttc ttgatccatg cattgtggat cgtagggatc
    11581 catggagcga atatcatcac ttctttcttg accccgatcg tattagctaa tatggcagct
    11641 aacgcacaag gcgcaaatta tccgttggca ggagaattca acaattctta tgtaaccgtt
    11701 ggtggatcag gtgcgacttt aggattgatc atctttattg cattcctcgc gaaatctgat
    11761 cagctgaaag tgttaggaaa agcctcactt gtaccaggat tattcaatat caatgaacca
    11821 atcattttcg ggatgccaat cgtctacaat ccctatttag cggttccgtt tttcttagct
    11881 ccgatggctt ctgctacact cgcttatttt gccattcggc tggaaatcgt caaaccgatg
    11941 cttgcacaga tgccgtggcc ttcaccagtc gggatcggag catttgttgg tagtggtggt
    12001 gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca tcgcattcat tatctggttc
    12061 ccatttatca aattctatga taataaatta ttaaatgaag aaagagaaaa ggcagcagaa
    12121 ttagctagtg aaaagttaaa atgatttcta agaaaaaagg aactgtgaca aaaaatcaca
    12181 gtcccttttt ttcttagaaa tgggaaacca ctagtatata aataccatta ttcttcaatc
    12241 aaccgcttca cgattgcata atccccttca taaggttcat ctacaccatt gattttttga
    12301 tagggatcca cgccttttcc cgcaataacg actgaatctt gcggagcgct ttctgctaaa
    12361 gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa cgatcaaatc tggattagta
    12421 attgcttcat tgatttcttc cgcgattttt ttcggatctt caaatgctgg gtcatctgcc
    12481 gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc caaaatcatg tcgacgggag
    12541 attgctttgt ttcctgggct gcctaataca acaattacac ggccatctgg atgttcattt
    12601 ttggcaaatt tcagcaatgt tttcaagctt agataattat gtgcatagtc cacatagaca
    12661 tgcgctccat tggattggat caattgatcc attcggcctg gtacacgagc ttcttttaat
    12721 ccgtcttgtg catcttgact ggaggcacct gttagtgctg cggcaatgat cgcactggct
    12781 gcattttctt ggttgaatcc tccagccagc aaaatatcat aagcagtatc gcagatagct
    12841 aagcgatctt catgagaaga taaagtaaaa ccgtgagtac ctttatctga acggatcact
    12901 tgataattgc tttcttccga acgaccataa gtgatcgttg gaatctggaa tagatcggct
    12961 gtttccctaa gtaaatcaaa atagtcactt tcatgattta aaacaactgt ttttgaattc
    13021 aagatcaatt gtcgcttgca atagaaatag tcatcaaatg tcggatgttc gatcggactg
    13081 atatggtcag gcgagatatt caagaagatt ccgacatcta aagtcaagcc gtatacgcgt
    13141 tgtgttttgt aagcttgtga agaaacttcc atcactagat gagtcatacc attttcaact
    13201 gcttctgaca tcatacgata taaatctaat gactcaggcg ttgtcaaatg ggatttgaaa
    13261 tatgttttgc catctaacgt tgtattcata gtagaaagca atgctgtttt tttatttgtt
    13321 gtgtgatcca agattgcttt tgtaaagtac gcagcagttg ttttaccttt tgttccagtg
    13381 aatccgatca ctttcaattt attttgcgga tagtcgtaaa aagccatagc aagaagcgcc
    13441 attgctttac ggatatccgt tacgatgatt cctactgctg aattttcgta tggttgttct
    13501 gatacataat attgaatacc ctgttcgatt gctgaatcca aatattcttc tttgaaattc
    13561 attcctttac agaaaaacaa agtagaagca tcagcttttc ttgaatcata acttaactga
    13621 agaaattcag ctttttctgg tacatcaaga tgccagcctc ttgttgaaac aaattctttt
    13681 aatagatttt cttttaatag taaatgacgg atgtcttcta aagagaagct cattgattga
    13741 tccctcgctt tattcaattt cttggtttat tatagcacac tctttttaat atatcgtttg
    13801 tttccgctat gacaaaatcc agctttattc aaattttctc gacacagggg acaagcggat
    13861 gagtattttc ataaacaatg aactgactgt aatagatagg agaacgaata gtgtctccaa
    13921 caatccaaaa acataataat tcaaaaagac gataagacta ttggtaagta aaagtccttg
    13981 cggcattgga agccgaaaaa atttgtggaa aataatcggc ggtatcgtta taccgccatt
    14041 tgaggccccg actgtgtaca atgtgtaaac cccaagactg aacaaaagac ttcctgaaat
    14101 cagtgaaaag aaatgtgagg agatcatttc cacttttgga acaatctcta aaacaacagg
    14161 aaaaagcaga cttcccgcta cagtcttaaa aaatactgat tttcctaata ggacagcggc
    14221 tagaagcagc atcataagat tgatgctcca aacagtaact gagcgttgga tagaaaaggc
    14281 cgtttcaact aaatgtccga tggcgcccac tccggcggaa gctatatcgt gagggagcaa
    14341 gaaccaattc aatccaactg tgataaagaa aagacctaaa aatataacgg gtagtttttt
    14401 tatttcttgt gtcattgata tctcctataa atgttagcca taaaaggctg tatatttctt
    14461 tactttacca aaaaaacgaa accgaattca aaaaaacaat tgttcttata aaagttatat
    14521 aaaatcgatt gccagtttct gttttaccaa ttaaaatgat agtaatgttt tgtagaaagt
    14581 tggaatctga ccgatggata atcaaagaaa accagcatta gatcaattaa caccgcaaga
    14641 aaaaatggca cgaggctctg cttggatgac cgccagtaat atcatttcaa gattgctagg
    14701 cgctgtctat atcattccct ggtatgcttg gatgggcgaa aatgctaagg ctgccaatgg
    14761 attattcaat atgggataca atatatatgc attgtttcta ctgatctcta cagcaggaat
    14821 acctgctgcg attgcaaaac aaactgcacg ctataattca ttgaatgaat atggcacttc
    14881 acgacgatta tttatccgtg cattacagat gatgggcggg ttaggcctgt tgtttgcttt
    14941 gttcatgtac atcgcctctc cttggctagc tcatgcttct ggtggcggag aagaactgat
    15001 tccaacgatg cgttctttga gtatcgctgt attggttttt ccttgtatga gtgtgatacg
    15061 agggtatttg caaggcaatc aagaaatgat gccttatgcg ctgtcgcaga ttgtagaaca
    15121 ggtggcacgt attttttata tgctattggc tacattcatc atcatgaaag tcatggatgg
    15181 tgactatgtg acggcggtca cgcaatcaac tttcgcggca tttatcggta tgctagcaag
    15241 tattcttgtc cttttgtatt ttttgaagaa acatcaagcg tatacatcgg cgtttattca
    15301 ctacagcgaa aacaaaataa atattgcaac gaaggaattg ttgttagata ccgttaaaga
    15361 agcgattccg tttattattg tgggttcagg ggtaacgatc ttcaaattag tcgatcagtt
    15421 cacttttatg aagatcatgt ctgcatccac cgagtattcc aatgcacagt tactggatct
    15481 tttttcgatt tttagtgcca atccagataa gttgacgatg gtagttattg cattggcaac
    15541 atcgatcgcg tctactgggt taccgttgat tacagaagct gttacgttga aagatcgagt
    15601 aggtctggca aaattgacaa gcggaaattt acagttgttt tcatttttta tgtttccggc
    15661 gacatttggt gttatgttac ttgcctatcc gctaaataca ttattttata caccggattc
    15721 tctaggaagt aatgtactga tccaagcaag ttttgtagga ctcttcctag gactatatat
    15781 gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag gcggcaatta attatttggt
    15841 tattgggttt ctagtgaaac tccttcttca atacccagcc attcgtttat ttgaagtata
    15901 cggcccttta ttggcgacaa tgatcggatt tgctgtgtct tgtaccttga tcttaaagag
    15961 aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg cgcaggacat tgctgatctt
    16021 tattcttacc ttgattatgc ttttggcttc gtggatcaca aagatgattt ttggtgcttt
    16081 tttaaacgaa gatagcaaat tccagtctct attgttgatt cttgtagtag caggtatagg
    16141 ggctttagtg tatggttatt tagctttgaa aatccgcttg gcagaaagac tactaggacc
    16201 aggaatagcg cgtttacgac ataaattaaa aataaaataa gtgaatcaga agagctagat
    16261 tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat aagtttttag cagatatggg
    16321 acttggcagc cgcaaagaag tcaaaggatt aatcaaaaaa ggaacgatcg aagtcaatgg
    16381 acaagtggtg aaaagcgata aagcgcaagt aaatgaattc gaagatgcgg ttacttattt
    16441 aggagaaatc attacgtatc aaaaagattt ttactacatt ttgcataaac ctgcaggagt
    16501 catttctgct actcaggata atcatgatca aaccgtaatc gatttattgg aagaccagga
    16561 ttatcgggaa gatctttttc cagtcggccg attagataaa gacacagagg gcctcttgat
    16621 tttaacgaat gacggggtgt ttgcccatca gttactatca cctaaaaaac atgtggagaa
    16681 agaatatcta gctgaggtta gcggtataat gacagaagaa gatatccgtt tatttgcaga
    16741 aggactgatt attgatggcg atgagcagac attgccggca aaactccaaa ttgatcaagt
    16801 agatcaagag acagaaactt caattatccg tctgattttg catgaaggaa agtttcacca
    16861 agttaagcga atggtcaaag cagtaggaaa agaagtctta tatttaaaaa gaatccgtat
    16921 gggaaaattt tggctgccta aagatttgaa taaagggcaa tatcgaccca tgaccaaaga
    16981 agagctagaa caagtgagag aaaaataatt gaatggatag ctaaaagaat aagcagagta
    17041 gttgtagcgc catctgaaaa gcagacttgt ccgtctgact ttcggatggc ttttttgtga
    17101 aatttcggct tatttccgta gaagctactt ctggaacacc gtttgttcgg aataagggac
    17161 tgtgacaaga cttttgttac agtccccatt gttccttgtg actcagagtt agatagtaag
    17221 gacacgatct ccctaatact caggccaaaa cgctgaattc acaacctctg ataaacggtg
    17281 ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttc
    17341 ccgttttttt ctctaatact caggccaaaa cgctgatttc acaacctctc ctatactttg
    17401 tttcattttc cctatacttt gtttatttat tctcattatt gaaagcgcta taattacgtt
    17461 gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat cattttttta
    17521 tgaatctatg gcgttataaa gcacttgtcc tgatggctgt accgggtatg ctttggatga
    17581 tttttttctt ttacatacct gtaatggcga atgtcgtagc attcaaaaat tttcatattt
    17641 cagcagatgg gttcttggct agcttgaggg aaagtccttg ggtgggttgg gaaaatttca
    17701 aatttttatt ttcttcggat caagcatttc tcatcactaa aaatacaatt ttatataatg
    17761 tagcatttat ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt
    17821 tgagaaataa acgactcgtt aaagtttatc aaacgatgtc cttactacct tattttctat
    17881 cttgggtcat tatcagttac tttgtttacg cttttttaag tcctgacaaa ggaatattca
    17941 atcagtggat cactggacat ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt
    18001 ggccgtttat cttggtgttc attggtacat ggaaagggat tggttataac agcattattt
    18061 actttgcatc tgttatggga atcgatccga cttattatga agctgctatg gttgatggag
    18121 ctagcaaatg gcagcagatc aaacatgtga cgataccaca acttattcca ctaatgacga
    18181 ttttaacgat tttagccgta ggaaacattt tccgagctga ttttggcctg ttttataaca
    18241 ttcctaggaa ttccggtgca ttatatgatg tgactcaagt tttggatacg tatatctaca
    18301 atggcctgac atccacagga gattttggta tgacggctgc tgcaggactt taccaatcag
    18361 ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt gatagcgatt
    18421 ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg
    18481 ctctttcaat aagacggcta attttttctc taatataatg attgcactct ttgctatttc
    18541 ctgtattttg ccatttatat ttgttatcgc catctctttt accgatgagt ccatattgtc
    18601 gactcacggg tacagttttt ggccagaaaa gttcagtata tttggttata cgtacttgtt
    18661 cgaccagatg caggataaaa ttttccaagc gcttttcgtc actgtacttg ttactgtttt
    18721 aggtacactg atcaatagca cggctacttc tttatatgct tatgcgattt cacggtcaaa
    18781 ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg
    18841 gatggtggcc aattatcttg ttatgacaaa tcttcttcag ttaaaagata cgatttgggc
    18901 attgatcttg ccaatggctg tcagtccctt caatattatt gtgatgcgca cattctttaa
    18961 acgttcggtc tctgattcga ttattgaatc ggcacggatc gatggagcta gcgaactgcg
    19021 aatatttgtt cagatcgttt taccgttagc tgttccaggt attgcgacaa tcagtttatt
    19081 tgccgcattg ggttactgga atgactggtt caatgcttta ctatacatcc aagatgataa
    19141 gctagttcca ctacagtatc ttttgatgaa aatccaaagt aatattcaat atctgacaca
    19201 aaattcagga gcaggaagcc aattgacagg cggattagcc tcagttccag gagaatctgc
    19261 cagaatggcg atcgtagtca tctcgacgtt gcctattgcg atcagctatc catttttcca
    19321 gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa acaaaaatat
    19381 aaatcataaa attaggagga agaaaaatga agttatggaa gaaactagcc tttacaggtg
    19441 tttcagcgtt gttgttggga acattagcag catgcggagg ttctggttct aaagaccagg
    19501 cagaagctag caattcggac actttgcaga tgtatcaaat cggagataaa ccagataatt
    19561 tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca
    19621 atatcaatta cattggctgg ggcgattacg aaaagaaaat gaacgtcatc atttcttccg
    19681 gggaaaatta tgatatcgcc tttgcgaaca actatgtttc caatgcccaa aaaggagcct
    19741 ttgctgattt gactgaatta gcgcctaagt acgctgaaaa agcttataat gatttagatg
    19801 aagcatatat taaaggaaat ctggtcaatg gaaaattata tgctttccca gtcaatggaa
    19861 atgtctttgc tcaacaagta cttactttta acaaagcttt actagataaa tacgatcttt
    19921 caattgatgg aatccaatct tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa
    19981 aagaaccaaa cacggcagct tttgccattg gacaaggctt caaagtccaa gggaactttg
    20041 attatccttt agggaatacg ttaccatttg ctattgatct ggatggagat gattcgaaaa
    20101 tcatcaacca atacgataat gaaacgtata tcggactgtt acgtacaatg catagctggt
    20161 acaaacaagg actcattcca agtgacgctg caacgagcaa taaggattat ccactagaag
    20221 gaaatacttg gctaatgcgt ggagaaacac aaggaccgta tgattatggc gatacaattt
    20281 tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat tactgttcca ctaaaatcta
    20341 cagctcaagc acaaatggca aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat
    20401 cagtagaact tctagggctg cttaacagtg atccagaatt attgaatggg cttgtttggg
    20461 ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga tgataaaatc aaattgctag
    20521 atggttatca gccaaataca catatgtctg cttggaatac aggaaacaac aagattctat
    20581 atacacaaga gtctattacg gatgacatga ttgccaaacg tgatcaatcg attgctgatg
    20641 cagaaacttc accgatttta ggattcagct tcaacactga cagtgtcaaa acagaattaa
    20701 gtaatatttc taatgtcatg aatcaatatt tagatggatt gaatacaggg actgttgatc
    20761 cagatgaaac attaccgaaa ttgaaagacg ctttggacaa ggctggctat gataaggtat
    20821 tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga ataataatga ataaagacaa
    20881 aggggttgtg acaaaagacg tgtcacaacc tcttgttccg aataaacggt gttcaaaagc
    20941 cagtttctcg gtattaagct aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt
    21001 tttcttacta ctcgaaacta agcggctatt tcacaaactc ttggtaaacg gtgttcaatc
    21061 agctaaccct cggtattcgc tcacaaaacg acaaaatatg aggaactatt tttccgtttg
    21121 tttctctaat aatcaggtca aggtgctgat tgcccaacct ttttttgtat aatgagaaag
    21181 tataaactta atgtttggag gagaagatat gttagggaag gttttggaag agttatttat
    21241 ccggatctgg gtcattataa aactaacctt gtatttctgg atatatacat ttgccggcgg
    21301 gatcatcttt ggattaggtg cggcatggaa aacagtcaat gaactatttt atttgtacgg
    21361 attcgagtat aaagaaatca caataaaaag aggctggaat atatataaaa gaaatttttt
    21421 aagaggcaat ctgctattta gtttattttt atctggtact gctttattga gttacaattt
    21481 atatttatct gttcaaatca aagggctcat ttttttagct attgatttta ttttactttt
    21541 tgccttgctc tgcctatttg ctacttatca atacagtatg attttagata gtgaatatac
    21601 gatctctgtt cctaatctgc taaagctaag ttttatttcg gttttttcca gcttttcgtc
    21661 ttttctaaaa ataattattg gtagcggtat aattttaggc gtgacttggc aattcaaagg
    21721 attgatcctg tttggtgtca ttggtctttt gacggtttgg aatggtacga tgacaacaca
    21781 ttggcgtgaa gaattagata aacagttgga aagttatgaa taagtttcgg attctatttt
    21841 caaaaggaac cttactaaat cgtctgttgc ggaaatacgc ggttatcatg atcggtgtaa
    21901 cgatgactgc taccgttatt tttagtatcc atacttggga acaaaatcaa aaacaggcag
    21961 aaaacatgac aagtgatgcc gttcaaagca caagcagaat gctgaatgat aaaacaacct
    22021 tgagtaggat catcaaaaac cagcttgtag ggaattccga aaagatcgaa aatgtgacga
    22081 cctacttaac aaaacccata gatcagtatc tgatgtatgt atacgaacag caaaacagta
    22141 ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat tatgaagaac
    22201 taagtgcgat atatatcgtg ttgaatcaac taccggagta ttatgaatct tctagagaaa
    22261 acaaaggtgg ggaaataaaa aaggggacac caaaattact agacgccttt tatatcaagt
    22321 tgcctatcac acaaggagga gctgagtcag gaagtatttt tattggtttt caaaagaaag
    22381 aactagatat gattttagaa aatctaacgt cattcaatgg actctcttta tacatgattt
    22441 ctggaacgac aaatcgacta tacacattcc atgataataa aatagctaaa gaaacgtttg
    22501 aaagacaaga aatgatcatc acagagagtc tgaagaaaaa ttcacaattg ccgattgacg
    22561 atctggaaaa aaacaatttt gttcaacacc aagaactgtc tgatgattat cgtatattag
    22621 ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc ccgctgatca
    22681 ttggtatcat tattttagac aatatactgc ttatctttct ttataaaaca ttcaaacgat
    22741 attcacagca agtatctatc gtcatgcagg ccatggatca aacagcagca gggaatttag
    22801 agacgcggat cgacataaca aacacggaat atgaactgag ggaactttca atcggtatca
    22861 acgaaatgtt ggatagcatc aatcagtttg tagaagacat ctacaaatta gaaatcaaac
    22921 agcaagacgc acatatgcgg gcgcttcaag cacagatcaa tccacacttt ttatataata
    22981 cattagaata tatccgaatg tatgcaatca gcgaaggaag tgaagaatta gcagatgttg
    23041 tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca agagaaaacg atcactttaa
    23101 aagaagaact ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt tatcctaatc
    23161 gagtagccta tcattttatg atcgatcctg atttggaaaa gatcgaagta cctaaatttg
    23221 tgatccagcc attagttgaa aattatttta aacacggtat tgattttact cgattcgata
    23281 atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc atcaaagata
    23341 atggaaaagg gatgactgaa aaacggctaa aacagattga agaaaaactg tctcatccta
    23401 aagtggaact tcacggttct atcggtctgc aaaacgtgaa tgaacgttta cgtgccagct
    23461 tcggcagcag ttattatatg tcgcttgaga ataatgagac aggtggactg accgtttcta
    23521 ttacattcaa ggagggatga gcgtgtatca agtgttattg gtagatgatg aatacatgat
    23581 tttgaatggg ttaaaaaaga taattgattg gcaaagttta ggctttcaaa tagtagcaac
    23641 agcagagaat gccaaagaag gactagctgt acttgaacag cgacagattg atttagtagt
    23701 aacggatgtg acaatgccag aaataaatgg actggaattt attgaagcgg ctcaaaaaga
    23761 acgtcataat tttgaattta tgattttatc aggataccaa gaattcgatt atttgaaagg
    23821 aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc gtgaataagt tcgaattggt
    23881 tgaaagcctc aagaaaatca aaacacggtt agatcagcaa aatgaacaaa aaaatcaaca
    23941 agaaatctat caagaactgt tattcagtca atggttgaat gaagaattgg atgaaataag
    24001 tgaagaagaa ataataggac aaataagtga gaagcagcat aggatagtat tgattcaact
    24061 ggcaagaact catgatactc ttatcaatag atggttgcaa gaacggcaac aaatctttta
    24121 ctatcaacga aattacggag accgtgtatt gatcacgttg ttgttggagg cagatgaagt
    24181 tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat caggaatggc tgatcagtat
    24241 cggggaagag acagattctt tggacaaaat ccctgaaaca ttccaacaag cgaaagacaa
    24301 tctacagttg catcaatttt atggagataa agacgagcat gttttttacg cagtacaagc
    24361 agaaacaaaa gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc aaaataaaca
    24421 attggaagtc gctcaaaaaa tgatcactga tttttttgaa cagtttcagt tagcagccat
    24481 gatgccggaa gatatacgat attccagttt tttactgttt atggagattc aacgggaatt
    24541 gattgatctg gaagatgagg aatatttaca aggaatcgaa aaaatccatc aggcaaaaac
    24601 ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa aagcatcaga gacagaaaaa
    24661 gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac cagcattatc aagaaccact
    24721 tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc atgtatctag gacagctgtt
    24781 caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac catttacgta tggaaaaagc
    24841 gaaacaactg cttttgcaca gtaatcaaaa catcaatgag atagcaagtg agattggcta
    24901 caacaacacc acgtatttct ctaaattatt caagaaaatc gtcggcagat cccctaaaga
    24961 gtaccgagaa aacagtggag aatcataaaa gaaaaagcca atgtaatgaa ataacttata
    25021 aaaaattgtc tagaaatcta attttgggct gttcttatta cttgaggcaa ggcagctttt
    25081 tcatgacctc tcgataaacg gagttcaaaa gccagtttct cggtattaag ccaaatttat
    25141 gcaaaaatgt ggaaagccat tttcacaaaa tttttcttaa tactcgaaac taaacagctt
    25201 tttcacgacc tctccactaa acggtgttca atcagctgac cctcggtatt agctcaaaaa
    25261 atggcaaaat atgaggaact attttgccat ttgtctctct aatactcagg tcagaacgct
    25321 gatttcacaa cctctcaata aacggtgttc aaaagcttgc atctgcggta ataagaccaa
    25381 ccaaacgaaa aatatgagga tctatttttg cttggctgtt cttattactt gatgcaggac
    25441 agctttttca caacctcttg ttaattatag tttgataagg aaaaaggcaa atcttttgct
    25501 tgatatttct tagggtgagg gatgatccct aattggtaag agatcgtgcc gcctttcagc
    25561 aaatcttgat gggtaaaata taatttcgta tattctctgt ccatccgttt gattttatgg
    25621 ataaattgtt gctgcggccg attaggcgtt gcttcaatcg tcaattcctt tccatttgac
    25681 aaaagcaaaa cagccttgtc aattaatggc ataccgataa catattcacc ggttccaggc
    25741 gtgacaggat aaaatcccag actattgaag atataccaag cggccatact tccattgtcc
    25801 tcatctccag ggtaacccat tggagaatcg tcgaaggtct gagtcatcaa ctgtttaagc
    25861 agtggctgtg ccatttcagg ttgtcctata taactgaaca agtaaggata gtgaaaactt
    25921 ggctggtttg aaatagctaa ttgtccaaat tctatcgcgg ccatctcact catttcgtgg
    25981 atctcaaaac catatcctcc tgggtcaaac gttgggggct gattgcacag ctcaatgagt
    26041 ttttctttat atctctcact gccaccatat tttccgatca gtcctgaaaa atcgtgataa
    26101 acagcaaagc tgctttgcca agcacttcct tctgcataat cttgtcccca acgattagct
    26161 gaaaatggtt ctctgaactt gccatcttta tcttttgaac gcataaatcc tgactcatag
    26221 tcaaagatat tttgatagtt caatgattgc tgctgatatt tcttcgtcat ttcctcttct
    26281 tctagtacct tggcaacttg gctaatacaa aaatcgctat aagcatagtc taatgtgtga
    26341 ttgactgatt cgtggtagtg attaggaaca tagccatatg tcaagtaatc ttctgttcct
    26401 tggcgtccat aatttggatg tttgctttga accgtagcac ctttttccat tgccgttaga
    26461 aattcaggca tcaagtctcg tcggatatct tttacagcag catctgcaat gaccgcatcg
    26521 atcaatgtcc caggcataag tccacgttca tcaggagaca gccattttgg caaatagcca
    26581 ctttctctat agctgttcaa aaaaccttcc agcatttctt cgtatttttc tacagcaatc
    26641 aaagaataaa gaggataaac cgttttatac gtatcccaaa acccattatt tgtatagaga
    26701 acaccaggtt ttatagatcg actagtcgta tcatagtgga ttttttgccc actaggatca
    26761 atttcataaa atgtttgtgg aaataaaaac acccgatata aattatgata aaacgtccgc
    26821 aattgttgtt gatcatggtg tgtgagctgt atacgatcaa aataggactg ccattgttct
    26881 tgactgtgag caaggtaagt ctctttgctt tggttttgtt ctagttccag gttataagct
    26941 gcttgttccg agctgataaa agatgtccca aatcggatag tttgtttttt tgtctgttca
    27001 aaatacaacg ggatcgagtc gctatttttg ttggaaacag ctaactcatc aattgttaaa
    27061 ggatattcaa atctcaatat aaaataaaaa gaaaaatcag gatcttctga accagcaaaa
    27121 tgtgtgattt ttccttccac tgtgtaagga tctataacaa agagctgatg gcgtcccgga
    27181 aaggataaaa gtaaaccgct gtcatttttg ctgtaatcaa tagataagat tccgccatac
    27241 atgctaggta tcaatgtaga acagatgcca taacgtaatt gacagatcga cagatgagtc
    27301 gggcaaaata tactttcttc tgggcggtaa gaactttgag catggaaaag ggtgttctcc
    27361 tgtagttttc cattgatcgg cgtaaacagc atatgactga aatcacccat ccaaggactt
    27421 ggttgatggg taagacggta gccttgaaaa acgcggtcat caggatgaaa ccaccagctg
    27481 ccgttttgat ccgtagtttg cggtgcaaaa taattcatcc caaaaggaac gccagtataa
    27541 ggctggcagt tcccattaga gaaatttggc tgattcgctg ttgcatgtct agtatctatt
    27601 tgaccgatat tcattttatt tctcctttgt tgattgtctt ttgatgaatg aaactggaaa
    27661 tttactatcg gatggtcgat cttttgtctc taaccaagtg accaaagcat ctcccgcaag
    27721 cttacctaac tgttcaaaat cttgtgcgat ggttgtcagt ggcgggtcaa caagagctgc
    27781 tgcttggata tcatcaaatc cgataattga aaaatcttca gggatatgat aaccatgctg
    27841 tctcaagaga ttcatggtct ggatagcgac aacatcgttt tcacaaacaa aagcagttac
    27901 ttcatactta gtgatataag aaagcaattc atctaatgtc gcgcttttgt catccagtga
    27961 agtgtggaaa gtcaaaccag ctttgtccaa tgcttcaagg taaccaaggt aacgctgtcg
    28021 aacggattgt ggatgagtct gcgtaccaaa caaataagcg atttttgaat gtccctgttc
    28081 gatcaaataa gaggcagcaa gcgcaccgcc ttggaaatta tcggaaagaa cggtaggaaa
    28141 aggtaactca tagatttttt tatctaatat gacgacaggg aattctttca atgaaagctc
    28201 aaaaagtaag tcaagatgtt gttctgtatc aagagcataa taaatcaatc cgtcaaattc
    28261 attgattagc tcttttgctg ttttattttg tagaaagtca agcgtcgtca tcatgacatc
    28321 aatctgttta tcttgcatca cagggttcaa tcccttgcta aaatcaccaa cagacaaatc
    28381 agcgacatat ggaagaagaa acagaatccg tcgtgttttt gtaggagaag caagaatctg
    28441 ttccggagaa gcggatttaa cgaaactgcc tttcccgcga acgcgataaa taagtcctaa
    28501 ttgctctaat tcagtcaatg cacgtttgga agtgattctg cttactttat aggtacgtga
    28561 aagttctttt tcagttggta gttggctgtc tacaggtaat tcaccagtta aaatagccgt
    28621 ttgcaaatca ttaaatattg tctggtacaa tggttgagtc atataaacac ctcttaatga
    28681 tatatcatat tcaaataata ccataatgga tgaaaaagta aataaaaaga tattttttga
    28741 aagcgtagac ataattggct gtatgaagta taatctttaa agatatatca aatagaaaga
    28801 aggttttatt agtggtttat aaagatatac caaaatcggt gcagaaattt atggaagata
    28861 tcacacaaaa atgtggggat atccatgcgg actgggcaaa gaattttaca gcagcatttg
    28921 ccaatactct gttgacaaca gtcaaaaagc aaaatgacgg aaccaccttt ttattgacgg
    28981 gagacattcc agcgatgtgg ttaagagact ctactgcaca agttcgtcct tatttagtga
    29041 ttgcaaaaga agatgaggaa ttagctgaca tgatcagcgg gttagtcaaa aagcaatttt
    29101 tttatatcaa tatcgatcca tatgcgaatg ccttcaacga agaagctaac ggtgctggcc
    29161 accaaacaga tcatacaaaa atgaacgatt ggatctggga aagaaaatat gaaattgatt
    29221 ctttatgcta cccaatacag ttggcctatc ttctttacaa aaatacagga aaaactgatc
    29281 aattcaatca atcttttatt gaaggtatca aaaaaatctt ggacgtgttc aaaacagagc
    29341 aagatcatga acactctcct tatacatttg tacgagacac gacaagaaaa gaagatacgc
    29401 tgatcaatga cgggcgaggt gcaaaaacag cttttacagg aatgacatgg tctggtttcc
    29461 gtcctagcga tgatgcttgc cgttatggat atctggtacc atccaatatg tttgcggtgg
    29521 tcgtgttagg ttatgtggaa gaaatttttc aaacgatact cgatgataaa gaaattgtac
    29581 agcaaacaca gaaattgaag aaagagatcc aagaggggat tcagaatcac ggacgaacgg
    29641 ttaatcaaaa agggcaaacg atttatgcat atgaagtaga tggaatggga aacgccacag
    29701 tcatggatga cagtaatgtg cccaatctga tcgccgcacc ttacttggga tattgttctg
    29761 aacaagagga agattatctg caaacaagaa gaacattgtt gagtaaagaa aatccatatt
    29821 tctatgaagg aaagtacgca aaaggaattg gatcctccca tacgccagaa aaatacgttt
    29881 ggccgattgc attagctatg gaaggaatga caacgtcaga caaatctgaa aaagagcgga
    29941 tattgaattt acttgtagca actgacgcag gaacacacct tatgcacgaa ggattcgatg
    30001 tgaacaatcc tgaaaattat acgagagaat ggttttcttg ggcaaatatg atgttttgcg
    30061 aattagtcat ggattacttt gatattagag tagaaaagta ggaggtatcg taaatgaaaa
    30121 agaaagtata tatcatttct catagccact gggatcgtga atggtacatg gcttacgaac
    30181 agcatcacat gcgactgatt gaattgatag acgatctact agagctattt gaagttgatc
    30241 cgtccttcaa cagttttcat ttggatgggc aaacgattat tttagatgac tatcttcaag
    30301 taaggccaga aaaaagacag gcaatccaac aagcaatcaa tgaaggaaaa ttgagaattg
    30361 gaccatttta tatcttgcaa gatgattttc ttatcagctc ggaatcgaac gtgagaaata
    30421 tgctgattgg catggaagaa agccgtaaat ggggaacccc tgtcatgtta ggctattttc
    30481 ccgatacatt tggaaatatg ggacagacgc ctcaattaat gaaacaagct ggtatttcag
    30541 cagcagcgtt tggccgtggt gtaaaaccaa ttggctttga taatcaagtt ttggaagcag
    30601 aaaactattc ttcccaatac tcagagatgt ggtggaaagg ccctgatcaa acagcaatct
    30661 ttggtctgct atttgccaat tggtatagca acgggaatga gattccagta gaaaaagaag
    30721 cagctcttgc cttttggaag caaaaattag cggatgcaga acaatatgcc tctacgaacc
    30781 acttattgat gatgaatggt gttgatcatc aaccggtaca aaaagatata tcaaaagcaa
    30841 tccatctcgc aaatgaactg tttccagatt acgagtttat tcattctaat tttactgact
    30901 atctggaagc agttcaaaaa gatgtgccag aagacttagg ctcagtagaa ggagaattga
    30961 caagtcaaga aacggatggc tggtatactt tagccaatac agcatctgct cgggtttact
    31021 taaaacaatg gaataccaag gtccagcgac agctagaaaa tattacagaa ccacttgcga
    31081 cgatggctta tgaagttagt ggaaactatc cccatgatca attagattat gcttggaaga
    31141 cattaatgca aaatcatcct cacgacagta tttgcggttg ttcagtcgat tctgtccatc
    31201 gggaaatgat acctcgtttt gaaaaagcag atgaagttgg aaagtatctt gcacaagatt
    31261 ccttagaaca gctgaccgca gcaatcgata cgaccggatt cccaaaagac agctttccat
    31321 ttgtcattgt aaatactgca ggaatggata aaactggtga agcagaaata accatcgaat
    31381 tagaaagaaa aagattcgct gaaggaatac ctgaacaact atatcaagag ttagaaaatt
    31441 tgcctaaacg taagtatcat gtagaaacaa aaagtggggc cactatacca gctattttat
    31501 ctgaagaaac tgtccaattt ggttatgatt tgccgaaaga ccgtttccgt gtgccatata
    31561 tggcaagaat gatcaaagtg actttaccat tagaaaacat gccagcattt tcttgggaaa
    31621 cattcgcgct tgtagaaggg gaagcgaaag cagaagaaaa ggaaacaatg attcatcaga
    31681 gtggacgaat catcgaaaac ggccctctac atttgacgat tgaaaaaaat ggtaccatta
    31741 caatggaaga tcgcaaaaac aaacgaaagt tgaataatct gcatattttt gaagatatcg
    31801 gcgatatagg aaatgagtat atctttaaac aacctttctg tgatcagcca atcctatcca
    31861 gtaacaaaga aaactctgaa gtaaaagtat tagtagatac accggaaata gcaaaaataa
    31921 gtattctaca ggaaatggaa atccctgttt ctgcagacga gcgtttagaa aaagaacagc
    31981 agatggttgt ggaatttcgt tatcgaaaag cagaacgatc caaagaaaaa cgaatccttc
    32041 aaataaaaac aatcatgaca atcagaaaag atagtaaaaa aatcgacttt gaaacgactc
    32101 tcgacaatca gatgaaagat catcgcttac gtgtcttgtt tcctacaaag ttacatgtgg
    32161 aacatcacga agcagatagt atttttgaag tggtgaagcg cccaaaccat gtgtctaaaa
    32221 gttgggaaaa ccccacgaat ccgcaacatc agcaagcatt tgtcaatatt catgatgaag
    32281 agtacggtgt aacagtcgga aactttggtt tgaatgaata tgaagtaaca gaagacggac
    32341 aaatcgcagt gacgctactt cgcagtgtgg gagaactagg cgattgggga tattttccaa
    32401 caccagaagc acagtgtcta ggtgagcatc ggttcaatta cagcattgaa ctacatggac
    32461 cagaagaaaa attctctact tatttgcatg catacgcagc acagattcct ttttccactc
    32521 aacaaatcaa acatcatgaa ggaacattga tttcaaaaca acaatattta acgataaaaa
    32581 gtgaaacgtt tgcaattaca gctttgaaac gcagcaagtt tagtgataaa gtagtagtaa
    32641 gaggtttcaa tatgtctagt catttagaaa aattggaaat aactaaagac aatggaaaaa
    32701 ctgtcatatt gaatctgctt gaagaaccaa cgaagcaagc ggttgtccca ataatccaac
    32761 cttatgagat tcggacaatc ggattcgagg aggaaaacga atgtatggcg gaatagaagc
    32821 aggcggaaca aaatttgtgt gtgcggtttc agatggtgga gaaattttag aaaaattaag
    32881 tatccctacc acaacaccag aagaaacatt ggatcaggta tttgaatttt ttgatcgtta
    32941 tgaattgaag ggaatcggga ttggctcatt cggcccgatt ggtatcgaca aaacaaaaga
    33001 taattacgga tacgttttgg caactcccaa aaaagggtgg gcacaattcg attttcttgg
    33061 cacaatcaag gaacgctaca atattcctgc tgcttggaca acggatgtca atgctgctgc
    33121 atatggggaa ttgaagcaag gagcagcaca aggaaaaaat agttgtattt accttaccgt
    33181 gggaacagga atcggtgcag gtgtcgtgat caatgaagaa attttttcag ggatcgcaca
    33241 tcctgaaatg ggacatattt gggtgaaacg ccatccagat gaccattatg aaggtacttg
    33301 tccttatcat aaagattgtt tagaaggatt agcagcagga ccgtctattg aggcacgcac
    33361 agggatcaaa gggcaagatc tgccgcaaga tcatcctgtt tgggctattc aggcttatta
    33421 tattgctcaa gcactgatca actatacgtt gacgttagca cctgaaaaga ttattttagg
    33481 cggtggggta atgaatcagg atcatctcct tcaaaaaatc cgtcagcaat ttgttgaatt
    33541 gatgggtggt tatatggaaa cacctcaagt aagtgaatat atcgttcgtt ggggactgcc
    33601 gaatgaaagt gggattatcg gtagtttgct gttagcagaa cacgaacatc aaaagtaagc
    33661 agacaaatta cacaaaaaga acagagtctt atcattatct gctgataaga ctctgttctt
    33721 ttttatttct attcccataa tccaggtaaa gctttaccga ctattttttc ttgagtaaat
    33781 ggatggatca tgtgcagttc taatgcatgg agcatcagtc gttcagctgt tctattttgg
    33841 tagagagggt ctccaacgat cggatgtccc ttatgagcta aatgaactct gatctggtgt
    33901 gtacgtccgg tttccaaaac acaatagact tgtgtttgtt cagaaagtac tttgttgaca
    33961 gtgacatgtg tcaaggcgct ctgaccattt ttatcatcta cgaccctttt tctccgatca
    34021 tggcgatctc gtccaatttt attggtgatg gtcatatctt tggcaatttc tccccatacg
    34081 atggcttgat atctccgata gatcattttt tgttccaaca ttcttccaag tatcggcaag
    34141 acaaatggat tttttgcaaa taaaacggca ccgcttgttt ctttgtccaa gcggtggaca
    34201 acgtaaggtc gttgctgttt agtgtcgaga taagcagcta aatgattgag taaggtactg
    34261 ttttctgtcg gttcatttgg atgagtcttc attcctgcag gcttatccac tataatcaaa
    34321 tgctcatctt catataaaac acggatgtgc ttcgcatctc ctaattgaac ttcttgataa
    34381 ttataatctg tttcttcgaa taacaatgtg atctggtcat tgccctttac ttcatgatgg
    34441 aacaaagcag gttgttgatt gatccaaacg tttttacgta tacgcaaaaa gtgtcgaacc
    34501 tttctaggaa ctaaccattc cttttcaagc aagtcgcgaa ttgttgtttc aggatggttc
    34561 ataggtaaag tgatcgtgat tttcacaagt gtactccttt cagtgcaatt gcagactaat
    34621 tgtaccattt ttaaaacata tttaagaaaa aacttacatt tttctgttca ttcttatgtt
    34681 actctacttg cactaacgta aaaaaacata ttgaactaca acttaagcag gaagaaaaca
    34741 ctctttcact atggtaaaat aagacgattg aatcaaatag acaggagaag aagcaaatgg
    34801 attttcaaag cattttagaa aagataaaag ctgctttgat cagtttttgg cgttggatca
    34861 gaccatatct cggacaattc catcgatggc gaaagcgcat ctggaaaaaa tatcatgtga
    34921 acaaattgat tttgctgatt ggtttgatct gtgtcttagt gacaagtatc tacctcttta
    34981 ttttagcaaa acaagcaaat gtcgaaacgt taaaatcagg attaagccaa tcgacagtca
    35041 tttatgataa gaaaaacgaa gaagctggca cactgtacgc ccaaaaagga acatatgtag
    35101 aattaaacgc gatttctccc tatatccaag atgcagtaat ctcgacagaa gataggaatt
    35161 tttacgagca tcacgggttt gatatcaaag ggattgcccg tgcagcagtc cgaatggtct
    35221 tgtctggcgg aacatcaggg ggcggaggaa gtacgatcac tcagcagctt gccaaaaatg
    35281 cgtatctgac attagaccaa acgtttgatc gaaaagcgaa ggaattattt ttggcaatcg
    35341 agatcgaaaa gaagtacagc aaagaagaaa tcttgaccat gtatcttaac aatgcctatt
    35401 ttggcaacgg ggtatggggt gtccaagacg cttccagaaa gtattttgga gtagatgcaa
    35461 cgaatgttac gctgtctgag gctgctactt tggcaggaat gctgaaaggt cctggtattt
    35521 ataatccgat cgatcaccca aaaaatgcga atgatcggaa aaacacagta ctaagtgtaa
    35581 tggaagaaaa cggaaaaatc acaaaagaac aagaacaagc agaaagccag acagatatca
    35641 gcgcgtatct taatgacaca tacgataatt cagattctgg ttatcgttat ccttattatt
    35701 ttgatgcagt gatcgatgaa gcagttaacg agtatggtct tgatgaagaa gatgtcatga
    35761 ataaaggcta taaaatctat acagctttag atcaggatta tcaaaagcaa ttagaagcta
    35821 cttatcaaaa cagtgcatta ttcccagcaa atgcttcaga tggtgccatg gtccaatctg
    35881 cttcagtagc tttagatccg aatacaggtg gtgtacaagc actagtagga cgaagaggag
    35941 atcatgtctt tagaggattc agctttgcta cacagatgcg tcgttcacca ggatcaacga
    36001 tcaaaccatt aagcgtatat acaccagcac tggaagctgg atacaagcct tccagtatct
    36061 tgaaagacga accgcaaagt tattacgatg cgcataattt tgacgggaca taccaaggag
    36121 aagtaccgat gtatcaagct gttgcacaaa gtttgaattt gcctgctgta tggcttttgc
    36181 atgagatcgg tttacaaaaa ggatatgata aagcagaaga attcggcctg cctttagcaa
    36241 aatctgataa gtattatggc ttagcccttg gtggactgga aaaaggagta tctccattga
    36301 tcatggcggg tgcttacagt gcatttgcca atgatggtca gatgtatacg ccgcatctta
    36361 tcacgaaaat cgttgattcg actggagcgg tgatcgtcga taacacaaac aaaaaaccaa
    36421 aacaggtcat atcaaaggaa acagccgatg aaatgaccag catgcttctt ggtacatttt
    36481 caaatggaac agcggctgct gcgaatcctg caggttatac gatagcaggg aaaacaggaa
    36541 ccacagaaac taatttcgat gccacaaaag caaatgacca atggatgatc ggttacacgc
    36601 cagacgtagt gatttcgaca tggatgggtt acgaagaatc cagcaagctg cactatttgg
    36661 aagggacaag tggaaatgtt gtcgggaaag tcttcaaatc agcggcagaa ggcatccttc
    36721 cgtatacttc aaaaacagga tttagtgtag cagatgccta tgccacagga ggacaagtgg
    36781 tagctgcaga tcaagttgga aacacaggaa attctaatgg agagtctgga aattggcaag
    36841 ataacttgaa tcaatatggt gaacaagcaa aagaagggct gaaaaacttc ggcgatatgg
    36901 tgaaagaagg cgttcaggga attggtgaag cggctaaaga cctctggcgg aaatatcaag
    36961 gagagtagcc cctttgaaaa cgaataaccc catgttacaa tagagagtaa ctactaaaag
    37021 aaaagagggc ataacatgtc aaacatctac gatagcgcga atcaaatcga acgtgagatc
    37081 cgtgaattac cagaattcaa agcgttgaaa gaagcgtacg agaaagtaca agcaaacgaa
    37141 gaagcacaca aactgttcaa agacttccaa gcgatgcaaa tcgaattaca agaaaaacaa
    37201 atgagtggac aagagttcag cgacgaagat gcaatgaaag cacaagaaat ggctgtgaaa
    37261 atccaatcag aagaagtaat caatgacttg atgcaaaaag agcaaggctt cagtacaatc
    37321 atcaatgact tgaaccgtat catcatgaca ccagttcgcg acctttacag cgaataaaac
    37381 tattttatga aacaacagag ggtgtgaaag agacgttcgg ccttgagcga tgacaaaatt
    37441 gcttcttatt gccgataggc tgtctcttga acaccattta ccaagaggtc gtgagcctac
    37501 cgtttaactc tgagttacaa ggaacaattt caaaaaacag cttctcatgt ttgatgacta
    37561 ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tattcagaaa taggagctac
    37621 gacaaaagtc ttgtcatagc tcctattttt tgtcattcat ttttcaagcg gtagaaatag
    37681 cctttttgag ctaacagttt ttcgtaagta cctatttcaa cgatttttcc ttcatcgatc
    37741 acaacgattt tatctgcctc tttgatcgtt gaaagccgat gagccacaat aaaagtagtg
    37801 ggtgtttctg tcaggtaatt cagggcttgc tggatttttt tctctgactg gttatctaaa
    37861 gctgaggttg cttcatctag caaaatgatt tttggctgac gtatcaacgc tcgtgcaatg
    37921 gagatacatt gcttttggcc accagacagc ttgttgccat gttcgcctac taaggtatcc
    37981 agaccatcag gaagctggtc aatcagtgaa gaaagttgcg cagcttcgat gacttcatct
    38041 aaacgttctt ttgatacgtt tttcaagcca taagtgatgt tctctttgat cgaagcggag
    38101 aacaacaaag tggtttgagg aacaacggct aaataatttc gcacacttcg catatccagt
    38161 tcatccaaag gctgatcatc tagaaaaatt tttcctgaag tcggctggat aaatccaatc
    38221 aataagttaa ggagtgtaga ttttccagag ccagatggcc caacaaaagc aattgtttct
    38281 ccagctttca cctctagaga aaaatgatcg ataatcggtt tgagtgagtc cggataaaga
    38341 aaagaaacat cctcaaaacg aaaatttcca tccaaatgtt ggattttttt cttaccgtga
    38401 taagtttcta catcgccagc aatcaacagt tcgccaatag aagtgataga ttccatgcct
    38461 tttgaaatcg taggaatcag cgtgatcatt ccagaaacgg aattgacgat cgtcgtgaaa
    38521 tacgtctgat acatcacgac atcgccgggt tggatgcgcc cttgtaaagc taatatccca
    38581 gtgaaaatca aacaaatcag ctgaaatatc tgaaaactag cccagctgac tgatccgaaa
    38641 acagattgca ggatatctaa gtggaatcct ttttcataga tttgagtaaa atgcttattt
    38701 aagcgagttg tttcgtgttt ttctaaccca tgagctcgag caatcggtgc taattcgatc
    38761 acttcgatca cttttgcaga agtctcttcc atttcttgcc tgaagtctga gttggactgt
    38821 ctgatccttt tacggaaaaa gagagtaacg aatgcggcga gaggaacagt caataagaaa
    38881 aacatgaaaa caacaggact tttataaagc gtgataccta aagctacgaa taaattgaca
    38941 gcgatattta atccgctgac aaatacttga gaggacaacg tttggatagc ttctacatca
    39001 cggatgatct ttgactggat tcgtccagat tgcaattctt tttgataagg aatagatagt
    39061 tcttggattt tcttgataag ggtcgctctt agtccagctt ctacttgacg gatcgaagtg
    39121 ctatggtatt ttacatgaaa ataattcgtc aatatatttt gaccaatcag acaagccatc
    39181 aaaaaagcgt tgaaataaat ggcctgcaac tgagaagaat ctttcgatga agctgcattg
    39241 attacattgg caatagcgat cggcattaac caagatggaa gatgtttgat aataaaaaag
    39301 agtcctgaca tggcaaaacg ccaataattt ccctgataaa gatgcagcaa tgtctgaaaa
    39361 gcatttgcat gatatttttg ataacttttc tgtattcgtt cctccataat cttctcctta
    39421 acaatcttgg taaataaact gcaagtgttc tattgcttca ggaacataaa tctcaggagt
    39481 agcttcaaga gtggcgtaca ttagaggacg ctcatatttg atataatgca gtaccgggcg
    39541 gaaatctaaa cagcccttgc caacaggtac catgcggatc tttccatttt ccatgatata
    39601 gtctttgaga tgaagcgctg taatataacc atgtaaattg tccaaggcac ctcggatcac
    39661 tttttcctga tcagcatgat tttctttatg catcaaattc gcacaatcta aaatgatttt
    39721 taaatttggt gaagctactt catcgattaa acgtttagct aactgatagt tatagattgg
    39781 atgattgatc cctgcttcaa cagcaacagt tacgccaaaa ttttctgcat gagcgactaa
    39841 ttcaatgata gaatttctgg cgatctgata agcttcttca gtaaaatttt cttttgtgta
    39901 accgacagag acactaccgg tttccgtagc aatcatcttg gcttgatagg cagagcacag
    39961 agcaatttgc tgtttgaaag ttgccaatgc ttgtgcacga acttcttgat ttttgctgga
    40021 taggttgata tagcatccta aaatggaaag ttcgatattg tttttttgga agtattcacc
    40081 aaaataggca gcactcccaa gtgtcatgtc agttaattta agttgttcag gaaatgcttt
    40141 ttgtggtgca aattggatat gggacaagtg aagctgtgat actttttgaa cgatttcttc
    40201 acgatttcga acatctaaat cgtgggcacg gatgcctaaa tttaatggaa acatctcaaa
    40261 ctcctttttg taaataatta gcaagaggca gacgggattc ttttatcaat tcaacaatta
    40321 ttcgggcaac tttatcagcg ccttctggac taaagtgggt attgtctttt agtccttctg
    40381 ggtaattttt acaagtatca ggcataagat gcaggtagaa tttttttgtc tcttcttttg
    40441 agaaggtctg gaacaattct tgtgatcttg agaacacatc taaaagagga atatggtgtt
    40501 tctcagcaaa tgcacgcatc gcttttggat agtctccaag tatatctgga ttcaatgttc
    40561 cgttttctaa gtaatctcgt cgcgtcacag atgtcagtaa gaccatttgg acgttcgctt
    40621 tttttgcgca ttcaaaaaat atctgcagat tttttaaata gctaccgaag ggaagcgtac
    40681 ctctttcttc ttggattttc tggtcgttat gtccaaattg tacaaataag aaatcattcg
    40741 gctgaaactg tgaagctaga cgggctaacc ttccttcctg gataaatgat ttggtgctgc
    40801 gtccattctt tgcttggtta ttgatctgga tctcgggtga aaaataagca gagatttttt
    40861 ctccccaacc tgtctcggga cgcttgtttt catctttttc tgccgcagta gaatcaccgg
    40921 caatatggat agtaggcatg ttatctcttc tttcataaag gtttataggc aatgaagcag
    40981 aacagcaaga gaagaagacg gagcaa
//
LOCUS       DAJSBN010000023        40867 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJSBN010000023 DAJSBN010000000
VERSION     DAJSBN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.023"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(153..935)
                     /locus_tag="ONT86_001329"
     CDS             complement(153..935)
                     /locus_tag="ONT86_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352133.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1000..1836)
                     /locus_tag="ONT86_001330"
     CDS             complement(1000..1836)
                     /locus_tag="ONT86_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2352134.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1854..3263)
                     /locus_tag="ONT86_001331"
     CDS             complement(1854..3263)
                     /locus_tag="ONT86_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2352135.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3277..5100)
                     /locus_tag="ONT86_001332"
     CDS             complement(3277..5100)
                     /locus_tag="ONT86_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2352136.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5943..6269)
                     /locus_tag="ONT86_001333"
     CDS             complement(5943..6269)
                     /locus_tag="ONT86_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352137.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6315..6677)
                     /locus_tag="ONT86_001334"
     CDS             complement(6315..6677)
                     /locus_tag="ONT86_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR2352138.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6714..6929)
                     /locus_tag="ONT86_001335"
     CDS             complement(6714..6929)
                     /locus_tag="ONT86_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2352139.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7056..7391
                     /locus_tag="ONT86_001336"
     CDS             7056..7391
                     /locus_tag="ONT86_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2352140.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7467..9464)
                     /locus_tag="ONT86_001337"
     CDS             complement(7467..9464)
                     /locus_tag="ONT86_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352141.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9562..9837)
                     /locus_tag="ONT86_001338"
     CDS             complement(9562..9837)
                     /locus_tag="ONT86_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2352142.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10069..10803
                     /locus_tag="ONT86_001339"
     CDS             10069..10803
                     /locus_tag="ONT86_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2352143.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10842..11984
                     /locus_tag="ONT86_001340"
     CDS             10842..11984
                     /locus_tag="ONT86_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2352144.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12079..13470)
                     /gene="sufB"
                     /locus_tag="ONT86_001341"
     CDS             complement(12079..13470)
                     /gene="sufB"
                     /locus_tag="ONT86_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR2352145.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13475..13945)
                     /locus_tag="ONT86_001342"
     CDS             complement(13475..13945)
                     /locus_tag="ONT86_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCR2352146.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(13932..15167)
                     /locus_tag="ONT86_001343"
     CDS             complement(13932..15167)
                     /locus_tag="ONT86_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR2352147.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15164..16450)
                     /gene="sufD"
                     /locus_tag="ONT86_001344"
     CDS             complement(15164..16450)
                     /gene="sufD"
                     /locus_tag="ONT86_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR2352148.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16463..17236)
                     /gene="sufC"
                     /locus_tag="ONT86_001345"
     CDS             complement(16463..17236)
                     /gene="sufC"
                     /locus_tag="ONT86_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR2352149.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17433..18281)
                     /locus_tag="ONT86_001346"
     CDS             complement(17433..18281)
                     /locus_tag="ONT86_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR2352150.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18305..18991)
                     /locus_tag="ONT86_001347"
     CDS             complement(18305..18991)
                     /locus_tag="ONT86_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2352151.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(18984..20018)
                     /locus_tag="ONT86_001348"
     CDS             complement(18984..20018)
                     /locus_tag="ONT86_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2352152.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20081..20337)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20413..20751)
                     /locus_tag="ONT86_001349"
     CDS             complement(20413..20751)
                     /locus_tag="ONT86_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCR2352153.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20753..21109)
                     /locus_tag="ONT86_001350"
     CDS             complement(20753..21109)
                     /locus_tag="ONT86_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCR2352154.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21115..22302)
                     /locus_tag="ONT86_001351"
     CDS             complement(21115..22302)
                     /locus_tag="ONT86_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR2352155.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22493..23338)
                     /locus_tag="ONT86_001352"
     CDS             complement(22493..23338)
                     /locus_tag="ONT86_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2352156.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23572..24537
                     /locus_tag="ONT86_001353"
     CDS             23572..24537
                     /locus_tag="ONT86_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCR2352157.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            24914..25957
                     /locus_tag="ONT86_001354"
     CDS             24914..25957
                     /locus_tag="ONT86_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCR2352158.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26019..26483)
                     /locus_tag="ONT86_001355"
     CDS             complement(26019..26483)
                     /locus_tag="ONT86_001355"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR2352159.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            26729..27676
                     /locus_tag="ONT86_001356"
     CDS             26729..27676
                     /locus_tag="ONT86_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2352160.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(27887..29959)
                     /locus_tag="ONT86_001357"
     CDS             complement(27887..29959)
                     /locus_tag="ONT86_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR2352161.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(29892..30269)
                     /locus_tag="ONT86_001358"
     CDS             complement(29892..30269)
                     /locus_tag="ONT86_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCR2352162.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30398..30976)
                     /locus_tag="ONT86_001359"
     CDS             complement(30398..30976)
                     /locus_tag="ONT86_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2352163.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31108..32400
                     /locus_tag="ONT86_001360"
     CDS             31108..32400
                     /locus_tag="ONT86_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR2352164.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32460..33578)
                     /locus_tag="ONT86_001361"
     CDS             complement(32460..33578)
                     /locus_tag="ONT86_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCR2352165.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33592..35013)
                     /gene="arcD"
                     /locus_tag="ONT86_001362"
     CDS             complement(33592..35013)
                     /gene="arcD"
                     /locus_tag="ONT86_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCR2352166.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35259..36380)
                     /gene="mnmA"
                     /locus_tag="ONT86_001363"
     CDS             complement(35259..36380)
                     /gene="mnmA"
                     /locus_tag="ONT86_001363"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR2352167.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            36784..37383
                     /locus_tag="ONT86_001364"
     CDS             36784..37383
                     /locus_tag="ONT86_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCR2352168.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37521..38807)
                     /gene="efmA"
                     /locus_tag="ONT86_001365"
     CDS             complement(37521..38807)
                     /gene="efmA"
                     /locus_tag="ONT86_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HCR2352169.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39078..39422)
                     /locus_tag="ONT86_001366"
     CDS             complement(39078..39422)
                     /locus_tag="ONT86_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCR2352170.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39559..40704)
                     /locus_tag="ONT86_001367"
     CDS             complement(39559..40704)
                     /locus_tag="ONT86_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR2352171.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11724 a         8608 c         7165 g        13370 t
ORIGIN      
        1 tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga aaaaaaatac
       61 ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata acaacggggt
      121 agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt aaatacttcg
      181 ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatctttttt cccattttca
      241 agtaaccgaa gtggaatgtc tttaccaact tgctctgtca ctgcattact gatacctgtt
      301 agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt atcagtattt
      361 atcgtgattc ctttatcttc atttacaagc ttccaagtag gtggtatagc tgtgtatgtt
      421 tcgatgaatg aatcaattct ttcatctatc gtcttcccag tatctacttc tgtctcatta
      481 ctcttagatg aatcgattct ttcatccatc gtctgctccg taactgcttc tgtctcatta
      541 ttctcaccgg aaactctctt cacacccaaa cttatatatt gtccatcttt agtgtgcata
      601 gttaaaattc ccacgctttt actaataaat tcactataac cagttctttc aatagagact
      661 tcaatgtatt tcacactatc atactcaaaa gaaaaataga catgtaaaag cattttgttg
      721 tttacatcta cataataatc ctttgactct acttgagacc ttcctatacg gaatacagct
      781 ttactgtctg atatctctat accagcatcc ttattattaa tctgccaatc tccaagtagc
      841 tcttctggca agctagtagt ttcttgtgaa cactttacca cttcactggt agaggtcgaa
      901 acattcacct ccgcactgtt agaagaattt tccaaggaat caggagcctt agcacaacca
      961 acaagtcttt atatattgtg cagccgatat tttcttcatt cagtacaccc ttgaaatatg
     1021 cagtgttaga taaagcagtt cgtcctgatt tgctgaggca cccatctctt cttgaacgta
     1081 ctccttgatt ttcaaggccg cccgatactc tttcggaaaa cgctgcttca ctgaagccag
     1141 caaaaaatca tccttctccg gcaactcttc cttcttaatc attcgggcaa taaaaaactg
     1201 taaatgcgtc acaaatctta agtaaagcat cgaatcttta tcaacctttt tttcgggaaa
     1261 cagatatcct agcatagcca agatatcctt tagtgctctt accgagagca tggtttcttc
     1321 catatttgct tgttcacttt gggcatttac aatgtgaaac gcaatgtttc cagcctcctc
     1381 ttctggcaac tgaacattca tttttttgtt gatcaattgg atagcatagc agccgatctc
     1441 aaattctttt gggtaatact tttgaatttc aatcagcagg cgattttgaa aaacaatgcc
     1501 tttttgactg cgttcaatgg catgcttcaa gtgatcaacc aaggtaaaaa atagactttt
     1561 attcagctta aagtcaaact tggcgttggc ataatcgata attcgcttga cgcaaataat
     1621 tagatcatcc ggagcagcct caaacagttt aatgtaatcc ttttttagag catcagaaag
     1681 tgaatctaat ttaaatactt tttggatttt gtcagccgga accaaatcgc ctttctttag
     1741 ttgaaatcca atccctttcc ccatcaaaat tacttctttg ccttgcttat ctaagcaaag
     1801 aattaaactg ttgtttaacg ttttaattac tctcattaga ttcaccgttc tctctaagct
     1861 aagccatttc ttgcgattac tttttgatac caaaagaagc tttctttttt tattcgtttt
     1921 aattctttaa gattctcatc ggttcggtca acatatataa aaccataacg cttattgaag
     1981 ccttgatggg tactaacaac atcaatcgct gaccaaggta aataaccaaa aacatctaca
     2041 ccatctgtta aagctgcttg aatagccaga atatgtttgg ctagataatc aattcgatcc
     2101 tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg ccccatagcc attttcagta
     2161 attaaaatag gtaaatcata gcgatcgtac aatttcctca atgttaatcg tagtccagta
     2221 gggtcaacta cccagccata tggcgtttta tctacataag gattttcagc tgcccggtag
     2281 acaccttctt ctcccaacat gatctgttga tcccccgctc tagcggaaac atctgatccg
     2341 tcatttttgc tggcagcgat ggtggcagtt gaataataat taatcgcaat aaaatcgggt
     2401 ttaccggagg ataaaattgc ctgatcctct gcctcaattt taggatataa tccacgatca
     2461 ttcaaatagt tttcaaacaa ataattgtat tttccccgaa ctgctacatc taagaaagac
     2521 cagcctctca atgtttccca attatgggct gcaattgcat cttctggact ggaggtagct
     2581 tgatacatag ccgttaaatt gattgctgga ccgatttttc ctttaagatt caactgatga
     2641 tacaagtgaa ttactctagc ttgagctaat aaaacatggt gattaatttg aaagagttct
     2701 ttttttgaag gcagctctcc acttggtggc aaaccaatcg ctccaggatg aaggatcatc
     2761 gtgttttgct cattaattgt cagccaatag ttcaccttgt caccatactc agtaagcaat
     2821 acctgtacat agtccacaaa ataatcgata gttgctcgat ttaaccaacc gccctgctcc
     2881 tctagaaaat aaggtaaatc gaaatggtac atggtgacaa tcggttcgat tccctgattc
     2941 aggcattcat caattacttg atgataaaaa gaaattccct tttgattggt tttattattt
     3001 tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa aagcttttaa gcctaattct
     3061 ttaaacagct gtatatcttc tttatagtga tggtaaaaat ccgaggcaac ttcaaaatta
     3121 gctacccctt ctgtatgctg atacttgtca ataatagacg gttgtttccc atcctcctga
     3181 taggcaccct ctgcctggta ggcactaatg gaggcacccc ataaaaactt ctctggaaat
     3241 gctgaaagct gtttgtattg cacaaaaatc ctctccttaa ctaatttgca tcaatggttc
     3301 gtgttccttt aagcttccgc ttgcttcact aattcctgcg taattttcac tgttggtcac
     3361 taccacaatc actgtatcat cgtagccagc gtttctaatg gcagcgaaat cggcgttgat
     3421 taataattgt cctgctgaca cctgatctcc tgcttttaca aatacttcaa agggttcccc
     3481 ttttaaattc acggtgtcga tccccacatg gatcaataat tcaatacctt ctttagaacg
     3541 tagaccaatc gcatgtttag attctaaaat agtaatgact tctccctcaa caggtgaata
     3601 aatgctgttt tcttctggat gaatggctac gccctttccc aatagtccct tagaaaagac
     3661 agcatcgttc acttcagaaa taggacacac ggttcctttt accggatttt caacaatgac
     3721 tgcttcggct attgccgttt gctctgattc atcagaagta tcgtcaaatc caattagata
     3781 agcaatgata aaggttgcaa caaaggcgat tacacacgta ataattgcgt gaacaatatt
     3841 cattggattt tctccaataa aggctggtaa ggctgctaag ccaggggaaa caaaggcata
     3901 tctgactaaa ccgctcaagc cagcatacaa accagcaatt gcaccaccaa tcatggaagc
     3961 aactaatggt tttttcagct tcaaggttac tccatagagt gaaggctcag taattcccat
     4021 taaagcagtg atcccagtgg acacagctag ctgcttcaac tctttgtttt tagttttcgc
     4081 ggctacagca aaggtagcac ctgattgagc aatattagaa gccagcattc ctggcccatt
     4141 gatcatttca gaacccgtac tgctcaattg catagtagcg attggcgtca ttgcccaagc
     4201 tgtacccgta accaccaaaa atggctgcaa accacccatc aaaaatggaa ttaaccaagc
     4261 tgctttgccg ttaatccatt cagcccccat tgctataaga tcgttcagca acgttccaaa
     4321 cggtccaatg acaaccaacg ctaaaggccc cataattaaa aataccagca tcggcttagt
     4381 aaagaattta ataaacgaag gcgaaattcg atccgcaaag ggctctacat aggacatgat
     4441 ccaaacagta aagataatcg gtaaaactga tcccgcataa tctgcagcta ataccggaat
     4501 tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt gaaatatttg gatgaagcaa
     4561 aatgccagca acagtaatcg ccaaaattgg gttgacatca aactttatgg ctgccccata
     4621 ggctaatagt actggcataa aataaaaggc tgcatcagca atagtattca aaatgatgta
     4681 agtttgacta ctgtctgata gtacaccagt caaagtcaag acggccaaaa tggcttttac
     4741 catcccggca cctgttatag ctggaatcat cggtgtaaag gtggtagaaa tgacactaat
     4801 caatcttgaa ataatacttt cttgattgtc tgaagagggg ccctttttaa ttgaagttcc
     4861 cacttccttt tgaatttcct tgaaggctgt ttggacttca ttgccaatca ccacttggta
     4921 ttgtccaccg ctatccacca cactaattac accaagtgtt ttttctaagg ctttcgtatc
     4981 ggctttcctt ttatctttga gttcaaaacg cagtctggtg gcacaatggg ttaagctgac
     5041 cacattttct tttcccccaa cctgttgcaa tacatttctt cctacttctc tgtaatccat
     5101 tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa ccacgcccca atcaacattg
     5161 aagtgttagt ttgtcttggt cttgcctgca taatcagtaa catgccaata acgttatttt
     5221 gctgtaatcc aataatagca cattatgata gcgcaatcaa cagggaattt aattttttat
     5281 cttttcgttt gaccgtatag ggtaatataa cagttagatc acatcaacta gggtatatct
     5341 gcgtatactt actgctaaaa taagtatatc taatattaac tataccagta aaaccattcc
     5401 tacataacca gcgggaaatt cccaaaatcg tgaaagcatc aaaaatgcat ataacggatc
     5461 acacaacttc aacaattctg ctcttgggaa aaattgatga atttggtgag taaaggatgg
     5521 aataaacagc attccaacaa tataggcaac aatagtgata cttgtttttt tggtaaaaat
     5581 cagtaagcaa tagccaattt caatcattcc gagatataaa ggtatcgata gaatgatttg
     5641 ggctagtatt cttaaaaatt aactagcaaa tgtttaaact gatttatttc taatctacat
     5701 gtagtaagta tttcaattcc accaattatc aataaaaata ccaaacatat ccataataat
     5761 tttttctttt tcatttcttt tatattgtta taacagtgaa taacataaaa attcccgaca
     5821 atcattgtaa tatttttttg tcacctgtat tagtatttaa tttataggaa actataaaaa
     5881 ctccctagtg aatttacacc tttgggagtt cctgtttgga cggtatgcat aatacattta
     5941 aatcaattta acattaacac ctcagcatat ggtatctcca catatatacg gtctttcttt
     6001 ggatagtact caatatttat gttgatagca cttccattgt ctaatatagc tttagcccgg
     6061 tatgtgttat ctccattttc atgagtaatc gtcacatcat cacatgatcg atttaagttg
     6121 tactgttctt gtaaaatctg tgttaccaat ggttttgatg acttctctaa tccagatgta
     6181 cctgtcattt ttgaaaaaat aatacctata atagccaaga caacaatacc tataatgaca
     6241 aacttcttgt tcatatttac tttattcaac tttttatctc ctatctccaa aataatttgc
     6301 tcttatccct cttatcagta cattgacatc cctaaaagta caccaaagat tgctccagct
     6361 acccctgcta tcatcaaaat cgtacccatt ttctcatctt tttctcgata taagtagccc
     6421 ataataacag caacagcacc aaatacaaca ggaataaatg ctaacgatac taccatagaa
     6481 atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat ttgttccttc
     6541 atttcattaa ttgtctgctc agtacttttc tttactgtgt ctgttccggt tgtctcatct
     6601 gtttcttgta gtgctccaca aaactggcaa tacttacttt catctgttat ttctttgcca
     6661 cattttctac aatacatgtc ttctcctcct aaaatcattc aattgcttat tagttaactg
     6721 tttactttat taaatacatc agaaaacagc tcattcattg gtacacttaa tatctcagaa
     6781 agcatcttca tttgataggg tttaggaatc gttctccctg tttgccacga acttacacat
     6841 tgctgtgtaa cattcatttt ttttgctaac tccgtttgag aaagtccttt ttcttttctg
     6901 tatcggctga gattacttac ttttttcatg attctcccct cctgttttat tatatacaac
     6961 aatatgatgt atttcaactc ataaaatata aaaaacttca aataccatta caaatacaac
     7021 aacttgttgt actatactga gatgggggtg tattaatgaa acagaaacaa tatgcaaagg
     7081 tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa agaattagct gaaaaaatct
     7141 tctctacaca acaggcagta gcaagatggg aaaattctgt aacagagcct aacctcgata
     7201 gcttaacagc tctttctaga gccctaggaa cccctgtaag ccattttcta gacaatgttg
     7261 tcgtagatta tgaggaagag tttttggctc tctatcgttc tcttagcact gaagatgcag
     7321 ttcgaacaat tgactatatg aagctattaa aaaggcaaga aaatgaacgc aatcagctat
     7381 tgaaagattg aaaaataaat aacccaccga actttaacgg tgggtgtatt gagatgaaat
     7441 aagattaaga gctatggata acctgtctag tttaatacaa ttgggtcttt tctcttcctt
     7501 actctccgtc catcatctct tgttacactt gtaaattgat acagaggaac tatcccagct
     7561 cctgataata tcaattcttt tattgtttca tctatccctg cccctttcgc taaatatatt
     7621 aaaaaactac tacttgcttt cacattgtat agtttcttaa tatccttaga agctcgttca
     7681 agagtgaatc cttgtttaac aaagtagcct ctgtttttta ttaaagcatt tttcacgctg
     7741 ttatccccaa cattacgtag gcgttcacaa acagtgttaa taagttcttt ctctgtcatt
     7801 agatatgtcc cttgccacga taagccctgt aaaacttcat gtgcttcatc taaaatcaat
     7861 tgtttttcct tgctgagttc tgatcgggaa ttttgaggaa atactctttt agcctctttt
     7921 tcgttaaaac atatcttaac ccattcataa gaaaaatatt gatatgtaaa atgaattttt
     7981 tccagaagtg gaaggatatc ttcattcaat ttttgaatca cagaagttgc attaaaaacg
     8041 tctaatcgat agacattgct tctcttatga ttaaagtttc ctaacgcttt ctcttccttt
     8101 aacctattta ggtattcgag catcttcaaa ggaacatctt catcatctaa tttttttatc
     8161 aatcctataa aagtcataag tgatagtaac ttattacatt tatctatgtc ataagtttta
     8221 ccatagtaag aactcatttt tttagctaat tgttcccccg acaaaataca agtcacactt
     8281 tccttactat catcaaaaaa attaacatca gaaagcaaga catcatagac acgtcgcatt
     8341 tcctctagat aattccttat tgtcctgaaa agtttgtctt tcattgattc aagagtgtca
     8401 aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg taggatacaa gtctaggttc
     8461 tctaatagcc aattaaatgt ttctacccga ctgtctttct ttaaaattag catcacaact
     8521 ccaataaaat tttcactgat atcacaatta ctggaatgac aataaaaaat ggaatgccca
     8581 gttttactgg taaaaatact agcagaggga ttatgatcgt tgtgaaacag acatcttaag
     8641 tttttacttg acttaagtaa tttattcata tcaatggtta acaatctttc ataaatctca
     8701 ttaaaagaca tgtcggagga atcaaacagg cagtctttaa accactgttt catttcttct
     8761 ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat tgactagttc atactctctc
     8821 cctgaatcac tttgacaatt tttagaacat gtcatggtaa aatcaatcgg aagctcagta
     8881 atatctaaca tgttgtcttc attaattatt atcactgcat ctgacttata tcctccaaaa
     8941 aacattctat tcgaagcatt tacattgtca tcacacccgg ggatgttctt tttcaagtat
     9001 tcgtaaacag caaccatttt accatagtca gtaattgctt cttttaatag aaagacaatc
     9061 ttgtattttt ctagattttc agagtaccga atactttttt gtatcgcaaa cgcagaatta
     9121 tgaactaaat tgatagataa aaccttttct acgcttaagt atctactatc atctttactc
     9181 aaatctacta ccttcccctt ctcatcttta acagtgttat cgacatccag aataattact
     9241 ctggtttcca caaaactctc cttgtcattg cctttattct ttgtcttgct tggaataaat
     9301 gattgtgatg aatttagtat ctctacaaac tcatctcttg taacttctct atagttatct
     9361 aaacacctat ctcttacatc agttttttct ttttttgtaa ctggcttctc taataatttt
     9421 tcttgatcta cataaattgg aatattaatc attgatgttt ccatttgtgt ttcctcctta
     9481 taaaattctg ttaaagacta aaacatacag ataataaaaa caaactcttt attactatat
     9541 gttttagtct tatttgttta ctcaaccttt ataccgttcc atgaaactat caatatcaga
     9601 ttttttaaat ctaatgaccg tatcaatttt tataacaggt aacccgtatt gagaaacata
     9661 ttttctaatc gttcctactg atacacctag atattcactt gcctgctgat agtccatata
     9721 ctctagagtg actccagaaa taatgtcttc tagcataggc tttagcactg taattatctc
     9781 tttcacttgt tgttcaactt gtatagaaac aatgctatca atgatttgta gagacatatt
     9841 tccccctcct ttcgtgattg cttgaacaaa ttttctaaga attatcttcg tattttttgc
     9901 tcaaaataaa agtattttgt tattaaccga ttaatcaaac aaaaaatgat taccagttca
     9961 tcgacataca cataatacct cgataaaaaa ggggtgtcaa cagctttgta aaatttattt
    10021 gcaacacccc ttttttgagg tatactaatt ttaggaggtg tggtcaatat gataaaacgt
    10081 ccaccaataa attatttaga acgaaaaaag atactcggaa caaaaataaa agctataaga
    10141 aagagtaaaa aattaacaca accagcattt ggtcttatga ttaacaacgg tcaattaatt
    10201 gacaaaaaaa ctatctacga atgggagaag ggaacctatt taccaatacc agagcgatta
    10261 tcccgaattg ctgatttggg aaatatgagt atagaggagt tagtttgtgg caatgtagaa
    10321 gaatatattt tgggaattat tctttataga gatagcattg tactagatgg tataacattt
    10381 ccagataaaa acctgttcca gcatcttcgt caacagttcc caccagtaca ctctaattta
    10441 gatacatggc tggatagata ttccaaatta gaaccagaga tgcaagaatt tattgctaac
    10501 aagacttgta ataaagttaa aaatgaaaaa attagtcttt tcaatatctt aaaaatagaa
    10561 gaattattca tcaatgctat tgtagaagaa tttgacaata acattctttt cctcactagc
    10621 agtatagaag aactactaga aagaatggta gatgaatggc tacctattca attgaaagac
    10681 atgtcttatc ccgaagaagc tgtgagagaa attactgata atataaacaa gctagaacaa
    10741 acgatttcat caattgggaa aaaatacact aagaaaaaaa tgaagggagg tgataccatc
    10801 tagtggtaag tactccttgt aaactaagag gagatgattt aatggcaaaa atatcaaagt
    10861 acaaaaagaa aaacggtcaa gtggcgtgga tgtataaagg acacattgcc accgatccac
    10921 gaactggtga aaaaataaat actacgagaa gaggttttag tagcaaacaa gaagctcaaa
    10981 aagattatga cgagtacaga catcaaatat tatacggtgt taaaaagaaa gcacctgata
    11041 tgacatttga agacctgtat aatgaatgga taactcatca gcgtaccagt gttaaagctt
    11101 caacaatcgc aatctcagta agatacgcaa ataatcaaat acttccagca tttggtaagc
    11161 ttaaactatc gaatatctct gtcccatatt gtcaaaaagt ggtagatgaa tggcacagta
    11221 aatatgaaag ttacgattat atgagaaaac agactgcaca aattttacgc tacggggttg
    11281 caatgcaata tattgacaat aatcctatgg aaaaaacact tcttccacga aaaaaagagt
    11341 atgagaaaaa tcgtaaattt tactcaaaag aggagcttaa taatttactg gatgctttta
    11401 aagattttgg taacatgaaa caatacgcct tcttcagatt attggcatac acaggtatga
    11461 gaaagagtga agtactcgct ttacagtgga aagacataga cacgtttaat aaagaactac
    11521 atgtcaataa aactcttgct gtagatgaat ttggtaaagt tataattcaa tctccgaaaa
    11581 caagagcttc tagacgagtt atctctttag atactgagac tctttctatc ctcaacaact
    11641 ggaaattaca gcaaaaagaa gagtatttaa aacttggata caatactagc tctaaagagc
    11701 aacatgtttt tacaacagtt aaaaacactc tatacattcc aaacactgtt aatgattggt
    11761 taagatatat tttgaaaaaa tataacttgc ctcgtattac acctcatggg tttagacata
    11821 cacatgctag tttattatta gaagcaggag aatctgtcaa ggtagttcaa caacgattag
    11881 ggcatgaaaa tagtaaagtg acattagaca tctatgcaca tattacaaat aatgcaccta
    11941 agaaaacagg tcaagacttt gctgatatga tggctcatca gtaaaagagc tggtcaaaat
    12001 ctggtcattt ctagtataca ggcaaaacaa aaaagctaga acccttatat atcaagggtt
    12061 ctagctccta taacaggttt acccaacact gccttccatc tcataactga tcaatcgatt
    12121 gagttcgact gcatattcca ttggaagttc tttggtgaat ggttcaacaa agcccatgac
    12181 gatcatttca gttgcttcag attcggtcag gcctcggctc atcagatagt agagttgttc
    12241 ttctgagatt tttgagactt tggcttcgtg ttctaaagag acttggctgt tgtggatctc
    12301 attgaatggg atcgtatcgg atttcgacca ttcgtccatg atgatcgtgt cacattcgat
    12361 atgagagatc gaacccgcac tgtttttggc aaaggttact tgtcctcgat agtttacttc
    12421 tccgccgtct tttgaaattg atttagagac gatagagcta gatgtatttg gtgcattgtg
    12481 gatcattttt gcacccgtat cttggatttg gtttgctcct gcaaaggcga tggagagcat
    12541 ggtgcctcgt gcaccttttc catctaagta aacacttggg tatttcatcg ttgtttttgc
    12601 acctaggttt ccgtcgatcc attcaacggt tgcgccttcg tatgcttttg ctcgtttggt
    12661 tactaagttg tacacattgt ctgaccagtt ttggatcgtt gtgtaacggc aatacgcatc
    12721 ttttcttgta aagatttcta cgatggcggc atgcaggctg ttacttgaat aagtaggtgc
    12781 cgtacaacct tcgacatagt ggatgcttgc tccttcgtca acaatgatca gcgtacgttc
    12841 gaattgcccc gtgttttccg cattgatccg gaagtacgtt tgtaatggaa catcgacttt
    12901 gacgcctttt ggcacgtaaa taaaggtccc acctgaccat acggctgaat tcagcgctgc
    12961 taatttgttg tcggttggcg gtacaagttt tgaaaagtac tctttgaata attcagggta
    13021 ttctttcaaa gcagagtcag tgtcagtaaa gacgattcct aacttttcga attcatcttt
    13081 catattatgg tacacgactt ctgattcata ttgtgcggaa gcaccggcta gataagctcg
    13141 ttcagcttct ggtataccaa tcttttcaaa tgtatctttg attttttctg gtacgtcttc
    13201 ccagtcacga gcgggtttgt cacttggttt ttggtaatat ttgatggcat cgaaatcgat
    13261 gtctgaaagg tcggggcccc atgtttgcat tggcattttg ttaaatgttt ctagagattt
    13321 cagacggaat tcaagcatcc attctggttc gcctttgatt cttgacatct cacggatcac
    13381 ttcttctgtc aagccttctc ctgtactgaa aaccggttcg acatcatcgt gaaacccgaa
    13441 tttatattct tctaattctg gtacgcccat gttctcactc ctcttccttg tggtaatgat
    13501 ggaaagctgc ggtaccatcg ttttccattg cttttcctag tgctttccag gcaagtgtcg
    13561 cacatttgat ccgagcggga aatttagcta caccaccaag catagcagca tcgcctaatt
    13621 ggtcgggatc tttgacttct tttccttgga caagctgtag aaagtctaca gttaaatgtt
    13681 ctgcttcttc tattgatttt ccgatgacgg cgtctgtcat catcgaggca cttgccgtac
    13741 tgatggagca gccactgccg tcaaaggcga tatttttgat gattccgtct tttacatcga
    13801 cttgcaattc gatcacatct ccacatgtgg ggttgttcaa ttcgatcttg cttgtcgagt
    13861 cagtcaagct tccacgatga tgcggatggg aagaatgatc caagatcact tgacggtata
    13921 aattgtctaa tttagatagt cccatgttgg aaaaactcct ttgttgccat gattgcttca
    13981 actaaacggt cggcgtcttg cgttgtgttg tagaaataaa agctcgcgcg agcagtcgct
    14041 gatacttcaa ggtacttcaa tagcggttgc gcacaatgat gacctgctct gacggctacc
    14101 ccttccatgt ctaaagctgt cgcgacatca tggggatgta acccatccaa attgaatgca
    14161 acgactcctg tccgaaaatt cgggtcttgt ggaccataaa cagtcaatcc atcaattgct
    14221 tgtaattttg gcaacacata agcggctagt tcttcttcat atcgatggat attgtccata
    14281 ccgagttttt gcaaataatc gattgcatgg cctaatgcga tagcgccggc gatatttggt
    14341 gtccctgctt cgaatttcca aggtagttct ttccatgtac tgtcgtataa ttcgacaaaa
    14401 tcgatcattt cgccaccaaa ttcaacaggt tccatttctt ctaaccattt tcgtttacca
    14461 tataaaacac caatacctgt tggtccgcac attttgtgtc cgctgaaagc aaagaaatca
    14521 cagtctagtg cttggacatc aatggccatg tgcggggcag attgagcacc atctactacg
    14581 atgaccccgc cgttttcatg gacaagttca gctaattctt gaacaggatt gaagacacct
    14641 aagacattgg atacatgagc aatggagacg attttggttt tgtccgtaat ctttttcgct
    14701 gcatcctcca tatccaggaa gccttcttct gttagttcga tatatttcaa gacagcacct
    14761 gttcgttttg ccaactgctg ccacgggata acattagaat gatgttccat gtaagagagc
    14821 actatctcgt ctcctgcttt gatatagcgg tcgccgaagg ttctggctac ccaattcaag
    14881 cttgtcgtcg taccacgagt aaacagcact tctgctgttt cttttgcatg gatgaacgcc
    14941 cgtacttttt ctcttgcttg ttcataatca tgggtcgcgc gttctgctaa cgtatggaca
    15001 ccgcgatgaa cattggcgtt atcgtgttcg taatagtacg ctaatgcctg gagtacttgt
    15061 tttggctttt gggtcgtcgc tgcattatcc agataaacta aaggttcatc attgacgtct
    15121 tgaaataaga taggaaaatc attgcgtagc tttttagcat cgatcatcca ttcaacttcc
    15181 cttcgattac ttcgactaat tctttccgaa cttgttctac agggattgct gttaagacag
    15241 aaccgaggaa tcctcgaatc actaagcgct ctgcttcttc tttatgcaat cctctactca
    15301 tcagatagta catttcttct ggatcaactc gtcctacact ggcagcatgt ccagcagtta
    15361 cttcgttttc atcgatcaaa agaataggat tcgcatcacc acgtgcttta tcagaaagca
    15421 tcaagacacg actttcttgt tgggcatctg caccttttgc ccctttcaag atgtggccga
    15481 taccattaaa ggtcaatgtt cctttttctc ggatcactcc gtgttgcaaa atatgcccga
    15541 tcgtatgagg ggctttattg gttactcgtg tatcgattcc ttggatttgt ctgcccgagc
    15601 tgattgccac gattttgact tctgcgtgtg caccttctcc tgctaaatct gagtcaaagt
    15661 cagcgatcac atgaccgtca ttcatgacac caattgccca gtcgacagaa gcatctcgta
    15721 aaatgtgccc acggcgattc atataagaag taatattttc tcctaattga tctacggctg
    15781 aatatttgat ttttgaacca gctttagcga tgacttccac gatgatatta cctgatgatt
    15841 ttgctacttg ttctttggtt gtctggaaac gttccagata agaaaattca ctatgttcat
    15901 ctgccacgat caacacatgc ttgaagaagt gctcatcgga tgcgccatct tgaatgaata
    15961 aggactcgag tggttcatcg atcacgacat ttttaggcac atacaggaaa ataccgctgt
    16021 tcataaaagc tacatggagt gcagtcaatt ggtcttcgtt catgtttact gcttttttca
    16081 tataatattc ttggactagt tctggatatt cgatcattgc agtgaacaag tcggtaaaaa
    16141 tgactccttt ttctgctaat tccacaggca gttgttcaaa aatcgtccat gaaccttgtt
    16201 ggacaagcac aggattgtct ttcattgcat caaatgccgg aatcgtgcct gtttcactga
    16261 tggtttgtgt ttctttcaca tcaaataacg accagcgatg gaatttcacc cgatcgatat
    16321 gcggcaatgg cagttcatct gctttcgcta gtgcgtcttg acggaaggtt ctcatccaat
    16381 caggttcgcc tttacttaaa gaaaaatcat ttaccgcgtc aagataatcg agccagtttt
    16441 tttctttcat tcttgctccc ccttacgctt cttcttcttt atagtcgatt cctaattctt
    16501 tactgatacc agcgtagcct tctgcttcca gacgtttagc taggtcagca ttccctgtca
    16561 tgactactcg gccttccatc atgatgtgaa cgacatcagg tgtgatatag ttcaataatc
    16621 gttgatagtg ggtgatgatc aatgcaccga aattgtctcc gcgcatttca tttactcctt
    16681 tagcaacgac tttcaaagca tcgatatcca gacctgaatc gatttcgtct aaaattgcaa
    16741 atgtaggttc taacatcaac aattgcagga tctcattccg tttcttttct ccgccagaaa
    16801 aaccttcgtt cagataacgt tcagccattt cttctggcat atttagtaaa gccatttttt
    16861 catctaactt tttcaaaaat gccataacag aaattttgtc gttttcatca cgtttcgcat
    16921 tgatcgctgc acgcatgaat tccgcattcg tgatacctgg tatctcactt ggatattgca
    16981 tagctaaaaa taagccaagt cgcgcacgtt catctacttc caagtctaaa acatttttcc
    17041 cgtcaaacaa aatttcgcct tctgttactt catagtttgg attgcccatg atagcggcag
    17101 ataaggtaga ttttcctgtg ccgttaggtc ccatgatggc atggatttct ccggttttca
    17161 ttgttaggtt tacgccttta aggatttctt tttcctcgat cgacacatgt agatttttta
    17221 tttctaagac agacatgcat tataccccca aattctcttt cgttctcact cattttagac
    17281 taattgagaa tgaagcgcaa tgaccttcct tactaaaatc aaatatcttt tatttattat
    17341 taaacaattt tgatttctaa attagaatag gtataaaaaa gatgagaaat aagccattac
    17401 agacttattt ctcatctttt ttgttccata ccttattttg catcagcctt tactggttta
    17461 acagagccgc cccatttgtc tgtgatccat tcttggactt ctttgcggtg aagcacttca
    17521 atcagttttt tgattttgtc actgtcttca tcacctttac gagtcgcaat aatattcacg
    17581 taaggagagt tgtccttttc aagcaatacg gcatcttcta atggattcaa ccctgcgccg
    17641 taagcgaagt ttgcgttgat tgcaactagg tcaccttcat cattttggta cgtagaagct
    17701 aaaagtgatg ggtcgatcgt atggttgaac ttcaagttct tagggttttc ttcaatatca
    17761 tcgaatgttg ctgtttccag atctactcca tctttgactt tgactaagcc agcatcttgt
    17821 agaattgtga tgatgcgtcc ccagtctgat tcagaatttg aagtaatgat tgttgcacca
    17881 tcttttaatt cggaaacatc tttgactttt ttagagtaca agcccattgg ttcgatgtgg
    17941 acagcaccgg cattgacaaa gtcatatcct ttttctttga tttctttgtt caaataagga
    18001 atatgctgga agtaattggc atcaatgtcg ccttcttcca aagattggtt cggtaataca
    18061 tagtcatcga atttgacgat ttcaagatcg attccttcat ctttcaataa tggtttcaca
    18121 tgttccaaaa tctctgcgtg cggtgtcggt gaagcgccga ctttcaatgt cgtgtcgtct
    18181 gctttatttg ctgaatcttt gctgtcgctg ttgttaccgc atgctgctaa tcctacagtc
    18241 agcaaaagcg ctgctgcaaa tccaaaaatt ttctttttca tatcctacat ccccctatat
    18301 cctattatct tttgtctgtt ttcttagtta agaaatcacc gattccttga acgataaata
    18361 cgattagtaa aataattaaa gtcgcaacga atgtcactgt tagattacca cgctggtagc
    18421 cttcctgcca agcaagacct ccaagtccac ctgcgccgat cgcgccggcc atcgcagtaa
    18481 atccgatcat cgagatcgtt gtaactgtta atccagaaat caatgcggga atactttcag
    18541 gtatcaagac cttccagatg atttcccagt aacttgctcc cattgcatct gctgcttcca
    18601 gtacaccagg gctgacttca cgaaaagcaa tctctactag acgggcataa aatggtgctg
    18661 cggataaaac aagagctgga atggctgctt gcgcacctag catagtgcct acgattgctt
    18721 tagtaaatgg aatgatcaaa accatcagga tcataaatgg cgttgaccgg aaaatattac
    18781 tgatgatcga tacgattcca taaatgattc gagcatacgg tttgtttttg cgtccaatag
    18841 agtaaaggat caatcccaaa actaatccga tgatcgtcac taagaccatt gagattaatg
    18901 tcatatatag tgtatcaagg atagcctgat tcatcgtttc cgggttgatt tgtttaaaat
    18961 ctagatatgt ttctgaaaag cttttatcca cgatggatca cctcaattcc tactccttgt
    19021 tctttgatga acacttctgc ttgatctaat tctttggttt caccagtcat caagacagtc
    19081 aatgaaccaa aggaaccttc tttcacttgt ttgatctttc catacacaac gttgacatcg
    19141 atgttatagc gtcgaatcgc ttgggaaatg actggctcgt tggcatttct ttcgctaaag
    19201 gtcaaagttg ccaaacggcc gcttggattt tctttgatga gttcagctaa tagttctgtc
    19261 gaatcagctt ctggttcgat gtcttgttgt acaaagcgtt tggttacttc ttttttcggt
    19321 tgtctgaaga cagtcaatac gtctccttct tctacgactt tccccaactc catcacagcg
    19381 actttgttgc agattttgcg gatcacattc atttcatgtg tgatcaagac gatggtcaaa
    19441 ttcaatttct tattgatcgc taacaataag tctaatactt cttctgttgt ttgcgggtcc
    19501 aatgcgctag tagcctcatc acaaagcagg atctcaggat catttgccaa tgctcgtgcg
    19561 ataccgacgc gttgtttctg tccgccggaa agttgtgaag gatatgcttg tccgcgtcct
    19621 tctaacccga ctaaacgcaa cagttcttct gcccgttctt tacgtttgct ctttggtacg
    19681 ccagctaatt ccagcggcag ctggatattt tcaagtacag ttcttgacca taacaggttg
    19741 aaatgctgga agatcatacc gattttcttt cggaatgtac gtaattcttt gttttttaat
    19801 tttgtaatat cttgatcgtt tacgatcact tcgccgctcg tgggaagctc aagtccattc
    19861 aggagacgga ctaatgtact ttttcctgcg ccagaatagc cgacgattcc ataaatatcc
    19921 cctttttcaa cgtttagtga aacgtcattg acggcttgga tgtttccgtt tttcccattg
    19981 aatgtctttt tgacattcgt tagttggatt aatgacatgc cattcacatc tttctcttat
    20041 gatttttcaa acaaaaaagc cttcgccctc ttgccttaag caaaaggacg aaagcttgaa
    20101 cttccgtgtt accaccttga tttaccgtta cttcgcaata acagcctctt ccagtacttg
    20161 gacttagctt ctttccgtat actgtcgcgc tgtaacgggc actcccgtag taagtttgct
    20221 tacgctcgct tactctgctc taagaccatc ttccatctca ttttcgttac ccattttcag
    20281 caaacagggc tctcttcaaa cgtccgtgac atgtactctt cttttcaaag ctttctcgtt
    20341 tcatatagga ttgcttatga ttatagcaac tggaagaatc agtgtcaact tttttcaagt
    20401 gattttctga ttttataatt cgtcaaattc ttttggatca acatctttga aacttaagat
    20461 ccacgcattc atttcatctg aatcattcaa gatattgata tcttcaccga ttttatcatt
    20521 aacagaagaa actgtacccg ttagcgggct ggcaaattca ttgaccgctt tttccgcttc
    20581 aacttcgatc agaggttcat ctttttttag cgattgtcct gcttttggca gattggcaaa
    20641 cgtgatgtct ccaagttctt cttgcgcttc atttgtcaaa ccaacacagt attcttttcc
    20701 attaaataag atccaaaggt tgtccttttt cttcaaatgc ttgcctgcca ttttatatca
    20761 gttctccttc ataaattttt tcattgaatc caattgcaac aagctggtcc tctcgtaaga
    20821 tcagtggtcg cttgatcagc ataccatctg tcgataagac ttggctcgct tttgaagccg
    20881 taaactcgcc tacttgatct ttcagtccaa gttcccgata tttcatcccg cttgtgttga
    20941 acaggcgacg gattggtact tgactcgttt ctagccaagt ctcaatcgtt gaggccttcg
    21001 gtacttcttt gatcatatca acggtttgat aactaacgcc atgctggtct aaccacgctt
    21061 tggctttttt gcatgttgaa catttcgggt accagtaaaa ggtgtacaat tttcttacct
    21121 ccttgaagat cttgcaggtg cttcattttg gtatctcatc gataatatca acccgatacc
    21181 gatcatgttc cctaaaatcg aggatcctcc ctgactaatg aaaggcaatg ggatccctgt
    21241 taaaggaagt aatccgatat ttgctccaat attctcaaag acatggaaca gcagcatcat
    21301 gatcaaaccg cttgcgatat aggcgtaaaa ttcattattc gtatcgaaac acacccgaat
    21361 catacgatag attaaaataa aatacaagaa aatgacgaat gcaccaccga taaagccgaa
    21421 gttttcaccg ataacggtaa aaatcatatc ggactcacga acaggcacat agatattgct
    21481 tacgttgaat cctttaccaa acaagccacc ggtaccaatt gccaaaagtc cttgggccgg
    21541 ctgataactc attcctgacg tatcatggaa agggtccagc catgaatcaa tacgtgcaaa
    21601 ctgataagat ttgaatccaa ctttataaag caagtctctt ccgccttcag tcgtaaccaa
    21661 gaagatcgtt cctgctccaa cgactactgc caagattact acaggtgcga tgatgcgcca
    21721 agagattcca gacatcagga aaatcccgcc aaagatcgca agaaagacaa gcatcgttcc
    21781 aaagtcatct tgggccatga tcaatgctaa aaccggtatt gtcacgatca agagcttccc
    21841 aatcagccaa aagtccgatt tcatcgtccg ttcttttact tgcgtattat gtttagttac
    21901 gatcagtgcc atcatcaaga tgtacgcaat cttcatcaac tctgctggct ggaaagtaaa
    21961 cgcaccgaat cgaaaccagt ttttagaccc tgttgaagca gctaaattct gatcatagaa
    22021 tttgagcaag agtcccatta ccactaaccc caaaccataa aggtaaggag tcagtttcca
    22081 gatccatttg gaattcaaat gcatgatgac aacgattgct gccgctccaa ctaaatacca
    22141 gacaacttgc atccctacgc ctttcaagac tgctggattg ttcggatcat ggctaagtgc
    22201 tacatataat gagagcagac caatcaaaca taacagaaag actggtaaaa tcacgccata
    22261 atcaatacga ttatcgttcg aagcttttaa ttttttttcc atgtttcatc ccttttctta
    22321 aaaaccatac tgctgattat tatagcggtc cagttagata atggaaagtc tttccataga
    22381 catttgggaa aaattaactt tccgtttgtg cagttgcttg agcaaatgca gtacaaaagc
    22441 ctactgctta tctttagtgt atcatagaac tttttttgat acatgcaatt atctaccagc
    22501 tgagattctc ctcgctgtga gaagtttctg tgctttcatt gtatcgaagg atctcttcgg
    22561 caatctctag cagctgatca gaaaccagat tggttcgttg gatgaaataa aaatatcgat
    22621 ggaaattcaa cggagtcatt ggcacatttt ctggaacaga gcgcttagac aaaatcgagc
    22681 gaccgattcc ttccttcagc aacgcaacaa ttacatcgtt acttttcacg atcatcttct
    22741 tatcttggat attcatctct tcaaaataac gctgcgtgta atgaaaaaca ccagagtttg
    22801 cttctcttac aagccacaat ttgctttttg ggtccccagc gatgactagc tggtcttcca
    22861 tcaaagagta tctttgtacg ccggaagtct ctaatggctt ttcgataaaa ccaaaatcgg
    22921 attcatgttt cgctacttgt tctaatgatt catgagaatt cgtgatttcg atctcaaaat
    22981 gcactttagg aaaccttttg atcagatgtt tcatcaactg aggcaaaaga tagacagcaa
    23041 aggtgtggga agcaacgatc cgacatactt cttcttcatt ttgttcattg agcaattttt
    23101 gctgagtttc ttcccattct tctaacaaaa ccagtgtccg ttcatataat aagtcggctt
    23161 gcttagttgg aagcatctct tttcttccgt tacgttgaaa aagatcgata ttcaattccc
    23221 gctccaactg tttgatttga ttcgagactg ctggttgaga taaaaacagt acttctgctg
    23281 ctctggaaaa atttcttgtt tcatatactg ctcgaaaagt cgttaataac ttaaacatgg
    23341 caccacttcc ataacttttt atgataatta ctataaaaac tatttgattt aaatatacaa
    23401 aaaaccatct ataatagaaa tgtacaacaa aacagttgct gatccaacta gtaacttaga
    23461 tgaagacaat cagctgtctg cttgctcgta atcatacatt cctattcatt ttacaatgta
    23521 ttcgttcatc ttggtactaa aatctattaa ataaaaagga gaatattata tatgaagaac
    23581 tttttgaaaa ttattccgat cccgatctgc gggttgatcc ttgggttagc ttcccttgga
    23641 aatctgttta aagactatca ccatgttgct ttaggcaatc taactggcgg tatctctatg
    23701 attcttatga tattcatttt tgttaaattg tttatcttat tcgaacacac gaaacaaacg
    23761 ttgcatgatc caatcatcgc ctccgtttca ccaactttta cgatgtcact catggttatt
    23821 tgtacttatt ttgtttcata tgaaagtatt gcaccaattg tcaaattcat ttggctgtgc
    23881 gctgttatct tccaagtgat ccttgtcttt tatttcaatt atcaccatgt tatcaaagct
    23941 gatctttcaa ttgaagcaat ttatccaagc tggtttatca tttacgttgg atttggggtc
    24001 atcactgtaa ctgccgggaa cttcttccca cttattggaa aaattttctt ttggatttca
    24061 ttgatctgct atgcgttctt attgccgatc atcatcaaac gtgtcttttt cgtaagaaat
    24121 atggctcatc cgacacgtcc gctgattacg atcgttgcag cacctggttc tctttgccta
    24181 acaggttatc ttaaaaactt tgctgaacct aatttttatc ttgtcgtagc attgttcgct
    24241 ttatctcaag tattgtattt cgttgtgctg tctatgcttc cacgtttgtt caagcttcgt
    24301 ttttatccaa gctatgcagc ctttacattc ccgcttgtta tctctgcaac agctatattt
    24361 actactgttc attacttcaa cagtttaggt atctatagta gcgtattgga tttcttaaaa
    24421 gttgcggaat gtctgtttgc aagtgtagtt gtcttctacg ttcttgctca ttacgtacgt
    24481 ttcttaatta aagaacatag caaacatgga aaatttgttt ctaaaaaaat cgcttaataa
    24541 tcgattcaat aggttgtgaa aaagctgttc tgcatcaagt aataagaacg gccaaacaaa
    24601 aacagctgtt catgtttttg tactggttgg gcttattacc gcagatgcaa gcttttgaac
    24661 accgtttacc aagaggtcgt aaaagaagtg gtcagcttca agaaataaga aggaatttac
    24721 gaaaattgct tctcaaattt ttgtgaaatt tcggcttatt tccgtagaag ctacttctgg
    24781 aacaccgttt attcgaaaca agaggtcgta caagactttt gtcacgacct cttgttcttt
    24841 tgtcaaaata ctggtttagt gctactatgt tcgagtatgc ttttagtatt tattgacgaa
    24901 aggaaccgat caaatgaaat cattgtcaca tccaataaaa tctgtaaagg attcagcggt
    24961 ccgcgaaatc ttacccggtt tactgctttc ttttgccatc gctgccttca gcaggcttct
    25021 tgctatttat ctgccacagt taggtggagc gactattgct attttattgg gtattttatt
    25081 aggaaatact ttttttaaac agcctgttct agcaatcggc acaaaatttg ctgaaagcaa
    25141 actattagaa ttctctgttt ttttactagg tgcaactgtc acagctcaaa cgattgctca
    25201 aattggtgta aaaggaattg tttttgtgtt actgcaaatg tcagtcacta tcatcggtgc
    25261 ttacttgatt ggtaaaaaac tgcaattcag cgatacttct tctttgatga tggctggagg
    25321 aaacgctgtt tgtggttctt ctgctattgc ttcaatcgcc ccttccatcc atgcgaaaga
    25381 ggaagaaaaa ggacagatca tcacactagt caatctactg ggtactgtga tgatgttgac
    25441 tctccctttt cttggattac agttgtttgg tggaaatctc attgaaaaaa gtgcactttt
    25501 gggtggtaca ctccaatctg ttggtcaagt agtcgctggg gcaagcttac ttgacaattc
    25561 tgttgtcgaa ttcgccatgc tatttaagat tctacgtatt atgatgctcg ttgttgttgt
    25621 ctatctgttt gaaaaattca tgcacaagaa acaaccagct ctgtttgaaa cgccaactga
    25681 tacgcagaaa aaacgaaaat taccgcttcc ttggtatgta ttagggtttg tgatcttttg
    25741 tttggtcaac agcttgtttt ctttacctgt agttattgac caatcggcac attttatcag
    25801 cagctggttt gagatcactg ctttagcggc aattggtctg cgtttagact ttgcaaaatt
    25861 catgaaagaa ggtacacgct tcttatctta tggattagct gtcgggcttc tccaaacagt
    25921 gatggcttta ctatttattt ggctgatcaa agtttagaat gaacatgaaa aaacagttgt
    25981 gattctcgca tggaacaatg tccgaatcac aactgttttt aattttgcag atatccttct
    26041 aacaacaaca attggcgttt gatttcaacc ccatctattg cgtttcccat atatcctgtc
    26101 agttttccat tttgcccgat cactcgatga caaggaataa cgattggcaa tggattggtt
    26161 cgatttgcct gcccaatcgc tcgcacagct ttcggactat caacagcaac ggctacttct
    26221 ttatacgttc ggatttctcc ataaggaatg gtcgacaaag ctttccagac attttgctga
    26281 aaaggcgtac ctctctttat agataaagga acatcaaatt cttttctttt tcccgcaaaa
    26341 tattcttcaa actgttcttg tgtgcgcgtt aaaattcgct gattcgctga ttcgctttct
    26401 gaaattggct gaaaagatac agcagtcaat ccggattcat ctgcatctaa ccataaaaag
    26461 ccgataggtg tgtgtatttt cattttgttt gctcctagtc aatagactgg aacttcctcc
    26521 tttttctcta tccaaaacta cttgccaact aataaaaaat gttcttaaaa gtttgataaa
    26581 ccgaggaaac atggtaaact aaatacgtta gtttacaaaa agaatacctg ttcttttacg
    26641 taaaatcaaa ttttaaaggt gttatttaag gtttattaaa ttttgaatgt aaaccatata
    26701 taaacatacg attgaaagga tagaaacgat gaaagtatca attgttgtcc catgttttaa
    26761 tgaagaaaaa actgttcctc ttttttttga tgaggttgaa aaattcaaag gtgcccatga
    26821 gttcgaatat ttgtttattg acgacggatc aaaagacggt acgttagcag caatcaagaa
    26881 gctagcctca gcttatcctt atgtccgctt tgtctcattt tccagaaatt ttgggaaaga
    26941 agcggctctg tacgctggat tgcaagcttc tacaggagat ttagtgaccg tgatggatgt
    27001 tgatctgcaa gatccgcccg aactgcttcc tgagatgatc aaccggatca ctacttctga
    27061 tatcgattgt gtcggcaccc ggcgttcgac tcgtgaaggc gagcctgcga tacgcagttt
    27121 ctttgcgaaa aaattctacc agttgatcaa taaaatcagc gatacagaaa tggtcgatgg
    27181 cgctcgtgat ttccgcttga tgactcgtca aatggtggat gccgtcctag aattaacaga
    27241 atacaaccga ttttcaaaag gattatttag ctgggtcgga tttaaaacag aatatatttc
    27301 ttatgaaaac cgcgaacgtg tggctggcga aacatcttgg tccttctgga aattattcaa
    27361 ctactcgatt gatggtatcg tgaacttctc ggatgcaccg ttaacgattg cttcatttat
    27421 cggcgctttg tcttgtgtcg gttctggtat tgcaattttg ttcattattt tgcgggcatt
    27481 gttgtttggt gatcctacat caggatggcc ttccatggtc tcgatcttcc tgttcattgg
    27541 tggaatccaa ttgttatgtc ttggaatcat cgggaaatat attgggaaaa tcttcttaga
    27601 aacgaaacag cgtcctgttt atatcataag agaaacagaa aaagtgaagc caaatgaatt
    27661 gaagcctcac caataatata cgtcaaatga agtaaacatt tttttaaatg aaaaaggact
    27721 gtgacaaaag tcttgtcaca gtcctttttt ccgaataaat ggtgttccag aagtagcttc
    27781 ttcggaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt
    27841 atttcttgaa gctgaccact tctatcacaa cctctttcat atcttattac ttcttcattg
    27901 ctgcttttag tgctgctgct aatgggcttt cttctggttc ttctgcttgt gcatattttt
    27961 tcagcaagcg tcgttcttca tgtttggtca ttttcttctt ctgctctttt ttatcaagca
    28021 atttttctgt cgttccatcg tatttacaac ggaaataagc accgttttta ccttcgatga
    28081 tttccatttt ccggtgacat tgcgggcatc gatgattaga gacttttgga tcttttcttc
    28141 tgcggtagtt gcaatctgaa ttggtgcaga catagatttt gccgtcacgt gtgtttttct
    28201 cgcgcagatt ggccccacat tctgggcatt tcttttgggt aatcgagaaa tcttgatatt
    28261 cttgtttgct tgatttgatc tcattcacca agcgttttgt ttctttttcg attccattca
    28321 agaaggttcc tgcttgtttc tttcctgcag caatagcttc aaggtctttt tcccagcttt
    28381 ctgtcaaatc tggtgttact aaagagggat ttaccaatac taataattgc ttacctttag
    28441 gagatacttg cagtccttga ggggtacgtt ccatcagttc agatttgatt agtttctcga
    28501 taatctctgc acgcgtagct ggggttccca gactgtattt ttccatttga ccaagcaagc
    28561 ttgcttctga caatggttta ggcggtgcag atagttcttt tttgatggcg aaatcgggat
    28621 tgattctcat tcctttttcc catttgactg tttccgtctc agtttcagtt tcgcctttcc
    28681 atcctgcttg gacgacacga ttttgccgaa aaatgaatcg ttctttacca aattcaaccg
    28741 taacttttgt ttggctgact ttatggggtt gagcaaacag tcctaaaaaa cgagagacaa
    28801 tcatttgata gatttttcgc tcttcattac ttaatttttc ataacgtgct ctgttttcag
    28861 ttggcaaaag tgcatggtga tcggtcactt tactgtcttg gaatactttt gtctgttgga
    28921 cgatggctcc gtttttcaaa tactgcttga cctcaggagc aaagtcgctg actgcttgca
    28981 atcgttcttt catcgtactt ttcatgtcat ttgtcagata tttgctgtct gtacgcggat
    29041 aagtaacgat cttatgggtc tcatacaaac tttgtacaag acttaatgtt ttctttgcag
    29101 aatagccata gcgttgattg gcttctcttt ggatctctgt taaatcataa ggcaatggcg
    29161 cagattccgt ttttaatttt tcttggactt ctgtgacagt tcctttgctt gtactgatca
    29221 attgaacaag tttttgtgct tcttcttgtg tttttaaagc aaattgattt ttttgaagca
    29281 ttttggcttg ttcttttcca gcttgcaacg tgatcgtgaa atacgtttgt ggacggaact
    29341 gctcgatttt cttttcttgg tctctcacta atgctagtgt cggtgtctgc acacggcctg
    29401 ctgataaatt atccgcgtac ttcacagtta aggctctggt cacattcaat cctaccagcc
    29461 agtccgcttt tgcccgggca agagcagatt catacaatgc atcatattct ttcgcaggtc
    29521 ttagttgctt gaatccagtt ttgattgctt tatctgtttg agaagagatc cataaccgct
    29581 tcactggttt attgaaatgc acatactcta agatccagcg agcaacaagt tccccttctc
    29641 ttccggcatc tgtcgcaatg actgcttctg agacatcttt tcgctgagcc agctgcttga
    29701 ttgcttttaa ttgatgtcct gttttaggaa gtggtttgat tcctagtttt ttagggatca
    29761 ttggcaacgt gtccatttgc cagctctgcc attccttatt cagatcttcc ggcattttca
    29821 atcctaataa atgaccaagg gcccaagtaa cgatcacctt gggtccttca taataatttt
    29881 tgtgcttttg attagcacca agcaccttgc ttaggtcttt cgcaacgctt ggtttttccg
    29941 ctagaattag ttgtttcata aaattccttt ctgctttcta ctgtgatggg ctgatgattt
    30001 ttcaacaacg caagtccctt gtattgctgt aaatcatcgt cacactcttc acaccagcgc
    30061 tcatctccgt cattccaaaa ggcgctgaga aagttacttt ttgcattgat atattcataa
    30121 ttttgatgga tttcgttcca ttgtttttcg tacgcttgtc gtgcttcttc gaaagactcg
    30181 aatgtttctt cttcttcaat atcttcttcc cagtcttcaa aaaaccacca aggctcattg
    30241 tccccgaaca tggtaattac ttgatacata ctcgtgctcc ttctttcagc ttttctagac
    30301 tcatcttagt cacttttcaa gaaagttgca agaattttct cttataaaag ggtcatttaa
    30361 tgaacatctt aaaattgtta caaatgaatc tgattgttta aggcaaagta gcgataaagt
    30421 tattgatcat ccaaaaatag ctgtcttctg gatcgattgt tggttctttt gtaaagtatc
    30481 ctaaaaaacg taaggaaaga taaccgtgga tcatactacg aagttccctg cttcgatgaa
    30541 ctttttcttc tttcgttaat tgaaaagcct caaatatctg caaaatgatc tgattcgttt
    30601 catggattcc atctaataaa acagcttcat tggtatgcgg tacactgatc agtaactcat
    30661 acactgcttg atttgaaaaa gcaaaggatc gataagtttc tgcatagatc tgcagtgctt
    30721 cctgaccact ttttcccaca agagcccgtc gcagctggtc attaagttcc tgcagaagtt
    30781 tcgctgttag cgctgttttg acatcacgaa tatttttgaa atggttatac agcgagggag
    30841 ccttgatatc caagcgtttg gctaaatgct gaaaggttat ttgctgaaca tccatctctt
    30901 ctgctagttc tcgaaagcag tcgatgattt tctcttgaga cagatttctt tttttcattt
    30961 ccatgtttcc tctcataaaa taaatccttc actctttcag tttaccacga aaaagaaaga
    31021 gaaaaaagag actttcattg ataaactaca tattgtagtt tataataaag agctgtagat
    31081 gaaaattttc agaaagaagg agtctaattg gctgcaagaa taaaggaaaa attatttagg
    31141 aaaagagaaa cttgggaaca aaacttgttt gtcttatggt ttggaacatt tatggctggt
    31201 atcggattta gtttagtcat gccttttatg tcactttaca tagatacgtt aggtaattat
    31261 agtacgtcac aattgaactt ttggagcgga ttgacttttt catctacttt cttggtgact
    31321 acgcttatct ctccttggtg gggaaaatta gccgaccaaa aaggaagaaa attgatgctg
    31381 ttgcgtgctt cattaggaat ggcaatcgtc atttctttga tgggttgcgt gactagtgtg
    31441 tatcaattag tcgcattgcg tctgcttcaa gggatttttt ctggttatat cagtaatgcc
    31501 actgcccttg tcgcaactgg gacgccaaaa gaaaaaagtg gtcaagttct tggaacctta
    31561 gcaactggtt ctgttacagg aacattactt ggaccgcttc ttggcgggat ctctgcttca
    31621 gcgtttggct atcgccctac ctttttcatt acagggatca tcttattttt cgtcttcttg
    31681 ttgagtctgt ttttcgtcca tgaaaaattt gtcccagtga aaaaagaaaa catggtttct
    31741 gccaaacaga ttttcaaaga cttgaaatat ccacacgtcg tcatcggtat gttcgtcaca
    31801 acgatgatca tccaagcatc caacaattcc attagtccaa tcatcagtct gtacattcgc
    31861 caattgctgc atggacatgg gaatgttacg ttgatcagcg gaatcattgc ttcgatacct
    31921 gggattgcca ctttgatagc cgctccaaga tttggtcgct taggtgacaa gattgggagt
    31981 gaaagaatac ttgcgattgg tttgggtttt gcgattctgg tttatatccc aatggcattc
    32041 gtcacgaatg tttggcaatt agctgcctta cgttttttga ttggtatctc tgatgcttgt
    32101 ctgctccctg ctgtccaagc attgatcaca aaatattcgc ctcatcaagc tgctggacgg
    32161 atcttcagtt ataaccaatc tttccaagca actggtaacg tagtagggcc tatgatcggc
    32221 tccagtgttt ccagtgcatt tggttatcgt ggcgtctttc tttcgacttc tcttcttgtt
    32281 ttggtcaact tcttgcttgt acgacacaat acacaagaga tccatcagca agaaactgca
    32341 gaagaaaaag cttcaggagt ctccatagct tcagggcact ctccttctca tagacaataa
    32401 gcgtaaatga aaaacactgc ttgaacagtt gtcacagact gtccttgcag tgtttttgtt
    32461 taggcctgtt ttttcaagaa ctcagataag aggaataatc ccttttccaa ggtttctttt
    32521 ttacaacagt aacctagtcg ggcatgcttc ggtaaatcaa aggcacttcc tggtactaat
    32581 agtactcctg tttcttctaa taaattgata caaaatgtct ggtcatcttc ttcgatggct
    32641 aatttaatga aagaagtaga gacgtaattg ggagcgacaa gttcaacttt cggttcattg
    32701 gctacccatt gttttaaaat cacaaggttc tctgtaatga tctttttgtt tcgcgccaat
    32761 attttttctt tattcttcaa tgcatgtaca gcaagatcat ctgacaggac tccgccgcaa
    32821 atcatcgtgt agtctcgata ctttcgaaaa acttctgcca actcaggtcc agtagctgtc
    32881 cagccaatac gaatacctgg aatggagtag gttttggata gggaattggt tgctatacct
    32941 ttttcataga catctacgat cgacaagaat tcacctttat ccgttaaagg cgcatatact
    33001 tcatctatca atacatatgc atctactgta cgagcaattt ctgcgatttt ttctaaagtt
    33061 ttccgatcta gcaatgtgcc tgtaggattg tttgcactat tcaaacagat gatttttgtt
    33121 tcaggtttga ctaaggcttc tagctgttct aaatcaaact gccaatcttc ttcttccttc
    33181 aaatgaacaa agtcaacagc ggcacctaaa gatttgggga tgtcatataa ttgctgataa
    33241 gtaggaagca tcgaaacaac atggtctcct gcttccacta acgcgtatag agctaaaaga
    33301 ttagcaccag tcgctccatt cgtttgtaag atgttttccg gatcgacggt ttgataaaga
    33361 gaagcgactt cttgtttgaa aacatctgat ccttcgatcc agccatagtc caatggttcg
    33421 tttttgcgtt tttcgaaaaa tttacttacg ctagtcccat ctaagccaac gagttcgtct
    33481 aatgtcaatg cttcgatcga gctttgtgca atatcatata ccgctttctt ttcccattta
    33541 tttagccatt cttctacacc gaaatttgcg attttcatgt ttctttcccc cttatatctt
    33601 gatccagcct acagcaagca acccgattga aagcagtgcg gccacacaaa tcagcccaat
    33661 cattactttt tcttttcccg taatttgatg tttggtttca cgacaagcga tccagtagaa
    33721 gcaaaaacca ggaagatagg caatactaca taaaaggaca taagaaaatc ctgctaaaat
    33781 gatgcccatc aattcaaaaa ctacagcaat cccaccgatc actacttgtt ttttgattcc
    33841 ttcttttaaa gaaaactgga cttgatagat cccgacaagt agataacaaa tcaaaattgc
    33901 cgctgtacat aaagaatagg caaattgata ggcataatca gtaactaaga aagtcaataa
    33961 gaatagatta gtcaagattg cagtaacaac taacgaataa gttggggcat tttttttatt
    34021 cgtttttccc caacctttcg gcaacagacc gtctcgagcc atcaacatcg ttgtttctgc
    34081 tgggagcatc gtccaagaca accaagaacc aatgatcgaa ataatcaagc cgatattgat
    34141 cagcgctgct ccccaatttc ccacgattcc ttttaaaata tttgccatag caggttgtga
    34201 aatagttgct aactcttctt gagataagac gccgtatggc aagatcgaag caaggatata
    34261 gataaacaat aaacaaagca gaccaatgat cgatgcttgc tgggcttctg aacgtttctt
    34321 cgcgcggttg gctaaaacac ttgcgccttc gatccctaca aagacccaca tcataaccat
    34381 caaacaacct ttaatctgca accagatcgt tccttgctgc cctgttccat tcactagatt
    34441 atcagatacg ttaccccaaa aatcagcagt gaatatattc actttaaatg cgataaatac
    34501 aaatattaaa aataggaata atggcactaa tttacagatc gtcacgattg tattgatgac
    34561 gctcgctcct tcaactccat tgtttactaa taaagtcaga ccccaaataa aaatacttgc
    34621 aaataaaata gaaggaacat tttgtccacc tttgaatatc gggaaaaatt ctcccatggc
    34681 gctcatcagc atcgttgcaa aagcaacatt tcccagccac gcagacaacc agtaacccca
    34741 accactgatg aagccaccta atttaccaaa tgaagcttcc gcgtaaccga aaataccacc
    34801 atctagatct ggacgctttt ctcctaaatg attcagcgat aataccaaag ctaaaatacc
    34861 aatcccaacg attatccatg aaaggatcgc tggccctgga gctgcagaac tagccagatc
    34921 acttgtgatc ccaaacacac cgcttccgat tgacgaactg acaaccagag caaccagtcc
    34981 ccatttgcta atacccgtcg atttcttttc catttccatc accctcactt ataaaattga
    35041 acaaatcact tgcaaaagat tcccttctat tttgcaactg ctcttgctta acaaaaagca
    35101 actttattca tattattgga tattatgaac tttttacaca agtatttcaa gtataaaatg
    35161 aataaaaatt aattttattg caaaaaaaca gctatgctat ccaaaagaaa aggtgtttct
    35221 tttgttagcc atagctgttt tgattcattt aatatcgctt aaacgtattg taaaactttt
    35281 gtttcttgat atgcgtggtc gatcaaacca ccacccagac attccattcc atcataaaat
    35341 acaactgctt gtccaggagt gattgcacgt acaggttcgt cgaatacgac ttctgcacga
    35401 ttatcatcca ataagcgaac tgtcacagga acgtcttgct gacgataacg gaatttcgct
    35461 gtacacttga attcttttgg cattggttca ttcgtcgtaa agtggatttc acttgcatcc
    35521 aagcttgtgg catacaatgc aggatgatgg aacccttgcc ccacatataa tgtgtttgtc
    35581 gctagatctt ttcctacaac aaaccagggt tcttgcgaat ctcctccgcc accgatccct
    35641 agtccttgac gttgaccaat cgtataatac atcaagccag catgttgtcc tttgacttca
    35701 ccatcaagag tgaccatatt tccttttttt gctggcagat agttgctcaa aaattgtttg
    35761 aagttctttt caccaataaa gcagattccc gttgaatctt ttttcttagc agtcgcaagg
    35821 cctgcgcgtt cagcaattgc ccgtacttct gatttttcca tgccgcctag agggaacatc
    35881 gtttttgaaa gctgttcttg tgataattgg ctaaggaagt aagtttgatc tttattattg
    35941 tctactccac gaagcatgtg gaccacgccg ttttcatcgc gagtgacttg cgcataatgt
    36001 ccagtagcta cataatcggc gccaagatcc attgcataat ccaagaatgc tttgaatttg
    36061 atttctttgt tgcacatcac gtctgggtta ggtgtacgtc ctgcgcggta ttcggctagg
    36121 aaatattcaa atacacgatc ccaatattct ttttcaaaat tcacagagta ataaggaatc
    36181 ccgatttgag cagctacttt cgctacatcc ttataatctt ccgtggccgt gcatacaccg
    36241 ttttcgtccg tgtcatccca atttttcatg aatattccga tcacgtcgta tccttgttcc
    36301 tttaacaaaa gtgctgttac agatgagtcg acaccgccgc tcatccctac gacaacgcgt
    36361 gttttgctgt tgtctgtcat gatatcacca tcatttcaat tagattctga aacatctacg
    36421 agttgaaggt cgcaactttt cagtatttta cattatacgt catatttctg aaaatttcat
    36481 cccttaatta tcaagtcgta aaaatactgt tctactggac ctattcagta cacaaacatg
    36541 atagtcaacc aaaaaatcac tcatcttttg agcattcgat ataaaaaatt ggcatactca
    36601 taaaaagggc cagcctttcg gcaataagga ataattttat aaattgtgaa aaactatttt
    36661 tcaaaattgc ttcttatcgc ttaaagctgc acgtctcatt tacaaccttt tctttttaga
    36721 tgttatgagt tgctcagata agtgattttg tttactctaa gaaaaaaggg aggcaaaaga
    36781 tacatgaatt actttatcag tgatatgcac tttttccatg aacgcttatt aggaaaaaac
    36841 gactttgctc cgcggctctt tgcaaatgtg gatgaaatga accaacagtt gatcactagc
    36901 tggaatgcag taataaaaga aaccgaccat gtttatcatc taggcgacct tgctatgcat
    36961 ccgcaatatg aaaaagcaaa tgcagaaatt ttggaactgg tcactcaact gcaaggaaca
    37021 atccacttga tcaaagggaa ccatgacagt cgcgcttttt tccgttattt agaaaaaaat
    37081 gatcctaggc ttactgacgg aagtcccaaa ttttattttt atgatgtagg tgccatcgtt
    37141 aaattcgacc atcaacaata ttacttgact cattatccga tgttgctcgg gcctaacgac
    37201 actatccgca atcttcatgg acatatccat cattacagcg tacctatcga gaacgatgtc
    37261 aatgtaggcg tagacgcacc ggaaagagaa ctcttaaagg aaaaactacc atttggcact
    37321 ccgttatctg agcaacaaat caaagaaatc tatgaagcaa agaggaagta cgataggtcg
    37381 tgaaaaaact gccctatatc aagtattaaa caaataaaga taattactca tatttttcat
    37441 ttgagtaggc ttattgccac aggaacatta ggatcagaaa tggagttgta acaagacttc
    37501 tgtcacaact ccattccata ctattcgtct ttttgctcat ggtgttcttt taccagtttt
    37561 ttctgtaatt ctttatcata atttcgggca gctgacgtta aaaataaaac gattccgcat
    37621 aataaaatcc cgattccgga aaacaagaac attttttcta cccctaaacg atcagctaat
    37681 ggtcctgcaa ataataatcc aattggacct gtcaggttca tcaaagagtt gaagttccct
    37741 aagacacgac ctaaaacatt cgattcataa ctctgctgga tcattgccat cagcagtgta
    37801 ttgaaatatg gcgttgcaca acctgctcct gcattcaaga taagaaagta caagaaccct
    37861 tgactatcgt ttggaaccaa tccgcttgca ccgatcgtca ggcccataat gatgtaggct
    37921 ataataatag gtttcattcg gtctttccat gttcctataa aactgatcag tgcaccgcct
    37981 aagagcattc cgactgcgta gactacttcc actgctcccg cctgtccaac ggaaccacca
    38041 aagtaatcta gcgtcatcaa tggatacaga ctgattgctg gcatgaacaa cagcatcaca
    38101 aatgcaccat tgatcgttat gtaccataac cctttattat ccatcaattg ctgcagccca
    38161 aattttgcat ctttaaaaat agtaagcgga acttccaata tttcaggaga aacttttggg
    38221 atcttcacaa aaatcaataa gccgacacca aaaactgccc ccaatacatc taataaaata
    38281 aggtaattga caggaacgac tgaaaacaaa gccgcaccta gagctggcgc aatgatgaaa
    38341 ttcgctgatt ggaccattcc taactgtcca ttcgtccttg ttatgtgaga agatggaacg
    38401 attgtcggca taatcgactg gatcgttggc atttggaatg tttgtgcgac cgaacgcata
    38461 aacaaagaca caaaaactag ccaaatcggg aaggattctg aaatcgttcc gactactgct
    38521 aaaataagcg cgaaaatcgc tacaataata tccgtgacaa tcaaaagagc ttttttatcc
    38581 cagcgatcca ctagcggtcc tacaaatggg cttagtaaaa ccatgggaat cattcctagt
    38641 aaagtagcaa aactcaaaat tgtcgccgaa ccggtttctc ttgtcaaata ccagatgatc
    38701 gcatattgga cgaccatact agtgattcct gacaaaaact gtcccgataa aaagaacaaa
    38761 taatttctct tccaattcgt taaaacgacc gactgttcat tttccataaa catccctctt
    38821 ttctttttca ttttgtatag cgtttgcaca acaatcatta tacgggattc ttttttcaaa
    38881 aaataccttt ttccctaaat aacaatcgtt tcaatttaaa tttctttact tagaaacaat
    38941 aaatcagcaa aatattaaaa ataacaacat gtgttaaatt taaaaataaa aaagacaact
    39001 aatttgcgtc gtccttacaa agttagtcat ctaacaatag gccgtttcaa aactagttgt
    39061 ctttttatat ccacagatca ttcgactttt tcgaataacc cacggctaat catcccatcc
    39121 atcagaaaat aaaatttatc ttggctcatt ttctgttttg gatctttgaa aatattgact
    39181 gctttcaatc catttgctgt agtgatcgac attttcaatg tttgaatatc ttttgataca
    39241 gtgattttta atttgaatcc ttctttgctt tgcggcgtgg catctaatgg tcggatcaat
    39301 tgataattgc ctccgtttgt ttctgtaaat ccattatcgc gcaatgtata tctttttgca
    39361 tcaggatgca aacgatagct aattggtgcg cccagtaccg ttgctgttgt ttcaaatgcc
    39421 atgattttca cctcgataaa attagtttct ctttattata aaagtttatc ctgattttta
    39481 ctaggttttt cagttaagtt ccctaaaaaa tgaaggcgtt ctctcagaca tctgttcata
    39541 taagaatgac ttcactagtt attttttgac agcacgaact aattgatcaa ggaaatactg
    39601 gatgtcgtct tcggtatttc ccaaaccaaa gctgatacgg atggattctt ttagtatcgg
    39661 tgtatcttct ccatacattg cttccaatac gtgtgaaggc tcgatatttc ctgctgtaca
    39721 agctgaacca gtagaaatag cgaccccatt aagatcaagc ttcatcagta gcaaatcatt
    39781 tgcttcgcca attaagcgaa gattcaacac atgagggagt ttattcgtta aatcaccatt
    39841 tacttgataa gggatttcgt ttgcttctaa tgtatcaaga atcaattgct ggaatccttt
    39901 atatttgttg ttacgtgctt cacgttcttc aggtgacagc agttcaactg cttttgccat
    39961 accccagatc cctgctaaat tttctgtacc tgcacgccgc ttttcttcct gctccccgcc
    40021 taaaagcaat gcagggatat ttactccgtc tcgtttatat aagaaaccga cgccttttgg
    40081 cccattgatt ttatgagcag agatacttaa taaatcgatt cctaattctt ctggatggat
    40141 cgtttggttt ccgtacgctt gtaccgcatc tgtatggaaa taagctggat gatccgctaa
    40201 aagttcaccg atttctttga ttggcatcaa atttccgatt tcattgttcc cgtacatcac
    40261 agagaccaag atcgtatctt ctcgtaacgc ttttttgacc tcttcgatcg acagattccc
    40321 tttttcatct acaggcagat atgtaacgtc aaaccccttt gtctctaaat atttcatggt
    40381 attaagaaca gcaggatgtt cgatagctgt agtgattaga tgtctgcctt ctttttgacg
    40441 cgagaacgct gtttctaaga tagctgtatt atccccttct gttcccccac tgttaaagat
    40501 gatctcgtga ggttttgctt gcagactgtc tgcaatgatt tgtcttgctg tttccaattt
    40561 ttcatgcgcg tggcgaccaa atccatgtat gcttgaagga tttccaaacg tgtcttgcat
    40621 gattgccgtc atcgcttcaa ttacttgtgg atgcatgggg gtagttgccg catgatccag
    40681 ataaatagtt ttcacttcat tcatcccttc caaattattg ctaggcacat tctagcataa
    40741 aatagcgaac aaaccaaaga agagctcatt tttgtctaga ggacgtttca tgggcataga
    40801 aaaaaggagc tgtgacaaaa gtcttgtcgc agctccttga agctgctcac ttctgtcaca
    40861 acttctt
//
LOCUS       DAJSBN010000024        38697 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJSBN010000024 DAJSBN010000000
VERSION     DAJSBN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.024"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(345..491)
                     /locus_tag="ONT86_001368"
     CDS             complement(345..491)
                     /locus_tag="ONT86_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HCR2352172.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(595..906)
                     /locus_tag="ONT86_001369"
     CDS             complement(595..906)
                     /locus_tag="ONT86_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2352173.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(908..1105)
                     /locus_tag="ONT86_001370"
     CDS             complement(908..1105)
                     /locus_tag="ONT86_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HCR2352174.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1499..3226)
                     /locus_tag="ONT86_001371"
     CDS             complement(1499..3226)
                     /locus_tag="ONT86_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCR2352175.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(3219..4412)
                     /locus_tag="ONT86_001372"
     CDS             complement(3219..4412)
                     /locus_tag="ONT86_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2352176.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4599..5243)
                     /locus_tag="ONT86_001373"
     CDS             complement(4599..5243)
                     /locus_tag="ONT86_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2352177.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(5337..5471)
                     /locus_tag="ONT86_001374"
     CDS             complement(5337..5471)
                     /locus_tag="ONT86_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCR2352178.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5473..7605)
                     /gene="feoB"
                     /locus_tag="ONT86_001375"
     CDS             complement(5473..7605)
                     /gene="feoB"
                     /locus_tag="ONT86_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCR2352179.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7602..8075)
                     /locus_tag="ONT86_001376"
     CDS             complement(7602..8075)
                     /locus_tag="ONT86_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCR2352180.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8476..8700
                     /gene="nrdH"
                     /locus_tag="ONT86_001377"
     CDS             8476..8700
                     /gene="nrdH"
                     /locus_tag="ONT86_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR2352181.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8859..11018
                     /gene="nrdE"
                     /locus_tag="ONT86_001378"
     CDS             8859..11018
                     /gene="nrdE"
                     /locus_tag="ONT86_001378"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR2352182.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            11068..12033
                     /gene="nrdF"
                     /locus_tag="ONT86_001379"
     CDS             11068..12033
                     /gene="nrdF"
                     /locus_tag="ONT86_001379"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR2352183.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(12122..13261)
                     /locus_tag="ONT86_001380"
     CDS             complement(12122..13261)
                     /locus_tag="ONT86_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2352184.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13570..14283)
                     /locus_tag="ONT86_001381"
     CDS             complement(13570..14283)
                     /locus_tag="ONT86_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR2352185.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14537..15238)
                     /gene="nagB"
                     /locus_tag="ONT86_001382"
     CDS             complement(14537..15238)
                     /gene="nagB"
                     /locus_tag="ONT86_001382"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR2352186.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15472..17004
                     /locus_tag="ONT86_001383"
     CDS             15472..17004
                     /locus_tag="ONT86_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2352187.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(17195..18397)
                     /locus_tag="ONT86_001384"
     CDS             complement(17195..18397)
                     /locus_tag="ONT86_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2352188.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(18479..19168)
                     /locus_tag="ONT86_001385"
     CDS             complement(18479..19168)
                     /locus_tag="ONT86_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR2352189.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(19274..19642)
                     /gene="rplL"
                     /locus_tag="ONT86_001386"
     CDS             complement(19274..19642)
                     /gene="rplL"
                     /locus_tag="ONT86_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR2352190.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19695..20198)
                     /locus_tag="ONT86_001387"
     CDS             complement(19695..20198)
                     /locus_tag="ONT86_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR2352191.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(20402..20529)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(20608..21297)
                     /gene="rplA"
                     /locus_tag="ONT86_001388"
     CDS             complement(20608..21297)
                     /gene="rplA"
                     /locus_tag="ONT86_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR2352192.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(21401..21823)
                     /gene="rplK"
                     /locus_tag="ONT86_001389"
     CDS             complement(21401..21823)
                     /gene="rplK"
                     /locus_tag="ONT86_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR2352193.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            complement(22210..22449)
                     /locus_tag="ONT86_001390"
     CDS             complement(22210..22449)
                     /locus_tag="ONT86_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2352194.1"
                     /translation="MKKEYGYPTLKVICKASAGNETAIREILKFYDAYICKLCLRPFY
                     HSESGKITMQVDEELKGQIHTEMMKAILKFEIRVK"
     gene            complement(22454..22873)
                     /locus_tag="ONT86_001391"
     CDS             complement(22454..22873)
                     /locus_tag="ONT86_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2352195.1"
                     /translation="MNTSSFEHIVRIQFNALMITVIKCTVKSRNRQFARRSKREVLFY
                     ELSDTKNLECVTEDKYSCDYISFEVLNFTIQISNEKLAVALYKLSSKERDVILLRYFQ
                     SMSDQEIAELYHVSRSAIYRRRSNGLKKLKTLLKERN"
     gene            complement(23114..23518)
                     /locus_tag="ONT86_001392"
     CDS             complement(23114..23518)
                     /locus_tag="ONT86_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HCR2352196.1"
                     /translation="MKKPKVAFVCVHNSCRSQIAEALGKYLSSNVFDSYSAGTETKPQ
                     INQDAVRLMKEMYQIDMEKTQYSKLLSEIPQVDIVITMGCNVNCPTIPCKYREDWGLN
                     DPTGKNDDEFLKTIHSIHAKILELTEKIKIMR"
     gene            complement(23548..23880)
                     /locus_tag="ONT86_001393"
     CDS             complement(23548..23880)
                     /locus_tag="ONT86_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2352197.1"
                     /translation="MKLFGKKKETKSCCCGGNCTSETMQNAENKKQEQGIKILGSGCA
                     KCMELEKATRTAISELQLDYEIEHITDFAEIASYGVMSTPALVFNGEVISYGKVLTVD
                     EVKALLSK"
     gene            complement(23897..24892)
                     /locus_tag="ONT86_001394"
     CDS             complement(23897..24892)
                     /locus_tag="ONT86_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR2352198.1"
                     /translation="MWLFFQDQILGMKWLNGLIGNGLSLLGLDINSRLGGSIQFFLYD
                     VIKITILLCLLIFFISYIQSYFPPERSKKILGRFHGIGANIISALLGTVTPFCSCSSI
                     PLFIGFTSAGLPLGVTFSFLISSPMVDLGSLVLLMSIFGAKVAFAYVIVGLIIAVIGG
                     TLIEKMHMEKYVEDFVKNASNVDISSPTLTKKDRVQYAKEQVVGTFKKVFPYILIGVG
                     IGAVIHNWIPETWIESILGSNNPFGVILATLVGVPMYADIFGTIPVAEALLSKGAQLG
                     TILSFMMAVTTLSLPSLIMLKKAVKPKLLTLFIAICTFGIIIVGYLFNIFSTLFI"
     gene            complement(24988..25293)
                     /locus_tag="ONT86_001395"
     CDS             complement(24988..25293)
                     /locus_tag="ONT86_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2352199.1"
                     /translation="MNDFSKDAKIFKALCDTKRLTILDYLKSGEKCACVLIENMNIGQ
                     SALSYHMKILCDSGIVNARQEGKWTHYSLSKSGSEYASKRLLELTTPNAENQSICCK"
     gene            complement(25521..26156)
                     /locus_tag="ONT86_001396"
     CDS             complement(25521..26156)
                     /locus_tag="ONT86_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2352200.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRTEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGTNIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYRTPYQVVKMINPNSKEDMELLREVLRNNHFDDEDE"
     gene            26666..27808
                     /locus_tag="ONT86_001397"
     CDS             26666..27808
                     /locus_tag="ONT86_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2352201.1"
                     /translation="MNNLEFALEMKNKRLASGLSQRELAGLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRLPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGTGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYKNGGAECRCKNYENVQSGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            27801..28007
                     /locus_tag="ONT86_001398"
     CDS             27801..28007
                     /locus_tag="ONT86_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR2352202.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            28086..29279
                     /locus_tag="ONT86_001399"
     CDS             28086..29279
                     /locus_tag="ONT86_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2352203.1"
                     /translation="MREKRRDNKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKRKKPSLRELEQQIRRDIEDGIDSAGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDMLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKNKEQGYYIETPKTKSGIRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRSNFLFVNHKGRPKVAIDYNALFVRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASRNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            29595..30263
                     /gene="sdaAB"
                     /locus_tag="ONT86_001400"
     CDS             29595..30263
                     /gene="sdaAB"
                     /locus_tag="ONT86_001400"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCR2352204.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            30276..31148
                     /gene="sdaAA"
                     /locus_tag="ONT86_001401"
     CDS             30276..31148
                     /gene="sdaAA"
                     /locus_tag="ONT86_001401"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCR2352205.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(31239..32258)
                     /locus_tag="ONT86_001402"
     CDS             complement(31239..32258)
                     /locus_tag="ONT86_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR2352206.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(32431..33336)
                     /locus_tag="ONT86_001403"
     CDS             complement(32431..33336)
                     /locus_tag="ONT86_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2352207.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(33395..33940)
                     /gene="nusG"
                     /locus_tag="ONT86_001404"
     CDS             complement(33395..33940)
                     /gene="nusG"
                     /locus_tag="ONT86_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR2352208.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(34041..34211)
                     /gene="secE"
                     /locus_tag="ONT86_001405"
     CDS             complement(34041..34211)
                     /gene="secE"
                     /locus_tag="ONT86_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR2352209.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(34235..34387)
                     /gene="rpmG"
                     /locus_tag="ONT86_001406"
     CDS             complement(34235..34387)
                     /gene="rpmG"
                     /locus_tag="ONT86_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR2352210.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            34549..35193
                     /locus_tag="ONT86_001407"
     CDS             34549..35193
                     /locus_tag="ONT86_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2352211.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(35249..35752)
                     /locus_tag="ONT86_001408"
     CDS             complement(35249..35752)
                     /locus_tag="ONT86_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR2352212.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(35765..35811)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            36349..37314
                     /locus_tag="ONT86_001409"
     CDS             36349..37314
                     /locus_tag="ONT86_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR2352213.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(37413..38585)
                     /locus_tag="ONT86_001410"
     CDS             complement(37413..38585)
                     /locus_tag="ONT86_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2352214.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(38572..>38697)
                     /locus_tag="ONT86_001411"
     CDS             complement(38572..>38697)
                     /locus_tag="ONT86_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2352215.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT        12024 a         7814 c         6759 g        12100 t
ORIGIN      
        1 tacgtcataa ccatggaagt aataatagat ctgaaatcat caataagcat acataatagt
       61 aatttattta aaagcactat attagttttc ttcttttcct ttaatataaa ataacttccg
      121 attataggta tagctgcgga aacatcagta aataattcaa caaaaaaagt gagtggtatc
      181 aataaacata caaaaagata ttttcttttg ttaagatcag tattagtcat ataaaataag
      241 agaaaaaaga aaaagtttcc cacgaacatt cttaatagat aagcttttgt atcgatagta
      301 atcaagagat cccctcctga ttttaggata tcataaaaaa aattttagtt acacttttta
      361 aataatgaaa aaacacattc cactaaattt gatgccggtt ttccttgagg ttttgttcca
      421 gcacctcctt taattttttt aagctcttga tctgaacatt gtttagcatt tagtctattt
      481 ttttcttcca ttacgattcc ccctacaaac tataaatact tttatgtcac tgattcaata
      541 tacctatttt tctaatcaaa aaattttgag acatatttaa tattataagt atcattaaaa
      601 ttgagattta tctccataat ctgctcgata taaaccattt attccagcag ttcttccaat
      661 ttcactcaaa tttcttatta attcctcttg atattcagtg aatcttattt tattagtata
      721 agcagtaaaa tatagataat tcaccaatct atttattaaa ggaccgggat ctaattcata
      781 tttttgtttt tctaatttta aatatacctt ttgtaaaacc tcaagaatat cagaatattt
      841 aggatcttta cttatagata tatcattata taattcatga acaatttgct tagcattttt
      901 tttcatttta gcacttccct ggaattgctc cacctaaaaa accacctata gacattcctg
      961 caatacaagt agttgccttg gcccaatcga ccgtacattt atttttagtg caatacactc
     1021 catttccata atattttcca ctatgagtgg tacccccata gataagttgt gtctctttaa
     1081 tagacaaaat ttttaaatgt ttcataatct aatcacccct aaattaatgt ttttagacta
     1141 ttattctaac attcattaaa tttcaaaaaa caattccttc ttgaaacgta gcaaaataat
     1201 tcctgaataa gaatttaatc acttttaagt atataaacta tgtatattct ttaatatttt
     1261 ttgagaatgc acttcgtaca taataaactt taatcacatt agtctttcat acctacgatc
     1321 gatctctttg actcgcaccc ataaattcat aatagtttgt cggcgtcttt ggacgtttcg
     1381 aagatgagct acgcattctc tttttttata gacaaaaaag atagagacga acatggagct
     1441 tccaaatttg gatatttgat ccaattttgg aagctccatt tatttcgtgt ctgtcttttt
     1501 attttttcca gctttcgact aattcttggt tctctttgat ccagtctttt gctgcttgct
     1561 gtggatcttt tccattgttg atttccaaca taactttttc catatctttt tctgaccaat
     1621 ggaaattgtc taatactttg taagcttctg gctggtcttc tttcaatcct tttcgaacca
     1681 tcgtatggat ctgctcttcg cttcccattc cgttttcagg atcttctaag tatttcagat
     1741 catatttggc aaacatccaa tgaggtgtcc atcctgtaac tacaattggt tcgtgttttt
     1801 tgatcgcttg acctagtgca acggtcattg ctcctgaaga agatgtctct actttccagt
     1861 ctttcaagtt gtcatatttt tggatcgtgt tttcagcagc cgtaactaca ccagctcctg
     1921 gctcgattcc tatgattttc ttacctgcct gatcggttaa ctcatcgatc gaatttacat
     1981 ccatgtaggc tggcactgct aaaccaactt ttgctcctgt caaattggca cctaggtctt
     2041 cgacttgatc tttatattgt gcatattgtg aaccatgagt tttaggtagc caagctgaaa
     2101 ccatggcatc tgactcgcca tttgaaacag atttccacat gattgaatta tctaatggcg
     2161 tcatcgttac atcatagccc atttgtttca atacttcacc gactacattt gttgaagcaa
     2221 cttctgtatc ccattcaaca taagataagt tgatacgttt tgtttctttg gcagatgaaa
     2281 aactccctat accaatcgca cctaagatca ctactactac agcaccaatg atcagtcctt
     2341 gacgtttttt acttttctgt gttgttgctc ctgcagcttt tttattgttt ggctggttca
     2401 aatgctgtgt aaaacggtca atgatgattg ctaagataac taatgcaacc ccgttgacaa
     2461 atccattccc gacttgtgct ctttgcaatg ctgataatac gccgcgtcct aagccgggtg
     2521 caccgatcat agaggcaata acgaccattg aaagtgacag cattgttgtt tgattgatcc
     2581 ctgccatgat cgtactttta gctaaaggca attctagttt gaatagtttt tgacgacttg
     2641 tgctcccgaa tgaatcagaa gcttctacta attctttagg aacttgtcga atccctaaat
     2701 tcgtaaaacg aacggttggt ggcagagcaa agatgactga ggcaaatacc cctggaacca
     2761 tcccgatacc gaaaaatgca actgctggaa tcaaataaac gaatcctggc attgtctgca
     2821 taaagtcaag gatcggtttg ataatacttt gggctttact gctttttgcc atcaagatcc
     2881 ctaatggtac cccaatgatg atcgagatca cactagataa aagaacaagt gtgacagtat
     2941 tcatcaaatc attccataga ccttggttat aaataaacca tagaccgatt aatgtgaaca
     3001 acgtaagtcc tggacgtttc ttagaaataa agaaagctgc taccgtcaat aacaagataa
     3061 acaaaggagc tggaatcact aacagtaaat ttgtaatccc cgacatcact gcctgtccaa
     3121 tcgtttgtaa aaatgaaaat aggccagaaa aagttgtcgt aaaccattct gtgatatttt
     3181 ctacccagtt tgccactggt aattgatagt tcaatagatt attcatgttc actcacctcg
     3241 cttgtttctg ctaatgcttc aatcacacta cctcggatga cgacaccttt taattttcca
     3301 tcctctacaa ctgcaagtgg agtcggtgag tcataaataa tattgaaaat atcacttacc
     3361 accatgtctt tcccaatcac cgtcacattt tcatctagga agtcgatcaa tggacgtttt
     3421 tgcttcctag cttctaacgc ttgatctgca gtgatgatcc ctttcaggtg tctctttctg
     3481 tcaacagcca tcagcatgct gacttcttca ttacgcatcc ttgtcaatgc aacgtttggt
     3541 ccatcagatt cgatattcgt cgtcaatgca ggtaccatga tgttttgagc cgtaagcact
     3601 ttggaacgat cgacttcttc cacaaattca cgtacataat catttgctgg attggtcaat
     3661 atttcttcac ctgttccgat ctgcatgatt tcgccgtctt tcatcaaggc gattctgtcc
     3721 ccaatccgta aagcttcatt caaatcatgg gtaatgaaaa taatcgtttt ttgaacattg
     3781 gcttgcaggt ccagtaattc gtcttgcatt tctcgccgga tcagcggatc aagagccgag
     3841 aacgcttcgt ccatcagcaa gatctcagga tcatttgcta aagctctggc taggccaacc
     3901 cgttgttgca tcccaccaga taactgacta ggatattggt ctttaaatga aagcaaacta
     3961 gaattttcta atgctttttc tgctttttct tgtcgttctt ctttaggtac accacggact
     4021 tctaacccgt attctgtatt ttccaagatc gttcgatgtg gaaacagacc gaagttttgg
     4081 aaaaccatat tgattttatg acgacggact tctcgcaatt cttctttgct taacttcgat
     4141 acatcttctc catcaatata gatactgccc gaagttggct caatcagacg attcaataga
     4201 cggatcaatg tggatttccc actcccggaa agccccatga tcacgaagat ttctccttct
     4261 tttacgtcaa aattcacgtc atacacacca accgttgcac cggttttctt taaaatctcc
     4321 gttttcggct ggtgatcatt catcatatct aaagcggctt gtgtcttttt accaaatatc
     4381 tttgttaaat gttttacttc tacttttacc aaacaaattc tcctttttta tgtaaattca
     4441 tttttctttc tgaaaaaagt gaccacacta tgttaacatc atgtagtcac tttgtcaaaa
     4501 atgaaagaaa tttgcttgtc caaactgaaa aaaacttggc taaatctttg taaataaaag
     4561 gtttagccaa gttaatattt ttttaatatt tcacacaatc attctgcaag ctgatgctcc
     4621 gggtaaaaaa agtttgtcat acgctgtagt gtatattcat ttccaataaa atgaacgatg
     4681 tcccctgccg taaatttagc ataggggcca ggtgaaacaa gcagttcttc attatgagaa
     4741 attgcgataa ctgtggcgcc agtcgcctgc cagatattca actcgctaat tgaacggtcc
     4801 agatgcattg ctttttctga taaagataat tcaaacggaa gtaaaggatt catcttatgt
     4861 attcgcttcg tttgattgac cagctgatcc aataattgag aaaaatgatt cagctcatct
     4921 tgttgcttct ccacacttgc catgatttct gcttttgtat cttggatgga ttgtacatct
     4981 tgatattgca ataggaaatc ttttgctttt tccttcgaag caacaaatgc cccacttcca
     5041 tgccgtactt ccatgattcc aagatctacc agcacattga tagccttgcg ggtagtctca
     5101 ggagaagcat tgtacgtact ggcaagtgtt gagcgggcat ggatcttctc acccacttta
     5161 tatcggtttt caacgatacg ttcggctaaa tcaacagcaa tctgctggta gcgcgggcgg
     5221 acgatttttt tctttccaga cattttcttt cctctttctt tttttacttt ccctcatctt
     5281 actatgctct agcaaaataa gcaaaaaagg ggagaatagc ctcttgcaac gactgatcat
     5341 cgcttgatct gttccttttt tactgggcaa gctgtttgac agtcatcaca attcttaccg
     5401 gattttaaac gagtatagac aattgcgcct gcacttccaa agattaacac acttaataag
     5461 aatgtcgcca tcctattcct ctccttctaa tgacaatata gtataaaact ggttcttgga
     5521 ttttggctga cggaacaaaa cataaacgag tcctatcatc agtaaaatag caagatacgt
     5581 ccctaacgct acctgtccac cctccaataa gtgtcccaac tgataaatca caaaactaac
     5641 taaataagct aaaccgcatt gatagcctat ggcgatccaa gtccatttct tggcattcat
     5701 ttcccgatgg atagccccaa ttgccgcaaa acaaggcgca caaagtaaat tgaaggctaa
     5761 caatgaataa gctgctgcgg gagtatatgt gtgttgtagc acttgccaga tttctctgcc
     5821 attttcggat acttccgcat gaccaaacaa aataccaaat gtgccaataa tattttcttt
     5881 agcgaccaat cctgtaatcg ctgcgactgt tccttgccag tttccccaac caagcggctg
     5941 aaaaacaact gccaggattc gcccgaaaaa ggctaagata ctttggtctt cagaaacagg
     6001 ttgagcataa aaattataag aagagctgaa ccagatcacg atactagaaa caaagataat
     6061 cgttcctgct tttttaacaa aagacttgct gcggtcatag gtttgtctta ggaccgctga
     6121 ccattgagga aaatgatatg cgggcaattc catgataaaa ggagctgtat ctccagcaaa
     6181 caaacgtgtt tttttcaagc tgattccaga gaaaataatt gcagtcatac caagaaaata
     6241 ggcagaagga gctacccaag attgatgagg aaaaaaggcc cctgctacta aggcaataat
     6301 cggcaacttt gcagaacaag gcatgaaggt tgtgaccatg atcgtcaaac gacgatcttg
     6361 ctcgttttca atcgtgcgac tggccatgac gcctggcact ccacatcctg tagcaatcag
     6421 cataggaatg aaggattttc ctgaaagacc gaatttcctg aaaagtcgat ccatgacaaa
     6481 agcgatcctc gccatatatc cgcagtcttc cagaaagctc aagcataaaa acaatataat
     6541 caattgcggt agaaatccta acactgctcc cacgcctgcg atgatgccgt tcaagatcag
     6601 ctcctgcatc catcccgcta cttgccattc agctaacaat ctgccaacat gttctggtaa
     6661 ccatgtacca aacaatgtgt cattgatcca atctgtcccc attgttccta ctgtttgaat
     6721 agccagataa tagatcagcc acatcacaaa tgcgaagatc ggcaaggcta accaacgatt
     6781 cgttgcaatc tgatcgattt tgtcactcat cgataattgg aaagaatcat ttgacacaac
     6841 acagagtgta catagttggg taataaattc ataacgttca ttgaccagaa tcgtctcact
     6901 atcgtcgtcc aatagtttct ctgttaactg gatcagttgc tcgatctctt tttgctgaga
     6961 aactgctatc tcgtaatcct ctcttacctt ctgatctcgt tcaaaatatt tcattgcata
     7021 ccagcgtttc ttgccgcttt gttctcctag tacatcttca atttcatgta aagctgcttc
     7081 taaacgtgga tcatatgtac agggaacagg aatagaaggc cgcttttttg cagactcctg
     7141 aatcttttcg attagttttt cgattccttg ctttttcaat acgctgattg gtaaaactgg
     7201 tacaccaaga ccataggaaa gcttttctag attgattttt tttccttctt ttttgatgac
     7261 atccatcata ttcagaccaa tgatcatagg aatgtctaaa tccaataact gcgtagttaa
     7321 ataaagattt cgttctaaat tggtagcatc aataatgttg atgatcagtt caggctgttc
     7381 ctgaaccaaa taatctcttg caatcacttc ttctggtgta taaggagaca aagaatagat
     7441 gccgggtagg tcctgaatat gaatgttcgg attttttttc aacgatcctt cttttttttc
     7501 tactgttacg cccggccaat tccctacata ttgattcgaa ccagtcaaga gattgaacaa
     7561 gctagttttc ccgctgtttg gatttccgac taacgcgata ttcattcttt ttcctccctt
     7621 tgtatcaaga ttagttcggc ctctgacttt cgtaacgtca gttcataccc tcgcactgtc
     7681 agttcgattg gatctcccag aggggctagt tttcggataa ctatttttac atttcgcgtc
     7741 aatcccatat ccattaaccg tcgcttcact gcgccttgtc cgtggattgc tacgacgatc
     7801 ccagtctctc ctgcttttaa ttgatcaaga gaagtccagc tttcagagga agcagtcttt
     7861 ttattgacaa agatttgacc tagtatttcc cgacttactg ccactcgatg actttttacc
     7921 agtaaaatgg cattgttgcc tgataaattg acgacgacga ttttacttcc ggcaacgatc
     7981 ccgatatttt gcaaatggcg ttttgttttt tcttctgttt cgattcgttc gacttgataa
     8041 acgaaattct gctttgcctg cgctaaggtc tgcatattga ttcctctctt gtgaaataaa
     8101 tcatgtatta aaatcattca ttatatgttc taattttctt acgtgttacg caaataaatg
     8161 agaatcattc tcacatagaa tactctcggt aattgagaat gtcaattata cttgtgatat
     8221 tatgagcgaa tctttagtat tgatagataa aaatgagaaa aaaatatttc ttcatagttt
     8281 tccacaattt tttctctatt aaatttgcta ttttcccaca cgatacttgt tttcagctct
     8341 tttttcaaaa acacaaaact tagtattcca cagtttccac aacacaatat gtagtgtttg
     8401 ttgtttgact tttttactaa gagttttata ctatctatta tagataataa gaatgtgtta
     8461 gaggaggcac tcaccatgaa cgtaaaaatc ttttctaaaa ataattgtat gcaatgtaag
     8521 atggtcaaac ggtttttaac agaaaatcaa attgcttttg aagaagtcaa tatcgatgaa
     8581 cagcctgaag cagtagactg gttgaaagca caaggattcc aaagtgttcc tgtcattact
     8641 tctgatgcag aaacagttat cggattccgt cctgatcagt tgcgtaaact tgccagctaa
     8701 catgactaaa agaatcaaaa cgtgatacat ggatttgaag ggcttgagac agcaccagtt
     8761 tttgtcgcaa acttccaaat ccttttttaa agcttaaaaa caatgcaatg atttgcaatc
     8821 aagcatcatc aaatacgaaa tgaaggttgg gcgtacgtat gagtcttaaa aatttacaag
     8881 atgtcagtta tttcaagctg aataatgaaa tcaatcggcc agtcaatggt caaatccctt
     8941 tgaataagga taaagaagct ttagttgcct tttttgagga aaatgtcaaa ccaaatacga
     9001 tgacttttct atctattatg gaaaaaatca attacttgat cgatcaagaa tatatagaag
     9061 cagaattcat tcaattgtat tcaccatcat ttattgaaaa gctatatcaa tttttggacg
     9121 agcaacattt ttctttcaaa tcttttatgg ctgcttacaa attttattct caatatgcgt
     9181 tgaaaaccaa cgacggttcc gcttatctgg aaacttatga agatcgagta gccttcaatg
     9241 cgctttactt tgcgaacggg gatgaagaat tagcacttgc tcttgcagac gaaatgatcc
     9301 atcaacgata tcagccagct acaccttctt tcttgaatgc tggacgtaaa cgtcggggcg
     9361 aattggtgtc ttgtttcttg actcaagtca ctgacgatat gaactctatc gggcgctcaa
     9421 tcaactctgc tttgcaattg tcccgtattg gtggcggcgt agggattact ttatcaaatc
     9481 ttcgtgaagc cggcgctcct atcaaaggat atgagggtgc tgcaagtggt gtcgttccag
     9541 tcatgaaatt attcgaagat agtttcagct attctaatca attaggtcag cgtcaaggag
     9601 ctggcgtggt ttatttgaac gttttccatc cagacattat tgctttcctt tcagcaaaaa
     9661 aagaaaatgc agatgaaaag attcgcgtaa aaactctttc tcttggcgtg atcgtacctg
     9721 ataaatttta tgaattgaca cgtaaaaacg aagatatgta tttatttagt ccatatagtg
     9781 tcgaaaaaga atatggtgtc ccattctctt atgtaaatat tacagctgag tatgatcatc
     9841 tagttgcaaa tccaaacatt cgcaaacaaa aaatcaaagc gcgtgatctt gaaaatgaaa
     9901 tctcaaaatt acaacaagaa tctggctatc cttacgtaat caatattgat acagcgaacc
     9961 gcgagaatcc aatcgatggc aagatcatca tgagtaattt atgttcagag attttacaag
    10021 ttcaaacgcc ttctgtgatt aacggcaaac aggaatatga agtattagga acggatatct
    10081 catgtaactt aggctcgaca aatatcgtga atttgatgca aagccctaac tttggtcaat
    10141 ctgtgcgtac gatgactcgt gcattgacat ttattacaga tgcatctgat atcgatgtcg
    10201 taccaccgat ccaaaacgga aataagctga atcatacgat tggattaggt gccatgggct
    10261 tacacactta tcttgcaaaa gaacaaatgg aatatggctc agaggattca ttagacttta
    10321 caaatattta cttcatgctg ttgaattatt ggacacttgt tgaaagcaac aatatcgctc
    10381 gtgaaagaaa acaagttttc cataattttg aaaaatcagc ttatgctgac ggcacttatt
    10441 tcgaaaaata cgtaactgga gaattccagc caaaatcaga aaaagtcaaa gcattgtttg
    10501 aaggcatttt tatcccaact gctgaagatt ggaatgcttt gaaacaagca gtaatgaaag
    10561 atggactgta tcatcaaaat cgcttggcag tagcaccaaa tggctcaatt tcatatatca
    10621 acgatacgag tgcaagtatt catcctatca ctcgcatgat cgaagaacgc caagagaaga
    10681 aaatcggtaa aatttattac ccagcgcctt atctatccaa tgagacgatt ccgtattaca
    10741 cttctgctta tgatatggac atgcgtaaag taatcgatgt ttatgcaatg gcacaacaac
    10801 acgtcgatca aggcatgagt ttgactttat tcatgcgttc tgaaatccca gaaggcattt
    10861 atgaatggaa gacaacaacg aagcaaacga cacgtgattt gaatatttta agacattacg
    10921 ctttccacaa aggaatcaaa tccatttatt acgtacgtac ctttactgat gatgcagaag
    10981 aaatcggaag caatcaatgt gaaagctgcg tgatctaata tcatagatag attcgcttat
    11041 tcatatttta attaaaggag cattctcatg tcagaaacat attatgaagc aataaactgg
    11101 aatgaaattg aagatatcat tgataaatcc acttgggaga agctgacaga gcaattttgg
    11161 ctagatacac gtatcccact ttcaaatgac ctagatgatt ggcgtacatt gtctcaattg
    11221 gaaaaagata cagtaggaca tgtatttggc ggtttgacac tgctagatac tgtccaatct
    11281 gaaagtggca tggatcagtt gcgtaaagat gtccgcactc cccatgaaga agccgttttg
    11341 aacaatattc aattcatgga atccgttcat gccaaaagtt attcttctat ttttagtaca
    11401 ctgaatacga aaaaagaaat cgaagaaatt tttgactgga caaatacgaa tccttatctg
    11461 caaaaaaaag ctgagcgtat caatgaaatc tacaaacacg gtacaccgct agaaaagaaa
    11521 attgccagcg tctttttaga aacattcttg ttctattcag gtttttatac tcctctttat
    11581 tatttaggaa acaacaaatt ggctaacgta gcagagatca tcaaactaat catccgagat
    11641 gaatctgtac atggaaccta catcggttac aaattccaat taggattcaa cgagttacct
    11701 gaagcagaac aagaaacgtt aaaagactgg atgtacaatc ttctttatga gctttacgaa
    11761 aacgaagaac gttatacaga agaattatac gatccaatcg gctggacaga agaagtcaag
    11821 acattcctac gctataatgc caacaaagca ttgatgaacc tagggatgga tccactcttt
    11881 ccagatactg ccaatgatgt caacccaatc gtaatgaacg ggatttcaac aggcacaagc
    11941 aaccatgact tcttctcaca agtcggaaac ggctatttgt taggtcatgt agaagcgatg
    12001 aaagacgacg attatttgat tggattggaa taaggattta ttttaattca aaaatttttc
    12061 gaaaaataaa aagagtttag cgtacagata ttttaaatgt acactgaact cttttttatg
    12121 cttattcact tgtttcttcc agcacttttg gactgtctaa cttttccgaa acatgttcca
    12181 ttcttctcaa taaatagatt ttccggatat ttaatattat cgtcttaatc actgcataga
    12241 caggtatgca aatcagcatg cctaatattc ctgcgacact tcctgctcca atcaatacca
    12301 agatgatcgt caaaggatgg atattcattg atttcccaaa taacaacggc ttgatcaaat
    12361 ttccatctaa ttgttggatc actaaaatcg acaacgccat atacaaagct tgaaatggag
    12421 agataaacaa accgacaatg attgctggta ccgctccaat aaaagggccg acatacggga
    12481 taatattcgt gattccgcaa atgaccgcaa gcaacaagct ataaggctga tcgaatattc
    12541 tcattgccaa aaagctcatt acaccaataa tcaaagcatc taagatcgta ctgctgatat
    12601 aggcagataa tgtttcgttt aattctttta ccgtttggcg taactcttta cgaatcgctt
    12661 tcggaaagaa gtgagacaac gcatcaataa atttgtgccc atccttgaac ataaatagca
    12721 aaataaatgg aacggtaaat aacaaaataa aaaattgcat cataaatgaa atgatttttg
    12781 agacacttgc agataaactg actaatatga tattgaacaa attactaaga gataaatttg
    12841 ctgatgctaa ttgttcttcc aaattgaaat ttttaaactc ttcccgctgc gccaattcat
    12901 ttaaccattt gcttgtttca tctgcataca ttggcaagct ctgggtcaat tgaattgtct
    12961 gttccattaa ctgaggcact acattcatga cagttaaaac aactaaaatg attacgacca
    13021 aaaacgtcaa taaaaaaccg aacactctcg ggatccgatg cttttcgaga aaaacaacga
    13081 ttggatcgaa catataatag ataaaaccag caacaagcaa tggtaaaaaa actgccgaaa
    13141 gcattttcaa tattggacta aaaatattcg gcatttgtaa aataaagtac acgccgatga
    13201 tcgtgagtac tagctccact gtccagaaaa ataattttga ttgtttaaaa cgtgattgca
    13261 tacatctcct ccaaggtcct ttgatacttc tattctatca tttaacaaag agttacgcgc
    13321 tgagattttc atttgcttaa aaaaaacaaa aaaaataaac atattttaat gagtagacga
    13381 gtacttaaac aaaaacacgg tttggagtag tgacaaaaat tatactgcag tccctttttc
    13441 caaataaacg gtgttcaaaa gcttgcatct tcgtaataag cccagccaaa cgaaaaatat
    13501 aaggaactat ttttatttgg ctgggcttat tacttgatgt aggacatcta ttttacaacc
    13561 tcagatcatt tattgcgcaa tactgactaa atgcaacttt gtgatatttt tgtgtacgga
    13621 caactcctcg atcacatcgg cataagtgag cccttttgga agatcaatcg tatagatatt
    13681 tttgtacact cggtagtcat caacgaattt tacgtcaaaa ttcacatctt cgattacgat
    13741 atttttctct tcaaaataat cggtaagaaa atctttggtt tcttttctat ggataaattg
    13801 gatttctaac tttttagtcg taggtacatg gatcacgcgt ttcactactg tgagtgcaat
    13861 agcaatcccg ataaaactga tgatcgcaat ggaatagtaa cccatgccga tggcgatccc
    13921 taatccagca attgcccata aagaagcggc agtcgtaagt cccgtcaccg attgtttcgt
    13981 gacgatgatc gttcccgcgc ctaagaaacc gacaccactc accacttgag caatcagcct
    14041 tgcttcatct gaacgaatcg ttccagcaag ctgtggatac tctaaagcat ctcttaatgc
    14101 accactagcg atctcaactt ggatcaatgc aatgatacat gctcccatac aaactaaaat
    14161 atgcgtacgc atacctgctg gacgattttt atattgtcgt tcaaaaccga ttgctccgcc
    14221 aaatagcata gcgatcccta aacgtaaaat aatctctgga attgtcaata ccatatcatt
    14281 catctaaaca ccctccagtc caatatatca tgtctgaaag gagcagacaa aaaaagtgca
    14341 atttttacct cttacgaaaa aaggactgtg acaaaatttt tgtcatagtc ctttttatga
    14401 ataaacggtg ttcaatcagc tgaccctcgg tattactcaa caaacggcaa aatatgggga
    14461 gctatttttc ctttgtcttc tctaatactc aggtcaaaac gctgatttca caacctcttt
    14521 agcctactat tgaaaattac aacttacttg ctgcttcttc atcgatgatc acaacaacat
    14581 tgtcgtgatt ttgcaataca cttgctggca aatgattcgt tacaggtcct tcaatcatcc
    14641 ctttgattgc atcagctttg ctttcgccaa atgccatcaa gacgatttct ttacttttca
    14701 taattgaacc gattcccatt gaaacagctt ttgttggtac atcttctgct ttatcgaaga
    14761 atcgtttgtt tgcattgatt gttgattctg ttaagtcaac tactgaagtc gttccgtcaa
    14821 atgaagcacc aggttcattg aaaccgatat gtccattttg cccgattcct aagatttgga
    14881 tatcgattgg atgtgcatcg atgatttgat catacttttt gcatgctgct tccagatctt
    14941 ctgcttttcc atctggtaca aacgtttctt tgaatggttt tttatcaaat aattggacat
    15001 ccatgaaatg acggtagctt tgttcatctg aaccgcctag tccaacgtac tcatctaagt
    15061 ttacagaagt catttcactg aaatccaaat cgcttgaagt catttcttta tataaagtaa
    15121 ctggtgtgct tcctgttgcc aatcctaata cttttgcacc tttgttcata ccttctttga
    15181 tgatttcaaa tgcttttttt ccgccttctt cagcgttttt tacacgaatg atttccatag
    15241 tgattgctcc tttatatttt ggtatagccc aattatagca ttcttttttt ctttttacaa
    15301 taattagtat agaccaaatt gcattttttt cattttcttg acaataaata ggaatccgtg
    15361 ttaggtttca taagatggtt caatcaaaca atccatgttg catgaaaacc aagcaagtga
    15421 ttcactgtgg acgctttatg tctgcagttt ttttatgaaa aaggagttat tatgagtata
    15481 ctgacattaa acaacattac ccagcaattt ggcgacaaaa tcctctatga aggtgtcgat
    15541 ctgcaattaa atgctggcga acatcttggg ctgattggac aaaacggagc cggtaaaagt
    15601 acattgatta aaatcatcac tggtgagatc ttaccagatg acggacagat ccattggcaa
    15661 aaaaatattc acaaaggtta cctcgatcaa tatgtggaag tcgatgaaac attaacaatc
    15721 gaagaatttc taaaaactgc gtacgcaaaa gaatacgaaa aggaagaaaa aattgctttt
    15781 ctttaccaaa catatagcga gaccttcgat gattccttgc tggaaaaagc tgggaaactc
    15841 caaacggaat tagaccaagg agtgttttat caaatcgaca ccttagttgc tgagatgagc
    15901 agcgggttgg ggatcgatgt cttaggttta gattcactgt tgaaaaatct aagtggtgga
    15961 caacgttcaa aggtgatttt agcaaaacta cttttagaaa acccagatgt tctgatctta
    16021 gatgaaccaa ccaatcactt agatgatcag catatccaat ggctgacgca gtttcttcag
    16081 gattttgacg gtgcgtatat cgtgatttcc catgaccaag aatttcttga tcagatcacc
    16141 acccatatcg cagacatcga gtttggaaag attactaagt atactggcca tctaaaacaa
    16201 gcattgaaac aaaaggaaca aaatcgcgaa agctatttgc gccaatatca tgctcaacaa
    16261 aaacaaatcg aaaagaccga agcttatatt cgaaaataca aagccggttc ccgatcaacg
    16321 atggcaaaaa gccgacaaaa acaattagat aaaatcgaac ggctgacgcc tccagcttca
    16381 gcaagtaaag ctgtctttga ttttccattc tcgcctattg tcacgacatt agcagtggaa
    16441 acaactgatt tagtcatcgg atatgatcga ccgctacttg cacccatcaa tttgaccatc
    16501 cgctttggtg aaaatattgc cattcgaggc ttcaacggga ttggaaaatc tactttatta
    16561 aaaacactga tcggtgagat tgaaaaaata agtggcgatt tccacttccc agataacaca
    16621 aaaatcaatt acttttcaca agatctgtac tgggaaaatc cattagaaac accactacaa
    16681 tatttaagca gtcaatttcc aaaagcgact atcaaagaat tacgtcgcca actagctaaa
    16741 gcagggttag ttaatcaatt agcaagtgaa cctctcacta cattaagtgg tggggaacaa
    16801 actaaggtga aacttgcaca gttgacaatg aaccaaggta atttactgat tctcgatgaa
    16861 ccgacgaacc atatcgatca agaaacgaaa gaaagcttac aagacgggat acagaaattt
    16921 ccaggaacag taatcatcgt ctctcatgaa caagaatttt atcaagatct tgtgcaacga
    16981 gtcattgaga ttgaaccaaa ataaacgttt gattgaatag ttgaaaaaaa ggagctgtga
    17041 caaaagtttt gtcacaactc cttcttctga ataaatggtg gccaattagc tgaccttcgg
    17101 tattagctca aaaaacggca aaatatgagg agctattttt atttggctat tcttattact
    17161 tgatgcagaa cagcttttca caacctcttt tcgtctattt tttattttcg agatttctct
    17221 ttcctttaaa aacaactgtt tgaaagaaga acgcacatcc ttgaaaaaca agcccgctaa
    17281 ccgttacacc taaccagcca tactgctgcc acattgtcgt ccctagcaat gaaccagccg
    17341 cacctccaat aaagtacata aacataaata cagtattatt tcggttgctt gctgcttcac
    17401 ctagcccttg tactcgtgtt tgattggcga cttgaccaaa ttgattcccg acgtctaata
    17461 aaataatagc taaaagcaaa atggccaaat ggcttccgcc aaccaataaa gcaataaagc
    17521 ttattgtttg cataacaagt cccatataca cgatttttct ttcagaataa gaatctgcca
    17581 gtcgtcccac gataggtgca gcaactgctc cagacaaacc aaaaatcgct aatatccctg
    17641 cttcgaatgt tccccaattg taggcgggac tgctgatata aaaaatcaac gtagaccaaa
    17701 agatcgaaaa tgttccaaac atgaaaaatc cactgactgc cgcttctctt aatagacgtt
    17761 gagaagcaac taaatgcggc aaacttttaa gtgaatcaag gtatgaaaca gctgtactac
    17821 tttgctggac agaacgcggc attttccagt aaagcagaag tgtaagaatt ccgactgcac
    17881 ataaagcaaa taaatagatc atccgccaag aaaaaagact ggcaacaata ccagataccg
    17941 tccgagaaag caaaatcccc acgagtaaac cgctaagcaa tgtacccatg accttccctc
    18001 ttgcttttgg tcccgctagt accgcaccgt aaggaatgat gatttgcggc acgatcgaaa
    18061 gtaatcctat taaaaaagaa gcgcatgcaa ataacgtaaa cgaaggcgca aaaaaagcgg
    18121 ccaacagaga gacggcagat aacgctgcca ttcgaatgat caattttgga cgatccacca
    18181 cgtcccctaa aggaactaaa aacagtaacc ctaacgcata gcctaattgg gtcaacatcg
    18241 tcaatatccc gactgcactt gtgctaacag ataaactagc tgctactttt gttccaatcg
    18301 gctggatgta atacatattg gcaacaacaa cgccacaagt gattgataat agtaaaataa
    18361 gcgaagttgt taaaggtttt ttttcattac tcttcattga tttcccccat ttcctatctt
    18421 agtttacaac ttatggaaaa gattggaaat agaaaaatac aactattcgc cacacttatt
    18481 atttgtgctt ttgttttcct tgttttttct tgcgatcttt ataaatgaag acaactaacc
    18541 ctactagcac agtaacactt gcaaccaacg tgactgcagt tttgttatat ccgtgaaaca
    18601 gagcttctcc accacacgca tacaacacag acgaagggat gactccaatc acgatcgaca
    18661 atatgatttg ctgtagaggc aatttcatcg catctgcagc aaaattgacc atagaagatg
    18721 gaataaccgg tatcatatag cctaacatta cacctatttt tggatgtttc atttgtcgga
    18781 ttgcttgcac ccaatgattc gtttcggttt tcttatctaa aaattttaag cgatgcataa
    18841 ggaaaattgc agtgatgtta ccaagagcat tacccaaaat gttgatgatc gcacctgtca
    18901 gtggtccaaa gctcaaacca gcaatgactc caactactga agtaggaata cctggaacag
    18961 cacacatcac cgctgtcaat acgatcaaga gtatcgatgc aaaagtccca tgcgaacgaa
    19021 tatgtgcgag tagctctgcc ttactcgctc tcggatttaa aaataattgg atgtctgtct
    19081 gatattctaa atacaaccga tagcccacta ccagtatcag aatgattccc aacgagatca
    19141 atatcaatcg gattattttc tttttcatat ttcctcctga aaaaaatcta tattcattaa
    19201 ataatagttt aacataaaaa caaaaaagca ccgaaatcca acataggatc tcagtgcttt
    19261 taaatagtaa aaattattta acagttacag atgcgccaac ttcttcaagt ttagctttca
    19321 attcttcagc ttcttcttta gatacgcctt ctttaactgg tgcaggagcg ccatcaacta
    19381 cagctttagc ttctttcaag cctaaaccag ttgcttcacg aactgctttg ataactttaa
    19441 ctttttggtc tccagctgca gttaattcta cagtgaattc tgtttgttct tcagcagcag
    19501 cagcgccacc agcagcagct acagctacag gagcagcagc agatacgcca aattcttctt
    19561 caatagcttt aactaggtcg ttaagttcta ggatagttgc ttcttttaac tcagcaacga
    19621 tgttttcaat gttcaatgcc attataattt cctccgttta attgttcttt tttatttaat
    19681 gctttatgcg gcaattaagc tgcatctcca tcttctttgt ctgccactgc tttgacagcg
    19741 taagccacgt tgcggactgg cgcttgtagt acagatagca acatagatag aagtccttcg
    19801 cgatttggca attttgccaa tgctgtgatt tcttccaatg aagagacttt accttcgatg
    19861 ataccgcctt tgatttctaa tgcttttgct tctttagcaa attcgtccat gattttcgct
    19921 ggtgcgacta catcttcgtt actgaatgca acagctgtag gtccagtgaa tacttcgtcc
    19981 atgccttcaa ggccagcttt tttagctgca cgtgaaagaa tagaattttt gataactttc
    20041 atttctacgt tagcatcacg aagttgttta cgtaaacgtg ttacttcatc aactgttaat
    20101 ccgcggtagt caacgactac tactgatgca gctgctgtaa atttttcgct tacttcatca
    20161 acgattgctg ctttttttgc gataattgct tcactcattt ttgagtttca cctcctgaat
    20221 tttgatggaa gagttttttg cactgaaaag gcaagtcact tttatagtta ctttgctcaa
    20281 ccagtattat tcatcaccta ttcacttcgt tcatagtgat tctactcgct ttgctcgaaa
    20341 ggaaactcct ttcgctttgc tcaaccagta ttgttcatca tctcttcact tcgttcatag
    20401 tgattcacaa taaaaaactc tatgccaccg cagacataga gggactattg aatatattca
    20461 ataaacgtcc tcggtaggaa attaaggctt gatgccacct actgtcttcg gtacaagtta
    20521 attattaacc tcaactagct tagcatgcta aactagttga gtcaagaatt atttttcagt
    20581 tggatcaatc aacattctaa tatttaatta gaaagaagct tggtcaacat ggataccagg
    20641 tccaaatgtt gttgtgatcg ttacattttt catgtattga cctttagctg ctgatggttt
    20701 agcttttagc aatacatcat ggattgctgc aaagttttca actaattttt catcgtcaaa
    20761 tgaaactttt ccgattggta catggatatt tccagctttg tccacacggt aagttacttt
    20821 accagctttc acttcgttta ctgcttttgt tacgtccatt gtaactgtac cagttttagg
    20881 gtttggcatt aagccttttg gtcctaatac tcgtcctaga cgacctacag tagccatcat
    20941 atcaggtgtt gcaacaacta catcaaagtc aaaccatccg ttttggattt tttgaaccat
    21001 gtcgtcgtca cctacgaaat ctgcaccagc tgcttcagct tcttttgctt tgtctccttt
    21061 agcaaaaact aaaacagttt gtgttttacc agttccgttt ggtaatacaa cagcaccacg
    21121 gatttgttgg tctgcttttt tagggtctac gttcaaacgg tatgcaactt caactgttgc
    21181 atcaaatttt gcaatgtttg tttcttttgc taaagctact gcttcggcaa caggataaac
    21241 tttacttgaa tcaacttttt ttaatgcatc ttgcattttt ttgctctttt tagccatttt
    21301 ggttcctcct tgattgtggt tataacggtt gtacctccca cgtgttctat atctttcaat
    21361 atagagccaa ctgagaagct gctgtcttag ggtgtataga ttattctaca gtgatcccca
    21421 tgcttcgtgc agttccttca accatgcgca ttgctgcttc aacgttagct gcgtttaggt
    21481 cttccatttt taattcagcg atttctctta cttgatcgct agaaactttc gcaactttat
    21541 ttttgtttgg ttcgcctgaa cctttttcga ttttagctgc ttttttcaat agaacagcag
    21601 ctggcggtgt ttttgtaacg aatgtgaatg aacggtcttc gtatacagaa ataacaaccg
    21661 gaatgatcaa acctgcttga tcagctgtac gagcgttgaa ttcttttgtg aatcccatga
    21721 tattgatacc cgcttgacct agtgcaggac ctactggtgg agctggagtt gctttacctg
    21781 caggaatttg caatttaacg attttttcta ctttctttgc cacgagacat acctccttaa
    21841 gtccgtgatg tggttaattg gagatgcata tttctcctcc cacgttttat ctgatctgaa
    21901 aaaatccaga caaataatgt gcaaaacaat gcacactggt acagtatagc aaatttattt
    21961 gttttttcaa gatttttttc tgctattttt acattttgct tgaataaacg ctaaatttaa
    22021 tataaatttt atatctcatt ttcaattatc cgcataaata ctatacattc aagtatttta
    22081 aggtatagaa aaacactcat tttaccacga atttaccacg attgaatgag ccttgatatg
    22141 acacaaaaaa caacatggga gatagcacaa atatctgtgt atctcccgtt tttcattcat
    22201 gtaatatgat tactttactc tgatttcaaa tttcaagatt gctttcatca tttctgtatg
    22261 aatttgacct tttaattctt catcaacctg catagtaatt ttaccacttt cagagtggta
    22321 aaacggacgc aagcataatt tacaaatata agcgtcataa aacttcaaaa tctctcttat
    22381 ggcagtttca tttccagcag acgctttaca tattactttt aatgtagggt agccatattc
    22441 ttttttcatt gtttcaattc ctttctttca ataatgtttt taatttcttc aatccgttac
    22501 ttcttctgcg ataaatagca gaacgtgata catgatataa ttctgcaatt tcttggtcgc
    22561 tcatgctttg aaaatagcgt aatagaatta catcacgctc tttactcgat aacttatata
    22621 aagcaacagc aagtttctcg tttgatattt gaattgtgaa attcaagact tcaaaagaaa
    22681 tataatcaca agaatattta tcctcagtta cacattcaag atttttcgta tctgataatt
    22741 catagaataa aacctcacgc ttggaacgtc ttgcaaactg tctatttctg ctttttactg
    22801 tgcatttaat aacagttatc atcaaagcat taaactgtat tctgacgata tgctcgaaag
    22861 aagatgtgtt cataatctca ccccctttct gcatacgccg aaatatataa acgggaatac
    22921 cgcctatacc ttttggtaat gtaaaaaaga aagcaagaaa aaagcccgca caaaacatat
    22981 agtctgcacg agccttcaaa atttccttat attcagatgt tgataattgc catattagac
    23041 tgtttaatag tgtgcttaaa acaatttatg acaatattct tcgttaatat tttcatttag
    23101 tttctccttg aaatcaacgc attattttta ttttttctgt caattcaagt atttttgcat
    23161 ggattgaatg gattgtcttt aaaaattcat catcattttt ccctgtaggg tcattcaacc
    23221 cccaatcctc tctatatttg catggtattg taggacaatt cacattgcac cccattgtaa
    23281 tcacaatatc tacctgtggt atttcagata acaatttaga atattgtgtt ttctccatat
    23341 ctatctgata catttctttc attagacgaa ctgcatcttg attgatttgt ggctttgttt
    23401 ctgtgcctgc ggaataacta tcaaaaacat ttgaagaaag atacttgccc aatgcctccg
    23461 caatttgact gcgacaagaa ttatgaacac agacaaaggc tactttaggt tttttcataa
    23521 atgaattacc aacctttctt tgttttatta tttgctcaat aatgctttta cttcgtctac
    23581 ggtcaatact tttccatagg atattacttc gccattaaac accaatgcag gtgtagacat
    23641 tactccataa ctagcgattt ctgcaaaatc tgtaatgtgt tcaatctcat aatctaattg
    23701 aagttccgat attgctgttc tagttgcttt ttccaattcc atacattttg cacagcccga
    23761 acctaatatt ttaatgcctt gctcttgttt cttgttttct gcattttgca ttgtttctga
    23821 tgtgcaattt ccaccacaac aacatgattt tgtttctttt ttctttccaa ataatttcat
    23881 ttcttattcg ctccttttaa ataaataatg tactaaaaat attaaagaga taacctacaa
    23941 taataatgcc aaacgtacaa atggcaataa aaagggtcaa tagtttaggc tttactgcct
    24001 ttttcagcat tattaaagac ggcaaactca atgttgttac agccatcata aatgataaaa
    24061 ttgtacctaa ttgtgcaccc tttgaaagta atgcttctgc tactggaatt gttccaaaaa
    24121 tatcagcata cataggaact ccgacaagag tggctaaaat aacaccaaac gggttattac
    24181 tgcccaagat actttcaatc catgtttcag gtatccagtt atggataact gctccgatac
    24241 ccacaccaat caaaatgtat ggaaatactt tcttgaatgt tcctacaact tgttcttttg
    24301 catattgaac tctatccttt ttggttaaag taggagaact aatatcgaca ttacttgcat
    24361 ttttcacgaa atcttccaca tatttttcca tgtgcatttt ttcaattaaa gtaccgccaa
    24421 ttacagcgat tatcaagcct acaatcacat aagcaaaagc aactttagcc ccgaaaatac
    24481 tcattagcaa tacaagactc cctaagtcaa ccataggcga agaaatcaaa aaggaaaaag
    24541 ttactcctaa cggtaatcct gcacttgtaa aacctataaa caacgggata gaagaacacg
    24601 agcaaaatgg tgtaactgtt cctaacaatg cggatatgat atttgcaccg ataccatgaa
    24661 atcgccctaa aattttttta cttcgttcgg gtggaaaata actttgaata tacgatataa
    24721 agaaaatcag taaacataac aagattgtga ttttgattac atcgtataaa aagaattgaa
    24781 tacttccacc taaacgactg ttaatgtcta gccctagcaa tgatagccca ttaccaatta
    24841 agccattcaa ccatttcata cctaatattt ggtcttgaaa aaatagccat attgaattta
    24901 ctacttccaa taaagtccct cctttacatg acatatcaat tttttttgat gtgataaagt
    24961 tttataaaag ccacctttcg atgaccttta tttgcaacaa attgattgat tttcagcatt
    25021 tggtgttgtt agttctaaca atcgttttga cgcatattca cttccacttt tacttaggct
    25081 gtaatgagtc cattttccct cttgtcttgc attaacaata ccagagtcgc aaagtatttt
    25141 catgtggtag gataaagccg attgtcctat gttcatgttt tcaatcagaa cacacgcaca
    25201 tttttctccg ctctttaagt aatctaaaat cgtaagtctt tttgtatcac aaagagcctt
    25261 aaaaattttt gcgtctttag aaaaatcatt cattattatc actcctcaca tcaaataatt
    25321 ttgatatatt tatcataagg cttacaacat gatttgtcaa cacattacat caaaatattt
    25381 tgatgtatta ttcttttttc ctcttgatta cccataacag aaaagaaaaa gccctgtcaa
    25441 gagcttgcca ccattggtgg tgctttgcac tctttactgg gctttttgtt tgctgtatct
    25501 tccatgcaag aggaaaaaga ttattcgtcc tcatcatcaa aatggttatt ccttaatact
    25561 tcccgtaata attccatatc ttcttttgaa ttagggttta tcattttgac cacttgataa
    25621 ggtgtcctat atttatgtcc ctctatgctt tccccaaaca tatccatgct gtataaatca
    25681 tcatagcggt ctaacagttt ttgaaacttc aaatgctgga taaattcttt gattgggtaa
    25741 aggtcggacg cttcaatgtt tgttccattg atgtagtcgg taatatattc tcgtaacttt
    25801 tctaattcat attccagcca ttcttcctcg ctgtcaaaga agttgtcata atgtccatgt
    25861 tttaattctg catttagacg ttcttctgtt cttaaaaact gacagacttc tttttcggac
    25921 tgattgacat atttccattc ccccgacaac aattcattcg gcgtaacgtc cagcacttcc
    25981 attatctttt ccagcgtttc ataagcagga tagttcaacc ctctttcaat cttggaaagg
    26041 ctctgcatat tgataccaat tttgtccgca agttcctgtt gtttcattcc cctaaatttt
    26101 cttatggtct gtatattatt tcctaaatgg cttattttca ttgcataacc ctccataact
    26161 cgttatatct tcgctgctat aagtctatca tgcttaatat gatttgtaaa gataaaaaaa
    26221 gatattgaca aataagcata taagaattac tatgttcttg taatcaaata agcacaatag
    26281 aatgaaatac aaaaacattc aaaagaaaat atctatctga ttaaacccgt agatatgaat
    26341 atcggttgaa atggaacttg tttgaaagga caaacacagg acttgccgac agagcattgt
    26401 aggactgttg gcgtgcgtca gcaacaacgc catgacggct tgccgtcaaa ggacggaaat
    26461 gctttgtcgg ctggcgaact tgtgagcctt gtctgtgccc ccgttatcta acaggggggc
    26521 actatttcac aaaagacaag tcaaaaacca tacaacccca aagctgtaag gagtaatgag
    26581 gagttttaag aagatagtaa cttcacattc ttttgaatag tggtttacag gtatttcaag
    26641 gctgttgtgg gattggagga atacaatgaa caatttagaa tttgcacttg aaatgaaaaa
    26701 caaacgtctt gcaagcggtc tttcacaaag agaacttgca ggtcttaccc atgtatcaag
    26761 atatagtatc aaccgctttg agaacggaaa agccaacgcc agcaaggaaa cacaaaatat
    26821 tatcctgcgt tgtctgaatt actatatctg cgataagcct ttttatctgc tcgttgatta
    26881 tctgtctgtc cgtttaccaa cgactgacgc attagaagtt atccgaaagg tgcttggcat
    26941 gaaagcagat tattttatcc attatgacta tgggtattat ggctataagg agcattacgc
    27001 ctacggggaa atcaaggtca tggcttctga tgatgaacac atgggcgtat ttttggaatt
    27061 aaaagggaca ggctcacgca acatggaata tgtcttacag gcacagaaca gggattggta
    27121 ttcattttta aaccgctgtc ttgactgtgg cggtgttatc cgacgttttg atttggcaat
    27181 caatgatatg tgcggtctgc tggatattcc cgttttgtct gaaaaatata aaaatggcgg
    27241 tgcggaatgt cgctgtaaaa actatgaaaa tgtacaaagt ggaaaattaa gtgggaaaaa
    27301 ccgcaattta gcaagtaccc tttatatcgg ttcaaaggca agcacaaaat atttctgcct
    27361 gtatgaaaaa caaaaggaac aggcaacgaa aaagaaacat accgatatta tcaaccgttt
    27421 tgaaattcgt ctgcgtgata aaaaggcagt acaggcagtt gaggaattgt tattgacata
    27481 taaccctcat gggctggtgt tttacctcat tactgatttt gtgcaatttc ccgactatcc
    27541 gttgtgggag atattcattt cccatgacag tttacctttt gaaatgaacc ccgtacctgt
    27601 caatatggaa cgcactctgc aatggctgga aagacaggtc atgccgtcaa tcgtgatgat
    27661 agaggaaatc gacaggctga caggctcaaa ctacatgaaa atgattgatg aatgtaccca
    27721 cctttccgaa aaacaggaaa tgcttgtaga acagatgtgt acggatatag cagatgtaat
    27781 cgaaagtgag ggggtgtttt atgagtaatg cacaggatat tcccgtatgg gaaaaatata
    27841 cccttaccat tgaagaagcg tcaaagtatt tccgtatcgg agaaaacaag ttaagacgat
    27901 tggcagagga aaacaaggac gctggctggc tcattatgaa tggcaaccgc atacagatta
    27961 aacgccgaca gtttgaacag gttattgata aattggacgc aatctaatgc aaatgagcct
    28021 tgtatgtgtt atgataagca caagtcatat caaggctctt tccaacaagg aaaggagcag
    28081 acaccatgag agaaaaaaga cgggataaca aaggtcgtat cctgcatact ggagagagcc
    28141 aacgaacaga cggaaaatac ttatataaat atgtagacgc atttggaaac acaaaatatg
    28201 tgtatgcttg gagattgaca cccacagacc cgacaccaaa gggaaaacgg aaaaaaccct
    28261 cacttcgaga actggaacag cagataagac gggatattga ggacggtatc gacagcgcag
    28321 gcaagaaaat gacgctttgc caactctatg ccaaacagaa cgcacaaagg gcaaatgtga
    28381 agaaaagcac acaaaaacaa cgggaacaac tcatgcggtt attgaaagag gacaaattag
    28441 gtgctaggag cattgataca atcaagccct ctgacgctaa agaatgggcg ttacgcatga
    28501 aagacaaggg cttttcctac aataccatta acaaccataa acgctcgtta aaagcgtcat
    28561 tctatatcgc catacaagac gattgcgtaa ggaaaaatcc ctttgatttc aaattaagtg
    28621 aagtcctaga aaatgatacc aaagagaaag tcgcattgac agaggaacag gaacaagcct
    28681 tactctcatt catcaagacg gacaatgtgt atcacaaata ttatgatgat atgctgatat
    28741 tgttaaaaac aggacttcgt atctcggaac tgtgcggact gacagtagcc gatattgatt
    28801 tcaagaatga agttgtgatt atcgaccacc agttattaaa gaacaaggaa cagggctatt
    28861 atattgaaac gcctaaaaca aagagcggaa taaggcaagt gccattaagc agagaaacga
    28921 tacaggcatt tcaacgggtt atgaaaaaac gcccaaaggc agaaccattt gtgatagacg
    28981 gacggagcaa ttttctgttt gtcaatcata agggtaggcc caaagttgcg attgattaca
    29041 acgccttatt tgtccgaatg gtaaagaaat acaacaagca ccacaaggac aatcccttgc
    29101 cacatatcac accgcatacg ctacgccata cattctgcac aaggctggca agcaggaaca
    29161 tgaacccgaa agatttacag tatatcatgg gacattcaaa catcagtatc acaatgaact
    29221 ggtatgctca tgcgtccata gataccgcaa aatcagaggt tcagcgtcta atcgcataag
    29281 aagtatttac cacgatttta accacgattg acagggaaaa tataagaaga tagatctaga
    29341 tatgtgaggt ttaccacaaa aacaaaatgc ccgtagagcc tataaaataa ggctttgcgg
    29401 acatttaaga agatataaaa agatagtcaa aaagacatat ataatttatt ctaaaaaaac
    29461 aacaattttt cgttctcatt tcttctttta tgttacactt gcgctagcat ggttcaacag
    29521 atgaaagcaa acaataacac ggttttttag acccttgtac aaagattcct acataacaaa
    29581 atgaggtgga gaaaatgaaa gatttaaggt atagaagtgt ctttgatatt attggacctg
    29641 tgatgattgg tccaagtagc tctcacactg ccggagctgc tagaatcggg aaaatcatac
    29701 gtagtatatt tggcgaacag cctgattctg tagatattta tctatatgaa tcatttgcaa
    29761 aaacctatcg ggggcacgga acagatattg cattagttgg cggcctgtta gggatggaac
    29821 ccgatgatga acgtttagct gattctttga aattggcata tgaagccggt atggaagtct
    29881 tgtttgttcc taagagtgaa aaagccgaac atccaaactc tgtaaagatg cttctaaaaa
    29941 aaggaacacg aaagctttca gtcactggca tttcgatcgg cggtggcaat atccagattt
    30001 ctgaattgaa tggattcaaa atttctttaa gcatgggtac tcctaccttg atcgttgtcc
    30061 accaagatgt tccaggtatg attgctaaag tgactagtat actttctgag accaatacga
    30121 atatcggcac tatgacagtg acacgggaat caaaaggtga aaatgccatt atgataattg
    30181 aagtagatga tcctcaagta gaagaaacgg taaaaagact caaacagctg ccaaatatcg
    30241 acagcgtaaa ttatttcgat taaggagcta aaaaaatgtt tttatcgatt gaagaattag
    30301 tagatcaagc aaaagcttat ccaagtgtag cagaattgat gattgcagtt gaaatggaaa
    30361 tgagtggccg ttcccgtagt cacatcatcg agatcatgga aaaaaacctc tcagtaatgg
    30421 aacaatcaat caacgaggga aaagaaggtg ttacctccgt cacaggaatt accggtggtg
    30481 atgcaaaaaa attagatgat tatttaaatg aggcagactt tttaagtgga gaaacaatct
    30541 taaaagctgt acgcaatgct gtagctgtta atgaagtcaa tgcaaaaatg gggttgattt
    30601 gtgctacacc gactgccgga agcgcgggtg ttgttcctgg tgtgctgatg gctgtccgag
    30661 atcggcttga tttgacacat gaacagcaat tggaattctt atttaccgct ggcgctttcg
    30721 ggttagtcat cgcaaacaac gcttctatca gtggtgcaga agggggatgt caagcagaag
    30781 ttggttctgc aagtgcaatg gcagcagcag ctttggtttg tgcaaaaaat gggactcctc
    30841 atcaagcagc acaagcagta gccattacat tgaaaaatat gatgggactg atttgcgatc
    30901 ccgttgcagg cttagttgaa gtcccgtgtg tcaaacgtaa tgctttaggc tcttctcaag
    30961 cttttatctc tgctgatatg gcattagcgg gtatcgaaag tgtcattccg ccggatgaag
    31021 tcgttgctgc aatgtaccaa gtcggacgtc aaatgccgtc gattttcaaa gaaacagcag
    31081 aaggtggctt agctgttacg ccaactgcta aacgacttgc ggaagagatt ttaaataaga
    31141 gggcgtgata aagctcctta atttcgagta ttaagagatt agttatcttc ataacaaaaa
    31201 acgaagagag gactaattgc ccttctcttc gttttttatt aatccatcaa tttcgtattt
    31261 tttacttcat cgatcgtttt tgttcctgca agttgcatcg tgatcgacaa ttctttgttc
    31321 aggtgttcaa aaacagatgt tactccttct gccccaccta gattcaaccc ataaatcact
    31381 ggtcggccaa tcgcaactaa gtcagcacca cttgccagag ctttgaagac atgctctcct
    31441 cgacggacac cagagtcgaa aatcactggt acacgtttgt tgactacttc ggcaattttt
    31501 ggcaatactt caaaggacgc aggacctcca tctagttgac gtccaccatg attggatacc
    31561 cagataccat ctgcccctgc agagatagcg attactgcat cttctggcga ttgtattcct
    31621 tttacgataa ctggaagatg ggtgatttct ttgattgttt tgatgtcatc tggtgtcaag
    31681 ccttgttttg cagctgcata aatttctgcg attccttttc cttcgccatt accacttgca
    31741 ctttgttcgc tatacgcagc taagttaggc attggtaatg gaaattggaa ttgattgatg
    31801 acgtcttctt cacggtaacc accgagcgtt gaatctgccg ttaaaatgat agctttagcc
    31861 cctgcttcta ctgctttgtt caaaatgaac tcattgaatc tgtcgtcttt actcatatat
    31921 aattggaaaa actgcggtgc gcctggcgct gcatcggctg cgtctttgat tgtcgtattt
    31981 gcataagtgc tgatggaaaa gattgatcca gcagcagcta ctcctttagc tgtatcagct
    32041 tccccttttt catgtgccaa tccttgggcc gctgatggcg cttggataat cggtgtatct
    32101 aattcaattc cgaaaacaga tgtatgcaaa tcggctgagt cgattccgcg taatatccgt
    32161 ggcatgattt tctttgtatt aaaagaagtc gtattttctt tcatcgtcca ctcatcttca
    32221 gaaccaccgc gaatatagcc aaaagctcct gcttccatgc gctcttttac acgaccttct
    32281 agttaggcaa tgtttacaat atcgattgga tgttcttcat tacttgtatg ataagtcata
    32341 gtagatcctt ctttcatctt cttgattaca tataaatcat actaaactta caaaaagtaa
    32401 gcaaacattt actctcagat acttttatcg ctattctttc caaataaatc gacttacaag
    32461 ctgatctact tcttggtttt catgaagcat actatgagag gcattttcac ctgtcactac
    32521 tttttcctct atttcatttc cgcgttgctt caacaacgca tagaccgcta atgcactgtt
    32581 taacggtact gttccatcac tcaagtctgt ttcatctagt tgaccagcaa tcaagaaaaa
    32641 tcgagtggtt ataggaatat taccgatcat ttgctggtaa tcttgatagc ggttagaaac
    32701 gacagcaggc ccctttgcta actcatctgt cagagactgc gcactatcat cgacaaaatc
    32761 attaaaaggc gcgccaattg ctgttaatgt ctgaacttgt ggcaatgacg tatcctgtcc
    32821 atacgttcct aaatagcgta gtgcactaac tcctcccatg gaatgcccca ctatgttgac
    32881 tttatctatg ccgtagtttt ctttcagata caaaagacaa gttttgatcc attctgcttg
    32941 attccactcg ttatttttat tatcttcaaa caatacttgg accatcggat tgtctgcttt
    33001 tccagacaaa tctcctgttg cttgtatttg tccatctgtt cctactgtta aaaccaattc
    33061 ttttactgct tgctgatttt tttccaaacg ccgcaacatc cctttaaagg aattttctgt
    33121 cccgctgtag ccgtggaaaa aaagtgttgg aatagtggtt ttttctatca ctttactgga
    33181 ttctgccgtt ttttctttat cagaaacttc agtagtattt atcttcgtcg tctggcaacc
    33241 acttaacgca aacaaactga taaggactaa agctgctgca gttgcacgtt tgcataaatt
    33301 tttgattggt tctgtcatac acctcttcct ttccatatag aaaaaagaaa gacttgaaag
    33361 gccaatcata tgatcgacat ttcaagtctc tttattaatc gattttatct acttgatcga
    33421 aatcaagttc tgtactggtt tcacgtccaa acatatcgat gttgactttc aatttttctt
    33481 tttctccgtc aacttctgtt acttgtcctt ctaatccagc aaatgcgcct tcgatgattt
    33541 tcacgacttc gccaacttct acttctagat cattttgtcg caggctcata ccgatagaac
    33601 gcaagatatg attgacttct tctggaagta atggtgctgg tttgcttccg gctccgtgtg
    33661 aaccaacgaa tcctgtcaca ccaggtgtgt tgcggacaac ataccatgaa gcgtctgtca
    33721 tgatcatttc aacaagtacg tatcctggga atgttttatg aacgatttct ttttctttcc
    33781 catttttgac ttcagtttct ttttcttcag gaacaacgac acggaaaata aagtcggcca
    33841 ttttcatact ttgcgcacga gattcgatat ttgcttttac tttgttttca tatcctgaat
    33901 atgtatgcaa gacataccag tttttttcaa aagattccat tgaaggctcc tcatttcatt
    33961 gtaaaataaa aaaacctcag tacacccgaa gtttttcttt cgcttcatta tagcactaaa
    34021 agtacgttgt ggctagcgag ttattttaaa atccagccaa ataagctttg gatggctgta
    34081 tccattacgt aaaacatcaa tccgaaaata atagaagttt cgatgactac taacgtatct
    34141 ttacggagtt gttttctgct tggccaagtt acttgtttga tttcatcttt tacgcttttt
    34201 agaaatttca tgagacaacc tcccttgcta ctgtttattt cgtttcttta tgtaatgtat
    34261 attgattaca atatttacaa aacttattga tttccaaacg ttcgccgctt tttccttcac
    34321 tgactgattt tgtgtagttg cgagacccac aaacggaaca agcgagtgct gcttttttct
    34381 ttgccattta cttctgcccc cttgctgaga atcagactta tacttcataa cagtatcacg
    34441 taacttttta cttgtcaatt tgtgaaataa actatctatt gttttggata aaattatgat
    34501 atgattaatt tgaaattaag ctaaaatgag acaattggag gtatagcgat gctattaaaa
    34561 acacttgtat ataagaaaaa agatctgaca accgttacag agactagtac attagaagaa
    34621 gcattaaaaa ttttagaaac ttccggatac cgttgtgtgc cgatcttaga ttcttcaggt
    34681 aatattttcc gtggaaacat ttacaaaatg catatctatc gtcataaagc aaatggcggt
    34741 gatatgacgc ttcctgtcac tcatcttttg aaaaacgcta caaagttcat ttacctggat
    34801 acttctttct tcaaagtatt ctttacgatc aaagagcttc cttatattgc tgtccttgac
    34861 agtgaaaatc atttttatgg tattttgaca catagtacgt tgcttaatat gctctctcaa
    34921 tcttggagcg tagaacaagg aagctatgta ctgacaattg cttccacagg aaagcaagga
    34981 gatttggctg ctattgcaaa aatcatttca aaacacagca gcattgccag ctgtattact
    35041 cttgatatcg aaaaagacga atacattcga cgtacattga tcacgctacc ggctaacact
    35101 gaagcgaaag tatgtgaaac gatcgtcaac cacttagaga aaaagaatta taaagtggta
    35161 gaaattgaaa atttgcaaaa ccaagaagca taatcaaaaa gcgaaaaaag aaaacagcta
    35221 atctgctttc ttttttcgct ttttataatt agacacgctt ttcaaaatca agcgcgcgat
    35281 ctaaataata catcaatggt gctgaaacag ccatgacgat aaactcactt aatgcaagcg
    35341 tgccataggt tgtccaaaat gcttggccac ctgttggaac taacatataa gcaatcagtc
    35401 ccatgctgat ggtaaagaag atcgtgttca atactaaacg catctttaca tttttcactt
    35461 tgttttgcaa taaagctgtc aggcctaaag aaagcaatgt ttggccgcca ccaaaaacca
    35521 catccatgat accaaaacca aagaaggcat tatagacgat gaccccgcct aaaactcccc
    35581 aaagcaattt acggttaaag acaactaaat gattcaaact ttctgaaata cgaaactgga
    35641 tcggccctga ggatacaggc gcaactaaaa tagttaacgc tagataaaga gccatgatga
    35701 tgccatttac aacgattacg gataaccgtt gtctgctttt cgttttttgc attttctctc
    35761 ctcttttctc ctagtttttt taacgcggga tggttgatga accgcggtta tgtgaaacaa
    35821 acaataatta taacagactt ttcatttatt tcaatccgtc ttatctgctt ttaaaaaagc
    35881 aaaagcttaa acaatgtaaa cgtttgctaa aaatcgtggg atatggtata aagtaagtgt
    35941 aaggggtcat aagaattccc agtaaagcag acgaagtagg atctgcaaga acagaattgt
    36001 tgtgaaactt aaataaataa aaaaggatgt gggaatttga gcaacctctt actagagaac
    36061 ggtctagcaa gcagctgaag atctaagtgg acatgccgaa ccttggaact tctccttacg
    36121 taagtaatgg atcatgcgct tgctaggctt ttttgtgttt ttcggtcttc ggaccttttt
    36181 cattttccta gacttctgct ataataacca agaattaatt ttgtaacaga tttttttgaa
    36241 aaaacaagtt tctttcattc aaaaaataga aaaagcttgc taattattgg ttaaccagca
    36301 aacaggagta ttaaaatatc ttgttttatt gaatagcagg aggaaatcat gaaattccat
    36361 tatcatatat tgatcaatcc atcggcaggc agcggaaatg gctataaagt agcagaaaga
    36421 attttaccgg tattaaaaaa taagcatatt gactatacac ttcattatag tgaatacaaa
    36481 ggacacgatg ctgagattgc tgaaactttg gcaaaagaaa cattgattcc ttggatagaa
    36541 gaagaacaga aaacaatcga tactttcccc cttttgatca tcgtaggagg tgacggcacc
    36601 ttacatcagg tacttgatac tttctatcag atggaagtag aattcccagt tgcttatatt
    36661 ccagcaggtt ctggagatga ttttgctcga ggggcaggcc ttccaaaaaa tcccaaaaaa
    36721 ggacttcagc tgattttagc agctcaatca ccagaaaaag tccacataat ggcttatgaa
    36781 gaaaaaataa gtgaaaaaaa aggaatagct gttaataatt tcggtatagg attagatgca
    36841 gctatcgtcc atgcgacaaa tcattcttcc acaaaaaagc gattgaacaa atacaactta
    36901 ggatcatttt cgtatttatt ctctattctc cgtgctttat tcactcaaaa aggatttcca
    36961 attctggtag atgcaggagg aaaacaatta agtttttcaa acgcattttt atgtacagca
    37021 acgaagcatc ctttttttgg cggaggaatc gcgattgttc caactgctga tgcgtctaaa
    37081 ccagtcattg attttgtcgt ggtcgaacga atcaatttct ttaaaatctt gtggctcatt
    37141 cttttactta tacagaaaaa acaattgaaa tcgaaatatt tccatcacta cacaacaaac
    37201 agattacgta tcgtttcaac aacaccgcaa catggtcaag aagatggaga agaaatggaa
    37261 aaacgacctt ttgatatcac aataaccaca actaaacagt tgttttggtg ttaataaaag
    37321 aggttttgtc agaagctaca tctggaacac cgtttattcg gaaaaaagga ctgtgacacg
    37381 acttttgtca cagtcctttt tttactatgc agttaattct ttgcccaatt gggttgccat
    37441 cgatagtttc tttctttacc caaaataaat tggatcattg aaaacaccgc agcaaaagca
    37501 actaagacac aaccattgaa ccaaaaagat tctgcaaatg tttgattgta actgagtgtt
    37561 tcactgatca agttattatt ttgaacaaac caatatgttg gtgtgaatgc cgctattttg
    37621 ctaaccattt ctggcaagta ttctgctggt acaaacaccc caccgataaa gcaactgcct
    37681 gtgataaaaa tatctttgat cccaccgctg gtttctgagt tttttaccaa tgaagtgata
    37741 caagccgaaa agctaatgat cggcaataaa aacaagaaag tatttaaaat atgataccat
    37801 accgttatat cccacgaggt gtggatcact aacagaacca ttagaagtaa gaagccactg
    37861 aacaacagca gcgagtaact aatcaaacta aaggttactt tgcgattcaa gtttcgtttg
    37921 gtaatgggcg cactctggtt tcgtttactg atctctttgc gattaaatgc tagattgatg
    37981 acgctgaaac cgctaaaaat ggatagaaac aacccataag acaagaaatt aaaggttgtg
    38041 cccgtcaaca gctttttcat ccgctgacta tatcccgcac ttagctcaac ttctccagta
    38101 accgagagtg ccatttgtgt ctgttccata gctgcatcga ttgttttatc ttcttcttga
    38161 aacagaatca gtgtttgcaa aaaggtgttc acttgctgtg taacaactgt tttagaaaag
    38221 gcatctgggc gactctgtat cttgatcgtc ggcatctttc ctacttcgac ttgttcggta
    38281 aagtctgcag gcagatgtaa gataatgttt acacgattaa aataaagagc atcatcgatt
    38341 tctttttgtg aggttccatc caatgtaacg actgtttcct tgtcattcaa atatttacga
    38401 aggccatcga ctacaggcga ctcttcttca ctaatcaagg cgattttcgc atcttgcaga
    38461 gaggtatttt caacatcaat attttgcgca aaaaagaatg tggcaacaac agtgataatt
    38521 acacctatca gtaaattcga tttatttgct ttaagcaatg tccaaatcgt tttataaagc
    38581 gtcataacgc acccctcttt ccatccaagc atttcctaac gcaaaaagga aaataaatcc
    38641 gatcaaccat agaagattgg tataaaaact agccaaatca gtgtagaaat aaagttt
//
LOCUS       DAJSBN010000025        38094 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJSBN010000025 DAJSBN010000000
VERSION     DAJSBN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.025"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(504..1448)
                     /locus_tag="ONT86_001412"
     CDS             complement(504..1448)
                     /locus_tag="ONT86_001412"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR2352216.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1744..2688)
                     /locus_tag="ONT86_001413"
     CDS             complement(1744..2688)
                     /locus_tag="ONT86_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2352217.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2909..4123
                     /locus_tag="ONT86_001414"
     CDS             2909..4123
                     /locus_tag="ONT86_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2352218.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4145..6160
                     /locus_tag="ONT86_001415"
     CDS             4145..6160
                     /locus_tag="ONT86_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HCR2352219.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6243..7616)
                     /locus_tag="ONT86_001416"
     CDS             complement(6243..7616)
                     /locus_tag="ONT86_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR2352220.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7642..7926)
                     /locus_tag="ONT86_001417"
     CDS             complement(7642..7926)
                     /locus_tag="ONT86_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2352221.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7928..9943)
                     /locus_tag="ONT86_001418"
     CDS             complement(7928..9943)
                     /locus_tag="ONT86_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2352222.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10122..10427)
                     /locus_tag="ONT86_001419"
     CDS             complement(10122..10427)
                     /locus_tag="ONT86_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCR2352223.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10516..11547)
                     /locus_tag="ONT86_001420"
     CDS             complement(10516..11547)
                     /locus_tag="ONT86_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2352224.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11754..12629
                     /locus_tag="ONT86_001421"
     CDS             11754..12629
                     /locus_tag="ONT86_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2352225.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12781..13623)
                     /locus_tag="ONT86_001422"
     CDS             complement(12781..13623)
                     /locus_tag="ONT86_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2352226.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13715..15592)
                     /locus_tag="ONT86_001423"
     CDS             complement(13715..15592)
                     /locus_tag="ONT86_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2352227.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15589..17010)
                     /locus_tag="ONT86_001424"
     CDS             complement(15589..17010)
                     /locus_tag="ONT86_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2352228.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(17013..20402)
                     /locus_tag="ONT86_001425"
     CDS             complement(17013..20402)
                     /locus_tag="ONT86_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR2352229.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20659..22050
                     /locus_tag="ONT86_001426"
     CDS             20659..22050
                     /locus_tag="ONT86_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2352230.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22123..22770)
                     /locus_tag="ONT86_001427"
     CDS             complement(22123..22770)
                     /locus_tag="ONT86_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR2352231.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22778..23464)
                     /locus_tag="ONT86_001428"
     CDS             complement(22778..23464)
                     /locus_tag="ONT86_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2352232.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23470..24543)
                     /locus_tag="ONT86_001429"
     CDS             complement(23470..24543)
                     /locus_tag="ONT86_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2352233.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24765..25439
                     /locus_tag="ONT86_001430"
     CDS             24765..25439
                     /locus_tag="ONT86_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2352234.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25444..26784
                     /locus_tag="ONT86_001431"
     CDS             25444..26784
                     /locus_tag="ONT86_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2352235.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26933..27919)
                     /locus_tag="ONT86_001432"
     CDS             complement(26933..27919)
                     /locus_tag="ONT86_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCR2352236.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28643..29674
                     /locus_tag="ONT86_001433"
     CDS             28643..29674
                     /locus_tag="ONT86_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2352237.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29783..31315
                     /locus_tag="ONT86_001434"
     CDS             29783..31315
                     /locus_tag="ONT86_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCR2352238.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            31454..32266
                     /locus_tag="ONT86_001435"
     CDS             31454..32266
                     /locus_tag="ONT86_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCR2352239.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            32256..32741
                     /locus_tag="ONT86_001436"
     CDS             32256..32741
                     /locus_tag="ONT86_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2352240.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            32764..33309
                     /locus_tag="ONT86_001437"
     CDS             32764..33309
                     /locus_tag="ONT86_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCR2352241.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33382..35025)
                     /locus_tag="ONT86_001438"
     CDS             complement(33382..35025)
                     /locus_tag="ONT86_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR2352242.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(35154..37382)
                     /locus_tag="ONT86_001439"
     CDS             complement(35154..37382)
                     /locus_tag="ONT86_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2352243.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(37415..37894)
                     /locus_tag="ONT86_001440"
     CDS             complement(37415..37894)
                     /locus_tag="ONT86_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352244.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        11117 a         7202 c         6927 g        12848 t
ORIGIN      
        1 ggaaatagtg gtttagaact agtgggctga ttttttatat attttagttt acataatata
       61 tattatacaa agtagattta aaaaatgata aacctctctc gaacggtttt ttatcaaaaa
      121 tcgtcatttt tccatcaaaa tctgcttatg caaaattata aaaaaatttt gcagtttttc
      181 ttactttttt tgtcgtttaa ttgaatgata aaaaccagtt tctttattca gttaatcaaa
      241 tatgaacgaa ttagatatga atcattgtat ttgccgttgc ggaaggattt tttaattaac
      301 cacagataaa cattgataac gattcgctta ttgtaatcaa cagattaaat ttcatatgtt
      361 catatgtacg gatacatttt cggaccgatt aattttaacc attctgttta tttgcttacc
      421 tattagatat caataaaaaa ctgcttaaca gctatttcag ctgttaagca gttttaggat
      481 ttttagttca tattcattgt ttcttataat acagtgttca atacttctct taaagcactt
      541 gttgatttgc tgaacattgc tttttcttct tcgttgattg ataattcaac aacttcacga
      601 acaccatttt cgtcaacgat tgatggcaca cctgtaaaga tatctttttc gtcgtattca
      661 ccagttaaat aagcagatac aggcaataca gcttgttcgt tattcaagat agcttttacg
      721 atacgtgcag tactcatacc aataccatag taagttgctt ttttacgatc aatgatctca
      781 taagcagcat tacggacttt atcagcaatc acatctaatt catcttgggc aatacgatgg
      841 tctttttcta caatttcaaa cactggttta ccaccgacag ttgtatgtga ccatgcagca
      901 acttctgaat caccatgttc acctaagata tagccgtgga cgctgcgtgg gtcaactttt
      961 aatttcaatg cgatttcttt acggaaacga gttgtatcca atgttgttcc agtaccgata
     1021 acacgtgatg ttggcagacc agattcgttc caagcgatat atgtcaaaat atctactggg
     1081 tttgaagcaa caacgataat accgtcgaat cctgatccca tgatttcttt tacgatttgg
     1141 cgcatgattg acgcatttgt tttaactaaa tctaaacgag attgaccagg tttttggttg
     1201 ataccagctg tgataacaac gatatcagca tctttacatt cttcatagcc acctgaccat
     1261 acagccacat tttcatcgcc ccaagccata ccatctaaaa ggtccaatgc ttccccttct
     1321 gctttttctt gattcacatc gatcaatact aattcattag agattccttg gttgatcatt
     1381 gcataagcga tacttgttcc aacaaaacct gtaccgacga ttactacttt acgacttgtt
     1441 tttttcatga ataaacagct ccttttcata actatttgtt actttcatga aaagtatagt
     1501 cttgtttatt cattttatca acatgttaat tataatagtt gttattataa tttttgatgg
     1561 aaacgaattc ctgaatacgc ataacagata aatgacaaat gtttacacct atatcccctg
     1621 ataatacaac caaaagttta gaactgaaaa taaatccaaa aaaattcgct gcaacgatat
     1681 aataagacag caatcgcacc taaaacaatt ttccttcata tgctttagtg aaagattttc
     1741 ttcttaatgt ttatttctcc attcattggg agtacatgtt gaatacttct tgaataattt
     1801 gaataaagaa gtcgtttgtt tatatccgat acgattagcg atttcttcca acgaaaaact
     1861 cgtttcttga atcagttcca acgcaaagtc gaattttttc tttagaacca gttcttgaaa
     1921 ggaagcatca aaatacattt tcaatttatt actaagatag accggattgt agccaaaatg
     1981 agctgctgtt tcttttaacg tacaagaagt aaaatgctta tcgatataat ctaatactct
     2041 aagcatctca ttttctgcgc atccatctgc tgatgaaaag ctttgttgtt ctaataaatg
     2101 agtcaattcg atcagtaata gtgatgtata gagatttaac gaacggatct tatgactgta
     2161 tgtatcaaaa taaatcaaca gcatattttc aatcaaatgc gtcaaacgac ttgtttttcc
     2221 gccgcgaaaa tgaatgaatt gtgtatgttc ccctttttct tttgatgcat ttatcaaaaa
     2281 agaagaaagt agattatttg tgcttgccaa atgattcaat atctctgtag ataaggtttc
     2341 ttcttttaat aagatgttga ttaaaatatc ctcttgccct gtgtattcga ttctttgagt
     2401 agcttcttta tcaatgatca ccacgtcact ttcttccatc tctatccttt ctccattaat
     2461 aatttgtaaa catgtaccgc tatacatata attgatttca acaaaatcat gtgtatgctc
     2521 gggaacttcc gcatatcgac ggtgtttact gatatgaata tttccctgtt gaaatagatc
     2581 ccctcggtgg atttttggcg gtgtctgaag atggtaagct aagtttaaat cattgatatt
     2641 cattccttgt tcacgctgca gagtttcgat tgattcttcg ttacgcaaaa aaaatgtcta
     2701 attcagctag ttccaattga ctccctcctt tttgtaagcg gtttctttcg cttattagtt
     2761 cacgctgtac tgctattata tgcaaagcta tttttggttt cataaagact ttctatggtt
     2821 gggacatgaa cttcctgtga ttccgctatt gagagaataa ccacttcata ctatattttt
     2881 gataagcgct tacaaaaatg aaaggaacat gcttatgaat aaaaagatga tgaaattttc
     2941 tctgctgtca gtctctctta ttttgacttc tgctggtgca gtcacgtctg caattcccag
     3001 tatgtcagct acttatgcca cgcgttcttt atctgctgtt gaattattaa cgactgctcc
     3061 ctcttttatg gtcactattt ttgttttgat tagtagtttt attgctagga aaatcggaca
     3121 aaaacaaact gtgatcatcg gattagtgat tgcatcgatt tgtggtatcc tccccctctt
     3181 ctcacaaagt tacagtattg tcttagcttc aagagctgga ctaggtattg gctttggatt
     3241 gatcaattct ttggcagtca gcatgattgc tgccttttat cacggagatg aacgagcctc
     3301 tatgattggt tttcaaagtg catttcaagg atttggtgca gctttaatga catttgttgc
     3361 aggacagcta gtacagttcg gttggcaata taccttttta gtctacgcca tcagcttacc
     3421 tattcttgtc ttgtttgttt tgtttgttcc tacacctgaa acaactgaac ttcaatcgca
     3481 tggcacagcc ccaacaagac aagtaatcaa aacgaatcct aaaatgatta gttatggtat
     3541 ttttctcttg ctggtcatcg tggtatataa tgctgtatcg attaaattag ccacagtcct
     3601 gacgacttat tctctaggaa acgaaactaa tgcagcgaat atcttaacaa tcatgcaatt
     3661 ggcaagtatg gctgccggag tttgttttgg ctggcttcag acaaaagcac atcgctatat
     3721 gcttacactt tctttactac tgatgggaat cggctttgta ttgattgcca ctgtgataaa
     3781 cctgtatgtg attgggtttg gtgcgatcct aacaggaatt tctttttcgt tattcattcc
     3841 ttacttattc aaccaagtaa gtatccaagc gcccaagtac gcggagagtt tcaatacttc
     3901 aatcttattg gttggcgcta atttaggtac tttcattgca ccatatggtt tgctctttct
     3961 ctcttttgct tctcttggga gtaaaacact tgctgttttc gtcaatggca gtatcttgct
     4021 gttgattagc gcgcttatta gccttctagt agtacacagg acgaatagaa agacagtgat
     4081 aagagtagat gaagaaataa aagaacaatt gacaacaaaa taaatttgat ggaggtcgtg
     4141 taaaatgaca aatgaaaaac ataaagatag atggctttgg tatcctggag actttgagat
     4201 ccgccatgga ctgctacaaa atttccaacg ggaagaacga ggttttgact ggccagcgta
     4261 ctggtacatg gatgattgcc atcggaacgt caagtttaag agatattact ttcttgatca
     4321 accttccatg tttaaagtga cgatccaagg agttgggtat gtagaaatta atggacaaaa
     4381 acatccatgt ggaaaatggc tgacttgtcc agctggtaaa gcaaaaattc gtatctttgt
     4441 aggacataca tcagggctac cagcgatgtt tatcgaggga gacgaagtaa aatcagatat
     4501 tggctggaca gcaagtaatt ttattgaaga atatccagct ggctggagtc cgctttatgt
     4561 cgatatcgca aaagatccaa atcaaatcta ttatcaaaaa gaatgcgtcc atccagtcac
     4621 tgaaacggag attggcggag gaatcttatt cgattttggg cgagcagtga atggctgtgt
     4681 agaactaata tctctggatg accatatcga atctatcact ctatgttatg gagagtccga
     4741 tgtagaagct atggatatcg attattgcta ctataagcaa ataggaatga caagaaaaat
     4801 gaaagctcgt aaacgcgctt ttcgctattt gtttatacca gaggtttcta ctggaatggt
     4861 tcaacttcaa gtttttcatg aatttttatc tctttcgaaa caagctgctt tttcaagcga
     4921 ttcttcttta ctgaatcaga tctggtcagt atcagaagaa gccttcaagt tatgtagtga
     4981 tttgtttttt atcgacggta tcaaacgaga tcgctggatt tggtctggag atgcgtatca
     5041 atcattcatg atcaatcaat atcttttttt tgacgaggaa atcaataaac gaacgatatt
     5101 agcattaaga ggacaaaatg aaatcaagca gcatatgaat actatcgttg actattcgat
     5161 cttgtggttg attggtatcg aaaaccacta tatgatgacg aaagactatg aatttttacg
     5221 catcgtctac cctaagatgc aatcattaat gagatacttg atggagcaaa cgaatgaatt
     5281 agggtttatt tatgggagag caaaagattg gatatttatt gattggtctg aaatggataa
     5341 agaaggaacg cttgctgcag aacaagtctt attactgaaa gcctatcatt ccatgatcac
     5401 ttgcggagat atcttagctg aaacatatgg agagaacgaa aattatcata ctcatctcta
     5461 tcaagaaaaa tatcaactat tgaaacaaaa cctatttgac tacttttggg atgaagaact
     5521 tggcgctttt atcgattgct atgaatctgg gagaagaaat gtgacgcgtc atgcgaatat
     5581 tttcgctgtt ttattcgatt ttgctaacaa agaccaacag caatcgatct taaagaatgt
     5641 cttattgaat gacactatca cacagattac tactccatat ttcaaattct atgaacaaga
     5701 tgctttatgc aaactcggac agacgaatat cgtctaccaa acgattcttg attattgggg
     5761 agggatgtta tctcatgatg cggtaacttt ctgggaagaa tacgacccta ctcaaacagg
     5821 aaatgaaaag tacagtatgt atggagatcc ttatggaaaa agcttatgcc atgcatgggg
     5881 agcaagccca atctatcttt taggacgcta ttttatcggt ttgtatccaa caaagccagg
     5941 ttatgaagaa tttatgatac aacctaatct agattcattt gaaacactga attgtacgct
     6001 acctatcaaa gatggaaaga tttcgcttga aaaaacaaaa gataagatcg tgattacttc
     6061 tgatagaaca ggcggaacag tgaaaatgaa tggtaaaagt tatcctcttc tagcaaaaca
     6121 gccacttgtc ttaacaagct attcaaaaat aaatgcataa aacaaaaaac tgcttgcgac
     6181 atatcagaag actttaaatc ttcttctgat attgtccaag caatttttat tttgaagttt
     6241 gcttaattct gttctttaga aacaatgttt gttctttttc cggcaaaaag aaagctagat
     6301 aatacgacaa gtaaaaagaa agcgatgatt ccaactgtca aggctatttg accgtattct
     6361 cctagtttcc caagtacaag tcctggtact agataatcag tatcggcaaa tgtagaagaa
     6421 gcgattttaa gatctcctaa tactggaagg ataaataatg gcagccaact aatcaataag
     6481 ctattgaaag cagaacctaa taccgcccct cttctaccgc cagtcgagtt gccgaagaca
     6541 ccagaagcag ctccgcagaa gaaatgtcct actaccccag gaatgataac tgtagtttgc
     6601 aagcctatca tagcaaacat ggatagtgtt cctacaaaga agctaacaaa aaagccaatt
     6661 aatacagcat taggtgcata tggaaaaacg ataggaacat ctaatgctgg tttagaatcg
     6721 ggtacgagtt ttttagcaat cccttggaat gctggaacga tttctgccaa caccattcga
     6781 accccttgaa ggacgataac aaacccagct gtgaatgttc cagcttggat cagtgcaaag
     6841 attacagcgt tgtctccatt gcttagttct ctttcaacaa atccaggacc agctaacaaa
     6901 gcaagtgcga catatacgat cgacatcaag agcatgatcg atacagtaga atctcgcaaa
     6961 aagctgaaac ttttaggaat ctttatgtcc tctgtagaac ggtctttgtt tccaaaaaac
     7021 tttccgatcc atccactaga agcataaccg atatttccgg tatgccctaa cgcaacttga
     7081 ttgtttccag tgatgttctt cataaacggt tgacaaagag caggcgtaat agttaatagt
     7141 gttccttcga agaccccgcc taataagatc atccacaaat ttgcagtaaa ccccgcgctt
     7201 actaaaatga ctgctgttag acaagaaaca taaagcattg cttgacctgt aagaaaaatg
     7261 aatttatatc ttgtgaatct agcaagtaaa atattcacga tcatccctac tagcatgatc
     7321 aaagcagtag gtgtaccgta ttgttgcaaa gcaatcgcca ctactgcttc attgctagga
     7381 actactcctt gagtatgcag agctgtttgg aacatcgaag caaaaggggt tagtgaatta
     7441 ctgattaaac cagcaccgcc gatcagtact aggaatccga caaatgtttt gatagttcct
     7501 tggatcactt ctgaaaattt cttcttctga agaacgagac caagcagtgc aatcaaagaa
     7561 acgaggatgg agggctgact gacgattgac acaaacaaat tcaacaattt atccattgat
     7621 agttcctccc ttgaatcatt tttattcttt tgacaagatc ttcgttactt tttcttttac
     7681 ttcttcgctg tcaatgatcg attgcaaagg tacgagcttt tcatcaggta tagaagttag
     7741 cgtatctttc aaagaatgct caatgaagaa ataatcagcc atatcgctag atacacttcc
     7801 aacatccgta tgacttactt caaactcgtc cacatctgct ccaagatctg caagtgcttg
     7861 tttgatgttc atctctacca taaaacttgt tcctaatcca ctttgacata ctgcaaaaat
     7921 tttcatatca ttcgtctcct tttcttatga tcgctaatat ttcttctttt gttgatgcgt
     7981 gtaataaatc ttgaagattt tgtttattag aaagtagctt tgttaaattt gccaaagccc
     8041 gaagatgtgc ctcattatcg atagcagcta aacagatgaa caaactaatg ggatgggctg
     8101 gattatcaag cagatccaca ggttgttgaa gtttcagcaa agacatccct acttcgttta
     8161 cgccatcttc tggacgagca tgaggtaatg cgatgtgatc gccaatatgg ataaacgcac
     8221 catactgctc cacgcgctcg atcatagctt tgatataact ttcttctatt ttattttctg
     8281 ataatagcgg ctgtgcggca aatgtgattg cttcttgcca gttttttggt tgatccgata
     8341 attggatcat ttcttctgtt aataattcag atagcatagg acgttgatcc tccttctttt
     8401 ctaaaagttt gttcatttta cgatttaata cttgatagag cttttcttcg tctacgcctt
     8461 cttttaattc gatataaggc agtagcgccg acaacaattc ttgggcagaa ggcattgaaa
     8521 atcctggtat cagccaatct ctttgtactt gattgatcag ctgatttttt tccaagtcag
     8581 tcatgattgg atgcagaaca tagagagact ttttcttatc agtaagtgtg agaggtacag
     8641 tagaaaaaat aatatcgtaa gaagtttcgt caacctcgtg taagcggtca gctgaattag
     8701 tctctgtaaa aaagatcatc gggaataatt gctgaagttc actttttaag atcaatgaag
     8761 agctaatgcc acttggacag acgatcacgg ctcttatttt tctatttctt tcttctgcgt
     8821 ccgcttttct gatctctccg ccaaatagta tagtgaaaaa accaatctct tcttcaggga
     8881 tgctcttccc tattcgtctt tctaacggaa gtaatgcttt tcttgtcatt tcgtagatgg
     8941 aagcatattg atgcttaatt tgatcgatca tcacattagg taaataaaaa gaataggaaa
     9001 tacgaaaata ggcagggacc aagtgataaa agacattcat caataattca cgaaacgaag
     9061 caaattctac ggccatcagg aattccatgc gatggatgac ttctgaagaa cattccagca
     9121 aaaaatcaag tgcaggctcc tggacatctc cttcaacgat cgatgctaag caaagagaga
     9181 aaaatataat ctccgaattt tgatgttcgg aatagccaag gccagtcaat acttgtcgac
     9241 taatgtcagt gactgcaaga gaagccaaca gttcagcatc ttttaccagt tccatggatg
     9301 tttttccaat tcgaatggtt gtcaaactca aataataagc taattcatcc attcgatttg
     9361 gtgcgaatcg aagatctgat agtgaaatcg ttttatctag caaatctcta actttggcgt
     9421 aataatcaat ggaaagggtt gcggttaaat acgtgagccc aaatcttcca gcaagtgact
     9481 gcatcaaacg agcagtgcga ttttttgctt ctcttcttaa tattgcttct gaccctagca
     9541 gcctaaaacc agttttccta gtatacgaca gctgacaatc ttccacttct aattcatttc
     9601 ttatcttttt gatatctgac aggactgtcc ctttagaaac ctgaagaaaa tcttgaaaat
     9661 gaaaaacaga aacagacgtt gcagtcgaat aaaccatcaa ataaagaaga tctcttcgct
     9721 cttgttcggt atacacagct tcaaatcgag taatatccaa aaatttttct gcccagtcga
     9781 ttgctcggaa tttctttata cggatacggg tatcatccac atattcggca aaaggagtta
     9841 attgtttgat cgcctcgctt gtatttactt cgatgatctc tgctgctttt tctagactga
     9901 tttctcttgg tgttttcagg agataactaa ttttgtagtt cacagcgctg ttcacctcct
     9961 acttatattc tagtatggta gcgcttttaa atcgaggggt gtttggtaca acttacagtt
    10021 ggattttttt tacgactgta ccgaacgatt tctatcgaat ggatacagaa aaaaagcagg
    10081 tgaccaaatg tctcctgctt ttatgaattg atttcgtccg atcaatgatg atcatgttta
    10141 ttttttgagg tcagttcttc aaatttttct ttgacatctt caaccgcttt ttcgatatct
    10201 ttttcaaagt gattgggctc attaggcttt gtttcctctt ctacagaatt tgaaccagat
    10261 aaagctgttt ctttattttc tgtttcacca gagtttgtgc cagctaatga ttcttctttg
    10321 tcttctgttt caccagaatt agttcctgct aaagccactt cttcttctgt tttaggggcc
    10381 attgaacctc ttaatcgttt gtgtttttca ccgtcataat ttgtcatgtt ggttcctcct
    10441 agtagttcta tactttttca tcgtacaata agacagttta tgcgtcaaaa aacaaacccg
    10501 attcttacaa atatgtcata tttttccttc tgattcttga atgcttttga ttccttcttc
    10561 caaaattttc atttgtaaga ttgtttcttc atctttgatg actttttctg cgccattaac
    10621 taatgtctcg taaatccctt cgtagacacg gctgtaatca ccaacctcgg aaattacttt
    10681 ttcttcatga taactccctt gttcatctat ataggtcaat acaccataat gttcgggcaa
    10741 atcgatacca aagtcttcat gatcaggcat gtaaaacatt ttcagatgtt cttcttgtcg
    10801 atcttttgtt tgtttcacga acatcccttt ttttccgtaa acgacgaaac ttggacgttc
    10861 tttgatgcgg aaataactgg attttatcga aattttcata tttccataga aaaaatccag
    10921 atcgaagtaa tcattcattc gtcctttccc taaaagttga cgtacatcat agctgatatc
    10981 atctggttcg ccgaaataag aaagaacttg atctactgta tgacaagcat gtccatataa
    11041 atagctgttc gcagtagaaa attcatgcgt attttctggt acttccggtc tgaaataatc
    11101 ataatgcatt tctacttcta aaatatcccc taattttccg ctttcgatta ctttttggac
    11161 tgtcaagaag tcagaatcga accgtctgtt ttggtaacat tggataaata gacctttttc
    11221 ttttgctaat gcaaataact cttctgcttc ctttgacgtt tctgcaaaag gtttctctac
    11281 tagtacattc ttaccatgtt cgagtgccat ttttccatat tcataatgaa attgactagg
    11341 tgtagtgatg acaactaatt ggatctcagg atcatcccaa agatcagaca gctgatcagt
    11401 atattcgatg tcttcgaatg ttttccacac tggttcagat ggggaaaaaa tttttttgat
    11461 attgattttg ttcgtaagtt ttcttgaaaa aggcaaatga tagcgatttg tactctttcc
    11521 attgccaaga taagcgattg taagcatgag tggtgcctcc ttttattgat gttttcagta
    11581 tatcctagaa aagcgcagaa agtatttata atagctattc tggtaaaaaa gttcagaaaa
    11641 acacttgtta tcattcggtt tggtgaaatt aatgttcaaa gtaaggataa aaatcaaaaa
    11701 gatagtagcg gaaccaagtg tttatcagta agatagaatt tggaggtcag atcatgctta
    11761 ttgaagaaaa actaacaaaa caagagttgt ttaccacgac agagaaacgg attgtcgatt
    11821 acatccgtag gaatatcgaa gctgcagtct acatgacgat tgaagaatta gcaaaagcta
    11881 cgtatacttc ccattcagct atcattcgtt tatgcaaaaa aatgggattc agcggattta
    11941 aagaattccg tttcgagctg gggcgggaag ttcatcaatt gattgctcag ttcaatcaaa
    12001 cgattgatcc taattttcct ttttcaagtg aagatagcac tcagcaaatt gcaaagaaaa
    12061 tggcggattt atcgatccag tcaataaaaa aagcacaatt gcagattgaa catgaagatt
    12121 tagatcaaat cggtaaagta ttgaacaagg cacagcgaat ctttcttttt gcaaaaggtg
    12181 attctcaaat cacggcaagg aaattccaga ataaaatggt caagttgaat aagtttttga
    12241 tcatggctga agaatacagt gattcttctt ggaatgcagc caatttgact agcgaggatt
    12301 gcgccatttt tattagctac agcggtcgca tccatcatta tgaacgaata atgacctatt
    12361 tgaagcatgt agaggcccct accttgctga ttaccggaaa tcagcattca gaaatggcca
    12421 aacaggctga tatgtgtctt gttatatcgc aggaggaata tgattttgct aaagtagcga
    12481 ctttctcctc tcaaattgcc ttcgattacg tattaaatac gttattctca gtgatatatt
    12541 ctcaaaattt tgaagagaat atccgcaact tgaaaaacaa acaagaattg atgcaaaaag
    12601 gccttcttaa agaatatcgg aaaacttaag aaaagatcct catttagtaa taagaaagat
    12661 agtgtttgat aggctctctt tgatatcatc ataaaaacag gctgaagttt tctttttcaa
    12721 acctcagcct gtttgtttac ggaacgattc gaatagatat attatagaaa tgaggaatag
    12781 ctaacttctg tttcttattt tgaacgattg acgaaaaaga tatataagaa ggaggaaaat
    12841 cagtatacat gcagctaaca atccatacag gaaaaatcgt ttgttctcgg atactttctg
    12901 tacttctttt tgaacttctt ttgcatgata aggaacccgg tgccctctga caagtaagcg
    12961 atgactgttg accatataag gtgtacaagt taggagtgtc acgaaatcct gccctttttc
    13021 gattctcaga tcttttgtat cagtcggttc taccgtcttg atctgatcga cttgataagc
    13081 gagtgtccgt ccattgattt cgatatagaa agaatcacct aaagtcagtt tcggtaagtc
    13141 tgtgaacaac tttgcttgag gcagtcctcg atggctggaa ataacggcat gtgtattttc
    13201 tccccccgta ggataagacg ttccttccaa taatgaggca cctttttcta gcagcacagt
    13261 attcgtttca tcaaaaattg gcaaatggac attgatctca gggatcgtca aaataccgat
    13321 cgtatgtttc tcaaaatagg ttctatcctt tttcgcaggt tcttttttct tagtaaaggg
    13381 atctgcacct ggatttccac cttcttttgc taattgctgg tttttctttg tcatgttttc
    13441 ttggatttta gccatgactt cttcattttc tttgaccgct tgttgctgat aatgagagat
    13501 gatctgctga tccagataat tattcagcgc gtcgctaaca aatggataag ctagtatccc
    13561 tattcctgag agaagcaaga aaagcatcag cagatctaga agtttccttt tctttggttt
    13621 catttgtgtt ctccttgtga tgactgtttg ataataaaag aaaaaggagc atttgcgtaa
    13681 tccgctgctc ctttttccaa ttcttgcaaa aagactaaat ctgactgtgc ttgcgtctag
    13741 caaagtacag tccagcaatc agtagaagga ctactcctgc accgatatac acatagattc
    13801 ccttaccgcc tgttgaagga agtgtacctt tgtgtttatt tggtattttt tcaggagaaa
    13861 tcaactgtcc tgctgttaca taagattgtt catcgatagt aaatgctaca cggtttgtta
    13921 atggaacata tttttctggc gctttcgttt cttccagata gtacgtgcca tattttagac
    13981 ctgtcacatc gattaaaccg ttacttgtgg ttgtaaaaac cgttgctgat tctttcgccg
    14041 atacccagct gacggctttt gtggatgggt cgatcgataa atatttcgct gtgtcactat
    14101 cttgatcacg aacgacgaat tctgctccag caagtgtttg gtctgatgta acgtcaccat
    14161 ctactttaac aaatcgtttg cccccagtaa cgacatcgac tgacggtggt gtttgatcat
    14221 ttgtatgccc gttatcgaca tttgcttggt tagaaaatcc tttggttgga tctgcttttt
    14281 cgttcaaatg catatagtaa acgaatttca atgtaccgcc aggagttaat gaaggaatat
    14341 aattcggatc aactgaaacc gtgaatccgt ctgtttgctc agtgacagaa taatttactg
    14401 ggtcgatttc tttatttcca tcatataagg cataagcata cgttcctgaa gaatcattat
    14461 caaatgttaa agcagcgtca tgagtatcga taagtttgaa tgttgtgtac ttgttttgcg
    14521 ttccttcttt atcagcgata cctaatggga tattgacaga aatctcatat tgaatttttt
    14581 caccgattgc gacatctttt ccattcaatt gaggtgttgt tttatcaact ttagacgtat
    14641 catttttgat ggtcttttct actggtgttt ggctatttgc caggatctca aaaggcgtga
    14701 ttgtttgctt ttcaatcatt tccgcattat caggagcttt tacttcttct aaattatatt
    14761 ttccaacttc taaatgatta acgatcaact ggcctttttc aatagatgcc tcggcaaagt
    14821 cattgttgcc gatgtcataa gaatgaccag taatgaaatg tttggctttg gtttgatcag
    14881 ttgtccaagt gtacaatcca tctgtgacac tttggatgta ttttttgacg cttggtgttc
    14941 cttcttcttt agaaataata aattctgctc catttagtgc ttcgttttcg gcagtaccga
    15001 tttttgtaac tttcaacgtt ccatcattac cgactgtatt tttagggtag agatggatag
    15061 tatctagttc ttctgtcccg tatttataag agccatctgc ttgtttgatc atctcatata
    15121 cagggaaagc taaaaccatg tttgcggcag ctgacactcc gtcttttggt tcttctttga
    15181 tcgtgtagac tgcatctttc ccattttgtt ttttaggtaa agataaagtg acatttccat
    15241 cagcatctgt cgttcctgaa gcaactggtg taccaggagt caaagactgg actgcttgtt
    15301 ttgctgcatc cacggacgct cctttatctc gttgcgcata aaattcttga gtgacgttat
    15361 aaactgaaaa tgaaatatcg gctaatcctt ggtattgatc gaattcgctc atttctttcc
    15421 cgctgttttg gattaaagga tcgggtaaat cagtcatttt ctttttatga aggatgactt
    15481 gagctgctgt ctcctctgca aacacttttt ttgcgccgaa gctgtttgtg agtaatggta
    15541 aaataaggaa aaggactcct aacataacgt ttattttttt atgatttttc attttttcac
    15601 tcctgttctt tttttgataa acaaccagat tgcaaagctt gcgaatacca taccaacaac
    15661 gcctaaaagt gtatttttcg tttcgtttgt ttttggaaga aatggacggc tattttcttt
    15721 tttattgact actttcaata tactgtccgc ctctttatcc tcagaaagaa aagttgaaat
    15781 ggtaaacgga atttctgttt cacttaaaat ataaccagaa ggtgcttcga tctcttttaa
    15841 tcgataactt cctgttttca acccggaaat tgaaaaagcg ccatttttat cggaaatcag
    15901 gtgaagcgca tctgattcgt tctttgtcca cttgtatccg ttggctgttt gctggaggta
    15961 atttcctgct ttgtttttaa taaggaatac tgcgccagct aatcgtttat ctggtttatt
    16021 tgtctcgatt ttgacaaatt gcttactccc tgtatataca gagactttct ttgtactgac
    16081 accaaaacca aaatccagtg ttccgttgtt gttgatttcc ttgttcgcct gtgctgtact
    16141 gcttagctgc atctgataag aaatggtcag tttttttcca gcatattttt ccaacgaagg
    16201 gatcgaaaat aaaacatcaa atccgtgagg tttcttgtgt atgacaaagc cttctgtcag
    16261 cttttctcca ttcgatgaaa tcgttaaact attaggtaaa aacgttaatg cttcatccgc
    16321 actatctgac aattcgaact tttgatagtc aaggatattt acaggaattg tcgtatgcaa
    16381 ggaataagtg attttttcac caatcgtaaa atcatttcta ggctcttcta ataccttctc
    16441 aaaaggtgga tcagggtagt cgttttcttc atttttagga tacaaatgga tagttgaaag
    16501 tttttgattg ttttgtccat gtacaggaag aacaactacc atattctctg ccttttcttt
    16561 gacgacttct ggtactttgg attcaatgaa aagatacgct ttatctcttt tttcttgatc
    16621 tttagcggcc agagaaaaag aagcgatacc atcttcgttg ttaagagttg tagttgttgc
    16681 ttctgcggta aacataccgg aagacgcacc gttttttgcg atctctgctt gtgcttcttc
    16741 taccgttttg cccttttccc gtagatggta aaaagaatct gtgacatcat aaacttggaa
    16801 tgtgacacca tttaatcctc gatacgtttg taataaagct ttttcttgtc cgtcatttgg
    16861 atgatttttc ggcagttgcc cgtttgggaa aagcaatttg tgtaaagtaa tctgaaccat
    16921 ttctgtttct gtttctgttg ctacctgagt aaaagctggt ttaaatagta acaagaagag
    16981 acacatacta agccaaccaa gttttttcat atttagctgc cccctctttc ttttttcaaa
    17041 aaaagatacc aaatagaaaa cgcacaacca atcatcccta ctagatagat tcctaaacgg
    17101 ccaattcctc ccgtttcagg taatggtact tttgcctgat tcgtgatgtc taaagaaatc
    17161 tggttgtttt tggctcctgg tgacagaaca gattcatgat cttgtccatc cacttgaatt
    17221 gacccatctt cgtgtataac tatagtaact ggctctttta gaccttggta tccttctggt
    17281 gtaaaagttt cggtcagcgt ataagttcca ggttttaaat tttcgaatag aaaggtatct
    17341 gtttcttgtc catcttttgg cgattctaag tccatttctg gtccctgcag tctgaatttt
    17401 gctcctttta atgtctgtcc gttgtcttct tttttgtgta ccgttagatc aaacgctttt
    17461 agtcgatttt gatgagtgag cgtccataca ttatttgtca tctcggtatt ttttcccgtg
    17521 taaatgattt ttccatattt atcgatttgg aattcataat ttcccggcga agtatcatat
    17581 ccggcaggac cagctgtttc aacgactcga tatttaccag gtgtgagtga atcaaaactg
    17641 gcaagacctt tttcattcgt tgtttggctg tcaattggct gataagtacc atttgcttct
    17701 tttttctgta gggaaaatgc tgctccttta agatttatct ctttgtccgt cccttgcatc
    17761 gcatacttac gtactgctac aggaacttca acaaaaggat tttcaatagt caactggatc
    17821 gtatcatcgg aagtagtgac tgtttttcca tcaatggtta ccgtaccatc agaagcgatc
    17881 ttgatttccc aagtagtctt tttgcttaat ccatgccctt ctggagcttt tgtttctgtc
    17941 agcgtatagg tcatttcttt ttgcaatttg acattttctg gaagagaata ggttccgtcg
    18001 ccatgatctg ttagtaaagt atcaatagaa tctcctgtta atttgaaaac agcgccaata
    18061 agatcctttt cacctgtaga ggaatttttc gttattttca agtttaacgg tgtgaattgt
    18121 ttagtatttt tccatgtcat tgcatctatt tgttgagaat cgtatcctgg tacaggtaat
    18181 tctttgattg tttggtaact gaatgcttga ccttgattat tgtattgagg tagtgataat
    18241 atctcttgat aactttctcc gctatttgca gataatttgt caatgtctgc acgttcccac
    18301 gtatttgttg tatctttagc tggtttaatg attcgaatat atccgttttt ccaagctgca
    18361 gcatttgtcg tatgttcccg ttgaatctca aaagtaactt gatctggacg atcagctaga
    18421 ttgttgtcaa attcttccca taactttttg atgtgaagac tgactccggg agctttagca
    18481 gatggtatac cgaattcagc taattcattg gtatcaatgt ttggctggaa agttgtcctg
    18541 ccgttcattt gataccagaa atttggatgg aagtcctcat tttctgtttg gattctcact
    18601 tggtaatgga tttggatttc ttggtctttt cctaaataga tctgattgct cttgatggta
    18661 tttccttcta tggaaataga tggagtgacc gttgtaggac ttgtccccac actcttgact
    18721 gaaagtgtac caggctgata aatgaatggt tctgcgattg gatcatttat ttgtccattg
    18781 cttacagttg ctgagatctg tacagctttt ttggctaggt attcagagat atctgttgca
    18841 tgatcagctg attcatagta aagatcgcct ttttcatctg atgaaaccat ttgtcgcata
    18901 cgagactcta cttctgcttt tgagagacca gctgccggat cgctttgaag ttggatgcca
    18961 agaccatgta tttcgattcc tcgttctttg agtgccatcg cttctccgat ggttgcgata
    19021 aacgtactgt cgattcttct ggatagattg ttttggtcag gtgcatcata gattcttgag
    19081 attagagaag tatttcccgg ccgatcttgc gtattagaaa actgagttcc gtaataattg
    19141 ctgcttgatt gtgcgtgtac ccgctgtact ttataggaaa atgttggtac accatccgtc
    19201 agcaaaacga tcactttttt atgtccattt ggaacggata gcatgcttcc tgcatctctt
    19261 agtgcttttt gtgtaaaagt accaccattt gtccgtgaag gtgtaatgga ttttacttga
    19321 tttttcactg aatcaaatga acccatctgt actgcaccgt tactgtagct atatccttcg
    19381 cttgagtagc cgacatatcc catattgatt ttgtctgtga taccgctatc tgctaaagta
    19441 tcgacaaaac gatcgacacc aatctttact tcaccgatcc gattattgtc gttcatactt
    19501 cctgaccagt ctacgaccaa tacgagatca agaggcgtga tatctttttg tacatttcca
    19561 cgagcattca aataaacatc aaacaatccg ggtgtacttg tttcctttgc atactttcgt
    19621 atcgcatagt ctgcttgatt accggttcct ccatattcga tatacgaatg ggtccgatca
    19681 ttaggatctc cattccaaga atttatacca tcccattgat ttgttccgtt tttattgcct
    19741 tgatgatcaa gaacatttgt atttcctgta ggttgccaac tggctttagg ataagtgccc
    19801 tgatcatctg ttgtatattc tgtaggaatc gtttgattag atggcacact cacagctggc
    19861 aaggataatg taggcaatgt aaatgggctg acagaatttt cgttttcttt tgatgattct
    19921 tcttctattt cgtctggctt tggtactgac acatctgtat ctttcttatt ttctgtctca
    19981 tcaataatgt catattcttt attatcgacg atcagcttgt ttgcctggct gtggtaaagt
    20041 gctggatcga ttggaaaact cagatcgata gtttgtttag tgtttgatga gatttccaac
    20101 gtaattttcc cgctatttac ctgataagca tagccatctc cttcttttct ttccacatca
    20161 cttaagtaat tttcgactag accaatttct ttttctatct tttcagcact ggtgttattc
    20221 gcatttagct tgatgttcag tgcttttttt ccagactgat ccgaaatgac ttcagctgaa
    20281 attgtttcta aaactgttgg atcatctgta gtgtctgcta ttgcagataa tggcacaaag
    20341 tttgataaac tcaatattat cagcatgaaa ataactttca gtttcttccc tgtggtcgtc
    20401 atttttcccc tcctttttct ttttttctgt tttgacgtga atagtgaaaa ttattaaaat
    20461 aaacaggcgt tacataattt tatttatttt aatatgataa ttattatacg aagggcgcta
    20521 aagggaatca caccaatttt cataccaatt ggtttcattt atgaagttga ttccttcgat
    20581 taatgagcgt ttcattgaaa gaaaagggtt gagggagaaa aacatatgat caggttgttg
    20641 gaaggaaact atagaaagat ggaaaaaatc attcgaattc tgtccatttc tgacaagtgg
    20701 atgactaaaa aagaacttgc caaacgaatt ggtagttcag aatctacatt tgtaaggtat
    20761 atcgaagaga tcaaaatccg ctggggaacg gatgtaacga ttcaatcttc acaaaaattg
    20821 gggtatcgtc ttgaacattt gaatgtacca gcttatttaa atgttctgat ggaaatgtcg
    20881 caaagttcta caaacagtcg tttattaaat gagattattg tcaatccggg taaaacgatt
    20941 gagtattatt gtgaaaaaat ttccatcagc cggtcttctt ttacacgtaa gttgaaacaa
    21001 tgcaatcaaa ttttagaaag ctatttatta aaagtgatcg ttgaccaagg ttttcagtta
    21061 actagcctta aaagtgagct ccagttgcgt atctttgtta cctttttctt tttaagttac
    21121 tacggaagac gcacgttgcc atttccaatc gaaaaaaaga agatcaaaga aattatggtc
    21181 aagaataatt gtcagatgaa tttgtttcag gaagaaagcc catatgagca atctttcttt
    21241 attatgtatt ttgttgtgca tctcatgaga gaatcccaag gaactccatt tgaatggaaa
    21301 gctggagact cttatgtaca gttgataaat atcgataaaa aagacttctt aatcttgaaa
    21361 aatctcttag gaaaattgaa gtacagcagc tatataaaag tagtgaacta ttttgtcaat
    21421 acgatttttt cagaattttc ggagaaagaa aaaaatgaaa tcagtgagca gatacgaaaa
    21481 aatatgtcct actgttcgtt ttcatccgct gattgcaaag aaaaaaacga agaatatgtt
    21541 gttcaattat taacgcaaat ttattttttc tcccgtattt tcccatacga tacgactcat
    21601 ttgacgatcc gtgcaactta ctttgcacag cagatgcaac accgccagat acgtctatat
    21661 gctggatttc gtgcagaact tcaattttat tccactctac tgaatcagaa tctgatttat
    21721 tatttatcta gctatatctt ttggatgctt atcggtaatc ctgaaattca ccattatact
    21781 tctgataaaa aaatactgat tatcagcgac ttaagtcttc gacatagtca atatattgaa
    21841 gaatatatca gtgatattct tgctgtgcat aaaattcact ctgagacaac tgctattact
    21901 gaagatcagc tgtcgaaata caatcttctc gagtatgatc tgatcgttac gaatcaaccc
    21961 attttgaatg cccatgttcc tcatatattg attgatgata gtgtgtcatt cgcaaatgaa
    22021 gaagaactca tccgcttatt cgagttatga ataatcaaaa aacagctatg tataaaccac
    22081 cctgataaaa atttcaggac aaatacacat agctgtttct ttttattgta ttccttcagc
    22141 aatccaacga gatttatagc gaaagtcttt taacaactct tcttttgtcc aattatttgc
    22201 aatgagatcc gtcaaagtgt tcattacgag ggtcatggaa aagtatagga aactaactgc
    22261 ttcttccgac gattgaatgg agaaatgatt gattcttaat aattccagcc atttttcgtt
    22321 cattggagaa tcagccgata gttctctacg tttggttgtc tgctgatcat ttcgctgggt
    22381 taacaagtat aacctttgcc aatagatact ttcgtgatct aagtcgcagc accactctaa
    22441 atatttttcg atccccaaaa agaaatgttg tttgttttcg ctaatgaatt ttaagatatc
    22501 ttgatggact atgtgagaac tgcttttgat cgtatacaaa taagcatctt ccaaatcttg
    22561 aaaatattta taaaaagcac cgcgcgacat ccccatcttg tcaacgattt ctgagacttt
    22621 gacttggctg atgtgctgtg agcaaaatat ctccagtaat aggtcatcga ttctctgttt
    22681 ctttgcgttc gataaattga aaaaagtttc ttttggcatc cacatctctc ctttatctct
    22741 gattatacgt ggatagccct tcattgtcat gaaggattca ttccttcttt ttctcttctt
    22801 ctgtcaagta cccatcttcc attcgataaa cgcgatcact ccattgaatc atacggacat
    22861 catgagtaac cataatagtc gctttttgtt tctcatgagc ttctttaact agtaatttga
    22921 cgacttcata tgcatgatct gtatccaaac tagctgttgg ttcgtctgct aagatcaagc
    22981 ttggctcatt atataaagcg cgagcaattg ccactctctg tctttctcca ccagaaaggt
    23041 ctttaggata actattttta agtgactgga tatctaaaga ttgcagcaag gaatcgattt
    23101 ttgttgaatc ttcttttttt gaagtaactt ggtcaactag ttcaaattgt tcctttaccg
    23161 ttaaaaaagg aatcaaatta gagctttgga gaataaatcc tatttcttta aagcgtaaat
    23221 ctgctcgttt tttttctgga agctcagtta gtgagtgacc gttgatcact atttctccgg
    23281 aagtcggtgt ttgcaatcct cctgaaatgg taaggaaagt acttttacct gaaccagaag
    23341 gaccaatgat gctgacgaat tctccttcct ttacataaaa atcaattgct ttcaaagctg
    23401 agacctttgt atgaccagaa ccaaatgttt tggtaacttt tttcatcgtt aatatttttt
    23461 tcatttttat taacctccta ttgctgtaat tgggtctact ttggttatcg tacgtatcga
    23521 aaacaatccg cctagcattg cgacaatgat caaaatccca ctatatagca accattgtga
    23581 ccaaaaaaca gcaaaaggca tcgcgcttgg taaaatcaga ctagtcaaat aagcaaacaa
    23641 aagagcaagc agaacgccta ccaccccaac aataaaggac tgtgctacta aagatttagc
    23701 gatgaagaaa tttttgatcc cttgtgcttt cattactcca aaaatagcag tcttctgaag
    23761 tgtcatcacg tacatgaaaa taccgaccac ggcagcaacc actaagaaca aaaagtatat
    23821 catcgcattt aaggtcatat tttgggcaga atagccagga atactttcaa tgaatgtccc
    23881 aatagttaat aactgtagac ccttcgatgc tttttctttc gaaattttag ctgaatcctt
    23941 tattacgatc ccattgatcg gctgttcttc ttcagaagca aacggctgtt gtccatactt
    24001 caaagcagtc cacgtatcta gggacgcata gatgacggga gaaactgtgt aataagtttc
    24061 aggaaagata ccgacaatcg tcaaaggatc gtcgtaactg ccaattttga tcgtatctcc
    24121 aattttatag cctgaatcag ccagattttg agagatgatg atttcattct ttgttttaaa
    24181 ttcatgccct ttcgtgagtt ttggtaataa aaatgcttct ttcgttgtgc cgaaaactgt
    24241 aacattttct ttgttttttc ctttgattgc tccgctgtac aatccgattg gtgctttctc
    24301 tcctccttca atgtatttca gatctcctct tgttaattgt gaagctgcaa atacttgatt
    24361 tgcatcctcc gacaaaacaa tctcttgtgc gtcccaatca tcgattgctt ttttaaactc
    24421 ttctgaaagt ccattcgcta gtcctgacaa catgaatacg acatacgcaa tcaagaacat
    24481 aataccgacg atcaagccat atcgtaattt agaatagcga atttctttga tagctagaaa
    24541 catttgtttt tcctccctat gatagattga tagtgacaaa atgtcacttg ttgaatagtg
    24601 taactctaaa gtgacaattt gtcacttgtg ttgttttttt cttatcaata aattgtctaa
    24661 gacatctttc gataaaacct taacatgagt cataaaaaat ttatttaagc cttatttatg
    24721 ctttccatga taaactaaag tgaccaaatc aatgaggtgg gaaaatgatt aaactattag
    24781 tagtagaaga cgataaagta ttatccaata atataaaaga aatactcgct gcaatcggcg
    24841 agataacaca agtttatgat ggtagtgaag gactgttcga gttggaaagt ggagtttatg
    24901 atcttgcaat cttggatttg atgatgccgg aaatgaatgg atatgaagtg ttagcaaaaa
    24961 tacggaaaga aaaaatacag actccagtat tgattttgac tgcaaaagat agtttggaag
    25021 ataagattga aggatttgaa cgaggtgcag atgattatct aacaaaacct ttctatcgag
    25081 aagaactgat catgcgcgtg aaagcattat tgaaacgctc gctaggtctc tttgatgaaa
    25141 atgttctgaa gtataaggat atcagttgtg atttaaaaag caatgaagtc ttgtataaag
    25201 aacaagtttt gcccatacaa ggaaaagagt ttgaattgct cgtttatttt ttacaaaata
    25261 agggaatcat tttgacaaaa gagcagatat ttgaccggat ctggggattt gattctgata
    25321 cgacgattac agttattgaa gtttatatga gcaacttacg aaaacatctg cgaagtactg
    25381 gattggataa agaaatcaaa accttacgta atgtcggtta tatactgcaa agggtgtgat
    25441 gcaatgaatg gagatattac acgaaagcaa cgtagtcgct tttttgcgac aaatgtagca
    25501 gcatttgctt ttatcttttt tcttttggga ttcattactt tacagatatt aaaccaatct
    25561 gcatacagag aaacagatct gtcattagag aaaataaatt ttgattctcg aacagtacaa
    25621 ttagagatac aaagatatca gcaaggcgat ccctttttaa acccgactca aacggaacaa
    25681 aacagaacaa acatggaaca gccattaatg gaaccatcaa atgaccgttt taacactcaa
    25741 gtgatcttgt ggtctaaaaa tggtgagata ttgaataaag cagctattgg tgggcgtttc
    25801 aaccaaatca aagaattaaa aatggatacg aaaaatctga ataccatcca aaatattgaa
    25861 ttaaatgaag acagtggctc atctgccctc tctttccgtt cgattacgaa aaaagcggag
    25921 acgaatcaag atggcgttgc ctacatccaa attttagaaa atacaaatca aatcaagaat
    25981 tcattagcga cttttcgcac gattatgatt ttgtgtatga ttgttttttg gattctctct
    26041 attggtatca gttattactt gtctagtcta agcatgcgcc ctattattct ttcttggaga
    26101 agacaaaaag aatttgttga gaatgcttct catgaattgc gcacaccgtt aacgattatt
    26161 caaaatagtt tggaacatct ttttaccaag cctaatcata ctattattga agaatccgaa
    26221 agcatcgctc aagcattgtc agagacgagg cgcctcactg gattgacttc ggatttgctg
    26281 acaatcgctc ggaatgattc tgatcagcag ctgctttcaa agcaaatgat caatacacaa
    26341 gaatatatcg aacaacttgt taaaccattt caagagatgg ccatcataga tggaaaagag
    26401 tttattctcc ataacgatgc aacattagaa gtaccaattg atgaaaaaaa aatccatcaa
    26461 gttttggtta ttttgctgga taatgcatta aaatatacga atccagaaga taagatcatt
    26521 ttggattcat ctgttaacca taaagaatgg ctgattcacg taaagaatac tggcccttct
    26581 atcagtgaag aagatcaagc aaggattttt gatcgatttt atcgggaaga tcaatctcgg
    26641 gcgaaagaga ctggcggata cgggcttggc ttggcaattg caaaacaggt aatcgaacag
    26701 catcatggac agatttctgt atccaattat cttccacaag gagtcgattt taaagtccaa
    26761 ttgccgttga agcctaaaaa ataatgctta tccctaaaaa agggctgtga caaaaatctt
    26821 gtcacagccc ttttttgttg cgaataaacg gtgttccaga agtagcttct tcggaaataa
    26881 gccgaaattt ctttaagctg accacttctg tcacaacctc tttagcttac gcttacgatt
    26941 ttttattgcg gtcttcaatg tgtacatcac cgcttgtgtc aatatccaat tcttctcggc
    27001 taacatcttc tgtcactttt tcaacatctt catgttcttc tttatgaatc ccaacttctt
    27061 cacgaataac tgtatgtttg cttacatcta cttgctcttc tttgattggg atagtaatcg
    27121 tgtcatcttc caatttttcg tctgttccct gtgaagattg atctgttact ggtttgcgtt
    27181 cgatgacgat ttcttcacgt ttgaccggta cttctaccgt ttcagtatcg ttgaccacat
    27241 gtttgtggat gtcgacttct ccagttgtca catcatgtgt atttacatct aatttttctt
    27301 cttttagttg aattgtatca tcatctagat ttggtttgcg atcgatgtct tcagtcttgc
    27361 ttgtacccat tgtatcagtc tcgccaggta cgtccatcgg atcagttcct ggtgtcatat
    27421 tcaatggatc cgttgtattt tgaaagcctg tcgtgccacc tacgtccatt ggatctgttc
    27481 cagtgccagt ttctaatcca gtccccattg tgcttgctgg gctaacagta tcttgtgtat
    27541 ccattgtatc ttctgttgtt tctggctgac gatagccttt tactgtgatg acaaattttc
    27601 catcagaaat atctcctcta tattgatata aaggatcatt ttctggtgta gccgtctcag
    27661 aatcataggt atcaaaagaa aaagtatctt ttattttttc ccataaagaa cggtcttctt
    27721 caccatccat ccgattttca tctgtttcaa catctatacc agacaatccg tcaaatccta
    27781 gtctagatgc tgcttcatca gtagtataaa taacgatatc atctcgttca tacccttcgt
    27841 tacgcaaccg ttcaattact gataatgctt cttccctcgt actatagctt ccgatgaccg
    27901 ttaaattttt ctcttccatg taaattcctc ctagcaaata gatttatgat tggtatattg
    27961 ttacttataa atttattcta taggagacga ttgttttcta caaatattta gattggaatt
    28021 tgattaaaac aatttaattg aaatttgaaa acatttatgt taatgaaagt catattctaa
    28081 taaaatgtca tttgtttcta tatgaatttt tatgaaaaat ttcatataat ttccactcgt
    28141 tgtgatggtt taatttatcc aatccatcaa taagcaaaca acttatataa aaaaggatta
    28201 attctcaata taattgctcc taccatagaa taaatcttca tgtaaagtaa tgaagtacca
    28261 actatttcta atacttcttt aagaattatc ccaacaatat cgttagacaa accattattg
    28321 aataaatgga gagccaatcg attctgaaaa aatgttttct aatttttcgg aaataaaaga
    28381 aagagcctcc tgccattaga acgaaccaaa tccatttcat tgctcatgaa gtaaccacat
    28441 agctaaaaag aggataaatc aattaaaaga tcaaaagaat gactgcacta ataaaataac
    28501 taaaaatgta tcttcttact tcatatcttt tagctagtac ttagttaaaa atttgcttcc
    28561 taagatcaag acaaactgtc tatcttcatt tgatgaaatg cggtaaaata tagaaaatga
    28621 acactattgg agggaatttg taatggacag caaaactaga aaacaactgc aagaaaagtt
    28681 aaagcaacgt acacacttgc tcccttccgt ctttcaacag tttttagaag aaaatcgttc
    28741 ttccctttca ttaggcagtc gctatgaata cgcaaaagat tttcatctgt ttactgatta
    28801 tttgagaaaa gtattttctg aagaagtgga tgatcagctg attatccatt tagaaaaaca
    28861 agattatcag aatttcttag ctaaaatcta tcgacatact cgttcgttcc aaactacagc
    28921 gaaccaagaa acggagcaat tgtttgaaaa tagctattac gggatttcaa ggaagcaata
    28981 ttcattaaat cattttttac gatttttgta tcaaaaagga ctgattgaag aagagatttc
    29041 tcttttgggt acaagtttac cggaaacaac caaagcagct ccgcgatata tcgatgcaga
    29101 acttttcaaa gattttgagc atttgtttat cccgattgaa ggctttcaaa caagcagaga
    29161 agctagcttt gaagaaaaga atagaccgag gaaccttgca attactgcca ttcttctgta
    29221 ttgcggatta aagatccatg aagtagtaga acttaaacgt aaagatgtat ctttgaccaa
    29281 gcagattctt aatctgaata gaaaagaaaa cagactgaat aaagtaccaa taccaaaaga
    29341 agcgatgcca tttctggaga actattttaa gttaacagca caaaatacgg acgaaaattc
    29401 ttttgttttt cgttcctcac atgatcagca aatttctcct cggactattc gccaaatttt
    29461 gagcaaaatt acagtatata aaaacgtatc gccagaacta tgcagaaaaa catatcgtta
    29521 ttatgcagaa ctatatttag atgatgctga cgcggtaaat tatttatgtg gtaatcgata
    29581 cctctcatcc cattcttttc aagatatctg ggaaaagatg gttgattttt cttatcacga
    29641 attagcggca ttcgttcggg tttcttccat ataaaacaaa tgtatgatct ttttttcaca
    29701 ataaactatg ttattatata aaggattatg ttacgattat cttgaatttg tatattactt
    29761 aacaaaagat aatggagtgt gaatgatgga gaccataacg tattcccctt tgaacttatt
    29821 tacgaatttt caagaagctg cagctaactt ttccgagacc ccgattatct ttgatgagcc
    29881 actttcagct ttcccagaat taggacttga aacaacttac aaaaaatgct gcgaaagtgt
    29941 gttgaaacgg gcttatcaat tggctcatct tggagtgaaa tccggagaca aagttattat
    30001 ttataagagt tccgcttttg acacatacct tctagctgtt tctgtgtctt accttggtgc
    30061 agtgcctgtc atgacttctt atcatctacc aacaacaaca atggaagtat ttatcgatcg
    30121 tttagaagac cctttcattt tatttgatga tgagacaaaa aagcgagtga gagaaatttc
    30181 aaatggaaca aaatcaaaac agattcctat tctttattta atggaacaac ctgctttgtc
    30241 tgtttctcaa tcatttttag acaaaaatga aatttcttat atgacccata cttctggtac
    30301 aactggtatc cctaaactta tttgtcattc tgctcattct atgggatggc ggacgaaatg
    30361 gcaaaaaaca gttttcacaa aaatttcgga aaagaaatta atcgcttttc acatctctcc
    30421 agtgcattcc agattcaata ttggtatctc ttctttgatg tccatgggat ttcctatgat
    30481 gcctttagca aacgcacaaa gttcaaaagt tgttcatatg ttggaagctc accggccaat
    30541 tgcactagag acccatccga ataactttgt tcagtggcgt tttactgcta aggagcatcc
    30601 agaagctttt tcgggtatcc gatactatca ctccactttt gatgctataa acaaccagac
    30661 aatgaagaca tttttgcgaa cgtcgatggc acaggatgcg gtatttcttc aagtatatgg
    30721 ccaaagtgaa tgcggaccaa tgattcttaa ggctcataca ctagagagtt tgaaaacaag
    30781 tgatgcaaga gatatgggcg ttggactgga agacatgaca agcgctcgga ttacagattc
    30841 agttgggaat gtcctacctg aaaacacaga tggtcatata caattactgt ctaaaggacg
    30901 ggctttgact tattacaagg aagatgcacg gttccaggaa aatgtttatg gcgattggtg
    30961 ggatagcgga gattacggtt ttatggataa gcatggccac ctatttttga aagatcggca
    31021 agtggatttg atcgaaacga tcaatagtac attagctatt gaagactttt tattagattc
    31081 gttagatttt ttggcagaag tagtgattgt aagggataaa aatcattctc cacaaccgat
    31141 tattgcttta gctcctgata aagaaatgga ttggaatcga tggtgggagc aagtccatga
    31201 acttcctcgc ttgaacgtcc ctatcatccg tgattttgat gcaatcccgc gaacagctac
    31261 aatgaaagtg caacggcttc aaatcgagaa agaattaaaa tcacaaaagt cctaactatt
    31321 taaggaaaga aagatccatt ttggtctttc ttttcttttt gtttttttaa attggatgag
    31381 ataaaaatat gccaattggc tgtttttctt ttttgaaaat ttcgttatac tcttattcat
    31441 ggaggaaaat gttatgaaaa gaatcttgac aattggcggt tctgatccat ttgctggtgg
    31501 aggaatccaa acagatttaa aaacttttga gaattttggt ctattcggac taagcgcctt
    31561 gacttctata ggggcactag atattaatga gcgatttatg ctggaatcta tatctttgga
    31621 attgcttaaa cgacagttag tttcgattga taaaatgact tcattagatg gtatcaaaat
    31681 tgggttattg aattcaagtg aagcaatcat ggttgttcga gacttcataa aaacaaaaca
    31741 aaatatccca atcattttag accctgtttt ggcattcaaa gaaactgaag ctgtgatcaa
    31801 tcaattgtat atttcgcagc tcatcgaaga actttttcca cttgttacga tgattacgcc
    31861 taatttggca gaagctgctt tactttctgg tcaagctctt ccctcttcgt taacagaaat
    31921 gatccatctc gctaaagaac tcactgcttc aggaacaaaa aacattgtga tcaaaggcgg
    31981 cacaaggatg gctggagatg aagctgtgga tcttctctat tcaattgatc attatgaggt
    32041 attttctcat aaaaaaatcg acactacaac agtaaacggg gccggttgtg ccttttcttc
    32101 tgcaataaca gcgaatcttg tgttaggggc agattttacc aaagcagtag cagaaagtaa
    32161 aaaatttgtt gcccattgta tttctaatgg cgtgctgatg aacgatcata ccggaagtgt
    32221 ctggtctggc ggtattcaaa aaggaggaat gagaaatgaa aactaagcaa ttaacgatct
    32281 atgctatgct gattgcgttg acagttgccc tttcattaac aattttgatc cctgtaccgg
    32341 ctacgaatgg atttgtgaca ttatgcgagg ccgggatcta taccgctgct tttctttatg
    32401 gtccaacggg tggattagtt gttggtgctt ccagtggtct attgatcgat ttgatttcag
    32461 gatatccgca gtgggcagtt ttttcattag tgatccacgg gttacaagga tatgttgctg
    32521 gttctttcag caaatcttct actcgtatgt ggggattagg gcttttatta ggaagcttag
    32581 ttatgatcat cggctatggt atcgccggtt ggattttata tgattggccg gctggagtgg
    32641 cttctatacc aggaaacatc ttccaaaatc tgattggaat gggacttgcc tttcctataa
    32701 ctgcaagtct tcaacgtatt caagcacgga agcaatatta attgaaaaag aaggttttag
    32761 aaaatgaagt tgtcagaaaa agaattaaaa gaccaattga ctgaaatagt taatgacata
    32821 ttggctgagg cacacttgaa aaaaggtgat ctttttgttt taggatgcag cacaagcgaa
    32881 gtcgttggag gacatattgg aaaaaattcc agtgcggaag ttggtcaatg gatcatccgt
    32941 acgttaaaag agttgttgga tcctaaagaa attgcgctag ctgtccaagg ttgtgaacat
    33001 ttgaaccgag cattagtagt agaacgaaca gtggcagaag caaaaaattt cgaaattgtc
    33061 tctgttgttc ctgcactaca tgctggaggt gcttgttctg ttgctgcttt tgaccagttc
    33121 aatgatcctg tcgaagtgga acacgtagtt ggacaagccg gtatcgatat cggtgacaca
    33181 tcaatcggta tgcatatcaa gcatgtccaa gtacctgtca gacctcgttt gaaaaccctt
    33241 ggacaagctc acgtgacagc ccttcgctct cgtcctaaat atatcggtgg tcctagagcg
    33301 aattattgaa atgagtaatt gacagagcaa tctgaaaaac ttcctcgcaa agtccaacta
    33361 atgaacttta acgaggaagt tttacttgtt tgttttatat aaccgagctt cataaggtgc
    33421 tagtatgcct ttagcttcaa attctccgct agaggaattt gacaagagta atgtccagga
    33481 ctgatctgct tcttttggaa gttcatagtc agcaagtgat tctgttaagt ttaccataat
    33541 aagatattgt tcatcttcta aatatctttc atagacataa agttgtggat gttcatgaag
    33601 atattcttta taagaaccgt atatcagacc tttattggtt tgtctaatgc ggatcatgtt
    33661 cttatagaat tgcaggaccg actctggttc atattcttgt tgttctacat tgatcgtttt
    33721 ggtatttgga ttagtgatca gccaaggttc atgtgtagaa aaaccagcat attgttccga
    33781 agtccattgc atgggtgtcc tgctgttgtc tctcgttgtt tcacgaatga tatccaatgc
    33841 ttcttctctt gtcttacctt ccgcaagcag ccttttataa agtcttttcg aatctactgc
    33901 atcaacttga tcgatcgatt caaagggcat attcgtcatt ccgatttcct gtccttgata
    33961 aataaatggc gtaccttgca aaaagaaata catcatagcg attgcttttg cagacatagc
    34021 ccaaaaatcg ggacgagtat ctccaaacac tgaaacggca cgaggaacat catggttttc
    34081 catatataaa gcgttccatc cgataccatc caatgaagtt tgccaagcgc ttaatgcatg
    34141 tttcaattta aggacatcta attgcccttc tttttcttgc tgccagatat ggatatgatc
    34201 aaattcgaat atcatatcga aataaccgtc tttccctacc cattgtggtc cttcttctgc
    34261 agtgacacca cttgcttctc cgacagtcat gatatcgtac tcttcaaaaa catgtttcaa
    34321 atcggtcaaa tgttcttcga ttcctgagac attttgaaag ggtgagaaag ggttctctgt
    34381 ggcttttact gaatattcat ctttttttac atgagaaatc gcatctaaac gaaatccatc
    34441 tatcccttta tccaaccacc atcggatcat attgaatagt tcttctttca atttctcgct
    34501 ttcccaattt aagtctggtt gttcttttgc aaaaacatgc agataatact gtccagtttc
    34561 atgagaatac tcccaagcag aaccgccgaa aatcgatgtc caattattcg gttctttgcc
    34621 atctaccccg tcgacccaaa tgtaataatc gcgatacggg ctttctttag attttttcga
    34681 ttcttcaaac cattgatgct gatccgaact atggttgatg acaagatcca taatcacttt
    34741 gatccctaat tcatgtgctt cttttagcaa taaatcaaaa tcttccatcg ttccgaatct
    34801 ctcttctata tcttgatagt cacttacgtc ataaccatta tcgacaaatg gagattgata
    34861 gatcggattg atccagataa agccgatacc aagatctttc aaataattaa gtttttcacg
    34921 gatcccattg atgtctcctt gaccatcgcc attgctatct ttgaaactag caggatagat
    34981 ttgatagcct actgcttctt tccaccattt ttgttcttgt atcatatcgt ttcactcctt
    35041 taacggtccc tcttgattat aaccgattct attttagaag tatccttgtt accggtaact
    35101 agaaacgaaa agctcaacga atctgctaag tgcgttcgct gagctttatt ttatcataga
    35161 tagcgggcaa tcagttgaat catttgcttg ggaaattcat tcaagtcagt aatatctaca
    35221 aaacgacttt caccgtagat cgacttgatt tcttctttgt cttggccaat agctgcggag
    35281 acaaatagga ttccttgacg ttcatattct ttcaaaacag actggatatc ttcttttgct
    35341 tttttgcctg tataatccgg caacgctttt ggctgaccgt cactaatatt aagcaacagt
    35401 ttggttgttg cggattgtct gtttaatttt tctgctgcta aacgaagtgc agcaccatca
    35461 cgattgtttt gccttggttt catagtgact aatttttcat ttaaccattg aaatccgtct
    35521 tcaaattctt tataagaaaa caaagaagtc ttttctcttg acgaacggtc tgctgtatct
    35581 ccgtaaatca acaatgggat atcgactttc ttagaaaatt cggcaatggc aatcgctgct
    35641 ttttttgccg cttcgatccg atcatcccgt atcattgacc cagattcatc gatccgtaca
    35701 gctaccgcta aagaaggttg ctcatgtggc gggttctttt tgtcaaaatt tctcaaatca
    35761 ccatatgcta ctttgcttgc attgaaccgc tgaccttcgt attttccttt ttggtacgtc
    35821 tctgtttgtt cattatccag taactctaaa atttgccggc taagtgattc tactattggc
    35881 attacttgca tgtgtagctc acgtgcttcc ggcgtttttc cttggaaagc tggtcggtga
    35941 acgatgagac cttctttttc atgaatcgtt tccttcatga tttgtcgcgc ttctttattc
    36001 agttgcttct tgatttcctt atcatgtgct tccatgtcag aagcagacat ttcttgagaa
    36061 tcatctcctg tttgttgttc tttttgattt tttccttcga tatcgctact gcgttctttt
    36121 tgcatttgag gaggaatctc gtttttttct attgtttctt cttgtttctc ttctccctca
    36181 tttggttctt cagcagacaa cgatttcttt tcatctaagc gcagaatttc ttcagcattt
    36241 tcagtgatgt ttcgttcatt tgcttcttcg gcgctaatgg ttctttcttt tgtatgctca
    36301 gattgaggat gaccccattt ttcttttctc aatatgtcct gaagttccag tagcaatcca
    36361 ctgtcagaca aagcttgttc aagaattgcc aattcatctg aatcagtgga taatttatac
    36421 aaaacttttg gataaatcga ctggagtaaa tcttgtcctt gcttataggt atttgcccaa
    36481 taaaaataag aacgcattcc tgctacacct ttgatggcgt tagcttttgc agtttcatcc
    36541 aacaaacgga taatctctgc catctttaag agaagttctt gtttttggat catagttttc
    36601 gccatcgcac gttctgccat gacttctaag gcaggaagat ccattttttc cgcatgttgc
    36661 atccgatctc ttaaagattc atttaatggg cgatttcctt ggtaatttcg atttgtagtg
    36721 atcacaatga cgcaatctgg atggcgacga atgattcctg tcggtaaatt caacatccca
    36781 tttggttcga gcgctgagtt aagtgccaca agaacagagg catctcggat gacagtcggc
    36841 tcttgaatct ctaataaata gcctttttca atcgctcgaa cgatttcaga agggtaatag
    36901 ctgtaatgga ttgcatctgg ataatcagct tcgatccttt gaactagctc tgctaatttt
    36961 tctttagcag aaaaaggatc aatgccgtaa tggttctgga tcagtgtcag tacagcttca
    37021 agtgtatctg tttggtaaat agcttctagt aattcttggt cttgttcctt attagaatct
    37081 actacaggta ataaggctcc gaaaacatct gatttgtcca tatctgcaaa acaggtcact
    37141 tttgtatagg gaagttgcag atcagcagat agtgctttgg ctaattgtgt cttccccgac
    37201 ccggcatctc cttccaaaag tacgttactg attttcattt caggatcttg ccaattccct
    37261 ttgatttcgg aggcgatccg ccattcttct gcacttgtcc gatggctttt cggtttcttc
    37321 cagatcattt gcttttctag ccgtgaaaag gttcggtttt ctgttagtga gaaattctcc
    37381 atgcttctct tccccttttg atcatctctg tctattaata tcctagctca tctaaaagac
    37441 gttttaaaat acggaatcgt tccccaatca gttctccgtc tttttgtagt gtatcgtaat
    37501 acaaaatgat gtcttcatcg atctttggat ttttggtatc tacttctacc gtcattcggt
    37561 tcccttgtaa tccaatgatg tctacgactg gattttctgg atcataaaat ttttcataac
    37621 gataagcatc ttggaagtat aaacttcctt gcgcagctaa gatcgctaaa tctaaaatac
    37681 gtgtgattgg taattcttcc gactgtcgtg accatttttc tccagtatgt cgccatacct
    37741 tgcctgagat atcgactttt ccacgatcat tccactgagc aaggcccagt gaaagccctt
    37801 ttgcatccat atttaatgca tttcttccat caactttatc atagtcttct acaacgatta
    37861 ctggtttatg ttttaaattt gttggtattt ccattatttt aactcctcat ccttttattt
    37921 ttagtaaata agtaaattag tagtttactc atttactaaa ctatatgcga cattatagag
    37981 gattgaagtg aggattgcaa agaaaaagag cttcattgat agaaataaaa acaccatctc
    38041 gttcttttac acgctcatta gtaacatgta gaaagtccaa gatgatgttg gaat
//
LOCUS       DAJSBN010000026        37551 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJSBN010000026 DAJSBN010000000
VERSION     DAJSBN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.026"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            75..470
                     /locus_tag="ONT86_001441"
                     /pseudo
     CDS             75..470
                     /locus_tag="ONT86_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(465..683)
                     /locus_tag="ONT86_001442"
     CDS             complement(465..683)
                     /locus_tag="ONT86_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352245.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="ONT86_001443"
     CDS             790..1104
                     /locus_tag="ONT86_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2352246.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="ONT86_001444"
     CDS             1208..2386
                     /locus_tag="ONT86_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2352247.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="ONT86_001445"
     CDS             2789..3082
                     /locus_tag="ONT86_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCR2352248.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            3925..6252
                     /locus_tag="ONT86_001446"
     CDS             3925..6252
                     /locus_tag="ONT86_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2352249.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            6378..6707
                     /locus_tag="ONT86_001447"
     CDS             6378..6707
                     /locus_tag="ONT86_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR2352250.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            6747..7751
                     /locus_tag="ONT86_001448"
     CDS             6747..7751
                     /locus_tag="ONT86_001448"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR2352251.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            7748..8194
                     /locus_tag="ONT86_001449"
     CDS             7748..8194
                     /locus_tag="ONT86_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR2352252.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            8344..9159
                     /locus_tag="ONT86_001450"
     CDS             8344..9159
                     /locus_tag="ONT86_001450"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2352253.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            9365..10537
                     /locus_tag="ONT86_001451"
     CDS             9365..10537
                     /locus_tag="ONT86_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2352254.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10699..11193)
                     /locus_tag="ONT86_001452"
     CDS             complement(10699..11193)
                     /locus_tag="ONT86_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352255.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            11776..12198
                     /locus_tag="ONT86_001453"
     CDS             11776..12198
                     /locus_tag="ONT86_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2352256.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(12364..13557)
                     /locus_tag="ONT86_001454"
     CDS             complement(12364..13557)
                     /locus_tag="ONT86_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2352257.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            13781..14158
                     /locus_tag="ONT86_001455"
     CDS             13781..14158
                     /locus_tag="ONT86_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2352258.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            14146..14484
                     /locus_tag="ONT86_001456"
                     /pseudo
     CDS             14146..14484
                     /locus_tag="ONT86_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14618..15061
                     /locus_tag="ONT86_001457"
     CDS             14618..15061
                     /locus_tag="ONT86_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCR2352259.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            15322..16137
                     /locus_tag="ONT86_001458"
     CDS             15322..16137
                     /locus_tag="ONT86_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2352260.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            16147..16809
                     /locus_tag="ONT86_001459"
     CDS             16147..16809
                     /locus_tag="ONT86_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2352261.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            17017..18681
                     /gene="alsS"
                     /locus_tag="ONT86_001460"
     CDS             17017..18681
                     /gene="alsS"
                     /locus_tag="ONT86_001460"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCR2352262.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            18694..19404
                     /gene="budA"
                     /locus_tag="ONT86_001461"
     CDS             18694..19404
                     /gene="budA"
                     /locus_tag="ONT86_001461"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCR2352263.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            19534..20031
                     /locus_tag="ONT86_001462"
     CDS             19534..20031
                     /locus_tag="ONT86_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCR2352264.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            20216..20476
                     /locus_tag="ONT86_001463"
     CDS             20216..20476
                     /locus_tag="ONT86_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352265.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(20838..21077)
                     /locus_tag="ONT86_001464"
     CDS             complement(20838..21077)
                     /locus_tag="ONT86_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352266.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            21363..21677
                     /locus_tag="ONT86_001465"
     CDS             21363..21677
                     /locus_tag="ONT86_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352267.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(22111..22477)
                     /gene="ssrA"
                     /locus_tag="ONT86_001466"
     tmRNA           complement(22111..22477)
                     /gene="ssrA"
                     /locus_tag="ONT86_001466"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(22648..23421)
                     /locus_tag="ONT86_001467"
     CDS             complement(22648..23421)
                     /locus_tag="ONT86_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2352268.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            23565..23915
                     /locus_tag="ONT86_001468"
     CDS             23565..23915
                     /locus_tag="ONT86_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCR2352269.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            23896..24612
                     /locus_tag="ONT86_001469"
     CDS             23896..24612
                     /locus_tag="ONT86_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2352270.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            24612..26060
                     /locus_tag="ONT86_001470"
     CDS             24612..26060
                     /locus_tag="ONT86_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2352271.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(26130..26639)
                     /locus_tag="ONT86_001471"
     CDS             complement(26130..26639)
                     /locus_tag="ONT86_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352272.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(26629..27354)
                     /locus_tag="ONT86_001472"
     CDS             complement(26629..27354)
                     /locus_tag="ONT86_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352273.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(27577..29697)
                     /locus_tag="ONT86_001473"
     CDS             complement(27577..29697)
                     /locus_tag="ONT86_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR2352274.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            29927..31105
                     /locus_tag="ONT86_001474"
     CDS             29927..31105
                     /locus_tag="ONT86_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCR2352275.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            31121..31879
                     /gene="aroD"
                     /locus_tag="ONT86_001475"
     CDS             31121..31879
                     /gene="aroD"
                     /locus_tag="ONT86_001475"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR2352276.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            31902..32387
                     /gene="ybaK"
                     /locus_tag="ONT86_001476"
     CDS             31902..32387
                     /gene="ybaK"
                     /locus_tag="ONT86_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCR2352277.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(32458..33711)
                     /locus_tag="ONT86_001477"
     CDS             complement(32458..33711)
                     /locus_tag="ONT86_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2352278.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            33829..35178
                     /gene="gdhA"
                     /locus_tag="ONT86_001478"
     CDS             33829..35178
                     /gene="gdhA"
                     /locus_tag="ONT86_001478"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR2352279.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            35290..36636
                     /locus_tag="ONT86_001479"
     CDS             35290..36636
                     /locus_tag="ONT86_001479"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR2352280.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(36944..37330)
                     /locus_tag="ONT86_001480"
     CDS             complement(36944..37330)
                     /locus_tag="ONT86_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2352281.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(37379..>37551)
                     /locus_tag="ONT86_001481"
     CDS             complement(37379..>37551)
                     /locus_tag="ONT86_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR2352282.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT        12546 a         6489 c         7433 g        11083 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt attcttccct catagcttcc aaatttaagc aatacttcaa ttaatcaagt
     3721 tttatctttt tttttcaata atcgtaaaaa aagagtattt tttatttaaa aataaaatct
     3781 ttgtattaaa ggacaagact aaaataacct tagaataaaa agagaaaaaa agccccaaac
     3841 tttctataca tcaataaaat aataaaaatt aaatttagaa taacgtgtaa aggattattt
     3901 gatgattttt tattgagagg agggatgaaa atgattaaga aaactcaaaa taaaatccaa
     3961 cgtatatgtc aaaaacattt tgtttctaga ctcaaagctg tatctaaaag aaaatactta
     4021 catcccatta tgatttcgat catcttgatt tttactttct tagggagctt atcaagtatt
     4081 tatgtaaacg tttatacaga aacgtcaaat tttgctaacc aaacgacaga ggagacaaat
     4141 agtccaccct attctgaaat acagtccata gaaagcacca atcagtctag caacaacgag
     4201 acaacctcaa ccacatcctc gactcaactg attgatccga cagaaaaaga aatcacagca
     4261 gcaaaagcag caggggagaa agcttattca gaaacaggta gaagccagac atttagtact
     4321 gttgcggcca ctgcgaattt caacggacat accataccag atggatatac agtgatatat
     4381 gccaaagatg gagtcattgt tgcggttaac ggatcgatga gtgcggataa aactgcgtta
     4441 caaggcgtaa ccaaagcaat cgcttttatt gataaccgtc cagatatcca taatctgttt
     4501 attggctcca cagtaaatgg tactactatc acattcccaa aaagtatcta tatgggggtg
     4561 gctaccgctg gaaatacgat cgattcagtt acacccaatg cggataaaag ttattctttt
     4621 acaccctcaa catggggagg gcttcctgcc ggaggaggta tgagtacttt ttcaatcggt
     4681 atacagttag caaatgatat tccattcacg gggtactcga tcaaaaatgg gcagatcgtt
     4741 gatggtaagg gaaatgtttc aaattttcca acctatcaag cagcaacggt tcaggtcaat
     4801 gcgaatcgga caggaacttt ttcttataca gtcaatggtg gagcagcaaa aatcggaacg
     4861 tacgggacta cattatctgg aatccatgtg ggtgacaaag tggttatcac tccaacagca
     4921 gtaacgggct attcttttgc acaaacaaac ggttctttca cagccctaga aagtaacgtc
     4981 gatacggtga cctatacagg caaacaaaca ccaatcacta ttaagtatca agactatttt
     5041 ggacagatga ttgctccttc taaaataatg aatcttactt acggaagtgc tgcacaagac
     5101 ctgacgacta atacgcctac tatcaacgga tataccttta cagctgtttc agcgatagaa
     5161 acaaagaacc aatcagcaat gagtgtttta gctagtttag atacaaatgg aaatgtaatc
     5221 gtaaaggacg caaatggaaa acagatttct gaagtcatcc tatattataa gacaaatgca
     5281 acagtatcaa tcaatgcaaa tggcagtaag tactatgatg gtctttctgt tgtaccgatc
     5341 gttaaatatg cctttaatga taaaacagaa tcaacatccc ttttaaacaa tgatctcagt
     5401 attgatcaaa tcacatggaa tccaactgat ttcaaagcga tgaatgaaaa gggagttgaa
     5461 gtaactgcgc ctactgaaat tggaacctat acgtcttggc aattgacaaa aagcggacta
     5521 gctaaactag ctgccagaac aaactattta tttactatcg tgcagacatc tgatgtctat
     5581 acgattaagc aaatcagcgg tgcggtagag ctcagtggca gtaagacata tgatggaaaa
     5641 gcaggtgtcc cgtcaatcca tgtcaagcta gcagagggtg tcactagttc gcttactcca
     5701 gtacaagtgg cacttagcag tatggattac acagcagatg cacaatcagc aaaaaatatg
     5761 gttaatgctg gcagctatac aattaaacta acgaatagcg gaatagacaa agtcaaagca
     5821 gcaaatccca agttgtcatt tactgatata gccaacacat caggaactta taccatctat
     5881 aaagctaacg cagtgattac tgtcgatgac gctgccttta attatgatgc ccaaagtcac
     5941 tcgatttcgg ttggaaatct ccacgttacc ggtgttgtct taggcgaatc attggattat
     6001 accctaacag ttaacagccg gacagatatt ggatcacaaa tagtaacgat tagtttaggc
     6061 gaaggaagtg taaacaataa ttatacgatt atcgcaacag ataccgctaa gttgacgatc
     6121 aatccgactc tcttgcttcc ttcgacaggt gggataggtc tactgccctt tgtcttttta
     6181 ggactgatct ttattttttc tggcttcttt tattttatcc atcgtaagaa aaaagcaggt
     6241 gaacagcgat gaaaaaaaca aaaaatgtac gtgtggatat atttgctgtt ctctttttta
     6301 tcctactggt cttttttata ggttctccga ctgtgtcagc tagagaatca cctattacat
     6361 ttactttgca taatcgaatg ttcgaaagcg ataagatgcc aagtatcagt caaaacgacg
     6421 gaataaatat cccggataac tttcaaggag taaaagggtt gaacggcgtt actttcgcta
     6481 tatatgatgt ctcggatgaa ttttacaagc tgcggtcaga aggaagtagt gtggaagacg
     6541 cacaaagaaa actagcacaa aaaagtgata gtgaaaaaat actggcagaa aatgtgacaa
     6601 aaacagtaga cgaggaagag ggcattgctt cttttagtgc atcggacaaa gatgagcaag
     6661 gtcgtgacgc tgtttaccga tttacggaaa taaagacttc agattaagta aaagaaaaat
     6721 cagcaccatt tgtcgttgtg cttcctgtga cagattcatc taatcataaa ttgacgacca
     6781 tccacttgta tccaaagagc gaacaaaaaa tacctgctgc actcacatta acaaaaacag
     6841 tagagaataa gcagacagat tttgcagatg gggataaagt cccttatctc atcaccgtaa
     6901 caattcctga gaacatcaac gagataggaa cctatacgat caaagataca gctgatcctc
     6961 agttatggct agaaccagaa acgatcgatt ttttgattgg tggagacaag attcatacat
     7021 ttcacacaca aaaaataaaa catggttttg tcttatcaga ttctggaaaa gatcttagtt
     7081 cgtttgctgg aaaaaaatta acgatccgtt accaaatgac attgaaagaa aacagtgaaa
     7141 aaacgacatt tgataacgat gtacgtttga cgactgacaa tcaaacagta gaaacacatc
     7201 ttgccatcca aacaggaggc aaacaatttg tcaaagtgga tcggcaaacg aaacaaaaac
     7261 tagcagatgc tcaatttcta gtcaaaaaaa atgaccaata cttagcgcaa gaaaacggga
     7321 taaatcattg ggtagccaaa gatgattcaa gagcaacgat ttttacttcc agtaaagatg
     7381 gaacattttc tgtccatgga ttgtcttttg gaagttacga actagtggag attaaaccgc
     7441 ctaaaggata tatattgagt caatcaacca ttctctttga agttgaaaaa ggcagttatg
     7501 atccgcttca tcctatacca ttggaaatca tcaacgaacc aaaagcaaat ccgacgaata
     7561 atggaaaaac gccagttcag atgcagacaa atggtccatc aaatgaaaaa aatggaggaa
     7621 gctttccaaa aacaagcgaa gagatgcttg gaggtttctc aattttaggg ttattgctag
     7681 tcctttcaac agggacagct tggttttata aaaaacaaca aaaaggaaac aataggaggg
     7741 aaataaaatg aaaaacatca aaacaaaaat atggacgttt ttaggaactg ctatcatgct
     7801 gcttcctttc gttctcggcc taggaactgc agaggtttct gcagccgtat cacctacacc
     7861 agaaaatgtt acggttaatt tgcataaatt gaaattcact tcagcaccag aaaatcaaat
     7921 aaacaacgga acggaattga catttccgaa tagcgaaccg ttgaacggcg ttgaattcaa
     7981 cgtatatgac atcactgcta cgtactatcc aagtaaagac acagcagttc cagcagacgc
     8041 tacacccttc gcaagtgtaa cgacttctgg tgaaggatta gctaacctga ctcttccagg
     8101 taaatctgat gggaaagatg ctgtatacgt ctttgtagaa acaccaaagc ccggtgtcga
     8161 aacgtcgcca aatatcgtgt taagcttacc ctagaccctg acaaaccttt agacacgatt
     8221 cacctatatg ctaaaaatgt catcaaagaa agcaatatca aaatccagaa agctgggaac
     8281 ttttcagggg tagataagtt agcaggggcg caattccaga tcaaaaattc agagaatcaa
     8341 ttcgtgaccg gttttgatga aaacactggt gtcgctctct atgatcctgc tgagtcagcg
     8401 ttgacattca caactgatac tgaaggatcc atcagtgtca atggattaat agacggcaat
     8461 tatactttag ttgaaacaaa agcaccagat ggttatcaat taaccaatcc acaaaaagaa
     8521 actactttta tagtaaaaaa cggaaaattg caacaaacaa ccggtatcat ccatggcgca
     8581 gatgaagcca atcttgggaa tccaaacggt gtagtcaatc gggaaaatac aactacggat
     8641 aacacgatta ctgtagaaaa tgtccaaaac tacggtgatt tccacttcat caaacaagat
     8701 gtgaacacgc aagaaaaatt agccgatgca gaattttcta ttctaagagc aaaagatcca
     8761 actgttaaat taatgaaaaa aatagcaaac cttgcctcag gagaataccc atatgcttgg
     8821 tcagatgaag tcacaggttc tgactgggaa gaagtgaaat tgatttctga ttcagaaggg
     8881 aaattttcaa tcgaagggct gaaacaaggg gcatactgcc ttcaagaaac aaaagcacca
     8941 gaaggctacg cattacctaa gaatgaagct gcgtacactg aatttacagt agatgatgat
     9001 ccaacaactt ctgatgaaac aacgatcgat aaccaaccta aaggaatctt gccttcaact
     9061 ggtggtatgg ggatcatcgt attcatcttg gtaggtactg ctttagttgg aggagcagtg
     9121 atctatttca aaaaacgcca tcaagaaaca gaagcttaaa ctattggaaa caaaaatcat
     9181 acgataaata aacgatagtg attcaaatgg ggagaaaggt tgtgacaagt ggtcggtctc
     9241 ttggtattcg ctcaacaaac ggcaaaatag gaggaactat tttgccgttt gcttcgctaa
     9301 tactcaggtc aaggcgctga tttcacaacc tcttcatata catattagaa aataggtgga
     9361 catgatgaat aaaagcagaa taagaatact ggacatcttc atggcaatca ttttagtcgt
     9421 tgggatcggg atcttttctt atccttttgt agaagattca ctgaatgatt ttcttgccca
     9481 gcaaatgatc atccattatc aaaagcaagc atcgaataag aattcagagg aaataaaaaa
     9541 acagcaggaa aaaatgacaa agaaaaacca acagttggca gagcaaaacg tatcaccagg
     9601 gataggcagt ttcaaccaag cagttgatgc aaaagcatta aaagatttac ctagtaatgc
     9661 cttttttatg gaacatatgc ttggagtcat cgaaatccca aaaatcaatg tcagcttacc
     9721 tatattcgat cagacaacgg aaatattttt gcaaaaagga acgtcattat tggaaggaag
     9781 ttcttatccg actggcggga aaaacaccca tgccgttctt tctgggcatc gtggactccc
     9841 agaagcaaaa ttatttacag atttgcctaa gttgaaaaaa ggagaccagt ttttcatcca
     9901 gatcaatgga aagacattgg cgtatcaagt agaaaaaatc caagtggtct tacctgacga
     9961 agtagattct ttgggtatcc aaaaaggcag agatctcgtg actttgctta cctgcactcc
    10021 ttatatggtc aatacacatc gactgcttgt gacggggcat cgtattcctt atcaagcaaa
    10081 agaggcaaaa aaagcgatcc aaggaattga tcaatggaaa aaatggaaat tttttatttg
    10141 gtttatcggt atactacttg gatcaatcgg cttagtttgg ctactcatcg cttatttaga
    10201 ttctttggcc atcgctaagc gaaactatcc tttatctttt tatgtaaaga acacaaacgg
    10261 acgaccaatc gaaggaatgg tcttttctgt aaaaacgctc aacggcaaac actatattac
    10321 tagagaaaaa gttccatttg tgaaagcttc tgatgaatac ggactagtga gattttctga
    10381 tttaaaaggc aggaactatc gcttgcaaca tgaggagctc ctgcttaaaa tccatgtaaa
    10441 acataaacat tcgaaacagt tttcaatgaa attgaaaaaa ggaagatata aactgcgaaa
    10501 agaaaaagag gtgtactatt tgattgagaa agaatgaatg aagatgtcca aaaaagcaga
    10561 gggaacagac taaatgaaaa aagagagaag ctgacgaaat ggacgtgtaa actgctattt
    10621 ttggtttact atctggaacg ccagcttcta tttttttatg tttcagaaga attttattat
    10681 tatagtataa cttttgaatt agtttccata acatttggag caaggggtta aaccacgagc
    10741 ttgagcttcg cttaaagtgg caggggtata cgtaccattt ccacatttat gtgtatggta
    10801 ttttgaaccg gtcggtgtga catagacagt ttgttcatta ttttgctcag gagctgcggc
    10861 ttgtgcctgc tgagaagcct gttcagctgc tgcttgctgt cgcgcctgtt ctgcttgttg
    10921 ctgtgcgatt gcttcttgcc tcgcctgttc tgcttgttgc tgtgcgattg cttcttgttg
    10981 cgcttgttct gcttgttgtc gtgcaatttc ttcttgcttt ttccgttctt cttctgcttc
    11041 ttttgctttg attgtggaat caattgcagc aagacggctt gtcaactctt gtttgttttc
    11101 aggtagcgct tggatagctg ctgaagcagt tgcatagttt tcttttgtag gatttgcttc
    11161 agcttgtgac aaagcagatt ccgcttctgt cacttttttt tctagtgcag ctttttccgc
    11221 tttttgtttc tcttcttctt ttttcttttc aatttgttct ttcgatgaat ctgccgtact
    11281 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
    11341 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaacg caactaattt
    11401 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
    11461 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
    11521 tcccgtgttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
    11581 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
    11641 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
    11701 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
    11761 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
    11821 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
    11881 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaacaa
    11941 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
    12001 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
    12061 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
    12121 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
    12181 acggacgtca aagaatagtc gccgccagat agaaggaaat tagcagttga aaaagctgcc
    12241 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
    12301 gtcttattac cgcagatgca agctttttcc acacctcttt cttgtcattt acagactcaa
    12361 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
    12421 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
    12481 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataac
    12541 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
    12601 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
    12661 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
    12721 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
    12781 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
    12841 attttgcata attgaaggta gccaattcat taacccaaaa tagccggcaa tttggacagt
    12901 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
    12961 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
    13021 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
    13081 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
    13141 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
    13201 caaggacata cacgccccat attctcctcc cgctcctaca cctgcgataa agcgaaagag
    13261 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
    13321 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
    13381 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
    13441 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
    13501 ctctaaagca attcccgccg atgtcgatac taataccttt cgctgatacg ttgtcatttt
    13561 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
    13621 aaacaagact tttttatttg aaataataca caaacaaccg atatagataa tccagcttag
    13681 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
    13741 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
    13801 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
    13861 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
    13921 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
    13981 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
    14041 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
    14101 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
    14161 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
    14221 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
    14281 caactgaagg agttagaaga gtaactagga acagaactgt ttgtacgcga aaaccgcaaa
    14341 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
    14401 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
    14461 tacgatcggc tgtgtggaag ctgatagttc gaatacattg gcactattac tagaaaagtt
    14521 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
    14581 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
    14641 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
    14701 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
    14761 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
    14821 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
    14881 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catcattcaa agcgagtgac
    14941 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
    15001 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
    15061 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
    15121 ttgtattttt tcatttttaa atcaaaaaaa taaagagatt tacataactt gttaaataag
    15181 cgcttacaaa aataagaaga acggaaatga atcagctata ataaccgttg ttctggattg
    15241 taatcattac aaagaaatga agattataac tttggaggtg ttcgaaatga aagaaaaaaa
    15301 gcaaaaattc gttcaaaaaa catgcccttt attcaatggt tgggtatgat tctgctgatt
    15361 gtattagctt tattgacgat cattcctttt ttatggatgg tttctgcttc attcaaagca
    15421 aataatgaag tgttcacgat tccgattcaa tggatcccta aaacttggca tttagagaat
    15481 tatctggtta tttggtctcg tattccttta gtgacatttt ttaagaatac agctttttta
    15541 agcgttgtga tcacgttgat tcagttgttt acttctagtt ttgctgctta cgggttttct
    15601 aaactaaatt ttaaagggcg cgatatttta tttttagcat atatcggcac gattgctgtc
    15661 ccttggcagt cttatatgat cccgcagttc attatgatga gaaaagtggg tttaacagat
    15721 acgttgtggt cgttggtgct attacaagcg ttcaatgctt ttggtgtgtt cttgttgaag
    15781 caatattaca gtagtatccc agattcttta tgtgagagtg cgcggatcga tggcttatca
    15841 gagtggggaa tctatcgcaa gatcatttta cctcttacaa aaccggcgat tgccagtttg
    15901 acgatcatca cattcgttaa tacatggaac gattatatgg ggccattcat ttatttgtct
    15961 tctacggaaa ataaaacaat ccagttaggg ttgaaaatgt ttattgggct atttgatgca
    16021 gagtacgcat taatcatggc tgcctcagtt gtttctgttc ttcctgttgc ttttatattt
    16081 ttagcgatgc aaaaatattt tgtagaaggg atcgcttcat caggaatgaa ggaataggag
    16141 gtgattatga aagagcagcg ttttgacaaa aatgtttata tggaattctt ttattggttg
    16201 tatcaatcat tggcaagtag cctttgtttt tggtgtgtca atatcttgta tatagcagct
    16261 gcgttccttt tgtcgataga tggaaggaat gtcttttcct ttattttttc tttacttttt
    16321 attggtccag gttgcatcgt gatgataggt atgacagaag aattagcaaa agaaaatagt
    16381 ttttcaccag tacgtttgtt cataaagatg tttcgccaat tttggctaaa gggatttttt
    16441 tattggctat ttgcttggat agttagtgtg ataatgattt ttgattgttt cttttttata
    16501 cgtttttctt atggaaaatg gttgattccg ttgtttgttt tacttgcttg tttatcagtt
    16561 tcctttagca ccaactgctg gtattttcag gtgagaaatc cagcgtcgaa accaaatcaa
    16621 gttctacgga tagcgttcta ctatacgttg aaaaaatggt atgtgagttt actagatttt
    16681 ttactattga caagtttgtt tctcttcttt ttcgtaaagc cccaatggtg tattctacta
    16741 ggtccttcta tcgtatttgg tttgatttac ttcaacaatc gtaaattgat gagaactatg
    16801 gatctgtaga ctttataaat tgaaactttc aattaagaat gacagttttt tttatgtaaa
    16861 aactgtcatt ctttttcatt ttttgaaaca tatggctaaa caaatgaaat aaaacgtgct
    16921 atacttttgt atgaaacagg tttagaaact gtatcactta tttgtgatta tttctaacta
    16981 ataattaaaa ataaagcata aaaaggacga tgttcaatgg ctgaaaaaat gaaacaaggt
    17041 tcgaaatctg tagtagaaag tttgattaat catcatgttg attatgtatt cggtatacca
    17101 ggtgcaaaaa tcgatggtgt atttaatgaa ttagaggatc aaggtcctga attaatcgtg
    17161 actcgtcacg aacaaaatgc tgcatttatg gcacaagcaa tcggacgtat tacaggagag
    17221 ccaggagtag ttatcgcaac aagtggtcct ggtgctagta acttagctac aggtttggta
    17281 acagcgacag cagaaggtga cccagtttta gcaattgccg gacaagtaaa acgaagcgat
    17341 ctattgaagt tgacccatca aagcatggat aatgcagcac tgttccagcc aatcacaaaa
    17401 tacagtgcag aaatccaaga tccagaaaca atttcagaag tgattgccaa tgcttatcgt
    17461 atggctaaga gttcaaaaaa aggcgcgagc tttatcagta tcccgcaaga tgtagtggat
    17521 gcacctgtcc aaggtaatgt catcaagcca ttgagcgatc ctaaattagg ttctgcttct
    17581 gcagatgata tccgttacct agctgaacgc attagagaag caaaactacc ggtactatta
    17641 gtaggtatgc gaggatcaag tgaaaaagaa acgttagcca tcaggcaatt agttgaaaag
    17701 acagcattgc ctgttgtaga gacattccaa gcagctggag tgatctcacg tgaattagaa
    17761 gcgcatttct ttggtcgtgt gggtttattt agaaaccaac caggagatat gcttttgaaa
    17821 cgaagtgatc tagttattgc aattggctat gatccaatcg aatatgaagc aagaaactgg
    17881 aatgcggaaa aagatgcgcg gatcattgtg atcgatgaag cgccagctga aatcgatcct
    17941 tttatgcagc ctgaaagaga attgatcggt gatatctcag ccactcttga tttattgaca
    18001 ggttcattag agcctcaaca agtgtcagaa gatgcaaaag aatatttagc atctcttcaa
    18061 gcaaaattaa cggagcggga cattgttcaa tcaaaaggag aagcaggtat cttgcatcct
    18121 ttagaagtta ttaacacgct tcagtcaaaa gtaacagatg atatgactgt taccgtagat
    18181 gtaggtagtc attatatttg gatggcacgc cactttagaa gttatgaacc tcgtcatttg
    18241 ttattcagta atggtatgca aacgcttggt gttgcattgc catgggcgat ttctgctgca
    18301 ttggtgcgtc caaatacaca aatcgtatca gtttccggcg atgggggatt tttattctct
    18361 gcccaggatt tagaaacggc ggtacgtaaa aaattgaata tcgttcattt gatttggaat
    18421 gatggacact acaatatggt agagttccaa gaaaaaatga aatatcagcg ggcgtcaggt
    18481 gtagactttg gtccagttga ttttgtgaaa tatgcagaag cttttggcgc taaaggaatc
    18541 cgagctacaa gtgtagagga attagaaaaa gctttagaag aaggatttgc gacagaagga
    18601 cccgtaatta ttgatattcc gattgattac cgtgataatg agaaattggg agaaacaatc
    18661 ttaccagatc aattctatta aggaagtgtc gacatgactg aaaaaatctt ataccagcac
    18721 gggacattag gagctttgat ggccggcttg atggatggaa cagaaacgat tgctcatata
    18781 ttagaaaaag gaactctggg attgggaaca ttgcatggtc ttgatggtga agtcatcttt
    18841 ttagatggcg tagcttatca aggaagatca gatggctcag ttgcccaatt agatggaagt
    18901 gaacttaccc cctatgcagc aattacagat tttacgccag atactagttt ttctgtctca
    18961 aaaactgcag atggagaaca attgaaaaaa gacatattag taagagaagc cggggaaaac
    19021 ctatttttgg cagtgaagat aactggatta ttcaaaaata tgcacatccg tatcatgccg
    19081 aagcaagaaa aaccttacag aagactagca aaaatttcag agtctcaacc agaattccaa
    19141 caaagcaata tccaaggaac attagtcggg ttctttacgc ctgaattatt ccaaggagtt
    19201 gctgcagcgg gatttcacct gcattttatc gatgatacac atacatttgg cggacatgta
    19261 atggattttg aagtggctga aggaaaagtt gagattagcc gcatctcgtc ccttgtacag
    19321 cattttccag ttgatgatcc gacttttgtt cacgaaacga ttgattatgc tgatttagca
    19381 gcagaaatcc aacaagcaga atagaaaaag acagcgagat gcgttcaagg tgattaacct
    19441 gacgtatctc gtttttttga ggaggaaacc tagcgtttac aaaaaaactg agctataatc
    19501 ttctccatag atcaaaaagg agcggttttt atcatgtgga cagaaaaaca gaaaaaagat
    19561 gcatacgaaa ttttattatt acagcaacaa ggattattag aagcagaaga taactggtta
    19621 gcaaatttag caaattcatc tgcattgttg aatgagactt tgcctgagac tgtctttgca
    19681 ggatattatt tattcgatgg taatgaattg attttaggac cgttccaagg gcgagtttct
    19741 tgcacacgta tcaagatggg aaaaggagtt tgtggagaat cagctgaaaa acaagaaaca
    19801 ttgatcgttg atgatgtcaa aacccatgca aattacattt cctgcgattc agccgcacaa
    19861 tcagaaatcg tagtgccaat ggtaaaagaa ggtaatttaa tcggtgtttt agatatcgat
    19921 tgcggagtaa cgaaagggta tgatgaaatc gatcaagcat ttctagaaaa atatgtagat
    19981 cttttattag caaattttaa tataaatgct ttttcattta ttaataacta aaatcgtcta
    20041 ttgaaacaaa aaagaagaga aatcaagaat aaaaagagcc ggaaagacag tggtcatttc
    20101 tgtttttcgg tttttttatt tttttataaa atcacaagtg ttattgattt tcacaatcat
    20161 attttaatgg tattatcatt ttgaataatg aaaaaacatt aaggatggat gaaatatgaa
    20221 taacgaaact atcattttag gaaaaaaata tatttgccaa cctattggct tgaaacaaaa
    20281 agtggttgga gaagtaatca gtaaattaga aaattgtgtt gtgatctgcg tagacgaata
    20341 tcatgacgtc gatcgcaatg aaatattaga aaaatgcgga aaagtagtgg caaaatatac
    20401 tcacgtttat gagttggaaa caacagaata tctatataac tatagcaaca aatatgaaca
    20461 tactttagtt tcataaagat ttaaataaag tgccttcaaa agcgagaaga ccaagctttt
    20521 gaaggcactt tatttagttg gcagtgattg ttcatgattt ttgatttatt caaaaggttc
    20581 gcctagagaa tctgtctctg taacgaggcc tggtggagaa actcgataaa aacctatact
    20641 gccagaactg tcctattttc ggatagacaa actagtagtt ttaaaaagat catccgatcc
    20701 aatcatttca aaaaatgcaa aactgatatc aggatcattt agctcaggct tgttatcgta
    20761 aagcatttgt aaaggttgca tttagttagg agtaaacgcg aaactatcat cattatttgc
    20821 tgaagaagat gaagaagcta attcagagtt tgtttgaaat aaaggagacg tgctgacagt
    20881 ttctttaccc tgttttttgc agaggtagaa acttttatat cggaagtttc taccggagaa
    20941 tttttctcat ttgtagaaat tgttgaatcg atactattcg aaatttgcgt atctgaaggt
    21001 atgttctttg atgcagaagt cccacaagca gtaagtaaca ataaatttga taaactgata
    21061 aacagaattc ttttcattat gtacacactc cttatagtat cgtttagcat acacctatgc
    21121 tcagtattag gcaaaatcaa tgcaaaacta tcaagtcttt tatagtatct attagtctta
    21181 gtaatacata tatggagagg gtgatatccc agttcaaacc agatacattg gttaccatct
    21241 gtattaacga ttatgcctct aaacgctttt tttatgaaaa aatttataag tgaaaacgta
    21301 ggtatgatat tatctagagg taattttttt gggtatttaa atagttgcta ggaggatagc
    21361 ttttggattt ttacagtaat tttatcttaa taatagcaat attattactt ctaaatattt
    21421 ggttttttga taagagtaga aatgcaggga taggttttag aaccaaacga agtacgtctt
    21481 ctgaaaaaaa gtgggtatat tcacagacta ttttttacgg aggggttatt tcaatcagtt
    21541 tactttcttc aacactttat tcttttaatg ttatagacgt ttccatgtca aattttatat
    21601 ctattattgg aatccttatt tctgcaatta ttacacaact acttcttgtg tttgaagaga
    21661 agtcaaaaaa taattaaagc aattatgcct tttagtacag gaattattac ttcttacaaa
    21721 tgtatagcga atgtgaagcc tgctgtttta attgttctag aaaaactttt gagtgaacaa
    21781 ttattttatg ttaggatgaa ttaatttctc tagagtttat aattaaaaaa cttatttgaa
    21841 ggaacatgct taaaccataa ttgtgttttt ccttccatca gcataaaagt tcacctcaaa
    21901 aagagtgtac gaacaaaagt tctttttgta aaccttaaaa tagattttcc tgaatacgta
    21961 ttggggattt ctaaatgttt gaatgttttt ttgaatgcaa aatcaatgca aaaaattttg
    22021 tatttctcta cttttctctc cttgaccaaa ataaaaaaac gttgatttaa caacgttttt
    22081 catacatgtt tatctgtctc aaccatacat atatggagac ggcgggagtc gaacccgcgt
    22141 ccaaacacat cgccacttaa aaatctacgt tcatagtcaa ctcatttaag gattcacttt
    22201 acagcttgcc gagtgacagg cattctgtat cgctagtctg atgttctctt tttcaactta
    22261 cagacggaaa gttgaaacgt atcccactaa agttaggacc cttactcgag cacatgggcg
    22321 atgccgagag gatctacgct aactgttttt aggcagctaa agcgtaagag ttgttttcgt
    22381 ttttagcagt tatatttaac tgtaacgttt ttacgtagac gtaacctacg aaacgcaatt
    22441 caagctcgac tgtgcctgtc gaatccgtaa cgtccccgtt agctaacaag ggttacgtgt
    22501 atttttcata caaaattagt atagcatata ttgggataaa ttcaataaaa aagcgaatca
    22561 tatcccatta tttatataat aactgcccct ttcttaagaa aaaataagcg attttcttaa
    22621 gaaaagaggt agcttcaaaa aaagatctta aatcagatca aaatacttca atgcgtggac
    22681 aattccgcct tctgtatttt tctttgtgat aaaggtagaa agttctttta atccgtcata
    22741 ggcgttgccc atagcgattt tgttgtcgca agcttccaac aaggcaaaat cattcggacc
    22801 atcgccaaat ccaaaagtag gaactttttc cagctgaagt tctttttgca gacgtttgac
    22861 acctgcccct tttgagattc ctttcttcac tgtatcgatc gagtaaggac cattccgata
    22921 aaaagttagc tcaggaaaat gttcgtgata gtattcgtca cctgattgag ataagatcag
    22981 caacatattt actgtgtttt ctttaaagcc taaaggatca atttccggaa catctgaatg
    23041 aatgaagtcg taagcattga tcattgtttg gctatgaccg gtacaccaga ttttctgatc
    23101 gttatagtaa gaaagttcat gaccgttcaa ctgtacatga tcatacattt tttgacattc
    23161 ttctgaagaa aatgtttgag aatagatttc ttcgccgtct actcgaatga aagaaccatt
    23221 catggtgacc gcagaagtaa tgcccgcatc ttccatgata gagtggattt ctgcttctgt
    23281 acgtcctgta gcgatgacgg ggagaacatt gttttctttt aatgcgttca tagcttttgc
    23341 cacttcgggt gtgatttttg atttcgcatc tagtaatgtt ccatcaagat caaagaaagt
    23401 gattgcacga taatttgaca caaataaaac tcctttttta ttggaataag tttatcttaa
    23461 caaagaatga acaaaagaca aacctttcgt gaagaattcc tgaacatatt ggttcagtca
    23521 acaatttaga gtatactata agaagagaat ttaggaggga aagaatgatt tttttcagta
    23581 ttggtatctt attcttaata attggcagtc tttttcggat tctgccctca aaaggaaatc
    23641 tgccttttta tgggtatcat tcgcctcttg ccgcaaaaac agacgctcat tggcggctgg
    23701 ctcaaaagac aagcggaaac tggttttttc ttatgggctt actgatggca ttgatcggct
    23761 attatttaaa gacatctggg catacgaatt attttctgat tgaaatgctt ctgctagtat
    23821 ttccaattat gcctattttt attatgacag aaaagaaatt acagaaatat gacctggaaa
    23881 ctggagggaa cgataatgaa tattttaatg attgaagata atcaatctgt ttctgaaatg
    23941 atgcagatgt ttttcttaaa tgaaggttgg gaagcaactt tcaagtatga tggaaaagaa
    24001 gggctcgatg cctttttaga aaacccacaa cattgggata tgattacact agacctgaat
    24061 ttgcccacac tggacggtat gctagtagga agagagatcc gcaaagtttc aagtactgtg
    24121 ccgattatca tgctgacagc tagagattct gaaagcgatc aggtgatcgg cttagagatg
    24181 ggggcagatg attatgtcac aaaacctttc agtcctctga cgcttattgc tcgaatgaaa
    24241 gctttgcatc gccgtagtga gttggcagaa gcaagagaag aagcagcagt ttcagatgat
    24301 cattttgata tcgagactag tcatttcaag atgaatacga aaacacgaga agcgtatctg
    24361 gacaaccggc caattgaagg actgacgcct aaagagttcg atctattgtt tactttagca
    24421 aaaaaaccac gtcaagtatt ctctcgggag cagttgcttg aattagtctg ggattatcag
    24481 tactttggtg atgaacggac agtggacgcc catatcaaga aattacgaca aaaaatcgaa
    24541 aaggtcggac cacaagtcat ccaaactgtt tggggagtcg gctacaaatt cgatgattct
    24601 ggtgttgcct aatgcgttat ttgtatcagc aactcttagc tttttgcgga atggtcgcgt
    24661 tgatcttatt gatcgttgga gtttctttta cacaactgac aaaacaaacg attgaagaaa
    24721 acaattacca gcaactgttt ggatatgctg aatcagttga aaagacaacg caaacctatg
    24781 ccgatacgct cccgcagttt agtcaagatc aggcgtttca aaatgcattg ctgctgacag
    24841 agcaagtact gacagaacaa aatgtgaact ttatatttat cgataaaaac gaacgtgtca
    24901 tttatccgac taatacagct ttactgaatt ttaccttaac agctgatcaa tggaaaaatc
    24961 tgaggaatgg acgtcaagaa aaattcacat cgaataaaaa tattttagga caagcacagg
    25021 cgacctctta cgcacttgtg ccatttaatt tgaatcatga attctatgga gcacttgtag
    25081 ttagtcaacc tgcgcgaaat atcgataaca gcgttcgtcc aattacactg aacctgttca
    25141 aaggatttat tttttccagt atcgttgcag ttattgccag ctatgctttt gcagcattcc
    25201 aagttaagcg tatcaatcgg ttaaggaatg cgacgaaaga agtgacgaat ggtaattttg
    25261 atgtacagct ccccgtacat gacaaagatg aatttgatga gttagcagat gatttcaata
    25321 aaatgacgaa ttcattgaaa gaatctcaag cagaaattga agaacaagaa aatcgtcgaa
    25381 gacaatttat ggcagatgct tctcacgaga tgagaacacc tcttacgaca atcaacggac
    25441 ttctggaagg gttagaatac aatgcaatcc ctgaaaatca gcgagaaaac gcgattaaat
    25501 tgatgaaaaa tgaaacagag cgtctgatcc gtttggtgaa tgaaaacttg gattatgaaa
    25561 aaatccgtac gaatcaaatc agtatggtca ttaaaaaatt caatggaaca gaaacattga
    25621 aaaatattgt tgcacagtta gaagctaaag cagaagcagc aggagataca ttgacattga
    25681 aagccgttga tgatattgat gtttacgctg attatgaccg atttgtccaa atcatggtca
    25741 atattattca aaacgcgata cagtttactg aaaacggcca aatcacgatt actttagaaa
    25801 aaggctatct tgaaacgatc atcacgattg aagatacggg aattggaatg tctgaacaac
    25861 aaatgaaaag tatctgggat cgttattata aagtagatcc ttcaagaaaa aatacaaaat
    25921 atggtgaatc aggattaggt ttaccaatcg tgcagcaact tgtacgcttg cataaaggaa
    25981 aattagaagt agaaagcgaa ctaggaaaag gaacaaaatt cgctgttcgt ttgcctgacg
    26041 tagaaatcaa aaaagagtaa aaaacaagcg gtataaaaga tcatgaatcg atcttctata
    26101 ccgcttgttt ttactataaa atgattgatt tagcccgcgg gctctaaacg atcaatataa
    26161 ggactttgag caatagtagc atcatgagta aagagtaaga taccttgatt ttcttcatga
    26221 gctaaataag aaagcaagtg gagtagctct tggatctcca atacactaag ttcatcaaat
    26281 gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg catgaagtaa ttgcagcttt
    26341 actttttcaa aagacgtcaa ctcagctgcc gcacaattaa gcaaggaagg agagatatca
    26401 agtttttctt gataggccaa acgatcttgt ttgatttctt tttccggtac atccaacagg
    26461 acatggtctt taagattcag ataaggaaac atcggccaat ttttttggat gatcgcaaaa
    26521 ttatccagtt ttgcaacaag aacctctttt ttcaagtgct ccaatatttt atgctgtttt
    26581 tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa tcaaatattc aggtttcatt
    26641 acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa gtgaaaggga atagactaag
    26701 aatgagacag catccactga gcccaataag agtttgggca aatcccttaa gtatatctgt
    26761 gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc tgttgatcca tcggaattgt
    26821 aagtccgctt accgaagtca agatatcacg gatatctggc agttcagtag tctttaagac
    26881 aagaaaattg atcctttgaa gaagcgcttc ataaactgac tggaagctta cgatccacaa
    26941 taaaaccaag atacaacaaa gaaagagagg aaagaacagc tgccagaaaa taaaaaggaa
    27001 taatttggtt ttagaaagac cagtcaggcg ccaattgatc aattcctgct tgttttgcct
    27061 gactgaatag taaaagaaaa agataaaaag gaaggcaaat agaaaaaata aactgaatat
    27121 gccttttata taaaatcggc tagctaattc taaagccgga agaagctctg tagatgcaga
    27181 agcgccaata aatgaaagcc gatcttggac ttgattggct aaagccgatt gcaaatggta
    27241 aagagtcagg catgctatca ataaaaaagc agaaaagctt gaaaataaac aatagagtat
    27301 tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag cggattattt tcatactttt
    27361 cacctctata aaaagtatag aaaatgaata tgaataaagt atgaaattcc tttagtgaaa
    27421 tcacgaatta aaaccagaaa atattttgta aaaagcaaga ggttgtgaag aagctgccct
    27481 attctattac cgtagatgca agcttttgaa cactgtttat tcgaaaagag gagcagcgac
    27541 aataattttg tcgctgctcc ttttaaattt attttgtcat tcggaagaac ttgtggtcgt
    27601 tccgtgaagt tcttggtacg tttgcgtttt ataaagatca acagttgatt gtccgttgtg
    27661 ttcactaaag acactagttg acttgtcacc aagagatttt tcagcttctt ttaatttctc
    27721 taagccattc ttgtagtcat aatcactggt atcaattggg tctaatccac tattcgtata
    27781 gaaccgaaga agatcaccat tattgatttg gtcagaaaca gctaactgtt tatcaacttt
    27841 ttctttcagt gaatcaattt cattctgtac ttcctcagta ggttcagtaa tagcttcacc
    27901 agtttcgtta ctatagatcg taccaccgta gtaagtatat tttggtgtaa caaaatcacc
    27961 atcacggaaa gaaacaagct gttcatgatc tttcgagaat agatcttgtc ctaactgaat
    28021 ataatttttt gtatctacac ctagtaagtg aagtaatgta ggcaaggcat caacttgtcc
    28081 gccgtaagtg tgattgattc cgccctgtgt ttgtccaggg atatggatca tataagggac
    28141 acgctgcatt tgtgcattgt cgtagtttgt ccaagtagaa cttgtttttc caactaattc
    28201 agctagattt ttatttcttg aattcgagac accgtaatgg tcaccgtaaa ggacgatgac
    28261 tgaattatca tacaagccgc tttctttcaa ataattgaag aattcttgta cggcggtatc
    28321 taaataattg gctgtcgcaa agtaaccgtt gatcgtttca tcatttgtat tggctaacgg
    28381 gaatcctgct tcgtcattgg tgaattttga ataaggataa tggttagata cagcaatgaa
    28441 ttttgaataa aatggttgtt gcagacgttc caagtattgt acggattgtt ggaaaaatgg
    28501 tttatcatgc aacccgtatt ggaacgaatt gtcatcatta acatcataat aagaagaatc
    28561 aaagaaataa tcatatccta aattcttgta tgtctcgttt cgattccaga atgtaccagc
    28621 gttcccatgg aataccgcac tagtgtaacc ttgtgtttga cttaaaatat caggtgcagc
    28681 ttggaaagta ttttttccgc ctaattgtgt gaacaaggag ccttgattca agccaaacaa
    28741 tgaattttcc atcagcgttt ccgcatcgct tgtttttcct gcttttactt gatggaaaaa
    28801 gttgtcaaaa ctgaacgtgc tgttgctgtg gtaaagacta ttgatgaaag gtgtaacttc
    28861 atgctggaga ccattctcat ctgtcaattt ataatcgatc aggaattgct ggaagctttc
    28921 taaatggata taaatcacat tttttccttt agcgagcccg aacaaatcgt catttggtgc
    28981 tgcataatgt tcttttacat aattttctac ttctttcata tcattgggac ttgcttctgc
    29041 ccgcacttgg tttgtctggt aagtttgaac accgtcataa accatgaaag cattcaagcc
    29101 aagatatttg accaaataat cacgagagaa tgttcgagtc agcaattctg gacgatcagc
    29161 ttccgccata aaaagatttc ctgagaaaat cataactgct aatgtagaga tcgcaaaagc
    29221 cattcttgct cggatcgggc gagggtccaa cttgattttt tttgcaatca gcagaacagt
    29281 caagataata aaatcgatcc aatagatcac atcatatggg cgaaacagtc gaatggcact
    29341 ttctcccaga ccactcgcta ctttcccagc acctaaaatc gtgttgatcg taatgaagtc
    29401 cgtgaattct cgataataag tgacattcga aaaaacaagc aatgtcaaaa gaaaataaat
    29461 gaccatcatc gtgatataag caactctagg gcgtctgata taaagagcaa ttccaagtaa
    29521 taacaatgta gttgcaatag ggttgataaa caagataaag tattgaatgc cactttcgat
    29581 tccgagatgg aaatcaacta aataggcaaa aatcgttttc aaccaaagta aaacgactag
    29641 taaagaaaaa aatccgagcc tcttattcaa aaaggcaggt gtttttatac atttcaagat
    29701 aatcttcctt tctgcacttc ttaatcacag aagtgtcatg aaaccatgtt acacagctca
    29761 cttattctca tttattttac tagtatgaca atatagtgtc aattgaaacc agaagtttat
    29821 gctgtttcat taaggttttc tttaagcttc atgaaaaaat gagcagtaag acaagtccta
    29881 tgttttttgt tatacttggg aagagaaagc taaagcaggt gaaggaatga aagtaaaagt
    29941 aaaaaaaaga gcaagcaatc gattgaaaaa aggatatcca ctgatccaaa aagaagacct
    30001 tgctcatccc caaaaagaaa ccacgcaatg ggtggaatta gttgattcgc aaaaccaatt
    30061 tatcgcaaaa ggatatctag gaaagcagaa taagggaatc ggttgggtat tgagccagca
    30121 gaacatccca tttgatcaat ctttttttga agaatttttt gtgaagtcaa aagaaaaaag
    30181 aactttcttt ttttcagatg attctactac cgcttttcgt atattcaatg gagaaggaga
    30241 cggaatcggc ggtctgattg ttgattatta tgcaggttat gctgtttttt cttggtataa
    30301 cgaaacgtta tatgcacatc gtgaattatt gattcaagct tttcgtgaaa tctacccgga
    30361 aatcgtagga ggctatgaga aaattcgatt tgaatcagta aatctgcctg agagccagtt
    30421 catttacgga aaagaggcac cggaaccctt gatcgtaaaa gaaaacggag taaaatttgc
    30481 cgtttattta aatgaaggat taatgactgg gatcttttta gaccaaaaag aagtacgggg
    30541 atcattagca gaaggtttcg cttctgggaa aacagttttg aacatgttta gttataccgg
    30601 cgccttttct gttgctgccg caatgggagg ggcaatcgaa acaacaagtg tcgatctagc
    30661 aaaacgcagt ttaaaaaaaa cacaggaaca gtttgaagtg aatggtttgg atgtcgatca
    30721 gcaaaaaatc attgttatgg acgtattcga gtatttcaaa tacgccaaaa gaaaacaatt
    30781 tttatatgac atgattgtat tggatccacc aagttttgcc agaaataaga aaaaagtctt
    30841 tcgcgtagca aaagattatg gggaactggt caaggatagt ctggatattt tagcagaaga
    30901 cgggatactt attgcgtcta caaatgctgc gaatgtctcg atagaacaat tccaaacaat
    30961 gattgaagag gaattccatg ccaaaaatgt ttcttttgaa caacttgctt tatatcgtct
    31021 gccaaaagat ttccagacga cggatacctt tttagaaggc aattatctga aagtgtttgt
    31081 ttatcaaatt aaaaataacc attagaagga gcgatgcaga atggtttggg taaagggatt
    31141 gaatatagga agcggtagac caaagatttg tgcgcctctt atcggggccg tccgagagga
    31201 agtattacgt gaagcagttc ttgcacgtga agcaggggcc gatttagtcg aatggcgagt
    31261 cgatcattat cgtgaagttt tttcttttga tgaagtagca gaaacattgg ttcttatcca
    31321 tgagatgctt gatggactgc cgttattatt tacgtttcgt accttagctg aaggcggaga
    31381 acaagacata tcgatccgtc agtatcgcga cctttatgaa catgtgatac acacaggatt
    31441 agtagatatg gtcgatatcg agttgttcaa agtagaaagt ctaggaaaaa gactgataga
    31501 ggaaatcaag tctttaaaca ttcctttaat tatcagtagt catgatttta aagaaacacc
    31561 agcagaccct gtcttgcttt accgattgaa tgttatggaa cattttggag ccgatatcgg
    31621 aaaactggca gtaactccca ataatgaacg agatgttttg cggttgatgg agttgactag
    31681 acgggcaaat gcttttgtct ccatgcctat tattacgatg tccatgggaa atcttggtaa
    31741 aatttctcgt ctggctggcg gaacgacggg ttccgttatg acatttggtt ccttaagcag
    31801 cgattctact tctgctccag gacaattacc ggtcaaagca ttgaagcaaa ttattcaatt
    31861 attaaatgaa ccgttgtaga acaagtttga ggtgatattt tttggcaaag aaaaaaatag
    31921 taaaaacgaa tgcaatccgt atggtcgaac aaaaaaagat tccctataca gagcacgaat
    31981 atgagtggga cgaaagccac ctaagtgcgt catcggtcgc tgaacagctt ccagaaagcc
    32041 aatctcgtat atttaaaaca ctggttgctg tgggcaatgt gacggggcct cttgttgctg
    32101 tgataccggg agaagccgaa ttgaatctaa aaaaattagc aaaagtttca gggaataaaa
    32161 aagtagaaat gctccattta aaagatttgg aagcaacgac cggatacata cgtggtggct
    32221 gctcgcctat aggtatgaaa aaactatttc ctacttattt agatcaaatt gcagaaagct
    32281 atgaacaaat cattgtctcg gctggcagaa gagggctaca gatggagtta gcacctcaag
    32341 atatctgtgc attaacgagc ggacaatttg cagatataaa acaataaatc atacgtataa
    32401 aagaaagaag gaggccggga tgttgtttgt ccagtctcct tctttctttt ttatacatta
    32461 tgctctttgc ccggcattca ttcctgctac atgccctgta gcgaaagccg ctgtgatatt
    32521 aaagccgcca gtataaccat tgacatccag taattctccc gcaaaataca gaccatcaat
    32581 gattttactc tccaatgttt taggataaat ttcctttaga gaaacccctc cgccagttac
    32641 aaatgatttc tcaataggaa aagttttgtt gattgatagc ttaaactctt tgcatagttg
    32701 aacaaaatct tgaatttgtt tttgagaggc ttgttgtcct gtcagatgat ccatttcaag
    32761 ccgctcaaga aagaaaacta acaatcgctc aggcaatata ccatgccaag cattgaccaa
    32821 gttctttttg gttgctttac ttttttctgt caatatatga ataagctctt gtttggtttg
    32881 tgtagggaaa caatccagag aaagtgtcac tggttctccc gttttctcta attctttatt
    32941 gacaaaactt gaacaacgca gtgcagccgg accagaaata ccgaaatggg tgaaaagtaa
    33001 atccatcgta tgttcagtca atacacgacc ttttttattt aaaacgcgta aagtgatgtc
    33061 ttgtaaagaa agcccttgaa gtgttttctc ctgaatatac ggttcatccg atatcaaggg
    33121 ggactctgtc gcataaagag gcgtcaccgt gtgacccact cgcttcacga tcttgtaccc
    33181 atctccagta gaaccagtag atggataagt ccgtccacca gttgttaaga taatggcagg
    33241 agcatggaac tcttcgaatt ccgtgcgtat accgtaaatt ttatgttctt tgtggatcaa
    33301 tttctctact cgagtagaga atagaagcgt cacatctagt tctttcagac gattgagcaa
    33361 tgcttcgata atcgtttttg atttatttgt tacaggaaac atccgtccat gatcctcttc
    33421 ttttaaatgt acgccttgtg attcaaagaa gttcatgata tcgaaattat tccattgcgc
    33481 aaacgtacta tataaaaact ttccatttcc aggaatatgt gcaatcaaat catcaacagg
    33541 acgattgttc gtaacattgc accgaccacc acctgtcatc agtagttttt ttcctgcttt
    33601 tttgtttttt tcgatcaaga gtacgcgtgc gccttgttca gctgcactga tcgctgccat
    33661 catcccgctt gttccagcgc cgactacgat cacatcatac agtttttcca ttccctcacc
    33721 tcaaggaaag tgtagcatat ctgaagaaag aaaacagaaa aaaaatgttt tgagccaaaa
    33781 gagaaaatga gctaaaatga gggaagataa gcaaaggagc gtggaacgat gacaaacgca
    33841 acagaatatg ttcaagcaat tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa
    33901 tttttacaag caatcgatga attcatgccc acggttatgt cttttttgga cactcatccc
    33961 gaatatatcg aaaagaatat cttagaactt ttaacggaac cagaaagagt tattcaattt
    34021 cgagtgcctt ggcaagatga tgaaggaaac tggcgagtaa atagagggta ccgtatccaa
    34081 tacaactcag ctatcggccc ttataaaggt ggattgcgct tccatcctag tgtcaatcta
    34141 agtatcttga aatttttggc ttttgaacag attttcaaga atagtttaac cggtctgcca
    34201 atcggtggag gaaaaggcgg aagtgacttc gatcctaaag gaaaatctga taatgagatc
    34261 atgcgttttt gtcaaagttt catgctggaa ttgtcaaaac atatcggccc atcgattgat
    34321 gttcctgcgg gagatatcgg tgtaggagct agagaaatcg gctatttgtt cggcgaatat
    34381 aaacgattga aaaaatatga tactggtgtg ttgacaggta aacctttgga tttttgggga
    34441 agtaagatac gtacggaagc tactggttat ggattagtgt attatgtcaa acatctattg
    34501 aacgaagaaa aagattcatt taatcaaaaa acagtttttg tttccggaag cgggaatgta
    34561 gccatttatg caatcgaaaa agtgcaagag ctaggcggga aagttgttac atgttcagat
    34621 tctagcgggt acatctatga tccagaagga atagatgtga aactattgaa agaagtcaaa
    34681 gaaatcaata gacaacgttt gacggaatac gcaaaccaac gtccttctgc tatttatcat
    34741 gctggagaat ctgtttggac attgaaagaa aaagcggata tcgctttgcc atgtgctact
    34801 caaaatgaaa tagacgaaag cttggccgaa atcttagtag aaaatgggat caaaatcgta
    34861 gcagaaggcg ctaatatgcc atgtacagta aaagctgttt ctgttcttca tgaagcaggc
    34921 gtgtggtatt gtccaggaaa agctgctaat gctggaggag tagcagtatc tgctttagaa
    34981 atgagtcaga acgtccagcg attgacttgg gaaagagagc aagtggatga gcagctggat
    35041 caaatcatgg aaacgattta ccatacgtgt cgccaaacag cagccgagta cggcgacgaa
    35101 aaaaatctgc ttcttggagc aaatgttgct ggatttgaaa aagttgccaa agcaatggca
    35161 gcagaaggtt tagtttgaaa aatgaaactt tcttggaaat attgtcagca ttttgtttat
    35221 ttgcttgaaa aataataaaa aaaaacgtaa agtattacct gtagagtaaa cctataagga
    35281 ggagaacaaa tggcacacat tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa
    35341 caagagttag ggtacatgca aagccaagta acagcagccc acaacgaatt acgggaaggt
    35401 actggcgcag gaagcgactt ccgcggctgg atcgatttac caacgaacta tgacaaagaa
    35461 gaatttgcac gtatcaaaga agcagctaaa aaaatccaat ctgattctga agtattagtt
    35521 gttatcggta tcggtggttc ttatttaggc gctcgtgcag cgatcgactt cttgaatcac
    35581 acattcttca atttattaga tagcaacaca agaaaagcac cacaaatctt ctttgccgga
    35641 aattcaatca gctcaacata tattgctgac ttgatcgaag taatcggtga tcgcgacttt
    35701 tcagtaaacg ttatttcaaa atctggaaca actactgaac cagcgattgc gttccgtgta
    35761 ttcaaagagc ttcttgttaa gaaatatgga caagaagaag caaacaaacg tatctacgct
    35821 acaacagata aagcaaaagg tgcagttaaa gtagaagcag atgcagaagg ttgggaaact
    35881 ttcgtgattc ctgatgacat cggcggacgt ttctctgtat taactgctgt tggtttgtta
    35941 ccaatcgctg ttagtggtgc agatatcgat gcattgatgc aaggtgccgc tgatgcaagc
    36001 aaagcatact caagcgacaa actagaagaa aacgaagctt accaatacgc agcaatgcgc
    36061 aacattcttt accgtaaagg caaagtaact gagttgttga tcaactatga accaggaatg
    36121 caatacttct cagaatggtg gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa
    36181 ggcatctatc cttcaagcgc aaacttctca actgatttgc actctttagg acaattcatc
    36241 caagaaggac gtcgcaatat ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca
    36301 atcacaattc ctgaacaagc agaagattta gacggtctag gctacttgca aggaaaagaa
    36361 gtggacttcg taaatacaaa agcatttgaa ggaacattgc ttgctcatac agacggtgac
    36421 gtgcctaact tgctagtcaa aattccagaa atggatgctt atacattggg ttatacaatg
    36481 tacttctttg aaattgctgt aggtatttca ggttacttga atggaatcaa cccattcgac
    36541 caaccaggtg tagaagctta caagaaaaac atgttcgcac ttctaggcaa accaggcttt
    36601 gaagatcttg ctaaagaatt gaacgaacgc ctgtaagata agggtttttc gctagattta
    36661 aataatcagt gatagaaaca tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact
    36721 caaacatact gcatcgttta ttcagaataa ggggctgtga caagacgttt gtcacagtcc
    36781 cttgtttgta tagaaatcga ttgattgcct tgctctttag tttgttttag gactttaaga
    36841 acagtgaggc tgacttcgaa ggttaattct gtttttttgt tttctagaat cagtaagcaa
    36901 aaaagcactc acttcgagtg cttttttgcg atatattctt agcttaagat aggcctaatt
    36961 tttcttttac agatgagggt accttatcgc tggtaattac ttttttctct atgatccgct
    37021 tttcgcttgc tttcaactgc aagatcgttc cttcggggta agcagaacca ttggcagagt
    37081 caccagaagt aatgacatcg aaatctaact gtcttttttc accgttttca gcataagcat
    37141 ctacgttgta tttaaaacct cttccaatta cttccccatt atctgctttg atatcagttt
    37201 ctgcaggcat tggagctttg actaccgcat aataatcttt acctacataa gtagactgat
    37261 agtagtccca acctttccat cctataaaag caatcaatat gataacgatc aaccctaaaa
    37321 tttttttcat gaaagatctc cttttttatt tttattatac ccgtggtgct agttaatttt
    37381 aatatcctaa agaatatctc aaagttcctt caaaacgtcg ttgtgctcga cttaatagaa
    37441 cactgtaagc cagtaatatg ctttcaaatc tactctctag ccctttaaca gaacgtgagt
    37501 tgaaattttg acaacctaat ttttctaagg aagaaaaaac agtctcaact g
//
LOCUS       DAJSBN010000027        32178 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJSBN010000027 DAJSBN010000000
VERSION     DAJSBN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.027"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(407..766)
                     /locus_tag="ONT86_001482"
     CDS             complement(407..766)
                     /locus_tag="ONT86_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR2352283.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="ONT86_001483"
     CDS             complement(796..1137)
                     /locus_tag="ONT86_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352284.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="ONT86_001484"
     CDS             complement(1134..1808)
                     /locus_tag="ONT86_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR2352285.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ONT86_001485"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ONT86_001485"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR2352286.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="ONT86_001486"
     CDS             complement(4322..5512)
                     /locus_tag="ONT86_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2352287.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="ONT86_001487"
     CDS             complement(5533..6060)
                     /locus_tag="ONT86_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCR2352288.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="ONT86_001488"
     CDS             complement(6107..8896)
                     /locus_tag="ONT86_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR2352289.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="ONT86_001489"
     CDS             complement(9043..9243)
                     /locus_tag="ONT86_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2352290.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="ONT86_001490"
     CDS             complement(9696..10016)
                     /locus_tag="ONT86_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352291.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="ONT86_001491"
     CDS             complement(10519..11637)
                     /locus_tag="ONT86_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352292.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="ONT86_001492"
     CDS             12108..13415
                     /locus_tag="ONT86_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCR2352293.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="ONT86_001493"
     CDS             13536..14735
                     /locus_tag="ONT86_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR2352294.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="ONT86_001494"
     CDS             complement(14762..16030)
                     /locus_tag="ONT86_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCR2352295.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="ONT86_001495"
     CDS             complement(16200..16841)
                     /locus_tag="ONT86_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HCR2352296.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="ONT86_001496"
     CDS             complement(17250..17753)
                     /locus_tag="ONT86_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2352297.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="ONT86_001497"
     CDS             complement(17876..18640)
                     /locus_tag="ONT86_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCR2352298.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="ONT86_001498"
     CDS             18748..19023
                     /locus_tag="ONT86_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR2352299.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="ONT86_001499"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="ONT86_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR2352300.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="ONT86_001500"
     CDS             complement(20579..20980)
                     /locus_tag="ONT86_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR2352301.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="ONT86_001501"
     CDS             complement(20994..21803)
                     /locus_tag="ONT86_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2352302.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFVALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="ONT86_001502"
     CDS             complement(21790..22680)
                     /locus_tag="ONT86_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2352303.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="ONT86_001503"
     CDS             complement(22693..23172)
                     /locus_tag="ONT86_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2352304.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVATNTIEK
                     SGLKLATPAGVKLSILPIEKAATNILAGKYDSQRVLIVAKKPDRILSLVEKGVPIKEV
                     NVGNMSQTNETRSITKSINVVDADIEVFKKLDQLGIHLIAQMVPNDKAEDFMNLLIK"
     gene            complement(23348..24289)
                     /locus_tag="ONT86_001504"
     CDS             complement(23348..24289)
                     /locus_tag="ONT86_001504"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HCR2352305.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ONT86_001505"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ONT86_001505"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCR2352306.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="ONT86_001506"
     CDS             complement(25274..26470)
                     /locus_tag="ONT86_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2352307.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="ONT86_001507"
     CDS             complement(26488..27216)
                     /locus_tag="ONT86_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2352308.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ONT86_001508"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ONT86_001508"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCR2352309.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="ONT86_001509"
     CDS             complement(28628..29086)
                     /locus_tag="ONT86_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2352310.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="ONT86_001510"
     CDS             complement(29083..30546)
                     /locus_tag="ONT86_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2352311.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ONT86_001511"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ONT86_001511"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR2352312.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="ONT86_001512"
     CDS             31602..>32178
                     /locus_tag="ONT86_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2352313.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9385 a         6920 c         5511 g        10362 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcaacaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttgtcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DAJSBN010000028        31560 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJSBN010000028 DAJSBN010000000
VERSION     DAJSBN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.028"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            49..510
                     /gene="mscL"
                     /locus_tag="ONT86_001513"
     CDS             49..510
                     /gene="mscL"
                     /locus_tag="ONT86_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR2352314.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(570..1016)
                     /locus_tag="ONT86_001514"
     CDS             complement(570..1016)
                     /locus_tag="ONT86_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2352315.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(1109..1354)
                     /locus_tag="ONT86_001515"
     CDS             complement(1109..1354)
                     /locus_tag="ONT86_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCR2352316.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(1470..2048)
                     /locus_tag="ONT86_001516"
     CDS             complement(1470..2048)
                     /locus_tag="ONT86_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2352317.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(2125..2655)
                     /locus_tag="ONT86_001517"
     CDS             complement(2125..2655)
                     /locus_tag="ONT86_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCR2352318.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(2659..3519)
                     /gene="rlmB"
                     /locus_tag="ONT86_001518"
     CDS             complement(2659..3519)
                     /gene="rlmB"
                     /locus_tag="ONT86_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR2352319.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(3512..3907)
                     /locus_tag="ONT86_001519"
     CDS             complement(3512..3907)
                     /locus_tag="ONT86_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCR2352320.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(3904..5316)
                     /locus_tag="ONT86_001520"
     CDS             complement(3904..5316)
                     /locus_tag="ONT86_001520"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR2352321.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(5313..5849)
                     /gene="cysE"
                     /locus_tag="ONT86_001521"
     CDS             complement(5313..5849)
                     /gene="cysE"
                     /locus_tag="ONT86_001521"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR2352322.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(5935..6174)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6171..7631)
                     /locus_tag="ONT86_001522"
     CDS             complement(6171..7631)
                     /locus_tag="ONT86_001522"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR2352323.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(7801..8958)
                     /locus_tag="ONT86_001523"
     CDS             complement(7801..8958)
                     /locus_tag="ONT86_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCR2352324.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(8992..10362)
                     /gene="radA"
                     /locus_tag="ONT86_001524"
     CDS             complement(8992..10362)
                     /gene="radA"
                     /locus_tag="ONT86_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR2352325.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(10425..10952)
                     /locus_tag="ONT86_001525"
     CDS             complement(10425..10952)
                     /locus_tag="ONT86_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR2352326.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            11182..11988
                     /gene="proB"
                     /locus_tag="ONT86_001526"
     CDS             11182..11988
                     /gene="proB"
                     /locus_tag="ONT86_001526"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR2352327.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            11985..13232
                     /locus_tag="ONT86_001527"
     CDS             11985..13232
                     /locus_tag="ONT86_001527"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR2352328.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            13308..13652
                     /locus_tag="ONT86_001528"
     CDS             13308..13652
                     /locus_tag="ONT86_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352329.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(13769..14095)
                     /locus_tag="ONT86_001529"
     CDS             complement(13769..14095)
                     /locus_tag="ONT86_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352330.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(14710..15912)
                     /locus_tag="ONT86_001530"
     CDS             complement(14710..15912)
                     /locus_tag="ONT86_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2352331.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            16029..16544
                     /locus_tag="ONT86_001531"
     CDS             16029..16544
                     /locus_tag="ONT86_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2352332.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(16743..17189)
                     /locus_tag="ONT86_001532"
     CDS             complement(16743..17189)
                     /locus_tag="ONT86_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR2352333.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(17236..18714)
                     /gene="msr(C)"
                     /locus_tag="ONT86_001533"
     CDS             complement(17236..18714)
                     /gene="msr(C)"
                     /locus_tag="ONT86_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR2352334.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(19068..19697)
                     /locus_tag="ONT86_001534"
     CDS             complement(19068..19697)
                     /locus_tag="ONT86_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR2352335.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(19681..20145)
                     /locus_tag="ONT86_001535"
     CDS             complement(19681..20145)
                     /locus_tag="ONT86_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2352336.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(20632..21900)
                     /locus_tag="ONT86_001536"
     CDS             complement(20632..21900)
                     /locus_tag="ONT86_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR2352337.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(22131..22868)
                     /locus_tag="ONT86_001537"
     CDS             complement(22131..22868)
                     /locus_tag="ONT86_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2352338.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(22935..23504)
                     /locus_tag="ONT86_001538"
     CDS             complement(22935..23504)
                     /locus_tag="ONT86_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR2352339.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            23632..24504
                     /locus_tag="ONT86_001539"
     CDS             23632..24504
                     /locus_tag="ONT86_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2352340.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            24846..25718
                     /locus_tag="ONT86_001540"
     CDS             24846..25718
                     /locus_tag="ONT86_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2352341.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            25715..27673
                     /locus_tag="ONT86_001541"
     CDS             25715..27673
                     /locus_tag="ONT86_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2352342.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(28005..28856)
                     /locus_tag="ONT86_001542"
     CDS             complement(28005..28856)
                     /locus_tag="ONT86_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2352343.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            28979..29446
                     /locus_tag="ONT86_001543"
     CDS             28979..29446
                     /locus_tag="ONT86_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCR2352344.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(29709..30191)
                     /locus_tag="ONT86_001544"
     CDS             complement(29709..30191)
                     /locus_tag="ONT86_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCR2352345.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(30291..31154)
                     /locus_tag="ONT86_001545"
     CDS             complement(30291..31154)
                     /locus_tag="ONT86_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR2352346.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         9033 a         6670 c         5709 g        10148 t
ORIGIN      
        1 tttatttttc gatatactaa agacaaataa aagaagggag ggtcattcat gatcaaagaa
       61 ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg ctgtcggcgt tgttattggt
      121 agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct tgatcacacc actaataggt
      181 tggtttgtcg caatgatcac aggtacttct gatttagaag gagcactttc aattcttgac
      241 tggtcgccga caaaaggcgt cacatttgcg tttggtaacg ttattagtgc tatcattaca
      301 tttctgatca ctggttttgt cttattctta gttgtcaaag cggcaaaccg tgcgaagaat
      361 ttacgctcca aagaagagca agtggaagtg gaagaaactc ctactgcaga agattatctt
      421 agtgacatac gcgatcttct tgccaaacaa gtggcagaag acaatacaca tccagtagag
      481 caaacaaatc tttccgatac ttctaaataa taaagaatct aaaaaaaaag aaagccgatt
      541 ttcagtgtcg gctttctttt tttagattct taacttgcta cttgttcctt tttcatgtcg
      601 atatgtggaa tatcatcttc caaatacaca tctgagattg ccttgaatcc aaacgactca
      661 taaaaagctt gtaggtattc ttgtgcgccg atcacgattg gtttttctgg aaaatttttc
      721 tggatcatgt taatcgtttc ttgaacaatc tttcttccta aaccatcttt acggttgtcc
      781 tgatgaacaa ggacacgtcc aaaatggata ctctccgctt cttcatatac tcgcgtgtag
      841 gcaaggatct tcccatcagt atcttgaaaa aacgtatgcc aagcttctcc atcaatgtca
      901 tcaacttctt gataaggaca ctgttgttcc acaacaaaaa ctgcgattcg ttctttgacg
      961 atcgcgtaaa actctgctgt tgacaattct tcaaatttct ttacctgata ttccactaag
     1021 caaacgcccc ctgctttttt attcttagca taccgattcg cacacaaaaa agcaaacgaa
     1081 ccttacgaat ctcaggctcg tttgcttttt aaactgcttc agttaaaaat tcgatctcaa
     1141 ctgtccgagt aagtacatct gaatagctgt aagatactcg ttcaaaagaa ttttcttcag
     1201 gatccaaatc aacaacgaat actgaaggat aggtttctgt taaaacgccg cggcgttctg
     1261 tttgacgctt tcttcctgtc tgagcaacca acatgattgg gctgccgata cgaccttcta
     1321 aatctttctt gattgaagct aaagttgttg gcatacagtt cacctctact atcaactata
     1381 ccacatattt ttaaaatttt caagtttacc ataaaaaaaa aaagaaatgc aataaaaata
     1441 aataagaagc tcattaagat ttttctaaat tattcctcat tataaaattt caagtgtttt
     1501 tttattttga gttttactct cccgtaagcg ctcattactt gcttgtgatc cattgctaaa
     1561 cattcagcca tgtctacaaa atcaacgcct tgcatataat aatgaaaaat tttagcttct
     1621 atttttgaaa actggatttc gcaattttcg attgcttctt ttaataaaag atgctgaatt
     1681 gctaaaggtt cttgcaatgt gatctctgct aaaaagtctg gtccttctgt ctctattttt
     1741 tggtcgattg agacttcatc tttttgatca cgacgcttca gcgcgttctg cttcctcaac
     1801 agagaataga tttgattgct tataacgctt ttataataac ttccaaagct aacacctttg
     1861 cttttttcgt aagttctcaa tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc
     1921 tcagaaacat aatttcttaa ataataggtg tttttcaaac gtagtaaaag tggaaaatat
     1981 ttttgataca gggcagcaaa tgcttcttcg tcaccattac gtgcagcgcg caataactcc
     2041 tcagacataa aataattcct ccataaattc gtttatggag gaatcttatc atcaatgatt
     2101 catcgaactg attagaacga tatgttattt attttttctc attaaatcgt ccaatttttg
     2161 ggacaatttt tttaactgct cttcattcca tggagaatta cgtcggaaat caataaaatg
     2221 catatcttta ttttcttggg ctattgtttt ttccgttttt tttatcgtct tatataattc
     2281 ataagcagaa gttcgtaatg ctccttggga aaagatcatc cactgttctg ctaaatcgct
     2341 tgtcgccact gtcacttgtg tcaaacggtc attcagctga ccagcgattc tctcgatata
     2401 actgtcagcc gtttcgtctt ctttcgtaaa aacaacatcc aactggtatt tcttataatt
     2461 ttgttggatg cccggaacta gttgggcatc gaagacaacg atgacttcca ttccttcata
     2521 tttcgcataa tttgataaac gatgtaaaag agcttctctt gcatccgcca ttttgtcctg
     2581 ctttttaagc tggacaagtt ccggccacga accaatcatg ttgtagccgt ctacaatcaa
     2641 cagctgtttt ttcatggtct actccccctg acgttgggaa aaagcctgat acataagcaa
     2701 accagcagca acactggcat ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa
     2761 ttcatccatt tcttttttca atcctggact cattcctttg ccttcattac caatgactaa
     2821 agcaattgat cctttggcat tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa
     2881 tacccaaaat cctgtttctt tgagctgttg cacagcttgt accaaattcg tcacgcgtgc
     2941 aacagggaca tgttcgactg ccccagtgga agtctttgtt acgataggtg tgattccaac
     3001 tgctcggtgt tttggtatga tcacaccatc gacgccactt gcgtctgctg tccgtaaaat
     3061 cgacccaaaa ttgtgtggat cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt
     3121 tttctctttt gttcgttcaa tcagttggtc aagggataaa tattcataag gtgtaatcgc
     3181 aagaacgact ccttgatgca cgccgtgatc gctcaaagtt tctaattttt gtttaggaac
     3241 ccatttgact ggcacagctt gttcacgtgc gatttctttc agttcttcta ctttttctcc
     3301 tcgtgaatct tcttgaagaa agagtttatt ccctcgccct tgttgtagcg cttctactgc
     3361 ggcatggtgc ccaaagacga aattatcttc taatgcttct tcattttgat cagaaactga
     3421 ctgatttcgt ccagtttgct ttttatttga atatctattt tgttttttgt cttcaaatcc
     3481 acgtttcgat tgatgtttgt ttgttctttc tttacccatt tttttcacca actttctgaa
     3541 tacaccagcg gatcaattct tccatccgct cggtttgttt tgtcaaatgc agatagccca
     3601 tgagcgcttc gaaccctgtt gcgacccgat acgtcgtaac atcagcattt tttgcgctcg
     3661 tatggctttt agcatttctt cctctgcgat aataaagttc ttcttcttct gtcaaaagat
     3721 gttcttccag catttgctga atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg
     3781 ccgcacgatg cagcttattg ggttttgttt ctccttgatc aatcaggtgg tcacgaacat
     3841 agatttcgta aatcgcatct ccaacataag ccaaggctaa accgttcaat tgtgtgtaat
     3901 ctctcatgct tctcttctcc atcgggttcc ttgcggtgta tcttctaata agatcccttt
     3961 ttctttcaac aaatcacgaa tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg
     4021 atttcgttct tcgatcaatt gttcaatctc ttcatctaat aactcttctg ctgtaaataa
     4081 aataccgaag atcgaaagcc attgcgagaa taaagcttct ccttgtgcca agacagcggc
     4141 agaaactttc ggctggtcta gataagtgtt cagccactta gcaagctcat agaccactgt
     4201 gatcccattt gctgcattaa aatcatcgtc catttcttcg atgaatcgct gttctaaatt
     4261 ttctaacaaa gcgattttat ccgcatcatc tgccaaattc tctgaggcag tttcttggcg
     4321 gaatttcaaa ttgtcaaacg tattcttcag tcgttgtaaa ttggtggctg cttctttcat
     4381 cgtcgtttca ctgtagcgga tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa
     4441 gacttgtgga tcaacgtttt tgatcatttc atgtacagtg atgaaattcc ctagagattt
     4501 actcattttt tcatcgtctt cgccaatcgt tacatagcca ttgtgcatcc agtaattagc
     4561 gaattttttc cctgttttcg cttcactttg tgcgatttca ttttcatgat gaggaaattc
     4621 taaatcttgc ccgccaccat ggatatcgat cgtttctcct aaatgttttg ttgccataac
     4681 ggaacattca atatgccaac cgggacgtcc tttgccccat ggtgaatccc aagaaatctc
     4741 gccaggtttt gcttgtttcc ataaagcgaa atctaatggg tcttcttttt gctgctgttc
     4801 ttcccctgta cgctgacttg ctccgacttc cagttcatcg atggattggt cacttaattt
     4861 cccataattt tcaaatttac gggtacggta atacacatca cctgaagatt cataagcata
     4921 gcctttttca accaacactt gaataaatgc taaaatatct ggcatatgat ccatgactct
     4981 tggatgcagt gtagcaggtt cgacattcaa cgctttcgta tcttcttcaa atgctcggat
     5041 gaaacgatca gcaacttctg gtgctgtaat acctaattcg tttgctgtac ggatgatttt
     5101 atcgtctaca tccgtgaaat tcgatacgaa atcgacttta tagcctcgat attcaaaata
     5161 gcgtcgaatt gtatcgaaag caatggtgct acgagcattt ccgatatgga tataattata
     5221 cacagtcggt ccgcagacat acataccgac atgtcccttt tgtataggga tgaattcttc
     5281 tttttttctg gtcaatgtat tgtagatttt tatcattctg atctcctcac tttttctcct
     5341 ttgattcgaa cgacttttgc tggtactcct accgctgtag cgtcgtctgg aacatctttc
     5401 aacacaacgg cacctgcacc gattttggcg ttttttccga tcgttaccgg tcctaatatc
     5461 tgcgcattcg cagagagcat ggctccttgt ttaacagttg ggtgtctttt acccgtttct
     5521 tttcctgtgc cgcctaatgt tacgccatgg aataaaatca catcgtcctc gatttctgcg
     5581 gtttcgccaa tgacgattcc cattccatgg tcgatgaaca cgccagttcc aatcgttgct
     5641 cctggatgga tctcaacacc agttaggaaa cgccagaatt gagcattgat cttcgccaat
     5701 aaaaagagcc ggtgctgata taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa
     5761 ccagggtatg tcaaaacgat ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct
     5821 gcatcaatcg ctcgggtaaa ccaactcatg actttccact cctttcgtaa gaattttatg
     5881 taggaaatca tcttcttata aagaaaagaa gcgcctttta aataagtttc cttatcaaaa
     5941 gacgctttcc gcgtggttcc acttttcttc ataatcaatg attatctctg acagctaacg
     6001 cctgttatac gttcttggct actttgtttc ctataaaaca gtttttcctg ttttctgtgt
     6061 ttcgccaaga ccactcccag aggcatttcg gcaaccattc attgatacct tgcaccaccc
     6121 ggtatctctc ttgtaatgat cagctgctta cttttttctg ttcatcgtgt ttagttcagt
     6181 acttgattta aatgtgctaa tgatttttca cgaccaagca attcgatcgt ttcaggcaat
     6241 tctggaccat gcatttgtcc tgatacagct acacggattg gcatgaaaag attttttcct
     6301 tttacgccag tttctttttg aacagctttg atggcagctt tgacagttgg agcatctact
     6361 acttccattt gttctagctg tgctttgaat gcgtggagaa cagtaggaac tgtttcacca
     6421 gcaagtactt cttttgctgc ttcattcaat acaggatgtt cattgaaaaa tagcgtagac
     6481 agatcaacga tttctgcagc atagctcatt tgaggttgat acaggctaac gatcttcttc
     6541 acccattcga ttgtcttttc atctgggttt tctgctacac gtccatcagc gattagataa
     6601 ggcagacaca tgtcagtaaa tgcatctaga tccattgctt tgatgtaatg gttgtttacc
     6661 cattgcagtt ttttcgcatc gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac
     6721 attttgatca attcttggtg ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat
     6781 aatgcaataa agttgaacat tgcatctggt agatagccta attcgcgata ttgttcgata
     6841 aattgcaaga ttgattcgtc acgtttgctt agttttttac ctgtttcact gttgatgatc
     6901 aatgtcatat gtccgaaggt tggtggtgtc cagccaaatg cttcatagat catcagttgt
     6961 tttggtgtat tggcaatatg gtcatctcca cgtaaaacgt gagtgatttt catcaaatga
     7021 tcatcgaccg caacagcaaa attataagtt ggcataccat cgcgtttttg gatgacaaag
     7081 tcgccaccga tattatcgga ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg
     7141 tattctgtat tgcgaggcac acggaaacgt acaacagatt ctagaccttg tgcttctttt
     7201 tctgcttgtt cttcaggtgt caagttcgca catgttcctg cgtaatgagg catttctcct
     7261 cttgcgcgtt gtgcttcccg ttctgcttct aattcttctt ctgtgcaata gcatttgtat
     7321 gctcgattgc tagcaagcag ctgatcaatc agcggttgat agatttcttt acgttcagat
     7381 tgacggtaag ggccgtattc tccaggattt tcaggagatt catcccaatt cattcccaac
     7441 caagcaaggt tttccaactg gcttttttcg ccatcttcga tattacgttt ttggtcagta
     7501 tcctcaatac gaataataaa gtctccatca ttgtggcggg caaacaaata attgaataat
     7561 gcagttcttg catttccaat atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt
     7621 actttcgtca ttttcttcct cttctttcaa actagacttt gactaacagc aaaaattccg
     7681 tcagttacgg cttctcaaat atcatttatt taaagccact cagcacaaat tttacctttt
     7741 ctaaggtaaa tagtaaagcc aactggttga atttccttac cagttggctg aatttctttt
     7801 ttattttgat tgtttagtct ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc
     7861 atgtgcaggt ttagcaaaga tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac
     7921 gacttcgata cgttcattca tgtaatgctg tccatcttct acgacaacca tcgtgccgtc
     7981 atcaaggtag gctactcctt gttggcgttc tgtcccggct tttactacta gaacattcat
     8041 cgtttcacca gggatgacga ctggttttac ggcatttgcc aacgcattga tattaagaac
     8101 aggtacattt tggaattcag agactttatt caagttatag tcattggtta caatgacacc
     8161 atctaaaagc ttagccaatt tgatcaattt gctgtctact tcactgatat cttcaaagtc
     8221 tccatcgtac atttcgacag atataccctc ttctttttgc aaggcattta ggatatctaa
     8281 tccgcgacga ccacgcacac gtttcaaact atcccctgag tccgcgatgt attgtagttc
     8341 gtaaagaaca aaattaggga tcatcaaggt tccttctaag aatccagtct ttgtaatatc
     8401 ataaatccga ccgtcaatga tcacgcttgt atccaagatt ttatatttat ggaaattttc
     8461 tcctgatttt cgttctaaca gttgactctc aacttgacta gctgtttctt gctcgatttt
     8521 tttgcttcgt gaaccaaaaa ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa
     8581 tcccagatag cctaagatga tcatcagtaa aatcggcaat acactattga cgaatggaat
     8641 ttccatattg taaagagggg tcgagatcac aacacctaaa gtcaatccga tgatcgcacc
     8701 gatcgcaccg aagagaagat aagtcaagct gacctcactt agtttttgtt ctacttgttt
     8761 gataatagct gagatatgtt tggccaacag caaagataat aaaaagaaaa taagtgcgcc
     8821 taacaggctg ttggtaaatg tattattcag ccatccgttt gactgttgac cgattgcctg
     8881 ccaagctgag ggtaaaaatg aaatacccaa gcttgcgccg acaatgatca tcagcaaagt
     8941 aatgacacgt ttttgcatcg tttttcctcc gtttcgacaa tcatttatta gttagcggaa
     9001 cacacgcttc aacgtttcag ccaaagttgc tactccaact atctcgattc cttttggcgc
     9061 ttcccagtta cctaagttgt ttttaggtag gtatactttt gtgaagccga gtttttggac
     9121 ttctttgact cgttgttcaa tatgactgac acgtctgatt tctccagtca gtccaatttc
     9181 accgatgaaa cattccgttg gctgtgttcc tttttcttta tagcttgaag caatactgac
     9241 agcgatagct aaatcaatcg ctggttcatt taattttacc ccacctgcag cttttaagta
     9301 agcatcttga ttctgaagaa gcaacccagc tcttttttcc agcactgcca tgatcagaga
     9361 tacccgattg aaatcaagac ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt
     9421 gactaatgct tggatctcta ccaaaattgg acgtgtccct tccattgcca caacgatagc
     9481 agagccagtt gcaccgtcta ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc
     9541 ttccaatcca tgctcatgca tttcaaaaat cccaatctca ttcgttgagc caaatcggtt
     9601 ttttactgct cgcaaaatcc gaaaactatg atgttgttca ccttcaaaat ataacaccgt
     9661 atccaccata tgttctaaca ttcttgggcc ggcgatcgag ccttctttgg taacatgacc
     9721 gacgataaaa atggctatcc catttgtttt agcggttttg agtagttctg cagtggtttc
     9781 tcttacttgg ctgacacttc ctgccacact tgtgatgtct ggctgcgtca tcgtttggat
     9841 ggaatcaatg ataacataat caggggagat atgttcgatg gcacgactga tttcgttcat
     9901 atctgtttca gcgtatagat aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa
     9961 ttttatctgc tctgcgcttt cttcgccgga tacatataag acttttccgc ctatggctgc
    10021 taattgctga gaaacttgta acagcaaagt ggatttcccg atacctggat cgccaccaat
    10081 caagaccata gaccctggca ccacacctcc gcctaacaca cggttcagtt cttctaattt
    10141 tgttttgatc cgaggttctt ttttagggac gacgtctgcg atttttgttg gttttgcttt
    10201 ttctcctgtc aaacttgtcc gagttctgcg gtccgttgta tcttgttcga tttcttcgac
    10261 catcgtattc cattttccgc aatttgggca gcgtcccaag aacttaggcg acacataccc
    10321 gcaattttgg caaacaaatt gtacttttga tttttttgcc attgtaccgc ctccttttat
    10381 cctaatcatg agacattaaa tcaagcttaa gtatctttaa cgttttattt actggaagaa
    10441 ccaaatccgc cttgacgttg agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa
    10501 aagatccctt ggccgatacg ctcgcctttt ttgatcacta catccttgaa accaaagttg
    10561 gtgaattgga acatgatatg gccttcattt gcctcattat tgtaataatc actgtcaatc
    10621 acacctactc cgtttgtcaa cactaaaaac cgcttcaacg gattgcttga acggttagca
    10681 agctgcaaaa attcatcttc tcccatgtaa gctttgattc cagtaggcac gagggttggt
    10741 ttcaattgtt tttcattttc tgatacatct tttttcaatc catctgcgac tgtttccaac
    10801 ccctttttcc agcctgcttt ccagatactt ggaatcacga cctcttctgc tgcttcaaaa
    10861 tcatagccag cagcatgatg tgtagcgcgc tgcggtagat tgatattttg atctttatat
    10921 ttagaaataa cttcaaatcc tctttttctc accggttttc ctccctaaat aaactactcc
    10981 tattctaaca tatccgaggc tgtaaaagac tttctttaca aattcttcaa gaaattctgc
    11041 tatcttaaag taaaccatta attcgttctt tttccaaacg actgcaagtt gtacaaaaaa
    11101 actatttttt acatgacttt actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg
    11161 gatatttgat tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc
    11221 aaagtaggaa caagtacatt gatttatccc aatggaaata tcaatcttgc ggcaatcgat
    11281 cagttagctt ttgtcctttc tgatcttaga aatcggggaa aggaagtgat cttagtctca
    11341 tcaggagctg taggcgttgg tatgcataaa ttacatttgt cagaacgtcc gactttgatt
    11401 cctgaacagc aagctgcggc ggctgttggt caagccgaac tgatgaatat ctataatcaa
    11461 agattcagtg tatataatca acagacggca cagatcctcg tgacaagaga cgtggtagat
    11521 tatcctgaaa gtcgtaaaaa tgtgaggaat acattggaac agcttttgat tatggacacg
    11581 atccctatcg tgaacgaaaa cgatactgta gcagtggatg aattagatca tctgacaaaa
    11641 tttggcgata atgatcagct gtcagccatc atggcaaaac ttgtcgcggc agaccttttg
    11701 atcgtccttt ctgatatcga cgggttttat tcagataatc cgactacaaa tcccgaggct
    11761 cagatgtatc gctatatcga taaaatcgat gaaaagattc tggcacaagc aggtggtgct
    11821 ggtagcacat ttggaacagg cggtatgcag agtaaattaa aagccgcttc taggatattt
    11881 gaagcaaaca gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat ttttgatatt
    11941 cttgcaggaa aagaagtagg tactttattt aaggaggact tgaaatgact gacttgatgc
    12001 aattaggaca acaggcaaaa gatagcgcgc aacaactagc acttatgcct acaaaccgca
    12061 aaaatgagct acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg
    12121 aagcaaatca taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag
    12181 accgcttgca gctgacacct gagcgaatca atgccatggc tacagagatc gaaaaaattg
    12241 cttcgttaga cgatcctatt ggaaaagtag atgagatgtg gacgaacacc gacggattga
    12301 gaatcggcaa aaagcgcgtg ccactaggcg tgatcggcat catttacgaa tctcgtccaa
    12361 atgtcacgac agatgcagct agtctagcat tcaaatcagg gaatgccgtt atccttcgcg
    12421 gtggcaaaga agcattcttc tcgaatcaat tattagtcca attattgcag caagtactgg
    12481 tcagtgctgg cgaatctcct tatgccatcc agtttgtcga tgatacttct catgaaactg
    12541 caggacagtt gatgcgattg acagaatact tagatgtgct gattcctcga ggcggagcaa
    12601 aattaatcca acgagtcaaa gaacaggcaa cggtacctat cattgaaaca ggtacaggaa
    12661 ataatcacgt atatatcgat aaagaagcac agttatctat ggcagtgaac atcatcgtca
    12721 atgcaaaagc ttcccgtcca tctgtctgta acgcggcaga aacattgctg atccattctg
    12781 agatcgcacc ttttttcttg ccagctattg aaaaagaact ggtagaacac ggggtctctt
    12841 tacgtgcgga tgctcgtgcg ttagaatatc tagagacggc tgctttagca gaagacacag
    12901 attgggatac tgaatatcta gattatatcc ttgctgtcaa agtggtcgat tctttggatg
    12961 aagcaattgc acatatcaat cggcataata ctaaacattc agaaacgatc gtgacagata
    13021 gttatgcggc tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg
    13081 cctccactcg ttttacagac ggatcggtat ttggttttgg tgcagagatt ggtatctcta
    13141 ctcaaaagtt gcatgcccgt ggtccaatgg ggttaaatga attaacttcc acaaaatata
    13201 tcatttatgg cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt attcccgcct
    13261 tttgcctttt ttctgtaaag gttctatcca actagaaagg tgtcaatatg tttgaaattt
    13321 taatgatttt ttttatttac cttatttctc tgaatatcgc tgcttttctg ggagtcggta
    13381 ttttatccct tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt
    13441 cgcagtattt tgacaaaatc gggcaaaaag ggctggttac acgcttgcac attagttaca
    13501 tggttgcatt atgcttatta gcgacaatca attactattc ttttttcgat cattcaatcg
    13561 cttatacgat tacgctactg attgctggga tttttcactt atcttataaa tatcagctga
    13621 ataaaaacca cttgaatcgg actttccgct aaaaaatatc cgtacgataa gtagagtgcc
    13681 ttgaagatcg gcaaaaaact gccggacttt ttagaggctg tgacaaagat tttgtcacag
    13741 cctctttttc catataaacg gcgttcgcct accagctgcc tggtaaatct gtttcctctt
    13801 cgttaagaag cgaaaatccg tcaaatgttt cgccatcata ataacgaagc agctcatcca
    13861 tggacaagtt catcgttttt tctttttctt cttccgctga ttcagccagt agctgttccc
    13921 gatacacctg atacatacca gctatttttt gctgcatcgc ttttctagtt tcaccttccg
    13981 cattgattat tggcaattct ttgatatagc cacgataaat caaccgatca tctgaaaatt
    14041 caatgggtaa ttcaacgagt gtcaacccga cttcctgaaa atattcttcc agcatctatt
    14101 atccccccat tctttttctt aaatgaattt tagcttacct actgaaaatt ttccagtttc
    14161 attattttga gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat
    14221 aaatggtgtt cttctgccaa ctcctcaatc ataagtcaca atattcaaca aacatgagaa
    14281 actgtttttt gaaattgttc cttgtgattc agagttaaac gacagtctca caacctcttg
    14341 gtaaacggcg ttcaatcagc tgaccctcgg tattagctca aaaaacggta aaatatgaga
    14401 agctattttt ccgttttgtt tctctaatac ttaggataaa acgctgattt tacaacctct
    14461 gttaaacggt gttcaatcag ctgtccttcg gtattagctc aaaaaacggc aaaatatgag
    14521 gaactatttt tccgtttgtt tctctaatac tcaggacaaa acgctgattt tacaacctcc
    14581 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
    14641 gagaagctat ttttgtttgg ctgttcttat gacttgatgc aggacagctt tttcacaacc
    14701 tctgatttct tattcttctt catttgtagc aaaataatta cggtgataaa tgatacaaca
    14761 tacactgata agaacaatgg cacctgcaaa ataaaacgca tagctttcac caatatgttc
    14821 accaatcaca cctgcaataa atggtgctaa agctccaccg aaaaagcgga caaaactata
    14881 gctggacgaa gcaacgtttc gttgcagacc tggtatttcc atagcaatcg tcgtcaacag
    14941 tgtattgata attccttgga aaaatcctgc taacacaatg ctgatcggta caaggactgg
    15001 catagaagtt ccagctccta ataacagcaa acaaataatg aataagacaa atgctgctaa
    15061 aattgtccga taagtcgtaa acttccgctc aagagccggt gcaacgaaaa tcgaagcaat
    15121 agccagaagc acaccccagc caaaaaatac aaaaccgact tctaattcag aatagccaat
    15181 caataaaaat ggcgcatacg caagtaaagt aaagaatccg aaattatata acaatgcgat
    15241 tagtccaact gaacgaagtt tatgattgct taacgctttg acaccttccc aaaggcctac
    15301 ttttctcttt ggtttctcag tttcaggtaa aagtaccgct agggctataa aaccgcaaaa
    15361 catcaaaaat gctaccccaa aaaatggata acgccatgaa ccactgccca agaccccacc
    15421 gactagaggg ccaacagcca ttccgcagcc aagtgctgct tcatacatca tgattgcttt
    15481 ttctgtgtta ccaactaaaa tactgacgat cgttgctaaa gcagtagaga caaataaagc
    15541 attcccgaca ccccagccgg cacgtaaccc aatcagcatt ccgatattcc cagataatcc
    15601 gccaagacca gcgaatacaa cgatgataaa caatcctgtc atcaatgttt ttttcaaacc
    15661 gatccgacta gataagaaac ctgtaaatag catcaccaca ccagtgacta acatgtaact
    15721 agtaaataac agggtcgttt cagctggcgt agcgtttaac tgtgcagcaa tcgtttttaa
    15781 gattgggtct actagaccca ctcccataaa agcgatcata caggtagctg ctacaactag
    15841 cgctgaactt ttcccctttg tttcttcatt tgtttgttct tgtatatcta tttgattctc
    15901 caatatttcc atccctttct ctctattagc atcatacatg atttatttta catgtcaata
    15961 tgcacatgta aaacgtggaa actttactgt gatataataa agaaaataag acgagaggta
    16021 ggatttttat gaatacttta gcctatatat tattatgcgt gcttttatcc aaaccacgtt
    16081 ctgggtatga attgaagcag ctgatcacaa ttttttggga agcccatcac agtcagatct
    16141 atacaacgct tgccaagtta gaccagcagg gttatgtgac cgttttggac aaagacgagc
    16201 actctcaaaa aaagatctac cacttgactg atgaaggacg tgctcttgta gaggaatgga
    16261 tcaaagagga aactcccgct cctacgcaaa aagatgaatt tctagcaaaa atctatgctt
    16321 tttcgacgct cgataaaaca acagctactg gactgctatt cactagagaa caacagctga
    16381 acaaaatcat gcaaaaaaat caaaaaaaat tgaatgacct ctcgctagac aagaaacaag
    16441 aatttggacg ttatgttgtg atccagcgca gaattatgct ttgtcagcag gaactccaat
    16501 ggtgtcagca agtaagagaa caaatgcagc agttttttga gtaagggata aacagaacct
    16561 ttctagaaat atctattgaa gagcaaatta tcttcaataa aagagcaata cagttggaaa
    16621 agaaaaagcg atccgaacga caggccaaaa cctatcgttc ggatcactat ttttctagaa
    16681 ctgcattcaa gagactatga caaaaagcat gccaaggcct tttttcaaat aacagtgttc
    16741 catcattcat tactttgaat tgcttttagc acacttcttg aaagcgggcc aacgatgatc
    16801 aactgcaacg gtaaagcggc aatcacattc attttccaag cattcatata agcagagaat
    16861 aattgatcag gtattccttg ttccatgatc acaccgaata aagacataca tgtcaccatt
    16921 cctaaaacca tcaagcatga aatagttaag atcatttgga ccttgctttc tggatttact
    16981 ggtagtttaa aggcaatttt tttcgccagc acaccgatga tgaagacatc caaaatgaaa
    17041 gccaccacga atccaggcac tagaccaatc aacaaacttg ataaatgaag atcttgatgc
    17101 agccataaat tatacgcgct catccccaag accatcaaaa aacacatcat tgttgtaaaa
    17161 attattcctt cttttttatt tgtaggcaat ctgtaccact cctttaaatt tgtcacgaaa
    17221 atcatcgtag cacatttaaa aatttctcgt aagtactttt tttattttct gcccttttat
    17281 ttcataaact tcatcagccg tacgctcgac aaagtaagaa tcatgagaag taaacaaaat
    17341 cgttcccgga tatatttgca ttagcttctc taaagcttcg atcgttgcca tatcaataaa
    17401 attagtcggt tcatctaaca gcaacacatt acttggctta gtaaaaagca aagcaatcgt
    17461 cagacgggtc gcttctcccc cacttaatgt acataacggt ttcgtcaaag tttcctgagc
    17521 aaaccctaag tgattcagca aactacggac aagcgattct gaaaactctg tttgtctcat
    17581 gacaaaacga atgatggatt cttcagatgt catttgataa cccttttgct gatatacttg
    17641 aaagcttact ttcggagaga gaaggattcc ttctccttgt ttgcggatat gttctaataa
    17701 ggttgtctta cctgaaccat tttcaccgac aatcgcgatt tttttaccta aagaaaactg
    17761 aaaatcacca tttaccagta atgttctccc acttctttcc aattggacat tttgtcccat
    17821 gatgggatag cggctgtgga gttcaagaga ttttggtact ggaaaacgga tttgtttcat
    17881 ctgctgcggc ttggttgttt ctcctatttt ttggagtctt ctctctaatg ttttcgcatt
    17941 cttttggatg gctttttgta cactgtcttt ttgtttagag gaagacaacc gatccagtct
    18001 gatttgttgc tttttctttt tactggacac tttttccaac ttttccgctt gttttttctt
    18061 ttcttggatc gatttttcca atcgtttttt ctctttcatg atcgactctg cttctcgtag
    18121 ctgagtcttt ttctccaatt ccttttgagt gagataggca ctataattcc ccgaaaactc
    18181 tcggatttct ccgtctttta cttcccagat tttcgatgct agctcatcta gaaaatagcg
    18241 atcatgacta acaaaaacca gcgtaccata ataataacga agctcttcaa tcagtcgatc
    18301 aatacttttt ctatctagat gggttgttgg ctcgtctaga agcatccctt gttcataaga
    18361 agaaagaaga cgtatcaact cgatctttcg ttcttcccct ccgctccatt tccgctctga
    18421 agtttcttcc agtcttaact ggaaatgact ggctaattcc aaatttaggt cctttgcttc
    18481 agcgagatag gttaattgag gcaggtagtt gaaggtgatc tctgtttgga ttttcccatg
    18541 atcgggaaaa agttcaccgg ctattagttt gagcaaagtc gatttaccga caccattttc
    18601 tcctatgatc cctatacgat cttgctgata gacacgaaga gaatcaatag atagttctag
    18661 ctggttgcca aagctgactt gtagatttgt tatatttact gctaaatttt ccatatgttt
    18721 tacactccta tttcatgtaa aacaacaatt atcgatacta aaaaagagca gaaaaatctg
    18781 tttgcaaaaa aactgcatca catcttttcc tactctgaac gaaataacta tgactaaatc
    18841 accaaatctg tcatagcttt cacctattct actgtcgttc gaaaaaatgg acatactcca
    18901 tccatgaaat tgttgtttat acatgtcttt tgaatttatt tagttgttat tcaaaagttc
    18961 gaaacgtaat ttcatcgatg cagtcatttt tccgacctcc ttttttatta ggttaccaca
    19021 cacgattcaa aaaaagcaat agctactgaa tagtaactga aaacctttta ttcgaacata
    19081 gtgatctttt ctggtgctaa acgaacagac tcgtaacctt cttctaccgc ttttcctatt
    19141 gataaaataa taactggaac ataacggtct ttatccaaac caaatgcctc tgccaactga
    19201 tctgcttcga atccaccaat tggatttgtt tcatagccat gatcacgcgc aactaacatg
    19261 aattgcatag ccgctaagct agaatcgact ttgactacat cgttcatttc ttctcttgaa
    19321 aaactcttgt aataaggaat gatcgcagct aattgttgat ctctgacttc ttttggcatc
    19381 ttccctgatt cgtaagcttg gttgtagatt tcttcgccat attcgtaaca ttgcatatcg
    19441 ccaaaaatca tgaccattgc agaagaagta tcattttgtc tcgtattgaa acggatcaaa
    19501 ggacgcaaga tttcttttgc ttcctcgctt tcagcgacca caaagcgcca tggtttcata
    19561 ttgacagaag aaggtgcagt cgtcgcttct ttgatcattt ccagcatttc ttcgtgtggg
    19621 attttgtatg tttcatcata tacgcgaacg gatcttctac cagtcataat tgttgaaaaa
    19681 tcattttttt gttccattat tattaacctc tttcgtttct gttgttatat tgcaaagttg
    19741 ggtattcaat ttatctaaaa gtgttagaaa ttgtttcatt tctacatcgc tgaaagccgg
    19801 ggatagcacg gaacctgtat ctttatggtc catttcacaa tgcttgattt tcttctgggc
    19861 tttttctgtt agcgctacat acacttcccg gttgttctga ggatttctct gacgcgaaac
    19921 atagccattt gcttccaata ccttcagatg acgagtaact gctccttgat caatctgcaa
    19981 cttttcttgc aaactgcttt gtagcaatgg attttcttct ttcagtctca tcatgatctc
    20041 gtatctagta agacttatac cgatattttc ttcaaagatc tggacgattt tttgatcggc
    20101 taatttgatt tgatataaaa ggtgactaat ctcttctaaa gacataattt cctcctttct
    20161 aataattgac tcgtcaacga ttgacttgtc aataatagaa taaaaaaaga aaaaagtcaa
    20221 agaatcagct gtgtaataaa aaaaacgctc tcaatagaaa agtatagaga aaagaaattt
    20281 tttgtagaat gaacggaaaa aatgataaag aaagcacaac gctaaaacca agattattga
    20341 ttcttatcaa actatagaag atagctcttc tgcagtagat aattttacac cgccaaaaat
    20401 ttccgaaatc gccttttgca agaatcccgt cgaatattag aaattggatc agtatgctta
    20461 aacagtaata aaaaagttat gacaagatgt tgtcacaacc ttttttgagt aaacggtgtt
    20521 caaaaacttg catctgcggt aataagttca accaaacaaa aaacatgaaa atctgttttt
    20581 gtttggctgt tcttactact tgatgcaggg cagctttttc actacctctt tttaataacc
    20641 ataaaatcct attccttcat atctccagcc catcggtgtt agccaatctc tttccttagc
    20701 actcaaggta tagtgatggg taccagtttt ttcatttgga ttaaatagtc gataaattgg
    20761 cttggaacca ccagaataaa aacttagacc ttcataacgc caaccgattt ttgttaaaga
    20821 gttgatctca gattgtgcca ttgtgtaatg atggtctcca ctgtaaggat tgtataaacg
    20881 gtagacaggt tgtccgctgt ttggtgcttg ccaagcaatc ccttcatatt cccagcctac
    20941 agttggcaga taattttttt ctccggcatt caatgtatat aaatgttctc ctgtatgacg
    21001 gttatacaga cgatacatcg gagaagttga agttggtgct gtatcatact gtgtcagatt
    21061 ataggtttcg attacattgt tcaatgtaga accataatta ggcgcagtgg cataacgacc
    21121 tgttagccaa gccgtagcat ctttgtaaga gttcgtattg cttttccatg caccggaata
    21181 gtagtaaacg cctggctgaa aagaagtcgt cttcaatacc gttacgttat cttgcaatga
    21241 ttctttgtac gaaggatatt ttctaaaagc ggcattcacc gtaatccatt gcccattcac
    21301 atattccgaa gtaggcatcg taacagattg gccattatag cttcctttaa tcccaaatag
    21361 attgtaatta ggggcagaag ccaaagtact gttcccccag ccactttcaa cgatggcttg
    21421 agcaatcatg actgatgcat ataagtcatt ggcacttgcc aaactttgtg cagttgaggc
    21481 aatactatca atgaacgctt gttgagaatt agcagatgag cgagcataaa aagcatagcc
    21541 agtatagttt tcgaattcag acaaatctgc ttgatgggta tgtgtcaatg tttgcccatc
    21601 tgtcaattgg ttcgtgattt cttctgcatt ctctggattg ttctgagctt ctgctgatgt
    21661 actgtcttcg gtttgtgttt gttctgttgt ttcagttgtt ggttcttcgg ttgaaattgt
    21721 attttctgag acagtagctg actgcaaagt actttctgta tcgttttcaa ttcctgtttc
    21781 tgaagaactc gatgtctgag ttgcgacagt ttctgtcgat tcttcagcta gcactgttgt
    21841 gttggcaact gctagatttc ctaaaagaat aacgctaaat aataaattct ttgctttcat
    21901 cttttttctc ctttgaatta tgaaactttt cctaaataac agtgtaacta aaaaagtaaa
    21961 agcaagtcaa tgtccatatt taaataacat ttgtttcttt ttaataaatt tgctttttct
    22021 gcataaaaag acgtaaatga agaatattct tctccattca cgccttaaat tgatggtcgt
    22081 ttgaataact atttaacaag taattctctc tggatattat ccgttatgga ttatacttcc
    22141 tgtgtcgtcg gacggataat catgttgctg acagacatcc ggtctggcgt ttctatcgca
    22201 aatgctacag cagaagctat atcttcagat gttaatcccc attctttttg cgcttcatga
    22261 agttcaagag cagccttttt gtcgctgatg gtttgatata attcagtttg aacagctcct
    22321 ggagagatga tcgtagattt gatgttattt tgccgttgct cttgacgtaa tccttccatg
    22381 attgcacgga cagcaaattt tgttccgcaa taaacggcgg aatcaggata aacaacatgt
    22441 cccgccacag aatcagttga aatgatatgc ccagattttt gtttttccat gattggaagt
    22501 acggcggaaa ttccatttaa cacacccatg atgttgatat caagcatatt ttgccattca
    22561 tcacggtgac cttcaactaa tggagcggtt ggcatgattc ctgcattatt gtacaataca
    22621 tcgattcgac cgaacttttc catcgctaaa tcgataactg cttggacttg tgcaaaatct
    22681 cttacatctg ctgtttgaat atatagctca gcttctggta aagattcttt tattgctttt
    22741 aggcgatctt ctcggcgtgc agctataact agtttagccc ctttttctgc taatagcttc
    22801 gttgttgctt caccgattcc gcttgaagcg cccatgatga caatcacttt ttctgataaa
    22861 tcattcattc tattctcctc ctgttttgaa aaatctcttg cttatactca ctatctctta
    22921 tttcatctta ctgttcaaat aatccgttcg acaggtttga cgattttggc agggattcct
    22981 gcaacgatgg tattgtctgg gacatctttc gtaacagttg cattcgctgc aacaatggca
    23041 ttctcgccaa tcgttacgcc tggcagaata gttgctcctg ctccgatcca agcatttttc
    23101 ttgatgtgga ttggctttac tgacaagcct cttcgttctt ttggatcggt tagatggttc
    23161 accgtgatga ggttgacacg aggaccaatc aatactgaat cttctaaaca gatgccacct
    23221 aagtcaacga acatcacttg acgattgata aagacatttt tccctatact aatatgtttg
    23281 ccgaaatctg tataaaaagg cagcagaaca gtcaccgtat catctacttt gtcactaatg
    23341 atattttcta gccgtttgcg gatatctgct ggttcatggt acccagtatt cagcgctgct
    23401 aaaggctctt cgttttccgc ttggatctga tggatcactt caaagagcgg atccgttact
    23461 cgaatctctt tattgacgat ttgtttttcg atgaggcttg tcattttgct ccctcctttt
    23521 atccttactt tataacggac ggcttatgat gtctaatact tataaaacat acttaactat
    23581 tccttttaac tatacttagt ggtatgcttt tttaaaaaca ggagggaaaa aatggatatt
    23641 cgtatactga cgtatttttt agcagtagct agagaaaaaa atatcagccg agcagcaaaa
    23701 tctctgcata tcacgcaacc tactctctca aaacaactga aagatttaga agaagaactt
    23761 ggtgtgacac ttttcactcg aggctcccga gagatacagt tgacggattc tggtcgatat
    23821 ctttattcaa aaggaaaaga aatcttgtcg ttggtagaaa aaacggcgaa caatttaaca
    23881 aatgacgcaa tcatctctgg agaaatctac attggcggag gcgaaactaa agcaatctct
    23941 ttcatttctg atagccttca ttcgttgatc aaggcgcatc ctgatatcca gtttcattta
    24001 tatagcggga atgcagatga tatcatagac aaactggata gcggattact tgattttgga
    24061 ctcgtaatcg aaccgacaaa caaacaaaaa taccagtcat ttcgactgcc agcccaagat
    24121 cgttggggac ttttgataca tcggaatcat cctttagcaa agcaaacagt catccatccg
    24181 caggaactga aaactgttcc tttgatcatt tcccggcaaa cgtcagtgga cagtcaacta
    24241 gctgaatggc taggtttcag catagatcag ttatcgatcg tcggaacata taacttattg
    24301 tacaatgctt ctttgatggt cgataacggg acttttggtg cattagctct agatgggatc
    24361 atcaatactt atgggacgga aaaagtattc atcccactcg atccgccatt acaagcttca
    24421 ctgaatctca tttggaaaaa agaccagcct ctttctcctg ctgctcgtaa atttttagat
    24481 gtcctaacgg agaaatacag ttaagtagag tttttttcct tcgctttcag ctttttaaca
    24541 acgaataagt taagcaaaag atccaatgat taaataacat aagtttctta atttgttcct
    24601 tccttttatt tctgataatt ccagtaaatt actataatta taaaaaaaga attgacaaaa
    24661 cataactgtc tatataaaat tgattacgtt aacaataatt cgtttcatgt gactagatca
    24721 tactaggcat gaaaaatctc tgaggaacat ggtttcccta ggaggttttt cgtgtctatt
    24781 tttgtttggc cgcaatcaac tggtctagca aacgtgaaaa ccaaatcttc aaaaagggtg
    24841 cgcgcatgaa aatcaaaaag atctttaatc aaaatgcagt attggtagat gacggtggac
    24901 aggaaaaagt agctattgga aaagggattg gatttgacag aaaaagaaat gatttgattt
    24961 ttgatcgtga tgtcgaacgg atatttgtca tggaaccaga aggtcaaatc aagttgcaaa
    25021 atttacttaa tcagattgat gaacgctttt tatttgcagc agaaacgatc attgatcatg
    25081 cggaaactgt tttgatggaa aagttgaatg aacatgtatt gatcgctttg accgaccaca
    25141 ttgctttttc tgcggagaac attaataacg gtatcgtcat ccgcaacaaa cttttaagag
    25201 aaatcgaagt gctttacagt gatgaattca gtattgctca atgggcagta gagtatctga
    25261 caaaagagct tgggattcca tatacttatg acgaagcagg atatatcgca atccatattc
    25321 acagcggacg aagcggtcgg aacaataacc atcgcagtat ccgtgaagtg acgattgtat
    25381 ctgacatcat ccatttagtg gaatcagaat tggaaacaga tatccattca gaaccatttt
    25441 cactgaatta ttcaagactg gtgaatcatt tacgactgct tctacaacga acccatgcgc
    25501 aacaatatgc tgttttagac acggaaatcg tagaaatggt caaacgaaaa tacccagaaa
    25561 gctataagat ttctaaaaaa atccgtgtgt tgctgaccaa agaataccag ctcgcgatca
    25621 cgaaagaaga actgggttac ttggccatcc atatcgagcg tttaagaagc gcaatcgtac
    25681 aaacaaatgt taacaatcat gaggaggaaa aaaattgaaa gcatatctac aacgaatggg
    25741 acgttccctt caacttccag ttgcggtctt acctgctgct gctttactag tagggatcgg
    25801 aaactggtgg gcatcttata gtaacgacat tgtcgctcat tttttacaag ctggcggtaa
    25861 cgcagtttta ggtcaattac ctttgctgtt tgccgttggg ttagcactag ggatgtcagt
    25921 ggacaaatca ggtgctgctg ccttggcagg tttggtcgca tttgaagttc cagtaaatgt
    25981 tttaaaaact gattcagtag ctactttatt gaatatcaaa ccagaatcag ttgatccatc
    26041 ttttgctcag atcagtaacg tatttattgg tattattgcc ggattgattg cggcagctct
    26101 ttataatcgt ttccacgaag caaaattacc gatggcttta tctttcttta gcggcaagcg
    26161 tctagtgcca atcttggctg cctttgtcat gttgctgatc agtgcagtgc tactgctcgt
    26221 atggcctccg gtttactctg ctttagtttc ttttgctaaa ttcattttga gtttaggctg
    26281 gggtggtgca ggtctttatg gcttcttcaa ccgtttactg atccctacag gattgcatca
    26341 agcaatgaac tccgtgttct ggtttgacgt agcaggaatc aatgatattg gtaaattttt
    26401 ggcttctgaa ggaacaaaag gcgtaacagg aatgtatcaa gctggatttt tcccaatcat
    26461 gatgttcggg cttccagcag gagcatatgc tatttacaga aatgcacgtc cagaaaacaa
    26521 agcaaaaaca gcttccttga tgttagcagc agcttttgct tcattcttta ctggtgtaac
    26581 agaaccactt gaattctcat ttatgttcgt tgcttggcca ttatatgtct tgcatgcaat
    26641 tttcactggg atttctttag cagtgagtgc attcttccat tggacagcag gatttgcctt
    26701 tagtgccgga tttgtcgatt acttcctatc tttgaaaaat ccgattgcga atcagccatt
    26761 gatgttgatc gtccaaggat tagtaacagc agtgatttac tactttggct ttaactttgc
    26821 tatcaaaaaa ttccatctga tgactcctgg ccgcgaggaa gcagatcttt cagatgacga
    26881 tactgcaacg actaattcag acaataaata tgcggcacaa gcaagcaaaa tctacgctgc
    26941 attaggcgga gccgacaatg taacttcgat cgataactgt actacacgcc ttcgtttgca
    27001 agtcaaagat acaggcctga tcgatcaaaa taaaatcaaa gccactggcg tacctggtat
    27061 gaagatcatc gacggaaaaa atgcgcaagt catcgtaggt acagaagtac aattcgttgc
    27121 agatgagatg gcaaaactac atggtggtgc tgcaacacga cctgcacaaa caaacaccgt
    27181 ggttaaaaca gaaacatttg ccggtggaac gatcgaacaa gatatttacg ctatcgcaaa
    27241 tgggaaactg attccaatca ctgaagtttc agatgatgta ttcgcggaaa aaatgatggg
    27301 tgacggctat gctgtattac cagaaaatgg agaaatcttc tctccaatcg ctggtacgat
    27361 cacaaacatt ttcccaacga aacatgctgt cggaatccaa acagatgctg gtatcgaagt
    27421 cttgcttcat atgggcatca acactgttga cttaaaaggc gaaccattca ctttatatgt
    27481 aaaagaaggt caaaaagtcg caagaggtca attgattgct ttagttgatc ttgccgctat
    27541 tcaatctgca ggcaaaaaca cggatatggt cgttgtattc acaaatggcg acaaagtcca
    27601 aagcttagaa atcgaacccg cacgtgatgt aaaagcaaat gataaaatcg gctctgtttc
    27661 caacaaagcc tgagccatcg tcttccccag acttttttat cgataaacaa acaggagctg
    27721 agacaaaaat cttgtctcag ctcctttttc cgaataaaaa cggcgttctc ctgccaactc
    27781 cgcggtaata agtccagcca aacgtaacat atgaagagct attttttatt tgactcttct
    27841 tattacttga tgcagaacag cttttttaca acctctgata aacggtgttc aaaaaccagt
    27901 ttatcggtat gaagtcaaat ttatgcaaaa atgtggaaag ccattttcac aaaatttttc
    27961 ttcatactca aaactaatcg gctttttcac aacctcttta tacattattc ctgatccaat
    28021 atttctttta atgctgcttc aagagaagtt aatggttttc ccaatacttt ttctaaatca
    28081 tcagaggaaa tagctaactg atccttttta atatcatgtt ggatcgctaa aaacatgtca
    28141 gctgcttctt gagggaatcc ggcttctttt agattttcga caaacgctgc atcacttgct
    28201 tcaataattt ctaacttttt acctgtcgca cgctctaatg ccttggctaa ttcttcatag
    28261 tcagttagat tgccggataa ttctaagatt tctggagagt cactttctac tagtactttt
    28321 gctgcagctt ctgcatattc acgtttcaat gcccaacctg tttttccatt tcctgcagca
    28381 taaacgaatt gaccagtttt taaagcctgc ccaatgatag gtaattcatt ctctacatac
    28441 caattattcc gtaaaaatgt atgggaaata cctgaatcct taatcatttt ttcagtgaat
    28501 tggtgatctg gcgctaatac actgatcgag tgatcggcat ctgcaaaact tgtataagcg
    28561 atataagata ccccagctgc ttgtgctgcc ttcactacat tcttatgttc ggcttgacga
    28621 tttcctggaa cgccagagac aaaaagcaat cgatctattc ctttcaacgc ttctttcatt
    28681 gagtctaaat catcataatc accaatacgt atattaaatc cagcttcttt cagtgcttct
    28741 cctttttctt cactacgtac tagtacatag atttcatttt gtggaaccat cttttttaag
    28801 aattccagcg cataaccacc aaaacctcca gttgctcctg ttactaaata atgcatattt
    28861 gccactccct attcttttta tttgttaaga tttacagctg tacatttata aattacacct
    28921 ttatcataaa gagtgtggta agataagtca agcaaaaaaa atgaaaggat gacaactgat
    28981 gaagtattct ctgcaattta gtgatgcaat ccatgtactt gcctatattg aaatgtttca
    29041 agatacccga tcactttcta gtgagatgat tgccagtagt atcgaaacaa atgcagcaaa
    29101 tgtacgtaaa atcatgagtc agctcaaaaa atccggtttg atctttacaa aaaccggaaa
    29161 accgcagcct tcccttgcca aaagtccaga tcagatttct ttactagatg tctatcgcag
    29221 catcgaaggt aatacacagc ttatccaagt cgatccaaaa acaaatccaa attgcgtcgt
    29281 tggtgcgaat atccaagaag ctttagaaga aagctacgct cggctgcaga aaaaggcaga
    29341 agaagaaatg gctaagatca cacttgatag tctgatacat aaaatagccg ttttggaaaa
    29401 agaaaaaaga ccagaaaaca caggcctcgt agagagattt ttataattct ctctactgtg
    29461 cctgcatttt tctgatcgga atcgtctaca taaattcttt tattctccgg gggacaaaag
    29521 ccttgtcatg cccctttttc ctctctaatg ctcaggtcaa agcgctgaag tcatgacgtc
    29581 ttgataaacg gtgttcaagt agctgaccct cggcattagt tctgaaaaat gcaaaatatg
    29641 gaaagctatt tcccattttt cctctctaat gctcaggtca aagcgctact ttcacgacct
    29701 ctatattatc atggtttatt gactaccggt atccagattt ctcccatata tttcccgttg
    29761 gtaactccca tttcgactac agcatttgga cccccaacat aggcgtaatc ttctacttct
    29821 cttaaaacat gcccaaaggc cgtattcgtc aaatcatttc ttaattcttc aggcgattct
    29881 gctaaagcga ttacaacaag atacgttcct tttgggaatg ggatgattcg ggtagtgttt
    29941 ggaacatcct gactcgtttg aacaccgaca tagtacatca ttttcccgtt atagggttcg
    30001 ttgactgtaa agaaataatg attttcagca gcttgtttca atgcatctat tgttccatta
    30061 ccgataactt tttgctcaaa tgcttgtttc tctgcagcca aaccgacaaa atccgtattg
    30121 ctactcttca attccacacc aatacccgtc accgtaaaat ctggtttttc ttcgatccta
    30181 tactttgcca tttctcccac cctttctatt atattaagtt catcttacat aaaattaaag
    30241 ggaaatgaca cgattacgct cttttttcta gggatatttt tattttttaa ctattttttt
    30301 gctttcactg caatccaaat ctcactgtaa acattactga aatcctctcc ccattttgta
    30361 aaagagatct cgggagcttc gactaactca taatctgaag aaggaagcca ttctgcatag
    30421 attttccccc aagtatcttg taaagtttgg ggaaatggcc ctttattagg aaaaatggcc
    30481 caagtgtgtt cttctatagc aagcatttct aaatcagcgt acggattttc ttttgtcgta
    30541 gcaaagccaa tcatgtgcat caaactgccc ttttcttcca agcgggctcc atcaaatcca
    30601 taagaaacgt tgattacttg atttggatat aaatctccta acttatgcat ctcttccctt
    30661 tgagtctccg tgatcgtctg cgctaacttt tgtatttctt gattgactcc ttcaaatttg
    30721 acaggaactt ttttcgccac acctgccaca taaaactgtt ctttcttttc gatacgaaat
    30781 tccatcgatt ttcctcctct gacatcaata aaaaatgtca agcgtggaaa agatttttgt
    30841 atgccgtatc tgctgatttc tgaaggcaga tatcctgacc aatcacgaaa agctcttgaa
    30901 aaaccttcga ctgtttcata tccatatcta aacgccgttt ctgtcactct tgatccatct
    30961 aggagatcgc gattagccaa agccaatcta cggtttttga tatagtccat tagcgacatc
    31021 cctgcaataa acgtaaagat gcgttttaat tgatattcag agattcctgt attttcggct
    31081 attattttat aggaaagtcc ttctgtcaaa tgttcttcga tgtaatcgat caattggttc
    31141 agatctttaa gcatatttat cttcctcctt gcttgccttt ttatcatatt atcaaagaaa
    31201 gtcaattact tgatgaatca ggaaccaatt tagtcagatg tagaaaatgt tgtatggatc
    31261 taaagaaaat tctcaagcag cgtttcgtaa cggataattg gtattatctg cctatctatg
    31321 ttttaccttt agaaaaataa attttgggga agacacattc taagtaaaca aagcggtaaa
    31381 aatatctttt attcccttag ttaatccgct tactacctac tgtacaaagc aaattgttgt
    31441 tcaaactgct gatcagaacg ctgcttggat ttttctttgc gatacatcat acctctttta
    31501 tcaattggaa caattattta tttctaacaa aatcacttca tttttgtcag ataaataaaa
//
LOCUS       DAJSBN010000029        31245 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJSBN010000029 DAJSBN010000000
VERSION     DAJSBN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.029"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            202..1077
                     /locus_tag="ONT86_001546"
     CDS             202..1077
                     /locus_tag="ONT86_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352347.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="ONT86_001547"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="ONT86_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="ONT86_001548"
     CDS             complement(1642..2973)
                     /locus_tag="ONT86_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2352348.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="ONT86_001549"
     CDS             complement(3122..3835)
                     /locus_tag="ONT86_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2352349.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="ONT86_001550"
     CDS             4010..5434
                     /locus_tag="ONT86_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2352350.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="ONT86_001551"
     CDS             5733..6440
                     /locus_tag="ONT86_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR2352351.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="ONT86_001552"
     CDS             6427..6750
                     /locus_tag="ONT86_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR2352352.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="ONT86_001553"
     CDS             complement(6839..7876)
                     /locus_tag="ONT86_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR2352353.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="ONT86_001554"
     CDS             complement(8197..9180)
                     /locus_tag="ONT86_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCR2352354.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="ONT86_001555"
     CDS             complement(9595..10365)
                     /locus_tag="ONT86_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2352355.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="ONT86_001556"
     CDS             complement(10358..11293)
                     /locus_tag="ONT86_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2352356.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="ONT86_001557"
     CDS             complement(11609..13258)
                     /locus_tag="ONT86_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR2352357.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="ONT86_001558"
     CDS             complement(13375..15978)
                     /locus_tag="ONT86_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR2352358.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="ONT86_001559"
     CDS             complement(15990..17039)
                     /locus_tag="ONT86_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR2352359.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="ONT86_001560"
     CDS             complement(17065..17379)
                     /locus_tag="ONT86_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCR2352360.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="ONT86_001561"
     CDS             complement(17395..17841)
                     /locus_tag="ONT86_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2352361.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="ONT86_001562"
     CDS             complement(17842..19764)
                     /locus_tag="ONT86_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCR2352362.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="ONT86_001563"
     CDS             complement(19976..20872)
                     /locus_tag="ONT86_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2352363.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="ONT86_001564"
     CDS             complement(20865..21545)
                     /locus_tag="ONT86_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2352364.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="ONT86_001565"
     CDS             complement(21546..22328)
                     /locus_tag="ONT86_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2352365.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="ONT86_001566"
     CDS             complement(22321..23238)
                     /locus_tag="ONT86_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2352366.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="ONT86_001567"
     CDS             complement(23402..23725)
                     /locus_tag="ONT86_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352367.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="ONT86_001568"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="ONT86_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCR2352368.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="ONT86_001569"
     CDS             27853..28167
                     /locus_tag="ONT86_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2352369.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="ONT86_001570"
     CDS             28180..28554
                     /locus_tag="ONT86_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2352370.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="ONT86_001571"
     CDS             28564..28899
                     /locus_tag="ONT86_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCR2352371.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="ONT86_001572"
     CDS             28982..30322
                     /locus_tag="ONT86_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR2352372.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="ONT86_001573"
     CDS             30400..31074
                     /locus_tag="ONT86_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR2352373.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31067..31231
                     /locus_tag="ONT86_001574"
     CDS             31067..31231
                     /locus_tag="ONT86_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352374.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
BASE COUNT         9736 a         6069 c         5515 g         9925 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaat
//
LOCUS       DAJSBN010000030        29981 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJSBN010000030 DAJSBN010000000
VERSION     DAJSBN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.030"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            137..475
                     /locus_tag="ONT86_001575"
     CDS             137..475
                     /locus_tag="ONT86_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352375.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(680..1255)
                     /locus_tag="ONT86_001576"
     CDS             complement(680..1255)
                     /locus_tag="ONT86_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCR2352376.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1987..2568)
                     /locus_tag="ONT86_001577"
     CDS             complement(1987..2568)
                     /locus_tag="ONT86_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2352377.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2751..3377)
                     /locus_tag="ONT86_001578"
     CDS             complement(2751..3377)
                     /locus_tag="ONT86_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2352378.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3399..4730)
                     /locus_tag="ONT86_001579"
     CDS             complement(3399..4730)
                     /locus_tag="ONT86_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCR2352379.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4885..5286)
                     /locus_tag="ONT86_001580"
     CDS             complement(4885..5286)
                     /locus_tag="ONT86_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR2352380.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5372..5578)
                     /locus_tag="ONT86_001581"
     CDS             complement(5372..5578)
                     /locus_tag="ONT86_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352381.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5673..7175)
                     /locus_tag="ONT86_001582"
     CDS             complement(5673..7175)
                     /locus_tag="ONT86_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCR2352382.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7193..7648)
                     /gene="argR"
                     /locus_tag="ONT86_001583"
     CDS             complement(7193..7648)
                     /gene="argR"
                     /locus_tag="ONT86_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR2352383.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7746..8363)
                     /locus_tag="ONT86_001584"
     CDS             complement(7746..8363)
                     /locus_tag="ONT86_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR2352384.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8584..9957
                     /locus_tag="ONT86_001585"
     CDS             8584..9957
                     /locus_tag="ONT86_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2352385.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10032..11144
                     /locus_tag="ONT86_001586"
     CDS             10032..11144
                     /locus_tag="ONT86_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR2352386.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11216..12853)
                     /locus_tag="ONT86_001587"
     CDS             complement(11216..12853)
                     /locus_tag="ONT86_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR2352387.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13021..13629)
                     /locus_tag="ONT86_001588"
     CDS             complement(13021..13629)
                     /locus_tag="ONT86_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2352388.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14013..15164)
                     /gene="queG"
                     /locus_tag="ONT86_001589"
     CDS             complement(14013..15164)
                     /gene="queG"
                     /locus_tag="ONT86_001589"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR2352389.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15183..15935)
                     /locus_tag="ONT86_001590"
     CDS             complement(15183..15935)
                     /locus_tag="ONT86_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352390.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16076..17026)
                     /locus_tag="ONT86_001591"
     CDS             complement(16076..17026)
                     /locus_tag="ONT86_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR2352391.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17027..17866)
                     /locus_tag="ONT86_001592"
     CDS             complement(17027..17866)
                     /locus_tag="ONT86_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR2352392.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17863..18609)
                     /locus_tag="ONT86_001593"
     CDS             complement(17863..18609)
                     /locus_tag="ONT86_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR2352393.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18847..20217
                     /locus_tag="ONT86_001594"
     CDS             18847..20217
                     /locus_tag="ONT86_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2352394.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20272..21168)
                     /locus_tag="ONT86_001595"
     CDS             complement(20272..21168)
                     /locus_tag="ONT86_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2352395.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21282..21452)
                     /locus_tag="ONT86_001596"
     CDS             complement(21282..21452)
                     /locus_tag="ONT86_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352396.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(21449..22126)
                     /locus_tag="ONT86_001597"
     CDS             complement(21449..22126)
                     /locus_tag="ONT86_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR2352397.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22217..23122)
                     /locus_tag="ONT86_001598"
     CDS             complement(22217..23122)
                     /locus_tag="ONT86_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR2352398.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23297..24751
                     /locus_tag="ONT86_001599"
     CDS             23297..24751
                     /locus_tag="ONT86_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2352399.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            24767..25798
                     /locus_tag="ONT86_001600"
     CDS             24767..25798
                     /locus_tag="ONT86_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR2352400.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25805..26992
                     /locus_tag="ONT86_001601"
     CDS             25805..26992
                     /locus_tag="ONT86_001601"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR2352401.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27148..28530
                     /locus_tag="ONT86_001602"
     CDS             27148..28530
                     /locus_tag="ONT86_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR2352402.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            28502..28888
                     /locus_tag="ONT86_001603"
     CDS             28502..28888
                     /locus_tag="ONT86_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352403.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29088..29756)
                     /locus_tag="ONT86_001604"
     CDS             complement(29088..29756)
                     /locus_tag="ONT86_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR2352404.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8404 a         6140 c         5483 g         9954 t
ORIGIN      
        1 taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca aatactatag
       61 ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa tttcaaagac
      121 aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct tttgctctga
      181 caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa ctgttcggat
      241 caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta gacaagactt
      301 tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt tacatgatcc
      361 aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag aatatacaac
      421 tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt cataatgata
      481 aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa atagaagcta
      541 tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta tgagcatttc
      601 ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt gccgctcttt
      661 ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc tgctgagtaa
      721 tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct tcagtcaaga
      781 caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta gtcattatga
      841 ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata tacactattt
      901 tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcataa aagccactca
      961 ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg
     1021 tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca
     1081 tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg
     1141 gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc
     1201 gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa
     1261 tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc
     1321 tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta
     1381 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt
     1441 ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg
     1501 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     1561 ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa
     1621 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc
     1681 tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca acctctccac
     1741 taaacggtgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa aatatgagga
     1801 actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca caacctctaa
     1861 tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac aaaaatttga
     1921 gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
     1981 taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg
     2041 cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc
     2101 aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt cagaaagtgc
     2161 caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg
     2221 gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata
     2281 agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc
     2341 tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg
     2401 cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc
     2461 ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt
     2521 ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct
     2581 ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt
     2641 ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata
     2701 gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt
     2761 tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac
     2821 gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg
     2881 aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca
     2941 ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct
     3001 ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat
     3061 tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca
     3121 aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca
     3181 tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt
     3241 accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca
     3301 aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta
     3361 tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga
     3421 gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt
     3481 ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt
     3541 gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct
     3601 tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg
     3661 ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat
     3721 acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga
     3781 tccataatga ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg
     3841 tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct
     3901 ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt
     3961 cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta
     4021 aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg
     4081 aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca
     4141 cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac
     4201 attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat
     4261 tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg
     4321 attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc
     4381 actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg
     4441 tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg
     4501 cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg
     4561 aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt
     4621 gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc
     4681 ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg
     4741 cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca
     4801 aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag
     4861 tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct
     4921 agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc
     4981 ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc
     5041 taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt
     5101 gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc
     5161 tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt
     5221 tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt
     5281 tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg
     5341 acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta
     5401 ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa
     5461 aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga tacatatata
     5521 ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt
     5581 tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc
     5641 gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca
     5701 gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca
     5761 tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag
     5821 ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag
     5881 ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt
     5941 tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc
     6001 aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc
     6061 aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg
     6121 tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct
     6181 ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca
     6241 atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc
     6301 tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca
     6361 gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct
     6421 tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg
     6481 atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct
     6541 aagctattca atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg
     6601 attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg
     6661 actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg
     6721 ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct
     6781 tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc agtcagcata
     6841 accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta
     6901 gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct
     6961 ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt
     7021 ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt
     7081 accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata
     7141 tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc
     7201 tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa
     7261 tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc
     7321 aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag
     7381 actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa
     7441 cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta
     7501 acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat
     7561 gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga
     7621 tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa
     7681 agataagtaa tatttcttca tattttggta ttttttactt ttattttttg ttttcattca
     7741 ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat
     7801 tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc atagccttct
     7861 tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg
     7921 tgtaagattt gggcacaaag accttcttcc atctttttga atactagttg ttctttcaag
     7981 tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag
     8041 tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc
     8101 aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa
     8161 tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga
     8221 caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag
     8281 aatggttttt taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct
     8341 ttacgccatt caattttttc catttttctt cctccgcgaa attccattta gttatttatt
     8401 atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct
     8461 agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc
     8521 ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg
     8581 taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg
     8641 tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga
     8701 cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt
     8761 tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt
     8821 ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc
     8881 atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg
     8941 atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc
     9001 ggtactttga caacggccat tgctccagca attggcccca cttatggagg cattatgact
     9061 tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat tctttctttg
     9121 attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg
     9181 ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg
     9241 ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac
     9301 tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca
     9361 ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt
     9421 gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg
     9481 atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat
     9541 caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta
     9601 gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt
     9661 tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg
     9721 aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc
     9781 gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat
     9841 aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg
     9901 cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt
     9961 ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag
    10021 gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt atgactctag
    10081 ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg
    10141 caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat
    10201 ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac
    10261 ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag
    10321 ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg
    10381 aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac
    10441 tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc
    10501 gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg
    10561 attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac
    10621 ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc
    10681 aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg
    10741 ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg
    10801 ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat
    10861 tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg
    10921 agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc
    10981 cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc
    11041 cttatatcgg ctatctatta gccaacttct taaatactgg tcattggtct acggaagatt
    11101 atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac agctttttca
    11161 agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt
    11221 tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc
    11281 ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat
    11341 aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa
    11401 atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg
    11461 tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg
    11521 tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac
    11581 ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat
    11641 tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt
    11701 cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga
    11761 tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc
    11821 catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca
    11881 cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt
    11941 gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa
    12001 cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa
    12061 tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac
    12121 tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc
    12181 aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg
    12241 ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt
    12301 cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt tgataagtct
    12361 ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa
    12421 atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta
    12481 cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct
    12541 cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc
    12601 ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt
    12661 cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat
    12721 aagctgtctt cctgtcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa
    12781 tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct
    12841 cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca
    12901 ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac
    12961 aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc
    13021 ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga
    13081 tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta
    13141 tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc
    13201 tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc
    13261 tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt
    13321 tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga
    13381 atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat
    13441 cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat
    13501 cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt
    13561 tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa
    13621 tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc tattaacttt
    13681 atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa aataacgaat
    13741 tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat aaaagccttg
    13801 ttgtataagc aaagtaatcg tttttctatg tttttttatt tttgaatgaa aaacgcagat
    13861 tctgtaacat tcactgcaat aatccataaa aaaaagcaaa aaaatttttg atttttatca
    13921 aattctctta aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca acaattactg
    13981 gacaaaaact ttgaataatg ttaactattt ttttatttta actttctccg caataatttc
    14041 tgggcatgtt ccaattcgtt tataacgact tcttctgttt ctttagctaa tatttcatct
    14101 aatacatcaa gccatttttc cggttctttt gttcctagac gtccaatagc ccaagctgct
    14161 gttcctctga tgacagggcg gacatctgtt aaacattctg aaatctcagg caatgccgtt
    14221 cgatcaccta gattagctaa agcaatcaat gcgtttctct gcaaaggttt cttcccacgc
    14281 catgaaccag ctaaatgccc aaattgctgt ttgaagtctt tattagacat cgaaagcatt
    14341 ggctttaatt ttggatatac ttcgtctact tttggttcca tctcttcatg aaaatgaaaa
    14401 tcttttcctt gattgtaagg gcagactaac tggcagatat cacatccata aatgacattt
    14461 cgcatttttt tacgatattc ttccggcatc atcccttttg tctgagtttg ataagacaaa
    14521 catcgctggg cattcattct tccgtcacca agcaatgctt ttgtcggaca agcagtcacg
    14581 cagcgcatac agtcaccgca accaaattct cctggtttat caggctcaaa tacaatatta
    14641 gtaacgatct caccaagata gacaaatgaa ccgaactctt cagtgatcaa caatccgttt
    14701 cgtccaataa atcctaaacc agctcgctgt gctacagcga catcgatcag ttctccagta
    14761 tctacttgcg gagctagacg ccattcctct tctttttgcc atttttctgc tcttgtttgg
    14821 atgaattcga tcaatcggtt caaacggtct ctgagtatat catggtaatc gatcccccaa
    14881 gaagcacgag caaactgccc gcgtttttca tccctaggca cttcttcatg gatcctagta
    14941 ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat caaatgtcat ttctggataa
    15001 agacgctcat cgatatttgg atgttcaaag ccagaagtat ggccagctgc tttttgttct
    15061 tccaacgatt cttttaatga atcaaaaggt tccgccgtgg tgaacccgat tttgtcaatt
    15121 cctaatcgtt tgctttcttg tatgatttct tcttttaatg tcatggtctt cctcgcttgc
    15181 tgttaacggc tgtataaccc ataatatagt ttttcttttt tcagctggac aaactggatc
    15241 tgctgatcgg caatcttctt ttcttccatc catttttttc ttgcagcaaa tacttcccaa
    15301 taatttgttg aaattaattt tcttttgtta tctaatgcat taaaaaaatt caatgaaact
    15361 ggttttgtac ttaacagttc atggagcctt acctcttccc atggcgaaaa ctcagacaga
    15421 tcgcgggaat acaatacttc ttcatagaga tttttttgct gtgtgtacgt gaatgtttgc
    15481 gggattttcc caggcctagc aatttctact agacgattat aaaacatatt cattaatttg
    15541 ctgttggcaa taaattgact tttataatag gccgactgga tctttttgat tactccttct
    15601 gctttgatga ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc gttttgttta
    15661 actaatacgc caggtgtgaa gccgactgga gaaaggaaca caaaataacc gataccggta
    15721 ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag ctcttcatct
    15781 ggttctgttg tctcattttg atttgctatg aataatttag ctaattctgc ttcaagaaat
    15841 tgaaggtctt gatccttgtc ggtattttcc tgctggctga tcaatcgaaa gacttcttct
    15901 ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca caccttcctt ccttactcta
    15961 actataacgg aatttcttca aaaaaggtat ctgtacgacg aaaaacagca taattttttt
    16021 tcatatctct tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc acttttcagc
    16081 tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat attccatttc atcatctgat
    16141 agtaagaatc tccttgttct ccttcttttg ctatggaatc agtgaacagt ttatcataga
    16201 ttttcaaacc cgtttctttt gctacccgtt ccatgcttct tgagtctaca ctggtttcca
    16261 cgaataaaac cggtacttct tttgctctta tctgatcaat gatcgctttc atttgatcag
    16321 gcgtaccttg actttctgta ttgatctccc atatataagc tgctggcaaa tcatatgctc
    16381 gtgaaaaata cttgaatgcc ccttcacttg ttactaataa ttttttgttc tcttcgatag
    16441 aagcaaaact ttccttcgct tgtgtatcta attcttttag tttttccaca tatgcttctg
    16501 catttttttt atagagtgtc gcattttctg catctttttc agagaatata cgtgcgattt
    16561 cctccacata ttttattccg ttagataggt ctagccaagc atggggatct gcttttgtat
    16621 gttcttcacc gctagttaaa taaagaggtt ctacattttt gctaactgca aaataatctt
    16681 ttccttcttc ttttttagcc gtttccatca agttatcgaa ccagctgtta cctgtttcaa
    16741 gattcaaacc gttgtataaa ataacatcag catcgcttgc ctttttgatg tcttctggta
    16801 gtacttcata ttcatgcgga tctgttccga ctggtacgat actgtggata tctatttgat
    16861 ctgttccgac ctcttttgcc atgtccgcca agatcgaatt ggtagccacg actgacaact
    16921 tttctttact tccttcatcc gcttggtcag aagcctgctt tccgcaaccg gctaatacca
    16981 agaaactaac tgccaagatt aaaaagagtg atttttttgt tttcatctat ctgcttcctt
    17041 tctgaaataa aattcctttt gttggagaaa ataaaaaagc aaggataaaa aatgcggtac
    17101 atgctaacac gatcgtagct cctgatggcc aattgtaact ataactgaaa aacacaccga
    17161 caactgcact tagcataccg attcctgcag ctgtaatgat cattttcagt aaatggttcg
    17221 ttagcaagta agccgtggcg gctggtgtga tcaacatggc aatcaccaag attgttccta
    17281 ctgtctgtaa actgactaca gccactaatg tcaaaaagaa catcaaaaga taatggatca
    17341 gcgatgtgtt cagcccataa gcttttgcca ttgtcggatc gaatgatgtg atcttcaatt
    17401 ctttataaaa gaaaaagaca aatatcaaga cgattccact aacgagtgct gtcagcaaca
    17461 aatcgctttc cctaacagct aagacattcc caaataaaat atgatacaaa tctgtcgcac
    17521 tctgggcata tgaaatcaag ataatcccta atgcaaaaaa ggagctaaag acaattccaa
    17581 tcgctgtatc atttttcaat cggctccgct gagtcacgaa accaatcact gctgctgtta
    17641 gcatcccaaa agcagtggct ccaaagatgt aactaaaacc aaacatataa gaaatggcta
    17701 cacctggaag aactgcgtga gagatagcgt ctcccatcaa agacatccct cgtaaaataa
    17761 taaaagaacc gatgactcca gaagcaagtc ctacaagcat ggaagtgacc aaagcgtatt
    17821 gcaggaattg ataattcatt aatccatcaa taaaggattg gatcattttt tctctcctcc
    17881 ttttactaaa tgtctgatga tctcaccata ggccaattga aggttcttcg atgtaaagac
    17941 ttcttgtact ggaccggaag cgatcagctg tttatttaaa agaataacct catcgaaata
    18001 ttcttctact ttatgaagat catgatggac gatcaaaatc gtttttcctg aattcttcaa
    18061 ttcctgcaag atgtcaaaga tctttcgttc acttaacgca tcaatcccta cgaatggttc
    18121 atctaaaaag atccattctg ctccttgggc tagagctctt gcaataaaaa ctctctgaag
    18181 ctgtccaccc gatagttcgc tgatctgtct tttggcatat tcttccatcc ctactttttt
    18241 caaagcttgt cttgcacgtt ctttttcttt tttcccaggt ctttgtccaa ttcgtaaaga
    18301 tggatatgtt cctaaaagta caacgcctaa tacatcaatt ggaaaggaaa gatccaattc
    18361 acttcgctgt tccacgtagg ctattttttt acggactgag tttaccggct gtccagaaaa
    18421 ggtgacttta ccagtagtag ctggtatcaa ctctagcaaa gatttcataa atgttgattt
    18481 tcctgcaccg tttggtccga taataccggt aattttttgt ggatttatcg tcaagttgat
    18541 agttttcaaa acagttttcc cttgataagc ggctgaaagg tcttgaatta caatggccgt
    18601 acgttccaaa tcttacactc ctttttaggt ttacctaact ttttatttaa atatagttta
    18661 actaaaatgg aaagcatttt caagtagaat gatgaaaaag tttttttcga attttcctcg
    18721 cattttatga aaattttctg tagaatcaca tcaagagttc aataaggcta acctaataat
    18781 gaaaaacatg tgttgaaacg tttgattatt ttttcttgtt gactcaacaa cgaaaggagt
    18841 ttttgtatgg aagaagaaaa gttaactcga agtcttagtg cccgtcatat ccaaatgatc
    18901 gcactaggtg gaacgatcgg tgtcggtctg tttatgggtg cctcctcaac catacgttgg
    18961 acagggccct cagtgatggg agcctatgcg attgcaggat tctttttgta tttgattatg
    19021 cgtgctttag gcgaaatgct ttacgttgat ccttcgactg gttcttttgc aaattacgcg
    19081 agcgagtaca tccatcctgt ggctggctac ttgaccgctt ggagtaatat ttttcaatat
    19141 attgtcgtag gaataagtga agtgattgct gtaggatctt atatgaatta ttggtggcct
    19201 gatttgccgg caattattcc cggaatcatt gtcgtcttgt ttcttatgct ggcaaatctt
    19261 atttctgtca aggcgtttgg cgaattagaa ttttggtttt cgatcatcaa agtcataacg
    19321 atcattttga tgatcattgc aggtcttggg gtaatcttat ttggatttgg taatcatggt
    19381 caggcagtag ggatttcaaa tctttggaaa aatggtggat tcttcactgg aggcgtcaaa
    19441 ggatttttct ttgcattatc gatcgttgtt gcttcctatc aaggaatcga actgatcgga
    19501 atgactgctg gtgaagcaga gaatcctaaa aagacgatca ttgaagctgt ccagtccact
    19561 atcggtcgaa tcttgatttt ttatattggt gctattttta tcattgtgag tatctatccg
    19621 tggaatcaat tgagtgagat tggttcacct tttgtacaaa ctttttctaa agtcggcatt
    19681 acatttgctg caggactgat taattttgtc gtgattacag cggcattatc aggttgcaat
    19741 tcagggattt tcagcgcaag tcgtatggtc tatacacttg ccataaatgg aaagatgtct
    19801 aaaaaatttc tgaaactatc tcgtcacggt gcgccttttt atcctgtcgt agcaatctca
    19861 ggtggaatct taattggtct gatcttaaat tatcttctac caatgttcat caaccagtca
    19921 gaaaatattt tcgttttcgt atacagctca agtattttgc ctggtatggt tccttgggta
    19981 gtgattttga tcagcgagat tcgcttcaaa aagaagcacg cagataaaat gcatgatcat
    20041 ccttttaaaa tgccactatc tccttatacg aattatgtga cacttgcttt tttagctatc
    20101 gtattgattt tcatgtttat caatcctgaa acgagaatct cattgattat tggactcgtt
    20161 ttcttgcttt acatgacgat ccattacttt gtacgcgaaa aaagaaccgg aaaataaaaa
    20221 aagaacgaag cttcacctta gtggaagctt cgttcttttt tattgctttt atcaattcaa
    20281 ttttagcgtc tcaccgggag tcaggaaata attctccatg ctcatcccat tcagcgacaa
    20341 gatcgtttct actggtacgc cagttcgttc ggctatttga tttggacctt ctccttccaa
    20401 aactacggtt gtcgcagcct gtgaagtcgc gctgttggta ttagtttcag aggaatctgt
    20461 tgtgtcttgt gttgttcgag cttgcatttc agaagcttct accgactctg ctgtctcaga
    20521 atcagaagat gttgtgtctt gtgtgcttgt tgactctgct tgaagagagg cgctagtctg
    20581 caaataaata tcggaaatct tagcgacatg attcgtataa ttagccgata attgattgat
    20641 caaagtatct aagtctacac cgtctttatt ttctgctgcc tgatacgctg cacgtaattt
    20701 tctgatccct tcgtcggaga ttgcttgaag ctcacgtgct ttttggttag caactgctga
    20761 taaaccagct attccctctt gatttccctg aatttctgtc tggtattctt caatcaatgc
    20821 aggcgtcttt tcttcgatct ttttcgaata atcatttacg attgcttgaa gttgctctgg
    20881 caaaccattt gctgtttttt cttcagaagt ttgtgtctct tttgttttag ttgatgactc
    20941 acttgtttgt ttcgtctctg ttttctttgt cgttgtttcc gttatggtcg tacttttcgc
    21001 ttctgacgaa ttttttccat gaaaatatag acccgcaact cctcctgcta tgatcaaaaa
    21061 cacggcaact gctgccccaa tcatgatcat ccgcttacga gctgcctgtt tttgctgtct
    21121 tcttcgggtc atccgaacat tttctcccca ctcattttca tcatacattt ttttcaccac
    21181 caatttttat gtataatagc gttatcaaac gcttcatatt cttattgtag caaataattg
    21241 tcgctgtgca catttttatc tttgttattt ataagaaaat ttcaccagtt gagatgattc
    21301 caagattgtc cgtcgatttc tccttgcagc tgcattgttc gttcgtctat ctcagccatc
    21361 agtttcttta tttcatataa gagcgcacgg tcttgatatt ctgatttttc gcttaattca
    21421 tctaattgtt ggatcagcca atcatttgtc ataatgaagt tcctttcatt tcttgcagtg
    21481 taaaggtcaa gtcttcgatc tgctcttttg cctcagtcag tgcttctacg atatatgctt
    21541 gtttttcaga ttcttcttcc tgcattttct gattttcctg tactctttga ataaaccagt
    21601 caatccgctg agtcagcgat ggtttgcctt ccaatgagag atactgctga taagcaatta
    21661 gcttctccca acgtttttcc tgggataaat acataaattg tgctaaaacg aatggcggaa
    21721 gataggtcat atggaattta tttgcgattc gctgatacgg cctcaaaaat tcggaaatcg
    21781 tatattcttc ttttcctcca gcttggtatt ctttttccgc cacacctgtt gtcaatacga
    21841 ttcctagttc tttccctttt aatacatgtg ctgctcgttc caagacatta tcttgccaaa
    21901 tttttaaatg agcaggagaa ctataccagt aaaacggaaa ctggaaaatg atccgatcat
    21961 gctgggccaa gagctgtagt tcagtctcta tatctatttt tccgtctgga taacttgatt
    22021 ccaaatgatg ccaagtagct ggtaaaccag cagaagcttc tttgaaaaat tgctgattta
    22081 aagattcttc gatcgttgga tggctaatga tgataagtgt acgcatccct tttcctcctt
    22141 tttagtcttc gctttacagt gtagcataac cgccacaagt ttcttttgcc gatgcttgtc
    22201 attttaacca gactaatcag gttgtccaag aaactgcggt ttgtttttcc aactgttttt
    22261 tcaaggtatc caaagcaata ttcttaaaat tttcgttttt catacgttta cgatagttcg
    22321 tagccgtttc tccggtactc tttttgaaca ttcggctaaa tacgactaga ctattgaacc
    22381 ctacttgata ggcaatactt tccacggatt catccgtcag agaaagaaga aacgcccctc
    22441 gattgatccg taccccgatc aaatattcct tgatactggt acccatatga tttttgaata
    22501 ctcgcgacaa atggctccga ttgaccgcta aatgctgggc gatttcctca acagacaatt
    22561 tttcagcata atgtgcctcg atgtatttga ctgtttcaac agctaaagag gaatgaacct
    22621 tttgtacacc accgctgacc tttccgccat cttttaaaag gactgataaa aaacggtagg
    22681 ttaatgctgt taactgtaat tcattttcca aggtttcttc cgtatctttc atgcattcta
    22741 aaataatgtc gatatatttt tgtatgtcag aagagaccat cgtgtattgg tcttctctga
    22801 aaagcgactg ccgaatgatt tcttctgcag cactcccacc aaatgaaagc cagcaataca
    22861 tccacggatc ttctccatct gataagtaaa acgtcgagtc tcctggacga atcaagaaca
    22921 gatcaccttt tttcaattgg aattgttgat ttttcacatg ataagatcct ttgccttcca
    22981 tcacgacatg caggatgtaa tcattgcgaa tagcaggacc aaaagaatga aacggcatcg
    23041 ttttagagaa accacaaaaa tcaaaataca attctttttc tacttgttga tgttcctttt
    23101 ttaaaaaaat cgattgctcc acgtctatcc ctccttactc ctttagtata atgcaacaaa
    23161 aagttaaata aaagtcacag aaatatatta aaaaggaagc gatttcttgt taagtttaag
    23221 tcattaaaag atctttgtta ttcgttgtaa ccggtttact tagtattata tattgaacag
    23281 gagaggaata cagattatga aaagcaaaat gatccaacgt gtgtcctacg ctttaggcgc
    23341 ttttggacac gacacattct atgttacatt aagcacttat tttatggtat tcgtgacaag
    23401 ccagatgttc gcaggaacag ataaggcaac gaatgcaaaa atgattgcta cagttacaag
    23461 cttagttgta ggtatccgct tagttgaaat tatttttgat cctttgattg gtggcatcat
    23521 cgataatact cgtacaaaat acggtaaatt caaaccatgg ttagttatcg gcggcgttgt
    23581 tagttccgta ctcttggtag caatctttac caatttcttt ggcttagcta ctcataatac
    23641 gactttattt atcatattat ttgcaatcgt tttcatctta ttggatagtt tttattcatt
    23701 taaggatatc gctttttggt caatgattcc agctttgagt acggaaacaa aggaacgtga
    23761 aaaatttgct acgtttgctc gtttcggctc ttctctaggt gcaaatggta ctactctagt
    23821 cgttgtccct atcgttactt tctttaccta tcttgttact cataaacata ttgaaggcgc
    23881 ttcaggctgg ttctggttcg gtgtgattgc tgctgttatt tcaggcggta ctgcgattat
    23941 cactgccttg ggcacaaaag aagtcgatac tgcgatccgt cagcaaaatg aaaaagctgg
    24001 gatcaaagat gtgttcaaag cagttgctca aaatgatcag ttgatgtggc ttgcccttgc
    24061 ttatatcgct tatgcgattg ccaatgtagc aacaactgcc gtattattct acttcttcaa
    24121 atatgtactt ggccaaccaa ctgaattttg gatcgttggt ctagttgctg ccttacttgg
    24181 tttagtagct gttccactat tcccaatgct tacacgcgtc atctctcgtc gctacgttta
    24241 ccttggaggg atcgcaatga tgatttcttc ttactttttc tttacgatcg caggttcaag
    24301 tttgccaatc gttatcatcg ggctagtatt gttctacttc cctcaacaat tgattttctt
    24361 atccgctttg atgacgatca cagactctgt tgaatatggt caatggaaaa atggtatccg
    24421 taacgaagct gttactctat caattcgccc tttactagat aaaatcgctg gtgctttgtc
    24481 taacgggatc gtagggtttg tcgctatcgc ttctgggatg actggtaatg ctactgctgc
    24541 tgatatcact gctcatggcg taacgacttt caaagcttat gcattctatg ctcctgctgc
    24601 tttgatgatt ttctccgcta ttatttttgc ttggaagatt acattgacag agaaaaaaca
    24661 tgcacaaatc gttgaggaat tggaaaacaa attggctcca gctgatacaa tggaagatga
    24721 acttcacgat gcagtagttc cttcccatta agaaaggatc ttgaaaatga agattacaga
    24781 aaaagatttt ggccaaggtt atcacttgat aaccttggag aacaaaaaca agctagcact
    24841 ttctatctct gatttaggtg ctagaatcgt cagtttaaaa tcaaatgatc gagaattagt
    24901 tttgggattt gacaccgctg aagaatatat cgaaaaagat ccgtatatcg gtgcttctat
    24961 cggtcgcacc gcagggagaa tcgaaaatgg ccgcttttct ctcaatggaa aaacttacca
    25021 attagccaca gatccaaaaa ctggacataa tctgcatggt ggtgctcctg gatttgaatt
    25081 aaaaaaatgg tcttatgtca ttttgaatgg cgaaaatgaa gcttctgtca tttttactac
    25141 tacaagtccc gacggagaac atggttttcc aggtacaatg gatgtggaaa tccgctatac
    25201 tttgacaaaa gataatatct ggcgagtgac ctctcgcggc accagtgacc aagatacttt
    25261 attcaatccg acaaatcatg tttacttcaa cttgacggga gatgcttctc agtcaatcga
    25321 tcaacatgag ctttggctca atagcgaagc ttatgctcct ttgcgaacag atagtattcc
    25381 tataggggta aaagaaaatg ctgcaggaag tgctttcgat tttcaaatac ctaaaaaatt
    25441 agcgtccgtt tttgctagtg atttggatca aaaaaatctt gttgacggaa tcgatcatcc
    25501 atttttctta aaagaaaccg gcttgggtaa agaagccgct cgcttgacta gccccgataa
    25561 aagaatccaa gtaggtattg ctacagatgc ttctagtgtg gttattttca ctgcaaattt
    25621 cggcacagaa acaccagaaa tgcgcaaccg aaaactggca catcacggtg gcatcacatt
    25681 tgagactcaa acagctccag gtgcagagcg cttctcagca tttggttcga ttcatttaaa
    25741 agctggatca gtatttgaaa cagtgacaga attcaaaatc aaaacaagaa aggaataact
    25801 catcatggaa atcaaaagaa gcttaaacac aaaatttgcc gaaacattcg gacctaaaaa
    25861 aactacacaa tacttctcac caggacgcat caatttgatt ggtgaacaca cagattacaa
    25921 tggaggccat gtcttccccg cttccatcac ttatggaacg caaggtgtag cagcacctcg
    25981 agacgataat aaaatattag tttattccac aaactttgac gatatcggtg tcattagttt
    26041 tactttagat gagctagaat atgataaaaa agcaaactgg gcaaattacg taaaaggaat
    26101 ggtccttaaa ttaaaagaag ctggacactc aatcgatcat gggtttgagt tgttagtgga
    26161 aggaacaatc cctaatggtg caggactttc cagtagcgct tcattagaat tattagtagg
    26221 tgttgtatta gaagatctgt ttgatttaaa aacagatcgc ttggaactag tccaaacagg
    26281 taaaaaagta gaaaatgaat tcatcggtgt caattcaggt atcatggatc agtttgccat
    26341 cggcttcggt gagctagatc atgcaatctt attagataca aatacgctga aatacgaaat
    26401 ggttccagtg aaactagatg gttatgcaat cgtcatcatg aatacgaata agcggcggga
    26461 attggcagat tcaaaataca atgaacgccg tagcgaatgc gaagaagcct tggcacgttt
    26521 gcaaaaaact ttgcctatct cttctttagg agacttagat gaagaagaat ttgaatcaac
    26581 tattgaccaa atcggcgatg atacattgat acgcagagct cgccatgccg tttctgaaaa
    26641 tcagcggact ttgaaggcaa aagctgaatt agaagctggg aacttagaag catttggtca
    26701 actgttaaat gattctcacc actctcttcg ctatgattat gaagtaactg ggatcgaatt
    26761 agatacacta gtagatgctg ctcaaaaaca agaaggtgtc ttaggtgcac gaatgacagg
    26821 agctggattt ggtggctgtg cgattgctct tgtcaaagaa gaaaatatac ctgccttcaa
    26881 aaacaatgtc tatgatgaat acatgaaagt tgtcggctat gctccggaat tttatgttgc
    26941 acacattgga aatgggacaa caaagatcga tccagaagct gttcatgttt gataaaaaaa
    27001 tcaacgatct cagctaaaaa caaatagata aaaataacct ctcagcaatt tccaaatgtg
    27061 agccttttgc tcgtatttga aaattgctgt tttttattga cacggaaaga gaacgtgtgt
    27121 tctaattgtg ttgaagaggt gattgttatg tgggcgaatc aaaatccggt atttgtttat
    27181 gaaaaagaac cttcaagaga tattttatgt attgactgca aatcttttta cgcctccgta
    27241 gagtgcgttg aaaaggatct tgatcccctt acaactaaat tagtagtcat gtcctatccg
    27301 tccgattcga cagaaacacg gggtagcgga ttaatcttgg cttccagtcc tgcagcaaaa
    27361 aaagcttacg gcatcaccaa tatcagccgt gccagagatc ttcccttccc ctatccaagt
    27421 gatttatata ttgttgcccc aaggatggcc tattatatgg aaaagaacag agaaatcaat
    27481 tctatctata aaaaatatgc agacgaagcg aaccatcatg tctattccgt cgatgaaagc
    27541 ttccttgatg taacaaatag catggcactc tttggtaaaa caaatgcgta cgacatggct
    27601 aaactgatcc ataaagatgt gtttgagcag accggaatct acacgactat aggaattggc
    27661 gataacccgc tacttgctaa actagcctta gacaatggct caaaagacgg aaagcaaatg
    27721 tttgctgaat ggcgttatga agatgtacca gaaaagttat ggaaaatctc agatatcact
    27781 gacttttggg gaatcggcaa acgaacagca aaacgtctga aacaaatggg tatacattct
    27841 gtttatgatt tagctcacgc ggattattat cacttgaaaa gcaagctagg ggtcatcggt
    27901 actcagcttt atgcccatgc ttggggcatc gatcgaagtt ttttgggaca agaatataca
    27961 ccaaaatcaa aaagtatcgg aaatagtcaa atcctagata gagattatac aagacgagaa
    28021 gaaatcgaga tcgttatcaa agaaatggcc gaccaagtcg gcactcgctt gagacgatct
    28081 gcagcaaaaa cacaagtagt cagcttatgg gtaggatact ctttaggcta tcttgatcca
    28141 aatggcaaga ccgggtttca ccagcaaatg aaaatcgaag cgacaaatgc tagcagagat
    28201 ctagcagatg ctttattgac cattttccac cagcattacc actaccagga tattcggcgt
    28261 gtaggcgtga actgttccga cctcgtatac gcgacaggtt tacagcttaa cctttttgaa
    28321 gatcctgaaa agcagataaa tgaagcgaaa gttgattttg ttgtcgatac gattagaaaa
    28381 aaatacggct ttcaatcaat catccatgcg catagtctct tgcaaggagg acgcgccatc
    28441 gcacgtagtt ctctcgttgg cgggcatgca ggtggtatga gtggattaga aggtggaaaa
    28501 gatgagacgg acagcaaaaa aattcattga ctataacgag tatcgagatc ggccatttgg
    28561 attaaaatgg gggactgctt ttgcgatgga agaactagtc aacggtatca aaaaaaatga
    28621 agaagaagca cgcaaacagc ctcttcctaa acaacaaatg acattgcaag aaatcgatag
    28681 tatactgaca gaagcctttt tatctcgtaa agcagtaatc attcagctca atttaaaaga
    28741 tgaatttgga agactcttgg atgaggtcga agggcacttc attggtgaag cctatgaaga
    28801 ttactttatg attgaccatc agccgatatt gtgggaagac gtacggtata ttgagttaaa
    28861 ggaaacaaaa aaatggtttg tgacatgaga ggttgtgaca gaagtgatca acttcaaaaa
    28921 ataagaaagg atttttcgaa aattgcttct taaatttttg tggaattttg gcttattttt
    28981 cgaagaagca acttctggaa caacgtatat tagatatcag aggttgtgaa aaagtcgctt
    29041 agttttgagt aacaagccaa ggcgctgatt tcacaacctc tgtctatcta tccatctatt
    29101 cggtaagatt catgcatgac gtcttcttta tttagtatgt agagcccttt ttcttctgac
    29161 cattcaacat aaatcagctg ttttacatct gcatagcctt ctttcttcaa gttctccata
    29221 agccatgctt cagtcaattg attggactga agcactttat gctgaatttg cccttcatct
    29281 acaagcagga tcgataaagc tttgtcttca tctgattttt tcaatacact catttgacca
    29341 gcattctcta ggataacatg tttggcattt tcaagagaaa agcattcttg ctgtcttaag
    29401 attgctcgca actgctccat ctcgatatgg ttatttgtca actctgtcat gttgatgacg
    29461 ccgtctttga ttagaacaga tggctcgcct ttgacccaac gattgacctt ttcatttttt
    29521 tggacgatac gttcgatacc ataaatcatt actgcccaaa gcgcaatggc aaataggaca
    29581 tgccctacat ggacattgtc atcataggtt gattcttcta agatcccacc taaaacaagc
    29641 gtatagacta aatcaaacgg tgtgatttct gacatggatt tcttcccaag caaacgtacc
    29701 actaataata aaccgatcaa cccgaacacc agtttcattg taattgaaaa aatcattttt
    29761 tctcccccta actatctgtt aattaaatta tatatgcttt ctcacgaaca cgcatggaat
    29821 atacaaggaa gaaatgtatc cttttactaa aaaaaggact gtgacactgt agtgaccccc
    29881 aaaagttaga cttttttgga gtggagaaat ccactccttt tttttatgct ctaaaatgat
    29941 atttttacac aaccgttacg gttgttttac gaaattggac g
//
LOCUS       DAJSBN010000031        28197 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJSBN010000031 DAJSBN010000000
VERSION     DAJSBN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.031"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            160..1302
                     /locus_tag="ONT86_001605"
     CDS             160..1302
                     /locus_tag="ONT86_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR2352405.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            1318..2529
                     /gene="thiI"
                     /locus_tag="ONT86_001606"
     CDS             1318..2529
                     /gene="thiI"
                     /locus_tag="ONT86_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR2352406.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            3102..3749
                     /locus_tag="ONT86_001607"
     CDS             3102..3749
                     /locus_tag="ONT86_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR2352407.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    3878..4094
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4142..6787
                     /locus_tag="ONT86_001608"
     CDS             4142..6787
                     /locus_tag="ONT86_001608"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR2352408.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            7097..8410
                     /locus_tag="ONT86_001609"
     CDS             7097..8410
                     /locus_tag="ONT86_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR2352409.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            8400..9053
                     /locus_tag="ONT86_001610"
     CDS             8400..9053
                     /locus_tag="ONT86_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2352410.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            9108..9659
                     /gene="radC"
                     /locus_tag="ONT86_001611"
                     /pseudo
     CDS             9108..9659
                     /gene="radC"
                     /locus_tag="ONT86_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            9790..10875
                     /locus_tag="ONT86_001612"
     CDS             9790..10875
                     /locus_tag="ONT86_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2352411.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            10872..12764
                     /locus_tag="ONT86_001613"
     CDS             10872..12764
                     /locus_tag="ONT86_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2352412.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            12771..13148
                     /locus_tag="ONT86_001614"
     CDS             12771..13148
                     /locus_tag="ONT86_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2352413.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            13302..14084
                     /locus_tag="ONT86_001615"
     CDS             13302..14084
                     /locus_tag="ONT86_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HCR2352414.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(14210..14941)
                     /gene="erm(A)"
                     /locus_tag="ONT86_001616"
     CDS             complement(14210..14941)
                     /gene="erm(A)"
                     /locus_tag="ONT86_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HCR2352415.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(14999..15058)
                     /locus_tag="ONT86_001617"
     CDS             complement(14999..15058)
                     /locus_tag="ONT86_001617"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HCR2352416.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            15450..16112
                     /locus_tag="ONT86_001618"
     CDS             15450..16112
                     /locus_tag="ONT86_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2352417.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            16347..16490
                     /locus_tag="ONT86_001619"
                     /pseudo
     CDS             16347..16490
                     /locus_tag="ONT86_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            16640..16840
                     /locus_tag="ONT86_001620"
     CDS             16640..16840
                     /locus_tag="ONT86_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2352418.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(17094..18773)
                     /locus_tag="ONT86_001621"
     CDS             complement(17094..18773)
                     /locus_tag="ONT86_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR2352419.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(18775..18987)
                     /locus_tag="ONT86_001622"
     CDS             complement(18775..18987)
                     /locus_tag="ONT86_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCR2352420.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            19380..21110
                     /gene="efrA"
                     /locus_tag="ONT86_001623"
     CDS             19380..21110
                     /gene="efrA"
                     /locus_tag="ONT86_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCR2352421.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            21107..22867
                     /gene="efrB"
                     /locus_tag="ONT86_001624"
     CDS             21107..22867
                     /gene="efrB"
                     /locus_tag="ONT86_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCR2352422.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            22962..23159
                     /locus_tag="ONT86_001625"
     CDS             22962..23159
                     /locus_tag="ONT86_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352423.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            23312..23797
                     /locus_tag="ONT86_001626"
     CDS             23312..23797
                     /locus_tag="ONT86_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR2352424.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(23901..24494)
                     /gene="clpP"
                     /locus_tag="ONT86_001627"
     CDS             complement(23901..24494)
                     /gene="clpP"
                     /locus_tag="ONT86_001627"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR2352425.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            24674..25201
                     /locus_tag="ONT86_001628"
     CDS             24674..25201
                     /locus_tag="ONT86_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352426.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            25292..26029
                     /locus_tag="ONT86_001629"
     CDS             25292..26029
                     /locus_tag="ONT86_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR2352427.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            26033..26950
                     /gene="miaA"
                     /locus_tag="ONT86_001630"
     CDS             26033..26950
                     /gene="miaA"
                     /locus_tag="ONT86_001630"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR2352428.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            26952..28181
                     /gene="hflX"
                     /locus_tag="ONT86_001631"
     CDS             26952..28181
                     /gene="hflX"
                     /locus_tag="ONT86_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR2352429.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         9420 a         4609 c         5934 g         8234 t
ORIGIN      
        1 aaaagttagt aaaccagcaa gagcaagacg gattattgtc ttgttcttgt ctttttttgt
       61 atatttgaaa attgaaacaa gtgaaaattt cacctgctct tgaaagcacc ttggacaacc
      121 gttataatag aaaagaattt caaaaaagag gggtttctta tgatatattt tgataacagt
      181 gcaacaaccc caatctttcc gcaaagctta gatgcatatg taaaaaccag tcagcggatt
      241 attgggaatc catcaagctt gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa
      301 gcaagaaagc agatcgctga tttgatccat gtagaaccag aagaaatcta ttttacttca
      361 ggcggaacag aaggagataa ctgggtatta aaaggaacga tgatcgaaaa aagagaatat
      421 gggaatcata tgatcatttc aggagtcgaa catccggccg tcagcgagac agcagaacaa
      481 ttaaaagaat taggaatcga agtaagtatc gccccagtgg ataaacgcgg atttgtacgt
      541 gtcgacgaat tgaaagaatt aataagaaaa gagacagtat tagtctctgt tatggctgtg
      601 aataatgaag taggatcgat tcagcccatc caggaaatca gtgatctttt ggatgatttt
      661 cctaaaattc atttccatgt agatgctgtt caggcgattg gaaaagtacc attctcgcaa
      721 tggttgacag aaagagttga ttttgcaaca ttttcagctc ataaattcca tggcccacgg
      781 ggaactggtt tcatttattg gaaaaagggt cgtcgtttag caccattact gacaggtgga
      841 gggcaagaaa gaaatcagcg aagtgggaca gaaaatgttc caggtattgt ggctatggtt
      901 aaagctttac gtttatcatt agagataaag gaacaaaaac ctgagcagac acaacgctta
      961 aaagtagctc taatcaatgc gttgaagacg tatgagaaag tgacgatttt ttcagaagga
     1021 actgactatg caccgcatat tttatgtttt gctctaaaag gaattcgcgg cgaagttctc
     1081 gtccatgcct tagaagaaaa acaaatattt gtttctacta ctagtgcctg ctcctcacgt
     1141 aaacatatgg caagcagtac attgcacgca atgcatgtac caaatgatct ggctacatct
     1201 gctgttcgaa tcagcttaga tgaaaacaat tcgcttgcag aagtcgagca atttatgatt
     1261 attttcaagc agctttataa gaaattttct gcaatcaatt aaaagaggag tgttcttgtg
     1321 aaatacagtg aaattatggt ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa
     1381 agctttatca tgcaactagc acaaaatgtc agacaagctt tgaaagactt tccagaaatc
     1441 aaaatccatg ctgatcggga ccggatgcat cttttattga atggcgcaga cgggcagcta
     1501 gttattccaa aactagcaaa aatatttggg atccaaaatt tctctccaag tattcgtgta
     1561 gaaaaagatg tcgaagtttt gaaacaagct gttcaagaca tcatgaaaga aatttatact
     1621 gggaaagaaa catttaaaat tatcgctaaa agaagcgatc accaatttga attgacaagt
     1681 aatgaattga atcaaacatt aggtaatgct gtttttgata ttttcccgca tatccaagtt
     1741 caaatgcgtc aaccagatat tccattacgt gtagagattc ggcgtgatgg cgcttatcta
     1801 tcgtatgaaa cgatcaaggg agctggagga ttgcccgtag gaacaagcgg aaaaggaatg
     1861 cttatgcttt ctggggggat tgattctcct gtagcagggt actttgccat gaagcgggga
     1921 gtggaaattg aagctgttca ttttgctagt ccgccgtata caagtgaaca agcactgcaa
     1981 aaagcaaaag atctaacagc caaactagca ccttatgtgg gaacgatcca ttttatcgaa
     2041 gtgccattta ccgagattca agaagaaatc aaaaagtcag tgccacaagg atattggatg
     2101 acaattactc gtcgaatgat gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt
     2161 ttagtcattt tgaatggtga atcattaggt caagtggctt cacaaacttt gcaaagtatg
     2221 gtggctatca atgaagtaac gaatacgcca atcattcggc ctgtagcgac aatggataaa
     2281 cttgaaatca ttgaagtagc agaacagatc gatacattta atctttctat ccagcctttt
     2341 gaagattgct gtacgatttt tgctcctcca cagccaaaaa cacgccctca actggaaaag
     2401 gtccatcaat atgaagagcg tttagcaata gatgaaatga tccaacgagc actagctggc
     2461 ttgaagatgg aagagataca agcagaatcg tctaatgaaa aaactgaaga attttcagaa
     2521 ttgctataag aggttatgaa aatagtgttt tgaccagagt attagagaaa caaaacgaaa
     2581 aaatagctcc atgtatttta ccgtttttga actaatgtcg aagattagct gattgaatac
     2641 catttatcaa gaagttatgg aaaagccggt tcttttttac taactgaaaa ataaaatttt
     2701 atttagagat caagctacag ttgataaatg atataaaaaa gagaatttcc catgaaatag
     2761 cgaggttgtg acagaagtgg gcagcttcaa gaaataagaa ggaatttacg aaaattgctt
     2821 ttcaaatttt tgtgaaattt cggcttattt ttcgaagaag ttacttctgg aacaccgtgt
     2881 atcagaggtt gcgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag
     2941 ttcctcatat tttacgtttg gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt
     3001 tttatttgtc tcacaccctt ttccttccta cttgtaacct tgctcccgtg ttagaaacat
     3061 gttacactaa atatgtgaaa ttattaacaa ggagcgtcgt tatgcgagaa aaaacagata
     3121 cactgccaaa agcaactgcg aaaagattac ctttatattt acgctacttg aagatgctgg
     3181 atgactcagg tataagtaga atcaaatcaa atgagttcag cgaaatcacc caaattcctt
     3241 ctgcaacgat ccgcagagac ttctcgcaat taggcgagct aggcagaagc ggatatggct
     3301 atgatgtccc ttttcttatc gatgtattca ataatatcct taatactaaa gaagaaaaac
     3361 gaatcgcatt agtaggatat ggaaatcttg gcaaagcatt gaaacacaac aattttagaa
     3421 gaaatgaaaa tctaaatatc gtgtgcgtct ttgataatga tccagcactg atcaatcgag
     3481 taattgatgg agaaatgatc tatccgattg atcgtttcgc ggagatagcc aaagcaaaaa
     3541 atgtgacagt agcaatctct acggtaccaa gtaaatattc acaatctgct attgatgaaa
     3601 ttgtcaaagg aaatgtaacc gctatactta attttgcgcc tgacagagta actgtcccag
     3661 cttatgtcaa tgttcaatac attgatttga caaccgagtt gcagacgctt atttactttg
     3721 atgagaatta ctcagaagtt ttttcttagg aaaatttgag taggcttatt gatcttgcga
     3781 gaactcgtcg taattctttc aaaaattttt tagtactaaa tgaagtggtt ttttcacgac
     3841 cacttgttga ctaaaaaaaa aaaccattgt agaatgaagc ataacgctga acaagaggag
     3901 taactggcac attcattctt agagagagaa ctatctgctg aaaggttctc aatgacaacc
     3961 agtgaaggta gcttgggagt ttctgtcttg aatggagtag agatagacga gtcgtgatcg
     4021 ttaactcact tgagaggcaa taatgattga ttattgcaat ttaggtggta ccgcgtaaat
     4081 catacgtcct agaatacgaa agtattttag gacgtttttt agtttaaagg aggaaaacag
     4141 catgacggaa gaaaataacc tatcaacaaa atataatccg caagaagttg aagctggacg
     4201 ttatcagcat tggttagaac aagatttgtt taaaccgagc ggagataaaa aagcaaaacc
     4261 ttattcaatc gtaatcccgc ccccaaatgt tactggaaaa cttcatttag ggcatgcttg
     4321 ggatacgacc ttgcaagata tgctgattcg tcaaaagaga atgcaaggat atgatacttt
     4381 atggctgcct ggtatggacc atgcaggaat cgcgacacaa gcaaaagtag aagcgaagct
     4441 tagagagcaa gggatttctc gttatgatct aggtcgtgaa aagttcatcg atcaagtgtg
     4501 ggaatggaaa gaagagtatg cgagccacat ccgcgaacag tgggctaaaa tgggcttatc
     4561 tttagattac agtagggaac gttttacatt agatgatgga ttatctgaag cagtacgtaa
     4621 agtttttgtt accttatacg aaaaaggttt gatttatcgt ggtgaatata tcatcaactg
     4681 ggatcctcaa gcacgaacag ctctatctga tatcgaagtt attcataaag atatcgaagg
     4741 cgccttttat catatgagct atgaactagc agatggatca ggcgtagtag aaattgcgac
     4801 aacccgtcca gaaacgatgt tgggagatac ggcgattgct gttcatccag aagacgagcg
     4861 ttatcaagcg ctcatcggca aaaaagtgat tctaccttta gtcaataaac aaatcccaat
     4921 cattgcagat gaatatgtag atatggaatt cgggacaggt gtagtaaaaa tcactccagc
     4981 acatgatcca aacgactttg aggtaggtaa tcgtcatgat cttccacgcg taaatgttat
     5041 ggatgatgat ggtacgatga atgaactagc aggaaaatat gctggaatgg aacgttttgc
     5101 tgctcgtaaa gcaatcgtgc aagatctgaa agaaatcggt cgattgatta aaatcgatac
     5161 gatgatccac agtgtgggtc attccgagcg gacaggtgtc gttgttgaac ctcggctgtc
     5221 tactcaatgg tttgtaaaaa tggcgccgtt agcgaaaaaa gccattgata accaagaaac
     5281 agatcaagca gtggaattct tccctccaag attcaatcaa acgtttttac gttggatgga
     5341 aaatgtccat gattgggtaa tctcacgaca attatggtgg ggacatcaaa ttcctgcatg
     5401 gtatcacaaa gaaacaggtg aaatgtatgt agggatggaa gcaccagcgg atagtgaaaa
     5461 ctgggtacaa gatcctgatg ttttagacac ttggtttagt tcagctttgt ggcctttctc
     5521 aacaatgggc tggccagata cggaaagcga agattataag cgatacttcc ctactagcac
     5581 tttagtaacc ggttatgaca tcattttctt ctgggtaagc agaatgattt tccaaagctt
     5641 ggaatttact ggcgaacgtc catttgagaa tgtcttgatt catggattga ttcgtgacga
     5701 acaagggcgg aagatgagta aatcattagg taatggaatc gatccgatgg aagtaatcga
     5761 aaagtatgga gcagatgctc tccgctggtt cttatcgaat ggttctgcac caggacaaga
     5821 tgtccgcttc agctatgaaa aaatggatgc ttcttggaac tttatcaaca aaatctggaa
     5881 tgcttcacgt ttcgttatca tgaatatcga aggaatgacc gtagaagaga tcgatttatc
     5941 tggagaaaaa tcagtggctg accggtggat ccttactcgt ttgaatgaaa cgatcgaacg
     6001 agtaactgag ctatttgatc gttttgaatt tggtgaagca ggtcgacaac tgtataactt
     6061 tatctgggat gatttttgtg actggtacat cgaaatgagt aaagaagtac tttatggaga
     6121 tgatgctgtt tcaaaacaaa ccacccgtag catcttagtc catacacttg atcagatcct
     6181 acgtttattg catccaatca tgccattcgt tacagaagaa atatgggaac atattcctca
     6241 tcagggaaat tctctagtag tggcagaata tcctgttgta cgtccagagt ttgatgatga
     6301 aacagcttct aaagggatgg aagtattgaa agaattgatt cgagccgttc gaaatattcg
     6361 ttcagaagtg aatacaccat tgtctaaacc aattacgttg ctaattaaaa caaacgatgc
     6421 taaaatcgat caatttttag ttgaaaatac aagctatatc gaacgcttct gtaatcccga
     6481 agagttgacg atatcaagtg agatcactgc gccagatcta gcgatgtcag ctgttctgac
     6541 aggtgcagaa atcttcttgc cgttagcagg attgatcaat attgaagaag aaatcaaacg
     6601 attagaaaaa gaactggaaa agtggacaag cgaagtaaaa cgtgtccaag gaaaactttc
     6661 taatgaacgt tttgtttcta acgcgccgga agaagtagta gaggcagagc gtgcaaaaga
     6721 aaaagattat ttagagaagc aaacggctgt caaagaacga atcgaaagtt tgcgtactat
     6781 tcaataagaa tagaaaaaga gagtgtgaaa tcagctagct attactaaaa gctagctgat
     6841 tgaacatcgt tacaggaaag agtttgagcc tcactcttag cttcgattca taaggagatg
     6901 tgacataaat tttgtcgcat ctctttttta gtctactctt attatgtagg agaaggaggg
     6961 aggtggatca tgtatcaaga gtcaataaag tagttgataa aatcgttcct ctctctgttt
     7021 cttttctctt tgtcatactt ttatttttct tatgtgttct atttgttata ataaaagaga
     7081 aatgggggaa ttgttgatga cgattgaaga agcaatagaa tggatccata gtcgattacc
     7141 atttggttca cgtcctggcc ttgaccgagt cgaagcttta ttagaagtgg tagggcatcc
     7201 agagagaaaa gtaaaaacga tccatatcgc tggaacaaac ggaaaaggtt ctactgtagc
     7261 ttatttgaga agcctattag aagaggctgg gctgacagta ggtacattta cttcacctta
     7321 tatcgaaacg ttcaatgaac ggatcgcaat caatggcaaa agcattccag acaatgattt
     7381 gatccagctt gtaaaaaaat atcagccact gatcaaacaa cttgatcaat ttgaagcagt
     7441 agctggtatt actgaattcg agacattaac agcaatggca tttgattatt ttttgatgaa
     7501 gaaagtggac gtagcgatta tcgaagttgg cttaggcggc ttattggatt ctaccaatgt
     7561 agctgtacca atgctgacgg gaataacaac gattggactt gatcataccg aaatacttgg
     7621 agatacaatt gaagaaatcg ctgcacaaaa agctggaatc atcaaacaag gcgttccagt
     7681 agtaacagga aatattgccc cagaagcttt tactgtcatc caacaaacag ctaaagcaaa
     7741 aaatgcaccg ctttatgtat acggggaaaa ttataaaata aactatctgc atcctgatga
     7801 gatgtgggga gaagtatttg aattttttaa tgaaatagga aagatccctc aattaaccac
     7861 tccgctttta gggcaacatc aagctgaaaa tgcaggagta gccatcgaat tgtattttgt
     7921 ttactgtcaa ttggaaaagc tgcctttccg agaaaaagat gttttgaagg gattgaagaa
     7981 atctcgatgg ccaggaagaa tggagcgttt gagtaacgag ccattagttg ttttagatgg
     8041 tgcccataat acgcatgcaa tgaaccggct tgttgagaat ttgaaaaaag aattcaaagg
     8101 atatcaaatc aacattttgt tttccgcctt acgaactaaa aatgttagag aaatgctaga
     8161 acgattgcag caagtaccaa atgtccatat ttatttgacg acctttgaac atccaaaagc
     8221 catcgaatta agtgaagtat cagatttagc aagtgagaga gtatcaatcg tttcattgtg
     8281 gcaattcgga ttagcagata tattggaaaa gatgacaaat gaagacgtat tagttgtgac
     8341 cggttcactt tatttcattt cagaagttcg ccatttatta atacaattag gaggagatca
     8401 tgaagtataa tgcagtgatt tttgacatgg atggtttgct gttcgacaca gagttagtgt
     8461 attacgaagc cacacaagta gtagcagaca agatgggttt tccttatgat aaagacctgt
     8521 atttaaaatt cttaggtgtt tcagatgaag aagtatgggc gaattatcat atgattttct
     8581 cggaatttgg caaaacaagt gtacaaagat tcatagatga ttcataccag gaaacgcttg
     8641 aacgtttttc gcaaggagcg gtacaaatga agcccggtgt gttggaactc ctctcttttt
     8701 tagaagaaca tcagattccc aaagttgtcg cttccagtaa ccaacgagca gtcattgaac
     8761 ttttattgaa gaagaacaat ctgtttgaac gatttgagac gattgtttct gcagaaaatg
     8821 ttcaacgagc aaaaccagac ccagaaattt tcttgttagc taatcagaag ctaggtacag
     8881 cgaagaaaga aacattgatt ttagaagatt cacaaaatgg tattttagca gcttctgcgg
     8941 ctgagatccc ggtagtcatg gtaccagatc tgcttccacc atcccaagaa ttgatcaaaa
     9001 aaacagctgg ggttgtttct tctttacatg aaatccctgc attgttaaaa taaaaatcca
     9061 tctcttttcg tatcttattt tagataagat agaaaggaga tgtttttttg ggaaaacgat
     9121 tgatcaaaga agtacctact agctctttac caagggaacg tatggaaatt tatggcgcgg
     9181 aagctttatc ggatcaggag cttttggcaa tcttactgcg taccggtcaa cacccttata
     9241 gtgtgatgtc gattgctgga aatctgttaa aaacgtttgg aggactggcc tctttacgac
     9301 aggctacttt gcatgagtta gaagagattc gagggatagg acgcgtgaaa gcaatcgaaa
     9361 tcaaagcctt gatcgaatta ggtcgacgaa ttcagacgga tcatgaaaga gcggctccac
     9421 cggttcgtac cagctacgaa ttggcccagc aattgattct ggaaatgaaa gatcacaaac
     9481 aagaacattt ggtttgtatt tatttggaca cgaagaatca agtgatctta aaaaaaacag
     9541 tgttcatcgg ttcgctgaat cagtcagtcg ctcatcctag agaaattttc cattatgctg
     9601 tacgttattg tgcagcaagg atcgttttag cgcataatca tccttctggt aatgttttaa
     9661 ccatagtata ataaataata gtagttaaag tagtttaaga ggagatatta aagggatatt
     9721 ttactttaaa taaaaattaa caaggtttat aatttgtagt agttaaagta tttaaagagg
     9781 tgggaacatg tgaaggttca aaggatagaa gtggagaata agccgtatcc attgtattta
     9841 ttactagata aagaatacca gctaatagaa ccagtaatga aatttattaa atacttagat
     9901 aatactggta agtctcctaa taccattaaa gcatactgct atcatttaaa gttgctgtac
     9961 gagttcatgg aacagagagg tgttattctt aatgatatta actttgagtt gttagcagac
    10021 ttcgtaggtt ggttgagata tccttcagca tcaaatgtaa ttgatcttca gtcaaaaaaa
    10081 gccataagag aagaaacgac agtgaataca attttaaatg tagttatgag ttttcttgat
    10141 tatttaagta gattaggaga atttaaatca attgatgtat ttaaacaagc aaagggaaga
    10201 aatttcaaag gatttttaca tcatgttaat aagggtagat accaaaagaa tgtcttaaag
    10261 ttaagggtta aaaagaaaca gataagaaca ttgagatcag aggaagttaa gcaaattatt
    10321 gatgcttgtc atacgaaaag agataaatta attttaatgc ttatgtatga gggtggttta
    10381 agaatcggtg aagtgttatc gcttaggctt gaagatattg tcacttggga caatcaaatc
    10441 catttaacac ctagagatgt taatgttaat gaagcttata ttaaattaag gaaggaaaga
    10501 acaatacatg tgagtaaaga acttatgtca ctttatacag attacttgat atatgagtat
    10561 agtgaggaat tggagcatga ttatgttttt atttccttaa aagaaggcta ttttgggaaa
    10621 ccactaaagt accaaagtgt tcttgatcta gttagaagaa tagttaaaag gactggaata
    10681 gaatttacat cacatatgct tcgccacact cacgcaacgc agctaattag ggaaggatgg
    10741 gatgttgcgt tcgttcaaaa gagattaggt cacgcacatg ttcagacaac gttaaatacc
    10801 tatgttcatc tttcagatca ggatatgaaa aatgagttta ataaatacct cgagagaaag
    10861 gagcataaga aatgaatgct tctagtaaaa ggaaaattat tagtcagagt gagattagca
    10921 aaaaaatagc tgtaatgaat gaagaaatgc aggggttttg ggctaataat agttgggata
    10981 taagaaaatg tccacatcct tctgccatag aattaagtaa gaatcctgct ttaaggaatc
    11041 gttgggttcg ttttgaacgt gttaaaaatc tgtggttaag aacagaattg aaatattttt
    11101 atttttacca tttaaacaat ggaatatgga atgcaaaaac tgtctggatt agaaaaggaa
    11161 cagtaattaa taaaatgtta gatttcttag atttaaagta tcctagcatt acttcaatta
    11221 ctgaagttcc tattgaaaaa gcaatgacgg agtatagaac ttatttgaca aaacggggtg
    11281 ttagaattac cactactaat tataagatta ctgctaatca agaaaaaaca cctgtaaaag
    11341 ctaattccta ctatgttact aatctaaaac aatttatgga gttttatgag aacttttatt
    11401 ttgatggaga ggagtgggat aaagacgttt gggatagacg taacttacct ttgccagatg
    11461 ataaggttaa cccaacacaa tatgaatata caattaactt taaagggttt cggaatacat
    11521 attttaaaca acttgtaaaa agatattgta agttgagatt gaacatggat agcttttcct
    11581 atgtaagtga tattgcccaa agacttaaag agttctttaa ttttctggac atgaaattta
    11641 aacaagttca gagagtacac caattaacga gagtggaaat tgaagcatat ttaagtgaac
    11701 taaacatgat gggaataaaa cctagtacaa taactgggag gatctctata ttggaaggac
    11761 tatttagtac ccttcttagg ctagaatggg atgatgttcc ttccaaaata ttaatttatt
    11821 ctgaggacta tccgaaaata ccaagagcaa aaccacgctt tatagatgaa ttcgtcctag
    11881 agcaattgaa cagtcatctt gataaattac ccgaatatat agctacgatg actatgattg
    11941 ttcaagaatg tggaatgagg ataagtgaat tgtgcacctt gaaaaaaggc tgtctattag
    12001 aggacaaaga tggagatttc tttttaaagt attatcaatg gaaaatgaaa aaggagcata
    12061 tagttccaat atctaaagag gtagctttac ttattaaagt tcgggaagat aaagtttcag
    12121 aggaatttcc agatagtgaa tacctctttc caagaaaaga tggatcgcca ttaaaacaag
    12181 aaacatttag aggtgagtta aataaattag cttatgagca aaatatagtg gataaatcag
    12241 gtgagattta tagattccat gcccatgcct ttcgccatac agtaggaaca agaatgatta
    12301 acaacgggat gccccagcat attgtgcaga aatttttggg gcatgaaagc ccagaaatga
    12361 caagcagata cgctcatatc tttgatgaaa ctctaaaaaa tgaatttact aaatttcagg
    12421 aaaaactggt taccaataat ggagatgtgc ttgatctaga tgaagataat gaagtcgatg
    12481 atgtagagct tcaatggttc aagaaaaata taaatgcaca agtgcttcca aatggttatt
    12541 gtagattgcc agtagtagca ggtggttgtc cacatgcgaa tgcatgctta gattgcactc
    12601 acttctgtac cagtaagcaa ttcttaccac agcacgaaga acagttagag cgtacagaag
    12661 agttattagc catagctaag gataaacaat ggcaaagaca agtagagact aatagccgtg
    12721 ttaaagagcg tttagaacaa atcattggaa gtttgacggg gtaattatca atggataaac
    12781 aagttagaaa tacaacagaa attgtacgtt tggcgaagca gaaatcaaaa aagacaaggg
    12841 aaaaagtaga caaagcgatt tctaaatttt cgattgaagg taaagttatt aattttaatt
    12901 caatagcaaa ggaagctaat gtttctaaat catggcttta taaggaacac gatattaggc
    12961 aaagaatcga atcccttcgt gagcgtcaaa taacagcaaa tgtagtctca aaacccaaga
    13021 aaagttctcg ttcggaggaa atccttatta aaaccttaaa aagaagagta atggaattag
    13081 aaaaagaaaa taaaaaatta cagaaccaaa ttcaaaaatt atatggagat ctgtataata
    13141 aagaataatt attaatctgt agacaaattg tgaaaggatg tacttaaacg ctaacggtca
    13201 gctttattga acagtcattt aggtatatgt ccaatctagg gtaagtaaat tgagtatcaa
    13261 tataaacttt atatgaacat aatcagttac gaggtgaaat catgagcaat ttgattaacg
    13321 gaaaaatacc aaatcaagcg attcaaacat taaaaatcgt aaaagattta tttggaagtt
    13381 caatagttgg agtatatcta tttggttcag cagtaaatgg tggtttacgc attaacagcg
    13441 atgtagatgt tctagtcgtc gtgaatcata gtttacctca attaactcga aaaaaactaa
    13501 cagaaagact aatgactata tcaggaaaga ttggaaatac ggattctgtt agaccacttg
    13561 aagttacggt tataaatagg agtgaagttg tcccttggca atatcctcca aaaagagaat
    13621 ttatatacgg tgagtggctc aggggtgaat ttgagaatgg acaaattcag gaaccaagct
    13681 atgatcctga tttggctatt gttttagcac aagcaagaaa gaatagtatt tctctatttg
    13741 gtcctgattc ttcaagtata cttgtctccg tacctttgac agatattcga agagcaatta
    13801 aggattcttt gccagaacta attgagggga taaaaggtga tgagcgtaat gtaattttaa
    13861 ccctagctcg aatgtggcaa acagtgacta ctggtgaaat tacctcgaaa gatgtcgctg
    13921 cagaatgggc tatacctctt ttacctaaag agcatgtaac tttactggat atagctagaa
    13981 aaggctatcg gggagagtgt gatgataagt gggaaggact atattcaaag gtgaaagcac
    14041 tcgttaagta tatgaaaaat tctatagaaa cttctctcaa ttaggctaat tttattgcaa
    14101 taacaggtgc ttacttttaa aactactgat ttattgataa atattgaaca atttttggga
    14161 agaataaagc gtcctcttgt gaaattagag aacgctttat tactttaatt tagtgaaaca
    14221 atttgtaact attgaaaata gaaagaaatt gttccttcga tagtttatta atattagtga
    14281 catttgcatg cttcaaagcc tgtcggaatt ggtttttagt gaaaagaaca cgatattcac
    14341 ggtttaccca cttataaaca aaagatcgat actttttgta gtccttcttt gaaatcaatg
    14401 gttgatgtcg ttcaagaaca atcaatacag agtctacact tggcttagga tgaaaatata
    14461 gtggtggtac ttttttgagc atttttatat ccatctccac cattaatagt aaacccaaag
    14521 ctcgttgcag attttgcaat cttttcgcaa atcccttctc aacgataaga tagctatatt
    14581 tagcctgact ttcaaaggta attcttttga caatatccgt actgatgtta taaggaatat
    14641 taccatatat cttatagttt atatgttttg ggaaggaaaa ttttagaata tccgtttgaa
    14701 tcacttttat attctcagag gggtttaccg cttctttagt cacttgacat aagcctccat
    14761 caatttctat agcagtaact gatcgactca ttttgactag ctctttggta aaatgtcctt
    14821 ttcctgatcc gatttctatt acgttgtctt gtttactgat attcgtgtga ttcaatattt
    14881 cttttacatg ctttttagaa gtaataaaat tttgcgtgtc tttagggttt ttctggttca
    14941 ttataacctt ctccttactg gttataatga actgacatta agcgttcatt ataaccaatt
    15001 aattttgatt tggttgataa cgaactttat taataacaaa tatagaaaaa gtacccatac
    15061 ttttatctcc tttccatttt tttcattatg aatgagataa agtaatttct actactgcga
    15121 tactggtgca cataattgaa acctccttat gtaactgaat atgattgtat catcttttca
    15181 ttttagaata aacctttata tgtaattttg cacttgacac ttcagatgga cgttttcttc
    15241 aaaataatat gttttgaaac ttgataagag aagccactgt ctttactaaa aagtaaaggc
    15301 tgcgataaag gacagtgact ttaataaagt tgcactgttg tattgtcttt ggataaaatt
    15361 gtctaaaggg aagttttatc cccaacgaaa aaacatctaa agtggtaagt aatgaaagaa
    15421 tgtcgataaa gtaggaatgg gggatctttt tgaatacaat agaatcacag tttgataagg
    15481 ttgcagaaga ttacgatttc gtgaatgagc ttttgaatga ttattcattc tttgtgtcta
    15541 atatgtctcc aaagaaaggc agagcattag atatcggatg tggctcgggt ttgttagtgg
    15601 agaaattagc aagttattat gatgaagtgg tagggattga tatttctaat caaatgctcg
    15661 atcttgccaa atctaaacgt caactaacaa atacggtcta tctgaatatg aatgcagaac
    15721 aacttaattt taatgagaag tttgatttta ttgtaagccg aacaaccttt catcatttgg
    15781 atgatatagc cagcgtaata cagcaaatga aggaactgtt gaatgaggaa ggaagaatag
    15841 ttattcttga taatgtatct gaagtcgaga caccgcctac ctatgtttat aaattagggg
    15901 caatccaaga gtttttaccg cattgtttca aatttggaat aaaaaatgcg attagaattt
    15961 ataatcacaa cacatctaaa tcatggcttg agcatttagc ttcagataag tacctatctg
    16021 aacaaaatta ttacgattta tatgaaaagt tgttgcctgg atgccaattc cataaaatgg
    16081 gttgggcaat gggggttgtc tggacaaaat aacttaaagc tgtggctttg aaaagttgaa
    16141 tttgtgattg ttttcagagc tatcgggtgc tttagtataa ataacaatga ataaaaaatc
    16201 agcctgtatc ttatgttact attaagtaat ttaagataca ggttttacta tttttgaaat
    16261 aagtaccttc cacttcaaat acatgtattc aaagtgaaag tattgattta aatgattctt
    16321 ttgtaattta ctcttgataa tatgaaattc catcgcagca ggatatgaat tttaccaaaa
    16381 ggatacaaaa gtgtggagaa atgatgggga tcactgtact ggaccacttg atcatcggac
    16441 gcaaacgata ttttagtttg agagaagaag gaatgatgga agagaagtaa gttgctttct
    16501 tgcaaaaaaa atgaagcaag tgtacaatta tattgtaatt ttgtttggga atagaagttg
    16561 aaacatctgt tgcacctcta tcgtacacga gcaatgttac aatttaaatt agtgcttttg
    16621 gcacgaggag gatattatta tggaaaacgg aacagtaaaa tggttcaacg ctgaaaaagg
    16681 atttggattt atctcacgtg aagatggaag cgacgtattc gttcacttct cagctatcca
    16741 aggtgacgga ttcaaaacat tagaagaagg tcaagcagtg actttcgacg ttgaagaatc
    16801 agaccgcggc ccacaagctg caaacgttgt aaaagcttaa ttttatctgt aaatcaaatt
    16861 gatggtagac atcaattttt acgagagaac aaagcgagta acaaaaatac ctgccacaag
    16921 agacaatttc tcttgtggca ggtatttttt tgtatgaaaa agtaaataaa tttaagaggc
    16981 tatgaaagag ccaccttgta tcaaggaata agaacaaaca aaaatggctt ctcatgtttt
    17041 tcatttgttt tctcttatat tcaggacaag gtgctgattt cataacctct tatttattct
    17101 tcatctggtg tcattaccat tggcaaaatc attggatgtc tttctgtacg ttcgaataag
    17161 aaaggctgaa gcgcgccagt catcgcttct ttcaatttat attctgaaca gtcaggtcca
    17221 ttcaaacatt ctcttaaagc gtggaataag attcgctgag cttcattgat catttctcct
    17281 gattctcgca tgtagacaaa tccacgagag agaatatctg gaccagccaa gatttctttt
    17341 ttcttgatat ccactgtcgc gacagctaat acaagacctt cttctgaaag caggtgacgg
    17401 tcacgtaaaa cgacattacc aatatcaccg atcccgctac catcgatata cacatctcca
    17461 gccacaaaat gcccagcagg acgcgcagaa tcgctagtta acgctaaaac atcgccattt
    17521 tccatgatga agcagttttc tgcaggaaca cctacatctt gtgctagaga agcatgtact
    17581 ttcaacatgc ggaattctcc gtggacagga ataaaatatt ttggcttcat caaacgaagc
    17641 attaattttt gttcttcttg tccaccgtga ccggaagtat ggatgttatt gatttttccg
    17701 tgaatcactt ctgcaccagc ttcagacagc aaattgatca aatgattgac acttgttgta
    17761 ttaccaggaa taggtgaact tgaaaagatc actgtatcgt ctggctggat ttggatttga
    17821 cggtgagtac cattcgcgat gcggctcaac gcggccatcg cttctccttg agaacctgta
    17881 cataggatca tcgtttcatt ggcaggcaag cgattgattt cgtgtgcatc tacaaatgtg
    17941 ccgtctggaa ctttgatata tcctaaacgt tgaccgttga cgatggcatt ttccatgctt
    18001 cgtccgaaaa cagcgatttt acgaccagtt gccacagcgg catctgctgc ttgctgcaaa
    18061 cggaagatgt tagaagcaaa gctggcaaaa atgatacgtc cttcgatttt tgaaaagatc
    18121 ttcaaaatag aatgcccaat cgttttttct gatttagtaa atgttggaat ctcagcattt
    18181 gtactatcag ataaaaggca caagactcct tcttccccga tacgagccat tttatgcaag
    18241 tttgctggtt cgccaacagg agtgaaatcg aatttgaagt ctccagtcgc tacgatattt
    18301 ccagaaggtg ttttcaccac gatccctaat gcatctggga tactatgggt cgttctgaaa
    18361 aagctgacag aagtctttct aaaacggatg atcgtatctt cattgatctc atggagctct
    18421 gcatctcgaa gcaagccatg ctcatctaat ttgtttgtga tcaacgctaa tgcgagagga
    18481 ccagcataga taggaatatt tgcttgtttc agcaagtagg gtattccgcc gatgtggtct
    18541 tcgtgcccgt gagtaatcac caaggctttt actttgtgta agttttgtat gatgtagctg
    18601 tagtcgggaa tgacatagtc aattccaagt aaatcgtctt ccgggaattt gatcccggca
    18661 tcaatcaaaa tgatttcatc ttggaattgt accccatacg tatttttccc gatttcacct
    18721 aatccaccaa tagcaaagac gcctgtctcg ttatttttga tggaaagttt catattaaaa
    18781 ctccactaat gagaaatcag cattttcttt ttcgtactct agatgacttc cttctagaag
    18841 ttgaacgaat tcaatattgt atggggtatt ttcttccaca cgttggcgag cgtctacatc
    18901 gctttttgcc tcaatgtaaa gagattttgt ttcttcccgt ttagggttgc gtacttttgt
    18961 ttcttgataa taaactttat agatcattgt tttgtctcct ttatctgcgt aaagcagtga
    19021 tttagctagt tgattttttt agacaacaca aaacgagggg acaagccacc tggtttttca
    19081 tatgctttga ctagaatact ggattcgagt caaagagaca agcctcgttt ttgcttatct
    19141 gttcggatat tttttcaacc gtaccttctg tataaaaaat atacaacaat taattgttat
    19201 tctagcataa aacagcaaat aagtatagaa atcaactaga ttgaaagaaa aaatcaatgt
    19261 ttatcatccc gctataatca taatgacttt tttatgagag aagtgttcta ttaataggct
    19321 cgttgtgcta tcatttttag taatgaaaat gtaacaaaag attaaaagga gatgagaaag
    19381 tgaaactaat gtggcattat accatgcgtt ataagaaatt cttgtttttc aatttcatat
    19441 gcgtatttgg ttttattttg attgaattag gacttcctac gattttagca cgtatgatcg
    19501 atgtaggaat ccgcaatgat gattttgatt atgtgaagca acaagggata tttatgatcg
    19561 ttattactgt aatcggtatt attttgaata tcttgttagg ttattttggt tcgaggatta
    19621 cgactaatat cgtagcagat attcgagatg atttgttcga aaagatccaa agttattcgc
    19681 atcaggaata cgaaaatcta ggcgtatctt cattgatcac tcgtacaaca aacgatgctt
    19741 atcagatcat gttgttcctg caaaatattt tacgtatcgg ttttatggca ccaatgatgt
    19801 tcattgtcag tttgtacatg gtcatgcgta caagtgtgac actcagtttg tatgtagtag
    19861 gtgcattacc tttactatta ttggcagtcg tagcgattgc taaggtgtct gaaccgttat
    19921 ctaaaaagca acaaaaaaac ttagataaaa tcaacagtat cttgcgggaa aatctttcag
    19981 gattgcgtgt gattcgtgca ttcgtcaatg aaaagtttga agaaaaacgt tttgctgtag
    20041 tcaatgagaa ttatgcttct agttcaaaaa gcttattccg tttgatggct gttgcacaac
    20101 cgggttttta tttcttattt aatatcgtaa tggtattgat catctggagc ggggcagtgc
    20161 aaatcgatgc aggcgagctt cgtgtaggag atttgattgc gtttatcgag tatatattcc
    20221 atgccttgtt ctcattcatg ttatttgcta gtgtgttcat gatgtatcca agagcagctg
    20281 tttcagctgg acgtatccaa gaagccctag atgccaaacc ttcgattgta gaagatccaa
    20341 atggtatatc tgatacaaaa actaaagggt atatcgaatt tcaaaatgta acatttgctt
    20401 atccaggcca ttcgcaagag ccagtgatac gaaatgtcag ttttacagcc tctccaggag
    20461 agaccgttgc ttttattgga agtaccggta gcggaaaatc tacgttgatc caattgatcc
    20521 ctcgctttta tgatgtgagt gaaggaaaga tcttgatcga tggagtagac gtgagagatt
    20581 atcggctaag caagttgcga gataagattg ggtttattcc gcaaaaagcg ctgctattta
    20641 ctggaacgat tgcagataat ttgcgatacg ggaaagaaga tgctacgcaa gaagaaatgg
    20701 aacgtgcagc tgagattgct caagcttcag aattcatttc tcgaaaacca gatggattta
    20761 atgaacattt atcagaaggt ggttccaatt tttcaggtgg acaaaaacaa cgattggcaa
    20821 tagccagagc catcatacgc cgtccggaaa tctatatttt tgatgacagc ttttctgcat
    20881 tagattatca aacggatgcg aagttacgtg ctcgtctgaa aaaagaaacg acagaatcta
    20941 ctgtattgat cgtagcacaa cgtgtgggaa cgatcatgca tgcagataag atcatcgtat
    21001 taaatgaagg agaagtcgtc ggtatgggga cgcatcggga gttgttggaa aaatgcccga
    21061 tttattatga tattgccgct tctcaattat caaaggagga attagcatga aaaaaacctt
    21121 ttcttcctta aaacgtttag ctcgatatat cagaccgtat cgtgagactt ttcttttagt
    21181 gatcctattt acagttctga cagtggcttt caacgtggct ttgccatatg ttacaggact
    21241 tcctactaca gaaatcagcc gtaatattgc caatggcgaa tcaatcaatt tttcttatat
    21301 attcaaatgt ctcatttgga tcacagttgt tggaataggg tattgtattt cgcaattact
    21361 ttctggtgtt ttgatgacaa atgtcgttca aagtgccatg catgatttga gaagagacat
    21421 agaagaaaaa atcaatcgtc ttccggtttc atatttcgat aaaaaccagc aaggaaatat
    21481 tttgtctcgt gtgacgaatg atgtagatgc agtcagcggt gctttacaac aagcttttat
    21541 tggtatcgta aatgcgatat tgggaattac gttggctgca gcaatgatgt tttatatcca
    21601 gccgatgatg gcgctgatct ctatgatcat gattccaaca tcgatctgga tctcaaaacg
    21661 agtgatcaat gcttcgcaga aatattttca aagcatgcag aattctcttg gtgagctaaa
    21721 cggctacgtc caagaaaata tgacaggttt tagtgtattg aaagtatatg gacgcgaaaa
    21781 agaaacattg gaaggattca aaaaagttaa tcacagcttg aaatactttg ggtttcgtgc
    21841 agcattcatt tctggcttga tgatgcctct agttcaatta actgcctatg ctacttatat
    21901 tggtatggct gttctaggaa gcttttatgt tattactggg gcgatcgttg tcggtcaact
    21961 acaagcattc atccaatata tttggcaaat cagtcagcca atggggaacg tgacacaatt
    22021 gtcttcagtt ttgcaaagtg catcagcggc aacgaaacgt gtattcgaga ttttagatga
    22081 gccagaagaa aaagaaaaca aagtagatgc ttcactgcca gaaacgattt taggagatgt
    22141 gacattcgat catgtcgatt ttgcatatga tccgaagaaa ccattaatcc aagatttgaa
    22201 ttttgaagtg aaagctggtc aaaccgtagc tgtggtcggt ccgaccggtg ccggcaagac
    22261 gacattgatc aatctattga tgcgttttta tgacgtagac aaaggagcaa taaggattga
    22321 tggaatcgac accaaatcaa tgagtcgcag tgatgtgcgt tcgctatttg gtatggtctt
    22381 gcaagatgca tggctttatc aaggaacgat tgcagataat atccgcttcg gaaagcttga
    22441 tgcgacagat tatgaagtag ttgatgctgc gaaaacagca aacgtagatc attttatccg
    22501 aacgatgcca gatggatatg atatggccat caattctgag ggagacaatg tgtctttagg
    22561 acaaaaacag ttgctgacga ttgcccgagc agtggtctcc gatcctaaaa tcttgatttt
    22621 agatgaagca acaagttcag tagacacccg tttagaagcg ttgattcaaa aagcaatgga
    22681 tcgcgtgatg gagggacgga caagtttcgt catcgctcac cgtttatcga caatccgtga
    22741 tgcagatctg attctagtga tggatcaagg tcagatcatt gaaaaaggaa cgcatgagag
    22801 tttattggca caaggtggat tttatgaaaa actgtataac agccaatttg ctgaggaagc
    22861 agaataagtc atataagaat aaaacagttt aaaacttatt ttttgttctc tattcttgat
    22921 aatggtaaag taaagttatc aagaaaagtg aggacacgga gatggaacag tttttaaatg
    22981 aaaaaattac tgaagccaaa agagaagttg atcgtttaac gcacgtctgt cagacgaagc
    23041 cttttgattc agctgatttt tttgaaaatg agtttcaaat cgaacgcttg gcagcacaaa
    23101 tagaagtgta cgaattaatt ttaaaaaaag taatagaaga aaatatagat gtacaataaa
    23161 aacaggtaaa acgtcagtta ccagatactg taaatagaca tctggcctga gcaggtttta
    23221 cctgtttttt ttcttatgaa ataaatatat gctatgatac ctttattcaa taggattagt
    23281 ttctacaaaa aatgaaagga gaaaaataaa tatggaacgg tttatagcat caaatgccac
    23341 agtgattggt gatgtgacat tgtcagaaga tgtgacgatc tggtatcaag ctgttttacg
    23401 cggggacagt aattggataa aaataggaca aagaacgaat atccaagatg gtacaatcat
    23461 ccatgtcgat catgatgcac ctgtagatat tgcggagaat gtgacggtag gacatcagtg
    23521 catgctacat ggctgtacaa tcgaaaaagg ggcgctgatc gggatgggga ccacgatatt
    23581 gaatcatgcg gttattggtg agaatagttt gattggtgct ggatcattag taacagaagg
    23641 aaaagttatt cctcctaatg tcttggcttt tggtcgccca gctaaagtga tccgtccttt
    23701 gacaaaagaa gaaatacaaa aaaacaaaga aaacatccag cattatgtcg agataggtcg
    23761 gatgcatgcg caaggaaaat tcaaggagca aagataggca gtagcatcca taaaataaaa
    23821 aaggatggtc agtttgagtt gaccatcctt ttttatttgg tttagataag agagcttaaa
    23881 aaagcttcct ttgaaacaga ttaatttaat gaagaactgt tttccatgac ttcatcgatc
    23941 aatccatatg cttttgcttg ttcagctgtc atatagttgt cacgatctgt atctttttca
    24001 atcacttcaa gcggttgtcc agtgcgttca gctaaaattt tgtttaagcg ttcgcgtgtt
    24061 tgcaaaatat ggcgtgccgc aatttcgatt tcagttgctt gcccttgagc gccgccaaga
    24121 ggttgatgga tcatgatctc agcatttggt aaagcaaatc gtttaccttt tgtaccagct
    24181 gtcagcaaga agcttcccat tgaagcagcc atacccataa caattgtttg gacatctgct
    24241 ttgacaaagt tcattgtgtc gtaaatagcc atacctgctg taactgaacc accaggagaa
    24301 ttgatataaa gatagatatc tttttcagaa tcttgagcgt ctaagaaaag caattgggcg
    24361 atgatcgaat tagcaagatc atctgttact tggccgctta acatgatgat acggtctttc
    24421 aataaacgtg agtagatatc gtaagctctt tcacctcgtg aagattgttc aatgactgta
    24481 ggaattaaat tcataataga gttcctcctt gatttcgttt agaaatagtg taccataatt
    24541 tagtttctaa tacctaaaat gtagtataca tttttggtca aaaaaggtca aataaaaaac
    24601 ttaggcaaat tagtcgcata aacccgcttt atggtatgat aagcagagaa acaaaacaag
    24661 gaggaacaac agattgatag ttggtattac tggccttatc ggagtgctga caggtgctgt
    24721 agttgtctat atcggcatga tactgcaaat gcgtcacttg gaaaataaag aacagcgtag
    24781 acgtgaattg gaattgaaag tcaaagaaat cgagacgctg aatcaattaa ataaaaagat
    24841 caacgagatc ttgcaaaaac ggacaacgct gatggaagaa tatgtcagct ttgatgcatt
    24901 tgatgattgt tatatcacga ttgacgattt tgcgtatctc caatcatttt cagcgcaaaa
    24961 tagtttttat ctgccaaatt atattttaga cgagctattt aagaatatct cccatcgaaa
    25021 agtgatttta tctccagatg aaacaatgaa gattggtgga tatgcttata aaggcggacg
    25081 cgtgatttta gagaatttta cggatagtct gactgaaatg atcaatgaaa aaaaagctca
    25141 gttgaagact atgacgcgcc agccgattgg tttcttctcg aaagatgatg tttggagctg
    25201 atattttgaa gcattgccgt tgcacatgag agcgtggtaa tgctttcttt ttttcttaat
    25261 gatacaatag aactgaacgg agtgaagaaa gatgttacaa atctatgccc atcgcggaag
    25321 cagcggcacc cacccagaaa atacgcttcc tgcatttgca gaagctgtac gagtaggtgc
    25381 agatggtatc gaactagatg tccatctaag caaagatggt tatttgatcg ttatgcacga
    25441 cgaagaagtg gaccggacga ctaacggtaa agggctgatt cgagaaaaaa cattggaaga
    25501 actcaaaaaa ttgaatgcag ggggcaaatt cagcaaagaa tttccagcag ctaaagttcc
    25561 tgaattgaga gaagtgttag gactgctcct ccaaaaaaat taccgaggga tgctaatgat
    25621 tgaaattaaa actgatcagt atgaatatcc cggtatcgaa gaaaaattag ccgaaacatt
    25681 aaaaagtaga gattggcctt ttcaacatgc atattgcagc tttcatttgg attctttgga
    25741 aaaaatcgca ttactggaac cggagataca gctggactta ttgatgagta cttctgataa
    25801 aaagatccaa ctagcagcag aaacccctta tatagaagga gtacatccaa aaaatacttg
    25861 gttgttcgaa catccagtcc aggcagctga ttttccaaaa gcaattcgtg catggacgat
    25921 taacgatgaa gaagaaatgg ccaaatgtca agaactcaaa atagatggaa taataactga
    25981 ttttccggag aaagctcttc ggtggaaaca atcaacgaaa agtaggtaaa atatggaaaa
    26041 agtattagtg atcgtcggtc cgacagcagt cggtaaaaca gcgctaagtg tggaactcgc
    26101 acaactattt ggaggagaga tcatcagtgg tgattcattg caaatctata aaaatctgga
    26161 tatcggtaca gcaaaaatca aagaagaaga gatgaataat gttccgcatc atttgattga
    26221 tgtgatcgaa cctactcaac gttactcagc cgctgatttt cagcattcag gtcgacaatt
    26281 gattaccgag attgcgaacc gagggcacct acctatcata gctggtggta cgggtctcta
    26341 tatccaatcc cttctatatg attatcagtt aggagcgaca gaagaagcga atcctgagat
    26401 tagaaaaaag tatgaaacgt tagctgaaac aattggttcc caagcattat ggaatcagtt
    26461 gaaagaaaaa gacccggcag ctgctgagaa aatccactgg aacaatcaac gcaaagtgat
    26521 aagagctctg gaagttttag agacgacagg acatagtatc acggcaccta aagaagagcc
    26581 aaagcaattg tatgattacc tcatgattgg attagatacg aagcgtagta ttctttatga
    26641 acgaatcaat caaagagtcg atttgatgct agcagaaggt ttagtagcag aagcgcgtaa
    26701 ggtgtatgag cttggagaag tccaggccag tcaaggtatt ggatataaag aatttttccc
    26761 ttattttgaa gggaaagaaa cattggaaga agcaacagaa caagtcaaac tacattcgag
    26821 aagatacgcg aaacgtcagt tgacgtggtt tcgtaaccgt ttggatgcac agtggtttga
    26881 tcttataagt gagccaaatc aaaaaagtca gattgagcaa acaatcaaaa aatggctgga
    26941 ggcggaataa aatggaagaa aaagtgatca tcgtaggggt agaaaccgaa gcaaaccagc
    27001 gttacttttc agagtcaatg gaagagctag ttcagctgac gaataccgca tcaggagaag
    27061 tcgtattcac aattacacaa aagagaccac aagtggatcg gcaaacgatt atcggtaaag
    27121 ggaaattaca agaattagtt caacaagcgg atgcgcatga agcggatttg atcatcttta
    27181 atcatgagct aactccgaga cagagccaac tgattaccga tgctgttggt cttccggtaa
    27241 ttgatcgcgt gcagcttatc ttagatattt ttgcgatgcg tgctcgttcg aaagaaggga
    27301 agctgcaggt tgaacttgct cagttagaat acctgctgcc tcgtttagta gggcaaggga
    27361 aaacattatc tcgtcttggc ggtgggatcg gaacaagagg gccaggtgaa acaaagttag
    27421 aaacagaccg ccgacatata cggaataaaa tttttgctgt taagaaagaa ttgaaagaag
    27481 tagaagctca tcgagagcgc aatcgccaaa aaagaaaaaa tagcgagatt tttcagattg
    27541 gacttattgg ctacacgaat gccgggaagt caactatctt gaatctattg acacaagcgg
    27601 atacttattc gaaagatcaa ttgtttgcaa cgttagatcc attgactaag agatggcgtt
    27661 ttgctgaagg atttgagatt actgtaacgg ataccgtcgg gttcatccaa gacctgccta
    27721 cgcaactgat cgatgctttc cattctacat tagaagaaag tcaaaatatg gatcttttgc
    27781 tacatgtagt cgatgctagt tctccagatc gtattttaca ggagcagact gtcttaaaat
    27841 tgatggatga actgaatatg aaagaaatgc cgatactgac tgtttacaat aaggcagacc
    27901 agatcgaccc agcgatgttc acgcctacgc tattcccaaa tgtattgatt tctactcaga
    27961 cgaaagaagg taagatggcg ttgatcgaag caatcaaacg tcagctgatg gaactaatga
    28021 ccccttatac actttctgtg ccaagcaacg aaggacagaa attaagcgag ttgagaagac
    28081 aaacaatggt tttggacgaa gcgtacatgg aagaaacaaa cgaatatgaa gttaaagggt
    28141 ttgcaaaaac caattcaaaa tggttaagaa aaaaccaata aaattttatt tttttaa
//
LOCUS       DAJSBN010000032        26439 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJSBN010000032 DAJSBN010000000
VERSION     DAJSBN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.032"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            143..2203
                     /locus_tag="ONT86_001632"
     CDS             143..2203
                     /locus_tag="ONT86_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HCR2352430.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2286..2855
                     /locus_tag="ONT86_001633"
     CDS             2286..2855
                     /locus_tag="ONT86_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2352431.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3030..3836)
                     /locus_tag="ONT86_001634"
     CDS             complement(3030..3836)
                     /locus_tag="ONT86_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352432.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3974..5488
                     /locus_tag="ONT86_001635"
     CDS             3974..5488
                     /locus_tag="ONT86_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCR2352433.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5891..6874)
                     /locus_tag="ONT86_001636"
     CDS             complement(5891..6874)
                     /locus_tag="ONT86_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCR2352434.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6879..7526)
                     /locus_tag="ONT86_001637"
     CDS             complement(6879..7526)
                     /locus_tag="ONT86_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2352435.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7611..8243)
                     /locus_tag="ONT86_001638"
     CDS             complement(7611..8243)
                     /locus_tag="ONT86_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2352436.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8259..8810)
                     /gene="coaC"
                     /locus_tag="ONT86_001639"
     CDS             complement(8259..8810)
                     /gene="coaC"
                     /locus_tag="ONT86_001639"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCR2352437.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8807..9544)
                     /locus_tag="ONT86_001640"
     CDS             complement(8807..9544)
                     /locus_tag="ONT86_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCR2352438.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9541..10530)
                     /locus_tag="ONT86_001641"
     CDS             complement(9541..10530)
                     /locus_tag="ONT86_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR2352439.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10788..11330
                     /locus_tag="ONT86_001642"
     CDS             10788..11330
                     /locus_tag="ONT86_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2352440.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11347..12432
                     /locus_tag="ONT86_001643"
     CDS             11347..12432
                     /locus_tag="ONT86_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2352441.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12444..13250
                     /locus_tag="ONT86_001644"
     CDS             12444..13250
                     /locus_tag="ONT86_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2352442.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13251..14078
                     /locus_tag="ONT86_001645"
     CDS             13251..14078
                     /locus_tag="ONT86_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2352443.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14075..15148
                     /locus_tag="ONT86_001646"
     CDS             14075..15148
                     /locus_tag="ONT86_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2352444.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15576..18272
                     /locus_tag="ONT86_001647"
     CDS             15576..18272
                     /locus_tag="ONT86_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2352445.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18349..18837
                     /locus_tag="ONT86_001648"
     CDS             18349..18837
                     /locus_tag="ONT86_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2352446.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18849..19343
                     /locus_tag="ONT86_001649"
     CDS             18849..19343
                     /locus_tag="ONT86_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2352447.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19364..20173
                     /locus_tag="ONT86_001650"
     CDS             19364..20173
                     /locus_tag="ONT86_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2352448.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20170..20991
                     /locus_tag="ONT86_001651"
     CDS             20170..20991
                     /locus_tag="ONT86_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2352449.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21042..23141
                     /locus_tag="ONT86_001652"
     CDS             21042..23141
                     /locus_tag="ONT86_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCR2352450.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23138..24256
                     /locus_tag="ONT86_001653"
     CDS             23138..24256
                     /locus_tag="ONT86_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCR2352451.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24295..25173
                     /locus_tag="ONT86_001654"
     CDS             24295..25173
                     /locus_tag="ONT86_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2352452.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25364..25516
                     /locus_tag="ONT86_001655"
     CDS             25364..25516
                     /locus_tag="ONT86_001655"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR2352453.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVLVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8375 a         4566 c         5563 g         7935 t
ORIGIN      
        1 tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact ctcctaatag tccatcttct
       61 tttgatttta tttatataat gaaatcatct tatgaaaatt actacttcat gtttagacaa
      121 gagtagggag gactaagaat aaatgtgttc aaaaagacgt tcaaaacaac taaataaaca
      181 cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa cactacgtta agtttatgat
      241 gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg catgtacccg ttcaggcaac
      301 gacacaaacc atcaaggtcg tacgtacctc tgtttctgag accactgatt cccctatcac
      361 tattactcat actttcactg atgcaaatgg aaaacagcta cttcctgcat tgacttccta
      421 taataaagtc agttatcctc caacgataaa ccactactgg ttaattccta acaaaacatt
      481 atggacatcg gaaaccggaa gcagtgtgcc gctttctatc ttttccgata tgactgatcc
      541 tacaaaaggt gatcagttaa aacagatcac tgatattttg aatcatgctt tgacagatat
      601 aaaggacggt ggaagcgcac aagtttctta tgtatatgaa aaagattatt cacagtttga
      661 aagaaagaaa atcgatgttc tggcagggac aaaagtttcg atatctgatc tggtagaaag
      721 gctgaagaac gcagatggaa cacaaggaga tatgtccaaa gtaatttcaa acaatgggaa
      781 tatcgtggat acgactaata caggaacatc tactatttcc ttgtcctatt ttgatccaat
      841 tgctttaaaa acaatgactg catctgctgt gatccatgtt cgtgctgatc aaacagctat
      901 caaaggaaaa gatcttcact taagaacagg agagagttac gcccctaaag atcttgttga
      961 atatgtaaca aaaagcgatg gaacttcagg aaataaagaa gaaatcaaaa tgaacgggac
     1021 agtagatact acaactccgg gagaatatcc cgttgtatta acttatatag actcgaaaac
     1081 atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa gcatctgctg cttcatcgac
     1141 tgtcatcact cattttatag atagtcaaac aaataaagaa cttcaagcag aaacacatat
     1201 gagtagtgta aatcgtattc ctgatttgcg cctacctgct gaattttctg atcgagtgcc
     1261 aaatatagat ttatcgactt atgaacaaaa tgggaaagtc acttctattt ctgattggat
     1321 gcaagcaaac ggattagaca gaaaaagtca ctggtcggag atccttgaag tacttcactc
     1381 agaactgatg aataatagga agcaatcagg aggggaaatt acctttactt atgtttatat
     1441 accagacaat cgttcattag aaggtcatga tcttattgtt catccaaacg aaaaaataaa
     1501 caaagaagag ttgatccaat cagcaacaaa ttccttaggt aaacccgtac cgttaaagga
     1561 tgtatcggtt acaatcaatg aagtaccatt ggaggatggt tatttgacca aacaaacagg
     1621 tgattttacg attatgtata gttatcatga cccgtattca ctccaagaac tccatgctca
     1681 gtcactatta catgttttac cattaaacaa acagatagat caaaaagaac aaaaaagacg
     1741 agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat gcagtttctc caacagggaa
     1801 agtaaaacaa acaagaagcc tagagttagc aagaatatct agaaaaatct cagaatctaa
     1861 taaaacgata aggcaagaag ctcatttggc cccacaagtt tctggattgg aaaatgaaat
     1921 tttagagcct aaaataaaac aacaatcaga aaaagaattt gttgctaaac agtcggaaga
     1981 tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa cgcttgtcaa ctattgattc
     2041 caccgatgag catctgcagc cgcaagtacc acttcctagt ccgggaacac ccgcacctgg
     2101 tggaggtatt ccttcttctg ctttaggaga tttcatgcga ggtatggcag gaacatgggt
     2161 atacgctaat cgaaatgaat ttgacgcaga tgactggttc tgatttatat ctctatctgt
     2221 tatagtcaaa taagtagctg agtcatatgt tatttggcat aatggagacg aggagggata
     2281 gaaaaatgtt agataaaacg ttaccttacg cagaaatctg gatgacaaga ccacttcaac
     2341 aaaatgttcc aatcacgcct ttagcggaag gttttgaatt tcgattttat caaaaagaag
     2401 atgaatctgc ttgggcggag attgagactt ccgttgggga atttcaaacg acagatgaag
     2461 cacttgagta ttttcaacaa aactttgctc cttatcctga agaacttgaa agaaggatgt
     2521 tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg taccgcttgg cgaaaaaaat
     2581 caagagagca agtgtatccg gtttttcatt ggttagcagt gaaaccagat taccaaggaa
     2641 aagggctcgc aaaagcattg actgccagag tcttgcaaga atttcctacc ctccatacgg
     2701 acggaccaat ctatcttcac acacagacat ggagccatca ggcgattgct ctttataaaa
     2761 aaatgggttt tacttttatt gctgaaaatc tagacggcac acctaatcca gattatgaaa
     2821 aagttatgca ggttttaagt aaattagata aataacgaaa tggtgaaata agtcctcagt
     2881 attgggataa aaaacgaaat agtcattcct catatcttac ttttttgagt taatactatc
     2941 ggataactaa tggcatgcca tacattcaaa aaagaagggg cagtgacaaa agtattgtca
     3001 ctgccccttc tcttgttttt tagcatgttt tatcgtttga tttttttagg aataaaattc
     3061 ttgatgaacg taaatttttg gtaatcgttt cgactataga ggtaaccaaa tacactgaaa
     3121 acaactgctc caataaaatt aacaaataaa tccttcatag tatccatgat tcccacgtct
     3181 aagtaaccat tttggatgat tgttttatct gttttaccat ctgcattttg actttcaatg
     3241 attgtccgag tgatatctgg aacatgatag acagtgtttc cgtctttact taactccaca
     3301 ctattgattt gctggatgat cgtatctttt tgcatatcga attgcagtaa tttatctgct
     3361 gaaaattcca caaactccca cataacacca actgtcatcg aaaaacagaa ggaaacaact
     3421 gctagtaata aaggagtcaa gttcatggtt tttgtatttt tattgagcaa ataaacaagg
     3481 gaaaagccga cacccccagc taaaaatccg ctgaatgcat gtagaaaagt atcccacatt
     3541 gaaaaatagc cataaaaatc acttaactct cctaaaactg ttgaactaaa aataaaagat
     3601 aacaaaatga tttctagcat atttggaatc tgtatcttta atatcttttc cagaaaataa
     3661 gggatttcaa ataaaattaa tgtcagtaat aaaagcattg tatttcccca gtgttggttc
     3721 cagacctgat ccactagcat aataacgacg atgatacgta ataaaatata gattccgttt
     3781 gtgattctac ctgcagtttt tgtactgttc gtaaaaaagg ttttcaaatg attcataaaa
     3841 taactccttc atgtatatcc ttaattatac taagaagcgt gtgtttacca aagctatatg
     3901 acttaaagaa aaacagagtt gatattgtga aaaaaatcac ttaatatagt ataaggagaa
     3961 aagggggata caaatgaaaa aattagttgc tggtttatta acagccgttg cacttcttgc
     4021 tatgagcgca tgcggcgcag atcagaaaaa tacagcgcag acaagttcta gtacgaagga
     4081 atctgccgaa gtatcatcag gcgcttctga acaagtatat acagatccaa aagaaatgaa
     4141 agaatcttat gatgtcgtga ttattggagc tggtggtgca gggatggcag cagctatcga
     4201 agcaaaagat gctgggttga atccagtaat tttggaaaag atgcctgttg ccggaggaaa
     4261 cacatctaaa tcatcaagcg gtatgaatgc ttcagaaaca aaatttcaaa aagaagaagg
     4321 aatcaaagac tcgaatgatg ccttctatga agaaacgcta aaaggtggtg gaggaacgaa
     4381 tgataaagaa atgcttcgat actttgtcga tcactctgct gatgcggtcg attggctaga
     4441 tcaaaacggt atcaaactgg acaacttaac gatcactgga gggatgagtg aaaagaggac
     4501 acatcgtccg tctgatggct cggctattgg tggatactta gtggaggggt tattgaataa
     4561 cgtccatgag cgggaaatcc ctatttttgt caacgcagat gtgacagaca ttaagaaaaa
     4621 tggggaaacg gtcaatgaag tgactgtaca tgttcaagga gaagaaccaa aagaggtcac
     4681 cggaaaagca atcgtagtta ccacaggcgg ttttggtgcc agcaaagaat ttattgaaaa
     4741 atatcgtccc gatttagctg attatattac gacaaatcaa gaaggatcaa ctggagatgg
     4801 tatcaccatg attgaaaaat taggtggaca aaccgttgat atggataaaa tacaaattca
     4861 tccaactgtg cagcaggatg agggggtctt gattggtgag gttgttcgcg gagaaggagc
     4921 aatcttggtc gatcaagagg ggaaaagatt tgtcaatgaa atggatacaa gggataaagt
     4981 atctgcagca attacagcat tgcctgaaaa atcagcttat ttgatttttg accaaggcgt
     5041 gcgtgatcgt gccacagcta ttgaatttta tgaccaaaaa ggatatgtca caactggaaa
     5101 gacaatggaa gaattagcag aaaaaatcga tttaccaaaa gaaacactag caaaaacagt
     5161 tgatggatgg aatacggatg ttgcggagaa aaaagatgct caattcaacc gtgccacagc
     5221 catggaacat gacttatcgt ctccgaatta ttatgcgatc aaaatcgcac caggaatcca
     5281 ttacacgatg ggcggtgtga aaatcaatac gaacactgaa gtattagata aagaaaacca
     5341 gccaattagg gggttatatg ctgctggtga agtcacaggt ggcttacatg gaaacaaccg
     5401 gatcggtgga aattcagttg cggagatcat tatttttggg cgacaagcgg gcatacaagc
     5461 agctcgattt gcttcggaaa atgaatgaaa tagaacgtaa ataaaaagag gttgtgaaat
     5521 cagcgttttg agctaatacc gagggtcagc tgattgaaca ctgtttagtg aagaggttgt
     5581 gaaaaagctg ccctgcatca agtaataaga acagccaagt aaaaacagct tcttatgttt
     5641 ttaacttggt tggacttatt accgcagatg caagcttttg aacaccgttt attcggaaaa
     5701 agagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
     5761 ggaagcagaa gaaattcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt
     5821 ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttgat
     5881 cagcatgatt ttattcagtt atcttcaaaa tgatttttcc gatgtttttg tttgcttcca
     5941 tatattcatg cgcttgtttg actgcgctga attggaatgt acggtcaatg attggccgaa
     6001 tcttgccatt ttctagaaaa ggcccagctt tttgcataaa agtttgtgcg agctctgctt
     6061 tatactgatc acttctaggt gtcaataatg taccaagcag ttggacacgt ttcccaatca
     6121 atacaccaag gtctatctca ggcacgatgg tcccacctag cataccaatc aagacccaac
     6181 gtccatcaac ggctatagaa gctaaattct tttcccagta agaagcgccg atgaaatcca
     6241 aaataacatc gactccccga ccttcagtga tttttcgtac ctcttcttga aagtcttgtt
     6301 ttttatagtt gatcaattca tctgcaccaa gttctttaca aaaggcgagt ttttcttctg
     6361 atcctgcagt aacgatgatt ttcgcgtgag aaagctgtct agctaattgg atcgctgctg
     6421 ttcccacacc acttgctcct gcatggatca agacagtttc gccttcttgt aattttccaa
     6481 gccagtaaag tgtttgataa gcagtcaaaa aaacttctga gattccagca gcagaaacat
     6541 agtctagcgt atcagggaga aggattgcac gatcggcagg catcacagca tattcggcat
     6601 aaccacctag attgactaat ccgtatactt ttgtcccagg agtaaaagtg gagatatcac
     6661 ttttattctc aactacctct ccagctactt cgactcccaa aatagggtag ggaggtttca
     6721 aagagactga ttctcttttc ataatatctg ttcgattgac agctgctgta tggactttga
     6781 tcagtaattc gccttgtttt ggtttaggaa caggcacttc aatttgttct aatacatcta
     6841 cagaaccagg atgcttgatt gaaatggcac gcatggatct attcctcctt tgcccattct
     6901 tggaatgctg tattcaactc tttgctacct tcagaaagta ctagagtcgt acgagaaata
     6961 tccgtttctt ccactagagc ggcaagtaca gcagcaactg tagagcgagt gatcgttttt
     7021 gatgccttct cattaggctt agttaattga atggaagtaa cttcttcatc gttagtcaat
     7081 gtgactggtt gaagaatgac aaaatctaag ttagttcgtt tcagccattc atctgcataa
     7141 tgtttagtaa tataataatc tgtcatggac tcaggccatt ttgctggttc atctgcataa
     7201 acagcactga ccatcagata gcgagagaca tgcgcatttt cagctgcgat cactgttttg
     7261 attgcgccat ccaagtctac ttgcagaaga ttctttcctt gagagccggc agcaaaaatc
     7321 aatacatcga tgcctttgaa tgcttcggtc attttttcca cggaccaagt taaatcaaaa
     7381 gagacatacg aaacattctc tttttttact aatgtttggg aagcaacatc tctcacccct
     7441 gcaaaaattt catgggaact tggagctaaa tcttttatca ggtgtcggcc aatttgtccg
     7501 tttgcaccta caacaaaaat tttcatttgg atcatccttt cgacgtcaaa tctttccatt
     7561 caatagtaac accaaaagag aaaaaaatca gagatcggct acgatgacta tcagttagta
     7621 agagaagggt gcttgaagta tcttaacaag acattgcata ctgctgcagt cgcaagacct
     7681 gcgacgattc cttcaggtat accacttgta aaaataaccg ctagaatagc tgaataaaca
     7741 gcatttaaat ttgttccgat gactttggca taatcttgct ggaataaaaa gtagatcata
     7801 ttcatcacta aaatcgtatt ggtcaatgaa ccggaaagtc cagctaagaa taaagaaacg
     7861 gaatctgctt tttgtttcat tagtttgcgc agacctttat aaacgaaata aggaaccacc
     7921 ccaatcaaga tgcgaggaat aaacgcgatc agcaaggctt tccagcttcc atgttctgtt
     7981 ccgatgactg ggataaacgg agaaaagaca aaagacaaag gtgtttggat aactgtgcta
     8041 cggatcatgc tgatcagtcc aaatgtaccg ccaagaaaag cacctagtct tggtcctaaa
     8101 acgatcgaag caatgattac aggaatatgc atggttgttg cattgattgg accgataggg
     8161 ataaaaccta aaggcgtaac agctaataaa atcaaaatag ctaaaaacat cgcagttaat
     8221 gtaaagtttt ttgtattttt cataagtact ccttttttct aagcatttct ttcattaaga
     8281 gtcagcatga cctgttcgat gatcgtctga atatcagcta aggcaccttt tccaaaatct
     8341 ccacaagcta aaagagattc tcttggttca atttcctgat agccgacaga tctcaatgtt
     8401 tccagattac gctgagtgat cgggttttga tacatattcg tgttcatggc aggggcaatc
     8461 aattttggtg ttcccactgg aagcgccatt gcgactgttg aaagaagatc gtctgcaatg
     8521 ccgtttgcta gcttgccgat aatgtttgct gtagcaggcg cgatgagaaa caagtcagct
     8581 tgttttgcta gttcgatatg attgattacc tgcggatttg attcttgcat gacgtctgta
     8641 tggactgcgc gcttcgacag agattgcaag gttaatggcg tgataaaatg tgtgctgctt
     8701 ttagacataa tcacatctac ctgggcacct aatttgacga attgacttgt aagatcagcg
     8761 cttttgtaag cagaaatact tccagtaacg cctagtaaga tctttttcat tttgattcct
     8821 ccagtctaat tttctcagta atcaatgcag caatctcaga tttactttga gcttcttcca
     8881 ctgttccatc tttagataag agatagccgt ggtgtgtctc gccatggatt tccgtcaaat
     8941 catttgcgaa aataaaatca gttttgttat ttatcagact attttttgcg actgccagaa
     9001 gagattcctt tggtacatct actaatagtt tgaaaccaat gatttttgct tttggctgcc
     9061 attcacgtag ataagaaatg atttttggtg ttttcttcaa aacgagcaca agatgctcgg
     9121 tgttagaaga gatctttgaa acagtggtat ctgtctgttc attttccgca aaccaattgt
     9181 ccagttcttc ttgcgttgaa gaagtgggta ggttttttgc cagctgttct tctgaaaaac
     9241 taaaagcagg agtaaaatca ctgactgcca tactgtggat aatcgcatcg tagtgttctt
     9301 ttttcgttag ttcttctaat tgtaaaaata agtccagtgc agattcgatc gtataaatcg
     9361 ttatattgga gtgtcttttc ggtttcagtg cttgtttcgt agtcacgtaa tcgattgtgg
     9421 tatccggatt ggcagctaaa taatcagcta atgcttgccc taatcgtcct gtcgaatgat
     9481 ttgtaatagc acgtacttga tcgatttttt cagtcgttcc tccagctgtg attagaatct
     9541 tcatttatgg tcacctacat ttactagtgt tcttccttgg tgtgttccag caagtagttt
     9601 agatgctgtt tcaggcaatt gatccagagt aatctcttgg acgaccagtt catctgcaat
     9661 ctgcaaatcc gccatctgtt gccataaaga aagacgttga tctattggta cattgacaga
     9721 atcaattcca atcaactgga tgtttcgcag aataaaaggc aagacagtcg cgtttagttt
     9781 gattccccca gcattgccac ataaagctac tgcaccgtta taagagatca atggaagaag
     9841 actgcttagc tgctcgccac ctactgtgtc gatgacatag tcgatttgct gttttcctaa
     9901 agcttttgtt gtctctggca gaaattcatc aggtcgtacg acttgcaaag cacctagact
     9961 ttttagccat gtgacagact cttcttttct ggaaaaggca atgattgact ggaatcctag
    10021 tttatgtaac agggctatcg ctgtactgcc tacaccgcca gaagcaccag taacgacaac
    10081 ttttttatcg gaggtcatac cttgacggat taaagcgttc acacacaaca tagcagtaaa
    10141 ccctgctgta ccaaggatca tcgcttgacg cagcgtcata ttttttggta gaggaactaa
    10201 ccagtctcct ggtacttttt gatattggct ataaccgcct ggatgggtga cgccaagccc
    10261 aaaaccagta gcaagtacaa gatcttcttt tgaaaaacgt ggattgttac tttggacgac
    10321 agttccggat aaatcaatcc ctggagtcat cggatatgtt cgtatcacct gaccagactc
    10381 gctcattgcc aatgcatctt tataattgac atcagaatag gcaattttta ccaagacttc
    10441 attttcagcc aactcagtaa atggcagatc actgattgct gtatggaaaa caggttcctt
    10501 tattacctga aaagatcgaa aagcgttcat tcgtgagaca ctccttttaa aattttcttt
    10561 tatcatacca taaagcctac tcttttattc ttgttcttcc gtaaatttag acaaaaatta
    10621 ggtgaaaaat aaagaatcaa acaactagaa tttattccaa aagataaaga aatattcagc
    10681 aaaaaaaatt ttacaaaggg ttgccttttt caataaattt cgtatactaa tacgagtggt
    10741 agtaaacagg aagtttacta ttagaaaact tcgattcgga ggaaattatg gaaattggtg
    10801 agaagttaag aaatttgcgc atccaaaaga atttgacaca agaagaacta ggcgaacgca
    10861 cagatttgtc aaaagggtat atttcccagt tggaacgaga tttaagttca ccatcaatgg
    10921 aaacattctt ttctattctt gaagtcttag gtgtgactcc agaagaattt tttcatcaag
    10981 aagcagcgaa tctgcaagtg gtttattcaa aagaagatca tacggtttat ttcgatgaag
    11041 aaaacgggta tgaattggaa tggctcgtgg ctgattcgaa tgaaaaagaa atggagcctg
    11101 tcttgctgac ttttgaaggt gcaggagaat ataagacgtt tgaaccttca ttatctgaaa
    11161 cgtttatttt tgtattagaa ggggaattag agttaagctt aggagagacg gtctatgtgg
    11221 caaaagcagg acagtcgatc tattatcaag cgacaaaaca acatcaatta aggaatcatc
    11281 tcaaaaaacg aacaaaagta ttgattgttg caacgcaatc atatttgtaa tatagggggt
    11341 aaaaacgtgg ggaaaccaat catttcattt gatcaagtag tgaaacgcta tgatgatgaa
    11401 acagtattaa aaaaagtaag ttttgagatc gaacaaggaa aattttatac actgcttggt
    11461 ccttccggct gtggtaaaac aacgatccta cgtattatag ctggattcac cgaagctaca
    11521 gaaggggaca tctattttga agggaaacgg ttgaatgatt tgccagctaa taagcgacaa
    11581 gtaaatacag tcttccaaga ttatgcactt tttcctcata tgaatgtttt tgataacgtt
    11641 gcttttggat tgaagatcaa gaaaatgtcc aaatcagata tcgaaaagaa ggtaaaagat
    11701 gctttgcgta tggtccagct tcctggatac gaaaaacggg agattagtga aatgtctggt
    11761 ggtcaaagac agcgtgtagc gattgcacga gcaattgtaa atgaaccgaa agtattgcta
    11821 ttagatgaac cgttatcggc gcttgatttg aaactacgta cagatatgca gtatgaactg
    11881 cgggatctgc aacaacgatt aggaatcacg tttatttttg ttactcatga ccaagaagaa
    11941 gcgttggcaa tgagtgatga gatttttgtc atgaataaag ggaaaatcgt ccaaagtggt
    12001 acacccgtag atatttatga tgagccaatc aatcatttcg ttgctgattt tgttggggag
    12061 agcaatatcg tggacggtgt aatgatcgaa gataatctag ttgagtttgt tggtaagcaa
    12121 tttgaatgtg tcgatggcgg gatgcgtcta aatgaaccag ttgaagttgt tttacgccca
    12181 gaggatttga cgatcacgac accagataaa ggaaaactag tcgtaacggt cgatactcag
    12241 ctgtttcgtg gtgtccatta tgaaatcatc tgttatgatg aacagcaaaa tgaatggatg
    12301 gtccattcaa cgaaaaaagc taaagaagga agcaaagtag gattggcctt tgagccagaa
    12361 gatatccatg tcatgcgttt caacgagtct gaagaagaat tcgatgcacg tcttgacagc
    12421 tacgaagaat aggggggaca agaatgaaaa caatgcgcag gatctattcc gtcccttata
    12481 ttttgtggct cggtttattc gtgatcgcgc ctgtattaat gatcatttat caatcttttt
    12541 ttgatatgaa tgggcaattc acgctgaata attatctatc ttattttacg tcggggacat
    12601 cgctttctat gacgatcaat tctgtatggt atgcattttt gatcacgttt ttcacgctga
    12661 tcatcagtta cccgactgct tattttttaa caaaactgaa gcacaagcaa ttatggctga
    12721 tgctgattat tttgcctaca tgggtgaatc tgctgcttaa agcttacgca tttattggga
    12781 ttttcagtgt gcacggcagc gtgaatcatt tctttgattt tatcgggatc gggccgaaac
    12841 agatattatt cacagacttc agttttctgt tcgtggcaac gtatatcgag attccattta
    12901 tgattatgcc aattttcaat gcattggaag agatgaatcc ttctttgatc agtgctagta
    12961 gagatttagg cgcgaatagc tttgagacct tccgacgagt gatttttccg ttatcattaa
    13021 atggtgtgaa aagtggtgtc caagcagtat ttattccttc tctttcttta ttcatgctga
    13081 ctcgtctgat tggcggaaat cgggtgatca ctttaggaac ggctatcgaa cagcatttct
    13141 tggttacaca aaactggggg atgggttcta caatcggtgt gattttaatc attgctatgt
    13201 tcatcgtgat gctcctgaca ggcgagaaga aaaaaggagg tcgctcataa atgaaaaaat
    13261 ttaaatggtc gtatctttat ttagggatcg tctttttatt cttatatgca ccgatcttct
    13321 atctgatttt ttattccttc aatgaaggag gaacgatgaa cagctttaca ggatttactt
    13381 gggaaaatta tgaagctgtt tttgaagata cacgtttgat cattatcgta ttgaatacgt
    13441 tcatgctggc tttcttatct gcattgatcg ccacgatcat cgggacattt ggggctatgg
    13501 gaatctatta cacgaaaaaa cgacgtgcac ggacagcgtt gttgagcatg aataacatct
    13561 tattagtctc tcctgatgtt attatcggtg ctagtttctt gattttcttt acactactag
    13621 gattcggatt aggttttcag agtgtattgc tttcccatat tgctttcagc attccaatcg
    13681 tcgtcttgat ggtcttgcca aaattgcagg aaatgaacga ttcaatggtc gatgcggcta
    13741 gagaccttgg tgctaataat ttccaagtag tcaaaaatat cattttgcct tatctttctc
    13801 caggaatcat agctggatat tttatggcat ttacctattc tcttgatgat tttgcggtta
    13861 catttttcgt cacaggaaat ggctttacga cgctctcagt agagatctat tctcgtgcaa
    13921 gacaaggaat cagtttagaa atcaatgcgc taagtgcgtt agttttcttg ttctccatga
    13981 tattggtcat tggatactac tttattagta aagaaaaccg tccaaaacgg ctgaagaaaa
    14041 acagaagagt acgcgagggg gtggcacggc tgcgatgaaa aaattacaat ctctgttttt
    14101 aggtattctc atcattattg cggtgctgct gcttggtgtt cgtcaacttg aaaaaacaag
    14161 tggcatgagt ggagcaaagg tacttaccat ctataactgg ggagattata tcgatcctag
    14221 tttgatcacg aaatttgaaa aagaatatgg atacaaagtg aattatgaaa catttgattc
    14281 taatgaagcg atgttcacaa aaatccagca aggtgggaca aactacgaca tcgctatacc
    14341 aagtgaatat atgatccaaa aaatgatcaa agaaaaacta gtacttccat tagatcattc
    14401 gaagatcaaa ggattaaata atattgatcc acgattttta gacttggatt ttgatccaga
    14461 taatcagtat tcgattccat atttttgggg aactttaggg atcgtatata acgataaatt
    14521 ctttgatgca aatcaaatta agcattggga tgatttgtgg aagccggaat taaaagacag
    14581 tttgatgctg attgacggtg cacgagaagt tatggggctt tctttgaata gtttaggcta
    14641 ttctttgaat agtaagaata tggcacagtt gacagaagca gcaaataaat tgaatcattt
    14701 aacaccaaat gtcaaagcaa tcgtggcaga tgagatcaaa atgtatatga tcaatgaaga
    14761 agcctccgtg gcagtgacat tttctggcga agctgccgat atgatgtggg aaaatgaaca
    14821 tttacattat gtgatcccac cagaaggatc caatctttgg tttgataata tcgttatgcc
    14881 aaaaaccgtg aaaaataaag aaggtgccta tgatttcatc aactttatgc tagaaccgga
    14941 aaatgcggca caaaatgcag agtatattgg ttattctaca ccaaatgaag aagcaaaagc
    15001 gttattgcca aaagacattt cgtcggacga acaattctat ccatctgatg acacgatctc
    15061 ccatcttgaa gtctacaaag atctaggatc gaaataccta ggcatctata atgacctatt
    15121 cttagaattc aagatgtata ggagataatg gcaaaactca gtaagaatgg ggacttatca
    15181 acgttctaaa gagctaaaaa tcgtcctttt tctatctgag ttttccaatc ttctaccaaa
    15241 agcggatgaa acgccgcttt ttttgtgtta ttttattttt tctaccacgt ttttctacca
    15301 gagaaaagtg gacaaaacca ttgtaaaata gggcccgaga cgggctagta aatctaagat
    15361 tgaaaaagtt acagaaggat aaagtaattg gaaactatgc aaatatactt aacccttctg
    15421 taatctatta ataaaactaa tatatgaatc cagttcaata tttgtagcta tgaaaaaaca
    15481 taaaaatcat attagataaa aactaaattg gtagataaat tattttgtca gatatctgag
    15541 gcaaatattc gactatttgt gtttcaaagt aaaaattgga atacaaatat agaatagaca
    15601 tattgcagcc gatagtttat tatgctttta gacacattaa actggaggaa aaaatgaaaa
    15661 agagcgctaa agatcgagtt tttgatgagc tgatacgact aacagggaat atgtcttctg
    15721 tcactgccga gaaaatttcg aagaatctag gtatctctcg tcagaatgcc agccattatt
    15781 tgacgagatt agtggaagat aaaaaggtcg agaaattacc gggaaagcct gttttgtgga
    15841 agcctttaga tgaatatgcg gttgtagata atacggagca aataaacgaa gcctttcatt
    15901 ctgtagttgg tcatgacgga agtctgagag aggtcattca aaaatgtata gccgctgtca
    15961 aatatccgcc taacggactc agtgtattaa taaatggagc cacgggtgtt ggaaaaagtt
    16021 ttttagccac aaaaattttt gaatacgcta tccatgagca aattattgaa aaagatgcac
    16081 catttgctat cttaaactgt gccgattatg cggacaatcc cgaactgctt tcggcaacac
    16141 tgtttggata caagaagggg gcctttacgg gggctgaaaa ggataccgat ggtttattgg
    16201 caacggccaa caacggctat ctttttctcg atgaggtcca tcgcctatcg aaagaaaatc
    16261 aagaaaagct ctttctattt attgatactg gaaactatcg tccagtcggt gaaaatgtaa
    16321 attggcattc tgctaaggtt cgttttattt tcgccacgac ggaaaaaggt gaaaattatt
    16381 tattagacac ctttgatcgg cgtattcaaa ttagtgttat gttgccaact tttgaagatc
    16441 gccccattcg agaacgatta gagctgattc aattattttt tcaaaatgaa gcggatgttt
    16501 tacaaaaaga tattattgtt tcaagagaag ccttgtcttt aatgttatct catcccttta
    16561 gtgggaatat tggtaagctg aagaatatca ttaaaatcag ttgtgcggat gtgtatagtc
    16621 gaacacaaga agaaccatta acgattggga aaaatgagat cttgattcag ttaaatatgc
    16681 tggaatccga agtagaaagc ttgccgattg ctagtttatt gataaagaaa aatcaaaaga
    16741 taaaaacgga tcgaatcaaa ccgtttgatt atgattcgat tttggaaaaa attactcagc
    16801 tgataaatga aggtaatttc aatgaaaatt tttcttcgat aaagaatgaa gtacagcatt
    16861 tggtgcatca cattgataag gattcttttt tatcaatgag tagaaaattg atgattcaga
    16921 tgtttagaaa ggtctgggta tccgttttag gaaaaacgta cggattgacg acgagtggac
    16981 ctgttgcaga aattattagc agatcctatg tcatttcccc aagctataag acagacttaa
    17041 ccgggataaa tcgtacatta gctgcaaatc tacctcggac gacttactta tgtaatcagt
    17101 tcttggagca tttaccaccg ctctcagaaa aggacgagaa ctttctaaga ctgcttattg
    17161 tcgtgacttt atctgattat gtagatgaat ccatcgagct gaagggcctg ttgcttgctc
    17221 acggagatag tacggctagc agcattcaag cagtagtgaa tcaattatgc ggcaattatg
    17281 tttttgaagc gttggatatg ccagtagaga gtaatgtgtc agagattgtc gaatcgacaa
    17341 ggaatttcgt gaaacggcaa gtgaatacag atagtctagt gctgatcgta gatatgggct
    17401 ctttaaatca aatttacaca cagatcaaga acgatttaaa aggggagtta cttcttctga
    17461 ataatttgac gacttccatt gcacttgata ttggcttgaa gatgacgatg aatgcgccat
    17521 ttaaacagat tgcggaggat gcagcagaca agtattcaat caatgttcaa tactttgaag
    17581 gcttttctca aagtagcaac attatcattt cctgtatgtc gggtttgggg atttcggaca
    17641 aattgaaaga tgtttttcgt catcatttat cggatgaatc catcgaagtt ttcacgaagg
    17701 actacagaga actacgtgaa ttaatcgacc aaaatgatga aacttatttt gacaagacaa
    17761 aattggtcat cacgacttcg gatcttccac gttccttttc aattcctaat attaatgttt
    17821 atgacatctt agacggtgat ggagcagcca atttggagca tattttgagt cctgaaatga
    17881 agccggcaga atttaaccgc ttgattcagg agctagtaaa attcttttcg ttggaaggaa
    17941 ttgcggaccg tttgaacttt ctgaacccaa cgatcgtgat gagtgaagtg gaaacagtca
    18001 tttctaaata tgaaaattac tatcatttca ctttaaatgg ccgtgtaaaa ctaaatctct
    18061 atatgcatac ggcattgatg atggaacgtt tattcttggc tcggacagaa aaatctgaag
    18121 aaccgaatta tgatttgaaa ccagatgaag atgaatttta ttatgtttct agaagtattt
    18181 ttcaatcaat ggagatgaaa tataatttta aagtcaatgc ttatgagctt tcgctgctct
    18241 atgaattatt aggaccgtat attcaaaaat aaagtgtcta aaaaagtgtc taatctgttt
    18301 tttttaggtt agacactttt tttaatgtga attaacgtga ttaaaccaat gtttttaaaa
    18361 gttggcacag gaattgctta tataaaagtg aacagaaaga aaggaattaa ccatatgaag
    18421 aaaattctaa tagcgactca cggtcactta gcttcaggct ttcttagcag tattcaatta
    18481 ctgacgggga aggttcaaga gattacggtg attaatgctt atgtggatga gtgtgatttt
    18541 gaacaggagt tgaatgtatt tgtaaaccag gtgaacgagc aggatcagat ttttgttttt
    18601 accgatttat ttggcggaag tgtcaatcag aagattacca aaaccttttt ggagaaagag
    18661 attgctgtaa ctattttgtc tggctttaat ctgccaattc tgctcgaaat agtattggca
    18721 accaaggaat taacaaagga ggatattcag gaactggtaa ataaatgcca acaggaatta
    18781 aagatgacca ttcttgatga ggtacatgta gtaaatgaag aggaagattt tttttaggag
    18841 gaagaaaaat gattaaagca gtgcgaattg atgaacgatt aattcatgga caagtagcga
    18901 tgacttggac aaagagtcta aatttaaccg ggttagtagt agcaagcgat gaggcggcta
    18961 ccaacgatat tcaaaagatg acattgaaga tggctgcacc atcgaatgtc aaagtcatta
    19021 ttaaaacagt tgatgacgcg attcatttgt taaaagatcc gcgggcgaag gacatgcgct
    19081 tgatggtttt agtccctaat gtcaaagatg cggtaaaagt tgcgaaagct ttcccaacgg
    19141 aaatcgaatt aatgaacgtg gggaatgctg ggaaaatgac accgccatcg gaagataaga
    19201 aaaccttatc aaaggaagtg atgttaacga cgtcagaaat tcaagactta aaagaattag
    19261 ttgaacttta tccagatacc gtttttcagc ctacaccggc gatggaaaaa cgttcagcta
    19321 tctctatttt gagtgatttt tagaaaaagg aaggagaaaa attatgtatg atgcatttgt
    19381 agtcgctttg gccgttttta ttggaattgc tggacatgaa atgttcggga tgtccatgct
    19441 gagtcgtccg attgttgttg ctccgttagc aggagcgttg atgggggatt tgcagacggg
    19501 gttgattgtc ggggcgtcac tagaagccat ctttatgggt gtcgtcaata tcggaatctc
    19561 tagtacggcg gagccttctt tggctgctgg gctggctaca gcgttcgcaa tcaaaatggg
    19621 aggaaacgtg gatgcgatta ttcccattgt ctttccacta gcagtattag gattacagtt
    19681 aatgaatatg attttttctt ttgtatgtgg accgatggct gctaaatttt tcaaatttgc
    19741 aaaatctggt gatcaaaaag gaatcatcaa tctgcacttc tttatgtggt ttgtccacta
    19801 tgcgctttat gctcttattc cattctttgc cgttttattt ggagcagatg ttgtcaaaag
    19861 cgtcttggat aatattccag aggtcatcat gaacggatta acagtcgctg gaaatctgct
    19921 gccggcagtt gggatggcga tgctgttacg gatgctttgg agtaaaaata ttgctgtgtg
    19981 gtttttcttt ggggccgttg ttatggcgta tctcgagtta ccattgattg caattgccgc
    20041 agttggaacg attattgcaa ttgttatagc ccaaagagat ctacaactaa aaaatatatc
    20101 tgttcatgca gttcaaagcg atgctgctgt aaatgcaacg cttgaccaag aagaggagga
    20161 tttctttcaa tgaaattagc tgatggaatt tctaaagatg agaaaaaatt ggttcggaag
    20221 atgttttggc gctcgatgac tatgtatgct tcggtgaatc cggtgacgat gggcggcggt
    20281 ggtttcgcct attctatgat gcccttcatt aatcatttct acaaaagtga agaagatcga
    20341 gctgcggcgc tggaacgtca tacacagtat ttttcgacga ccattccttg cgcgtccttt
    20401 gtcatgggga ttgcggcatc gatggagaag gaaaattctg aaaaagctgg ttttgacgtg
    20461 gaaagtatca acgcgatcaa aacgagtttg atgggaccat tggctggaat tggcgattca
    20521 ttgttctggg gagtttggcg tgtcgttacc gcgggactag cgatcaactt agccaatgcc
    20581 ggaaatattt tggcaccaat tatcttttta gttatgttca atatacctaa ttacttgatg
    20641 cgatactatg gcggcgtttt aggctattca ttaggctcta aatatattga aaagctgtat
    20701 gcgaacgggt taattgaaat tctgacaaaa gctgctagta tcttgggtct gatcatggta
    20761 ggtgcaatga cgatcagtat ggtcaaattc aataccgttt tgagcttttc aatgaagggc
    20821 gaagaactaa tgaatgtcca agaaatcctc gatagtattt ttaaaggaat tgtaccgtta
    20881 ttggtaacat tgagctgttt caaattacta gataaaaaag tcggcgtcat caaaattatt
    20941 tttggaatgg tcgtcttggg aattttattg tcattacttg gaatcgcata gtaggaagct
    21001 gcagctacta ggctgcagtt tttttagcaa ggagggaaaa gatgactatt aatgaagtaa
    21061 tttattcacc caaacaccca ttttttaatg ctgagttaaa cgacggaaat gtgactactt
    21121 caaacgaata cagctactat cggaatattc ctgatgtaaa gggattgacc ttcgatgtaa
    21181 gtaatcaaaa gacattagca aaccttgatg tttgcgggac gattcaaacg ttgacctttt
    21241 atcgagaaaa tttactaact gaggaaaaac caggtgtttg ggtcaataaa cagctctctc
    21301 aaaccaattc gttatcctta aaggttgaag taaacgggat tgtctaccat ttgtcagaat
    21361 cggatcatcg tattgaagtc gatgtgattc aagactgttt gcctagatat attcatcatt
    21421 attcgacgtt taaggtcatt gtagtatcct ttgctcccat actggctaat aagcgtttaa
    21481 gtatgcttgt tcaacaggtt tacattgtga atgaaacaca cgaaagcctt caagtttcag
    21541 tgaaagatgt gccttcgtat caagagaagt attcggatca gcaaaatgtg ttgatttctc
    21601 aaaaaggaaa taaggacacg atcgcacaac aggaagtggc aagcttcgcc attgcttttg
    21661 tggacccaaa tgcttttgaa gaagtaatga cttttcagga atctgatatc gaaaaatggc
    21721 tgaaggaaac attgaactat tttgctcagc tatttgggca gctgtcaatg ccaaacgata
    21781 gagttgtcca tctctatcgt cgtgcacttt atcaaagctt ttctagtttc ggtatgaatc
    21841 gacagggaca ggtcgtcggc agcaattggg gcagctatcc tgctactaat cgaatttgga
    21901 ataaggatat gtattacgca tcattgccat tcaccatgtt tgaaccggag ttatgtcaaa
    21961 aaacggttct atggtttgat caatatggcg ttaagtttcc tggttcaaaa tttacaggag
    22021 gtatcaatca ttctctgagt aattcgttat ccagtgcgtt actggctagt ctttattttg
    22081 aacatactgg ggatacaaaa ttctttgagc ggtgtccgca ggttctactg aatgcttcaa
    22141 gaattattga ggacatatta gctcaacgtc ggtcagggga accaatgctt tttccatctg
    22201 tttggttatc agacgccttt gcacttggaa agtatcatac cggcagcaat ctttgtcttt
    22261 ggaaggcctg tattggactt gcagaaattt ttgaagccct agaacaactg agtcttagca
    22321 agcgatacaa aaacattgcc tgtaaactaa aggaatcaat ttgtaaacaa atgactatcg
    22381 atggtccttt tggtgagcaa tttttggagg gaatcggtga tgaggagaaa acaacctatt
    22441 cagtgaagca ctatcaaaaa ccgattcttg agcaagggtt aatcttttta agtgatgtga
    22501 ttgtggatgg aaaaattaat ttattgatgc atgatggaga agaatcagac actacgttga
    22561 tgccttttta tcaattttta gataataaag atcatttata tcagaacacc atggcctttt
    22621 ctgctagttc tttcaatcca acctatagcg aggaaatcaa aggcatcact tggggattag
    22681 aatccggcgc cacttttcct ggctttatca ctgttttaat gagcgagctg cacaaccctc
    22741 aagctttcgt aacccgatta gaagaactaa tttgtttggc agatttagac ggttcgtggt
    22801 ggtggtggcc gtatcgctta gaggctcaac aaggagaagt cgtgcgagac tttggctgcg
    22861 gcaaatgcgg ctgggcatcg ggtctttttg tcagcctaat gatcagtcaa tatttcggat
    22921 tgaaattcaa aaaaggagta ttgcaaattg atcctgttga taatttaacg ttttcttgga
    22981 agaatttgaa gttcggtcaa gccttcattt cgattgaatg tacgcaatcc gagttgagta
    23041 tttcaaattt gtcagaaaag ccattgaaaa taagtgatac gaaaaaaatt cttcacgtag
    23101 aaaagggaaa aacgattcac attcaaagga gaaaaagatg agtatactaa aaaatattac
    23161 ccaatttaaa aaaatccaga gtcgagcaat cactcccgaa aatccaacag gcgagaaagg
    23221 taaagctgcg atgttgccaa gcgagctagg accaagccgc aaaggtcgtg gcgcaattcc
    23281 gctgacttct ggtgaagaaa ctgtgattgc ggatattact ggaacgggaa taattcgtca
    23341 tatgtggatg acgattcgtg atcgaacaga atttggcagt tttgttttac gtgatttaat
    23401 cttaagaatt tattgggatg ggtcggatag ccctgctgtt gaaagtcctt taggggattt
    23461 cttttgtaat gggtttggag agcgttgcga ggtgaactcg ttgccgatag tggttaatcc
    23521 tacagggggc tttaattcct atttcgaaat gcctttccaa acatccgcta aaattacgat
    23581 cgagaattta catccgaagg atatgtcctc atttttttat acgataaatt acgctttggt
    23641 ggacgaattg gaagaaaata gcctatattt tcacgcaaat tggcatcggg aacgcattac
    23701 caaattacag gaggattata agttaattga tcgattaaag ggtcaaggct attacgtggg
    23761 tacttacctt gcgcttacct gcctagaacg ttattggtgg ggcgaaggcg agtttaaatt
    23821 ctacattgat ggggatagtg agtatccgac cgtaacctca acaggttcag aagattattt
    23881 tggcggggcg tgggcctttc atgagtacga tgagttcgga cgtccacatg cgaaaaacta
    23941 ttcaacagcc tttttagggt atccttacca atcaaataga gatgccacaa gagatcgttt
    24001 tgaaacggga aaattgaatg ccgttcatgc ctttggtgac gacgcaatgc cacgacatgg
    24061 tctttatcgt tggcatcttc aagaccccat ctgttttacc gaagatttat cggtcacttt
    24121 acaacaaatc ggcaacgatg atgttcgctt atttgagcgg caagacgatg ttgctagtgt
    24181 cgcttactgg tattcaacag aagcttcagg aaatactaaa cgtcatttca ctgatcgaga
    24241 acgcttgccg cgttaatgaa gtgtttttca catattcgaa gggaggaacc tatgatgata
    24301 tatggggcaa ttgaagctgg agggactaaa tttgtctgtg cagtcggtac tgaggaatta
    24361 acgattttgg aaagagtcag ttttcccaca acaactccag ttgaaacaat gaagcaggtc
    24421 attgattttt ttaaaccttt taaggaacaa ttggcaggca tcggggtcgg ctgctttggt
    24481 ccaattgata ttcaatgcaa atcaacgagt ttcggtcata tcacatcaac accaaaactt
    24541 gcttggcaaa atttcaattt tgtaggaacg ctaaagcaat atttcaatat tccgattgaa
    24601 tggacaacag atgtcaatgc ggcttgctat ggtgaatatg ttgctggttc tggcaaaggg
    24661 ttagcaggtg ttgcatactt cactgtggga acaggtatcg gaggtggtgc cttgttcaat
    24721 gggactttcg tggaagggtt cagtcatccg gaaatggggc atatgttgat caagcgacat
    24781 ccaaaggaca atttttcagg aaactgtccc ttccaccaag attgtttaga ggggatggca
    24841 gcaggaccag cgattgaaaa acggttgggt gtaaaaggcc agaatttatt ggctgacgat
    24901 tcgttttggc agattgaagc cttttacctt gcccaatgtg catacaatac aacactgatg
    24961 ttttcgccag atagaattat ttttggcggt ggtgtaatga aacaagaaca catgaagaaa
    25021 aaagttcagg ataaatttgt tgagcttata aatggatatg ttgaaatacc accaattgat
    25081 agctacatta ttacgcccga attaggtgat aatgccggaa taatcggcgg attagcattg
    25141 gccagaaaag cggtaagaaa taagcaacca taattaatga atgtttcatt gatactcatg
    25201 tcaaaagtat ttaaccgagt aatcaaatga tcaaatgaag ttggataaag cgaaaatgaa
    25261 ttcaaataaa aaggctctaa gtcaaatcgt gttgttttca ttaaatcact gaaaggaaat
    25321 gcttctgcgt ttcctttttt ctttttgttg tgcgctgtat accttgagga tattcactgg
    25381 tggcgtacac ttaaatagta atatattgtg ttactttatg tctgtggtat tagttatagt
    25441 tgcttaccaa tgtatgttca tttttcgaaa tgttgacttc ataatccttg gcttaatgtt
    25501 tgaatttttt tggtgataga ttttttcagc cgtataaaat aaatcgtaat tctattatag
    25561 agagttgtga ttataaaaag atagcaagaa aaaaaaagaa agtttctata cattttaaat
    25621 attttattcc tatttctatt gctaaaaaag tagaaattct atatttacct ttaactctcg
    25681 cttctcttgc aaatgtagtt agcattttaa tcgataataa actcaggtag ttatattacc
    25741 ttgaaatgat tgataatttt gtcacttcta ttttttttgc aataaatttt ttattttagt
    25801 gaaaaataag aaactatttc tttgaaggcg ataagttaga aaaatattat tgtagtcttt
    25861 tctttattag gaatcatcac tgttgtatct tattaccttt tctgtttttc ttattagaga
    25921 aaaataattt gagaggtaga tagtttttat tggaggaagt aattatgtat atttttgaaa
    25981 caaaaatttt tcattgcaat aattgcaaca aagaaatttc atcgggggaa gaggtttgga
    26041 caaaaatgga aatttccgcc aaaagtaact gctcctcaac tgaaatcaat taaagaacta
    26101 gaatttgaga atgcactggt aatttgtatg acatgtactg atgaagtatt tcgaaaacat
    26161 ttttaaaggt tgagatgtat atagatttaa ccaatcattt atctctctcg gaaaaggaat
    26221 attaactaac cagcgggtta tcaaaaatag gtaggaaaac tgtacttaat ggcttatttc
    26281 gcgctgatta tgagaatatc agtctgtttg attaattttt agggttcaaa atacagagac
    26341 aaactccagg tcgggtttcg ccacaagcga attacccgat ctggagtttt gtctattgca
    26401 ggaaggcgta gccggactgc aggaataaag cccccggtt
//
LOCUS       DAJSBN010000033        25975 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJSBN010000033 DAJSBN010000000
VERSION     DAJSBN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.033"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            438..992
                     /locus_tag="ONT86_001656"
     CDS             438..992
                     /locus_tag="ONT86_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2352454.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1165..1638)
                     /locus_tag="ONT86_001657"
     CDS             complement(1165..1638)
                     /locus_tag="ONT86_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2352455.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1650..2186
                     /locus_tag="ONT86_001658"
     CDS             1650..2186
                     /locus_tag="ONT86_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2352456.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2226..2597
                     /locus_tag="ONT86_001659"
     CDS             2226..2597
                     /locus_tag="ONT86_001659"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2352457.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2881..4152
                     /locus_tag="ONT86_001660"
     CDS             2881..4152
                     /locus_tag="ONT86_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR2352458.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4397..5422
                     /locus_tag="ONT86_001661"
     CDS             4397..5422
                     /locus_tag="ONT86_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2352459.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5716..6825
                     /locus_tag="ONT86_001662"
     CDS             5716..6825
                     /locus_tag="ONT86_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR2352460.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6908..7804
                     /locus_tag="ONT86_001663"
                     /pseudo
     CDS             6908..7804
                     /locus_tag="ONT86_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7820..8644
                     /locus_tag="ONT86_001664"
     CDS             7820..8644
                     /locus_tag="ONT86_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2352461.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8676..9986
                     /locus_tag="ONT86_001665"
     CDS             8676..9986
                     /locus_tag="ONT86_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HCR2352462.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10134..11393
                     /locus_tag="ONT86_001666"
     CDS             10134..11393
                     /locus_tag="ONT86_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2352463.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEYRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11403..12275
                     /locus_tag="ONT86_001667"
     CDS             11403..12275
                     /locus_tag="ONT86_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2352464.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12287..13117
                     /locus_tag="ONT86_001668"
     CDS             12287..13117
                     /locus_tag="ONT86_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2352465.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13157..15340
                     /locus_tag="ONT86_001669"
     CDS             13157..15340
                     /locus_tag="ONT86_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR2352466.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15401..16621
                     /locus_tag="ONT86_001670"
     CDS             15401..16621
                     /locus_tag="ONT86_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HCR2352467.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16618..18078
                     /gene="gtfA"
                     /locus_tag="ONT86_001671"
     CDS             16618..18078
                     /gene="gtfA"
                     /locus_tag="ONT86_001671"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR2352468.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18155..19261
                     /locus_tag="ONT86_001672"
                     /pseudo
     CDS             18155..19261
                     /locus_tag="ONT86_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19583..19792
                     /locus_tag="ONT86_001673"
     CDS             19583..19792
                     /locus_tag="ONT86_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR2352469.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19785..19934
                     /locus_tag="ONT86_001674"
     CDS             19785..19934
                     /locus_tag="ONT86_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR2352470.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            20088..20753
                     /locus_tag="ONT86_001675"
     CDS             20088..20753
                     /locus_tag="ONT86_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2352471.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="ONT86_001676"
     CDS             complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="ONT86_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCR2352472.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22456..22689
                     /locus_tag="ONT86_001677"
     CDS             22456..22689
                     /locus_tag="ONT86_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HCR2352473.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22686..23093
                     /locus_tag="ONT86_001678"
     CDS             22686..23093
                     /locus_tag="ONT86_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCR2352474.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24230..24748)
                     /locus_tag="ONT86_001679"
     CDS             complement(24230..24748)
                     /locus_tag="ONT86_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCR2352475.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            25866..>25975
                     /locus_tag="ONT86_001680"
     CDS             25866..>25975
                     /locus_tag="ONT86_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2352476.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         8862 a         3960 c         4830 g         8323 t
ORIGIN      
        1 aattattgac gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
       61 atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt
      121 atagtaaggg gagttcttag tatattgttc aaacttaaat ttcatataat ttaattatgt
      181 aaattagcta ttctaagaac ttctacggat aactgcccgt ctaaaattta aataagagct
      241 tagaatcttc atataagtaa ggttaatttt tttgtacaaa aaagtcggaa tattctgagt
      301 tactgaaatg atataaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt
      361 atatatataa gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt
      421 taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac
      481 aagattttca gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt
      541 tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata
      601 tacgaggcaa aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt
      661 ttatagataa tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt
      721 ggttatttcc gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
      781 atgcagatat agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa
      841 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
      901 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag
      961 catcacttcg tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac
     1021 aaagattaaa agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa
     1081 gctggggtta ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca
     1141 tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca
     1201 aacgctgcgg aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga
     1261 tagtctgctt ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc
     1321 ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc
     1381 gatatgggtg tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata
     1441 cacttctttg acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc
     1501 atgaagtgtg atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac
     1561 atagtctaaa taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt
     1621 tataggtttg attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg
     1681 tgaatcgctt tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac
     1741 taatgaaaac aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc
     1801 accaccgtgt gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt
     1861 ttggaaccaa tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt
     1921 tttgggaaat atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg
     1981 gacgacctat cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac
     2041 gcgaatttga agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc
     2101 atgccatttt aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac
     2161 gagagttatt gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc
     2221 aacgagtgac aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc
     2281 aagatcgtga gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg
     2341 gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta
     2401 taaattcagg aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc
     2461 aattattgat tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc
     2521 aaagtccacg ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg
     2581 aacaagtgga gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat
     2641 aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg
     2701 ttcctatttt tataaactat cattttcgac taataaaact acttatgagt cagcctctct
     2761 ttttttcttg atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg
     2821 tctcattgaa acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata
     2881 ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat
     2941 aaaattagta tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact
     3001 attacccaag aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt
     3061 ttacctaaat acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg
     3121 gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc
     3181 aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat
     3241 gcttattcat ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg
     3301 attgcatttt taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt
     3361 tctgatccat ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt
     3421 tattttgtat tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat
     3481 tggcgttttg aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat
     3541 aacgtttttg atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga
     3601 gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca
     3661 ttggcagtgg aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac
     3721 tacattgtag gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta
     3781 aagaaatatc atttaacctt taaatcgcat tggatatata acaactctga atttgagcga
     3841 atttatcaag aattagctga atttactgca attgttgtta ctggagatta tttagcggct
     3901 aaattagtaa aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt
     3961 ggatttgatg atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta
     4021 aatccaggta acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa
     4081 gaaataaaaa atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac
     4141 aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat
     4201 agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat
     4261 ttactctaaa ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt
     4321 taacgaaatt tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat
     4381 gaaaagagga gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat
     4441 tgcaacagta tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa
     4501 acgaaagatt gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa
     4561 aaatttaaca aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc
     4621 ctattttcga acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt
     4681 gaagaaaatt attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca
     4741 aggtgtgttg attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa
     4801 ttcgaatgtc gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac
     4861 tgatttaaca aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa
     4921 cattgcgttt atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc
     4981 agatgatgat tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa
     5041 atatagtcaa atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt
     5101 acttttgaaa aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc
     5161 tattgctgtg ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat
     5221 ttcgattgtg agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac
     5281 ggtgaatgtg aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat
     5341 aaaagaagtt agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga
     5401 aagtgaaaaa aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt acttttttgt
     5461 ttactaatat ttttagtaaa aaatctcgac aaaacattaa gtaaacagtt atggtgttgt
     5521 taacaaaaag aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata
     5581 gctttaacag ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat
     5641 agtaattcta aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagct
     5701 ccaggactaa gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa
     5761 atggagacaa cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt
     5821 aatatgccag atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat
     5881 gctttgatgg gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact
     5941 gaaggaattg ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct
     6001 tctgtagtgg ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca
     6061 gatgaaacat ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca
     6121 aaaggcatct atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt
     6181 tatcaaaatg ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca
     6241 gaagcacaag aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca
     6301 acaattacag aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca
     6361 atgatgattg aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa
     6421 aatgttgctt ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt
     6481 ggttgggctg ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt
     6541 ttagctagtg aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta
     6601 aaaggctttg aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga
     6661 aaaatgttgg agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa
     6721 gcagaagttc aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc
     6781 gctcaaattg aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac
     6841 aatatacagg cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg
     6901 ggatattatg aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta
     6961 tatttttatt tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca
     7021 aacagcaatt ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt
     7081 aagaaattac caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt
     7141 aatttatgca gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt
     7201 aatgaataga aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt
     7261 tgctgcacca tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact
     7321 gattaataac atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc
     7381 gattgcgatt tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt
     7441 gttattacta gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga
     7501 tggtgctagt ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt
     7561 attttttgta gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat
     7621 gatgattact cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta
     7681 taaacattca tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt
     7741 agtgattata ttaattatta ctgcaattca aatgaagttg cagaaaaagt gggtaaacta
     7801 ttaaggaggc tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat
     7861 gcttatttta ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg
     7921 gaaaacgttg gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct
     7981 aagtaactat caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt
     8041 tttaatggtt gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc
     8101 ttgtgcgcgt ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat
     8161 gatgattccc ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat
     8221 tttaaataca attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt
     8281 attaagacaa tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg
     8341 tgccaatatt tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt
     8401 tgcacttgga atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt
     8461 caatacttca attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata
     8521 tgccacgaac ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt
     8581 gatattcatc gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt
     8641 ataaaactat taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg
     8701 caggaagtac tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac
     8761 aagatgcaca tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa
     8821 tgcttaaaaa tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg
     8881 atcgcaaaga agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt
     8941 ataaaccttc aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta
     9001 ttggtgacac catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt
     9061 tagactttgc taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa
     9121 atccgatggc gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt
     9181 tatgtcatag cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc
     9241 aatatgactt aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt
     9301 tggaaattac aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa
     9361 aaccaaatcc acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata
     9421 ttacagaatc aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc
     9481 cagaattgat tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac
     9541 aaattaatga ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata
     9601 cgagaagccg tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag
     9661 tgattgcagg aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg
     9721 tggaagtacc ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc
     9781 caacacaatt agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag
     9841 ccgctttaac actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat
     9901 catctgaatt gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg
     9961 gggactattt acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc
    10021 ttactctttt cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac
    10081 attagtgtta acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt
    10141 ggtctaaaaa aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt
    10201 gtggaaaaaa tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa
    10261 aagaaatgac tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata
    10321 ttcatgtaaa agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg
    10381 caggcgatgt accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg
    10441 cgaaagctgg ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg
    10501 gatatgcaca acgttttgct attgagtatc gtgtatatag cataccatta accgctaatg
    10561 tctatggttt ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa
    10621 cttgggcaga atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg
    10681 cgattgcagg agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg
    10741 ctgctggtgg tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta
    10801 atgctgaaag caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa
    10861 aagctttgca aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa
    10921 aaggtgatgc gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa
    10981 atccagattt tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa
    11041 cgattggcgc aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag
    11101 ccaaacgttt tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag
    11161 atggtgcacc gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg
    11221 gtttaactga acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata
    11281 gtgaaaatga tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca
    11341 tgattaacgc attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata
    11401 aaatgaaaat aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa
    11461 ttattttgca gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa
    11521 ctaattggac tggtctgact tataattttg attttgtggg tttgaacaat tataagattc
    11581 ttttttctga tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca
    11641 tgattgctgg acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta
    11701 aagggaaaac attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg
    11761 tttctttagt atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag
    11821 gcattgaatt tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa
    11881 tttttgtttt actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc
    11941 aaagtattcc acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa
    12001 ttttttggaa tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg
    12061 cattaaaagg tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa
    12121 gcgatgccac aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt
    12181 ttggttatgc gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg
    12241 cacaattaaa attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa
    12301 agaaaaaaat atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc
    12361 cttagtttca acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt
    12421 tcgttttcct aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg
    12481 tcattacttt tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat
    12541 tattcctatg gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt
    12601 gatgtattta tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat
    12661 taccgttatg atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct
    12721 cacttatgcc gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc
    12781 tatcagtctg gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa
    12841 aattgttttc ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg
    12901 gttttggaat gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac
    12961 actaccattg tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt
    13021 tgcttcctat acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag
    13081 tattatagct ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa
    13141 aggagttcat aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa
    13201 taaaaaaatt agctatctat taggaatcga agaaaataat tatttaacgc atctttattt
    13261 tggaaaaaag attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt
    13321 ctcacccaac aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga
    13381 atttccagga tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa
    13441 cggtagtcgt gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag
    13501 cttagcggga ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat
    13561 tcaattgaaa gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa
    13621 tcacagtgta attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat
    13681 taaccgctta gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt
    13741 aaatggtacc tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa
    13801 agtattagat agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt
    13861 agatgccaaa acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg
    13921 taatcacgaa acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat
    13981 taattcattt aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt
    14041 cgtgatggtt tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta
    14101 taatcaccgt ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa
    14161 ctgggaagca acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc
    14221 gaagaacttg ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa
    14281 tgacttcact tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga
    14341 aaatttagcc aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga
    14401 aatgatttct gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc
    14461 aggtcgtgac cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt
    14521 tcgtgataat atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt
    14581 gaagtgggat atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat
    14641 gcaaggtgaa gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac
    14701 aacgaattat ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc
    14761 aggtttcttg tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg
    14821 tctgaaaatt caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt
    14881 ttcagcagtc ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc
    14941 tgcgatggct ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa
    15001 agcagaaatt aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg
    15061 aactttttat cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc
    15121 aaaagaccaa acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc
    15181 attagtcaca ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt
    15241 aacatttgct ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg
    15301 tgattatgcg acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata
    15361 agtgcaacac caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt
    15421 tactaaaaag cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc
    15481 aatgattaac tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa
    15541 catcaaaaaa aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt
    15601 cattgggccc tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga
    15661 gcggacattt aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg
    15721 tttagatgaa tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac
    15781 ggatattgat gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga
    15841 tatgcgtacc aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt
    15901 atatttagaa aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat
    15961 caatgattat agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt
    16021 tatttatcaa ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt
    16081 tgatgatgta gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga
    16141 gcaagcattg aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg
    16201 gaatacgcaa aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc
    16261 taattatcca gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta
    16321 ttatcatcaa ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg
    16381 acaatttcta ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga
    16441 agataaaatc gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga
    16501 tgaacagtac caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg
    16561 aaaattgcgt ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg
    16621 aaaattaaaa acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc
    16681 gaattaaaac aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg
    16741 cctttttttc catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat
    16801 ccagctcttg gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt
    16861 gattttatga ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac
    16921 catgagcagt caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat
    16981 cgaccaacac aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa
    17041 acggtagaat ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa
    17101 attgatttag atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat
    17161 atggcgaacc atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa
    17221 ttagatacca atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt
    17281 caagaagcag cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt
    17341 caaatgaaaa ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact
    17401 ctgtattcac tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg
    17461 atgaagcagt ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat
    17521 ttattaacag atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat
    17581 gtgaagaagg tctattcaag tgccaattac aataatttag atatttatca aattaacagc
    17641 acctattata gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa
    17701 gtatttgcgc cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat
    17761 attgaactgt tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta
    17821 gccgaaattg aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa
    17881 tttagaaatg aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca
    17941 gaatcagaaa tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg
    18001 gctaacttgg atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt
    18061 gttgcattaa aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga
    18121 tgacttatcc aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc
    18181 atttaataaa tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg
    18241 tatttttgat tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt
    18301 tctctacgca gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga
    18361 ccgatcgctc caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc
    18421 atttaatgca cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt
    18481 tcaacatcaa atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt
    18541 ttcgcttagt ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa
    18601 gttacactta aactattgtt atatggacga tactacgatg tatccaactg attttacctt
    18661 aacaaatgcc catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc
    18721 aacttacgtg tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga
    18781 tggctatttc ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct
    18841 ggaaacatcg ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac
    18901 ctatctaacc gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca
    18961 atttatcacc aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg
    19021 ttggcaaatc gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt
    19081 ttctcaaagt gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct
    19141 cacgtttctc atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt
    19201 ctttcgttat ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg
    19261 agaaaaaaga aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt
    19321 tctggaaaaa gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat
    19381 agttttcaga atattctaaa atgttttatg caacactagt gatatttttt agaattagat
    19441 ttctaatgtg cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt
    19501 aaatttgaat aaaaatatgc aggaacgcta ctatttttag tagtgttctt ttttatattt
    19561 gaataaaaaa tgtggtggtt aaatggtggt tataacaaaa aatgaattaa tgaaattagg
    19621 ttatgggaag tatcagtctg agagaatcat acgacaggca aaagcaatta tggtttcaaa
    19681 aagctatacc tattacgaaa atagaagatt aggactagta cctgttgaat ctgtagaaga
    19741 agttttaggt atgaagatag aggtagagga ggattttcag aatcatggtt aattttaaaa
    19801 aagataaaaa tgggaaatat cattttactg ctagtttggg attcgacaaa cgaacaggga
    19861 aaagagtaca aaaaaacgta gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc
    19921 gaacttgttt ctaattatgg aaaagaagca gaaacttctc aaagtactat gttgtttgag
    19981 gagttttttt ataaattatt cctaccttat tatcaaggga aagtagaaga aagaacatat
    20041 aaaaatcggt tatctacaat caaaaaaact tttcttattt ctttaaaatg gagttaagag
    20101 aaatacgtcc aattgacatt caaaagtggc aaaataattt attgagcaaa tataaaaatt
    20161 cttatgtaag aaacatgtat gggatttttc agatgtcatt agatcgagca gttgttttgg
    20221 gactacttga aaaaaatcct gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag
    20281 ttgatttttg gacaaaagaa gaatttacaa aagttatttc aactttttat attgatgatt
    20341 tgtaccaaca ttttggtttt atttgtatgt ggttactttt tatgaccgtt atgcgtttgg
    20401 gagaagccac tgcattaact tggaaagata ttgattttga aaataaaaac ttgcgtgtga
    20461 ataaaacact ctattataag agagccaaaa cctatgagtt tgtagaaccc gaaacggctt
    20521 ctagtattcg tacaatcgaa ctagacgaca atacaattac ctttttaaaa gcttggaagg
    20581 agcgtcaagt ttcaatagga ggcatgtcgt ttgtactttc ttataatgga acacctactc
    20641 agaaatatac aatccgtcat attatgagaa gacatgcaac gatcgttggt gttcatataa
    20701 tccgcataca cggattaagg catagtcatg cttctttgct tatatcacaa taaaaagatg
    20761 cacttcgaat taaagaaagg ttaaaacatg ctgatgtaca aacgacttta ggaacttatg
    20821 gtcatttgta tctaaattca ttgagagaga taacaaatga tcccacagat tatatccaaa
    20881 ctaatttttc taaaatcaaa ccgacaccta tttacgataa taaaaaataa atgataaaca
    20941 aaagtaggat cacaccaaaa gaaacacact agaaaaacta taatacaggc ttttctagcg
    21001 tgttgtttct tatttccgcc tatttataca ttatactcat aaagcattta atgcatcgat
    21061 cgtagccgca ttgtctatat gggtatataa agttgttgta ttggtacttg aatgtcccaa
    21121 ttgttgggca gttaatacca atgaatttgt ctgttggtag agtctagtag ctaatgtatg
    21181 acgcagtttg tgagggctga cttgaattct aaagcctttt gaatattttg ctaccatccg
    21241 ttcgattgca ataccggata tacgcttggc ttctccttgg taaattgcta gaaataaagc
    21301 tttttcgttt tctgttactt tatatcgttc tttccgaata gctagataat tagctaaata
    21361 ttccattgcg aatgctgcta tattgacagc atctctttta tttccttttc tagttactac
    21421 cacattattc ttatctaaag ataggtcatc taaattaata tttataacct cggagagtct
    21481 caatccagat cctaaaatca gtgcattgat cgccaggtct ctttctacat ctcgttgata
    21541 atagatcaaa gctcgtttac tcaaagtatt tttatattca ttggcaatat aatctagata
    21601 attttgtgta tccttatcta gaaataattt tttctcaatg gcattggcac gtgcagctaa
    21661 cgtagtttta tctgtgatgg ttgctacttg atccatagga ttattgtcta agtatggttt
    21721 tcctagagga ttattggaac ttttcgaaag atatttaaat agaaccttta atgccgtgat
    21781 atttctattg atagtagaag tagatagacc taagttatta ttatctttga gcttagtttt
    21841 tgttgtctct ttttttacat tttcttttgt gcgacgtttc aaatgagatt tgtacaagtt
    21901 taggtcatag gcagttaact gtcttatcga ggtgatagaa gtatcaggaa cgatagactc
    21961 actcagaagc cattcataaa agagcttata attttttaga tattctaaag tggttcgagg
    22021 tgattgatct ttatcctcac gatcttgaac aaaatctttt atataatcgg gcatatgaag
    22081 tatgattgta tctatttttt gatatatttc tgtatttttc atgtcattca tcctttcaat
    22141 aagtagggga gaagagtatc tctataattg gactatacca attatagaat gttttgtcaa
    22201 ttggtatgct ctaaaattta ttaatttgtt tcaaaaaaag catataaaca ggtatacgtt
    22261 taataattta aaataaatgg tatacataaa tagggaagga aatagaaggg agcaatctaa
    22321 attttttgaa ttttcttaat taattatata gaaagaacaa tctttctata gagttagaca
    22381 tgagagggac tatgcttgat ttaaaatcaa caagcgtata tactgtatat acaaagaaaa
    22441 aggagggaga agactatggc aattaaagaa agaaaattta gggaagtggg gaactcagta
    22501 gtattaacta tttcaaagga gtttcttaaa gaaaatgggt taaatcccgg agatacggtt
    22561 tttttagatg aagaaaaact tcgagcggca attacaaaaa aacaagcctt atctgatgaa
    22621 attgatttag caatatctca agcattaact gaatatgata ctacttttag agagcttgtc
    22681 gataaatgag tattcattac ctagaagcca aagatattat aaaaatgaat gtaatccaaa
    22741 taaaaaaata ctctcctaat gaaccccttg gtgtaaagga tgcgaatgct ttagaaatgt
    22801 gtgtagggca acttcgagcc tcagcttttg gtgaagaggt ttatccttcg atttatgaaa
    22861 aagcagctat cctacttata caattgataa agaaacatcc ttttcacaat gcaaataaac
    22921 gtactgcttt cttagctact tttgtctttt tgaaaatcaa tggacagctt ttagctattg
    22981 atcaaaaaga tgctgtaaat ttagcggtat atattgctac gtatgataag gatttcgatc
    23041 aactaaaata tgaagtgatc aacactataa agacacatac gcagcccatt taaggagtta
    23101 ctgtatgtgt ctttataggg ttattagctg ctatagcgaa ataattgaag taaattacaa
    23161 atagctataa ttcaaatcca actatataga aaacgtaaat tttctatata gttggattaa
    23221 gataacgact atttatttta tcatattttt actataccct atacttttac ttggtatatt
    23281 tatgaataat cgcaaattta cccctatact actagtttgt aagttagatt aataaaaata
    23341 ctaaagttat ttgttctttt attaaataca cctacaactt ataaattgat ggtatttaga
    23401 taacacgaaa gaataaaaat aacagctgat agtaaagttt gttgacgatt gctactcaat
    23461 cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact tatgaaatga aagacctttt
    23521 acatcttgca gaaatcggta ctgggattaa actaagcaat aaaaactaat gttcccctca
    23581 ttctttgaac ttctactatt tgatttattt attctgataa atgacgattt caccttcagg
    23641 atcttcaaat cccgtataga atttatttaa gatctcttca gtatcagtaa taggataggc
    23701 gataggccac ataccctttg atggttagaa taaactgctc aaaatctgta tcgtatgttg
    23761 gctttagcat attaacaaag gctatctcca aattataaac cataagcgtt gaatcgttct
    23821 taacaatttc aattcttcct aacccaaagt cagaagatat aattaccgga cttgccttac
    23881 ccttcatgtg ggttgttgag attccatatt taaagggaat tgtcggtact atcggcggtc
    23941 tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta gtctcctaag tccctttgta
    24001 gcggatatgg caccataatc tcattttatc gaagtgagtc atggtttatc tgttggccaa
    24061 ccaaaattga tacaatctaa cgatgcaccc tctacggcat tctaggggag tgcacttgca
    24121 ttcacccaat aaaaaccggc agttagcaaa gaacttatgt agtcgtatgt tcttactgtt
    24181 aacatacaag aggtctcctt tccacaattg cttagcagta aacgggaatt tacaaagcta
    24241 atctcttcca ttttcttatc tttgttctaa aagtcccaaa tggagcaact gtatttacat
    24301 gaataaattt atatacttcc catacagccg ttttggttgc ctcgtctgcc cattttcttt
    24361 tgtgtgcagt gaatagatct tcatcactca ttgaatcaat catcatgtta atatcatcaa
    24421 tattacgatt gagaatttcc tctaattctt tcaatggtag atgtgcatag gtatttgtaa
    24481 accacttata taactctcct aactgattcc atttaaattg atcagaaggc gttttaacag
    24541 ataatccatt tctttcatca ttttcccatt ttagaattaa attcgtccaa ccaacttgat
    24601 atgctaaatt ttcagcaggt gttctatcca cttcctcaac tcttttatcc ttcaattctt
    24661 caggaatagt ttcaaattct aaaatatatt tagtataagt cctatttatt tcttttttta
    24721 attcttcttt atttttatat gttctcatta tattacctcc ataaattcct aattgtctcc
    24781 tttaattcat ctataatttc atccacgata ttttaatccg ttctacaatg catatctgat
    24841 atgcattgac tgtctaactc atatgtgaat gatttataaa atatcccaac gtctcatcta
    24901 aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa tatatttctt ttgtagtaaa
    24961 atcggactgg tttgcatatc attttcttcg taaatatcaa aatcatgtta actactatca
    25021 attactaata aaaactacca ctttaagcgt taagaactgt taagtttagt taagtgagga
    25081 tagttcacaa aatccattgt taaacttcac gaaagtggta aaactcaaaa tgctttatat
    25141 aggattacag catcgctctt tcttcgattg cgcgttgggt aaaaaatatt atgaagtaaa
    25201 aattgacgat gacacgttcc ttacggctca tcagatgaaa cagctacaaa aacgaaatga
    25261 tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca tattcacgcc tcgctcgacg
    25321 aactattaaa ggtcaagatt tacctgaaga tcatcctttt taggaaattg aagcttttta
    25381 tgttgcacaa tgcatttata atactacaat gatgttttca ccagatattc ttctagggtg
    25441 cggtgtaatg aaacagatac acttgcaaaa gaaaattcac cgcgaattta ctaaaattat
    25501 gaataattat gttaatcttc cgaaattttt ttccaaaatt aagggatcaa gctggtgtta
    25561 tagattgttt aaggttggca aaaaaataac ttctttttaa acaattatag aaaaccgata
    25621 ctctgttaag ttggttgaca cggtgtcgat ttttatgact ttcttattct gaaacttcac
    25681 taacggacag tacctaaaaa gtctatagta tggacgttta cgagtactat tttatcaaaa
    25741 atgctgctca tttcaactta tatgttttag tgaactaccc ccacttagct tggataggct
    25801 acaattaact agacagaaaa attaaggtgt gtagactaga agaaaacata ccaggaggaa
    25861 tttttatgtc taagagaaca cgaagaactt tttcacaaga attcaagcaa caaatcgtca
    25921 atctttactt agctggaaag ccacgtgtag aaatcattcg agaatatgaa ctaac
//
LOCUS       DAJSBN010000034        25502 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJSBN010000034 DAJSBN010000000
VERSION     DAJSBN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.034"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            133..3660
                     /locus_tag="ONT86_001681"
     CDS             133..3660
                     /locus_tag="ONT86_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCR2352477.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ONT86_001682"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ONT86_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCR2352478.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ONT86_001683"
     CDS             7448..7789
                     /locus_tag="ONT86_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2352479.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ONT86_001684"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ONT86_001684"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR2352480.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="ONT86_001685"
     CDS             9146..11566
                     /locus_tag="ONT86_001685"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR2352481.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ONT86_001686"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ONT86_001686"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR2352482.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ONT86_001687"
     CDS             13000..13737
                     /locus_tag="ONT86_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2352483.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ONT86_001688"
     CDS             13751..14566
                     /locus_tag="ONT86_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR2352484.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ONT86_001689"
     CDS             14579..15226
                     /locus_tag="ONT86_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2352485.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ONT86_001690"
     CDS             15242..15898
                     /locus_tag="ONT86_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2352486.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ONT86_001691"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ONT86_001691"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR2352487.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ONT86_001692"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ONT86_001692"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR2352488.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ONT86_001693"
     CDS             18240..18758
                     /locus_tag="ONT86_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR2352489.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="ONT86_001694"
     CDS             18865..19365
                     /locus_tag="ONT86_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2352490.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ONT86_001695"
     CDS             complement(19645..20565)
                     /locus_tag="ONT86_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCR2352491.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="ONT86_001696"
     CDS             20730..21743
                     /locus_tag="ONT86_001696"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCR2352492.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ONT86_001697"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ONT86_001697"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR2352493.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ONT86_001698"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ONT86_001698"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR2352494.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ONT86_001699"
     CDS             complement(23383..24144)
                     /locus_tag="ONT86_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR2352495.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ONT86_001700"
     CDS             24333..25427
                     /locus_tag="ONT86_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR2352496.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5384 g         7246 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 gg
//
LOCUS       DAJSBN010000035        25420 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJSBN010000035 DAJSBN010000000
VERSION     DAJSBN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.035"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            12..84
                     /locus_tag="ONT86_001701"
     tRNA            12..84
                     /locus_tag="ONT86_001701"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ONT86_001702"
     tRNA            88..160
                     /locus_tag="ONT86_001702"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ONT86_001703"
     tRNA            168..249
                     /locus_tag="ONT86_001703"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ONT86_001704"
     tRNA            267..339
                     /locus_tag="ONT86_001704"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ONT86_001705"
     tRNA            351..422
                     /locus_tag="ONT86_001705"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ONT86_001706"
     tRNA            439..524
                     /locus_tag="ONT86_001706"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ONT86_001707"
     tRNA            535..608
                     /locus_tag="ONT86_001707"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ONT86_001708"
     tRNA            618..691
                     /locus_tag="ONT86_001708"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ONT86_001709"
     tRNA            711..783
                     /locus_tag="ONT86_001709"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ONT86_001710"
     tRNA            811..884
                     /locus_tag="ONT86_001710"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ONT86_001711"
     tRNA            898..971
                     /locus_tag="ONT86_001711"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ONT86_001712"
     tRNA            995..1084
                     /locus_tag="ONT86_001712"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ONT86_001713"
     tRNA            1097..1170
                     /locus_tag="ONT86_001713"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ONT86_001714"
     tRNA            1189..1264
                     /locus_tag="ONT86_001714"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ONT86_001715"
     tRNA            1266..1336
                     /locus_tag="ONT86_001715"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ONT86_001716"
     tRNA            1350..1423
                     /locus_tag="ONT86_001716"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ONT86_001717"
     tRNA            1448..1535
                     /locus_tag="ONT86_001717"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ONT86_001718"
     tRNA            1557..1628
                     /locus_tag="ONT86_001718"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ONT86_001719"
     tRNA            1651..1740
                     /locus_tag="ONT86_001719"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ONT86_001720"
     tRNA            1753..1826
                     /locus_tag="ONT86_001720"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ONT86_001721"
     tRNA            1845..1917
                     /locus_tag="ONT86_001721"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ONT86_001722"
     tRNA            1921..1993
                     /locus_tag="ONT86_001722"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ONT86_001723"
     tRNA            1998..2068
                     /locus_tag="ONT86_001723"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ONT86_001724"
     tRNA            2081..2154
                     /locus_tag="ONT86_001724"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ONT86_001725"
     tRNA            2166..2237
                     /locus_tag="ONT86_001725"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="ONT86_001726"
     CDS             2503..3696
                     /locus_tag="ONT86_001726"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR2352497.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ONT86_001727"
     CDS             3826..5298
                     /locus_tag="ONT86_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2352498.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ONT86_001728"
     CDS             5381..6169
                     /locus_tag="ONT86_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR2352499.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ONT86_001729"
     CDS             complement(6236..6904)
                     /locus_tag="ONT86_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCR2352500.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ONT86_001730"
     CDS             7019..8731
                     /locus_tag="ONT86_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2352501.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="ONT86_001731"
     CDS             8734..10503
                     /locus_tag="ONT86_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2352502.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="ONT86_001732"
     CDS             complement(10708..11274)
                     /locus_tag="ONT86_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2352503.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="ONT86_001733"
     CDS             complement(11274..12230)
                     /locus_tag="ONT86_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR2352504.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="ONT86_001734"
     CDS             complement(12230..12886)
                     /locus_tag="ONT86_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR2352505.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="ONT86_001735"
     CDS             13256..15274
                     /locus_tag="ONT86_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2352506.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15344..15550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15701..18115
                     /locus_tag="ONT86_001736"
     CDS             15701..18115
                     /locus_tag="ONT86_001736"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR2352507.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="ONT86_001737"
     CDS             18463..20016
                     /locus_tag="ONT86_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR2352508.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="ONT86_001738"
     CDS             complement(20397..22181)
                     /locus_tag="ONT86_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2352509.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="ONT86_001739"
     CDS             complement(22196..23917)
                     /locus_tag="ONT86_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2352510.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="ONT86_001740"
     CDS             24017..24778
                     /locus_tag="ONT86_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2352511.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7872 a         4656 c         5390 g         7502 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    25081 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    25141 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    25201 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    25261 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag
    25321 aaaaatcaca aaggttattg ttatttttat atattctttt taagaatgat tgttttttta
    25381 tagaaatttt aaaaaataaa acacgttatt gatggatagt
//
LOCUS       DAJSBN010000036        25348 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJSBN010000036 DAJSBN010000000
VERSION     DAJSBN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.036"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..258)
                     /locus_tag="ONT86_001741"
     CDS             complement(<1..258)
                     /locus_tag="ONT86_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352512.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="ONT86_001742"
     CDS             complement(274..924)
                     /locus_tag="ONT86_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352513.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="ONT86_001743"
     CDS             complement(935..2107)
                     /locus_tag="ONT86_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCR2352514.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="ONT86_001744"
     CDS             complement(2126..2998)
                     /locus_tag="ONT86_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2352515.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="ONT86_001745"
     CDS             complement(3020..3940)
                     /locus_tag="ONT86_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352516.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="ONT86_001746"
     CDS             complement(3940..4209)
                     /locus_tag="ONT86_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352517.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="ONT86_001747"
     CDS             complement(4206..4586)
                     /locus_tag="ONT86_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR2352518.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="ONT86_001748"
     CDS             complement(5053..6516)
                     /locus_tag="ONT86_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR2352519.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="ONT86_001749"
     CDS             complement(6580..9597)
                     /locus_tag="ONT86_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2352520.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="ONT86_001750"
     CDS             complement(9748..10587)
                     /locus_tag="ONT86_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2352521.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="ONT86_001751"
     CDS             10732..10977
                     /locus_tag="ONT86_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352522.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="ONT86_001752"
     CDS             11040..11462
                     /locus_tag="ONT86_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352523.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="ONT86_001753"
     CDS             complement(11556..11873)
                     /locus_tag="ONT86_001753"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352524.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="ONT86_001754"
     CDS             complement(11900..12106)
                     /locus_tag="ONT86_001754"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352525.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="ONT86_001755"
     CDS             complement(12087..12269)
                     /locus_tag="ONT86_001755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352526.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="ONT86_001756"
     CDS             complement(12283..12633)
                     /locus_tag="ONT86_001756"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352527.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="ONT86_001757"
     CDS             complement(12659..12961)
                     /locus_tag="ONT86_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352528.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="ONT86_001758"
     CDS             complement(12964..13197)
                     /locus_tag="ONT86_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352529.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14183..14505)
                     /locus_tag="ONT86_001759"
                     /pseudo
     CDS             complement(14183..14505)
                     /locus_tag="ONT86_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14628..15743)
                     /locus_tag="ONT86_001760"
     CDS             complement(14628..15743)
                     /locus_tag="ONT86_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2352530.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="ONT86_001761"
     CDS             complement(15749..16147)
                     /locus_tag="ONT86_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2352531.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(17176..17562)
                     /locus_tag="ONT86_001762"
     CDS             complement(17176..17562)
                     /locus_tag="ONT86_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCR2352532.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(17559..18080)
                     /locus_tag="ONT86_001763"
     CDS             complement(17559..18080)
                     /locus_tag="ONT86_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR2352533.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(18082..19116)
                     /gene="sppA"
                     /locus_tag="ONT86_001764"
     CDS             complement(18082..19116)
                     /gene="sppA"
                     /locus_tag="ONT86_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR2352534.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(19149..20564)
                     /locus_tag="ONT86_001765"
     CDS             complement(19149..20564)
                     /locus_tag="ONT86_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR2352535.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(20533..22686)
                     /locus_tag="ONT86_001766"
     CDS             complement(20533..22686)
                     /locus_tag="ONT86_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HCR2352536.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGE
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            22944..23180
                     /locus_tag="ONT86_001767"
     CDS             22944..23180
                     /locus_tag="ONT86_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HCR2352537.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            23199..23414
                     /locus_tag="ONT86_001768"
     CDS             23199..23414
                     /locus_tag="ONT86_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HCR2352538.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(24117..24869)
                     /locus_tag="ONT86_001769"
     CDS             complement(24117..24869)
                     /locus_tag="ONT86_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2352539.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(24884..>25348)
                     /locus_tag="ONT86_001770"
     CDS             complement(24884..>25348)
                     /locus_tag="ONT86_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR2352540.1"
                     /translation="LTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTI
                     IEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLS
                     IDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT         7228 a         5018 c         3849 g         9253 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aagtttgatt ctattaattt ttaaacacca ttcataatat
    16381 atttcctata atgtaaaaag cgtcgatact tattaaagca tcgacgttat ttttatagta
    16441 aatttatcaa aatttttgcc aacatttttt ccgatttttt tccctatttc tatctttcct
    16501 tatctttctt agttaatatt atttgatgtt aatatatcac tactaatatt aaaaaacatt
    16561 gatttatcat aggataaaga gtgatagttt aatacttaaa gagacatatt aattttattt
    16621 aaaaattgac attaatgact cttaatcagc gggtcgcggg ttcgagcccc tcacggccca
    16681 ttgggtgcca aacccacgga acaggtaaat gttggtgcga aagcatttcg aaaacattta
    16741 cgctgttctt tttttatttt tcaaaataac tagggctttt tatttttaac tcaaagtcca
    16801 ctgtagccct ttggactttg agttaaaaaa gaagcataat tatgaacagt aaagtttcta
    16861 atataacaat aattcgagaa gctttgcaaa atcatgctgc caatgaactc tctaaacata
    16921 taggattaaa tctaagtact ataaaaggtt aaaaagtgaa gaatatttaa tagaaaaact
    16981 aaatttactt gctgctttcc ccttttccat taatacaaca atcagagagt gtcttttttt
    17041 cgaaaaaatt agaataaata aaagacacgt agttttttta tctacatgtc ttccatcgac
    17101 tacaagttat catttctcaa aacgtattta gcttttaatt gattaatcgt ttcacgttgt
    17161 ctacacattg cctaattaag attttcttat tagaagcatc aatcccgcaa taatacctaa
    17221 aactccagga attacccaca ataatgttaa aagaaaaaat ggaataatag ataaaatgag
    17281 taaactaata ccatagacca ctgttttttg attttttctt actaaaaaac ctattatgaa
    17341 aatcattatc gaaaaaacta gggtaattcc tccaaataaa acaacattcc cgcttagatt
    17401 gactaacata tctatatcct tactggttag ttctgtagta tttaaagtag ttgaatcaat
    17461 agaagataga aacataccac ccatcaatgc taaaagacta cataaaatcc ctgttattcc
    17521 actaattaaa atcaaaataa actctgtttt tctcttattc atttaattct ctccagatta
    17581 agtgttttag aattagaaat tttttgacca tagttaatag cttctaatga acgaataaca
    17641 accactgacg tgttagaaaa ataatcccca atatgttgtt tcttgggcgt gaaagcagcc
    17701 accaaatacc caatttgaaa tacaaaattt tttaatataa acctacatat acactcacga
    17761 ataaagatag tttgccaact tagctctttt tcctcaaagc tcactactct tatcccaaaa
    17821 atcattttcc caattgtttg tcctgaattc agcttagtca aaagtacaaa ataggttaaa
    17881 taaatagcta atgacgctat accataaaaa gtaataatgg atttgtcaga ttccagtccc
    17941 atgattttgt aaaagagacc tatagtagca tgggtgatga tgctaataca aattaaatca
    18001 ataagaaaag caaaaaatcg taaccaaaat cccgcaaata cataattagg caaagtttca
    18061 atgccactct tcttattcat tttatagccc tccatagtaa tacattggtt ttggagtatc
    18121 agaaaatcca agtttttcaa aaactgaaat tacacgatct gatgtagttg cctttagccc
    18181 ttgccattca gcaatcttat tacctaacca agaagaagca aagccagttg tgtcattttt
    18241 atattctata accgtcgcat cagttaattt cttctctttt tttagggaat ctaatgcatc
    18301 ttcagagtaa ccaatttgat ccaccaaccc attttctact gcttggttac catcataaat
    18361 tcttccatcc gcaagttctt tcactgcatt agtatccata tttcttcctt ttgctacaac
    18421 attgacaaaa cgatcataag cactcatcac aaattcctgt ataactttat tctcttcctc
    18481 tgaaggtttg tgttgaggtg acatcatatc ttttaatgca ccacttttat acgtcgcatc
    18541 agttacgcca agtttttcta ataagccaga ataatttaaa ccagaaataa tgacaccaat
    18601 tgatccagtt gttgtttctt ccgtagcaaa tattttatct gaaccagcag aaatgtagta
    18661 ccctccactt gccgctgtat ttttaaatgc agtatagata ggaatatcta gctttttaat
    18721 ttttgccatt tctttagcaa tctcagcact ttcatatatt cctcccccag gtgaatttac
    18781 ttctaataac actcccttaa ccgctttgtc ttcttgaatt ttctttagct gtgttaaaaa
    18841 attctgatgg ttatattgtt ccctactaaa aagattgctt tcacctgtat ctgcaataac
    18901 tccattgacg gatagtttga caatcttttt atttgatgcg ccttcttcta acgtttcttc
    18961 tgtgatttcg ttagatccgt aaaaaatctt atttattcct gttagggttg tatttgatgc
    19021 ttcctcttta tcttttcctg gaattagcgc gacgataatg gatagtaaca ataatccgca
    19081 cgctactccc acagcaatcc atcttctttt attcatataa actcctccta tattcttagt
    19141 aaaaataatt attttttatt tagaaacaca tcgacaattt gttgccaata tgttttttct
    19201 cctacaatta aagacaactc tccagttaat ccataacgaa acggaaacga ttgagttgtt
    19261 tttaacgttc ctttcactgc ataaaaaaca cctttctcag tggcagtaga attttctgaa
    19321 atttcatcta aagttccgtc gatcgtaatc ggagccccac ctttttgatc caatttaaaa
    19381 tgaaccttca tcccttcttt aatataggta ctttcattcg caggtaaaag tgctgtaaat
    19441 ttcatttttg atttttcgtg gatcggataa atttctgcaa gcactgttcc tttggctagt
    19501 tctgcttgtc cctttgtttc ttcatttaaa tgaaccgttc cagatacagg agcttttagt
    19561 attccttttg aaagttgatc gtccaaactt ttgataactg actggttctt ctcttgtttt
    19621 tcatttagct ctgtaactgt ttgttttaca gttgttattg cttgttctag ctcttgcttt
    19681 cctttttccg tttgactaat tctgtcattc tcagaagtag ttggtttaac taatttagct
    19741 ttttctgact gatactgttc tacttctttt tttagttgct caatttgttc atttatgctt
    19801 gctgaaattg tcagtttcac ttgatttttt tgatcatcag aaacattatt tagttgttct
    19861 tgccaattct catagtttgc catcatttct tttgaaaaat cttttaatga ttgattatta
    19921 ctccaagcta cctgtacttg ttgccattct tgtaatttac tatctgaact ctcaatttgt
    19981 ttttctagtt gtctttttgt cttttcgtat acttctaatt gcttttgatc atttaattca
    20041 ctttgcttca aagcgtagcg gagtgattct ttttctgaca acatgctctt taattgatta
    20101 ctgtagccaa aactgtcatc cgttgaaaac aaattttttc ctttacttat actttcaata
    20161 aatagctgag ccatctttat ttgttgagta atacgttggt tttcttgttc tatctgagcc
    20221 ttttcattct ctattaaaga acaatccaat ctaaccaaca catcgtcctt atgaacaaat
    20281 tgattttctt ttaggaagtt ttccattatt ttagcttcta atggcacttg taacttctct
    20341 attttttcgg ggttcaattg agccgttgtc cgaataacaa cctctttttt agcaaaggtt
    20401 aaaaacaatc caataataca taaaaaaaga attacagggt ataacaccca acggtagaag
    20461 ctatgatgtt gtttagtata gatactagaa tgttcgaacc aattattatt ctgcataaat
    20521 tgtttccctc tcctagattt cccataattt ttgatagata ccatcggcaa aacgaagttc
    20581 atcgtgcgtt cctttttcta ccaaatgccc ttcatggaga actaaaattt gatcacatgc
    20641 ttttgctatt ggtaaatgat gcgcaataaa aataatcgtt ttttctttta aattgaggag
    20701 ataattcata attgcatgtt ctaacaaggt atctaatcca ctagttgctt catctaaaat
    20761 taatatgtcc ggatttttta ataaagcccg tgctaaggct aatcgttgtc gttgctctcc
    20821 tgaaagatta tttccacctt cttccaacaa tgtctcgtaa cgtaatggct gttgattaat
    20881 aaattcattt agttggacta tctcacaaac ttctattatt tgttcgaaac ttggtgggct
    20941 atctaaaccg aatataagat tctcaatgat tgttccacta aagaaaaatg actcttgagg
    21001 caaatatgtc actcgatcgc gtaatatctg aaaaggaatg tctaagtgat taatgtttcc
    21061 ataatggatc gatccttctg atgcttcata aaaacgaaca aataactttg caagagttga
    21121 ttttccagaa ccactaactc caactatagc cgttttactg tgcgaaggaa ttgtacatga
    21181 aatttcgttc aacgttgggg ccttcatgct atacgagaat gaaacgtttt ttacagtgat
    21241 atcttgttga aataattgtt cagaaagcat atattcaaaa gactggttcg atttttctga
    21301 atcaatcgcg aaaatttcat ttaaacgatt atttgcaact tgagccgttt gcattttcac
    21361 ttgtaaatta ataatgtttt gtaaaggatc tgtcaaaaat actagtaaag cattgtatgt
    21421 gataagttga cctagactaa ttctattatc cataacaaaa taagaaccta gccataaaat
    21481 taatgcactg ctaattaatt gaatcaaatg tttaattcct tgttgaatat tatcaagcgt
    21541 aaccgtacga aatgattttt tcattaattt aacaaattca tggtctatac ggttatatac
    21601 tttctcttca ccgctatatg ctttaattgt ttctattcct ttaagacttt ctatgatact
    21661 agaatttaca gctgcaccgg ctgccatttc ttccctgttc gatttttcat agcttttcac
    21721 gaaagcgaat attgcaacta cataaaaagg caaagagcat agagtgatta aaaacaacgt
    21781 actattctga atagctaacg taaccccaac aagcaacacc ataccaatat ctaaaaatat
    21841 tgataacgta gcacttgcta aagcatcaat aatcttactg gcatcaagaa aacgagaaat
    21901 aatttcccct gattttctcg tagcaaaaaa attcattggt aaagttaaca catgtttgaa
    21961 atactgaagc ataattgcca tactcattcg ctgtcctaaa ataataagaa gataactacg
    22021 actatattca aacaataccc gaaataaata gacagcgatc aagccaatgg aaattatatt
    22081 taaagttgaa cgtgcttgat tagggatgaa aaaatcaaga ataccttgaa aataatatga
    22141 gcttcctatg ccaaaaaacg taatgaaaat cgatgctaaa acgatgtgga atactaatcc
    22201 tttttgtttc caaattatag gcaaaaaaga acttaatcca gctacttttt ctttaatagg
    22261 aatataagtt tctctaggca taggtaataa aagaatacct gtccattctt tttgaaacgc
    22321 ttcaatcgtt tttctatttt ttccattagc gggatctgca atccatagta cgtttccctt
    22381 cgttttataa attacaacgt agtgcatata gctgttatca accaatatat gtgcaattaa
    22441 tggtaagata agatctcttt ctttccagat ctcactatct gcttgaatag ccatacaatc
    22501 aaaagacaat ttttcaaatg tctttttcaa tccaaatgct gaagtaccct ctaaatcagt
    22561 cccagatagg tcccgtaatt tatgaatcgg gatatcagtt ttaaaatatt ttaaaatcat
    22621 cgaaaggcaa gcaacaccac aatctttttc atcttgttgc tgcacaaaag gaaatgattt
    22681 tatcacttat ctaacacaac tccttctaaa aaatttttat agaaaaattc tcaatatagc
    22741 tttaatacag tgaaaatatt gtctcataat tccacaaaaa ctttgaaaac agttcttgaa
    22801 cactaacttt ttcattcctg aatcaaattt attttggaaa ttttactatt caagaataaa
    22861 cttgataagt ttcaggaaga tttttactgt taatcaaata cgtatgttac agtagcaccg
    22921 taaaaaatat tatttaaaga agcgtgttta aacatgcaaa atgtaaagga actaagtatt
    22981 gtggaaatgc acaaactgtt ggtggaggca ataatgcttg gggaaaattg gggcaagttg
    23041 taggtggact taccacaggg gcagtaggtg gtgcaggttt aggaacagca atttgcggac
    23101 cagcttgcgg tgttgttgga gggttatatg gagctgtcgc tggaggtgct gcagctggct
    23161 gggatgctcg aaaaaaataa aaaaggagaa taagtagtat gcatattaaa aacacaaaaa
    23221 caacatttat attatcttca gaagagttga aaaatattca aggtgggagt gccgtgggag
    23281 ttttaggcac tactttttct ggggcaacag caggagtaaa actttgttca gccggaggac
    23341 cttatgcaat agctgcttgt ggtgtaggtg gagctttatt aggtgctgga ttcagcatgt
    23401 ggaccggtgc gtaaacaatt aaaaaatatc aaacctcaag attggtttgt gtcgattacg
    23461 aacataggta tgttctttat cttttcaaaa gtttggaatg gacagaactt tttactaaag
    23521 cttcttgtgc tagtttttat aattatatta aatttttatc ttttattaaa gtcagataaa
    23581 aattctttgg aaatgtaatg gtagagatat ttttacaaaa ggacttgcag cgctgtagtt
    23641 ggtgctgcag gaggacctat ttcggccgtt acaggaggta ttggtaatgt acttgtcgga
    23701 tgtgcattaa agcctgttcc tgcaagttaa atttataaaa acaggaaagt aattatctca
    23761 aatcctatca agtacaatca taggattgca cttgacagga caaatttttt aactaaaaaa
    23821 tgaaaaacac tttctaaaaa ttagtagaat ttgccaaatc tttcgcatct tccaccatac
    23881 atttaatttt aattcccctt tatggtactt aataaaaaca agaaaagaac tattcatttg
    23941 gccctttttt ggttccagac catagaaata ttcctccagt aactaatatt gctgttatgc
    24001 acgtaactaa taacagctat aaaaattggt acaactttaa taggattcag aaaaatttgc
    24061 actagtgaag gaatctattt tattagaaaa atgcaatctc cctaaagaaa aaaccactaa
    24121 acagtgcttt ctttcaactt ctccttaatt ttttgtgctt tccctaaagt aaagtttctg
    24181 gccaactctg aatcaaaata tagtgaacgt tttttaaaat ctatttcttt aacgttaaag
    24241 atatttacta aacacgctcg actaattctt gttaacatag gatatttctt ttcaagctca
    24301 ctaattcttc cataaaattc gtactgccca tctttagtac acaatgataa tctatgtggt
    24361 aattttgaag cttctacaaa ataaatgtca tttatatcaa aagtaaatgt ttgagaacct
    24421 atggaaaaaa caaatgcttg attcgtaacc tgttttgttg catctatcct ttcttgcgct
    24481 aataacaata actcaacaat ctcgtttcga tactcatcta gtgtttgatc ttttgttaca
    24541 aatcctaacg tttcaactct tctttttatt gttacgggta acatctcatc atgagtagtt
    24601 gtaaaaatga tttttgcttg aacatcatat tttctgatta cttctgctaa ttctataccg
    24661 ttaacttcat gatttaaatc aatatctaag aaatatattc cttgctttgg tcggaattgt
    24721 ttgaggtatt ttttaacttc taacggactt tgtgttttca atactatctt aaatacttta
    24781 tcgtgaaaaa gaatgaaatt atcaataatt cgttctaact gatttaattg tataatttga
    24841 tcttcacata atattattgg ataactcata aatcctcctt ccctcaaaac aacaaaataa
    24901 cttgagtagt aaattgatac ggatcgtttt gatagttcac aaacatattg ggattctttt
    24961 tattgatttc ctctatacta gataaaccta acccttgatg tccctttttt gttgttattc
    25021 cttttttcat tagagtatct atagagagta gtatctctct acaactattc gatatcgaaa
    25081 tttctatctg tttatcatct cggtaaatag ctagagacag ttctctatct tgactctcta
    25141 tagatgcttc aattgcatta tcgagaataa ttccaagcac cctaatacag tcaaatattg
    25201 gaacaggaac ttcaataata gtacttggac attcgaattt acaacaaatc ttattgttat
    25261 ttgcttggta tagcttcgct atcaacaaac ttttcaaata ttcatttttt atatttttta
    25321 aataacgata ttcaaactta ttggttag
//
LOCUS       DAJSBN010000037        23913 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJSBN010000037 DAJSBN010000000
VERSION     DAJSBN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.037"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            37..510
                     /locus_tag="ONT86_001771"
     CDS             37..510
                     /locus_tag="ONT86_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCR2352541.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            519..746
                     /locus_tag="ONT86_001772"
     CDS             519..746
                     /locus_tag="ONT86_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2352542.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1382..1753
                     /locus_tag="ONT86_001773"
     CDS             1382..1753
                     /locus_tag="ONT86_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352543.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2009..2251)
                     /locus_tag="ONT86_001774"
     CDS             complement(2009..2251)
                     /locus_tag="ONT86_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2352544.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2283..3185)
                     /locus_tag="ONT86_001775"
     CDS             complement(2283..3185)
                     /locus_tag="ONT86_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2352545.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3198..3386)
                     /locus_tag="ONT86_001776"
     CDS             complement(3198..3386)
                     /locus_tag="ONT86_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR2352546.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3400..3963)
                     /gene="amaP"
                     /locus_tag="ONT86_001777"
     CDS             complement(3400..3963)
                     /gene="amaP"
                     /locus_tag="ONT86_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR2352547.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4001..4894)
                     /locus_tag="ONT86_001778"
     CDS             complement(4001..4894)
                     /locus_tag="ONT86_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2352548.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4972..5894)
                     /locus_tag="ONT86_001779"
                     /pseudo
     CDS             complement(4972..5894)
                     /locus_tag="ONT86_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            complement(5943..6293)
                     /locus_tag="ONT86_001780"
     CDS             complement(5943..6293)
                     /locus_tag="ONT86_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2352549.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6326..7228)
                     /locus_tag="ONT86_001781"
     CDS             complement(6326..7228)
                     /locus_tag="ONT86_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR2352550.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7221..8078)
                     /locus_tag="ONT86_001782"
     CDS             complement(7221..8078)
                     /locus_tag="ONT86_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR2352551.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8412..9221
                     /locus_tag="ONT86_001783"
     CDS             8412..9221
                     /locus_tag="ONT86_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2352552.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9261..9758
                     /locus_tag="ONT86_001784"
     CDS             9261..9758
                     /locus_tag="ONT86_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2352553.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10143..10216)
                     /locus_tag="ONT86_001785"
     tRNA            complement(10143..10216)
                     /locus_tag="ONT86_001785"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10180..10182),aa:Ile,seq:cat)
     gene            10403..10726
                     /locus_tag="ONT86_001786"
     CDS             10403..10726
                     /locus_tag="ONT86_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352554.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10890..11144
                     /locus_tag="ONT86_001787"
     CDS             10890..11144
                     /locus_tag="ONT86_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352555.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11214..11459
                     /locus_tag="ONT86_001788"
     CDS             11214..11459
                     /locus_tag="ONT86_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2352556.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11535..11900
                     /locus_tag="ONT86_001789"
     CDS             11535..11900
                     /locus_tag="ONT86_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352557.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(11961..12524)
                     /locus_tag="ONT86_001790"
     CDS             complement(11961..12524)
                     /locus_tag="ONT86_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352558.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(13092..13292)
                     /locus_tag="ONT86_001791"
     CDS             complement(13092..13292)
                     /locus_tag="ONT86_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2352559.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            13688..13837
                     /locus_tag="ONT86_001792"
     CDS             13688..13837
                     /locus_tag="ONT86_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352560.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            complement(14044..14757)
                     /locus_tag="ONT86_001793"
     CDS             complement(14044..14757)
                     /locus_tag="ONT86_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2352561.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(14750..15835)
                     /gene="uxuA"
                     /locus_tag="ONT86_001794"
     CDS             complement(14750..15835)
                     /gene="uxuA"
                     /locus_tag="ONT86_001794"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR2352562.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            complement(15852..16295)
                     /locus_tag="ONT86_001795"
     CDS             complement(15852..16295)
                     /locus_tag="ONT86_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR2352563.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            complement(16329..16682)
                     /locus_tag="ONT86_001796"
     CDS             complement(16329..16682)
                     /locus_tag="ONT86_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352564.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            complement(16794..17195)
                     /locus_tag="ONT86_001797"
     CDS             complement(16794..17195)
                     /locus_tag="ONT86_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352565.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            complement(17232..17741)
                     /locus_tag="ONT86_001798"
     CDS             complement(17232..17741)
                     /locus_tag="ONT86_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2352566.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            complement(17763..18620)
                     /locus_tag="ONT86_001799"
     CDS             complement(17763..18620)
                     /locus_tag="ONT86_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2352567.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            complement(18638..19471)
                     /locus_tag="ONT86_001800"
     CDS             complement(18638..19471)
                     /locus_tag="ONT86_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2352568.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(19485..20282)
                     /locus_tag="ONT86_001801"
     CDS             complement(19485..20282)
                     /locus_tag="ONT86_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR2352569.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(20315..20599)
                     /locus_tag="ONT86_001802"
     CDS             complement(20315..20599)
                     /locus_tag="ONT86_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR2352570.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(20596..21597)
                     /locus_tag="ONT86_001803"
     CDS             complement(20596..21597)
                     /locus_tag="ONT86_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCR2352571.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(21599..22666)
                     /gene="gnd"
                     /locus_tag="ONT86_001804"
     CDS             complement(21599..22666)
                     /gene="gnd"
                     /locus_tag="ONT86_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HCR2352572.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            22665..23513
                     /locus_tag="ONT86_001805"
     CDS             22665..23513
                     /locus_tag="ONT86_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2352573.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT         6958 a         4764 c         3909 g         8282 t
ORIGIN      
        1 tatattttag ctatcaaaga taaaagaggg ataaaaatga aatttaaaac attgctatta
       61 aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact tttttatact
      121 cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt atttgtttct
      181 gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt gaaactattg
      241 aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact aaaaaaaatc
      301 gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa atttatgaaa
      361 gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt cgtattcatt
      421 ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca tgcgatttta
      481 cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc gttaatttag
      541 atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa gtgggcatta
      601 cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt ttttccactc
      661 tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc tataaaaatg
      721 actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat aaaaaataat
      781 ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc agatttttta
      841 tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata caaagggggc
      901 cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt tacgttgtag
      961 ttggatctgc ttttagtata atactttatt cattccacca gactgatagc agtttgagca
     1021 gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag tgcttaaaac
     1081 ttcagctaat ggcccattgt gttaactctt actccattct tttttagtaa gctcccatat
     1141 atactgtaaa gccttctata tattatgtca tgttgctttt taacatttac tatgtagttt
     1201 tgataaaatg atttgaataa cagtgatgga caattctttt ttatttttaa cattattttg
     1261 ataataatga ttatttattt aaaaatacat tagataacat ttgttacgac acgaaaaagc
     1321 ttcacaaaca aatatcgtta ttttaaaatg agggcgaact cacacaatta ggaggaatag
     1381 tatgccattt attgtttact tttttctttc attactcgtg atctatattc cattaccaac
     1441 atttttcctg ctcgtcaatc gtctagcaga gcaacatgat acgattgctg ctcttacgaa
     1501 actttgttta cttctatttg tttgtctaga ttactttacg agctattatt ggctaaaaca
     1561 gacaagagta aaaaagagat attatttctt ccttgtcttt ttcaatctct ttgagtttct
     1621 gatccattgc tttttacttc atcagtatca tacgatccat ttgtggatca tctgggtttt
     1681 caaagtggtc gtattatttt gtatgttcac ctttcctatc tcaagaaaat ttagggaggc
     1741 attgttttat taaagaagct tctctgtatc aataaagaca catttaagga aaaactttta
     1801 aaaatctaat tgggaatttg cccgcataaa taagaggctg tgacaaaagt cttgtcacag
     1861 cctcttattc cgtataaaca gtgttcaaaa gcttgcatct tcgataatat gcccaaccaa
     1921 atgaaaaata tgagaagcta tttttgtttg gctgttctta ttacttgatg aagaacagct
     1981 ttttcacgac ctctttttaa aagtcttttt acagttttcg agcaaagaat gatacgatta
     2041 gtactaaaat gatggcacca atcaatgatg gcacgattgc cattcctgca atacttggtc
     2101 cccaagaacc taataatgct tgaccaagtg ctgatccaac taaacctgct acgacatttg
     2161 caataatccc catcgaagaa cctttattcg tgattgctcc tgcaattgct ccgataattc
     2221 ctccgacgat aagtgaccaa ataaatccca tgtttcgttc ctcctaatgt tttacttcct
     2281 tattactgaa cccgtggttc tgtattttct tttacttttt gtgcgccgct tccagctgct
     2341 gattttactt tatctgtttg attcgaagca aattcaccgg tttttttcgc tgcgtcagtt
     2401 actttgtctt gcaccgtttc ttgatctttt tcaaattctt cttttgtttt gatgtccact
     2461 acgttagcgt tgacttcgat cacttctaaa tgtgtcatat tttttacttc tttgccgatt
     2521 acttcttgca tttcttcaaa gattttttcg atatctttgc catattccac aatgacatcc
     2581 atatcaactg ctacttgttt tttaccgact tctgtttcga tcccagccgt tacattatct
     2641 gtattcacaa gttttcctgc cacattagag aagaaaccac cgtttgcaga taataaaccg
     2701 tctacttgtt caattgcgat tccgatgatt ttttgcacaa ctttatcttc ataattcagc
     2761 tcaccgcgca ttgaatcttc ctgttcagaa tcatgctttc cttggttatt tgtatgatca
     2821 gaaccaagtt ttccttgtcc gtttgtgtgg tcagaactaa gtttttcttg tgctttcgca
     2881 tggtcagaag gatgtttttg ctcttccaca ggtttagaag gatcgattgc atcatgttgt
     2941 tctggaactt tagattctgg agttcctgga tgatgaggtg catgcttttc tgcatcatgt
     3001 ttcttctctt ctactccttg ttgaacgtta gggactactg gatctggatg tgcagatact
     3061 ggtgctgttt tgttcttttc atcgttcaca ccatttgatt ggtttggtgt cgttgggtct
     3121 ggatgtgcag acactggttt ttggttgttc attttattgt tattattttt gttattgtta
     3181 ttcatggatg tttcctccta agatttaaaa tagtgatcta agattttcgt ttgtttcaga
     3241 taaaaaccga tagcgacgcc gatcgctgcc atgatcaaga tcaataaggt cttgaaaaag
     3301 cccacggtga tccataaaac ggctaatatc aatccaagta ccccaaagat gatagggagc
     3361 ttgtatgtcg aaaatagttc tttcattttt ggtccccccc tattctactc gtttgcggtc
     3421 attgcttcgt ttagctggtt cttcaaagcc tgtgtatttc accgatactt tcactggctc
     3481 ttttgttccc aatatgtgtt gtacttcatt ttcgatttct ttcatcaatg ttcctgtttt
     3541 accaatgagt gctgaagttc gtttcaactc cccttttaca ttgacggcga tcttgttttt
     3601 cgttgctttg acatttactt ttggtgaatg gatgaattca tcttcatgca agtgagaacg
     3661 aaccattcct tcaattgctt ttttatccaa cgtcaactcc ccatcaccat gtttcagtag
     3721 aaagtttccg attgtttttg gataaaacag gaaaacaagt aacgtgataa ttgtcaaaat
     3781 agcaaatgca acggcaagcc aaaatacata ttgttgcacg tatgaattag tgatggaaaa
     3841 gaatcgaaac ttctctagct gaaaaggcaa tggataggtt gcacttaata gtatcgcaaa
     3901 caaaagcaca gatagcaaaa gtaatgttcc gatcactccg atcgttttta cacctttatt
     3961 cattttcgca cctcctcata ggggtgaaac gatcaacttc ttataagttg atcgcttctc
     4021 caccagttac tccgtaaatt tgggctgtaa cataacttgc tttattcgat gctaagaaaa
     4081 cataaactgg tgctaattct cctggctgac ctgcacgttc tagtaaactt tgctgaccaa
     4141 attcagggat cgaatcagaa ggttgtcctt cgtctagttg taatggtgtc cagattggac
     4201 caggtgctac accatttaca cggattcctt taggtgcaag ctgttttgct aaaccgatcg
     4261 taaagttagc gattgaagct tttgttgctg cgtaatctaa taaatgttcg cttggattga
     4321 atgcttgaac agaagttgtt gttacgattg ctcctcctgc tgggatatgc ggaacagctt
     4381 ctttcactaa agcaaacata gcgatgatgt ttatcataaa tgtgtctttg acttgctcaa
     4441 gtggtaactc agcaatagac tgacaagaaa tttgctgtcc tgcgtttagt accatcgtat
     4501 ctagcccgcc aagttcactc acggctttat ctatgatttc tttaggtgct tgttcgtcac
     4561 gaagatcaaa aggtagtaag acaatcttac gtcctgcatc tttcacgtat tttgctactt
     4621 cctctgcatc tttttcttct cctgggaaga attgaagagc gatatctgcc ccttctctag
     4681 caaatgcaat cgctgctgca cgaccgatcc ctgaatctcc accagtgatt agtacacggc
     4741 ggttttctag ttgattattg ccttcatagg attcttctcc acaatcagga acaggaatca
     4801 tattctgctg taaagctggt gtatcctgat cttgtttagg aaatttctcc gtatggtaca
     4861 agtttcgcgg gtcgattaat tttggttctg tcattgctgt attcctccta gtttttaaat
     4921 tgtattgcca aaacaaattt ctttgtttga tacctgtttt aaatagattc tttaatttgt
     4981 ataatctttc cggtataaat ggtaacctaa aactactgct gctatcaaag agccaaagac
     5041 tacgagtata aagcctagag cagaactttt acccggtaat cctcctgtat tcattcccca
     5101 aatccctgcg aacaacgccg gaacggaaat gattaatgca gctgaatcta gatatttcat
     5161 gatatgattt agattattgt ccatcatccc gttgaataga tcaccaatac tctctaataa
     5221 atctcgatag atctcgatca ttttttctgt ttgtcgctgt ctcaaatgga catcatagag
     5281 tagctgttca tcgtctaaac gtttgatgaa agtttcagaa ttccataatt gatccattgt
     5341 ttcccgttga tctcttaagg tatgatcgat atatacgatg tctctttcaa gatctgcctg
     5401 tttatacagt tcttcatttt ccgttgtttt acgagctgct ttatccaaag cgtcaattcg
     5461 ctttttcatc tctcttagtt ctttcacgta atgggtgtaa atatctaata atgtatacgt
     5521 gatgatcttt tcaaacgagt ttaaatgatt tccatgcttt gtcagcagcc gatccacaaa
     5581 ttcggttttt ctatcgatac aagtaatcaa gagattatcg gaaagtacaa aagatattgg
     5641 cgttaatttt tttcgatacg attatctgac tgatctagat taatcactac caaagaaaat
     5701 ggatgctcta gtttcgtctc ataaaggcgc tccatccgag aaacttcttc cggatattcg
     5761 gttcctacga aaatatcatc aggaagctcg aattcttcga ttgcttgctc aatctcttcg
     5821 ggctttgcac tatcaagtac gagccaatta tatttttttt catctgtttc tattacattc
     5881 ttttcttcat ccacaaagta gtatctcaat gtatacctcc tttttggact ttctcgaaac
     5941 tattagctga agaagcagtt ctttaatttg atattataat cgagtactgc gatttcattt
     6001 tctaatgaaa agctgtcaag ctgtttgttg aagtccatgt tcaatcgttt accgaaataa
     6061 ttttgctcta atttagatgt atagatgtcc aataatggat gctcgatttt agtcgcatat
     6121 tctccaaatt ccgaaacaaa aggaatgatt tgaaaaaagc tgccttttgc aaaagcacct
     6181 ttgtccctta gaaatggatc ggaatcattg ataagcgcga gtgcgatttt tgtattttcc
     6241 ccaacctttt caattaacgt ctctactgca tttttagaca ttgtaatttc cattcctact
     6301 ccccctcttc agttgttgtc ttctattatt taacaactac atctgttttt ggttgaactt
     6361 ttgcatattt tggatattta gggaatttag ctacacaacg gaaagccgtc atccctgcat
     6421 atttttcgta atatttcttc gttggccctt caaaatcctt gctccacggt ttcacatgtc
     6481 ctgaaaaatg gataatgtaa ggattgttgc gagtttcttc gtattcacgt tcacctgctg
     6541 ctgttggatg tttttttgct tttgccatga tatagccttg tgcattccaa cgcggatgaa
     6601 gtggtaacca acggtcatgt aagatagcat ttaatgcatc ttggtcatgg aatcgtaatt
     6661 tttctggatt atgttcgatg aaatctaata ctttttgcgt aatattttca tctaaccatt
     6721 ttttcacatt gatcagcatc aaacctgagt tgaaataacg cattgattta gctgaaattt
     6781 tcattttttc cagtcgttga tggaacccag cgtcttcaac agctgctacg attgaatcac
     6841 cgaagtcaag acgccataat ttagagatat cttgtaaagc gatcatgtcg caatccatat
     6901 ataagatacg ttcaacttct gtgccgcgga acaattccgg aatcgcaata cgataataag
     6961 ctgtttctgg aatacgatcg ctgattacca catttgtaaa gaattcttta ttgattttca
     7021 agaattccac atctgagttc attcgtgcgt ttttcactga gaagcgtaaa agctgtttac
     7081 tttcaaaatc gatatcatcg tcaatcacat agaaccgaac aaatttagac gggttgcaat
     7141 tatctaatac tgagacaaat aatgcagcta aatgtggtac gaattttgtg ttacaacttg
     7201 cgactactgc tatttctttt ttattcattg atctcgccta ctttcgttag agtactgtgt
     7261 gctaattttt ttaagtataa attggtatac ggatgatctt tcaacgtatt ccaaggttta
     7321 tcatgtcctg taaagtgaac gatcgaaggt ttttcatttc cttctttata ctgacgttca
     7381 tatttttcat ttggtgctgg atgacgttca aaaatcagcg aagtctgcat gttccatttt
     7441 ggatgaagtt gttcccaatc ttcataaagt acagcattca aagcatcttg gtcgtgatag
     7501 atgattcgat cgccattttc actgaggtaa tggatagttt tttctgtaat ttctttttta
     7561 ttccattgat caatgtcaat caccatcact cctgaattga agtacaagtc atcggaatcg
     7621 attcctaatc tttccaaagc tttcacttgg cctggatcga taatagcgcc gattgttttt
     7681 ccatttaacg gctcatcata tagttttacg atatcgtcta gtactaacac atctgaatct
     7741 aagtaaagga ctttcttata atcctcttta gctaataagt ttggtaaaga aattcttaag
     7801 taagcggtag ttgtgatatg gtcactgact aggaagtctt cgtagatatc tttttccact
     7861 gtcaggaatt ggatacttgc attacttgaa tatttattta ccgtttcttc caatccttct
     7921 ttactatatt ctgatagacc atcgtcaatc acataaaatt ttatgcgtct tgttttattg
     7981 caattttcta atgcagttgc gatcatcacg ctcaagtaag gagcataatt ttcgtctgaa
     8041 gcagttacca caggaactac gccgtatctc ttctccatca aaatcactcc tttttctaat
     8101 ctttttctaa cctgttctac atctagaata ttcctcattt ggttttaaag caaaatatat
     8161 gctttgtttt cattgaatat tttaaaattt ttttatcaca aaacgcatcg gtaagcgcta
     8221 actattcgtt tttcatggct ttttattcaa aggtattgac caaaaactac tatatatagc
     8281 cacctttttc ttaagaatgt tttttgaaaa aattcataat gacaaagcaa gttagtttca
     8341 tttttttaaa ttctcattta atcttaaggt aacaggaaaa tagatatgta aaaagaaagt
     8401 aggaagataa aatgaaacta gcagcgattg atttagacgg taccttgctt gatcgaaatg
     8461 gaaagatacc agaagaaaat atccaagcac ttcggaaatt tgatgaaaat ggcggaatcg
     8521 taaccgtcgc taccggaagg aacagcattt ctgctaaaga tattttttcc gaattaggaa
     8581 tcggtggcta tttgatttct tccaatggtt cactgattgc tgaaatgaag gatggaaaaa
     8641 tggcgaaagt tctcagacgt tcaaaaatag atagcgatac actgagaaaa gccttcttct
     8701 atgctcaaga agcgaaaata tccatcattg ctagccgaga aacccaagat gatcagctga
     8761 cattcgatga atcaaatcta gtcaaagatg acccttatta tgaacatttc aatttacaga
     8821 accattcttt tgatcaaatt ttaaatatgt tggaagatcc ttctcttagc tatcttaaat
     8881 tagcattaac agataagagt gaagaaaaac tacagaagct acaaagagat ctgcaagcag
     8941 atggtatcca atcagtattc tctgatccgc atttcttaga aatcacgcct acaggtatca
     9001 ctaaagctca ttcacttgtt tttctggcgg attaccttgg aatcgaatcg agtgacatca
     9061 tggcttttgg cgatcaagaa aacgacattg ctatgttgaa ttacagcggg ttgagtattg
     9121 cgatggggaa tgcacagcga caagtaaaag aaattgcaaa ccaagtaaca ctgacgaatg
     9181 aagaagctgg tgttgcttat attttaaaaa acttctatta aaaagaataa aatgacgata
     9241 tgaatgtagg aggaaagaaa atgttagtta ttattgatat gcaaaatcat attcttgatc
     9301 caaacagcga attttatatt ccagagtcag atcaattggc agaacgtatt gctcaaagat
     9361 tagctaaagc acgccaaaat aatgaatata ttctcttcac acgggatata ccagttgaac
     9421 tgaaggacaa agaagaagaa cgagaagatt tgcaattgat ccctcttctt tctccacaag
     9481 aaaatgaacg agtgattaaa aagtattact ttacgattcc acctgaaact ttagctgaaa
     9541 tccaggaaac tttatttgaa agcaaagaag aacaaaaaga gatcgaagta gtcgggattg
     9601 aaacaaactt gtgtgtactc tctaatctga taggcctgca gagtgctttt cctgaagcag
     9661 acttttttgt tgatccaatg ttagtctcca gtagaaaaca cggagaatcc gctttagagc
     9721 tgttgaaaga tttcaacgtc tctatcgtcg aatcataaaa atatctatct tcatttacga
     9781 tatttaacac acaataaaaa aagcacgcag aactgtttta gtcctacgtg ctttttctat
     9841 caaagtagat tggctttttt atgacacaga gtgaaacggc aggctcacga cctctttctt
     9901 aaatggtgtt caatcagctg accctcggta ttagcttaac aaacggcaaa atatgaggaa
     9961 ctatttttcc gtttgttaag ctaatactca ggtcaaaacg ctgatttcac aacctcttcc
    10021 ttaaacggta ttcaaaagct tgcatctgca gtaataagaa cagccaagta aaaacataag
    10081 aagctgtttt tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct
    10141 tatgtacctt gtaggactcg aacctacgac cggacggtta tgagccgtct gctctaacca
    10201 actgagctaa aggtacatca taaataaaat ttatttttgt tacttttcaa ctatagcacg
    10261 aatgacaaac tctcacaagt ttcaaaatat aagtaaaata atattgttat tcttatttct
    10321 tttacttgtg tcataactat tttgttactt taatttttat ttgcttattt tagaaaaaat
    10381 gttattcttt tatttattct caatgttagg agtgattatt ttgccattta ttgcgatttt
    10441 attcgattta gttgcggcag cagcttattt tctccaatta aattatcaaa cctcaggaat
    10501 tctgtttatt ggaatgattt ttcaaggtgt gattaccctt ttgcttcttg tcatgatgct
    10561 cagctataaa ggaaaaagat atgcccgtat tcaaacaaaa gtatttgtta aatatgtttc
    10621 cattcgctat gggatcatca ttctttcctt tttagtgaat gcgatcgtcc tctttttata
    10681 tgtattgaat tacttagata tcaatccatt gattttttct cgatagataa gacacattca
    10741 gcggaacagt tcctgcttat cagcagaaaa ctgttctgct ttttcatttt tcattgatct
    10801 attcttcttt tattttccta gcatttcatt tgtgatgtat gcaataacat gttatctttt
    10861 attatatgaa tcgttgagga gtccgttaca tgattacgtt ggtgagagtt ttgttttggg
    10921 tgcctgcggt cgcactggtc gcaagtattg tttatcttat gaattggaac aaagaacggt
    10981 tttatttagc tattttgact cttccggcta tctactttat gtggaaagtt tttaattaca
    11041 actactttga acccgatagt gtttttatag aagaattatc aggactggtt ttgtctttat
    11101 tgatcgtcat tctttatttg atacgtttaa ataaaaaaca ttgagtacta ttgattgcat
    11161 gtgtagcttt ctatagtcat tataactatt ctactataat gaaaggtgat taaatgagta
    11221 tcgaagaagc tattttactg gatactttac gttgtcgtct gaatttctta agaactggcc
    11281 gatctttatc tcaagtagct gtttctagcg cgttaagtat tcctttattc aaatatcgta
    11341 agtacgaaga aggagaagaa attccaagca tcattgaatt aatctatatc gctgactttt
    11401 acgatatttc cgttgattac ctaattggtc gctcagaaac agcaagcaaa ctgaagtgat
    11461 tttttccagc cttggtgctg gttttttcat ttaacgtcta aggttaacaa tctatatata
    11521 aaggagacat ttctatgcga ttatggcatg aaaaattgat ttcaaagctt cctcgtcaac
    11581 agcttttagg acaacatcgg gaatgctgtg cattaagagg aaatggctgg ggaaagaaac
    11641 atgctactgt ggattatgtt tttcactatt ctccttatag attattccaa tatcatcagc
    11701 ttgtcatgga tgaaatggaa aaacgtggtt acagcccagc accagagtgg aaaaatccta
    11761 tgtatcgtgg caaatcatgc gaagcatttt ttgatttacc tactgtagag cgcaacgtgc
    11821 cgatttatcc ggaacacgat gaagtttatc tagcagaatg tttagcaaat ttggagcaaa
    11881 aaggaattta tcttacctaa tataactaga aaagccttct atctcctctt acttcattaa
    11941 aaagctttaa ccatcttaga tcaagctaaa atgtcgtaag ttttagggtt atcttcatcc
    12001 tcacgtgcaa aactaaaagc gtactctagt tttggttcat cgccaacttc cctagacata
    12061 aatgaacatc tcctgacgtg gtttaaaagt accgcatctt cgacgtctgt atacgatgca
    12121 cagcacagat tgcttctttt gatctttctt ttgatttttc tgttgcttct tctacacctt
    12181 cttcgattac tacatcgatg cttacaattg cttctgccta atattcccat gcatcgtcga
    12241 tttaagcctc tgtagtcact gtgatcgttt ccacatttta actcctctgt cgtggcttca
    12301 gttgattttg tgatcagttc agttgctaag ccagagtcag aacaacctgc cattgctagc
    12361 gttaatattg atagtaaaaa tagttttttt atcataaggt tccccccctg aaaatagact
    12421 aaaataataa cgctttcttt ttttgttttc agacgaaaat taataatttc taaatattct
    12481 ttaaaaaagc ttataactat tatgcctttt ttaatatata acacagttat ttttaaaaat
    12541 aagaatgcta aatcactcta caataacggc gaaagcacaa aaaataaaaa aattattacg
    12601 ttattaaaaa aacagatgaa agatcaggtt ttattagatc tcattctttc atcacttgaa
    12661 gggatttaat gaactacttt ttcctctttg tcgtttttat atattcgtat tccttcaaac
    12721 agtaggatag cctctttcaa tgcttgatat ttgaactgtc cgttacttaa aaatttagat
    12781 gtgccgccct cttcctagaa catattcttt tttacttttt cattaattga cagaccaatc
    12841 gaacgaaact gtaaatttcg ataggataat gattggtcaa actcattcat tttcaagaca
    12901 tctcgattca tattcaagat aggaatcttt ttatcaacat cactttattc gtattttcct
    12961 ctaccataaa agcttcacgc tccacaaaat tgttttatac actactattc agcccattca
    13021 acgaccaatg aattttttcg tacaaaaaaa ccgtcttttc tttcgaaaag acggttaaaa
    13081 gtcaattttt attaagcttt aacaatgttt gttgcttgag ggccacgttg accatcttca
    13141 acatcgtaag acactgcttg gccttcttct aaagttttga agccgtcacc ttggatagct
    13201 gaaaaatgtg caaatacatc atttccatct tcaccagtga taaagccaaa acctttgtct
    13261 gcgttgaacc attttactgt accgttattc ataaataaat tcctcctgat acgtatcaat
    13321 gtacgttttt tcgttgcaat aaatatttga taggcaaaag gagttttatc tatgtagata
    13381 tatcgctcaa attacgtttc aaaatagatt actcatttaa tatatcatgc tttttctaat
    13441 aacacaagga taatgcaatg aaatataaaa ttcattgtct tctattgtct tcgaatcgaa
    13501 gaaaaagttg aataaccgcg attgattcac tttttgtttt cttgtttatt tgcttttatc
    13561 tgcaactttt gactttaaaa tttcttaact atgaaaacct ttcaattggt ggtatactta
    13621 taaatggaat ttcgttatca acgcgttcgt tgcctttcag caagtattta aagaaaggag
    13681 aatggctatg acattctata acaaagatat acaatatcga gtaaccttgg atacagattt
    13741 gaatctattt gtcgtttttg acaaagaaaa taccaatcat gtagcgacag gtgttacgat
    13801 cgaacaagca gtgcaagaat taaaaaaatc tgcataagga gtggaaaccc tcatatggac
    13861 gattctcgtt cttctcttta aacgcatcta ctggtagata acagtagatg cgttttgtgt
    13921 ttgttctaaa ttgttcacta caaataaggt agatacttca aaataaaaaa ttacttcttt
    13981 ttcatctgtt ccctcagtcg tgcaggacaa taaataaaga ggactagaaa atagtcctct
    14041 ttattagaga tacaaccctt tactttctaa aaatcgtttt gcttgatgca ggtcataaaa
    14101 ttcatagtct gctttattgg attcatataa acttacatct actttcaact tatgcttccc
    14161 attatgatcg ggcaacccta tcaaatgatc tttgatgaga aaagacaaaa tgcccgctcg
    14221 ccttgctgct tcgatccctg caatcgagtc ttcaaataca agtgcttcac ttggaggaat
    14281 attcattttt gaacaagctt tcaaaaagat ctctggttcc ggctttcctt tttttacatc
    14341 atcccctgaa atgatttgat caaacaaggc atatagattt tccattttta aatacatttc
    14401 tacttttacc cgtgtattag acgaagccac gattgtatgg attcctcttt cttttgcaaa
    14461 agataaaatt tcttctgctc cttttttcgt atatactctc ttatcttctt tcacgatttt
    14521 ttctttatat tgattgattg cttctcgcca tttgatatat ggaacatctt ctccgtacaa
    14581 aatgcccatt tgttctctta tcgccatttc tgtttggcca gttagtaaat aatagacttc
    14641 cttacgcatt tcataatcat acttttgtgc tgattttaca taagcacgat aagctaaacg
    14701 tccagtttct aataaaaggc catccatatc aaagatgaag agcttgattt tattcataat
    14761 gctctcccag tggtttttcc ttacttatcg cctcatatag accttgcatg taagtgagtc
    14821 ccattgcacg atcatacagg ccatatccag gcatagccac ttcaccccac acggttctac
    14881 catgatcagg gcgtatagga ccgtcgaacc cttcttgcac caacgctttc ataatatgat
    14941 acatatcaag ggagccttct gtagagggat gtgccgtttc tttgaacttt ctttctccta
    15001 aaaactccac gtttctgaag tggacaaaat ggatgcgcga acctacctca tggatgattt
    15061 caactaaatc gttttccgga tttgccccta aagatcctgt gcacagcgtt agtccattat
    15121 atggagaatc tactgcattt agaattttct tcaagtcgcc aagatttttc gtcactctag
    15181 gaaaaccaaa aagttcccat ggcggatcat ctggatggat agccattttt acatctgctt
    15241 tttcgcacac tggaatcact ttttctaaaa aataagtcag attttcaaat aattcttctt
    15301 cggtcattcc ttcatacagt ttttccagct gagcgaattt tttcaaccgt tcttcttccc
    15361 aacccggcaa tttgaatccc tttgactggc tgtgtatcaa ttgatacatt tctcctggct
    15421 ccatctgatc gacgattttc tgatcgtata aaagagaata gcttccatcg ttgttttcat
    15481 taaacaaatc agttttcgcc catccaaaaa ttggtttgaa gctataacaa atcaatcgaa
    15541 ccccacatcg agacaagttt ttgattgttc gtatatagtt atcaatatag taatctcgtt
    15601 catctgtccc tgccttgatt gcatcgtgga tcgctacact ttcaattcca agcagttcca
    15661 gtccttggct ctcaacagat gctttcaact cctcaatttc tgaaatctcc caaacatctc
    15721 caggcatttt agtcagcaaa gttccaatta tcccatttat tcccggtatt tggcgaacat
    15781 attttaatgg gatcgaatcc cctttttcac cataccatct aaatccccac ttcatctttt
    15841 cagccttcct tctaaaaatc gtcctcatct aatttttttt cttccgctga agcatgccaa
    15901 acagttaaat tatcttttcc ttgtttgaca atttgttcca ccgcttcttc tatcggtgtg
    15961 tgaagcatct gatgattgat tgcttctaac aacattggca gattcacgcc ccctatgaca
    16021 taagtggctg ctttcttttc ttcagtcagt tcattgactg ccaagagcgc ttgattgtac
    16081 ggacttgcgc taattagatc aacaaatact agcatgggtt ctgctgtgtt gatcatagtt
    16141 aatttttctt tgaccttctc tccgaatgtt tggatgtcct ccccagactc taagcacatt
    16201 gtttcaatat ggctcgatgt cccgacaatt acttccgccg catctttcag tccttcgctc
    16261 aacttaccat gtgtaacgat catgatttcc aacattagtc ttctcactcc ttgttttgca
    16321 ctttttactt atcgaaaaag agcaataatc tgattggcat tttgccaatt aaatgttttc
    16381 cctcgtaaat ctgttttggt caaatagaag aactgcgctt gcttttttct tgcactaaaa
    16441 atataattga cgtttttttc tttcaactta tcaacttgtt tcagccactg ttctttattt
    16501 gaaaaataat ttcccgaaaa ctgtagcggt gtatctgccc caacagagtc tagataaaaa
    16561 agaatagcct cttttttcat acccgatctc acgctgtcta agcccttttc tccataacag
    16621 ccttcatcaa caaacgcata cgctacactc ggatcatcaa tcgtacggat catttctagc
    16681 atcgttaata tggatgaagt atttcgaata aaagtttgtc tgctaggcca tccgcgagct
    16741 gcttttccca ataagaaaaa gtagctccca aatgctagta cagtgatacc tgtttatacg
    16801 gatagcagat cgaatacgtg atgaggattt ttgttatata tcatcattcc ccaccaacct
    16861 aaactgaaca acaaaatgaa ggacaataat atagagtaga ttgttttttt cctttggtct
    16921 ttccaatcaa acatgaaata acttcctaat gcatgaacgg gtgtatcgta ataagtacag
    16981 ataactttct ctgctttttc gatatctcca acataaatat tttttgatcg gtaggctagc
    17041 gtatcgtaag caatgactgt cacatctgtt ctcatactgt aaatatctga tactaatgct
    17101 gaaagaaaac gctgtttact cttttttgta cggcgcttac ggtaaatata gcgataacga
    17161 aattcccaat cacgaaacag ctcgttcttc ttcatttttt cgccctcttt atcttttttc
    17221 taagcatttg tttatgacaa atcaaacttg ttcaatacat cttttaatcc tgttttaggt
    17281 gcagatggag tggcttgaaa ataaatattc tttacgccat attgttcatt catttctttc
    17341 aatttttcag catctgattt atttaaataa acagatttcg tcactaaaag atctgtatca
    17401 ggattttttt gagcaatccc tccaatattc aattcttcca tggggattcc gtgttttact
    17461 aagtcgtaaa tcgtttggac tgtttttgcg atcaaaatac aattgtattc tttaaattga
    17521 tattcatccc aatagaattt agctttctct ggtgtaatga caattgtttt ttgtcctgta
    17581 cggcttcctg catttttgta aagatctctc ataaatttat cttttgctac aacttcgtct
    17641 acaacaaaaa tggaattcac tccacttttt tgagataacg ttaccattac ttgcccatgg
    17701 atcaaacgct catccacacg cgctaaattg actacaccca tcttattcac gttcccttct
    17761 ttttataaaa taccgattcc tgctagcaca attaatattc cagtcagcca taataatgct
    17821 tgtgtgactt tcaatccttt tttcgtatag aacaagtaca cacccattac gacagctaaa
    17881 ggtaatatcc ctggcacgat ttgatcaaga atttcctgga taacaaactc acgtccggat
    17941 atcgcaaatt tcaatgaaga ttctactttt acatagtttc ccgcaagcac ccccatcata
    18001 aacaacccta aaactgatag catttcaatc gctgtctgaa caccagtgtt ttgctttagg
    18061 cctgtagcat tacggcccat agtgaaagct tgtcttgcga aaaatacccc tagtaaaacc
    18121 tgatacaacc caaagcagat aaaaggaaaa agtgcgccta aaggacttcc ctgttgtgcc
    18181 caaggtaccg cgattgcgat aaaaatatat tggacggttc ctgaatcgat tgcatctccg
    18241 attcctgcta gtgcccccat caatcccgct ttcgtgttat acatcaagtc atcctgcatc
    18301 aagatctctt tttcttcata ctcttcttct gctctttgct gttccataga agacattagg
    18361 ccagtaatca caccgccacc ccagcttaat tgagtgttga agaacagtaa ctgccgttta
    18421 tatgctgctt ttaaagagtc ttcatcttta taaagttttt tcaagattgg catcattccg
    18481 tatagtagtg atggcgccaa gtaacgttca aaagagtgag gaatttcgtt tgcaaaatgc
    18541 catctcagat aggcttttgt tacatctttt ttcgtgattt cttcaggagc acctgtttga
    18601 ttatccatta tttcagccat ttcccattcc tcctaaatta aaatccgtca tcatagtcat
    18661 cttcgtcatc aaaagatgct gaattgttat tgcttgtggc aggctgtact tgtttccctt
    18721 tttgcgactg gacaaagatc aaagcgataa taatcccaaa aatggcataa gtaaccatcg
    18781 taatatctaa agatttcaaa acaacactca taaaataagc aaggaagaaa aagaccagat
    18841 aatcttttcg accgataacg tatacagttg tcgcgatacc gattgccgct aaccctccac
    18901 ctactacttc aagaatgtgg ataacgactg ttccctctaa agctttgatt acatcagcga
    18961 tcaatggcgc ccctaaatat aaagcaatga aaacaagcgg aacgaataat aaaaatgagg
    19021 caaccgttgg ataaagaata attgatcggg ccatagcttt tgtattcaaa tcttctacag
    19081 ctttgtctgt atatttacct aaaaaagtat tgatgaaaaa acgaaactga tacaagtaac
    19141 ttccgagcaa tccgactggc accgcaacgg caatcgctgc ttctggcgac agatccccta
    19201 ataatgcgac tggtactgcg atagctgctg caatagatgg ttcagctggc attgatcctc
    19261 ctggagaaaa aacccccata taaatcattt gcaaagcggc tgtaacaatc atcgcttgtg
    19321 caacttcccc catgatcaac ccgacgacta atccagtcat caaaggagaa aaacgtaagg
    19381 tcaacgtggc tcctccacct aaccagcggg acataaccgc ggctacccat aaagcaatca
    19441 acaaactttg tactacactt aatgtttcca tgtgtcctcc tccttcagat ctttctgata
    19501 taaccttcca gttccatcat tgactgtatc agtgatttct gtgttacctc tataggaatc
    19561 aaatgcactt tctctttcga atcaataaat tcagccattt tatctattat ctgtttatct
    19621 ttcggaaata atcccatttg ttctagagaa accggcagtt ctaattcccg atacaaagga
    19681 tataactcgt ctattttttc ccagcgacct tctagtgcca gttgatagaa aatcccatac
    19741 gctacttttt ctccatgtag aaaacgatgg ctttccggaa tatatttact gatagcgtca
    19801 tgcatagcat gtgctgccac atttcttgcg tatttatctc ctaatcctcc aactaaacca
    19861 gcaacagcaa agataatctc tgaaagctgt ttgaactccg atgtcagttt tctttgtgcc
    19921 atatcttgga ttgctttagc agaatgattc aaaatctctt ctttgcagat cttcgcagta
    19981 tcttgtgcta gcttcaaaaa tgattcatct tgcaaaaaat cttgttctaa gatgagctca
    20041 gattcatacc attttgctag tgtatctgct aacccggcaa tgaaatatgg aacaggggaa
    20101 tcaatcacta attggggatc tgttataaaa aaagcagctt gtctgtaaaa gtgttctgtt
    20161 ttcccttctg cttgtccatt ctccttatac atgacagcaa gaggtgtcca tggagcacaa
    20221 ttactagcta aagtagggat gactccgaaa tcgattcttg caagattggc actataaccc
    20281 actagatcgg tcagttttcc tccgccaacg ccaactacga aatcgatttc atttttttga
    20341 tgattgtagc tattcgtctg gcttcagcat aactacattc tccagaataa cgatgataaa
    20401 aaaattgaat cgtttcgtca ttcaaaaata ctaatttagg tagcgccttt tcccatgata
    20461 ccgttccgtg ggtgaccagt actcttttcg cgccgtatga tttcaagaga tcagaaactt
    20521 ttgaaatacc accttcttca tataaataaa attgagggcc tgttttcact tttaaaccgt
    20581 tcaccatttt cttcatcatc cttttatata gttgctcttt gtctggacag aacgttcagg
    20641 aagaaggtgc tgactgatct gttcgacatc tctgacacat ttttcaccca ttgcttctaa
    20701 acattccctt gtgtaggccg atatatgtgg cgtaatcact acttgttcaa acgctaaata
    20761 cggatgatct tgacgtcccg gttcttcttc taaaacatct gtggcatatc ctgcgatctt
    20821 tccgcttttc agaccattaa tcatcgcttc ttcatcaact agagcgcctc ttgcactatt
    20881 agatagataa acattttctt tcatctgatc gacttcttta tcagaaatca tgtgatagtt
    20941 ctcttctgtc aaattcgcac ataaacaaat gatatctgat tgttccagta attcttgaag
    21001 ttctactttt tttgcaccaa agctttcgat gtataaatct gatttgtaag gatcataagc
    21061 tagaacatca cagcgaaaac cgttacgcaa aatttcgatt actccgcttc cggtatttcc
    21121 tgcaccaatc acaccaactg ttttgttaaa cagcgtccta ccgacgaact ccgcccgatc
    21181 ttcccatcga tcgtccttga ctcttgcttg tgcttgtgtc gtgcgtcgaa gcatggacaa
    21241 caaattagtt atattatttt ctgctacagc atttcgttca actaaagcag gtatgataga
    21301 tacgattgtc tggtgttctt ttgctgcttt taaatcaata ttgttgtaac caataccatg
    21361 tcgggaaatc aatagcagtt catctttgtt ttcaaaaaaa tctcgtcgaa aaaatggcgt
    21421 aacacttgca ataatgatat tgaatccttt aagtaactcc gctagttctt ttcctggaat
    21481 ctcttgatca acagtaaaga aatgaacgtc tccaactgac tttaatcttt ccaagtggtg
    21541 tggaaagact cttccgaaac tgctggaatt aacgattgct atatgataat ctggcatttt
    21601 actcaccctt ttcaactatt tcatgtccgc caaacccatt tcttagtgct gaaactactt
    21661 ttgctgaaaa actatcttct tcctgtgaaa gattcctcat gaacaaacta gttgcaatta
    21721 ctggaactgg aacagccatt tctagcgctg tttcaaccgt ccattttgct tcacctgatg
    21781 cagaaacgat tccccgataa ttacttaaat tgggatgttc gtttaactga tcttccatga
    21841 tttccatcag ccagccgcga atcaccgaac catgattcca aacacgtgcg acttgttcca
    21901 agtcaaaagc atactcactt ttttgtacaa gttggaatcc ttcaccgatt gcttgcatca
    21961 ttccatattc gattccatta tggatcattt tcaagaagtg tccgcttccg gatgcaccag
    22021 catacagata tcctctttca attgctacat ctttgaataa ttgttccacg gatagaaaag
    22081 tttctttatc tcctccaatc attaagcagg cttgatgtcg tgctccttcg attcctccag
    22141 aagtcccaca atccaagtaa ccgatttcat ttttctgaaa ctcttctgct cgacggattg
    22201 aatctttata ataagagttt ccgccttcaa taataatgtc tcctggtccg agttttggaa
    22261 aaagttttct taacatgttt tcggttatct ctccagcagg aagcattaac cagatgattt
    22321 ttttctgctc aaaagtagct agaaattcag agatctcttg ataagcagga atgtcatttt
    22381 ctcttgcctg actaacagct gtacttgaaa tgtcgagtcc ttgtactttg tgatggtgct
    22441 cttttaagtt taaagctaaa ttcaatccca tttttcccaa accaattaca ccaatttcca
    22501 tgatagcgcc tccatttctt ataccctcat tatacaaaaa aatttttttg taaatagatt
    22561 ttacaaaaat attttttgtt tgattttttc ctactctcct tgtatcttta acattaatga
    22621 gaacggaaaa aatgataatc ttattatgaa aatgaggaat tctcatgagc gtctccataa
    22681 gactacaaat caaaacaatg tacaacgact tcagcccaaa agaacaggct atcgccgatt
    22741 atattttaga aaatccaagc aaagtaagtc atagtcctat tagcaattta gcaagtgaac
    22801 tgggcattgc tgactctact ttttttcaat ttacaaaaaa acttggttat aatggattta
    22861 aagattttaa aatggccatg ttgatccaag aaaatgatct atctgctatt tctatccacg
    22921 aaaatattca gaaaacagat actgagctca ctatggcgca aaaggtgttc gagtctagta
    22981 tttcaacttt aaatgacaca aaaaagttgt tacaacaaaa ggatctaaaa aaagcggcta
    23041 ctattatcaa tgaatctaaa cgtgtctatt ttttcggtgt tggcggttca gagattgtag
    23101 caactgacgc ttatcataaa tttttgcgtt cccctatcgc aacttctcac agcacggatt
    23161 accatattca attgatggaa gcttctttat tgacggaaaa agactgtgct gttttgattt
    23221 ctcacacagg gcaatcaaaa gaaacaatcc atattgctga aaccgtaaga aaaactggag
    23281 caaaaacaat cgttattact agccaagcta attcttccct tgctaaactt ggcgatgtcg
    23341 tttttatttc tatttctgaa gaaacagaat tccgatctga agctttagct tcccgtattt
    23401 ctcaactaag cattttggac tctctatatg tgattttgat gttttacaac gaaaaagaat
    23461 cacgtcgttc tatcgctaaa gttcgacata ctatttctga aatcaaagaa tagcaccata
    23521 acagaaaaag cgaaaaatcc ataaaacaaa aaaagccact accaaatatt tggtagtggc
    23581 ttttttggtt ctgcaagggt ggtacagaac atcaatagtc ggtgggttag aacgtatgtc
    23641 atcgcatctt tggagaaggt tgtattggta ttattcgagt tacttgttag atgcgataac
    23701 aaagttagta tacagtagta aaaaatttat cttttctttt ttttattatt tttgtttctt
    23761 attttctaaa aaattaaaaa aaattttggt gaattaagta tttccttgat aacactggct
    23821 tttggcctca ttttaggtta tcatttgtaa ttttatttca cagttgatat gattgttatg
    23881 taaaataaaa aagtttagga tacatcgaat ttt
//
LOCUS       DAJSBN010000038        23831 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJSBN010000038 DAJSBN010000000
VERSION     DAJSBN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.038"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(55..789)
                     /locus_tag="ONT86_001806"
     CDS             complement(55..789)
                     /locus_tag="ONT86_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR2352574.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ONT86_001807"
     CDS             1116..1526
                     /locus_tag="ONT86_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR2352575.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ONT86_001808"
     CDS             1519..2220
                     /locus_tag="ONT86_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCR2352576.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ONT86_001809"
     CDS             2220..3806
                     /locus_tag="ONT86_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2352577.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ONT86_001810"
     CDS             3825..4046
                     /locus_tag="ONT86_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCR2352578.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ONT86_001811"
     CDS             4043..4804
                     /locus_tag="ONT86_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCR2352579.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ONT86_001812"
     CDS             5184..5693
                     /locus_tag="ONT86_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR2352580.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ONT86_001813"
     CDS             complement(5768..6307)
                     /locus_tag="ONT86_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCR2352581.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ONT86_001814"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ONT86_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR2352582.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ONT86_001815"
     CDS             7320..8171
                     /locus_tag="ONT86_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR2352583.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ONT86_001816"
     CDS             8199..8834
                     /locus_tag="ONT86_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCR2352584.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ONT86_001817"
     CDS             8854..9627
                     /locus_tag="ONT86_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2352585.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ONT86_001818"
     CDS             10035..10832
                     /locus_tag="ONT86_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCR2352586.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ONT86_001819"
     CDS             10810..11223
                     /locus_tag="ONT86_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR2352587.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ONT86_001820"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ONT86_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR2352588.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ONT86_001821"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ONT86_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR2352589.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="ONT86_001822"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="ONT86_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCR2352590.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ONT86_001823"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ONT86_001823"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCR2352591.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ONT86_001824"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ONT86_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCR2352592.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ONT86_001825"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ONT86_001825"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR2352593.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ONT86_001826"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ONT86_001826"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR2352594.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ONT86_001827"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ONT86_001827"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR2352595.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ONT86_001828"
     CDS             21907..22485
                     /locus_tag="ONT86_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2352596.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ONT86_001829"
     CDS             22537..23019
                     /locus_tag="ONT86_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCR2352597.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ONT86_001830"
     CDS             23136..23510
                     /locus_tag="ONT86_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352598.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4285 c         5114 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DAJSBN010000039        23261 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJSBN010000039 DAJSBN010000000
VERSION     DAJSBN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.039"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ONT86_001831"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ONT86_001831"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR2352599.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ONT86_001832"
     CDS             complement(1172..1867)
                     /locus_tag="ONT86_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCR2352600.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ONT86_001833"
     CDS             complement(1880..2596)
                     /locus_tag="ONT86_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCR2352601.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ONT86_001834"
     CDS             2926..3534
                     /locus_tag="ONT86_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCR2352602.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ONT86_001835"
     CDS             3859..4182
                     /locus_tag="ONT86_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCR2352603.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ONT86_001836"
     CDS             4364..5734
                     /locus_tag="ONT86_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCR2352604.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ONT86_001837"
     CDS             complement(5864..7165)
                     /locus_tag="ONT86_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR2352605.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="ONT86_001838"
     CDS             complement(7519..9477)
                     /locus_tag="ONT86_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR2352606.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ONT86_001839"
     CDS             complement(9654..9920)
                     /locus_tag="ONT86_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2352607.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ONT86_001840"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ONT86_001840"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR2352608.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ONT86_001841"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ONT86_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR2352609.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="ONT86_001842"
     CDS             13264..13827
                     /locus_tag="ONT86_001842"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR2352610.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ONT86_001843"
     CDS             complement(14080..14601)
                     /locus_tag="ONT86_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCR2352611.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ONT86_001844"
     CDS             complement(14604..15446)
                     /locus_tag="ONT86_001844"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCR2352612.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ONT86_001845"
     CDS             complement(15439..16164)
                     /locus_tag="ONT86_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR2352613.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ONT86_001846"
     CDS             16340..16636
                     /locus_tag="ONT86_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352614.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="ONT86_001847"
     CDS             17204..18796
                     /locus_tag="ONT86_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR2352615.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ONT86_001848"
     CDS             18809..19549
                     /locus_tag="ONT86_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2352616.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ONT86_001849"
     CDS             19717..21066
                     /locus_tag="ONT86_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2352617.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ONT86_001850"
     CDS             complement(21194..21784)
                     /locus_tag="ONT86_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR2352618.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            22020..23099
                     /locus_tag="ONT86_001851"
     CDS             22020..23099
                     /locus_tag="ONT86_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2352619.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         6661 a         4820 c         4527 g         7253 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaggcggat tttataatta acttagccat ttggaaataa aaaaacgcca
    21961 cgtgaattcc atgttatatt gaagttacca caactctcaa tagacaagga tgaattcaca
    22021 tgacgcaaaa ccatgataac acagtttcac gcaagggaaa acacctaacc tactcagaac
    22081 gttgccaaat cgccatcctg aaaaaggaaa actattccaa ccgtcaggtt gcggatgtcc
    22141 tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg cgggaccgtt actcagctta
    22201 accgccagaa tcagaacggg aaggtctacg actattatca tgacgtctac gacgccgatg
    22261 ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg cagacggccc ttatgggccg
    22321 acacggacgc cttcattgaa tgggcggacg acatgatgct gttggaaaaa tggtccccgg
    22381 acgtggtggt gggctttgcc agaaaacacg aattgttcga cccggccatt atccctggga
    22441 ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa aacgaagaat atggacctgc
    22501 tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg caaccgcccg aataaacggg
    22561 tgctagggaa atccattgaa gagcgcccgg aggcggttgg tacccggaaa accttcggcc
    22621 attgggaagt cgacacggtc gtaggaaaca agaccaagtc ggatgccgtg ctgctgacgt
    22681 tggtggaacg gcagacacgc ttcgaggtca ttctgaagct ggccgggaag gatgccgaat
    22741 cggtcaacca agcggtccga cagctgagag aacgggctgg tgcgtacttc ggccagctgt
    22801 tcaagacggt tacctcggac aacggctcgg agttcgcggg actgcacgat gccttgcagg
    22861 atgtggcaaa catctacttc agccacccct atgcttcctg ggagcgcggg acgagcgaaa
    22921 accaacacaa gctgattcgc cgtttcatcc ccaagggcag cccaatcggc aacgtgagcg
    22981 aaaaccaaat cttgcggatt cagcgttgga tgaacgatta cccgcgaaag attttgggct
    23041 atgcaacgcc gcatgacacg ttcgtccagg catttaggca ggaacggttg acggcttgac
    23101 acgtgggccc ttgacaacga gcctccctcg gcatggcttg tgtctggaaa gctggccggg
    23161 gccagccagt cgggagacta ccatgcctca gctcattgtc aagggcccac tcgattagaa
    23221 aaattagacg gttggtggta acgagcaacc gggtcaccat g
//
LOCUS       DAJSBN010000040        23065 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJSBN010000040 DAJSBN010000000
VERSION     DAJSBN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.040"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(109..564)
                     /locus_tag="ONT86_001852"
     CDS             complement(109..564)
                     /locus_tag="ONT86_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352620.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="ONT86_001853"
     CDS             complement(718..1926)
                     /locus_tag="ONT86_001853"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCR2352621.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="ONT86_001854"
     CDS             complement(2038..2370)
                     /locus_tag="ONT86_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR2352622.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="ONT86_001855"
     CDS             2518..3393
                     /locus_tag="ONT86_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2352623.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSGELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="ONT86_001856"
     CDS             complement(3475..4056)
                     /locus_tag="ONT86_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCR2352624.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="ONT86_001857"
     CDS             complement(4062..5321)
                     /locus_tag="ONT86_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR2352625.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="ONT86_001858"
     CDS             complement(5323..6369)
                     /locus_tag="ONT86_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352626.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="ONT86_001859"
     CDS             complement(6604..6879)
                     /locus_tag="ONT86_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCR2352627.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /gene="der"
                     /locus_tag="ONT86_001860"
     CDS             complement(7122..8432)
                     /gene="der"
                     /locus_tag="ONT86_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR2352628.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="ONT86_001861"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="ONT86_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR2352629.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="ONT86_001862"
     CDS             complement(9978..10658)
                     /locus_tag="ONT86_001862"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR2352630.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="ONT86_001863"
     CDS             complement(10707..11342)
                     /locus_tag="ONT86_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2352631.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="ONT86_001864"
     CDS             complement(11408..12811)
                     /locus_tag="ONT86_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR2352632.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="ONT86_001865"
     CDS             complement(12798..13829)
                     /locus_tag="ONT86_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2352633.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="ONT86_001866"
     CDS             13860..14081
                     /locus_tag="ONT86_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCR2352634.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="ONT86_001867"
     CDS             complement(14246..14983)
                     /locus_tag="ONT86_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCR2352635.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="ONT86_001868"
     CDS             complement(15136..16476)
                     /locus_tag="ONT86_001868"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCR2352636.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="ONT86_001869"
     CDS             complement(16713..18236)
                     /locus_tag="ONT86_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCR2352637.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="ONT86_001870"
     CDS             complement(18614..19225)
                     /locus_tag="ONT86_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2352638.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="ONT86_001871"
     CDS             complement(19555..20271)
                     /locus_tag="ONT86_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR2352639.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="ONT86_001872"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="ONT86_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCR2352640.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="ONT86_001873"
     CDS             complement(20830..21627)
                     /locus_tag="ONT86_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCR2352641.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="ONT86_001874"
     CDS             complement(21597..21968)
                     /locus_tag="ONT86_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR2352642.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="ONT86_001875"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="ONT86_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR2352643.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6467 a         4926 c         3911 g         7761 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctggtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc
    23041 ccattttcat tttaggtatt aaaaa
//
LOCUS       DAJSBN010000041        22535 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJSBN010000041 DAJSBN010000000
VERSION     DAJSBN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.041"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..497
                     /locus_tag="ONT86_001876"
     CDS             <1..497
                     /locus_tag="ONT86_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2352644.1"
                     /translation="FNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKAL
                     DIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNE
                     NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFY
                     SSLI"
     gene            complement(568..1878)
                     /locus_tag="ONT86_001877"
     CDS             complement(568..1878)
                     /locus_tag="ONT86_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR2352645.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1890..3308)
                     /locus_tag="ONT86_001878"
     CDS             complement(1890..3308)
                     /locus_tag="ONT86_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2352646.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3548..5116)
                     /locus_tag="ONT86_001879"
     CDS             complement(3548..5116)
                     /locus_tag="ONT86_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR2352647.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5120..5401)
                     /locus_tag="ONT86_001880"
     CDS             complement(5120..5401)
                     /locus_tag="ONT86_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCR2352648.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(5398..6321)
                     /locus_tag="ONT86_001881"
     CDS             complement(5398..6321)
                     /locus_tag="ONT86_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCR2352649.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6318..7196)
                     /locus_tag="ONT86_001882"
     CDS             complement(6318..7196)
                     /locus_tag="ONT86_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCR2352650.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7183..8520)
                     /locus_tag="ONT86_001883"
     CDS             complement(7183..8520)
                     /locus_tag="ONT86_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR2352651.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8523..9626)
                     /locus_tag="ONT86_001884"
     CDS             complement(8523..9626)
                     /locus_tag="ONT86_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR2352652.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9700..11208)
                     /locus_tag="ONT86_001885"
     CDS             complement(9700..11208)
                     /locus_tag="ONT86_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2352653.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11174..11866)
                     /locus_tag="ONT86_001886"
     CDS             complement(11174..11866)
                     /locus_tag="ONT86_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2352654.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11888..12862)
                     /locus_tag="ONT86_001887"
     CDS             complement(11888..12862)
                     /locus_tag="ONT86_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2352655.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13162..14034
                     /locus_tag="ONT86_001888"
     CDS             13162..14034
                     /locus_tag="ONT86_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2352656.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14616..16814)
                     /locus_tag="ONT86_001889"
     CDS             complement(14616..16814)
                     /locus_tag="ONT86_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2352657.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            17046..18038
                     /locus_tag="ONT86_001890"
     CDS             17046..18038
                     /locus_tag="ONT86_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2352658.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            18098..19519
                     /locus_tag="ONT86_001891"
     CDS             18098..19519
                     /locus_tag="ONT86_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR2352659.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19512..21011
                     /locus_tag="ONT86_001892"
     CDS             19512..21011
                     /locus_tag="ONT86_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR2352660.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            21050..22444
                     /gene="uxaC"
                     /locus_tag="ONT86_001893"
     CDS             21050..22444
                     /gene="uxaC"
                     /locus_tag="ONT86_001893"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR2352661.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6839 a         4589 c         3905 g         7202 t
ORIGIN      
        1 atttcaattc tgagtttggt caccttgaag gtgatacgat cgttggcatt catcataaaa
       61 gtgccgtcat tactttagtt gaaagattat ctaaagtcat tatcacgatt aaacccaacg
      121 gccgtaaggc attagatatt gaaactgccc ttaatcaatg gttttctcgc ttccctaaaa
      181 acttctttaa atctattacg tttgactgtg gaaaagaatt ttctaactgg aaagccatta
      241 gtaaccaaca tgatattgat atatattttg cggaccctgg aacaccttct caacgcccat
      301 taaacgagaa ttctaacggg attctgcgtc gtaatggact gccgaaatca atggatttta
      361 gaaaagtgaa tcagacattt atttccagtg tcagcaatca acgtaatcat attccaagaa
      421 aatcattgaa ttacagaaca ccaatcgaaa tatttttgag ctatgtacaa gaagcatttt
      481 attctagctt aatttgacaa atcatatatc attaaaaaaa ataaaggtca agctaaaata
      541 ttatttaagc ttgaccttat tataatttca acgattgtca aaagtattgt ttgcagctgt
      601 gttgccagtt tctatagata aatcacagct tttgaaacat aagttttgta ctgcttcgat
      661 tcgcccgaat tcttttgcag ttatttggca atgagaaaag cagacatctt ctataggctt
      721 ttcaggaaat gctactaaat caaaagctcg ggaaggaagt gagtaacctg gagaaaggca
      781 tgcctgtacg tttttgatgt gtatgtcttt gactttcgtc atttgctttg attcaggtat
      841 ttcttcagcc actgcttgcc aactttctgg catttgagca aggtcattgc tattttttct
      901 attgtattca ttatgccagt ccattatcca agaaaaagga aattgtacat caatcatatt
      961 cagattttct accctgatat tttcgatcac tccccctctt tcttttgaag atttcattcg
     1021 aataccacag tctgactgaa aaaaattgat atcgtgaata tatacgtcac taactccagc
     1081 actaacttcg ctaccaatag ttacaccgta tcctgaacgg attttacatc tagcaacctc
     1141 gattcttgat gattttcgat tgactcttgc accattgcca tctctgccag acttaatagc
     1201 aatacaatca tcaccacagg ccaaatcaca ttcatagaca cgcacatttg ttgaagaatc
     1261 aatatcaatc ccatctgtac ttggtccatc attgttgcgg atgattactt tttctactaa
     1321 tacatcgtta gaataagtga tttgcaagtt ccaaaaacct gatttctgaa aaatcaaatc
     1381 acgaacaaca acatgttctg catgatacag taaacaagcg cgtggacgtt taatagcata
     1441 atccgcaatc caacgaagtc ccttttgatc gtattcagct cttgtcccgc ttttttgatc
     1501 ttttccccaa tacagctccc accaatggtc tccttgaccg tcgattattc cttttccttc
     1561 tataagaata tctttcgccg agagaacatt caaaatcgct gctggccatt tcatttctac
     1621 gccggctact cgtgtatcga tttctggaaa tgcttcgata tctgttctcc ccttgaggac
     1681 tgcaccttct tcaaagataa gatgactctt atcttttaaa aaaagggctc cgactaaata
     1741 aatgcctttt ggaataaata caggtaagtg ttctttcgca ccttgatcga ttgctttttg
     1801 cagtattatt gtatcatctt gttgagtgtc gtttgtttta aagttcaaag cattgataat
     1861 cccttttttt gtccgcaata cgtttctccc tatctttcat tgattttttt caattcttca
     1921 ccaataaata cagtttttga acgattcagt gggtaaccta ctagcaaaat gattgcggat
     1981 acaaataagc agaatgctgg taacaagttt gccatcgtat aaatcctttc ttgtactaaa
     2041 actgattgga tggctccacc tgttgtagac gattggtaac caatgagtgc cagcataaat
     2101 ccgcctaccg ctcctgcaca tgcttgtcct acttttctcg caaaagaatt tactccatat
     2161 actgttccat cttctcttaa gcctgtctta tattgatgat aatcaatcac gtcagtaatg
     2221 aatgcccaga ccatcaaatt gaataaacca gatcctaaag tagcgataaa taaaagaatc
     2281 agatataccc atgcatttgt tatatgaagg aaatacatca gcaagtacat acatgaagaa
     2341 gcaagtaacg caaatacgct tgcttccttt ttaccaaata attttgtcag ctttgtagca
     2401 aatggtgcta aaactactac tgttccgtaa gtaaataaaa gagcaacaga cattgcttgt
     2461 ttattatgga agtaatcatt gaataaataa gtcatattcg tcccagctaa aatctgattg
     2521 atcacaatga aaatgtcaac aatgactaaa ataatcagtg ctcgattagc taacaaacct
     2581 tttaccaact gagaagcaga tactttttcg tttttgacaa ctcttactct ttcagtagtc
     2641 aatttacaag ttaacatata tgcgacaaat gctattgcag cacatccgat tgaaatccaa
     2701 aagaaatgat tccctgaaac gacttgttgt ccactcttgt tatgggtata cattaaaatc
     2761 ggaatgaaga atcctgtagt tgatccgcca atcgcagaac caatgcttcg gaacgtggac
     2821 aacgaggtgc ggtcattagg atttgagcta attgctgctg ccattgctcc atacgggata
     2881 ttgattgttg agagaaaaat cccataaaca atgtatgaaa caaaaatata agcaatcttc
     2941 accgtcattg gaagatgttg gacgaatggc aaaaacaaaa tgacagtcac taagcaaaat
     3001 ggatacttca tttttctgat ccaaggaata aatcggccag tttctgttaa ctctgagaca
     3061 tcacataatc ttccaaccat cacatcagca aacgcatcca aaattctcga aatcaagaat
     3121 aacgtaccta cgattgcccc tgaaacgcca agaacattcg tataataaat catcaagaaa
     3181 ctattgacta aacctaaaat aaaacagtta cctagatcac cgaacatgta gcctatctta
     3241 tcggaaatcc caaattcctt tttttctttt tttactgtat ttgctgtaac agcattttgt
     3301 gaaagcattt gaattcctcc tacctcttct agtaaacgtt ttcaaaatta taactctttt
     3361 tttcttgttt ttatattcac aatgttggct ttttattgca ttatcttgac agataaaaaa
     3421 caaaaaataa tctaaaaaca ttgatgcata agattgataa ctttttttac ataacaatat
     3481 atacaaacaa aaaaattcac taactagtga aaataagtta gtgaacctca tttattaaag
     3541 aattaaatta cagatgatct tttccatcat acttgaaaaa ctcaaaatca gcataacttc
     3601 ctcttttttt atttagatca tgcactgcta tcccgataaa atttccagta aaccctcctg
     3661 agagtataca catgtttttt tgcggaccta ctttcttcca ttcttcagtt gatacgtctt
     3721 tcaaatgaaa ctgtgctgtc tctttcttta cagtgacttt caaaaaagtc ttcttttctg
     3781 tgagtggaat cgtccaatct tctatttgtg ctactccgtt ttctgattct atcatacgta
     3841 ggcattttcc cttgtcctca ttccaagtaa cataacaata aatataattg gattcattta
     3901 agtacagtac cagtccagcc atctgattaa aattattagg catgtattca aatgctgtct
     3961 ccgcatcaaa agtaaaatcc gtttgccgaa tagctaacaa atgatgttcg aaggtagatt
     4021 gaagagattc accggaaatc agacgtaaaa aacctggtct acattttgta tcacaccaat
     4081 cagcatttgg caaaatgcgt ctggcattcc agttcttctt tagctctcct tcaaatgtat
     4141 ccataaactg atgatctttt tcttgttgta cctcttcatt tgtctcaatc attatttctt
     4201 ccatcggagt tgtctggcta tctttaagtc ttaaccaatc gtcggacgtc caatacactt
     4261 cttgaatagc ggtttctctt cccaaaatag ccgcaccgtt taatggtctg gtacaaagat
     4321 atgtcatgta ccatcttcct gacggggttt gtactaagct cccatgtcct gtacattgca
     4381 atgtagaagc aggtttgtca ctagctgtca gcataggaaa accaggatca agctcgtaag
     4441 gaccagtaat attctttgct cggcaaactg taactgaatg tcctcttcct gtaccgcctt
     4501 ctgccgtcat caaataataa tacccgttac gaaaatacaa atgtggtgcc tccgttttcg
     4561 ctaattctgt acctgcaaat attttataga tgggaccatc aagcttcttc ttctctgcat
     4621 catattcttg taatacgata ccgctggatt tattcggtgt gtccagacga taatcccata
     4681 gttcattgac aagccatttt tttcccgttt caagatcatg gaacaaagaa ggatcaaagc
     4741 cgttactatt taaataaatt ggttcggacc aaggacctgt gatctcagat gcagtgatca
     4801 aataattgtg gacatcttta aacggacggc ctgcactttt gacatctgtg taaacaagat
     4861 aaaacaatcc atcagcataa ctgatctgtg gcgcccatat acttccattt gtaggatttc
     4921 cttgcaaaga tacttgagtg gtcaaaatat ccgttgtatg tgaccagttg actaaatttt
     4981 tcgactcata tactcggatt cctggaagcc attcaaaaga ggagacaata atgtaatacc
     5041 gctctttcac tcttaatata ttaggatctg gattgaatcc tcttaaaata ggatttttca
     5101 ctttgatagt ctgcatgatt caggctcctt ttctcattct tactcgatat aaactgaatg
     5161 ccccagctgc taaaaaagca agtgaaataa acaacacaac ggcaaacaga ccagtcatgt
     5221 cctgtaataa tccaaatgta ttttctaaag acaaagttga atgatctaca gcaagcgact
     5281 gataactttt tttaaactct gacgcagaca tattacttaa agtaactgct aacccgccaa
     5341 agaaaatcaa agatagactg ccaccgacca ttcctagcaa tagttcccat tttcttttca
     5401 tttctgaacc ccctgctttt ctaaatgtat ccattcaggt cttttgatcg atgtttcata
     5461 attgttatta cgttcagaga atttgacagt ctgttcattt ttcttgtcat cccaattatc
     5521 ccaaccagtt gggtgaacat gcccacctat cttacacgct tcaaataaga catttgcata
     5581 cggacgccac ggtcttccta aatagacgtt tctagtatca ggttctgcag tcaaggtaca
     5641 gctttcaaaa cgataccctg ttttttgatt tttcggcgta gatgctgctg tgatgtatcc
     5701 aggattttta ctggctaagc tatggatatg acactgatga aaaacggcat ctgccccacc
     5761 aaatatgaaa tccaccgttc cttcaatcca gcaatattca taggtctgac gacaatactc
     5821 aggattggca gaagccggtg tacaaaaagg agtaccatct ttttgcaaat caggcaacgg
     5881 acccgtgcat aaggtatcct gataaccaga tagtcgacag ttttttaaag atacgcgatc
     5941 tcctgaattg aataaagcaa cagcctgtcc taccttttct cctggacctg ctgtattttc
     6001 gatgtgaaga ttttctaata cgacattcga tccttctaag aaaaaagtag cacttcggaa
     6061 agtccctaac tggctaccgt caagatactc ctgttttgca aaacgattac cggagataac
     6121 tactgttcct gctcccacaa gtttaaaatt ggataatctg ctttctatat attcatcata
     6181 tcttccttct tttactatca agacctttgg atggctatcc ggcaggcctg ccaaataatc
     6241 gattccttct tggatagttt caaaatccgt cggggtatct atagcattct ttccaattat
     6301 cagtttgtct ttcatcctca taaatgctcc cttatgaatt gtttccaaat atttctcatt
     6361 tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag aaaagttttc tggactaccc
     6421 attgattgat actttttgtc cagccctttt ctcagcttca gtacgccttc gtccaagcac
     6481 atcgtatcgt tttttccaac taagctaaga cgagggcgcg gagcgatttt ttcttggatt
     6541 gctaacgttg tataagctgt caatagattt ggcacatagt aataaaaccc atgatgatct
     6601 aggcggcgat gtagcaaaag tgtttcgatg tccacctgtg ccgctagatc aatacaaact
     6661 ttgacacgag aatccaatgc tgtcacccac cagcttaata agcctcccat agaaagacca
     6721 atcgttgcga gccgttgagt atctgtgtct tcccttgtct ccaaataatc gatcaagctg
     6781 atcacatcat aaatacgcat tccccataac gtttttcctg ttaataaaaa ttgtttgaac
     6841 aactcgcttt cagaaatacc tccgcgttct tcaaatcccc aagcatcaat cgaccaaaca
     6901 gcatatccca ttcctgtcag tacttctgca aaagatggag aagctaagta aggagcacca
     6961 tggatcaatt cacttttccc gactgaaaaa tctccgccgt gagaatgcag gtaaaggacc
     7021 gtcggaaacg gtccttcttt ttctggataa gcatagaggg ctggtactgt ttccaattca
     7081 tttaagtaca attgatagta ttcaagcata aaaccgtttt gtttttctcg gtataatcgt
     7141 tttccactag gggtatgtct gctagatgga gcacctaaaa aatcattcaa ctgcataagc
     7201 aaagttttta cgaacaacca gttcatccat attttctgat tgatttgatt ggaatttctc
     7261 aatactgatt tctttagtat tttctaatag aaatgctggt ccctcgtgat tttcaatcga
     7321 catctggtta aaggaaacct tacccgcaaa gccaatataa aatccttgtc tgctcatttc
     7381 ctctattcct gtcatcatag caggaacacc gggaactgca tcttgggcca tcgagacatc
     7441 gatattgtca aatgtgattt cttgtatgaa ttgttccgct aatccataaa taaaacctgc
     7501 agaagcatgg acattccttg ccgtgatatt cgaaaaatgg atccttctga atgctgatgt
     7561 ccgttcatct atcggataag cctttttttc ccatacatac ggttctttcc ctcgcggacc
     7621 acagaaataa taaagattca gtataaatgg acacatcaca ttatccatga ctatattgga
     7681 tactcgaata tcttcaacga tcccgcctcg acctcgtcta gatttcaatc gtattcctcg
     7741 atcagtttct tggaagatac aatttgaaat ggtaatatta cgaatactac cactcatttc
     7801 acttcctaat acgacaccgc catgtccatg gaccatcgtg caatttgtga tcgtgatatt
     7861 ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc ttgattgcaa tacagtcatc
     7921 tcccacgtca atatggcaat tactaatacg cacatttttg catgattctg gatcaattcc
     7981 gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag ttatcaaacg ttgcattact
     8041 gcaaagaatc gggttgatcg tccaacttgg tgactggatc aatttaatat ctttcacagt
     8101 aattcgatga cagttatgga aactcatcaa tttaggacga ggataagcga gattatctgg
     8161 ttcatttcta aaagtatgcc accatttttt tccatttcca tctaatgtac cgaaaccagt
     8221 gacagagatg ttctctacat tttgagcata gatacaggaa gcataaactt ttcgatggac
     8281 tccttcccaa cgtgaatgaa caacaggata atcttttgga tcgtcagaga atttcagaac
     8341 cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa aataaagctc ctgttagaaa
     8401 ttcacctgct ggaacaacta cggtcccgcc tccgtcactt gctgcagcat caatggcttg
     8461 ttgtattgct tcagtattca attcatcaat tgatgcgcca aatgtcaaaa tatcatacat
     8521 atttataatg cctccacttc aatcaatgct tgcaagaatg caccaacgcc tttttgatca
     8581 ttacaaataa ttggttcact gatgtagtaa gcatatgaac catctcgctg atctgctccg
     8641 cctaaccctg ctacttgaca gtttttattc aagttcagcc agccttcttt tgtttccaga
     8701 acaaattcat caagtaatcc ttgatgtgat ttcttcactt gtggaagcca ttcttctttg
     8761 ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa caaacatact actgccggaa
     8821 gcttccagat aatttccttt ccgttttgtt tctgttgtta cttgatacca tgtcccgctg
     8881 ttttcttctt gatatggaag taatgcagcc agtgtctttt ctagaatttc tttcagcaaa
     8941 gaacaatctg tttcttcatg aagtaattca taggtatctg ctgcagccat caaataccag
     9001 ccaatcgatc ggctccaaaa atgatgggat agacctgttt ttggatcagc ccatggttgg
     9061 accttctttt catcccatgc atgaatcagc aaatttgttt ttggatcgaa tagatgttta
     9121 taactgattt caaactgtct caaaacatct tggtaatctt tttcttcact aaaattctgg
     9181 ataaattcag cataaaaagg agaacccata tataagccat ccaaccaaat ttgataagga
     9241 taaatttctt tatgccaaaa agctccttcg gatgtgcgtg gatggtttgc caactgctgg
     9301 gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg tttctttgta caataaaaag
     9361 aaaagctttc ctgtatttac atggtcgata ttgtattctt caacgctgta gccacggatc
     9421 gttccatctt cttgcacgaa atagtcgata ttgtctttta tgaaatcaaa ataaattttt
     9481 tcttgggttc gttcccaaag taattggaac cctttaagta cgacgccata gtcataagac
     9541 cattttcctt tataccactt ttcttcatat aagcgaggtg ttctttccat gatacttttt
     9601 gctaatttct ttgaccagtt ttccattatt tgctctcctt taaaaattaa aataggattt
     9661 gggacaaata ccaagaagta tttgtcccaa tcgtctatat tactgtgccg ctgctttgta
     9721 tgcttcctcg tactcttgtg tgattcgatc gcctccttgt gctttccagt cttctacagc
     9781 ttttttgaat ccatcttcgt caagctgtcc catgatgtat tgcaccgtag catcaatgac
     9841 gattttttca atttctgatc ctacttcatt gttcgtcggt gaatccaatg gcacagaagg
     9901 atccattaca gcaaattctt cattttcttt gatcaattga ttagagaatt ctttttcagg
     9961 gtctgtatct ttgaaagaga acgtcacttc actaggacga gagcttgata acggttgcac
    10021 ctcttgttgc caaagatcca tatcgataaa ttcgatagaa ccatcatcat tcaattcata
    10081 atgcgttcct tcgataccgc cagtcatcaa tttatacaca tcttcttcta aaagatcatc
    10141 aacaaattga agtaatttct tcaattgtgc ttcgtctttt acttgagatt tgggaaatgc
    10201 tagcaagccg ccaataccat ttccttctga ccaaacatga taaccgccgc cttcaccatc
    10261 tgagatcttg tttacaggca ctaattccat atcatcttga ataccttgac ccatcgtctt
    10321 cagattcttg atatccacca ttccagtgta gattccagtt cttccttgtg caaattgttc
    10381 ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc aaatagccat tctcaaatag
    10441 atctcttgaa aaattcaaag ctttcatata ctcttctgta tcaaattctg gagtgaattt
    10501 gccattatcg tctacagccc agccatttgg tgctccaaaa taagaagtga aaagtttaaa
    10561 gctggtataa cgaatatcac taggcgcacg atcgccaaaa ccaactgtgt cgtccacacc
    10621 atttccatca ggatcgtttt ctgtaaattg tttagctact tcataaagct catccatggt
    10681 agttggaaca tctagtccta gattatccaa ccagtctttt cgaatgacta agccagcacg
    10741 agcgtagtct ttttgtatcg gtacgccgta caaggcaccg tcgatactcg cagcttcgat
    10801 tctatcttca gaaatttgac ttaaattttt atagtctttt aaatattctg atacatccca
    10861 gaatacgcct gattttaacg cattgcggac agaagagttt ttcaacatcg caatttgaag
    10921 tgtaacgata tctcctagtt catcagaagc caatgcagtt gttaatcttt cttcttttga
    10981 agcatcagga atccagttga atgtcagttc cgtatcggtt ttttcttcga tcaagttcac
    11041 aatcgtgtct gtaggcggtg aagcatgatg caaaatattt aaccaagtga ttttagcttt
    11101 tccatcttct gtctctgaag aactgttact tgaactgctg ccgcatgcgg caagtgctag
    11161 aattcctgca aaactaagac ctaattttac gagcgttgct tttttcataa attttctcct
    11221 tcactgtttt aataatattt ttgttttctt ctgttttttc tgtatatgag ttcttccatt
    11281 ccaaatggca aaatatagaa tttgctgtcc acgtgactgc ggaaaagaag agactgccac
    11341 cgataaaggt taaaacacct ggcattgttt ggatgatcat cagataaatc cctaccatac
    11401 ttaatatcaa cactaatgag taatgaagat gtgagaatcc gaataaaaat gccattttca
    11461 ttttgaaaaa atatcctttc caatcataat gagccatgat tgggaaaata tagagtgctg
    11521 agagcacata tatgaacgca gcaatataca aagcgatcct aagataccaa ttgtgtatga
    11581 aagaaagatc cacaagtaaa acaatcccga tggcgccata tatccagctc atcagcaagg
    11641 tttcacgata gttttcttta aaatatgtga agtacgtttt cgtcaaagga aacgtttctt
    11701 ttgagcgcag ccattttcta atcactgcaa ccatcgcata cgatgcagga ccgaaagtaa
    11761 aaactcccaa acctgcgatg actaaaccgc cccacagaaa attcaaataa accaactgta
    11821 aaatacgatt tagccttccg tttattttat ctaatgactg cagcatatct ttccctctct
    11881 ttctatctta gtaaacgccg tcttctccta gctttttggc aatcttatta gctaaaatta
    11941 ccataaacaa tcctacgacc cctttaaaca atcccattgc tgtactgaaa cttaactgtc
    12001 cattcttcaa tccagctgta taaatatagg tgtcgaaaat ttctgcaacg cttcgattca
    12061 atgaattcaa taacaagtac atatgctcga accctaagtc taatgtctga ccgattttta
    12121 gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa tgtaacatgc catgttttgc
    12181 gtaagcgtcc tgctccatcc atttctgcag cttcatacaa ctgtgtatcg actgcagtta
    12241 tcgctgaaag gtagatgatc gtagaccagc ccaactcttt ccagatcact tgtccgatat
    12301 atactgtgcg tgtccattca ggggatgtta gaaaactgat tttttcaaat cccattgttg
    12361 caaggaattc attcaatacc cctccgtcaa cattcaaaaa tacataagta atcgaaacaa
    12421 tgattaccca agacatgaag tgagggatgt aaatgatcgt ttgaatcccg tttttcaatc
    12481 gtttgttcgt tacttcattt aatagtaaag acaaaatgat tggcatcgga aaaaacacga
    12541 agatatttaa tccgaacaag atcaacgtat ttcttaataa tgtaaagaat gtcggttctg
    12601 taaacaaacg aatgaaatgt ttgaaaccga cccaaggact tcccattatt cctaaaaatg
    12661 gttgataatc ttgaaaagca ataacgagtc cccccatagg aagataccta aaaatcaaga
    12721 aataaaggac tcccggcaga atcatcacat ataaaaattt attcgttttg attcctgcaa
    12781 ttattttttt tcgtcttctg agcttttctt cttttttcga tagcgtcagt tgaaatggtt
    12841 cttgtttctt tacactttcc ataagcccct ccttagtttt gtgaatgttt gctgcttatg
    12901 acttcatgat agataactat ctgaattacg tatataatca tttttatcgt aagtaaaaac
    12961 gcttacatag caatgatttt tgtgctatac aaaacgataa agtgtatttt ttcacaaata
    13021 aactacgcat agcaaacctt ctattttttt ctatctgaca cttagcatag gaaatgatta
    13081 tatacgtaat ctttcaccga tgataaattg aacatataga aacagaaaca tttaatttat
    13141 tttgaggagg agcctatcta tatgaaaaag aaagtctcta taggagaaag aagttttaca
    13201 cttttcaaca atacgtttct aattttgcta gctttgatct gtatcgttcc ttttttaaat
    13261 atcatcgcta catcttttgc ttctacacaa gaagtcgtcg cgaaaaagtt tatccttttt
    13321 cctactacct tttctttaga cgcgtatcgc tacattcttt ctacgccgac catttttaga
    13381 gcacttgctg tttcgattgg tgttacaggt gtcggaacga tcgtcagtat gtgtgcaact
    13441 tcactgatgg cctacggtct ttccagaaag tatttgtttg gtcgaggttt cgtcaacttt
    13501 cttgttgtat tttctatgtt gttcagcggg gggatgattc caacgttctt agttgttcgt
    13561 tctctagggc tggtcaattc ttactgggcc atgatcctgc ctgtggctgt taatgcaatg
    13621 aatatgatca ttatgagaaa ctttttccaa gccttgccgg atagtttgga agaatcagca
    13681 aaaatggatg gttgcacaga ttttggtgta tttttcaaaa ttatgctgcc attagcttta
    13741 ccgtctattg ctactatctc gcttttttat gctgttactt actggaacac ttatatgact
    13801 gctattttgt atatcaacga ttcatctaaa tggccgatac agattctttt acgccaaatc
    13861 gtcattgtat caagtgggat gcaagcagaa agtagtgccg ttgatgtgat tccaccagca
    13921 caaacagtta aaatggctgt tatcgttatt gcaacagtgc ctatgctgat tgtttatcct
    13981 tttgttcaga aatattttgt caaaggagca ctcgtaggtt ctgtcaaagg gtgattatgt
    14041 actgaacgca aaatggtagt tgtgtcagaa gtggtcagct tcaagaaata agaaggcatt
    14101 tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt atttttcgaa gaatctactc
    14161 ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca gccttgagcg atgataaaaa
    14221 ctctcacaaa cattgcgacg agtaacccac aggagcaata agaagcgatt ttgaaaaata
    14281 gcttcccata tttcacaaaa ttatgcctta ttgtcgatag cctgtctctt gaacgccatt
    14341 tattcgtaaa aaagaactgt gacaagactt ttgtcacagt tctttttgcg tgcttagcct
    14401 tgaaatttaa ctatccataa atacaaatac tagggttatt ctaacttctt ttattgcttg
    14461 aatcagtcca caagaaaaac agcctcttga ttaaacggcg tttatcttgt tgattctgcg
    14521 gcaataaaat cagccagcag caaaacatga agtgctgttt tttgctgtcc tcttttattg
    14581 cttgaatcag atcacaagaa aaacagcctc ttgattcaca aatatttttt tcgatattct
    14641 cccggcgtaa tcgattcctt ctttttaaag aaacgaataa aattttgcgg atttcgatat
    14701 tgcaatcttt ctgcaatctc ttttacggag aggttagtct ctttcaacca tttttttgct
    14761 acctctaaac gatgattttg aacaaaatca ccaaattttt ctcctgtttc ctttttaaat
    14821 acattactta aatagttagg gttataatgc aacctatcag cgatcacatc taaagacaga
    14881 tcacgatcat attctgtgta gacgatatgc ataatattct ccgacaaatt tttaaattca
    14941 tgatcagtga ttgcttgaga gcattctgtg attggcagaa ctaattgttc taaaacaagt
    15001 ttttctagtt ctttgggatg ataattgttc agtgctttta cataaatttg tttcatgtta
    15061 tcaaagtttt ttgaatctac tcccaataac tgtcctaatt gaacgagttc attgatcaag
    15121 cggaccgaaa ctacttcccg actcaaagga tttttatttt gtgcaaacaa tgcatcaatc
    15181 aattcatgta ctaaattctt cacttcctga tttccagaac gaatggcttc aaacaatcga
    15241 ttttgttctt ctatcggata tctaataagt gttttttcat gagcagcagg aacgatttcc
    15301 tgataaaaaa taattgaact tggaccagta tttacacgat aatacagggc ttcttttgcc
    15361 cgatcaaccg attgcttgct ttcattcagc gtttcgaacc ggttactgat gcctatactg
    15421 atagttaaat tcaagtattc ttttacacgt ttttgaatga gtcggcaata ctccatgact
    15481 tttttgcttg cttctgcatc atctttatcc attcgataaa tagtggcttg catttcttca
    15541 ttcaaaacga tcggcagcat tcgattttcc tttggcacaa tttccgtgat catgttgttg
    15601 atcgctaaaa gtagcaaatc acgctcccca ccgtgactat cttctaacag atcgatttga
    15661 accaaagctg tataatagca gtcattttga gaactataac caaattgctg caaccgctga
    15721 tctagttctc gcctttctac tcgatttcta aataaactaa gtacaaatag cgtttccagc
    15781 tgtggctttt gggtaactaa gtgtgcactt aacgcttctt tttctcctat gatccgactg
    15841 actgattgag cgaccaaatc tagttctttc ccactgaatc cttttgattt aagattcaga
    15901 cgttcctgta tttgagaaat cggacgggca aatctgtccg aaaataagta ggaaaaaaga
    15961 ccaatgagtc ctaccagtgt taaagacact gcaactagcc cgattctcag attgcggatc
    16021 attgcgccaa ttgcactaga gtcaacttct aatctgtaaa tccaattatt ataatctgat
    16081 ttcagtacta tatacgtttt atctttttct cttatcactt tcagctgatt gtaatctaga
    16141 tccacgtccg ttaatacagt tggttgctct ttatttttta ctgtctgagt agtaaaaagg
    16201 ttgccaactt cgttttcaat agaaagtatg tcttctccac tttcaccaac aacttgttct
    16261 attgtatgtt tctctatcgt agcaattccc aatgcatatt tttctttttc aaacattggc
    16321 aaagaaatca ccatttcgat tcctttgttt ttcttttgcc aaaataaatt atttttgtta
    16381 tttatgtact tctgttgata cttttcaact tcctcttcgg tcagttgact caacgatcca
    16441 ttgattactc cccatttacc agccaaactg actaaatcat aattattatc atccataaca
    16501 atcgtttcta taaaatttag ttctttcctg atttcagaat aagtggagta atctgtatat
    16561 gtcagcggat gcttgaatat gtccgagaaa tttttggtcg aaccatacgt agaaaacgaa
    16621 tattcaatcg ttctaatttt ccattcaacg ttagttttaa cttgcctgag caaattcttt
    16681 ttgtctgtta aataactcga ttcgatcgat tcataggtat tctggtaaat gaataaacta
    16741 aatcctatga caagtacggc tcctaataaa aacatcggta aaaatatttt ataaaatact
    16801 gtacttttct tcattttctt cctctcctcc ttttaaaata aaaggctttc atttttctgt
    16861 aaaattgata ctcatctaat taaatgagca ttccccgctt tcaagaatag cataattaaa
    16921 aataaaaatc caagtaattt ttaacgtgtg caatcttttc atatcaaaga tctattgact
    16981 tatcgtagtt atcgtaatat gatgaatgcg ttaacaaaaa gtgaaaaagt aaggaaaatg
    17041 atggaatggt aacgatcaaa gatatcgcta aagcagctgg cgtttctcat acgacagttt
    17101 ctcgtgcttt gaataacagc agtttaataa aaaatgaaac aaaacaaaaa atacgacaac
    17161 tagctaagga aatgaattat tcgccaaact atagtgcaaa aggtttagtg aatcaaaaaa
    17221 aatatctaat cggactattt ttctcaagtt tgcataaagg aacttcatca aatttcctag
    17281 cagattccat tgcaggcatc cattcagtat tagacacaaa ttacagttta tctgttgaaa
    17341 gtatcgatgt tgttgattta acagaaatca acctgcaacg ctacgatgga ctgatcgtta
    17401 tgagtcaaag tgatgatgat cagcctttca tcgactatct caaagaaaaa gaatttcctt
    17461 tcgttgttgt caatcgtcac atcaatgatc cagcggttat caacgtcact gccgatgatg
    17521 cggccggtgt tatgcaggca gtagattacg gtattggatg cggacatacg aagatcgctt
    17581 atattggcgg aaagaaaaat tttcgttcga caaaagaacg ggaaaaaggg gtattgaaaa
    17641 gtctaaaaaa agcgagcatc cctgtcaatc tagcctattt tttaacagga gattacagct
    17701 tagaaagcgg cctccaaaac atggaatacc tattatcact ttctgacctg cctactctgg
    17761 ttttttgcgg caacgacgac atagcgattg gtgcattgag agctgtacat ttagctggcc
    17821 tccgtgttcc ccagaacatt tctctgatcg ggttcgatga ttctccagtc gtctcctatc
    17881 tcaatccgcc gcttaccacc gtacgtaagc ctttgaaaga aatgtgtcaa gaaggaacaa
    17941 agcttttact agatttgatc gaaggcagga cgatcgaaca atctaaaatt gagctctcca
    18001 ccttgttgat ccttcgtgag tcgatcaatt acttgtaaac tacaagtaat tttatataaa
    18061 aatgttaacg tgtgcataac acagaaagga agtttatatg aaaaaactga caaaagaatt
    18121 agtgaaaaaa aacgaagcac ctgtaaaagt tcttcagttt ggtgaaggca attttatgcg
    18181 tggatttatt gattggcaga ttcagcagct gaataaccaa caattgttta aaggtaatgt
    18241 tgcaatcatt caacctttag aacaaggtct tggaaatatg atgaaccagc aagataattt
    18301 atatacggtc attttacaag gactcagcaa tggcgaaatc gttgatactt ccgagattat
    18361 caccgtagta gaacaaatga tcaatcctta tgatgaatgg gatgcttatc tatctctagc
    18421 agaaaatgac gacttggcct atatcgtctc caatacaaca gaggcaggaa tccaattcgt
    18481 ggagactgat aatttaacag atactccccc acagagcttc ccgggcaaac tgacagcctt
    18541 tttataccga cgtttcaaat taaacaaagc tggctttacc atcattcctt gcgaactgat
    18601 tgacagaaac ggcgaacgtc taaaagaaat cattttacgc tacgcagaaa aatgggattt
    18661 agaacctgct tttgttgagt ggttagaaaa agaaaatgtt ttctgttgta gtttagttga
    18721 tcgcatcgtc ccaggttatc ctcgcgaaga tgcaggtgtt ttcaatcaac ggcataacta
    18781 tgaagatcaa ttgatggtca aagcagaacc attcatgctt tgggtgatcg aagggcctaa
    18841 agaattggag aatcaactac cattcaaaca ggctggcttg aatgtcatca tcacagatga
    18901 tatgactcct tatcgtgaac gtaaggtgca tttgcttaat ggaccgcata cagcgatggt
    18961 ccctcttgct ttgttggcgg aattggaaac tgtagaagaa gtaatgaaag atcctttatt
    19021 ctccgcatat gttgatcagt tattcaatct agaattgatt cctatgcttt ctttacctaa
    19081 agatgaatta gccatttatg ctgaccaaat caaagaacgt ttcttgaatc cttttgccca
    19141 tcacaaatta gaagcgattt cattaaatag tgtttcaaag ttcagcaccc gtctgcttcc
    19201 agtgttcaag aagtatatcg aagaacaaaa tcaggtacca ccgttaatca ctgtttcact
    19261 agctgcgcta cttcttatgt atcgaggcga tcaagtaaag ccacatgatg atgaaaagac
    19321 gataagcgaa tttactgatg cttggtctga taatggaaca gctattcctc gattattgca
    19381 aaacgctgcg ctttggggag aagatttgtc tcaaataccg aatgtgacag atacagttca
    19441 agaaatagct gcaaagatcg atcaaaaagg tgtccggtca gtattacaag aaatgaaagg
    19501 aacgatggtc catgcttgaa accctgcaac caacaatgaa attaaatcga cgagacagtg
    19561 tggtcgtcgc attaacccct attccaaaac aaacacaact gacgattgat cagcaaacga
    19621 tcaccactct tgaagatatc ccgcaaggtc ataagattgc attgggtgat ttgaaagctg
    19681 gagacaacgt gatcaaatat ggctatccaa tcggtcatgt gacgacagac gtaactgccg
    19741 gtcaatggct gcacactcat aatgtaaaaa ccaatctttc tggtgaattg gattatcgat
    19801 atgaaaaaga tgtccatcca agtcattacc tttttgagaa ccgaacattt caaggttact
    19861 tgcgaaaaaa tggaaaagta gggattcgta atgatttgtt tttagtacct accgttgggt
    19921 gtgtgaatgg cattgctgaa ctgatcgtca aacagttcaa ggaaaaacat ccagatctgg
    19981 gacagtttga ccatattacg attttaaagc atccttatgg ctgttcgcag cttggaaaag
    20041 atcatcaaaa tactcgagaa attttagcgg acgcagtaaa tcatcccaat gccggaggag
    20101 ttttagtatt tggattaggt tgtgagaaca atacagttcc tgaattcaaa aaaattttag
    20161 gtgaatatga tgaagaacga gtgaaatttc tagtggctca agatgtttac gatgagatcg
    20221 aacaaggtgt ggctctatta gaagagcttt taacagcggc ggaaaatgac cagcgcatat
    20281 cagtcccgct ctctaagttg aatgtcggtt tgaaatgcgg cggttctgat ggtctatcgg
    20341 gaataactgc taatccttta cttggtgcat tttctgacta cttgattgct caaggaggca
    20401 gcacgatctt aacagaagtg ccagaaatgt ttggcgcaga acaagtattg atggcacgag
    20461 cggaaaatga agaagttttt gactcaatcg ttgacttgat caatgacttc aaacagtatt
    20521 tcttgtctta cggggaacca gtttacgaaa acccatctcc tggtaataaa gcaggtggga
    20581 tcactacatt agaagataag tctttaggct gcacgcaaaa atcaggaagc tctcctgtcg
    20641 ttgatgtgct acaatatggc gaaaaaatca gaaaaccagg gcttagttta ttgcaagctc
    20701 ccgggaatga tcttgtagct gcctctgctc ttgcttcatc cgattgccag ttggtcttgt
    20761 ttaccacagg aagagggact ccgtttggct catatgtccc aacggtcaag gtatctacga
    20821 atacaactct ttttgaacgc aaaggtcatt ggatggattt taatgcaggc gtcttattag
    20881 aacaaccaat ggaagtaatc ttagaatcgt ttgttcaaaa aatcatcgct gtagctagtg
    20941 gagaagaaac gaaaaatgaa caaaatgacg ttcgagaaat tgccattttt aaaaacggag
    21001 taacactttg attttcataa aacagtataa aaagaaagaa ggaagattta tgtttttatc
    21061 tgatgatttt ttattaaaag acgattgggc gaaaaagctt taccattcct acgcaaaaaa
    21121 catgcccatt attgattatc actgtcactt agatccaaaa gaaatttacg aaaacaacaa
    21181 tttcagcaat ctgacagaag cttggttagg aggcgatcat tacaaatggc gcttgatgcg
    21241 agcttgcggc gtgcctgaag aaaaaatcac aggaaatgct tctgattttg aaaaattttt
    21301 agcttggtgt caaaccgtac caaaaattat cggaaatccg ctttacagtt ggtcacatct
    21361 tgaattgaag cgtttcttcg gtattgactt gttgataaat gaagaaaatg cagagaaaat
    21421 ttggacacaa gcaaatgaaa ttttggcagc tcctgatttt cgccgtcggg aaatcgcaaa
    21481 aaaatcgaat gtagaagtga tttgtacaac agatgatccc gcagacgact tgcactatca
    21541 tcagttgctt gctcatgaag aaccagattt gaaggttcta ccttcattcc gtccagataa
    21601 agcactcaat attgaaaaag aaggatttaa tgcatgggtc acactactgg aagaagcagc
    21661 tgaccaaacg atcaccacct atcaagaact gatagaagtc ttagggcagc ggatagaata
    21721 cttccatcaa catggatgtc gtgtttccga ccacgcttta gacatcttac gctataaaga
    21781 agcggatgaa acagtattag aagagatttt tcaaaaagca cgagctaaca aactattatc
    21841 ttcagatgaa atcgatgctt atcttacaga aacactgata catttgaccc acttctatca
    21901 ttctcataat tggacgatgc agctgcacat ccatgcttat cgtaactgta atacacaaat
    21961 gttggaaaaa ttagggcccg ataccggcta tgatggaatg aatgatcttt ctttgactat
    22021 tcctctgcaa aaattattga accgtgcaga agaaacggat cagttgccaa agacgatcct
    22081 ttattcattg aatccgaatg actatcctgc gattttagct ttgatgggct gtttccaaaa
    22141 agaacagaat ggaaaactgc agttaggttc tggatggtgg tacaacgata ctcgagctgg
    22201 tatgcgcgat caattgacac aatttgctga tggtagtgct ttggggaatt ttgtcggcat
    22261 gctgacagat tcaagaagtt tcctgtccta cactagacat gaatatttcc gtcgtgttct
    22321 ttgtgaattt ttaggcgaaa tggtcatgcg aggagaagca cctgaagatg aacaactttt
    22381 aggaagtcta gttcaagcga ttagttatac aaatgctaaa aattactttg gcttttttgc
    22441 ctaattgatt aatagtaaca gtaacgaatc aaaaacagga tagtgaaata acggaatccg
    22501 tttatccacg ttttagagaa gaagacggac caaat
//
LOCUS       DAJSBN010000042        21988 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJSBN010000042 DAJSBN010000000
VERSION     DAJSBN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.042"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..395
                     /locus_tag="ONT86_001894"
     CDS             <1..395
                     /locus_tag="ONT86_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352662.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ONT86_001895"
     CDS             398..1147
                     /locus_tag="ONT86_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2352663.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ONT86_001896"
     CDS             1159..1428
                     /locus_tag="ONT86_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352664.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ONT86_001897"
     CDS             1433..1597
                     /locus_tag="ONT86_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352665.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ONT86_001898"
     CDS             1590..1892
                     /locus_tag="ONT86_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352666.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ONT86_001899"
     CDS             1889..2050
                     /locus_tag="ONT86_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR2352667.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ONT86_001900"
     CDS             2047..2352
                     /locus_tag="ONT86_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352668.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ONT86_001901"
     CDS             2352..2708
                     /locus_tag="ONT86_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCR2352669.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="ONT86_001902"
     CDS             2695..2913
                     /locus_tag="ONT86_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352670.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ONT86_001903"
     CDS             2910..3329
                     /locus_tag="ONT86_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HCR2352671.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ONT86_001904"
     CDS             3326..3883
                     /locus_tag="ONT86_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCR2352672.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ONT86_001905"
     CDS             3880..4176
                     /locus_tag="ONT86_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352673.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ONT86_001906"
     CDS             4253..4666
                     /locus_tag="ONT86_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR2352674.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="ONT86_001907"
     CDS             5012..5386
                     /locus_tag="ONT86_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352675.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="ONT86_001908"
     CDS             5423..5590
                     /locus_tag="ONT86_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352676.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="ONT86_001909"
     CDS             5616..5960
                     /locus_tag="ONT86_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR2352677.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="ONT86_001910"
     CDS             5965..6246
                     /locus_tag="ONT86_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352678.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="ONT86_001911"
     CDS             6349..6663
                     /locus_tag="ONT86_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR2352679.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="ONT86_001912"
     CDS             6641..8335
                     /locus_tag="ONT86_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR2352680.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="ONT86_001913"
     CDS             8355..9533
                     /locus_tag="ONT86_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR2352681.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="ONT86_001914"
     CDS             9496..10182
                     /locus_tag="ONT86_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR2352682.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="ONT86_001915"
     CDS             10182..11342
                     /locus_tag="ONT86_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR2352683.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="ONT86_001916"
     CDS             11352..12227
                     /locus_tag="ONT86_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352684.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="ONT86_001917"
     CDS             12224..12535
                     /locus_tag="ONT86_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352685.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="ONT86_001918"
     CDS             12525..12878
                     /locus_tag="ONT86_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR2352686.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="ONT86_001919"
     CDS             12868..13269
                     /locus_tag="ONT86_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352687.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="ONT86_001920"
     CDS             13262..13666
                     /locus_tag="ONT86_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352688.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="ONT86_001921"
     CDS             13678..14286
                     /locus_tag="ONT86_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2352689.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="ONT86_001922"
     CDS             14306..14668
                     /locus_tag="ONT86_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352690.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="ONT86_001923"
     CDS             14671..14853
                     /locus_tag="ONT86_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352691.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="ONT86_001924"
     CDS             14870..18289
                     /locus_tag="ONT86_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR2352692.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="ONT86_001925"
     CDS             18340..19077
                     /locus_tag="ONT86_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2352693.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="ONT86_001926"
     CDS             19087..21378
                     /locus_tag="ONT86_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2352694.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="ONT86_001927"
     CDS             21402..>21988
                     /locus_tag="ONT86_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352695.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3246 c         4705 g         6104 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatctg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAJSBN010000043        21526 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJSBN010000043 DAJSBN010000000
VERSION     DAJSBN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.043"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            40..300
                     /locus_tag="ONT86_001928"
     CDS             40..300
                     /locus_tag="ONT86_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352696.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            341..568
                     /locus_tag="ONT86_001929"
     CDS             341..568
                     /locus_tag="ONT86_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352697.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            633..1205
                     /locus_tag="ONT86_001930"
     CDS             633..1205
                     /locus_tag="ONT86_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352698.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1206..1517
                     /locus_tag="ONT86_001931"
     CDS             1206..1517
                     /locus_tag="ONT86_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR2352699.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            1508..1768
                     /locus_tag="ONT86_001932"
     CDS             1508..1768
                     /locus_tag="ONT86_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352700.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            1929..2288
                     /locus_tag="ONT86_001933"
     CDS             1929..2288
                     /locus_tag="ONT86_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352701.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2285..3928
                     /locus_tag="ONT86_001934"
     CDS             2285..3928
                     /locus_tag="ONT86_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR2352702.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            3963..5045
                     /locus_tag="ONT86_001935"
     CDS             3963..5045
                     /locus_tag="ONT86_001935"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352703.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5089..6261
                     /locus_tag="ONT86_001936"
     CDS             5089..6261
                     /locus_tag="ONT86_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR2352704.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6248..6952
                     /locus_tag="ONT86_001937"
     CDS             6248..6952
                     /locus_tag="ONT86_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR2352705.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            6957..8117
                     /locus_tag="ONT86_001938"
     CDS             6957..8117
                     /locus_tag="ONT86_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR2352706.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8193..8471
                     /locus_tag="ONT86_001939"
     CDS             8193..8471
                     /locus_tag="ONT86_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR2352707.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8452..8823
                     /locus_tag="ONT86_001940"
     CDS             8452..8823
                     /locus_tag="ONT86_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCR2352708.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            8839..9243
                     /locus_tag="ONT86_001941"
     CDS             8839..9243
                     /locus_tag="ONT86_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352709.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9240..9650
                     /locus_tag="ONT86_001942"
     CDS             9240..9650
                     /locus_tag="ONT86_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352710.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            9711..10298
                     /locus_tag="ONT86_001943"
     CDS             9711..10298
                     /locus_tag="ONT86_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2352711.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10371..10838
                     /locus_tag="ONT86_001944"
     CDS             10371..10838
                     /locus_tag="ONT86_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCR2352712.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            10884..11192
                     /locus_tag="ONT86_001945"
     CDS             10884..11192
                     /locus_tag="ONT86_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352713.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11387..18121
                     /locus_tag="ONT86_001946"
     CDS             11387..18121
                     /locus_tag="ONT86_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR2352714.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18125..18997
                     /locus_tag="ONT86_001947"
     CDS             18125..18997
                     /locus_tag="ONT86_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HCR2352715.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            18994..20043
                     /locus_tag="ONT86_001948"
     CDS             18994..20043
                     /locus_tag="ONT86_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352716.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20047..20871
                     /locus_tag="ONT86_001949"
     CDS             20047..20871
                     /locus_tag="ONT86_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352717.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            20889..>21526
                     /locus_tag="ONT86_001950"
     CDS             20889..>21526
                     /locus_tag="ONT86_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HCR2352718.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7736 a         3219 c         4559 g         6012 t
ORIGIN      
        1 atacatcaac agctggttat gcgcttgcgg aggtacaaaa tgaatcatga gaagtttatc
       61 gaaaaatgca aggctattgt acgtgaaaga attgaaaatg agattgctga cctaagtgga
      121 gcagtgcctg agtttagtat tttcatagtc tggtcatgca aaacactgca aaatagcaaa
      181 gcattagtca gcgctagctt aaaaggagca ccgtattttg aaattacgtt gaacggggac
      241 aaaggtgaaa tctatgtaga tacttatctc aaaaaatcaa atgaatgtat caaagtctag
      301 caggtgctag gctttttctt tacataaagg aggctgcata atgagaaact actggtatgt
      361 atcactaact aatgaatatc ctcgaaccat tggtgattgt tcagtgcgtg ttgtgcgttc
      421 tgtacaaatc aaagggaagt actctattgt cgaaatgcta agagaagcta caccaaacga
      481 agtggataaa tgcaagctga tatattgcgg tcatggctat tggaaagacg agtatatcca
      541 atacaacatt gagaggtaca tcaaatgaat gataactatg attacatcaa gttgttagca
      601 agtaattatt agatgttttt aggaggaaaa ctatgaaatt aaaagattat atcagagaag
      661 ggtataacgt tgtaactgca cccaatctag cttacaaaat tcaagaagat tatcccaatg
      721 ctttagtgtt tactgataga gctttagatg ccattcctat agggaagtta ttagtagatc
      781 gtttttatag caataatcca gctgttctta gtgctaagcc acttcaaaac gcttatgcta
      841 ttgaacgctt tacttgcgag tttactggat atgagacggt agtaccaatg acaggtgaga
      901 caggaagaaa agtaattcaa cagatcaaaa agaggattgc tgaattgacg ttagatgatg
      961 gaagtaatac gaaccttcta gaaatcaaac taagagatac tgactcagta ccagaagttt
     1021 attataaagg tcaacgaatg gatgagatgc cgataggtct tgtcgatatt acttatcatt
     1081 gggatacgga agaatatgaa tgccaagtac caaaaggagc taacgacatc actattgaat
     1141 attatgattc acataataac aagtacttag atagaaaaat cattgggcac aagagagata
     1201 tgtaaatgat tgatgtttca agtaaacaag caagagctag gttctatgga tcaactgagt
     1261 ggaggaaact tagaagtcaa tgcttagagc gagatcatta tgagtgccaa tggtgtaaag
     1321 cagagggtag actaacaact caacatgatt caattcttga agtggatcat ataaaggagc
     1381 tggagtatca tccggattta gctctagata taaacaacct aagaacctta tgcaaagatt
     1441 gccataacaa aaggcacaac cgtatgaaat atcgtggcca acaaaagaaa agaaagtggg
     1501 atgatgaatg gtggtaagaa aaacagcaaa cagagatgat gtcaaacgag cagaagcaat
     1561 cgctatggtt gataaggcta ttgataagtt ggttctattt aagagtcagc tcattgaagg
     1621 aacaacaact gtagaggata tatctatcaa caatagacta ataggtaacg ctaatgaagt
     1681 aacagggaca tttaatatta atgtagacat tgattatacc ttgagtccat ttgcacatat
     1741 tgtagaaagc aacgagcaag acggctgata aatcagtata aagcgaagat atatagagtg
     1801 aaataccccc ggtcaaaaga ttttgagaag aaataaaatt tttgggaacc ggtggatggg
     1861 gtcaactctg caaataaatg atgctatttt cacacgaccc cctccccacg tagaaaggaa
     1921 gtgagttgat ggctgatctg caagagagaa ataggcttgt ttctgctgaa gaaaggcgtc
     1981 taaataaatt gtttaaggat attgataagg ataaaaagaa ggtcgtttct ggtttgatta
     2041 cgcaagcggc acgcttaaaa attcttcttg atgaaatgtg gattgatatt tccgaaaaag
     2101 gtgattatga gcttttctct cagtcagaaa atcaaaaacc ttacgagcgg gaacgaccag
     2161 ttgctaaaca gtataattcg agagaccaag catatcaacg tgtaattaag caacttactg
     2221 actatttgcc agaagaaaag cgcgaaccta caaaaaatgc agcgctagat ggtagtgatt
     2281 tgctgtgaca acattaaagc cattctattt tgatgaatac gtagatttat acgaaaaagg
     2341 ggtaatccct tttaataaag aaagaattca gttaattact tatttaaaaa acgaagtgct
     2401 agttcgggat gatatctatt ttgatgaaga aatgattcaa aagtttatca agtacactga
     2461 aaaaaatttt tttcctttag ctaaatatca aaaatttatc acacctttca ttttctgcta
     2521 tcaaaaagag gacgacgagg tgttttttga tgaaattcta aatagtattg cgcgaggcgg
     2581 cgggaaaaat gggttcatgt cagcaagaga ctcatttttt attagccctc tctacggtgt
     2641 cagaaattat gatgtaacga ttacagcgaa ttctgagaag cagggaaagg tatcttttaa
     2701 agaagtttat gaaatggtac aaatgcgcaa actggaaaaa caattttatt tgactaagat
     2761 ggctatagtc aacaatgtaa ctaattcgat ttttagttat aggacaaaca atcctaagac
     2821 tatggatagt gcaagagatg gctgcttaga atttgatgaa atccatcagt ttgaatcttc
     2881 agacctagtt gatattcagc gtagtgggct agggaaaata aaacatcctc gaacttttta
     2941 taatggtaca aatggtcatg ttagagaagg tttttatgac aaaatgttgg aacgagcaca
     3001 aaagattttt agtggagaga acaagaatga tcgcttgttt ccctttattt gtaaactgga
     3061 taacattgat gaaatggata atcctaaaat gtggtcaaag gctaatccaa tgttcgaaga
     3121 agattcagca tattcaaaac gtctgttttc tacagtgatg aaagaatata aaaaactaga
     3181 agatgagcca tcaggtaggc gtgaatttgt tgttaaacga atgaatttta cagaaggtga
     3241 tgcagaatca gatattacga ctcatgaaaa attaatggct actaaacaag aattgccaaa
     3301 tttgaatggt cgttcttgtg tggctggatt tgattatgca agtattcgtg attttgcaac
     3361 agtgggtttg ctttttaaaa ttgatgataa atttatctgg cagcaacata gttttgctag
     3421 gaaaaagttt ctagatacat tcaaactgaa agcgcctatt aaggattggg agaaaaaagg
     3481 tttagtgaca atcgttgatg agccatcaat tgatcctcgt catttaattg attggttaaa
     3541 tgaaaaaaga aaaatccatc aaatagaaat cgtttgtgcc gacggatttc gtatggattt
     3601 gctgaaacct cttctagaga aagaatcata taattttgag tttatcagaa atataagagg
     3661 tgtgcagtca aaagttgctc ctattattga agatggtttt gcaaatgaac ggtttatttt
     3721 tggtgatgat cctatgatgc gttggtatac aaataattcg tttatcaaag aagacaaatt
     3781 agggaataaa acatttttga aaaaagagcc agtaaggcgt aaaacagatg ggtttcatgc
     3841 ttttttggca gctttatata aacgagaaga aataactgat atggattatg aggaagcatt
     3901 tgatatgttg gatgagattg aattttaagg tatactcctt attgaaaggg gtagttatag
     3961 aagtgaaaaa gagatatttt ctatttgtta caatatttat aataatagca atttgtgttt
     4021 tgacagtatt tttaccacaa attactgagt atttaaatta tcggatattt accacaaaag
     4081 tagatgatag cgttactagt ttaagtaggc tagaatttat aaaaatatgt gcatctgtgt
     4141 ttggtcctat tttgactgta atggtatttg tgaatacttt aaatatgcag aaaaaaacag
     4201 agcaaaagca aaatgagctt gaagtaaagc aagctaaggc tgagaacgag agagcaaaaa
     4261 aagagctgtt aacacagata gatcgtgagt tctatttttt attaaatatg tttattgaaa
     4321 tacaaaaaga tcaaaatgcc aaacaagctg ttattgcttt acagaagttg gcaattttta
     4381 acgaaaaggg ttttaatcaa gactatgcat ttacttctaa agatttatca gatagtgaaa
     4441 ttgggagtac aattgaacaa tcaggaccgc tgaagggcac agttgaatat ataaaatttg
     4501 aaacatttga tgtaagcaag tcgatgggga attatcaaac aaaaacaaaa gacgttgcta
     4561 caagtttaca cgatcagaaa gtagctatat tgaatgaaca atatgaaaag atgcaaatat
     4621 atcttggacg gtacttcaaa atgtttcacc gaatagttaa aacattaaat gagtattatg
     4681 acgattataa tgattttgat gtgaagaggt atacaaaata tatagggaca ttaagaacac
     4741 aaatttctcc agctgaattc caagtgattt tattcaattc tctatatata aaacgaggtt
     4801 ttggcttagg aattcaactt attggaagtg gtttttttgg agatgatttt gattttgaaa
     4861 ctaatcaaca ttttgaaaca agtatcaatg aacagtggtt tctatcacta tcaactgttg
     4921 acagtgataa ctctataaag cgacaaaaat tatctgaata tattaaagaa ttaggatccg
     4981 tagaatataa aaaaatagct aattttgaaa gtttatataa actctttaat gaaactaagc
     5041 tctagaaata gggctttttt atttttcttt gaaaggtggt gaaaacatgt gagcttattt
     5101 gatatcttta agcagtcaat aagaaatgaa gagccttctg agtggatacc ggattttgta
     5161 tatggagagg aagtatctgc tcgggcatac ctcaaaatta tggcaaaaaa tactgtttta
     5221 gattttgttg caagaactat gtcaacgtta gaagttaagt ttaaaaataa agatgggaca
     5281 tcagactggg attatattct aaatgttcga cctaattctg atatgtcagc agctacattt
     5341 tggcaaaaat ttttttatcg gctcttagat gacaacgaag tgttagtaat atttacagat
     5401 gataatcaat tgctgattgc tgatgatttt agtcgaacgg agtatgccgt gtatgatgat
     5461 gtttttacaa atgtgatggt aaaaaatttt gtctttgaac gttcatggaa catgtctgac
     5521 gtcatttata tggaatataa taacgaagaa ttagaaaggt ttacacgagg tttatttgaa
     5581 gattacgccg cgttgttcgg acggattcta gaagttgcaa tgcgtaacaa ccaaattcgt
     5641 gggtctgtat ccattgattc aacgggaacg gcgaatgatg aaaaaggaaa agatggtaag
     5701 actagatctc aaagattaca agaatatatt gataggattt ggaactcttt taaaactaac
     5761 tcagttgcaa ttgttcccaa agtgaaaggc tttgagtatg aggaatacac caataaacaa
     5821 ggcgtatcga atcaatcgct agatgaactg aataagatga agtcgtcttt aattgatgat
     5881 gttgccaacg ccataggagt acctacggcg cttatttatg gtgaaaaagc tgaactggat
     5941 tcaaatatta aagcttttcg taaattgtgt attgctccac tagttaaaaa acttcaagac
     6001 gaattggtgg caaaaataat tagtcgtcaa gagtacaaaa acggggaacg tatagaagtt
     6061 aaaaaagttc tccaaaaaga tctattagag tatgctactc aaattgataa aataacatct
     6121 ggtggtgcgt tttatattga cgaagttcgt gatgaagttg attatgatga gttgccaaat
     6181 aatgaaggaa aaattcgtat tcgtacgaaa aactatgaag aaactgtgaa gggaggtgag
     6241 aacgacaatg ccgaaagcta aaaaagtacc atttcagttt actaacgaaa ttcagaatgg
     6301 taagcacatt ctcaccttga gtggaaatgt acagaaaaaa tattggcgtg atgatgatgt
     6361 cataaacgct aaagatattc gtgactctct agatactgta accgatgata ttgtaatcaa
     6421 gttgaacagc ccaggcggtg atgtgtttga agggattgag atttacaatt atttaaaaga
     6481 tcacccatca aatattactg tagaagttac agggcttgcc gcaagcgcag caacgtttat
     6541 tgtcgctggt gccaatgatg tgattatgaa tgttgggact tctttgatga ttcatgaagc
     6601 ttctacattt gcttggggga ataaacagga catccaaaaa acgttaaacg cattggaaac
     6661 gatcgatgat tctatcttag ctatttattc agataagact ggccaatctg ttgataagtt
     6721 gcgtgaatgg atgaatgaag aaaaatggtt tacagcagat gaagctgtca aatatggatt
     6781 tgccaattct gtgaagcgtg aagaaccaca aaatgaacct caaaatattg cagcgatgat
     6841 tcaagatgct gttgccgttg caatagcgaa tttaaaggaa cctgttacta ataaaatcga
     6901 acaagaatca aaacaaaaat cattaatcgc acgattgcga aaaggagaat aaagatatgt
     6961 taaaaattac agataaaact gcagatgcaa aaaaggcatt taatgctatt tcttcgaaag
     7021 aagatgcaac acccgaacaa attaatgctg ctctagaaga atatgtaacc gccattgcag
     7081 aagatgccgg caaacaggtg cgtgctgaat atgaagaatt gaaaaatgtg acggacaacc
     7141 gtgtattaga ggctcgtgga attcctacac taacagcaga agaaacaaag ttttataatg
     7201 aagccgtaaa atctggaggt ttcgattctg acgtagtttg gccagaaacg attttggaac
     7261 gtgtttttga aaatcttcaa aacgatcatc ctattttagg cctcattaac tttacaccaa
     7321 ctgtaggtcg tgtcaaagta attagagcac gtcgtaaagg tgttgctgtt tttggaccat
     7381 tgcataaaga ccttgaaggc caactagatg ctgaatttgg tgcaactgaa tttgttcaac
     7441 ttgcgttgac cgcatttttc ttaatttcta atgatacctt agatttagga ccacgatgga
     7501 ttgaccgttt tatcaatctt tctttaagtg aagcagtacg tgatgtttgg gctaaaaaaa
     7561 ttattacagg ttctggtaaa gatgagccaa ttggattgct taaagatcta gatggtgcgg
     7621 tcacaagtgg tgtatatcca gataaagcta gcgcagggac attaactttt gcgaaagaga
     7681 agattgttct tgaacttgca ggggtgatga aaaaattatc taaatacaca tacaaagtag
     7741 atggtgacga tgaaggtgaa gtgaaatacc gttctgttgc tggacgagtg tatctaattg
     7801 ttaatcctgt tgattactgg gatatcgtag ctagagttac atttgcaaat ttaaacaatg
     7861 tgtatgggac tacgatgcca tttatcgctg ttgatcatat catcgaatct gtagacgtac
     7921 cagaaagtaa atgtattgca tacgttgata aagagtatga agctactcaa tcaagagctg
     7981 aaaaagtcta tgtatataaa gaaacttttg caatgaaacg cgcaactttg tatgctgtcg
     8041 acatgcttgg taatggctat ccaacaaata atgatgcagc acaagtatat gacttggact
     8101 ttacagcggg ggagtaatgt cccctgcaat tggtgagata acaccgacga ctgatggggc
     8161 agtcattaat ctttcgtaaa tggggttaga gtatgaacac tgaagaattt ataaaagaat
     8221 ataaggcccg atttcgtatt ttccatactt ccgaagatga ggatatcggg aaacagttag
     8281 agagcgggtt tgccgatata aaatctctaa tcggcagttt tgatccttcc acttatgaga
     8341 aaggaaaaga attagtttat gaacgtactc ggtatctacg gaacgaatca ctagagtact
     8401 tttacgacaa ttttcaaaca atgattatgg atgcatcaat tgatctggca ggtgctggat
     8461 atgccaatta aatcaaatta taagaagcct aaaattgtag ctggtgatct aaatacgcca
     8521 gttacttttt ttgagtttca acctaataag ggtccagaac ctggtgatga agaaaaaaag
     8581 gaactgtatt tttgtacagc actagtttac aacccatcaa tgaaagatcg tgacattctt
     8641 ggcacaaaag aaggcgttac gataaaaata cgggatcccc atcaatctta tattcctact
     8701 aacaaacata aagtaaggat attagattat cgatatggag ataaagtttg ggaaataatc
     8761 gatgtctcac cggatttgga agaaaattca attgtaaaga tagtattaga attaattact
     8821 taaatttggt gataaatgat gagtaacgta aagtttgaag gcgttcaaga aacaatccaa
     8881 gctttagaaa aaaagtatgg agaaaagaaa actaagacat taacaaaaaa agcaatcaat
     8941 gttggcgctg aaaaagtaga aaaacagttg caaacggaca tgaccgtttt taaagacaaa
     9001 ggttatacga tcgctgaggt tgttcgcaag aacgctactt atagagatta caaggcagaa
     9061 gctgaaatcg gttggaatgg tccacatcaa cgatatagaa tcattcattt gaatgaatgg
     9121 ggatatacac gaaagggcaa acaaattaga ccgagaggat ttggtgtaat aacaaaatct
     9181 cttaagaatt ccgagctgtt gtactttgat gctgtaggag aggaggttaa aaagagttta
     9241 tgaaagatat gcttaatatc atttacgagg ctctttgttt gaatgagtat attcattcaa
     9301 tgacctacaa cgagaatacc gagcaatatc gtattaaatt ttatgagcaa cctgaaaccg
     9361 ccgataaaac aggtccaatg atcacaattc gtcctgtaga tacacctaac gaagcgtacc
     9421 acggaagtaa caaagagctt tctgtggagc atatgataca gattgacgtt gaatcgaaat
     9481 atagagcaac atgcaagcag attcaatatg agataaaaaa agagatgaaa aaactaggct
     9541 ttggccaaat ttccggtcaa gggttagatg aatactttac tgaaacaaac cgctttgtag
     9601 acgcgcgtcg ttacgaagga aatacaaaaa tttacgatac cgaatattag aaattaagac
     9661 acgaaaataa cgtgtctttt tttattaccc aaaattagga ggaaaatttt atgactttag
     9721 taggattcaa aaaaatgaca atcggggtct ttgacagcac agggaagatt ccagcagcaa
     9781 atttatatgt aattgaaggt gaacaaaaca aaggggcgac tgtatctgct gaaatcagtg
     9841 ggctatcaaa agaaccatca aaagtttacg gatcgaacat cgcttactat gtctctcaaa
     9901 aaggaacagg cgatgtttca gctacttttg gattgttaga tttaccaaca gaagtaaatg
     9961 acaaaattct tggttacaaa accgatacaa acaaaatcag cttcttggga gaagatactg
    10021 agccaccata ttgtgcaatt ttgatggaat cggaggattt gaacggcgat acagcaatgc
    10081 ttgcgatgtt caaaggaaaa ttcagtcgtg aatctattaa tcttaacact acgacaaatg
    10141 aagctttcga accagaagca gaagaatatg tattttcagc gattgccaat gatgttgaag
    10201 gtgatgctaa aggccaaaca gtcgcaaaat atgttggaga cgaagaagct tctattactg
    10261 gtttgaaaga gatggtattt cctgcagcgg gggagtaact agccccgttg ttggagcaat
    10321 cactcccact attaatgggg caacagttga attaagttag gaggataact atgccagata
    10381 catttaaaat ctataaaaaa aatggtactg aatttacaaa agtcgcagag ggtgaaagtc
    10441 cactttctat tactggaatt gcagcaaata ctcaagttgc taaaggagat tatcaagcta
    10501 cgcgtgtggt cggaggtcaa gaatcagtaa aagtagatat tcctgcattc accactcttc
    10561 caatttcagt tactggtgtg acgttggata aaacgacagc tgaagttgaa caaggcggaa
    10621 ccttaaaatt aactcctact gtaacaccag caaatgcaac agacaaaaca ggaagctgga
    10681 gcagttcgaa tacagctgta gctacggtta ctggaggcaa cgttacagta aaaacagacg
    10741 ctgaagttgg cggaactaca gaagtttcat ttactactac ggatggcggt aaagtagcaa
    10801 aatgtactat tacagttatt gaaaaagcag cgggctagtc ttcggactag cttttttatt
    10861 tggaaaataa tggaggaatc taaatggcaa aagtaagaat tgaattgaaa gaagaatcag
    10921 gaaagaaagt ctatgaaaac atagacacaa cagggaaaga ttatcgaaaa gctttagaaa
    10981 caatcaaaaa gctaaatgaa gacggagtaa tgatttggaa tcgtttagac atttatttgg
    11041 attttgctgt aggtatcttc agggatagca aattgacagc tgaacaaatt cttgatggct
    11101 taccatctga gaaggtaatg agcacattgg acgacatcct aggagaagta atggggattg
    11161 aaagtaatcc agacccagac gcaaaaaagt aacacccgaa gaagcagaag aaatgtactt
    11221 aaacctatgc agagagttag tgaaagcggg atggtcacta tctgacatag aagataattc
    11281 ctttgaaatg atgatgaaaa ttgcgtgcac ttcgcctaag aaagagaaac aaaaagaagt
    11341 tgatcttaaa gacttcttga aatccatgta gagaggaggt aaacatatgg caaatggaaa
    11401 acctttaggc aatatgatta tcaaactaga cttagacagt tcagcttttt caaaaggtct
    11461 agccggagct aaaaacgctg ttaaccatca aatgaaagca atgaaatcac agatgcaagt
    11521 aatgaactct tcaggaaatt ctctcggtgc tttacaagca aaatacaatg gtttaggcgg
    11581 agttctacaa gcaaatgaaa agcaagtaga attgctcact aaggcttata aggatagttt
    11641 tgactctaac ggaaacgcta ctgcttcgac tgctaaatac gctaatcaat tgaatcaagc
    11701 aacagctaga tcagcaagtt acgaatctca gatgaaaaca actgttggac agattgctcg
    11761 gatgaaagtg gaaacggaag ggtttacggg gaaactaaag gctaattctg atgtattgat
    11821 taattctggg aaaaaaattg aagcctttgg taaaggagtt tctgggatag gatctgccct
    11881 aactgttgga gtaacagctc caatagtggc aggcgcaact gctgttacta gtgcagctat
    11941 ttcatgggaa agtgcttttg caggtgtaaa aaagacaaat gatgaagttg tagactcgaa
    12001 tggaaacgta gtttattcct atagcgactt agagaaaggt ctgcgtaatc ttgccaccca
    12061 attacctggt agccatcaag aaatagcaaa tgtagccgaa gcagccgggc aattaggtat
    12121 aaagactgaa aacgtagtta gcttcactaa aacgatgata gacatgggtg aatcgactaa
    12181 tatgtcggct gaaactgcgg caacttcttt agcacgattt gctaatatta cccaaatgtc
    12241 tcaaaaagac tttgataaat taggatcagt cattgttgat ctaggtaaca attttgctac
    12301 aaccgaatca gaaatcactg aaatgggatt gcggttagcg ggagctggta aacagattgg
    12361 aatgagccaa ggcgaaattc taggatttgc tactgctcta tcttctgttg gtgtcgaagc
    12421 tgaagcgggt gggtctgcct tttcgaaggt catgatccag atgcaactag ctgtagaaaa
    12481 aggatttggc gcttttgatc agctgaaaca gatggcagaa gaacaaggtg ttccgtgggt
    12541 taatttagta aatgctgtga gagacggtgg aaaatcgtta aaagcagtat cagagcaaat
    12601 gggattcact tcatctgatt taaagaaaat gtataaagaa gcggacaatt cgaaaagttc
    12661 gcttgaaaat tttgcgaatg tggcaggtgt tacaagtgaa caattccaga agttgttcaa
    12721 gagtgatcct tcagaagcaa tcatcaagtt cattcaaggg ctgaaagatt cagaaaaaca
    12781 tggtacttca gcaatcaaag ttctggatga catggatata aaagaagttc gactacgcga
    12841 cagtttattg agagctgcaa atgctagtga catattcaaa ggagcaattg aaaaaggaaa
    12901 cggcgcgtgg aaacaaaata cagctttaac aaatgaagct aataaaagat acgaaacaac
    12961 tgaatcaaag ctaaagatat tgaaaaacga agtgattgat acagcaatcg atcttggagg
    13021 tccatttgta gacgcgttgc gtgatggtct aaatgtatcg aaaccattga taaaaaactt
    13081 agggaatatg gcgaaagctt tctcagctat gagtgatgat caacaaagaa acattatcaa
    13141 atgggttgga ttagcagcag cagctggtcc agcgctgaag cttttaggaa ctggtacttc
    13201 agtaataggt aaagttactt caactactgg aagattgacg aaaagccttg ttgatttagc
    13261 agcgaaagca gcagaaaaga aggctatgga tgcctttgca tcgtcggtaa caacagctgg
    13321 tgctgcagct gcaaagacag caggagctgg aggtattggc gggttagtcc ctatgatggg
    13381 aaaaacagct agtgctgcaa gtactgctgc gggcgccagc ggaataggcg caatgacaac
    13441 gtcactaggt ttgttaggtc ctgctctact tggtattgtt ggcgttggtg gcgcactggc
    13501 agttggctat ggagcatgga aattatttgg tgaagaagct tggaattcct cgcaaagagt
    13561 aaagcaatgg ggaacagatg tcggtgagga gatagatagt actcttgacg gagtacagaa
    13621 gaatattgaa ggtgcaaatg gtcaattcgg tcttctcaaa gagggtttta ctgttgatga
    13681 tgcaaataat atggcggcta atttcgcaac cgctggaaca tctttggaaa catctttaac
    13741 gggtagaata aaggcattag acgatgcact aaaagggcta cctcaatcag ttcaggatgc
    13801 catgaaagaa gtagctgaat cagaagaaga aaccttaggg caatctcttt ctaaaataaa
    13861 agacaacaat gaagaaataa aaaaaatcag agaaagagcc gcaaatcaga acagagactt
    13921 gactgtttca gaactacaac agatacagga tctaatgaaa ggaacttctc aagcgtatgt
    13981 ggatacgctc gatgtttctg ctaaagacag aaaaaagata ctatcagcca tgaatggtga
    14041 tgttgcacaa gctacagaag aagaagcgaa gacgtggtta caatctttag cgaaacaaaa
    14101 agctgcaact accgaaaatt atagacaaat gcttgaagaa aaaaaggcgg ctttaaagga
    14161 aaagggagta agcaaagaat tcattgatgc attagaagat gaaatgaatg attacatcaa
    14221 aactacaaat caaggattcg atagccaaat agctgctata acaaataaat atccacaatt
    14281 gattaacgaa gtgagtttat caaatggtca attatttagc aaaacggcag ctgctatgga
    14341 caatactggt cagtatacaa aagtttttct cgaaagtaat gaaaagattt tagcccaagc
    14401 gaagaaaaca tcggatgaaa tagcagataa tgccgaaaaa aatgcagaac aacttggtat
    14461 ggtagctgat gaggctacgc atgctggatc aacttggaat ggcttagttt tagacgaaaa
    14521 aacaggaaaa gtaaaaacta atgtacaaga agtagtgaat gaagcaacga aggatttcac
    14581 taaatggaat gaattgaaac ctgttgttca tgatgcgaaa ctaacaacga atgcaaaaga
    14641 tgtcatttcc atagcggcag tacaaaatgg ctattgggat gatatggaat ggaaagaaaa
    14701 agaagcatta ttagaagatg agtgttctaa aaatgtatac aaggctttag aaaatgctgg
    14761 taaatggaat gagttagata ttccttctaa agaagcaatc ctaacttcaa atactcctga
    14821 agttatgggt gaaactctca tgaatttggg attatgggat acttacaaac ttcaagtgaa
    14881 agatttagat ttgaatgcgt ataaagtata cgatgctatc gatggatcag aagaaaaatt
    14941 aaatagttgg gctcaattag ataaccccac aaaagaattg ttattagata actcagattt
    15001 ttctacaaaa ctttttaatt ccgaagaaat gtttaaacgt tggaatcagt tatcatctaa
    15061 tgcaaagttt ctttcggcta ataatactaa atttacagag gttcttttat catctgacag
    15121 aatgtggaat caatgggtaa accttcctga taatgagaaa agattacttg cagataacga
    15181 agatttagct accactgttt tatcttcaga agaaatatac ggaagatgga ctcaattacc
    15241 agataatcaa aaatatatgt tagctgacaa tactgactta gcaaacaaaa tattcgcttc
    15301 agaagaatca tggaatgcat ggaaagcaat tccagacaca gttaaaaact tgcttggtaa
    15361 taatattgat ttactggcta aagtgaaaga cgggactgta agtgtagaag aatataacaa
    15421 agttatgcca cttcttaaaa ctctatatgg agataacgtt aatttaaaag aggcaatcag
    15481 tggggctaaa agctctatag agaattacaa caaaaatatt tttcctagtg ataaaactgc
    15541 agtaggacac gatgaggctt cacaagctgc taaagatgca tttgaagcgt tcaacatctt
    15601 ccaaacaaaa ataccagata aaattactaa aactgtatca gctgacttta taggtcctat
    15661 gccgaaaaat gcgaaaggaa caaacttcca tcctggcgga gcagctatgg taaacgatca
    15721 aaaagggcct acttatgaag agcttataac cttgccaagc ggagaaactt ttatacctaa
    15781 aggacgtaat gtcgttttag acttaccaag aggttcgaaa gtactgaacg ccactaaaac
    15841 taaacgtcta gtgcctaaat atgctgatgg tataggaaac ataacgacgg tttcatctac
    15901 gctaaactta gatgctttaa ttttagctat taacgagcta acgacggtat tgagaactca
    15961 actgatggca ggaacgactg atgctaaaga tactaaacta tcagatgaaa caaaagttgc
    16021 cgatccgatt attccggaca gtctttctga aaaatcagat cagtatctag gaattggagc
    16081 tcaatggtta actaatctaa tgaatggttg gaactcagcg gttcctcaat atatgaatag
    16141 tgaaatggtt tttattacaa actatctcaa tgcgctaaaa cttcaaaaca atcctaatta
    16201 tacgcaagga gcaacatgga ataagaactt actgaatgga tggaatagtt taactgggac
    16261 ctttattgct acaatcaatt cattttgtaa tcaagcaatg gtcacgctta gaaactacaa
    16321 cactcctatg tacaacaatg gccgaacgtg gcagcaaaat aatcttaatg gttggaattc
    16381 attgtatgga tcattcatag cccgtgtaaa tcagcttggt aacgattcga ttaacaacct
    16441 tcgttctaaa agtggtggtt tttacaatgc agggacattc ttgttgcaat ctttaataaa
    16501 cggaatgaat tcgttaggta attctctttc tactacaatg aacaatgtcg ctaatacaat
    16561 ggttggtggt ataggcaaag gagttaatgg cgtcatatcg ggagttaact atgtgcttaa
    16621 ggaagtcgaa tcaagtaaga atatcggaaa ctggccaatt ccacaatacg ctaagggaac
    16681 tgatggacat cctggtggac ttgctatgat taatgatcaa aaaggcctag ttcacgaaga
    16741 atatgttcag atgccagatg gacgtagttt tattgccaaa ggtagagatc ttcttgttaa
    16801 tttgcctaaa ggtgctcagg tcttaaatgc ttctttaaca aagaaattaa aggaacgttt
    16861 aaatattcca cactacgaaa aaggtattgg caatttagat attgttgatt tgttagatga
    16921 tgaaaaaaga atgttagaat tcttaactag caaagttgat ttttcaggca ttaatgaacc
    16981 atggcttgat atgactaaat caggaacgtc attaatgtcc aaagctgcaa atacaatgct
    17041 tcaatcaaaa ttaagtgaat tcttcactca tggcaatttt gatggtgcag taaatgccaa
    17101 tggtgtttat caatatttag ttgatgtggc acagaaagta atgggtaagt tccccggact
    17161 tacagtaact tctggatatc gagcaggaga tgcctactat cacggaaaac ggcaagctat
    17221 cgacttggct tatccaggta tttctggaga tccgagatat acagcagcag caaattacgc
    17281 ttttgaaaaa ttcccttcaa aaattgcgta tgtcattacg aatggacgtg tacgtgaccg
    17341 catgggatta tctggaacaa ggtcaagcgg acaatggacg aattggccag atggcgatca
    17401 cttcgatcat attcatttaa acggttcgat ggggtctggc gatatattca atggaggagt
    17461 tggaggaagt ggtgttgcac gctggcgttc ttatgtgtct aaagcattga aaatgaatgg
    17521 attaccagct acagcagcgt atataaatgc ctggatgtcc caaatccaaa cggaatctgg
    17581 tggtaatgag aaagcaatcg gcggcaatga tgggctcgcc gaaggcaatg caacaggatt
    17641 acttcaaacg aaacccggaa cttttgcagc taacgctttt cctggtcacg gaaatattat
    17701 gaacggattt gataacatgt tagcagcaat caattatgct aagaaacgtt acggagttgc
    17761 aggtatgttg caagttatcg ggaaaggaca tggatatgcc aatggcggct tgattaccaa
    17821 agacggacta tatcgagcag gagaaggaaa taaaccagaa atggttattc cattgacaag
    17881 aaaaacaaga gctatcgaac ttatgggtca agcattagcc ttcctttctg gaagcaataa
    17941 aaaagaaacg aaaagtgaga ttaattctgt agataataga catatcctac aagcgataga
    18001 aaaacagtct cgtatgacaa atgatctgct atcattatta ctctcgtttt ttaaaagtaa
    18061 caatcgatca gataaagaac tagctttaga tattcagaaa attttaatca ggaggacata
    18121 gtatttgagg acagtaatat taagaaataa attgaatgaa gaaattgatt tgtccacaga
    18181 agattatttt gctactgaaa tgtcgaatct aggttttgaa gttacaaaag agcatattgg
    18241 acaatggggg agtttcagag aaataggaga gagtattgaa ttgtctgaat ttcaatcatc
    18301 agttatgatt tctgttcacg gatttagaga acaacaatta tatggttcat tagttgaatt
    18361 tctggctaat ggaccttttg aactagaatt ttcttttgat tcagaaacaa tgatcaggaa
    18421 atgtagtttg aaatcattat ctaaaacaga aattgattcc caaagctcat tgctaacaga
    18481 tactttgaat ctatatttta cttcaagctg gtattctatt aagcgtgaaa aattaataca
    18541 gaattcaact tctctcaata cacgtggaaa agtttactca tttgttcgac catatgtcta
    18601 caccaaaaat gtatgggaaa agaaaggtgt atttaagttc agtaataatt ctgtatattt
    18661 gacaaatagt acagagcgaa tgtctccgtt aaaaataagg attattggag agtgctcaaa
    18721 tccatattgg gaagttatcc aaaactctaa agtagtagct tcagatggct attttattaa
    18781 tatgaaagac acacaaatat tagaagtgtc tagtttgcta gaagaccaaa cggcaatttt
    18841 aaaagatgta ggtggagttg aatcttctgt ttatcaacaa caagatttta ctaaatcaaa
    18901 cttcgtccaa gcgcctctag gagaattcag cgttgttttt catgttggac atgcaaatgt
    18961 agagattgaa ctttatgagg agcgtgatct attttgattt tagccattac cctctttcac
    19021 cgagatatga atttatataa cgaacgttta atttcgactg agttcgaatt tggaacggat
    19081 gaaattggag aagaagtaag taattttatt acagataaat atgtccatgt aaaatcagga
    19141 gattttctac tagcaaaatt tattcctagc gggaagattg cttattttgg agtgattagc
    19201 tcgcaagaag acaaaaaaat aaattgcaaa ggattactca gtttggccga tagcgagata
    19261 ccgactgtaa aaatatcagg tgctaattat gaagagcata ttcgacgact gattgaatac
    19321 tacttattga acgatccaac aaaacaacta aaaaatattt tggaggtaaa aacagaaagc
    19381 gttacttctc attcttatca agctgttgac acaaataaga gacgattgaa tgcatatata
    19441 ataaatgggt tcaaaaaata taatgttaaa tggtatgtca aagagattaa aggtaaaaaa
    19501 atatatactg gtattcgagc agttgatgat tcaatttata ttaaagataa ttcatctgag
    19561 tttaatgact gggatgtttt tgtgcaatct cctggaccag ggaacgagaa caaattattg
    19621 atcgtagata aagcaatgaa agatatagaa aatcccttgg tattgtctac gtggtattta
    19681 gatgacgaaa ataatcttac gcaagattca acgaataaga acattgcatt gcctaccgtt
    19741 aatttagtaa atatatatga tcaaactcaa gaagatagac cttcttatga agaggtagct
    19801 aaatcggagc ttaaaggcaa cgcctattca catgaaatta aagttaatgt tgtgctaaat
    19861 gctagaaata tcaatgtaga aaatatcgaa acaggaatgt tagcgactat tgtttacaaa
    19921 gagcagactt acaaatctgt tctaactgcc tggcgaatat ccagcagtaa aaagaatata
    19981 gaattgacat ttggtaatgt ccgaagtcgt tttatggatt attttgaaga taatggggga
    20041 taaaaaatgg ttagcaatgt ggatggatat caatttgaaa acgtgaaagt aagcgcagaa
    20101 aatgatgcta gactttatca cgttttatat aatcggaaaa atcaggttat tgatggttac
    20161 gatcagtcta tgaatttatc ttcaagcgga ttaacagtaa aagttgctgc aggagcagcg
    20221 attattcaag gtcgtatggt cgttgttcga caagaagaaa gtataacagt tccagcaaac
    20281 tcaagtggtt atatagcatt aacagtggat ttgacacaag aagttatacc tggatctatt
    20341 cttccagaat cggaagaata tgaatggact aataatcaag tcaagctaga atttataaca
    20401 aaagttataa aaggtaattt aaacaatggt gataaggtct ataatttacc actatgctca
    20461 gttaattcta ctggatcaac tgtttcaatc tcaaagatat cggatagtta cgagctgact
    20521 ctctctaaag gagaaatttt gtggaggggg actgcgttaa tgcatgatac tcaaactgtc
    20581 caaccttcaa aaaaaatttg gcagacagtt tcaggttttt tgttagtatg gctcccatac
    20641 gaaaacgggc aagcaattga ggatagatat gtaactacgc ctttttataa ggagcgtata
    20701 acttttacta atgttttagg agaaattgtt tcaggatttg atgactatca caaaaagtgg
    20761 tttagtaaac gaataaacta taattcgaat actaatatat ttacaggtgc agcaagcaat
    20821 gcaagtggag ataacgcaaa tatggtgctt aggtatatag tttcttttta gttaggaggt
    20881 atggaagagt ggcaaattta gaaattaaat tatctgcaaa taagagacag ccttatctac
    20941 gtcaccgtgt tgttggtaga gttggtgatg gggggctcac aacaatcaat gtacaacttc
    21001 ttgaggaaga tgaaattaca ccttttgtaa ttaatctaaa cggtactttg aaatttgtgg
    21061 gcgaagtttc aaacggtaac tataccgagg gagaaccaga aataatcgat tcgactaatg
    21121 ggttaattag ttacacgttc actaagtcaa atttcagcac gagtcatcaa ttcaaacaag
    21181 catattttga atatgtagat cctaacggca aaaaagtaac ttttcagaac ttcatcatag
    21241 acgtgttaga acgagtagat attaattcgg agcaagcgaa atactatatt tcttcattgg
    21301 aaaaattaca gagtgaaatg caaaccactt tcaatcagtt cattagtgat aaacaggtcc
    21361 aatacgatca aatctactcg aaatataacg aattagtaag attgataaat gaatcagata
    21421 agcaagtaaa tgatcgtatt gataaggcaa ataatcgtat tgatcaaacc aatcagcaaa
    21481 tcggcgatct cggcaagctg aaaaagatgt acagtaacag cattga
//
LOCUS       DAJSBN010000044        21488 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJSBN010000044 DAJSBN010000000
VERSION     DAJSBN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.044"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            169..870
                     /locus_tag="ONT86_001951"
     CDS             169..870
                     /locus_tag="ONT86_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR2352719.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1479..2495)
                     /gene="tsaD"
                     /locus_tag="ONT86_001952"
     CDS             complement(1479..2495)
                     /gene="tsaD"
                     /locus_tag="ONT86_001952"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR2352720.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2492..2956)
                     /gene="rimI"
                     /locus_tag="ONT86_001953"
     CDS             complement(2492..2956)
                     /gene="rimI"
                     /locus_tag="ONT86_001953"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR2352721.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2963..3505)
                     /gene="rimI"
                     /locus_tag="ONT86_001954"
     CDS             complement(2963..3505)
                     /gene="rimI"
                     /locus_tag="ONT86_001954"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR2352722.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3489..4211)
                     /gene="tsaB"
                     /locus_tag="ONT86_001955"
     CDS             complement(3489..4211)
                     /gene="tsaB"
                     /locus_tag="ONT86_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR2352723.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4402..5382)
                     /locus_tag="ONT86_001956"
     CDS             complement(4402..5382)
                     /locus_tag="ONT86_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2352724.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5406..6890)
                     /gene="galT"
                     /locus_tag="ONT86_001957"
     CDS             complement(5406..6890)
                     /gene="galT"
                     /locus_tag="ONT86_001957"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCR2352725.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6902..7891)
                     /gene="galE"
                     /locus_tag="ONT86_001958"
     CDS             complement(6902..7891)
                     /gene="galE"
                     /locus_tag="ONT86_001958"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR2352726.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8139..8306
                     /locus_tag="ONT86_001959"
     CDS             8139..8306
                     /locus_tag="ONT86_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HCR2352727.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8368..10179)
                     /locus_tag="ONT86_001960"
     CDS             complement(8368..10179)
                     /locus_tag="ONT86_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR2352728.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10176..10541)
                     /locus_tag="ONT86_001961"
     CDS             complement(10176..10541)
                     /locus_tag="ONT86_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCR2352729.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10704..11099)
                     /locus_tag="ONT86_001962"
     CDS             complement(10704..11099)
                     /locus_tag="ONT86_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR2352730.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11117..12079)
                     /locus_tag="ONT86_001963"
     CDS             complement(11117..12079)
                     /locus_tag="ONT86_001963"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCR2352731.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12079..12291)
                     /locus_tag="ONT86_001964"
     CDS             complement(12079..12291)
                     /locus_tag="ONT86_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCR2352732.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12312..13010)
                     /locus_tag="ONT86_001965"
     CDS             complement(12312..13010)
                     /locus_tag="ONT86_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR2352733.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13030..13572)
                     /locus_tag="ONT86_001966"
     CDS             complement(13030..13572)
                     /locus_tag="ONT86_001966"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR2352734.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13704..14708)
                     /locus_tag="ONT86_001967"
     CDS             complement(13704..14708)
                     /locus_tag="ONT86_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR2352735.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14705..15694)
                     /locus_tag="ONT86_001968"
     CDS             complement(14705..15694)
                     /locus_tag="ONT86_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR2352736.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15691..16497)
                     /locus_tag="ONT86_001969"
     CDS             complement(15691..16497)
                     /locus_tag="ONT86_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2352737.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16663..17619
                     /locus_tag="ONT86_001970"
     CDS             16663..17619
                     /locus_tag="ONT86_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2352738.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17696..18214)
                     /locus_tag="ONT86_001971"
     CDS             complement(17696..18214)
                     /locus_tag="ONT86_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR2352739.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18302..18451)
                     /gene="rpmG"
                     /locus_tag="ONT86_001972"
     CDS             complement(18302..18451)
                     /gene="rpmG"
                     /locus_tag="ONT86_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR2352740.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18679..19125
                     /locus_tag="ONT86_001973"
     CDS             18679..19125
                     /locus_tag="ONT86_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCR2352741.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19320..21215)
                     /gene="asnB"
                     /locus_tag="ONT86_001974"
     CDS             complement(19320..21215)
                     /gene="asnB"
                     /locus_tag="ONT86_001974"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCR2352742.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6469 a         4558 c         3701 g         6760 t
ORIGIN      
        1 attcgcttca aaacaaatta ttcaatacca ttgtatattt tttacataat aacaaattat
       61 atttgtaaag tattaaaact tgtttctaat gttggtatgt taaaacttta caaattcttt
      121 aaaaaaagtg acaataaata tagtgaacaa taaggagagg actttttaat gcgtgttaat
      181 aaaatcgatt ttcacttttc aaaactcaga tatcaacctg attttgaaca tttctcggat
      241 gaagagttcc aacagatcaa ggaacatact gttcttcgtt cttataaaaa aggacagatc
      301 cttttcgacg atggtgatcc cagacaacgg ttgtatatct tgaccaaagg actcgttcgt
      361 ttagaacgtt atgatgaaag tgcaacttat tactactatg actatgtgaa tacaaataac
      421 ctttttccta tgtcaggaat gtttggagaa gagacctaca cttatacagc tcaagcgatg
      481 actgatattg aaacattcta ccttcctgtt tcctactatg aaaaattagc acgaaaaaat
      541 cccgaacagt tgatctatat gatacaaaaa atttctaaag tagttgaaat acatgaattg
      601 cgtatccaaa tcggattgac ctctagcgcc tttgaccggg taaaacaaaa tttattcatt
      661 ttgaaagaag aattgggcgt accaacaaaa gatggtcata tcaccattcc ttatccgatc
      721 accttaaaag aattagcagt gaatagcggc acgacacgtg aaactgctgg acaagtaatc
      781 aaacaactac gtgattccgg gctgttagat tatgacaaaa agcgttttac tttctgtaag
      841 acagctacta ctccagctat gagagaataa cgcgaacaaa aataagcagt gaccctgata
      901 aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg gcagttttag
      961 atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat attcactgtt
     1021 tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg atctagatta
     1081 ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga acggataatg
     1141 ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg taaaaaatag
     1201 ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac ctgtcaactc
     1261 ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa ttgttccttg
     1321 tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc aaaagcttgc
     1381 atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc ctgggctgtt
     1441 cttattactt gatgcagggc agctttttca caacctcttt attcagataa aaccaagctt
     1501 ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac atgtgctgct
     1561 gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac tagttctacc
     1621 tcagggagtt tagctgccaa agcatcctgc aatccttcac gcaacccttg attggccgct
     1681 acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg aagtgtttta
     1741 gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt tttgtctaga
     1801 tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt taatccgcta
     1861 aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta attatctttt
     1921 ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc taatactctt
     1981 ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat gatttcatat
     2041 gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag aagtgccatc
     2101 aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc catatggttt
     2161 acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga aataccaatc
     2221 agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa atcttctgcc
     2281 gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc tacgtgatgg
     2341 cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat ttgagaagca
     2401 acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt ttcatcacaa
     2461 cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt tttaactttc
     2521 tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt ttacctaata
     2581 tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga cgtacttcta
     2641 aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac aaatgccgtg
     2701 catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg acttcatcta
     2761 ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa agcagataat
     2821 cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt gaaccatgtg
     2881 gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca tcaaaatctt
     2941 tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata tccaacgcat
     3001 cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac cccaatttcc
     3061 gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc gtctcacagc
     3121 gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt cgtttacgct
     3181 gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac gctcttgaac
     3241 cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga ggaagcggtg
     3301 acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta tagacttctc
     3361 gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt acgtaacgac
     3421 gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa aagcttctgg
     3481 catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt ctccaaccat
     3541 ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt ctctgcaact
     3601 ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc ccatgctgga
     3661 actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa tttgtgacaa
     3721 tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa caaatcagaa
     3781 gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca gcggtatgcc
     3841 ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt atccacaccg
     3901 atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt atctaatgta
     3961 taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga accgggacca
     4021 tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc aaacaattgg
     4081 tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg tatttgtcca
     4141 agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt atctattcct
     4201 aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat tgtagcatac
     4261 ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata catgattatc
     4321 tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta tcactccatt
     4381 ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact ctgtttccac
     4441 aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg tctccactgc
     4501 tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg ttacatattt
     4561 cgctacactg acatcattga agcccatcac agaaatatct tctggtacca ctaaaccttt
     4621 ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa aaagcgcatc
     4681 aggaacatgc ggattcgttt ttaaatattg cttcactgct tgatatcctg actcgacaga
     4741 aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa cttgttgaaa
     4801 tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt ctgttccagc
     4861 aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt tttgaacact
     4921 tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag aatctaccaa
     4981 aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga attttcctag
     5041 tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt tcaataaagt
     5101 aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat aataaagatc
     5161 ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct tagtatttgc
     5221 tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa ttttttgctt
     5281 tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa cagtagcagg
     5341 agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct cctccttttt
     5401 tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc atctcgcttg
     5461 aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt ttgtacgaca
     5521 tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc tgcccacgga
     5581 cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac ttctactagt
     5641 tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa cccaatgttc
     5701 tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg atgttcttct
     5761 gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct tcttcgtgca
     5821 attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat agatactgtt
     5881 tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat aagcaagttt
     5941 ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc tgcgatgact
     6001 tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat aggaaattca
     6061 tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat tggtaagtca
     6121 gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag tctagcaaat
     6181 gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga atgttcattg
     6241 taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt cattcgaata
     6301 atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc gttttccatg
     6361 caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc gatttgtttt
     6421 ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata ctccgactct
     6481 gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt tttcttactt
     6541 aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg tgcaaagaat
     6601 gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc aaaaatctct
     6661 tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac aagctgatcc
     6721 aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag agcatcttct
     6781 cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat ccatccgcca
     6841 gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat atctcattcc
     6901 cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt ttgataatcg
     6961 ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt tcacttgaag
     7021 cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga atttcttttc
     7081 ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg ctgctgccta
     7141 aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa atatgtgcag
     7201 caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt gtatcataat
     7261 cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa atgattggga
     7321 caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt gctccggcta
     7381 cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac cacttcatca
     7441 ttttttccat catcaattta ctttcaccat acggattttt aggattagct ggtgtatctt
     7501 ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag aatacgattt
     7561 tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat acgttgttgt
     7621 tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca gcaaaatgga
     7681 ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta tcccggatgt
     7741 ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg agcaaattat
     7801 ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc gaaccgatat
     7861 aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt agtaaaattg
     7921 tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga aaacaaacaa
     7981 agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta aacattttca
     8041 agggtttttt gcaaaggttt acaaacaaac gtaaaactag cctttttttc tcttttgttc
     8101 cataatagat ataacaaata ataaatgagg tggattcaat gaaaaaattt gtatctggaa
     8161 tattagtagg aagtctggca acagccgctg ccgttgctgg tttggttgct tctgtgaaaa
     8221 aaacagtgat cgatccaatt gacgaaaaag aagcaatgat cgaagaaaac cgtaaaaaag
     8281 caatgcgtaa acgcgtttct cgttaataat tatttaaaaa tacgcatgaa gactttctaa
     8341 aaaacagata agtcatcatg cgtattttta tttgtcagtc aatataagtt gtgctatttt
     8401 atctgcctgt ctcagattcc atggatctgt ataaggtcta gctactgctg cagttagcca
     8461 atccacacct tcaacaggta ttccatagca ataaaagtca gactggattt cccctgaagc
     8521 atcaatcacg cgattcgtac ctcgctcaac atctatagct tctgaatgat aacttcctgt
     8581 attatctgaa aatgaaaaag ggcgaacaag tttgttttcc ttcaaatttt ttatgacagg
     8641 attacttgaa cgagaaaagc ttgtttgtgg caatcgagct tcaatcaaat agcgactgct
     8701 gatttcttta tcttgtagag aaataacata gcggttgtgc ttttgctgaa tatcaaatgg
     8761 cggttcgatc aaatgaaaaa tacccgcatc tatcagtgct gctaattctt tcgtccgaat
     8821 gattggcgga ccgatcgtta aaaagctatt tagatgagta aaccatcccc atagtatttc
     8881 tttgtattcc ttagcagaaa aaacgtgcca ttcaatagcc tgccggattg gatcacgcca
     8941 gtcttttaat gcatcaaatg ttgaagcaaa tgctcctgtg caattgcctt tttccgcttc
     9001 ttttatctgc ccttcaataa atattcggct agcttctgaa aagggctcat tctgataggc
     9061 aagaggagtt tctaaatacg accaatccca tctataaggg attagtgctg gatgctttcc
     9121 gagacactcc tctgaagaga gggagagaaa atcatgctca aaatttttct ttggaatcgg
     9181 caagtgatat tcttcgatga ttttcttgta ataaaacaac tcaacgtctt ttttcagata
     9241 gtcaaaaaat gtttcgccag tcaaaaatcc cgactgatgc cattgctcca ggttttcttt
     9301 tgttagtaac cgtggccatg ccattgctcc gccttgcttt tgatttcttc ctcgagggta
     9361 ataagggagc ccccttcttg atccactgta tacaatcggt tccttgcctg atggatgata
     9421 gaccagtttt cccttttcgt tctcgaagcg ccctcctctt gatactgtaa acaatccgac
     9481 ataatcaaaa aaacttaagc ctagacctct taaaaagaca ttttctttag gcggtatctt
     9541 cgaaagatct acatctgcag gattagaagg aagctgatac agtaaaacat ttttctttgc
     9601 gtagtctacc cacactcttt cttcttctgt ctcatcattt tcccagtgac cgcttgctaa
     9661 aacaagctga tcgatagaat agctttgctg atctgtctgt aaaagataac catcttttct
     9721 tttgcttact ttttttacta aagaataaac aaactgaata gaagctggaa attcttcttg
     9781 caactttgag aaaaaccagc gttgatagag tccgtataaa catcgagtag attgctgatc
     9841 tttttccaga cgattgattt cttctataat tagttgctta tttttggtta ccgtctgttc
     9901 gatataatct gtagcaggtc ctttactcca ttgaaacaag ttgggtccct gtgttaccaa
     9961 tcctccactg gacaatgttt catctgtaaa aagcgtcact tgttgagaaa cagaattcat
    10021 taataactcc ggtggctgat tcaaccgcca aactcgacct ccaggaccat tgggatcgaa
    10081 aagaaaaagt tcaagagaat gtctgtcctg attattatgg agaatccttt ctgctacact
    10141 taatcctcta ggtccggcac caatgatccc tattttcatt tgcatgcctc catttcgata
    10201 aattttttca ataaaacagc atggttctga tgttcatttt ttgcaccata aacaaaagta
    10261 atgactggat gttctttcat aagttgactg agtttaaaaa tagcttgctt cttttcttct
    10321 tcgaaaatca attcttgctg gtatttcttt gcaaatgaag caaatcgttc tggtacatgg
    10381 tcaaaagttt tgcgtgcttc attactaggt gcaatttctt tcaaccacag atctaactgc
    10441 gctttttctt ttgaaattcc tcgtggccag aatctatcca cgagtattct atagccatcc
    10501 tctttttcag catcaaggta aacacgtttt aatttgatca tagttgtttc cttctttcta
    10561 ccttttcgct tagcttatat atgtagataa tagcacaaaa taaaaaaaat gaacggcaca
    10621 actatccatt gcgccgttca tgtgatcttg gtcaaaatct aatgattgat tcggttttta
    10681 aagctgtcat acaatcatct gttttatact tttttacttt tgatttttcc agaccatccg
    10741 tcatatccac ccttaagtaa ataaatctct tgataacctg ctttacgtaa tttatttgct
    10801 gtacggatgc tcaaactttt cttttgatcg taaatataga caggctggtc ttttcgaata
    10861 gatcctaatg aatctttcaa aacagtgtaa gggatgtttc ttgcacctaa aatatgccca
    10921 gcatcaaaca catctttttc gcgaacatcg attacttgtg cttttctcat tccttctcga
    10981 aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca ttactcgtag atagacttcc
    11041 caaaggatga ttgcaagcac aatcaatagc aaaatgatat taattaccca taaaaccatg
    11101 taatgcctcc gtttttttaa gcaaatttca aggctaatga ggcagcacca atgacaccag
    11161 cttcattacc aagctctgct aatttgattt ttgttgattc tcttacttgc gggaaagtaa
    11221 attctttaaa atatttctct acacggctac gtaaaaattc tcctgctgcc gatactcctc
    11281 cacctaacac gatacttgac ggatttaatg tattccccaa gtttccgcaa gctaaaccta
    11341 agtagaagca tacgcgatca acaaccatca atgcaaacgg atcgtcttct tgagccattt
    11401 caaagatatc tttacttgtt acttcttctc cgttgtctag cattgctttc agttttgaat
    11461 cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac cacacctgta gcgctagata
    11521 cagtttctaa acatccgcgt ttaccgcaag tacattcgaa cccttcagga tccacagtga
    11581 tatgtccgat ttctcctcca gctccgctca cgccgtgaat caaatttccg ccagcaacga
    11641 tacctccgcc aacacctgtt cctagagtaa cgaatacaac gtctggatcg ttttctccag
    11701 cacctttcca tcgttcacct aaagcagcaa cattcgcgtc attatcgata gcgaatttga
    11761 ttcctgttcc tgcctcgatc agttttttca caaattgaac ttctgtccag tttaagttat
    11821 acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc tggggtaccc atgccgattc
    11881 cgataaagtc ttcggctttc atattatata aattcaaacg atgattgatt gattcaatga
    11941 tttcaggaac gatatgctta ccatcatcca aaatattggt atcgatgctc catttttgct
    12001 gaatctctcc ttctggtgtt aaaattgcaa attttgctgt tgttccacct aaatcaatcc
    12061 caataatctt cttgtccatt acaaatttcc tttctttcgg ctttcttcaa ttcggtgctc
    12121 tctttttaat atgcgccacg ctgtcatgta tgtatcatga tcaatcaaac ggctatcata
    12181 cagattacgc aattcgatag tcattaattc aatgtcatag atgcgtgcac ccacatagat
    12241 aaagatgcca aattttttga acagctgctg tacatcatat aagctctcca tctttaagca
    12301 cttccttcat attatacaag taaaccatat ttttttaaac ccagtaaaat acatattaca
    12361 aaaagaaata caaaaactag tgtagaaaga atcctttggt gtatcgaata gcttgtctgt
    12421 tgtttcggta gacccaaaac attccctaac actaatccgc caattagccc accgacatgt
    12481 ccccaaacat ctacagaggt atccagaaga ccaaaaacaa atgtcaacac aataaataaa
    12541 agaaaatgtc tgactagctg ctggatcgct gggttatgtt taaaatgaaa ccctaagata
    12601 aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac caccagacag aacattcgct
    12661 tcgttaaaag caaagcttgc tgtatttccc attatcccgc tgaacatata aatcaagaaa
    12721 aaacgccaat ggccatagat tgcctcgatt tgctgcccca taaaatacag tatcagtgag
    12781 ttcattacga aatgcatcag tccaaaatgg atgaagattg gtgtcactaa acgccaccat
    12841 tcattgaagt gaacaagata aggaccatac attccggcat aataagggat ctcaagtcgc
    12901 ggaaataatt ccatcatgag gaagaccgct gtctgtatcc ctaaaaaagc ataggtccat
    12961 aacggtcgtc ttaaccatgc acgcaatttt atttgctgtt gataattcat gaaaacctcc
    13021 catttcattt tagtcaataa acaattgttg gatcttctga tcaaagctct ccggttgcca
    13081 ttgttcattc aattgttcac gaaaaacaag gctacatgtt tttttatgaa aattatttaa
    13141 ataccggtcg taataaccac cgccaaaacc gattcgataa ccggctctat tgtagataat
    13201 acctggtaca atgaggagat cgatcatctc cggcaaatag agcttctctg attgtggctc
    13261 aagaacacca aaactcgaac gtacaagaac ggactgttca tccatttcat aaaacgccat
    13321 ctgacgtttc ggcaaagttt taggtacaac tattttcttt tgttcttttt ctgctcgttg
    13381 gataaccggc tgtgtatcca gttcaaattc attcgataaa gtcgtcccta tcacttttgc
    13441 tgacttccag tactcgctgg cgaatagctg tttatagatt cgctgttctt tttccttttt
    13501 cttgccactt ttggccagat ttttcagctt ttcgattcct ttccggcgta atcttacttt
    13561 caattggtcc aaaaactcac ccttccttct ctcataaaat ggtataggaa aaaaagaaaa
    13621 atagcaatcg aactttcaaa taaatgcata aatagaataa aaagaagacc tagaaatgat
    13681 tggccttcct tttttaatga atttcaactg ttcagcgcgt gtttttgtct gataaaaagt
    13741 gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa ttcctgtagg tacttcccca
    13801 cttggtagaa tcactctagc aaccatatct gcactaagca gaaaaatgga accgaacaaa
    13861 ccgctcagaa caaaggtcca gttattttct ttacgtacga ctagcttcgc agcatgagga
    13921 gctagtagtc ctacaaaaga taaactacct gtcaccgcta cactaccgct tgccaaagca
    13981 acagctaaca acaaaaggat tttttgactt ttagccacgt taaggcctaa tccttgcgcg
    14041 cgttcttgac caaatgatag caatacaatc ctctgtgcat aaagtcctcc tagcggaatt
    14101 aagataaccg cccatggaag caatgcccaa acataaggcc agctcgcacc ccagattgat
    14161 cccgctaacc atgcattcac aaatccataa ttttccttag ataattggat cgtgcatagc
    14221 aatgttaatg cagaaaggcc ggcattaacg gcaataccgg acaataagat tcgattcata
    14281 cccaaaaagc catttcgatc ataggccgca agatagacac agcaagcagc taaaacacca
    14341 cctagaaagg ctacaactag caatagaaac ggcagcgaat agtgcgaaaa gaaacctaaa
    14401 taaagcatca ctaaaaatcc tgctccggca ttaatgccca atatactaga atctgccaat
    14461 tcatttcttg ttactccttg caagatataa ccagaaacgc caagacaagc tcctgctaat
    14521 aaactgatca gtaatcgagg aactcgaaat tctctaacaa tcagcgccaa agaagatgat
    14581 tctttcccta tgaacgtggc aaaaagctga tccaggtaaa taaaaggcgt accaatcatt
    14641 aaactaccta tagacaggat gacaagtaca aaaagcaaga aatagaacgt ccaatatatt
    14701 tttttcaccg ttgttccctc cttacttgta tcagtaaaaa cggaacgcca atcaatgcaa
    14761 tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat cctcgctgta aaatccgcca
    14821 acataaccaa tccaccgcca actaaaaaag agacaggtag aacataacga tagtccgttc
    14881 caaccattcc gcgaacaact ggcggaacca acagcccaat aaatgaaaca ggtccgacta
    14941 acgaaacggc aatggctgct aacactgcaa ctagacagaa aacaatcaac cgcgttcgtt
    15001 taggatgact tccaagagct aatgcatgtg tttcactcaa atgaatcaaa gatattgctt
    15061 ttcctaatga aaaagaaacc aaaacagcaa gtatatataa aggaaacaat actgctaatt
    15121 gtttccatgt gatattggca gctccgccta caaaccaaaa agcaagatcc tgctgcagat
    15181 taaagaattg ggtaagtgct tggctgatag cagtaaaaaa agaactaata gctacgccag
    15241 ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa ctgcaaaaaa ccaaatacga
    15301 gcaaagttac agcaaaagaa cctgccaatg acattagaaa cacagaaaaa tcagaagctt
    15361 taggccaaaa agcaaaacta agggccattg ccaacgaagc acccgcgttg attcctagta
    15421 agcctgaatc tgctaaagga tttcttgtca ccccttgcat caatgcaccg ctcaatgcaa
    15481 aacaactccc tacaagaaat gctcccgcca cacgaggcaa acgaatattt cgtatgattt
    15541 ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg ccaaaaatct gctttttcca
    15601 cagtcgctgc gccatttgaa agagcaacaa aaaaaataat caaaagaaca aaacagacca
    15661 aaagcaggag tctatttttc ttcatagtcc tcataattga tcctcctgct cttttggcaa
    15721 caaatcatag gttagaaaaa ctggtttatt tgttcttgga agcgtgacaa tcgttgcatc
    15781 aatctcaaat atttttttca tacactcttt tgtgatcact tcttcaggtg taccttctgt
    15841 gatcaactta ccgtttttca ttgctaataa ataatctgaa aaacgtgaag catgattaat
    15901 gtcatgaatc gacatcaaga tcgttgtttg tttcatttga ttgatttcag tcaataactg
    15961 taggatctct aattggtgtg ccggatctaa aaatgttgtc ggttcatcca agatcaagat
    16021 atctgtttcc tgagcaagcg ccatagcgat ccagacacgc tgggtttgtc caccagacag
    16081 cgttgcgata tcccgcatac gaaatccagc tagacctgta gcatccaacg cccactcaat
    16141 tgcttcatga tcttgcgtgt ccatgccact gaacagtttt tgataagggt atcgtccgta
    16201 agatactact tcttctactg tcatacctgt agggtgttca ctagtttgtg ctaataatgc
    16261 aatttttttt gcaagttctt ttgaactgta tgtagagata tcagagttat tgacaatgat
    16321 ctttcctgat ttcacaggta gtatacgggt aaaacttttc aataaagtag attttccact
    16381 tccatttggc ccaatcaaac tagtgatttt cccttcagga atcgtaacag acatgttttc
    16441 aatgattttt ttgttttcgt agccggctgc aatgtcagct gctcttaatc tcgtcatttc
    16501 acccatcctt tcgctgaaaa tcattctcaa ttatgacgct gtttcaacat ttcgtcaagc
    16561 gctactttca tatctatagc caaatgaagc ggttgacagc gttcgaaata tcaggaataa
    16621 taaccaatga gaaagattct caattatgtt aggaggaaag aaatgaaatt aaaaacaaaa
    16681 gtattcacat tatctagcat attgctgctg gctggagtac tgagcgcttg ctccccctct
    16741 tcagataaag aaacagctac aactgccaat tcgacgactt catccaatag tactcataca
    16801 gtcacagata cacttggaca taaagtagag attccaaacg cgcccaaacg gatcatcggc
    16861 agttacttag aagactatct gattgcacta ggggaaaaac ctatcgccca gtggactgtt
    16921 ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa aggatgtccc aacgatttct
    16981 tatgaccttc cttatgagaa agtgttaagc tttgagcctg atttgctgtt gatttcttct
    17041 tccgctactg tcgaaggcgg gaaatacgaa caatatagca aaatcgcgcc aacttatgtt
    17101 gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa aagatgtcgg caaagctttg
    17161 gcaaaagaaa aagaggcaga aaaaatcatt tccgattatc aaaaatccat caagaaaact
    17221 cgagaagaac tagcagataa aatcgagaac aagacagctg cagtgttgtg ggtgaccaac
    17281 aactcagcct ttatggtcag caaaaatcgg tcaagtggaa gaatcgtata cgacgatctg
    17341 gaatttggtg ttcctgatct agtggaagag gtctccaaaa aagccacttc tgactggtct
    17401 gctgtttcct ctgaaaagct agctgaatta gatgctgact acatcatcct tgtgaacagt
    17461 gacaaagatg ctgccatgtt caatgaacca agctggaaga atctaaaagc agtgacagac
    17521 aatcatgtac tgcaattcgg acctgaatct agttggctat ataacggacc tattgccagt
    17581 caggaaatgg tcaatgatat caaagaaaaa ctaaattgat tttaactaaa aaaagcatct
    17641 tcacgattag tcgtgagatg ctttttttct tattaacaaa ctagacgcat agattttatt
    17701 cttcttttga agcaacccta ttttttactc tttcccattt ttgaaaacga tgggcatata
    17761 gattttcttg atccgttatc ccctcatact cctctataag ggcgaagtct gaaaaatcaa
    17821 tattcccaat atacgtatcg ccttcaaacg tgctatcgat caatgttcgc cagatccgac
    17881 cagtgtaagg aagaagttct tgaaaaatcc gactaccacc cgaaacatag atatcttctg
    17941 agatcgtttg actatacgac aaaagttcat cgatcgaatg cattacttcc gcattttctt
    18001 tcgtgcgaaa atttggttgc gttgtcaaca cgatgatatg gcgataagga agtgaaagat
    18061 cccccattcc ttcataggtc tttcttccca tcactaaaat tcgatccatt gtatgttcgc
    18121 gaaagaaacg catatcattt ggcaatcgcc atggtaacaa accatttttg ccgatcagac
    18181 catttttatc ttgtgcccac atggaaataa acatattatc cgctccttat gattttagat
    18241 gttttgagat aaaaaaagtc tcaattcttt gaccagcaag gaattgagaa ctttaaaaac
    18301 attattttgt ttcacggtgc aaagtaactt tgcgttcgcg tgggcaatat ttttgttttt
    18361 ccaaacgatc aggattgtta cgtttgtttt tgcttgttag atagttacgt tctttacaag
    18421 aagtacactc taaagtgatg tttacgcgca tgtttttccc tcccattttt acaagaatcc
    18481 tctaagatga ctctcagact tgattatctt accatgattt tttctagatt gctagtgttt
    18541 ttcacgattt tgttaagtaa aactacttga cgataaatac aacggtttta cttctcaaac
    18601 aaaatctttt tctttttgta taaataccct tacacttgtt tatatagatt atgtttttta
    18661 ataaaggggg atactaatat gccatggaac atgaaggact ttcctgcatc aatgaaaaac
    18721 ttagataaat tgacaagaaa aaaagcaatc gacatcgcaa atgcattatt agatgaaggg
    18781 taccctgata accgtgcgat cccaattgcc attgatcaag caaaaaaatg gtatgaagat
    18841 gctagtgaat cagaaaaacg aactttcgaa aaagaaaaaa atccaagcaa gacggatgaa
    18901 cacgatacga atcctcgtgc tggaaaactg cttgattcag atgtgatcgt agaatatgag
    18961 gaagaacaat ggatcgtgaa atctaaaggg gcaaaaaagg caagcaacca ttttgataca
    19021 aaaaaagaag caatcgaaaa aggaaagcaa gtagcacaaa acaaagaatc aactttgttc
    19081 atttataaaa aagatgggac aaaagaaaaa gaaatcgatt attgaacata tgtaaagggg
    19141 ttgtgacgaa aatcgtgtca caaccccctt atgttcgaaa caatggtgtt cctctgccaa
    19201 ctccgaacca taagtcataa tatctataaa acgggaagag ctgttttttg aaattgtttc
    19261 ttatcactca gtgttaaacg gtaggagaaa acctcttttt tattgatatt tgattttcat
    19321 caaacagtca catagtcgat tgcttctgct tgcggaatcg tttcatctac aaagtagact
    19381 ttataccatg tcaagaaact gaagattgtc cagatttttc tttgctcttc tttacgtcct
    19441 tcgtgatggt catctagcaa cttcatgatt ttttcttgat caaaaaattc ttttgcaaaa
    19501 tctgcttcga ataatgcttt gacttgttcg tagccgtgtt cttctttcaa ccaagctttg
    19561 attggcacag ggaatcctaa tttcacacga ttcgaccatt cttccggaag atgtttgttc
    19621 gcagcttgtc tgaaaatatc ttttgtgttg ttttgattca ataaaaactt acttggtatt
    19681 tgttgagcca atttcatgac ttcgatatcc aataaaggta ctcggacttc caaagaactt
    19741 gccatagaaa gtttatctgc ctttaacaag atatcttttg gcatccattg atgaagatcg
    19801 acatattgca tcttattgac ttcatctttg attccttctg ttttctcgta atgaacagtc
    19861 atgatttcat ccacagatgg tgctgtttga tattttggtg ttaacacttc tgatgcttct
    19921 gattcttcga acactcgagc atgaccgata aaatattctt ttgcaggagc aagtgattca
    19981 tataaatgga tccgtccatg gaagtttttc atttttccga ttttacgtcc taaattatag
    20041 cgtgttcctt ttggtaattt tttcaatcct tgagcaatca cacggatgaa ttttgaattc
    20101 gtatggaatc catacgtttg gtatcccgca aacagttcat ccgccccttc tccagattgg
    20161 actacccgca cactttgtga agcaagattt gctaaaaagt acaaaggtac gcaagaagga
    20221 tttgaatcag gttcatctaa gtgatactgg atcaaaggga atgctttgaa cgacatgtct
    20281 ccgtcgatca ctgctgctgt attatctaag tccaatagtt cggttagttt tctggcttca
    20341 atcgcttcat tataggtttt atcatcaaac ccgattgaga aagaatgatc tggtttcaat
    20401 acagaggtta cataactgga atcaaccccg ctggataaga aagaaccaac ttctacatca
    20461 ctgaccgtat gtgcttcaat agaagcttga acggtttcgt cgattttttc gatccattct
    20521 tgtcgggaat gcgtttcttt tgtttcaaaa tctgcatccc aatattgctg aatgtctatt
    20581 tttccatctt tatacgtata ataatggcct tcaggcaaac gatagacatc tttgaagaat
    20641 gtttcgccgt ttaatggaga atattggaaa gtcatataag gtttcaacgc ttctttgttc
    20701 aattctttgt taaaatcagg atgaggcaga aaacttttga tttctgaacc aaacataaat
    20761 gttccgttca tttctgcata ataatatggt ttgatcccaa agtggtctct tgcaccgaat
    20821 aactcattct tttcattatc ccaaatagca aaagcaaaca ttccgcggat cttttgcaat
    20881 aattctgttc cccattcttc gtaaccatgc aaaaggactt ctgtatctgc atgtgtttga
    20941 aaaacgtggc cagctgcaat caattcttct ctcaaaggct ggtaattata gatttcgcca
    21001 ttaaaaataa tgatctttgt ccgatcttca ttgtaaatag gctgagtacc gccctctaag
    21061 tcaataatac ttaatcttct gaatcctaaa gcaacatgtt tatcgatata ttctcctgaa
    21121 ctattaggtc cacggtgaac gattcgatcc atcattgtat taataattgt ttttttgttg
    21181 tccttgtcgt taacaaaacc tacaataccg cacatttaac tttcacatcc taatataaaa
    21241 ttactgttta tttcattcat atgcatctta tcatagaata aatagcggac gaatattttc
    21301 tctcttacaa tttcgtcacg tgaaaacaaa agatgaaagt ttctgttttt acagaaaata
    21361 aagtcccaat atctaattta ccatgttttc ttttttgagg ctaccttatt ctcttcgtgt
    21421 taaaaaaata ttagtggatg ctatttccaa aaaaaccagc aaaaaaagac acgacctaaa
    21481 gatcgtgt
//
LOCUS       DAJSBN010000045        21216 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJSBN010000045 DAJSBN010000000
VERSION     DAJSBN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.045"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(59..775)
                     /locus_tag="ONT86_001975"
     CDS             complement(59..775)
                     /locus_tag="ONT86_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCR2352743.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(1002..1544)
                     /locus_tag="ONT86_001976"
     CDS             complement(1002..1544)
                     /locus_tag="ONT86_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR2352744.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(1549..1776)
                     /locus_tag="ONT86_001977"
     CDS             complement(1549..1776)
                     /locus_tag="ONT86_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR2352745.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(1855..3735)
                     /locus_tag="ONT86_001978"
     CDS             complement(1855..3735)
                     /locus_tag="ONT86_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR2352746.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(3924..4538)
                     /locus_tag="ONT86_001979"
     CDS             complement(3924..4538)
                     /locus_tag="ONT86_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2352747.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            4680..5240
                     /locus_tag="ONT86_001980"
     CDS             4680..5240
                     /locus_tag="ONT86_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2352748.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(5545..5859)
                     /locus_tag="ONT86_001981"
                     /pseudo
     CDS             complement(5545..5859)
                     /locus_tag="ONT86_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(6113..7748)
                     /locus_tag="ONT86_001982"
                     /pseudo
     CDS             complement(6113..7748)
                     /locus_tag="ONT86_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8303..8866)
                     /locus_tag="ONT86_001983"
     CDS             complement(8303..8866)
                     /locus_tag="ONT86_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR2352749.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(8881..9924)
                     /locus_tag="ONT86_001984"
     CDS             complement(8881..9924)
                     /locus_tag="ONT86_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352750.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(10057..10461)
                     /locus_tag="ONT86_001985"
     CDS             complement(10057..10461)
                     /locus_tag="ONT86_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR2352751.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(10694..11329)
                     /locus_tag="ONT86_001986"
     CDS             complement(10694..11329)
                     /locus_tag="ONT86_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR2352752.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(11595..12647)
                     /locus_tag="ONT86_001987"
     CDS             complement(11595..12647)
                     /locus_tag="ONT86_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2352753.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(12660..13598)
                     /locus_tag="ONT86_001988"
     CDS             complement(12660..13598)
                     /locus_tag="ONT86_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR2352754.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(14049..14699)
                     /locus_tag="ONT86_001989"
     CDS             complement(14049..14699)
                     /locus_tag="ONT86_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352755.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(14799..15308)
                     /locus_tag="ONT86_001990"
     CDS             complement(14799..15308)
                     /locus_tag="ONT86_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR2352756.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(15509..16378)
                     /locus_tag="ONT86_001991"
     CDS             complement(15509..16378)
                     /locus_tag="ONT86_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2352757.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(16398..17519)
                     /locus_tag="ONT86_001992"
     CDS             complement(16398..17519)
                     /locus_tag="ONT86_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCR2352758.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(17521..17916)
                     /locus_tag="ONT86_001993"
     CDS             complement(17521..17916)
                     /locus_tag="ONT86_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR2352759.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(17935..18741)
                     /locus_tag="ONT86_001994"
     CDS             complement(17935..18741)
                     /locus_tag="ONT86_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2352760.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(18734..19522)
                     /locus_tag="ONT86_001995"
     CDS             complement(18734..19522)
                     /locus_tag="ONT86_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2352761.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(19536..20012)
                     /locus_tag="ONT86_001996"
     CDS             complement(19536..20012)
                     /locus_tag="ONT86_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2352762.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(20084..>21216)
                     /locus_tag="ONT86_001997"
     CDS             complement(20084..>21216)
                     /locus_tag="ONT86_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2352763.1"
                     /translation="KIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANR
                     IVERYIYDAIDMPLMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDA
                     QKKFGLLNNVSTRMAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCS
                     CASGLGTSFKLKKILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLN
                     PHLEDVEFFSIEDMILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTI
                     LNPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNE
                     TSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         6580 a         4350 c         3232 g         7054 t
ORIGIN      
        1 aaataaactg agcaaggggt tgtgataaaa gacatgtcac aaccccttgt ttatctcatt
       61 aatttgcttg tccataaacg atttgattaa agccattttt caattcatta ttcgctgtct
      121 cgttgaagta aagataatta tcgggattcc cctgattttg ttgtgtcacc aatacttttg
      181 gctgattttg ctgaaacccg aatagataga cgtgcttttt cagatatggt tctgtctctg
      241 catcggaata gacggctgtg atttgatagt ctgcttgacc tgtcccactt tctgaccatt
      301 ctgcagaaac aggcactcca ccaatagcca tcttccactc cccgttcaaa atagcttgag
      361 ggactttaag accatataag tccacactca tttgattcgt ataacttttg tattgttgtc
      421 ccattgtttg gccccatttg gtcataaatg cctctaattg tgcggatttg tctgcattcc
      481 acagtgtttg ttcaacttgt tcgctgcttt ctgaagagga agatgaagcc tctacagaag
      541 aacttgaagt agatgtttga gttgtttcag tcgttttgga agactctgac gaagtagagg
      601 tatctgctgt ttctgtactt gctgcagttg acttgctctc tgtgctagtt gttgtagtag
      661 ttgcaactgt tgctttcgct gtttcttttg ttgattcttt tttcgattca ttcccgttgg
      721 tacaaccagc taacaaaaaa gctagaccaa ttgttactaa aatccttttt ttcaattaaa
      781 aaactccttt cgattactcc cacttcttta ttctatcaaa taagcaatgt gattttattg
      841 atttttcgta ttttctcatc aatctttcaa atcttctaaa aactgagaag cacccgctga
      901 tgttgtctgt aatacatcag cgggtgcttc ttttatttta atcttcgtga ttttccaatt
      961 tacctcacgc tttatatttg tatttgaaga aacatagact tttagtcatc atcgtcttcc
     1021 tcatccaaat cttgtcggac agtttttcct tggagtcttt gtacttggta gatgatttct
     1081 ttattgaaac ttaaaagttc aacaagcaat tgttgcaagc catatttttc ttctttttca
     1141 gctaatttga ttgcccgtgt gataaagaca ttttggaaat caaaatcttt tgcaaagttt
     1201 tccaacattg ttgaggctgg taaatatttg ttttgaccat gttcgtaaat ttttccataa
     1261 cgcatgactt cttctaaagt agacggaacg atttcatttt caatcactaa ctcatgagag
     1321 attttattga accatctcgc ttcttgatca gcatattctg aaaaaagggt acttaattct
     1381 tgcgaagatg tacccaaaac atacaattgt gcttggatca atttcatttc ttctaattta
     1441 aaatttgcta atacatgccc caccatggcg ccagcagttg gtttatgatg gtcgatatct
     1501 gcttgttttg tttcttgaac aaatttttct tccactatat tcattgattt acgcctcttt
     1561 tacttttgct gattggactt cataacctaa tttttccaca actgcagcta attgttcagg
     1621 gtggacttca tctggatcaa agtttgcttt gattttgctc gcattaaaca aaactttcgt
     1681 attgcttacc ccaggctgtt gagaaaccgc atgttcgatt ttttgcatac agcttggaca
     1741 agctagtgta tctaattgca taataatttt tttcatcgta gttcttcctt cctttttctt
     1801 gatagtctta ttctagatta cagcttccat aaaaaaattg attcttatca agttttacga
     1861 tttttcttct ttgctttcgt gtccaaaata caataaacgc atcgcgttaa agataacgat
     1921 caagatacta gcttcgtgaa cgaacatccc actagccatg taaataaacc cggcaaataa
     1981 gccgttgagt aaaaataaga cgacaccaat ggcaatcact acattttcac gggtatttaa
     2041 aacagttttc ttagccaagc ggtaggcatg aaccaacgat tcaaaattag attgcatcaa
     2101 gacaacgtca ctcgtttcga tagcaacatc cgttcctgat cccatagcaa tcccgatatc
     2161 agccgcagcc aaagagggag aatcattgat tccatctcca ataaaagcaa cgcgcaatcc
     2221 ttgttcttgg tattttttca caaattgaac cttttcgtct ggtaataatt cagcatggac
     2281 ttcatcaatg cctaacatat cagctacata atcagcagtc atctgattat cacctgtcag
     2341 catcactaac tgttttgctc ctgcttgttt caacttagct aattgttcag cgacttctgg
     2401 tcgaatcaca tctgacacac ctatgatttg agtgattttt ttgtcgattg ctacaataat
     2461 gaccgagctg cctgtttgtt gtaattgatg taaatcttgt acttgctttg gtgtgagaga
     2521 aagtttgtga gcggcaatca atttggtgtt gcccgcacaa actgtatgac catcaatctc
     2581 agctgtgatt ccttgtccct tgattgtctg attatctttg actgctaatt gttgaaaatc
     2641 aatgtctttc tcctgagcat aatggatcac ggcgcgcccc agaggatgat cggataacgt
     2701 ttctaatcga gccactaaag ctaagctgtc cccttcatcc tctgtatagt tcttgatagt
     2761 ggatacagca gtagttcctt tggtcaatgt accagtttta tcaaagacaa aagtatcgat
     2821 tttggaaaaa gtatccattg cttcaccgcc tttgactaag atgccccgtt tcgctccatt
     2881 accgattcct gctacattcg aaacaggcgc accaatgact aaagcgccag gacaaccaag
     2941 aaccaaaaca gtgattgcta aacgaaaatc acgactaatc agaccaacaa ccaacgcaat
     3001 aagtaaaacg gttggagtat aatactgaga aaatcgatca atgaattttt ccgcatggga
     3061 tttggtatct tgtgcttctt ctactaattc gataattttg gcaaaggtcg tatcatctcc
     3121 gaccttagtt gcttcaatcg tcacgtagcc atctgctaaa atcgttccag agaagacttc
     3181 atctccactg attttgacga cttgtcgtga ttcaccagtc actgcggctt catccgcata
     3241 ccctttcccg tcaatgactc gtccgtccac aggaatagct ccaccagttt ttactaagac
     3301 gatatcgcct tcttccacgt catcgatttc cacttcttca gtcgttccat cttcttggac
     3361 aacactagca gtagatgggg ccatctcggt caaagccttg attgactccc gcgttttggt
     3421 caatgttttt tgttccagat agctaccaaa caaaaacaag aatgtaacga tagctgactc
     3481 cttgtattct ccgatcataa aggcaccaag cacagcaata gtaacgagta aatcaattga
     3541 gataactttg actttcactg cctgatacgc atggatcata ataggtatga ccgcaataat
     3601 agaagctatg gctagagcaa tgttatagaa caagacaaaa ccagtaaatt ttccgataaa
     3661 accaaaaata atcaacacag ctgataaggc ggtgatatga tttcgatgct cttggatcca
     3721 cttttgtaat ttcatctttc tccctctttt cctttcattc ttacgagact aagtatagat
     3781 acttatccat cataaaaaat tgaccactat caagtttagt caaaaaggcg gttttctttt
     3841 ggtagaatac actacaaaaa atagttagca agaagactca ctaaaagtga tcttgccaac
     3901 tattgtcgtt tctatcgaaa ttttcattga atgattttcc tgtacagcat acatcggaga
     3961 tctatttctt tatctaagat ttttatcttg ctaatttttt cctttagtct ttcaaatcca
     4021 cgtcgttcat agaattgata cgtacagcca ctatctgtaa aaaggaatat ttcttttcct
     4081 ttctctcttt tttcaaattc tgttagcaac ttggtaccga tgccttttgt tttcaaatca
     4141 ggatttgcag ctaaaaaaat gatctctcca tctggcgaat gcttctttaa atatgctgca
     4201 agcatttgtt catttgctgt ctcgtaagct cctttacttt ctttagcaaa tatattttgt
     4261 aaaaaatcga atatcttaac atatatttgc tgtccaaaag aacgatgttt ctttttttct
     4321 ccttttatct ccgccagcaa aacacctgcc aactcgtcct ctgcatacaa agcaagaatt
     4381 tgagttgctc gattcaactc catatacaaa aaatatcttc cgtaaagatt cagcagaagt
     4441 tcattttcca tatatctctc aaagttcatt ccttcaacag caaaatagat agcttttttg
     4501 taatctttct tttctaaggg tttaaaagtt agtttcatca tttcgcctcc tcaacttcaa
     4561 tgattgtgtt atacttacat tataatagac acagtgtcta ctatctataa tcaatcatct
     4621 ataagtttgt cgattaagcg acagctttgc tttgatatgt cggataagga gataggtaaa
     4681 tgaaaaagac agatagacgc gtcaaaaaaa cagaaaaagc tttggctgaa acactatcca
     4741 cattattagt aaataaaaag attcaagcaa tcactatacg cgaactaaca gaaacggcag
     4801 atgtacatcg ttcgactttt tatacacatt ataaggatat ctatgatcta tatgatcaat
     4861 tagaaagcaa cttttttcgg gatctaaatg aaattctctc ttatgatcca gcccatagct
     4921 atgaggaact atacactcgc ttgattgact atgtttacgc aaatactttt ttatatcgct
     4981 tatttatcct caaccatggt gatatttcgt ttcagaagaa agttaaacaa gttattgaag
     5041 aaaactatct gaacatatgg ctatatgaag acaagaaaac gattatcaca gaagaaatga
     5101 gattctttac tacgtaccac gtccaaggtt gtctatccgt tctcaatcgg tgggtagaaa
     5161 gcaattacaa gtactccaaa aaagaattat tagaactctt acagaaactg aatgatcaca
     5221 tagagaaaat catgccttga ataaaaacat actaagctta gttaagaaga accgtaacct
     5281 tctcgtataa caactgaatc ttatttagcc aaattgttta ctgaagattt tttgagtaaa
     5341 atttctataa ttcctactta ttgaaaagta gtttgtagtg ctgcttctcc ctctctaagt
     5401 gatatatctt gttgtaccaa tcaaattcac ctcatcatta ataaattact tttaggctaa
     5461 tttattacaa cttataggtt gaagctgtaa ccaatttata gtcatattat atttttatca
     5521 tccagtttat tgaattcttc tcgtttacct caattcattt tcaaggggaa tcagctcatc
     5581 atctaacatt ttacgccatg ctcgcttcat ttcttgtggg tatttaaatg gtttcgcttc
     5641 tggatgtttg gctttatatc cttcgtaatc cttactgttg ataattgctt tcatattagg
     5701 aattggatct tcacctggca tatattgcag attagaagca tgtaattttt tctcagaaac
     5761 aaaccactca ttggaaaaga catgaatcaa atcatcactt acttgtgtaa aatagcgacg
     5821 tttaatgaca aagatatctt cattgctatc tagtgtacct tcatttcttt gtgcttaaat
     5881 tcaaagataa ttttctcaat aaagattttt gattccagta agattccttt actcctttta
     5941 caacatagcg ttgtcctttg ttttgaaaca tcttcttccc ctctttgcca ttaaaaatag
     6001 cccaattctc tataaagaag attaagcttc attattataa tataaagttt aaatttggtt
     6061 tattacttac tttttcttat tctctagtaa gctaatattg aaaaaaatgt aatcagatca
     6121 ttatttttat atgttgtaac ttcctgataa gaaaactaat taaaagaaag aggtaactac
     6181 ataaactaat taaaaaagct aacttcatag ttggaggata aatggaagaa acttcaatga
     6241 ttgtttctcc ttgtgttcct tcaatcgaca ctagtcctat tatcaatctc tcttttttgt
     6301 ccatttactt ttgcgactgt tccccaataa tcaaatacag gaagcgttat tttttgtcct
     6361 ttagattcat gtactgtata gactattttc gaatccatca cttgataagg catagtatgc
     6421 cattccttgc caattcttac ttcatgattc tgcaaatgtt ctttgtattg taatgcttcc
     6481 gtgggtgcat agtcataaag tcctccggga aggagtgtcg tgtaacgaat aagattttct
     6541 tgattcatta ccaagtgatc tctctgagtg acaactttat gttcatagtt tgcttttgtt
     6601 gcagcagata cagccaacag taataaaaca attgccgtta tattaactac ttttctttgc
     6661 ttagctgatg tgctcttcat gaaaacgaac gataaaatca acgagccaaa cagtacttga
     6721 aagcctaaga ttcgccaagg aaattgaatc atttgaatgg gtgtattttg taataaagac
     6781 catggaaaaa atttggtgga taagagccaa gagactaccg cactaaggta aatcactttt
     6841 tctttgcgat taaatccttt aaaaaagaag agtggacaga tcaaagaaat aataccaatg
     6901 attcctaccg taaaggttgt caggtcatta ttcatcgcac caatcaaact atccgagaca
     6961 tttaatgctt ggtgttgtaa ggtcgtttta tctggctcat taatcgattg aaccatgatt
     7021 tgtcggaaca tcggatacca aaagtatgct gttaaaagaa tcgtcattcc tactgccttt
     7081 gacaactcca caatcaaccg gatgctttct ttttttcttg tttcacttac tacaaagccg
     7141 cctaagacta gtaagccaac ggctattaca ctcataaaag tggaaagaac atgtgtataa
     7201 atgagtaagc tcatactgac tgctaatgga attgcacatg gctttttctc ataaatcagt
     7261 gcgtaagcat aatacataac tatcggtata accgtaagtg caatcgcttc agcaatagca
     7321 gaacgatgat aaatatcaat cgtacggtac ccataaaatg tatataaaca gctaaatata
     7381 aaagccgcat cgattctttt taaaaatttt agtccataat gaaacatcag catgatagta
     7441 caaacattca atagccaaac atataagata taactaacga ttaaattatt acttatccaa
     7501 taaaaaatga ctgccggaaa aaaggtcaag tagggataaa aataattcac tccgctccca
     7561 taatgattaa acgtcgtaaa attgattgga ccagaaaaaa ttgaagataa ccctttgact
     7621 ctagcaaaat ggaaggcaat atcccccgat gtagaaatag tatgaacatt tctaaaataa
     7681 ttgattgaat aaagtccagc aataattgct aggagaatgg acaatattat ttttttcttt
     7741 acttgcatta cttaattata tactcctttc atggattgct ttatctagta taacttaaat
     7801 tttaaataaa tcgttattta tttttactct cacttaaaaa agcactaaaa ttataagaga
     7861 gggaggtaat ggtctgaaca ccaagaattg ttccaacatc gaattgctta actaatgagt
     7921 tcaaagctta attatgtggc tcttatattt accgagcgtg gattgcgatc tctttagcga
     7981 aacaaacatc tttcataaaa aaaacagcca tatccgcttt cttggatatg actatttact
     8041 actactttac tatctttcat tcttcttgtc tcacttatga taaggcgctg cgtatcatga
     8101 gtgaatgata ttaggaaaac ttcataaaat aaatctttta tacatttcca cttctagatg
     8161 gtcattcatg ttagaagaat caaactattt ttttactgtc gtaataatat taaaacacta
     8221 gaacaaatca attaaattcc ataaaatata aataatagac ctagcatcat ctggtataaa
     8281 atggatgcta ggtccgttat atttactcaa aattgattct acgaatagat cttcttatca
     8341 tttctatttt agtagtcatg cgttgttgtt cttctaaagg tggtaatggt ataagtagtt
     8401 tagataaatt tgtaccattt acatttggtt gcccagttcc gtaagaaatt ttttctaata
     8461 acttccaata taatggcgag tctaagaagc agtctacgta ttctgataat aatttctcaa
     8521 ctaacctaat tcgaatcaag taagaagcaa aaacagattc ctcacttatt tccttaacca
     8581 agaaactttt tcccatagtt cctcctgtcc ttgctatcaa aatgtcattt tcttccaatc
     8641 ttaaatcaac tagtttagaa ttactaatat cacagtaagg cacactggac caatttacac
     8701 gtccctcttg tatatctgtg attctcaaaa acttaacatt gccttgtttt ttcgccgaat
     8761 ctgtatagcc atattgaatc ttagtagaaa tacttcctaa ataaacccat agtcgaacat
     8821 cgctagatga tttttcttgt cgttctttat gaacaacagt gatcatcttg agttgtccga
     8881 ctatttttta gttgcagcaa tctttttggg acgcttattt gcatgaagca acgttgctaa
     8941 gtctagtaat aaaacattgg ctaacacagg attggctttc tcgcgatacg ctgcggcgcc
     9001 actttcaaac aatactgcta gacgatcttc tacaacaacg atttgttcca aatacggtgg
     9061 aaatttgatt cgttttagga tccggctagg agagtcccgc tcggacacta agagttccga
     9121 ggtataagga ccataagaac gactggctaa gagataatgg ccataaaaag ccacaccttg
     9181 aatcttatca gatgtttttc tgctcattgt cacggttggt tggattttgt ctttgcggcg
     9241 aattgctgtt ttcatttcag ctatcgtcgg caatagatag aaagtcaact gtccttcacc
     9301 ttttagtgtg aagttgccag ataccagttg atcattgttt tgagtcaaat acgatgcttg
     9361 tggaatctct gagagatcag ttgtcagcca ataagcgatt ggctgtcccg tctcttcaga
     9421 tgtatctgct aagatatcga tcaaacgtaa agcggctact cgtccagtag cagatcctgt
     9481 gattgtcaac cataaaagtt cccgttttgt atcatatact aagcccccta catgtggccg
     9541 tttaggtaaa atcagcagtt tgatcggctg tcctgtttct ctatccagta catgaataat
     9601 agaatgatgc tgatggtcat agcagtaggc agaaataaac acaaaatctt gagaaaaagc
     9661 caacccttgc ggcgtcatcc attgacaaac ctcattttcg ccggtttccg gcaccaccat
     9721 tcttgtttct gttaatccag gaatgttata ttgttttgct tcagcctttt cccaagctgc
     9781 ttgaacaata ggataccgtt ttaacttttt ttcaatatcc tgatgtatcc gttgctgctt
     9841 ccaatgctta aactttagga catagtaaac aagaaggcca atgaccagca acactaaaat
     9901 cattattgct acccgctgtg tcattttttt gcctccttgt tctattcgat caatcgtcca
     9961 tataccgcta ttaattaccg taactacatg tttcttttac tagcgtttct ttataggatc
    10021 tttcataatc tcatttatag taacattaag aatcccttat tctactatag tcacttccac
    10081 atcaatattg cctcttgttg ctttagagta tgggcaaaat gcgtgggctt cttctgcgat
    10141 tttctctgct tggtccttct caagtcctgg caaagaaact tctaaaacga ccccgatttg
    10201 gtagccccca tcttctaatt gaaataaaga tacccgtgct ttgacttcag aatcagactc
    10261 aagattattt tcttccagca tgtgttgaac cgcgccgttg aagcaagatg cgtatccagc
    10321 cgcaaaaagt tgttccggat ttgtcccttc actatggatt cctgggcggt cgatcttcat
    10381 gtgcatactt ccgtttggtg cctcaacttc gccatcacgg cctccacgat tgatcattgc
    10441 tgtttcgtac atttttttca ttgagatcat cctttctttc gtcgttctat attaagtgta
    10501 acgagtattt tttaatctga caaaggtaat gcttgcattc ggcgacatca gttagacaac
    10561 atcgatgatc agtgatagaa gcagtttatc tatacaaaaa aagaagggtt ctatgatatt
    10621 tggtgtcctt actcgccttg agtaacaaca ccaaatatca tagaaccact taatcagctt
    10681 cacactgctt tttctacttt cgtagttgaa aaaaacttaa acaaatcatc ggcaaatagt
    10741 taaagaaaaa acttaatcgt tgaaagaagc ccaattgctt aaagaaggca aactgactaa
    10801 tcgacggaat ccgtgctaat ccgtaaagac ctgcaattag taaactaata actgttaaaa
    10861 caagataaaa atggctaagc gtaccttgac cactttgacg ataaagcaga acaagtaaca
    10921 atgggaaaag tagaaagcct gcatagccta gcccagaacc tatattatgg atccaagtcg
    10981 atagattaca agagctttca ttcgtattga tactgaataa gccagtaaaa atacaatcac
    11041 ctatcccata taaaccaact gttgcaacaa caagtattgc aagtgttttt gatgttccaa
    11101 caaataggtg atacaacgca ggcaacgaaa gaacaaaaaa aagtccagat acaactgacc
    11161 agaccaagaa agcacgtctg actggactat ctacgtcacc aaacacacta attacggtag
    11221 tcatttgatt cagtttggga taaaaaaggc caaggatata cggtgtcgat aaatcactca
    11281 tcacacctaa taataaaaaa taaaaaccat atttttgcaa aaaaatcatt ttcttcccct
    11341 actttttagt gattattcga tacatccaac aatcaaattt tttctcattt taacacatat
    11401 ttttattcta gttgtctttt gaacaatcaa taacataaag gtgagtttct tctacatttt
    11461 aaaaaatcga atggcactga agagttcagt tgtcagcatc aatctcctac tcaaaaaggc
    11521 aaaaaaacgt aagtcagatg ttgttgacat ctcacttacg catccattga ttgaaaattt
    11581 cttagtaaaa tctactagcc taaaaatttg aagacttctt ctcgatcata cagtttaaat
    11641 actttctcgc ataaaaattg agtaattagt gccgcaaaaa cagggatgac taaccaacaa
    11701 agaacaacaa gaaagccatt tagccctgca tctaaagaag ccaacggacc aacaagtccg
    11761 accacgccga aaccagcaga agcaggcgta cctgaaatag aaaatagagc gacaggaata
    11821 gctgtaatca ctgcactaac taaacaagga ataagaatca caggttttct aaatagatta
    11881 ggcatcatca tcttcatagc gcctaaagca atcgcaattg tcacacctga cttattaacc
    11941 ttccaagagt tcactactaa aacaaaagtg gttgcagcta cgcccatcgc agccgcacca
    12001 gcagaaacac catctagttg gattgccatt ccgattgcta ctgttgaaat aggagagata
    12061 atcaaaaagg caaaggaaca accaatcaag atagacatta acagcggttg taaggtagtg
    12121 aagttattaa tcaatgatcc aattgcagaa gtaattgctg ttacaaaagg gaataaataa
    12181 aaaccaagta aacctgcacc tacacctaca acaatcggtg ttaacacaat cgacactgac
    12241 ccaaaactat ttccaatcag taaaatcagc ccgacagcaa tcgatgctgt cagcatcgta
    12301 ttaataatat cgcctgtacc agcaccaacg taaacacccg tttcacccaa atcaggtgtg
    12361 aatttgacta caccagaacc aacaaaagaa gctccggcta caaccatcat tctcatcgga
    12421 tcaaatccaa attgtaaggc aatcaaacca ccaatgatca aaggtgttgc tagttgaaaa
    12481 ataactgcta actgtgcgat cgttaccgca aagggatacg cactaaaata ttttaaaatg
    12541 gcacctaata ctgcattagg aatcaatcca ataatgattc ctgttgctgt accagcgagg
    12601 actttattca gaaaaatttt cggtgatatt ttttgtgaag tttccattca atctctcctt
    12661 tattttacgt tcaaactatc ttcttttgca tggacaagct cagtcaagaa atcacaataa
    12721 ggcgtcgcaa caccatattt cttccctttt ctagaaattg cgccattgat ataatcgatt
    12781 tctgttaatc gatggttctt aatcaagtct tgatacattg aaggataatg taacccaatt
    12841 gtttccgggt caaaacaaga ttcacaatgt gcaatgactt ctgggacatc tagttcaatc
    12901 ttctctactg ctgctacttt ggcaaactca ttgacaatcg tcgccaccat tttatgagca
    12961 gtcgatgttt ttcctagctc agccatatta acgtctaaga ttgtgcatag tccgttcatt
    13021 gttccgttca cacatgcttt gcgataaata gaatagtgaa tattgtcaga aaaatgagca
    13081 tttaatcctg attcagacaa tttgtctgct aatttttttg cagctgcttc ttttccatca
    13141 cctaaatttt gtaattctac tgaaccactt ccaaataatt tgacctgacc aggaccttca
    13201 agacctgccg tccacatggt atttccaata tagatatttt ccattggtac aaatttctca
    13261 ataatatctt catgcccgat accatttaat agacataaga cctctgtatc ttttttgatt
    13321 aatgattgga tatcctgcag cattttttcc agctgcatcg ctttggtaaa tagaataatc
    13381 agatcaactt gatcttcttt ttctacttcg gattgaagaa cgattggtag ttttgcttct
    13441 acctctttac cattaaaatt tgcttgtaat ccatgttctt tgatttgttg gacatgttct
    13501 gcccagccat cgattaataa tacttcgttc ccactttggt gaagcatcaa cccaaagcga
    13561 cttcccattg cgccagcgcc tgcaattgct attttcattg attcttcctc ctttttcttt
    13621 ctattatact catttgagaa tcttttcaca acctcgtttt gttattcttc tcttatttat
    13681 catgtttata tccaccattc aagttatttt atcagcaaat caataaaaac tggaatagaa
    13741 tcccaatttc atcgtaagag aaactagact agcaataaga aaatagaaac attcatagaa
    13801 aaaatctcac tcctctgatt ataaaagagg gggcattaac ctcgtgtata ctaaaacctt
    13861 ccattcattc gatggatgat tttctttttg tgaaaaaata ttcattgtag tacgatgttg
    13921 cgcaacacag acggatggta ttgcttatta tttattaaaa tcgaacaata cacttattta
    13981 aaaacttctt tcccagattt tcttccattt ttacagttat aactagtatt atcatgaaaa
    14041 atagcctttt attgtctatg ggaataataa tttaaaattg gtactttttt attaattagt
    14101 tcatcgccca ataatctctc cagtttctta gcatctcctg actcaaccgg aattatccaa
    14161 gcccagtcta aataattttt taaaatctca tcatattttg caaatatttc attgccatat
    14221 tccgtcctta aaattatatt ttcagtgcca gacaagtgat ttatattgag ttgataaaaa
    14281 agatctccat ctaaaaaaat atcattagaa gaagatttcc ccacagtaat gaccattcca
    14341 tcagctctat gtgttacata caaataattg tatgacctca tttcacctaa tgaatcaatt
    14401 atttcttgcc ctttagctct aatatattga tctctattac ctatctcact cttatttaac
    14461 aaaagttttt tcatgtcaaa tttaatcggc tgttttattt ttgtaaattc gcttctacta
    14521 ctaatccaat ccgagattaa tttttcaaca tgtcccagag taaactctga tttattcgct
    14581 aaatactttt tttgttgtat taaagacatc tttccccgaa gctcaaaatt gagcctacat
    14641 cgattctcta tctcttcaaa aagcatctta tcttcatcat tcttatattc atcattcata
    14701 aaaccaatcc agctcctcga ttaagataat attttaatct catacaaaaa ctagctctca
    14761 tcaaaacaac cattgttttg atttctcatg aaatttcttt atacttttct ccgattataa
    14821 attcctaacc ccttgagaaa attacgcata tatttatctc cgcaaggctt aaaatttcgt
    14881 tggccttctt ttctcaaaaa tgccccgatt tcgccttttg atggatagat ttctgcctca
    14941 gcaaaaacgt ctaaaatatc atcagtggtc atagacaagg cgatttttaa tttttttagc
    15001 aacacattgt tgatatattt gatttccttg cttttttgcg gcgtgtctac tatcggctta
    15061 tctttttgcg gaccacgcgc caagagaatc aggccattta ggaatgcgtc aaaaacttgg
    15121 ttgttgatgg ttttttcata cacattattt tctgcattat ctttgttctt atcctgtact
    15181 ttggtcagta ccgcctgagc ttcttcttta gtcagtggta caccgcctag ctcaaaagct
    15241 ttaatcaaat ccgtatcttt caaatcaatc gcataacgca atcgcaataa cctatcattg
    15301 ttattcattt gttcctccga agtatttagt ctttactttc ttacgtactt tttgtcttgc
    15361 taagattttt aggatcaatg ataaaaaccg catcacttat gataaggcgc tgggtatcat
    15421 gagtgaatga tattgctgaa tacaaatagt ccgaccacat taagttgatc ggacttcata
    15481 agcattttat tgactaatac ttttttgctt aagaattgat tctaacatct tttgctaaag
    15541 ttaagcatcc tatagttcct gcattatctg ctaacttagg tgtgacaatg tattcttcga
    15601 tatttggcgt atcgacatag ccgttaatta gttctaaaaa acgtctttgt actttctttt
    15661 tcagatgttc ttgttgcatg acaccaccac caaaaattat aatatctggt gaaagcatca
    15721 gggtcgtatt acaggcacat tgtgcaatat aatccgcctc gatttcccag aacggatctt
    15781 ctatagaaag atcttgtccc ttggttcccg tacgcatctc gatagcgggt ccagatgcca
    15841 acccttcgag acaatcatga tgaaaaggac aagtaccaga gaaagtatct cctgaatgtc
    15901 tcttaactaa cgtatgcccc atttctggat ggctaaaccc ttcaacaaaa atcccatctt
    15961 gtatagctcc tccaccaatt ccagttccaa cagtgtaata aacaacactg gataaacctt
    16021 ttccatgacc tgaaacatac tccccgtaac aagcagcatt cacatcggtt gtccaggcaa
    16081 ttggaatttc gaaatgctta ttcaacgttc caacaaaatc aaaatttttc caggcagttt
    16141 ttggagtaga ggtgatatgt ccataggtct ttgattcacg atggatatca atcggtccaa
    16201 atgaccctac tccaatgctt tctaattgtt ctttatagtg attaaagaaa tcaattacca
    16261 aactcattgt ttcattcggt gtagttgtag gaaaactcac tctttctaaa acctttaaat
    16321 catcattgcc aatggcacaa acaaatttag tcccacctgc ttctattgag ccatacattt
    16381 cttactccac cgtcctttta tctaggaatt cgttcttccc tgtctaatag tttttcgaaa
    16441 aactcatgtg gttccttttg gtaccaatag gcaacagaag aaatatcgtc ttgacgctca
    16501 taaagcttga tgtcatcatt tcctagggtt tggaaagtaa cacgtaaatt ttctttgaaa
    16561 ctaatggggt cgggtaaatg ccagcggtac aacccatgca tcggtaaagc atcatcgcca
    16621 aaaccatgaa ctggatttgg attaggtttt ccagcactaa aataatcccg ggtgtgatct
    16681 cttttagatt gaaaaggata gcccataaat agcgtattaa aattttgagc atatgccctt
    16741 tctccaaagt tctttttatg gaaagcccaa gcgccgccaa aataatcttc tgctcctgta
    16801 gatgttattg tagggaaatc ttcatctcca tctatataaa acttaaattc tccttccccc
    16861 caccagtatc tttccaatgc gcacaaagca aagtaggttc ctacataata cccagtgcct
    16921 ttgattccat caataagggt gtaatctttt ccttttgtcg tgatgggttc acggttccat
    16981 ttagcatgaa aatacatcgc atcatccggt acttcagtca atgcataatt aaatgtataa
    17041 aaaatgtgct taataaactc aggatgttca ttcgttaaag taatcttagc tttttttcga
    17101 aatggcatct caaaatagca attcatccct gatgttggat tcactacgat tggtaaggaa
    17161 ttcacttcgc atcttgtccc aaacccatta caaaaaaaat ctcctaatgg actttctaca
    17221 gaaggagtgg attcatcatc ccaataaata cgcaaaatta aatcacgtaa gacgaaactc
    17281 ccagcgattg ttttgtctgt cactgtcatc caaaaatgac gtaaaattcc agggccttcc
    17341 atttctgcta aagtaattgt ctctccagca tttatcggaa cagaaggtga cccttttctc
    17401 ccaattccta gatgactcgc tgtcattgcc gctttccctt tttcaccatt tttattttct
    17461 ggtgaaatag ttctactttc ctctcccttg tggagccata tattatttaa acaattcatt
    17521 ttattcgtcc tctatccttt cttcactttt ttgattattc atatagatga tttgttgttg
    17581 tgcctccgca ataattgctc caatatctaa attatcattt cctaactgca aagcgattga
    17641 cattgccaag ggaagattca ttcctgcaat tacgtgcact ctgtcagaaa tataggtaaa
    17701 aaacttttga tttacacttc ctccagccat gtcagttaaa acaattattt cgtctgtagt
    17761 atccactaac tctttaattt gttcatctat atcatcaatt tcttcgacat atgcacataa
    17821 gctactaatt tgattatgca tacctgttaa aaactctagt gtctctttaa aaccctcagc
    17881 tagtattgaa tggctagcaa tgatcaatct tctcataatt caaatccttt tcctttacgc
    17941 taatattcca aatgcagcca taaccatcgc aaaaacaata acgagtagaa tcaaaatggt
    18001 catactgatt tttttcttac tgagtagata atatgctgca gcagtaataa ttactggaat
    18061 taatgttggt aaaattttgt ccaacattgg ttggattttc atactaactt caccaaaagt
    18121 gaatttaagt ggcgttgaaa cactgataac tgaagcaatt aatgatccaa ctactgttag
    18181 ccccaatact gaagcagctt cagttaaata attaatcttt tcaccgtatt cagtaacaat
    18241 cttcattccc tgcttataac caaattcaaa gcctctcatt cggaaaaaga agattaccat
    18301 gattaaaatg aaccataaaa agattcctac aggattattt tctaaagcca tataagccgc
    18361 aattgatccc atgatggttg ggatcatcgt catcaaaaga gagtccccaa taccagcaat
    18421 tggacccatc gtaccaactt taaagtcatt aatcgcatct tttgcttcta atccttgctt
    18481 ttcctccatt gcaacacaag cacctaaaat aatggctgcc agccaaggct gtgtattgta
    18541 atactgaaag tgattattta aagcttcgat gtattcatca tcatctttgt ataattttct
    18601 taaaataggt gacaatgcgt aagtcacaga tgcaccttgc tgtgtttgat aattaaaggt
    18661 acaaactgac atcaaccaac gatagttcgc tttcctaaga tcctttttcg ttacttgata
    18721 tttttcgctc attttattca tcctccccaa taaacccaac atcagaagct cccgcagaag
    18781 cagtgatttg tgcatctttt tcagctctat gatacacatt aaatgctaaa gcgaatccta
    18841 tgatcgccgc tccaataata ggtaccttca aaaaagcggc taagaaaaat ccaacaagta
    18901 aataactaat gtatcgacct gtaggcataa agcgtaacaa catcgcaata ccaacagcag
    18961 gcaacatttt tccggctaat gttagcccat ccataaacca attaggtaaa acatccaaaa
    19021 cgtaattaac agcagactgt cctaaagtaa cggcaataaa tactggaatt gctgcagata
    19081 aaccaaagaa aattggcccc aaccacaaaa cactattcat tttcttaaat tctcgtttat
    19141 tcgcatattc ctgtgatttg cgaacaacaa aaccatttaa gattttgaca attacatcta
    19201 gttgcacccc taacatacct acaggtagtc ctattgctaa tccggcttct actccaagct
    19261 ggctaccaaa tgccgttgca ataattgcgc acgttccata gtccggtaca gatgagccac
    19321 caaaaccggc aactcctaat gacatcaatt gaactgtacc gccaactatc aaacctgttt
    19381 ttatatcacc catcaaaatt cctgtgaata atccccaaaa aactgcgtaa tttacaaaga
    19441 tcatcggaat cccataataa tcaacatgtt taataaatgc taatatagtc agcataataa
    19501 tttgaaaaat agtaaaatgc attatcctta ctcctttatc ctaattttga ttgaatttcc
    19561 tgtgccttat ccgccggaac aaattgtgta acaatttcta caccagaatc caacaatttt
    19621 ttatacgtgc tgatattttc atctgatgca taggctctag aagatagctg taatgcatta
    19681 tctttaacaa cagtaccgcc aacaattagt cggtcaattc taataccgtt atctactaaa
    19741 tgaaggattg tttccggctc tttcacgatg acaaatactt tttcctgatc gtatttcccc
    19801 tttttaaaat tcgcaatcgc tgtttcttca gtgataattg atacagccat tccagcaggt
    19861 ctagctaaac gcatacttga tttttggatg tcgttatttg cgacagaatc atcgatcacc
    19921 atcactctgt ttgctttaga cattggtgcc cattgcgtga caataatccc atgcaataaa
    19981 cggttatcaa ctctcgacat aacaatactc ataaatactt cctcctttat ctttcatttt
    20041 cttacaagca attaccatgc caaaacaaaa agaacttgat aaatcaagct ctttttacat
    20101 aatcataaac gtatttaacc tcatgaatcg ggatgtcgac actataataa tgttcaacga
    20161 cactgaaaga ttgcttaaaa tgtgtctgaa atttttgaaa ttcctcactc gtttcattga
    20221 aataaatttc atcttccaaa gtattttgtt ttactaatcg ttcaattaag caactaatgt
    20281 gaacatataa tccgaaacac gtattattat caaatgaaat tcctagatca ttttgtaaga
    20341 tgtatatagc atctgaaacc cgttctaata atttagtcgg atttaatatg gtcaattgct
    20401 ccattaaatt cgttagacta aaattcttca gaatattatt gttcaaaact ttcagttcat
    20461 tatcattaaa gtgtctattc aattgattgg agaatttact tatattcatt cctaaaatca
    20521 tatcttcaat tgaaaagaat tctacatctt ctagatgtgg atttagtgtt ccaacaataa
    20581 aaagaatatt gttctcttta cgaagctccg ataaatagtt ttcattcact aaagagtaat
    20641 agtcacacgt ttctatgaca agatcagtat cccttggaaa gctatcttca agtatctttt
    20701 ttaatttaaa cgaagttcct aatccgcttg cacaagaaca gacaatcata tccttcttaa
    20761 tatttgatga ttcataaata taatccacat tttgattttc ttttacttgt tcaagaatct
    20821 gtttaagcgg taaatctaat aatatctgat ttgcaacatc aatagccatt cttgttgaaa
    20881 cattgtttag tagtccgaat tttttttgag cgtctagttc caaatttaga taaattttct
    20941 ccagtgaacc catatcaaca agtaaaataa tttcttcata tccggtacac tcttgtaagt
    21001 atctattaat tttgtctgca atctcactac ttgaagtcat tagtggcata tcaatggcgt
    21061 cgtatatata tctctcaacg atcctattag cagtacttgc tatacaggtt gccgttagat
    21121 ctccatgagc caaaatcagt gctaaacgtt tttgctgaaa ttctttacta cacattaact
    21181 taaataaaat aaccaaaaca tttttttgaa tttttg
//
LOCUS       DAJSBN010000046        20504 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJSBN010000046 DAJSBN010000000
VERSION     DAJSBN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.046"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..703)
                     /locus_tag="ONT86_001998"
     CDS             complement(<1..703)
                     /locus_tag="ONT86_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2352764.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="ONT86_001999"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="ONT86_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="ONT86_002000"
     CDS             1758..2837
                     /locus_tag="ONT86_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR2352765.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="ONT86_002001"
     CDS             2869..3462
                     /locus_tag="ONT86_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2352766.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="ONT86_002002"
     CDS             complement(3572..3799)
                     /locus_tag="ONT86_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2352767.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="ONT86_002003"
     CDS             complement(3902..6490)
                     /locus_tag="ONT86_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCR2352768.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="ONT86_002004"
     CDS             complement(6510..7169)
                     /locus_tag="ONT86_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2352769.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ONT86_002005"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ONT86_002005"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR2352770.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="ONT86_002006"
     CDS             complement(7942..8808)
                     /locus_tag="ONT86_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2352771.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="ONT86_002007"
     CDS             8917..9558
                     /locus_tag="ONT86_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR2352772.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ONT86_002008"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ONT86_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCR2352773.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="ONT86_002009"
     CDS             complement(10999..11898)
                     /locus_tag="ONT86_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR2352774.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="ONT86_002010"
     CDS             complement(11900..12697)
                     /locus_tag="ONT86_002010"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCR2352775.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="ONT86_002011"
     CDS             complement(12675..13379)
                     /locus_tag="ONT86_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCR2352776.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="ONT86_002012"
     CDS             13510..14085
                     /locus_tag="ONT86_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCR2352777.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="ONT86_002013"
     CDS             14203..14880
                     /locus_tag="ONT86_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR2352778.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ONT86_002014"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ONT86_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCR2352779.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="ONT86_002015"
     CDS             complement(16943..17941)
                     /locus_tag="ONT86_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCR2352780.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="ONT86_002016"
     CDS             complement(18167..18820)
                     /locus_tag="ONT86_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCR2352781.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            19254..>20504
                     /locus_tag="ONT86_002017"
     CDS             19254..>20504
                     /locus_tag="ONT86_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2352782.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT         5953 a         4408 c         3586 g         6557 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagtcttt tggctctata
    19141 ctttttgatg ttggtgaact tcaccaacat cttttttgta taaaaaaata tagaaaagac
    19201 accctgaaat cctttagaat taatttggct accaattcat aggaggattc aagatgtctc
    19261 atactcagct tatcaaagat accctaaata ttctagacct aaatatctat ttcgaggaaa
    19321 attgtttaac taaagaaaaa tacaaagggc aaatttgctt gatttacaaa ggaactcttc
    19381 tgtacaagcc agaagagtgt tcccattgtc tttgtgtcgt cccctctagg atcattcgtt
    19441 gggggacgac aaccgttcgg ttgttactga atgatgtgtc tgagtatcgc acttatctgg
    19501 agctgaagaa acaacgattt aaatgtaagt cttgccaaag aacattcgta gcggatacct
    19561 ctgttgctga aaaacactgt ttcattagtc aaaaagttcg ttggtctgtc attgctcgct
    19621 taaaagagaa tacttccatg acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg
    19681 tctatcgtgt catgaagcga ttctaccgcc cattgaatcc tttcaaacag actttgccaa
    19741 aggtcttatg ctttgatgaa ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta
    19801 tcatgatgga cggacaaagc catgcattat tggatattgt cgaaaatcga cgcttgcctt
    19861 atttagaacg ctatttttct cggttttcat tagcgactcg tgaagccgtt caattgattg
    19921 ttattgatat gtatgcgcct tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt
    19981 tgatcatcga tcgtttccac atcgtccaac atattggcag aactttcttg aatcatcgag
    20041 tgaaagaaac cacggtacga cttaaaggaa attcccactc tcaacgaggg ttagggaaaa
    20101 aattgaagcg ctattggaaa ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac
    20161 gggtttggcg tgccagtttt aaagattatt tgactgaaag tgagattgcc gatcgactgt
    20221 tagctgcttg tcccaactta cgccaaggct atcagttata ccaagacatt ctttatgcgg
    20281 taaagaaaag agatcagtca ctatttgagg attgtttaac tagagacatt agcggtctgc
    20341 cagaaaccta tacaaccact ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg
    20401 cgcttcacta cagctattca aacggaccat tggaatgctt aaacaaccac atcaaagtat
    20461 taaaacgcaa tgcgtacggc tttcgcaact tttacaattt caaa
//
LOCUS       DAJSBN010000047        20488 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJSBN010000047 DAJSBN010000000
VERSION     DAJSBN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.047"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="ONT86_002018"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="ONT86_002018"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCR2352783.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="ONT86_002019"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="ONT86_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCR2352784.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="ONT86_002020"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="ONT86_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCR2352785.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="ONT86_002021"
     CDS             2857..3732
                     /locus_tag="ONT86_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCR2352786.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ONT86_002022"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ONT86_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR2352787.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="ONT86_002023"
     CDS             4629..5063
                     /locus_tag="ONT86_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR2352788.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="ONT86_002024"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="ONT86_002024"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR2352789.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="ONT86_002025"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="ONT86_002025"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR2352790.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="ONT86_002026"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="ONT86_002026"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR2352791.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="ONT86_002027"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="ONT86_002027"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCR2352792.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="ONT86_002028"
     CDS             13334..14260
                     /locus_tag="ONT86_002028"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCR2352793.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="ONT86_002029"
     CDS             14442..15335
                     /locus_tag="ONT86_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCR2352794.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="ONT86_002030"
     CDS             15469..15594
                     /locus_tag="ONT86_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2352795.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="ONT86_002031"
     CDS             complement(15728..16360)
                     /locus_tag="ONT86_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352796.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="ONT86_002032"
     CDS             complement(16508..17050)
                     /locus_tag="ONT86_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCR2352797.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="ONT86_002033"
     CDS             17185..17574
                     /locus_tag="ONT86_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352798.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="ONT86_002034"
     CDS             17578..18342
                     /locus_tag="ONT86_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2352799.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="ONT86_002035"
     CDS             18471..19418
                     /locus_tag="ONT86_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2352800.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="ONT86_002036"
     CDS             19548..20288
                     /locus_tag="ONT86_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352801.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7194 a         3481 c         4316 g         5497 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagtttat
//
LOCUS       DAJSBN010000048        20372 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJSBN010000048 DAJSBN010000000
VERSION     DAJSBN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.048"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..455)
                     /locus_tag="ONT86_002037"
     CDS             complement(<1..455)
                     /locus_tag="ONT86_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR2352802.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
     gene            complement(560..1423)
                     /locus_tag="ONT86_002038"
     CDS             complement(560..1423)
                     /locus_tag="ONT86_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR2352803.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1619..3718)
                     /locus_tag="ONT86_002039"
     CDS             complement(1619..3718)
                     /locus_tag="ONT86_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR2352804.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3938..5047)
                     /gene="rpoD"
                     /locus_tag="ONT86_002040"
     CDS             complement(3938..5047)
                     /gene="rpoD"
                     /locus_tag="ONT86_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR2352805.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5071..6912)
                     /locus_tag="ONT86_002041"
     CDS             complement(5071..6912)
                     /locus_tag="ONT86_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR2352806.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7094..9409)
                     /locus_tag="ONT86_002042"
     CDS             complement(7094..9409)
                     /locus_tag="ONT86_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2352807.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9594..10454)
                     /locus_tag="ONT86_002043"
     CDS             complement(9594..10454)
                     /locus_tag="ONT86_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCR2352808.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10513..11034)
                     /locus_tag="ONT86_002044"
     CDS             complement(10513..11034)
                     /locus_tag="ONT86_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HCR2352809.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11312..12073
                     /locus_tag="ONT86_002045"
     CDS             11312..12073
                     /locus_tag="ONT86_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2352810.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12099..14690
                     /gene="mprF"
                     /locus_tag="ONT86_002046"
     CDS             12099..14690
                     /gene="mprF"
                     /locus_tag="ONT86_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR2352811.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14878..16065)
                     /gene="tuf"
                     /locus_tag="ONT86_002047"
     CDS             complement(14878..16065)
                     /gene="tuf"
                     /locus_tag="ONT86_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR2352812.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16433..16789
                     /locus_tag="ONT86_002048"
     CDS             16433..16789
                     /locus_tag="ONT86_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2352813.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(17030..18034)
                     /locus_tag="ONT86_002049"
     CDS             complement(17030..18034)
                     /locus_tag="ONT86_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCR2352814.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            18200..19051
                     /locus_tag="ONT86_002050"
     CDS             18200..19051
                     /locus_tag="ONT86_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2352815.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            19068..19313
                     /locus_tag="ONT86_002051"
     CDS             19068..19313
                     /locus_tag="ONT86_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352816.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            19500..20204
                     /locus_tag="ONT86_002052"
     CDS             19500..20204
                     /locus_tag="ONT86_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352817.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         5759 a         4182 c         3565 g         6866 t
ORIGIN      
        1 ttttcttttt catagcattc ctgacagatt ccatggaacg ttagccgatg atcttttact
       61 aaaaaatgat aacgttcttc tactacttgc tccacttcaa gtaaaagatc ctcttctact
      121 tcctcgatcg tgccgcagtt caagcatagt aaatgatgat ggaaatgttt ggcgccttct
      181 tttctcaaat cataacgggc taatccatca ttaaaactta ctttatcgac aattttcaat
      241 tctgtcaaga tttcaagggt tcgataaaca gtagcaagtc caatttccgg actctttttt
      301 ttcactaaaa agaagatttc ttctgcagat aaatgctctt tctcattttc caaaagcaca
      361 agcagagtag cttctctttg cggtgtcagc ttaaaccccg cttcatgtag ctgctgtttt
      421 gtttttttca tcgccgaaat agcattcatg accatttact caaccccaac aatttataat
      481 aattacaaat aaaaaaagat ataacactat gttaattaaa attattataa gctagttaag
      541 aaaaacatgc aagggattct caattagatt ttttgactag ttttgtcaca ccgttttctt
      601 gtgtaatcaa tccttgtttc aaaagattac ctaatgcgcg cttaaatgct cctttgctga
      661 taccaaaagc tttcatgatt tcttctggat ccgatttatc tgtaaagttg atttgctgat
      721 ctgcagaacg ttctaaaatg gttaaaatca tttgtgcatc atctgatata gcttcatatg
      781 ctcttggctt caatgaaaga ttcagtacac cgtctggtcg gacaccaatc actcgacctt
      841 ttatctgctc gcctagacga ggttcattat atcgttcaga aggatggata aacccaagat
      901 agaaatcttc cgttaaaacg tacgtacctg ttaatttcaa gcggtatgct gttcctatca
      961 catctttgtt tttcatttct tctgttccat atttgcttaa agatttgaat attttttcat
     1021 ctgctaaact gccccaaata cggtcttttg catcaactct aagggaaatc atcaagcgat
     1081 cccctttttt aggccatagc tctgtcattg tcggcaattc atcaagagat acgacaaagt
     1141 ctttatctgg aagaccgata tcgacaaacg cacctaaatc ttttcgtgta gcagtaacga
     1201 ttccaaaagc gtaatgaccg atacggcttt taggaatttg tgtagtgatc cgattttcat
     1261 gtttttgatt ttggtaaccg aatccttcaa ctgcttcacc gattttgtgt tcaccttctg
     1321 atttgcttaa gccaaaggtt tgtccgtttt tttggacaaa gtagtttttt tcattttcat
     1381 caataataat acctgtaaat atagttgcta ataaatcgtt catttttttc tccctctata
     1441 gtgaaaggtt ttcttaatac atttctttgc tatcataaca taatgttatc tacttgaata
     1501 gctgagcctt ggtttggaaa ggtgaaaaaa ggagtatgac aacagttgtg tcatactccc
     1561 ctttttcgaa taaacggtgt taaacggcag gctcacagcc tctttcattt gcctaaaatt
     1621 aattgctccc atttcctccg aaccatccag ttttacagtt gatggttacg atgtaaaatg
     1681 gcaaagcact ttctccaatg cctttcatca atgcagattt tgttgaagtg gcatatagat
     1741 tataatagcc ttccccattt tcgattctgg ctttttctaa tctgtaacct ccttcaacga
     1801 tatatccacg ttggatacat gtagcaatca ctttgctttg gacacctggt gcccacgtcc
     1861 atgctggatt agatgcatcg gcaatatcac ttgctctggc agatttgatt ggctgattcg
     1921 ctgagtttga agaggaaggg gtcgaggatg ccgcttcttg ctgtgctgct tgtgcgaccg
     1981 cagcttgttg agcggcttga gctgcagctg ctgcttgagc ggcttgtttt gcttgttctt
     2041 cagctgcttt tttcgcttct tctgcttttt tcttttcctc ttcttctttt ttctctttat
     2101 cagtgagcat cttatctact tgtttcaatt gttcattcaa ctgagcagat agatctgtat
     2161 ttttcacttt ttctactgct tcttttgccg tcttgtattg gtcgcgtgtt gctgaatcca
     2221 ctacttttcc gtctttaaag actatagctg ttttttcttt agctgtttgc aattcagcat
     2281 attgattttt agcttctgtt tgtgcttgtt gaatcaattt tccaaaatct gtgttatccg
     2341 tttcgattgc ttggatctct ttatctgctt tcaacttgat gtcatctgct agatgatcat
     2401 cgacgatggc aggtgctatg aaccactcat tcactttttg gatatctcgg atcttagatt
     2461 ggatatcttg gtaagttgtt tctaactctg aatatccttt ttcattctta agactggata
     2521 aatcattttt tagttttgat aaatcttgat gaatcatatc tgcacgaata aattgatggt
     2581 gatcatctgt ataaaaggca gcaagtttct catgaagttc ttccatttgt tgctgtcttt
     2641 ttgcttctgc tgctacttcg gcttctactt tttgatgatg gtcataataa gcccagcttc
     2701 ctccaccgag tagaagtact gctgctaaaa cgagataagg tacttttttt gattttttct
     2761 tttgcgcttt atctggatct gttttttcta agtttgattc ttgatgtgtt ttttcagtat
     2821 ctggcacgat tgttattgat ttcgcttctg cttcaaacag cttaggctgg ttctcttctt
     2881 tttctatttt tgcttcaaga tcagtaccct ctttgcttac agattgattt tcagcatcgg
     2941 attcactatt tatttcatcg ggctctgttg actttttgtc tgtgttttcg agtatttctt
     3001 ttttctctgc ttccggcagt ttattcgttt tttccttttc taattgtttt tcagtaaata
     3061 cttcagtttt ttctgtgata tccgttttta cgtcttttat ttttactgtt tctttacctg
     3121 tggcgttttc tctatcagag tctttttcag tttcttgttc cttttctggc tctactttgt
     3181 tttcctgtgc aattttttca ctgatcattt tgagcagctc tttcgcgtgc tcttcttttg
     3241 tatcttcacg atgtgctttg atataagcgg atagtatcga attttcttcc tcattatcga
     3301 cgatttcttt cagttcagct actcttcttt tttgttttga ttcaagcgtt tcttcttgat
     3361 tctttcctga ttcttccgaa gtctcatgaa cagttgcttt cttctgttca tttttctcta
     3421 tattagttac ttgatcgtga gcaggtggtt cttcgtcttc tggttctgct ttttcgaata
     3481 attccattac agattctaga ggtaaatctt tcaattccga ccattcgatt ggatcgttta
     3541 attctgtatc aggaaagact gcatcttcag ttgatttatc ctgcatagct gcatctgtat
     3601 ttgtttcttc aattgattct tttatttgct gatcttctgc agttatttga tgaccgcagt
     3661 ctggacagat agtattctgc tccatttttt tcgatccgca atttggacat tttttcatca
     3721 cttagcgatc ggctccttta ctaggtttac gctaccattt aatctatcat attctatacg
     3781 tcacgccaat agcttatttt taggatttac ttaaaaataa tgaatgaatt tccttcctat
     3841 agaaaaaaaa gcttgagact gcgtagtctc aagctttttt tcttttaaaa taaaccaaac
     3901 agttttactg atttctcttc tggtcaatag tccacgctta ttctaagaaa tcttttaatt
     3961 gttttgaacg agaaggatgg cgcaattttc ttaatgcttt agcctcgatc tgacgaatac
     4021 gttcccgagt aacaccaaat actttcccta cttcttccaa tgttctcgtc cggccatcat
     4081 ctaaaccaaa acgtaagcga aggacattct cttcccgatc agtcaatgta tctagtacat
     4141 cttccaattg ttcttttagt aactcataag ctgcgtgttc tgctggactt gttgcttcct
     4201 ggtcttctat gaaatcacct aagtgagagt catcttcttc accgattggc gtttcaaggg
     4261 aaacaggttc ttgcgcgatt ttcaagattt ctcggacttt ttctgttgga agatccattt
     4321 ccgcaccgat ttcttctggc gtaggttctc ttccaaggtc ttgcagcaat tgacgttgga
     4381 tacgtatcaa tttattaatt gtttcaacca tatgaactgg aatacggatc gtacgtgctt
     4441 ggtcagcgat tgctcgagtg atggcttgac gaatccacca tgtagcatag gttgaaaact
     4501 taaatccttt gcggtagtca aatttttcaa ctgccttcat cagtcccatg tttccttctt
     4561 ggatcaagtc aaggaactgc atgcctcgtc ctacataacg tttagcgata cttactacta
     4621 aacgaaggtt tgcttctgcc agacgctgtt tcgcttcttg gtcgccttct tcgattttca
     4681 atgctaacgc tacttcttct tccgcagtta aaagggagac acggccaatt tctttcagat
     4741 acatacgaac cgggtcattg atttttacac cggttggcgc agaaagatct tctgtttttg
     4801 cttgttcagc ttttttctca gcactcttca agctgtgaat tgatggatca ccattttcat
     4861 ccaccacact gatacctgcg tcttcaatct tttggatcaa tttgtccatt tcatctgcat
     4921 tcaaagtgaa tggtgtagca agctgattgg ataaatcatc atatacaact tgtcgtttgg
     4981 gtttgttttc tttgatgaaa gctgctactg ctgcttcata cgttttttta tctgttccat
     5041 ttgccataaa agaaggcccc ccttgagtag ctatttggct tgttttaact gtttcgttaa
     5101 attgatgatt tcaatcgtca attcaagctc aagctgtttg tttccagttc gtctggcttc
     5161 ttgctgcatt tgttttttta atgttatttc atcagctaag ccagatttgc tgatcacatc
     5221 tagcaagtct tggatttctc gtggcgaact ttcttctgcc accggtaaat attcgatttc
     5281 caccaccaac cgccttaaat tttcatcttg caaaaagtct aaaaactctg caaggttgaa
     5341 ttcataatgt tgtgagacat atgcatcgaa taaaaaataa acttctgcat atgcatcatg
     5401 aataaactga aattctcgtt cttttaaaat attacggaca gaaacttctt taaatgcacg
     5461 atataataga atctgctctg ccttttctac ttgcgtcaaa ggtctctttt tccgtgcagt
     5521 ttcttgtctc gtaacctgag tgtgttgact ttcctgaggt tcttgacgat tttgctgatt
     5581 tgtccttcga agatcgcgca gttgtttttg tatcgtctct ctgccgatct gaaactctgt
     5641 tgacagttga gatagataca tatcttgttc gattggtgaa acaactcggc taagttcaac
     5701 tagtaattct tcgaggtatt cgatcttgtc tttttcatta tctaaatttt tcccttgttt
     5761 taaataacgt gatttaaaag taaagactgt ttcgcgccca tgagccacta attcttggaa
     5821 agatgcttct ccatacttac ggagatattc atcaggatca agcttttctg gaacgactac
     5881 gacagaaagt gctaatcgac tgttttgccg aagcaattca atccctcgat tggttgcttc
     5941 aaaacctgcg ttgtctccgt catagcaaaa gaccaattct ttcgtcagct tttccatttg
     6001 ttggatctgt tgagctgtta gacttgtccc catagaagct aatccagttt tgataccagc
     6061 ctgccatgca gcaatcacat ccataaaccc ttcgaacaac aaaatttctc cgtttttccg
     6121 gatatcgctt cttgctttat caagattaaa taaaacttgt cttttattga acagttctgt
     6181 ttctggacta ttcaagtatt tgggctgatc tttttcctgc cctttttcag gtgcaagcca
     6241 gcgtcctgaa aaaccgatcg tctggccttg agaattacga agtggaaaca tcactctttg
     6301 ataaaaacga tcagacatac ggccatcatc atgcgtgaca aacaatccag agcgttctaa
     6361 caattcattt gaaattgatt cattcttgaa tacttgctga agaaaagaac gctcattagg
     6421 tgcaaaacct atgttgaatt cttcaattaa ttctaaagac aacccacgac tttgcaaata
     6481 ttctaaagct ggctgtccag cttttgtatg caatagcatg tgatgataga tttccgctgc
     6541 tttttcatga gcagcaatca gtttccccgt agcagattca ggattttgga cagctatagt
     6601 acgctgacgc cattgatctt ctaacggtat ctgttcgaag tctgccactt ttaccactgc
     6661 ttctggaaaa cttagacctt ctagttcttg aagaaaagta aacacgtttc cgcctttacc
     6721 acaaccaaag caatgaaaaa tctgcttgtc ttctgcaaca gaaaaagacg gtgtacgttc
     6781 ttcatgaaaa ggacacagcc ccaagtaatt tttaccagac tttttcaact ggacatattg
     6841 tccaatcacc tcaacgatat tcgtttgact gcgtactgtt tcgataacct cttgaggaat
     6901 ccgttgagcc attttttcac cccctgctct attgatatct tttgggttta acagaagata
     6961 tttcaggaga ataaccggta aaaaatcgca ctaactgaaa tcagtgcgat tttaaaggac
     7021 acaattatac atgtttgatt gtatcatatt tcattgcttt ttcaagtata acacatttct
     7081 atctcattaa gcatcaattt gctactcttt tcagtaactg attttgaata tcagccttcc
     7141 agtacaatac tcgaagaaca atatttacga gcatgaatac aaagaagtat cctacgctct
     7201 ctcccaaccc taagtttact tccaaaattc catggaacag cattgcagct acatatatcc
     7261 ctacgatcgt cgttactgct aggaagatgc gcaatgggtt gaagggataa caagctttta
     7321 caactgccat gcaactgatc ccaatcagca agtaatacat caaagtagta gtttcagcat
     7381 ttccccatgc gaatgttttt ccaagtagat acaccgcaat gatattcact actacaagca
     7441 aggcatttgg taaagcatta ataagtgctg taggcaaata acggtaagtg atttttcttt
     7501 tatcttgttc aaaagataaa aagaaggaag gatacccttc aatcgctaaa tcaatcagcg
     7561 tgatttggat agggataaac ggaaatccaa ttgctgtaac ggcacatata atcgataaaa
     7621 taaatgaata aatcgttttg ataaaaaaga tgcctgagac tttcgtcaca ttgttgacca
     7681 ctcggcgtcc ttcaaacagg acttctggca atgtagtaaa atcagagtcc aacaatacca
     7741 gattcgcaat ctgacgtgtt gctccatcac cttctgccat agcaatgctg caatctgctt
     7801 ctcttaacgc caaaacatca ttcacaccgt cgcctgtcat tgcaaccgtt ctgccgcttt
     7861 ctttcaattc atttaccagc aatttctttt gctgcggact cactcgtcca aaaaccgtat
     7921 agcgatgagc agcttctcgc acttccattt cttcagtaat ttgagaaaga tcgacatacg
     7981 catcataatt cggtaatcct gcacgtctag cgatattgga gacagtaaaa ggattgtctc
     8041 cagaaatcac tttcagatcc acaccttcct catgaagata agctagtgtt tcttgagcgt
     8101 tttttcggat tggatcatcg atttcaaaca gagcaaccgg ctcaacaaat gctggttttt
     8161 cttcgttcaa ttgctgattt ggagcgatcc ccaacataag tacgcgatag ccattcgctt
     8221 gggctgtttt tagactgtcc ggaagccgat cttcttccac caaacgttca ggcgctccta
     8281 cgtacacgac tcccagttct ggaaattcca tcgcgcccca tttgcgatca gaagaaaatg
     8341 cgactttgtt tgtcacatca taccgattag aaagctcaaa ataatctctc atagcccgca
     8401 tagtgatgtt gttatcactg ctttctgcca gatacgtccc cataatttct gggatggctt
     8461 tctcgtacag cggatgtagt gcttctaccc gctggatact cattttccct tcagtaatgg
     8521 tccctgtttt atccaagcat aatgtatcga catgggccag cgtttcaatt gaatacatgt
     8581 cttgaacaag gattcgtttt tttgccagtt tggttacccc tgtcgttaat gcaatactta
     8641 ttagcaatac caaacccttt ggaagcatgc ctaacaaagc tgctgcagat gcaataacag
     8701 aaattttgat agaagcatcg cggatgaaca gtgcttccaa aaatagtatg atccccaaag
     8761 gaataatcac gaaactagtg aatttggata ctttgcgaat cgaatctaca agttcagaac
     8821 gaatagcttt gtgagtcttt gcctcgctag tcaagcggac agcataatta tctgcaccta
     8881 ctcggataac ttcagccatg acagaaccgc ttgtcaaaaa gcttccagaa agcaattcag
     8941 ctccagtttc tttgacaatc aaatcagatt cgcctgtcaa aagagcttca tttgcttctg
     9001 cccgtccaga tataacctgt aaatcggctg gtatctgttc tcctgctccg atttttacca
     9061 aatctcctaa taccagctct ttagtagaaa tcgattgttc tttaccgtcc cggatcacta
     9121 gtacttctga ttgggaaaca atcgataatt tccgtacaag atttcttgca tgtatttctt
     9181 gataaattcc aataacgata ttgacgacaa taattgctaa atacgccatg ttggaatacg
     9241 cacctacaaa taacagacaa atcccaatac caaagttcag aaaattgaat aacgtcaata
     9301 tattatcgct gaatatttgt tttgtacttt ttgatgcatc ttcctggtag tcattttgct
     9361 ggccagaagc cattttctga actacctctt gctgtgtaag tccattcatt ttctttttct
     9421 ccattttccg ccaactttca aaaataatca cgtgtttaag tctagcgtgt tccaagatta
     9481 aaaacaactt gtttttataa aatttataga aaaacacacg aatgatttta caaaaaagat
     9541 gccaaccttt ttgtttgaat cacccgtgtg ctgtttttat tatcaatcta tctttactta
     9601 tattgttcct ccaaacggct catccaccaa cctagtgctg taccggctat aaaaagaaca
     9661 ggacaagcta gtaccccagc aactcccaat cccgcgtaat cggtaggaat gatcataatc
     9721 gttgaagcaa aaactacacc taaaatgaaa tggaatagct tgctatatgc ctttgagaaa
     9781 atgtaatcca tgatttttga caatccaaga acacaaagaa tcccaccaat tccgatcggc
     9841 acaatcactt caagtcgtac ttctttaaat ccgtcagcca tcgctttata caatcccata
     9901 tacaccaaaa aattagaagg gctcaatcct gggacgatca tacccaaacc aatcaatccg
     9961 ccggcaatca gccatgttcc aaaattctga tcgacttgcc cgtcaaataa ttgagcgccg
    10021 tataataaaa acaaataagc cagaacaaaa gtaacaacca tgatgatcac atcagaagta
    10081 tctcttcctt ttttccctgc ttctttccat aacgcaggaa cagttccagc gatacatccg
    10141 acaaaaaacc ataatatcgt tgtttcatac gcacctaata aaaaactgac taaaaaagaa
    10201 agtagaaaga tgcctgtcac tccgcctaga ccaacaggca gaaaaaacaa aacattttct
    10261 ttgaaatttt ttgtaaggtg ggctaaaaaa gaaatcagcc gctcatacat gccaaagaca
    10321 gctgctaatg caccaccgct gactcctggc aagataaatc ctgaaccgat aaacattccc
    10381 ttcacaaacc gtaacatcca gtctttggtt gtgtacccca cttcttgatt ttccattttt
    10441 tgcctttttt ccactatttg cgcactcctt ctttcattta attttattca tgattgaaca
    10501 tttcctgcta ttttacttaa agactttttc ataggtctct attatttttt cattcatttt
    10561 gacaatatca ggataaaaca tgttacctcc attatgatag agatagccgc cattgtaaag
    10621 caaggactgg atccgccaat atcggtattg tttcccttcc tcatttccta aaagtgggct
    10681 cagcacatct ttagaatacg cttctgctaa ttctactgtg ttttctccgc catgattttt
    10741 tacataggca atataatctt tcccaaaatt atatgcttgg acagctgtcc ataaatcgca
    10801 tccttgtttc tttgcttcta ctatcatttc cgccaaatat ttgacacctt gatcaatact
    10861 gtcagctgga ttatcaatga tctcagcttc tccatgcaga ctttccgagc tctgcatgag
    10921 atctgtccct tttccttttg attcggtcaa tattatcgcc ttgatcaaat tctcgtagtt
    10981 ttccatatga taattttttg cagctgttgt tatttctatt tgatagcttt ccactcgttt
    11041 taccgaacga taagttcttc ctcataaaaa caacaccgca ccaataaccg tcagaatgat
    11101 catgtaaaaa agaatacgga taatagataa tcgttttcgc ttcatttcat cacctagtta
    11161 ttttgacatc tttttttatc ttactatttc tttatcttct tggctatttc ttccttgatt
    11221 tctgtgtacg ccttaagaaa aaatgaattt tcctgtattt tttgacatct tacgaaaatg
    11281 atcgttgttt ttcagtaaaa acaattgccc aatgactgat ataatcgtta tactttacac
    11341 agtaggtgat tatgtgaaaa aagaaaagaa atatgcaaaa atgccagatg ggagcgaaat
    11401 ttactatgaa aagagcggtc aaggcttccc tctcttttta ctgcatggca atgacggcag
    11461 cggccgcttt ttttcagaac aggtccctgt actcgaacgc tactataccg tgtatctagt
    11521 agatagccga ggacatggtc gttcgacaaa cgaagcatct atgttgaatt ttcagttaat
    11581 ggccgaggat ctgaatacga tcatgttgct ggaaaaaatc gatcaggcag attttcttgg
    11641 atttagtgat ggagcaaatc tagcactggt atttgccagc agcttcccta aaaaagttca
    11701 tcgattgatt ttaaattctg gaaacacgct tgttaaaggc gttcgttttt ctgccagagt
    11761 catcagtaat attcattatg catgggtatg gttactctct ttatttcgcc caagtcttcg
    11821 caaaaatctt ttagttatca aacttttgct gcatgacatc gggcttacag aaaatgattt
    11881 aaaaaaaatc aactccccca ctttgatcat cgtgggaaaa aaagatgtaa tcaagctaaa
    11941 acattctttg tatatagcta aaactatacc aaaagcttcc tttgtgcttg tgaaagaaca
    12001 aggacatgaa ttagctagaa aagatcctga acgcttcaat cgagaagttc ttcaatttct
    12061 ttctgaaaca taactattag gaatagaagg tgatttattt gttaaaaaat accatacaat
    12121 ggctgaaaaa ccacctagga ctgttcaaaa caatcttttt gatttctgtc attgtcatta
    12181 ttgtcggtga attgatgtcc atcggtaaaa ctctatctat ccagcaatta ggagaaacgt
    12241 ttgccactat acctgtatgg aaaagcggac taatgctatt gattggtctt atctctgtgt
    12301 tacctatggt tggttatgat attattttga atcggatgct tgaacaaaag cagaatcctc
    12361 gttacctgtt tgagacaagc tggctgatca atacgatcaa caatattgcc ggtttcggcg
    12421 ggtttgttag tgtggggctt cgctctgaac tatatggaca aaagaaagag agcagaaacg
    12481 tgatccaagc attatcgaaa gttttcttgt ttttgatggc tggactttct atttacagtc
    12541 tgctttcttt tttacttgta gtcttcacgc cagttgctcc ttttttgaag caatattgga
    12601 tatggttgat cggcggcggt ctatattttc ctgtcgtctt tttatttact actttcaaaa
    12661 agaaaggatt ccttggtggt acctcactta aaactcaagg acagctattg ctagtttcat
    12721 tgttggaatg gaccggtgtc ctgtttagtt tcctttctgt tggttattta atggaaatcc
    12781 ggattgatct gtggcaaaca attccattat tcattgctgc ttcagtgata ggaatcgtgt
    12841 ctatgattcc aggagaaatt gggagtttcg atgtaatgat gattatcggg ttgtccgcta
    12901 ttggtgtgcc aagagaaata gtcgttgtgt ggatcttgct ctatcgcctg ttctactaca
    12961 tcattccgtt tttgattgga atcgtctttt tcttcaaaaa tatcggttcc acttttgatc
    13021 agcgttattc tggtatccct aaacaattag caacagaaat tgcgcataaa atcgtggttg
    13081 tcttattata cttttctgga atcatgctag tactttctgc cactatcccg caagctttta
    13141 cagagtttcg ctggcttcat agtttgaacc cattgaaatt tcattttatt atccaatttc
    13201 ctagtatact attgggattt cttttaatca ttatgggcag agggatcgca gcacgtgtga
    13261 aacgagcata tttgcctaca atcttcttga ttgttctagc tttactttat gttttattaa
    13321 gtgattttag tttcacaccg gtggttttct tgtctattct gcttctgatc attcttgctt
    13381 ctaaaaacga attatttcgg gaacaattga tctattcatg ggagtggcgt acgatcgatg
    13441 gaatcattat tggtgcactg tccttgcttt acatcgttat tggggtatac aatctgccgg
    13501 atttccctca tcggcgtcac cattttattt cctttttctt atttccttct gaaaagattt
    13561 ggttttcggg attgttggcc atcatcgctg ttagtttcat gatcgttcta tttgttcatt
    13621 tccttcaagg agaaaagaaa cagattggcg aagcctttaa tgaagaaaag gccctgaaga
    13681 tccttacaac ttatggagga aactccgata gtcaattgat cttcttaaaa gataagcgga
    13741 tgtttgccta tgaaaaagac ggtgaaccaa ctgttcttct ccaattcgct tgcttcaata
    13801 ataaatgtat tgtaatgggt gatccttctg gtaagaaaga agattttcca gaagcaatcg
    13861 aagcttttat cgaagaaaca gatcgactat gctatttacc agttttctac gaaacaagcg
    13921 aagagattgt tatgattttg catgaattcg gctacgattt cattaagatg ggggaagaag
    13981 cttatgtaga cctgaatagt tttacaactt cagggaaaaa aatgaagggc actcgtgctg
    14041 tcttgaatcg gatcgaacga gaaggattta catttgatgt cttacaacca ccattttcta
    14101 ctgagcaaat gagtactttc aaaaatatct ctgataattg gttgggttca cgaaaggaaa
    14161 aagggttttc tttaggcttt ttctctgaag attatctaca aagagcgcct attgcagttg
    14221 tcaaaaatac ccaagaagaa attgtcgctt ttgcaacgat catgcctact tacacgaaca
    14281 ataaagtagg aacgattgat ctgatgcgat atgattctaa aaaagcacct tctggcagta
    14341 tggatttctt gttcttgcat ttgtttgcct atatgaaaga agaacatatc caatggttca
    14401 atttagggat ggcccctctt tcaaatgtcg gaacttccag aaagagtttt ttacaggaaa
    14461 ggattgcata cttggtttac gagtttggtt ctcattttta ttctttccat ggattaagag
    14521 aatataaaag caagtacgct acaaactggg tctcacgtta tactctatat tctagagata
    14581 gttggattgc atatgttatg attgctttgc tgattattga taatgcgcca gttgagcaga
    14641 cacaaggtag aataagtggt ttgaaaaaat ggatacgaag aaagtattaa aaactgcata
    14701 ccgatagatg aacactctca tgatgaataa aagagactga agtaaaacga aagttcatgc
    14761 ttctacaatc tcttcttata caattgaaga acaaagagcg gtccaaacag aaatcctgtt
    14821 tggaccgctc ttttatttta aacatttttc attaccaaaa tattcgagtc gcgtttctta
    14881 tgcttcgatc tctgtaacga tacctgaacc tactgtccgt ccgccttcac gaattgagaa
    14941 tgtcgttccg ttttcaacag cgattggatg gatcaagtca acatcaatcg tcacgttgtc
    15001 accaggcatg accatttctg tatcttctgg caatgtgatt gttcctgtaa catcagttgt
    15061 gcggaagtag aactgtggac gatagttgtt gaagaatgga gtatgacgtc caccttcttc
    15121 ttttgtcaac acgtacactt ctgctttgaa ttttgtatgt ggagtaatgg aacctggttt
    15181 agcaagtact tgtccacgtt cgatatcgtc acgttggatc ccccgtaaca acacgcctac
    15241 gttatcccca gcttccccgt aatctaacgt tttacggaac atttctacac ctgttacaac
    15301 tgctttttgt gtttcaggtt tgatccctac gatctctacc tcatcaccga cacgaacagc
    15361 accacgatca atacgacctg aggcaacagt tcctcgacct gtaattgaga agacgtcttc
    15421 cactggcaat agtaatggtt tatctgtatc acgttctggt gttgggatat attcatctac
    15481 tgtatccatc aattccataa tagcagcttc agcatctgga tcgccttgca atgcttttaa
    15541 tgcggaacct ttgatcacag gagtgtcatc gcctgggaaa ccatattcgc tcaataactc
    15601 gcgaacttcc atttctacca aatcgatcaa ttcttcatca tcgactaaat caactttgtt
    15661 caagaaaaca atcaggtatt ttacaccaac ttggcgtgac aataaaatgt gttcacgtgt
    15721 ttgaggcatc ggaccgtcag ttgcagaaac aactaagatt gcaccatcca tttgtgctgc
    15781 ccctgtaatc atgtttttca catagtccgc gtgtcctggt gcatcaatat gcgcgtagtg
    15841 gcgtttttct gtttcatatt ctacgtgtgc ggtattgatc gtaatcccac gttcacgttc
    15901 ttctggagct gcatcaatac tagcatagtc ttgaggattt gctaggcctt ttttgcctaa
    15961 tactgtagtg atcgcagcag ttaaagtagt tttcccatgg tcaacgtgtc caatcgtacc
    16021 aatgttaacg tgtggtttac ttctgtcata atgttctttt gccataattt acaaacctcc
    16081 taaataatat agatcactga aaaatttcag ctctcacctt tttattatag tctttttttg
    16141 actaaatcaa aaagaatacg tccgacttac tttttgtaag attcgaacta agcttattat
    16201 acgtcaatat tggtcaaagt caactaagag cactcgtctg tcttatacgc gaaaaaagta
    16261 ccttttataa ataaaaaaat gtgttttttg agaaaaactt caagatagtc cttaactttc
    16321 ttttgaagca ataagaacaa aatgaggaaa agtttaaaat tcagctgttg ccaacaactt
    16381 tcaatagacg aaatcttcta tttcgttaat aataaggaaa gaggtgaagt acatgaaaaa
    16441 attaattggc gtacttgtcg ttttaggcat atttctcggc ggaagttttg cagcttatca
    16501 ttatttttac ggcggcgaag cttattatac aaaaatcacg acgaatggcg aaatgtcagc
    16561 tacccaagca gataacggag agaaattcac gacttatacc tacaagcaag cggcttataa
    16621 taaagatggt gaaaaaaaac aggtgacgct tcgcgaagaa agagaacatc cattaaagat
    16681 gaatgcgtat cttaaactca aagtcaatcc tcgtaaaggt gtattaagct gggaagaaat
    16741 aaaggcttct gaagtgccta aaaatgcggc agaaaaaatc gataattagt aaatggtctt
    16801 aaaattttat tagataaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    16861 gagttgcttc tgcttccgcc gtttatacgt gtttagagcg tgagacaaaa gtgttcttta
    16921 cttttgtctc acgctctttt tttgttatat tcgtcttttc cgatccttct cttgacacaa
    16981 ttcatttaac tgatcactgg tacttgaaca cctaatattc tagtcatttt tataaacgat
    17041 tcaatacttc atccacattt ttgatcatca cggatatcgt accatccgga ttattcacaa
    17101 attcaatcaa atctgggttg cgatatacat ctactggtac gatcagttcg atcccgtttg
    17161 atagcttgaa tttttgtttt ccaaattttt tctcagaaat ttcttgtact tcttttacag
    17221 gaggtgcttc tgggacaaat ccttgctctt gtacttcttc tttaaaagcc atttttgccg
    17281 tgatgttttc cttgaagact ttttccgcaa tcatctcatg atccaaccga cctttttctt
    17341 ctatcgtatc atagacagct tctttcaaat cagccatgat atcaaattct tctgtctcaa
    17401 atttttttcc aagatgcttg gctacttttt ggatcacttt tacattttct tctaaagaag
    17461 gtgcaggaac actttcgatc actcttccag aaaaataaag ctcttttttg ccagaaaact
    17521 catatttttt ttcaattaat tcgtaggtcc aatcagtcaa attcacagcg aatgcttcat
    17581 ctgctttttg tgttttcgcc cctagaattg ctcgattcaa aatcaagcgg ttgtcgatcc
    17641 ctgcttcgtc tgcttcaaca aagtgagtgt aagcctcacg ataattgact ttcaataaag
    17701 ataagtacat cactgtatct aattcataca agacaacaaa tacatctgca ctaggtgctt
    17761 cttcactttc ttggtagata tcgaaccacc gctgaacgat tgcttcactt gcttccacaa
    17821 aattttcttg agcgatttta agtgattcaa cgatcatgct ttcttctgct agttgtccag
    17881 tttttgtttg agcagaagag attttttgga tcttcttttg aagaaaatca cgaatatatt
    17941 cttttgtcag atccagctct ttttgtgaaa agactggaga accagtttct ctgtcaataa
    18001 tatgcagaat tgcttttttt aaatagatat ccattttctc attcccttct gtcttagttt
    18061 acggaaaaaa aatcaaaaat accagaatca aaatcatttt cttttaaaca aaaatttcaa
    18121 gagtagaaac aaaatggatt ctttttttcg aaaaatgcgc tacacttata gttgaacgaa
    18181 taaaaaagga gctgaaagta tgaattcatt aacaagcact tatcgtctat caaatggtta
    18241 cgaaatcccg gtagtaggat tcggcacatg gcaaacacct gatggggatg tcgctgtttc
    18301 ctctgttaaa gaagcgctgg ctgcaggtta tcgccatatc gatactgccc aaggatacaa
    18361 gaatgaagaa agcgtcggcc aagcgattaa ggaaagtgga attcctagag aagagatatt
    18421 cttgacaaca aaattatgga atgcaaacca cagctatgaa cttgtgatgt cttcttttga
    18481 agaatcattg aagaagctcc aaacagatta tttagatctg tttttgatcc actggccaaa
    18541 ccctgttgct tttcgtgata attgggaaca agcaaatgca gatacatggc gagcatttga
    18601 agaactgtat gaagcaggta aaatcaaagc aatcggtgtg agcaatttct taccccacca
    18661 tctagataca ctagcaaaaa cagcaaaaat tatgccgatg gtcaatcaag ttttccttgc
    18721 accaggcgaa ttacagacaa ctgtagttga gtatgcaaaa aaacatgata tgatcctcga
    18781 agcatatagt ccattaggca ctggtaaaat cttcgatgtg cctgaaatga aacaaatcgc
    18841 tgaagcacat gacaagacga ttgcacaagt tgctttgcgt tggtcgctgc aacacgaatt
    18901 ccttcctctt cctaagtcag tgacaccaag cagaatcaaa gaaaatactg aattatttga
    18961 ttttgaatta acagaagaag aaatgaaaca aatcgatcag ttagatggtg tagtcggcaa
    19021 agcaaaagac ccagatacta cccaatttta agtcgaaagg tgattttatg gatccaatcg
    19081 aacgcttgaa ttctctctcg gaagaagtta cccagacttt tcattccgat tttgtctttt
    19141 tgatcgatgc agagaaaatc caacattttc ctgcaagaaa ctggacacat gatcaaatca
    19201 tcgaagaact gaaaaaacgg ttcgatcatt cgctaatggt caaaccctgg cacgaacatg
    19261 aagtcattta ttcacctgaa ctacctgttt ttgccttgat accgaaaaaa tagaaagaat
    19321 ggaacggatt atgtatatat acataatccg ttccattttt tatttttctt tcaaaaaacg
    19381 ggaaaatacg caaatttttc tgttgtaatt tgcgtatacg caagtttttt tgcattactc
    19441 cttttcattt ggctgttttt ttcttataat cgaaagggta tgagcgaagg agagaacata
    19501 tgaagaaaaa acagaaacaa aaattattaa atcaattccg tccttcattg gatggtgtaa
    19561 gaggacaatt attccgttct ctagaagaag agagcttgcg acgttatggt ttgccgttac
    19621 aagtcatgct tgatcccaaa aaccgtcaaa tcttaacgat cggattagct gggaaaacaa
    19681 atgaagatac acggatcaac gtgccaatcg atgacaactt caatactgta ttaaagagaa
    19741 ttcgttcagg agaaaacggg attttcgaac gattccgtga caatcttctt attgaaatcg
    19801 tgtcttactg gaatgatcaa cgattgaaaa acgatgaacc aactgcgcaa gctgttgcga
    19861 caccagttac agaagaaaca gtcagtgtgc cagaagaaaa aacggaaaaa ccaaaagcgg
    19921 acacaactga aaatacacgc tcaactgata cgaatgccgc tttaagtttt gctgattttt
    19981 caaaagccgt tgctgagtat cctaaatttt acacagaaca aaatgaacat gaagtagtta
    20041 ttaatgaaaa aaatgctgat gaacctcgta aattagcaac tatttctatg actactgaaa
    20101 atgagttcgt tatcgaaaaa gcactcgaac ggaaatacaa agtcaaattg actttgattc
    20161 ctttgatcga acaatttgca gcgacagcga ttactgatcg ataaaaattc tatttgtcat
    20221 aaaaggctgg gatttaaaat cccagccttt ttagtcatat aataatttct acagcaagta
    20281 aaaagaaatt ccttctaaca tcaggctcaa atatcagctt gacctgtcca acaaatcacc
    20341 tatgatccta caatgttagg cgacttctct tc
//
LOCUS       DAJSBN010000049        20155 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJSBN010000049 DAJSBN010000000
VERSION     DAJSBN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.049"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..152
                     /locus_tag="ONT86_002053"
     CDS             <1..152
                     /locus_tag="ONT86_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2352818.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="ONT86_002054"
     CDS             complement(230..763)
                     /locus_tag="ONT86_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCR2352819.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ONT86_002055"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ONT86_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR2352820.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ONT86_002056"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ONT86_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR2352821.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="ONT86_002057"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="ONT86_002057"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR2352822.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="ONT86_002058"
     CDS             complement(4488..5066)
                     /locus_tag="ONT86_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCR2352823.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="ONT86_002059"
     CDS             5388..5858
                     /locus_tag="ONT86_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCR2352824.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="ONT86_002060"
     CDS             complement(5966..7192)
                     /locus_tag="ONT86_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR2352825.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="ONT86_002061"
     CDS             complement(7946..8146)
                     /locus_tag="ONT86_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2352826.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="ONT86_002062"
     CDS             complement(8280..8888)
                     /locus_tag="ONT86_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCR2352827.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="ONT86_002063"
     CDS             complement(8973..9443)
                     /locus_tag="ONT86_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR2352828.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="ONT86_002064"
     CDS             complement(9491..10405)
                     /locus_tag="ONT86_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR2352829.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="ONT86_002065"
     CDS             10551..11354
                     /locus_tag="ONT86_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCR2352830.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="ONT86_002066"
     CDS             11532..12479
                     /locus_tag="ONT86_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR2352831.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="ONT86_002067"
     CDS             complement(12547..12840)
                     /locus_tag="ONT86_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCR2352832.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="ONT86_002068"
     CDS             complement(12868..13533)
                     /locus_tag="ONT86_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCR2352833.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="ONT86_002069"
     CDS             complement(13673..14305)
                     /locus_tag="ONT86_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2352834.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="ONT86_002070"
     CDS             complement(14312..15379)
                     /locus_tag="ONT86_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2352835.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="ONT86_002071"
     CDS             complement(15376..16107)
                     /locus_tag="ONT86_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCR2352836.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ONT86_002072"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ONT86_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR2352837.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ONT86_002073"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ONT86_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCR2352838.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="ONT86_002074"
     CDS             18270..19439
                     /locus_tag="ONT86_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR2352839.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..>20155)
                     /locus_tag="ONT86_002075"
     CDS             complement(19706..>20155)
                     /locus_tag="ONT86_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352840.1"
                     /translation="MNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTED
                     GQVIYSSFSPNKDEIIFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTV
                     KSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         6100 a         4019 c         3207 g         6829 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatataa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcat
//
LOCUS       DAJSBN010000050        19821 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJSBN010000050 DAJSBN010000000
VERSION     DAJSBN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.050"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            6..305
                     /locus_tag="ONT86_002076"
     CDS             6..305
                     /locus_tag="ONT86_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2352841.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ONT86_002077"
     CDS             378..1739
                     /locus_tag="ONT86_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR2352842.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ONT86_002078"
     CDS             1761..2021
                     /locus_tag="ONT86_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352843.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ONT86_002079"
     CDS             2035..2787
                     /locus_tag="ONT86_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCR2352844.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ONT86_002080"
     CDS             complement(2881..3486)
                     /locus_tag="ONT86_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2352845.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ONT86_002081"
     CDS             complement(3539..4513)
                     /locus_tag="ONT86_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCR2352846.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ONT86_002082"
     CDS             complement(4891..5610)
                     /locus_tag="ONT86_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2352847.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ONT86_002083"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ONT86_002083"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR2352848.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ONT86_002084"
     CDS             7161..8558
                     /locus_tag="ONT86_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2352849.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ONT86_002085"
     CDS             8639..9079
                     /locus_tag="ONT86_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2352850.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ONT86_002086"
     CDS             9227..9553
                     /locus_tag="ONT86_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2352851.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ONT86_002087"
     CDS             9554..10303
                     /locus_tag="ONT86_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352852.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ONT86_002088"
     CDS             complement(10383..10838)
                     /locus_tag="ONT86_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR2352853.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ONT86_002089"
     CDS             complement(11265..11540)
                     /locus_tag="ONT86_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352854.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ONT86_002090"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ONT86_002090"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR2352855.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ONT86_002091"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ONT86_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR2352856.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ONT86_002092"
     CDS             14770..17436
                     /locus_tag="ONT86_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2352857.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ONT86_002093"
     CDS             17800..18552
                     /locus_tag="ONT86_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2352858.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ONT86_002094"
     CDS             complement(18574..19728)
                     /locus_tag="ONT86_002094"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCR2352859.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6330 a         3473 c         4060 g         5958 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat t
//
LOCUS       DAJSBN010000051        19476 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJSBN010000051 DAJSBN010000000
VERSION     DAJSBN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.051"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(22..996)
                     /locus_tag="ONT86_002095"
     CDS             complement(22..996)
                     /locus_tag="ONT86_002095"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR2352860.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1076..1951)
                     /locus_tag="ONT86_002096"
     CDS             complement(1076..1951)
                     /locus_tag="ONT86_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCR2352861.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2081..3988
                     /locus_tag="ONT86_002097"
     CDS             2081..3988
                     /locus_tag="ONT86_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCR2352862.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4529..7807)
                     /locus_tag="ONT86_002098"
     CDS             complement(4529..7807)
                     /locus_tag="ONT86_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCR2352863.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8078..8623)
                     /locus_tag="ONT86_002099"
     CDS             complement(8078..8623)
                     /locus_tag="ONT86_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2352864.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8635..9327)
                     /locus_tag="ONT86_002100"
     CDS             complement(8635..9327)
                     /locus_tag="ONT86_002100"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR2352865.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9346..9630)
                     /locus_tag="ONT86_002101"
     CDS             complement(9346..9630)
                     /locus_tag="ONT86_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352866.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9643..10206)
                     /locus_tag="ONT86_002102"
     CDS             complement(9643..10206)
                     /locus_tag="ONT86_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR2352867.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10434..11096
                     /locus_tag="ONT86_002103"
     CDS             10434..11096
                     /locus_tag="ONT86_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCR2352868.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11220..12416
                     /locus_tag="ONT86_002104"
     CDS             11220..12416
                     /locus_tag="ONT86_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCR2352869.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12517..13893)
                     /locus_tag="ONT86_002105"
     CDS             complement(12517..13893)
                     /locus_tag="ONT86_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2352870.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13930..14796)
                     /locus_tag="ONT86_002106"
     CDS             complement(13930..14796)
                     /locus_tag="ONT86_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCR2352871.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14885..15910)
                     /locus_tag="ONT86_002107"
     CDS             complement(14885..15910)
                     /locus_tag="ONT86_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2352872.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15928..16602)
                     /locus_tag="ONT86_002108"
     CDS             complement(15928..16602)
                     /locus_tag="ONT86_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2352873.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16786..17679)
                     /locus_tag="ONT86_002109"
     CDS             complement(16786..17679)
                     /locus_tag="ONT86_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2352874.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17753..19450)
                     /locus_tag="ONT86_002110"
     CDS             complement(17753..19450)
                     /locus_tag="ONT86_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2352875.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4189 c         3445 g         6158 t
ORIGIN      
        1 ttctttccct cttttttttg tttattcgaa ttctttttct tccagacgga ataacggact
       61 catcggtgta ttttcatgga tgcgtttgac cgcttcaccc attaattctg aacatgtaac
      121 gattgttaag ttttctggat gacgattttc ttctgtgaaa acagaatcag tgatacaaat
      181 atctttgatt ggtgcattgt ctaaaatact tttcgcatga tcggacaata atccatgtga
      241 cgcacaggca tatacttctt ttgcgccgtt ttcttttaat acatttgctg ctcgtgcaag
      301 cgttgctcct gtgttcaaga tatcatctac catgatacat gtctttcctt gaacatcacc
      361 gatcacataa ccaccctcag ttgaagcatc atctgcatga tctacgatgg ctaatgtgct
      421 gttcaaatat tctgaaagac tacgtgcacg ctgaacaccg ctgttttttg gcgaaacaac
      481 tacgatgtca tctcccgtca gacctaattg acggtaataa tgagcaaaca acggcatcgt
      541 aaataaatta tctacaggaa tatcaaagaa tccttgtact tgaaccgtgt gcagatctaa
      601 agtcagtact cgagttgccc ctgcttcttc gatcaagtta gcgatcaatt tcgcagtgat
      661 cggttcatga ggtttcgccg tgcggtcttg cctagcatac ccatagtaag gcaatacgac
      721 attgatcgtt ttcgcacttg ctcgcttcat tgcatcgatc atgatcagta gttccatata
      781 gtagtcattg acaggtgcat tcgttgattg aacgatatac acatgatccc ctcgaatact
      841 ttcttcgata ttgatggaaa tctcgccatc actaaattgc ttcacgacgc ttttacctaa
      901 ttctgttccg aacactttcg cgattttttc agctaatgga cggttgccat tcaaactaaa
      961 aatcttcaac gtgtcatcct gatacttatt actcatgtag tcctccccaa aacttctcta
     1021 ttcttcgata gtttaacata tttttccaag attttctgtt aatttcagca aattcttaag
     1081 gaagtgaaac tgttccgtta ctccaactgg caggaatgac tttatgataa tcgccccagt
     1141 tgtcgtgtac tttgtaataa cgttctcctg tttcagtttc aaaataagcg tacgctgtca
     1201 cccaatgatt gccatatgta ctgccaagta ctttcaaaat accaatcatc acaggtttgc
     1261 cttcacgtat acgttttgta gctcgctgcc atgaacctag aagagttgct cgtgcacgct
     1321 catgattgcc attcttcttt aaaaaactag aaatccctgt actaacctga aaaggaatcg
     1381 taggaagtcc tagtggttgg atctggctac gcaatgcctt cgtcaaaacc aacgaatcag
     1441 ccgtattttt cttgcgaatc tcatttggaa gcatccaccc cttacgatag tcttgataat
     1501 aggcaaggag aactgctgcc gcgtaagtac cacaaagata gcctgagggg gtagcccatt
     1561 tcttatacct caaaaaacgt tcttctggca atcccaccca gcaatttgcg agttcctttc
     1621 ctgacctttt ttcttcgatc aaaacgggaa tatcttcttt tctgttgatc catctagata
     1681 actcgattgg ctcttcattg atcagcttcc attttttaca agaaagaccc tcttgataag
     1741 tatattttgc tggagaatat tctaccggat gtcctgattg atcgataatc acataacttt
     1801 gatcttcaaa aaccagagct aaaaaaagca atttactttt tcttgactgc agctgctgag
     1861 tgctggctaa ttttttaatt tttttatcta ctttgaaata taaaggttta tccactactt
     1921 gattcagttg ggatcgcctc ctttttttca ttataagtga aggattgaaa aaaacaaatc
     1981 agtttgattg ttcgaatttt caatttttta tgttctattt gttaattcct tcattttgta
     2041 agataatggg cacacgaaac aaaaaaggag cgatcgatga atgaacaaag aacgattgaa
     2101 acaagatttc tatgaagcag tcaatgaaga atggcaaaaa acagcaaaaa tccctgcaga
     2161 taaaccagct acaggcggtt tccaagattt agttgacggg atcgaccaac ttttgatggg
     2221 agaaacagac gatctgcttg cccatccaga aaaagtgtct tcagaaaaaa tgaaacattt
     2281 ccttgcatat tatcaactgg caaacgatta tgaccgaaga aatgagttgc atgctcaacc
     2341 gcttcaacct ataatcgaaa aagttgaatc attgcagtct ttcgacgaat tgaataagca
     2401 atttccagaa tggctgttgg atacattacc attgccattt aatctggatg tagatgcaga
     2461 tatgaagaat gcgcaaacga acgccttctt tgcttatccg ccttctttat tccttccaga
     2521 taagacgtat tatgcatcag accatcccaa cggtgcacaa ctaataggcg ttttctttga
     2581 tatggctgtt cagttattag agcttgccgg caaagaaaaa agtcaggcag aagcaatcac
     2641 ggaacaagca gtgcaatttg ataaattgat tgctcctcat gtaaaaagtg cagaagaaaa
     2701 cgcagattac agcaaaatgt ataatccgca atcttttgat acatttatta aatacacaga
     2761 taaactggac ttgaaaaaac ttgtcacggg tttgattggg acaactccag ataaagtgat
     2821 cgtaactgat ccagtgtact tcgatcattt gaatgacatc ctgacagaag ctcattttcc
     2881 gttattcaaa agctggatga tcattaaaac agtacgttct cttagtggtt atctatcaga
     2941 agaattccgc caagtcagcg gaatctattc tcgcgtattg tctggaacag acgaagcaat
     3001 gcctcagaaa aaagcagcgt attaccttgc ctctggacaa ttcgatcaag tagttggcga
     3061 ttactacgga aaaaaatatt ttggcgaagc tgctaaaaaa gacgtagaac aaatggtgaa
     3121 aaaaatgatc gccgtttata aaaaacgtct agaagaaaat gactggcttg gggatgcaac
     3181 aaaagaaaaa gccgtcataa aattaaataa cttaggtatc caagtaggct accccgataa
     3241 gatcccagca ttattcgacc agttcaaaac agtacctgca gatcagggtg gtactttgct
     3301 ttcaaatact cttgttttca gcaaactcac attaaaagat cgtttcagca aatggaacaa
     3361 accagtggat cgttcagaat gggaaatgag tgctgacacg gtcaatgctt attatcatcc
     3421 atttcggaat gtgatcgtct ttccggcagc gatccttcaa gcaccatttt acagtctaga
     3481 acaatcaagc tctgctaatt atggtggaat cggagctgtt atcgctcatg agatttccca
     3541 tgcatttgat aataacggcg ctttatttga cgaatatggc aacttgaaca actggtggac
     3601 agaagaagat cttacacaat ttcaaaacaa agcacaagca atgatcgaag aattcgatgg
     3661 tctgcctttt gctgacggaa aagtaaacgg aaaattaacc gtttcggaaa atattgctga
     3721 tgcaggcggt ctaagctgtg cattggaagc agcaaaaacg gaatcagatc tttctatcga
     3781 ggaattcttt gtcaattggg caacgatttg gcgtacaaaa gcaaaaaaag aataccaaca
     3841 acttttactg caaatcgatg tccatgcccc tgctaaactc cgagcaaacg tgcaaccaaa
     3901 aaaccttgac gaattctatg aaacatttgg tatcacggaa caagacgcta tgtatctagc
     3961 gccagaaaaa agagtgcgaa tctggtaaga ggttgtgcca gaagtggtca gcttcaagaa
     4021 ataagaagga atttgggaaa atcgcttctc aaatttttgt gacatttcgg cttatttttc
     4081 gaagaagtta cttctggaat accatgtatt agaggttgtg aaaaagctgc ttagtttcaa
     4141 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttacta
     4201 ccgagaaact ggcttttgaa caccgtttat cgagaggttg tgaaatcagc gtcctgacct
     4261 gagtattaga gaaaaaaacg gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat
     4321 accgagggtc agctgattga acaccgttta gtggagagat tgtgaaaaag ctgtcctgca
     4381 tcaagtaata agaacagcca atcaaaagca gcaaggagaa cctcatctat aaaaagatag
     4441 aaggaagtga gacatctatt ggtctcattt ccttctattt ttactttcct cagtcaattg
     4501 ttttacatca taaaaggaga aattccactt atttttttcg ctgtttcatg acataaacaa
     4561 ctacaccgat taacaaaatt cctccagcta atgctagcaa cgggttatta aattctcccg
     4621 tttttgggag tccttgagtg ccattttttt cactttcttt tgctgtccta tccggatgag
     4681 aaggttttcc atttggtttt ccatttggtg attcatttcc tggattttct ctttctggtt
     4741 tgtcatttcc cgggggcaca tcggcagttt ctcgcacagt gatcgctttt tgctgggtaa
     4801 cagattctgt atgagcagac catttttccc catcaaatat ttccgaacga taagtgactt
     4861 ctagcgtata ttctcctttt gaaggtgctg aaaaaatagc ggtatgatta gaaaattgag
     4921 cacttgactg tgtttcaacg atttggatct tttctgggaa ataacgcata tcttcaaaga
     4981 caggcgttcc ttgatttggt gtgaaagttg acacaaaaac tgtaaaaggc ttcagttcaa
     5041 tgatatttcc ttctgcattc tccgccacac catttgcttc ttgtataata atcggattat
     5101 tttgttcatt tggttttgtc tgatttggtt cttgtacgat tgcatactct ggatattccg
     5161 aagacggtga ggtttctccg tcaaaaatag cacctacaga taatggaaga ccatccactc
     5221 tttccttttc tgtttgacga ttccccggtt taatctgcat cagtccatga acatggatac
     5281 ttccattttc atagcgcgta acaggctgct gaccttcttt cgaacatgta tttagagact
     5341 cgatccaatc ttttgatatg gtatgtccaa gagtattttc tgattgtttc tctcgttcat
     5401 tccaatagta gttcaacgag tggtagattt gatcttcgtt ttgattcaat gggcggatct
     5461 gctctctcat ctcaagattc tctgttcgga aagataagat accagaagca tggtagtctg
     5521 tgtttcctgc tgaagaggta taaagagcca cattatatga accgttatta aaacttgtac
     5581 tgctattgac gataatatct gttccgctat tggaatcgat tcctttcgca agattttcga
     5641 aagacaaact attgatcaat tcatgagggc ccgttaaaga agaaccgccc attttgaatc
     5701 cattaccgtt accttctccc tcaattcctg gtacccagcc gtttcgataa gcaactgagt
     5761 ttttgatgat tacttttccg atcgaacctg tttcattttt agcaaaaagg tcccaaccgt
     5821 catctgcatt atgatacgcg atacaaccgt caaatacatt tccttctcct attgtcagtt
     5881 tggccgcaaa tccatcggca tcttcaaatc ctggatcggc attttcaaaa gatgtacagt
     5941 tcttgactaa gttatgcgcg ggccatgctt cgaaaggatc atttgagcta ccgctgattt
     6001 ggattcctgt atttccattc cgataagcat gaatctcttc gatcaaatta tgatttccag
     6061 aaatctgtaa tcctttttgc atagctgcac tatttgtaat gtcaaaccct cgtaaacccc
     6121 agtagtgact gaacagtgtc atcccatttc cagttccttt aaaatcaagt acaggacgct
     6181 tgttttctgg atttttctct gcaatcaact gaatcgggtt ttctcttgtc ccgttaacgc
     6241 cttttggaat cgtcaatcct ctttcgaagg aataagttcc aggcgccatg acgattgtct
     6301 gcccagcata tgcgtatctc aatgcttgtg tcagagataa tggattttgt ctgcttccgt
     6361 ttcctgcatc tgacccttct ggtgttacat aaactgtcga atatttaggt ttacggtagt
     6421 ccactgtatg aagaatctct tttgtttcat aactagtcat ctctacgtat tcttctggcg
     6481 gatagttttt atctggcgta aaactgaaag taaattcgtt cgttccttcc atcagttttg
     6541 tctgataatc aaattcccga ttcgcagtga tagccaagtt ttttgcgttc ttaagcacct
     6601 ctcctgttgc tttattttta attgtcaata atccgtcagc atttgctcta aaggacatag
     6661 tatgatcttg gtttcctgaa tgattactag aggtcacagt gtaaatcgga tcaatcaagc
     6721 gagtaggtct ttcttgccca ggaggatctg tcgcaggatc tgagtaagca acttcgattc
     6781 cttctacctt gatcgtcata ttccttgctg cagcaaatcc tgcataaata accgattcat
     6841 ctgaagcgta taatttttcc cagtcataca tgatgtcaga agccatttct tctcctgttt
     6901 caggatcgta ataatatgac tctaaaccag tattcgtttt tttcatctct acgtccagtt
     6961 tagtaatcga gttcgtggcc ggaaacaaat tcccatgacc gtttccgaat atattataag
     7021 aacctgtttc tttttccaag aaccctgctg aagtctctaa aggtgttgtt tccactgaga
     7081 cactcccagg agctggcgca gcttgcggat cttcagatga tattcctgtg attgcccgtg
     7141 tccccaatcc aagtcgcatc gtatatttgc tgccgctcgt gttgaccact tcttgtctat
     7201 ccgagtccca gacatattcg atccgactgc ctagtatggc aaatgaattt gaataaaatg
     7261 cctgcccaaa caaatgattt tttggtactg cgtctcttac cattaaagca aatccttctt
     7321 gggcattcgt ataggtccaa gattctattt cgatcgtcgc ccgcatccta aagttgatgt
     7381 ctttggggat tgcttgataa taataggtca ttccatctga tccgcttgac tggaattttc
     7441 cgccgttatt cgtggaagag agccgcacgc cgtcttctaa tggttcaatg gcatacctac
     7501 tagcacttgt gctttctccg aaaacaaccc ctttccactc ttcatttgct tcttcagatt
     7561 gcttttttgt cttttcattt tctgattctt tcatcatagg atcatttcct tcttccgtat
     7621 ccatatcttc actagttctt ggtacttcct ccattgttga catcccttca atgttcacgt
     7681 gtgcattttc ttcgttcaca gactgttgtt cgttagcagc aacttcaacg acagaaatta
     7741 aaaccggggt actgagcata aggagattca tcattagcgc actgtatttt atacgttttt
     7801 ttaacaagtg tattcctccc gtaacaaatt agaaagcgtt tacttaaagt attacctaat
     7861 ttatttttac tgtcaataaa aaacaaaatt ataataagtg tattttataa aaagaaaaaa
     7921 tataacttaa gttgttgata ataaagagat ttattaacgt aaaaagaata agttttttta
     7981 aaataacgta taaaaaagag cataaaacaa agtcgtttgt tttatgctct tcttttatta
     8041 ttttgtgctt tttattttat caaatcactt tgttttttta taaaatttct gcgcctagtg
     8101 tggactcaaa atgtctcaat gcccattcat gtccaacggg atcgaaactt gccacggcat
     8161 ctttgaaaat atggagtttg tatcctagat tatacgcatc gactgctgta tgcaacacac
     8221 agatatctgt acatacgccg gtcaaataaa tatctgtgat ctggcgttct cgcaaacgga
     8281 tgtctagatc tgttccacta aaagcggaat aatgccgttt gtctatccaa taaacatttt
     8341 cctcttgctc atagtcttgg tataaacttt ttaaagagcc gtacaattcc cgtccacccg
     8401 ttcctatcac attatgcgga ggaaataagc ggttctccgg atgaaaggaa tcttttggat
     8461 catgcgcatc aatggcaaaa acgacaaaat ccttttgatc aataaacttc tttgtatgag
     8521 cagtcagctt tttttcaatt acttgtcctg ctgtcccagt agtcagcttt ccatcctctg
     8581 caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt catcttagtc ctccttatac
     8641 gagatgtttg acaaattcga tcaccatttc tgctgatcgt ttgcctgcgt cttcgataaa
     8701 ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg ctcattgcac gtacgatcaa
     8761 aaatgggata ttgaactgac gagctgtttg tccaacagcg gctccttcca tttcgcaagc
     8821 taatgcttca gggaaattcg ttaagatttc tttgatttta tctggcgagt cgacaaatgt
     8881 atcacctgta acgatcaatc cttcttttgc gttcaaattc gttgcttttg ctgctttaac
     8941 catttcactg cgcaaatatg tgcttgcttc ataatataga ggcattcctg gtaattgtcc
     9001 tggtttgtag ccaaatcctg ttgcatctgc atcaaaataa gctactttgt ctgatataac
     9061 gatgtcacct acttgtaagc cttcaccgat tccgccagct gaacctgtat tgatcaccat
     9121 gttcacgccg tattgttgga tcaacagact tgtggtaata gatgctaaga cttttcctat
     9181 accggaacgt acgacgatca cttcgtggtt acctaatgaa cctgaaataa ataaagcgcc
     9241 tgcgcgttcc catgataatg gttcgcttag gttctctctc aaaattttta cttcttcttc
     9301 cattgcgccg ataataccga ttttcatctt tttaattcct tctttctata taaatgcgat
     9361 tgctaaaaaa accacacata acattaggat aacaataata agagatttca tcaaaaaaga
     9421 gttccatttg gtcgtctttt cccgttcgct gatccttgtt ttcgtgattg gtttattctt
     9481 tttgctttct ttacgataaa agctggcaat ctttttttct tcttgctgat aagctgtttc
     9541 tgcttttcgc tgttttttta atgactcgct tgcttgtgct tgttgtcgct tgcgaagttc
     9601 gcttcgcgta ataagcggtc ctttggccat aagccgatcc ctctactttc ctttggtcaa
     9661 tagttcccag tattgaatcg cataaatcgt ctttgcatca caaatcaact gatctttcat
     9721 cgcttgttgc gcctcttcaa gagttagatg atacaactcc agtacttcgt cttcatcttg
     9781 tgctaaagga ttctcgacct tctctacccc ttgtgcatga taaatatgaa ggacttcatt
     9841 tgcaaaacct ggtgaaagat acatcgaagt aagatggctc agcgacttcg cacgatagcc
     9901 agtttcttct tctaattctc tagctgctgt catttcggga ttttgtcctt ctcctggatc
     9961 gatttttcca gctggaatct ccaaaatcac tttttctaaa ggttttcgaa attgtttgac
    10021 aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg acaccaccag gatgaaatac
    10081 cagttctctc ttcgaagttc ctccatctgg caaacgaact tcgtctacag caacatcaat
    10141 aatttttccg ctatagattt ctttgcgact gattgttttt tcttcaaatt gctcattgtt
    10201 cagcatatct tctcctgctt tctttgtaat cataactgct tttttttcat ttttcaattt
    10261 ttctcaaaaa ctccttatta gtttaccata gataaacctt agattttctc taaatataga
    10321 aaatccttct ttagtccgat actctttcta gtagtccgta tgctaaaata agtatgtaat
    10381 cgaataacga aaagaattca atactcaacg ataaatgaca tggagggttt attatgataa
    10441 aacgaaattg gccgtggctt ctagcattgg tcttgctatt tctctttgtc cgaaacaata
    10501 gtttttcttt tgttctagga cttttattga taggtgtagt tttagttttg tggggactct
    10561 ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc tgcactgact gacgaacttg
    10621 aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat ttcatttttt cgacaaacaa
    10681 tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa tatgcagcag acagccaaac
    10741 ttaaagcaat cgatctccgc catgatccag tcaaagcagc gaaagcttta ttcaaagaat
    10801 tggtgaaaga accaaaccga ctgcatgaag caagtcagtt tctttatacg catctgccga
    10861 atctagttga tttgacaaat aagtataatg agatcaatga ccatgagatc aaaaacaaac
    10921 agacctatga aaaactagaa gaaagcgcgc agatcatcga ccagctgtca gctttaatcg
    10981 cacaagatta tcaaaaattc gttgctgagg atctagatga tatcgacgta gagatatctg
    11041 ttgcgaaaca gagcttaaag cgtgataata aatatgaacc agaacaaaag gattaactct
    11101 tttgttcata aaagaagact cggaacattc agatgatgtc tggctgtttc gagtactttt
    11161 tctaaagtag agacattcat gtaagaatcg agaacagatt caacaaggag gaatttttta
    11221 tggatcataa accgactgaa acaaaagatg ttacaccagt taacgataca cttgaagatt
    11281 tattgaacaa cccgttttca actccaacag atacgctgac cacttcacaa caagcggaga
    11341 ttgatcagct ccaagagcaa cagacagctg ctcgtttaat tgacaaacta ccggcggaac
    11401 gccaagaaca agctaaacag ctagcagcaa aaatcgatgc taatgatgca cagtctgtca
    11461 tcagctacgg atcggctgcc caagcaaaat taagtgaatt ctcacaatcg atgttgaatc
    11521 atgtgcaagc acaagatatt ggtcctgtag gagattcctt aactgaactg atgtatcgtc
    11581 tccaagaagc caatcctgac gaactgcgtg ccggagaagg gaacatcttc cagcgtgtct
    11641 tcgggaaggt caaacaatcg atctatgaag tgacagcaaa atatcaaaag attggtgcac
    11701 aaattgacaa gatttcagtg aaattagata aagaaaaaga cggtctgcta aaagataatc
    11761 tgatgttaga acagctttac cagaaaaaca aagactactt tgatgcactg aatatttata
    11821 tcgctgctgg agaattgaaa atggaagaac ttcaaacaac gatcattcca gaagcgatga
    11881 aaagagcaga agaaactggc gatcagatgg atgttcagat tgccaatgat tacacgcaat
    11941 tcctagatcg tctggataaa cgtacgcatg atcttcgctt agctagacag atcacgatcc
    12001 agcaagcacc acaaatccgt ttgatccaaa acacaaatca ggccttggct gaaaagatcc
    12061 aagcatctat cgctacagcc attccgctgt ggaaaaacca agtcgtgatc gcattgacct
    12121 tgcttcgtca aaaagatgca gtaactgctc aacgccaagt ctctgaaaca acaaacgact
    12181 tattgaagaa aaattcagaa atgctgaaaa tctcagccat cgaaacagcg aaagaaaatg
    12241 aacgtggaat cgtggatatc gagacccttc aaaccactca aaatgatttg atcgaaacaa
    12301 tccaagagac attgcgtatc caaaaagaag gaaaagaaaa acgtcgtcat gcagaagtag
    12361 agcttggaca catggaagaa gatctgaaac aaaaactgtt ggatttaacc caatagatca
    12421 cctttcgctt gttactaaaa caaacgccca acaacagttt attacaactg ttgatgggcg
    12481 tttttcttat ctttcttcta acttctccat tggagtctat ttctcattca tcttgctgtg
    12541 tccatagcca tagccaaaat aaatgatcag tccaataacc attacgatac cgaacacgat
    12601 ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa gtcaagatgg agacgattgg
    12661 caatagcgga acgaatggca ctttgaattc tccagctttt ggtttgccaa acatttttct
    12721 caacttcagt aagcctagac taacaagcat caaataggcg agcgccatga tattcgttag
    12781 ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat acagctgcca caccagcggc
    12841 ataagtcgct tttttcggtg tacgatgctt ttgatcgatt tctgtcataa acttaggaag
    12901 taagccatcc ttgctgacag caaacagtaa acgagcaagt gaatacatca tcgagatcgt
    12961 taccgtcagt agcgttaaaa ttgcccctac agcgatcacc gcgcccactg ttccatgtcc
    13021 tacatacctc attgcaaaag ccactgggtc attgactcct agttcagtat acggaacgat
    13081 tcctgtcaag atcagcgtca cgatgatgta aaggacagta gcaatcagta tcgaaccaat
    13141 gattcctctt ggcacatctt tttgcgggtt tttgacttcc tcggctgcca ttgcaacggc
    13201 atcaaatccc aagaaagcaa aaaacacaag agctgcacca tctagcacac cggaaaaacc
    13261 aaaaggcatg aatggctgcc agttatcagg tttaacgaaa aatattccta caatgataaa
    13321 caacgcgatg ataccaaatt tgacatatac catggcatta ttcaaacgta atgctttctt
    13381 ggcttcaagc gacacgatgt aagtgacgaa tatcactacg agcaccgcaa tcaagtcaat
    13441 gtatgtacca ttttccggat tgtacccgcc agttaatgct tgtggaagtt cggcccctaa
    13501 acttttcaaa aaaccttgca tatagccaga ccatccggaa gcaacagagg aaaccgctag
    13561 taaaaattcc ccgatcaaca accaccctgt cagccaggca gcaaattcac caaatacaac
    13621 atacagatag gcatatggcc caccaatcac tggcactcgc gaagcaaatt ctgcgaaact
    13681 tagtccagac aaaatgacaa cgattgcagc taagacaaat gaaagtgaca gtgctggacc
    13741 agcattttga ttggccgcaa cccctgtcac cacaaatatt cccgtaccga tgatcgcacc
    13801 aattccaagc atgatcaaat cagacgtttt tagttctttt ttcatctcac tcgtatgatt
    13861 gagattgatc tctttcttac gaaacaaatc cattgaagtt cctccccatt ttaggttgga
    13921 aaacagcgat taaactgttt tcatataccc taaaaggata ccacctaaaa tgactaaaag
    13981 gcaaccaaca ataacataaa tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat
    14041 cccacctaaa gtagagataa tgatccccat ttgtgaaagt gagaagccaa cagctaaacc
    14101 aagtgatttg actgttagaa gcatgcagat gtttcctaag ccccataata atccacagat
    14161 catgtttttc cacacaaact gatcgacttt aactttacgg aaagcaaaga agcttgcgcc
    14221 taagatcatt ccgatacttt gaggtaagat aattgacatc gcatccaaat tcgcccagtt
    14281 gacgatgatg gtatatacac cataaccaat cgtagagaag atcaatgctc taaaaccctt
    14341 accaaaatca agcattttat tatctgtatc tatttttcct tcgtcatctt gtcgggcagt
    14401 caaatacgca ccgcttacaa gaagagctaa agcaataata ccgtatacat aatctttcgt
    14461 ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc
    14521 agtagacaat ggtagcccta cagaaacacc catgtacttc attccatgga actgtccatt
    14581 ttgtccaaca ctccaagcta agccggataa aaatccgatc aagaaaatcc aaggggtgat
    14641 tacaggacta acaacgaaaa agacaactaa agaaaacaga aatgcaccga tcgtcattcc
    14701 tagtgtttgt tgattggcat cgccaccaat tttaccactt actaaaccaa tactacccca
    14761 tgccaacata gggactaatg ctattaaaat ttccataacc aatctccttt tatttcacat
    14821 gataagaaac ttatcagcga aaacgttaac aacacaagca ctttttttcg tttctaacga
    14881 ttttctaacg tttttcatta taaaaattta agattgggaa aaataaagtc attgttgtcc
    14941 cttttccttc agttgattca gccgtcaaat ggatacctaa tttttcggcc agattttttg
    15001 ctaaataaag tcctaatcct gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa
    15061 atcccttatc aaagatccgt ttcaagtctt ccgctgggat accgatccct gaatctttaa
    15121 ctgaaaggct gactccttgc gcttcctttt ctatgctcac aatgatgttt ccgtgatccg
    15181 gcgtatattt gatggcatta ctaagcaatt ggttgaagat aaaagcgatc catttagcat
    15241 cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg aatatttttt tggataaaat
    15301 aatttgcctg actgcgtatc acaggctgaa tgattccttt tagtccatat tcttggatga
    15361 ggtagtcccg cgagaaactg tcgagtcttg cataatacaa aacttgttct acgtattcat
    15421 cgatttttga taattcattt tctagtaatg tatacttttg atcgtcaata tcgaactcga
    15481 tcgaacgaac tagcaattta gaagcggaaa gtggcacttt gatttcatgg acccatgagt
    15541 caatataatc tttttggtct tgttgattac tgattgctga ttgcattact tcttggtgtt
    15601 cacggatcag ctgattgata tattcttcta ccagtttttc ttcttctgtc ctcccaccac
    15661 tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc taattttccc caccatcttc
    15721 tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa gacgccctca atcaaagcta
    15781 aataagcaat tgtgctcaaa tcaactttga tgtttggcgc cagccacatc acaaagatcg
    15841 tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca ctgatctttc aaatatttaa
    15901 aaaaattcat cttaaccgct catcctttca gggaacgata tatccctgtc ctacttttgt
    15961 ttcgatatat ccttggatac ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac
    16021 tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac agtgctcgca gcaatttttc
    16081 ccgacttaag atctttccat tgtgcttcat caaaataaat aatagacgat attcattttt
    16141 gctcaaatca atgatctctc cgttgatttc catacttccg ttgtcaacat tcaacgtaat
    16201 gccattgtgg ctcatcattt cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa
    16261 tgcattgatt tttgcaatca gtacatcgat ttggaaatgt tttgtcacaa aatcatctgc
    16321 ccccatgttc atagccatga tcatatccat attcgtattc ctgctggaga taaaaatgat
    16381 cggtactttc gatacttcac gtattttttg acaccaataa tagccatcca aaacgggaag
    16441 attaatatct agtaatacaa gctgtggttc ttctctttca aagtctgaga acacttgatt
    16501 gaaatcggtc gttccaaagg tttcaaattg ccatttctca agttcctcca tgatcaattg
    16561 tcgaatgatc tcttcgtctt cgactaccat gatttttgcc ataaaaaatc cctccatctt
    16621 tcttcttgct actacaatat caaagaagcc cttgtgaaac aagttggctt acctggaaaa
    16681 tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc gtttgtctct
    16741 tccaagcagc ttaaatttat tatgttattc ttaataattg gtctattatc tgttattttt
    16801 ctttcgttgt ttttgtcttt taaattttcg ataacaccaa taaccgaaaa gcgttagcac
    16861 tagcaatata cctagaagga gtataagtaa tcgataaaga tggtattttt ttgtctgttc
    16921 tatctttgaa gagacttctt ctgtaaaggg aactcgtctt cctgtcacta gtaatcggtg
    16981 agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact agatcctgct tttctctaat
    17041 tgctaatgta tttgtttttg ttggaaggac aacaattttc tcaacgactt cataagcgag
    17101 catccgacca tacacttcaa gatagaatcg gtcatcttgc tccatttctt caagatccgt
    17161 aaagagtttt ttctcagcaa taccgctatg ggcggtaatc acggaatgtg tactctctcc
    17221 tccagttgga aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca aaagatcatt
    17281 cgtttcatca aataaaggca agctaacatt gattttcgga atatagatgg ctccgatcat
    17341 atgttgttcg taatattctt ttcctggatt ttggacattt ctaaccgctt gctcaaaagg
    17401 atctttgact tgtcccattc ctgggattac tgatgtatga gcaagcgtct gatttttctt
    17461 ttcatgcact gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg
    17521 ataattctcg atccttcttt gatccaaata attgctgaga gcatctgcta aaaatggata
    17581 tgtaaaaact gcagctccgg caagaaacat cactgccata aataattgta ataaaagact
    17641 gatttgggtt tgaggtctat tacctatttt ctttttcatt ttcttcacct tcttatttaa
    17701 aaaaactgca gcaaccttta taaaaccgat tgctgcagct tatttttgtt gcttatactt
    17761 cagacttcat tttagattta cggtaccagc tgtacgctcc aatcatcaaa cttattccga
    17821 ttagtaagaa tgccaagagc ccgttcccac cagtagcagg aagtgacccg cgtttcatat
    17881 tcggtactct ttctggccct ggattttcta ctcctacgat ctgaatttct tcattatagc
    17941 ttccataaga gatgataaat tcagtaaagg ctgcctcacc tgttggaagt acataacctt
    18001 ctggcgccat ggtctctttc atttgatagg ttccataatc taacccttga acctcaaagt
    18061 atccatttcc atctgcgaca aattctgttg cttgtccgct tgttgcccac tcaattctat
    18121 ctgctcctga ttcataaaca gctaaaggcg actccccttt ataaaatatt gcgtattcag
    18181 aacggtcttc attccacaat tcgaatcttg catctttaag caattcactg cttgatgcat
    18241 catatttttc aaactttctt ccacctgttg tgactttctc tggcggctct actccttctt
    18301 cataatcaaa ttcatcattt cgtccgattt caataaccgc tgtgttgtta tggaaatcat
    18361 ccgcttctag ttctgtggaa accttcaaat tatacgtgat cgttaaatct cctccacgtg
    18421 catctgctaa tctacctaaa gtatctactt tggctactcc attttcttct gtcaaaatcg
    18481 tcacgacaaa cccgttgctt actacttcta ctatataatc ttgtcctttt gttaaaatcg
    18541 tctcgccggc tcgcacttct atttcctgat cataataagt tagaccaact gtaggcgtat
    18601 ctgtgatttt gtaatttttg ataacagcgc ttccgttttc aaggacagaa tcgatgaaat
    18661 aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc tctttctaga tcaatataag
    18721 aaatcgtttc ttgttctcca gcttcattaa taatcgttac ttgtctaagg tcgctttcct
    18781 ctacattcat ctgtttatct gcttctctga tttcgttttt cggatacaaa tgcacatctg
    18841 tattgtatgt gtctttccat gtagaaccat cccacacgcc atccaccata tcaggcatca
    18901 tcactggcat cataacaacc attggagacg cgattctgga aatatgagct ggcgaataag
    18961 tttctaagaa aagatagact ttatctctag aagaaggtgc ttcttccgtc acttggacgg
    19021 tagagaatac cgcttctcca gcttcattcg ttgtttgttt gtcaatactt gtcagtcgat
    19081 attttaaaaa ccaactttct gtgtgccgtt tttgaatcca ttcaatcgct tcggcagaat
    19141 taagaccgtc ttctcgttcc ggtgttaaag gattatctcg aatcaaatcg taatatactt
    19201 ccgttacgtc aaacaaatca aaatcgactc ctggaatatt ttcttcacca aaatccgtag
    19261 aaaccaatcc gctattcggt cgttcttccg gaacagatga gaaacctttt ttgtgtaatg
    19321 taatcgtcac ttcacttggt ttttgtgcga cttcttcggc tttgactagt tttccgttcc
    19381 cgaaaatccc tattcccgca gtaacgagta acagtacact cattcccgtg acaatcattt
    19441 ttttattcat ttttttctcc ccgttctttt tttatt
//
LOCUS       DAJSBN010000052        18741 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJSBN010000052 DAJSBN010000000
VERSION     DAJSBN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.052"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            257..532
                     /locus_tag="ONT86_002111"
     CDS             257..532
                     /locus_tag="ONT86_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352876.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2617
                     /locus_tag="ONT86_002112"
     CDS             545..2617
                     /locus_tag="ONT86_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCR2352877.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2782..4668
                     /locus_tag="ONT86_002113"
     CDS             2782..4668
                     /locus_tag="ONT86_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2352878.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4680..5627
                     /locus_tag="ONT86_002114"
     CDS             4680..5627
                     /locus_tag="ONT86_002114"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR2352879.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5647..6174
                     /locus_tag="ONT86_002115"
     CDS             5647..6174
                     /locus_tag="ONT86_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR2352880.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6237..6890)
                     /locus_tag="ONT86_002116"
     CDS             complement(6237..6890)
                     /locus_tag="ONT86_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR2352881.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7022..7864
                     /locus_tag="ONT86_002117"
     CDS             7022..7864
                     /locus_tag="ONT86_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2352882.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8022..8918
                     /locus_tag="ONT86_002118"
     CDS             8022..8918
                     /locus_tag="ONT86_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR2352883.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8921..9634
                     /locus_tag="ONT86_002119"
     CDS             8921..9634
                     /locus_tag="ONT86_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCR2352884.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9654..10172
                     /gene="msrA"
                     /locus_tag="ONT86_002120"
     CDS             9654..10172
                     /gene="msrA"
                     /locus_tag="ONT86_002120"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR2352885.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10169..10396
                     /locus_tag="ONT86_002121"
     CDS             10169..10396
                     /locus_tag="ONT86_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCR2352886.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10548..11099
                     /gene="lepB"
                     /locus_tag="ONT86_002122"
     CDS             10548..11099
                     /gene="lepB"
                     /locus_tag="ONT86_002122"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR2352887.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11182..11682)
                     /locus_tag="ONT86_002123"
     CDS             complement(11182..11682)
                     /locus_tag="ONT86_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR2352888.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11961..12359)
                     /locus_tag="ONT86_002124"
     CDS             complement(11961..12359)
                     /locus_tag="ONT86_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2352889.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12619..13479
                     /gene="ylqF"
                     /locus_tag="ONT86_002125"
     CDS             12619..13479
                     /gene="ylqF"
                     /locus_tag="ONT86_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCR2352890.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13472..14239
                     /locus_tag="ONT86_002126"
     CDS             13472..14239
                     /locus_tag="ONT86_002126"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR2352891.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14295..15152
                     /gene="dprA"
                     /locus_tag="ONT86_002127"
     CDS             14295..15152
                     /gene="dprA"
                     /locus_tag="ONT86_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR2352892.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15274..17352
                     /gene="topA"
                     /locus_tag="ONT86_002128"
     CDS             15274..17352
                     /gene="topA"
                     /locus_tag="ONT86_002128"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR2352893.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17321..18697
                     /gene="trmFO"
                     /locus_tag="ONT86_002129"
     CDS             17321..18697
                     /gene="trmFO"
                     /locus_tag="ONT86_002129"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCR2352894.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5093 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag
     2701 aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa
     2761 tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact
     2821 tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc
     2881 gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc
     2941 gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat
     3001 ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga
     3061 aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa
     3121 aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa
     3181 gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt
     3241 gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg
     3301 caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat
     3361 tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg
     3421 gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt
     3481 ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt
     3541 gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct
     3601 tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc
     3661 gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta
     3721 gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat
     3781 gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt
     3841 actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc
     3901 ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa
     3961 aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa
     4021 gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt
     4081 ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta
     4141 tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat
     4201 gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct
     4261 gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta
     4321 ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag
     4381 caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct
     4441 gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag
     4501 attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat
     4561 aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa
     4621 gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga
     4681 tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag
     4741 acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa
     4801 aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc
     4861 tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt
     4921 gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga
     4981 ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga
     5041 gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc
     5101 ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg
     5161 atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg
     5221 tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt
     5281 ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg
     5341 tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac
     5401 atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata
     5461 tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt
     5521 tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag
     5581 aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat
     5641 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
     5701 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
     5761 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
     5821 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
     5881 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
     5941 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
     6001 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
     6061 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
     6121 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
     6181 tatggataga ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa
     6241 gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa
     6301 acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa
     6361 agcccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca
     6421 gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact
     6481 ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag
     6541 attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta
     6601 taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt
     6661 gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc
     6721 gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact
     6781 aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta
     6841 aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct
     6901 cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat
     6961 tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat
     7021 aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga
     7081 tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga
     7141 tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc
     7201 caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga
     7261 cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc
     7321 aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga
     7381 ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt
     7441 agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat
     7501 atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc
     7561 tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac
     7621 taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa
     7681 agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca
     7741 agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac
     7801 gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt attatgtaga
     7861 ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc tctttttctt
     7921 tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt tgataaaatt
     7981 tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac
     8041 atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg
     8101 cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa
     8161 ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag
     8221 atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata
     8281 atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct
     8341 taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca
     8401 cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa
     8461 cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg
     8521 aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat
     8581 tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag
     8641 gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc
     8701 tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta
     8761 gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc
     8821 atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga
     8881 cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt
     8941 atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gccttcctta tccttcttgg
     9001 ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga
     9061 actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc
     9121 aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag
     9181 tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta ctttcgaagt
     9241 actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg aaaacggaaa
     9301 tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa tcaaagatgt
     9361 gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca atacagatga
     9421 tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt atattaaagc
     9481 agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc caaaagatac
     9541 ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta ctgaaagcaa
     9601 gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc ataatggaac
     9661 gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac acgcttcctg
     9721 gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg acttatgaac
     9781 aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat gatccaacat
     9841 tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca actgacgctt
     9901 tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat ttgacagaag
     9961 aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac
    10021 agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa gaagaccatc
    10081 aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct
    10141 ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt atttaatgac
    10201 actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag ccggtaaaga
    10261 catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc tggaactgaa
    10321 tgtcgactat ttgccgtcca tggatatctt tgacgaagca tgggaaaaat atttagaaaa
    10381 taatcatgac atataagaga tagttttgta ctttatgatc aaaaaagttt tttgtccaat
    10441 agcctaattg aaagaattat ttttatttca gctatctagt cgcatttttt tgatattttt
    10501 cttataataa agaaagaatg aaaaaaagag ggatggattg atagagattg acaaaaaaac
    10561 aacgctatat taatcgcttc tggcttgtat tcaaatactt acttgtttct gtattccttg
    10621 cgttcatgtt gcggggattt ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa
    10681 acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt ttcggagatc agacgatttg
    10741 atattgttgt ttttcaatta gcagatggca cgatttatat caaacgagta atcggtcttc
    10801 caggagaaaa tgtcagctac caaaatgatc aattgaagat caatgggaaa gtagttaagg
    10861 aaccttatct aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat acgacggact
    10921 ttacactaca agagctaacg ggacaatcca agcttccgga agataattat tttgtacttg
    10981 gagataatcg aagagtttca aaagatagtc gttcgtttgg cacgatcaat aaaactgata
    11041 ttctaggtaa agcaagattt gtctattatc cacttgatga aataaaatgg atccagtgag
    11101 aaacaataaa gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc caaaatcagt
    11161 ttactacctt agtacgtaaa atcaaacagc tcgtttaaac acttttctag cctctaaagt
    11221 tttgataaat gaataaacaa ccattatttg aatagggatt tggatcatat tcctgatgaa
    11281 tctacctaga aaaattactt tgaaatctgc accaaacaaa acagacatca gccaagaatt
    11341 gataaaaagg ctgactggga tcataatcaa taattgggca gcgatgacgg aaggcaggtt
    11401 tatcttttta tgatataaaa ataagccatt aataaatcca ccaaggaaag ccgtgataag
    11461 aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat
    11521 ttcaagcaaa ccagcccaga tcggtccata tagataaccc ataagcgcca tacttaaaaa
    11581 tccgaatgtg atttgaaaat aagcaggacc aatcgctatc ttgtataaga tgacatcaag
    11641 ggctaataaa agagcagagg tcgacaacaa tcttgcattc atttcgggca atctaaaaac
    11701 gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg tcagttaata ataaaaggat
    11761 accataaaat caccaatgtg tcatgacaca ttggtgattt acatacactg gcatctaaca
    11821 attaaaaaaa gggtgagaag actgctttta taaactgtaa aaaagagatt gtacgaataa
    11881 aaaaaataga ggcggagaat acttatcgta tcatccgcct ttgttttttt gttataaaaa
    11941 acgtcagatt agttatctgc ttacatcttc atccatacgg gttctaatgt atttccgtct
    12001 agatcttgga cttctaatga aaacatttct tcttcaggca ttcccatatc tacttcgtgg
    12061 aaagtaccac cattttcttt tgcaatttga gcaaattttt ttacttcatc cgcgttttca
    12121 acactaaacg aaatcaaagc accacttgtt tttttattat cagctatttc tttatctttt
    12181 ataaattttt tataaaaatc atgagagaga agcattatcc aaaaagtgtc atcccatacc
    12241 attgaaccag cttcttcagt agaaaattct tcattttttt taaaacccag cttttcataa
    12301 aaagcaacgg aacgattgac atcagcaaca ggaaaattaa caaaaaccat actattcatt
    12361 tgtttcgtct cctttctgtt ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa
    12421 aatttagatt catatataga aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg
    12481 tgatagaatg aggactggtt cactttcact taactaatag atggaatttc tttataataa
    12541 aaaagtgaat ttatattgaa aaagaaaaaa acaagtaaag ccggggaata ttccggtatg
    12601 aaaggaatgg agagaacaat gaccatccaa tggtttccgg gacatatggc aaaagcccga
    12661 cgagaagttt ctgaaaaaat gaaatatgtg gacatcgtat ttgaattggt agacgcacga
    12721 ttacctttat cttctcgtaa tccaatgcta gatcagatca ttcagcaaaa acctcgcctt
    12781 gttttattga ataaagcgga tctggcagat ccacaacaga caaagctgtg gcaagaatat
    12841 tttcgtaaac aaggccatct tgcattagct atcacggcac aagaaagtaa aggaattaaa
    12901 ggtctagtac ctgcggctaa agaagcattg aaagaaaaac gggcaagaga tgctgctcgg
    12961 gggatcaagc ctcgcgctat tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct
    13021 acgttgatga atcgactagt aggcaagaaa attgcacaga caggaaataa gcctggcgta
    13081 acaaaaggtc agcagtggct tcgtttaggt aatgagttgg aattgttaga cacaccaggg
    13141 attttatggc ccaaatttga agatgaagaa atcggcaaaa aactagcgct gacaggcgcg
    13201 atcaaagacc agcttgtcca tttagatgat ttagcgattt acggattaga cttttttgcc
    13261 cgatattatt caggaagaat cagcgatcgt tatggtttat caatcgagca agaagaaaaa
    13321 ttggcaccgg aattactgat ggatattaca gcaaaacgtg ggtataaaga agactatgat
    13381 cgtggcagcg aattagtcgt gcatgatatt cgtcaaggaa aacttggacg atacacactg
    13441 gatcgttgcg aagaaatgga gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga
    13501 acgattgaaa agcatacaga gaatagatga tcctacacta gaatcattaa gaaatgatcc
    13561 acgcaaaggc gtgcagcaag cactggcttc ttttgaacga cgattgaaaa aggaagcatt
    13621 aattaaggaa aaatatgaag aaatgttttg ctttgaaaag caagggtacg cacaaggtca
    13681 ccaatggatt gccggcattg atgaagtagg aagaggacca ttagctggac ctgtcgtggc
    13741 agctgcagtt atcttgccga aaaatgagat catattaggg ctgaatgact ccaaacaatt
    13801 atcagagaaa aagcgagaac ttctttatca acaagttcaa gaaaaagccc tggcaatcgg
    13861 gatcgggatc gttgatcagc agacaatcga tgatgtgaat atttatgaag caagtaaatt
    13921 agcgatgatc caagcagtag aaaaattgtc cattgtacca agctatttgt tgatcgatgc
    13981 gatgaccttg gatttgccta ttcagcagga aaaaataatc aaaggggatg cacgatctgt
    14041 gtcgattgct gctgcaagta tcattgctaa agtatatcga gaccaattga tgaaagaata
    14101 tgacgtgctt tatcctggat atggtttctc gcataatgca ggttatggaa caaaggaaca
    14161 cttagaagga cttgaacgac aaggaattac gccaatccat cgtaaaagtt ttgcacctgt
    14221 aaaaaaatat ttacaataaa aaacagccgc ttttacgtat cttattagaa agagagacga
    14281 aaggcggttt tatgatgtat caaatagaag aaaatttatt gaaacttgct tatacaaaag
    14341 gaatcagtag tcaaggaaaa tgggcacttg ctaactggat gatgcagtat ccttataaag
    14401 aaatcggatt tgaagacatc attaagatag gtaaaataac caaacatcgg gagctgtttc
    14461 gcaaaagttg gcaggaaatt cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta
    14521 tcacttgctt tgatcccaaa tatccccctc agctgcttca cttgacctat ccgccaattg
    14581 tactgtttta taatggtgat ctatctttac tttcttgtaa catgttaagc gttgttggtg
    14641 gaagacacac ttctgcttta gccaaaaaaa cagtttatta tttactaaaa ccagtgatag
    14701 aagctggata tgtgatcgtc agtggctgcg cgaaagggat agatacatac ggtcaccaag
    14761 cagcaatctc tcatggtgga cggacaatcg cagtcattgg aacagggata gagcaagtct
    14821 atcctgctga aaatcgtgac ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg
    14881 aatatcctcc tcaatccggc ccgaaaaagc attgttttcc aatgagaaat cggattatcg
    14941 cagctttgtc taaaggaaca tgtgtctttg aagccaagaa aaaaagcggc tctctgataa
    15001 cagcacagca agcattggat cttggaagaa cagttttttc tgttccagga aatattttaa
    15061 cagaacattc taccggatgt catcatttga tccaagacgg tgcagtttgc acggtttcgg
    15121 gtgaagatat cttggcagaa tttaaagaat aacaccatac ttttcatttg cttttcttga
    15181 caaacgtttt ctgaatggat atgattagct acgatttgta tgaatcctct tttaatagta
    15241 ggaacacgac aatggaaagg agtccattca gtaatggcgt ataaatattt agtgattgta
    15301 gaatcacctg caaaagccaa gacaattgag aaatatcttg gacgaaacta taaagtaatg
    15361 gccagtgttg gccatataag agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac
    15421 aattatgaac cccattatat ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa
    15481 gccgcagcaa aaaaagctca aaaagtttac ttggcaagtg acccggatag agaaggagaa
    15541 gcgattgctt ggcatttagc gtacctactt gggttggatc tgaaagaaaa aaatcgggtg
    15601 gtcttcaatg aaatcacaaa agacgcagtc aaagcagctt ttaaggaacc aagaacgatc
    15661 gatgtagatt tagtagatgc acagcaagct cgtcgtacct tagacagaat cgttggttat
    15721 tcgatcagtc ctattctctg gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa
    15781 tctgtcgctt taaaaattat tattgaccgt gaaaaagaga tccgagaatt tgttccagaa
    15841 gaatattgga gcatcgacgg taattttaaa aaagctcgca agaaattcaa agcaaatttc
    15901 tggggaatcg acggtaagaa aaagaaatta ccagatgcac aaagtgtaaa agaagtcact
    15961 gctagaattg atggaacaga cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga
    16021 aaccctgcga atcctttcac tacaagtagc ctgcaacaag aagctgcccg taagttaaac
    16081 tttcgaacaa gaaaaaccat gatggtagca cagcaattat atgaaggaat cactttgggt
    16141 aaacaaggaa cagtcggctt gattacatac atgcgtactg attctacccg gattgccgat
    16201 acagcaaaag cagaggccgc agcatttatt gaagatacgt atggaaaaga attttctaga
    16261 catgatcacc gaaagatcaa aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt
    16321 ccaacgagtg tgcttcgtac accagatgaa ctcaaacaat atttagataa agatcaattg
    16381 aaactttata cattgatctg gtcacgtttt gtggctagtc agatgacacc agctatttta
    16441 gatacaatga aagtgacatt agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa
    16501 atcaaattca aaggattcat gcaagtgtat gtggaaggta gagatgacgg caaagaagag
    16561 aaagaaaata ttcttccaga acttgaagaa ggcgatgtag tccaatctgt tgatattgaa
    16621 ccaaaacaac attttactca accaccagca cgttttagtg aggctacgtt gattcgtgca
    16681 ctagaagaaa acggggtagg gcgaccttcc acttatgcac caacacttga tacgatccaa
    16741 agacgctact atgtaaaatt gacacaaaaa agatttgagc cgactgaact aggagaaatt
    16801 gtcaattcat taatctgtga attcttccct caaatcgtcg atatccattt cacagcagaa
    16861 atggaaggag atttggataa aattgaagaa ggcacggaag cgtgggtcaa agtggttgat
    16921 cgtttctata aaccgtttga aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt
    16981 caaatcaaag atgaaccagc aggattcgat tgtgatgtct gtggacatcc gatggtgata
    17041 aaattaggga aatatgggaa gttttatgct tgcagtaatt ttcctgattg tcggaatacg
    17101 aaaccaatcg taaaagaaat cggtgtgact tgcccagtat gtcatgaagg acaagtcatt
    17161 gaacgaaaat ctaagaaaaa cagactattc tatggttgtt cccgtttccc agaatgtgaa
    17221 ttcacttctt gggataaacc agtgggcaga aattgtccaa aatgtgacgg atatctggtc
    17281 gagaagaaag tcaaaggcgg taaacaagta gtctgtgtaa atggagatta cgaagaagac
    17341 atccaaaaat aatagaaaag aggttctttc attgaaaatg gtaaatgttg ttggagcagg
    17401 gcttgccgga agtgaagctg cttggcagat cgcacaagca ggagtccctg tccgtttata
    17461 tgaaatgcgt ccagtcaaat cgactgaggc gcatcacaca agtaattttg ctgaactagt
    17521 atgttccaat tcattaagag gaaacagttt ggctaatgca gtagggttat tgaaagaaga
    17581 aatgcgccga ttgaattcgg tagtaatcac ttcggcagat gaaacagcag tgccagctgg
    17641 tggtgcctta gctgttgacc gcgattcttt ttctgaaaca attacccgaa aagtcaaaga
    17701 gcatccatta gtaacagtga taaacgaaga aattactgaa ataccggaag gaatcaccgt
    17761 agtagcaaca ggacctttga cttctcatcc attagcagaa agcatcaaag cattcaatgg
    17821 ttcagatggt ttttatttct atgatgcagc tgcaccaatc gtagacaaag ctacgatcga
    17881 tatggataaa gtatatttga agtcccgcta tgacaaagga gaagcagctt acttgaactg
    17941 tccgatgaca gaagaggaat tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc
    18001 agaacttaaa tcttttgaga aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat
    18061 ggcacagcga ggattcaaaa cgatgctttt cggacctatg aaaccagttg gattggaaga
    18121 tcctaaaact ggcaaacgtc cttatgctgt tatccagctg cgtcaagaca atgctgcagc
    18181 ttctttatac aacatcgtgg gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt
    18241 tttccggatg attcctgggt tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg
    18301 aaacagcttt atgaattcgc cagaattgct gaaaccgact taccaatcaa cgaaacgcga
    18361 agatctgttc tttgcaggtc agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag
    18421 cggactactt gctggaatca acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt
    18481 tccacaagaa acagctattg gaagtatggc ttactacatt actcatgcgg aaggaaaaca
    18541 tttccaaccg atgaatgcca actatggtct gtttcctgaa ctgccagagc gcattcgaga
    18601 caaaaaagca agatatgaag caattgcaca acgagcttta aaagctaatg aggcaataaa
    18661 aacagaatta gaaaaaactg caatcccaaa tgagtaagga aactccatga agagggtgag
    18721 acggatagtc tcgctctttt t
//
LOCUS       DAJSBN010000053        18057 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJSBN010000053 DAJSBN010000000
VERSION     DAJSBN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.053"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            99..293
                     /locus_tag="ONT86_002130"
     CDS             99..293
                     /locus_tag="ONT86_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352895.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="ONT86_002131"
     CDS             562..861
                     /locus_tag="ONT86_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352896.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="ONT86_002132"
     CDS             complement(958..1392)
                     /locus_tag="ONT86_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2352897.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="ONT86_002133"
     CDS             complement(1624..2334)
                     /locus_tag="ONT86_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR2352898.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="ONT86_002134"
     CDS             complement(2324..3184)
                     /locus_tag="ONT86_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCR2352899.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="ONT86_002135"
     CDS             complement(3189..3827)
                     /locus_tag="ONT86_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR2352900.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="ONT86_002136"
     CDS             complement(3842..4141)
                     /locus_tag="ONT86_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2352901.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="ONT86_002137"
     CDS             complement(4184..5668)
                     /locus_tag="ONT86_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR2352902.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="ONT86_002138"
     CDS             complement(5688..6149)
                     /locus_tag="ONT86_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2352903.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="ONT86_002139"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="ONT86_002139"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR2352904.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="ONT86_002140"
     CDS             7452..8222
                     /locus_tag="ONT86_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2352905.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="ONT86_002141"
     CDS             complement(8495..9322)
                     /locus_tag="ONT86_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2352906.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="ONT86_002142"
     CDS             9890..11125
                     /locus_tag="ONT86_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR2352907.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="ONT86_002143"
     CDS             complement(12124..12384)
                     /locus_tag="ONT86_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR2352908.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="ONT86_002144"
     CDS             complement(12411..13712)
                     /locus_tag="ONT86_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2352909.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14004..14462)
                     /locus_tag="ONT86_002145"
     CDS             complement(14004..14462)
                     /locus_tag="ONT86_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2352910.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14475..15806)
                     /locus_tag="ONT86_002146"
     CDS             complement(14475..15806)
                     /locus_tag="ONT86_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HCR2352911.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15817..17259)
                     /locus_tag="ONT86_002147"
     CDS             complement(15817..17259)
                     /locus_tag="ONT86_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2352912.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17246..18010)
                     /locus_tag="ONT86_002148"
     CDS             complement(17246..18010)
                     /locus_tag="ONT86_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HCR2352913.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
BASE COUNT         5746 a         3732 c         2789 g         5790 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt
    13861 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
    13921 ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc
    13981 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
    14041 tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta
    14101 tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt
    14161 acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct
    14221 aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg
    14281 gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc
    14341 gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt
    14401 aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc
    14461 actttttatc acctctactt taatgctggc caatattttt tgtttacttt gatcatttca
    14521 tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt
    14581 aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact
    14641 tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc
    14701 acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga
    14761 atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa
    14821 atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc
    14881 acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg
    14941 accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag
    15001 taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc
    15061 atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct
    15121 tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa
    15181 atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga
    15241 tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta
    15301 atcattttta cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt
    15361 gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat
    15421 aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct
    15481 actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca
    15541 cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca
    15601 cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata
    15661 tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt
    15721 tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata
    15781 gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa
    15841 ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg
    15901 tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc
    15961 cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc
    16021 tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa
    16081 ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt
    16141 ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa
    16201 aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc
    16261 aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc
    16321 tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact
    16381 aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat
    16441 tcctttgacc atctctttat tttctttata agctgcttta tagatagcta gacatatagc
    16501 tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc
    16561 tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct
    16621 tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa
    16681 acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc
    16741 aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat
    16801 tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac
    16861 tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat
    16921 aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa
    16981 ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa
    17041 accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc
    17101 tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc
    17161 taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt
    17221 aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag
    17281 gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac
    17341 gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca
    17401 tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca
    17461 cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct
    17521 caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat
    17581 catatttttt tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat
    17641 gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat
    17701 tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg
    17761 atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg
    17821 tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat
    17881 gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag
    17941 catccataat cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca
    18001 cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatc
//
LOCUS       DAJSBN010000054        18037 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJSBN010000054 DAJSBN010000000
VERSION     DAJSBN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.054"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(27..725)
                     /locus_tag="ONT86_002149"
     CDS             complement(27..725)
                     /locus_tag="ONT86_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCR2352914.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="ONT86_002150"
     CDS             complement(718..2070)
                     /locus_tag="ONT86_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCR2352915.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="ONT86_002151"
     CDS             complement(2293..3693)
                     /locus_tag="ONT86_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCR2352916.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="ONT86_002152"
     CDS             3854..4591
                     /locus_tag="ONT86_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR2352917.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="ONT86_002153"
     CDS             4716..5195
                     /locus_tag="ONT86_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCR2352918.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="ONT86_002154"
     CDS             5192..5959
                     /locus_tag="ONT86_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352919.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="ONT86_002155"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="ONT86_002155"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR2352920.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="ONT86_002156"
     CDS             complement(6862..7464)
                     /locus_tag="ONT86_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2352921.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="ONT86_002157"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="ONT86_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="ONT86_002158"
     CDS             8145..8729
                     /locus_tag="ONT86_002158"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR2352922.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="ONT86_002159"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="ONT86_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR2352923.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="ONT86_002160"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="ONT86_002160"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR2352924.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="ONT86_002161"
     CDS             10630..10809
                     /locus_tag="ONT86_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352925.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="ONT86_002162"
     CDS             10831..11856
                     /locus_tag="ONT86_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR2352926.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="ONT86_002163"
     CDS             12031..13275
                     /locus_tag="ONT86_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR2352927.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="ONT86_002164"
     CDS             13428..14375
                     /locus_tag="ONT86_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2352928.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="ONT86_002165"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="ONT86_002165"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR2352929.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="ONT86_002166"
     CDS             complement(15277..15519)
                     /locus_tag="ONT86_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352930.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="ONT86_002167"
     tRNA            15640..15712
                     /locus_tag="ONT86_002167"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="ONT86_002168"
     CDS             complement(15831..16469)
                     /locus_tag="ONT86_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR2352931.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="ONT86_002169"
     CDS             complement(16592..17224)
                     /locus_tag="ONT86_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR2352932.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17575..17874
                     /locus_tag="ONT86_002170"
     CDS             17575..17874
                     /locus_tag="ONT86_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2352933.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6179 a         3158 c         3560 g         5140 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    17401 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    17461 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    17521 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    17581 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    17641 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    17701 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    17761 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    17821 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    17881 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    17941 tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt tctttatatt
    18001 gtattttatc ctttaattaa cacaaaaaca gctagta
//
LOCUS       DAJSBN010000055        17224 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJSBN010000055 DAJSBN010000000
VERSION     DAJSBN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.055"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..369
                     /locus_tag="ONT86_002171"
     CDS             1..369
                     /locus_tag="ONT86_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR2352934.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="ONT86_002172"
     CDS             549..1490
                     /locus_tag="ONT86_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR2352935.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="ONT86_002173"
     CDS             1559..2290
                     /locus_tag="ONT86_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR2352936.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="ONT86_002174"
     CDS             2434..4614
                     /locus_tag="ONT86_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR2352937.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="ONT86_002175"
     CDS             4634..5116
                     /locus_tag="ONT86_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCR2352938.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="ONT86_002176"
     CDS             5132..5893
                     /locus_tag="ONT86_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR2352939.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="ONT86_002177"
     CDS             6024..7358
                     /locus_tag="ONT86_002177"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR2352940.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="ONT86_002178"
     CDS             7665..8150
                     /locus_tag="ONT86_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCR2352941.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="ONT86_002179"
     CDS             8143..8826
                     /locus_tag="ONT86_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR2352942.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="ONT86_002180"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="ONT86_002180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR2352943.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="ONT86_002181"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="ONT86_002181"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCR2352944.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /locus_tag="ONT86_002182"
     CDS             12442..13071
                     /locus_tag="ONT86_002182"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR2352945.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="ONT86_002183"
     CDS             13123..13455
                     /locus_tag="ONT86_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR2352946.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="ONT86_002184"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="ONT86_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCR2352947.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="ONT86_002185"
     CDS             14281..15654
                     /locus_tag="ONT86_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR2352948.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="ONT86_002186"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="ONT86_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCR2352949.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="ONT86_002187"
     CDS             16709..17149
                     /locus_tag="ONT86_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR2352950.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5875 a         3008 c         3795 g         4546 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggtagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtattactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tggg
//
LOCUS       DAJSBN010000056        16666 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJSBN010000056 DAJSBN010000000
VERSION     DAJSBN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.056"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(142..1878)
                     /locus_tag="ONT86_002188"
     CDS             complement(142..1878)
                     /locus_tag="ONT86_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCR2352951.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(1875..2579)
                     /locus_tag="ONT86_002189"
     CDS             complement(1875..2579)
                     /locus_tag="ONT86_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2352952.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            2710..3213
                     /locus_tag="ONT86_002190"
     CDS             2710..3213
                     /locus_tag="ONT86_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCR2352953.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            3173..3511
                     /locus_tag="ONT86_002191"
     CDS             3173..3511
                     /locus_tag="ONT86_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCR2352954.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            3532..4950
                     /gene="ffh"
                     /locus_tag="ONT86_002192"
     CDS             3532..4950
                     /gene="ffh"
                     /locus_tag="ONT86_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR2352955.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            5062..5640
                     /locus_tag="ONT86_002193"
     CDS             5062..5640
                     /locus_tag="ONT86_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR2352956.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            5644..6165
                     /locus_tag="ONT86_002194"
     CDS             5644..6165
                     /locus_tag="ONT86_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR2352957.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(6244..6798)
                     /locus_tag="ONT86_002195"
     CDS             complement(6244..6798)
                     /locus_tag="ONT86_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR2352958.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            7051..7326
                     /gene="rpsP"
                     /locus_tag="ONT86_002196"
     CDS             7051..7326
                     /gene="rpsP"
                     /locus_tag="ONT86_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR2352959.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            7338..7589
                     /locus_tag="ONT86_002197"
     CDS             7338..7589
                     /locus_tag="ONT86_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCR2352960.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            7714..8505
                     /locus_tag="ONT86_002198"
     CDS             7714..8505
                     /locus_tag="ONT86_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352961.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            8675..9196
                     /gene="rimM"
                     /locus_tag="ONT86_002199"
     CDS             8675..9196
                     /gene="rimM"
                     /locus_tag="ONT86_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR2352962.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            9186..9947
                     /gene="trmD"
                     /locus_tag="ONT86_002200"
     CDS             9186..9947
                     /gene="trmD"
                     /locus_tag="ONT86_002200"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR2352963.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            10083..10430
                     /gene="rplS"
                     /locus_tag="ONT86_002201"
     CDS             10083..10430
                     /gene="rplS"
                     /locus_tag="ONT86_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR2352964.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(10562..11698)
                     /locus_tag="ONT86_002202"
     CDS             complement(10562..11698)
                     /locus_tag="ONT86_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2352965.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(11814..12728)
                     /locus_tag="ONT86_002203"
     CDS             complement(11814..12728)
                     /locus_tag="ONT86_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCR2352966.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(12819..13247)
                     /locus_tag="ONT86_002204"
     CDS             complement(12819..13247)
                     /locus_tag="ONT86_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR2352967.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(13252..13626)
                     /locus_tag="ONT86_002205"
     CDS             complement(13252..13626)
                     /locus_tag="ONT86_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2352968.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            13912..14097
                     /locus_tag="ONT86_002206"
     CDS             13912..14097
                     /locus_tag="ONT86_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2352969.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            14109..14357
                     /locus_tag="ONT86_002207"
     CDS             14109..14357
                     /locus_tag="ONT86_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352970.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            14388..14567
                     /locus_tag="ONT86_002208"
     CDS             14388..14567
                     /locus_tag="ONT86_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352971.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(14610..15080)
                     /locus_tag="ONT86_002209"
     CDS             complement(14610..15080)
                     /locus_tag="ONT86_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352972.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            15167..15865
                     /locus_tag="ONT86_002210"
     CDS             15167..15865
                     /locus_tag="ONT86_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCR2352973.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            16043..16384
                     /locus_tag="ONT86_002211"
     CDS             16043..16384
                     /locus_tag="ONT86_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352974.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            16377..>16666
                     /locus_tag="ONT86_002212"
     CDS             16377..>16666
                     /locus_tag="ONT86_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR2352975.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         5484 a         2905 c         3258 g         5019 t
ORIGIN      
        1 aagttttttt gagttacgta aatggaaagt tttgtctcgc ttaatttgac aaataataga
       61 gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga tgagtcatcc aactttaaaa
      121 cgcacgagca gttgctcttt tttacatttt cggaatggta acggtgaaag tcgaacctgt
      181 tccaagatga ctgtctacag taatcgtacc attaaggagt tgcacgtagt cttttacaat
      241 cgctaaccct aatccggtcc cgcctgaatg tcggcttcgc gccttgtcta cgcggtaaaa
      301 acgctcaaag atccgttcct gatcctcaat gtcgatacct ataccaaagt cttggacaga
      361 aaacgataga tcatctgttg cctgatagcg gataataatc tcgctgtcgc tttttgaata
      421 ttggacagca ttttcaatta gatttttgat aattggataa aggatatctg ttttagtagt
      481 aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt ttcttttcga tgattggctg
      541 ataattttga acgatttgct ggaaataagg ttctgtataa attgtctgga tctcgtaaga
      601 attctcgcca tcttttgaca gctggatgat ttcccgaatc agtttatcta accggatcgc
      661 atctttttgc atgatctcaa gaaaagaagt cagagtttgc gggtcatctt ttgcaccatc
      721 cagtaaagtc tcggtaaaac caatcagtga agtcacagga gtttttaatt cgtgagagac
      781 gttgccaacg aaatcctttt gtagcttttc taaacgtcgc actttcgtta agtcatacgc
      841 cacccctaga atctgtccgg taccttctga attagtaaag tagcgcaaac tcaagtctaa
      901 tgtctgccga ccttctgtaa gggtgatctc ctgatggatc agcggatgct caggagtcac
      961 ttgatgaatc agctgaatga gttttggctc ttgaatgact tctgtgtacc gctgttcagg
     1021 ttgatagctt accacgttta aatgtagctg catctttgga ttaagcagta gtaaacgaga
     1081 atcagcatca tcaatcagga atacgccaat catcagttcg ttcagtaacg tatataattg
     1141 ctcttctgtg gtagtacacg cccgatacat cttactcatt tgttcgctca acgcattaat
     1201 cgtctgatac aattcttccc attgatcggg tgtttgcatg attacttccg cctgattagg
     1261 attttgcacc atttttttta gtacgggcag cacggttttc aatggctggt ttttttgata
     1321 gatcagataa tagatcattc ctgcaagaac aaggaagaaa gacagaaaga agaaaaagac
     1381 ccaacggcga aaactctcag ttctcggtaa aaaaccgctt gtcggttctg cgatacgtaa
     1441 aatggcttct aactggccac tttttttgac tggtaatgcg acatataaaa gatcctcatt
     1501 caaagtagta cttttgcgca aagcgctacc taggttccca ccattcaata cggctttgat
     1561 ttcgggacgg gagtttcgct gttcattcaa tgtttcattg tttgtgtcaa ataagatctt
     1621 tccagtagca tccattaaag taatccgttc gttcgactga tggacgaatt cttccaaagc
     1681 agttttgttt tgcgctgctt ggaggttttc gacatctaat tgccggatca gcagttgtgc
     1741 tttttttgtc aggtagcttt cttgctgttc cacgatctgc tgttgaaaat aatgggaaat
     1801 caattgccag ccaccaaaaa acaaactaac taaaagaata agaaagaaga gacgttcata
     1861 aatttttttc catttcatcg tttgggttcc tgaaacttat agccaaatcc tctaatagtc
     1921 accaagtact ttgggtgttt tggatctcgc tcgatttttt ctcgcaaatg actaacgtgt
     1981 acatctacga ttcggctttg tcctgcgaag tcaaaattcc aaatgcggtc tagcaatgtg
     2041 tctcggtcga tcacacggtc ttttcgtttc atgaaataaa caagtaattc aaattctttc
     2101 ggtgttagtt caatcagatg gtcgtctacg gtcacttgat aattggtcaa atcagcagtg
     2161 atttgtccaa tcgatagata ttcaggctca gcttcatctt ttgattgctt acgaggttca
     2221 attcgtctga atattgcttt catacgggct aatacttcgc gaggactaaa tggtttggtc
     2281 aaatagtcgt cagcacctat ttctaggcca atgatccgat ccacttggtc atctttagcc
     2341 gtcagcatca aaatcggtgt ttcatttttt tcttgacgca gccgctgagt gatctccatg
     2401 ccatccatcg ttggaagcat cacatctaaa atgatgaaat caaatgattg ttctaatgct
     2461 aattctaatc cttcgcctcc atctgtggca ctggttacct tgtagccttc tttttccaaa
     2521 ttgaacgtca agagcgttac aatagaaggt tcatcatcaa caactagtat ttttttcatt
     2581 caaatctcct ttaaataggt ttcattctgt ttatatttta gcatagaatc agtacaaagg
     2641 gctattttac aaaagaaata aatggtataa taggaaggca ctttgctgaa tagaaaggaa
     2701 gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc tggcctgtcg ctatgatgga
     2761 cagaaaaaag aagtaccaga attgatggaa ctagttgaaa ctggagaagc tgtcatggtc
     2821 tgtccagaag tgatcggcgg attaccaatt cctagagatc ccgctgaaat cattggtgga
     2881 aatggattcg atgtgtggaa agatcaagca aaagtatgga cgacaagcgg cgaagatgta
     2941 acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa aattgcagga aaaacacatt
     3001 gatctagtca tcatgaagga aaaaagccct tcatgcggaa cccattcgat ttatgacgga
     3061 agtttttccg gaataaaaaa aaatggtgcc ggagtggcta ctgcttattt tattcagcaa
     3121 aaaatgactg tgctctctga tgaagaatgg ctgaaaacta gaggtgagca gaatggaaat
     3181 cgaaaagacc aatcgaatga acgcgctctt tgaattttat tcgaccttat tgacagaaaa
     3241 acaaatgaac tacatggaat tatattatgc agatgatttt tcattaggtg agatcgcgga
     3301 agaatacgaa gtaagccgac aagcagtata tgacaatatc aagcggacaa gcaagatctt
     3361 ggaggattat gaaaaaaaac tccatctttt ttctaattat attgttcgag aacaggtatt
     3421 ggaaaatatg actgcatata ttacagataa gtacccggaa gataaaaaac tacttgaata
     3481 tgtacagcaa attcaagcaa tcgaagaata aataaaagga atgaagaaaa tatggcattt
     3541 gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa taagaaaaaa aggaaaagta
     3601 tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc ttgccttact agaagccgat
     3661 gttaacttgc aagtagtcaa agattttact aagcgcgtac gcgaacgtgc ggtaggggca
     3721 gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa aaatcgttga tgaagaattg
     3781 acgatcacac ttggttctga aacagcagag ttaaataaat caccaaaaat accaactgtt
     3841 atcatgatgg ctggtcttca aggggcaggt aaaacgacct ttactggtaa attagccaat
     3901 tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg caggggacgt atatcgacca
     3961 gctgcgatcg accaattgaa agtattaggt caacagctag acgttcctgt atttgatatg
     4021 ggaactgaag tcagcccggt agagatcgtc cgtcaaggga tggagttagc aaaagaaaag
     4081 aaaaacgact atgtattgat cgatacggca ggacgtctgc atatcgatga aacattgatg
     4141 gatgagttga aacaaatcaa agaattgacg caaccaaatg agattttgct tgttgtcgat
     4201 gccatgactg gtcaagatgc cgtcaacgta gctgatagct tcaaccagca attaggtatt
     4261 acaggggtcg tcatcactaa acttgatggg gatactcgag gcggggcagc actatcgatc
     4321 cgctcagtga caggcgcacc aatcaaattc atcggttctg gtgaaaaatt gactgactta
     4381 gaagtattcc acccagatcg tatggctagc cgtatccttg gtatggggga tatgctgaca
     4441 ttgattgaaa aagcgcagca agactacgac gagaaaaaag cggaagaact tgcacagaaa
     4501 atgcgggaaa attcattcga tttcaatgac tttattgaac agcttgatca agtgatgggt
     4561 atgggcccac tggaagatct aatcaaaatg atcccaggaa tgaatcaagt ccctggaatc
     4621 gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa aagcgatggt ttattctatg
     4681 acgccagcag aaagagcaaa tcctgatctg ttgaacccaa gccgtcgtcg ccgaattgct
     4741 gccggttctg gaaacagtgt tgttgaagtg aatcgaatga tcaaacaatt caaggaatca
     4801 agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca tcccaggtat ggaccaaatg
     4861 tttggttcag gcgtcaaagg aaaactaggc aaaatggcga tgaatcgaat gatcaagaaa
     4921 aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa acagcttcgg tacagactga
     4981 aaatgtctac tgccggagct tttctatcag caatttttat tctttactaa tacaattact
     5041 ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg aacaaacaga aagtatgcgg
     5101 caatatcatg atactgtctg gggtgttcct gaatatgatg accaaaagct gttccgaaaa
     5161 ttgatgcttg atattaatca agccggattg agctggcaga cgattttaaa taaaagtgaa
     5221 gcattcgatg aagcatatga tcgattcgat atcaaaaaag tcgcaaatta tggagaaaca
     5281 aaaatcagtg aactaatgga aaacaaagga attattcgta accgaagaaa aatcgaagca
     5341 gccgtccaaa acgccaaagc agtcttgaaa attcaagaag aaatcggcag tttttcagag
     5401 tatctttggt cgttttctaa tggaaaaatc gttgattctt cttatcattc acaagaagaa
     5461 gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag atctaaagaa aagaggattt
     5521 aaatttatcg gaagcacaac aatctatgct tttttagaag ctgtcgggat catcaatgat
     5581 caccttgaca gttgtttccg caaagaagag gtcgtacgta tcggcagaaa gggcgaataa
     5641 agcatgcgaa aaatcatatt ttatggtgct ataagtttag atggctattt ggcaacgaaa
     5701 acggatgatc ttcaatggct ttttgacact ccaacaggta agcaaactac ttatgaagct
     5761 ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa cttatcagga agcaaaaaaa
     5821 tatttggaga ccgaagcaat ttatccagag aaaaagaatt tcgttttttc cacccacgca
     5881 caattacaat tgccagatgc tgaggtcgta aacgaagatc ctgttgcttt tctaaaaaat
     5941 ctacaaatga cagaaggaaa agatatctgg gttgtgggtg gcggtcgatt gatcaaaccg
     6001 ctattggaac atcaaatgat tgatgaatgg tatatccaga ttacccctgt attattaggg
     6061 gacgggattc ctttattcca aaaaggaaca tatgaagcgc gttttgaaat gctagacacg
     6121 acacgattcg gtgaatttat cgaattgcat tatgtacgaa agtagaagta aataaaataa
     6181 aaaaggcaac tatatgcaga aatgtcctgt acatagttgc cttttttatt accgttatgc
     6241 tttttactct gttaatttgt tagaaaaatc tagatacgca tctaaaatcg tttggaagaa
     6301 acttaatgtt ccttcaacga tattaccatt ttcatcaatt agattcgcaa cgtttccgat
     6361 atatgcttcc ggttgctgca aggttggcac attcaaaaag acaagtgatt ggcgtagatg
     6421 gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca gtaacgacta aacctggttt
     6481 atgatcccaa acactgtgtc cataaggtct tgagcctaca tctaaagcat ttttcaatac
     6541 tgctggcaca gaacggttgt attcaggagt aacgaaaacg acaccgtcta gtcccctcac
     6601 ttcctcacga aaacgagtcc attcagcagg agcctgttct ggagtttcga gatcctcatt
     6661 gtaaaatggc agatcatcga tttttacaaa tacactttcg tacccttcag gaagcaattt
     6721 cgcaaaagct tctgctactt ttttgttgta agaatctttt cttaaactac caataaaaaa
     6781 tccaattttt tttgtcatac gatttttcct ccttcaaact atctataata atcttacaaa
     6841 agataagtaa ctggagcaaa taaaaacact tactgacaaa agctacatag aagatagatt
     6901 acttattttt aacaaaaaag aagtctaaaa tcttctattt tctaaaaaaa atcgaattta
     6961 atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa aactgttaca ctacatcttg
     7021 tgaataaaac aatggaggtg gaaataaata atggcagtta aaatccgttt aaaacgtatg
     7081 ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg attcacgttc tcctcgtgat
     7141 ggacgtttca tcgaaactgt aggtacttac aatcctttga aagaccctgc agaagtagtt
     7201 ttaaaagaag acttagttct tgactggtta tcaaaaggtg cacaaccttc tgacactgta
     7261 cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc acgaagctaa attctcaaag
     7321 aaataaggtg accggatatg aaagatttaa gcgacttagt cttaacaatc gttcgtccgc
     7381 tagtcactta tcctgatcaa gtcaagttag acgtcgtaga gtcgaaagac ttttacgagt
     7441 acaatctaac agtggctcct gaagatattg gccgtatcat tggaaaacaa gggcgtgttg
     7501 ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa tgcaccgaaa aaagtgcgtt
     7561 tgaatatcat cgatgataag gaaaagtaaa taaacgtgct gtactcgaat tttcgagtgc
     7621 agcttttttg ttttttttat taattgaaga aatgattatt tggcaaaaca agcaagaaag
     7681 cgtatcctta taggaggagg gagtgaacag ccaatgagaa agaaaatgtt atttattcca
     7741 tttgctctat tgttaagcgc ttgtagttcg gttactcaag aatcagacaa tacacatcta
     7801 tcaagcagta tatctgaaac tgttaccagt ccagaaaaaa gtagtaccaa aacggcttcc
     7861 accactgaac aaacaatggc tacctcaaat aatgagaaaa agactgcttt agaccagttg
     7921 aaagaacaac agccaaatgt tcccatgcct ctagatgtac cagtctcttc aggctatctc
     7981 aatatcgcag cgacacatac aaagcaaggt tattctattt tatattacag aacagatcgt
     8041 cccctcgggt tgaatgctga cgaattgaat caagaaacgc ctattgcaac ctatctatat
     8101 caatatgggt ttgcttctag tcaagaaaca atccaagttt tgcagccatt tgagatcgac
     8161 acaaatggtc agcaagtgga cttaggttct cgaatcacag ggtatcagca aggtgcagca
     8221 ggttcaagtt ttcttgaatg gcaagaagga aactggcgta ttcgaatccg tgggaataat
     8281 atagaaggcc aagatccctt actattagct aaagaaatcg tagcttattt ggaagaaaac
     8341 agtctaccag cacctgaaca atttggtaaa attactgttg atatgggtga tacgacgaat
     8401 cgagccgtag aagtaagctg gcaagaaccg aaaaatgcct atacgatcac tcatcaagat
     8461 cctatgtctg cgttgaagat ggcagtttct atgaagaggt tgtgaaatca gcgcgctgat
     8521 ctgagcccat catttaactc caagtcacag gaagtaatca aaaatagcac gccgcatttt
     8581 tgcataattt tggcttaata ccttaaaaga aaactagaat tgcctcttca cacatgctaa
     8641 aataaaacag acaaaaaaga aatggggata taagttgact gaatacttga atgtaggaaa
     8701 aatcgtaaac acacaaggta tcaaaggcga agtccgtgtt atctcaacaa cggatttccc
     8761 agaagaacgt tacaaaaaag gaacggtgct aacactattc caagacggaa aagcacctgt
     8821 ggaattaaca gtcaaaagtc atcgcaaaca taaaaatttt gacttattga gttttgaagg
     8881 acatccttct atcaatgatg tggaaaaata tcgggatggc atattaaaag tgtcaaaaga
     8941 caactctgta gatctagctg aaaatgaatt ctattaccat gagatcatcg gtctgcatgt
     9001 attagaagat ggaactgagt taggtaaagt aaaagaaatc ttgtcaccag gtgccaatga
     9061 tgtttgggtc gttcaacgtc cgaaaaaagc tgatatattg cttccatata tcgcttctgt
     9121 cgtcaaagaa gtcgatttag aagctggagt ggtccgtgta gaaattccag aaggactgat
     9181 tgacgatgaa gattgatgta ttgactttat ttccaagaat gtttgaaggt cctatggggg
     9241 aatccatcat cggtaaggct gtagataaag gattattaga tatcaatatc tctaacttcc
     9301 gcgattattc agataacaaa catcagacag tagatgatta tccatacggc ggtggagcag
     9361 gtatgctgct caaagtccag cccatctatg ataacatcca agccattgaa aaagctgcac
     9421 cggatgtcaa aaaaagagta attcttcttg acccagctgg taaacgattc gatcagaaaa
     9481 tggcagaaga attttcacaa gaagaacact tagtattcat ttgtggtcat tatgagggat
     9541 atgatgaacg tatccgcaca ttagtgacag atgaagtttc cttaggagat tatgtcctta
     9601 ctgggggaga attaggtgcg atggtcatga tcgatgcaac agttcggctt ttgccagatg
     9661 tgttagggaa tcagacttca gctcaaacgg attcttattc aaccggttta ttggaacatc
     9721 cccaatatac tcgcccggca gaatataaag ggatgaaagt accagaagta ttgacaaatg
     9781 gaaatcataa attgattgaa gaatggcaat taaaagaatc actaagaagg acctatcaac
     9841 gtcggcctga tcttttggaa acacttgaat ggacacctca aatgacgaaa tttttagaag
     9901 aaatcaagaa agaagaacaa gaaaaaacag cagatgaagc caagtgactt tttctaaaat
     9961 cttctttaca gtaggttgat aatatgttag tattttatgg tgagtgcaaa agcacttaac
    10021 tattacgata ttccgctgtg agaaaagctc ataagaatat ttggaataag gagaagaaaa
    10081 ccatgaatcc attaatcgaa gaattaacaa aagaacaatt acgttctgac atcccagcgt
    10141 tccgccctgg tgacactgtg cgtgttcacg caaaagttgt tgaaggtact cgtgaacgta
    10201 tccagttatt tgaaggtgtt gttatcaaac gccgcggtgc tggaatcagc gaaacttata
    10261 cagtacgtaa agtttctaac ggagtgggcg ttgaacgtac attcccattg catacaccac
    10321 gtgttgcaaa aatcgaagta gttcgctacg gtaaagtacg tcgtgcaaaa ttgtactacc
    10381 tacgtgcatt acacggaaaa gctgctcgta tcaaagaaat ccgtcgttaa tcatcgttat
    10441 cagactgaga aagaaccctt ggtattcaag ggttcttttt ttgttctctg tttttttata
    10501 ttaacgattt ctatttggga catttttgat taataaaaag gggcaaaaaa ggggcaacaa
    10561 gttacagttt ttctatatct tctttcaatt tttcttttac tccctttgtc acatgcaaat
    10621 aaatcttttc agtgatatca ctattttcgt gcccaacgcg atcctgaata gcatacagag
    10681 gagttcctag ttccgctaat tttgaaatat gggtatgtct aaaaatatgt gaactaagtt
    10741 ttttatcaat cttcatgtca gctttatgat ttctcaaata ggtgttaatt gctgttagtt
    10801 ggaaaggagt tcctttcgtt gtttgaaata gaaattgacc attcggattt aattctagaa
    10861 gctcattgta aatagctatc gctttttttg gtaaatcaat ttctcgcatt ccagcagcag
    10921 tttttgtaga atcagatttt ttcatatcag ctattgaacg ttctctatac atcatcgtcc
    10981 catttataac tactgatgca ttattattgg taatgtgtac atcgtcttta cttaatgcga
    11041 ttgcttctcc aggcctcatt ccagtcaaat atagccactg gaaaaggaga gaatatcttt
    11101 tattgtgtga agttgtgaaa tttactagtc ttttatattc atcatcttct aaaaacttat
    11161 ctttaatttt tatcgttttt gactctcgtt tataatcaat aactacttcg ttgataggat
    11221 ttttttctac atagcctttt ttcatagcgt atgaaaaaag aagattcaat ttggatttaa
    11281 ttacactgac gtacttattt gacaagtcat ctttatatat catatcttca aaaatattat
    11341 ttaaatctac tgtattaata ccagaaacga tgtaagtctc aggtatcttt ttctttatag
    11401 tgtttaagat attattcgta ggataatatg tagactcctt aacttgtctt ttgtaaataa
    11461 ctaaccattc ttcgacaagc tcatgaaatg ttagatcagg cttctgaagt gttttttcgt
    11521 ttaattttat atcaattttt ttatttaact ctagcaatgc cactttctga gtttctcgag
    11581 atttattttt ataagtaatg ctcacttttt ttctttttct agtttttgga tcaatatatc
    11641 gctcattata tttatatact ttttttccgt ttttatcagt tttggtttca atccacattg
    11701 ttttcatctc ctatttgttg ctataatagg aatggataag taagccaatt atagcaggtt
    11761 tatttttcat cacgtccaca aactttggcg agggaggggg cgtgtttttt tgtttagtag
    11821 ctacctttcc atgaagtatc tgcgaagaac acttcaaatg ttgttccatc atttttcact
    11881 tcaaaatatg ctgttccttg tgcggattta cccggttgga tggtttccga aaagtaatct
    11941 ctactagcta aatcagattt aatatctgat gaatcataaa attctaaata acttgaactt
    12001 gcatcaaaag gtgtagtccc tgtgttttct acaagaaaat ctacctttgc gtaaaatagg
    12061 ccttctggtt tatagaaatc atcaccgtaa tcttttgata cagactggac agttacgtta
    12121 atagagttac cattttcaga agtaaaagga actgcttctc ctatactatg tgcaactgat
    12181 gccgtctggg gttctgaaga cgaagttgta gttgtagttg tagtagagct tgaatctttg
    12241 gattttccat ctttatctaa gaaagttcgt tgattataat atacaacctg ttcagttttt
    12301 ggactaaccc aaattaacat tgtttcatca tcagaatcag ttgaataagt atattggagc
    12361 atctctaatt tttttatatc ttttgcacta tttccatgtt caattaattc agagtgctct
    12421 ttataataat ctccttgtgc atcctttgaa atagatacaa ttaattcatc ccgtgtgata
    12481 gcattgtcta attctttgta aacaaaatca tttgtccatt tacgtaacgg tgaaccaagt
    12541 gtctgataaa cttgagttaa atcatcacca acttttattt tatgaacgtc tgatgaagtt
    12601 agtttttttg tttcatattc agatgttgat gaaacagtat tttctttagt gctagcagaa
    12661 ttaggactag tggagcatcc gcttaatacc aagcctaaaa aaagcaaccc aaaaacaatt
    12721 tttttcattt ttaattcctc acttctatga tatgattttt ttgtaggatc ttagaaataa
    12781 ggtttttagt ccgtgttgca gcacggactt tttttacatt atataagaaa gcgataagcg
    12841 ctttctggaa gtccgtaaag attctttaat tcctcaattc gtttaggata ttgatcattg
    12901 tcttctttgt atagagaaac aatgagatta gcagcaaagc aattagcttc gctttcagat
    12961 ttgcttctag atgttcttgt tgatacatag taactggata agccacgatg aaaaatagcg
    13021 tgacctaatt cgtgagcgca aatgtagaat ctttcctcag agtctctcag ttcatcattt
    13081 aagaagatta ttgcacgacc tctaatttct tgaaactgtc ctttgggatt ctcgataaaa
    13141 ggaacgtatt gaattttaat gcccatcttt tcacaaatat aaaaaggatt agcggactgg
    13201 tatttccgct tcaactcctc gactaaatta atcgtatcca tctccataag ctcacatctt
    13261 tttgcctttt tctttgtctt ctttcacaat atcccagaag gttccaataa ggatatcttt
    13321 tacgcgttgg acctgttcag gcgtcagtgt ttctccgcca taagacatat tgacatttga
    13381 atctagtaat ttatcaagtt caactacttc atcttttgtt gcccagtcag gaacttggtt
    13441 tctaccgagt aaataatcag tagttacatt aaaataatct gctacaagtt gtagtctttt
    13501 agtactagga gttctatttt tccattgata aatcgtgttt ttaggtatat ttaattcttc
    13561 ttccaattga gttatactta tgcctctttt atgagctaat tcctttattc tatctagtaa
    13621 attcattttc gcacctcaca agctacgaaa acactaataa aaaaattagt taaaggtgtt
    13681 gacaactaat aaaagtgtta gtatactgtt ttcgtaagct aaaatattag ctaaaaagac
    13741 ttgataacta ataaacactt cacggtcggc aaactctgaa atgtaaatta ctaggcattt
    13801 ctgtgtctta tttagctatg tctatatact aataaaaata ttagttattg tcaacgaata
    13861 ttagctaatt ttttagctta cagattattt ttttagaaag gagctatttt tatgtctgag
    13921 aatttagact taaaaattcg agcggagatg agaaaaagaa gaatgacttt caaagaacta
    13981 gctgcgctta tcggtatttc aggagcttat ttatcagata ttctaaacgg caatcgtgat
    14041 ggaaagaaag cacaacagca tatcgaaaca gtgaaaaaaa tattggacat ccgatagggg
    14101 ggtaggcgat gacaaaacta aaaaaacaag attttgtaaa aaaatacaat tattctccat
    14161 ctacttatca acgtcgaatg tcggaactaa aaaatacagc aattttctca gcggcgtatg
    14221 aacgggtcac aggacaagaa gtttggatca atacagaatt atacgataaa tttttgtctt
    14281 tcaaatccta taacaggtta cgcacaagaa aggtaacacc taaagaattt atcgagaagc
    14341 atttagttga tttataaaaa acagaacatt ttgagaggtg aaggttaatg ggtaaattca
    14401 acagagcatt ggtattcagc gcaccgctaa tcatctacgc tttaggactt tggggaagca
    14461 ggcaagcgtt gataggaacg attgtttaca tggtttggat ttttatgggg cttgatgaag
    14521 ctgaagctga gtacagagcg aaaaagccaa ccgagggggc tgactaaaaa tgaatagaaa
    14581 agagaaacta gaatgtatat tactattact cagtttaatt ctttcactaa tttctctatt
    14641 gggaagtttg tatttttgat atcaacaatt ttgtggattt gggttctatt tagagtaaat
    14701 atcatttttg tttggtgatt aaggagttta ttaaaattaa ttttccctac ttcaaatgca
    14761 aggtaacaag atatagcact tttaggagga atatttagcg gtacagcttc agagtatatt
    14821 ctattatcat cgactgatcc tcctttagca attaatttct taaatggagt agctgaccat
    14881 ctgcggttta agttagcgga tgaaagttct aaattaacta acatagctgg ttctgttgaa
    14941 tagttagaaa tgattacttt tgtataaatc atatctgcag cgaagtaaga agcattaagt
    15001 tcaacttcta gttgagggcg cattcctttt atttttatcc ctgttaaaaa cgtactcaat
    15061 ataaaactta ttattgacat ccattgaaaa attgttaagt gaaaaaaatt caaaataaac
    15121 accaccagtt tttaactaaa ttataccaaa aaggagagaa gaaataatgc aagaattagt
    15181 aattttgaaa aataaagaag ctgtgactac gagcttacaa gtggcagaaa gcttcgagaa
    15241 aaaacatcaa catgttttaa gagatattga tgcactaaaa aaagatgtgt ccaattttgg
    15301 acagatgttt gtagaaggta atgaaccaga ttcatatggc agaaatcgac gagttttctt
    15361 cattagtaga gatggatttt tcttgctggc tatggggttt acaggaaaga aagctatcta
    15421 cttcaaacaa aaatacattg aagcattcaa cgaaatggaa gatgttattc gcaagaatac
    15481 tgttcctcaa acaattgaag atatgatgat ctatcaatta gaagaaatga aagatgttaa
    15541 aaaagatgtt tccatgctta aagatactat gcgaattagc ggacaacaag agtttgaaat
    15601 taagcaaaaa ggaaatatga aagttatggg agttctaggg ggaaaagaaa gccgagctta
    15661 tgaagaaatc agcaaaaaag tattctcaaa attttggtct gaatttaaac gtaccttttc
    15721 aatcccaaga tatggcgagt tacctcgtaa gagattcgat gatgctgttt catttattga
    15781 aatgtggttg ccagaaactg cgatccgtat ggaaattgat caactgaaca gacaacagag
    15841 actttttggt gatgacaatg aatagagctg aagcgctaag aatagggacg gcaattgcta
    15901 attgctggtg gaaatactat aaaccaatca tcctaagcca acaacatatt gacaagcaaa
    15961 aagcatggca acaaataaaa aagtgactcc gccggcaagc aaagagtcac aaagaaaaca
    16021 catcataagg agattttagc atatggaaaa agaactttcc actctagatc aatatttgat
    16081 tgatcctgat tggggcaagc cgaaaattga ggaaacaagt ggtcgaaaaa tcagacgaaa
    16141 tcttttgacg aatgaagaac tagcttggga tcaagatgat ttaggcaacc atgtaactat
    16201 ttgggatcat gtttatctta tccatctatc gaagcattcg aataaacctg aatatattta
    16261 cgtcatcgaa gatggcttga ttgatgcgct agaagagtac gacagagata acttgattga
    16321 tatctcttat tacggaccag gtaagaaata cattgctgaa atggaggcag aatttgatga
    16381 gtgaaatcaa agggacaacg aactttgaaa aactttttag tcgtaagtta aataaaattc
    16441 tcaagaaaaa aggaaatttt gattatttat cttgggctca cgcgtgggag attatgaaaa
    16501 agaatgatcc acaggcaacg gtaactatta atgagtacaa acactacagg gttgtttctg
    16561 gaactcatca agactttctt gttgaggaat ataaaccttt tcttatggat gaaactggga
    16621 cttatgtatc tgtctcagta acggttaaag gacacacgga aaccga
//
LOCUS       DAJSBN010000057        16319 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJSBN010000057 DAJSBN010000000
VERSION     DAJSBN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.057"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..620)
                     /locus_tag="ONT86_002213"
     CDS             complement(<1..620)
                     /locus_tag="ONT86_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352976.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYP"
     gene            complement(799..1518)
                     /locus_tag="ONT86_002214"
     CDS             complement(799..1518)
                     /locus_tag="ONT86_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2352977.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1649..2800)
                     /locus_tag="ONT86_002215"
     CDS             complement(1649..2800)
                     /locus_tag="ONT86_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCR2352978.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3017..3757)
                     /locus_tag="ONT86_002216"
     CDS             complement(3017..3757)
                     /locus_tag="ONT86_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2352979.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3761..4084)
                     /gene="rsfS"
                     /locus_tag="ONT86_002217"
     CDS             complement(3761..4084)
                     /gene="rsfS"
                     /locus_tag="ONT86_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR2352980.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4141..4734)
                     /gene="yqeK"
                     /locus_tag="ONT86_002218"
     CDS             complement(4141..4734)
                     /gene="yqeK"
                     /locus_tag="ONT86_002218"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCR2352981.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4718..5368)
                     /locus_tag="ONT86_002219"
     CDS             complement(4718..5368)
                     /locus_tag="ONT86_002219"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR2352982.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5378..5689)
                     /gene="yhbY"
                     /locus_tag="ONT86_002220"
     CDS             complement(5378..5689)
                     /gene="yhbY"
                     /locus_tag="ONT86_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR2352983.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5705..6814)
                     /gene="yqeH"
                     /locus_tag="ONT86_002221"
     CDS             complement(5705..6814)
                     /gene="yqeH"
                     /locus_tag="ONT86_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCR2352984.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6811..7338)
                     /locus_tag="ONT86_002222"
     CDS             complement(6811..7338)
                     /locus_tag="ONT86_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCR2352985.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7518..8303)
                     /locus_tag="ONT86_002223"
     CDS             complement(7518..8303)
                     /locus_tag="ONT86_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR2352986.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8296..9165)
                     /locus_tag="ONT86_002224"
     CDS             complement(8296..9165)
                     /locus_tag="ONT86_002224"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCR2352987.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9166..10545)
                     /locus_tag="ONT86_002225"
     CDS             complement(9166..10545)
                     /locus_tag="ONT86_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR2352988.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10553..10972)
                     /gene="fabZ"
                     /locus_tag="ONT86_002226"
     CDS             complement(10553..10972)
                     /gene="fabZ"
                     /locus_tag="ONT86_002226"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR2352989.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10997..11473)
                     /gene="accB"
                     /locus_tag="ONT86_002227"
     CDS             complement(10997..11473)
                     /gene="accB"
                     /locus_tag="ONT86_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR2352990.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11476..12711)
                     /gene="fabF"
                     /locus_tag="ONT86_002228"
     CDS             complement(11476..12711)
                     /gene="fabF"
                     /locus_tag="ONT86_002228"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR2352991.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12730..13467)
                     /gene="fabG"
                     /locus_tag="ONT86_002229"
     CDS             complement(12730..13467)
                     /gene="fabG"
                     /locus_tag="ONT86_002229"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCR2352992.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13479..14396)
                     /gene="fabD"
                     /locus_tag="ONT86_002230"
     CDS             complement(13479..14396)
                     /gene="fabD"
                     /locus_tag="ONT86_002230"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR2352993.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14503..14727)
                     /locus_tag="ONT86_002231"
     CDS             complement(14503..14727)
                     /locus_tag="ONT86_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR2352994.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14780..15742)
                     /locus_tag="ONT86_002232"
     CDS             complement(14780..15742)
                     /locus_tag="ONT86_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCR2352995.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15752..16192)
                     /locus_tag="ONT86_002233"
     CDS             complement(15752..16192)
                     /locus_tag="ONT86_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2352996.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4300 a         3718 c         2791 g         5510 t
ORIGIN      
        1 tcaggatatt ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga
       61 tattctgggt caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa
      121 attcgattcc attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca
      181 caatccaaaa tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt
      241 ttcgtgtcta tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc
      301 cactctaata gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct
      361 gaatcagaac gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt
      421 gcgcggtcca ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct
      481 aatcgatagc cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt
      541 acgttcacag ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct
      601 aaagtgattt ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc
      661 acatgatact tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac
      721 aaaaaagcaa gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt
      781 ttcgttaaaa ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc
      841 ttctagatct tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag
      901 tgttacctcg ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt
      961 ttcttttaat gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg
     1021 tccttcttct tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat
     1081 ctcgtcagcg tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac
     1141 cgctccggaa acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc
     1201 tgttcgattc acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa
     1261 tccttcatag cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc
     1321 tcggtcaata atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag
     1381 tttttggttt gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc
     1441 aaattttgca taaatccggc tattattagc atctttagca gttttctttg ctacgatatt
     1501 tgcccattta cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa
     1561 aacgtcctgt tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc
     1621 ttcattcttc tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct
     1681 gttccggaat attgcctccc aattgataga tttgatcact tctaactaac agttccgctt
     1741 cttgtgcctc tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga
     1801 atcttcttcc agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag
     1861 gaatctcttg ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc
     1921 atgtatatct ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag
     1981 ctgctgtttt catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca
     2041 gcaaagtaga agataacaac cgataacgca aatacggcca ataagattcc caagtctgca
     2101 gcgaacaagt tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc
     2161 cttcttctaa cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt
     2221 tttcatgata gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact
     2281 tggcattttc ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg
     2341 taatatcggg atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt
     2401 gatcagacag ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc
     2461 cgaaactgct gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt
     2521 ccagtgtttg caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc
     2581 attcgacggg aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat
     2641 gccatttatc gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa
     2701 tgacgacctc tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt
     2761 gaaaaggatt gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt
     2821 atcgataaaa aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa
     2881 ctatataaac tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc
     2941 aatgcctata tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact
     3001 cgagcaggca aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg
     3061 atgctgcttc gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca
     3121 gcattcttaa ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa
     3181 taaatcgacc agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt
     3241 gtttctcgct tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat
     3301 agctatattc tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc
     3361 ggccattttc ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat
     3421 ttttcatata gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg
     3481 ataaatcgag catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct
     3541 ggctggcaat catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt
     3601 ttgcaaaggc aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt
     3661 cttcttgtaa atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca
     3721 cttcatcgta aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa
     3781 gatcgactag cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg
     3841 catggaaaac atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt
     3901 ttccttcaat acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg
     3961 taattgcgtt gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca
     4021 aggaaacatt tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt
     4081 caatcgcaat ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc
     4141 ttattttttt gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc
     4201 ttgcgcaagc aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc
     4261 agcccatgca atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata
     4321 gtctgctaca taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac
     4381 ccggattgca tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc
     4441 gccaacaaga ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc
     4501 tcttacgatt accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa
     4561 tgcagcaata ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt
     4621 ttcttctact ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg
     4681 catcaggttt tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc
     4741 cttcattttg aatataatca attactttat ctggaacaag gtagcggata gaacagcctt
     4801 ctttgatctt ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa
     4861 ttacaggata tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca
     4921 tagaagtcag ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga
     4981 taataaagta ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg
     5041 tgtaactttt cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt
     5101 ccaccgctaa ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa
     5161 catgaggtgg ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag
     5221 cttgttcagc catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc
     5281 ctacttgctt tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa
     5341 caaatgtctt agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg
     5401 atagcctttg atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga
     5461 tttggacgat ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct
     5521 catctgtatt ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa
     5581 tttgaacgat cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat
     5641 gatgcgcttt gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc
     5701 ttccttaaat caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa
     5761 ttactactgg cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt
     5821 ctttcacaga aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag
     5881 gaggctgtaa taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg
     5941 ttcgatgaat atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga
     6001 taaaatcaaa tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa
     6061 ctttaggttt gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt
     6121 gcgccatttg atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta
     6181 aaggaatctc gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt
     6241 gtactcccgc tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta
     6301 ctccgacgat atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg
     6361 tcatctcatg agatttctta gcactagtta ataatacatc tactggacga attcctgctt
     6421 cgtaagcacg ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata
     6481 tatccacttt gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg
     6541 ggattagtga accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt
     6601 gccctaattc attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttgggtct
     6661 cattataatg gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt
     6721 tttccaaagc cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg
     6781 caccacagcc gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc
     6841 agggtgtttt gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt
     6901 cttccaacta tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt
     6961 ggctccacgt atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa
     7021 atccaattgt ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac
     7081 atacaaaaga tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac
     7141 aacaacaaca gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt
     7201 tccatctgga ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat
     7261 gcctagattc tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg
     7321 tttgtaattt gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta
     7381 ctcctcaacc ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt
     7441 ctttcagaaa aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat
     7501 ttagaaacaa aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta
     7561 tgctgatcta atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt
     7621 gcgactttat atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg
     7681 attcctaact cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg
     7741 cgactaccgt ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc
     7801 gtttcttcca gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca
     7861 ccttcaccaa tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt
     7921 ctggcaatag cctctccttc gccgcgctct tctgctccta caccgcaata tgcacctgct
     7981 gtattgatca aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa
     8041 gcttttcgat agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga
     8101 tcccgtcctt tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca
     8161 ccaacaatcg cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg
     8221 aagatctctt caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg
     8281 acatcatgtg ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag
     8341 cgtatggatt ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga
     8401 taataagaat tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat
     8461 gacgcgtctt ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc
     8521 cattgcaaaa ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa
     8581 aagaccttca ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga
     8641 aaagattcct tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact
     8701 ttcctttgtt gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact
     8761 tcccatgata aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc
     8821 tttaccagtg aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc
     8881 cgtatattcg ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa
     8941 acttttttca tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc
     9001 gcaatgaggg catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt
     9061 tggacacttt gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct
     9121 attaggattg atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat
     9181 ctccgtttct ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc
     9241 acctgctatg actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat
     9301 tccttctgtg ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt
     9361 atttccatgg acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc
     9421 cgggtaaact gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg
     9481 gatcttccct ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc
     9541 gattgcgtgt ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc
     9601 tatctccacc tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc
     9661 cacttggatt cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa
     9721 ctcgatcgtt ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc
     9781 tagtgcttct ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg
     9841 attgttgcgt tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc
     9901 acctaagatc tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc
     9961 atcatttcca aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc
    10021 ttcttttgat tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat
    10081 cactggataa ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc
    10141 agtacttcct ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt
    10201 atttcccatt gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc
    10261 acacatggag gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc
    10321 ggcttttgtc acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc
    10381 ttttgccggg ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc
    10441 tgcttctgaa tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg
    10501 tacagcgatt tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa
    10561 tcataaaggt taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc
    10621 ctattccggc tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg
    10681 gtgtaacttt cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct
    10741 tgaattgttc aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca
    10801 atacccctgg cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg
    10861 ttacattttt cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca
    10921 aaagcattgg ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc
    10981 ctctttcctg ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa
    11041 ctgactcgtt ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat
    11101 tcatcagttt catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta
    11161 cttttttgaa agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg
    11221 aaacaacctc gattccatca aatactgccg attctttttc tttcattatt tctggagcag
    11281 ttgttgcttg ttcaggtatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt
    11341 gaaccatatt catgcctcga cttgttttat ttttattcat atacaattca aattgacctt
    11401 cacgcaaatc gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt
    11461 cattgatatc caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc
    11521 aaaccctaat gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac
    11581 aactgtcaag tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg
    11641 atgttctaat gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg
    11701 tccagtcatc gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat
    11761 ttggattgct tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat
    11821 atatccgact tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga
    11881 tgcaccagaa ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta
    11941 tccaacgatt tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag
    12001 aactagaacg cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat
    12061 cgaaccacga ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat
    12121 ccccatctca caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg
    12181 tttgatgtta cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat
    12241 agctgtacaa ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc
    12301 aatcgccatt ggtacgaaca ttggagaaac tcgttttgca cctttttcat gtagacgaat
    12361 aacttggttt tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg
    12421 gtcttgattc attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta
    12481 gattccatag atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc
    12541 gtaagggaaa tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt
    12601 agtgatcggt gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc
    12661 atttccaata ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct
    12721 ctccttttct tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat
    12781 atatggattt tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg
    12841 ctgcatcgga atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat
    12901 atcagttgtg atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc
    12961 tcgtgccaca gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg
    13021 accaatattt ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg
    13081 cttcatcatt tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc
    13141 cagacatttt ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc
    13201 ggcattgttt actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg
    13261 tcccgctttc tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc
    13321 ttcgatttct tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc
    13381 tccagctttt gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat
    13441 aaatacattt tttcctttta catccataac gttcctcctt atagtattgc tattgtttca
    13501 tcaaatgtct gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata
    13561 aatccgctta atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt
    13621 aacttatgga tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc
    13681 gcgattgttt ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt
    13741 tcagagaatt gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata
    13801 gctgtatgaa aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt
    13861 aatagtaaaa cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt
    13921 gtattataat ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg
    13981 atcgtttcaa tcggcgcatt catcactgca accattttcc cgcttccagc tggggcagct
    14041 tctgtcatat aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca
    14101 ttactagcta ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg
    14161 attccatgct ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt
    14221 gtatattgcg tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag
    14281 ccgagaatct cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct
    14341 tttcccatac cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt
    14401 ttctccaata tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc
    14461 ctatttcagg aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc
    14521 tacgtaagtc actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc
    14581 aaattcatct tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc
    14641 ttcttggatg tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat
    14701 gatttcttga attttttcga ataccataat aatatattcc tccgatattg tttgcttttt
    14761 atttttataa tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc
    14821 catagcctgt aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg
    14881 cttcatccaa taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg
    14941 tcaggaactt ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt
    15001 ttgcctgatg caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt
    15061 ttgcaacgtc tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg
    15121 aaccttctga atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg
    15181 agctacttct ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg
    15241 cttctaagag aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc
    15301 aatcgattag cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc
    15361 ggatcatttt ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat
    15421 caaaagcgat agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg
    15481 tcgtgtaatc gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat
    15541 ttttctgttt tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg
    15601 ttacacatct tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca
    15661 tcatcttgga aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag
    15721 taatcattcc aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca
    15781 agtttttcag tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt
    15841 tcttgaccat ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg
    15901 ttagtcccaa ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt
    15961 tttttactaa cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg
    16021 ctacttctga agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg
    16081 tgattgacag atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat
    16141 cattgaagac actaacaaga taatcattta ctgtttttaa atctggttcc atgttccacc
    16201 gtccactaat tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt
    16261 caaaatgcaa ccttaaaaga tcattttttt actatatgag ctttttttaa gattttact
//
LOCUS       DAJSBN010000058        16227 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJSBN010000058 DAJSBN010000000
VERSION     DAJSBN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.058"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            138..1475
                     /locus_tag="ONT86_002234"
     CDS             138..1475
                     /locus_tag="ONT86_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCR2352997.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="ONT86_002235"
     CDS             1523..3652
                     /locus_tag="ONT86_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCR2352998.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="ONT86_002236"
     CDS             3627..4316
                     /locus_tag="ONT86_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2352999.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="ONT86_002237"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="ONT86_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5713)
                     /locus_tag="ONT86_002238"
     CDS             complement(5546..5713)
                     /locus_tag="ONT86_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353000.1"
                     /translation="MYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKESEAFAKRIGG
                     RVKPIQEVRPD"
     gene            complement(5792..6232)
                     /locus_tag="ONT86_002239"
     CDS             complement(5792..6232)
                     /locus_tag="ONT86_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCR2353001.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6236..7081)
                     /locus_tag="ONT86_002240"
     CDS             complement(6236..7081)
                     /locus_tag="ONT86_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCR2353002.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7230..8648
                     /gene="pepV"
                     /locus_tag="ONT86_002241"
     CDS             7230..8648
                     /gene="pepV"
                     /locus_tag="ONT86_002241"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCR2353003.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8705..9652)
                     /locus_tag="ONT86_002242"
     CDS             complement(8705..9652)
                     /locus_tag="ONT86_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2353004.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9875..10225)
                     /locus_tag="ONT86_002243"
     CDS             complement(9875..10225)
                     /locus_tag="ONT86_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR2353005.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10425..11504
                     /gene="pepA"
                     /locus_tag="ONT86_002244"
     CDS             10425..11504
                     /gene="pepA"
                     /locus_tag="ONT86_002244"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCR2353006.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11648..11968
                     /locus_tag="ONT86_002245"
     CDS             11648..11968
                     /locus_tag="ONT86_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2353007.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11990..12454
                     /locus_tag="ONT86_002246"
     CDS             11990..12454
                     /locus_tag="ONT86_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2353008.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12659..13264
                     /locus_tag="ONT86_002247"
     CDS             12659..13264
                     /locus_tag="ONT86_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCR2353009.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13303..14592)
                     /locus_tag="ONT86_002248"
     CDS             complement(13303..14592)
                     /locus_tag="ONT86_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCR2353010.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15020..15499
                     /gene="rlmH"
                     /locus_tag="ONT86_002249"
     CDS             15020..15499
                     /gene="rlmH"
                     /locus_tag="ONT86_002249"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR2353011.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15702..16227
                     /locus_tag="ONT86_002250"
                     /pseudo
     CDS             15702..16227
                     /locus_tag="ONT86_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336126.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         5135 a         2971 c         3297 g         4824 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catatgggga tcacctgctt ttttcttttc attatacgaa ctactcgaca
     5761 caatacaaga aaaatgctta gacggtcttt attaagaatt tacatcatcc aaagaagcgt
     5821 acgcttggaa taactgatct aactccggat tgactttgct cagcttcact aatcgttgcg
     5881 aatcagaaag taaaaaacag tgtttactac tgccagtagt ttttaatttt ttgtcttcag
     5941 ttcccacaat ttcgtagcta aaatccaacc gtgtccccgt atacttttgg atagttggat
     6001 ggatgctcac tttttctccg taatggatca tttctttata gctgcaattt acttccagaa
     6061 cggggatgat gatcccagat tcttcgattt ttttataggg aaaccctaga tgtaccaata
     6121 aggctacccg agcctcttca aaccagcgga tataattgct gtgatgaata atccccattt
     6181 gatctgtttc ataataaaaa ggttctcgca cataacctgt aaatttctcc atgactcatg
     6241 cctcgttttc cattgatttt ttacaaaaat aattcatcca tttcggaagt gcttcgctga
     6301 tttgtgtcgg caaaacaata tattgttctt ttgccagctg gtctccaatg aaactatgaa
     6361 gataaatagc tgctgcaatc gtttggtcat tttgaggaaa ctgggctaaa aatcctgtga
     6421 tcatcccagc taaagtgtca cccatgccgc ccgttgccat agcagcagaa cctaaaggat
     6481 tggtgtagaa gtgctggtga ccataaattt ttgtctcatg acttttcaaa acgagcacag
     6541 caccgatttc ctgttgcttc tttttgtttt ctttatcccg ttgttcttgt atcggtagag
     6601 aactcaaccg ttgccactcc atttgatgag gtgtaagtac agtttgttcg ggatagggta
     6661 gcaaaagatg attagcagca agtaaactta aagcagagcc atcaatcacc agccattgtt
     6721 cttttgattg gttttctagt acttgtttga aacgttccaa actcttagaa gaagtaccca
     6781 atccgggacc aatcaagagt acatctgctt gctttattac ggaaagaaac tggtcagtat
     6841 ccgtccaaac tacaatcatt gcttctggca gatgactgtg aagagcactc cgattttttt
     6901 catcagttac tacagtcgtg agacccgctc cgctgtgtac ggcagctaaa gcactcataa
     6961 tgattgctcc gccatattgt tcattgccgc caatcaaaac gatacgtcca aatgttcctt
     7021 tatgtgaaat ttttggacga ggtgtgatga ctcttttcac tagttgttcg tttatcagca
     7081 tctaaaaacc tccttttact cattatagcg tatatttttt ttagagaaaa acagataaga
     7141 aaaggaattg tcagattttt tgaactattt ttttctgtaa tttgtggtat catgaacacg
     7201 aagacttaaa taagtggagg attaacttta tgacaattga ttggcaaaaa gaagtagaag
     7261 cacgtaaaga agacttgctt gaagacttga aaaatctttt acgtgtcaac agcgagcgcg
     7321 atgactcaaa agtaacacca gatgcaccat ttggacctgg acctcgcgac gcattgaaac
     7381 atatgttagc ttatggcgaa cgtgatggat ttaaagtaaa aaatgtcgat aactatgcag
     7441 ggcatattga tcttggcgaa ggagacgaaa cgctagggat atttggacac atggatgtcg
     7501 ttcctgcagg agatggctgg gacacagatc cttatgaacc agtaatcaag gatggaaaga
     7561 tctttgcacg cggatcaagc gatgacaaag gtccaagtat ggcagcttac tatgcgatga
     7621 agatcattaa agaacttgac ttgaaactat caaaaaaagt tcgcttcgta gtcggcagcg
     7681 atgaagaaag cggctggggc gatatggctt attatttcga acatgaagaa gaaccagatt
     7741 ttggtttctc accagatgct gaattcccaa tcatcaatgg tgaaaaagga aatgtttctt
     7801 tagctttacg cttcaaaggc gacaatgcag gagattatgt cttgaaatct tttgtttccg
     7861 gacttcgcga aaatatggtg ccaggaacag caactgcagc acttcaagta ccaagtgcag
     7921 atgcagctat cgcgatggaa gaagcatttt accaattcat cgaagcaaat ccagtaagcg
     7981 gaacgattga agcggacaat acgtatgtaa aaatcgaatt agttggtaaa ggcgcacatg
     8041 gtgcaagccc acaatctggt atcaatgcag gttctttctt ggcattgttc ttagataact
     8101 atgaattcct aggatctgct aaacaattta tccatgtagc agctgcttat gtccatgaag
     8161 atttttatgg agaaaaacta ggagtcgctt atgaagatga aaaaatgggc aaattgacaa
     8221 tgaatgctgg actatttgct tttgaagaaa atggtacaga ggaagcgaac ttcatcaata
     8281 tgaatttccg ttttccaaaa ggtgtaacag tagacggatt gcaaagtgac atcgaacaaa
     8341 cagtggggca agaaggcgca acagttactc gaggtgctcg cgtaatggaa ccacattacg
     8401 taccaatgga tgatccattg gtggctacat tattgcaagt ctatgaagac cacactggag
     8461 aaaaaggcta cgaacaaatc atcggtggcg gaacttacgg acgtttgttg aaacgtggcg
     8521 ttgcttatgg agcgatgttc ccaggatata ctgatacaat gcatcaagca aacgaattta
     8581 tgagtcttga tgacttgttc agagcaacag caatctatgc agatgccatc tatcgtttgg
     8641 cgaaataatt aattaaaaag aggatcaaga attgcaaatt atttttgcag tcttgatcct
     8701 ttttttactt tctactgtgg tattcatgat aaacatgata tagattcaat tcatattctt
     8761 tcttagcgtt cgtgacgact gtatccaaaa tcaaccctgt tactaatgaa agaagtgcta
     8821 aagtcatcaa tccagtactt aaaatcgcag aagggatgcg tgtaataaag cttgtcagca
     8881 tatattcgcg aatcacagga atccccgtga ccaatccaaa taaaaagaag aagatggtcc
     8941 aaatcccaaa gaaaagcaaa ggcttataat ctttaaacat tttaataatc atcatgatca
     9001 ctttgaatcc gtcagagaat gtattcaatt ttgattcgct gccttcagga cgatcacgat
     9061 aatcgattgg gatttcaacg aacttgaatt ttttatctaa agcatggatc gtcaattccg
     9121 tttcgatttg aaaaccagaa ctcataatag gaaaactttt gacaaagata cgattgaatc
     9181 ctcgatagcc ggtcatgaca tcaagaattt ttgttttata cattctagta attgtatttc
     9241 taacaaggtt atttccaaaa tcgtggaaag gtctcttatt ctcatcaaag taggttccat
     9301 ttgataggcg atcaccaatg accatatctg cttcaccaga acgtaatttc tcgagcaaac
     9361 catgtacagc ttctgcagga taagtatcat cgccatctac cattaaataa tagtcggcat
     9421 cgatatcaaa gaacatttgg cggatcacat ttccttttcc ttgacgaggt tcttttttta
     9481 cgattgctcc accttgaacg gccaattcgt atgttcgatc ggtcgaatta ttatcataga
     9541 cataaatatc tgcttctggt aattctcgtt taaagtctgc aatgactgtt gaaattgttg
     9601 cttcctcatt gtaacaagga atcagaacag caattttttc ttgttgttcc atttttcact
     9661 ctccttaact atagaaaaca aaagctaaat taaaaatacc tcataatcaa aggaaatgct
     9721 agtttttccc gctaaatatc atttatttaa aaagaaaata ctggaatata tgtttggaaa
     9781 gctctatcta aaaaaccacc cactacaaaa gaaggacatg atacctctct tttgaatagt
     9841 gggtgcgtat tcgttctctc acttttttga cgtattaatc tgttgcacct ttttttcttg
     9901 aaatttctaa tacggttcct gtatgagcat ctgctaaaaa ttcataataa acagtttcgc
     9961 catcttctat tcgagaaaga cctccccgat aagcactcga ccgaacggcg aatttcctaa
    10021 taggttcctt ttcaaaagag atccaagaac cttcgattgc accttctttt agaaaagatg
    10081 acttgatatt ttcaagtact tggtctgctg acaatacttt tttgcgatga taccaaatcg
    10141 ttgaagctac cccgctcaat aatcctagac cgattccaac tgctaacccg cctttaaaat
    10201 agcttagttc gctttcttca tacatgtgga catacctcct tcttctcact tgttactcct
    10261 tgtgcttatt ttaccacact ctcatatttt ctttgaaata aataaccgct accagaaaaa
    10321 ataagctata ataaatacga tcggtctgca gaaaaaatca tattttatcc gcagatcaat
    10381 ataagtctga gaggagttta gtctgaaatt tagacgaata gcatatggaa gaaaaaacat
    10441 ttcaacgaat caaagagtta acagaattac aaggaacaag cggttttgaa caagatgttc
    10501 gcgcttatat ggaaagtcat atggaaccgc tggtagatga gttacagtta gatggtttgg
    10561 gcggcatttt tggtctgcgc catcatgaag aagcggatgc accccgtgta atggtcgcag
    10621 cacatatgga tgaagtcgga ttcatgctga cacaaatcca agaaaacggg ctttttaaag
    10681 tcgtacctct aggcggctgg aatccttacg ttgtttctgc tcagcgtttt acacttaaaa
    10741 cctctactgg taagaattat ccatgtattt cttcttccgt tcccccacat ttattgcgag
    10801 gaacaagcgg acaaaaatca gtagaagtaa ccgatatttt gttcgatgct gggtttgaat
    10861 caagagaaga ggcaatgagt tacggtgtcc ttccaggaga cacaatcgtt ccttacgctg
    10921 aaacgatcaa aacagccaac ggaaagaata ttatcagtaa atcatgggac aaccgctatg
    10981 gctgtacaat ggtattagaa gctttagaag cgttgcaaaa tgaaacatta ggtcatacat
    11041 tgattgcagg cgcgaatgtc caggaagaag taggcttgcg tggttctaaa gcttctgtca
    11101 ataaattcaa gccagatctg ttctttgcag tagactgctc tgcagcagat gatacagtga
    11161 cgaaaaatgg cacattcgga catttaggag aaggtacatt gatgcgtatc caagatccag
    11221 gtttgatcat gcttcctcgt ttgcgcgagt acttgctgga tatcgctgaa acaaacaata
    11281 tcccttatca atactttgtt tcaaaaggcg gtacagatgc aggtgctgct catacacaaa
    11341 acgaaggaat cccaagtaca gtgattggcg tagtgggacg ttatatccat acgcatcaaa
    11401 cgatgttcag tattcgcgat tttgaagctg cacgagaaat gttgattcaa acactgaaag
    11461 gtttggataa atcaacagta aatacgatcg tatacggaaa ataatgcgat agtgaaacta
    11521 taaaatctgg caaaagattg ctttttgcca gatttttttt ataaaaagag gatcaatgtt
    11581 tgttagttcg aaagaaaggt gtataattta attgaaaaat acataaacag aaagtagagg
    11641 acaaagaatg attatcccaa atagtttaga agaattagca ggctacgtag aaactggaaa
    11701 gagtgtattt ttctttactg ccggctggtg cggagactgc cgttttatca aaccgcaaat
    11761 gcctgaaatt gaaaatgact tttctgattg gcaatttatt gaagtagacc gtgacaagta
    11821 catagatgta gccgctgaat ggaacatttt tggtatccca agttttgtcg tgatccaaga
    11881 cggaaaagaa ttaggtcgtc tagtgaataa agatcgtaaa acaaaacagg aaatcgaatc
    11941 gttcctagaa agtgtaacaa aaggctgaca tctgttagga ggggtcaaga tgaatcaaga
    12001 atataagaat attttagtag gtatcgacgg tagtgaacaa gcacttgctg ctttcaaaaa
    12061 agcggtagaa gtggctcgcc gtaataatgg gaaagtatat gtagcaaacg taatcgatca
    12121 gcaattttat aactttatgg gctatgcacc tattgatcaa aatctagtcg atcagcaaac
    12181 tgaaacagcc aaggaaatga tcgaagaatg taaagaatat gggaaatctg ttgattacac
    12241 aaatatagaa ggaattattg catatggttc tgttcgtgaa tccatggcag tcaaattgcc
    12301 aaaaaaatat gagatcgatc taatcatggt tgggcagtca ggtctaaatg cagtcgaacg
    12361 ctttatgaca ggaagtgtag ccagctacgt gatacgtcat tcaccttgtg atgtcttgat
    12421 cgtttctgac gaagaagaac agccaagtaa ttaagcaagt ggaacttgta cgaccttttt
    12481 aatcaattaa ctaaaacagt aaagggactg tgacaaaagt cttgtcacag tcccttgtta
    12541 cgaataaatg gtgttccaga agaggcttct tcggaaataa agcatgaaaa aaatttcatg
    12601 cagtgaatgc aattttttgt taagataaat ctcagcaaag gataatggag gaaagaacat
    12661 gatttttgct tataacaaag cacacgtcgg agacaccttg atggttatta tagccgacga
    12721 taaaggaaca gaaaacactg ttgaacgcaa atggaatgtt gctcggatca aagatgaaca
    12781 aggtcaagtt gttgcttgga actttttcca tatctccgat catctaacaa tcgaagggaa
    12841 cggccaagta agcttaacag atgaggaatt agcgaaattg aatcgattga tcaaagaagc
    12901 aggattcgaa gaacagcttg aagctgatca tgaaccgaag atcgttgtcg ggtacgtaaa
    12961 aacatgcgtc ccacatcctg attctgacca tttatcgatt actgagacag aagtagacaa
    13021 tggtacagta ttgcaaatcg tttgcggtgc acccaatatc gaagctggac aaaaagttgt
    13081 cgttgcaaaa ccaggagcta tgatgccaga tggaatgatg atctggccag gaactttgcg
    13141 aggggtagat agctatggaa tgatctgttc cgctcgtgaa ttgcatttgc caaatgctcc
    13201 agcaaaaaaa ggaatcttag tattgcctga ggatgccgta gtcggagaag catttccaaa
    13261 ataaagaggt tgtgacaagt ctttgtcaca acctcttttt tattaatttt cactattttg
    13321 attttgtttt aatgccgatt gatcgatcgt caattcaaca gtagttgttt tttctttcga
    13381 gccttcatag taagtgattt tgacagaatc tcccactttt ttcttataca aagcagattg
    13441 aagttctaca ccagaagaaa cttctttgtc atcgatctta gtaatgacgt cgtattgttt
    13501 caatccagct ttttcagccg gtgtagctgt ttggacattt gtcacgatga caccgttagt
    13561 tacagatgaa ggaatcttca agatttgttc ttgttgctga gtagaaacgg cagataagtc
    13621 gaccattgtg atacctagag ctggacgtgt cacttttccg tctttttcca gttgattgat
    13681 gatattcact acatcgttac ttggaatagc aaatcccatt ccttctacgc ttacattaga
    13741 agtagattca gaagtacttg cgatcttact tgagttgata ccgataactt gtccttcgat
    13801 attaactaaa ggaccaccag aatttccagg gttgattgct gcatctgttt ggatcgcatt
    13861 gatattgact gtttcgttcg attcattcgt acttgtcact tgtcggttca aggaagaaat
    13921 gattcctgaa gtgacagaat ttgcatattc agatcctagt ggggaaccaa tcgcaatcgc
    13981 aggctcacca accttcagag cacttgagtc accaaaagag gcaactgttt cgactttgtc
    14041 tgaattgatc ttcaacaccg ctaaatctga ataagcatct gttccaacta attcagcttt
    14101 taccttcgta ccgtctttca tcaagacttc aagaccttgc tggccatcta ctacgtgatt
    14161 attcgtgact acataagcag tattcccgtc ttttttgtag atcacgccgc ttccttcact
    14221 ataagcttcc aaattactat catctgagct gctactttct tcttgctgcc cgaatagtcc
    14281 gccaaaaccg cttgattggt tttgactttg taagttgata acagaaacga cagcgtcttg
    14341 aactttatct accgctttgg tgatatcaga atctacattg actttgacat tttccacaac
    14401 tgtttcaccg gcagaattct gattgccgct tgtagcagta gaactattgt tgccagtgcc
    14461 catcgctgca taaaaaatgc cagctgtcag tagtcctcca acgatccctc caacaagccc
    14521 cagacctaac ttacgccaga ggctattatt tttacttttt ttcatctttg gtgtcacatt
    14581 ttttctatcc atgtgttcct cctccaatta cattcattta tctactgtta gtatagtctt
    14641 atttgttgac tttagtctac tttgagactg tgaacttttt atgaaaaact ttaggaaaaa
    14701 tcattgtttt cagatgaaaa tatttgattt tctttttcca cagatactgt gtaaaactac
    14761 gcggatagga ttggaaaaat aagggataaa acatatccac agcctgtgta taaagtggat
    14821 aaattcttat aaaacgtatg tttgcctaat gaatgtgatg aaataaggta aaataacact
    14881 gttttctgtg gataaacctg tggatgttgt gcataactgt tggggaaatc tttttaacaa
    14941 aaatagccaa aataaacata tagacgaaaa aattgattat aataaacaaa gtgagcatga
    15001 agaccaaagg agagaaaaca tgaatatcaa aattatctca gtaggaaaat taaaagaaaa
    15061 atacctgatc caaggtatca atgagtatgt caaacggctg aatgcctatg caaaaatcga
    15121 attgatcgaa gttcctgacg aaaaagcccc ggagaattta agcgaagcgc aaatgagaca
    15181 agtaaaagaa aaagaagggg aacggatctt agcgaaaatc aaagaacaag aatacgtcta
    15241 tgccttagca attgaaggga aaaatcctac aagtgaatct tttgctaaac aaatcgacca
    15301 attaggtatc caaggaaaaa gccaccttgt ttttgttatc ggcggctctt taggactcag
    15361 cgaagccgtc atgaaaagaa gcaatgctca gatctctttt ggaaaaatga cgtatccaca
    15421 tcaattgatg cgtctgatcc ttgtcgagca aatctaccgt gccttccgga tcaatgcggg
    15481 ggctccatac cacaaataat gatatttttt aagagaaaaa attatcccta ttttaaataa
    15541 tagcaatagg tagtggtagg gagaaattta taaataactt ttttatttgc tttggtagcc
    15601 aagtaagttg caaaagaaaa atagatactg ttaaaaagat aatgtagaca tagaaacaaa
    15661 tacgtaaaat tttagttaga ataatggcaa ggagaaaaaa catgatagat gaaaaaaagt
    15721 gtttaaaaaa tctattagag gatatggatg atattgaaga aaactatcta aatggactgg
    15781 gaaaacaaat atatttcaga tacttaagct atctaaaaat gaaataagac atagtaattt
    15841 tttagctttt ttgtttgctc caaatgaaac ccacaattta tctgatgagt tttttaaaat
    15901 gtttttgaaa aggtatatag attctaatga tgataccaaa gctgctatca attattttga
    15961 tgctttactt aatagctatg aagatttaat tgtttacaga gaaaataaga atattgatat
    16021 tttattggta tcggaaaaaa ataaaatagt ggtgtgcatc gaaaataaaa ttctgtcttc
    16081 ggagtctgga gggcaactta ataaatacca aatatatata gaacgcaatt attctaacta
    16141 taaaaaaatt tttgtttttt taactccaga tggaaatgaa cccacaaatc ctagttgggg
    16201 cataattact tacaagaata tcgttga
//
LOCUS       DAJSBN010000059        16198 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJSBN010000059 DAJSBN010000000
VERSION     DAJSBN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.059"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            207..803
                     /locus_tag="ONT86_002251"
     CDS             207..803
                     /locus_tag="ONT86_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR2353012.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(861..1343)
                     /locus_tag="ONT86_002252"
     CDS             complement(861..1343)
                     /locus_tag="ONT86_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353013.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1449..1646)
                     /locus_tag="ONT86_002253"
     CDS             complement(1449..1646)
                     /locus_tag="ONT86_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353014.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            1941..2699
                     /locus_tag="ONT86_002254"
     CDS             1941..2699
                     /locus_tag="ONT86_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCR2353015.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2759..3187
                     /locus_tag="ONT86_002255"
     CDS             2759..3187
                     /locus_tag="ONT86_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353016.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3233..3305
                     /locus_tag="ONT86_002256"
     tRNA            3233..3305
                     /locus_tag="ONT86_002256"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3266..3268,aa:Gln,seq:ctg)
     gene            complement(3455..3826)
                     /locus_tag="ONT86_002257"
     CDS             complement(3455..3826)
                     /locus_tag="ONT86_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2353017.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4041..4226
                     /locus_tag="ONT86_002258"
     CDS             4041..4226
                     /locus_tag="ONT86_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353018.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4309..4497)
                     /locus_tag="ONT86_002259"
     CDS             complement(4309..4497)
                     /locus_tag="ONT86_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353019.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4521..4784)
                     /locus_tag="ONT86_002260"
     CDS             complement(4521..4784)
                     /locus_tag="ONT86_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353020.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            4942..5199
                     /locus_tag="ONT86_002261"
     CDS             4942..5199
                     /locus_tag="ONT86_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCR2353021.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5270..5461)
                     /locus_tag="ONT86_002262"
     CDS             complement(5270..5461)
                     /locus_tag="ONT86_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353022.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5602..5922)
                     /locus_tag="ONT86_002263"
     CDS             complement(5602..5922)
                     /locus_tag="ONT86_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353023.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(5945..6379)
                     /locus_tag="ONT86_002264"
     CDS             complement(5945..6379)
                     /locus_tag="ONT86_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2353024.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6714..7352
                     /locus_tag="ONT86_002265"
     CDS             6714..7352
                     /locus_tag="ONT86_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2353025.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(7676..7759)
                     /locus_tag="ONT86_002266"
     tRNA            complement(7676..7759)
                     /locus_tag="ONT86_002266"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7723..7725),aa:Leu,seq:cag)
     gene            8287..8787
                     /locus_tag="ONT86_002267"
     CDS             8287..8787
                     /locus_tag="ONT86_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2353026.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(8977..9321)
                     /locus_tag="ONT86_002268"
     CDS             complement(8977..9321)
                     /locus_tag="ONT86_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCR2353027.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(9475..10395)
                     /locus_tag="ONT86_002269"
     CDS             complement(9475..10395)
                     /locus_tag="ONT86_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2353028.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            10582..11073
                     /locus_tag="ONT86_002270"
     CDS             10582..11073
                     /locus_tag="ONT86_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCR2353029.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(11097..13472)
                     /locus_tag="ONT86_002271"
     CDS             complement(11097..13472)
                     /locus_tag="ONT86_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR2353030.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            13798..14424
                     /locus_tag="ONT86_002272"
     CDS             13798..14424
                     /locus_tag="ONT86_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR2353031.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            14447..15151
                     /locus_tag="ONT86_002273"
     CDS             14447..15151
                     /locus_tag="ONT86_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2353032.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            15343..16176
                     /locus_tag="ONT86_002274"
     CDS             15343..16176
                     /locus_tag="ONT86_002274"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR2353033.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         4983 a         3150 c         2713 g         5352 t
ORIGIN      
        1 caaataagca aaaattccta aaacacaaca atgaaagcga taactaagaa aatccttaca
       61 taacgatttt tcttattaaa attgtttttt gaacttcgct tattttttgt gagcatatct
      121 gtttacttac tttttgtaag gtgctatact tcatttcgta atcaattaaa aaaatgcaaa
      181 acgcaaatca aaggagaaat tttattatga caacttttac tgacactttg aaaaaccgcc
      241 gttcaatcta tcatctagga cgtaatgttt cactttctaa tgaagaatta acaactttga
      301 tcaaagaagc aattaaagaa agcccaactg ctttcaatgc tcaatcaaca cgcgcagtta
      361 tccttttcgg agatgcacat gaaaaacttt gggaaatcac tgaagaagca cttcgcccat
      421 tgactccagc agaagctttc ccaaatacac aaaacaaatt agctggtttc aaaaatggtt
      481 atggtacagt attattcttc aaagatactg acgtggtaaa aggtctacaa gaacaatttg
      541 aattatatgc agacaacttc ccagattggt cagaacaatc aaacggaatc gcaacagcta
      601 atacttgggt agcattagtt gataaaggat tgggcgcaaa cttgcaacat tacaacccag
      661 ttattgacga agcagttgca aaagaatgga atatcccttc aaactggaaa ctacgttcgc
      721 aattagtatt cggttcacca gaaacaccgg ctggggaaaa agaatacatg aacgatgccg
      781 atcgcttccg cgtatttgga taattttatt tgaaataaaa aacagaggtt gtgacaaaca
      841 tcttgtcgca acctctgttt ttattctgga tcaatcataa tatcaatctc tgcctcatcg
      901 tttaagcgtt gggacaaaaa tggaactaat tcttctaatg atgcagaaaa tttcagcatc
      961 ggtcctcgcc atccgtttcg gaaaaaattc atttttacga ttttagaacc ttttggctga
     1021 tataatacaa ttttattgat ctcagaaaaa ggaacacctt ttttgtcaaa gctattcaaa
     1081 acaagtcctt cttctgtgat tcctctactg tccaacaaaa aactaataaa aatcattgct
     1141 gacaagatcc cttttgccaa ttcatctgct gtaggatgac tgatgagtgt ataccaaatg
     1201 actagtgaca ctaagaaggg tatcaccaag tagacccatc gatttcgcac agtccgataa
     1261 ataatttctt ttcgaaccag ccaaagaaat aaaaacatca caacaacgaa taaaaacaat
     1321 agaaccgtaa agaatgttac catgacaact cgcctcgctt tttcttctcc agaaaatagg
     1381 aaaagaatac tgatttacct tctcaaagaa attggacttg cagcacgccg cacgtccaat
     1441 tgttttcttt attcaaatgt atcatatttt gcaacaacca tgcttgcttt ttcatctaat
     1501 aattcttggt cctctgcagc acattgggca actttgacca cggcgcagtt tgtcattttt
     1561 gatacaactt caccaatgac cgtttttgta aaaccaattg gtttgcatgc atattgttct
     1621 cctaaattaa tcatttcttc cgtcatcaaa atcctcctaa actgctggaa cttttctcca
     1681 cattaccact gctatttctt tggagattta actcattgat ttttatttac agttaaaaat
     1741 caaccttaca ggcattatag cgtactgttt tcgttttcac aattaaaaca gctgttgtat
     1801 ttagaagaat ttttttcaaa aaattaaaac atattcctta aaaactaatt atttcctaca
     1861 tattaaaata taatacttcc atttccaata aaaatacatt tataatagga aagataaaca
     1921 gctgaaaagg gagatttcaa atgaagaaaa aaacgttcaa aaaactattg tttctagggg
     1981 gcacagctgc tcttttatta agcggtacaa gttattacct acatatccat tctccagaac
     2041 atacaatgaa cactgcacaa aaaagttcaa cacaaaaaac gaaaaagaca aaagagaaag
     2101 cagaagtttt actagatgtt ccattagaga gccagtttga ttctccttct ttagaaaatg
     2161 gctgtgaagt cacttcgctt tctatgcttt tatcttttta tggttatgaa acaactaaaa
     2221 accagttagc tgagcaattg gattacgtcc ctgttttcaa tgcagatggg actcatggcg
     2281 atcctaatga aggatttgtt ggtgatatca gtggcggaga ctgggctatg gtcgtctatg
     2341 ttcccccagt tgcctccctt gctcagcaaa tcgtccaaac ggattatcac acttttccaa
     2401 aaaccgatgc tcaactcgat gatatcaaaa aagcactatc taaaggaaat cctgtctgga
     2461 cttctgtgac tatcaatttt gaagtcccag atgaaactga ttttatgact tggaccacaa
     2521 ataatggaga agtaagagtc actcctcttg ttcatgcttg tgtagtcact ggatatgaca
     2581 aagaaaatat ttatgtcaac gatccctatg gtgtaaaaaa tcgagcagta ccgatcaacg
     2641 atttttcggc gattttcact gcaatgggtg gacagatgct aactttggag aaaagctagt
     2701 aattcaaaca aaaatccctt atactcaaga taccaaacct ttgaagggag cgtagataat
     2761 ggaaaaattc acacataaaa aaatggatcc aaatgaaatt ccgattattt ttgtccgtga
     2821 ctgcaaggga aatgttcaag gtaaagtctc tattaatgag tggaacgagc gtcgcagacc
     2881 tgctaccctc aatgaactgg aaatcaaatt gtatcgtcaa tctcttgttt attacgcaga
     2941 tcaagagtac gagaaagcca ccgatttatt gaaatttcta attgctcgaa cagagtatac
     3001 tcgctttgaa tatattgaac gtttagcaaa catctatcat attatgaatg aacctgtcaa
     3061 agaatatcag ttattggaca ccgtcttgtc agtagcagaa ctgattgccc ttcctgctgg
     3121 attagaaaag aaactagtac gccgcctttt acgagtaaag caacaactat ccgatcaaga
     3181 aaaataactt gcgttttttt aggaaagtgc tatgattatt ttgttgaata aatgggatgt
     3241 agccaaactg gtaaggcact tgactctgaa tcaagcaatt gtaggttcga accctgccat
     3301 cccaatttga tgtgcttgct gaaaaaatga accccctgta aaagaacaat ctttttgcag
     3361 ggggattatt gtattatttt tactacttgt atttagttta cataattaaa ttatgaataa
     3421 taaaatcggg cgtattctta atacaaaaac tgatttaccc aataattgct aatgctttct
     3481 ccggaacttt ttctctagga acttcctcat acgtttctat gtgtgctcct ttattgctaa
     3541 ttttcaaata atgattttct tttaacacag aaagaccatt aaattcaacc gttcgttctt
     3601 tcccattggc atcagcagct ttctgcacat accttgctgt accatattca tttacttcct
     3661 ctggtttttg tgtctttaca tagacctctc ctttttcaac taatggattt agctgatcaa
     3721 gtataccagg gatttctcca taacttcctt cagtataata tttcagtcca aatagcaaaa
     3781 taccactaat cgccgccagt ccaatcaaat atttaatcac tttcatcttt ctacctcctt
     3841 gttatatatt cagtatatgg gtcagttaaa gaaattccat ccgtcccaag ttggagattt
     3901 ttctcctact ttagaagcga gaatcgagtg tttttttagt aaaaatgatc gttatttgat
     3961 attattgacg tgtggataaa tcagtttttc tcgttttgtc catgtaaaaa atcatgttga
     4021 tagcaaagga tgtgttcaaa atgaatcaag aagaattatt tcaaaaagtc aaggaaatga
     4081 ttaagaacgg caatttcgat ggcgcaaaac gatttatcga agaacataaa gaacaattag
     4141 gaccttataa agagaaagcc caaaatcttt taaaagatgt aaatatcgac agtgtcaaaa
     4201 acaaatttaa aaatctattc aaataagtaa aactttcact tttgtaaaat caaaggagga
     4261 caatgctgct gcatcgtcct cctttggttc ttttctgaat acttcatact aaaataaacg
     4321 tgtcggatcg taatgataag tcacattgcc gttcagtgag gcttttccca ccacatattt
     4381 acgattctcc aacttcagcc acgcttctcg aaaagccatt aattcttctc tttttacatc
     4441 aatcttttcg atttttccat cgatcaaatc ttgaataagt tgttcatatg gattcatttt
     4501 tttcctccat ctgctgtcaa tcaataagtt gtaaaaatag gttcatatgc ttttttggta
     4561 tctagatctt ctctgacttt gactgtcgta taaataattt tttctactgg gatactataa
     4621 taacttcctt caccattatt gaatcggtga acgtctttct ttgctaaaat accatttatt
     4681 ttattcgtca gtacacgttg ttcgacactg ttacttacaa catagtaaat cacccgaata
     4741 ttattgatca aatgcaaatc gatttctaac actacatgat ccatgaaaat cccactcctt
     4801 ctttcacttt gattatagca agtttctcct ctaatttcaa aatcgataca gattttaccg
     4861 gataaatcca caaaactgct tgcgccattt cgcacaaagt gttattttgt atcatggact
     4921 ttatttatcg gaggcaatag tatgatcgcg atcggtcaat ttgtttttta tattcctttt
     4981 tttattatga taagcattct tttctattac ataaaatgga caaaaaagaa gttttcagtt
     5041 ttactggcct ctttacctgc tgtatatttt acttatcaaa ttttttcttt taggcattgg
     5101 gaaacaacgt ctgtattaat aacacacatc atcgaattga cgctttcagt aatttttctt
     5161 attatttgga tctatttttt atataagaat cagaattgaa tccctcgcat gtttaaaaag
     5221 aaaacgatta aagatgcagc aaatttctgc cgatctttaa tcgttttttc tattctgtta
     5281 taccaaaaaa atgtttgaat cggtgaagga tcgttccttg ttttaattct ataaattctt
     5341 gtgcgatcgg gtacttttct tccaaatttt ctttgttctt ttcgatcagg cttttcgctt
     5401 cttcatattt tttatctgca catagtgctt ttacttcctg taatactttt cctgcttcca
     5461 tttgcctcac cctcttgcca ttttattgct ttaagtttta tttaagttat acgcattatt
     5521 ctagtttcat cccaaataac cttttttcat ttttactcct cgaactaatc agttttctaa
     5581 aggctgattg gttcgtcttt tttatttttc ctccatttca ttctgctctt ctgttaattt
     5641 atctaatgct ttgattgatg ctgcggctct tgcataatca tagctgtata attccataaa
     5701 tttatcaggc agaacaaacc acgctggatg ataaccgtta gctgtgacag ggaatccttt
     5761 atcttgttgg ttatctccat cccatctcag agcaacacct ttttttcctt tccactttac
     5821 taaagcgatc gagtatccct tctttgtttg tgtgccgtct tcaataattg ctaaaatttc
     5881 tattttttct tttggcgcac ggacttcttg aggacgaacc attttctatt cctcccttat
     5941 ctcattactt cacgaccaac acactgcaag gggcatgtga tacgacataa gaagcggtcg
     6001 atccaactaa tgcttgttgt atagcacctt ttcctgtcgc tcccataacg atcaaatcaa
     6061 tattttcttg tccagggatg acattggcaa tcaaatttct tggatctcca gtttcaacta
     6121 ttgcgtggat ctcagcaatg ccgtactctt tggcatcatt gatttttttc agcatggcta
     6181 cttctgttct ttgtttttca tctgcataga tttttgaaaa ggcataggca ctatgggtca
     6241 gctcaacttt gttgatgatt gacacgataa ataagtgggc ttcattgtcc tttgctaagt
     6301 caagggcttc agcgaacgct ttgatcgatt gtcttgatcc gtcaactgct accatgatgt
     6361 tctggtatct tttactcatt ggaaccgcct cctttttctt ttattgtaac atctcaagca
     6421 aaaatacaaa aaatatacgc aatatgtaaa taaaatgatg aacaaaagca atttctttca
     6481 agttatttat acatttcttt tctcatcaga acacctgttc taatcccatt atttctgcat
     6541 tatgtgaaat tcctcatcat ttttcacatc gagcgaacat cattctgtta caaaaaacgt
     6601 ggatttgtca taaattgtca tgcttctttt acctatgaaa tattttcacg tgttaaagta
     6661 ttcctcgttg aacacaacaa ataaaacttt ttaacgaata aaggagattt ttcatgacat
     6721 cacttaaaac tttacttttt ggaacaactt tggctgccgg cgctgcattc ttcatgggaa
     6781 cgactgctca tgcggacgaa gcttataccg ttcaatcagg tgatactcta tcaacgattt
     6841 ctcaaaaata cgttggtgat aactccttga tcaatgccat tgcagaatca aattcaattt
     6901 ctgatattaa cctgatctac tcaggacaac aattaacaat cccaacagaa ggctctgcac
     6961 aagcagctgc tgaacctcaa gcagccgttc aagaagcacc agtccaagct gaacctgttc
     7021 aagctgaaca acctgttgta caagaaacgg ttcaaacgga aacacaagca gcgcctgttg
     7081 ctgaaacaca acctgctcct gctgtaacag aaactgctgc tactccagca agtacaagct
     7141 ctgcaaaaga atggatcgca caaaaagaat caagcggttc ttacacagca acgaacggac
     7201 gttacatcgg ccgttaccaa ttagattctt cttacttaaa tggtgactac tctgctgcaa
     7261 accaagaaag agtagcagaa caatatgtta cttcacgtta tggttcttgg gaagcggcga
     7321 aagcattctg ggaagcaaac ggttggtact aagattcatt atttgaacgt ttaaacacat
     7381 tcaaaatata gtaagaggtc atgcgattta ttctttcgca tgacctcttt ttgagtttaa
     7441 gtttagtctt tattccttaa ctatcgtttg ccagtatttc ttgtatcaga atctataaat
     7501 ctactatttg aatcaatcta ttaggtttta aactgtccca ccttcacaca aatttttttt
     7561 gatttatttg aatctggttc cgttgtttaa aatttttcct atattctttt agataatatg
     7621 ttattttctt tctcatcgct ccaggtgtac cttctcttta taaaaaaaca aattatgcgg
     7681 ctaagaagac tcgaacttcc acgaggtcac cctcactagc gcctgaagct agcgcgtctg
     7741 ccaattccgc catagccgca tcacatcttt tagtttagga ttacatgtgt tttttgtcaa
     7801 gaacatattt ttgttctttt tcatttctgt acgccctccc ttatgccatc tacacaaata
     7861 attcatccga gcaactccat catctattga gggttggtga aaaaatctgt tttatccata
     7921 tgatcgattt caatgttaaa cgaacgactc tgggattagt aactatttta tttttttctt
     7981 catttctttt ttcctctttt tctcgattca gctatcttct ttcatttgga tattgattat
     8041 ttcttctgtt gattactatc cttctggaat tctagacgct gaagtaattc acgttttctt
     8101 tcttctagag atcataagta acgaatgaac gaacttcaaa taattttttt attgtaattc
     8161 atatttgtat tgttcgatga ttcttaatac gatatgacac aataaataaa aataggcatg
     8221 gctatttaca acaatagtgc tatagtagaa aacaaatgaa taaggttact taaagaggtg
     8281 aatacgatga aactgatcaa actatgggaa gcagatttaa agaaagctta tcagctacaa
     8341 actagctttg gaccaaatga gagtggattt atcaacaatg cttatggcta tacatttgct
     8401 gaatttttag attatgtaaa aaaatgcgaa aaaacgtctt taggaatcga tcttcctgag
     8461 agttacgttc ccgcaaccgt ctttatttta gaaagtgatg aacatgagta tgtaggaatt
     8521 tttaatttgc gccataaact gaacgaaaca ttaagaaacg gcgctggcca tattggttac
     8581 ggcatctccc ctaattatcg taaaaaagga tatgccacac aaggattagc attagtacta
     8641 caagaggcaa aaaaaatcgg cattgatgag gcacttttgt ctgtaaataa aaaaaatctt
     8701 ggcagtctta aagcacaaca aaaaaatggg gctgcgattt atcgtgaaga tgagaaagaa
     8761 tactatacga aaatcaaact gatgtaaaaa agaaggattc caaaaggaaa gggatttgaa
     8821 tgtcctttat caatagaaca catttattta attaacttat aatatagcag acaaaatttt
     8881 ataaacaaag aaacctttta tctcttttct tctagaaagg ataaaaggct tttttgtttt
     8941 tcctatttgc ttctcatagc tagtcagcca agatttctat cgtatcccca acgcaatccg
     9001 tgcataacgg ctcatcttat ctgtcgtcca tgctggatac cagactagtt tgacttctgc
     9061 tttcgttact tctggcactt cttttaatgc gtcatggata gattctgtta aaatatctgc
     9121 taatggacag cccatagttg ttagagtcat cttaatgact gtttctccat tttcaggatt
     9181 gaattctata tcataaatca agcctaaatt gacgatgtcg attcctaatt caggatcaat
     9241 caccatttcc aaggcagcaa gaatacgttc tttgattagt tctatttctt gtccagaccg
     9301 ttcttggttt gcttcgctca tcgtacgaca ctccttaaca cgatttttat ttatcatact
     9361 ctttttttag ctgcttgtaa atgagaattt agaagtaaga ttctcgttta aatttataaa
     9421 aatgttctta ttagatgtaa tagattaaat ttattctaaa ataaacctga ttcctcattt
     9481 tttttgtgaa cgatttttta tttctggcaa tacgccagtt gttcgataca ttttttgttc
     9541 gttttcattt gcaaaccggc caaatactga atgagttggt aaaatattga taaatgcatc
     9601 gtcgtcgatt tcatgaacga tctgatcaag atcatacatt tcgtagcggg tgatgaccat
     9661 catgatcatg ttcccttgtt tttttgaata gccaccgaca gacggcagta atgtcattcc
     9721 acgtaccatc cgttttgcta gttcttctgt tacctcttct ggcttgacgg taacgatcat
     9781 tgctgttact ttttgatgac tcgtgtggat ggcatcaatt acttgagtca ttgcataaat
     9841 ggaaataatc gtatacaagg cactttccca gttaaagaag aagcctgcga tgactacaat
     9901 gataccattt agaatcagca tgtagtttcc gactgttttt ccagttgttt gtgaaagtac
     9961 aagagagatg atatccatcc caccagttgt aaagcccatt tttaaagata atcctacgcc
    10021 aagacctaat aaaacaccgc ctactagggc attcatcaaa atattatcag tgatctgtcc
    10081 gactggaagc agcatcgtca tcaccgatac acatacaaca ttcacaaaac taaggatcgt
    10141 tgctcgtttt cctactttga gaaaccctaa tacaaacaca gggatattca aaagaaaaat
    10201 aaatgttcct gtgtctatct tgatatgaaa ccattcatat aaaagcgagg aaatgatttg
    10261 agcgatccca ttcatccctg cagaaaagac attggctgga atcaaaaaat aatttaaagc
    10321 gaccgcagcg gtcaatccgg ttatcaaaat aaccactatt ttctttaatc tttcattttt
    10381 atgatacgca gtcataattt tactcacaag cacctctccc ctactttggt attccaaatg
    10441 taagaataac agtttatcta ttaaaataaa atacttcgaa ctcaaatgag aagaatttta
    10501 gcaaacaggt aagaatctgc cctaatccta tgataagata gatgtatggt aaatatttat
    10561 atgtatatca ggagggttcc tatggcaaaa gatgctagct tcgacattgt atcagaagtc
    10621 aatacagaag aagtaaaaaa tgcgattcag caaacaacaa aagaattgtc caatcgcttt
    10681 gacttcaagg attctacagt agaaatcaaa ttagagaaca gcaatctagt tgtattgagc
    10741 gatgatgatt tcaaaatcga acagatcaaa gatgtattat ttggtaagct gatcaaaaga
    10801 aatgtgccaa tcaaaaatat ccatttttcc gaatcagaac acgctctagg tggcaaagct
    10861 cgacaaactg ccgatttggt caacggaatc gatcgtgaaa acgcgaagaa aatcactaca
    10921 gctatcaaaa atgaaaaact gaaagtaaaa gcacaaatcc aagaagatca gattagagtg
    10981 acaggtaaga accgtgatga tctacaagct gtgattgctt tattacgtaa acttgactta
    11041 ccaatcgagt tgcaattcac taactatcgt taatcaaaaa acgaggctga cgtctgtcat
    11101 tcctcgtttt tttgatttac tgcttctttt gataaccaaa aacgctgtat tccttcttcc
    11161 aattccccaa cacttcgtac ttctcttgct gtttgccaat gttgtgggaa taaacgtcga
    11221 taagaaaaac tagaaaatcg ttgatctgcc aatacaacga ttccttgatc cgccatgcca
    11281 cgaatcactc gaccaccagc ttgcaagact ttgttcattc caggaagctg ataagcgtaa
    11341 gcaaatccaa ggtgttcctt ctcatcataa tatgatttga tcagttcctg ctcatgattc
    11401 atctgcggca aacctacacc tacgatcata ctgccgatca atcggtcccc tctcaaatcg
    11461 attccttctg aaaaaatccc gccaagtacg caaaatccaa tcaatgtttc agcgggattt
    11521 gtttgaaatt ttgttaaaaa tcgttcgcgt tcttcctcat tcatgtccgt ttcttggatt
    11581 agcacattgc tgtcgggata ttgctgtctg aatgcttcca ccgcaagatc taaatattga
    11641 taagaaggaa agaaaacgaa ataattcccg acctttccat cagcaaaagc atgaatcgtc
    11701 tctactaatt ttgccagact gtgttccctt tttcgataag tcgtctctag gtaatttgcg
    11761 atgaaaactt gctggtttcc ttctgggaaa ggactcggca gtttgtaagc cagacttttt
    11821 tgtcccccca gtgtttcctg ataataagag agtggtgaaa aactagcaga aaaaagaata
    11881 ctgctttttc ctttatccaa cgactgctca agaaacaagc ttggatcaat acaaaactct
    11941 tttactatca gatcgcgata cgttttttcg actgttgttt cgtagtgatc atcataaaat
    12001 tcactgacct tcaaaaaatg aagcaaatca aaataataag gaagcaattg ttcttgtgat
    12061 gggtgctcag gaaattctgc cagccattct ttaatttttt cgcttaattg ataacctgcc
    12121 tttactaaag attcagccgg tgctttttga tgatgataat cccaatgatc ttcttttgct
    12181 atttctcgga tcgaatcaaa ttcttttaat agtttgttga aacggcgatg aagttttttg
    12241 aattcctttg gtatagcttc cttacttcgt ttcgtttttt cataagatag ttctgccgaa
    12301 tacatctctc gggaacgatt caccaaatta tgtgcttcgt caatcaaaaa cagatagtcc
    12361 tcgttttttt catcttcaaa aaaacgtctt aaataaacag ttggatcaaa taaataattg
    12421 taatccccaa tgattacgtc acaccagata ctgacatcta atgatagttc gaaaggacaa
    12481 agtgtatgct ttcttgcata agtttcgatg acttcacgag tgatctgatt ctcatgattc
    12541 aaaagatccc ataaaccttc attaatccga ttgtaataac cattggcata tgggcactga
    12601 tctggattac aggtcgtctc atctaaaaag cagatcttgt ctttcgccgt caaagtgaca
    12661 ctttttactt cagctcctgt atcttttaat gctgtcatag catcttcagc tacttgtcta
    12721 gttattgttt tagccgttag ataaaagatc ttttctccct cttcttcccc tacagctttc
    12781 aaagcgggga ataacgtaga gattgttttt cctgttccgg taggtgcttc tacaaacaat
    12841 ttctgtttcg tacggatcgt tttataagca gctaccgcta gttcacgctg acctttccta
    12901 tactcatcat gggggaattt caaagccatc aaagaggcat tgcgtacagt gcgccagttt
    12961 tcttgaaaaa caagccattc tttataatct tcagtcagct ttttaaaaaa agtttctaac
    13021 tgctctcttg tctgatgttc aacctttctc gtgatcactt cttctgccgt ctggaagtaa
    13081 gtcaattgca gatttatttc ttgtaaatca tgttcttgac tatagatata ggcatacacc
    13141 cttgcttgat gaaagaaaag ttccacttgt tctggttcta aatcttcaaa tcgtggttcg
    13201 gaggttttga tttcatctat ataataaata ccgtctcttt tgaaaatacc gtctgcacgg
    13261 ccttcaacga tcaggtaata ttcatctaat tgaactgttg tttttaaaaa gacttctttt
    13321 tcatagtttt ctcccgcatc tttttggagc ttgcggtgga ttctcgctcc ttctaatgca
    13381 gtatggttac tttttttacg attgtcgata cttccacgtc gcaaaataaa ttctactaag
    13441 tgccgcacag atatttttga ctgtttgatc atttccacca cctctttctc tctaagatta
    13501 cctgaaaaaa cctagtttag caaaaagagc tctctttcca aacataaaaa aacaccctgc
    13561 tgattaggca gggatatagg atatagaatt tggatttttg gatgaatcta atgtaacaag
    13621 gacatattaa aatgctatga aaacttactt tccttctctt tagaatatcc aaatactttt
    13681 catatagtct gataattcag ataagtaaag agaaataagt acctgttttt gtttcctagc
    13741 aatgaatgcg ttatactaat ggatgaaaga agttttggct atcaaggagg aatgcacatg
    13801 catatcgaaa gattaaaaac aggaatgatt gaagaaaact gttatttggt ctacaacgaa
    13861 gaagcacttt tgatcattga ccctggagaa gatgcggaac gaatcaaaca agaaatcaaa
    13921 aaaacaaacc aacagccggt agcgattttg cttacccata ctcactacga tcatatcggt
    13981 gcagtggaac ctttacgcgc gtattatcaa atcccagttt atgtcagccc actagagcaa
    14041 aaatggctag gtgatccgat tttgaatctt tctggactcg gtagacatga tgatattgct
    14101 gatgttattg tctcgcctgc agaatatgaa tttgaaatga aaacttatac tttaggaaac
    14161 atgtcttttc gcgtcgtccc taccccaggg cattctatag gaagcgtcag ctttattttt
    14221 gatgattttg tcgtttctgg cgacgccctg tttagaggaa gtatcggtag aaccgatctg
    14281 tatacaggga atttagaaca attacttcat agtattcgga cccagctgtt tgtattacct
    14341 gatgaatttg cagtctatcc aggacatggc gatgcaacga cgattgaaca ggaaaaaagg
    14401 acaaatcctt ttttcaatcg ttaaaacgac ttaggaggag aataaaatga cagaaactac
    14461 actttatatc gtacgacacg gaaaaacgat gttcaataca attgaacgag tacaaggctg
    14521 gtgtgataca cccctcacaa agcaaggaca agaaggcatt cattatttag gtcgcgggtt
    14581 aaaaaatgtc gacttttcat ttgcttattc tagtgacagt ggtcgtgcaa tcgaaactgc
    14641 ccgtattatt ctaggagaac attctaaggg aaaagaaatt ccttatttta ttgaaaaaag
    14701 gattagagaa tggtgtttcg ggtctcttga aggtggatat gacatggaaa tgtggggtgt
    14761 gatccctcgt gtattgaatt ttaaaacata tgatgaaatg tttaccacag aagtttcttt
    14821 tgaacaaatt gctaatgctg tatacaactt agataccgct gggtgggcgg aaacttatga
    14881 aacattgcga gaccgtgtat ggacaggatt tgaagatatc gcccatcact gcgaaaaaaa
    14941 tggtggtgga aatgttttag tcgtatctca tggtttgacg atcgcctttt tgcttaatct
    15001 gatcgatcct aaacagcctg ttcgtgctgg gcttttaaat ggcagcgtaa caaaggtcac
    15061 ttatgcaaac ggtcgatttt ctatccacgg catcaatgat acgtcttata tcgataacgg
    15121 gaaaacacat agtcgcgact cgtttctata atggggatat tttaattttt accagagaat
    15181 cataaaaggc aaagtctgaa cgagggcttt gtcttctttt ttctcttcgt gtacttatct
    15241 gctttatttc tcaagttttt cttaatatta aaaaacagat tgggtttctc aaaaaataac
    15301 ggtatactat aagatgtatc ttaattggga aggaaggaat ttatgttttt tgcaaatatt
    15361 atcaaagcag tgattctcgg tattattgaa ggaataacgg aatggctgcc catcagtagt
    15421 actggtcatt tgatcctagc agatgaattc atcaaactgg atgccagttc acaatttatg
    15481 tcgatgttca atgtcgttat ccaattaggg gcgatcttgg ctgtcgtcgt tctttatttt
    15541 cacaagttaa acccattttc tccaagtaaa tcaacggtag aaaaaaaaga tacgtggaca
    15601 ctttggttta aagtgatcgt tgcttgcata ccagccatca tcattggttt gcctttggat
    15661 gattggttag aagcaaattt ccacaagttc ttaccagttg ccattgtttt gatcgtttac
    15721 ggtattgcct ttattattgt cgaaaaacgc aacaaaaaca aaacacctcg ttggacttct
    15781 ctgaatgact ttacgtacca agcagctctt cttgttgggt tattccaagt cttggcactt
    15841 gttcccggaa catcccgttc tggcgcaacg atcctaggat ctatcttgat tggtgcttcg
    15901 cgttttgtcg ctacagagtt ttcatttttc ttagggattc ctgtcatgtt tggcgcaagc
    15961 ggcttgaaga tcgtcaaata tttagctgaa gggaattcat tcaaaatgga agaaactgtt
    16021 gttcttcttg taggaacatt cgtatctttt gtggtttccg tcttagctat caaattctta
    16081 ctaagctatt tgaagaaaaa cgactttacc gcttttggct ggtatcgtat tatccttggt
    16141 atcattttga ttggttattg gtttattgca atgtagaaaa aaccagaaca cttctctt
//
LOCUS       DAJSBN010000060        16184 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJSBN010000060 DAJSBN010000000
VERSION     DAJSBN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.060"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            8..1282
                     /locus_tag="ONT86_002275"
     CDS             8..1282
                     /locus_tag="ONT86_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2353034.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(1665..2393)
                     /locus_tag="ONT86_002276"
     CDS             complement(1665..2393)
                     /locus_tag="ONT86_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR2353035.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(2371..3945)
                     /locus_tag="ONT86_002277"
     CDS             complement(2371..3945)
                     /locus_tag="ONT86_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2353036.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            4174..5112
                     /locus_tag="ONT86_002278"
     CDS             4174..5112
                     /locus_tag="ONT86_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2353037.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            5124..6035
                     /locus_tag="ONT86_002279"
     CDS             5124..6035
                     /locus_tag="ONT86_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2353038.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            6059..7531
                     /locus_tag="ONT86_002280"
     CDS             6059..7531
                     /locus_tag="ONT86_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2353039.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            7582..8193
                     /locus_tag="ONT86_002281"
     CDS             7582..8193
                     /locus_tag="ONT86_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2353040.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            8223..10136
                     /locus_tag="ONT86_002282"
     CDS             8223..10136
                     /locus_tag="ONT86_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HCR2353041.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            10264..13245
                     /locus_tag="ONT86_002283"
     CDS             10264..13245
                     /locus_tag="ONT86_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HCR2353042.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            13242..14903
                     /locus_tag="ONT86_002284"
     CDS             13242..14903
                     /locus_tag="ONT86_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HCR2353043.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            14943..15875
                     /locus_tag="ONT86_002285"
     CDS             14943..15875
                     /locus_tag="ONT86_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HCR2353044.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         5250 a         2618 c         3238 g         5078 t
ORIGIN      
        1 tctctcaatg gataatcata ctagaaaatt actcggatta acagataaac atttattttt
       61 tgatgaagaa tggctgattg aaaaagaata taaaggcgtt caagcgcagt ttattaaagg
      121 aaaattggat gactctcctt cctattgtga acactgtcga tcggttcgta ttatccgcaa
      181 cggttcctat acgacacgca ctcagatatt gaaagtcaaa gaaaagttaa ctattttaga
      241 attaaaacgt acccgttttc tctgccatga ttgtggaagg accttttctg cgaaaacgga
      301 cttagttgat gaacatcatc aactaacaaa ggaactgaag caggcaattc tcatggaact
      361 gtacgaaaat caatcacgta aactaatcgc taagaagtac ttcgtctcgg atgggacggt
      421 gacacggatc ttgcgtgaag caacgaagca ttaccagtca cgtatgaact tccttccgac
      481 ggtgttgtgt atggatgagt tcaaatcaat gaagtctgtt tctggttcca tgagttttat
      541 ttgtgttgat gggacaaccc atcagctatt tacgatcctt gaagatcgca gactttacaa
      601 actaactcaa tacttcctgc actttcctag aaaagcccgt ttaaaggtca agtacctagt
      661 gatggacatg aatgccagct attgtcagtt gcttaaaaca gtctttccaa atgcggagat
      721 cgtaactgac cgctttcata ttgtccaaca catcaatcgc agcttcaatc aattacgtgt
      781 ccagatcatg aatcgttttc gaacctcaca ctctgaaaat cagaagaagt accgtcggct
      841 caagcgatat tggaaactct tactcaaaga ttccacgaca tgggagccac tcaaacggca
      901 ttatcatcga ctgtttaaac gaccaattag tcaaacagaa atcgttgatg agctgttaag
      961 ctacaacgaa gaattgcgaa ctgcgtatca tttttgtcaa ttattacgct attatttcgt
     1021 taaacgaggt acggaaggct ttcaagaaac ccttaaaaca atttcatcta cgttacctca
     1081 gtcgtttaaa aagaaattca cgatcttcca ttgctaccaa tcgggtattt caaatgcctt
     1141 taaggtgtcc cactcaaacg gcgtcactga ggggctgaat aacaagatca agctgattaa
     1201 acgaattgcg tttggttatc gcaactttta taactttcgg gcacggatct atttacaaca
     1261 aggtctgatc tttgaaaact aaaaaaatat gcggcgcaca gaacgcgccg catatcggac
     1321 taagttttat tcaaatttgt cttcaccaac acgatttgac gaagagccta tttaaataac
     1381 tacatccatt tattctatct tctaaaatag cctttttcat ttcagttaaa tagttcagcg
     1441 ccatttttgc actcgacttg catagtagtt ttttccatat tcctcaacct cagcgggggt
     1501 tgaagacata aggaaaatcc tatgcgttat gctcaactgg atctgattaa atattctaat
     1561 gtatttttct ctaccatcat taaatggtta atagccaaaa atcaccactt ttcaatggct
     1621 ccttgttgta taagtataac tagaagatga acacgtctga gaaattatag aagcttttta
     1681 cggtaattac tggggctgtg tccagtagtt tttttgaaga gaacagaaaa atagctcacg
     1741 tgatctattc ctactttttg actaatttca tcaatagata agaatgtgtc tttcagtaga
     1801 gcacttgctc ttttgattcg aaagtctagc aagtaatcat gaaaggaata acctgtttct
     1861 tttttgaagg cccgtcctaa ataagcggga tggtaatgga attgtgtagc tacatctttg
     1921 agcgtaagct tttcactata attgttttga acgtaatcaa tcacttgttg aattgactca
     1981 ttaaaagaat catcagttag agagttttga ttagctgctg atttacgcat tttttcttct
     2041 tgtactaact taactaaata aggaaaatca atgggtttcg ttatgtaact tttaatagat
     2101 aaatcaatcc ccttttttgc atactgaaaa ttttggtggg cactaatgac aatactagta
     2161 aagtagcgat tggatgtttg ttgcaaagat tcgattaatt cgagtccaga aattttcccc
     2221 atcgtaatat ctgtgataag taaatcaaag cgctggtttt gcaggttttt aaatgcttcg
     2281 tctccgtctg tgtagcagtg atcgataatg atgtcgggga ctttcttcaa aagataatgc
     2341 cttatgcctt ctacaattgt acaatcatcg tcaacaattg caattctcaa catgcatatt
     2401 cccccctttt tgtattttga tagtaatcgt tgttccgacg tgtagttggc tttctataac
     2461 aaaacgtgta tctccaggat agagatgctt tatacgtcga aaagaatttg agatgccgat
     2521 attctcacta taaggaatat catcattaag tttttgttga atatgctcca gcttgtcttg
     2581 gtccattcct ttcccatttt caacgatttt taacacataa tggttggttg tttcagttgc
     2641 atagacagtc aaacgatttt ccttttttga tgaatcgaag ccgtgtttaa tataattttc
     2701 aacaaatggc tgtaaagaaa gttttggtaa cattaattga tcaatatgat caatcagctg
     2761 atagtcaaag ttatgtcgat aacgacgttg atgtagttgc aaggagaggt cgatattaat
     2821 taattcctct ttcaaatcaa ttatgtcggg ctgatatgta ttattgcgat acaacttgct
     2881 caataaatat atactgtcgc tgatttcttc gtcatcattc aatatggcag tcattcgaat
     2941 aaattccaaa ttattaaata gaaaatgagg ctcgatttga tattgaagtg ctgcatactt
     3001 tgcttcttgc ttcaaccaat tattttcata aagtgttttt tgatatgtag cattatctaa
     3061 ataaaactga ttgatatgca tagcgatttc tttcaaatca ccgactttgt tttcgatatt
     3121 gattgacttg tagtctcctg tagatgaatg atttaaggaa gtaatgattt cctgatactg
     3181 ctggatatag ttacgaaaac ttctatttaa taattgatga atagcaagaa taaacaaact
     3241 cattaataag aaaatcatga ttgccagacc taataaggtt tgtgacgaat aatttggatt
     3301 ttcatatact tgtaatgtgt ggttaccgat agctatctgt tgcgtttttt tccatttaga
     3361 aggcgagatg tcttcagata aattgacgga atcaatacca ttagacaaag aataattgta
     3421 atgtgttgga aagagactct tttttagcgc gttgatcgaa acgtctagaa tcaatgtagc
     3481 atcgggcatg tcctcactaa tgaaacttaa tgaacgaata aaagaaattt tttcgttttt
     3541 tgtaggtgcg taggcactta agtatttttc gtaatctaat gagatagata tatttttaac
     3601 ttcatcatct tgcacaagtt tttttaagtt atctactaaa taggtagggg agcgatcctt
     3661 tgacaaaata tcttccatgt attcagcagg tgatgagagg tagtagtcat taaggttttc
     3721 ttggaaatca taattttgat agatctctgt cacgataccg gagagaatac gattatatgt
     3781 ttccatatag tgggtgattt gctgaatgtt ttgcaggttt tgattttttt ctttttcaat
     3841 cacattgata taggaaaaga acaatgtgaa acaggcaagt aaagtaataa aaaaaatcaa
     3901 tatgtatgta aaacgcataa taacttttga taaatcgttt ttcattcaaa gtcctcattt
     3961 tgataatctc taattttatg aatcattata gcacatatta caagtgatgt aaacgctttg
     4021 caaacagggc tacttatgat tgtgaataca gtttccgttt aaaaactatt ctattgtaga
     4081 ggtttaataa actaaaaata atgtataaaa aattaatgta agcgattaac caagtgggta
     4141 taatcaagga aacgctgaga gaggagagac aaaatgaagc taaagaaagg gttattagga
     4201 aatctaaaaa aaaaccgagc attgtttatt atgcttttac caggtgcgtt atggttattt
     4261 atattttgct atataccaat gtttggagtt gtaatcgctt ttaaagattt cagaatttca
     4321 tcaaggggat tctttacaag tttgttcaac agtccttggg ttggctggga aaatttcaaa
     4381 tatctctttg cttcgcaaga tgcttggatc attactcgca atacggttgt ttataacgtt
     4441 gtaggtattt tgctaagtac tttttgtaca gtgtcattag caatcattct gagccaaatg
     4501 gcgaacaaac ggttattgaa aaagatgcag acatttatga tttttcctaa ttttttatca
     4561 tgggtagtca tcagttactt tgtttacgca tttttgagta cggataaagg attaattaat
     4621 attttactaa gcaattttgg tgtggaaccc ataaattggt atagtgaacc gaagtactgg
     4681 ccttttatta ttgtttttac gagtttatgg aaaggcgtag gctatggatc catcatttat
     4741 tacgcgtcaa ttatggggat cgatactagt tattatgaag ctgctttagt tgatggtgcc
     4801 agtaaatggc aacagataag gaatgtagtc ttgccacatt taagatcgat tattgtcatt
     4861 atgttgattt tgtctgttgg tagtattttt cgagctgatt ttggattgtt ttatcaattg
     4921 ccaagaaatt cgggggctct ttatgatgtg acgaatgtat tagatactta tgtctaccgt
     4981 ggattaatga atagcggaga tatcggaatg tctacggcag cgggattata tcagtcatca
     5041 gttggctttg tcttgatttt gattactaat aagatagtca acaagttaga tcctagttca
     5101 gcactatttt aggaggaatc aaaatgatat caaaattaaa acggcagatg tacttttcgc
     5161 tgccagtcga acggtttttc acaatattac taatcttatt tactcttagc tgtatttttc
     5221 cctttatttt cgtaatcgtc atttcattaa cagatgaaaa agcattacaa ctcaacggtt
     5281 acgaattatt gccggctcaa tggagcactg atgcatatca atacttaata caagatggcg
     5341 gacagttact tcggtcatta ggcgttacaa ttatgattac agtgattgga acgttgatta
     5401 ctgtttttat gacgggaacc tatgcttatg ttttatcaag agcctctttc ccttaccgga
     5461 agttctttac tttttatctt ttttttacca tgttatttgc gggagggatg gttccttctt
     5521 acttagtgat gacgaagatg cttggcttaa aaaatacaat ttgggctttg attctacctc
     5581 ttgcttttag cccatacaat gtcattattt taaggacatt ctttaagaag tcgattccag
     5641 aatcgattat cgaatccgct aaaatggatg gctgtagtga gtttcgagtt tttttccaaa
     5701 tcgttttgcc tttagcgatc ccaggggttg caacaatagg tttattttca agcttaggtt
     5761 attggaatga ctggttcaac gcattgctgt atattgacac caacaaattg attccattgc
     5821 aatatttgtt aatgaagatt caaaattcaa tggaattctt agcaaataat aatgacatta
     5881 cattagcgca gcaacaagca attcaaaata gccttcctca agaatcaaca cgaatggcaa
     5941 tggtcgtagt agctacttta ccgattgcaa ttgtttatcc gttcttccaa aaatactttg
     6001 ttcaaggatt gacgatcggt ggagtgaaag aataaaaaag aataggagat gttaaaagat
     6061 gaaaaagaac atgcagaaat atttgattgg tggtgcttct gtactaaccc ttggcctatt
     6121 aggtgcttgt ggtagcagta atggcgaagc aaataatgaa acaggaacca caaaagttac
     6181 ttggtatcag gttggagaag aacccaaaga ttttgatgag gtcatgacaa aagtcaacga
     6241 aaaattaaag gaagaaatca atgtagagtt ggatatgcgg ctgattccgg acggggatta
     6301 tcagcaaaag cttggtgtga tgatcaattc aggagaagaa tatgatattt gtttcgtcaa
     6361 tggcacagat tatgtgaatt atgggaacaa aggtgctttc attagtctca acgatcattt
     6421 ggataaagag ttgaaagatt atgcagctga attgaatgaa ggatttattg aaggtggggc
     6481 aattaatggc gaaacttatg gattgccagt caataacgat attgccacaa cggaatattg
     6541 gacctttaat aaagatttgg tagatcaata ccaattggat gtagagaatg ttgaaggtgt
     6601 agaaacattg gctccagtat tagaaaagtt tgcagaaaaa aatactgata cgaatattac
     6661 gccaatcggt atgacccgta ccagtgtctg tattccttat gacaatatta tcggtggtgg
     6721 tgtaccgcta gcggttgcat ttgaaggcga tacacaaaca gttgttaata cttatgagac
     6781 tccagaaatg atgacttatt tgaaggctat gcatgagttt tataaaaaag gatatatttt
     6841 gaaagatgca gccactgtta cgacagatcc atttcgaatt gatagtaaaa actggttttt
     6901 aggtcgtaac tcaagtggtt atcaaccatt agcacaaaaa aaattagaag ctctagcggg
     6961 acatggatta attttaaaac cagctggaaa agcttttaaa acgactggat ttgcaagagt
     7021 ggcaatgcat gcggtatctg cttcaagtaa acatccggaa gcagccttga aattgttgaa
     7081 ttatgtgaat accaatcaag aagtagcgga tttaatggtt tatggaattg aaggaaaaca
     7141 ctatgatcgc aatgaagatg gttcgattac tttaacaaac tctgatgatt attatcaagg
     7201 cgcttgggca ttggtaaata cttatctagt ttcaaaaact tcaccgatta cagaagaaag
     7261 taaaaaacta gaagaagaaa gtaaaaagtt tcaaaatgaa gcaccgatgt ctcctatttt
     7321 aggtttctct tttgacgctt ctgattttcg aacagaaatt gcaagtgtac aaaatatcgt
     7381 gtcacaatat gaagtacttt taagctcggg aacagttgac cctgaacaat atgtaccaga
     7441 gttgttagaa aaactagaaa aagctggatt ggataaaatt caggcatctg ctcaagaaca
     7501 atacgatgaa tggcgaaaat ctaacaaata attttttact aaatgaaaag aatagactaa
     7561 gcagttagtg gggggattca aatgaacgaa aacaaatgga ccaaaggatt taattgggtt
     7621 tttcaattgt tgttactaaa ctttacttat tttatactaa tttgtttagg atttggaatt
     7681 ttaggttttt tccctgctac gatagctttg attgctggcg ttgtgaatcg aaaaaaatat
     7741 ggcttgcaag agtctgtgaa gttcatgagt aaaatctata agaaaaattt tgttagatat
     7801 aatctatatt gctggtcagc aactttgttg ttggccggca atattactac gatctggctt
     7861 tgttggcagc aaagcgcacc attgttttat ttattaagta cattattatc ggtaattaca
     7921 ctgttcctag tctttgcaat gttgaattgg gtcgtgatca aaagtcttgg gttgtggatg
     7981 atgggagacc gactgattga ctatttgctt ttcacagcaa cgatgttgcc aacggtagtc
     8041 atgcaaggct tggtgcttgg aatttatttc cgaatattga tgattttgcc tgtgattttt
     8101 ctgtttggcg gaacagctgt aacggtaata ttgattagta aattaactga aagagcgtta
     8161 gcaaagttgc cttgtcagat acaatcgcac taattttttc acaaaaagga gtatttcttt
     8221 ctatgaagtt aactgataaa caagtagcta atatagcctt tatccagcaa cttgagattc
     8281 cattgacaca aaaagatgaa gaacaaatag acttgatttt tactgacaca aaggctaatg
     8341 tgcgacaaat caagattact aaaaatgagc agcaacgaat ttgcttgtct tattcacatg
     8401 aagacttgct gtatcgagga ttgtttatct gccttccacg ttttttagct caaggaggag
     8461 agatgccata cattgaagaa acctcgatca ttgagaccag ttattcatta gatgcttctc
     8521 gtaatggcgt gatgaataaa gagactctaa aaaaattcat ttcttactta gcgttgatgg
     8581 ggtacaacac attgtatatt tacacagaag atacctatga agtagaagac cttccatatt
     8641 ttggttacct tcgcggagcg tatacaaaag aagaagtgaa tgaaattgtt gaatttgctt
     8701 ctggttttgg catagaagta gttccatcga tccagaccct tgctcacttg acccaatttt
     8761 tgaagtggta tgctcaacaa gaactgatgg atgatcaaaa tactctactg atcggtgaag
     8821 aaaaggttta tgcggcaatc gaaaagatga ttattgcctg caaagaaatg tatcagacag
     8881 agcgtattca tttgggaatg gatgaagcct atcaagcagg tctgggacga tatttaagcc
     8941 tgtatgggta ttgtgatcgg acaactttaa tgattcaaca tatggaccgt gtgttatcaa
     9001 ttgttgataa atataagatg aaaccaatga tttggagtga tttcatttat aagctattag
     9061 atgaaaccgg taaagatagg tattacaatc ctgattttaa actcaaagca ttagcagcta
     9121 aaggattacc agcaggatta acctatgttc attgggacta tggttcagat gaagtttcct
     9181 cctatgagaa agtaattgat aaccatttga aattttgtga cattgatcat tacgttttag
     9241 ctagtggtgc ccatatctgg gggagactag ccccgaatca tgggaaaaca gaagcaattg
     9301 tttctgcagc tgtaaaagct tctaaaaata aaggtgtacg ttctgtgatg ttaactactt
     9361 ggggagatga agggcaggaa acagagcatt ggcattcctt aattggggca ttaaaattag
     9421 cagattatgt ctattctgaa gaagagtgga atgattttga ggaaatctac gatcgtttat
     9481 taggaacagg tagttatcaa ttaatgtccg aattacgata tttcgatgaa gtggatacca
     9541 ttacaaataa caacttacaa atggcttccg tgagtaaact gctactttgg caagatccgt
     9601 tagttggtat atatgacttt catgtagccg ctcataacaa aattgccact agaacaattg
     9661 gtacttatta tcatgagtta gctcagaaat tagatatgaa taaaaattgg aatccaaaac
     9721 aaccaatttt aacaactatt cgtcaacgat atatactttt agctgaaatt ttagcacaaa
     9781 aggcagaact aggcatctat ttacaacact gctgtctggt agaagacctt gcaggattaa
     9841 tggaattagc gaatcggtgt gaagcactag ccaatcgagt aaatgaattg gcatctattc
     9901 atgaaataat ttggtatcaa acatacaaaa cctatggttg ggaagtatta gaacaacgat
     9961 atgctgcatt agctcatcgt ctgaaaaccg ttgcgcgtcg tattaaaaat catcttcagg
    10021 ggcaagatgc cctagaggag cttttggaag aacgattacc gttttgtcaa tatcaagtgc
    10081 cgcttgaaat ttctggcttt aattatcggc aaagctcggt gtcaggctac aattaacgaa
    10141 ggaatcttcc cgttgaacgg accacttgct catctaatgg gaaagtggtt cgttttctat
    10201 tcataagtag cttaccacta actcttttat taagaagaga aaaaaagtag gaagtgagaa
    10261 aatatgttat cggactatta tcaaaatctg tcagttataa gtgtcaatac agaaccatta
    10321 cgtaattatt atattcctta tgcaagtaaa gaagaagcaa aacaagagtt agaaagggaa
    10381 aaaacgagcc agtttatcga tctcaatggt acatgggatt ttcagtatta tccttctgtc
    10441 caacagttac cggaaaattt ttttcaagag gcctttactg aaagctatga tagaatttct
    10501 gttcctggag tttggcaaac agagggattt gacttacctc agtatatcaa tgtatcttat
    10561 ccaattcctt ttgatccgcc ttatgtacct aatgataccc cttgtggtgt atatcggcgt
    10621 cagtttcaaa ttgagcaaac agatgcacgc ttttacttaa atctagaagg agcagatagt
    10681 aattgttttg tttggataaa tagaaacttt gtaggctatg accaagtgag tcatagtaca
    10741 tctgaatttg atgtgaccaa gtatttacag gttggggtta atgaaattca aattctagtg
    10801 atgaagtggt gtgatggtac ctactttgaa gatcaggata agtggcgaat gagcggtcta
    10861 tttcgtccag tgtatcttct tcaacgacca actatgcatc tgacaagtta tcaactacgg
    10921 acgttgactg atcctaaaaa aggaagcgga acattaatgg tttcttttca acgatttact
    10981 cctattgaag tatcgttacc tatttcttac gaattattcc atcctgatgg acgtctcata
    11041 gcgaaagggg agtgttactc ggaacaacaa ataaatttgg atgttgaaga ttgccttttt
    11101 tggacagcag aaacgcctaa attatataca ttacttttaa catgtaatgg tgaatatttt
    11161 aaagaaaaag ttggctttcg agcactgacg atcaagaatc gtaagttatt gctgaacgga
    11221 acacctatta aattatatgg agtcaactat catgacgtga atcagcaagg cacgccagtg
    11281 atgacccgag agcagttacg tgcagatttg ttattaatga aaaaattaaa tgtgaatact
    11341 gtacgaacgg cacattaccc taaatcacca gatttttatg aattggcgga tgagattggt
    11401 ctgtatgtaa tcaatgaagc agatttggag tgtcatggtg tggttaatct atatgatact
    11461 gaagcgaatt atcaactaat tgctaatgat ccgacattcg aaaagccgat tatggaccga
    11521 gtgatccgtt ctattcttcc ttatcaaaat cgaagctcta tcattatgtg gtctatgggg
    11581 aatgaatctg gttatggagt aaattttgaa aaagctcaag ctttagcacg gaaattggat
    11641 gattcacggt tgattcataa tgaacgctcg attgaaccag ttattgacca agtgaatgat
    11701 tttagtaact tggatgttat tagtcaaatg tatcccccac tggaagatgt tcatcgtcat
    11761 tgtcaaagaa gtgatactga aaaacctttt tttatgtgtg aatatagcca cgctatgggc
    11821 aacggtcctg gagatcttaa aacgtacttc gatttgattc aacagtatga ttcctttctt
    11881 ggcggttgtg tctgggagtg gactgatcat gcaatcaata gaggaacttt agaaacgcca
    11941 tcttatttat acggtggtga ttttggtgaa aaatatcacg acggcaattt ttgcgtagat
    12001 ggtttattga ccccagatcg gcaattaaca gtaggtgcat tggagtaccg taatattcat
    12061 tcgcctattc aactgattgc tgtagatgag gagctgcaag aagtaacctt gagcaatata
    12121 actgctttta cagccggttc agcatttaac ctttctcttg aatggtatat agatggaaaa
    12181 aaaacagttt atgaagtaat gaatcctttc atacttgagc ctggcaaaag ttgttcattg
    12241 cccatcacag tacctacaac gattcatggt gctcatacgg ttgctgtttc gctatctgtt
    12301 tataaaaatg atgagcttta tggacagcaa cagctctttc ttaaaaaaga aaaaatgaga
    12361 ttgccaagaa ctgctcatca taatgatgca ccacaaattt cagtaaaagg cacagagctg
    12421 ataatcaaag gagaatcctt tatttatata tatcaattag caaaagctgc gttcacttct
    12481 gtaaagatca atcaaaagga acttttggaa cgtcctactc aattttcaat ttggcgtgca
    12541 ccaacggata atgatcgtaa cttagtaaac aaatggaaag cagctggata tgctgaaatg
    12601 tctttagaaa tcagaaaaaa agactggaaa ttcgtagatg atcatctcct gatcgaaacg
    12661 tcttatcgag gagttattcc aggattacag ccaattttta caatgcacat acagttgaaa
    12721 ataaatggtg caggcgtaat caaccaaaaa gtatcagtgg agaaaaatcc gatttttcca
    12781 gatttccctc gctttgggat ggactggccg ttgaatgata ctttttcgaa ggtttcttat
    12841 tgtggctatg ggccgtatga aagttatcct gataagcact atgctgcgat gtttggtatt
    12901 tatgagatgg agattgagga atttgatcag ccatatatca aaccacaaga atatggtaac
    12961 cgctggcaat gtgattgggt atcaatcaaa aatcatcaag gatctaccat gacggtttat
    13021 ggaaactcta atttttctgc tctgccttat agtcaagctc aattaaccga aaagtcacac
    13081 cattatgaat taataaaaga aaatagaact tggttcaatt taagttataa acaaaatggt
    13141 atcggctcac acagttgcgg tccttattta gaagatcgtt tccgttttat ggaagatact
    13201 tttgactgga atatagtgat tactttcaaa ggagaaaata gatgaaagaa acaaagatca
    13261 atcaagtcaa aaaaaaatta tctcgttatc aagaagttgt ttatcgaaac cattgcttaa
    13321 aatggtcaaa aaataagctg aatgttcgtt ttaatggatt attttatcgt ttcgaacagt
    13381 tagaagaagt ctggaaaccg gtaacgaatt tccaatcatg tcctgtacag ctcataaatg
    13441 atccaaaaga aacatacgat ttatgtataa cacaccttta taaccagcat ttgaaaaatg
    13501 acggatttta tttaaaggtt actaatgaca atcaacttac gattgaatca gcaaaccttc
    13561 gagggtttca atatgctatg gaagccttat tagagagttt tttttcggta ggagatcaat
    13621 tattattacc ggaattgatt ctgaaacatg aaccatcagt gaaaattcga ggaatcattg
    13681 aagggtttta tggtatcccc tggacacatg aaatgcgcct ctctcttttt tcatatatga
    13741 aagataacca attgaatact tttatgtatg cccctaaaga tgatgaatta ttacgtaaaa
    13801 aatggcgtga actctacgat gcgcaagaat tagacaaatt taaggagctt ttatctgctg
    13861 ctgaggctag caatatcgat ttttggtact taattagtcc tggaaatgat attgatatta
    13921 cgtgtgaaga agacattcaa gttcttctga aaaagttaga acaactgatt gagcttggcg
    13981 tatttcaatt tggtctatta atggatgata ttgactatca attaaaaggt gctgctaaac
    14041 gccgttttcg agagccagca ttcgctcatg cctatttagt gaatcgagta gaggaatatc
    14101 ttagcactcg gttgtccaat catgaattag tcatttgtcc tacagaatat gataatcgtc
    14161 atggttctcg ctatttagca acgttaagtc aagaaatacc agaacaatta ccgttctttt
    14221 ggactggccc aagcacgtta gcggcgtcaa tttctacaga agagctgcag gaaatggctt
    14281 ctgtctatca acggccgatg ctgatttggg ataatattcc ggtaaatgat tatttagagg
    14341 ataaagaact attgttcatg agtccatatg agaatcgaac accgaatctt tctaaggaga
    14401 gataccaggt tacaggagta gtttcgaatc caatggctca attagaagct tcaaagttta
    14461 cgatcaatag tatggctaat tacttatgga actgtgagcg ttttgatcct ttggaaactt
    14521 ggacgtcagt cgttgaaaaa gtagttggaa gtaaactaca accagcttat cttaccttaa
    14581 ccaatgcttt tccaaatcat tacacaagca gtctgctttc agatgaagaa ttactgctag
    14641 ccaaagaccc agaatgggtc acaaaagaaa tggctgcttt agtccaagca gtaaaatgga
    14701 tccagcaaga acaacataca tctagattga gaacagaatt ggagccttgg ttacaagcaa
    14761 tcacagaaag ttggactttc tggcaagaat ggcaattaaa aaaagatcca aaagaagcag
    14821 aagaatggtt ggctaagaaa aaaacacacc gcataggacg tgatttagta actgcccatt
    14881 tagagcaaat tatcaaaagt tagatatctt aaaaaataaa aagcaagaaa aaggagaaca
    14941 aaatggtcaa gagattaatt agtgcaaact acagtgatgt caagaaaatg acgtcaaaag
    15001 agttgaagca atccatcaaa gcgagtgaag gacgggtgat tgtttgtgaa acggttgcta
    15061 atactcttcc acaatctgga gaaattacga atgcagaagt tgcgtcagca tttggtgcag
    15121 atttgattct tttaaatggt ttcgattgtt atcgtcctgc agtttttgga ctaccaaatg
    15181 tgacgattga gaaggttttt gatctacagc aaacgattga aaacccgatt cctaaattaa
    15241 aagaactagt cggtcgtcct gtgggaatta atttagaacc cattgcccct aaaacaaata
    15301 tgatgagtga gaaagtagaa atcagccaag gaagagcaag ctctgttgaa acgatacaaa
    15361 aagcagaaga actaggagtc gattttattt gctttaccgg taatccaggc actggagtaa
    15421 ccaatttaga aattactaaa tccgtaaaaa tggcaaaaaa gtatttttcg ggattgatta
    15481 ttgctgggaa aatgcatagc gccggctcag aagagccagt tgtgaataaa gaggcgatta
    15541 tttcttatgc ggaagcaggc gcagatattt tactagttcc ttcagtaggg tctatcccag
    15601 gctttttaga gcaagaccta attgaagcgg ttcggttggc aaaaaaatat gatttgttaa
    15661 cgatgtctgc aatcggtact agtcaggaaa gtgcccgacc agaaacattg cggcaattag
    15721 ccattatgaa taaaagttgt ggtgttgatt tacaacatat tggagatgct ggatacgggg
    15781 gattggcacc agcagaaaat attttcgaaa cctcggttgc aattagagga atgcgacata
    15841 ccatcaactg cgttagccgc tcgatcaatc gctaacaaaa ttaaataaaa aatggttaaa
    15901 ttgattattt aagtgggaac atacttgaga tatcagctaa cttctgtcac tttaaaaaag
    15961 ggactgtgac agaagtgaaa atagacaaaa aatatccgaa caagagacta ggatagcaga
    16021 aaaactatcg ttttgcaacg aggaccaact ggaccgtagg agcaattctc ttattgttcg
    16081 gatatttcgc ttgttcaagg acttatgtct gtagtgccct tttttggtag gaaaaatggt
    16141 agggaaccat atcattttta atgaaaatca agcatttcaa aagt
//
LOCUS       DAJSBN010000061        15230 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJSBN010000061 DAJSBN010000000
VERSION     DAJSBN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.062"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            278..787
                     /locus_tag="ONT86_002286"
     CDS             278..787
                     /locus_tag="ONT86_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2353045.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ONT86_002287"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ONT86_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2353046.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ONT86_002288"
     CDS             2322..3302
                     /locus_tag="ONT86_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2353047.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ONT86_002289"
     CDS             3424..4842
                     /locus_tag="ONT86_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HCR2353048.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ONT86_002290"
     CDS             4913..6382
                     /locus_tag="ONT86_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2353049.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ONT86_002291"
     CDS             6392..6859
                     /locus_tag="ONT86_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2353050.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="ONT86_002292"
     CDS             6895..7767
                     /locus_tag="ONT86_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2353051.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="ONT86_002293"
     CDS             7848..10382
                     /locus_tag="ONT86_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2353052.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="ONT86_002294"
     CDS             10538..11311
                     /locus_tag="ONT86_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2353053.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="ONT86_002295"
     CDS             11304..12095
                     /locus_tag="ONT86_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2353054.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="ONT86_002296"
     CDS             12105..12596
                     /locus_tag="ONT86_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2353055.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="ONT86_002297"
     CDS             12614..13024
                     /locus_tag="ONT86_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2353056.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="ONT86_002298"
     CDS             13028..14428
                     /locus_tag="ONT86_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HCR2353057.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="ONT86_002299"
     CDS             14451..14825
                     /locus_tag="ONT86_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2353058.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DAJSBN010000062        14939 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJSBN010000062 DAJSBN010000000
VERSION     DAJSBN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.063"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..562
                     /locus_tag="ONT86_002300"
     CDS             <1..562
                     /locus_tag="ONT86_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR2353059.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="ONT86_002301"
     CDS             762..1076
                     /locus_tag="ONT86_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2353060.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="ONT86_002302"
     CDS             1097..1474
                     /locus_tag="ONT86_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2353061.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="ONT86_002303"
     CDS             1484..2257
                     /locus_tag="ONT86_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353062.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="ONT86_002304"
     CDS             2279..3682
                     /locus_tag="ONT86_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR2353063.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="ONT86_002305"
     CDS             3864..5048
                     /locus_tag="ONT86_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2353064.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="ONT86_002306"
     CDS             5045..5335
                     /locus_tag="ONT86_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353065.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="ONT86_002307"
     CDS             5332..5553
                     /locus_tag="ONT86_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353066.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="ONT86_002308"
     CDS             5595..6374
                     /locus_tag="ONT86_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HCR2353067.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="ONT86_002309"
     CDS             6441..6935
                     /locus_tag="ONT86_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR2353068.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="ONT86_002310"
     CDS             6991..7629
                     /locus_tag="ONT86_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353069.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="ONT86_002311"
     CDS             7701..8096
                     /locus_tag="ONT86_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2353070.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="ONT86_002312"
     CDS             8080..10533
                     /locus_tag="ONT86_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2353071.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="ONT86_002313"
     CDS             10530..12557
                     /locus_tag="ONT86_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353072.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="ONT86_002314"
     CDS             12554..13576
                     /locus_tag="ONT86_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR2353073.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="ONT86_002315"
     CDS             13593..14522
                     /locus_tag="ONT86_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2353074.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="ONT86_002316"
     CDS             14767..14883
                     /locus_tag="ONT86_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR2353075.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         5072 a         2485 c         2954 g         4428 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgc
//
LOCUS       DAJSBN010000063        14569 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJSBN010000063 DAJSBN010000000
VERSION     DAJSBN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.064"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(374..736)
                     /locus_tag="ONT86_002317"
     CDS             complement(374..736)
                     /locus_tag="ONT86_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353076.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(810..1190)
                     /locus_tag="ONT86_002318"
     CDS             complement(810..1190)
                     /locus_tag="ONT86_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCR2353077.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1254..2447
                     /locus_tag="ONT86_002319"
     CDS             1254..2447
                     /locus_tag="ONT86_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2353078.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2524..3108)
                     /locus_tag="ONT86_002320"
     CDS             complement(2524..3108)
                     /locus_tag="ONT86_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR2353079.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3309..4307
                     /locus_tag="ONT86_002321"
     CDS             3309..4307
                     /locus_tag="ONT86_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2353080.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4419..4928
                     /locus_tag="ONT86_002322"
     CDS             4419..4928
                     /locus_tag="ONT86_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR2353081.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4958..5617)
                     /locus_tag="ONT86_002323"
     CDS             complement(4958..5617)
                     /locus_tag="ONT86_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCR2353082.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5619..6383)
                     /locus_tag="ONT86_002324"
     CDS             complement(5619..6383)
                     /locus_tag="ONT86_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCR2353083.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6391..7068)
                     /locus_tag="ONT86_002325"
     CDS             complement(6391..7068)
                     /locus_tag="ONT86_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCR2353084.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7105..8490)
                     /locus_tag="ONT86_002326"
     CDS             complement(7105..8490)
                     /locus_tag="ONT86_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR2353085.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8700..8784)
                     /gene="ffs"
                     /locus_tag="ONT86_002327"
     ncRNA           complement(8700..8784)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ONT86_002327"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8807..9322)
                     /gene="tadA"
                     /locus_tag="ONT86_002328"
     CDS             complement(8807..9322)
                     /gene="tadA"
                     /locus_tag="ONT86_002328"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR2353086.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9429..10286
                     /locus_tag="ONT86_002329"
     CDS             9429..10286
                     /locus_tag="ONT86_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2353087.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10367..10702)
                     /locus_tag="ONT86_002330"
     CDS             complement(10367..10702)
                     /locus_tag="ONT86_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353088.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10769..12139)
                     /locus_tag="ONT86_002331"
     CDS             complement(10769..12139)
                     /locus_tag="ONT86_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCR2353089.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12217..12753)
                     /locus_tag="ONT86_002332"
     CDS             complement(12217..12753)
                     /locus_tag="ONT86_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2353090.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13062..14543
                     /locus_tag="ONT86_002333"
     CDS             13062..14543
                     /locus_tag="ONT86_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2353091.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4485 a         2898 c         2366 g         4820 t
ORIGIN      
        1 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
       61 atgtcgttag taacatgtca ataactattt tttaaatttt ttagaaatgt gatataacat
      121 ccacttcaga atcaacgctg ttaaatttat catgtatttc ttccttggtc aactataaat
      181 taagcattac tttgtgaaat gatttttcga tcaattatta ttattcgtta tacaaccgtt
      241 ttcaacatat tttgaaaata aaatgcacta aaaaactatc tttagcagaa catttcattg
      301 tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa aattcaaact tctttgttcg
      361 cttaatctgt attttagaag aaaggcactc cctctacttc gtaagttcca agaacagagc
      421 gcatttgacc tggttcttcc caaatatatc cttcgcctaa ttcttctctt tgattctttt
      481 gttcagtttc aaagacaaac aacggaacat tttcctcttt aatatgtgta tttgtcaatt
      541 ccatcaaact aatcttgcta acatcgatat gaactatttc ttttaagaaa ttaagtatat
      601 tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc aaattccaac ttgtcatcaa
      661 ttgaatggac caaaaatttt ttagtaccat cctccaaatg caacataatt gtaccagcaa
      721 ctcttttgct tgccaaaatt tccacctcga tcaacttgtt ctatggctta cataaaaatt
      781 ctagcacatt ttcagaaaaa atacactttt tatttcttat tttctttccg ttctagttca
      841 gataatgctt cttctatcca aataggtaac tgctccgcaa tagtggtaaa tccgatctta
      901 cgatttttat tcgtgaaata cctttttctg cgatatggaa cagctggatt tgttttttct
      961 aacgttctta aagataaact gatttttttg gaatattcat caatgtcgat gatctgaact
     1021 gttactggct gtccgacttt taacaatgaa tggatatttt tcgtataacc ggattgtact
     1081 tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag aaacgaatgc gccataaggc
     1141 tgtatccctg tcacttttcc ttcaattatt tgtccaattt tataagtcat ctacttccct
     1201 ctcttcgcat ttatcattat actgtaaata gctcaaaaaa agaagcgacg aaaatgactg
     1261 gaggtcttaa gatgatcgaa tttgataaac agatttcaag aaaagataca gaaagtgtaa
     1321 aatgggacgc aattgctgag acatatcaaa tggatgactt acttcctttg tgggtagcag
     1381 atatggactt cttgtctccc ggtggggtag ctaaagcgtt tagtgaatac atcaaacatg
     1441 gaatctttgg ttatgccact ctgtctgata aactatatca ggctatcatt cattgggaac
     1501 gccaacacca tgctgtggag ttgacgaaag aaaatatcgt ttttacaagt ggtgtgttaa
     1561 ccagtttagc tgcagcagta caaactttta caaaaccagg cgattctatt ttgattcatg
     1621 acccagttta tcctccattt tcctctatta tagaaacaaa tcaacgtcac atcgttcgaa
     1681 gctctttgct agaaaaaaat aatcatttca tcatggactt gtctgacatg gagaaaaaaa
     1741 tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc tcacaatcct ggagggcgtg
     1801 tttggagcaa agaagaattg aaacagttaa gcgaattgtg tttaaaacat caagtgattc
     1861 tgtttagtga cgagatccat caagatttaa ctttattcga tcatacattt acttccatgc
     1921 taacgattga tcctaattta gatgatctac tagttgcttt tacttccgca accaagactt
     1981 tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa aaatccagag atgaaagctg
     2041 attttgaaaa acatttagtg atgaaccaac agcacgaaat caacactttt ggactgattg
     2101 gaacccaagc ggcttatgag acgggtggag aatggttaag ccaactgatt ccttatctag
     2161 aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta tcttccgaaa gtgagaataa
     2221 tgaaccctga aggaacttac ttaatgtggt tagattttac cgcctatatt tcagacgacc
     2281 aagaattgga gaacactctt gttcggaaag gaaaagtcgt attaaatcct ggcatcacgt
     2341 ttggtccttc cggtcatggg catatgcgct tgaatattgc ttgtccagaa gaaactttac
     2401 tggaaggact taatcgaatc aagtatgctt tagagacaat cgattaaatc atttattcaa
     2461 aataaaagga cgagacctta ctaggtttcg tcctttttca taattactcc gtaccatacc
     2521 aaactattct gaaatttcaa ttgtttcaat cactacatca tgtacaggtc ggtcttgtgg
     2581 accacgttgt acatttgcga tttcatctac tgtgtccatc ccttctagta catgtccaaa
     2641 gactgtatgt cggaaatcta gccaaggagt tccgccacct ttatacgctt caatgatttc
     2701 tgatgggaat ccagcacctt ctagttgtcc aagcatatta gctggcacat ttgtgttatt
     2761 tacaataaag aattggcttc cgtttgtgtt tggacctgaa tttgccatag ataaagctcc
     2821 gcgaaggttg aacagttcac gactgaattc atcttcaaaa ctttcgccat aaatactttc
     2881 gccacccatt cctgttccag ttggatcgcc gccttggatc atgaaatcag gaataacgcg
     2941 gtgaaaaatc acgccatcat aataaccttt tttggaaagt tcaatgaaat tttgaaccgt
     3001 cttcggtgct agttcaggga ataattgaac agtaatgtca ccacggtttg ttttgataac
     3061 agcttttggt cctttttcac tttttaaatc tagttgtggg aatgccatat tgtttcctcc
     3121 taatatttgc atacttcata ataataataa cataatacct aacctctcgt aaaaatacta
     3181 gatttgtttt cttttatttt tcatcactag atacattcta taacttaccg tttcgatttc
     3241 gaattcaaac atttttgaga agcattctca attaaattct gctatagtgt gaaaagggag
     3301 gaatttttat ggaaatctat gacattacaa tcatcggtgc aggtcctgta gggatgtttg
     3361 ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat cattgacagt ctacctcaac
     3421 ttggtggtca attagcaact ttgtatcccg aaaaatatat ttacgatatt cctgggtatc
     3481 cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa acaattaact acttttaacc
     3541 atacattcca tttaaaagaa gaagttctct cgcttactcg tgaagacgaa gttatcgaga
     3601 ttactacaaa taaaggcatc cattattcca aagcagtcat tttagcttta ggaaatggtt
     3661 cattccagcc aagaaaattg aatttagata atgctgaatc atttgaaaac cacggattgg
     3721 attatttcgt gaatgatttg atgaagtatg ctggaaaaaa agtcgctatt gctggaggtg
     3781 gtgattctgc tatcgattgg acgttgatgt tagagcctat tgcaagcgaa gtttacttga
     3841 ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt atcacgcttg aaatcttcat
     3901 ctgtcaactt gcttactccc tatctcatcg atggattatc aggcaataat ggggaactga
     3961 cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat tgatttgatg attgattctc
     4021 tgatcgtcaa ttatggattc tcttctaatt tagaacatct ttcttcttgg ggacttgata
     4081 gtaccagaaa tgccattact gtaaaatcag atatgtctac ttctattcca ggcgtttatg
     4141 cagctggtga catctgtacc tatgaaggaa aagtgaaact gattgctact gggctcggtg
     4201 aagcacctac tgcagtgaac aacgctttac actatattaa tccaaaggaa cgaactcagc
     4261 ctggtcacag cacaagctta tacgataaaa atcttcgtcc tagttgattt tttatatctc
     4321 tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt tttttcatgt tccgttaaca
     4381 tgaaatagag aaagaaaaaa taaaggggga gcaataatat ggtcgtcaaa actgagactt
     4441 tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt aactgtagaa gatatcgcac
     4501 aacttgtcat gtttttacaa aaagactata tttctgattt gactttggcg gattgtactg
     4561 aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa cgccattatc actggaatcc
     4621 aattagatat attagcagaa aagaaaaaat tgatgagtcc gttgcaacat attatcgaag
     4681 atgacgaagg cctttacggt atcgatgaga tcctagcctt gtcaatcgtg aatgtttacg
     4741 gtacgatcgg ctttacgaat tatggttata tcgacaaggt gaaacctggt attttaaaag
     4801 aactaaacag ccacgatggc gttcatgtcc ataccttctt agatgatatc gtcggagcga
     4861 tcgccgctgc tgctgccagt cgattagcac acaaagaacc agaaaaaaga agtcgcctta
     4921 ctcaataatt caacaaaaaa agaaagaaaa cgtctattta gacgagttct ttcttttttg
     4981 tttgtttcaa gctatttttt cctaagaaaa gaaaaacagc aaaaaataat tctaataata
     5041 caaaaaataa gataaagcta tgcatgaaaa attcttctgg aagtacattg atgattggat
     5101 cagtaacggg atcgaataac cagtcatcgt tgttgaaaaa cgtttcatga aaaaggataa
     5161 aaaagcgatc aaatcctatc atcatgaaaa aaccgaaaac aactggcagt aacatgccga
     5221 tttgaaacgg acgaatcaac cgccaaagcc gatcattctt ttgcaaatat ttgataaaca
     5281 aaatactcgg gatcaaagtg atgaaaaaca cagcataatc cactaaaaac aaaattttca
     5341 cttcataaaa gtgatgggct ccgctagcag aaactgggaa atctggcaag ctgaggatgg
     5401 ttttgaaggg attattgaga taattcatta ggtgatcaaa atttttcagt aacgtctctt
     5461 gatctaaaga agtataatcc aaaatttgca aatgtccgat atcaaatacg tatagtggtc
     5521 gaaagttgat cgtcacacca actgccagac tgatcagtgt cagaaatagg ctgaacaatc
     5581 cagcatattc catccactgc catctcgaat ttttcatatt agaagctcca ttcatccagt
     5641 gattgtttca cataggtagc tgggataggc aacccaggta catcttcttc ttttgtaaaa
     5701 cctgataaaa cgagcaaagt atctatatta ttgcgtatcc cagcttggat atctgtttca
     5761 tagttatctc caaccatgat tacttcttct ttttgcagcc ctaatacttc aacggcttta
     5821 tccataatga tggcttctgg ttttccgatg tagatcggcg gcgtttgggt tgctgtctct
     5881 acaaaagaga tgactgaccc tgctccagga agaaggccgc gttcagtagg tatattctta
     5941 tctgggttcg ttccaatgaa agtagcacct ttttgaatag caagtgttgc tttaactact
     6001 ttttcatagg tcagataatt atctaagcct accactacat agtcaggagc ctcttcttcc
     6061 catgtaaatc cggcagctaa aatcaagtca atcaatcctg cttctccgat aacatagact
     6121 tttttccctt ttccatctgc tttcataaaa tcgattgttg ccaaagtagc tgtgtagact
     6181 gtctctggag ctacatgaat gtcaaactca tctgctagac gattagctac tgtttctggt
     6241 gacttagtcg tgttattggt aacaaacaag aaaggtaatt ttcttttttg caattcttcc
     6301 acaaatcgtt taccggccgg tatgggttgt gtcccacgat aaatcgttcc gtctaaatca
     6361 attaaatatc ctttatagtc cattcatcca ctactcttcc ctttttctga tcgtaaatgc
     6421 tgtttttgtt ttttctactt gtttaggttg ttcttgtttt tttacttttt cagaggtttt
     6481 tgctgactct gtttttcgat ttttaataac cggttttttg cgattagatg cgactggtcc
     6541 ttttttctca tcgatatacg cacgattatg gttttttttc ttttttcggc gctgagttgt
     6601 cttttctttt ttaccgccga ttctctcgat gacaaaatac gcacaaccaa aattgcaata
     6661 ttcgtaaaga tagtcttcca aggaatcgat tcgctgatct ggatgcgccc gtcgattatc
     6721 cgcttggaaa aatcctttta ggcgtagttg ttcataaccc caatctccta cgatataatc
     6781 atatcgggct agtacatcac tgtaacgttc tccaagcttt tctgcattaa atccttcacg
     6841 ataatcggac acaagccgat aatgtctttc acctatcaaa aaattactgt catctaacag
     6901 tgtcactagc tcgcctttga ctttttctgc tttaggctcc tcaacgattt cttccaaagc
     6961 agctgtcagt tcttcggtga ctgttgtttc ctgttcagct gatgattttt tcttagcaat
     7021 ttctgtttta tgctcaacgt tctgctgttc attttgtttt gcagacatgc gttcaccacc
     7081 tatctttttt attatacctt gtttttattt gattgggtag tgagctttca agtaatctcc
     7141 taccacactt cggataaatt cagggaacaa aaattcattt tttccagcaa tctcaatcgt
     7201 tggaaaaaat ggaacaaaca tcaaatgatc tacggtagta aaagtataat ttttattcag
     7261 atcgattggc ttgttcttcc aaaagacttg atggttaata gaatcatatg tgattccatt
     7321 atagaccatc tgcccaaaaa tcttaccgcg aaatcccatt cccttcatag gatagttacg
     7381 taaaaagtta cgattttttt ctatttctaa aactaagcgg atcagatcat ttccacccaa
     7441 tgtgacattt aacaagtgca tcggatgcgg caacgcagtg tgaagctgat cctgattgac
     7501 ccgccctttt ggaatttcag ttaaaaacag accactgttt aatattgctg cttccgtctt
     7561 tcctcgttct ttcaccgcct caaaggctac ttggataaac gaatgctcat tgaagatatc
     7621 tagactcaga gaaaatggca aatctgctac ttctttttct tttaataatt catgcccacg
     7681 ttctagataa ctatttattt cttgttcatc ttctgtaaaa gctgtcattg tttcggtagg
     7741 gatggctttg gctgaatgct taaggatttt tttctcttca tcgatggtta aatggacttc
     7801 accgacatac tgcccgtact ttccagcggc tgctatttgg acatcattga ttacgtgacc
     7861 ttctctgaat aaatgatggg tgtgcgatcc taaaataaca tcaattgctg gtatttcttt
     7921 tgcaatttgg aaatcatcct gaatcccgag atgactcatc aaaactaaaa catccacttg
     7981 tgaacgtaaa tcttcgacta attctggcaa aatatcataa ggattccgta tgtcccagcc
     8041 attcggatta taagtaagcg gaaatggagc agtaaatgca attagtccga ttttcgtctg
     8101 gtgtttcgtt gtaatgattt tatatggttt cgtccatgcc ggtggctgga gcgtattttt
     8161 atcgaataag ttatccagga tgatatcaaa ctcagcatga tcgtataaat gattcaactc
     8221 ttctttggaa tttcctacgc cttcattgtt tcctatcgtc gctgcatcat aaccaacttc
     8281 attcatcagc aagatatttt cctgtccatt cgttgcttct gacaatggat gccaacgatc
     8341 cacaaaatca cctagatcta ctgcaatcgt tgtcgttttt tctttctttt cgttttctcg
     8401 tttacgttga tccaaaaacc ttctgatcct tggccaattt tctaaatgag aatgcagatc
     8461 attcgtatgt aaaatcacta ttttttccat gtaccctctc gcctctcttt atctttatca
     8521 taccataggc agctttttct tccattgaaa caagctctat ttcagagttt ttcctgcctt
     8581 gaagcaaatt gtataagtac aatattcgat aaatcagtct ggctaaaaaa tcaggaacat
     8641 tcatttttac agtactataa aaataaaaaa aacagacccg aaagtctgtt gtcgtttcat
     8701 tataatcaga cacatggcgg aacctgctta gtgctgcttc cttccagacc tgacacgctt
     8761 cacaagccca ccattgtcct agcctttcgg caaagaatag tataccttat ttttcattcg
     8821 ttgaccagtc tttttttaat ttcttttttc gctggcggag ctttcggaaa aagtcagtca
     8881 aaatcatccc acattcgtct tctaaaatgc caccttctac atatgcttgg tggttaaacc
     8941 gctcgtcctc caaaagattc atcaaggtac cggctgttcc gccttttgga tcatacgcgc
     9001 cgaaataaac ttctgctaca cgagacaaaa tcatcgcgcc gctgcacata ggacaaggct
     9061 ctaaggtaac aaaaagtgca gcatcttcca agcgccagct tcccactttt tcacaggctt
     9121 ccttgattgc aagcatttct gcatgtgtag tagcatcttg cgtggtttct ctaaggttat
     9181 atcctcttcc aatcactttt ccatccaata caaccactgc tccaatcgga acttcatgaa
     9241 gcatttctgc tttctttgct tctgccaatg caaggcgcat ccatttttct ttttctactg
     9301 catcaagatt tgtatttacc aattaccctt ccctgccttt gttttttctt gttttacttt
     9361 agcatatgat aaaaaggctg taactaactt ttttttctat ggtaaaataa atacaagagg
     9421 tgaacaagat gaaaatggat aaattacttg ctctttaccc acaagcacag aaaaaaaact
     9481 ctccagcatc agaagcggat atcctttctt ttgcaattga tgatgctttt atctggatca
     9541 aacaagattc attatctcaa caagaaacca gccttttgaa agcgttattc cctgtaatca
     9601 acgatcaaaa aaaacaccca tggtatcgct atctttttaa agatgcttct tgtgttcaag
     9661 aaaaaagctt tcgtttgatc cagcttcacg tcgaatcaaa aggagaattc ttaaaaaaag
     9721 aatggcaaga tactattttg gaattatttc gctattctgc tgatttcttt ttttatacag
     9781 acacagatgc gctcttagtc gaacaaataa accgtgatta tcttgatgct actgaaatca
     9841 acggtatttt tctatcttta gatgctgatt ttgatacgac aacgtctgta ttcgttggtt
     9901 ctttccattc acctggcacc gacctcgtcc ctttgtttgc tgaagaaaga aaaatttttt
     9961 taacagagca aaacaatctt tatactcaca gccgtacttt ttctatgcaa gatgtggctt
    10021 tacattacta tacaaaagat actgtaaaag atagtaagtt gatcaaaagt tacatagaac
    10081 tgatcaaaca aaatgaaatg gaagtgatca ttcaagaatt atggaatgaa ttaggcaatg
    10141 tcagctctgc tgcaaaaaca ctgtttcttc atcgtaatac tctaaaatat cgaatcgaaa
    10201 aatttcaaga acagtctggt ttcaatctaa aaaaagccaa cgatttactg ttttgctatc
    10261 ttttattgct tcatgaacat aattaaagat agattaaagg ggttgtgaca ataattttgt
    10321 cacaacccct tatttcgaat gaatagtgtt caaaagctag tttctcttac atcatttttt
    10381 caacaaaatg cattagccgg tcttttacat cttcggttga atctttgatt gaatcaccat
    10441 aaacagagat tttctttttt cctgatttga tttttgctaa cattcccatc aaagaatcca
    10501 agtcactatc tgaaagacca tctacgattt ctagtaattt ttcatttcca tcaaatttgt
    10561 catagacgaa tttttttact ttcgtccgag tagtcatatg atggatcttt tccattactt
    10621 ttcccgatac aacgactgct gtcgctacac tggcagcggc tgctacgctt aatccgattg
    10681 ttacttttgt tgatgttttc ataagtatca gctccttttt atctatctcc atcatagcat
    10741 agacaaaaaa ataaacgaac taatccactc agtgaaatct caatccttta gggaaaaagt
    10801 tctttactgt tccttttact tgtttcccaa atccgacagg tatcttatct aatactagca
    10861 aaacccatcc ttgattccca tcacgttgaa atgtttctcc tgctgtgtaa gattgccatt
    10921 ccttttgagt gatcggcaag caaggtatat tctctatctt ttttgtagca agcgctaatg
    10981 catagctagg ttcaaatcga ttctttttga aatctcctaa atgcaaacct gtacgtacga
    11041 cttttaaacc atcgagagaa ggggctaact caggaacttc ccaaagatgg tcgttgaaca
    11101 ctaaaagtct tccagtagct tcataatgaa aatcatttga aaactccgtc cagagttttt
    11161 cctgctcttt tgtcatttgc actttacttt tttttcgtgt cttcttttct ttatgcatct
    11221 gattttgtcc atgaaaagtc agctttgcaa caaaatgtcc ttcaccctga tctttatgag
    11281 gccatatacg tattgttttt tccaaaccag ctacgcttcc ccattcagaa cgtcctgaac
    11341 taacggattg agtcaaagga atctcctcaa tcgttactgg ataattttct acaagccagc
    11401 taatgatctc ttcattttct tctggtgcaa acgtacaagt cgaataaatc agttgtcctt
    11461 tattttttag catttttatt gctgaagaca aaatctcttg ctgacgtttt tgacagtata
    11521 gcggagattc ttctgtccac tcttttattg catttggatc ttttcggaac attccttctc
    11581 ctgaacaagg agcatcaacc acgatccgat caaaaaagcc cgaaaaatga gggacaagtt
    11641 ctgcgggtgc atgatttgtc actattgcgt tcgaaactcc ccatcgttct atattttctg
    11701 ataaaatctt cgcacgtttc ggaaagatct cattcgtcac taataaccct ttccccttca
    11761 tttgagcagc taattgggtt gatttaccac cgggagccgc acacaaatcc aaaacttttt
    11821 caccgggttt tgctgcagca gctgttccaa cgatcattgc acttggttct tggctatatt
    11881 catacccagc ttgatggagg aaagactttc cattgacagt gccaagaaat ccctcattgc
    11941 tgtagggagc gggttgtaat tcttcgctat ggtaagtttg gaccatgtcc aatcctgccg
    12001 gttttagtgg attccaccga aatccttttt tcacgcttcc ttgttcaagc gcagaaaaaa
    12061 aatcggatgc ttcttcgcct aaaagaagac gatatttttt gataaattgc tgtggtaacg
    12121 ttgtcgcttc ctccttcatt ccaatgtttc gttagacatt ttacaatctt ttttacgaaa
    12181 aataaaggtg gctgatctac tttttcatgt tgctgcctaa ggtgactgtt cctttaagat
    12241 attttccaag tattcatccg tcacctcgtt tttatcccaa acagcgttca tcatttcaag
    12301 aaaatcttga gaagaatcat ctgagcgcaa acttcccaat gcattaagcc ataccaattc
    12361 aagcgacttt tcttctttga tcttcgtgtg acctttgaca tatgagaaaa atggccgctt
    12421 aaatagcgaa tccatgtcta aatcagattt tgcaatgatt tcacgaatac tttcatggat
    12481 agaaaaaaaa tctttcaata agtagacaat gcttaaaaca tcatcggaac tgcattcagc
    12541 taagtagttt tcaaaataaa cagtgatttc ttctaatatc ttctctttta acccgctgat
    12601 tgatttgaaa tgtgtataaa tcgggtatgt agaacaaccg cagaactctg ctaactctct
    12661 tgcagtaatt gacttcattc catattcttc cacgtatacc atacttttga acagcaaata
    12721 ttctttacta taatttgtta ctctccccat cattcttacc tccgtctctt ttcatctacc
    12781 cataaataaa taacataaat aattctaaaa atattattga taattatcaa aacatattta
    12841 gttatatcca ttaaaaatca atttttccgc cttaaaaaca agaatatctt gacaaaatac
    12901 cctaggaaat aaacaataga ataaacaagg taacaaaaaa attaaataac gttatataaa
    12961 aaagaaggcg acactctgtg agaataacac agactgaaat gactttccac tcacagtttt
    13021 tgtctaacta tatacaaaaa cagaataaaa aggaggtatt tatgctaatt gaagcaagaa
    13081 aaaaagagtt acttgttcta caaatggtca ttcaaaaaaa taattgttct ttacaagaat
    13141 tatccaatga cttacatatt ccaaaacgga caatcaaaga actgatccga aaaataaatg
    13201 tatcactcaa gcaattttta ttgattgatg aatttattta ttctaatcat aaaggagaga
    13261 tccaaaccaa agaaaccgat caaactaaaa agttagctgt tttttctaag ttaaaattat
    13321 gctacctaaa agaatccaat cgttttaact atttgatgtt actaatcaat tatccacaaa
    13381 ctgctgtacc aaaaaaatat ttacttgaac aactttatat atcgccctcc tatctcgaaa
    13441 aactgacaaa acagctcaat cattccttaa aaaaattcca tttacggatc ctatctgtaa
    13501 aaaattgctg tactcttgaa ggaaatgagt tatctatccg tctctatctt tacttttttc
    13561 tttcagatac atttggaggt aacgattggc cattttcctc acttgaatta aatgcactaa
    13621 aaatgaacag tacagatgag caaatagaaa aaatttcaaa acaagaaaaa aatatctact
    13681 tgctgttaac tttattcata ttacgaaaca aacaacaggc atattttcca aaaacttctt
    13741 ctgaaatcac tgaattgatg gacttattgc agcatacaca tgattattca gaatacttta
    13801 cttctttttc agtgagtcgc ccttgtgaag agacagaaac agagagagcc tactttaatt
    13861 attttttcta ttgttttttt ccaaatcaaa tctcttttcg acaaaaacag gagattggtt
    13921 ttttattttc cacaagcaaa aaccttattt gtcgacaagt cggtcaattg atgacttact
    13981 taactcaaca gtttcctctc aaccatttag aaaataaaat accaatttat ttttattttc
    14041 ttgtgttggc agttgttttt ttcgaactgt tgggtgaatc cagcagtagt ttttctacac
    14101 tttgctttcc agaagccacg tccaatcatt ctagttcttt tatacaggaa aagaaattaa
    14161 gagaaaaaat cgatacaatt ttttcaaaaa atggggaaag aaaccaaatc agtcaatacc
    14221 ttagtcaatt gatccaacac ttactacaaa cagaaacgaa agagcagtta ctcatccatt
    14281 tgcagataac caaacagttt acaggaaatt atcatcttca aagccatttg cagcagttat
    14341 ttcgcaaaga tgctgttaaa attaccgcat attcagaaca tgctgatttg atcattacag
    14401 atcagcatga gaaaatttca gatgaaacgg agactttcta tctggataca acgcacaata
    14461 cagtgttatt tggcaagtta ataaaagcgg ttcatgaact gtatatgaaa aaaggttatg
    14521 agaacaaaac aaagaggatg tgacaaaatt cttgtcacat cctcttatt
//
LOCUS       DAJSBN010000064        14184 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJSBN010000064 DAJSBN010000000
VERSION     DAJSBN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.065"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(143..1345)
                     /locus_tag="ONT86_002334"
     CDS             complement(143..1345)
                     /locus_tag="ONT86_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2353092.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="ONT86_002335"
     CDS             complement(1348..1542)
                     /locus_tag="ONT86_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR2353093.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="ONT86_002336"
     CDS             complement(1589..2437)
                     /locus_tag="ONT86_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HCR2353094.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="ONT86_002337"
     CDS             complement(2642..2992)
                     /locus_tag="ONT86_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353095.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3587..4033
                     /locus_tag="ONT86_002338"
     CDS             3587..4033
                     /locus_tag="ONT86_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR2353096.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="ONT86_002339"
     CDS             4657..5649
                     /locus_tag="ONT86_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2353097.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="ONT86_002340"
     CDS             5665..6549
                     /locus_tag="ONT86_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2353098.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="ONT86_002341"
     CDS             6598..8205
                     /locus_tag="ONT86_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2353099.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="ONT86_002342"
     CDS             8206..9690
                     /locus_tag="ONT86_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCR2353100.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="ONT86_002343"
     CDS             9867..10859
                     /locus_tag="ONT86_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2353101.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="ONT86_002344"
     CDS             10911..11780
                     /locus_tag="ONT86_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2353102.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="ONT86_002345"
     CDS             complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="ONT86_002345"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR2353103.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(13820..>14184)
                     /locus_tag="ONT86_002346"
     CDS             complement(13820..>14184)
                     /locus_tag="ONT86_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2353104.1"
                     /translation="RPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQET
                     LKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRN
                     FYNFRARIYLQQGLIFEN"
BASE COUNT         4341 a         2405 c         2927 g         4511 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tttggctctt
    13741 cgtcaaatcg tgttggtgaa gacaaatttg aataaaactt agtccgatat gcggcgcgtt
    13801 ctgtgcgccg catatttttt tagttttcaa agatcagacc ttgttgtaaa tagatccgtg
    13861 cccgaaagtt ataaaagttg cgataaccaa acgcaattcg tttaatcagc ttgatcttgt
    13921 tattcagccc ctcagtgacg ccgtttgagt gggacacctt aaaggcattt gaaatacccg
    13981 attggtagcg atggaagatc gtgaatttct ttttaaacga ctgaggtaac gtagatgaaa
    14041 ttgttttaag ggtttcttga aagccttccg tacctcgttt aacgaaataa tagcgtaata
    14101 attgacaaaa atgatacgca gttcgcaatt cttcgttgta gcttaacagc tcatcaacga
    14161 tttctgtttg actaattggt cgtt
//
LOCUS       DAJSBN010000065        14107 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJSBN010000065 DAJSBN010000000
VERSION     DAJSBN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.066"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            66..767
                     /locus_tag="ONT86_002347"
     CDS             66..767
                     /locus_tag="ONT86_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCR2353105.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="ONT86_002348"
     CDS             764..1885
                     /locus_tag="ONT86_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR2353106.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="ONT86_002349"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="ONT86_002349"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR2353107.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="ONT86_002350"
     CDS             3264..4172
                     /locus_tag="ONT86_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCR2353108.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="ONT86_002351"
     CDS             4207..4479
                     /locus_tag="ONT86_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCR2353109.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="ONT86_002352"
     CDS             4490..5536
                     /locus_tag="ONT86_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCR2353110.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="ONT86_002353"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="ONT86_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR2353111.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9233)
                     /locus_tag="ONT86_002354"
     CDS             complement(8547..9233)
                     /locus_tag="ONT86_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353112.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9211..9705)
                     /locus_tag="ONT86_002355"
     CDS             complement(9211..9705)
                     /locus_tag="ONT86_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR2353113.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="ONT86_002356"
     CDS             complement(9725..10945)
                     /locus_tag="ONT86_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2353114.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="ONT86_002357"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="ONT86_002357"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR2353115.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..>14107)
                     /locus_tag="ONT86_002358"
     CDS             complement(13021..>14107)
                     /locus_tag="ONT86_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2353116.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4555 a         2592 c         2919 g         4041 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatct
//
LOCUS       DAJSBN010000066        13852 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJSBN010000066 DAJSBN010000000
VERSION     DAJSBN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.067"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..902)
                     /locus_tag="ONT86_002359"
     CDS             complement(<1..902)
                     /locus_tag="ONT86_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCR2353117.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="ONT86_002360"
     CDS             1185..2153
                     /locus_tag="ONT86_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2353118.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="ONT86_002361"
     CDS             2266..2544
                     /locus_tag="ONT86_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR2353119.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="ONT86_002362"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="ONT86_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR2353120.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="ONT86_002363"
     CDS             3001..3756
                     /locus_tag="ONT86_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2353121.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="ONT86_002364"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="ONT86_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR2353122.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="ONT86_002365"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="ONT86_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR2353123.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="ONT86_002366"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="ONT86_002366"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR2353124.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="ONT86_002367"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="ONT86_002367"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCR2353125.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="ONT86_002368"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="ONT86_002368"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR2353126.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="ONT86_002369"
     CDS             8493..9035
                     /locus_tag="ONT86_002369"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR2353127.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="ONT86_002370"
     CDS             9058..10614
                     /locus_tag="ONT86_002370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR2353128.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="ONT86_002371"
     CDS             10628..11530
                     /locus_tag="ONT86_002371"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR2353129.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="ONT86_002372"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="ONT86_002372"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR2353130.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="ONT86_002373"
     CDS             12973..13395
                     /locus_tag="ONT86_002373"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR2353131.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4575 a         2474 c         3013 g         3790 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag acttttgtgg ctctttgtca ataaggactg atgagttgtg
    13741 caaaatcaaa tctgagtcag aatgaaccac attctggctc agattttttg ttatcttaca
    13801 ttgattaatt gattgatcaa aaagattcta attctcatgt tctcaaatga tt
//
LOCUS       DAJSBN010000067        13731 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJSBN010000067 DAJSBN010000000
VERSION     DAJSBN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.068"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..1452
                     /locus_tag="ONT86_002374"
     CDS             1..1452
                     /locus_tag="ONT86_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR2353132.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="ONT86_002375"
     CDS             1801..2433
                     /locus_tag="ONT86_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCR2353133.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="ONT86_002376"
     CDS             3021..4460
                     /locus_tag="ONT86_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2353134.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="ONT86_002377"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="ONT86_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5222..5437
                     /locus_tag="ONT86_002378"
     CDS             5222..5437
                     /locus_tag="ONT86_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353135.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="ONT86_002379"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="ONT86_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6097..7008)
                     /locus_tag="ONT86_002380"
     CDS             complement(6097..7008)
                     /locus_tag="ONT86_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2353136.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="ONT86_002381"
     CDS             complement(7027..8031)
                     /locus_tag="ONT86_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR2353137.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="ONT86_002382"
     CDS             complement(8028..10151)
                     /locus_tag="ONT86_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2353138.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="ONT86_002383"
     CDS             complement(10156..12603)
                     /locus_tag="ONT86_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2353139.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="ONT86_002384"
     CDS             complement(12590..12979)
                     /locus_tag="ONT86_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2353140.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="ONT86_002385"
     CDS             complement(13039..13542)
                     /locus_tag="ONT86_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR2353141.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13555..13716)
                     /locus_tag="ONT86_002386"
     CDS             complement(13555..13716)
                     /locus_tag="ONT86_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353142.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         4221 a         2710 c         2509 g         4291 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt g
//
LOCUS       DAJSBN010000068        13398 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJSBN010000068 DAJSBN010000000
VERSION     DAJSBN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.069"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(14..706)
                     /locus_tag="ONT86_002387"
     CDS             complement(14..706)
                     /locus_tag="ONT86_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR2353143.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="ONT86_002388"
     CDS             complement(1143..1712)
                     /locus_tag="ONT86_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2353144.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="ONT86_002389"
     CDS             complement(1786..2109)
                     /locus_tag="ONT86_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353145.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="ONT86_002390"
     CDS             complement(2263..3075)
                     /locus_tag="ONT86_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353146.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="ONT86_002391"
     CDS             3429..4277
                     /locus_tag="ONT86_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR2353147.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="ONT86_002392"
     CDS             4422..5363
                     /locus_tag="ONT86_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HCR2353148.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="ONT86_002393"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="ONT86_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ONT86_002394"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ONT86_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCR2353149.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="ONT86_002395"
     CDS             complement(9827..12013)
                     /locus_tag="ONT86_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCR2353150.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ONT86_002396"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ONT86_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCR2353151.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="ONT86_002397"
     CDS             complement(12235..12675)
                     /locus_tag="ONT86_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCR2353152.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="ONT86_002398"
     CDS             complement(12749..13288)
                     /locus_tag="ONT86_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2353153.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DAJSBN010000069        13328 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJSBN010000069 DAJSBN010000000
VERSION     DAJSBN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.070"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1230)
                     /locus_tag="ONT86_002399"
     CDS             complement(<1..1230)
                     /locus_tag="ONT86_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR2353154.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILI"
     gene            complement(1227..3416)
                     /locus_tag="ONT86_002400"
     CDS             complement(1227..3416)
                     /locus_tag="ONT86_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2353155.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            complement(3550..4740)
                     /locus_tag="ONT86_002401"
     CDS             complement(3550..4740)
                     /locus_tag="ONT86_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HCR2353156.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(5612..7243)
                     /locus_tag="ONT86_002402"
     CDS             complement(5612..7243)
                     /locus_tag="ONT86_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCR2353157.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(7257..8930)
                     /locus_tag="ONT86_002403"
     CDS             complement(7257..8930)
                     /locus_tag="ONT86_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCR2353158.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(8943..9260)
                     /locus_tag="ONT86_002404"
     CDS             complement(8943..9260)
                     /locus_tag="ONT86_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR2353159.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(9285..10685)
                     /gene="lacG"
                     /locus_tag="ONT86_002405"
     CDS             complement(9285..10685)
                     /gene="lacG"
                     /locus_tag="ONT86_002405"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCR2353160.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10922..11707
                     /locus_tag="ONT86_002406"
     CDS             10922..11707
                     /locus_tag="ONT86_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2353161.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            11710..12402
                     /locus_tag="ONT86_002407"
     CDS             11710..12402
                     /locus_tag="ONT86_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2353162.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(12676..13047)
                     /locus_tag="ONT86_002408"
     CDS             complement(12676..13047)
                     /locus_tag="ONT86_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR2353163.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         4104 a         2719 c         2187 g         4318 t
ORIGIN      
        1 aattaaaata tcgtttgcaa ttagctttct tcttcgaacc ttttcacctg attcagtaaa
       61 gtacaaaatt gctaacttga aaaataaact atgattattt accttaattg gtgatatatt
      121 tatagcatca aaattgcaat acggtaattc tttctttaaa tactcaccca gtgaaaacag
      181 aggatagtgt tgaggagcaa gtacacaaat atctttttca tgaatgccgc tttcaatctt
      241 ttcctttatt atatttttta ttatgttggg taaatcctcc ttagtaactt tgcaatcata
      301 acttataaaa ccacggtcgt ttttattatt ccctctgctt tcaatctcgt aaggacaact
      361 ctgaaaatta gagtagaagt ctactaatct ctgattagac ctgtaacagc catgtagcgt
      421 ctttgattga aaagtgtgac tagttagcat ttctagttct tcaactgatt tagctatacc
      481 atctaatgat ccataaattg cttggtcaat atctccaaca aacattgact gaatttgagg
      541 gttgttttga agtaatttag ctattacatt gtattgtaat tctctagtat cttgaaattc
      601 atccacataa atcgccctta tattactagc aatattacgt gcaacgttac tgtttacagt
      661 taaaatttta tacgctacta gtaaaatcat atcaaaatca atttctttat ttttttccaa
      721 catagtgtga tacatttcag aaacttgagt atactgaact tcattaattt taaaatcatg
      781 agaaagtgac aaatcaattt tgttcagttc atacgttttg agatttaaat tcagcatatt
      841 attaatttgg ctgacgtaag ctctttgagt ataatcatca ataattgtaa atccttttga
      901 tactcttgag agattcattt taaaaggata aataataaat tcaagacaaa actgatgtat
      961 tgtccccacc caaatattaa tatcatcatc tatactaagt aaacttaggc gttccttaat
     1021 ttcttcagca gctctattag tgtatgtgat agcaattatt ctttttagtg atccatcgtg
     1081 tttaattgct tggtaagcaa ttttccttgt aagtaccctt gttttcccac tcccaggaca
     1141 tgcatttaca taaaggttat cttcactttc cacaacatct ttttgttcta aatttaaatc
     1201 atttacgatt ttttcgaaga tatttgtcat tttgaaacct cagcataaag tatttttgtg
     1261 taattttcta tctgattgat tagatacaca acatcctcat ctttaccaaa gtactctaat
     1321 aaagaaatta ttgctatatt caaatcagct gtatcacttg tttttaaact agctgataaa
     1381 gctgacaatt ctgtagtttc ctcgtacaag tcgattgtat ggagtagaat ttttttataa
     1441 attgagagat ctactatttc ctgacatgca aaagcaatag cattaagaat atacttagga
     1501 attttgatta aaaagtcaat tttttcacct aactcaattg caaaccaccc cttaccaaca
     1561 tgattggcta gttttaacat cgctaaattt ctttctaaat attgttcact ttgtatttct
     1621 tctttataag cagttattgt ttttttatct ttgaatgcgt catctataat tggactgaca
     1681 taagttagaa tattttcttt attcacatct aaaaactcaa tttcaaatgt tgtattagca
     1741 taaaaacaat taacccaatt gttttcatta aaaagggagg ataatttttc ttttcttgct
     1801 tgacctttct cttgtgcagt gcttttatac aatcgatgtt ctgatgggat tgcctgttca
     1861 tctaaatcag ttacaatagc acactttctc tgtattctat catcgtcaaa caagctagcg
     1921 atatattcaa acgaagtact accaacatta atcaaaccta ttccaagttc gtctaagttt
     1981 ataccaaata atttttttac tatttgagga ataagtattt cttcagcatc tccttcaact
     2041 aagataacac ctttagaaaa aagcaatcca tttcttttaa catctaaata tctctctact
     2101 gcgtttatta gatttagatt gttctttttt aacttttctt tagcaaatga gttcagatta
     2161 tttgctggat tcataacgat agatttgcca ttaaacgatt ttaatacatt catccgagat
     2221 atttcagaca cttcagctaa atgtacagaa tgtgtggtca tgagaatctg agtatagttt
     2281 ttttgaagat ttaatccttc gaacagagtt ttttgaatat gatgatgaat atgagcttct
     2341 ggttcttcaa tcaacataat attaagtagt tctcttgaac gacacgcttc aaattcgact
     2401 attttcaaag ctaaataaat catatttaga tgtcctaacc ctagtaagtc taagtcgata
     2461 ttttgttctg gcttcattga aagaaatttt gctaaagaac ccatatcatc tgacatagtt
     2521 gaggaaagaa gaagattagg agaatacact gaaccaatta tgctatttag ttgccgatta
     2581 atattttcgc caattttcct aatttcagga acactggtaa tagtctcatt taattgctgt
     2641 ataattgttt ttaatgactc aacattatct gatgatattt ttgattcaat tgtttccatg
     2701 attcttttga ctgggtttcg attgtttttc atttctctaa gaacatctcg aagagcgtca
     2761 acataaacaa ccgaaatatg cttgaaaata tctgacatgt caattcttga gcccaacaga
     2821 gcttcatcgt cttcttcagg attactagct atattgtcat tgagtcttcc taccaattta
     2881 tcataatttt cattgataca gaaatttaga tttgatttag atgtgtagta aaattcatag
     2941 tcggatagac gaatagaatt tctaaattcg ttaaattgat gagagtctaa ttcagtggaa
     3001 atatcaaata attgctttct aatagctttg ttaggtctaa taaatagaga aatactacct
     3061 acacctttat ctgcatttga aatcaattct tctaagattg tttgactttc agaatttaaa
     3121 gaaatggaag cacaaatctc attgtcaaat tcttcttcgc tgattccttc aaatgtcgca
     3181 gagataataa tccaatgccc ttgccaccca ttttgaattc catgaaaaaa atctgattct
     3241 tttagtgttt tactactata gtaataacta tcatctaata agattctcaa tgcagtaaga
     3301 gcattggttt tccctgaatc attctcacct ataatagtat ttgtctcatt atcgaatcga
     3361 aagatttcgg attcataatt tctaaaattt cgaatttgaa gagtagatat aaacataagc
     3421 agatctccta actatttaat taatatgatt atatagtaag tttatcataa attatgttag
     3481 taaaagttct cattataagg gagcgtcaat aattttgtgt aaatgattct tccctactgc
     3541 agattgtttc taattctcaa tcagttggct tagcatttgt tcgagctcgc cttcggcctg
     3601 ttggaaacct ttatgactgc gacctaggaa tttttggttg taagtatcaa aagacgagac
     3661 taagaaacgt tccatcgact cttcattttg gaactgttcc ttgcggtggc tgtatttctt
     3721 gatttgttta ttaaaggatt caatcaagtt cgtagaataa atactcttgc gtatggccaa
     3781 ggggaaatca tagaaagtga gcaagtgatc gttcgaaaga atcgattcaa ccacttttgg
     3841 atagctggtt ttccattttt ccgcaaaagt accacgtgct tccaacgcca cctctttaga
     3901 tgaggcttga tacaccattt taaaatcatc acagacttcc ttacgatcat cgacacgcac
     3961 tttatgactg atattacggg aaacgtgtac acaacaatgt tgaaaacgag ctttgggata
     4021 gaaccgacta atcgctccta ccatcccctt taagccatcc gtgatgaaca ggaggacatt
     4081 tttcaaaccg cgctcttgaa ggtccaataa aatttcctcc caaatcgtga tggattcagt
     4141 cggtgcaatc gcatagctca atacttcctt tgatccgtcc gggcgaatgc caactgcgat
     4201 atgaatagct tccttggcga cggttttccg ctttaacgga atatacgttg cgtccatata
     4261 aatagcagca taacggtcat gaagctcccg cccattaaac gccgttacct cttctgtaaa
     4321 tgattttgtt atattggaca tggtttgggg cgtgtagtaa tgcccataca ttttctcaat
     4381 caagtctgcg atttccgaca tggtaatacc ttttcggaag aggtgaatga cggtctcctc
     4441 taacgtgtca ttcgtccgtc tataagcagg aacagtctgt tgcttgaact caccattgcg
     4501 gtcgcgcgga atctggagat gaagttcccc atactcggtc ttgaccgtac ggtcatagga
     4561 gccgttacga gaattacccg tgttaaaacc aatgcgatca tacttttcgt aatctaggaa
     4621 agccgttaac tccgttttga gaagcgtatt gactgctttc tccaaatgga cacggaataa
     4681 ttcattcaaa tcgcctttgt tcgctagagt ctttagaatt tctgtagtaa aatcgttcat
     4741 agggaagtcc tctcttctgt gaatgtgttg tggtaactct attctacaga agggacttcc
     4801 ttttttctat ttacacaaaa tattttacac tctccattat aacaattggg ttaaaaagaa
     4861 aatagatagt attctacctc atatttattt gaaagtaata tataaaagac gttcaaattc
     4921 gaacgcctct cgttaaccaa atattggatt cagaatctag ttttcatata taaacagagt
     4981 atgattaggt gcaaaaattg cacttttatt gcacttttat ttttatttat agtgttttac
     5041 tgttctgtat attgttttaa ctcccttgat agtatcgcat tctgttttat acactttacg
     5101 ttgaaaacag agtgggaata aagagccaac ataatatgga gggggaatcc ctataaatca
     5161 acattcttca atagctaaaa tgcttgattt tcattaaaaa taagatggtt ccctaccaaa
     5221 agagactata atataagtta atttgtttca ttcttatcat aaaaagaagt tctcgaaaaa
     5281 cgaactgttt tccgagaact tttttctatc catctaagtg ctcaaatcat attcacgacg
     5341 gaattctaag gttacccttt cttaaacaaa ctttatgtta tctgcacaac tttttgcaat
     5401 tttttcattg cttcttacga aatcgttgaa ctctctttag cataatacaa agtgtagatg
     5461 aagtctggtg ctttactcgt tttggtaaaa atgactggaa atcgtttctg gttatttttg
     5521 gcaaaggcga ggtgcaggtt cgattcctgc cttctacgct aacgatgtgg ctgaagggaa
     5581 cctccgatct tgaatcgaaa cgatccgcag tctattgttt caccaatgaa acaaaatcaa
     5641 cgttcgtcca agcatttcct gaagccttgc tataaacccc aatttcacaa acacctgttg
     5701 taattggaat gtctttgatt gtaatttttg tccattcact tgttttaaca accgcagtat
     5761 ttagttccgc tccaccataa tttttgatat ataactgatt agtatcaaac tctcccccag
     5821 acattacata ggcactcaat gtgtaagttc ctttgcttat gccactaaca ttttgataag
     5881 ctgatgcctc gtatgcttga tcctgccaaa aagttagttt gtagtcccca aaaaaagtgc
     5941 ccgcttccgt tttgatcgta cccttagcgg ttccttgttg aaaccaggtt tgccaattgg
     6001 aaggactggt cgttggtcca tcgctttcga aacttgcatt tttaactaga tttatttgcg
     6061 cccctgcttg tttaaacgca cgcaagcttg gcaaggcgtt tccagcaaaa tcaaacatcg
     6121 cttggttgtc ccattcgttt cccatagtgg attgaacact gagataattc atacctgctt
     6181 gtgttgccca actcacattt ccgttgtacc aaagaggttc ccaatagaag aagcccgccc
     6241 cgcgattatt cggtactgcc tgaatccgat cggcaagatc cagcaaatac tctgcttgtc
     6301 ctgtcggtgt tggttcatat ccccccgcag ctgcttcggc ttcaccaaaa gcatttggca
     6361 tagtatcggc attcgctgtt gtatgcccat aagctgtttc aactacgatc acttctttac
     6421 cataccgttg gctgatcgca ttcatattgg tttgaagttc attcattgaa ccgtgccaat
     6481 acggataata cgacactcca attaaatcaa atgacacacc tcgactcgtg atttcatcaa
     6541 accaccaagt aaatgctcct gtatcgccac catcagctaa gtggagcatt actggagttg
     6601 ttttaggttg agtgtctcga acagcttgga taccagactt caaaaaagca gccaatcgat
     6661 caaattctcc cccgccttct ccccaagact tgccataagg ccagagcatt ccgctattca
     6721 gttcattgcc gatctgaacc atatcaggat aaacgtccag ctgcttcatt tccgacagaa
     6781 cgttctttgt atagtcgtac aatgccgtat tcatttcttc aaaggtcaga ccttgccatg
     6841 ctttaggaag attctgtttc ccaggatcaa cccaaaaatc actgtagtga aaatccagca
     6901 gcaccttaag tcccagattt tttgctcgtt ttgcaaggtc aatcgtcgtt tccagatcat
     6961 ttgtccctgc tccataactt tctccattac tattataagg atcctgccac aaacgcagtc
     7021 gaacatagtt gacaccatta ttttttaaaa tctctaaggc atcttttgaa atatcgtctt
     7081 cataatattt tgcaccagat ttttccatat ctgacaggat cgaaatatct gctccgcgaa
     7141 taaattcgct ggcagatgct tcaacagttt ttgaaacagc ccccaaagcc cctaacacag
     7201 ctgcaaataa aacagcttta cgcaaccaaa ttcccatttt cattatggct cctcctttat
     7261 tgaaattggt tcagaataaa ctgaacagct ttttcaggat ctctcgttaa tccgatatat
     7321 tctgctcctt ttgtcgcaac cagcttgatt cctaagcgat cggtgtcttc tttgatatca
     7381 tcatagtaag aattgacttg cggtgctaag accaccatat caaaatcctg cagcatttcg
     7441 taatgagaac cataagcacc cgccattgca ctgatcggtg ctttcgattc agatgcacct
     7501 ttttttaaag cattagctaa catcgcactt gtccctgcgc cagcacaaag taccaacaca
     7561 ttgatttctt tatttgcatc gctgataact tgactcgctg ccacaggtac ttcctcacgt
     7621 acatcggcag aagcaccttc ctcggttgcg atcacctctg ctttttgtgc ttctttctct
     7681 actaagacag catcgtatgc tttgcaaaat ggtagataga tcaggaagtc aacgacgatt
     7741 agaaccgctg ctagaacaaa agctaagata cccatgcccg ttcctagaac caaaccgatc
     7801 ggtgctggag ttgcccaagg caaaacgtac ataaagctgt tcatgcccaa tacatcgaca
     7861 aaaaacttga acaggcaaac gtttacaaca ggtgttaaca aaaatggaat aaaaaaataa
     7921 gggttcaaaa tcatgggtgc cgcaaatagc aatggttcgt tgacagcgaa acttactgga
     7981 ataaaggaag ctttcccaac tgccttcaac tgcttagact tagcaaagag taggaagata
     8041 aaaggaacaa ccaatgttgc cccagttcct cccatagttc ctacaaaatt ccctaaaccc
     8101 accgtcagta ctttagaagc ttgttcaccg gaattgaaca attgcagatt ggtttccaca
     8161 ttggcataga taattgccgc aattgccggc tccacgatcg acggtccatg aacaccgaca
     8221 aaccaaaaca gcgccatcgc tccccagata atagcgattc ctaagtaccc gtcagctgct
     8281 gtaaatagcg gctgcagcac cgtaatgatt gcttctgcaa atgagatttg gaagatttgt
     8341 cgaatgccta aatcaattaa aacggccgca aatactgaaa aagagaaagg aaaaatatct
     8401 cggaaggttt gcgaaatcgt tccaggaact tctttaggca ttttaatggt aatatctttc
     8461 agcacacaaa acttatataa attaactgtt aaaaatgcag cgacaaagga agccagtagt
     8521 ccctttgtcc ccatatattc cgttgaaaat ccaccttcaa tcggagagac gcttaacaac
     8581 aggaaactta ccacagaggc cagcatcaca gaggtggtgt tgattacttt gtttttcggt
     8641 aagcgacgat tcatcgaatc agccaagcag cgagcagtgg ttgctgaaac gagtaaccct
     8701 acaacaccca tcgaataatt atagactttc catagccatg tggagacaga ttcaggccat
     8761 tggtaaccaa aaatctcagg aaccgcagca atcaaaatga aaatgcttga aaataaaata
     8821 gctggcatcg ctgttaaaaa tccgtcacga acggctccta aataactatt acgtgagatt
     8881 ttttcaaaaa atggtttccc tttttcgatt tgtttaatga ttccatccat agcgttcttc
     8941 tcctatcctt gattctgtct gtacagtaca ataaagtctt gaatcagatc tctcagcaat
     9001 aaagtggtca ttaaatgatc ctgcccatgg ataaaaatga atcccatctc catctctttc
     9061 ccagatgctt cttccgctaa aatttttgtt tgcgaattat gtgcaagggt taaattttcg
     9121 tcggcttctt tcaacgccga atcaacatgt tcaaagtttc cttgtcttac ttctttcaat
     9181 aatttcaata atgtactgcg agcgtcgcct gaatatgcaa cgatctcaaa accaagcatt
     9241 tgtaattcct ctttattcat cttttcttcc tccattatca cttattaaat aatcgttttg
     9301 gtttcactga ccaacttgta ccagtaagct gattcttttg ggtaacgctt ctgggtttca
     9361 aaatcaacat aaaacaagcc ataccttttg ttatacccat ttgtccatga gaaaagatcc
     9421 ataagcgacc acaaaaaata ccccttcaca tttaccccgg ctgttatcgc cttgcttaag
     9481 gattccaaat agactctcaa atagtcgatt ctcggctgat ccataataat gccatcttca
     9541 aactgatctt tataccccat cccgttctca gtgatataga ttttgttgta atgtgggtaa
     9601 tcgcttttaa ttcgcagcaa caagtcatat agaccttctg gataaatcag ccagtcccaa
     9661 tcagttcgag gaattccttc tttataaata cgttctccga ttcccttcac tttataaaca
     9721 gaggtccctt tttcacccgt accgttatga tggattgcat tttctccatc gtaagctttg
     9781 acaaaatggc attggtagtg attgatccct aagtagtcat tacgcgtgga tgcttttttc
     9841 aattcaacga aatcttcttc aggaaaatga tagctggcct gatttgcttc acatatctca
     9901 tcaagagcag tcaaggtttc agttgaataa tagcctaaat aggtagcatc taataagaaa
     9961 cggatcgaca atgcatcgtc taaaaaggca gcatgcttat cttccggcgc gtctgttgcc
    10021 gcatatttcg tctctaatga gtgaactaca ccaatttcac ccggaagttc gttttctttg
    10081 aaatagttca ctacacgggc atgagcaacc atcatattat gaagacaggc gacgatcttt
    10141 gtaaaatcat atttgattcc tggtgggaaa acacctaaca aatattgatt ggttgcaact
    10201 ggatagattt cattaaaggt actccacacc ttgacttctt tgaattcatg aaaacaaaaa
    10261 atggcatagg aaacaaatgc ttctattgtt tctcgattca aaaaatctcc atggtcaaac
    10321 aaacgtttag gggtatcaaa atgatgcaac gtcacaaacg gcgttacgtt tcttttctta
    10381 cattcttcga acacacgatg ataaaaagcg attccttctg gatttggttt cccagctcca
    10441 tcaggaaaaa tgcggctcca agcaatggat aatcttagac catttacacc aaaacgttca
    10501 cacaattcaa tgtcctttgc atattgatga taaaaatcac ttgccggatc gggactgaat
    10561 cgcccttgtt cctctaaaaa gtcatcccaa gcaacagcgc ctttgccacc ttccttagtg
    10621 gctccttcaa cctgataggc tgctgtggct ccgccaaaaa taaaatcttc tggtaattta
    10681 agcatgtaca tctctcctca aatctataat cagttttcgc tctttctaac tgattgcatt
    10741 gtaaacgcat ccaaaacatt tgtcaacaaa aacaaacaca aataatcata aaaaccagaa
    10801 aacagcaata aaatctattg taacaaacat aaaaaccacc ctaaatgttt acttttgttc
    10861 gaattagatt gactgttgtc cgtttatatg atagattatc cttaaattag aggtgaagac
    10921 aatgttgaaa aaagaaagat tgcttactat cgtagaaatg gtcaataaaa aaggaatcct
    10981 taccgttaat gaaatcatca atcaattaga tgtttctgat atgaccgttc gcagagattt
    11041 agatgaatta gaaaaatcgg gcaagctgct gcgtgtccat ggaggggctc aaagtctttc
    11101 ctacacttta gatcaagaac tttctcacac tgaaaaagcc actctgcaga ttcaagaaaa
    11161 aaaagaaatt gctgccacag cagcctcctt gattcacgag ggagaaacga ttttcttagg
    11221 accaggtaca acaatcgaac tattggcgac gcatttactg aacaagaaaa ttcggatcat
    11281 tacaaataat tatccagttt ttgatattct tgctcaagca gatggaccag aaattctact
    11341 gattggcggt gatttccgga aaaataccgg cgcatttgtc ggaccgatca ccaacgacaa
    11401 tttaagaaga ctaaagttta caaaagcatt tatcagttca aatggcgttc acaacgaagc
    11461 gatctctact tacagtaccg aagaaggaga agcacagcag atcgctctaa ataattcccg
    11521 taagaaatat ctactcgttg ataataaaaa attcaatcga gatgattttt atgtgtttta
    11581 taatttgcat gatttcgatc tgctcattac ggatcgccag atctcgcggg aggttcaaga
    11641 gcactatgca caatatgtcg atctgcaaat tgctgattca accgaaaata ccactatagg
    11701 agcgtaacta tgaaagggat tatatttgat ttcaacggaa caatgttttt ggactctcat
    11761 ctgcatgaag ctgcttggct gcatatgata catgatcata caaaagatgg tttatcagac
    11821 gaggatattt taaaaaatat tcatggtcgg acgaatacgg agattttgaa acatttcatt
    11881 tcagagcatt tgaccgcctc tgagatcgcc gtattgtcag aagaaaaaga aagctattat
    11941 cgtatgcttt gcttgcaaaa tgaaaacgag cttcagttga caaaagggct tgaagcgact
    12001 cttgaccagc tgactgacca acagattcca ttaacgattg ccacggctac tgtcaaagag
    12061 aatgttgctt tttattttga tattttcgat ttagcccgct ggtttgactg ggacaagatt
    12121 gtctatgacg acggctcttt ccctgggaaa ccgcaaccgg atatttttct taaagcggca
    12181 gacaaactga gcttagctcc ggctgattgt cttgttatcg aagatgctta ctcaggatta
    12241 cttgctgcca aacgtgcggg gatcggaaca attattgcaa ttgatccatt cggtaaaaac
    12301 aaaacagtat ttgaacaggc acagctaaat aaagatggaa ccatcaaaga ttttactggt
    12361 ttttggacag agcacctgtc tgccacatcc gttaattctt aaacgattca actaactacc
    12421 aaagaaaagc aactgaaatg cacacctcct tttataaaaa actgccagtg aaacgacatg
    12481 aaagatacac tgctatatcc ttagtgaaat atagcagtgt atctttgact ttttcattct
    12541 tttaaccata tgacgcgttc atctctatct ataattaaca ttaaaaatag tgttttcaca
    12601 ttatttctca aaccaatctt aaaaatcatc aggagtactt ttttgatata cttttattaa
    12661 tgctgggcgt aatggttaaa agatttccac tagttcctct ccaccaagct gagttgaaaa
    12721 atgcttatct gtaaacaaca tagacaaaga ggaaataaac gtttccgtat ctttctctgt
    12781 cccaaggata ttaattaatt ttcctactag gtaagtatca ttaaacttaa gatctgcctt
    12841 cacatcaaaa gttttgataa aagcttttat catgcgtaca attttgtctt tatcgccata
    12901 attgatttca ccactcatat caatatagtc actaatccct gaagaaataa tggcaactgc
    12961 ttcttctttc gccttatcag ataattcatt taatgactgg gattgattga tgacttcttg
    13021 agttttatct gttacgtaat ctctcattat cctatcggca tactccgcaa agtaatcttg
    13081 aaaaaattct tctacagggt aaccaacttc gttttcccaa ccaggagaaa tttcattatc
    13141 agtacttatt aaatctatta aaaaccaacc cgttctctga gtgttgattc tataatattt
    13201 ttaatttcct atataaatca aaaatattat agaatcaata agctaattaa gaaacaaact
    13261 ttagaaactc tagaagaact tctgaattta tatagaagtg tgtagctact tactaaatat
    13321 acaaaagt
//
LOCUS       DAJSBN010000070        13285 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJSBN010000070 DAJSBN010000000
VERSION     DAJSBN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.071"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            174..1913
                     /gene="yfcC"
                     /locus_tag="ONT86_002409"
     CDS             174..1913
                     /gene="yfcC"
                     /locus_tag="ONT86_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR2353164.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2070..3254
                     /gene="iadA"
                     /locus_tag="ONT86_002410"
     CDS             2070..3254
                     /gene="iadA"
                     /locus_tag="ONT86_002410"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR2353165.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3365..3568
                     /locus_tag="ONT86_002411"
     CDS             3365..3568
                     /locus_tag="ONT86_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR2353166.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3669..4331)
                     /locus_tag="ONT86_002412"
     CDS             complement(3669..4331)
                     /locus_tag="ONT86_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2353167.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4344..5846)
                     /locus_tag="ONT86_002413"
     CDS             complement(4344..5846)
                     /locus_tag="ONT86_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2353168.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6394..8073
                     /locus_tag="ONT86_002414"
     CDS             6394..8073
                     /locus_tag="ONT86_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR2353169.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8200..8679
                     /locus_tag="ONT86_002415"
     CDS             8200..8679
                     /locus_tag="ONT86_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCR2353170.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8898..9578
                     /locus_tag="ONT86_002416"
     CDS             8898..9578
                     /locus_tag="ONT86_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR2353171.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9586..10917
                     /locus_tag="ONT86_002417"
     CDS             9586..10917
                     /locus_tag="ONT86_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2353172.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10932..11894
                     /locus_tag="ONT86_002418"
     CDS             10932..11894
                     /locus_tag="ONT86_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCR2353173.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12005..12343
                     /locus_tag="ONT86_002419"
     CDS             12005..12343
                     /locus_tag="ONT86_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCR2353174.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12422..13129
                     /locus_tag="ONT86_002420"
     CDS             12422..13129
                     /locus_tag="ONT86_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCR2353175.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4239 a         2338 c         2911 g         3797 t
ORIGIN      
        1 atataaatgg aaagagaaaa aaataatcat acataggaga aaaaaaacaa aatagaaaaa
       61 agtgattttc taataaatta ttcatgtgga aatgtcattt ttactgtata attcatttga
      121 ttatccattt tttattcatc aaaattactg aaaaaagaaa ggaagaaata aatttgaaga
      181 aaacaaattc ttcaggaaaa ccgaaacgcg acttcactaa attatcgaca ccccatacgt
      241 atgtcattat ttttggtgta gtcatctttg catggatact gacttttgtc gttccagcag
      301 gaaagttcag cacgcaagat attgaataca aagatgcaaa tggcgaaaca agtactcgta
      361 ctgttttacg tcaagattcc ttccgttatg cttacgagct ggataagtcg tatgtgtttg
      421 atcaattaga agaactgcaa gatcatccag cagaacgtga aaaactggat gttccggaaa
      481 aaggattgga aaaagtcatt gctgacggag aaaaaaatct tacgcaggaa aaactagatg
      541 aaatctcctt gacagatgat gtgctttacg atcagtacgg cgaaaatatc tatgatactt
      601 ctaaaaaatt gcataaaaca gccaaaatat ggggaacaga tgattttggc ggctttggtt
      661 tcttaaattt tgtttttgaa ggactagtat ctggagataa gtatggatct gctgtaggga
      721 tcgctgcatt gatccttgtt gttggaggtg cttttggtat tattatgcgt accggagcga
      781 tcgacgcagg tatctatgcg tttatcagta aaactaaggg actggagcgt ttagctttac
      841 cgctattatt ctttgctttt tcattcggtg gcgctacatt cgggatggca gaagaggtaa
      901 ttccattctc aatggtgatg gttccatttg tcattgcgtt aggatatgac tcgatcgtag
      961 cagtaaccgt gacttatgtc gcatctcaag tcggaaatgc gacctcttgg atgagcccgt
     1021 ttagcgtagc ggtggctcaa gggattgctg gaatccccgt gttgtctggt gcgacattcc
     1081 gattgattat gtgggtagtt gttactgcat tagccgctgg ttatatgatg atatatgcag
     1141 aaaaaatccg taaaaaacca gaaagttcgt tgacttacaa atcagatgat tatttccgtt
     1201 ctcatatcaa aaagacatct gatgaaaata aaaaattcat gcttgggcat aaattgatct
     1261 tattagagat gttagttgtc cttgtctgga tcgtttgggg cgtgactcaa aaaggctatt
     1321 acattccaga aatcgcttct caattctttg tgatgggatt agttgctgga atcattgctg
     1381 ttcttttcaa attagatgga atgaaagtca atgacatcgc ttcttctttc caatctggtg
     1441 cagctgattt agctggtaca gcaatcgtag taggtatggc caaagggatt cttcttgtct
     1501 tgggaggatc tgatgcatct gttccttcag ctttgaatac gattttgcat gggatcggga
     1561 cggcgttgac cggtgttcca gctgtcattg gtgcttgggc gatgtacatc ttccaaagtt
     1621 tgttcaactt ggttgttaca tcaaattcag gacaagcagc tttgactatg ccaatcatgg
     1681 ctccactggc agatttagta ggtgtatcca gacaagtagc agttcttgct tatcagcttg
     1741 gttcaggttt tatggacgct tttacacccg tatcagccag cttgattggt gtgctaggtg
     1801 ttgcacggat cgaatgggca aaatgggcaa gattccaaat caagatgcaa ggattcctgt
     1861 ttgttttagg aactattttc atcatgattg ccattgcaat aggtctgcag tagtactgga
     1921 gacctgtaaa taaaaaagta atccaaacag tagataagga ctccaagaac cgtcatttta
     1981 cggtaaaatc aaaggagtaa agatcctatc gtttggatta ttttttaaga tcctatcatc
     2041 agttatactt aaagaaagag gaaaaatgta tgaaaatcat tcgacaaatt gaagtgtttg
     2101 caccagatta cttaggaaaa atggatgtac tgattgccgg tgggaagatt ttggctgtag
     2161 aagaacagct agaaggcggc tacgaaggag tggaagtaga agaactttca ggtgagggaa
     2221 aagtactaac cccgggtttc attgactgtc atttccatat tttaggtgga ggaggagaag
     2281 gcggttatca aaatcgaacg ccagaagtca cattgagcca attgactaca gctggtgtaa
     2341 cgacagttgt cggttgtttg ggaacagacg gagaaggtag agatatgact gcgttgatca
     2401 gcaaagcgaa aggtctggaa gcagaaggaa tctccactta tgtatacgaa ggttcgtacc
     2461 gattgccggt aaaaacagta acagattcta tcatcaaaga ctttttgaca attgataaaa
     2521 ttatcggaat cggagaaatc gcagtatccg atcaccgttc ttctcagcca agttttgaag
     2581 agttcgctcg tgcagtagca gatgctcgtg taggaggcat gttatccgga aaagctggga
     2641 tcatcaatgt ccacttaggc ggaggaaaaa gaaagctaga gctattgaca cgaatcgtag
     2701 aagaaacaga aattccgatc acacaatttt tgcctactca tgcaaatcgc acaccagaat
     2761 tatttgaagc ttgcgtggca tttgccaaac gtggtggcac gattgacttc actgctagcg
     2821 aagatcctga tttttgggag aagacagatg gtgaagttcg cttcagcaaa gcactgaaac
     2881 gattgatcga ggaagatgtt tctttagata atttcacgat gacctcagat ggacaaggaa
     2941 gcttgcctta ttttgatgaa aacaatcatt tcctagggtt aggagtagga agtgccaagg
     3001 cattgctagt agggatcaaa gaagctgtgc aaaaagaaag tatccctttg gaaatcgcct
     3061 tacgagctat aaccagtaat ccagcacgta tattgaaatt ggataaaaaa ggaaaaatcg
     3121 aaattggtgc tgatgcagat ctgtgtatct tggataaaga aacgcttgat attgatacag
     3181 ttatcgctaa aggagaaata atggtccaag aaaaagaagt caaagtatgg gggactttcg
     3241 aaaaaagttt ttaatcaaca gctctaacta tcttttttag aaaacgagcg ttctgtttgt
     3301 ctgtatctgt tgttgatgtt accctgaagg tagatcaata aacaaactaa gaggaggaag
     3361 cacaatggca gatttaaaag gacgttctaa agatatgaaa gataaagtag tcggtgaagg
     3421 aaaagaagca tacggcaaag caacagacga caagagcaaa gaaacagaag ggaaagcaca
     3481 atcactaggt gctgacatca aagaaaaagc tcgaaattta ggcgacgaag tcaaagacaa
     3541 gttttctcga aaagacgaaa aaaaataaaa accacttatt actaattgaa tcaagcaaat
     3601 aaaaaatccg aaccatatct gcgagacaga tatggttcgg attttttatt tgatcattat
     3661 cctgtttttt aaacttcgat tttttgaaat tttttatttt ttagttcaaa gacttcatca
     3721 cagctttttg ctacttcaat ctcatgagtc acgatgataa tacagcgatt ttgttcatgg
     3781 gcgatttctt ggaaaagttt gacgatatct tgtgaagttg tttcatctag atttccagta
     3841 ggctcatccg ctacgattag ttgatgttca cagcagatag cacgaacgat agcgactcgc
     3901 tgctgctgac ctcccgaaag tttggtcacc tgtttttttg ctaatatttc atcaattcct
     3961 acacgagcca gtcgatctaa agcaacttgt cgataattct cttgcttggt cttagtgatt
     4021 tccattgctg ctatgacgtt gtctagagcc gataaataag gaagcaagtt gtaagcttgg
     4081 aaaataatcg agacatcatt tttacgataa cttcttaacc cgattttgtt cagtgaacga
     4141 tcttgatagt atacctttcc ttcttttggt gcatctagtc cagaaataag cgaaagaaaa
     4201 gtcgtttttc cagaaccact tgctcctaaa atagcgtaca tttttccagc ttcgaaattc
     4261 agttctacat ctttataaag cgcatcttgc ggatcttgat accaaaatcc caattgttct
     4321 gtccgtaaca tcaaattcct cctttagttt aataggattt ttcttgggtt taatcttaat
     4381 atccctatag aagctaaaaa gacagaaaac agactgattc caaatgctac tccggctaat
     4441 agtcccaatt gtgcaggttt gacattgatt tccaattcgc tgacttgatc agaaacagca
     4501 aatggattat tcgtggttcc ttgttgacca tcgcgttgac cgccttgttc ttgtctgtta
     4561 cctggcattt gtccaccgcc aggagcttgc tggttttgtg tcgtggtttc tgtctgttgg
     4621 tcaagtagct gctgtcccac ggcattcgcg acgacgtttc cgctaagcga agcaactcct
     4681 aatgcaagaa tcatacaaat agccacttcg gtaaacaatt ggagaatgat tttacttctg
     4741 gattctccta aagatagcaa gacgcctaac tcatgccgac gctcccgaat ggtaatcata
     4801 atgatcagag taagtatgat aataccggct acagccacta gtaaaacaac atttttagaa
     4861 aaacttgcca cgttgtttaa aggttccaac attgactgat acatggaatc atttgattgt
     4921 aagctaaatg tatctgtgtc gatcagcttc tcagcttctt tgagaaactc atccatttta
     4981 tctggatcag atagagtgta aatagcagaa tctatcgtgt tatcgtcaga tgtcccattt
     5041 agttcatttg caaaagtgta agaagtgtaa agtgtattcg acggattcat gaagttgaat
     5101 ttcataccca tactgccgct tgtttcgctg gattcataaa ttcctttgat cgtcatttca
     5161 taagtggtat cttcatcttc agtactcgtg attgtgaaag tatctccgac tgccaggtca
     5221 tttgtttctg ctagagtaga atcaatgact acattgtttg tgcctttgtc gctgtcatct
     5281 attccttcac catccgtgat ttttgctgtt ccatcagaaa aattactatt ttgtgcagtt
     5341 gctgacacac cactgatttg aaagtctgct tgagtcattt gcggattccc gcccattcct
     5401 ccagcaggtt caccgcctgc atcagtagtt tctgaactgt cttcacttga atcagaacta
     5461 gtgatggcag ttattccatc ttttgccata gctgaggcgg aactttcaaa ggaataactc
     5521 tttacattat ctaattcagc tattttttcc gcgtccgtta aggaaactgg tgtaagagaa
     5581 aaactgcctg gatctggacg ttcttcagtg gtagaaccgc ttgaagaagt ttgttcctgt
     5641 tttttaaatg aagcttctcg attggcactt aaagtcactg ttgctccaac ttctttttgt
     5701 gcttgattcg ctgcttcttc tgcagcgctt cgaatcgtga ggccagctag cacaaaaata
     5761 agaatggctg tgaacacggc aaacaataac aatgaacgtc cccattttac ttttgtactt
     5821 ttccatgcac gtttcataaa attcatattt tgcatccctc ctagtagatg catttgataa
     5881 tagacaacct atcttaaagc tagcttaaat cttatgctgt ttgcacacca tcaacatgag
     5941 gttctgtata tataaaaagt cactatagtt attgcaaaaa atgtggtaaa atgaaaaaag
     6001 tatgtttcaa accgacgaag aagaaatgaa agaggaatag caaaagaaat cctagatgct
     6061 acagagggat ttttacgtgc atagttgttc taacaattgc tgatactagc gatttagttt
     6121 atttgttgta taataatggg gaacagctga atacttatat aagtattctg gctatagcgt
     6181 ttcgcaaaag atccattttg ttaatgaagt atatccaaaa taaaatgccc atccaactgt
     6241 cagaacgcgg atgtgctggt aattttcctg ctgattctcc ataagttgag aaatagtgat
     6301 tttgcatatg cttcattgga atggctcata tatttagata aagaagaaaa tcgacatctt
     6361 taaacttgga tgtcataaga agaggtgaag tttgtgagta ccattaaaat tatcccatta
     6421 ggcggcgttc gtgaaaatgg aaaaaacatg tatgtcgcag aagtgggcga ggatattttt
     6481 attttagatt gtgggctaaa atatcctgaa aatgagttgt tggggatcga cgtcgtaatc
     6541 cctgatttta cgtatttaga agaaaacatt gatcgtgtag ctggtgtctt tttaacacat
     6601 ggacatgcag atgcgatcgg ggcacttcct tacttgttgt caaaaatttc tgtaccagta
     6661 ttcgggacag agttgacgat cgagttagca aaattgaatg tcagtcgtaa tgaagcagcc
     6721 aaaggattta aggatttcca tgtggtagat gagcatacag aaatagactt tggtgaaaca
     6781 gtagtgagct ttttccgtac cactcatacg ataccagatt cagtggggat caatctgaaa
     6841 acaaaagaag gtagtatcgt ttatacggga gattttaaat ttgaccaaac ggctatccca
     6901 atgtatcaaa cagattatgc acgattggcg gaaatcggca aagaaggagt cttagcctta
     6961 ctaagtgaat cggcaaatgc agaaaatcct cagcctgtag cttctgaagc ccaaatagca
     7021 gatgaagtat ttgatacgat caaatattgg gaagggcgta tcatcgtcgc ttgtgtagca
     7081 agtaatttgc aacgagtgca acaagtattg gatgcagcag aaaaagcaca acgaaaagtt
     7141 gtcttgactg gtcaagattt cgaacgaatt attcgcacag cgatgcgttt agaaaaactt
     7201 caattaccaa gtgaagatct gcttgttacg ttgaaagaca tgaaaaacta tgatccagaa
     7261 gaactgatca ttttagaaac tggccgaatg ggtgaaccca tcaagtctct gcaaaaaatg
     7321 gcaaacggta cacaccgtaa tgtcaagatt gaagaaggag atttggttta tatcactact
     7381 acgccaagca tcgcgatgga aaccatcgtg gcgaaaacgg aagatatcat ttatcgagct
     7441 ggtggaacag tgaagttgat ctctgagaat atgcgcgttt ctggccatgc caatcctaat
     7501 gacttgcaat taatgatcaa tttgatgaaa ccaacatatt ttgtgccagt tcaaggagag
     7561 tatcgtgaat tagctgctca tgcagatttg gctcatgcag tagggatgcc atacaaaaat
     7621 atctatatta ctggacgcgg agacgtgctg gaatataaga acaagcgtat gagtgttgca
     7681 ggaactacaa gtgcagaaaa tgtaatgatt gatggattag gcataggtga tatcggaaac
     7741 attgttttac gtgatcgaaa aatcttgtca gaagacggga ttttcgtagc ggttgtcact
     7801 atcagtcggc gagaaaagaa aatcatttca aaacctcaaa ttacttcacg gggattcgtt
     7861 tacgtaaaag ctagccgtga tctgatgaaa gaaagcagca atatgattga aacaatcgtt
     7921 gagaaacatt tagaaagcaa tgattttgag tggagcaaat taaagcaaga catccgagat
     7981 caattaggcc gttacttatt tgaacaaaca aaacgccgcc cagtgatctt gccagtcatt
     8041 atggaagcta ctcaacgtcg aaataaaaaa taaacaaaaa gagtaagatg aacctctcta
     8101 tagagtgctc gtcttgctct ttttttgcga aaaacaaata atttttgaat acagcatccc
     8161 tcgctttata ataaacgata gaataaaggg agtgaatcaa tgatgagtca aacaaaagtc
     8221 gaacgttatt tagaagaaca aaagatcagc tatcaaccac ttgattttag tcatgtggta
     8281 gaagaatctt tcacagaaga actacaaaaa agagggattg atccagcttt gatatgtaaa
     8341 actctcgtag tgaaaggaaa caaaactggg gtgatcatcg cattgattcc tttgaacgat
     8401 cacttagatt acaaaaaaac gagacaagtg acgaaagatc gtaaagttgg ttttcctgga
     8461 atggattttg tattaatgca tacaggatat ccccacggag caaacacccc tattggaata
     8521 aaactcgcac atcctgatta ttgtttttta gcagacagtt ctttaaaaga aaaagaagaa
     8581 gtaattgtgt caagcggtga aatcgggaga agccttcgga taaaagtagc cgatttagaa
     8641 aagatcgtcc atcctattta cgctgattta attagttaag ggacgagaag agaagctaag
     8701 cagagcagag acctcaaact tgagattata tctgcttggt acttggtgca tcctgtatta
     8761 tctgttacaa ttgctttgta catactgtac tatcaatcca atgaaaatat atttagcagc
     8821 tatatttttt cattgatgtg caagtgatga aatcgttttt aataaaaaag atacagagaa
     8881 aacgcgaggt gtaaaatatg aaaattacgt atcatggaca ttcctgttta acaattgaaa
     8941 tggaaaatgg ccaacgcgtg atgattgacc cgtttattac aggaaatcca ttgacagact
     9001 taactgcaga agaagtaaag acagattggt tgctgattac tcatggtcat agtgaccaca
     9061 taggagatat gattcctatt gcaaaagaaa atcaagccac aattatcagt attgttgaga
     9121 ttgctgatta tgcgaacagc agaggagtag attcattcgg tatgaatatc ggtggaaaac
     9181 atgcgtttcc attcggcgtg gtaaaattcg tacatgcaca gcatagttca agctatgaag
     9241 tagacggtat cgttcagtat atgggcgagc ctagcggaat tattattcaa gcagaaggaa
     9301 aaacgattta tcatgcaggg gatactgctt atttcagtga tctaggattg cttgcagaag
     9361 aattcgatat tgatgtcgct ttcttgccga ttggcgataa ctatacgatg gggccagaag
     9421 atgcagcaaa ggctgcgcaa acaattcgtg ccaagaaagt agttccaatc cattacaata
     9481 ctttccctgt cattgaacaa gatcccgaat cttttgtaga gttactggaa gatagtgaag
     9541 gaaaaataat gagcgtctcg gaaacgctgg aagtgtaggc agaaaatggc gacaaaacat
     9601 gatttgattt tagaatatat tgaaagttta cctgttggca atcgaatttc tgtaagaagc
     9661 atcgcaaaag aattgaatgt cagtgaagga acagcgtacc gtgcaatcaa agatgcagaa
     9721 aatgtcggac tagtttcaac aattcaacga gtagggacca tccgtatcga acgcaaatta
     9781 aaaaaacata ttgaacgttt gacatttggc gaggtcgtca ggatcattga aggggacgtt
     9841 ttaggcgggg cggcaggatt agacaaggtc ttgaataaat tcgtcatcgg cgcgatgact
     9901 aaacatgcca tggaacgcta tattacgcca ggatcactaa tgatcgtcgg caaccgggcg
     9961 gaagtccaaa aattagcatt ggaagatggc gccgcagtat tgatcacagg aggattcaat
    10021 acgactgctg agattgcaaa gctagctgat gatcttgaaa tgccgatctt acgcacctct
    10081 tatgatacat ttacagtcgc aaccatgatc aaccgagcgt taagcgacca attgatcaaa
    10141 aaagatatca tgcttgtcag tgatatctat atgccagctg aaaagactca ttatttgcag
    10201 cagacagata cgatttctga ttatcagcgt ttgtcagaag aaacgcagca ttcgcgtttt
    10261 ccagtcgtca accgccatca tcgtttgatg gggattgtta cggcaaaaga tgttttaggg
    10321 aaatcgccaa accagatcat cgatcgggtg atgacgaaag aaccaatcag tgtaaagaaa
    10381 acaatgagta ttgcctctgt tagccatcaa atgatctggg atgggctaga agtcatgccg
    10441 gtcgtttccg atgacttgac attggaaggc ttgattaccc ggcaagatgt aatgaaagcg
    10501 atgcagcttg tccagcgaca accgcagatt gcagatacga tttcggatca gatatctgga
    10561 aatatccata cgattgatac tgaccgtgaa ggaaatcgtt tggaaaaccc taagttcaaa
    10621 ttcgtagtag caccacaaat ggtcaatggc gttggaacca tttcttttgg cgtacttagc
    10681 gaaatcattt ctgatgtcgc acaaaaaaca atggtgatga accaaaaacg aaatatcttg
    10741 attgaacaag tgaacctaca ttatttgcgg ctgatccaat tggaaagcga attagacatt
    10801 cgtccacgtg tattggaaat cggcagacgt tccgctaaac tggatatcga agtgtttatt
    10861 gaaaacacca tcgtttccaa agcaatcgtt gtttgtcaag taatggaacg ttcttagaaa
    10921 gcaggagtga aatgacagta cagcaagaaa tagtagatac aataaaaaaa tacgataaaa
    10981 tcattatcca caggcatttg cgccctgatc ctgatgcatt aggatcacaa gtaggattag
    11041 ccgaactatt acgggaaagt ttcccagaaa aagaaatacg tcaagtaggt gaaacgattg
    11101 aagggcttcg ctttttagca gaaatgcaag aagttgaaga tgtcttctac caaggagcgc
    11161 tagtcatcgt gactgataca gctaattcac ccagaatcag tgatcaaaga tatcagttgg
    11221 cagaaaaact gatcaaaatc gaccatcatc ccaatgatga cccttatgga gatcttgtgt
    11281 gggtaaatac aaaagcaagt agctgtagtg agatgattgc agaatttgct ttgatgtttc
    11341 ctgaatcttt aaagatgaac gttaaggctg cacggttact ttatgcagga atcgtcggag
    11401 atacagggcg ctttttgtat ccctccacga cgtcccgtac attagagatt gcttcgatat
    11461 tgagaaatta tccatttgat gcaagtgctt tgaacagaga gattgaacaa atgccgatga
    11521 aagtcgctaa actttccggt tatctttatc aaaatatcca ggtggatgaa aatggtgcag
    11581 gaagaataat ccttccgcaa gaaattcttg ataaatatgg aatcgatgat tcagagacag
    11641 cttcgatcgt ttcacttcct ggagtgattg acgaagtgct ggcttggggg atttttgttc
    11701 agcagccttc agggtattac cgcgtacgtt tacgttctaa ggggccgatc atcaatgaat
    11761 tggctaaacg ccatcatgga ggaggccatc cattagcaag tggtgcgaat gcaaaagatt
    11821 tagcagaagt agaggaaatc tataaagaga tccaagatat atgccaatcg tataagagca
    11881 gaaaaacaga atgatacttc gttcgaaaac gtgccttttg tagtaaaaat cacaaatgta
    11941 tcttttcaag atcttctttt tccgttacaa tagtgctcac taaagatttt aaaggagaaa
    12001 gaagatgaca ttaccaaatt gcccagaatg tggctcagag tacacatatg aagatcgagg
    12061 cttgttgatt tgcccagaat gcgggaacga atggaacaaa gaagaacaag aaactgtcat
    12121 agaagacgga ttagtggtat tagatgcaaa tggaaaccgt ctgttagagg gcgatagcgt
    12181 gacagtgatt aaagacttga aagtaaaagg tgcgtcaggt gccattaaac aaggaacaaa
    12241 agtcaagaac atccgtttag tagaaggcga tcataatatc gattgcaagg tagaaggatt
    12301 tggaccgatg aaactgaaat cggaatttgt gaaaaagaat taaagaatat tcaaggtctt
    12361 ttcacattct tttcctacta ctaatgataa aataaagtaa acgaaaagga gtgaaacgaa
    12421 aatgtatggt tatggaatgt atggtttttt agatccgaca tttcttttgg tcattcttgg
    12481 attagttatt tccggaattg cgtcagcgta cgtcaatagt actttccgaa aatacgatca
    12541 agtgagaagc aaaaacaatg tgactggtac acaagcagca caatatatat tacaaagtca
    12601 acagataaat aatgtcggtg ttcaacaaat tgccggagat ctcacagata actacaattc
    12661 aggtaataag atgttgagtt tgtctcaagc aactgcacag tctacttcag ttgctgcgat
    12721 aggtgtagca gctcatgaat gcggacatgc ggtacaagat gcttcgggtt atgtcccatt
    12781 gaagattcgt gcagcacttg ttccagcagc taactttggt tcgatgattt cgttcccctt
    12841 gattatggta ggtgtattgc ttagctggaa ctctacgtta atcaatatcg ggattttcgc
    12901 tttttcttta gcattacttt tccaattggt gacattacca gtcgaattta acgcgtctcg
    12961 gcgagcaatc caaattttga gtgaaggcgg actactgacg gaagaagaag taccgatggc
    13021 aagacacgta ttatttgcag cagcactgac ttatgttgca gcagcgcttt cgacattcct
    13081 gcagctgttg cgcttgatct tattgttcgg cggcaaccga agagattaaa ataaagaagt
    13141 tgtgaaatca gcgctttgat ctgagctaat accgagagtt agctgattga ataccattta
    13201 tttggaataa gggctctttg tcaataagga ctgatgggtt gtacaaaatc aaatctgggt
    13261 cagaatgaac ctcattctgg ctcag
//
LOCUS       DAJSBN010000071        12975 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJSBN010000071 DAJSBN010000000
VERSION     DAJSBN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.072"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            107..313
                     /locus_tag="ONT86_002421"
     CDS             107..313
                     /locus_tag="ONT86_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HCR2353176.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(515..1516)
                     /locus_tag="ONT86_002422"
     CDS             complement(515..1516)
                     /locus_tag="ONT86_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2353177.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1521..3434)
                     /locus_tag="ONT86_002423"
     CDS             complement(1521..3434)
                     /locus_tag="ONT86_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR2353178.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3602..4108)
                     /locus_tag="ONT86_002424"
     CDS             complement(3602..4108)
                     /locus_tag="ONT86_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2353179.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4268..4708
                     /locus_tag="ONT86_002425"
     CDS             4268..4708
                     /locus_tag="ONT86_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR2353180.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            4867..6207
                     /locus_tag="ONT86_002426"
     CDS             4867..6207
                     /locus_tag="ONT86_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2353181.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(6276..7433)
                     /locus_tag="ONT86_002427"
     CDS             complement(6276..7433)
                     /locus_tag="ONT86_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2353182.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7436..7792)
                     /locus_tag="ONT86_002428"
     CDS             complement(7436..7792)
                     /locus_tag="ONT86_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353183.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(8090..9064)
                     /gene="sgrA"
                     /locus_tag="ONT86_002429"
     CDS             complement(8090..9064)
                     /gene="sgrA"
                     /locus_tag="ONT86_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HCR2353184.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9260..10099)
                     /locus_tag="ONT86_002430"
     CDS             complement(9260..10099)
                     /locus_tag="ONT86_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR2353185.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10284..11171
                     /locus_tag="ONT86_002431"
     CDS             10284..11171
                     /locus_tag="ONT86_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR2353186.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(11755..12450)
                     /locus_tag="ONT86_002432"
     CDS             complement(11755..12450)
                     /locus_tag="ONT86_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR2353187.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(12434..12832)
                     /locus_tag="ONT86_002433"
     CDS             complement(12434..12832)
                     /locus_tag="ONT86_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR2353188.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3892 a         2628 c         2010 g         4445 t
ORIGIN      
        1 tttcaagttc gctagatgat cattttattc acatcctaac aaacttgtca catttataga
       61 ttcttaatct atttatcata caattataaa aaaggaggtg ctgcttatga aagctatcca
      121 tatttctttg ctttttttcg ctagtgcatt gattttattt gctattcatc tttgttccca
      181 gccttatatc aataacaacg gaatattagt ggaaccagca ttcttttgct taccactcgg
      241 ctttttgtcc cttcttgcct ccgcaggctt cggatttgtt gcttttttta aacgatcaaa
      301 acaacaaatc tgacttataa aatttacgta aaagagatcg tgacaataat cgtgtgatat
      361 tcccaaattt cgaataaaag gtgccccttg ccatctctaa ctcctcagtc acaagttaca
      421 atattcataa accatgaaaa gcttgtttat tgaaattgct ccttgtgact tggagctaag
      481 tgacagatta caacctcttt tgcatcataa gactttactc cttgaaaaac attactaact
      541 tttgatatgt ttcttgcgct tgtttattgc tgaaatcaaa ctgatattca tggttcaaaa
      601 cagcattttt ctccgcataa tctacttttg tgactttcac ccccgctttc tcaaatacag
      661 aagttacttc ttccatttgt ttcgtaaacg tatttgtatt tccatccgtt aagaatgtgt
      721 tttgcgtcca acgatccgca tgtctgacca aggctaaact tttgaccttc tctgattgtt
      781 caaagtcctt attatcaaag taagcccatc cacatttttc ataaagaaag ctagagattt
      841 tatcacttac ttgagtaagt gcagaaggat ccaacagacc agacaaagaa ataaacttcg
      901 taatctgctt gttctccaaa gtcggatcaa gatgatcagc ttttttaatt tcttcatttt
      961 gaagtgctaa caaatattct ccactaattt gtgcacctgc cgaatttcca ccaatcgaaa
     1021 tattcgacca atctgcttga tcttttatct cttttttagc tgcttgaatg gcttcgttca
     1081 attggataac tggtgtagga tgttttgctt ccggtgatcg ttcataatct atatttacga
     1141 tgatttgctg cgtgtctgaa catagatatt ccaaataatc acgacaatca tttttatccc
     1201 ctcctacata tgcgcctcca tgcatccaga aaagaatcgg taattttttc ttttccactt
     1261 tcggtgtgta gatatccata ttattatttt tgtactttga aggataggat atttcttttt
     1321 ttatatcgat ttgttcgatt ttttgctgga aatcatctgg tcgggcatat acttgttctt
     1381 tttcacttgt gtcaaattgt tttctaacaa gcaagctaat cggctttggt gtgatttgat
     1441 taatcaaaaa tacagtgact aaaataacta cgatgataac tggtactgca atcactattc
     1501 gcttgaacca tttcattcat ttaccctctc tttcttcgcc aaatccatgt gataatcgtg
     1561 ctaacagata aaaatccacc tgaataaatg agaagcgaat gtttcttttc tccagttttc
     1621 ggcaacattc ttttttttgc atctgtcttt ttttgtactt tcactgtcat ctgaatcggt
     1681 tgcgtatcat cacttgattg atcctctcga ggttcattct ttcccttatt gtctgcattc
     1741 tctgtcggat cttgcttttt tggtggagct actgggattt ttttgtaata gaaaataata
     1801 gttggtatcg tctcattaaa aatcccttca gtcggtcctt caacatttgc taattcgtat
     1861 ccttcgaatg tcttttgctc tgtcatataa ttttctccat aagctccgta aagctggatt
     1921 tcgtcctggt ctttgagtag acttttattg ttttcatcca catactttac gtttatctga
     1981 tcatggaaca ctactttcaa aaagtccgtt acatgtttcg tgtaggcagc atggtcaact
     2041 tcctgtgcct ttccatgccc tgctccttct actaataaaa gatctttata ccctcctgat
     2101 aactcatata atttttgcgc gttagacaca ggtactacat catctgctgt tccatggata
     2161 atcaatttag gcaatgaatc tgacattcca atcgtaggta gagcagcttt tgtatccaaa
     2221 tgcatctcct gatcaaaata gtattgtttc aaaaggccca taaataaatc ttcatgctct
     2281 tgatccccaa tgatgtctaa ctgatttttt atggcatccg gtagtgcggg ggctactgtc
     2341 tggctgtacg taaaacgtag ttgttcatca attgatgcaa atccgcaatt ttcaataatt
     2401 cctttgacat tgacaggaat atctttcgct aatacagaca tcatcgtagc tgctcccatc
     2461 gaaccaccat aaagtaaaat cttttgagat ggccgctctc gaacttcttg atttatccag
     2521 taaaggacgt catctgactc atattgtcca taggtcacat aatcgcctcc gctattacca
     2581 gtcgctctgg aatcaacaaa taataaatta tacccggcct tataaaaatc attgtattct
     2641 tctgtaacga tcccgttatt ccaattccct cgaaatccgc catggatcat gactgtttta
     2701 tctgaacctt gatcgacata atatgcatat acatccatca ttttgccatc ttgtttgttt
     2761 agtacagttt gatgaagttg ttttgtattt ggatagaagc ctgtgttttc tgtaggcgtt
     2821 gtcccatcat ctgtacctcc aagcatcgac atcaacacac tgttttttgc aaaagcgata
     2881 tcgtacaata gataagtagc aatcagatgt ttgataatct ctttatctac accataatca
     2941 ttaacagcaa gctcagtgac taataactct attaattttg acttgatgtc ttctgttggt
     3001 ccattaagta tttctgtcat ctgtttaccg aagtaattca aatattcttt accattgaaa
     3061 ttttcacctt taccgatatc atcattttct gtcccattct ctttgtcact tactttagca
     3121 gaagaggatt cgtcgtttga catttttgta gctgttgctt cttctgtttg ttggttatct
     3181 gtggtagcgg tcgtttcttg atctgtgcta gtaacagatg tctcaaccgt tgtttcagta
     3241 tttgtttctg tttgtgaagt actcgatttc gtttcatttc cagcatcagt agtttctgtg
     3301 gtttctacct cccgagattc cgattcggaa gaaatgctct ctccatatac tgtccattgc
     3361 gggacgcttg tacttacagc aaataagatg accaacgctc cctttaatac ttgttttgtt
     3421 ctcaagctat tcactccttt ttatgtttct aggagaatta tatgtaaacg caaacatttt
     3481 ttatacaaaa aataaatggt ttatcccata aaaatagaac aggattattt tctcatttta
     3541 cgtaataaag cagaatggat aaaccagtat ccattctgct ttttttaaac gaagaaacaa
     3601 cttattgctc caaaaatgtc agaaatcgtt tatcccaaat tttctcgtaa aaagcgattg
     3661 tttcctttgc tttcataaaa ggattatcat aagttgtcgt tgctcctcta cacatcataa
     3721 gctggtatcc taatccatga gcaaacttca cagtcgtatc tatacaatac tctgcttgtg
     3781 ccccacataa ttcgatcgaa tgacagtcca attcagttaa taaaacactc aaattagtct
     3841 tataaaatga attcgcatgg gtttttctca caaaataact gtgctcagga atagataatt
     3901 ccgacaatag ctcccatggt tttgtttgtg gcactaattg agcatcttcg tgttgaacga
     3961 aaataactgg tttcccaatc gtttcgtatg ttttgattct tttattcact aatgctgtca
     4021 ctgtttctaa ttcgtgtatc ggcgaagaat caaaacatac ccctttttgt aaatcgatca
     4081 ctactattgc gtcggctgtg tttgacatac ttgtgccttc tttctttttt cttgatttaa
     4141 tagtatttct tatctagcta gttccattat acgtgacaac attttcttat tttacacctc
     4201 taaatggcac ttttcgacta tttcttcatg ttattatttc ataaatactt agctattgga
     4261 ggaatgtatg atgattaaat tgtatacagg ttctggctgc tcgtcttcaa gacgcgcaaa
     4321 acgttggctt atcgtccatg atataccgtt tatcgaacag aacttaattc gtcaaccact
     4381 aaccaaaaat gaatttatcc acttactaaa attgacaaac aacggcgtga ccgaaatgct
     4441 ctctactcat tctattagtt ataaaaatct tgttcacaga atagatgaac tcagtttgag
     4501 tgaactatat attgagattc gtacatcccc ttctttattg aaaaaaccga ttatctacga
     4561 ccaatctgca aaacttttga ctggatttag cgaagataaa atcaggatgt tcctttctaa
     4621 aactaaaaga aagcaagagc tcaacgaact cttgctttct ggtcatcttg tcgattttaa
     4681 agaaaataat ctttttgcaa tgtcttaatt ttggcataaa caatctgctt ttctggatct
     4741 ataatatcta gtgttggtga cgccttctgt tgccaatact ttttcttttt tctaataaac
     4801 gcaaacagac accttgaaat cctttagaat aaagtcgacg aaaaccattc aaaggagtgt
     4861 ttcaagatgt cctattcaga gtttaccaaa gagttactag atattctaga cctaaatctt
     4921 acctttcatg aagatgcctt tcgcaaagag cgcattaatg acgaaacatg ttttgttttt
     4981 gatggcacat tgacctatca accagaggaa tgcttccatt gccactatca aaataagcaa
     5041 acgatcatta aatggggctg gaaaaaagta tccatcttat tgaatgatgt cagtaactac
     5101 aagaccattg tgcggattaa caaacagcgc ttcaaatgta aacactgtgg aaagactttt
     5161 ttagcagaag attcagttag cgatcgtcgc tgctccattg cccgcagagt caaacaagcc
     5221 attcttgagc tattaagtga gcctatttct atgtctttga ttgcacgtat gaagcacatt
     5281 tctccgacca ctgtcattcg aatacttcgc agccttcggc ctaaaaccgt ttctttgaac
     5341 cagccgttac cagaagtggt gtgtttcgat gaattcaaat cggtgaaaaa cgtctcggga
     5401 gccatgagct ttgtcatgat ggacggcaag acccatcaac tgattgatat tgtggaaaat
     5461 cggcaactca atcctttacg cgactatttc ttgcgctacc ctagaaaagt ccgtgaacaa
     5521 gtacgtctag tggtttcaga tttctataca ccctatcgca cattggtgaa agagtgtttt
     5581 ccgaatgccc gaatcgtcgc cgatcgcttt catattagcc aacacatcgg tcgggccttt
     5641 accaaccacc gaattcaagt aatgaagacc tttaaaaaag gggatcgtcg gtcgaagcac
     5701 ttaaagaaat actggagact acttcaaaag aatgcgtggg aattgaatgg tcagcatcgc
     5761 tactggcgtc cgtcatttcg agatcatctg acagaagcgg agatagtcga tcggctatta
     5821 tattatgatg actccttaaa gcgaggctac gaagtctacc aggtttttct ttccgctatc
     5881 agaagacaag atgttcctga attcgtcgcg ctcctaaaag aagactacaa ggagttgcca
     5941 gagcactatc aaccagtgtt cacgaccttc aagaaatatc gaacagaaat caaacgagcg
     6001 ttgcgagttc cctattccaa cggccctata gaatgtttga acaaccatat caaagtacta
     6061 aaacgaattg cctatggctt tagaaacttt caaaactacc gagaacgaat ctttttatat
     6121 cgaggaaaat atttcaagaa aactaagaac actacccaac tgactaaagc cagaacaaca
     6181 acacggctag acaagatagc tgtctagccg tgtgagtatc tcgattattc tattgaattt
     6241 cttcaccaac actcattgac gaagagccgc ttttcttaat taacgaaatc caatttttcc
     6301 aatatggatc tatttttctc attcctgatt tttatgattt cagctttcag acgcgcccaa
     6361 tcattttttg taggttgtcc attccagtaa aatatctgtg tattcgtttg atcatatgcc
     6421 tttttgtcga taggataatc gctaatgaca atatcaggta actgatcagc ggatgcacta
     6481 tattttattg ggcgttcagt catagataat atccttttct ttgcccataa ttcctgtgtt
     6541 ttaccatact ttgattgaat atagaatatg acaggttgtt cgtgttttat aaaaatatcc
     6601 ctgactaaca tacagtattg aaaaatcaac cgtctgttct tttcataata agtattaagc
     6661 tctggaattc tgcgagctac cccactaaaa aactgttgca tgattggata catcactgga
     6721 aatatatgct gaaaatctct gtcttctgct gattttccaa aagcatccac cttctttttt
     6781 gtcccaaaaa cccttgaata caaatggatc attgccaaat tgacatatag ataatttttt
     6841 tctgaagttg taaaacaaat cttgaagttt ttttcaaaat tttctacaat agacgattca
     6901 atccagcccc aactttcttg tactttatct ggagtatcca acaaatcttc ttccaaatag
     6961 gtgttcatta caccaaacat aaaataaagg taagcttgct ctgttggagt gatgtgattc
     7021 atcgaccaga agtttgcttc aaaaaaaggc tctagtatgt aatgcgtaaa ctcttctttt
     7081 gttatcaatg gatgcacaaa tttgttcatc tcttctggga catacgtaat cgagtggcct
     7141 tgtttcattc gttgcaacga aattcttagt aggctgatcc atttctttct atctatacta
     7201 cgcgtatatg gcgcatgttt cgcaaatttt tctaatagtt gacgtttatt ttttatactt
     7261 gataatatat cagaatgatt ttcgtcgaat gactgccaat acatcaagtg aaaaaagtat
     7321 cggatacggt attcttctcc ttctatcgga ttaccttttt tcaagctgca agataaatga
     7381 tagtttttca aaatagtatt aagttctctt aattttcttt gaatcgtacg caaacttata
     7441 taattttttg actaagattt tctaaggatt gataagtacc tttgtaaagt gaatccagta
     7501 aaatataacc aatcgttccc tctaaaagta tagaataaac tttactcagt aaaaactgag
     7561 aagaaaactc gacttttatc atcttgtttt ctgaaagaaa agataattta tattctggat
     7621 acctttcgca aaacgattct gcttgttgaa tcaccgaatt catcttagcc agggtgtagt
     7681 ttagatctaa agcgatatcc ttcaccacat ataatttctc gtctctttca taacaatagt
     7741 tcaaaaattg atatacttgc ctttcattga tatcaataaa ttcttcaaac aaagtacata
     7801 ctccttttta tctattattt agacttattc cgacagctac atgaataacg tacaaataga
     7861 ttcgttactg atttttcaga gatactacca tgcgcagtgt gcattgtcaa ttattcttgc
     7921 tcttattttc catttgtgtt cctgttattt cctgtttctc aaatatgtta gtaaacaaaa
     7981 atagatctgg caaagctaca acttcgccag gcaatacagc actctcgaat gaaatattca
     8041 ctcaaaaatc tatcgtttta ttcaatttta gatctgttgc ttttattctt tatttcactc
     8101 gatggaagag agaacgaaaa tagcttgctc ctacaatcaa aatgagtaac acacccatcc
     8161 atgtaattag cttgttcaag gttctactac cagtttctgg taaatcgctt tttctattat
     8221 ctgtttcttt cgctttttct tgatgatcaa ttccacgaga aacaaaatca ggctgtttta
     8281 gcacactttt taggttgctc cctgcatttc tcttttgttc cttagttggt atgataggta
     8341 ccaaatcaag ttcattattt acttttcctg tgctgctttc gctgctcgtg cttcctgtgc
     8401 tactctcact actcatgctt cctgtgctac tttcactact cgtactctcc gtactgcttt
     8461 cgctgctcgt gcttcctgtg ctactttcac tactcgtact ctccgtactg ctttcgctgc
     8521 tcgtgttttc tgtgctactt tcactgctcg tactctctgt actgctttca ccgctcgtgt
     8581 tttctgtact gctttcacta cttgtgctat ccgtactgct tggcggtcca acaactgtcc
     8641 aactgcttgg tttcaaattc tccgtgatcg tcatctgtcc atcataaaaa acaatcaaat
     8701 caactttatc tttactcttg atctcatcaa tcgatagttc tgcagaaatc attattgtat
     8761 actcaatacc agcggtccat tgtacgtctt ttaaataaac ttccccaata actttttctg
     8821 aacttccatt accatcccaa ccatatgtta gaacagcatg tacatcacta gcattcacgc
     8881 ttttgtttgg tttgactgga atagtaatga cttgttgtgt catgtcataa acaatccgat
     8941 cagttttcac agtaaatgaa agattctcat ttgcccgttc atttgcagaa gtactgattc
     9001 caatcattgc tgcaattata ctcagcccaa gaataacaaa taatacggtc gctgttttct
     9061 tcatttctat cactctccat ataattacct tctatttgat aaaggaatta tataaaatga
     9121 aaatgatgag aacacgacag tcagttgtga aaatctaaca aaagatgtcg tgtattcgct
     9181 tgaatttgtt tactttttcg tttaatcaat gacagactta aaaaagcaag accgatatca
     9241 gccttgcttt cttcatcttt tattgataaa cagcagtgta tttcttcatc cgctttgttt
     9301 tatatgcata gagtaacaaa ccaacaatca agactaacaa gaataatgta gttgatatag
     9361 atgcttcagc cccaaagttt cccccagtaa ttattgtatt ttgcgattgg atcagagtgg
     9421 agaaaaaacc ttgttcttgc gttccgctga ctggtatacc caaaatagct ccttgtgtcc
     9481 aattccaagt aatatgagca ccaatagccg taaataaact tcctgtactg tagataagtg
     9541 cgctgaaaaa gcaacctgct aaaaagatat tgaaaactgc cagaaatgtc atgttaggat
     9601 tcaatccgtg caaaagagca aataatgcag cagatccaat cattgctatt aacggaccaa
     9661 atctaagcat ctgatgctga aaaaagccac ggaataaaag ctcttctgat gcccccactg
     9721 caataaataa aataaaactc gttatcagcg gcacaatgtt tgcgtttcct gaatctaacc
     9781 gatacgcccc cgtcaaatat aatggactag taataagcag aaacactcca attccaacaa
     9841 cgagacctcc cgctaaatac ccccagtctt ttcctcgaac agaaacacca taatcttcta
     9901 ttctttttcc ttcgacctta ctagcaagaa ataaagctag taaaaaaaca gctaaatgtc
     9961 cgaccgtacc ggcaattgct ccaatccatt cactatttcc aataaacata tccgcaagtg
    10021 cattattgac aagaattcct aaaacaaaac caactaagac tattaaaaca gatccaatca
    10081 accgaataat cctgatcatc tttcatccct cctttatatt aagtttaaca acccttttct
    10141 ttcttgtctc gtgaaaacta tgtaaatgtt ctccatcata gagagaatag atttttacga
    10201 actaagaata tctacagcaa ccttacgaaa atgaaagact gtctccctaa ttcttctgtt
    10261 agactgtttt taataaggaa aggatgggaa attttttgaa aaaacaacta ctaattatgt
    10321 gcctttcagc atgtgcaatt ttactattct caggttgtag caagtccaat gaaacgattg
    10381 caacgtttaa aggtggaaca cttacagttc aggattttta tgataaccca aaagttaaat
    10441 caaataatca aacactatta aaaaaaatga ttgtcttcaa agcatttgat gatgtatacg
    10501 gaaaagagat ttcttcaaag gaagtaacaa aagaatataa tgaacaaatc aaaaaactcg
    10561 gtccaaacta caaggaacaa ttaaaagcag ttggccaaac agaagagaca tataagaaac
    10621 tctttaaaca gatgctggct ttccaatacg gtttgaaagc caatgtaaaa ttaacagata
    10681 aagatctaga cactgcttgg aaagaattct atccagaagt tagtacacag atcattctat
    10741 tttcgactaa agaagaagca gataaagcaa aaaaagaagc aaatgaaggg gaaaatttct
    10801 ccaagcttgt tcaagcttat ggaaaaaata aaacattaaa ggaaactgat ggaaaaatga
    10861 attttgattc aaccaatcca gagattccta ctgaagtaaa aaaagcagca tttaaactaa
    10921 aaaacgggga agtttctgat atcattcctg taacagatcc aacgacttat cagcaaagct
    10981 attatctagt aaaaatggta aaaaaacagg ataaaggttc taataaagac aaatacaaat
    11041 ctgagctaga aaaaattgct accgaagcaa aaactcttga tacggaattc atggataaaa
    11101 caatccgtaa agtgatgaaa aaagacaatg taactattaa agatccttat gtaaaaaata
    11161 tattcaaata aacaaatttg acccaattgg ggatgtggca aaatttgaga agtgattttt
    11221 ggaaattcct tcttattttt gaggcaaacc acttttgcca caacttcatc ggggcctttt
    11281 tttatttgta ccaaaacaaa tcactggatt tgttacaatc atgaggatat aatttaaaaa
    11341 agttttttat ttctatcaca tgaatcaatg cgccattaag tagattcttt catgcttccc
    11401 tattttttca cttgccgtat cgcttcaaaa acaactatca taaactcaaa ctcgactgaa
    11461 aagttatttt aaaaaagact aaaaatatcc cttctggact tgttcctctt attctgttat
    11521 actagagaaa cacgaaagat atttttatgc gacacttttc atttgttttt atgaaattta
    11581 ttcaaaactg gcgtaacttc agagttacaa ggatgtatta gaggttaggg aatacatcgt
    11641 ttaaatgaga aataagtaat agtcacccaa actttaaatt aatgaatatt gaatcgtcag
    11701 atagcctcct cgagttttcg agaaggctat cgtttatttt atggagaaat tgattcagaa
    11761 gtaatcagcg acctttttta gttcaccaat cgtttctgct tggaaagttt gcacccaatc
    11821 ttcttggaag cccctcactg ctttatcaat cgagggcatc tctaatactt gatctagtag
    11881 ctctttacct aacgttatcg catgagctcc agatcgaatg gctgtagtga cttgttcaca
    11941 atttttgaaa ctagctgcta atatttttgt tggtctttgc gtacgttcga tctcagtccg
    12001 caacgaatca atcactttca tagcatcaat gccttgattt tgcatcctgt tataataagg
    12061 tgcaatataa tctgctccat tattaatagc taaataccct tgtaattctg tgtaaatagc
    12121 tgtaccggta atcaaataac cttctttttt caactttttg atcgcttgaa ggcctgcttg
    12181 attcaccggc accttgataa acgtatcttt tcccagttct tctatgataa ctcgagcatc
    12241 ctctatcatt tcttcttccg ttcttcctat tgtctgaata tggataggaa tctgtccgat
    12301 tatttttttg atttctttca tatgcgagaa gaagtttgac gctccttctt ttttcagaat
    12361 tgtaggattt gttgttacgc ctgttaaatt caaaaactgt tgacaagaat aaatactttt
    12421 tgtatttgct gtatcaagca tgatttccat atagctttcc cccattctga tcctcttgat
    12481 cgattttaat gattgtcccc tttcttaaat gcggcatttc cttatcttct acatacattg
    12541 ttcctggaag ttccggctca tgagatccat caaattttat cgtgatatgt cctaattcat
    12601 tcaaattagt ttggacaaca tctcctactg ctgtgattac atacatttgt ttatcaaaac
    12661 agagcttcat atcacaagtt atttcttttc ttacctgatt caattcaatc ttataacaat
    12721 aatctgttaa tgtttccggt gcttcatctc caaataaaat cattattttt tctttcctga
    12781 atacttctgc ttctatacca acatctgtta caaccgtttc aaagatcgtc atctctgttc
    12841 ctcctcgtaa aatatcattt gtctaatagt ttttattcaa aatgtttcta gattataaaa
    12901 tttattgaat aacacattcc taacggaatt tgtttcgata ttgtgtgatt tctcagagta
    12961 caatggatgt atgga
//
LOCUS       DAJSBN010000072        12734 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJSBN010000072 DAJSBN010000000
VERSION     DAJSBN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.073"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ONT86_002434"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ONT86_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR2353189.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ONT86_002435"
     CDS             complement(322..891)
                     /locus_tag="ONT86_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCR2353190.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ONT86_002436"
     CDS             1077..1979
                     /locus_tag="ONT86_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2353191.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ONT86_002437"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ONT86_002437"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR2353192.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ONT86_002438"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ONT86_002438"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR2353193.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ONT86_002439"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ONT86_002439"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR2353194.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ONT86_002440"
     CDS             8547..8732
                     /locus_tag="ONT86_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCR2353195.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ONT86_002441"
     CDS             complement(8794..9990)
                     /locus_tag="ONT86_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR2353196.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ONT86_002442"
     CDS             complement(10160..11596)
                     /locus_tag="ONT86_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR2353197.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ONT86_002443"
     CDS             11889..12698
                     /locus_tag="ONT86_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2353198.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2675 c         2249 g         4007 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaacgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAJSBN010000073        11234 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJSBN010000073 DAJSBN010000000
VERSION     DAJSBN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.074"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            196..1455
                     /gene="lysA"
                     /locus_tag="ONT86_002444"
     CDS             196..1455
                     /gene="lysA"
                     /locus_tag="ONT86_002444"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR2353199.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1442..2242
                     /gene="dapF"
                     /locus_tag="ONT86_002445"
     CDS             1442..2242
                     /gene="dapF"
                     /locus_tag="ONT86_002445"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR2353200.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2245..3126
                     /locus_tag="ONT86_002446"
     CDS             2245..3126
                     /locus_tag="ONT86_002446"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR2353201.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3123..3839
                     /locus_tag="ONT86_002447"
     CDS             3123..3839
                     /locus_tag="ONT86_002447"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR2353202.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3845..4540
                     /gene="dapD"
                     /locus_tag="ONT86_002448"
     CDS             3845..4540
                     /gene="dapD"
                     /locus_tag="ONT86_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCR2353203.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4556..5557
                     /locus_tag="ONT86_002449"
     CDS             4556..5557
                     /locus_tag="ONT86_002449"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR2353204.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5554..6915
                     /locus_tag="ONT86_002450"
     CDS             5554..6915
                     /locus_tag="ONT86_002450"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCR2353205.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6875..8026
                     /locus_tag="ONT86_002451"
     CDS             6875..8026
                     /locus_tag="ONT86_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCR2353206.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8001..9170
                     /locus_tag="ONT86_002452"
     CDS             8001..9170
                     /locus_tag="ONT86_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2353207.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9345..10724
                     /locus_tag="ONT86_002453"
     CDS             9345..10724
                     /locus_tag="ONT86_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2353208.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11005..11234
                     /locus_tag="ONT86_002454"
                     /pseudo
     CDS             11005..11234
                     /locus_tag="ONT86_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3739 a         1956 c         2505 g         3034 t
ORIGIN      
        1 tttatgtata aatagcttct aatagttcgg gtattttgtt tttataacca tttttattct
       61 tatttttaaa aaaatatttc gaaatatact agacaattca gaaaattttg tttaatatta
      121 ttccaataaa attcaaatct ttatcaaatg aaattccaat aaaaacggaa aaaaagtgaa
      181 aagaggaatg aaaaaatgac agaattgata attggcggag tcacagcaag cacattggca
      241 gaaaagtata agacacctct gtatgtgtat gatgaagaaa agatgagaca aacgatgctg
      301 tcattcaagc gaggctttgt atcgaaacgt tttgatacaa aagtgcttta cgcctctaaa
      361 gcattccaaa cgattgagat gctgcattta gttcgtgaat atggattcgg gctagatgtt
      421 gtcagtggtg gggaaatcta taccgctttg caagctgatt ttcctagaga tactatctac
      481 ttccatggta acaacaagac gccagaggaa ctgatctatg cacttgaaaa tgatttgttg
      541 catattgttg ccgataattt gatggaagtt gaattattag cagaattaag ccaacgctat
      601 ttaaaaaaaa tctgtatcat gcttcggttg aatgtcggag tagaagcaca tactcatgaa
      661 tatatcgtca ctgcgcatat tgattcaaaa ttcggcatga gctatgaaag taaagagtgc
      721 caacaagccc ttcagttgat aaaagaaagc agctatctag agttagaagg atttcatgct
      781 catatcgggt cacaaatttt tgatgttact gcttggttag ctgaaatcga caaattgacg
      841 aattacttaa ccgattttga ccaacctctt tccttgaatc ttggcggagg gtttgggatt
      901 cgttatacac aaaatgatca gccgatccca gtcgaagagg cactaaaaaa actgattgaa
      961 tatacggaag aagctttgga aaaacgaaaa ctgaccattc gtcaattact gatcgaaccg
     1021 ggtagaagta ttgttggaga agctggtaca acactataca cgattggttt catcaaaaag
     1081 acaccacata aaaaatatta ctttgtagat ggcgggatga cagataatat ccgtccagct
     1141 ctgtatcagg cagaatacga ttgtgattta gcagcaaaac tgtcggaaga aaaaacagag
     1201 aaagtgacga ttgcaggaaa aatgtgtgaa tcaggcgatg tggtaattaa agaagcttat
     1261 ttgccgaaag cagaatcggg tgatctttta gctgtttatt ccacgggagc ttatggatac
     1321 tcgatgagca gcaactacaa tcgagcgaca cgaccagcag ttgtttttgt caataatggc
     1381 caatcaagac tgattgttcg cagacaagca ttttctgatc tactaagggg ggaagtttcc
     1441 tatgatcctt cataaatata atggctgcgg aaatgatttt atcttattag attacgcaga
     1501 agacacagat tatagtcaat tagccgcttc tttatgcaca aaagaaaaat atgatacaga
     1561 cggattgatc gccgtaaaaa tcgatccgtt ggaaatgatc tattacaaca aagatggcag
     1621 ccgcgcgccg atgtgcggaa atggcattcg ctgttttgct cgatacgtaa atgatcagaa
     1681 catgatgaat gaacaggaat tcaacgtaga gacattagca ggcacaagaa aaattacggt
     1741 gttggatccc tcaccttttt attgcagcgt agatatggga gtgccagatg attctccgga
     1801 aaaaatcaaa gctaatcaaa cttatccaat aaaaaaccag gaaatagaga tcaatggtga
     1861 aaaagtaacg atcactagtt tatttttagg aacgattcat acagttgttt ttgttgaaaa
     1921 tgcgcgtgag gaagtctgga gaaaaagagg tgaactgatc tgtcatcatc ctttgttcaa
     1981 agaaaaaacc aatgtcaact ttgtagaagt agtcaatgaa caagaattga tcgtccgtac
     2041 ctatgaaaga ggagtaggtt ggacacttgc ttgcggtaca ggttgctgtg cctcttatgt
     2101 ggttgctaag gaaactggga aaatttcagc agatcaggta gttgttcact tggaacaagg
     2161 agacttgatg atctctggtg aggatgcgat acaaatggct ggcccagcag ttcatgaatg
     2221 gacaagaata ttggaggaat gaagatgata aaaggttcga tcgtggcgct gatcacgccg
     2281 atgaatgaag agggatcagt agattatgct ggtttggaaa aattgatcca attccattta
     2341 gatgaacaaa cagatggatt attagtttta ggtacaacag gagaatcttc tacgctcaca
     2401 caatctgagg aggagcagat ccttcaactg acagtaaaga aagtggctgg ccgtgtgccg
     2461 gtgattgcag gagctggaac gaataatacg aaagaaacga ttgaaaaagc gaagcatttt
     2521 gcttctttgg gggcagacgc acttttggtc atcacccctt actacaacaa aacaagtgat
     2581 gcaggactag ctgctcattt tacagcaatt gcagaagcct caccgattcc gcttattttg
     2641 tataatgttc cttctagaac aggcatgtcg attccaatcc atgtattagt aaatttagca
     2701 gaacatccta atatcattgg tttgaaagaa gcctcaggag atatggctta tgtgatggat
     2761 gccgctcggt tgattggaga agaattcttc ctttacagtg gaaatgatga tttgatccta
     2821 ccagtaatga gtgtaggagg aagcggtgta ataagtgtat gggcgaatat ccaacctaaa
     2881 atagtccatg aattagtaaa agatacgcag gacggaagat ggcagcaagc aaaagaaaag
     2941 caattaaatg cacttgaact gatccatgcc ttgttcagtg agaccaatcc tattcctgtc
     3001 aaagcagcca tgtcattatt agacttacct tccggacctc tacgcctgcc tttagtttct
     3061 ctttcaaaag agaaaaagaa acaattagct cagcttttgt taaaagaaag gacggttgaa
     3121 aaatgaaaat cggactgatt ggcagtggac agatgggaaa tagagtcgaa gaagttgcac
     3181 aagaaagagg agatacggtc cttcttttac ggacggcacc taaagaagca actacgcttg
     3241 cttttacgga actgccagaa ctgcttattg atttttctca tccagataat ttagagaaaa
     3301 tcctttcgta tagcttatca tatcaacttc cgttagtcat tggcacgaca ggttatacac
     3361 aatcacagtt ccaagaaata aaagaacatg ccaaacatgt acctgtgatg tatagtgcta
     3421 atttttcgtt cggcatcatg gtgatgaatc aactaatcaa gcaagcagct gcaatgttgc
     3481 aaggctggca gatcgaactg attgaaaaac atcatagtcg taaaaaagat gcaccatctg
     3541 gtactgcaaa tatgctgctt caaacaatcc aagaagtgca aaagctgcag cccgtataca
     3601 actggcaagg gaaaaaaaga gaagaaaacc aaattggtgt acacagtatt cgtgcaggtt
     3661 ctttacctgg tgaacatgat gtattgtttg ctgctactga tgaaatattt acgatcaaac
     3721 atgaatcttt ttctaatcga atctttgctg caggcgcagt agaagcagcg gactggttga
     3781 aagatcagtc accaggttat tacaaattag aagatttatt aatggacaaa ggggtgtaaa
     3841 aaacgtggaa gctcaagaaa tcatccgcta cattcaaacg gcatctaaaa agacgcctgt
     3901 gaaagtctat ctgaatgtaa ccgagccaat cgaatttaaa gaaagtaaag tttttggtga
     3961 aggaagcagc ttcgttgttt ttggtgacta tgagacgatt gctccggttt tagaagctga
     4021 atcagaaaaa atcattgaaa tcgaaatcga aactgctgca agatacagcg cagttccttt
     4081 gctagatatt aaaaaaatta atgctcgtat cgaaccaggt gctattatca gagatcaagt
     4141 gtcaatcggc aacaatgccg tgatcatgat gggagcgatt atcaatattg gtgcagtaat
     4201 cggtgaaaac acgatgattg atatgggtgc tgtcttaggt ggacgagcga cagtgggcaa
     4261 aaactgccat attggtgcag gagctgtctt agcaggtgtg atcgaaccag catcggccaa
     4321 accggttatc gtagaagacg gcgtactggt aggtgcgaat gcagtgattg ttgaaggtgt
     4381 ccatatcgga aaagatgcag tcgtggcagc aggagcagtg gttttagaag atgtggcagc
     4441 agaaactgtt gtcggtggaa tacctgcacg tgtattaaaa ataagtgatg atcagacaaa
     4501 agaaaataca gcattgattg ctgcgttacg cgaattatag aggagatgga gaaaaatgcg
     4561 cgaaatagat acctgtattg tgggggcaac cggattagtc ggccaaacga tgctcaaagt
     4621 gttagaagaa tatgattttc cagtaggacg attgaaatta ttagcttcaa aacgttcagc
     4681 cggtaaagtt tgtgtattca agaaaaagaa acacactgta caggaattga cagaaacttc
     4741 atttgaagga tatgatttag cgctattttc tgcaggtgct tctgtatcca aagagtttgc
     4801 gccaatcgca cgaagtcagg gactatttgt catagataat tcctcagcat ggcgggaaga
     4861 ttcggatatt ccattaatcg ttccagaggt caatctggaa gactatacat tgaatcggct
     4921 gattgcaaat ccaaattgtt caaccattca agcggtactt ccgcttaaag ctttacaaga
     4981 agcctttggt atccagcgag tcaattattc gacctatcaa gcagtttctg gttccgggca
     5041 aaaaggcatt gaagatcttc atcgtacgca aaatgacgag gaaccagttt tgtatcctta
     5101 taatattagc caaacggtga ttccggaaat cgatcagcct ttggaaaatg ggtatacaaa
     5161 agaagaacag aaaatgataa atgagacccg taagattctg catcagcctg ctttgccagt
     5221 atcagcaacc tgcgtacgtg tgccaatcga aaatggacat ggagtctctg tggcagttca
     5281 actgcaaaaa gagtttacga cggaagaaat tagagagtgt ctcagccagt ttccagggat
     5341 tcaattagaa gatgatctag cagcacataa atatccgacg agcattcttg ctagaggaac
     5401 agatatggtt tatgttggga ggatcagaaa agacacttct ttagagaacg gattgttgtt
     5461 ctatacgact gccgataaca tacgcaaagg agccgctgcg aatgccgtac agattgctgc
     5521 agctttaatg cagaaaatag cggaggtggc aagatgagta atacgctgaa agtagcgaaa
     5581 ttcggcggaa catcgatgtc tcatgaagag caattcaaaa aagtccgtac aattgttctt
     5641 tcagatccca atcgccaagt gatcgttgtt tctgctttag gcaaacgtac gtcagatgat
     5701 gacaaagtaa cagacttact ttatttgatt tatgcttacc ttcagcatca tgttgattgg
     5761 acaccccttt ggaaaaggat ttgtgatcgt ttcattcaag taagagatgt tcttgccctg
     5821 cagttaccta ttgaagatga actagcacgc atacagaaac aactggaaga cgaaaagata
     5881 actgaagatt atttagtaag tagaggtgaa tatctgactg ctcaactgat ggctgagtat
     5941 cttggttacc agtttattga cgcaaaagac ctgattttct ttgactatga tgggaaaatc
     6001 gatcaggaaa aaacagcaca ggaaatcaca aaagcattta aaagatatcc gaaaatggtt
     6061 gttccagggt tttacggggc gaaccctgca ggaaaagaaa aattattggg ccgtggggga
     6121 agcgacatta ccggcgcgat actggcgaag atgttgcaag cggaaagtta tgagaattgg
     6181 acggatgttt caggaatcat gatggctgat ccgcgaatca tcgatcatcc taaaatcatc
     6241 gaggaaatct cttttagaga actgagagag atggcttata tgggggctaa cgttcttcat
     6301 gaagcagctg ttttcccagt tcaagaggct gatatcccca tccaaataaa aaataccaat
     6361 gatcctttcg ctcaagggac aaaaatatcg aatcacgaaa tagaaaagga aaatggactg
     6421 acaggaatag ctggacgcaa ggattttctt tccatcactt tattcaaacg tcatatgtcc
     6481 gatgaaatcg ggtttatctg gaaagcaatg agcatttttg ctaaacatga gatatcaatc
     6541 gaacatattc cttcagggat cgacaatatc ggagtgatcg tctcagcaga agcgattgcc
     6601 gataagttat ttctcatcac gaaagaatta aaagaaacac ttggcgtgga agaaatcgtc
     6661 gtgatcgaag acttagcttt gatctctgtc gttgggggtc cgcataaaga attgattgga
     6721 ttatctggga aagtgttatc gatattgaac aaattagaaa ttcgcacatc gatcttatca
     6781 caaggagcac aggagttgaa tctgatcatc ggtgtaccga ataatcaata tgaaacagta
     6841 gtgaagggta tctatgaagg gatggtgaat acggatgctg aaagaatatc gaatggcact
     6901 ccatcaaatt cctgaaatcg ggtttcatga attcaagaca aaaaaatatt tatatgaaca
     6961 agtaaaaaac agcggggccg ttatccatga aatcggcgaa acaggactac tcctttattt
     7021 tgataatcag caagaagaaa cgattgcttt tcgaacagat atcgatgcac tgccagtcaa
     7081 agaagaaacg aatctctctt ttgcttctaa gcatgaaggt tatatgcatg catgtggtca
     7141 tgacggacat atggcgatgc ttttagggtt gactgacttt ataactaaac acattgagga
     7201 tcttatttat aatgtagtac tgatctatca gccatcagaa gaaatttccg gtggcgcaca
     7261 aagcgttatt cagtcaggat tgttggataa atatgaagta caagcaatat ttggctttca
     7321 tctatggcct ggactagcta agggagaagt tttctctcga ccaggacctt tgatggcaca
     7381 aagcagtgaa acagacatta tcattaaagg ccagtctgca catattgctt ctagtgaaaa
     7441 aggaatcgat agtctggagg cagctgtacg atttttgaag aatgtttatg attttgaccg
     7501 gcaattaccg gcagagtgga atcatctaat gaaatttggc caaatccaag gtggaacgat
     7561 ccgcaatgtt ttagctggag aagttattgt atccggaagt atccgttctt acagttcaga
     7621 tatgcaaaat tatttgaaga ggggattaga ccatttagca gaacagtttc actctacagc
     7681 tactatttct tttcgctata atgacggcta tccagctgta atcaaccata gaggcttata
     7741 tgcagcactg ttagattcaa acaaactaca tgaattagat gctccagttc ttcaagccga
     7801 agattttggc atatatacgc aacaatatcc atgtgtattt ttctttttag gtgtcggtga
     7861 tacccctgct ttacatgatc cgacgttcga ttttgatatg gatgttttag aaaatggtgt
     7921 agaatggtac aaaacgattc tttttacaga aaatcttctt gtaaaaatcg atagcagttc
     7981 aaaagaggag cgagtaaata atgatgaatt tatccaatcg atttagtcct aggctttcta
     8041 gaatagaagt atcaaaaata cgacaattcg atcagcaaat atcgtcgata ccagatgtga
     8101 tcaaactcac cctgggagaa ccggattttc caactcctga acatgtcaag caagcaggaa
     8161 tagcagcgat tgaagaagac ttttctcact ataccggaat gagaggatta gaggaactgc
     8221 gggaagcggc ttgtatattt cagcaacagc gttatggatt aacttatgat ccgcaaacgg
     8281 aagttttgac gacagttggt gcaacggaag cgattgcctc tgcgttgtta tctgttttgg
     8341 aagaaggaga caaggttctt attccagcgc ctgcttattc aggatatcag ccattagtgg
     8401 aactagctgg tgctgaattg atcccgatag atacttctga cacagggttt gtctgtcagc
     8461 cggaacaatt tgaacgagca tttgaacaat atggatcagc tgtcaaagca gtcattttaa
     8521 actatcctaa caatcctact gggacgacat tgtctgcaaa acaattgaaa gcaatcgctg
     8581 aggttttaaa aaaatatccg gtatttgtta taagtgatga agtatatgct gaattgactt
     8641 atagtggaac gcatatgtcc atagctactt atcttccaga acaaacaatc gtgataagcg
     8701 ggttatccaa gtcacatgcg atgactggtt ggcgagtggg ctttattttt gctcaaaaag
     8761 cccttattga cgaactaatt aaagtccatc aatatctggt gactgccgct acaacgatgt
     8821 cgcaaaaagc tgcggctgaa gcgctaataa atggggtgaa agatagcgag aatatgaaag
     8881 agcaatatca aacacgaaga gactatctgg tccagcaatt ggcgccttta ggatttgaag
     8941 tgacccagcc aaacggtgca ttttacttgt tttgcaaact cccagctgcc attcaaactg
     9001 actcttggca gttttgtgta gaattagcgg aacaggcaaa agttgcgtgt atcccaggaa
     9061 ttgcatttgg cccagaagga gaaggttata tccggatttc ttatgctagc agcatggaaa
     9121 aattacatga ggcctgcaaa cggatcaaag actttttaac agaacagtga ttcatagatt
     9181 ttatcaaaaa aacaatcgtt aattttgcgg tatattctgc aaggagacga ttgtttttta
     9241 aatttagttc aaatgaacgc gtttcttttg attcataact aatgtaatgt taagtttaaa
     9301 ttgtttagaa tgattttaaa agaatattca aggaggtttc atcaatggaa aaaatcaaag
     9361 ttgtcattgt aggtgcatct catggagggc atcaatcgat cttggaacta ttgacacgat
     9421 acggtgaaaa tgtagatatc acgctatttg aagcaggaga ctatatttcc ttcatgtcat
     9481 gtggtatgga gctgtattta gaagacaagg taacagatgt aaatgatgta cgtaatttca
     9541 gaccagaaaa ctttccgcaa tccaatgttc atattttaaa taatcacgaa gtaaaaacga
     9601 tcgatgccga taaaaaaaca gtaacggtta ctagcgcatc agataacgaa acaaaagaat
     9661 atccttatga caaattgatt ttgagttctg gtgtcaaacc aaacaatctg ccagtaccag
     9721 gagcagatct agaaaatgtc tacttgatgc gtggatacaa ttgggcaacc aaaatcaaag
     9781 aaaaattaaa tgatccggct attaaaaaaa tcgctgtcgt aggtgcgggt tacattggga
     9841 tcgaagcagc agaagcaagc cgaaaagctg ggaaagaagt cgtattgctt gatgtgatcg
     9901 atagaccttt aggcacttat ttagatgctg agatgacaga tattttagaa caacacctaa
     9961 atgaaaacgg tattgaagtt ttgacaaatg caaaaatcga agcttttact ggtaacggaa
    10021 aagttgaggc aatcaaaaca agtgaaaaag aaatcccagc agatctagtc attcaagctg
    10081 ccggtgtgaa ggcaaataca gaatggttaa aagggatcgt cgacttagat gaacgtggct
    10141 ggatccaaac aaatgaatac ttgcaaacga atcttccaga tgtgtatgca gtaggagatg
    10201 ctacacttgc ttattctatc ccagcacgca aaaaaatgcc gatcgcttta gcgactgttg
    10261 cacgtagaga agcacgttat gtagtaaaac atctttttga agaagtacca aacaaaccgt
    10321 tcggcggagt cgtaggttcc tctgcattaa gtgtctttga ctatcatttt gcagcaagcg
    10381 gattgaatag ctttacagca gcaaaagcag gagtagatgt acgagtgtct tattatgaag
    10441 atacgttacg accaaaatat gtaccagaag aacaaggaaa tccaaaagtg tccatccaat
    10501 tattctcgga tccaatgacc catcaattgc taggaggagc agtattatct acctatgatg
    10561 ttacaggtca aggaaatgta ttggcactag cgatccaaaa caaactgact ttagaagaac
    10621 tagcagaagc tgacttcttc ttccagccag gctttgaccg acaatggagc atgttgaatc
    10681 tagccgctca acaagcgtta ggagaagaac catttgtaga ataagaaaac gaaatatttg
    10741 aaaagaggtt gtgaaaaagc tgccttgcat caagtaataa gaacagccaa gtaaaaacag
    10801 cttcttatgt ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt
    10861 ttattttcaa aaaagggact gtgacacgat ttgtgtaccg acccccaaaa gttagaccaa
    10921 aaaatctaac ttttggaggt cggtattttt tatggttaaa tatacttttg aacttaaatt
    10981 aaagatcgtt catgattatt tggatggcaa aggcgattgg accgtaaaaa tccagatcaa
    11041 gagattgaag cggaaatgct taaaattcgg gagaagcaca aggattatgg ctgtttacgg
    11101 atgaccaatg aactaagaaa ccgcggtttc tctatcaata aaaagaaagt tcagcgcttg
    11161 atcaagaagc taggaataaa agtaacggca tacacgcgta aatcacgtcg ctacaattct
    11221 tatcgaggaa aagt
//
LOCUS       DAJSBN010000074        11031 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJSBN010000074 DAJSBN010000000
VERSION     DAJSBN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.075"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            131..505
                     /locus_tag="ONT86_002455"
     CDS             131..505
                     /locus_tag="ONT86_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2353209.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ONT86_002456"
     CDS             complement(619..1248)
                     /locus_tag="ONT86_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2353210.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ONT86_002457"
     tRNA            complement(1382..1454)
                     /locus_tag="ONT86_002457"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ONT86_002458"
     tRNA            1668..1741
                     /locus_tag="ONT86_002458"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ONT86_002459"
     CDS             complement(1879..2043)
                     /locus_tag="ONT86_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353211.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ONT86_002460"
     CDS             2338..3666
                     /locus_tag="ONT86_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCR2353212.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ONT86_002461"
     CDS             3868..4701
                     /locus_tag="ONT86_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR2353213.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="ONT86_002462"
     CDS             4717..5454
                     /locus_tag="ONT86_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCR2353214.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6535)
                     /locus_tag="ONT86_002463"
     CDS             complement(5609..6535)
                     /locus_tag="ONT86_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR2353215.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6810..7643
                     /locus_tag="ONT86_002464"
     CDS             6810..7643
                     /locus_tag="ONT86_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR2353216.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7677..8516
                     /locus_tag="ONT86_002465"
     CDS             7677..8516
                     /locus_tag="ONT86_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2353217.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8542..9102
                     /locus_tag="ONT86_002466"
     CDS             8542..9102
                     /locus_tag="ONT86_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2353218.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9301..11022
                     /locus_tag="ONT86_002467"
     CDS             9301..11022
                     /locus_tag="ONT86_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCR2353219.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
BASE COUNT         3762 a         1816 c         2215 g         3238 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt tttttcattt tacagacttc cttacgaata
     6601 attttgcata gccgttaaat cttattgtat actatataaa agagttttgc tatcagtgaa
     6661 gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg gaaaagactc
     6721 aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa gaaagcagcc
     6781 aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca tcgtttaaag
     6841 aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca gggtttctac
     6901 ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca gttgagaatt
     6961 ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg
     7021 gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg cattttttag
     7081 atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc caattcccct
     7141 catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga aagtggtttg
     7201 agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat gaatttcttg
     7261 aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc gatgagccac
     7321 agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac tttgctcttt
     7381 ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa
     7441 aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat gaagtcaaag
     7501 tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct ggtggtgatg
     7561 cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat ggactcaatc
     7621 caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg aagtctatga
     7681 tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca aaagcgttgg
     7741 aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa
     7801 cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta gatatgtatg
     7861 tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac cacccttttg
     7921 aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga caaatcgttt
     7981 ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa tcgattttta
     8041 tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc
     8101 tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag atattaaaag
     8161 aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag ttagaacatc
     8221 gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc atcccaaaag
     8281 gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag
     8341 aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt gtaggaattg
     8401 gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt gtgactcaat
     8461 caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt tattagtcaa
     8521 tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt agaaacattc
     8581 cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga aaatgaagct
     8641 catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc tacttcacct
     8701 aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga taaagcagaa
     8761 gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca agtagatgca
     8821 ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat agtgcctgat
     8881 cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga tggaaaacct
     8941 cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat ggaatatgct
     9001 ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat tgctaagaag
     9061 aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt tcactatttt
     9121 tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga atgcatgaaa
     9181 atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta tttggattta
     9241 tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga ggaaccataa
     9301 atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc
     9361 tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa
     9421 cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt taaaaaaatg
     9481 catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg gaatgacata
     9541 tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa cctcaatgaa
     9601 agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac aatggaccaa
     9661 atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga aacggaagaa
     9721 cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa agaaaaagtc
     9781 agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg
     9841 atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc tgttgaagcc
     9901 cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct gatgaaaaaa
     9961 attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga agaaatcagt
    10021 gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa
    10081 gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga aaaaacagaa
    10141 gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc gttatatgat
    10201 atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc aacgattgca
    10261 gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct ggatcacaca
    10321 tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa
    10381 gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca acacgaaatt
    10441 ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga agatatcgaa
    10501 aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga aaaagaagcg
    10561 caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa
    10621 caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc tactgaccgt
    10681 attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga ggtcaatcgt
    10741 ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca tgatttagta
    10801 gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg tcatacacac
    10861 gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga atatcactat
    10921 caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg tgctttcaaa
    10981 cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa a
//
LOCUS       DAJSBN010000075        10928 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJSBN010000075 DAJSBN010000000
VERSION     DAJSBN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.076"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..287)
                     /locus_tag="ONT86_002468"
     CDS             complement(<1..287)
                     /locus_tag="ONT86_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2353220.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAE"
     gene            429..1547
                     /locus_tag="ONT86_002469"
     CDS             429..1547
                     /locus_tag="ONT86_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2353221.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1675..3051
                     /locus_tag="ONT86_002470"
     CDS             1675..3051
                     /locus_tag="ONT86_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR2353222.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3452..5032
                     /locus_tag="ONT86_002471"
     CDS             3452..5032
                     /locus_tag="ONT86_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR2353223.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5230..5453
                     /locus_tag="ONT86_002472"
                     /pseudo
     CDS             5230..5453
                     /locus_tag="ONT86_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5658..5978)
                     /locus_tag="ONT86_002473"
     CDS             complement(5658..5978)
                     /locus_tag="ONT86_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR2353224.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6126..8360)
                     /locus_tag="ONT86_002474"
     CDS             complement(6126..8360)
                     /locus_tag="ONT86_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2353225.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8839..9105
                     /locus_tag="ONT86_002475"
     CDS             8839..9105
                     /locus_tag="ONT86_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCR2353226.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9105..10832
                     /gene="ptsP"
                     /locus_tag="ONT86_002476"
     CDS             9105..10832
                     /gene="ptsP"
                     /locus_tag="ONT86_002476"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR2353227.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1912 c         2215 g         3322 t
ORIGIN      
        1 ttttctgctt cttcaatcaa tatttttcct aacccttggt gtctgtattc agaaagaatc
       61 gccatccgct gtaatttcat ttttccgttt tctaaaggca aaagacgtac agttccttgc
      121 ggttcttttt tatctgagta caggacgaag tggatacaat gggcttcatc tttgtctatt
      181 tctcgagaca aaggcacacc ctgttcgact acaaaaactt gatgcctgat ccgtacagca
      241 tctaagtaaa tattgctcat tgtatctttt gtttgtacaa ctttcatggt ctagccacct
      301 tcctttcgta tagtatactg gaaaataaaa caaataaaaa gtatggcaag ttttcaaaaa
      361 taaaaaaaga ataaaagaat ttttaacgaa tataatatat actagtaaga aagaaaggag
      421 gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg cttgaattat
      481 tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc gaaccgatcg
      541 ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt tactatttat
      601 tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg gctgtcgcaa
      661 ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc atcccaagtc
      721 tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa caggtagaaa
      781 gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc aataaatatt
      841 ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt ggagtatcca
      901 atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc attacagcac
      961 cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac atccaacagt
     1021 ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg aataaaacat
     1081 tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc ttcacatttt
     1141 taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt gcgggattga
     1201 caaacctaat tccatattta ggaccttatc taggactagc acctgcattt ctagtaacag
     1261 tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc gtccaacagt
     1321 tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc catccattaa
     1381 cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt atcttcttag
     1441 gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa atcgtcaaag
     1501 aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta gttttagtac
     1561 acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg ctatagtgcc
     1621 acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc gattatgaat
     1681 gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt tttgacattg
     1741 atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa gaatcgaatc
     1801 gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat attgcagaat
     1861 ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat tttgtctggt
     1921 gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg cggcacagcg
     1981 gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc gattgttttt
     2041 ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc aaagttcact
     2101 tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct gctttctgct
     2161 tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga ttcaaaaatg
     2221 accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa tgaagaatta
     2281 gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt catggtccca
     2341 cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt gaatgaagtt
     2401 ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa agtagtcgga
     2461 atccttcata caaagaattt actaaaagct gcccataaat tcggctttga taatttagat
     2521 atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat tgatgatctg
     2581 ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga atatggcggc
     2641 gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga aatcgatgat
     2701 gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata tatcatccaa
     2761 ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca tatgagtgat
     2821 gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga tgaaggcgaa
     2881 aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga aggttctcga
     2941 atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga acctgaagaa
     3001 gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata attggaaact
     3061 tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt ttagtcagct
     3121 gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga atccatgcta
     3181 tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc attaagtaat
     3241 aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct tattgctgat
     3301 aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc ttttgtcaca
     3361 gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag agaaaatata
     3421 aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa agcaacaagt
     3481 ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa caacgattac
     3541 agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa aaggcaaaaa
     3601 aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg ggatttctgt
     3661 tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct tggatacacc
     3721 aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg atagtgcggt
     3781 catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat tccaagttgt
     3841 aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg atggtcgtga
     3901 accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt atccgatgaa
     3961 ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc ataatcgtgt
     4021 agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg atgaagatgg
     4081 actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag tcttagaaga
     4141 agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg ctcgtggcga
     4201 tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga cttttttaga
     4261 gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg atcagatggt
     4321 aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa atatgaatcc
     4381 agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg aacgaggaat
     4441 ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca cacaatttat
     4501 ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg gtgtatatga
     4561 tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag ttgcttatga
     4621 agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa atgtcatgaa
     4681 acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga ttcagcttta
     4741 taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac aattcgaagt
     4801 attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc caatgggaca
     4861 caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt caagtcgcaa
     4921 tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc aatttgcaga
     4981 acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt aaagaagtct
     5041 tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag cctctcatat
     5101 tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt aaaatcatag
     5161 aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa agaaaggaga
     5221 gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa aaagaagaga
     5281 tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag caaattgcac
     5341 aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac acgcgtagaa
     5401 ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc tagaaagaaa
     5461 aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac aaaaatttga
     5521 aaagcaattt tcgtgaattt cagcttattt ccgaaggagt tgcttctgct ttcgccgttt
     5581 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tctttaatta
     5641 ttatgttcaa aatttgttta cacggctaat gtagcgccta attcatgttt tggaatcgag
     5701 atttcaacaa ggcctgccaa ttcgattact ttgatttcat cctcgtggta aacggcgtct
     5761 ttgattttat ctaagaaata atttttagca tcttcgattt ctttcggctg gattttacgt
     5821 ttgtcattga tcagcagaat ttcattatgt tcaggggctt cagtgtagac aatagtggta
     5881 tttccggctg tgtagacctt gactagatgg gcatcagtgt tttcaagctg gtttaataca
     5941 agacgggaat ggctgtttgt tacatttacc aatttcatgg cgctcacctc ttttatgtat
     6001 ttttaattgc ttgtttctct tattataatt tcttttttat aaaatgaaca gtattttgac
     6061 ttttctgaaa aaagaatgag acggcatttc tcaaagaaat accgtctcac gctataaaac
     6121 tggatttatt cagccgtttc ttcggctgat tctttagcca aacgttctgg tttagaagta
     6181 acgatgattt ttccatcttt atccagtttt gctttcaact catggataga agggtgatca
     6241 agataaaatt cagcgatacc atcttcgatt tgttcttgga tcgtgcgtcg aagaggacga
     6301 gcacccatag atggatcata gcctaagtca actaattttt ctttgacatt cgtaggaact
     6361 tcgatgtgaa gttgctgtgc tgccagcatt tggttgacat cgtctagcat tagtgtgacg
     6421 attgtcatca agttttcttt tgataatgca ctgaattcga tgatcccgtc aaaacggttc
     6481 agaaattctg gtgtaaagta gttgtttaat tggttcaata cagaacgagt aacaccttcg
     6541 cgtgccgcac caaaaccaac gtttgcttct actttaccag ttccggcatt acttgtcata
     6601 atgatgatcg tatctttaaa gctgaccgtt cttccttgtg catctgtcaa tcgaccatca
     6661 tctaagattt gcaagaacat atgcaaaaca tctggatgag ctttttctac ttcatctaac
     6721 aagacaagac tgtaaggatt tctccgtact ttttctgtta attgaccagc ttcttcgtat
     6781 ccgacataac ctggaggaga accaatcaat ttagagacac tatgtttttc catatattca
     6841 gacatatcga aacgaatcat tgaatcttgt gaaccgaata gttcgtaggc aagttgtttc
     6901 gcaagctctg ttttacctac accagttggt ccaacaaata agaaggagcc gattggacga
     6961 ttctgtttgt ttaatccaac acggtttcgg cgaatcgctt tcgctactcg atctacagcg
     7021 ttatcttgtc cgatgacatg agctttcaag tcatctgcta agtttttcag ttgtgtctgt
     7081 tctttttctt ttaattcacc cactggaata cctgtccgtt gttcaacgat tttttccata
     7141 tctttttcgg taatcacagg tgtttcctct tcagtgagct gacgttcttt cattttttga
     7201 agtttattga tctgatcgcg ataataagct gctttttcga aatcttcttc tcgagaagct
     7261 aaaacttttt gttcttctgc ttcttgcagt tttttctcga ttgttttagg atcaacgatt
     7321 tggatcgtca agtttttctt agaacctgtt tcatctagca aatcgatggc tttatccggc
     7381 aagaagcgat cttggacgta acgattggat aaagttgcgg ctgcttcgat ggcctcatca
     7441 gtatatttta catgatgata gtcttcataa cgtttttgca aacctttcaa aatactgatc
     7501 gtttcttcga cagttggttc atctacccgt acaggttgca tacgacgttc taaagcagcg
     7561 tctttttcga tgatgcggta ctcatttaag gtagtagcac caaccatttg caattctcct
     7621 cgagctaaag ctggttttaa aatattacca gcatccatat ttccatcacc agcagaacca
     7681 gcaccgacga tttcgtgaac ttcatctatg aataggatca cattttctgc ttgacggatc
     7741 tcttcaatca atttttgcat acgttcttca aattgtcctc gtataccagt accttggact
     7801 aaagatacga catctaaacg aatgacttct ttgtccatta atttttgcgg tacatcacca
     7861 tctacgattt tttgtgctaa accttcaaca actgctgttt taccaacacc tggttctcca
     7921 atcaagacag gattgttctt ggttcggcga tttaaaattt caatgactcg tttgatttcg
     7981 tcatcgcgtc cgatcacagg atcgatatca ccttgtcgtg cttgttctgt gatattgatt
     8041 ccgtattctc ccaataaacc ttctgtattg ccttgaggtg gctgaccacc attgaagttg
     8101 ttgtttccgc caaattgtgt tggtggtgtt tgctcataat ttccttgctg ttgcatttgg
     8161 cgagacatgg aacggaaaag atcgtccaaa ctgccaaatc caaatggatc attttgggcc
     8221 atattagtca gacctccatt ttgttgattt ttgatttttt gataacagct ctgacagtaa
     8281 tcgagttgtg tccgctgacc attgacagtg gcatacaagt gaatcgttgc ctcattcttg
     8341 ccgcagtttt gacaaagcat gaatcattca cgtcctttca caaaaatggg tacatccata
     8401 ttttacatct cgtttttcat gaggtaaaag aataacccct agttttttaa gagatttatg
     8461 aggtagaaaa ggtgaaatct ctattgacca ttcctgacct ttgactctta ttatactaaa
     8521 aataaaaagg gaagcaagta attcgtttca tatgaaaaca aagagggtca aaggaaagaa
     8581 tgataaacgt caggagaaga gaaagcgaca agaaaaagga aataaacacg tttgttataa
     8641 aatgtgaaga aaaggatgaa aaagaatccg gaataatttg aatactttta tctgaaattg
     8701 aatgaaaact gttgtatcta acgaaaaaaa atgttaatct ttacagatga aagggtggca
     8761 tttgttagga ttctctgaat tactaaaaaa gaaaaccctc acataaaaac aacactcact
     8821 taaaggagac cgattaatat ggaaaagaaa gaatttcacg tagtagcaga aactgggatc
     8881 cacgctcgtc cagctacact attagtacaa acagcaagca aatttaactc tgatgtaaac
     8941 ttagagtata aaggtaaatc agtaaacttg aaatcaatca tgggcgttat gtctttaggt
     9001 gtaggtcaag gttctgatgt tactattact gcagaaggtg ctgacgaagc tgatgcaatg
     9061 gcagcaatcg ttgaaacaat gaagaaagaa ggcttatctg aataatggtt gaaatgctaa
     9121 aagggattgc cgctagtgac ggtgtagccg ttgcaaaagc ttacctgcta gttcaaccgg
     9181 atttaacatt cagtaaggca acagtagaag atactgcggc tgaagaagct cgtttggatg
     9241 ctgcattagc taaatctact gaagaattgc agcaaattcg tgaaaaagca gcgcaaagct
     9301 taggtgaagc agaagcgcaa gtatttgatg cccatttgat ggttctttca gatccagaaa
     9361 tggtaggtca aatcaagcaa aatattaaag acaacagcgt aaatgctgaa tcagcgctaa
     9421 aagaagtaac ggatatgtat atcggtatgt tcgaagcaat ggaagataat gcttatatgc
     9481 aagagcgtgc tgcagatatt cgtgacgttg ctaaacgtat tttagcgcac ttattaggcg
     9541 ttactttacc aaatccatca atgatcaacg aagaagtggt cgttgttgca catgacttga
     9601 ctccaagtga tacagcacaa ttagatcgca attttgtaaa agcgtttgta acagacattg
     9661 gtggacgtac ttcacattct gctatcatgg cacgttcttt agaaattcca gcaatcgttg
     9721 ggacaaaaga aattactgct aaagtcaaag aaggcgatat tttggctgtc aacgggatcg
     9781 aaggcgatgt aatcatcgat cctacagatg aacaaaaagc tgaatttgaa aaagcaggtg
     9841 cagattacgc agcacaaaaa gcagaatggg aaaaattaaa aaatgcagaa actgtcacag
     9901 ctgacggcaa gcattttgaa ttagctgcaa acatcggaac accaaaagat ctagttggtg
     9961 tccataataa tggtggagaa gcagttggtc tgtaccgtac tgaattcctg tatatggatt
    10021 ctccagactt cccaacagaa gatgaccaat atgaagctta caaagctgta ttagaaggta
    10081 tggaaggaaa acctgtagtt gttcgtacaa tggacatcgg tggagataaa gaactaccat
    10141 atctgcaatt acctcatgaa atgaatccat tcttaggata tcgtgcattg cgtatcagtt
    10201 tatctgaaca aggcgacgaa atgttccgta cacaaatgcg tgcattgctt cgtgcatctg
    10261 ttcatggtaa tttacgtatc atgttcccta tggtagctac actcaaagaa ttccgcgcag
    10321 caaaagcgat ctttgaagaa gaaaaacaaa aattgataag tgaaggcaag gaagtatctg
    10381 atacgatcca agtcggcatc atgatcgaga ttccagcagc agcagtctta gcagacaagt
    10441 ttgcaaaaga agtagatttc ttctctgtag gtacgaacga tttgattcaa tacacaatgg
    10501 cagcagaccg tatgaacgaa cgcgtttctt acttgtacca accatataac ccatcaattt
    10561 tacgtttgat caaaaatgta attgatgcag cacacgctga aggaaaatgg gcaggtatgt
    10621 gtggagaaat ggctggagat caaacagctg ttccattgct agtaggtatg ggtctagatg
    10681 agttctcaat gagtgcgaca tctattctta aaacgcgtag tttaatgaaa cgcttggaca
    10741 cagcgaaaat ggctgaactt gctgatcgtg cattgaaaga atgcgatatg atggaagaag
    10801 tcgttgaact tgttcatgaa tatgtaaaat aaacaacgta tcaaaagctg gtgtggcttg
    10861 taaaagagct acgccagttt ttttgttctt ttttaagaaa acgtttttgt cagtaagaat
    10921 agacaaaa
//
LOCUS       DAJSBN010000076        10817 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJSBN010000076 DAJSBN010000000
VERSION     DAJSBN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.077"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(25..162)
                     /locus_tag="ONT86_002477"
     CDS             complement(25..162)
                     /locus_tag="ONT86_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR2353228.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="ONT86_002478"
     CDS             complement(159..1343)
                     /locus_tag="ONT86_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2353229.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="ONT86_002479"
     CDS             complement(1599..1853)
                     /locus_tag="ONT86_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353230.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="ONT86_002480"
     CDS             complement(1878..3020)
                     /locus_tag="ONT86_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HCR2353231.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="ONT86_002481"
     CDS             complement(3080..4432)
                     /locus_tag="ONT86_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR2353232.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="ONT86_002482"
     CDS             complement(4503..4847)
                     /locus_tag="ONT86_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR2353233.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="ONT86_002483"
     CDS             complement(4857..5231)
                     /locus_tag="ONT86_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2353234.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="ONT86_002484"
     CDS             complement(5244..5558)
                     /locus_tag="ONT86_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2353235.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ONT86_002485"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ONT86_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="ONT86_002486"
     CDS             complement(8976..9638)
                     /locus_tag="ONT86_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353236.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="ONT86_002487"
     CDS             10255..10485
                     /locus_tag="ONT86_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353237.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="ONT86_002488"
     CDS             10491..10790
                     /locus_tag="ONT86_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353238.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         3000 a         2387 c         1833 g         3597 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgctt
//
LOCUS       DAJSBN010000077        10626 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJSBN010000077 DAJSBN010000000
VERSION     DAJSBN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.078"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(152..2305)
                     /locus_tag="ONT86_002489"
     CDS             complement(152..2305)
                     /locus_tag="ONT86_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCR2353239.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2599..3447)
                     /locus_tag="ONT86_002490"
     CDS             complement(2599..3447)
                     /locus_tag="ONT86_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2353240.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="ONT86_002491"
     CDS             complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="ONT86_002491"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR2353241.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5948..6127)
                     /locus_tag="ONT86_002492"
     CDS             complement(5948..6127)
                     /locus_tag="ONT86_002492"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCR2353242.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6174..7997)
                     /gene="cadA"
                     /locus_tag="ONT86_002493"
     CDS             complement(6174..7997)
                     /gene="cadA"
                     /locus_tag="ONT86_002493"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR2353243.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8275..10554
                     /locus_tag="ONT86_002494"
     CDS             8275..10554
                     /locus_tag="ONT86_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2353244.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         3050 a         2265 c         1897 g         3414 t
ORIGIN      
        1 caatttttgt cacaaccttt tttgccgagt aaacggtgtt caaaagccag tttctcggta
       61 tgaagtcaaa tttaagcaaa aatgtgagaa gctattttca caaaattttt cttcatactc
      121 gaaactaagc ggctttttca cgacctcttt tttattttgc catgaacata tcataatacg
      181 cgtccattat ttctttagca atcgtcaagt ttatgtggtc ttgttcgtct aataagttcg
      241 gcaagacaac actgatggca atttccggat tgtctgttgg tccgtatgcc acgatattac
      301 tattgactgt cgcaatatct ggatgacctt ccgcaaccgt ttctgcagtc cctgtttttg
      361 cggaaaggtc cattttcgca ctggctaatg gtttggctgt tgtgtaagga tcggtaccat
      421 ggacaacttg gtggaatcct tcacggagga tcgccatatt ttctgcagaa atattgaccg
      481 tattttctac tgttgtgcca atttctttct tcacttcacc caagtttcca ttttcgtcat
      541 tgccatagat cgctttgact aggtgcggct gtactcgttt cccgccatta gcgactgttg
      601 aagcatattg agctaattgg attggtgtat atgtgtcaaa ctgtccaaat gccaaatcgg
      661 taaagttacc tggagcatac cattctttgc cattatccgc attgaacttc ttcatataat
      721 caacagaacg tgagatccca ggagattcat tcggcaaatc gatcccagtc gttgtaccaa
      781 gaccaaactc tttgaaagag tctctcaatt gctgataagc tttagcttct tcatctaaac
      841 taggtagtcc cattccaggt gtatagtcta gtcctaacat ctttaacgcc actttgatca
      901 tatacgtatt agaagacaat tccgatgctt tgactgcatc aagagcaact tgtccgcttc
      961 gattaaagac agagcttttt tctgaagcac cttgcagacg aatcggctca tcaatcaaaa
     1021 cttgatttcc gctgatcgcg ttacttgccc agcctgctgt caaagtacca gccttgacta
     1081 cagaccctgg atcaaaggcg ctagtaatcg tgcctaaagc attctcggtg atttctttgg
     1141 aaccttgttc atgactgaaa ccagtcattg ctagcacttc tcctgtctga ggattcatag
     1201 ccactgcata agcaccaggc gaatactgtg ctttgccgtt gttgatcaat gtttgataat
     1261 tccttttcaa aatttcttct acttttgatt ggaattctgc attcatagat aacattaaat
     1321 tagaaccttt ttccccagag aagatttctt tttgattcga aacatttcct tcattatcaa
     1381 gggaaacttc atattgggtt ttggttcctt gcaggacatc ttcatattgt ttttccaaat
     1441 aactttgtcc tacacggtca ttccgagaat aaccttttgc taggtatttc tctgcttcat
     1501 ctgctggaag tccttgcttt tcagtcgtca cactgccaag aatacttttc agtgaacttg
     1561 ctgcattata ttctcgtgtc cagtctgtgc cagtcgaaac tcctggaagt tcagaagcgc
     1621 gttctgccac aacagctaac tcttgatcag taacaccaga gtttttgata tagacagtgt
     1681 ttaattcata tgcactattc atccgtttaa agatggtagc aatttttaat tgctcctcgt
     1741 ttaaattaat ctcatcagct gtgactttat tgaccatcgc actatattgt tcgctgttcc
     1801 ctaattgctt ttcttttgcc gaaagtcttt ttgtggcttc tttcaaatgt ttgggatcag
     1861 ccagccaaaa atctttttta tctctatctg tcagattttc atctaccgga acatcgatca
     1921 gcgagttcag cttcgttgcg atttcaagca aatctttagc cgtaacttgt gttcctctcg
     1981 tatatgtgat ggcctgattc gctttattcg taactaaagc ttttcctgtg gagtcgtaga
     2041 tcattccgcg aggagtactt tcttggaccg taatcgtaga agtagacttc actcgcgcct
     2101 ccatctcttc gccgttcacg atctgcaaat aacccaatct agcaatcaac gcaacaaata
     2161 acgtaaatat aatgaaaaaa agaaaattta agcgaaaagg aacatgggac cggcgaaacg
     2221 ttttgttctt aggttcttgt gcaccttttc ttattttttc taaccaagaa gtatttaata
     2281 tttttttcat aaaatctttc atcatttcct atccttccgc ctatttacat tcatgcttca
     2341 ttgtacccaa aaacttctta ataaaaaaga aaaaatgggc ggagcaagaa attctttacc
     2401 taatttcaag ataaaatcaa ctttttgttt cacagcaatc acttaaagtt acatatccaa
     2461 ctattttttt cattaaaaaa taaacttatt tttgaaaatt atgttttctt aagtcgtgaa
     2521 acactttgat ggatcattta tttgtggaaa actacgtttt gttattctaa aaaacttata
     2581 taaaaaatag taaggttttt atttcgaatc cataaaatat tcttggattg cttctcgtgt
     2641 ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc
     2701 gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt
     2761 tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg
     2821 cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc
     2881 tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag
     2941 ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca
     3001 tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt
     3061 ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac
     3121 tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga
     3181 ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat
     3241 cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt
     3301 cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc
     3361 aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat
     3421 actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt
     3481 aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa
     3541 tccgcccaac agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct
     3601 ttgaggaata cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga
     3661 aatttaccta ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa
     3721 agatattcaa atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg
     3781 catcttccaa ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg
     3841 cacgtatctt atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca
     3901 tttgcaaacg ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga
     3961 tgattgttgc ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta
     4021 aataagcgac aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat
     4081 gcgtattttc cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt
     4141 ctaattgtat agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta
     4201 gttttggtcc gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat
     4261 ccatagcggc tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt
     4321 tatctgtatt gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt
     4381 cagcatatac tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag
     4441 gacgaacaaa tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca
     4501 aagcacctga tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt
     4561 cacgataaga atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc
     4621 gaagaacaag catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat
     4681 gatcccagtg tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat
     4741 gttggtaacc tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg
     4801 ttgccccttt tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca
     4861 agtcaagttc ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga
     4921 tatattcttt caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt
     4981 gattgtttga gtttccacgc caataagcac ctgccactga taataattta aatacttgta
     5041 tacggccagt ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat
     5101 aagcagtaat cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac
     5161 ttgcaaaaat ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt
     5221 ctttgacgat tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt
     5281 tttgtccatt atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt
     5341 ggatatttgg atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga
     5401 ctaagccaag tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga
     5461 ttggacgagt caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta
     5521 agctttttga aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga
     5581 ctgcaccatc tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt
     5641 gatcagatca tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag
     5701 gacgaaaagt tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta
     5761 acggtccgac cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc
     5821 ctggaaaagt ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct
     5881 ttctcatcaa tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa
     5941 actaaactta ttttttccat tccccattta caccgtatgt tccttttttc atctcattga
     6001 tgatgacatg gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat
     6061 ctttcatcat attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga
     6121 atggcataat gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact
     6181 atgtttgtct tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca
     6241 ctcaaaatag agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt
     6301 agtcccgtac ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac
     6361 tgtttcgcta ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga
     6421 tcatctttca agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc
     6481 attgcaatac cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc
     6541 atcgcaacag gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc
     6601 aagctacggg catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca
     6661 gatccatccc cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt
     6721 gtctttgctg cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga
     6781 tcaattgtta catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag
     6841 gcacgtccaa tctttacttc atgattattg actactgctt ggattccttg accggtcact
     6901 tctttcagtt gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga
     6961 gctaagatat ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt
     7021 tttattccat tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct
     7081 gttttatcaa aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt
     7141 acaacgatcc cgtttttact tgaacggctc attcctgcaa ctaaagcaat gggcgcagct
     7201 agaatcagtg gacaaggtga tgccactacc agtacctcag caaatcgtaa agggtctttt
     7261 gagaaccacc aagaaatacc accgatcaaa taagcaatca acgtgaaagg gactgcataa
     7321 cgatcagcta aacggacaaa tcgggcaggc ttttcttttg attcttcgac tagtttaata
     7381 atcatttgat actggctgtc tgcggcacgt ttttcagcga tcatttggat cgatccgtct
     7441 ccatttaaag accctgacat aatctgatct ccaggtttct tggtttccgg gcgggactca
     7501 cctgtcaaag aagattcatc gaccgaagat tctcctttgg tcactcgacc atctgcgggt
     7561 acaagttcgc caggcttgac tagtaactgg tcatcaatat ttatttcatc gattactata
     7621 tcctgcaatt ttccttcttt gaatcgatgg gcaattcttg gggtgttatc taacaaagac
     7681 tgcagttctt tacccgctcg tttattcgcg taatcctcta gactatctcc gcccgtaagc
     7741 atgatcaata tcatcaaact agcccagtat tctttgacgg ctaatgttgc aaggattgcg
     7801 gtaatcgcta atacatctac gccatatctt cctgaacgta acgtcttgat catttccaaa
     7861 aacatcaaga aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc aagggccggc
     7921 tggtgaaaaa caaattcact taataaagct atcccaccaa taagcaaagt aagtaaaaat
     7981 tttttgaaat gtgtcattgt tttttctcct cttttatgtg acttagagag gttagtaata
     8041 aatttccagc tgcctctttc atcttaagca tagtccttga ctagcacttt ttaaaacaat
     8101 aggccttaat tgaaaacaac tatcactatc agcgtttcca aaattcatca gttctacaaa
     8161 caaaaatatt cagcaaggat tactttttcg attcatcagt taaaaagtac cttttttcga
     8221 aaaactatgc tatactttta attcgttatt tacggcggaa ggagcaaaca tgcgatgaat
     8281 gagaaacaac tagaacaaac gcacgtggat gaaacgatcg aactaatcca gatcgaacaa
     8341 aatatgctgg atacaaaaca acaagaattg actcaggaaa tgaatcattc gacaaaagac
     8401 acagctaatc acaagattcg agctggttcc aatgaagctt tttatgaatc tgctgttgaa
     8461 tatcgccaac atgagcagga attgcttttg aaataccata ctcttgaagc acagcaaaaa
     8521 cgcctgcaaa cgttgtctgt catgaaagat agtccttatt ttgcccgtat cgactttaaa
     8581 gaagaccgtg aaacagaaac attatatcta ggtatcgctt ctttaagaga tctggcagaa
     8641 gaaacaatcg ttattgactg gcgcgcacca attgccaatc tttattatga aggtgagatt
     8701 ggtcccgcat tttatgaaac tgaaacagat agaatagatg tcgatctgca gttgaaacga
     8761 caatttaaaa tccaagaagg acgtatttta tctatggtag atacgtcgga agtaatcaat
     8821 gatgactttt tgctagagat attggatgaa tcatcaagca accatatgaa aaacatcgtc
     8881 tctacgatac aaaaagcaca aaatgcaatc attcgggaca caacaagtaa agtaatgctg
     8941 attgaaggaa tcgccggaag tggaaagaca tccgctttgc ttcaacgtat tgcttttctg
     9001 ttgtatcata atcgcaagtg gctcgaggct gaaaacgttc ttctattttc acccaaccat
     9061 ttgttttctg attacatctc gactgtattg ccttcattag gtgaaagcgg cgtgcctaca
     9121 cagacattca aaaattattt ggtgcagctg ctgccagagt tttctttact agatgaacag
     9181 caacaagaaa gtggtttttt gctaggggaa aaagatccga tacaagtgat gaaaagcgga
     9241 ttgaccttag tcgatcagat tgatcgctat atccaaggca tcacaaacta cggtcccttg
     9301 ttccgtgata tgaaaataaa tggccgtacg atcctatcaa aggaatcgat tcgccaatgg
     9361 tataaagaaa caaatgaaca attaccttta catcatagac tttctctatt acaaacgaag
     9421 ctcctgaaaa aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat
     9481 ctggcggaag aacagctcca agaactatac gcaacagatc caaatcttga atatacagaa
     9541 caaaaggagc gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt
     9601 cgaatccatc gagggatcat gaattatcaa tttgtcaata taacgaaaca atatctccac
     9661 tttctacaaa ctgtgccact ttctcttcta acacgacata aaatcagcga gaccaggtgg
     9721 caggaacatt tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat
     9781 gctgtactat tctttttact gatgcgccga acatatccag tgacggttac acacaaagct
     9841 cgctatatct ttattgatga aatgcaggat ttcgcaccat cacaagtggc attgctgcaa
     9901 gccttgttcc caaaagcaaa tttgactttc tgtggagatc taaatcaaaa tgtctttgga
     9961 aatgaaacga tcgttggatc attggaagac cttttcgtgg accgtgaagt cactcgtttc
    10021 caattaacga caagctatcg ctccacaaaa gaaatcactg attttgctaa tcattttatt
    10081 gtgaaagaac atcaagtaga aacaacagca agaaaaggag accttccttt gctcatccaa
    10141 ggaacaacag atgatatcaa attgaattgg ttagcagata ccattcgaac gactcaggga
    10201 aaagcccggt attggcgtac agcaatcata gctaaaacac aagaagaatg cgaagctctt
    10261 tatgctcaac tgccagacga gttgaaacaa caagtccagc tgattttaaa tgagagtgac
    10321 tttatgaagc gttccattat tttacttcca gcctttttag caaaaggtct ggagtttgac
    10381 cgagttttct tgtgggatgt agatgcagaa catttttctt ctcagcaaga caaattgatc
    10441 ctttacacca tgtgtacacg tgcgatgcat gaattgaccc tttttacctc caataatgaa
    10501 agtcctttgt taaacaatat ggatatttca tcttatgaga aaaaagtact gtaagtaaaa
    10561 agacaactgc tatcattaat gttagcagtt gtctttttat ttacaataat aattgcagaa
    10621 aaagta
//
LOCUS       DAJSBN010000078        10551 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJSBN010000078 DAJSBN010000000
VERSION     DAJSBN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.079"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            23..646
                     /locus_tag="ONT86_002495"
     CDS             23..646
                     /locus_tag="ONT86_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353245.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="ONT86_002496"
     CDS             1096..2145
                     /locus_tag="ONT86_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2353246.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ONT86_002497"
     CDS             2142..3023
                     /locus_tag="ONT86_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2353247.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ONT86_002498"
     CDS             3026..3796
                     /locus_tag="ONT86_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2353248.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ONT86_002499"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ONT86_002499"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR2353249.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ONT86_002500"
     CDS             5107..6270
                     /locus_tag="ONT86_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR2353250.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ONT86_002501"
     CDS             6387..6797
                     /locus_tag="ONT86_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2353251.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ONT86_002502"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ONT86_002502"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR2353252.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ONT86_002503"
     CDS             9370..9654
                     /locus_tag="ONT86_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353253.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="ONT86_002504"
     CDS             9814..10050
                     /locus_tag="ONT86_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR2353254.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="ONT86_002505"
     CDS             10047..10445
                     /locus_tag="ONT86_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCR2353255.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3677 a         1770 c         2232 g         2872 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga a
//
LOCUS       DAJSBN010000079        10086 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJSBN010000079 DAJSBN010000000
VERSION     DAJSBN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.080"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(247..759)
                     /locus_tag="ONT86_002506"
     CDS             complement(247..759)
                     /locus_tag="ONT86_002506"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR2353256.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ONT86_002507"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ONT86_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR2353257.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="ONT86_002508"
     CDS             complement(3212..3655)
                     /locus_tag="ONT86_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCR2353258.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="ONT86_002509"
     CDS             complement(3656..4444)
                     /locus_tag="ONT86_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2353259.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="ONT86_002510"
     CDS             complement(4441..5382)
                     /locus_tag="ONT86_002510"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCR2353260.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="ONT86_002511"
     CDS             complement(5559..6146)
                     /locus_tag="ONT86_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCR2353261.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="ONT86_002512"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="ONT86_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCR2353262.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="ONT86_002513"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="ONT86_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR2353263.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="ONT86_002514"
     CDS             complement(9063..9998)
                     /locus_tag="ONT86_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2353264.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DAJSBN010000080         9613 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJSBN010000080 DAJSBN010000000
VERSION     DAJSBN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.081"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(226..648)
                     /locus_tag="ONT86_002515"
     CDS             complement(226..648)
                     /locus_tag="ONT86_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2353265.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(638..2812)
                     /locus_tag="ONT86_002516"
     CDS             complement(638..2812)
                     /locus_tag="ONT86_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HCR2353266.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2828..5170)
                     /locus_tag="ONT86_002517"
     CDS             complement(2828..5170)
                     /locus_tag="ONT86_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353267.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5192..7735)
                     /locus_tag="ONT86_002518"
     CDS             complement(5192..7735)
                     /locus_tag="ONT86_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2353268.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7745..8143)
                     /locus_tag="ONT86_002519"
     CDS             complement(7745..8143)
                     /locus_tag="ONT86_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2353269.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8224..8508)
                     /locus_tag="ONT86_002520"
     CDS             complement(8224..8508)
                     /locus_tag="ONT86_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353270.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8532..9530)
                     /locus_tag="ONT86_002521"
     CDS             complement(8532..9530)
                     /locus_tag="ONT86_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2353271.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2753 a         2036 c         1492 g         3332 t
ORIGIN      
        1 gatttcaaac aaaatacgtt tattttttct attgatttta gctcaaaatc gattcaattc
       61 gagtgaaatt tgcttcaatt ttcaattggt ttttgttcaa attttgaaac atttttgctc
      121 aaaaaatgag tgaatttgtt tcaaaagaaa aacaaatacg atcgaaaaaa atttgaactc
      181 ataacaattt tttgtttttt tcaacgtatt ctctttcaat tttcactaaa aattaggaag
      241 aaattgattc aatgtctttt caaaaaagaa tgtttttctc tcaatttttt tgacgtttct
      301 atcatgaatc acgagaaaac ttgggacact tttaacatgg taacgttgta aaaaagtttg
      361 aagttcttct tgtcttggtt cttttcttgt ttcaattcga tatacattcc tagagaattt
      421 tttgtcgtat ctttccaaga aagattcaac ttcttggcat tttttacaat catttcttgc
      481 tagatacaca aaaaatgatt gattcgtttt ttgaagttct agtacttctt ctaaattact
      541 attgattagt tttccatttg attcttcgtt agtttttatc agtttaaaac aaccaaataa
      601 gaggaaacta acaataagta tcctaacaat aactttctta tttcgcatat tgtttctcca
      661 aatttggttt ccgaatatag acgactcgcg gtggttcctg gacgtcttca gctgttcgat
      721 acaccacaaa ttcgcctgtt ctcaatgctc gaatgcgatt cggattgaat ttaaagcggt
      781 ctacgtctcg tttcgtgcct ctttctcctg tccaatcaac ctttcccata tcagaataac
      841 ctgcttcttg tgtcgtcatt tccgttaatt caatgtcttc ttctgtcccc attaaatctg
      901 cccaataatc aatttctgtc gggtcatttg tcttcccaat aaagaacgta ttcacattcc
      961 caatcaactg ttttcgaagg acaggatcaa ttctatcaat gtccgcaagc gtttgagggg
     1021 catacgccgc atgcacccca gcgcctcgtc ccttattgac taagtctata aggttatcat
     1081 ttagataaga agctggttcg tcaaatatta ctaggacttt cgagcgattg gctgtttctc
     1141 ctgcttgttt atacacttct gacgcaaaga aattaatatc tcccacaagc atttgagcca
     1201 atgtccgaat aaactcacta taaatcaatc cgtttaacga gatataaaca atctttcctt
     1261 ctttaacaac ttcttgaata tctaattctc tttttcctcc agaagtatca aagagttcac
     1321 caagctcact atccaataac atattgatat tggtctgtaa attctcagaa gtcgcataca
     1381 aataaaacgg gttcttctta tgttcgtaac gaacaaacaa ccgtttaaac aacgtttgct
     1441 cgatttctgt catgagagaa atgttttctt ttaataatag ataatacgtt tctaagtcaa
     1501 cggttttacg atcatgttca acgattcgct cttcacttaa ctgttccaca tcggcaaaat
     1561 cacgttctgt tttgtccatc gcttcattct ttttcgtttt tttcttcgtg gttttcttct
     1621 tttctacgac cactttttct ttaaataaat ctggattttt caactgaata cgatccgcaa
     1681 acaaatgtaa gacgtttcgt ggcaataaat aatgttgtaa ccaaactaga tttctttcaa
     1741 ttttatggct cttttggaac tcgtctaaaa gctgaatcgt tacctgtaat aacgctttgg
     1801 ctgctgtcga ataatacacg ctttctgttt ctgctagatt ggttaattta tctcgaatgg
     1861 caatatcatt gccatttttt acaggattat aacgcaaatc gccaccgtcc acaaagactt
     1921 caatcgggcg attgtatgcc tctgccactt cttttaacgc ttgtaaagtc tctcctgctc
     1981 ctttcccatc gactaagata acggggatac gaaacttaca cgcatgttct gctagtaatt
     2041 ggatcaccgt cgtttttcct gaacctgtag tcgctggaat aatcacatgt tggttcaatt
     2101 cactaaagtt catcacataa gggagcccat actcatctgt tcctaataga atcactttgg
     2161 ctaatgaagc cagttgttta ttccgcaaag caacgtcttt tatgacaatt gctacctggt
     2221 acgcttgctg atactctgct gtatattttt gggactcctg aaatttattc ttccgacgtt
     2281 ccttgtattc tttactattt agcaaagctg tttgccgttc tttctttgaa acaacttttc
     2341 ttttttcaat aaaagacgca tacgcaatca aacaacttcc tgcacctgtc ccacctaaaa
     2401 ggagggcaaa aatcgtttgt ccattaaact gcaacgcatg agaaaacaag tttaatggaa
     2461 ataataagta acacgacaaa taccctctag gaagaacaaa aataaaacaa aatagattta
     2521 atagcgtaaa acctaacgca aaaataaaaa aaagagagct tcttcgtttt aagaagctct
     2581 catactcaaa gtcataaata tgtgcacgtg tctctttttt atctatcata aagacaatcg
     2641 ctaaaggaat actgcctaca ataaaaaatg gatatagaca aacacctaat aaagtgacga
     2701 taaaataaag ttgttgaaac cgactttttg gcgtttcttt ttcttgtttt cttttgcgat
     2761 cgacagaaaa catttgctca aaatgtgtcg gtcgttttgg tgcattcatc atactatcga
     2821 tttcctccta tcgttttcgt tgtttcattt ctcttcgata aatcggttgt ttttctcgtt
     2881 ttcgtttcat ttttgcttga gttgctcgaa actcactccg tgtcttcggt gcttgttttg
     2941 gtgattttgt ttgtttcatg gttgagtatg ctttagatac ggttgaaggc gctttggttt
     3001 gtgctgttac tcgattgact gctgacatat gttcccgaac cccctctgga cgtcgaacag
     3061 atttcggtgt ggtagaacct gttgctcgtg gtacattccc ttttcttagt ggtttttgta
     3121 ccgcatgttt ttgtcctgca actgtccggg ttttcatcga acttgttttt gttacgggag
     3181 taagtgcctc cttccgacga cgacttgttt gtccctgacg acgttttcga tcgttcaatt
     3241 ctgttctagg cgtatgcttc tggccataag acttgtgttt ttgctttgga ttcgctaatg
     3301 acaccaccat tgcagaaccc tgattgcgat acggactacg agacgcacct tttctacttc
     3361 tttctcttag actaggattg ccttgcaatt ctcgttgtaa taaatcggct gtttttcttg
     3421 cctgtttctc cattgctgtt cgttgcatac gttcttgact ttgctcttct gcttgtttcg
     3481 cttgatcatg caagtctctt gctttttgtg ccatacgttc catacgattt ggtgctttat
     3541 gacgaggttg ttgtgcggat tcacgccgaa aagcacttcg tgtggcttgt ttcgccgttt
     3601 gtggcatacg aacgtctgag ccacccccac gttttcgttg acgttcttgt ctcttccgtg
     3661 cgttttcttt ttctttttgt ttctgtgctt ttctcgcttc tttgcgttct ttcaaccgtt
     3721 gtttggcttc ccttctcaat tttgcttctg ccccaaatgg atttcccgca aaagctaacg
     3781 ctcctcgaac acccattctt ccagtaaata gactgcctaa aaagcctcgg aacaaataaa
     3841 ttcccaacgg cgcaaacaaa taaacaatga tcaagagaat tttttcccat ggatcgtctt
     3901 gtgtactact taattggaac ccgtaactaa gaaaagaaga aaaaaggata catgcaaaac
     3961 ctgtcattcc ttttaaaaac gtcaaaatac caaaaacttt ggcatagtta ccaaacacat
     4021 tcattgtgac aagaaacaat cctaaaaaaa gtaagaacgg caacatcggt actaacaata
     4081 gttgcatagt tccaataatc aatcgaaaca tacttaagac aaaataaaca acggtttgaa
     4141 tcagattcac gacaaaataa aacaaggcaa atgctgtatt tttccaacta ttgtaatatt
     4201 gtatgttctt gtttttcagt gtatctgctt catactctgt gactttgtca tgaattttct
     4261 cccctatttt tgtacctaaa tcattgtcca ataaaagatt gattcgatca tattcttttc
     4321 cagcatattt gactttcttc tcacgaattg tgtctacatt ggttgtgcca taatttaaca
     4381 acagatatgg ttcataaaca ttactataaa agacccgagc cgccatcaat ttgcctgccg
     4441 tttccacttt tttatccgct gttacctctt ctgtttcttt tgtttcataa tttgtttctt
     4501 cttcaggtgt taacactgga ttgaccttca cgaacgccgt ttccactgta gaatctaaag
     4561 aaaaaagaac atcaaaaaga ttcttatttg tcccagaatc ttttaatacg cttaacccag
     4621 taaaaataag gatcgtcata agaaagacac ggagtgcttg tttcattcga gcctctccag
     4681 catatttgat tgccatcata attgccgaaa aactgattcc aatagttcct gcaatactta
     4741 acatattgtc cgcaattttt tgtgttaagt tttgaatggg ttctcgaatg gcggaaataa
     4801 tatctaattc aaagagaaat tcaaccattg aagcattgaa ttctcctaac atttttacgc
     4861 cactccaaac aaaatttttc atcactcctg caacattgta cgatagcttt tttttaaggt
     4921 tcattgactc atagtcattg gtcatcaatt caaacgaatt ctttttgtaa tttttataca
     4981 attgatccac atcagtcgtt acttctttta cttctacttt atcgttggca ttctctcctt
     5041 cagaacgatc ggtttcagtg acacccgcac catcattcat ttgatcggca gaaacagtag
     5101 ccccttgtag acagaaaaaa agacccccta ataaaaggag cgtccaccat ttcagagatt
     5161 tttttcgcaa aaaaaccacc tcttttttgt tttatcgttg tttttcattt tctaacattg
     5221 cttgaatttt ttcatcttga ttttcagtag aagaaacagc caataaccaa ctatcaaaca
     5281 acacatcaat ggcaatcggt tgatttcttc ccaaatgatc ctggaataaa caggttcctg
     5341 tttgtaattg ttccatcatt tctaaattgg ctgaattcac ttccatatca aagaaggaca
     5401 atagtttctc tcgtgcttct ttttgtttcg gacgaaacgc aaatttgtaa gaaattaact
     5461 cttttttatc ttcttgatcg taatctttaa acgcttgcac aacaaggtaa atatccgtat
     5521 tattcgcacg tccttttcgc aagctatcat caatcaaata gcttccttgt gcggtatctt
     5581 catacccttt tgcctcgtca taaataatcg cagcgtcttc ttctttgttt gtagaaaaga
     5641 tatacgtaaa cttcataatg acttccataa ttgccattcc tgcaagttgt tcgctattta
     5701 atcgacccga atcaatctct ttttgtgtcg gaatgcgtat tccttgtgtc cctaacacgt
     5761 tgatttgtga atcaaaacgt atcggcgtac tgtgctcatt cccaatgaga atctttccta
     5821 aaccaacgct atgccctaaa atcaatttag ctagttcttt gtcacttttt cgaatttctt
     5881 cgataacttg acttaacgaa tgcttacctg gcatggtgta aactacatcg atggcgtctt
     5941 ggatcaccgt tttcttgtgg ctcgctgttt ctcgattggt atctacttct cctaacattc
     6001 ctaacacatt tttagcggtt tgaattgcct cttcttttgg taaaaacaat agaggatcaa
     6061 gcatccctcg acagctttca tctgaagata acgaaataaa attaattcgt tcatacatag
     6121 ccacaaactc tggtatgtgt ccgaattttt caagtgcctt taaaaagaac ttttgggttt
     6181 catttttcgg atcaatataa agaatttttt gacctaaaaa catcagccat aaaaaaatat
     6241 attttacaag gaccgatttt cctttacctg gtggaccaat aatcaacgta tttccattgg
     6301 tatgttccgc gccttcaata cttttctttg ttaaattggg ataataccat actagtttac
     6361 tagatagcgc taacgcttct ttcgcagatt taaacttttt tgcaataaca atccgtccta
     6421 aaggaaatcc tttcttattt cctacttctt tctttaagtc caagccataa tctgccacaa
     6481 aacctgtcgt agcgacctgc tcgtaactac gaaaacgttg cgtacttcca attaagcatt
     6541 gattaaacaa cgttagctga tccgctaccg atcgataaaa tttgaaatct gtagactcaa
     6601 tcgcaaattc aatatctttg actcgttctt ccaattcttc ttttgaagaa gcagatacaa
     6661 ccagcgtgac ggtagaataa cacaatcgtt tttctttgcg cttcagctta tcttttacat
     6721 ccattaaccg ttctgtacca aaatttaata catcgtctga atctaaaatg ccgtcaacgg
     6781 tctcatattc tttttcttgt tcataaatcc gtccagccat tttattaatt tttcgttcat
     6841 cagattttga atgctcaaat cgaagtcgaa tatgcgtatc aattggaaaa gataagctat
     6901 cctgaagatt ttgaataaag ctactcccat acatggctaa aggagcgtcc gtttgactta
     6961 aaaacgtcga ataatgcgtt tgattcaact gttcaatcgc aatgtatcct gcttcattcg
     7021 aaatgacgcc ctcgcaaatt tccattggcg taagctgtct ttcaggcaat ttttcttctg
     7081 atcgatggaa aaaataataa taaatttgtc caatctcttg ggcagataag cgctctatcg
     7141 gcttataatt ggacaattcc gtatagagct ccttttctaa tgtttcatac gttggtaaca
     7201 cactacttat aggaactcgt tgtcccgtaa tacgatagat aaacttttta aaaacatgct
     7261 taagcaattg aaaatattcg atcggatcag acactttcat ttctgtagtg aaacgcacaa
     7321 acagatacgt tcgatactcg tttaattgca cttcgtcttt taaaatcttt cctgcctgtt
     7381 caaagtacac tttcccaaga tctgcgcatt ctccctttac caaatctcga atggtttctt
     7441 caatgtgcgt atctaggtta aattgacgtg gaatattgat aaaatgatag tcatattcat
     7501 catgtccaag tatgccttcg ccatcttgaa gatagttctt gaaaaaatca atgtcattta
     7561 aaggaaatac ttgggaagaa agttgatagc ctacccacac atcatcattt ttggtcaaaa
     7621 caaaatttcg gataatggcg tctatcggaa attctaatcg aggaagcttc gtttttttct
     7681 ttttgaactg ccaaatcccg ataataaaga cgaccaaaag aaacaaaaac gccatttact
     7741 cgactcactc cttccatttg tgtagggaat acgtcacttt ttcatggaaa aacggcacat
     7801 acatttcatg ttcaaagcgt tgtttccgtt tctgataata ggacgttcgc ccaattaaat
     7861 actcgaaaaa gtgtttattg tcccatttca ttgaaaacaa tgtccaaacg agaacaccaa
     7921 tcccaaaaat cataatcaac caggactttc gaaacagtgt ctgcaagaaa ctaatttgat
     7981 tcacatacgc aatcaaaaat aggatcaaaa gaatgaagag aaatacccca cctatgacta
     8041 attgttccat gcgaatgcca ttagaaatcc agggaatttt ttgtccttta atcccgtaaa
     8101 ttttgtgagg gtgcttaaac tcctttgtat agttgtattc catgttcact ctacctcctt
     8161 ttacaaaaaa agaagcgacc agaagttcta atcttctgat cgcttttgca gtttgtcttt
     8221 ttcttatgat aattgtatcc agggcacaat catttgcgtt acccccacgt tctcttcact
     8281 atccttaata aagataatgt tctttagcca ttcaccaata tcaacggcat tcgataacaa
     8341 taagacgagc attatcaaaa taaagacgcc aataatggca aacgctgccc gcgcaaagcc
     8401 ttgggtcgca taagcaccaa ccattagaac aatcgcaacg attgccaata gccaaatgac
     8461 ttgtccagat acatcatcaa ataacgattt aagatcaaaa agtttcattt ccattctcct
     8521 ctaagttcat tttattcatt taacgtagta acaaaataac tatcgccact tgtttttact
     8581 tccattgaaa agtttgattg gaactcgttt cctttctcaa aatagaaacg atacttccct
     8641 tgcaaaaagt agtttccttc tttatttttt actgcatgat ccagttgtac attgatcaat
     8701 tcgccgcctt ctaacccttt ttcgttagaa atcaatgcca atttttcatc atttttgaca
     8761 tataactcaa aaaaccgttt cacaaatgct tccagcttga tttcttcgct ttcagtgacc
     8821 gcttgcccag aagaataaaa tcgattttcc tgatagttca cagaattggt attcttactc
     8881 tcagaaagat tcaaaaattg aacgccatca agtactttta aaacattgtt ttgataactg
     8941 actggaaaag aaagttgact gcttttccaa tgttctcctt ctgtaaaagt aacctcataa
     9001 aataggcgat acattgcttg tgtcaaatcg atttcttcta gtttaataaa gcgaacatct
     9061 ttcgctactc gattggtagc cgtcgttaca aagcctagat cagacgcttc caatccttgt
     9121 gctaaataat gattgacgga agcctcccat tcgtttcgtt tttctggcga ataagtaaat
     9181 aacctttcaa taaaatgttg tccctcgtac ttcaattgtt ccgtagaaga caaggtttgg
     9241 ttgacttcag ttaacaatgt ttctggattg acttgttttt tcactgctaa acgcgttaat
     9301 gtatccactc gtccaaagtt taggaggagc acgacaccca ttaacagaaa caaacaaaaa
     9361 aaagcgcctg aaaatacact catggcactt ttttgcgaaa attggatcat tcgatctgac
     9421 ggttctgttt tctttttttg gaacaaaaaa cgtttctttt gtttcttttc cttctgttgt
     9481 ttcggtggct tagtttggaa cgcttgtgga ttaaattttg cattactcat gagaaatgcc
     9541 ataccgttct aacaataatt tcggtatttt gtccacaatc actacttcgt ggacaaccac
     9601 acttgccccc tcc
//
LOCUS       DAJSBN010000081         9361 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJSBN010000081 DAJSBN010000000
VERSION     DAJSBN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.082"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(92..760)
                     /locus_tag="ONT86_002522"
     CDS             complement(92..760)
                     /locus_tag="ONT86_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2353272.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            894..1181
                     /locus_tag="ONT86_002523"
     CDS             894..1181
                     /locus_tag="ONT86_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353273.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1597..3135
                     /locus_tag="ONT86_002524"
     CDS             1597..3135
                     /locus_tag="ONT86_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCR2353274.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3132..4097
                     /locus_tag="ONT86_002525"
     CDS             3132..4097
                     /locus_tag="ONT86_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2353275.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4084..5406
                     /locus_tag="ONT86_002526"
     CDS             4084..5406
                     /locus_tag="ONT86_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2353276.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5469..5573
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5647..6390
                     /locus_tag="ONT86_002527"
     CDS             5647..6390
                     /locus_tag="ONT86_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2353277.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6405..7397
                     /locus_tag="ONT86_002528"
     CDS             6405..7397
                     /locus_tag="ONT86_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2353278.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7409..8146
                     /locus_tag="ONT86_002529"
     CDS             7409..8146
                     /locus_tag="ONT86_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2353279.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8136..8858
                     /locus_tag="ONT86_002530"
     CDS             8136..8858
                     /locus_tag="ONT86_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR2353280.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8942..>9361)
                     /locus_tag="ONT86_002531"
     CDS             complement(8942..>9361)
                     /locus_tag="ONT86_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCR2353281.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2714 t
ORIGIN      
        1 tttttttata aagaagattt tactgaataa tatcatttta gtttacataa tgatatttct
       61 gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa
      121 gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt
      181 ccagatactt cactttattc aatgtataag atagaataga acagctaagt aatgtataca
      241 gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga
      301 aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg
      361 ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac
      421 ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc
      481 ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt
      541 ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag
      601 aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg
      661 ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa
      721 tgattccgca caattctgta aataaattta attttttcat ttctagcctc ttttcttttg
      781 aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga
      841 aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag
      901 cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt
      961 ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc
     1021 aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt
     1081 ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc
     1141 tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag
     1201 acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca
     1261 gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat
     1321 tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca
     1381 agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt
     1441 tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct
     1501 tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa
     1561 tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta
     1621 agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt
     1681 tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa
     1741 aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc
     1801 ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt
     1861 gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac
     1921 ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa
     1981 gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta
     2041 atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt
     2101 ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc
     2161 ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat
     2221 tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct
     2281 gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc
     2341 ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa
     2401 ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc
     2461 tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa
     2521 agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa
     2581 ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag
     2641 agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat
     2701 cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc
     2761 caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac
     2821 caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc
     2881 aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc
     2941 gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat
     3001 aaattaagcg gaaaaataac ggaggatcaa atgattgata tgaattacca agtcaatgtc
     3061 gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag
     3121 gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa
     3181 agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg
     3241 gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat
     3301 cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg cagaaaatgc
     3361 ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc
     3421 aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg
     3481 tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta
     3541 gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc
     3601 cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat
     3661 tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc
     3721 acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa
     3781 ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc
     3841 atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg
     3901 gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta
     3961 tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa
     4021 gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag
     4081 gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct
     4141 tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg
     4201 acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt
     4261 aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa
     4321 gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa
     4381 ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct
     4441 attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga
     4501 cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca
     4561 ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta
     4621 gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca
     4681 ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc
     4741 attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt
     4801 ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag
     4861 ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt
     4921 gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt
     4981 tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag
     5041 cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac
     5101 gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa
     5161 gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct
     5221 aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag
     5281 caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa
     5341 gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg
     5401 aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa
     5461 aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag
     5521 acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg
     5581 ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt
     5641 aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg
     5701 cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta
     5761 ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat
     5821 gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg
     5881 gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg
     5941 ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa
     6001 gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg
     6061 aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag
     6121 atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg
     6181 gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc
     6241 ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg
     6301 atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa
     6361 gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc
     6421 ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca
     6481 aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta
     6541 atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg
     6601 tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag
     6661 cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat ggacagaata
     6721 taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc gccaccctat
     6781 cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa
     6841 gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa
     6901 aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata
     6961 tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa
     7021 actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta
     7081 cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga
     7141 aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat
     7201 atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt
     7261 atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag
     7321 cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta
     7381 atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt
     7441 cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac
     7501 atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt
     7561 ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac
     7621 agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa
     7681 cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta
     7741 tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg
     7801 gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct
     7861 tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt
     7921 gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc
     7981 aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga
     8041 gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa
     8101 gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt
     8161 tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca
     8221 attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag
     8281 ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc
     8341 cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg
     8401 taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg
     8461 agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag
     8521 cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa caagactata
     8581 ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc
     8641 aactagctaa agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag
     8701 cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac
     8761 agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt
     8821 atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag
     8881 tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat
     8941 cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg
     9001 aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt
     9061 aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc
     9121 ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag
     9181 tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt
     9241 gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt
     9301 gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt
     9361 a
//
LOCUS       DAJSBN010000082         9213 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJSBN010000082 DAJSBN010000000
VERSION     DAJSBN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.083"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            4..2376
                     /locus_tag="ONT86_002532"
     CDS             4..2376
                     /locus_tag="ONT86_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353282.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ONT86_002533"
     CDS             2437..3897
                     /locus_tag="ONT86_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR2353283.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /locus_tag="ONT86_002534"
     CDS             3908..6112
                     /locus_tag="ONT86_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCR2353284.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            6133..6282
                     /locus_tag="ONT86_002535"
     CDS             6133..6282
                     /locus_tag="ONT86_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353285.1"
                     /translation="MRNTTHKEQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     LELDR"
     gene            6358..6951
                     /locus_tag="ONT86_002536"
     CDS             6358..6951
                     /locus_tag="ONT86_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR2353286.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            6967..7866
                     /locus_tag="ONT86_002537"
     CDS             6967..7866
                     /locus_tag="ONT86_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR2353287.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            7869..8354
                     /gene="ssb"
                     /locus_tag="ONT86_002538"
     CDS             7869..8354
                     /gene="ssb"
                     /locus_tag="ONT86_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2353288.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            8720..8980
                     /locus_tag="ONT86_002539"
     CDS             8720..8980
                     /locus_tag="ONT86_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353289.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         3464 a         1547 c         1926 g         2276 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgct tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaga agatgtttga acaccagaga
      121 aagaccaacg gtcagccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gaacgtttag aaaacaaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcggc
      301 ctggatatga caatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc agaaaaagga aaagaaaatc ttcttcctga agtacaagca
      481 attattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 gactatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctttgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgaa
      961 aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtgaaag aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc tgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacggggg gcatatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac ttcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccacagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 tcattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaatg agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac taaatcgtga tcagttaaac
     3181 gatacgtggt tagtttcgat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtc gcaaaagaag aaaagatcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaatt ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagcttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggattg gaatgatcaa ttgaagcatg aaatctacca acaagagatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggtagac agcaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaaaccgtca caagcgaagg cttatgccga
     3961 tagcttttct aaagctaccc gaaaagatgg gtattttgag atccaagatc gattgttttc
     4021 aggagaaact gtgatcacgt atggttttgg tcatttagtc gagttagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agagcatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatagcttg
     4261 gacgatcatt caacaagcag gcgccgatca tggaaagata tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagatg cagaagagac
     4381 ctacccaaaa tttgaagaag cacaagcacg ccagatcgcc gattggttga tcggcatgaa
     4441 cggtagcccg ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gtttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttacaggaaa agattagaaa
     4561 cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggtacg atccaccaag ttgaaaccga
     4741 gaaaaaaagt gcggcaagtc cacgtctttt ttctctatcc agccttcaat ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagatac tttggaagcc atgcaagggt tgtatgaagg
     4861 gaaatatcta agctacccac gaacagatac cccttatatt acagaagggg agtatgcgta
     4921 tctgttggat cacttagacg aatacaaaca ctttttaaag gccgaagcca ttcctactcc
     4981 tattcataca ccgaatagtc gatatgtgaa taataagaaa gtccaggaac attacgcgat
     5041 cattcctaca aagacggtca tgacggcagc ggcttttgaa caattaagtc cactgcagca
     5101 ggctatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa agtataccta
     5161 tgaagaaaca acgatcttga cgcaagttca gcagcttcag ctaaagacag tcggaaaagt
     5221 accagtagat ctaggttgga aaaagctgtt tggcaaagag agcgacagaa aagaaaagga
     5281 agaagaaccg ctacttccta aagtgacaaa aggagaagcg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaacgaagc caccacaacc ttataccgaa ggtacgttga tcacagcgat
     5401 gaaaacagct ggaaaaaccg tggatagcga agaagcacaa tccatattga aagaagtaga
     5461 aggaatcggt acagaagcaa cacgagcaaa tattattgaa actttgaaac aaaaggaata
     5521 tatcaaggta gaaaaaaata agcttgtggt gacaaataaa gggattttac tttgccaagc
     5581 cgtggaaaaa gagccgctgt taacaagtgc cgaaatgacc gccaaatggg aaagctatct
     5641 tacaaagatt ggtgagcaaa aagggacaca agctactttt ctagcaaata tccaaaaatt
     5701 tgtttcccac ttgttggaag tcgtcccagg acaaatccaa gccactgatt ttggagcaac
     5761 gttacaagag gtgaaagtag ccagtgaaaa gcaagaagcg gcgcgtcatt taggatcttg
     5821 tccaaagtgc caggaaaaag aagtcatact ttatcagaag gtggcgactt gcacgtctga
     5881 agcctgtgat tttaaattgt gggtaacgat tgccaaaaag aaactcacag cgacacaact
     5941 gaaagagatc attcagaacg gccgaacaag tcaaccagtc agaggactaa aaggtcagaa
     6001 aggttcgttt gaagcaacga tcgttttaaa agaagacttt acaacaggat ttgagttttc
     6061 tgaaaagaag aagaccacct acaaaagaaa agtgagacgt aaaacgagtt agaattgacg
     6121 gagggaaaaa cgatgagaaa tacaacgcat aaagaacaag aaatcgaaaa tcaaaaagcc
     6181 aaaatacgtt cagagcagga gaaaatgcgt gccagcaaac gaagactaca gcaattgaag
     6241 aatcaacaac ggattcaaaa agaacttgaa ttagatcgat aaatcctcct gttgttaata
     6301 ttaaactaac atgttatagt tatgttagtt taatattaac attcgagagg gaaagcgatg
     6361 aaagataaag tcaagtgcat ttttgagaaa tacaatggta atctatcctt gaaggatgct
     6421 agaaatgaag ggttatcacc tatgacgtta gatcgtttag ccgaaaagga caaaatagaa
     6481 aaaatagcat caggttttta taccttaaaa gatagttttg tggatgaatt atatctcgct
     6541 caaagtatct atactagagg aatcttctct cacgagacag cgcttgatct ttttcaagta
     6601 ggaacatata tccctaaaaa aattaatatg atgtttccta aaggatacaa tgtttcgaaa
     6661 gagaatttag atcagtatgc cattcaaccg cattacacga ataaagaaaa ctttgaactg
     6721 ggaatcacag aggcagaaag cttttacggc aataaaattt tcgtctatga tttagagcga
     6781 acgttatgtg atatgtggaa tcccagatat aaagcaagcg tagaagtaaa acaagaagca
     6841 ttaaaagaat atatgacaag taccagcaga aatacaaata agctaagaag atacatgaat
     6901 attttaaaat caccaaaaga aatgactttg tatatgttgc cactttattg agaggagtaa
     6961 aggtgaatga caccgacaca atttaaagcg aaaacaagaa atgttgcagc tgagaaacat
     7021 ttaaacccac aaattgttca aagatattat atgatggaaa aattattaga atgtattgtt
     7081 gaatcgcctt atcatgatga ttttgtatta aaagggggat ttctgatcgg atcaaaatac
     7141 gggttagaaa accgttcaac cgttgatatt gatacgactt atcgaaattc gaaactaaca
     7201 aaagaaaaat tagaaactgt tttttctgaa ttaacagctg ctcctacaaa agatggcatt
     7261 cggtttgaat taaagggctt aacagagacg agggaagccg attattaccc aggttttcaa
     7321 gcaaagttta ttgcttttct tgaaaatgca cgtgtacctt ttaaattaga tattacttca
     7381 ggtgattcta ttattccagc agcattgata tatgaacata aattgatgtt tgaagataaa
     7441 acaattcata ttccagctta tcctacggaa caaattttag ctgaaaaatt acatgccacg
     7501 tttagtttcg gaatagataa ttcacgtatg aaagatttct atgatctgta tatgatccct
     7561 aaactagaac gtatcgatga gataaatttg tataagagtg ttcaaggaac atttaaggaa
     7621 agaaataatc aatcagtgtt taagaatttg tataatcagg aaatgcctca attgatgaag
     7681 aatgagggat tgagggagaa atggaaaaga tatcagcaag aaaactattt tgccaaagat
     7741 atttctttcg aatctacctt aacttctgtt gattcgctga tgcaatcaat tgtcgagcaa
     7801 gaggaagtag aacgaaaaca aaattttaag agatcacaac aaatgaatca agaaatggag
     7861 agataaagat gatcaataat gtaactttag taggtcgatt aaccaaagat cccgatttac
     7921 gttacaccgc aagtggcact gcagtgacca cttttacctt agccgttaac cgtaatttta
     7981 ctaatcaaag tggcaatcga gaagctgact ttatcaattg tgtgatttgg cgcaaaccag
     8041 ctgaaacgat agcgacatta gctaaaaaag gcagcttgat cggcgtcgtc ggacggattc
     8101 aaacgcggac ttatgacaac cagcaaggac agcgtgtcta tgtgactgaa gttgtggccg
     8161 ataatttcca actattagaa agtaaagcag cgacagaaag ccgtatgcac gctgatcaat
     8221 cgtccagcag tccaagccct accacctttg aacaacggga tacagcgacg cctaacaaca
     8281 atggattgaa cgcttcacag aatccatttg gtggacaatc aatcgatatt agcgatgatg
     8341 atctaccgtt ctaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt
     8401 tagacaacaa aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggc
     8461 tttcttttac gtttctatta taagaattaa cgggtaggct tactgtcttt cttggcaaat
     8521 ttacaatcaa gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca
     8581 aaatcaaaaa gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag
     8641 cagacagcaa gactattata gcataattga aatatccaaa tgattggatt tttgctataa
     8701 ttttagcgag gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat
     8761 aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg
     8821 tgtagttgca gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa
     8881 tattgccgtc acaggtcact tcaataaacg aaatcagcta gttattgcta gcatgtatgt
     8941 tagaaatccc gatcatttta cgcgatcgat gggcatataa ataaaagaag agttgtagtc
     9001 ctaaatgagg attgcagctc tttttgtttt acatagaaac aggaaaagac ctttttagga
     9061 agacacaagt gccttctcaa ttgaaaaacc tttctctcac tccaaccgcc accgccgttc
     9121 gagaagaaag gaagtgaacg ctgcacgttc actaagatat ggtgtcttgc aaagcaagac
     9181 aaaatagaca aaacttcctc tcaccaaaag gtt
//
LOCUS       DAJSBN010000083         9048 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJSBN010000083 DAJSBN010000000
VERSION     DAJSBN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.084"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ONT86_002540"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ONT86_002540"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR2353290.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="ONT86_002541"
     CDS             2714..2938
                     /locus_tag="ONT86_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353291.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            2951..3451
                     /locus_tag="ONT86_002542"
     CDS             2951..3451
                     /locus_tag="ONT86_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR2353292.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            3548..5395
                     /locus_tag="ONT86_002543"
     CDS             3548..5395
                     /locus_tag="ONT86_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HCR2353293.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            5398..6294
                     /locus_tag="ONT86_002544"
     CDS             5398..6294
                     /locus_tag="ONT86_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2353294.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            6344..6733
                     /locus_tag="ONT86_002545"
     CDS             6344..6733
                     /locus_tag="ONT86_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2353295.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            6720..>9048
                     /locus_tag="ONT86_002546"
     CDS             6720..>9048
                     /locus_tag="ONT86_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2353296.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         3196 a         1362 c         1909 g         2581 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattggtttg tttttaggat ttaaacgcga
     2821 gttttccaaa ctgattggct tcttggtgat tgctttgatt gcggtaggtc tggtctttaa
     2881 tgcttctggt gtgaaggatg tcttactcaa tttgtttaac cgaattgtcg gtgcctgagg
     2941 gggaatcaca atggaacaga tacgtgttta tattgcgaac cttggaaaat acaatgaagg
     3001 tgagttggtg ggggcatggt ttaccccacc cgtcgatttt gatgaagtga aagaacggat
     3061 tggattaaat gacgaatatg aagagtacgc aattcatgat tatgaactgc cttttgagat
     3121 tgatgagtat acaccaattg aggaaattaa tcggttgtgt ggtttagcgg aagagcttaa
     3181 aggaacgcca attggtgagg tcgcttcaga gattcaacat gccttcttca attctttcga
     3241 ggaaatggta gagcatgtgg atgatatcat ctattatccc gattgtaatg acatgagtga
     3301 tgtagcgtat tacttgattg tagaagctgg tgatttaggc gaagtaccag aacacttgaa
     3361 gacgtatatt gattttgatg cgtgtggtcg agacttggag attggcggga attatttggt
     3421 tacgagtcga ggggtttttg aactaatgta aatcatttgt tagaattgac cagaaatttc
     3481 tatcgtactt ttacttttct atttgatata ctgaaataga aaatatttgt tgataggggt
     3541 gttactaatg gttggcaata agtggtacaa atgtgatctt catattcatt caactgcgag
     3601 tcagtgcttt caggataaaa aggttactgc taaggaatgg gtagaagaat gtttaaacaa
     3661 aggactcgat tgtgttgcat taactgatca caatacaggg gaaaatattg atgaatataa
     3721 gaaagtggct atcgaaaatg gcttagattt ttttcctgga gtggaattaa cgtgtggaga
     3781 aaatggaact catttactag tcctatttga taaaagtgca tgtacaaatg atatctctga
     3841 tttcttaacc aagatgggta taaaaagaga ggattgggcc agcgctgcag cttgctcaat
     3901 gaaatcagtt ttagaagtta ttgattttgc tgagcacgag ggcttgctcg ttattcctgc
     3961 acatatagat gagtttaatg gaatttcttc agcaaaattt ggaaatctag gaaagatatt
     4021 tgaatcagaa aatatatatg cagtacagac ggttcaaaaa gaattttttg aaaatcgctc
     4081 acaaatgatt ccctcatcta agcgtgacac tatctatgat agtgtgaatg ccagatatga
     4141 tggtagagtt ggaaaagata ctttggaatc ttggtataaa tcagttgttg aggcagaaaa
     4201 aaataatatg acatttctta gcttttctga taacccacac tcgaaaggaa attcaaaaca
     4261 tggactttgg ggaataggca caagatattc ctatataaaa atgaaggacg aacctgactt
     4321 gggatcactt cgcgatgctt taatgctagg cgaaactcga gttcattctg atttttctaa
     4381 tttctccatt aatgaaaatg aagttttatt ggaaaaatta agcttttctg ggaccactct
     4441 tagcacaaaa gaagttgtag tcgaattcag cgataattta acttcaatta ttggaggaag
     4501 aggaacaggc aaatcttgta ttacaagatt cttggtctat gtccttggaa aggaagcaga
     4561 acttgatcaa ttttctgaaa tacaaagtga ttatcagaac tttgcacaaa ttgaacataa
     4621 tggctctgga atatttttga aggatacaat tgtgaaactg aatattttct ataaggggac
     4681 aaaatatcaa ataataagaa cacaggatag acattctatt tatgaattta cacaagagaa
     4741 tggtctggtt gaaaaagaaa cagaacggtt gagaatgatt tccgataagg tgagtatata
     4801 ctctcaaaaa caaatttatg aaatttcaaa aaatcaaact agtatactag aattactaga
     4861 tggatataac tctgatttga tttcagagta taaaaatgag attgaaacat gtgtaaacga
     4921 aataagaaaa ttaaattggg atattgtttc tgtgaaaaaa gaaatacaag ataaggcgaa
     4981 ggtagagctt gaaattgaag atttaaaaaa acaagtagaa aagctatctc ataagagtta
     5041 taaagatgtt tatgatgagt attcaaaaga gacagatatt tatagggaac tgaaacgtga
     5101 tgtggaggca cttaaggaaa ttcccaaaaa tctaactgat agtgttgaca aaattgactt
     5161 tggcaatggt ataaaagtta cagacgaaat tgatgaacac cgcggagaat tgattaagaa
     5221 tttagtgacc aatcgggcta agattcaaac aataattaat gaaatgtcag atgaaattga
     5281 tagttataga gcagagctga ataaatcaga ttggaaagtt aactataatg aggtttcaaa
     5341 gaggtatcaa agaggtatca aaaacttagt gaagagttat ctcaagatga attaaaaatg
     5401 ttacaaaacc ttgattcttt atatcaatct ttatcaaaaa ctgaagagcg attggaagaa
     5461 attattcaac aagaaataaa gcttaaagat taccaagttg aggttgactc agcaatgtca
     5521 agaatgaata aatcatatca aaatctctat tctgagagaa gtagttcctc gcgcgaaatt
     5581 ttttctgata ttacaggaat agaggctact attcaaccaa atagggattt caataattat
     5641 gttaaagagc tcagagcttg tattaataag gaggacactt ttgacagtga gtttggatct
     5701 cttattgata agctgacaaa aaattccatt actaatcaag atatatataa tgacatactt
     5761 gatataagag ttagtgggga aagtagtatt ttttcagata aaaaattaat caattctata
     5821 agaaaattga atgatgcaca agttcttgaa ttacaaaccc ttaagccaaa tgatttagta
     5881 gttatccgac ttgaactaaa tggaaaaaaa gttagtttat cgaatgctag tgctggtcaa
     5941 aagacatctg caattctttc tatgattttg gcgtatggaa attcaccact catattggat
     6001 cagcctgaag atgatttgga tagtcaatta attaataact taatcgtaag aagtatcatt
     6061 acaaaaaagg aaaacagaca aattttaatt gtaactcata atgcaaatat cccagttaat
     6121 ggtgattcag aatggttgat tagcatggga gattcaaaag aaatatcggc tgatcagtgc
     6181 gggtcggtag atgaacccaa tatcaagcca agaatatgta ctgtgatgga aggtggcgag
     6241 gatgctttta caaatagagc gaaacgctat ggttttaaac aaattgtaga ataacactag
     6301 agggtgagac atattattat gtccgccctt tttaggagga aaaatgaaaa aaataaaaag
     6361 ctataccagt atctgggcag ttgaaaaagt catttacgca atcaatgatt ttcagttgcc
     6421 ttttcctgtg acttttaacc aaatggcttg gttcgtttta tctttactgt ttgtcatcgt
     6481 atttgcccat gtgcctccac tatctatgat agaggaggtc tttttaaaat acttaggcat
     6541 accggtggct gtcacttggt ttatgtcgca aaagacgttt gatggcaaga aaccactggg
     6601 atttttacga tcctttatca gctatcactt gcgatttaag gtgacttttg caggaaagaa
     6661 agtcaaagaa cagaagaaac gatgggatga acccagtact cttgtaagga gtgtgaacta
     6721 tgtatccgat taagtatatt gaaaataatc tcgtctttaa ccaagaaggc gagtgttttg
     6781 cctactatga attagtgcct tacaattact cctttttgtc acccgaacag aaatttcaag
     6841 tgcatgataa ctttcgccaa ctaattgcgc aaaatcgtga aggcaagatc catgcgttac
     6901 aaattgccac agaaagtagt attcgcgcca cacaagaacg atcaaaaaaa gaaattaccg
     6961 gtagactcaa agaagtagcg aaacaacgaa ttgatataca gacagatgca ttagtatcca
     7021 tgattggcga tagtcagatt gattatcgct tttttattgg ctttaaacta attgcgacag
     7081 atgaagaagt caatctaaag agtctcaaga aatctttctt ttctgggctt caagaatttg
     7141 tctatggagt gaatcatcat ttgatgggcg attttgtttc cttatccaat gaagaaattc
     7201 gctgctattc taagcttgaa aaattaatgg aaagcaaatt ggctcgaaga tttaaagtca
     7261 ggcgagtcac accgagtgac ctgacgtatt tgattgaaca tatttatggc gaaaaaggga
     7321 taccgtttga agaatacgaa tttcagctac caaagaaaaa gctaaaatca gaaacactgg
     7381 tgaaacgtta tgacttatta cgtccaaatc gttgtttaat tgaagaaaaa ccaaggtgct
     7441 tacggatgga acatgagaat catgagtcgt acgtagcgta tttaacaatt aatacaattg
     7501 tgggggagat ggaatttcct tcctcagaac ttttctatta ccagcaacaa caatttacat
     7561 tcccaatcga cacaagtatg aatgtagaga ttgtgacgaa taaaaaggct ttagctaccg
     7621 ttcgtaataa gaaaaaagaa ctgaaagatt tagacaatca tgcttaccaa tctgacaatg
     7681 aaacgaattc caatgtctta gatgctttgg attccgtgga tgaattggaa accaccttgg
     7741 atcagtcaaa agaatctatg tacaaactga gttatgtggt tcgagtgagt gccgaatcag
     7801 tggatgaact aaagcgacgc tgtgatgaag tgctcgattt ttatgatgat accaatgtaa
     7861 aactagtccg gccctttggc gatatgatgg ggctacatga agaattctta ccctcaagta
     7921 aacgatatat gaacgactat attcagtatg ttacgtctga ttttttggcg gggcttggtt
     7981 ttggggctac tcagatgcta ggagaattag aagggattta ctttggctac aatgtggata
     8041 caggtcgtaa tgtttacctg aaaccggcat tggcttcaca aggagtcaag ggttcagtaa
     8101 ccaatgcgct ggccgccgct ttcctgggtt ctcttggtgg aggtaaatcc tttagtaaca
     8161 atctcttagt ctactatgct gttttgttcg gtggtcaagc tgtgattgtc gatccaaaag
     8221 gggagcgagg agggtggaaa gaaaccttgc cagaaattgc ggatgaaatc agtatcctca
     8281 acttgacgag taagtcagaa aatcaaggtc tgcttgatcc ttatgtgatt atgaagaaaa
     8341 agaaagactc ggaaagttta gcaattgata ttctgacttt tttaacagga atttcaagtc
     8401 gagacgggga gaagtttcct gtgttacgta aagccattcg aacggtgact caaagtgatc
     8461 aacgggggtt attacaagtt attaacgaat tgcgaaaaga cgggtcacca atcgcggaaa
     8521 atatcgccga tcatatcgag tcctttaccg attatgattt tgctcatttg cttttctcag
     8581 acggctcgat tacccaatcg attagtttag ataaacagct aaatatcatt caagtggcgg
     8641 atttggtttt accggatgca gaaacgactt ttgaagaata taccaccatg gaattgctta
     8701 gtgtttcgat gctaatcgtc atttccactt ttgccttgga ctttatccat agtaaccgag
     8761 aaatcttcaa aattgtcgat ttggatgagg cttggagttt tcttcaagtg gctcaaggaa
     8821 aaacgttatc gaataagctt gttcgtgctg gtcgggccat gaatgctggg gtctatttcg
     8881 tcactcaaaa tagcgacgat ttattagatg aacgtttaaa aaataatatc gggttgaagt
     8941 ttgcttttcg cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggtttgg
     9001 atcaggagga tcaaagcaat caaaaacgcc tgcgagattt ggaaaacg
//
LOCUS       DAJSBN010000084         8851 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJSBN010000084 DAJSBN010000000
VERSION     DAJSBN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.085"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            63..1277
                     /locus_tag="ONT86_002547"
                     /pseudo
     CDS             63..1277
                     /locus_tag="ONT86_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1456..1785)
                     /locus_tag="ONT86_002548"
     CDS             complement(1456..1785)
                     /locus_tag="ONT86_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR2353297.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1775..2047)
                     /locus_tag="ONT86_002549"
     CDS             complement(1775..2047)
                     /locus_tag="ONT86_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR2353298.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2498..3301)
                     /locus_tag="ONT86_002550"
     CDS             complement(2498..3301)
                     /locus_tag="ONT86_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR2353299.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3279..4403)
                     /locus_tag="ONT86_002551"
     CDS             complement(3279..4403)
                     /locus_tag="ONT86_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2353300.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4419..5234)
                     /locus_tag="ONT86_002552"
     CDS             complement(4419..5234)
                     /locus_tag="ONT86_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2353301.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5224..6096)
                     /locus_tag="ONT86_002553"
     CDS             complement(5224..6096)
                     /locus_tag="ONT86_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2353302.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6181..7470)
                     /locus_tag="ONT86_002554"
     CDS             complement(6181..7470)
                     /locus_tag="ONT86_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2353303.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7698..8342)
                     /locus_tag="ONT86_002555"
                     /pseudo
     CDS             complement(7698..8342)
                     /locus_tag="ONT86_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2742 a         1883 c         1410 g         2816 t
ORIGIN      
        1 caaaatcaat tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag
       61 ccaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa
      121 gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatgggaaga
      181 aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttacg cgcctaaaaa
      241 agcaacgcta tacctgtaaa aactgtcgta cccattggac ggctcaaagt tattttgtcc
      301 gactcagcca ttcaatcgca gatcatgttc gatataaaat tgcttcttta ctgactgaaa
      361 aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc tttgactacc gttactcgta
      421 cattgaaaga gtttaaaagc tatttaccaa agccttctaa gaagattctc cccagagtat
      481 tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg
      541 cagatggtga aacaggaaac ttaattgctg tcttgcctac gcgtaaatta cctcgattaa
      601 caagctattt cttaggctgc accaatccag aagaagtaga attcttagtc acagacatga
      661 acgccgctta cttccagctt accaaacgtg ttctgccaaa tgcgaaagta gtgattgatc
      721 ggtttcatat tgtcaaacac atgaatcagg cactcaatga gttgcgtatc cgtgaaatga
      781 atgagcttcg taaagcagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct
      841 ttctgctaaa aaatcgtgca agcatcaacc attatgagta caaaacatgg aaaagtttcc
      901 gagcaccaaa atacccattt ctcactgaag caatgatgat tgatcgattg cttgagtttt
      961 ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt agtcgaagcc tttcgagaca
     1021 aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacggct
     1081 ttcgggagaa gcttcaaaac cttctgacct atgaagaagg tatcaccagc gcaatgatct
     1141 atccttattc caatggaaaa attgaagcga agaataccca cataaagaca atgaaacgag
     1201 tatcctacgg atttaaatca ttcgagaaca tgagaattag aatctttttg atcaatcaat
     1261 taatcaatgt aagataacaa aaaatctgaa ccagaatgtg gttcattctg acccggattt
     1321 gattttgcac aactcatcag tccttattga caaagagcca aaaatctgag ccagaatgag
     1381 gttcattctg acccagattt gattttgtac aacccatcag tccttattga caaagagccc
     1441 ttctttttag tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg
     1501 acttaattcc ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag
     1561 agttcctacg tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc
     1621 aacgtatttc agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa
     1681 cgaaattgtg tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc
     1741 agctgtgaaa gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt
     1801 cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa
     1861 tattatggat tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct
     1921 caacaatttg cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa
     1981 taatgctttc tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt
     2041 tctccacctc tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac
     2101 aactactttt gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag
     2161 tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg
     2221 aacactcaac gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg
     2281 tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg
     2341 aagaccgata tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg
     2401 tttattgata gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta
     2461 aaaagtgaat gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt
     2521 ttttcatgcg ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt
     2581 ttccgtcaat taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg
     2641 ggtattcatc tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt
     2701 cagcactgct accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa
     2761 tcaattcagc aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg
     2821 gatgatgaac caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt
     2881 gaatcatctg tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg
     2941 aaataattcg taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt
     3001 caacactcat tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt
     3061 aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc
     3121 ccccttcatg tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt
     3181 cttgtaacaa tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag
     3241 taaacaagtt agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca
     3301 tggctattcc ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga
     3361 taatcaaaaa aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc
     3421 caagtaggtt tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca
     3481 taactttgac ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt
     3541 gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt
     3601 tgtttttgcc aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga
     3661 tcataagtaa ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta
     3721 tttttaggtt tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc
     3781 aataaaacaa tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca
     3841 aaagtttgtt tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa
     3901 cgcacatcca aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc
     3961 gcacgagcca atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct
     4021 tggtatggcg ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct
     4081 gtcttaattt tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga
     4141 tataaagcat aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt
     4201 gctagttgtg tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt
     4261 ctcagcaaag tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg
     4321 gccacagcta aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt
     4381 cttaattcaa gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca
     4441 aaagtcccga taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta
     4501 attaaactaa tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc
     4561 tgcatcgcca acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca
     4621 tactcattcc aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta
     4681 agaggaaaaa caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca
     4741 tctagaaggc tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca
     4801 tcagcaaatt gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc
     4861 aacaaataat taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata
     4921 aagtaccaga ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa
     4981 gctacgccta acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt
     5041 ttgataattg gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt
     5101 atcaaactag taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata
     5161 aacaataatg gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag
     5221 cgtttaattt tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac
     5281 agcaaatatt aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc
     5341 aaaagccttt tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg
     5401 agtgatgatc ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa
     5461 taaatatagt cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat
     5521 gggtacacga tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc
     5581 aaaaatgaca atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc
     5641 aattacccaa ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa
     5701 gacagcaatt ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac
     5761 caaagaaatc acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat
     5821 aactattcct aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag
     5881 aacggtatag gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt
     5941 tacatagttg gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa
     6001 actcaaatag atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc
     6061 tggcgccaga tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat
     6121 gtcaaaagca gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc
     6181 ttatttgagt aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt
     6241 acctgataaa attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc
     6301 tccaggaaat gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat
     6361 ctgattttca gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg
     6421 tggtaaataa ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt
     6481 taagaaatcc caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc
     6541 gccaccagca ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc
     6601 agtaaaagta ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc
     6661 gacgttacca tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg
     6721 gtcttttacc atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt
     6781 agctttgcca tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca
     6841 attatcagca ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt
     6901 agaaaaagtt tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt
     6961 taatatatct tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg
     7021 tttaccatct ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc
     7081 agcattatcc ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc
     7141 tgcaaaataa tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc
     7201 aacagaagct tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac
     7261 aaccttcact ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc
     7321 acctagggta tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt
     7381 tgttgtcttg gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac
     7441 aacccctata acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg
     7501 ccatttttct aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg
     7561 taaataattc gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct
     7621 aagaataaag cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt
     7681 tgattataaa atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt
     7741 gaggttatat tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact
     7801 gtccatacag aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac
     7861 catccttcat gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg
     7921 atcaaatctg cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta
     7981 taaaatatat gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca
     8041 atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct
     8101 tcttttaaat cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc
     8161 tgctcaaggg ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta
     8221 ccatctgttt gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga
     8281 agaaatgccc agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc
     8341 attttgaccc ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc
     8401 gttaatttct tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa
     8461 tcttgcatgc tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc
     8521 accaattaag tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag
     8581 ctattccttc ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg
     8641 ttcaatggct tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac
     8701 caagtctttg atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc
     8761 tagcgattgg tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct
     8821 agcccacaaa aaaagctgtc ctcacagaca g
//
LOCUS       DAJSBN010000085         8718 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJSBN010000085 DAJSBN010000000
VERSION     DAJSBN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.086"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..83
                     /locus_tag="ONT86_002556"
     CDS             <1..83
                     /locus_tag="ONT86_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2353304.1"
                     /translation="SVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            74..859
                     /locus_tag="ONT86_002557"
     CDS             74..859
                     /locus_tag="ONT86_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCR2353305.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            828..1544
                     /locus_tag="ONT86_002558"
     CDS             828..1544
                     /locus_tag="ONT86_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR2353306.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(1646..2680)
                     /locus_tag="ONT86_002559"
     CDS             complement(1646..2680)
                     /locus_tag="ONT86_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCR2353307.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(2684..3742)
                     /locus_tag="ONT86_002560"
     CDS             complement(2684..3742)
                     /locus_tag="ONT86_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCR2353308.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(3962..5110)
                     /gene="nagA"
                     /locus_tag="ONT86_002561"
     CDS             complement(3962..5110)
                     /gene="nagA"
                     /locus_tag="ONT86_002561"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR2353309.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(5186..6022)
                     /locus_tag="ONT86_002562"
     CDS             complement(5186..6022)
                     /locus_tag="ONT86_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2353310.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(6206..7423)
                     /locus_tag="ONT86_002563"
     CDS             complement(6206..7423)
                     /locus_tag="ONT86_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2353311.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(7572..7751)
                     /locus_tag="ONT86_002564"
     CDS             complement(7572..7751)
                     /locus_tag="ONT86_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353312.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            7806..7889
                     /locus_tag="ONT86_002565"
     tRNA            7806..7889
                     /locus_tag="ONT86_002565"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7840..7842,aa:Leu,seq:gag)
     gene            complement(8129..8707)
                     /locus_tag="ONT86_002566"
     CDS             complement(8129..8707)
                     /locus_tag="ONT86_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353313.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         2757 a         1771 c         1462 g         2728 t
ORIGIN      
        1 aatcagtgat aatctatatg acgttttgtt taaagagacg attgagcatc aaacgaccgt
       61 tcggactcaa tccatgtatt taaatgggat agaaactatt tctagcgtta cacctctttt
      121 tagcaatgac cgttttttag gagcagtcgc aactttacga aatcgatcag aagtaatgaa
      181 attaatggag gagcttgggg ggacgaaaca atatataaac tccatgagag ctcaaaccca
      241 tgaattcatg aataaacttc atgtcatttc tggtttgatt gatttgaaga agtatgaaga
      301 agtcacccat tttatccagc agctaaatca aaattataaa aaagagattg gccaagtctc
      361 aagtctgatc aaagtacctg caattgccgg ttttttatta ggcaaaataa acgaagccaa
      421 agaacaatct gttgaaatgc tgttagaaag cgattctttt atccctaaaa tggagatgga
      481 tgactccatc cattcacttt tgcaagtgtt agggaactta cttgataatg caaaagaagc
      541 cgtgctacaa aaacatgaaa gaaacggaca ggtccggatt catttaaact atgacacaga
      601 agctgaagta ttcatcgtga ccgtctgcga tggcggtgtc gggatatcag aagaaatctc
      661 agaaaaaata tttacgctag gtttttcgac gaagggaatc aatcgtgggt ttggattgaa
      721 tctcatccat tcaatcgtca aacgccacgg tggattttta gaattttcca aacaaacaaa
      781 tggcggtaca atcgtctatc tagaattacc aaaaaaggaa gatcaaaatg atgacaaatg
      841 tcctgataat cgaagatgat ccaatggttg cagaattgaa tcgtactttt gtggaaagca
      901 tccctaatat gaacgtaatc gaaagtttca gaaatgtaga agaaggaaaa aaatatttca
      961 gacaaaaaaa cgcagatctg ctgctgcttg atgtatacat gcctggcgaa agtggtatcc
     1021 attttttatc ttggcttaga cagcaaggaa atgatatccc agtgattttg atcactgctg
     1081 cagatgatat cgaaaccata aagaatgtga tccgctacgg tgccttagat tatttgatca
     1141 aaccgttcac tttgcaacgt tttcagctgg cgatcgaaaa gtatcttgct ttctctcaga
     1201 caatccaaac gactgaaaaa gccgatcaga aattgatcga tcaagtattc aaccacacca
     1261 ctcccattca cgaaatacca tcattcatcg atttgcctaa aggtctgtca aaactgactt
     1321 taaaaaaaat aagtgcattt attctaaaag aagatactat tttttcaaca aaagaactgg
     1381 ctcaaaaaag ctcactgtca cgtatatccg tcaagaaata tttgaatttt ttagtagaac
     1441 aagatatatt gaacgaaaga atcaaatatt tggaaatcgg ccagccgcta acctgttatg
     1501 aattgacaaa gaatgggcgg aaaaagctca cagattactg ttgagtcatc gaattatccc
     1561 atacaaaata gtccaagtat acagaagtaa caactgttta cttggactat ttttaaacat
     1621 tttattgacg aatatccatg ttttcttatc ttaattgacg ggctttagga agagatacct
     1681 catttttcgt ttttccagtt tctattattt cacgaaagtt ctcgaaactt gtcgaaatca
     1741 tatttgataa tgcctcatct gtatttgaac ctacatgcgg ggttacaaga actctagggt
     1801 acatttctac taattgctgg atagcagggt ctggaatcgt ctcccatggc ttgaaggatc
     1861 ggaaaaaaac ctctgtttca tttgcaaaaa catcggttgc aaaaccagct aaatgattgc
     1921 ttttcaaagc tcttaaaata gcttcattat cttgaagctc tccacgggct gtattaatca
     1981 agatgctacc ttttttcatt tgagctaaaa atccttcatt gatcatttga tcgttttcac
     2041 ctgggatatg aggtacatga atactgacaa tatcgctttt ggctaataac tcttcaagcg
     2101 agtggaacgt caatactttt ttagcagctt ctgtctgatg aatatcgtag ccgatcactt
     2161 tggctcctag tcccttaaac aaaacagctt ccgtcaaccc tatcctgcct gttcctatga
     2221 tccctactgt gcaatttctg atttcacgac taaacatttg ctcatccact cgaaaatctt
     2281 tgaaagatgt tcggatcgtc gtatacgcag tatttctcaa aagcatcatg gcaaaagtca
     2341 aagataactc tgctatcgca tttggcgaat acgatggtac acgggctact gtcataccaa
     2401 aatcagctgc cgcttgtaga tcgatatgat tgactcccac agtccgtgta aaaacataac
     2461 gaatcccata ttcctgcatt tttgcaagat tggtctcatc tgctatacaa tttcccctca
     2521 acaatacagc atcatggtcc tttgctgaca cgatattgtc atgtgtcaac agttcttcat
     2581 aaagactgag atcataatgg taaatattca atttttcaaa aaaatctatc tcgttcggac
     2641 gaacaccgta acatgcgatt ctcatacttt cttcctccat ttcttatgaa aataaaccta
     2701 tgccatgaat catttgtaat acaacgatcc atacaggtgt cagtaccaca gcaaaaatag
     2761 ttgacagtaa ggatgcattg gaggctaaaa gtgcctccct atcaaaactg atagcatagg
     2821 ctgctgccac tgttgcagct ggtgtagcca tcatgataac gattgttgcc agtgcttcaa
     2881 aattgactgg tagaatattc gttgccgtca aaactgctag aatcacgata ttcaatgcag
     2941 gtacaaatac cactttgact agactgtaat accatgaagt tttttcagat gcagcttttt
     3001 tgaagcttac ttcacctaat gttgcgccga ttgctagcca cgccaatggt gaacttaaac
     3061 cagctaaata atccatcggc tgaaatagcc acggtgcagt ctggtcaatt cgcaaaaacg
     3121 ctacttggct tatctctcct tctgcacctg taactgacac ttgtggcaga tacccttgga
     3181 acagccaaat gaaaaggccg gcaaaagtag cgataatgat tggattgacg aacatcgctt
     3241 tgatattttt agcttccatc ttcaagccac tcatttttat gtatccataa gaatacaaaa
     3301 agattcggta gccaatattg aaaattgagg aatacatcac gccgacagga ccataaatcg
     3361 cactgacgat aggcgttccg aaaaaggtcg tagaaccaaa gatcgtcagg acacgcaacg
     3421 ttgtttgctt atccccttta tagcttaaga aaaaaggttt tgaaactaga ataagaataa
     3481 tataaataac taatccccaa atcaagacat tgattccttg catcagtaat tctgtattga
     3541 tatcttgcat gaaagctcga aatgccagtg ctggcaaagc cactgttaag acgactttcg
     3601 atagaatttt tccaacagat tcagaaaaaa tccccttttt tctacaaaca aaccctaata
     3661 atataataaa aactgttgaa gtgattgcac tgacaatcga catatctgtc aaagtcgatc
     3721 gaaaaatgtc aaataagttc atctttctcc tcctaattat gttcatttat tcacaataaa
     3781 aaaagctttt attttgctaa tacgtattaa catttttaat tttatcttta cattgttact
     3841 tattgaacaa taggaaatta aagaaacttt tgaaacttat gtaaagtgat ttttactaga
     3901 acacaaaaaa accttaaaac caaacttaag aagtttagtt ttaaggttaa cttaaattat
     3961 attatgcgtg gaaacgttcc acgccatcta gataagttgc ttccaatgtc atatcagggt
     4021 tcaatacgat aaagtctgca tcgcgtcctt gagcgatttt tccacatgta tcatcgattt
     4081 tacagctgat tgctggaatc agactagcca tcatgactgc ttgttctggt gttgctaatc
     4141 cccagtctac aacattttta attgcttctt tcaatttcaa tatacttcca gctaagttgc
     4201 cagattctag acgtgctgtt ccttctttta ctactactgg aaattcgcct aaattgtaat
     4261 ttccatcagg catcccaccg gccatcatac agtctgtaat taatgcgaca tgatcatgtc
     4321 ctgctttttc cattaggatt cccgcagcat ttggatgtac atgatggcca tcacaaatca
     4381 attcagaaaa gacatgttct aaagataata acgcacccac catacctggt tcgcgatgat
     4441 tcaatccacg cataccattg aaagcatgga caaatacact tgcgcctgct tcaacagctt
     4501 tttgcgcttc ttctagtgta gcattactgt gtcctagaga aacaactact ccttcttcag
     4561 tcacttgttt gacaaactct tctacacctt gacgttccgg tgctaaagca atttttttga
     4621 ttaaaccacc tgaagcttct tgccattcat ggaaagtatc caaatcagga tccccgaaat
     4681 aacttgggtt ttgtgcccct ttgtgttctt ctgtgaagaa aggaccttca aaatagattc
     4741 ctttgatttt agctcctggt gcttcctgat acattttgcc gattgtctca gctacatctt
     4801 ttaaacgttc tttgcttgac gtcaatgttg ttggtaaaaa tgaagtcact ccacaagata
     4861 acagaccttc agacatcact ttgatgcctt ctgcatcatt atccattaca tcatgattca
     4921 tatagccgtg aatatgtgta tctaccaaac caggagcaat ccatttgccg ctttggtcaa
     4981 tgatgttgac ttctccctca ggtagttctt tggtgtattc accaaaaagt ccatcggtaa
     5041 tttcaagata gcctgcatgt ttgacacctg acgctaaaaa gaatttgtca gcatagataa
     5101 aagttctcat tctattctct ccctttgttt ttctacctct aaagtactct tatttagaga
     5161 agaagtcaag aatggtctac accatttaca aattttttga aatttgtttc acagcttgtg
     5221 ctaagccttc atagaatgct gctctttctg gttcttcttt acgcttgtat attaagcttt
     5281 taagcacaaa taaatcattg aggtaatagt agctattctt ctcttgagcc caatagattc
     5341 cttgattcac ctgttctaac gcttcgtcga tttcattttg atggaataag aaatttccat
     5401 aattataaaa aatttgcgaa aattcacttt ttgatcgttc atcagctgat tgatgcagca
     5461 gttggatgct tcgactcaaa tagtagcgtg cttcagccgt ttttcctgcc acaccatata
     5521 tttttccgat acaactaatc agttgtattt ctgtgtcaga aacatggatt ctttcttttt
     5581 ggctagagta gcttaatgct tctttcaaat aataaaggga ctgagagata ttttgacgca
     5641 acgtgaattc gcaaacaccg agataataat aatagcgttg aaagtcagtt gcttgataca
     5701 gattttcaga aatatcagaa gattttagta cttgttctaa cttccgataa tcacgttttt
     5761 ggaaacaact atccaacagt tcaaataatt tatcgtttgt ccgagtagtt tttcgagaga
     5821 gattcatcac atgatcaatc gttaccccta gccgttgaca aatctgcatc atcaccaata
     5881 cattcggcag ttcctcatta ttttcgatcc gacttaatac actttgtgaa caaatatcat
     5941 gcgaaagcat tttttgcgtc attttttgtt cttttcgaat ctctttaatg atcgttccaa
     6001 aggactcgat taacgtttcc ataagtatcc tccccaaaat atgcgtattc gcattctatt
     6061 cgtattttac tttaggaagt cgcaaacttc aagaaaagac ttgcaactaa ataatatact
     6121 ttagttattt aagaaaactc cactagaaag agagcctcag tgtgtttgtc tgtggccctc
     6181 ctactgaaaa atttggtttt taaaatcatt tttggcgata ggttgctagg aattctctca
     6241 ttttcttagc tgtttgtttc aaatcatcca ttttaggaag ataaacgatt cgaaaatgat
     6301 ctggctggtt ccaattaaat ccaccaccat gtacaagtag aatatgatgt tcatgtaaaa
     6361 agtccaaaac gaatttttca tcatccaaaa tattgaatcg ctttgtatca atttttggga
     6421 atatataaaa agcagctttt ggttttacgg ctgaaagacc aggaatatca ttgattgcat
     6481 tatagatata ttctctttgt tcatagatcc ttccgcctgg caggagcagc tcatcgacac
     6541 tttgataacc gcctagcgcg gtttggataa tttgctgaga caatacattg gaacacaaac
     6601 gcatggagga caacatattc agcccttcta tatagccttt gacgtgtgac ttatctccac
     6661 ttaggaccat ccagcctact cgaaatcctg ctacacgatg agatttggat agaccgttca
     6721 gcgtaacaac gaacaaatca ggcgctaatg tagcaatcgg aatatgttct agaccatcca
     6781 tcactaagcg atcatagatt tcgtcggaat aaataatcag gttattttgt cgagctactt
     6841 caacgatttc ttctaataat tcttttggat aaagtgcccc agtaggatta ttaggattaa
     6901 tgataacaat agcttttgtc cgactcgtta tttttgattt gatatcatca atatcaggat
     6961 accattctgc ctcttcatcg caaatataat gaacaggtgt gcctccagct aatgcaatag
     7021 aagccgtcca caacggatag tccggcattg gaaccagtac ttcatctcca ttgtccagca
     7081 acccctgcat acacatcgtg atcaattcgc tcactccgtt gccggtgtag atatcattga
     7141 tcgtcacatt cggaaaatgt ttcagttggc aatattgctc aattgctttt ctagcagaaa
     7201 agatcccctt ggaatctgag tagccttctg agtcacgggc attcatgatc aaatcacgaa
     7261 tgacttcgtt tggtgcttca aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa
     7321 tactaatgcc ctcttcttgc atccgctccg cttcttctag aacgggaccg cgaacatcgt
     7381 aactgacacc ttctaatttg ttggattttt caaattctct catcacttag ccaatccctc
     7441 atttcaaaaa gttagaactt tccaaatttt ttgttattta ctatttcaat atcatactcc
     7501 atcctagaaa attttgccac ttatttagga aaaacgtaag tattaaaaaa acacaaacaa
     7561 gcgttgagaa cttaattgtc atgatagtca ttccatagat tcgctgccca atccattgca
     7621 attccacaaa tgattcctag cataaaacca agcaaaaaac gtccgaatgc aagtccaatc
     7681 aatacaccta agatcattcc cagtcctgaa aattctccgt catatttctc catcttaata
     7741 agtcctccca tttttgagct tgacaactat gtaaaacctc gctatcatta gtgtgttatt
     7801 attacgcaga tgtgttggaa ttggcagaca agcatgattg agggtcatgt gagcgttagc
     7861 tcgtgtgggt tcgagtccca tcatctgcac tacaactcgt gaagttaatt ttgcttcacg
     7921 agtttttttg tttgaacgtt tctttatgcc attatataca tatcactgta tcctttacaa
     7981 aaagaacgta caatcatgta aacagcctga ggtgagtata tgaaggtcgg aaacaagcta
     8041 ctcgtagaaa cttgcaagtg ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct
     8101 aatactaaag cgagggattt tttgtatatt atctgtttat caccgttatt ataaaggtac
     8161 aaataccaag cacggttaat ccaaattgca taaattcatt tttttgtaca ctcatgcttt
     8221 tcattagctc tttaactgcc attagtacta agatggcgat aacaacataa agtatatagt
     8281 acgtccgcat tccggaagcc catactgtgg gagaaaatcc cataattaac cttgaacaaa
     8341 atcccattat catcaaaata actaaaaaaa tagaagtttt cttattgtca aaacttaaat
     8401 aaattccaac aattaaacat agtaataatg ccaagactac taaagtagct atccaagtac
     8461 ctggataaaa aagtgacagt ttcgttccta actctgtaaa gagattattc aggttactac
     8521 tactccaaat cattgccctt ttatttccat tgacgtaagt aaagctttga cccattgtat
     8581 taccaaaata aataatcagg tttaagaaaa acggtaatgc agtcaatata cgcacataat
     8641 agttttgaca tttcctatat gttagaagga aaattaagaa aaatagcaat aaaaataaga
     8701 tattcatgtc taaaaaaa
//
LOCUS       DAJSBN010000086         8694 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJSBN010000086 DAJSBN010000000
VERSION     DAJSBN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.087"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..777
                     /gene="menA"
                     /locus_tag="ONT86_002567"
     CDS             <1..777
                     /gene="menA"
                     /locus_tag="ONT86_002567"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR2353314.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1712..3058)
                     /gene="gor"
                     /locus_tag="ONT86_002568"
     CDS             complement(1712..3058)
                     /gene="gor"
                     /locus_tag="ONT86_002568"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR2353315.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3192..4445)
                     /locus_tag="ONT86_002569"
     CDS             complement(3192..4445)
                     /locus_tag="ONT86_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR2353316.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4451..5374)
                     /locus_tag="ONT86_002570"
     CDS             complement(4451..5374)
                     /locus_tag="ONT86_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR2353317.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5595..8087)
                     /locus_tag="ONT86_002571"
     CDS             complement(5595..8087)
                     /locus_tag="ONT86_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCR2353318.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8094..8561)
                     /locus_tag="ONT86_002572"
     CDS             complement(8094..8561)
                     /locus_tag="ONT86_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCR2353319.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2393 a         1831 c         1446 g         3024 t
ORIGIN      
        1 aattacatgg attatgaaaa ggctcattct gaagtatata agtatgaaga aaatgtcatt
       61 ggccgcagaa atctatctcc tgtattgatt cgaaacatga ttttagctat gattgcattt
      121 gtttttgtta tcggcgttat tctaacgatc aatacgggtt ggcttttact tttgatggga
      181 tttatctgtt gttttattgg agtattctat acattcggac cgattccttt atctcgaatg
      241 cctttagggg aaattttcag tggggtgact atgggccttg gaatttttgc catggtcgtt
      301 tatgtcaata cattcggttc agatgtctat gatctgacag tcaacttttc atctggacaa
      361 tttgatttgg taggaaatct ttgggctata gcagcaatcg tattagcttc cttacccttg
      421 gtgtttacga ttgccaatat catgttggct aataatttgc gggatcttga ccgtgatatt
      481 gaaaatcatc gctatacact agttttttat atcggacgtc ctgcgggaac agtcttattc
      541 cagctattga tgtatgcttg ctatttcgtg attttaatgg ggttgttgag cggtatctat
      601 gaatggccga tcttggtcac cttcttgacc ttgccaattg tgaagaagca tttggatcaa
      661 ttcaagcaaa cgttacccca gcctatcagt ttcagctatt ccatcaaaaa tatggtctta
      721 ttcaatgcca gttatgtatt ggggctatta ctaacaatca ttatgcaagc catttaacag
      781 catgaagagg ttgtgaaaaa actgccctgc atcaagtaat aagaacagcc aaataaaaat
      841 agctccttat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
      901 gtttatcagg aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
      961 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
     1021 ccgtttatca gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
     1081 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     1141 accgtttatc agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga
     1201 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
     1261 actatttgtt cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc
     1321 acttgatttg attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg
     1381 gtattctttt aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt
     1441 taaacatctg ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa
     1501 cttaagagtg cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa
     1561 tctccaactt ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac
     1621 taaaaaacaa aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca
     1681 ataaaaagct gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc
     1741 ctgttggatg gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag
     1801 ccacggcaaa accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga
     1861 ttctttcttc ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta
     1921 atgcaaagta cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt
     1981 tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga
     2041 ctgtcggaac aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg
     2101 ataaacgtct accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac
     2161 caacagcaaa aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt
     2221 ttttgtccaa ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc
     2281 caccggcaaa aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat
     2341 attcgtcatt gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag
     2401 cttcttggta catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc
     2461 gttcttttcg atatgcccac atgacttcac tacctaaacc acgaaataca cctgaaagtt
     2521 cagccgcaat gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa
     2581 aaaatccatt tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc
     2641 ctccagtagc gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt
     2701 gactatcgat aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc
     2761 tttgataagc accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga
     2821 aatcaacatt ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt
     2881 ccaaaatcgt acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg
     2941 ttccgcccaa ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt
     3001 ttgctgaagc aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt
     3061 tgttcctcct tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga
     3121 aaacaagtgt aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat
     3181 cagctttttc cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact
     3241 ggcatagtta aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta
     3301 tgcataactt caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca
     3361 tctcctacag aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa
     3421 gataatactt caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt
     3481 tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc
     3541 cataactcgt cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg
     3601 tctacagccg ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt
     3661 ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt
     3721 gacatatcca ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg
     3781 tttcgttctt gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa
     3841 catccgccac ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa
     3901 taagagatac acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga
     3961 atctctgcca cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct
     4021 tctttccaga attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc
     4081 ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact
     4141 gcagaaatac cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct
     4201 tcatgggtga ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca
     4261 actccttcag ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca
     4321 ttcccgccaa tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt
     4381 gttcctgctg gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc
     4441 atcatactcc ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac
     4501 ggttttcagg atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc
     4561 agctaccttc taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt
     4621 tttcgcctgg agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt
     4681 cattcaattc attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat
     4741 cttcataaat agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt
     4801 cacgatcttt ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt
     4861 tggattgatg gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt
     4921 cttccatcgc cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc
     4981 cttttttcgc ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa
     5041 tatacggtat acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa
     5101 ctgtgatcac atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca
     5161 ttttttcagg atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg
     5221 ctaattctac caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa
     5281 attgctcttc accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt
     5341 caatagtttc tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa
     5401 tagtagcata gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa
     5461 attatttcac aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat
     5521 tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta
     5581 cacaggccaa gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat
     5641 gtgatcttac cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca
     5701 agcaaagcct cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt
     5761 gcaccatatt caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt
     5821 ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg
     5881 attgtatgga tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc
     5941 aagaattcag gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg
     6001 tgatctttag taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc
     6061 gctccgatat ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta
     6121 gaatctgtta aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt
     6181 gctttctcca cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc
     6241 aactgtccac cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta
     6301 ctgtattttt ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac
     6361 atgacttctg aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac
     6421 atgaatgaac caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca
     6481 cgtgaaactg ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc
     6541 agttctaaca agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac
     6601 tccgatacaa ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt
     6661 tttgcttcaa ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt
     6721 aattgagcag cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt
     6781 tcatcgattt ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg
     6841 actttcgctg ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg
     6901 atataacgaa cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca
     6961 tggtgctctt catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt
     7021 ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat
     7081 ttttgatatt catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc
     7141 ggtttcaaga tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta
     7201 tgcaattcat caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt
     7261 ttcattcgat cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca
     7321 agcatcatca ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga
     7381 gctaaccctt cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta
     7441 ttctttgtcc gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca
     7501 actgggtcca gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc
     7561 gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc
     7621 attccttttc cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct
     7681 tttgaaaggc tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt
     7741 aataagctca tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg
     7801 ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta
     7861 gggtaagaac ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt
     7921 tcattcaaat ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag
     7981 gctagtaaaa catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt
     8041 gcaatttcta atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc
     8101 ccatcttcat aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct
     8161 tcatattcat tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct
     8221 ttttttgtaa ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca
     8281 ctgattgtat catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc
     8341 cttacttttg caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca
     8401 cgttggatgg taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag
     8461 aggtctgcca tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt
     8521 atatatgatt caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac
     8581 taatattgac cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt
     8641 aaataaatcc tttagaatca acaaaaaaaa atacggaata tactattccg tatc
//
LOCUS       DAJSBN010000087         8489 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJSBN010000087 DAJSBN010000000
VERSION     DAJSBN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.088"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..173)
                     /locus_tag="ONT86_002573"
     CDS             complement(<1..173)
                     /locus_tag="ONT86_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2353320.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="ONT86_002574"
     CDS             complement(593..1714)
                     /locus_tag="ONT86_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR2353321.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ONT86_002575"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ONT86_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR2353322.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ONT86_002576"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ONT86_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HCR2353323.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ONT86_002577"
     CDS             complement(4304..5215)
                     /locus_tag="ONT86_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2353324.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ONT86_002578"
     CDS             complement(5232..6239)
                     /locus_tag="ONT86_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR2353325.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ONT86_002579"
     CDS             complement(6236..8365)
                     /locus_tag="ONT86_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2353326.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8489)
                     /locus_tag="ONT86_002580"
     CDS             complement(8370..>8489)
                     /locus_tag="ONT86_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2353327.1"
                     /translation="GQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1485 g         2677 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgacc
//
LOCUS       DAJSBN010000088         8330 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJSBN010000088 DAJSBN010000000
VERSION     DAJSBN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.089"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(63..1022)
                     /locus_tag="ONT86_002581"
     CDS             complement(63..1022)
                     /locus_tag="ONT86_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2353328.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            1118..1663
                     /locus_tag="ONT86_002582"
                     /pseudo
     CDS             1118..1663
                     /locus_tag="ONT86_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1846..4521
                     /locus_tag="ONT86_002583"
     CDS             1846..4521
                     /locus_tag="ONT86_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCR2353329.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            4703..4942
                     /locus_tag="ONT86_002584"
     CDS             4703..4942
                     /locus_tag="ONT86_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353330.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            4958..6331
                     /locus_tag="ONT86_002585"
     CDS             4958..6331
                     /locus_tag="ONT86_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2353331.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            6335..7969
                     /locus_tag="ONT86_002586"
     CDS             6335..7969
                     /locus_tag="ONT86_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2353332.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            8132..>8330
                     /locus_tag="ONT86_002587"
     CDS             8132..>8330
                     /locus_tag="ONT86_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353333.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2876 a         1338 c         1644 g         2472 t
ORIGIN      
        1 aatgaaaatc acctttataa actccattct tttccactgt tttattaata ttttctgatt
       61 tgtcaaatta agctagacaa tatgtcttta tctatgcagc tcaaaaatac ttccaatggt
      121 gttcgataat ttaatgattt tcttggaata tggtttcttt tcgaggcaac ggattgaata
      181 aaggtttcat ccgtgtccct aaagtccatt gttttaggta agccatctcg tctaagcaag
      241 ccgtttgagt gctcatttag ccctctttgc gaaggtgttc ctggatctgc gaaaaagatg
      301 gcaatatcgt tcgagttact tatagatcgc cagttggaaa attcttttcc acaatcaaat
      361 gtcatggatt taaacaagtg tttaggcatg gatttgaacc aatcatttaa cctgttttcg
      421 atatcttcgg ctcgtctgcc tgtcggtttc aaagtaataa taaccttgga taaacactca
      481 accaatgtaa taacagcgct cttgtgttta actcccacaa tggtgtcgac ttcgagatga
      541 ccaaattctt tagtcaggtg tggatagaga ctgttccgtt catgaatcgt tctgcgaaag
      601 gcctgttttc cacgcttttc ttgatgatta ttcggtttac gtttcccctt cataggtaac
      661 gactgtgtgt caaaaaggcc ttttttaaac atccgataga gtgtacgaac agaacaatca
      721 attggaaact cgccacggcc gataatcaca tcgggtgacc atccttgagc taccttttct
      781 tgaacatatg ttgattgttc atcagataaa acggttgaac gtcgtccaca acgagattta
      841 ttttgtttgt attgacggta ataatctagc actttttttc cttgtttgag aaacgaataa
      901 acattataaa tggtttgtct agagcgattt aaactttgtg cgacaagagc gacaggtttc
      961 tcgatattga aataagactc tatgattact agttcgtctg ttgtaagatg ggtgtaagtc
     1021 atttgtactc actccttatt aattctttgg tcggaactat tttgagtgta gcacaaatga
     1081 tctttttagt tgtctagctt aattttacaa tcgacgtttt caaaatgctg agactattga
     1141 acgagaacat cattatttcg atttattagt gaatacagtg gtagaattag cgaaatgggc
     1201 aaagtcaaac gaaacttata gtcaagtact taaaagaatt atgagtcaag aaacttcatt
     1261 tatctatgag ttaagcaagt caaatttgaa atatacttat aatgaagatg aacttgaaat
     1321 tttagataat ccagaaaatt cacaatggat taaagagttg aagagagaag actttattga
     1381 gacactaaga attctaaaat cgttatttga tttgaaatta gccaaagcta ccatcatggg
     1441 aattgaaaga gattcatatt attgggagga aattggacta tatgctcccc agattgaagg
     1501 tgaagataaa gagaaaagga tagacaacat tattaactat attaatagtt taagtgaacc
     1561 tttaaaatgt agaggacttg attttatagg aacattagga tggacagaaa catactatga
     1621 gaaaatatat aaagaaataa ttgaacaata ttttcctaaa taatttaata aggattgatt
     1681 tatcgtaaaa gaaaaaagtt gtgatttcca aaatcacaac ttttttcttt tgtcaagatt
     1741 ttgagtggga tatatttttg tagaactgaa gatcatgaaa ttatgagagg ttggaggtaa
     1801 tgtagtcata caaataaaaa ttttttagaa agtgagtggt aattaatgaa aatgaatcac
     1861 acttcaattc agaagcattt acaactgtta aatacactgg ggatttctta tgttaatttg
     1921 agaacttttt ctacattaaa caagaaaacc gtttctcata ttttttctac tgcagattct
     1981 caagcaatta ttaactattt agaaaaacag aaattgtctg aaggtacagt aaacataact
     2041 tataatccta ttaaacaaga ggtgattaca caaaaaaagc attctgtgag agacaatgat
     2101 attgaaaaaa ttcggtttgt atttattgat attgacccta aaaggaaaga gggaagtgca
     2161 acagatagcg aaaagaaaaa ggcagaaaat gttatggaac aagtagaacg ttatttgaaa
     2221 gaaaagagaa tagaatcatt tgtaaaagtt gatagtggga atggctacca tatttttctt
     2281 cccataaatg aacaaccaaa taatcacgag acgattctta ctattcagaa ttttcttcaa
     2341 ttacttcacc gaagatttgg agtagaagat gaagttgata ttgatacttc tgtttataat
     2401 ccttcaagat tatgcaaatt agttggtaca ccagcggtca aaggaaagtc cacaaaagag
     2461 cgtccgcatc gtttatctag tttcctttct attcccgata acatagatag aagtaattgt
     2521 tttaaaattc ttgaacagat agttgaagaa aattccatga tgtcaacctc tagagtaaaa
     2581 agccatccat cttttagaga agtgttacct tttattcaag cagactataa acaatggtta
     2641 gctcactatc ctgaattaac ttataccata aaagagggcg atcataaagg agttacctta
     2701 cttatatttg acgaatgtcc attaagaaaa cacacgaata attctaatgg ttcttgtttg
     2761 tctgttacgg ctgataataa gattcgattt cattgtctgc atgcttcgga taaaaataaa
     2821 gatattcaag actttgtaaa aaaatatccg ttgccaaaac ctgtaaatcc ttctgtagaa
     2881 tcattagaat ccaatcaaga atattatttt gaccagttta aactgaatca agaaggagta
     2941 tttttggtga aagaaggtca agaagaaaaa gttgcttgtc cgatgtttat taagcaaata
     3001 cgcaaaaata tcgaacaaaa agtcattaca cttgttcttg cctattatgt cgatcattgt
     3061 tgggatgaaa tagaaataga aggtggatgt ttacaaacta ataattttaa acaattagca
     3121 aaacatggct taatcattaa agctagaaga gaagctgaag tcgttgactt tttacagatt
     3181 caacgggaga atgctccaat cttttttgaa catgaatctc ttggctggaa aaaagaaaat
     3241 gaagcaatga tttttcagtt acaagattct tttggacgtg aaattccttc tgtattaact
     3301 aaaaactcat gctaccaatt aacaactaat ggtacagaag cggaatttga cgcattggtt
     3361 cataagtatg ttttaggaac gaacttagaa cttgcttggt cgattggtat tgcttctatt
     3421 gtgttaggct atttaaacgt tacgaaggct aatacttttt tgagtttact aattaaccta
     3481 cagggcaaga gcacaacggg taaaaccact acgttacact ttattgcctc tttatttgga
     3541 aatccaacta cggttcttag aaatatgaat gctaccaata aagccattat aaagctagca
     3601 tcaaacaatc agggaggtgt tccactaata ctagatgagt taggagcagc aactcaaaat
     3661 aactttagct cgttgatcta tcaattagct tcaggagaag aaaggctaag attagattct
     3721 aattgtcagc ttatgccaca agagactttt tctgttttga cccttacctc ttctgaagag
     3781 cgtttggaaa attatttcaa tgagaaatta actggtttga aagttcgata tatcgaattt
     3841 tctgatatca agtttacaaa ggatgcgaat cacgcagaga aagttaaaag tctttcttct
     3901 aatacttttg gttggggtat aaagttattt cttttgaaac tctttgaaca agggattaca
     3961 aaaattgaag caacttttgt cgaagctcaa aaaaaactat gggctgtttt acctgaacat
     4021 tctttgaagc aacgcattgg taataatcta gctttgattt atagtggagc cctcctaatt
     4081 caagatatac tgggctatcc tttacgatta gagcatatac agacggtgct tattaattcg
     4141 tacgaacagt taattgtaga tactagttta ggaagtgagc aaacggtatt tgaccgagtg
     4201 aaaacattgt tggtgatgaa tagccataaa tttttaacaa acagtgacaa caaacatacg
     4261 cccagtacga tttggggaaa agtatcaatt caaaaaaatg gggatattaa ggtaaatatt
     4321 tttcctatcc cgtttgagga cttgttaagg cgagaatttc aagtaaagga tctaacctat
     4381 ctttttaggc aattgcttga ttcgggtaat atgtgtgcag aaaaagggag aagaacgaaa
     4441 cgtattcaca tgaatcgtca agttcttcca acatacgaga ttctattacc ttcttcactc
     4501 aaatcgtatt ttcgattgta atttagaata aaccaaagta tgaaagtgag gcaccatgtt
     4561 atggaaatat ttcttattga tttagcaaaa caactgatta cttatctgta tgatagcgag
     4621 caaacccaaa cggaagtaaa tgaagcttga tctatgcttc atttacttca ttcaatcaaa
     4681 catgaaagaa aggaaaattc taatgactaa aaataaaatg acactaaaag cagaggttct
     4741 tctgtatatt caagaacatt tttcaaatca agcctttttt acaaaaccaa tttacttgga
     4801 tttcgaaata agaggggtat ctgctggatc tatcgggggt actcttcaag cgttgaagaa
     4861 cgaaggatat ttagaaaatc actttgtgca acgttcgttc aatagaagag tcgtgaaaaa
     4921 atggtattta gttcattcat agagagggga atcaagcatg cgtgtggctt gttatgatcg
     4981 aacgtctgtt tatgaagaag cgaaaattag taatgacgct caatatgatg cagtgaaaga
     5041 gtatgtttta gagaaatatg aacaaattcc ccaacgattt tcagacaaag gagaatcagg
     5101 ggaaaaagcg gagcgtccag cttttcgaca gcttagagct tgggtttgtg cgtcagaaga
     5161 agagggggtg atctatgttt ggcgttatga tcgattggca cgtcaagcca aaatagcctt
     5221 agaatttatt gagttgtgcc aacaacatca ggttcaagtg tattcgattt ctgaacctct
     5281 tcctgcttct ttctcacacg cgtcaacgaa taaactattt gtgcaattat tgttcacttt
     5341 tgcagaacaa caacgaaacg tcattattga gaatgttcgt gccggacttt cctataaaaa
     5401 gcaacaacgg caatatttgt cttctgcggt accatatggg tatcgtttag tgaatgggaa
     5461 aatccaacaa gaagtacacg aagcaaggat tgttcgctat atttttcaac tttatttgac
     5521 caaaaaatat ggctacaaaa agctttgtca acgattgacg caacaaaagc ttttcttccg
     5581 cgaacgtcct tttcagccct atcatattta tagtatccta aaaaatccat tgtactatgg
     5641 cgaaatcaaa ggaggatctt tggggaagta tctaggaacc tttgaaccga ttctctcgaa
     5701 agcaaccttt ttacaagccc aagagattcg ccaatcaagg tgtacagcta aaaaagatac
     5761 gtatctgtac ttattacgtc aaaaaattcg gtgtcccttc tgtggtcgtc atctgagttc
     5821 gaagtatcaa tggaatacaa agaagacgaa gacgcttcat tattatcatt gtaccgaccg
     5881 aagctgccaa ggaatttttc ttcgggcaga aagggttgag tcagaagtat tggaaaagat
     5941 acaggctttt tttcaatacg aaggcgtcta tcaagccttg attgcccaat taacgcaaca
     6001 attagccgaa aagcgaaaag atgaaaaacg caaagtaatc caacaaaaga aacaagaagc
     6061 acaatggatt aaggcatttg aagagggcaa ttgttcactg gaagaactca agcaacaact
     6121 gcaagctctt caaaagccga tgtctacttc ttcgtttctt tcactccaac agtgtgaagc
     6181 aaaagtgaaa gaatggcttc aactaaaaga cctctctagt cgacaattat tatgtgacta
     6241 tgtttgtcgg attgaggtaa caaaggataa aacagtttgt gggatttact tccgacaact
     6301 gacacaaaat attttagaaa ggaacgaatg atctatggaa atgatacaaa ccaaacgagt
     6361 cgcattgtat attcgcgtga gtacacaaga acaagcttta gaaggacata gtattcaagc
     6421 acaagaacaa aatggacgac agtttgccga acggatgggc tatgaagtgg tagaagttta
     6481 tgccgatgaa ggaatcagtg ggaaatccac aaaaaatcgg ttcgcctatc aacgaatgat
     6541 gaacgatgct cggaaccatc gttttgatct actagtgatt tggaaattga cccgtctcgg
     6601 acgcaatatg ttagatgtct tacaaacagt cgaggaatta ttgcgatatg acgtaggatt
     6661 acattcgatc tcggaacaat ttgacgtgac cacttcgaca ggtaaattga tgttacaact
     6721 ccttgggagt ttcggtgaat ttgaacgcaa ccaaattgcg gagaatgttc aaatgtcaat
     6781 gcaaagctta gtgaaagata aaaagcgtta tgcaggaggc cgacgtttag gctatgtcag
     6841 tggtattgac acgaatggtc aaaaacaatt ggtcattgaa ccagaagagg cgaaaattgt
     6901 tcgtctgatc tatgcaaaat atttaggcgg agcagggtat cgagcgattg ccaatttttt
     6961 gaatcgtcaa ggatatcaaa cagtcaaaaa gaatgctttt tctaccacgg cagtcaaaga
     7021 tatcttacgc aataaaattt atgcagggta tttagaatat gcgcgctaca tcaattggga
     7081 aaccaaacga cggaagggga agaatccgag accgatttta gtgaaaggaa cccatgaacc
     7141 gattatttcg gaagaagatt atcaaaaagt acaagaacgc cttgctttag aaggaaaaca
     7201 acccaaatgg aaccatacag gtgaaaatgt attgacaggc ttgttacgtt gtccagaatg
     7261 tggcgcaccg atggcagcga gtaatgtcac aaatacccta aaggatggca caaagaaacg
     7321 gattcgatat tattcttgtt cagtctttcg gaataaagga gcaagtgtgt gtcatgccaa
     7381 ttcgattcgt gccgaacaag cggaaacatt tgtcgccgac cgattaaagg agatcgttca
     7441 gctaccagaa gttcttcccc gattggtggc agcgttaaat gaagaaatag tgcgacaaag
     7501 ccaaccattg gaacaagaac tcgtggtgtt actggaacga aaggaagaat tgaagactaa
     7561 aattgaaaaa tgggaagctg ctttggaaga tagtcctgaa ttgtttccga tgttaaaaga
     7621 ccgcttagac gaattgactg aaaaacgacg acagttgcac attcgagaaa atgaaatctt
     7681 agggatcttt caacaaggag agccgattca agtcaaagat gtccaacgga tattgactaa
     7741 tttggatcgt tttcttgccc agagtgagaa gaagcaaatc aaagccttgt atcgtacctt
     7801 tatagagaaa ataacctttg atcctaacca taaggagatg ttggaaatta cgatgaaatt
     7861 cacgcatgcc gtggtacaac aattgaatga gcaatatcaa atagccgtat ccaaaacaaa
     7921 ggatacggct atttttgtgt taaaaacacc ctttacgtta acgatctagt ctaggttata
     7981 tcaaacaacg ttagaggggc gctaacgctt ctctagcgat ttgaaatcgt tagagaagta
     8041 gttagggagg tccttatcat aaatagggat atttattaat gaatatttga aatataattg
     8101 tatataagaa ttaaaaagag tggggataga tatgaaaaag atgggtaagt gtattttatg
     8161 tgaaaaatac actgaactta ctaaagaaca tgtaccacct aaaaaatcag ggaatactgg
     8221 aggtaaaaag accagaactg gtaatttaga tgatttcctt aagagcgatt ttacaaaagg
     8281 tgattttccg aaggggatta aaaggaaacc tcaaggaaat gtttattata
//
LOCUS       DAJSBN010000089         8108 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJSBN010000089 DAJSBN010000000
VERSION     DAJSBN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.090"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1065)
                     /locus_tag="ONT86_002588"
     CDS             complement(<1..1065)
                     /locus_tag="ONT86_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCR2353334.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILE"
     gene            complement(1065..2201)
                     /locus_tag="ONT86_002589"
     CDS             complement(1065..2201)
                     /locus_tag="ONT86_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR2353335.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(2233..3684)
                     /locus_tag="ONT86_002590"
     CDS             complement(2233..3684)
                     /locus_tag="ONT86_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR2353336.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3728..4492)
                     /locus_tag="ONT86_002591"
     CDS             complement(3728..4492)
                     /locus_tag="ONT86_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCR2353337.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4520..5218)
                     /locus_tag="ONT86_002592"
     CDS             complement(4520..5218)
                     /locus_tag="ONT86_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HCR2353338.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5230..6009)
                     /locus_tag="ONT86_002593"
     CDS             complement(5230..6009)
                     /locus_tag="ONT86_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCR2353339.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6025..6969)
                     /locus_tag="ONT86_002594"
     CDS             complement(6025..6969)
                     /locus_tag="ONT86_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR2353340.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7015..7794)
                     /locus_tag="ONT86_002595"
     CDS             complement(7015..7794)
                     /locus_tag="ONT86_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353341.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2308 a         1699 c         1223 g         2878 t
ORIGIN      
        1 ttctaagatt ttgtatgctg tttgaatatt ataatctgcc tttattaaaa tcgaacgttc
       61 tgaatctcga tgagagatat ctacatttgc aatctctctc gcccattctt ttgccgattg
      121 atttaaactt atgaaattgg tcttatcact gaaagcaact tctgtaggaa ctttctccga
      181 aaagaaacac ggtaaatctg caaattgagc ttcaataccc actaacggaa ccccttcaaa
      241 taatgatggc aacattaata cgtcaaatgc ttgataaaat ttttctatat catttctatt
      301 tcctaaaaat ttaatatcat catctatgcc acttttcttt gcataatttt ttatttcttc
      361 ttccatatca ccagttccaa taattaataa tacggaatta ttcttaatag cgtgaaattc
      421 tttaaaaatt tcaatcaaaa aacgatgatt tttagaatat gaaattcttc caatatgtcc
      481 cattacaaac gtctgacttg aaattcctaa tttcattcgt acatcatttc taatactttc
      541 atcataataa aattttttag catccactgc atttggaatc aaagtgaatt tatcatttcg
      601 aaacaaaaaa tcacctgcta cttttccaca tgaaaattca tgtgtcaaaa cagacgttaa
      661 cttacatcga taatattgct ttaaaagata tttaaaatct ctatctatcg atgtattatg
      721 gctatgtgct atccgtatcg gaatcccagc tttttttgca gcttttaaag gtaaataact
      781 catcgcatca atatgagaat gaacaatctc atattctgga tgatccttaa aaaattgttc
      841 catccattta aaatatttag gataattttg tggataaagt ctcggtgcat aatagacctt
      901 tcctccaaga cttattattt cctcatcata atcactttta tatggacgat gtgtcaaaaa
      961 atcaaactga attttagttc gatctatatt cctataatag ttcattagca tcgtctccaa
     1021 ccctgcacga tgcatatcat tcacacactg taatattcga atcattatat atttccccta
     1081 tacaaattta atgtttcttg tacgatttta tcccaattat atttactaca aataaactca
     1141 gtagcttgat tcttcaaaac tttaaccatt tccgattgat tgcatgcctc ttctaatctt
     1201 atctttaaat cacttacatc cgatttctta aaaatcatag ccttgtcttc tactacttcc
     1261 gtacactctg agatatttga aactatacaa cagttcccat aactcattgc ttctaacaaa
     1321 cttaatggca ttccttctaa atcacttgga agtgtataga tatacgcatt actatataat
     1381 tcttctaatt cctgtccttg tacaaatcca gtaaatataa ttcgctcatc accttttgct
     1441 aattccttta gttcatttgc aaattcatct gtatcagagc ttcctccggc tattataagt
     1501 tttttatctg tctgaacatc tttaaatgcc tcaattagat accgaattcc tttttcggga
     1561 accaatcgcc ccaaaaacaa aatataagaa tcttttgtta atccatactt ttctgtaatc
     1621 aatcctgctg attttatttc aattttttta acgccatttg gtataaacct tgtttttctt
     1681 ccatatatat cttcaaaata attttggacg ctttcactaa gtacaattat ttcatctgca
     1741 aattttacag cacacttttc ccctaacatg atgtatgtac tagcaagctt gttccatttt
     1801 tctctttgat gatctagacc atgaattgtt gcaacacatc ttttcccaaa aagttttggc
     1861 aaccacaaca tagcacatgg tccttctgca tgaaaatgaa caacatcata ttttccgaat
     1921 gctgctctaa ttccaccaaa tactgaagaa ctcattgctg caattccttt tatatttaat
     1981 gtaaaaatag tctttaattt tacaccttta tattctttat ttttatgctg gtcaaattcc
     2041 tttccgctaa catgatgacc acttcgatta tagcaagtca cttcatgccc aagtgcaacc
     2101 attcgcgttg ccaattcttc taccactatc tcaattccac cttctcttga tggaattctt
     2161 ttatgaccta gcatacagat ttttaatttt tttcttgcca tcacgtttta ctccttctat
     2221 agtatcttta tcttacatgg aaccgtttct atgaagcaca gttttgactg tcctaaacaa
     2281 aattctaaaa tctaatccca tactccaatt cctgatatat tctgcatcaa gttttacaac
     2341 ctcttcgaag tctgtgatat cacttctacc actcacctgc cacattccag taatcccagg
     2401 tttcatcgca attctcgcac gatgatgaaa atcatacttc tcatattctg acttcaagca
     2461 aggtctaaac cccaccaggc tcatttttcc cattaggaca ttataaaatt gtggaaactc
     2521 atccaatgat gtagatcgaa taaattgtcc aattcctgtc ttctttgttc catcaggaag
     2581 tattttattg ccaatgattc ttgggtcaaa atccatctta aacatgagat tattactcat
     2641 cttattttct ttcatcaact ctgctttgcg ttcttctgca tccatataca tactacggaa
     2701 cttatacatc ttaaatgtct taccattttg accaatacgt tcttgtgcaa agaaaatcgg
     2761 ccctggtgat ttgatgtaaa taactggtgc tacgaagata aaaataacac ctgttgcaat
     2821 acatccaata attccagcaa gaatgtcaat cgttctcttt ataaatgctt gcctcatcgt
     2881 cataacatta atactagatg taataacagt ataatcacca actcggccaa taaattgttt
     2941 tccacgtgta ctatcagaca cttttgtaag attcatatgt acagcaagtc ccatttccga
     3001 acatctggcg attaattctt tgggatatgg aacactttcg tcaacattga tgaatacttc
     3061 atctacccaa ttctgtaaaa gatattccgc tgcattatca gcatttgcaa ctactgggac
     3121 acctccgatg gtagttccat acatatctct gtctactaca atcacaccac taagtttata
     3181 catgccataa ttcttctctt taagattacg aactacattt tttgcaatct tttgagatgt
     3241 tacaataact aacgatacat cttcaccgtt tttcatgcgg tgatgcaaca accctttcca
     3301 aagtatacga attatatatg ttaataatcc atacaccacc cccattataa ataaagaaac
     3361 tctggaataa tcatttccat cctgcattgt aataaggtat aaactgcaca caagtaacag
     3421 cataaaagat tgctttatag aagctacagc ctctttataa tatcctcttt ttaaaacatt
     3481 tttatatgcc tcaaataaaa atataatcgt aagattctca agttctataa aaattgccat
     3541 gtggcgataa ataggaatca catacggatt ccagtcaccc tgtcgcatta catatgatat
     3601 ataaaaagca agctggacac aaaataagtc taataaaata aaatcaatat gtttagacca
     3661 accacttgag ttttttctat acatctctca ctctctcctc atttcctttt tatctacgaa
     3721 taaagaacta aaataaacca aacttcttct tttttacctc ttcatagatt ggataattga
     3781 tctcatcccc gttgatcaga tcctttgcga cttgtttcat ctgtactact ttttccttac
     3841 caaactcctt ggcaatctgc tcatagcact cttttaagta aaaggtgcgc ttagagaccc
     3901 cgtgtgcatc agaagcagcc atctgcacga gattatgttt gaccattgtt ttggctgttt
     3961 tctggatatc tttgccaaat acaccaacat aacttggcgc agtaagttgg gccaaacatc
     4021 ccatatctag gaacggaagc aaatgatttg ggtcttctcg aaaatgcgcg ttgcgttctg
     4081 gatggacgat cactggtatc tttcctagtt ctaacaactc aaaaaaaagc cgttctgcat
     4141 ataacggcac ttccagtgtc ggaaattcga tcagaagata tgtatcatct aaatctgtaa
     4201 ataaaatttc atctcggtgg atgtcttcga tgagcgttcc ggtaatacgt acttcttgtc
     4261 cttccaataa cgtcaaaggc aactgtcttt tctctaattc ggcttgtaac gaagcgacta
     4321 gagaaatgac ttgtgatttt tcattgctgt attttccatt attgtggtgt ggtgtgcata
     4381 aaatatgcgt aatgccttgc tgaatcgctt tttcagccat tgcaatactt gcttctaagt
     4441 tttctgcccc gtcatcgatt cctggtagga tatggcaatg tagatcgatc atttctcttg
     4501 tctcctctct tcttattcct taatttccgt agtaataacc agcatctttg ctgtgttctg
     4561 ctccgttgta tacgacacct aagactcgtg cttgtaccat atctaacagt tcttttgctt
     4621 tagtcaatga ttcttttcgt gacgtatttt cccgtacgac caataaggtg ccatccgttt
     4681 ttgaagacat gatctgtgca tctgttacgg caacgactgg cggcatgtcg aagatcacta
     4741 catcaaatag ttgtcttgct tctgctaaaa tctgatccat tcttggcgaa cttagtagtt
     4801 ctgatggatt tggtggttta ggtccacttg gcagaatcga caaattgtcg actggtgttc
     4861 gttggatctc atctgctaca cttcctgatg aactaagaac agtacttaat ccgcttgcgt
     4921 tgttcaactt gaacgtttta tagatgacgg gcttacgtaa gtctgcatcg actagcagca
     4981 cccgttgacc agatttggca aaaacaacgg caatgtttgc tgcagtggtc gatttccctt
     5041 ctccaggtcc tgatgaagtc acaacaatcg ttttgatttg ctgaccagct gcggaggcaa
     5101 actggatatt cgtccgaatc gtacgatatt gttcagcgat tggtgaagag ggattggtga
     5161 gtgtgaccaa actgactgca tgagtttggg ctgctttttg tttctgtgtt cgtgccattt
     5221 tctttcctcc tagactcttg agcggcttct gcgtgatggc gctgttttat cgtttgtagc
     5281 ttttggttgt gtctgagctg gtcctttttt acggatacca gcattcaatt ctttcgctga
     5341 catttctgga actgttccta agattgggaa tcctaatgta tctgtcacat atttttcatc
     5401 tttcactgtc cgatcgagta attccaataa gaatgccaga cctacaccta ccatcattcc
     5461 taacattaag ccagtcatta aattcaattt attatttggc gaaacaggtg tcgtattggc
     5521 aactgcacct gaaatgatcg agattttatc aacattcatc acatcttttg cgttctcttg
     5581 gaaaacttgt gaagttgtgt tagcgatctg ttgagcagcc actgcattcg tacttattgc
     5641 ctgaatcgag aacatttgtg aattttctgc ctgattaact gagatggcat ctttgatttg
     5701 ttcagcagtc attttcacat tgtcttctgt ttctaaacga tctttgactt cattgagcac
     5761 taaatcactg acgatcatat ctttatacgt attgatcatt tgcaggttcg tattgacatc
     5821 attgacattt gcagtgtctg attgtggcag tgtcacgatc aattgtgctt gtgaactgta
     5881 ttttggtgtc aagatgaaaa atgttacgat tcctgagatg gccaagccag caaacattga
     5941 gatgatgatt attaccaagt gtttgcgtaa tgtttcaaaa atttctttca agctgattgt
     6001 ctcttccatt ttttctccac tttcctattt attctctaga ttcatttgat cttttaattc
     6061 ttttttcaca cgatcaagtt cttgatcact gactttttga taggagatat tatcttgcat
     6121 gaagccttct ccttgcagct gatcttgctt aatcgtacca aatgcctccc gataatctaa
     6181 agcaattttt ttcatttcat caaagttcat gtctgttttc atattcgact ctagagcatt
     6241 caaaacggat tgatagttgc tgacaccgtt tagactcaat acttttttcg ctacgccttc
     6301 tacgatcttt ctttggcgcc cttgtctacc gtaatcacca tttgggtctt cataacgcat
     6361 acgagaataa gataatgcct gatccccgtt caatgtaatt ttaccgattg gaaagtcata
     6421 gccatcttgt gagaaagtga ggtcattatt gacttcgata ccaccaactg catcaacgag
     6481 ttcctttagc cctttcatat tgatagagac atagtgatcg attgggatgt ctacatattt
     6541 ttgtacggta tccatcgcca tggcagctcc gccgaatgcg taagcgtggt tgatcttgtc
     6601 ttttgtgtca cgacctacga tgtcgacata cgtatcacga ggaatgctga cgatcgttgt
     6661 ctgtttgtct tcaggactga ctgtggcaac catcatcgta tctgatcttc cttgatcgac
     6721 ccgacctaaa tctccagtat caatccccat caatagaaca gagaaagatt cttgtttgtc
     6781 cagatcaact ggtttcgttc gttctccttg ggttcgttcc actgattcgt aagttttctc
     6841 aatagaatgt gagagatcca gatataattt tgctcctact cctacaattg ctaatactaa
     6901 tacgataaga actgctaaaa taatcagaaa tatttttttc cctcttgatt ttcttcttcg
     6961 tcttgccata gtttcctccg tttgctaacc ttgcttgaat caacgtttta actgtcaata
     7021 gcttatattg ctatagccca tttgctcagc aacagtttgt gcttttgttg cgtatgctgc
     7081 gcttattttt tcattccgga tctgctctat ttgactgagc accgtcaaat attgatcgta
     7141 agtagcggtg gcagtcactt gaccatcctt taacatcgta tcgaataact gatctagttg
     7201 cgtgacttgc gcgatctgaa tatcagcgaa tcctagatat tgcataacaa ccgttttcca
     7261 aggactatct ttaaaaaagc tcatattttc acgtacacga gcgacatctg cacttgtcac
     7321 cttttctttg atgatgacat cgtctacagc ttgctgccag tttgatacat ttttagtaaa
     7381 taaatcgttg acagctgtct ggatcttcag ttttgattct gcactatcta actgtgtcac
     7441 gattgctttc ttctcatctt ggattgcaac agcttcttta ggcaattctg attctttgat
     7501 gtcgtaatca tctgctgaaa gtttgacaga ggatagctta tttgacaaac ttgtgattgt
     7561 ttcttctgtt atttcagggt ttagaaaaat tttttctttt gtactataca gcttgtctac
     7621 ttcttttgat aaagaagtca aggtgtcttt ttgattggtg atggcagatt ctgcataatt
     7681 gatccgttgc tgatgttgcc gctgattatt gatatggtat ccaaacaaac agatcgcaat
     7741 agcacctgca cctaccgcca cagcagtcaa tgacttgacg aatggctttt ccatagattc
     7801 actcctaagt attgatgata gataaattaa gatataattc acttacataa aaatgacagc
     7861 tttttattta aaaaaacata attaaactaa tatattcaca gaaatatttg taaacaagtt
     7921 ttccctatat aatcatacca caaactgttt tcttgactag ggcaatttac gatttaaaag
     7981 taaataaaac attaataaga taaaaattaa tcgatttcat ttttatttcc ataattttca
     8041 taggtgtttt taaaagttac acaaaaaata gaataacaaa aaaattaaca ttgaattaat
     8101 aatagtta
//
LOCUS       DAJSBN010000090         7958 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJSBN010000090 DAJSBN010000000
VERSION     DAJSBN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.091"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(7..2025)
                     /locus_tag="ONT86_002596"
     CDS             complement(7..2025)
                     /locus_tag="ONT86_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR2353342.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2038..3036)
                     /locus_tag="ONT86_002597"
     CDS             complement(2038..3036)
                     /locus_tag="ONT86_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353343.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            3234..4475
                     /gene="sstT"
                     /locus_tag="ONT86_002598"
     CDS             3234..4475
                     /gene="sstT"
                     /locus_tag="ONT86_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR2353344.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4622..5257
                     /locus_tag="ONT86_002599"
     CDS             4622..5257
                     /locus_tag="ONT86_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR2353345.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            5448..6188
                     /gene="truA"
                     /locus_tag="ONT86_002600"
     CDS             5448..6188
                     /gene="truA"
                     /locus_tag="ONT86_002600"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR2353346.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6333..7931)
                     /locus_tag="ONT86_002601"
     CDS             complement(6333..7931)
                     /locus_tag="ONT86_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353347.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTP
                     IYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTL
                     ILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2625 a         1239 c         1491 g         2603 t
ORIGIN      
        1 tatttgttag tcttttattt tttgtgtcgc ccatgttgca ctgtactggt tacctgcaac
       61 aagatcatat tgtatcagac gataatcttc tagaatttct ttttgtttct cagttaaatt
      121 tttttcttgg ataatttgac tatcctctgt aacaaagacc tgttttccat taaaacgatt
      181 agacaagctt tctgtaggat caattgttaa tgcaggtaga tgattagata cttctgtcaa
      241 caacgcatag taaggagtta ctttgatatt cgcatgctca agagccatag caccaaagtt
      301 attaggagca acaacttctt tatttaacgt agctgcttgt tcacgactat acttgttgtt
      361 ataaatgaaa taatctgtct ggtgctgttc taatccatat tttttcgaat cattcccact
      421 atagattgcg ggtaaatggt caccataaaa aacaaaggta atcggtttat cgattttgtc
      481 gatttcttgg atgaattttt ttactgctgt atcggtatag tgaaggcctt gcatataagt
      541 ttgtaattct tttcttctat taggaatgac agcagttcct tcagcagtgt agttcaattc
      601 actatagtaa tcatcataag gcatatggtt ttgcatagtg gataattgga tgaattgtga
      661 cgtttccgta ttgctttgta aagctttcaa ggtttctttg tatgctgaat catccgatat
      721 gcgtgggctt tgttctattt tatccgtata agttaattta tccggactgt ctaaataata
      781 gaacctgtca aaaccaaatt tatcaaacac ttgttttctg cgataaagag cagctgtaaa
      841 aggatggatg gctatttttt catcgaatag atcggtaata tttggactta ttttctgacg
      901 ctccactagc tgggtataag gtgtaactaa cgaagcagac aggttactga tactcaagcc
      961 tgtcagtcct tcccattcca tgttagctgt tccgccacca taaccgacgg ataacatcaa
     1021 tcctcctgtt ccttctgtta taatgctttt gatgtaagga atagggtctg taggtactgt
     1081 cagattaggg acacgtgcag gatcactgaa gctttcactt aaattgagaa cgatcgtctg
     1141 attgtttaac cagtcacttc ttgtttggtt gatttcatct gcctttgtgt tgtacttttt
     1201 cattattcca ttaatttttt ctttcgaata gccagcaggt tcttccatga ttttaatgtc
     1261 gatattgttt aggaattgaa tgactggtcc gttatctttt acggcgcctt gctggttaaa
     1321 gaaagtcttg tttactttaa ataaattaaa cagcaagaaa gaaggcgaat ttttgtgatt
     1381 aatgaaaaag acacccgaaa aacatcctaa taatacagcg atactgataa atcgacgttt
     1441 ccaatgaatc ttcaaggaat actgtttttg tagtctacgt tgtacaataa tactagtaat
     1501 caataatagg agaaggatca atccgcccac gattaataag actgggctaa ccatcgataa
     1561 tagttctgac aatccggtca gcatttttaa atctgaagga aagacaggct cttcccgcat
     1621 ttccattttg attactgtcg aaatagtgag cagtaagtta atgatcaagg tagcagtcaa
     1681 ggcgtaccaa aaacgattgg aaatcaacaa tagtaagaag aaaaatccca taataatgat
     1741 gattgtcagg atcatgggtg cttgtcgctg agtaaatatt tttaaggcaa cctggatagt
     1801 ttctttccaa ccttcttttg gttctaaaac gaaaatctgc acgaaggtaa agcagtaaaa
     1861 aaatgctgca actaaaaaat aacttttatg aacaaaaagc caatgaattc tttttttcca
     1921 acgctgagtt aattgttcaa tcgaatcaaa agcgaagtat tcgatgaaac gatgaatgaa
     1981 acggattttt tgtatataaa ataaaaagat aactaggagg gacactgcta ataaatatca
     2041 aataaaaaac tgcccgatat ttatcagcca tgccttccct gagtttggaa attcacgttt
     2101 cataaatgta gatacgcgat aagaaactaa agggaaatta catataactt gagtgacaat
     2161 caaaaataca gcaagaaaat ttcctcttcc ttttatacct aatttatcag atagcatttc
     2221 taaaaataaa aacgaagaga aagtataata cattgcgaag agagaatatg gtttagaaaa
     2281 cctcagcgct gtatctgtat ttcctctgat gatgagtgaa atttgtgtca tcaaaataat
     2341 caaaccgaat aaaacaatca agctgataaa tacttgagga agcttatagc ggaccttttc
     2401 tttccagttc cattctttca aaccataacc cattaaaaat aaataaaaaa tccataaaat
     2461 actctgtcca ttctgtaatg caaacgggtc tttattaaat agagtagagc agccaactaa
     2521 tccaattgtt aaaaataata gtagatattt aattgttgtt ttcgataatt tatttaaaaa
     2581 tccaactgta taaggaatac tacaaaaaag caaagtacaa gaaacagcat aagtaaagta
     2641 attctgactg ataggtgtta ggataagcca ataatcacga atattcaagc tgccagcttg
     2701 tacgtaaaaa aagatattgc aaactagtgt ggctattagg aatataaagg caaaacgact
     2761 taaataatgt aaaactttta aacgggcgat tttttgatat tggtaaccca tgtaaagtgg
     2821 gatcaaattt gcaccaataa caacggaagt ttgtaaaaat gtatagatgt agaatcgacc
     2881 agggtttgtt gcatatgtag gaatagtgac tgctgaaatc aatatacgct gtaaaaccat
     2941 cattatacaa gcgacaaaga ttgtcaggaa gccataactc tccttcttta tttttttcaa
     3001 gaataaacac ctcacaaaaa aattggacaa aaacataaga aaacgtttcc tgataagttg
     3061 cccattaata acatcctaac ttagaatatc tcaaaaaaca acgtgtagac agaaaaagta
     3121 caaaattagc aagaaatggt tgtttgtatt gttagaattt ttagaaaatt atacctttag
     3181 tttctttgca taaaatgata caatgattgt taaaattgtg aaagggtggt catttgttag
     3241 agaagttttt ggatatgtcg ttagttcaac gtattgttgt tggtatttta gtcggaatat
     3301 tgtttggtat ctttttcccc acatggacat ttatttcaat attgggaacg ttgtttgtca
     3361 gttgcttgaa ggcaatcgca cctattctgg tttttttctt gacgatggcg tcaattgcca
     3421 agcataaaat tgggaataag acattcgtga agcctatttt gatcttatat ttagtgggaa
     3481 cattgctttc tgcacttgta gcagttattg taagttcaat ttttactgtt cctattactt
     3541 tacaagaaac agtatctgaa aaagcaccgc aaagtttgga aagtgtttta tctactatgt
     3601 tgacgaatgt cacacaaaat cctgttcaat ctcttattga tgcgaattac cttggcgtct
     3661 tattctgggc tgtattgttg ggactagctt ttcgagcacg ttcagaatcg acaaaagagt
     3721 tggtggatca gatatctata gcgttgtcac aagtcgtaca agtgattatt gcatttgctc
     3781 cgatagggat tttaggattg gtttatcaat caattgctac gactggaatc gctggattgg
     3841 cagagtatct gcagctttta cttgttttga tcggtacgat gctgtttgtc gcattagttg
     3901 tgtacccatt cctcacgttt ttgtttatta aagaaaatcc ctatccattg attttctttt
     3961 gtttgaaaga aagcgcactt cccgctttct ttacccgaag ttctgccgca aatatcccga
     4021 taaacatgat gttggctgaa agattgaaat tgacaaaaga gtcttattcc atatcgatcc
     4081 cgttaggtgc tacgatcaat atgggcggtg ccgcgattac catttccttg atgacgttga
     4141 ctgctgtaca ttctttaggg attgaagcac cttttgtttt gaaagtcctt ttatgtattt
     4201 tgtctgcttt agctgcttgt ggagcttcag gtgttgcggg cggttcactg ttattgattc
     4261 cattagcatg tagtttgttt ggtatctcta atgatgtcgc gatgcaaatc gtcggtattg
     4321 gatttattat cggtgttatc caggattcaa tggaaactgc cttaaattcg tcaagtgatc
     4381 tgttgtttac tgcaatagga gagctaggcg cgcgtaaaag aagtggggaa aaaatcaaat
     4441 tgaaagattg tttagatgga ttaagtgaac gttgaacgtt agatatttag tatttttaaa
     4501 atgattcgag tttttaatga gttatataga tgttcgtgta aatagaggtt tctgttaaat
     4561 caagataaag tcgtttataa taacaattaa aagcataggt aattgttatt ggaggcgtat
     4621 catggatttt ttacgtaaat ttggtttttt ctttttatta ttaggcgtgc tgagtgattt
     4681 tttgactccg tacgtattag gattgtatta tcctgaatta gatcaaatga agagcgtgat
     4741 cagtgtattt ggagaggttt ccagtcctgt aagaaaagcg tttcttattt ggtctgtggt
     4801 ttcagggatc tttttcatcc tgtcattgcc tgcattgtat gctgcatttg ttagtacatc
     4861 tcgtgtgtta gctaccctag ttacagcagc catcgcgttc tatggtatag gtgattgtat
     4921 ctttacaggt ttgttcagta tcaataccca tgaatcttcg tggaatgtct cgacatgggt
     4981 ccataatatc ggatcaggtt taggttacag tggtttttta ttgtttccag ttcttctttt
     5041 cttgatctat cgaaaaaatg ggcaaatgga cgtaagccac ttataccttg gactgtctgt
     5101 gatcagtttg ctttttgcgg cgatttatgg actggcaaga attccttcta taaattcttg
     5161 gccattactg aataaaatag gtttttgcca aagaatcagt tttttcttta attatgtgcc
     5221 cattgccagg ttgagtatct tgcatctcaa acagtaaaac ttgagccgtc aaattgaaca
     5281 gtcagcggat acttcatccg gtggctgttt tttgtttttc tgaaatatat atttccacag
     5341 ttttcttgta tagcttctgt cttagagaag caaggagcta tattttgtgc tacaatctta
     5401 aaaaggtaga gagaagacca aatggaagac agaggagcca ctaaaatatg agaaacatca
     5461 agttgacaat cgaatatgac ggaaaaagat attcaggatg gcaaagactt ggagatgacg
     5521 ataaaaccat tcaaggaaaa atcgaaaaag tattgcatca gatgacaaat gaagagatag
     5581 aaattattgg atcaggaaga acagatgctg gcacccatgc gcgaggacaa gtagcaaatt
     5641 ttaaaacaaa cacagaaatt ggactggcag aaatgatcga ctttatgaat cgttaccttc
     5701 cgcgggatat tgtcatcaaa agaatcgaag aaatgcctga gcgcttccat tcaagatata
     5761 atgcggtggg caaaaaatac agctattatg tttggaacaa cgtgattcct tcagcatttg
     5821 agcgaaacca cagcttttat ttccctcaag agttggatat ggacaagctg aatgcagctt
     5881 gcgaaaaatt agtgggtaca catgatttca ttggtttttc tgcattgaaa aaatcaaaaa
     5941 aatccacagt gcgtacgatt gatgaattat ctatcgaacg aaagggagaa atgcttcatt
     6001 ttacttttgt cggagacgga ttcttatata aaatggtgcg gattatcatg gggacattac
     6061 tagaaatcgg cacaggtgcc atgccgatag aaacaatcga tgaaatattt gaaagcaaag
     6121 tgcggcaaca tgcaggagaa accgtccctg cacaaggtct gtttttggat gaagtttatt
     6181 atcgataaaa atactttgat aatggatgaa acgcatgttt cacagtataa atatcgatgt
     6241 taaataatgt atgaagcaaa ttaaagaaat ataaaaaaag actttttagc ctgtttatgt
     6301 gtacagctaa aaagtctttc ttaaataaaa atctagtgag aatgttgtac ttctcttctc
     6361 ggttgttttt gtttagaaaa agtaaatcga taaagtaaaa ataaaatcaa agtaacgata
     6421 gataaatatc ttgaagcaat tgctagtttt gtgtactgat aagtaataac gactttattt
     6481 atacctggag gaatagttaa ttcagttgtg ccaaacttag ataattttgt ttgtactagt
     6541 ttgccattaa tagaagccac ttgcccttta taacgataaa taggtgtcgt caagacagta
     6601 tttttattat tcgaattatc taattcaatt gtaagtttgt tactcataaa atgagttgtt
     6661 ggatttattt cctggttacc tagtaaatat tgatcattat taatcatatc aggatgatag
     6721 attgactctt tgtttgcata atcagtatga ttgtaatttt cagctatagc aacagcgtct
     6781 tcaggtgcta taagtgttaa tgaatctttg ttagcatgga tagaattgct tactcctgag
     6841 agatgcaata ttagtgcaag aaacgtaaag accattattt tccatgaatt atttttagta
     6901 gaaaaaatat aatagatcac tatactcatc gtataggcta taaataaaga agaaaaacca
     6961 tttaaccgcc agacaaattg tagactctta actggtgtat ggttaaaaag ttgccaagga
     7021 aataaattgg ttgaacaaaa aagaacaaat acaccaagaa agaaaataaa taaatcatca
     7081 gcagttaatt tttttataaa aataaaagca ccaatcaatc cgagaaataa gaataatcca
     7141 atagtatagt tgttatagtt attatttaaa atatgtgtca gcatatcact aggagccata
     7201 ccacttaatt cttttcccag tgggactttc aattcttgtg ctctagattg ttgaataaaa
     7261 ggaattaaga atcccgcact taaaaaaaag gtgaggacgg tagcttttaa aagacttaag
     7321 agtctagcga ttttttggtc ccaaaaataa aaagaaagaa acaacgttcc tccaataaat
     7381 acagaaacca tagcgacaga aagtaaatga gtatacacta ctagcgtcat gccaatagac
     7441 aaccagtacc atttttggta atctctaata taaatttcgt aacaccccat tagtatcaaa
     7501 ggcaaaaatg ttaaagcgac agcttctccc aatgaagcac gatggaatat gtcaatggca
     7561 cgataggcgg aaaatgtata aataatcgaa aataacagcg aaatataacg attgtttttt
     7621 atttgtttca tagaaaaata agaaacgacc attgttaaaa aagtgataaa aaaatagtag
     7681 atattataac ttaaaacaag atttcccatc attttataaa ataaaaaagc tggataaaga
     7741 gtaagccatg gataaaaaat attcatcatt gttccatgat gatcgaagtt attgaagttt
     7801 actggagaac tccatacatt atctagtcct atgattcgag ataaatgaaa gtaagtatcc
     7861 tgattgacac tatgatagat tcccttagac atgaatagag ggatgacgta agcaaaactt
     7921 agaaataaca taaaaatgat atctattatt tggttttt
//
LOCUS       DAJSBN010000091         7633 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJSBN010000091 DAJSBN010000000
VERSION     DAJSBN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.092"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(63..791)
                     /locus_tag="ONT86_002602"
     CDS             complement(63..791)
                     /locus_tag="ONT86_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2353348.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(804..2084)
                     /locus_tag="ONT86_002603"
     CDS             complement(804..2084)
                     /locus_tag="ONT86_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2353349.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2225..2827)
                     /locus_tag="ONT86_002604"
     CDS             complement(2225..2827)
                     /locus_tag="ONT86_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR2353350.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3072..3278
                     /locus_tag="ONT86_002605"
     CDS             3072..3278
                     /locus_tag="ONT86_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353351.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3590..4309
                     /locus_tag="ONT86_002606"
     CDS             3590..4309
                     /locus_tag="ONT86_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR2353352.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4390..5073)
                     /locus_tag="ONT86_002607"
     CDS             complement(4390..5073)
                     /locus_tag="ONT86_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCR2353353.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5291..7477
                     /locus_tag="ONT86_002608"
     CDS             5291..7477
                     /locus_tag="ONT86_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR2353354.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2736 a         1284 c         1438 g         2175 t
ORIGIN      
        1 aaaaagaacc gaagggtttg gatcggctgg tcatttagtc ggtccaaact attcggttct
       61 ttttatgaaa tcaatttctt taatccacgg atagaggctg ggatggtctc gccgtttgcc
      121 aacgtgactt ctctggtttt tttattaatc gaccgcacat tttgtgtatt gataacatag
      181 gatttatgac aacggataaa attccgatgc attttctcga tttctttgat cttctcatag
      241 aattgtaatt gggcatgagt caaatggact tccagtttat gtggcgctcc tagcgaagta
      301 gaaaaatatt ggatgtcttt gatcggtaaa aatttgatct catcattaaa ctctattttg
      361 aagatatctt tttggatttg ggacatttga aaatcttcga aaattttgct caggatatgc
      421 tcgacatttt cttttaaatc ataaagatct tctttgacga tataatcagt cggttcgatg
      481 ttgcttttta agattttcat cgacatttcc gtatgggaag tcacatagat tatttttcct
      541 aaaggatcta attcacggat caatttaccg atcgctacac catccatttc actgtcttct
      601 agttcgatat ctaaaaagta aataccaaaa gaagaagttc tgatcttggc tgcattgaga
      661 agttcttccg gatcagaggt agaaatatgg acataactgt ctaagttttc aatcatgatt
      721 ctatttttga tcaccgttga taaaacttct aattgttttg gatcgtcttc acaaatataa
      781 atgggaatca tatcattact cctttatttt atttgtatat aaatcgtttg agaaaaacgc
      841 tggttttcaa ttgatgtttc aaggaaaaga taaggattgg tctttgtcaa ttcatccaag
      901 ataaacaagc caaggccttc gttttttgga tttgtttttg ttgagaaccc tttttgtttc
      961 aactggttta acggtaatgt atttgttgtt gtattgatca cacgaatcat cacggaatga
     1021 ttcttcttga gtatggtcaa ttcgattttt ttcttctttg cagcactagc tccttcaatc
     1081 gcattgtcca agataatacc aagcatacgt acaaggttta ccgtatgttt cgtatccagt
     1141 tcgattggct caggaaaatg aagctggacg atcaaccctt tttcctgtgc catcatgagt
     1201 tttaatgaca gtaaactacg gatctcagga actttcatat tatccaacat acttagattg
     1261 gccggaaaaa ggttcatcat ctggcgtgta ggtaatatcg tttgatcata ataagaccgt
     1321 aatccttcta agtcgccttc tgaaatatag ctgtgaagag aaaaaagcag attttggtaa
     1381 tcatgtttga attctctcaa gtcttgatag tttttttcta gatctttgat ataatcctgt
     1441 tgcatcctga cttgttgctc taataactga tcagctaatt tcttattaga gattttccaa
     1501 taataaacca agatgatcag gccaacacac agatatagaa tgaaaaaact cgtattcaaa
     1561 gctaagacat caggtgtttc accagaaaaa cgggtataaa aaataatgat atagtatgta
     1621 aataatatca atgtcccgat cgtaccgatc atacggcggt ccacagtgtg aaaccaagtc
     1681 tctaacgatt tttttaaaca aaaagctaat aaagcagata aaaaagcgga taagacaatg
     1741 tgaaaatact gcaatgaatc tcctggttca ataaccgaag atttcaaaac aaaaaaatcg
     1801 tcgatgcttg ttaagtgatc tcctaaaagc gacgtcaata acccaaaagg aagcgcgtaa
     1861 accaattgtt gacgcgaagt cgaaaagaag aaaaaagtaa gcatcagcat tcctatagca
     1921 attagagcag taaaaatccc taaaatcgaa tagacccatc caatcaaaat acaagaaatc
     1981 agaatgactc ccgcaaacag atatttgtat cggatgtgat ggtcaatcaa taagatacaa
     2041 attgtgtaaa tcaaatattg caacgctatg gaccataaaa acataaagta cagctcccat
     2101 tgatttttac caaattatag cacaattagc gtattatttt tttaattttt cagggatttt
     2161 gaattcagaa ctcataccaa agcagcagcc agatacaaaa aagtcagata agttagttaa
     2221 ttgtttaatg attgtttttt tcatgaaaga ttcctccaaa attcatcaag tagtttgtaa
     2281 taggaagcaa cgtagctatt tctaaaacaa tcccaagtag caaataggta gaaaagtaag
     2341 aagctgagaa aaataaagta atgagagaaa aaattagaaa catgctgatc gccttcttta
     2401 ataatggggg acgtttctcg ggttcaatgg gattgattgc tgtacctctt gatccatagc
     2461 gaagcagtaa caaaaagcag ctgattaaca aaaagaccat cattgggaag gagatagtca
     2521 attgcagatg tttcatcacc aagggaacgc cccataaata aagtagtgtc cacagcagac
     2581 aaacgaattc agagtcggaa tgcaatccat aagcatatct gcggatgcag atataagaaa
     2641 gaaaggtgat tcctgtttcc gcccatgagc ctgtcaccag agaaaagact agaacgaccg
     2701 ctagttttcc aagattattc aataaaactt ctaaggcaaa ttttacttgg acatacacca
     2761 tatcttcttc cgatttgtct ccgctgaata aggcttctaa taatttgttc acaactttat
     2821 ctttcatctc atacgtacct ccaccacaaa ttttatgcta aaaaaaataa aaaacgtctt
     2881 ttgtttcata aaaagtggtt ttgacatcaa aaccgtcatg atctcctaaa aaaatcttcg
     2941 ttttccaaga accttaccaa aaaaagtgac agttttgata cgaaaagtac tttttatgac
     3001 aagtttgtgc tttttcgagt gatttatgtt aaaaaattac tgaccttata aaaggtgatg
     3061 gagggattct tatggaaaat gaacaattgt ttgaagtcgg cgaaacatat gcatttaaat
     3121 ctgatttatt tgaatcacca ataaaaggaa aaatagaaaa aatctatgaa aatagtgtga
     3181 tgatacgtgt catgaaatgc gcagtagaag acaatgatac cgctcataat ctgcactggc
     3241 agttagttgt acgcaaatca agtacaatag aagaataaaa acagataaaa tgttgagagc
     3301 ataaagaggt tgtgagtctc ccgtttagct ctgagttaca aggaataatg ttaaaaaaca
     3361 gcttttcatg ttttatgaat attgccttgt gaccgaggtg ttggcaggag aatactgttt
     3421 atttgaaaaa agggctgcga caagactttt gtcacagcct ttattttcat gaataggagt
     3481 tacttttggc tacttttaga gaagtagctt ttaaggagca gataaagtca aggcttttat
     3541 ttctataaat gaagtagtaa aatgaagaac agattttatt acaggaagga tgccgataat
     3601 tgtgatcatt gttgcaggaa cgatcggtgc agggaaaagc acactaactg aaatgcttgc
     3661 ccaagattta gaaacaaaac ctttttatga gaacgtggaa gataatgaag tattgccgct
     3721 attttattct aatccagaaa agtatacttt tttattacag attttctttt tgaacaaacg
     3781 ctttttggca atcaaagatg ctttcagcca tgatgacaac gtactagacc gctccatcta
     3841 cgaagatagt atgctgtttc atttgaatgc agatttaggg cgtgtttctg aagtagaagt
     3901 aaagcagtat gaaggtcttt tagaaacgat gctgaaggag ttagaagaaa tttcgccaca
     3961 aaagaaacca gatctactgg tttatattcg tgtatccttt gaaactatgt tagcgagaat
     4021 caaaaaaaga ggccgggaat atgaacagct agaacaagat cctgaactct attcctacta
     4081 taaagaactc aatcgacgat acgaagagtg gtatgaacag tttgatatat gtcccaaaat
     4141 agtgatcgat ggggacaaat atgatttcgt agccgatccg gcatgtggag aacaaatcgt
     4201 acaagaaatc aagatgcggg caaagaaaat gacagaagag gcagatgccc agtcgatccg
     4261 ctacactaca aataacccga aagcaaaggg aatctttaca aataaataat ataaaaacta
     4321 agcaaattgc tcactcgcag atatttctct gtgagcagca tcttgcttag tttttttgat
     4381 tcattgagat caaatagtga accgattcgt atgataaaat tgccataaat gaaatgtctg
     4441 agtgagggcc atgatcaaaa ataaaatgga aacgatcaaa atcgaatggg cgccaaacag
     4501 atgatgacag aaaacaagga caatagctcc tgcgataaag gcaagaacga cacttagata
     4561 gtggaaaaat cgatcaaaat ctgctttttc tttagaaata caataatcat ataagttcgt
     4621 tgctacccct tttaaatttc cagttgtaaa taaattagtg taggcacgcc cgttgatttt
     4681 atcaaaagct acccactgga tcgatgcagt aaaagcaatc aaaatcgtta ctaatgcggg
     4741 atgctcatgg acgggtgtca aactaacaaa gaaaaaaatc actgcttcat aatatagaac
     4801 aaacaagcgc cagaaatgtt tttctttttt tctaaaataa tcaattagat actttgttag
     4861 aagaatccct aagaaaaaga aaagggtaga caatactttt ttaccaacac ttagccattc
     4921 tccgtcaaat gcttggatga tcgatagaat gatattgcca gtttgagcag aggcaaaagc
     4981 accatattgg atataagtat aactgtccat ggctcctccg ataaatgtca gcaaaagtcc
     5041 tattgtacgc ccctcgtgaa aggaagtctg catttttttc atcacctttt atgaatattt
     5101 ttgtcttcct tatcttagca taaacgattg aaaaaaagga aaaattttca gaaaactaaa
     5161 atcatatcgc ttcccatttt catttttaga aaggtctgtt agtataaaga aaaaatagac
     5221 aacgaaactt taaataacca acttgaaata aacaaattca aagaagaaag atgggaagga
     5281 aatcaatcag ttgaatgaaa aagaaattct ccgattacaa aaagaattga aacgctggga
     5341 gaacaagtat aaaaagcttc aacaagtctt tcggctacaa gaagaaatca ttgctaccta
     5401 tgaagcggct tatggcgaag cacccggact tattgaggaa gaagtacaag taaaagaaag
     5461 tgaaggaaac agctctttct ttaaaatgat ggagaaacgt tcctataatc gcttcaatca
     5521 agaacaaaaa gcagctatca catatgatat gaagaaacat cttcgaatca tagcaggagc
     5581 tgggagcggc aaaacgcaaa caatctgtgc aaaagctgtt tatctgatga cgcaaaaaca
     5641 agtagacgaa gaacgcatac taatgatcac ttttacaagg aacgctgcaa atgaactaaa
     5701 aaaacgagta gataatttca gccagagaaa aaccgctgtc catatcggaa cattccatag
     5761 tatctttttt aggatataca atgaaatttg tcgaaaattt cctgaagtag caatgcaagg
     5821 aatacaagga gacttttcaa aggatactgc acaaaaagtg aatgctgttt tgcaacagct
     5881 gatacgtaaa tataatcttt atatatttga tcaatatgga gaaaaaacga ttgcttctcg
     5941 gttagactat tggcagaata tgaacctttc agtagaagaa atggttcagt tagttaaaga
     6001 aaaatatgat tccattgata agaatgcacg acagccgata tcagaacgac tgtatggatt
     6061 gctcacggaa ctccaagaac aaaaaagaag ccagcaattg ctagagttca atgatgtact
     6121 ccaaaatctg aaaagcgctt tggagaatga ggagatccag cattatatcg gccaaaaata
     6181 tgattatcta ttcattgatg aatttcaaga tactaatcca cttcaatggg aaatcgtgaa
     6241 acggatgaca aaggaaaaca ggatcaagtt gattgttgtt ggagatgatg atcagagtat
     6301 ctatggattc agaggatcag agccagcata catcaagaac tttgaaaaag aatatcctac
     6361 caaaactctt ttcttattga ctaattatcg ttctcgggca gctattgtcc aaggagcaaa
     6421 tagattgatc agctataata aaaatgatcg gataccaaag gcgatgattc cagcacaaaa
     6481 agaagcaggt gttatggaag cctatttgtt ttctgatact aaagcagaaa gtcagtggct
     6541 aatcaaacaa attcagacat ttattcaaaa aaatgggaaa tataaggaat caatcatatt
     6601 ataccgatca gcttcacaaa cgcagcagct tgtccaatct ctgctgaaaa cgaatattcc
     6661 ttttgtactg gaagcagata gtccatatga aggtatcttt ggtatcaagt ctttccaaca
     6721 tttctatcaa aaaattgttc attggcaaac agcactgaat ctgcaaaaga aaaatcaggc
     6781 ctaccagcaa ttgctccggc agctgatggc agactgctac ctgaaaaaaa gtgaatgcga
     6841 tcaatacttt tctcctaaat atcaaaatat agccgttgca gattatattt tgtcgataag
     6901 ccccaattta aaatcgaaat ctgcagaaat ccgagagttt gaggaagcgt tgcgtaaaac
     6961 agaccagcat caagaaatct attcgcttgt tcaaagctat cttcatctgc cacgtatagc
     7021 taaggaaatc gaccagagtg atcaagaatg gatacgagaa gaagtaaagc agcatgctac
     7081 tttcagaaga ctccaagcct tatcgcaaga aacccaagcg acagctaaag aattaaaaac
     7141 ccgcttgatt gcttacagac aaggaaaact tgatgcacta tgtatccaga gcattcacaa
     7201 aagtaaaggt cttagttacc gaaatgtctt tttgattggt tgtaacgaag gaagtttgcc
     7261 ttataacgga gcgacagaaa agaaagtaat cgactttaat gaaataaaag cagaaccagt
     7321 gacgacgata gaagaagaac gaagactatt ttatgttgcg atgactagag caagaaatcg
     7381 tctgtatcta tgtgtacctc aaatgaaaaa ttcaagaaaa ttgaaagtat cgagatttat
     7441 caaagaaacc gggagtcgtg tgaaaaaagg aaagtgatag aagagagggt gtgacagaag
     7501 tggtcagttt caagaaagaa gaaggaatgt acgaaaattg cttctcaaat ttttgtgaaa
     7561 tttcagctta ttttcgaaga agctatttct ggaacacagt ttattcggaa aagggaggtg
     7621 tgacaagaat ttt
//
LOCUS       DAJSBN010000092         7600 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJSBN010000092 DAJSBN010000000
VERSION     DAJSBN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.093"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(943..1356)
                     /locus_tag="ONT86_002609"
     CDS             complement(943..1356)
                     /locus_tag="ONT86_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCR2353355.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ONT86_002610"
     CDS             complement(1812..2297)
                     /locus_tag="ONT86_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353356.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ONT86_002611"
     CDS             complement(2436..2651)
                     /locus_tag="ONT86_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HCR2353357.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ONT86_002612"
     CDS             2875..3675
                     /locus_tag="ONT86_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2353358.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ONT86_002613"
     CDS             3694..5562
                     /locus_tag="ONT86_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR2353359.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ONT86_002614"
     CDS             5555..6046
                     /locus_tag="ONT86_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR2353360.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="ONT86_002615"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="ONT86_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR2353361.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="ONT86_002616"
     CDS             6606..>7600
                     /locus_tag="ONT86_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR2353362.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAJSBN010000093         7483 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJSBN010000093 DAJSBN010000000
VERSION     DAJSBN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.094"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            4..1077
                     /locus_tag="ONT86_002617"
     CDS             4..1077
                     /locus_tag="ONT86_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353363.1"
                     /translation="MLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEIAKKIE
                     EKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFDEFICF
                     SMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLKKRTPL
                     YNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTS
                     VYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEA
                     IQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERL
                     LKQKTMKNILKNVKIAESLDDIL"
     gene            1074..2495
                     /locus_tag="ONT86_002618"
     CDS             1074..2495
                     /locus_tag="ONT86_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR2353364.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            2531..3247
                     /locus_tag="ONT86_002619"
     CDS             2531..3247
                     /locus_tag="ONT86_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR2353365.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            3266..4849
                     /locus_tag="ONT86_002620"
     CDS             3266..4849
                     /locus_tag="ONT86_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HCR2353366.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            4984..5562
                     /locus_tag="ONT86_002621"
     CDS             4984..5562
                     /locus_tag="ONT86_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353367.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            5508..6026
                     /locus_tag="ONT86_002622"
     CDS             5508..6026
                     /locus_tag="ONT86_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353368.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            6034..7074
                     /locus_tag="ONT86_002623"
     CDS             6034..7074
                     /locus_tag="ONT86_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR2353369.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            7323..>7483
                     /locus_tag="ONT86_002624"
     CDS             7323..>7483
                     /locus_tag="ONT86_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2353370.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         2747 a          854 c         1410 g         2472 t
ORIGIN      
        1 ttagtgttag caaatacata ttatcaaata atagtagcaa tacaaatgaa aaatacaatt
       61 ttttccgatg agtatgttga aattattatt tctgatcatt ctaaaaacac taaagaaatt
      121 gcgaaaaaaa tagaagaaaa aaaagtgttt gatggctgtc attatttaga aagcaaatct
      181 tttgatgata atattgggta ttttcaaaag attaaggact gtcttcagat ttcttgtggt
      241 aaatcaaatc gatatgccta ttatttgaaa aactgtaaaa gtgaatattt tgatgaattt
      301 atttgttttt caatgggaga ggtttggttg gaaggaatat atgcttgctt atatcgaaat
      361 aataaaaatg tacaggtatc aattttagaa gaaggtattt taagctatgg agttgacatt
      421 attccaacag caagaacaaa agctatcgca gtactcaatt acttaaaaaa aaggactcca
      481 ctatataaca aaagctataa tttttattgt ttttttccta atatatatac aggaagagag
      541 aatacaatac aaattccatt agttacagct gggtcaagaa ccgcattgca actttcggat
      601 atttttaacc ctatgataga tatttataaa gaaaaaaaat atatattttt tacgtctgtt
      661 tatgattttg agggcggaga acctgtcgga gaatatgaat tagtatgcaa aatagcagac
      721 ttagtaggaa aagataatct aattgttaag acgcatccta gagatgtaag aacaatttat
      781 aaagataatg gatttatcga ggataaaaat tcatcaatac cttgggaggc gattcaattg
      841 tcaggcgatt ttaacgataa aatatttatg acaattaatt caggaagtgt tatttcaggt
      901 agtacaatgt ctgcaaaacc tgtacgcacg tattatatgt ataaattgtg taacatagaa
      961 ggaaatccgt cttgcaaaaa gaatgcgtat gatattgagc gattgcttaa gcaaaaaacg
     1021 atgaaaaaca ttttaaagaa tgtgaaaata gcagaaagct tagatgatat actatgagca
     1081 aaactaaata tttaattaaa aatatgggaa tattaacaat aagtaatttt tcttcaaaaa
     1141 ttttagtttt tttgttagtc cctttatata caagtgtatt atccacaaca gaatatggca
     1201 catatgattt gacaatttca acagccacat tattgtttcc aattttaacc ttaaacattg
     1261 tagatgctgt gatgcgcttt ttaatggata aagataaaaa taaaaaaaat atagcatcta
     1321 tagcgattaa agttatattt ttaagcgtga tagtctttgg atccgtaatg tatttgttaa
     1381 atattacaaa attatggcaa gatgtttatg gattagaaaa atatattttt ttgtattata
     1441 tatcctatgt atggaatcaa tttctaatac aatttgctaa agggttagaa aaagttaaag
     1501 ctatgggcat agcaggtatt atcagcacaa ttgtaacaat aattgccaat attttcttct
     1561 tgcttattct gaaatgggga ttaattggtt ttttcattgc aaatatttta tcacagatta
     1621 tttcagctac ttatctgtca gtattagtga gaatatggga atatatcgat tttgaggcta
     1681 aagatcctca gctaaagaga gaaatgctaa attactcgat tccattaatt gcaactgttg
     1741 ttggatggtg gattaatagt acagctgata ggtatgtggt ttcctttata gtaggtgtct
     1801 cggcgaatgg cttattgtct gtggcatata aaatacctca aataattaat actttgcagg
     1861 gaatatttat acaggcctgg caaatttctg caattaaaga atatggagat aatgagactg
     1921 caaaatttta tggaaataca tttgttatga ttaatttgtt aatgtgcata acctgttcaa
     1981 gtcttatcat attaactaga ccgttttcac atattcttta tgcgaaagac ttttatgaag
     2041 cgtggaaata tgttccattt ttattaatag ctaatgtttt aaattgtgca tcaggtctgt
     2101 taggacctat cttatcagca aaaaaagatt ctaaatccat gatgtggtct gcaatcatag
     2161 gggcattagt taatgtgatt atgagtgtag tccttgttta taagataggg gttcaaggtg
     2221 ccacaatagc cactgttata tgttcatatg taatatatgt tgttcgtaaa cgagctgtgg
     2281 gaaaagatat ttttataaag cattatcata ttgttgtgga atcttgggga ttattgtttg
     2341 ttcaggcaat catagaaata tattttaaaa attatgtaat agaaattata attatgatta
     2401 ttttaattat tataaatatc agttatatta agcagttgtg ttatagagga agaaacatta
     2461 tttcaatgat taagagtcga gagcacaaac ggtagacgat gttattttta taaaaagata
     2521 taatttatta atggtcgata attatgaaag gaaaaatatt cttaaaaaaa agagtatagg
     2581 atgtcgtatg aaaatagtcg ctatagtacc aatgaagtta aataacaaaa gattgccaca
     2641 aaaaaacacg aaagctttta ccaatggaaa accattgtgt tattatatac tttcaacact
     2701 tttaaaagta aaacagattg ataaagtgta tgtctattgt agtaacccag atattaaaga
     2761 atttatacca gaaggtgtaa catttttaaa gagatctagt tccttagatc aggatacaac
     2821 gagtattaca caggtattag ccggtttcac aaaagaagta gaggctgatg tatatgtaat
     2881 gacacacaca acagcaccat ttattagcga aaaatctatt gaaaaaggac tagatgccgt
     2941 gctgacagga gaatatgatt ctgcatttgc tgcaaaaaaa ttgcaagatt tcttatggaa
     3001 agacggtgaa ccatttaatt atgaattgga caatattcct agaacacagg atttgccgcc
     3061 tttatacgaa gaaactagtg gattttatat ttataatcgc aatgtgatga ccgagttgca
     3121 tagaagaatt gggaaaaaac cttgcatagt tgaagtaggt gagattgagt cagttgatat
     3181 tgatgaagcc gaggatttta tgattgctga tgcgatttat aatcatctta ttttacaagg
     3241 tagttaagac agggggaagg ttaggatgaa caatgtacag attttagatt gcacacttag
     3301 agatgggggc tattgtaaca attgggaatt tggatttgat aatacaaaaa aaattatttc
     3361 tggacttata gaatcaggaa ttgagattat agaatgtggg tttctcacaa ataaaacagt
     3421 atacaataaa aatgttacaa aatatacgac cttagaagaa ttagcacaga taattcctga
     3481 aaaaagagaa ggaaaattat ttgtagcaat gatgaactat ggcgaatata atatagagga
     3541 tcttccagat tatgatggtt catctattga tggaattagg attgcgtttc ataaaaaaaa
     3601 ttgcaatgaa gcgttgaaat tatgtcgtga gataaagaaa aaaggctact tagtttttgt
     3661 tcaagctatg gtatctctca attatacaga tgaagagttt ttagcagtaa taaaaaatgt
     3721 gaatcagttt aaaccatacg ctttttatat tgttgatagt tttggaatga tgaaaggaaa
     3781 agatttaact cgtctttttt acatgattga gcataatcta gaaaaagata tacagatagg
     3841 ttttcactca cataataaca tgcagcttgc atattctaat gcacaaaaac ttataaccgt
     3901 acaaacaaat cgaagcctga ttattgattc ttcggtgtac ggaatgggaa gaggagcagg
     3961 taatcttaac acagaactat ttgtagaata tttgaatgat aatgcaggaa aaaaatatca
     4021 attaaaacca ttattatata taattgatga aatactaaat agtttctatc agaagagtta
     4081 ttggggatat tcattaccaa actatatttc agcagttcat aatgctcatc ctaattatgc
     4141 aagctatctg tcatataaga atactttaac aattgaagca atgaatgaaa tattcgaaat
     4201 gatggatgaa gaaaaaaagg tatcttatga taaacaatat atagaagatt tatactacaa
     4261 atatatggaa actgggaaaa tacaggagga atatatggaa gaactgagaa aatttctctc
     4321 agacaaaaaa attctactga ttgcaccagg aaaaagttct gtagatgagg tagaaagaat
     4381 aaaagagttt gttaccaaag aagaagtcgt aataattagt gtaaattttg aatattctca
     4441 tatagatata gattacgtat ttttaagtaa tttaagacgt tttcatgaat taccgaaaga
     4501 aagcagaact aaatgtattg ttacatcaaa tatacaagca tcggatgtat atttacggac
     4561 gagttataaa aagttgttat ctgaaaggga agttgtaaga gacaatgcag gattaatggc
     4621 aattagattt ttagccgata tgaatgtgga tgaaatattc ttagctggat ttgatggata
     4681 ttcacataat gaagatgaga attatggtga gcaatccatg gaaattataa caaaatttgc
     4741 attattagat gcaatgaacg tgcaaatgag agatgaattg tcagaattag ctaaaaaagt
     4801 aaaggtgaca tttttaacgg atccacgttg ggttcgaatt gataagtagt agtaataatg
     4861 tttttaaaat tttgataaag agatagtgag agaataaaaa tgggtaaagt cttaaataaa
     4921 aaagaagaaa aatatttaag ggacgatggg gcgagatgaa actctaattt tgaactttta
     4981 agaatcattt ctatattgtg tattgttgct taccatgcta atttgcgttt aaattataat
     5041 gcagacggaa ttatagtaca tggttcatta actgctaacc agattttctt gaattatatt
     5101 ggtatttggg gtcagctagg agttaattgt tttatattta tttcggcata ttttttaagt
     5161 gagaaaagaa acattcactt tgaaaatgtt ttgggaattg tattagaatg tattttttat
     5221 agtgtattag tttatggaat tctttgtgca tgtggaatag aaacattttc attgaacagc
     5281 tttgtaaaag aaatattatc atttgctcta ggtagatatt ggtttattac atcctattta
     5341 gcattgtata ttctaagtcc gtatttaaat atcatgctta acaatctgga aaaacttcag
     5401 ttgcaaaaaa taagtatgtt attgtttata ggtgtatgct gttatcgtat gttatttaaa
     5461 acatctttgt ttggggattg tgctgtttta attgcactat atgtcatatg ttatacactg
     5521 aaaaaaacga taataatatt tttgagagac acagaatcat aggaacaatt gtatgtataa
     5581 cagttatacc acttctcata tccataggaa tacttttcaa atttatacca aatgcaatgg
     5641 ttaaccagat ggttcatatt ggttctccat tgatggttat agcaagtata tttttatttt
     5701 atgtatttaa aaatttttca ttttacagta agtatataaa taaacttgcg agaaccagtt
     5761 taggggtata tattttacat gaaaatcatt atttcattga tggtatgaag tctagaggct
     5821 taatatggga tgggatattt ggattaggca aaaggttttt agatagggat ttttggattt
     5881 ggtttataat tgcagttgta gttgtgtgca taatttgcat ggctatcgat gttgtaagag
     5941 aaaaatttga aagacagatt tttaaaatta agagtgtaaa gaacctgtgt aaacttatga
     6001 atcatgttat aaatatatac ccataattaa taaatgagga gaaaagaagt gaaagttata
     6061 tcattcggag aattattatt gagacttgca gcacccggtt attcaaagtt atttcaaaga
     6121 gatagtttgg agactacatt ctgcggaggg gaagctaatg tagcggtatc cttagcaata
     6181 tttggatatc aatcagaatt tataacaaag cttccggatt cagatgttgg atatgcggca
     6241 gctaatgctt tgcgttattt tggagtaaat gttgataata ttatatttgg agagggacgc
     6301 atgggactgt attatttaga aaaaggagca gctcagagac catctaaagt aatttatgac
     6361 agaaaatttt ctgcaatttc gttagctgat agtgatgaat ttaagtggaa ggagatccta
     6421 aacgatgcaa attggtttca ttggacaggg ataaatccag cattgtctga gaatctcttt
     6481 gagacgtcct tagatgcatg tcaggctgcc aaaaaaagag gtataacaat ttcttgtgat
     6541 ttaaattatc gatccaagct ttggacatca gaggaagcaa aagaaaagat ggaacagata
     6601 ctaccattgg ttgatatttg tatttgtaat gaagaagatg cagaaaaagt gctaggaatt
     6661 aaagctgagc atacaaatat taatagtgga gaattaaata aagatggcta tgtgcaggtg
     6721 gctagggaga ttcacaagaa gtttggatgt aggtatgtag ctacaactct gcgtagaagt
     6781 tattcggcaa gtagaaatgg ttggagtgca atgttatacg aagcagaaaa agataaagcg
     6841 tatttttcca cagaatacaa catacaaatt gttgatcgcg ttggtggtgg agacagcttt
     6901 tctgctggta taatatatgc gttatgtgaa aatatgtccg gacaaaaggc tattgaattt
     6961 gcgactgcat ctagttgttt aaaacatact atagaaggtg attttaatcg aactacaatt
     7021 gaggatgtat ataatttaat gaatagcggt ggaaatggtc gcgtgttgcg ttagtcaaca
     7081 tataaagata aatcacagta atttttatgg tgattatctg tcttagtagt gattagaata
     7141 acgtgaaaat aaattgttga gcgtttatac tgttttgaaa aataaattta acatattgaa
     7201 aaatacaaat atatacgtat attataaagc gccgattgta aaattaagct agacaactaa
     7261 aacagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag gagtgagtgc
     7321 aaataaccta tacctatctt acaacggatg aacttgtaat aatagagtct tatttgaaaa
     7381 ttaaccaatc tgttgctaaa actgcgcatt gcttgaatcg ttcaagtcaa acgatccata
     7441 aagtatatct gttcttcaag caaagaaaat cagccttaga ata
//
LOCUS       DAJSBN010000094         7431 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJSBN010000094 DAJSBN010000000
VERSION     DAJSBN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.095"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(113..790)
                     /gene="nth"
                     /locus_tag="ONT86_002625"
     CDS             complement(113..790)
                     /gene="nth"
                     /locus_tag="ONT86_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR2353371.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(797..1495)
                     /locus_tag="ONT86_002626"
     CDS             complement(797..1495)
                     /locus_tag="ONT86_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR2353372.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1763..2947
                     /locus_tag="ONT86_002627"
     CDS             1763..2947
                     /locus_tag="ONT86_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HCR2353373.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2953..3396)
                     /locus_tag="ONT86_002628"
     CDS             complement(2953..3396)
                     /locus_tag="ONT86_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2353374.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3622..3909)
                     /locus_tag="ONT86_002629"
     CDS             complement(3622..3909)
                     /locus_tag="ONT86_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353375.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4080..5432)
                     /locus_tag="ONT86_002630"
     CDS             complement(4080..5432)
                     /locus_tag="ONT86_002630"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCR2353376.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5705..6151)
                     /locus_tag="ONT86_002631"
     CDS             complement(5705..6151)
                     /locus_tag="ONT86_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR2353377.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6387..7313)
                     /gene="cysK"
                     /locus_tag="ONT86_002632"
     CDS             complement(6387..7313)
                     /gene="cysK"
                     /locus_tag="ONT86_002632"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR2353378.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         1999 a         1528 c         1226 g         2678 t
ORIGIN      
        1 tagctactga gtatgtacat ttactttttc atgcaaaaaa gataaacgaa acagcttttc
       61 tccataataa caagataatt catacattta tttgcaatta aagaaaccac gtctacaggc
      121 gtggtttctt ttttagcgcc ttttctttta gacgcatccg atttttccca tcttgacata
      181 ttgatagcaa aggacatacc tcgcacttgg gatttcttgc cgtgcaatga tatctaccaa
      241 aaaaaatcag tgtatgatgt gtctttaccc ataattcttg tggtactttc cgcatcagtg
      301 tttcttctac ttccatgacc gtagcatcta gtttgcagat cctcaggcgt tttgagacac
      361 gttccacgtg ggtatctact gcgattgctg gtatcccgaa tgcatccccc aatacgacat
      421 ttgctgtctt tctgcctaca ccaggaagag acatcaattc ttctcttgac gttggcactt
      481 gcccatcgaa acgttcaatc agctgctgtg cacacgcttt gatgtttttt gctttatttc
      541 gatacaaacc aatcgttttt attttaagaa tgatttcatc gattgaagct tctgctaacg
      601 catcgggtgt cggaaaagag gcaaacaaat ctggcgttgc tttattcact gatacatctg
      661 tcgcctgggc acttaagatc actgcaatca ataactcaaa tggattattg tgcttgagtt
      721 ctccatgtgc ttcgggaaac attccataca tcgtttctag agcttccatt gttttttgtt
      781 tgcttaacat attccgctac tcctccaacc aatttttcag tgagactttc ggcaattctt
      841 cttgttgagc tgactgttct acttctcttt gaagaagctg ctgttttctt cgtttttggt
      901 cttcttccac ttgttgtttt gtacgtagat tttttctttc ccacgatagc aaaatgcggt
      961 caacgtaatt aaaactataa gcctgattta atacggcttc ccgcagtgct agcttcaaaa
     1021 tttccggctg ataatggtct tcttctagcc attgtccgat ccgctggaac tcgatcgaag
     1081 aaagcgggcg tccaaattct tgttcaaata attgatacac tgactggatt tctttttctt
     1141 gtgttttttc ttcgtatttt ttttcttgtg tttttaaata ctcacctaat agatcataga
     1201 tggggtaaag attatatcga tctgcttttt taccgttgac aatcgtcgtt tctatcttga
     1261 taaatccatt tgttacgaga cgatccagta tttgaaaaat ctgatcttgt ttgattccca
     1321 tatccatagc aatcatctgc aaatcaggga aagtatctcc ttcaagctgt gccatatgca
     1381 actgtagaac aaataaaaat tcttcagatt gtagaccgat cctatgataa tttttaagta
     1441 aaagattaga tatgactgtt tgtccagcgc ttaagtaatt ttctaaattc aacatgattt
     1501 ctcctccgct caatcacagt atacgtaaaa aaaacatggg aagcaagaac aggtttcttt
     1561 tctatttact tcactgatcg gtcagattta caatgtcgtc tacttgattt ttgtttgttc
     1621 atgtttttga tgcgtatctt cacttttcgg aataaataaa ttaagtagat ttcgctgttc
     1681 cttagaatgt tgacaaggaa acaaaattac tatacactat ttttgtttcc aagttaacat
     1741 tattgcataa aggagttttt acatgtacag tttcacaaat aaacattcta aagtttttgg
     1801 atacgtctct gtcttattca ctggattagg atttactatc gtcagtcctg tacttccgtt
     1861 tttaacgctg ccttattctc actccataca tcagcaagct ttctatatca ctttgctcat
     1921 gtctgtttat gctctagctg ccttcttatc tgcccccatt ctcggtagct taagtgatca
     1981 ttttgggcgt catccgatcc ttattatcag tttattagga tcaagtatcg gatatttgct
     2041 ttttggttta ggaaattcga tatggatgtt gttccttggt cgtatcattg aaggattgac
     2101 ggcaggagaa atctccacct tatatgctta ctttgccgac atcacggaac ctaatgaaag
     2161 aaccaaagtt tttggatgga tgggtgcttt agtcggtata ggaacaacgc tgggtcctat
     2221 tatagggggt ctattagctg agttggggaa ttctgttcct atatttattg gagcactctt
     2281 tactttctta aatgccgttt atggctacac atttatgcta gaaagccttc caataaaaaa
     2341 acgttcagta gatttaagtt tctcccatgt aagacctttt caccaattac aacaattatt
     2401 caagatttca tccgttattc ctttattgac agcaggtttt gctgtttggt tggcagctgg
     2461 ctctcttcaa tctatctttt ctcaattttc gattgacgcc tttcagtgga aggctgggtt
     2521 ggttggttta agcttctctt tgattggaat cttggatact gtctcgcaat tgttcattat
     2581 gcctcgattg ctcaaaaaat tttcagaaca gcagatcaca agaataggga tgttcagtga
     2641 aatcctcgcc tatctattca tcacattatc tggaattctt ctactgccga ttttgttttt
     2701 atttgggatt atctgttatg gtttcggtga ttctattttt actcctgtat tcaatggcca
     2761 actatccaat tctgtctcag ataaccagca aggtcttgta atgggcggta cccaaagtat
     2821 acaatctcta tcgcgcgtca tcggcccgct aatcgcagga cagctttatg ctgcagcacc
     2881 atttcttccg acggtattcg gatttttgct tatgattttt gcctattatc actacaataa
     2941 aaaatagtag cttcatttgc tacttcgtat aaattctctg tatcgaccga taaaacgaaa
     3001 tactgtgtca aattcttctt ttgacatatt ttcaaataca gtagcatctc gctgtaccca
     3061 tttttgatgg agttgctcgt gtctattcct tattttttgt ccgattggcg tcaattgaaa
     3121 atagatttct tttttattct cttcgctttg ataagtgttt atgatccctc tagctatcag
     3181 tttttttgtt agcttactga tggctcctct agtcatgtaa ctagcattcg ctaattttgt
     3241 cacgttagga tcagggattt tcccgatatg ttcaatcact tctagttcat ttagagtaaa
     3301 tcctgcaaat tctttttcca tttctggttt attacgatga ataatctcat tagctaaatc
     3361 cagatattct tctattagca gttgtctttt atccatttct acaccttctt tttctttcat
     3421 tatattgttt ttttgtttcc tattcaacaa agataaaaat attttgtagg aaaacccaat
     3481 ctgaaaccaa gaaaaaaagg ggttgacaaa gcttgtcata accccttttt ctgattaaaa
     3541 gatagtaaag agacagtcat cggtaattgt gatgattttt tgtcatcact taaggatgag
     3601 cggccttttc aaaaactgtc tttaaagatc atcatcagtt tctagcacat atggctgacc
     3661 ttcatatgtg tactctgtat tcaatgtgcc gtcattttca tctgagtaga tgcgtctact
     3721 gaaaggaaaa cgatgagaaa cattatcttt ggcgtcattt gctggcatcg tgtaatattc
     3781 ctcttgcgtt tcattcaact gagagagtac ttgatctgcc aactcatcaa tattgatcgt
     3841 taatttcttc gaagcacttt tataatcttc taaaaatgca tcttgttgga caaaatattc
     3901 tttattcatc ttctttctca cctttctttt caaaatcaaa taaagctgct tgttctttta
     3961 tgtaacagct ttatctttgc tgcttattgt gacaaaaaat tcgaagaaag acaactaatt
     4021 gtacttttca caaaaaaaga cagagggtcg tgacaaaatt gtgtcacaac cctttttctt
     4081 tattgtgcag tttcgatttc gcctagcgct gcttgtgcaa gtaggttcaa gtaattccat
     4141 gggcggtcaa aatgtggttg gaagaagaaa tctgaaagtg cgagatcttc tacagtcatt
     4201 ttattttgaa cagctaaaga catggtattt gctgattgtg taatgtcgta tttggacatg
     4261 aactgagcac ctacgatacg attcgttcct ttttcataga ctagctccat cattactttt
     4321 tcagtagttg gcatgaactc tggacgataa ttatcttcaa atacagtcgc ttggacttct
     4381 agatcgttca aagttgcgct ttctttggtc acacctgtcg aaccgatcgt ccagccaaat
     4441 aggtaaagtc ctgaagttcc ttgagtacca cgatacgcca ttttatgatc tgtcaagtta
     4501 tttcccacaa gcaaaccttg acgaacggca tttgttgcca aaggaatata gttttgcgtg
     4561 ccacttggat tgtagttgac tactgcagag tctccagctg caaaaatgtc tggattactt
     4621 gtctgcatgt attcattcac tttgattgcc ccgtttggca gtgtatctac ttttccttcg
     4681 atcagttttg tatttggacg gaagcccaca cataaaatga ccatatctgt atcaaatgta
     4741 tctgttggtg tagctacttg tttcactttt ccttcttcat ctgtgataaa ttcagtaaca
     4801 ttttcgccta gcgcaagagt aacaccgcgg tctgtcaatt ctttttctaa gatatctgtg
     4861 aatggtttat ctagatattt attcaagatg cgatctaatc catcaatcaa tgtcacttgt
     4921 tttccagatt cgacaaaagc ttctactaat tcaataccga tatatccacc accaacaatt
     4981 actacacgtt ttgcttcttt cgcttgtgca ataatttcat ttgcttgatt gtagtttttg
     5041 cataggagta catttttcgc atcgattcca ctaatcggtg ggataatagg ccatgaacca
     5101 gttgtcatca ctaatttatc ataagaaacc gtttctgttt ctcctgtttt cagatctttt
     5161 gccgttactg ttttattttc tgtatcgacg tctgttacat cgtgttccat tttaactgtt
     5221 gctcctaatg aagataattc ttcaggattt gagtagaaca agcctgctgg gtcctttact
     5281 actccgccga cataaagtgc gataccgcaa gataagaaag aaatattgtc atttctttca
     5341 tatactgtca cttctgcttc tggatgattt tttaaaatac tttttacagc tgcagtacct
     5401 gcatgagtac agccaattac tactactttc atttatggtc cctccgatta acattttgtc
     5461 cttcgacaaa actactataa cagaaataaa acggatacaa cagtttaatg cttgtgaatt
     5521 cacaaaggtt tgtttgtagt ttcattcctt ttcaagcata agaacccaat atctacaaga
     5581 ttcttatttt aataatattc acttttatat ctaagattgt gaataaaaga caaaaaaata
     5641 aaaaagctag cgtttttttt acacagctag ctttttttag gtataacact tgtagaaact
     5701 gtttttacat ttgcgccttt tttgctgact ctttacaatc cggacagagt ccatacaatt
     5761 ccaaccgatg ttcattaatc tcaaaatcag tcagtctgct tgcagccatt tctatatctt
     5821 caagccctgg gtaatgaaaa tcaacaatct taccgcagtt ttcacaaatt acatgataat
     5881 gacgctttga actgaaatca aatcggctag aagcatcgcc gtacttcatt tcttgaacaa
     5941 atccaatcga agtaaataag cgaaggttat tgtaaacagt cgcaacactc atatttggaa
     6001 accgatgttc aagtgcttta taaatctcat cagccgttgg atgggaatga tgttcaatca
     6061 aatattctaa aaccgcgtac cgttgaggtg tgattcgaat gttctctttt tttaactttt
     6121 cgataacctc ttcgactaac ttattttcca tgtaaacacc ccacgttgtt attctcgtct
     6181 tatcatacta tttttacatt ttaaaatcca cttaacatga aaagaaaatt caattttatt
     6241 gtattttaat gaaaaaagac gtaaaacagt cgcgttcact attctacgtc tctattcaaa
     6301 taatttttct catcataaat ttttgtgtgt tcttttcatc tttaaaagtt ttttacataa
     6361 acctatctgg agaaaaacca agtgccttat atctcttggt aaagggctgt agataaataa
     6421 cgttcaccat tgtcgggaac cacagctaat accttcttgc cttttcctaa ttcttttgcg
     6481 acttttagcg cggcagcaat tgcagcgcca gatgagatgc ctactaaaat cccttctttt
     6541 cgcccaactt ctctagctgt ttccatcgct tcgtctccac taatagaaag gactttatca
     6601 tacacagaag tatccagtgt tttaggaaca aaacctgtac cgattccttg gattttgtga
     6661 ggtcccgctt ctttcccttc taaaatagca gattctgctg gttctacccc gattaattcg
     6721 attttcggat acacacgttt taattctcgg ccagcgccag tgatcgttcc tcctgtaccg
     6781 atacctgcaa caaacgcgtc caagccattt actccaaatg cttgatgtat ctcaggacca
     6841 gtcgtcttct catgcacaag aggatttgct tcattttcaa attgtaatgg taaaaagtag
     6901 ccgttttctt ttgccaaacg ttctgcttct ctgattgatc cagaaatacc atccgcacca
     6961 ggagtcaaaa tcaactctgc gccgtaccct ttcatcaata aacgtctttc gatactcatc
     7021 gtctcaggca tcacgatgat tactttatat ccttttgcaa ctcctaccat cgacaatccg
     7081 attcctgtat ttccagaagt aggttcaatg atagtatcac ctggtttcag tagaccatcg
     7141 tgttctgctt tttctatcat acttaaagca atacgatctt ttacacttcc tcctggatta
     7201 aaaaactcaa gctttacaaa aacatctgct gaatcttctg gtacgatttt atttaatttg
     7261 acgattggcg ttttgccaat caattcggtg actgagttat agatagctgt catcttcaat
     7321 tcctccttgt ctttgctctt cttacttatc ataatacaat ctgttcattt atggcgagtg
     7381 attcgtcttt gactagcaaa aaaaagacaa agatatacca tatatggtaa t
//
LOCUS       DAJSBN010000095         6643 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJSBN010000095 DAJSBN010000000
VERSION     DAJSBN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.096"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(1..859)
                     /locus_tag="ONT86_002633"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ONT86_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ONT86_002634"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ONT86_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR2353379.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKIFRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ONT86_002635"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ONT86_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2603..3421)
                     /locus_tag="ONT86_002636"
     CDS             complement(2603..3421)
                     /locus_tag="ONT86_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2353380.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ONT86_002637"
     CDS             3582..4271
                     /locus_tag="ONT86_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR2353381.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ONT86_002638"
     CDS             4285..5787
                     /locus_tag="ONT86_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2353382.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ONT86_002639"
     CDS             5800..6276
                     /locus_tag="ONT86_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2353383.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ONT86_002640"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ONT86_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaacaccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatatttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAJSBN010000096         6516 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJSBN010000096 DAJSBN010000000
VERSION     DAJSBN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.097"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(207..1169)
                     /locus_tag="ONT86_002641"
     CDS             complement(207..1169)
                     /locus_tag="ONT86_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2353384.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ONT86_002642"
     CDS             complement(1171..2610)
                     /locus_tag="ONT86_002642"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR2353385.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ONT86_002643"
     CDS             2795..4741
                     /locus_tag="ONT86_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HCR2353386.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="ONT86_002644"
     CDS             5005..5877
                     /locus_tag="ONT86_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2353387.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            6173..>6516
                     /locus_tag="ONT86_002645"
     CDS             6173..>6516
                     /locus_tag="ONT86_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2353388.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTG"
BASE COUNT         2083 a         1136 c         1159 g         2138 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacacgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat
     6121 gttacactca aatcgtaata cccgctaaag catacaagga gagtgaacat aaatgactta
     6181 tacccatctt acaccaaacg agcttgtaat gatagaagca tattttcatc aagaaactcc
     6241 ggttgctatc gttgcgaagc agcttaaacg tggacgccaa acaatttaca atgtctataa
     6301 ctttctcaaa tgtggtggaa cagcacttga atgctttgaa caatacaaag aaaataagcg
     6361 acgttgtggg agaaccgaaa tcatttttcc tgctgaggaa aaagaataca ttgcaaaaag
     6421 atcaactgaa ggttggaccc cagacgtcat tattggccgt gcagagcgaa ccttctcttg
     6481 ttcagtaagt accctctatc gccggtttaa gacggg
//
LOCUS       DAJSBN010000097         6267 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJSBN010000097 DAJSBN010000000
VERSION     DAJSBN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.098"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            639..1550
                     /locus_tag="ONT86_002646"
     CDS             639..1550
                     /locus_tag="ONT86_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HCR2353389.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2037..2303
                     /locus_tag="ONT86_002647"
     CDS             2037..2303
                     /locus_tag="ONT86_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353390.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2375..2716
                     /locus_tag="ONT86_002648"
     CDS             2375..2716
                     /locus_tag="ONT86_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353391.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2771..3190
                     /gene="ssb"
                     /locus_tag="ONT86_002649"
     CDS             2771..3190
                     /gene="ssb"
                     /locus_tag="ONT86_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2353392.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3267..3563
                     /locus_tag="ONT86_002650"
     CDS             3267..3563
                     /locus_tag="ONT86_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353393.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3578..4009
                     /locus_tag="ONT86_002651"
     CDS             3578..4009
                     /locus_tag="ONT86_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR2353394.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4191..4496
                     /locus_tag="ONT86_002652"
     CDS             4191..4496
                     /locus_tag="ONT86_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353395.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            4900..5388
                     /locus_tag="ONT86_002653"
     CDS             4900..5388
                     /locus_tag="ONT86_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2353396.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            5375..>6267
                     /locus_tag="ONT86_002654"
     CDS             5375..>6267
                     /locus_tag="ONT86_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2353397.1"
                     /translation="MEKIKQTFPLINKYWNKKKIFIFFITFLLSSLFSLIFPYLFSNV
                     IDLYLENELSQNIVRIVTILIFQLFLVLIKEVYGYIKTIYAFEVDLSMKIDFLKVLQK
                     KNLFFSQTKHSGEIQFRMFSDISTIVNTIFIFSVELPVIGVLVLVAVFIISKVNLIFL
                     IFSLIMMGAMVLQYNLFQLPLKKTIENTKSVNQSITGELNAHFDQLEIISAYNFENIS
                     ILKFDSRFKELFNVFKKQNKLLTLGSCLAQVIFQLWIGGIFLIDVILLAKGTISITNF
                     VIVFMYANMVPTLWTTLISII"
BASE COUNT         2306 a          883 c         1251 g         1827 t
ORIGIN      
        1 aagtgggggg agggctgtcg gcgcttggta tgaaaggcat tctccgactt ccaggttttt
       61 gaccattttc tctcaacgtg gaaaaaccgc cacttatgag agaaaatggt caaaaaccta
      121 agcgggaagt ctccgaataa cataccaagc gccgacagcc aacagataaa gaatacacta
      181 ctaaaaatag tagtagcata aaatttaaaa gggaaaaaat caacccctac cccagtcttg
      241 gggtctcaaa actaactccc ccctaacccc ctctttcagc ctcttttgaa agagggggag
      301 aatcaacccc acggccaacg ccccagtgtt cttggggctg tccagaacag aaacttgcga
      361 gagagtgggg gtaaaacaag tgtagaggaa acttgaagac ttgttcgaac agaaacttgc
      421 gagagagccg gagtaaaaca agtgtagagg aaacttgaag acttgttcga acagaaactt
      481 gcgagagagt tggagtaaaa caagtgtaga ggaaacttga agacttgttc gaacacaaac
      541 ttacaagaga ggggagtaaa gcagttgcag aggaatcagg aagatttgtt cgaacagaaa
      601 cttgcaagaa agcattatta aattgaaggt gatgaaatat gaagtattta ttgcatggct
      661 attttattga ttattctact cgcaatagta gtactttagc gattctatgt ttgcttcatg
      721 aattgagagt ggttactaga aaacagctat acaaactatt gaattttgac agatcaatta
      781 gtaaacaagg agtaacgaat gctttagtga gattatatga aaaacagtta atcgatcgaa
      841 aaaaatatgg aacagaaatg tattattatt tgacgaaaga gggacatcaa agcataggtg
      901 ggtattatac gttgccgaaa gttcctgaat ataatttgaa tcaccattta caaattaaca
      961 attatttgat taaaggaatg gcattggcaa aagaccaccc acatttaaaa cggattattt
     1021 cagaaagacg taccgtctat gaaacgaagg atttaaatcc taagacaaaa ggaagaaaat
     1081 tttttgtgcc agattttatt tttcgttttc gagacaaaga aaatcatgag atcaattggt
     1141 cctttgaaat tgagctaacg ttgaaaacac gtcgtcgcta ccgtgaagcg atttttccga
     1201 aatatattcg tgagttgcga acacgtcctg aatcacattt aatttatgtg acacctagtg
     1261 gcgcaattca agaagaactt catgcgttta agcgtatttt tttcaagcgc gaaactgaaa
     1321 aagatgcgag tcaacaagaa ttgtttgagc ggttgcatat tttttcagct ttagagtttg
     1381 aagagggatt aaaagcatta ttagagaatg atccagcagt caattggcgg acagtagaac
     1441 gagaaaaaat ttatccgaat ggcgctgttc aaagtcgttt gaatttattt gaagaatcaa
     1501 ctgaaaatga ggaaaaacca aaagtgaagt ttacgcctga agtattgtaa aagagggtgt
     1561 tcgcttcgct ctttttttgt gccagtttgc tggcaccgtt cagacgcaag cgtctgaacc
     1621 ggctcgtttt taggaaggac taacttgttt gatttatccg ttgtcaaaga agtggacgtt
     1681 cgagagtccc taatcgtata gcagtcattc ggcgagtata acctaaaaca taacctgcca
     1741 attgccaagg gtaaaatgca cgctgcgcgc ccttggcaat cgtcagaaca tgttttcgag
     1801 ccggttatgc cgaacgaccg ctatacgaaa agggcctcta aacagaagtc cactttgatc
     1861 cttgacaact gaaacccaaa atcaaacaag caggccttcg tctaaaaacg agccgaatga
     1921 agaccttggg ggttgcaccc ccaaaccccc gtgcccccga cacactaccg aaaaggtggg
     1981 ggtgggtggc gaaaaaatca aataaaacat tctattcaag aaaggaagaa acaaccatga
     2041 caactaaaaa cgagcaactg aaagaacagg caagaaaaga gttgcaagaa agaggtttga
     2101 ttattgaggg gagctttgaa ggagattttg aaacctatat tggttgttat gcaagaccga
     2161 taaacaaacc tacagcctta gacccaatga acgaacaaga agtaaaagaa caagaaaaac
     2221 atgcagtgaa tggttttccg caagatttca ctgaatggta tgagtgggaa attaacaacg
     2281 ggaagcttga aaacttctta taaaagtcag atgacgtgca aagagaatga ttctctttgt
     2341 acggtaaaaa tataaagaaa gaaggaaaaa gaaaatggca aaaaacacac cttatcaagt
     2401 aggagacatt ttttatagta gttggggcta cgaacaaacg aacgtgactt tttggcaaat
     2461 tgtgaagtta acagaaaaaa cagcatggtt tcagcctatt caaaagaaaa cggtcgagca
     2521 ttattcgtcc atgagtggaa aagttgttcc aatgaaagac aagtttatag agtatccttt
     2581 agcaaaaact aaagacagta ttgtaaaaaa acgaattgat ccagaaacct atcctgatat
     2641 gttatgtggt tatcgtagtt gggacacgtt tcatagatat aaaaatcaag ctgtttatga
     2701 aagtagttat tattaagggg atagtttccc ctttcaaaaa taataaatag aagaatggag
     2761 agaaaaaatc atgatgaatt cagtgaattt agtaggacgg ttaacaaaag aagtggattt
     2821 aaaatacaca ccaaaaggca atgcgacagg cacatttatt ttagctgtta atcgaaatta
     2881 tacgaatgca aacggcgagc gtgaagcaga ttatatccgc tgtgtcattt ggcggaaagc
     2941 tgcggaaact ttagcaaaat atactggaaa aggaagtttg attggcatta atggacgtct
     3001 tcaaacgagg agctatcaga atcagcaagg acagaccatg tatgttacgg aagttctagt
     3061 gacagatttc tatttgttag agagtaaaga aatcaatgag caacggtcaa aatcaatggc
     3121 gaataatcaa aataattctt caattccagg aaatgagatt caaatttcag atagttattt
     3181 gcccttttaa gtgaaaaata attttgttct atacctgatt tcttcaggta tagaacaaaa
     3241 aataaataga gatgaggcga ttttttatga aaatcattga cagttacgat caacgaacca
     3301 tgcaatattt gggctcgttt gattatacag atgaaaatat tattaattat gttgcaagtt
     3361 tacctcctta tcaaagcgtt cgcttggtag attttgcaag tgatgaattg gtattaacaa
     3421 cgatgggcaa ctttttagat tatgtcggag acaaaaaatg gttggaaaaa attttgtcta
     3481 aattgattgc gaaacaaaca ggagaagaac ccataaatgc gattcaattg ttcaatcgat
     3541 atgaacaaga aaacaatgat taaagaggag agaaaaaatg aaaagattaa aacgaagtat
     3601 ttttattgtg ctagcattga ttgattttgt tgggacaggt tgtgaagcaa ttgacgttca
     3661 aaaagctccc ttatcggaaa taaaacaagc aataacaaaa caagatgaag aacaaccgct
     3721 aaatacacaa gtagaaatag tgaatcaaaa taaaccgagt ttttctgaag aagatttttc
     3781 gttagaaaat ggaaccaggc aatttttttc agatttggat cgtttaaatc gggtaggtgt
     3841 agcaagtgca cttttgcata aaagcaggat gccaacagaa gaacgagacc gattatacat
     3901 taagccaact ggctggaaac aaaagaaaat gaagaatggc gattatcttt ataatcgttg
     3961 tcatttgatt ggctatcaac gtgcgacaag tttccccata agtatgtaat acgaaacggg
     4021 aggattaatc ttaagattaa tcacaactaa cctatttgac agttggaatg aaagctatca
     4081 tgtctgctga atcaactggt ctgatactcc tacatgcaat aaagtctact aggaagttat
     4141 tgtatgaagc taggtaaagg cggcagaaca atttctaagt gtggaatcat atgaagttgg
     4201 atatgccggg acgctataaa ttactcaggg tgagactgtt tttaattaaa ctgacgaact
     4261 tgcgaatgta cgggtctata accgacttat attcgaaaga tataagattg cgaatgcaaa
     4321 gaacattatc gaaaagtaag aatgttggtc atgaaattcg agatggaaga ctagcggtgt
     4381 atctgtcaag agttggtaaa acatgtttga ttgattcaga tgagagtggg aatgcgacaa
     4441 aacgttttac agaagaaata gagcaactag cagaactgaa tatacgatat ttttaaatac
     4501 tttagcggag aataaaaaaa tcctctacag aaaactacgg agggcaacat gtgctattgt
     4561 ttcaacaata catttttaat ttttaccata acaaaaaagt acttgaaaaa aaacagaaat
     4621 atgttaaatt aaaaatgaaa aaatggaagc ggttacttat tcttttgaat ctaagataaa
     4681 aattcaaaag aataacataa cccaaatatg attatacttg agattctaaa tatttatcga
     4741 aaaggaattt gttttaagga aaaatgattc tatggacggt tattgattta tatttaccaa
     4801 atggctaata aaatagtatt tcttatattg tatagttttt gtttttatgt gaaaacatga
     4861 gcaatagaat tgtactagta ggataaggat ggatggaaaa tgaaaaaaac tatttttgtt
     4921 ttagcgattt ttatatgttt tgggctactt ttggtaggct acttgttgtt taattctaat
     4981 gaaatgagaa ctatttctac gaatgacttg ttaaaaaaaa gtgaattaaa ggataaaaaa
     5041 aaagaatctc ttgcctttat aatgtttact caagaagagt gtgagtattg tatagcaata
     5101 aatccaatca tagatagtgt aaataaggaa actttgaata agataaagaa atatgatata
     5161 tctgtagatt ttcaatctaa acaaaagtgt aaaattaagt ttgttccgac tattgttgca
     5221 tatagtaaga ataaagaagt ggggagaatt gacaatgaat tagataattt gtatcgaaaa
     5281 aagcaagatg gttcaattgt aatagacaag aaaaaaacaa agacattgct tatagattgg
     5341 atgaaaaaaa tggataaaca aggagaatat ttgtatggag aagattaaac agacatttcc
     5401 gttaattaat aaatattgga ataagaaaaa aatttttata ttttttatta cgtttctgct
     5461 gtcatcttta ttttctctaa ttttcccgta tctatttagt aatgttattg atttatattt
     5521 agagaatgag ttatcacaga atattgtgcg aatagtaacg attcttatat ttcagttatt
     5581 tttagtctta attaaagaag tgtatggata tataaaaact atttatgcat ttgaagttga
     5641 tttatcaatg aaaattgatt ttcttaaggt tttacaaaaa aaaaatcttt ttttttcgca
     5701 aacgaagcat tcaggagaaa ttcaatttag gatgttttcg gatatttcta ctatcgtaaa
     5761 tactatcttt attttttctg ttgaattacc tgtgataggc gttttagttc ttgtagcggt
     5821 atttattata agtaaagtga atcttatttt tctgattttt tcactaataa tgatgggagc
     5881 tatggtgtta cagtataatt tatttcaatt accgcttaag aaaacaatcg agaatacaaa
     5941 aagtgttaat caaagtatta caggagaatt gaatgcacat tttgatcaat tagaaattat
     6001 aagcgcatat aattttgaga atatctctat tttaaaattc gattctagat ttaaagaatt
     6061 atttaatgtt ttcaaaaaac aaaataaatt actaacactg ggaagctgtt tggcacaggt
     6121 tattttccaa ttatggattg gaggaatatt tcttattgat gttattttat tagctaaagg
     6181 aacaataagt attactaatt ttgtaatagt atttatgtac gctaacatgg tccctacttt
     6241 atggactact ttaattagta ttatttg
//
LOCUS       DAJSBN010000098         6188 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJSBN010000098 DAJSBN010000000
VERSION     DAJSBN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.099"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            87..1094
                     /locus_tag="ONT86_002655"
     CDS             87..1094
                     /locus_tag="ONT86_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2353398.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1106..2110
                     /gene="neuB"
                     /locus_tag="ONT86_002656"
     CDS             1106..2110
                     /gene="neuB"
                     /locus_tag="ONT86_002656"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HCR2353399.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2107..3255
                     /gene="neuC"
                     /locus_tag="ONT86_002657"
     CDS             2107..3255
                     /gene="neuC"
                     /locus_tag="ONT86_002657"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HCR2353400.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3228..3905
                     /locus_tag="ONT86_002658"
     CDS             3228..3905
                     /locus_tag="ONT86_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR2353401.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3895..5037
                     /locus_tag="ONT86_002659"
     CDS             3895..5037
                     /locus_tag="ONT86_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR2353402.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            5050..6027
                     /locus_tag="ONT86_002660"
     CDS             5050..6027
                     /locus_tag="ONT86_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2353403.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT         2313 a          775 c         1191 g         1909 t
ORIGIN      
        1 cattattgat aattagttta gttatatatt ttttaaagat taagaatcga attatagatg
       61 actataattc ataggaggag atataagtgt ttaaaggaaa aacaatatta gtaactggcg
      121 gaactggttc cattggaagt gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta
      181 tcagaattta tagtagaggt gaagaaaaac aattttatat gcaacaagag ctctcaaatc
      241 acactaattt gagattttta attggtgata tcagagactc tcaaaggatt gattatgcat
      301 gtcgtgatgt ggatattatt tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat
      361 ataatcctat ggaagctgta aaaacaaatg tgatgggaac tcaaaatgta atagatgcag
      421 cgatcaaaaa taatgtaaaa cattttgtag gtatcagtac agataaagta gtgagtccta
      481 ctaacaccat gggtgcgaca aagttattga gtgaaaaatt aataatagct gccaatcagt
      541 ataaaggaaa aacagaaaca atattttctt gtgtaaggtt tggaaatgtc atggggtcaa
      601 gtggatctgt tattcctctt tttagtaaac aaataaaaaa caataaagat attactttga
      661 cagatgttga tatgacacga tttatgatgt caataccgga tgctgcaaga ttagttctgt
      721 ctgctgctat gaattcaaaa tttggagaaa catatgtctt aaaaatgccg gcaatcaata
      781 ttttaacttt agctcaatca ttaataaaaa tatataatga tacacatgat gaaaaatata
      841 gaggtgaaat aaaagttgta ggtatcaggc caggagaaaa attatatgaa gagctaatga
      901 ctacagacga aattgaacgc tcatatgaaa atgataaaat gtatatcatt ccaccacact
      961 atcatgctac tgacgaactg tacaaagatt atgaaaaaat taaaatcact gcatactctt
     1021 cagatgcaac agaattaatg agtggacagg aatttattag aatcgcaaaa gagaataatg
     1081 tcgtgacttg gtaaggagtt ttatgatgaa atcagaagta tatgtaattg cagaaattgg
     1141 tgtaaaccat aatggaagtc tagatttagc tttagaaagt attgataaag ctaaagaatg
     1201 tggagctaac gcggttaaat ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc
     1261 tccaatggca aaatatcaga aggataatct taaagaggac agttctcaat ttgaaatgct
     1321 aaaaaaatta gaattatctc aatctgattt tattagaata aaagattatt gtatacaaaa
     1381 taaaattgat tttttgtcta ctccttttga tgaagaaagt gcagactttc taaaacaaat
     1441 tggtgtacaa ggatttaaaa ttggctcagg agatttaaca aatttacctt ttcttagaaa
     1501 aatagatatg tatgggttgc cagttttatt atctacaggt atggctgata ttcatgaggt
     1561 cagtgaggct gtagattgtt tcatagatag tccagttact attttacatt gtacttcaaa
     1621 ttatccggct agttctgaag atattaattt attggcgatt aacacattaa aagaaatgtt
     1681 cgggataccc gttgggtact ccgatcattc attgggatat gatgttggta tttgtgctgt
     1741 tgcgatgggt gctaaggtaa ttgagaaaca ttttaccttg gataaggaac tacctggtcc
     1801 agatcataaa gcttctttag atcctcagga atttgcagat tttgtaaagc atattagaaa
     1861 cactgaaata tttttaggtg atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa
     1921 agaagtatca agaaaaagcc tagtggtcac tgagaattta aatgtgggag atattcttac
     1981 agagaaatct cttacaataa aacgtcctgg aaatggaata gctcctaagt ttttatatca
     2041 atatattggc aaaaaagtta aaagaccaat aagtgcagat agtgttctgc ttgaggaaga
     2101 tgttgaatga aaaaaatact tatagtaagt ggaacacgag ctgattatgg aatatataaa
     2161 cctgttgctg agaatttact acaagataat tgggatgtat catttgttgt ttctggaatg
     2221 catttatccc ataaatatgg atttactaaa gaattgatct tagctgacgg atttagaatt
     2281 agtggagaag ttcaaagttt gtttttagag aacaataagg gaaacatggc tcggtctgtg
     2341 gcattagaga taaatggttt cacaaatata tttgaacaaa ctagccctga ttttgtatta
     2401 ttactagggg atagaggaga aatgttggca gctgcgatag cttgtttgta tcttggaatc
     2461 aaaacagcac atattcacgg aggcgaagtg tctggtactg tagatgaatc aataagacat
     2521 gccataacga aaatagcatc tgttcatttt gctgctacgg aaaaaagcaa agagcgttta
     2581 ataaaaatgg gggaagattc ttggagagtt tttcccgtcg gagctcctag aatagatact
     2641 attttaaata atagattacc tagttttaga gatataaaag aaaaatatca tctaaaattt
     2701 gaaaagaaaa aatacgtttt gcaagtattt catccagttg taaccgaact agaaaatata
     2761 gaaaaacaaa ttttagcttt gatcaattca atggaacaac aatctcaacc aattatttgc
     2821 attttaccta atagtgatgc tggtagcgaa attatcagaa aaatgtacca gaaatattta
     2881 aaaacggata ttatttatat tacgaattta gagccggagg aatacctgac tatattaaaa
     2941 gagtgcaaat ttatgatcgg aaattcttct tctgggataa ttgaagctgc atcattcaaa
     3001 ataccagtaa taaatttagg aaccagacaa aatggacgag aacaatctaa aaatattata
     3061 aatgcaaatc taaatgaaaa tgaaattgaa actgctttga ataaaataca agatcctcag
     3121 tttataaaaa gcttgaaaaa tgttaagaac atctacggtg acggcaacag ttctagaaga
     3181 ataacaaaaa cattgaatca aatattgaat gatggggaag atattaagtg gattcaaaaa
     3241 caaatagctt attaaaatat actagtgatc catacattat tattggggct ggcggacacg
     3301 caaaagtcat tattgaaacc ttaagattag aaaataagtt aatatatgga ttttttgatg
     3361 ataagcaaaa taaaatgcaa gatctgaaat atcttgggaa aataaacgaa ttgaatagct
     3421 gggcggatag caaaaaaaaa atctataaat ttattgtagc tattggtgac tgtgtagtaa
     3481 gaaaaaagat tataaaaaaa ttgaatatgc caagtgagca atatggaata gctgttcatc
     3541 caagtgctat agtggcaaaa gaaatttcaa ttggttatgg aactgttatt tttgcgaatg
     3601 ctgtaattaa cccagatgct gtaattggag agcatgctat aataaacaca ggtagcattg
     3661 ttgagcatga ttgtagaatt aataactatg ttcatttatc tccaggtgta tgcttagcgg
     3721 gtggagtaca tgtaggtgta ggaactcaaa taggaattgg ttctcaatgt atccagatga
     3781 agaaaattgg atcttggtgt atgataggcg cagggagcac tattgttaag gatattccct
     3841 cacattcctt agcttatggc aatccagcta aaattaagaa agaaggaatt aattttgaat
     3901 tctagaattt tattatcaag tcctcatatg ggaggaacag aagaaaaata tgttgctaag
     3961 gcttttaaag ataattggat agcgccatta ggtgaaaatg tggataaatt tgaagaaagt
     4021 attacaaaat atactggttc taagggcgga gcttctgcgt taagttcagg tacagctgcc
     4081 atacatcttg ccttaagact gcttaatgtt ggaaaaaacg atattgtctt tgctccttca
     4141 tttactttta tagctacagt aaatcctatt ttatatcaag gggctgtacc agtgttcatt
     4201 gactcagaac ctaatacatg gaatatgagc cctgttgcac tagagagcgc attagaagaa
     4261 gctgaaagaa acaaccaatt acctaaggca atcatcgtag tgaatttata tggtcaaatg
     4321 gctgattatg aacgaatttt agctattgca aaccaattta atgttccagt gatagaagat
     4381 gcagcggaat cgttaggttc tacttataag ggaatttcgt caggcttatt tgggacaatt
     4441 gggatctatt catttaacgg taataagatc atcacaacct caggtggagg agctctagtg
     4501 tctagtgata aaaaatatgt tgagcattca agatttttag caacacaagc aagagatcaa
     4561 gcaccgtatt atcaacatag cgaaataggt tataattacc gtctaagcaa tatttctgcc
     4621 ggtatcggac gtggacaaat ggaagtattg aatgatagag tagctaaacg aagagaaatt
     4681 ttcaatcgat ataaggaagc tttttctgga attgatagaa ttgaaatgat gccagaagtg
     4741 gatggcatgg tgccaaatcg ttggttgtct actttaacga tcaatgatta taattctatt
     4801 gatcaagtag ttaacgtaat aaataaacta aatgaaaaaa acattgaaac aagagctttg
     4861 tggaaaccac tacatttgca accagtattt caaggggtaa aaatgtatta ccatgataat
     4921 aatcttagtg tgtcagaaaa gttatttaat agcggcttat gcttaccttc tggaagtaat
     4981 atgacagata gtgaacaaga atttgttatt gatgagttta aaaaaatatt caagtaggtg
     5041 tgatatttta tgaacatagg aattattggt ttaggttcca tggggaaaag aagacttaga
     5101 ctcattcaag aacactttaa tgatatttta ctatgtggtg ttgatactaa tgaggcgaga
     5161 cgaaaggaag ttgaaaagga atttggaata ccaacttata atagtctaac agaggcaaaa
     5221 aatttgtttg aaataaattc agtttttgtt tgtacatccc ctataaccca tgaatttatt
     5281 ataaaagagg ctttaaattt agattgtaat atctttacgg aaatcaattt attaaatgaa
     5341 tattatgatg aaataatcga agtagctaaa aagaaagata gattacttta tctatcttct
     5401 acatttttga agagaaaaga aattcaatat attaagtcta aagtgacgca taataaaaaa
     5461 atttcttatc aataccatgt aggacagtat ttacccgact ggcatccttg ggaagattat
     5521 cgcaattttt ttgtttcaaa caaaaaaact aatgggtgta gagaaatatt cgctattgaa
     5581 ttaccttgga ttatagatac atttgggaaa gttaaatcgt ttactgtaat gacccagaaa
     5641 ttatccaatc ttgatattga ttattcggac agtttcactc ttattattga gcatgaaact
     5701 ggtatagttg gaactttaag tgtagatgtt gtttcaagag tagctaaaag aaatttaact
     5761 ataattggtg aaaagatttt aatagaatgg gaaggtaacc cggattctct attttcatgg
     5821 aatgaagaaa cttctagtat gacagatatt caattgtacg atgaggttat gcatgataat
     5881 aattattcac atactattat tgaagatgca tatttagagg agattaaaga atttttcacg
     5941 cttttggaaa agaaaatgga accccaatat acttttgaaa aagataagta tattattaac
     6001 ttaattaacg acattgaggt aaattaatga tgaaagtaat tttttttggg ttagggtcaa
     6061 ttggaaagag acatctaaaa aatctaataa gctatggaaa aaagaattct attaattttg
     6121 atataacagc ttatagtagc agtggaaaaa aagagtttga agaaataacg tatattcaaa
     6181 atagagac
//
LOCUS       DAJSBN010000099         6002 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJSBN010000099 DAJSBN010000000
VERSION     DAJSBN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.100"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..395
                     /locus_tag="ONT86_002661"
     CDS             <1..395
                     /locus_tag="ONT86_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353404.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ONT86_002662"
     CDS             398..1228
                     /locus_tag="ONT86_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCR2353405.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ONT86_002663"
     CDS             1244..2095
                     /locus_tag="ONT86_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2353406.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ONT86_002664"
     CDS             2092..2451
                     /locus_tag="ONT86_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HCR2353407.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ONT86_002665"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ONT86_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ONT86_002666"
     CDS             2624..2929
                     /locus_tag="ONT86_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353408.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ONT86_002667"
     CDS             2929..3240
                     /locus_tag="ONT86_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HCR2353409.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ONT86_002668"
     CDS             3237..3410
                     /locus_tag="ONT86_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353410.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ONT86_002669"
     CDS             3407..3823
                     /locus_tag="ONT86_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HCR2353411.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ONT86_002670"
     CDS             3823..4491
                     /locus_tag="ONT86_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCR2353412.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ONT86_002671"
     CDS             4488..4865
                     /locus_tag="ONT86_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353413.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ONT86_002672"
     CDS             4862..5161
                     /locus_tag="ONT86_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353414.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ONT86_002673"
     CDS             5151..5309
                     /locus_tag="ONT86_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353415.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ONT86_002674"
     CDS             5403..5819
                     /locus_tag="ONT86_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR2353416.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DAJSBN010000100         5793 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJSBN010000100 DAJSBN010000000
VERSION     DAJSBN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.102"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..988)
                     /locus_tag="ONT86_002675"
     CDS             complement(<1..988)
                     /locus_tag="ONT86_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HCR2353417.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGN"
     gene            1140..1835
                     /gene="vanR"
                     /locus_tag="ONT86_002676"
     CDS             1140..1835
                     /gene="vanR"
                     /locus_tag="ONT86_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR2353418.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1813..2967
                     /locus_tag="ONT86_002677"
     CDS             1813..2967
                     /locus_tag="ONT86_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR2353419.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3182..4150
                     /locus_tag="ONT86_002678"
     CDS             3182..4150
                     /locus_tag="ONT86_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR2353420.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4143..5174
                     /gene="vanA"
                     /locus_tag="ONT86_002679"
     CDS             4143..5174
                     /gene="vanA"
                     /locus_tag="ONT86_002679"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR2353421.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5180..5788
                     /locus_tag="ONT86_002680"
     CDS             5180..5788
                     /locus_tag="ONT86_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCR2353422.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1745 a         1131 c         1310 g         1607 t
ORIGIN      
        1 tatttccgat aaccgtgtat tgaaaggcat cttcgaagcc attatccaat atttcgcagg
       61 cttttgtata tttaggctgg tcgatatatt caccgactaa ggcattctta gctgttcgag
      121 cgagttcaat atccgtaaac ttaaagatcg cttttactgc ttctctaaac ggttttgaat
      181 tcttttttgg aatggaactg aagatgtttc ttaaaaaatg gacctggcat ctctgccaac
      241 ttgcgttggt aaatgactta cgaatcgcag acactaggcc tttatgggca tcagaaatga
      301 tgagttctgt cccctttagg ccgcgttctt ttaagtattc aaagaagatg gaccatgtgt
      361 catcactttc ttcattttga atcatgaagc caatgatttc acggtcgcca ccttctgtta
      421 tcccgatcgc aatatggcag cttttagaaa gcactcgatg gtcctcacgg acttttatgt
      481 agagaacatc agtcatcaga taaggataat tcgtacctga gagtgaacgg ttctgccatt
      541 cgttgaccat cgggtctaac tgctcagtca ggctagaaac aaaagatttc gatacagatt
      601 ttccacatag ctcttcaaca atctttgaaa ctttacgtgt cgaaacgccc gaaacataca
      661 tctcaagcat tgaagcgagc agtgcctttt catttcgctg ataacgctca aacaccgtcg
      721 gtgaaaattc accatcacgt gttctaggca cttttaattc gagtgtaccc acacgagtcg
      781 taaagtctcg ctcataatag ccatttcttt gactctgacg actttctgaa cgttcatagt
      841 catcggcttg aatatattct gttcgttgat tttccatcaa ttggttgaaa acagtggtta
      901 aaatattttt agaaacatca tcttttaccg aatgttcaat aatactttga acctcttcgt
      961 tgttcagtgt aaaatgtact tgggtcatgt aacgtcctcc tgggtatgtt tttgtggtta
     1021 aaaacattgt accgtaaaag ggctgttaca tggcctttta tcttttacac aattatatgg
     1081 actttatcct ataatctgat aaattattaa gggcaaactt atgtgaaagg gtgataacta
     1141 tgagcgataa aatacttatt gtggatgatg aacatgaaat tgccgatttg gttgaattat
     1201 acttaaaaaa cgagaattat acggttttca aatactatac cgccaaagaa gcattggaat
     1261 gtatagacaa gtctgagatt gaccttgcca tattggacat catgcttccc ggcacaagcg
     1321 gccttactat ctgtcaaaaa ataagggaca agcacaccta tccgattatc atgctgaccg
     1381 ggaaagatac agaggtagat aaaattacag ggttaacaat cggcgcggat gattatataa
     1441 cgaagccctt tcgcccactg gagttaattg ctcgggtaaa ggcccagttg cgccgataca
     1501 aaaaattcag tggagtaaag gagcagaacg aaaatgttat cgtccactcc ggccttgtca
     1561 ttaatgttaa cacccatgag tgttatctga acgagaagca gttatccctt actcccaccg
     1621 agttttcaat actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc
     1681 tatttcatga gatatggggc gacgaatatt tcagcaagag caacaacacc atcaccgtgc
     1741 atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa tatataaaaa
     1801 cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc caaactagaa
     1861 cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt cgtgttgtat
     1921 attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt ggaaaacaaa
     1981 tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg gaacaatata
     2041 gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg cgtcatgctt
     2101 tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact tattcagaac
     2161 gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa gctcaacaca
     2221 ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat
     2281 gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat tatcggttat
     2341 ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat
     2401 atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt tgagattaca
     2461 cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata ctatatgctg
     2521 gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca ggcggttatt
     2581 cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag agtctttaac
     2641 aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga cattaccgcg
     2701 ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat
     2761 aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc ttccgatacg
     2821 ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca tggagggcag
     2881 atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc agcgatgcca
     2941 gacttggttg ataaaaggag gtcctaagag atgtatataa ttttttagga aaatctcaag
     3001 gttatcttta ctttttctta ggaaattaac aatttaatat taagaaacgg ctcgttctta
     3061 cacggtagac ttaataccgt aagaacgagc cgttttcgtt cttcagagaa agatttgaca
     3121 agattaccat tggcatcccc gttttatttg gtgcctttca cagaaagggt tggtcttaat
     3181 tatgaataac atcggcatta ctgtttatgg atgtgagcag gatgaggcag atgcattcca
     3241 tgctctttcg cctcgctttg gcgttatggc aacgataatt aacgccaacg tgtcggaatc
     3301 caacgccaaa tccgcgcctt tcaatcaatg tatcagtgtg ggacataaat cagagatttc
     3361 cgcctctatt cttcttgcgc tgaagagagc cggtgtgaaa tatatttcta cccgaagcat
     3421 cggctgcaat catatagata caactgctgc taagagaatg ggcatcactg tcgacaatgt
     3481 ggcgtactcg ccggatagcg ttgccgatta tactatgatg ctaattctta tggcagtacg
     3541 caacgtaaaa tcgattgtgc gctctgtgga aaaacatgat ttcaggttgg acagcgaccg
     3601 tggcaaggta ctcagcgaca tgacagttgg tgtggtggga acgggccaga taggcaaagc
     3661 ggttattgag cggctgcgag gatttggatg taaagtgttg gcttatagtc gcagccgaag
     3721 tatagaggta aactatgtac cgtttgatga gttgctgcaa aatagcgata tcgttacgct
     3781 tcatgtgccg ctcaatacgg atacgcacta tattatcagc cacgaacaaa tacagagaat
     3841 gaagcaagga gcatttctta tcaatactgg gcgcggtcca cttgtagata cctatgagtt
     3901 ggttaaagca ttagaaaacg ggaaactggg cggtgccgca ttggatgtat tggaaggaga
     3961 ggaagagttt ttctactctg attgcaccca aaaaccaatt gataatcaat ttttacttaa
     4021 acttcaaaga atgcctaacg tgataatcac accgcatacg gcctattata ccgagcaagc
     4081 gttgcgtgat accgttgaaa aaaccattaa aaactgtttg gattttgaaa ggagacagga
     4141 gcatgaatag aataaaagtt gcaatactgt ttgggggttg ctcagaggag catgacgtat
     4201 cggtaaaatc tgcaatagag atagccgcta acattaataa agaaaaatac gagccgttat
     4261 acattggaat tacgaaatct ggtgtatgga aaatgtgcga aaaaccttgc gcggaatggg
     4321 aaaacgacaa ttgctattca gctgtactct cgccggataa aaaaatgcac ggattacttg
     4381 ttaaaaagaa ccatgaatat gaaatcaacc atgttgatgt agcattttca gctttgcatg
     4441 gcaagtcagg tgaagatgga tccatacaag gtctgtttga attgtccggt atcccttttg
     4501 taggctgcga tattcaaagc tcagcaattt gtatggacaa atcgttgaca tacatcgttg
     4561 cgaaaaatgc tgggatagct actcccgcct tttgggttat taataaagat gataggccgg
     4621 tggcagctac gtttacctat cctgtttttg ttaagccggc gcgttcaggc tcatccttcg
     4681 gtgtgaaaaa agtcaatagc gcggacgaat tggactacgc aattgaatcg gcaagacaat
     4741 atgacagcaa aatcttaatt gagcaggctg tttcgggctg tgaggtcggt tgtgcggtat
     4801 tgggaaacag tgccgcgtta gttgttggcg aggtggacca aatcaggctg cagtacggaa
     4861 tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc tgaaaacgca gttataaccg
     4921 ttcccgcaga cctttcagca gaggagcgag gacggataca ggaaacggca aaaaaaatat
     4981 ataaagcgct cggctgtaga ggtctagccc gtgtggatat gtttttacaa gataacggcc
     5041 gcattgtact gaacgaagtc aatactctgc ccggtttcac gtcatacagt cgttatcccc
     5101 gtatgatggc cgctgcaggt attgcacttc ccgaactgat tgaccgcttg atcgtattag
     5161 cgttaaaggg gtgataagca tggaaatagg atttactttt ttagatgaaa tagtacacgg
     5221 tgttcgttgg gacgctaaat atgccacttg ggataatttc accggaaaac cggttgacgg
     5281 ttatgaagta aatcgcattg tagggacata cgagttggct gaatcgcttt tgaaggcaaa
     5341 agaactggct gctacccaag ggtacggatt gcttctatgg gacggttacc gtcctaagcg
     5401 tgctgtaaac tgttttatgc aatgggctgc acagccggaa aataacctga caaaggaaag
     5461 ttattatccc aatattgacc gaactgagat gatttcaaaa ggatacgtgg cttcaaaatc
     5521 aagccatagc cgcggcagtg ccattgatct tacgctttat cgattagaca cgggtgagct
     5581 tgtaccaatg gggagccgat ttgattttat ggatgaacgc tctcatcatg cggcaaatgg
     5641 aatatcatgc aatgaagcgc aaaatcgcag acgtttgcgc tccatcatgg aaaacagtgg
     5701 gtttgaagca tatagcctcg aatggtggca ctatgtatta agagacgaac cataccccaa
     5761 tagctatttt gatttccccg ttaaataaac ttt
//
LOCUS       DAJSBN010000101         5790 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJSBN010000101 DAJSBN010000000
VERSION     DAJSBN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.103"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(99..1229)
                     /locus_tag="ONT86_002681"
     CDS             complement(99..1229)
                     /locus_tag="ONT86_002681"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR2353423.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="ONT86_002682"
     CDS             complement(1877..3079)
                     /locus_tag="ONT86_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2353424.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="ONT86_002683"
     CDS             complement(3139..3342)
                     /locus_tag="ONT86_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCR2353425.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="ONT86_002684"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="ONT86_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="ONT86_002685"
     CDS             4879..5691
                     /locus_tag="ONT86_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2353426.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DAJSBN010000102         5759 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJSBN010000102 DAJSBN010000000
VERSION     DAJSBN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.104"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1389
                     /gene="gadC"
                     /locus_tag="ONT86_002686"
     CDS             <1..1389
                     /gene="gadC"
                     /locus_tag="ONT86_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HCR2353427.1"
                     /translation="GGILWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAI
                     FFQWFQITVGFITMIYFILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTN
                     RTAKIAKAGFVIGIVIPSIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFV
                     SFILAYMGVEASASHINELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLS
                     LSAGVIQTFETLILHFNSHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGL
                     LPVFFRKTNKQDVPVPLIMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGY
                     LLFFLGYFVLVFKKKDLKRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAAS
                     EHRTYQIILLISFVVTVLLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEH
                     HVTKNDEHILKH"
     gene            1402..2802
                     /locus_tag="ONT86_002687"
     CDS             1402..2802
                     /locus_tag="ONT86_002687"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR2353428.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(2967..4407)
                     /locus_tag="ONT86_002688"
                     /pseudo
     CDS             complement(2967..4407)
                     /locus_tag="ONT86_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            4800..5393
                     /locus_tag="ONT86_002689"
                     /pseudo
     CDS             4800..5393
                     /locus_tag="ONT86_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
     gene            complement(5377..>5759)
                     /locus_tag="ONT86_002690"
     CDS             complement(5377..>5759)
                     /locus_tag="ONT86_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2353429.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
BASE COUNT         1822 a          957 c         1102 g         1878 t
ORIGIN      
        1 ggaggtattt tatggtttct accagttgct ttatgtgctg cagaaatggc aacagttgaa
       61 ggatggaaag atggtgggat tttcagttgg gttagtcaaa ctttaggaga acgttttggt
      121 tttgcagcta tttttttcca atggtttcaa attacagtag gttttattac aatgatctac
      181 ttcatactag gagcgctgtc gtatgtaatt aattttccag agcttaatac agatccattt
      241 atcaagttta ttggtgtatt agtcattttc tgggtactga ctttttcaca gttgggcggg
      301 accaatcgta ccgctaaaat tgctaaagca ggatttgtca ttggaattgt tattccttcg
      361 atcattctct ttggtttagc agcagcctac tttatcggcg gtaatccgat tcaaatggct
      421 attaatgcga aagcttttat tcctgacttt tctcagcttt caactttagt agtatttgta
      481 tcttttatat tggcttatat gggcgtagaa gcttcagcat cacatattaa tgagctggaa
      541 aatccccaaa gaaactaccc attggcaatg tttttactag tgattttggc catcgcctta
      601 gatgcaatcg gcggtttctc agtagcagcg gttattccac aacatgactt gtctctcagt
      661 gctggtgtaa tccaaacatt tgaaacgctt attttacatt ttaacagtca tttacattgg
      721 ttggtaaaag tgattgctct catgattgcc tgtggcgtta tgggtgaagt tagttcttgg
      781 gtagttggtc catcacgagg gatgtttgca gctgctcagc gaggtctgtt accagttttc
      841 ttccggaaaa ctaataaaca agacgttcca gttccgttga ttatgataca aggaattatc
      901 gttactattt gggcagcagt cttaactttt ggtggaggag gcaacaattt atcattctta
      961 gtagcaatct ccttaactgt tgtcatttat ctcgtaggat atttactatt tttcctaggt
     1021 tactttgtat tagtttttaa gaaaaaagac ttaaaacgaa cttacaatgt accaggaaaa
     1081 actgttggca aaacgattat cggtggaatt ggttttattt tatcagtgtt cgcattgctt
     1141 atctcttttg taccaccagc ttcaattgca gctagtgagc accgaacata ccaaatcata
     1201 ctacttatta gctttgtagt gactgttctt ttacctttta tcatttatga agtacatgat
     1261 aaaagtgctc acacaactat tgaggaacct actcacgtga aagctgaaga cgttaatccc
     1321 gctgtttatc cagctgctag aggagagcac cacgttacaa aaaatgatga acatatttta
     1381 aaacattagg aggaattcat tatgttatac ggaaaagata atcaagaaga aaaaaactat
     1441 ttggaaccaa tttttggctc tgcaagtgag gatgttgact tgcctaaata taagttaaac
     1501 aaagaatcca ttgaaccacg aattgcttat caattagtac aagacgagat gttggatgaa
     1561 ggaaatgcgc gattaaactt agctactttt tgtcaaacgt atatggaacc tgaagcagtg
     1621 aaattgatga cccaaacgtt agaaaaaaat gcaattgata aatcagaata cccacgaaca
     1681 acggaaattg aaaaccgctg tgtaaatatg attgctgatt tatggcatgc tccaaataat
     1741 gaaaaattca tgggaacttc aacgatcggc tcttcagaag cctgcatgct gggtggtatg
     1801 gccatgaaat ttgcttggcg taaacgtgct gaaaaattag gtcttgatat tcaagcaaaa
     1861 aaacctaacc tggtgatctc ttctggttac caagtttgtt gggaaaaatt ctgtgtatat
     1921 tgggatgtgg aactgagaga agtcccaatg gatgaaaaac atatgtcaat taatctagat
     1981 actgtcatgg attatgtgga tgagtacaca attggtattg taggtattat gggtattact
     2041 tacactggtc gttatgatga tatcaagggt ctgaatgatt tagttgaagc tcacaataaa
     2101 caaactgact ataaagtata cattcatgtt gacgctgcat cgggtggctt ttatgcacca
     2161 tttactgaac ctgatctagt ttgggatttt caattgaaaa atgttatctc aattaattct
     2221 tcaggtcaca aatatggttt ggtatatcca ggtgtgggtt gggtcttatg gcgtgaccaa
     2281 caatacttac cagaagaatt agtatttaaa gttagttact taggtggaga aatgccaact
     2341 atggctatca acttctctca tagtgcagca caactaattg ggcaatacta caactttgta
     2401 cgctatggct ttgatggtta tcgtgatatt caccaaagaa ctcatgatgt tgctgtttat
     2461 ttagccaaag agatcgaaaa aactggtatt tttgaaatca ttaatgatgg atcagaattg
     2521 ccagttgtgt gctataagct gaaagaagat cccaatcgcg aatggacact atatgattta
     2581 tctgatcgtc tgttaatgaa gggatggcaa gtcccagcct acccattacc taaagacttg
     2641 gatcaattaa ttattcaacg cttagttgtt cgagcagact ttggaatgaa catggctggt
     2701 gattatgtac aagatatgaa ccaagcaatt gaagagttga ataaagctca tattgtttat
     2761 cataaaaaac aggatgtgaa aacgtacggc tttactcact aaaattcttc agagaaatat
     2821 aaaagaagtt aattaactaa taaaaggagt tgtgattacc acacagctcc ttaaatctat
     2881 ttgaataaat atatgatatt catttgttac actctcaatc ttagctgttg ttctattcat
     2941 cattactctt attgtaagta gttcagtcaa atttgcttgt tcaattgatc gataacagta
     3001 tgaattgttt gaaaagtaat cggatatata attgttttct cactacctaa gccttcttgg
     3061 tataaagaac tatcgcaaag tataatccgc tgagtacaaa agaaagtgtc gtctactatt
     3121 tctctaagtt gtttacaaat gatattacta atagaaaagt gttctcccaa aatgtcttct
     3181 aactgttgtc gatagtataa atacctaaag tctgacggag ctactatgaa cacttcaatt
     3241 ggagatttcc gtttatgctc atttaaaaga cttaaactaa tggttagttt acgaatagaa
     3301 ttctcattcc aacgcagaaa taaattgttt tgttttcgcc attgttcaag aatttcatga
     3361 acttctaagt acaaagcgcg ttgctcttca tttaacaagt aagtcttatc aggttgaaat
     3421 atctgaatat cagcccaaag tgagcggata aacgggagaa gtgctttatc aaaaagtatc
     3481 tgattttctt cagatatatt cagagataag ataagttttt gactgagctc aatcaccaaa
     3541 gggttatctg ctacatggtt ttgataaaca acgttcaagt ctttttgtaa caattcaaag
     3601 ttatcgcata gataatttct agagttgaat agaaagtaga tataaaggca ttcatcttcg
     3661 tcatgacata aagagaaaga aagttgctct aatcctttct gtattagctg aaacaaggga
     3721 gtatgtcggg caactctttt ttcttcctga ctaaaatata ttgggttagt cttagatctc
     3781 tgcaaaccga tagcgatacc taataaaata aattgttttt caactgtagg gtaacttctt
     3841 gaaaaaaaat gtttttctac ataatcaatc aattgttgac cgctttgaat aattgattta
     3901 tttagctgga tcaactctgt ccgatttgtc tcatagatta tcgttagtaa caaacttcga
     3961 taatcctttt caggaatttc aaaatggcca tcagcattta agtactgcat ttgtttaaaa
     4021 aaattaatca actcatttcg taagttgtaa actttgctta acgaaatgaa ggtagtatca
     4081 gctatattac tccaattgtg ttcccctgat aaataatgtc ctagtacatt gaaaaagtca
     4141 gagcgctgat agatgagttg agtgagtgcc aacctcgaaa acgaagagtc gaatgcgact
     4201 gtaaagctac ttctagattt ctccactgaa ataatttgct gatggagtat gtcggtaatg
     4261 cattccaagt cgttagtgac agacagtgag tgggcaaacg ttcaattgat ctgctatttc
     4321 gcgaatctga atggtccctt tttcgtgtaa ttgctcacaa atgtaaactt gacgaaaaat
     4381 acttttctcc agataggttt ccaacatagt actcctccta gtcattttat acataactat
     4441 tataactcta ccaatcggaa tagtcctgta atatattgaa tcaaaaatat aaattataaa
     4501 ataattagat ggggtatttg gtttaagtat cccttatata gtaataaatt tataggtgtg
     4561 attctcgaat taatacccga aaatgtgaga gaatttttat ggatttttta ggatacaaat
     4621 taattatttt tgcttttatc ctgattacca gtccagtact gtcggtatcc cattttgcag
     4681 caaaatcatg ttgataaagt gacaagaaga ctgatgagat atattatcca aattgagaag
     4741 ataattaatc ggattcttga gtatacccta ctttttaaga aagtagggta tacaagcgta
     4801 tgaataaaga cataactttc cctgtgatgg acgttcggct tgttccaatt accaaaatac
     4861 aggcgaacga ttataatccc aatcgagtag cgtctccgga aatgaactta ttggagcttt
     4921 ctatcattga agatggcttt actcaaccat tagtttgtta ttacaaggaa aaagaagaga
     4981 agtatatctt ggttgatgtg cttcataggt atcgtgttgc taaggaaaga ctcaacttaa
     5041 aagttgtacc ggtaacagtt attgataaac ccatataaga acgtatggct tcaacaattc
     5101 gacacaatcg tgcaagaggg acccatgaaa taagaggaat ggaaattgtc atccaaagat
     5161 tagtaaaaaa tggatggtca gacaagagaa tatccaaaga attaggcatg gacattgaag
     5221 aaatttttcg ctttaagcaa gtgtctggtg gaatggcaac agtttttttg acaaatttta
     5281 taaggtgcag aacttctttc cgtatgctat tccgattgtc cttgacaatg agcctcctcg
     5341 gatgtgacga tcttcggcag gagctaacag ttaaagttag actgcctcgc tagcgttagc
     5401 acatccgagc tcattatcaa ggattcgctg cgccaatctc tggttacggt aaccaaccgg
     5461 tgtctcgtag ccaagtgtac cgtgcaaccg aaggtggttc caccaattta catagtcaaa
     5521 taactccaaa tccaattgtt gtaaggtttc aaatgtgtat tgatagacaa attctacttt
     5581 caacgactta taagttgatt cagctacggc attatcaaaa ggacagcctt tatgactcaa
     5641 tgatcgattg atgtcaaaag ttgttaataa ttcatcaata gcttggttat caaactcttt
     5701 tccacgatca gtatgaaaaa tctcaacctc tgtcagaggt tgtttgatac ggctaaatg
//
LOCUS       DAJSBN010000103         5449 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJSBN010000103 DAJSBN010000000
VERSION     DAJSBN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.106"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(152..550)
                     /locus_tag="ONT86_002691"
     CDS             complement(152..550)
                     /locus_tag="ONT86_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR2353430.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ONT86_002692"
     CDS             complement(547..1320)
                     /locus_tag="ONT86_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR2353431.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ONT86_002693"
     CDS             complement(1317..2000)
                     /locus_tag="ONT86_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR2353432.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ONT86_002694"
     CDS             complement(2167..3054)
                     /locus_tag="ONT86_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2353433.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ONT86_002695"
     CDS             complement(3051..3920)
                     /locus_tag="ONT86_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2353434.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ONT86_002696"
     CDS             complement(4057..5343)
                     /locus_tag="ONT86_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2353435.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         1675 a         1216 c          905 g         1653 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctc
//
LOCUS       DAJSBN010000104         5359 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJSBN010000104 DAJSBN010000000
VERSION     DAJSBN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.107"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            25..696
                     /locus_tag="ONT86_002697"
     CDS             25..696
                     /locus_tag="ONT86_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2353436.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ONT86_002698"
     CDS             755..1195
                     /locus_tag="ONT86_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353437.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ONT86_002699"
     CDS             1207..1875
                     /locus_tag="ONT86_002699"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR2353438.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ONT86_002700"
     CDS             1939..2562
                     /locus_tag="ONT86_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCR2353439.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ONT86_002701"
     CDS             2618..3004
                     /locus_tag="ONT86_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCR2353440.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ONT86_002702"
     CDS             3217..4608
                     /locus_tag="ONT86_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCR2353441.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1778 a          883 c         1124 g         1574 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagtc taatggtgat
     5221 aagtcaagat gaaatttaag attttcttta ttgagtaata tgttttgaag cgcacgaaac
     5281 tagacccgat caaaatcaaa tttttttcgg gttgattcaa ggatttgcgt caataacgga
     5341 ttgttatttc tctatcaca
//
LOCUS       DAJSBN010000105         5250 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJSBN010000105 DAJSBN010000000
VERSION     DAJSBN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.108"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1666)
                     /locus_tag="ONT86_002703"
     CDS             complement(<1..1666)
                     /locus_tag="ONT86_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCR2353442.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQN"
     gene            complement(1708..2766)
                     /locus_tag="ONT86_002704"
     CDS             complement(1708..2766)
                     /locus_tag="ONT86_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HCR2353443.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(2885..3340)
                     /locus_tag="ONT86_002705"
     CDS             complement(2885..3340)
                     /locus_tag="ONT86_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353444.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(3924..4727)
                     /locus_tag="ONT86_002706"
     CDS             complement(3924..4727)
                     /locus_tag="ONT86_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2353445.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1551 a         1026 c          727 g         1946 t
ORIGIN      
        1 aattttgacc atatactctt tctttcctag gtggaagacg taaatctata tttaaatgtt
       61 ttcgtactaa ttcagcactc ttcaacattt cattataagc aatttccaga agagtttgtt
      121 cggataaagc ccaaaatgga actattcgtc caaatcggtt aataacttca ggctttagac
      181 cttcaccaat caaggcttgt ttgatccctg atgcgacttt tcggtattct ctttttagtt
      241 cagtgctgta ttcattagaa ttttccttgt ttgtaacacg agatagtgag ctatatcggt
      301 ttaacgaatc gaacatctcc gcacctgcat ttgttgtcgc aaataaaatt gtatttgaca
      361 atgaaacggt gtcgtgaact tctcgatcat cccatcctct ttcagtaaaa cttatcctac
      421 cttcatctaa aattgataaa aaggcatcga gtgtttctac accagctttc tcatattcat
      481 cacacacaat aatcgcagaa ggatcatgcc ttacatgagt acctataagt cgtctaaaac
      541 gcattgcatc atctttggtt ttgtagtccg gcatattaac taagagaact ttattttcat
      601 cgccataaag tcctcttgcc acggctttag ccattgcagt tttaccgacc cctgttgatc
      661 ctgtaaacag agcagttgct tttggttgct ttcgaccttt aaaacgatca aatccaagct
      721 tcatacgttt aaacaacatt gttatagtat aaactgctaa cggttgtcct tttatggact
      781 ttaattcctc ttgaagtttt tccatatcaa aatcagaatc tagctgaata ttatacttac
      841 caaatatttg caatagactt tctcgattat aagcatttcc gtctactcga tcttttgcaa
      901 tcaaatcctc cattacagca attgatttag ccggttcgga caaatcctca cgataaagtt
      961 tgtttgcttg tacgatttct tgaagtaatt catcaggaat cctttgcgat aatctaaaag
     1021 aagcctgagg atcatcttta acagaggatg tatttagcca ttgtctcaaa atatcgacga
     1081 caacatcgct ttttagttca ttcaattcta acggttgtag ccgtctccct aaggcatcat
     1141 ctcttttgat atagttttca tattcttttc ttgtagtagc ggtaatcatt tgaagtgatt
     1201 catttgctaa cggttctttt agcgaatcac ccccaatttt attgccactc ccaaaagagc
     1261 taacaacttt atgaaactcg tcaatgaaga ggacaaattc attatctttg tcctcagctt
     1321 ttaaaattcg ctcatattct ttcaatcttg gaattaatgt ttctaaacgt gtttggagga
     1381 catcatctcc cccaacacgc aattgtccga ttgataattt atatacttgt actttctttc
     1441 catcttgtcg ctgtctttca gcccattcac ttgcaagtgc tgttttacct gttccggctt
     1501 cacctaacaa gatagcttgc ggtgtttccg gtctattcaa gatcagtgac aactcatgga
     1561 gttcgtcatc acgtcccact aacttatgtt ttggtggttt cattggtgtg agagcatgat
     1621 taaattcggt ttgaatattc ttaaaaaact cggtactatt ttccatctgt ttgtgttcac
     1681 ttcctctaaa ataagaataa tttacttcta gtcagtcaaa gttaggtact taaatgtcgt
     1741 cttcatgtct ccaaaagaat ctttgttaaa gtcagcattt ttgtattgat caatcaattt
     1801 ttgtgtatca ttgattgctt taatttcggt ctccattcga ctgtctagtt gacgaatact
     1861 ttcttgcgaa tctttatcgt caggattttg ttgtaaacgt tccagatact cagcttttgt
     1921 ggcttcaatt ttcttgatgt tagtatttga agcatttaac tgatcttcta gcttatttac
     1981 aacattattc ttacttgtaa ctgctacaac ttttgaataa tcaatatcgc catctttggt
     2041 aagaaagctt ccttgataga cattagttga atctgaatat ggatttattc tgaaattgat
     2101 attatcagga gtattaacag gttttgcttt tttcccaaaa gaaattactc cttcattatt
     2161 aacatcattc atagaatatt gagaaagtgc ttttgtaact gaatctgctt cattcacatt
     2221 gactttggta tcatcgtcag ctgtatcatc accagaaatt agtgatactt ggtttgcaat
     2281 aaaaacttga taagctcttt cctctaattt accttttagg aataagaaag catccccatc
     2341 agtacttaac atcccatagc tcatttcaag cttcttagga cgatcttttt cttttgttgc
     2401 catatataag ttatagtttt ttccttgcgt agataacaac tttctagcgt tttgggagta
     2461 accaagtttt actactgtta catctctatt ttgatctgtc cacacatctg aaatagtgat
     2521 ttctgctcct gatcgagcaa actccaatga agaccctata ggtgttgtat tgttcacgag
     2581 ataataatta tagtttccta attgataaat gtagctaccc aagcctgtta ataaaaaaag
     2641 cactcctaga aaagttgcaa gcataatgcg taatccgtga cgtgttctct tatccttatc
     2701 accaagcaac cctccaataa tactgtattg atctttggta gatgttgaat tgacgttttt
     2761 ttccatcagt ttgtctgccc cctttcataa aagagcaaac aatcagacgt tttttcggta
     2821 attctaggtt cgggattttg aatcatttcg gtatattctt ccccattaat atttaacaca
     2881 aaaattatct tcctttcttc gttcctttct tttgctcttt attttccaaa taatcaatga
     2941 tttttgctat tgtaccaaga tagtaattcc cctcagcttt gttttttcgg attttgctaa
     3001 ccattggagt aggtacaatc tcttcatctc tcaattcact gaaagatttt ttacctagat
     3061 attttccaat aacagatttt aaaaaacgtt caaaatcttt ctcgtaagtt gttttcttgc
     3121 cttttagttc atggttcata ttgtcaacga cttttgcggt ttcaataatt gttttagttc
     3181 tcagcttaat aatttcatct ggaattttca atcctcgtaa tttcatcgtt gtactaactg
     3241 gtaacccatg ctttactgca tcagcaatac taaaactttt tagatcatct agtaagctct
     3301 ccatatagtt aacaaattca tgccaattaa tagcattcat tgtctcaccc cttcattttt
     3361 ttaaaatgag gttcgggcgt aacacattta tgttattata atatcataat aacataaata
     3421 aatcaatcag caaatttaga tatcccttaa agtatcagca attattcaac aagatattta
     3481 ttttagctat ttcagtcatc ataaaagact aattagcaat aaaataacta atattatcca
     3541 tttatcagac gaaaagaaaa aacttatcta attactctat attaactttt aacaaatagt
     3601 tgacttgtcc aaaaatgtta ccttcaatct cacaaagata ttaagatgtt tcctaccaaa
     3661 actgatatca ccggactttt tattctgtgt tgtccaaaag tgttaccttt agtcgatcaa
     3721 caaattaaaa aaaattgttt ttttcttgac ttgtccaaaa gtgttaccca tagttgttaa
     3781 aatttgtata gattagccat tctttcgaat attatttaga ctgaaacttg cttacaaaaa
     3841 aacttcttgc atttattgtc caaaagtgtt acccatactt ttttaaagaa gcaggttgta
     3901 ttaaaattac aacctgcttc ttctcaatct tcttctaacc aattatgcat tgaaatggtt
     3961 ggcaagtttg attctggctc actaaaagtg aatctgagcc gatcaactct tcttcctttc
     4021 tttgcataga ttttttcata atcaaaagtt tcaaatacct gtattccgtt ttcatcagga
     4081 gctaaaaatt cttctttcgc ctgctctagt attcttttgt caatatttgt cattctataa
     4141 cttgaaggta tacttaatgc tcttctaaaa tcttcaactt tgactaccca ataaccattt
     4201 cgccactctt tatttctgta ttgcatgaga tgacgataca attcttttgt gtagctactt
     4261 cttagttttg taaaattatc aagttcaaaa cgagtaaagt ttttgccaat tgagtttaag
     4321 ataaattcaa attcctcatt tgccttaatt gtaactgttt ccttctgttt atctatccgg
     4381 aatttttgaa aaagccacag ctgttcatag acgtctgaat cttcaaaacg gtaggttaaa
     4441 gtacctagtt tgtcactcaa agcttttaac acagaataaa aactgtcctt ttctcttctg
     4501 tcgtatttga ccaaataacg aaagaggttg aaatcaaaag ttacttctgc tgttccttga
     4561 cgtttcattt ttgataaaat agcccaaaac acatccattt caacaggctt aaatcctcgc
     4621 aaagctgttg tattcagacc attgttataa cgcacaattg catttgtttg ttctgatact
     4681 aaatctacat cactatcaat aatcttttcg gtaacttctt gagacattga ttactctcca
     4741 atcttctttt tgcgctcctt taatcatgtt aatctggtac taattaaagg aaacaatttt
     4801 ttctgcttgt tacctaaatt aaatcacaac taaaggtaac agtcaacaaa gatttgttac
     4861 taatctaatt ttctattttt tcgccttcca aatcaagaaa accgaaggta acgtcttatc
     4921 ctagtgtgat acctcaaatg tccacttttg ttacctttgc tgtccaaaag tgttaccctt
     4981 actgtccact tttgttacct ttactgtcca aaagtgttac ctttataccc ctttaatact
     5041 tactctccca agagattaag cctcccctaa cagatatatt aacagatatt taacagatat
     5101 attaacagat atttaataaa ggaggattac tcctctctta tttcaaaatc ttaaaaataa
     5161 aaaatcttaa aaaaaactaa cactatctag gagtttattt tgcttgtgta actttgaata
     5221 attgaaaaaa aggaaaagga ggaagcttca
//
LOCUS       DAJSBN010000106         5212 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJSBN010000106 DAJSBN010000000
VERSION     DAJSBN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.109"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            604..954
                     /locus_tag="ONT86_002707"
                     /pseudo
     CDS             604..954
                     /locus_tag="ONT86_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
     gene            948..1370
                     /locus_tag="ONT86_002708"
     CDS             948..1370
                     /locus_tag="ONT86_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353446.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2540..3508
                     /locus_tag="ONT86_002709"
     CDS             2540..3508
                     /locus_tag="ONT86_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2353447.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3525..4049
                     /locus_tag="ONT86_002710"
     CDS             3525..4049
                     /locus_tag="ONT86_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR2353448.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTETHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            4425..4706
                     /locus_tag="ONT86_002711"
     CDS             4425..4706
                     /locus_tag="ONT86_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353449.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4708..5124
                     /locus_tag="ONT86_002712"
     CDS             4708..5124
                     /locus_tag="ONT86_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353450.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1967 a          893 c          868 g         1484 t
ORIGIN      
        1 aaaaaaaaga aaaatccgct tatgcaactt cttctttttg tattttgggg cgctacccca
       61 atccccaagt atcggttatt taatagcgcg gaaatccaaa gaataggacg taccctgaaa
      121 caaaaaaaga ctgctctcac gagcagccca aaatgagatt atcctattca tcaacataaa
      181 aatattacaa agtgaagaac tagagaaaga caaaaaaagc atatcaaatc ctctcaaaaa
      241 taacaagtct taaaaaaatg acagataaaa atgcacaaag aattagatat agataaccgc
      301 gaaaaatcaa gctttagaaa gttgctttta gctagaccaa ttaaaaatca caaagtgagc
      361 gtctctatcc attggtagag gggctacttg gcatagtgta cattatgtta actagctcga
      421 taaataacga ttatgttccc aaaacaatat tttatgaact ataacaaaaa tttgttatcg
      481 catattgtat attttaatta tataattata ggtgtatacc acttaaattt aacacaaagg
      541 cgtggttatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
      601 tggaatagtc aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat
      661 tactgtggtg tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca
      721 caactaagaa aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac
      781 agccgtcatg cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat
      841 gtgtggaatc cttggtataa ctacccaact gtaataagtg caaatgcagg accagttaca
      901 attcctgtac ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac
      961 ccattgcttt agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac
     1021 aagaaataca aaagactgat ctgattcctt ttctaggaac ttggcaaact aaagatcatt
     1081 cagaacgtct agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct
     1141 tagtaaaaga gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac
     1201 aagttttcat ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat
     1261 tagattacac ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg
     1321 atgggacaga agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg
     1381 tttgatatta caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt
     1441 ggctagcatt tgctagcctt ttttgtattg tggagcgtgg tttttgaggg ggattcaagg
     1501 gggagcttgc gcccctttga caagcggtta tttgcaccaa atacgtaggt atttggctag
     1561 caaatataga gcttgccaaa cactttagtg agttttgtag aaagtacctt taaaggtact
     1621 ttcacattac gaacgatacg tccgtaggac gtactgaaag ggtgggattt atgagcgaac
     1681 taatgcaaaa tttggctagc aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat
     1741 gaacactttt tcaagtgagc gaatccgcca tgacaaagct gaagcagttt atttcagctt
     1801 tgtcatggcg tgtgcctaga cttttaaaaa agtcggcaca cgagcaaata tctttatcaa
     1861 gctctaattt gcgttttaag acgtttgaaa cgtctaagta tataaatata taagagtagg
     1921 tttaagagtg cttaaatcga attttagagg gcattcagac aactttaggg ttgttagggc
     1981 ttgccctgac cgcctgtaag gcgctttaag aacaagataa gggatttata ctaagactgt
     2041 cttaaaacgt ctcaaacgaa ggctcatagc ttctcaaaga gttccttatg aacattttta
     2101 gcaaaggagc cggaagcgac tgcgcagtat aatgtgagag cagagcgatc acaccttttt
     2161 aagcgactgt agggagtaag attcccagtt gagaaaaatg cgcgaatctg tttggagaaa
     2221 aaagaagtgg ttttttaggc gggaaatttt gtccccctct tttataatct ctttttaaac
     2281 ctctttttaa acctctttac cgtcggctat atccttactc tcccaaggga atgacgcttt
     2341 tatctacatc gttttgtctg tttatctaca tcgttttgtc tgtttatcta catcgttttg
     2401 tctgtttatc tacatcgttt tgtctgtttg tacattgaat tagtacatcg acgatgttaa
     2461 tataagatgt agataaacag gcattttgta tttcataacg tgtatctctt gtgcgagtaa
     2521 gaaaggagct attttaaata tgtctaatga aatcgtaaag tatcatcatg aattaaatac
     2581 aattccgttg cgaaaattta caccagtcga aatgaatttg ttcttttcta tcgtatcaag
     2641 aatgcgtgat gtaggtgata aaaaagtaca atttacattc gagcaattaa aagatttaag
     2701 caattataaa gcaactgcaa atgttcgatt catagatgat ttagaaacaa cgtatgacaa
     2761 attaatggat ttacgttttg gtagaagaag tgctgacggt ttgcaacgtg aacgttttgt
     2821 tttatttaat caatttaaaa tcgacggaaa atctgatatt ccttttgctg aaatacaagt
     2881 tcatgaaaaa gccttaccct tacttaataa tcttgaagaa tgggtgcgct attctctcca
     2941 acaattcaat gaattagaaa gcagttattc aaaaacaatg tttcgattac taaaacaata
     3001 cagaaccaaa ggtttcgctt atttttcaaa agaagacttt catgaattac ttgatattcc
     3061 taaaagttac aaccaaggta cgattgatca acgtgtgttc aatccaatac gacaagaact
     3121 aaccgcaatc tttaaaggtt taacaatcaa aaagaaatac ggaaaaggac ggggaaagcc
     3181 tgtcattggc tatcaattta catttaaacc tgagataaaa aaatcagacg actttaacaa
     3241 acatgcccca acaaaagaag aacaaaacaa agcattagaa ctagctaacc aacaagttca
     3301 atctgaaaaa gcacctactt cattacaaga aaaaatcaaa gaacaaaagc gaaaattgga
     3361 acttttacaa cagttagaaa agttagaaag agaacaaaat acccaaaata aagaaaataa
     3421 tgcacaaaaa tcagagataa attccgatat tccttcagaa ctaaaaaaag acttattaga
     3481 caatcttcaa aacttattca aagattgagg ggtttttaga ttttatgaca gaaaaaacaa
     3541 tcaaagaact tgctgaagaa ttaggagtta gtaaaacagc aattaacaaa aaagtttctg
     3601 acgaaaatcg aaaactttgg ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg
     3661 gacaaaaggc tataaaatca atgtttttta acgaaaacga aaactcaaat caacaactag
     3721 ttggcgaaaa aacgaaaacc gaaacccaca aaaacgccaa ccaagttccc gaattagaag
     3781 tcctttctct cctagaacaa cagttaaatc aaaaagatat gcaactacaa gaaaaagacg
     3841 agcaaataaa acaaatgcaa aaattactcg accaccaaca acaactcacc ctgcaagcca
     3901 accaacaaat cgagaaactg caggaacaac tacagttgac ttatacggaa gaatcttcgg
     3961 aaaataaatc aacgactctt tctgaagaaa cagaatcgat agaaaaacaa tcaaagacgg
     4021 agaaaaaatg gtggcaattc tggcaataaa accagcaatt taaattaata aaaattgctg
     4081 gttttattct tctttttgct tttgcgagca gagcaacatg gacccatgtt gcgatatttt
     4141 ggtggcgccc caatccccgt tttttcgttg aatcttactc ggactgcact cgttttacag
     4201 ctgatttttc tgaattttga aagagcataa ggaaacgtgg tatcactttc ttacgaaagg
     4261 tgctagatag gctgtgccta tcacgtttct cagaaaaaaa tttgctatca cttttttgcc
     4321 attgtggcta ggctatgtat agtgttttgg cgaaaaatag ctatacaaat actatatttg
     4381 tatggtatac ataaattata cgctgatacg aaaggaaaat ttccatggac attttagttc
     4441 gaaatatcaa tcccaaaatt attgcaagaa tagataaacg ttgtcaggaa ctcacaacca
     4501 aaacgggcaa aaaatggagt cgaaataaat atctaaaagt tctaatcgaa agtgattttg
     4561 accgtcaact tatgaattac aaaaaagatc aattcgattt attactcgaa aaatttattg
     4621 ccattcaaac agaaaatacg aacatactga atgactatgt tcacacaaac aaccaactca
     4681 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgtaacc
     4741 ctttatccca gtcacgttga cgttttagat tccatcatcg aaaaaaatcc agacgtcaac
     4801 aattattcag aagccattcg ttttttatgt atgagttatg aacctgatac caatacaacc
     4861 acaaccagta accaagcaaa actcaatgcc atgggaaaag aaatttctat catgacagaa
     4921 atcatggcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4981 tctaaagtgt atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ttcccgacga
     5041 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacct ttacttctgg aaattttgac
     5101 ccaacaaact ttctaaaaag ataaaaaaag aaaccgatcc ctaaaaaaat tcctcgattt
     5161 ctttttaggg atcagtcttc cagcaagatt tgcaagttgg taccaacttg cg
//
LOCUS       DAJSBN010000107         5015 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJSBN010000107 DAJSBN010000000
VERSION     DAJSBN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.111"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            286..1236
                     /locus_tag="ONT86_002713"
     CDS             286..1236
                     /locus_tag="ONT86_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCR2353451.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            1257..1790
                     /locus_tag="ONT86_002714"
     CDS             1257..1790
                     /locus_tag="ONT86_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR2353452.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            1806..1976
                     /locus_tag="ONT86_002715"
     CDS             1806..1976
                     /locus_tag="ONT86_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353453.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            2426..2959
                     /locus_tag="ONT86_002716"
     CDS             2426..2959
                     /locus_tag="ONT86_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353454.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            2971..3174
                     /locus_tag="ONT86_002717"
     CDS             2971..3174
                     /locus_tag="ONT86_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353455.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            3496..3876
                     /locus_tag="ONT86_002718"
     CDS             3496..3876
                     /locus_tag="ONT86_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR2353456.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            3858..4772
                     /locus_tag="ONT86_002719"
     CDS             3858..4772
                     /locus_tag="ONT86_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR2353457.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
BASE COUNT         1874 a          880 c          917 g         1344 t
ORIGIN      
        1 tttgtcaccc tcttttataa tctcttttta aacctttttt taaacctctt taccgtcggc
       61 tgtgtcctta ctccttcaat ggatacagcg ttttataaac tacattttgt ctgcttttaa
      121 actacatttt gtctgctttt aaactacatt ttgtctgctt ttaaactaca ttttgtctgc
      181 ttttaaactc tttttttgca ctcacgtatg ttataatatg cattatcaca gacaaaacgt
      241 tgtttataac gtctgtatcc attgaaggag taaggaggaa tatctttggc aaatgaaatc
      301 gtgaaatacc atcatgaatt aaataccatt ccgttgcgga aatttacacc agttgaaatg
      361 aatctatttt tctctattgt gtctagaatg cgcgaaaaag gcgataaaac agtccgtctt
      421 acttttgacc aactaaaaga tttaagtaat tataaagcaa ctgctaataa tcgtttcatt
      481 aatgatttag aaatgactta tgataaatta atggatttac gttttggtaa aagaagtgct
      541 aatgggctcg atatagatag attcgttatg tttagtgagt ttaaaatacg tggaactgct
      601 aaagatccat atgtagacat acaagtttac gaaaaagctt tacctttact aaacaattta
      661 gatgagtggg ttcgctattc actccaacaa tttaacgaat tacaaagtag ctatgcaaag
      721 actatgtttc gattactaaa acaatatcga actaaaggtt ttgcttattt ttctaaagag
      781 gattttcatg aactattaga tattccaaaa agttactgga acaaccaagc aaacgttgat
      841 aatcgtgtta taaagcctat acgacaggaa ttaaccgcaa tcttcaaagg attatcaatc
      901 aaaaagaaat acggaaaagg gcgaggaaaa cctgtcgttg gctatcaatt tacgtttaaa
      961 ccagaaatca aaaaagcaga tgattttaat aaacatgcac caaccaaaga agaacaaaat
     1021 aaagcattgg ctttagctga taaacaaaaa gaatacgaag aacctgctac aacaattaac
     1081 gaaaaaatca aacagaagaa acgagaactt gaactcctcc aacaaatcaa acaactagaa
     1141 aaagagaaag aagctcttga aaaacaaaca gagtccgcac tttcgaaaaa agagaaaaaa
     1201 agtttattat ctgatttaga taatttattc cagtagcaac ctaggaggca accagtatgc
     1261 aaccacaaga aacctttaca ataaaggaat tgtcagaact ttttaagatg agtaggcaag
     1321 caatatcgaa acatatccaa aaattagatt cttccatgat agctaaaaat gagcgtggtt
     1381 ataaggttgt attgcgttct ggagtattgc aacttgctcg caacttaggc aaccaaatgt
     1441 tgctagatag gttgcacgag tacgaacaag acaacgataa aaatgttaat ataccaagca
     1501 aaaacgtggt tacaaacaag ttgctagatc agttgcgtga agaaaatgaa tttttacgag
     1561 aacaacttgc cctcagagag caaaataatt ctcatataca aaaactactc gaccagcaac
     1621 aacaactcac cctacaagcc aacaaacaaa tcgaaaaact gcaagaaaaa ctacagttga
     1681 cttatacaga agaagctcca gagaatgact caacgactct ttctgaagca acagaaacaa
     1741 tagacaaaca aattgatact gaaaaaaaat ggtggcaact ttggaaataa ctggagggaa
     1801 cattcatgac aagaaaaaat cttctattcg gttctatttt actaggaatt ctaggattca
     1861 gtccgatgtt attgtcagtg atcaataaag ggaataaaca tggtgaattg aaattgaatc
     1921 caaattatta caacagtcct aacgaagaat tgtttgtaaa agaaacagat aaataataaa
     1981 aaagactgct ctttcgagca gccagttaaa aaataatgag tgtttattct tgaaaaagca
     2041 acgcgcttat gaagcgctac caaaaattta tcatatttta ttaaaaataa caacccagta
     2101 aaaaatgact ggtaaaaatt cacaaagaaa cggaaagaag caaccgcaga aaatcaagct
     2161 taaaaagagc ttttgaccta gaccaatcga aaatcacaaa gtgagcgcct gtatctattg
     2221 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
     2281 tcctaatgca ttctaactat accacattat caaaaatata aattatcttt actaaataaa
     2341 atattgacaa gcctatacct tttgttttat gattttcaca aagaaaacgt tttctatata
     2401 tttttctgta tcaggaggaa attctatgaa aaaatttact ttaggggtag ctttgttaac
     2461 tattggggca gttgttttac cgtcaactac tatagtttat gcttcagaaa attcccaagt
     2521 ggtatcatta aacgaaaaca atgctactga aaatgtagtt attgagtaca ctgaagatgg
     2581 tatggtcgta actaaatatg aggatggtta tagtgcggaa gattttgcaa attcttcaga
     2641 tatcatgaca agagctgcag ttgcaagatg gggcaaaaag gaatatacaa atattgctgt
     2701 aaccactgga gtagctgcta atgctattaa tgctgccttt tgggcaggag ggaaagctag
     2761 cacttctatg tttggtatcc caggttgggc aattggtgct ttgttaacag gtgcaagttg
     2821 gactaaaaaa ggtgcttctc cagggaatgc agttgcaaaa gaatgggaca aaaatggtaa
     2881 tggctggatt ggattttatt accaaagggg atatgatgca gctggaagag ttgtagctac
     2941 tagatattca acgttataag ttaaggagtg atgtttttga tatctaaatc attaaaatca
     3001 ttaaaaacat ttactaaaaa gaatccgcaa atagctacta ttcgtatttt tatatttgct
     3061 atagctgttg ctataattgt taatatgcta agcggagggc aattatacaa atctggttta
     3121 tttttagtgt taatcttagt tgtcttagac ataataaagt ctaaaaaaaa ataaatcgat
     3181 acattttaga agaatagggt tgtttgaata ttacaaagca acaatttcac cttcggtgaa
     3241 ataagcgaaa ctttacaaaa agtggctagc aaatgctagc ctttttttgt attgtggagc
     3301 gtggattttg agggggattc aagggggagc ttgcgctcct ttgacaagcg gttatttgca
     3361 ccaaatacgg aggtatttgg ctagcaaata tagagcttgc caaacccttt tagtgagctt
     3421 tactcattgt acctttttag gtacaatggt attgcgaacg atacgtccgt aggacgtact
     3481 gaaagggtgg gacttatgag cgaacaacat caaaatttgg ctagcgatca ttctaaaaaa
     3541 tatacctatc gatccgagcc aaaacaaatc agttttcgag tgagcgaatc cgaatttgca
     3601 aagctgaagc agtcagctga agctttgcaa atgagtgtgc cggctttcgt gaaagccaag
     3661 gcacaaaacg cgcgtctggt gacaccgaaa gtggcgccag acattgccca ggcaatcgcg
     3721 cgcgatttag ccaaagctgg agggaatatc aatcaaattg ccaagtggtg caatacacac
     3781 caacacgacg ttgctcctga tgatgctcaa cgtttatccg aaaacctaaa aatcatgcaa
     3841 aaggagctac aaaagatatg gcaacagtta aagtaagtcg aaccacttca tgtagccgag
     3901 caatcaatta cgcagaacca cgagcaaccg tcaaaacagg aataaactgt gacattgatt
     3961 atgccaaaag tgaaatgaaa caaatacgca cgctttatgg aaaagacgat cgtgtacaag
     4021 cacacttatt gattcagtcc tttcgaccag gagaaattac cgctgaaaaa gccaatcaac
     4081 taggggcaga gtatgcgaaa aaaatagccc ctaaccacca aatcgcgatt tacacacata
     4141 cagacaaaga ccatattcat aatcacattg tcattaactc cgttaatctt gaaactggga
     4201 aaaaatttca agcccatggg caagcatttt taaacaaatg ttatgaccta aatgacgaga
     4261 tttgtcttga acatgactta acaatcacgg aacgaggaaa gaaacccgaa aaacgaacca
     4321 tgtctgaaat taaattaaaa gaaaaaaatg aaccggtttg gaaagacgaa attcggtttg
     4381 cgattgatca aacaatgaaa aacaaaaaaa ctcgaacgta tacccaattt tgtgattcac
     4441 ttaaaacatt cggcattcat tgcttgaatc gcggaaaaaa ttttacttac gaattggtcc
     4501 acaaaaagaa aaaagtacga tccaacaaac taggaaagga ttacgaaaag gagacgattt
     4561 tacatgagct ggacagacga gaacgaacct acaatcaaca acgaaccaac gagctacgaa
     4621 gagctcgagc tagcattcgc gaaactgcag aagaagtacg aagagaacaa cgaactttta
     4681 cgcctgaaca atcggaaatt agaccgactc gaagaaacca tgaacaacac gttgaacgag
     4741 gaacagacca ccaacaagaa ttatcgctct aaaacgttaa aagacatcga aaacgaaata
     4801 aagagacaac gtgcctttct tgcgccttat acccaagaaa ggatacaaac tacgatggat
     4861 tcacaagtca aaaaactcca ccaagcgcta ttagaccagc gaaaccaaga acaaaaaaag
     4921 gctttaattc aacgatcgat ggatattctt gcccccttat gttgcttagt actaacatta
     4981 ttagcctttt ggttggtatg gggagaataa acaga
//
LOCUS       DAJSBN010000108         4886 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJSBN010000108 DAJSBN010000000
VERSION     DAJSBN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.112"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(6..284)
                     /locus_tag="ONT86_002720"
     CDS             complement(6..284)
                     /locus_tag="ONT86_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353458.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(244..1158)
                     /locus_tag="ONT86_002721"
     CDS             complement(244..1158)
                     /locus_tag="ONT86_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR2353459.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(1140..1520)
                     /locus_tag="ONT86_002722"
     CDS             complement(1140..1520)
                     /locus_tag="ONT86_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR2353460.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(1970..2257)
                     /locus_tag="ONT86_002723"
     CDS             complement(1970..2257)
                     /locus_tag="ONT86_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2353461.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(2277..2501)
                     /locus_tag="ONT86_002724"
     CDS             complement(2277..2501)
                     /locus_tag="ONT86_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HCR2353462.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(2956..3126)
                     /locus_tag="ONT86_002725"
     CDS             complement(2956..3126)
                     /locus_tag="ONT86_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353463.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(3141..3674)
                     /locus_tag="ONT86_002726"
     CDS             complement(3141..3674)
                     /locus_tag="ONT86_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR2353464.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(3695..4639)
                     /locus_tag="ONT86_002727"
     CDS             complement(3695..4639)
                     /locus_tag="ONT86_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCR2353465.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
BASE COUNT         1270 a          895 c          831 g         1890 t
ORIGIN      
        1 tctatttatt ctccccatac cagccaaaag gctaacagtg tcatgattaa gcaacataag
       61 ggggcaagaa tatccattga tcgctgcata attacttttt tctgttcttt atttcgctgg
      121 tttaatagcg catggtggaa ttttttgact tgtgaatcaa ttgtagtttg tattctttct
      181 ttggtataag gtgcaagaaa agcacgttgt ttctttattt cattttctat gtcttgtaac
      241 gttttagaac gataattctt gttggtagtc tgctcctctt tcaacgtgtt ggtcatggtt
      301 tcttcgagtc ggtctaattt ccggttgttc agtcgtaaga gttcgttgtt ctcttcgtac
      361 ttcttttgca gtttcgcgaa tgctagctcg agctcttcgt agctcgttgg ttcgttgttg
      421 attgtaggct cgttctcgtc tgtccagctc atgtaaaatc gtctcctttt cgtaatcctt
      481 tcctagtttg ttggatcgta cttttttctt tttgtggatc aattcgtaag taaaattttt
      541 cccacgattc aagcaatgaa tgccgaatgt tttaagagaa tcacaaaatt ggttatacgt
      601 tcgagttttt ttgtttttca ttgtttgatc aatcgcaaac cgaatttcgt ctttccaaac
      661 aggttcattt ttttctttta atttgatttc agacatggtt cgtttttcgg gtttcttccc
      721 tcgttcagtg attgttaagt catgttcaag acaaatctcg tcatttaggt cataacattt
      781 gtttaaaaat gcttgaccat gggcttgaaa tttgttccca gtttccagat tgacggagtt
      841 aatgacaatg tggttatgaa tatggtcttt gtctgtatgt gtgtaaatcg cgatttggtg
      901 gtcaggggct atttttttcg catactctgc ccctagttga ttggcttttt cagcggtaat
      961 ttctcctggt cgaaaagact ggatcaataa gtgtgcttgt acacgatcgt cttttccata
     1021 cagcatgcga atttgtttca tttcgctttt ggcataatca atgtcacagt ttatccctgt
     1081 tttgacggtt gctcgtggtt cggcgtaatt gatcgctcgg ctacaagaag tggttcggct
     1141 tactttaact gttgccatat cttttgtagc tccttttgca tgatttttag gttttcggat
     1201 aagcgttggg cgtcgccagg atcaacgtcg tgttggtgtg tgttgcacca cttggcaatt
     1261 tgattgatat tcccgccagc tttggctaaa tcgcgcgcga ttgcctgagc aatgtctggc
     1321 gccactttcg gtgtcaccag acgcgcgttt tgtgccttgg ctttcacgaa agccggcaca
     1381 ctcatttgca aagcttcagc tgactgcttc agctttgcaa attcggattc gctcactcga
     1441 aaactgattt gttttggctc ggatcgatag gtatattttt tagaatgatt gctagccaaa
     1501 tattgatttt gttcgctcat aagtcccacc ctttcagtac gtcctacgga cgtatcgttc
     1561 gcaataccat tgtacctaaa aaggtacaat aagtaaagct cactaaagct cactaaaggg
     1621 cttggcaagc tctatatttg ctagccaaat acctacgtat ttggtgcaaa taaccgcttg
     1681 tcaaaggggc gcaagctccc ccttgaatcc ccctcccccc cactctccac aatacaaaaa
     1741 agactagcaa atgctagcca ctttttgtaa agtttcgctt gtttcacctt cggtgaaatt
     1801 gttgcaaatc aataattagg tagagctaca tttttttcgt tgttcaaaga ttcttatgcc
     1861 atttactatt aataaatcta taaatatgat aatttttcgg ctctataatt tatgggactg
     1921 atgaatcaga attgattctt cagtcccatt ttcattttat tccaaagaat taactccaaa
     1981 taccaataga agcccatcct ctatatactt gagattcctt actaacaatt tcttgtaatt
     2041 ctattaaaga tttaggtaat ccaaatttat gttttaaaat atatcttcca agaaaactac
     2101 ttaatttcat actaacttca ccaacacttt ttccctcact gatttcttta gaataaagaa
     2161 agagcttttc ttttaagtca ggatagtcat ctatagttgc ttcattgtat actttactaa
     2221 ttgcatttaa aagtttttct tctttagtaa aatccattat aataacctcc ctaccactac
     2281 cttctaggtg cccatggacc atgattaacc caaccattaa caataatttt tccaatttct
     2341 cctttagctt gattccaatc tacccaacat ttttctttgt tacaataaag accatttcca
     2401 tagtaagtag ctgcatcaac ttttattcct gtaccgatgc cagctagaca agttcctaat
     2461 attcctagaa taacacaatg ttttaatact ttctttttca tgattgtttt tccttttcgt
     2521 ttttttaaaa ttgcttgtta tattaaataa tcttagtaat tttattatat aacattttat
     2581 tatataacaa tgattaacgt cataaaaagt agttttgaat tcataatcgt ttaaataaaa
     2641 ttataagatc agttatttaa acagctagta gacataatgt gtatactacc aagtaacgcc
     2701 tgtaccaagg gatccaggcg ctgattttgt gaatttttat tggtctaggt taaaagctct
     2761 ttttaaggct tggttttctg cggttgtttc tttctaattc tttgtgaata ttgatcggtc
     2821 attttttaaa tacttgttat ttttgtttgt gtttgttatg ctttttacat atctcatatt
     2881 catttattat tcttttctaa ggttaataat aggataatct ctttcagaaa gactgctcgt
     2941 acgagcagtc ttttcttatt tatcaagttt ttctacaaat acttcctcat tttgactgtt
     3001 gtagtaatta ggattgagtt ttagttcccc ttttttattt cctttgttgg ccactgataa
     3061 taacattggg ctaaagctta atattcctag taggatagaa ccgaatagca agtgtttttt
     3121 tgtcataagt atttccttct tcatttccat agctgccacc attttttcca attctttgtt
     3181 tgttcgtcca ttgtttctgt tgcttcagaa agagtcgttg aactattctc tgaagactct
     3241 tccgtataag tcaactgcag ttgctcctga agtttttcga tttgttggtt ggcttgcagg
     3301 gtgagttgtt gttgctggtc gagtagtttt tgtatatgag aattattttg ctctttgagg
     3361 gcaagttgtt ctcgtaaaaa ttcattttct tcacgcaact gatctagcaa cttgtttgca
     3421 accacgtttt tgtttggtat ataagcattt ttatcgttgt tttgttcatg tttatgcaac
     3481 ttatctagca acctttgatt gtctaagttg cgagcaagtt gcaatactcc agaacgcaat
     3541 acaaccttat aaccacgctc atttttagct atcatggaag aatctaattt ttggatatgt
     3601 ttcgatattg cttgcctact catcttaaaa agatctgaca attcctttat tgtaaaggtt
     3661 tcttgtggtt gcatactggt tgcctcctag gttgctactg gaataaatta tctaaatcag
     3721 ataataaact ttttttctct ttttccgaaa gtgcggactc tgtttgtttt tcaagagctt
     3781 ttttctcttt ttctagttgt ttgatttgtt ggaggagttc aagttctcgt ttcttctgtt
     3841 tgattttttc gttaattgtt gtagcgggtt cttcgtattc tttttgttta tcagctaaag
     3901 ccaatgcttt attttggtct tctttggttg gtgcatgttt gttaaagtcg tctgcttttt
     3961 tgatctcagg tttgaacgta aattgatagc caataacagg ttttccccgt ccttttccgt
     4021 atttcttttt gattgttagt cctttgaaga ttgcagttag ttcttgacgt ataggtttta
     4081 taactcgttt atctatatca ggttgcttat aacttttagg aatatctaat aattcaagaa
     4141 agtcttcttt ggagaaatat gcgaaacctt tggttctgta ttgctttaac agacgaaaca
     4201 tagtctttgc atagccactt tgtagctcgt tgaattgttg taaagaatag cgtacccact
     4261 cgtctaaatt atttaacaaa ggcagtgctt tttcgtaaat tcgtatatct acgtaaggat
     4321 tttctacgtc cccgttgatt ttaaattctg taaacattac aaacatatcg taagaaagcc
     4381 cagactcact tctacgacca aagcgtaaag ataagatttt ttcataagtt gcttttaaat
     4441 cttctacaaa tcgtacattt gcagttgctt tgtagttact taaatctttt agttggtcaa
     4501 acgtaaaacg aactgtctta tccccttttt cacgcattct agacacgatg gaaaaaaaca
     4561 aattcatttc aaccggtgta aattttctta aaggaattgt atttaattca tggtggtatt
     4621 ttacaatctc attagccata taatcacctc aatccaatta tgaatgacat tttttaaatg
     4681 tcaagacatt catatttgac gttcaaacag acaaaatgtc cttcataaac agacaaaatg
     4741 tccttcataa acagacaaaa tgtccttcat aaacagacaa aatgtccttc ataacattct
     4801 gtatccattg gtagagtaag gacacagctt acggtaaaga ggtttaaaaa gaggtttaaa
     4861 aagagatata aaaagagggg ggctaa
//
LOCUS       DAJSBN010000109         4836 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJSBN010000109 DAJSBN010000000
VERSION     DAJSBN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.113"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(57..173)
                     /locus_tag="ONT86_002728"
     CDS             complement(57..173)
                     /locus_tag="ONT86_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR2353466.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(418..495)
                     /locus_tag="ONT86_002729"
                     /pseudo
     CDS             complement(418..495)
                     /locus_tag="ONT86_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(649..1284)
                     /locus_tag="ONT86_002730"
     CDS             complement(649..1284)
                     /locus_tag="ONT86_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2353467.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1797..2939
                     /locus_tag="ONT86_002731"
     CDS             1797..2939
                     /locus_tag="ONT86_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2353468.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYVCDNPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYEY
                     GYYGYKEHYAYGEIKVMVSDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPTLSEKYKNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDNLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            2932..3138
                     /locus_tag="ONT86_002732"
     CDS             2932..3138
                     /locus_tag="ONT86_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR2353469.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            3217..4410
                     /locus_tag="ONT86_002733"
     CDS             3217..4410
                     /locus_tag="ONT86_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2353470.1"
                     /translation="MKEKRRDSKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDRLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDVLI
                     LLKTGLRISELCGLTVADIDFQNEVVIIDHQLLKSKEQGYYIETPKTKSGTRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRGNFLFINHKGKPKVAIDYTMLFVRMVKKYNKNHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
BASE COUNT         1637 a          936 c          922 g         1341 t
ORIGIN      
        1 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
       61 aagcccaaaa gggcataaaa atcccagtga taaatactct tatcactggg atttttatgc
      121 ataaccatag gcatacaaag catacagata ttctccggat actttagaat cacatgataa
      181 aggtattctt aaactgggta caaaaaacta agccctccta aaaaaggaca tccaattatt
      241 tgttcccact atcaaattga cagtttattt aagaatacct tgccgcatat ttattaactc
      301 cttttaaata gatacttaaa taatagcacg taagagcata tttgtaaagg aatctccaat
      361 tttttatcaa agagagtacg tgattacaaa atagctgtaa taatgtacca atatttgtta
      421 ttctataatc ttccaattac tcccgttctt ttcaagtacc aaatcaaatt gagatacctg
      481 cgttgccttt gcctgattta ccacaaatca gattttatcc ctttcttcct cttgattgcc
      541 cacaacagaa aagaaaaagc cctgtcaaga gctttgccac cattggtggt gctttgcact
      601 cttgatcggg ctttttgttt gctgtatctt ctcgcaagag gaaaaaagtt actcgtcctc
      661 atcatcaaaa ttgttattcc ttaatacttc tcgtaacagt tccatatctt cttttgagtt
      721 cgggtttatc attttcacga cttgataagg tgtcttatac ttatgtccct ctatgctttc
      781 cccaaacata tccatactgt ataaatcatc atagcggtct aataattttt gaaacttcaa
      841 atgctggata aattctttga ttgggtaaag gtcggacgct tcaatgcttt tcccgttgat
      901 atagtctgta atatattccc gtaacttttc taactcatat tccagccatt cttcctcgct
      961 gtcaaagaag ttatcatagt gtccatgttt cagttctgca tttagacgtt cttcggctct
     1021 taaaaactga cagacttcct tttcagattg attgacatac ttccattctc ccgacaacaa
     1081 ttcattgggc gtaacgtcca gcacttccat tatcttttcc agcgtttcat aggcaggata
     1141 gttcaaccct ctttcaatct tggaaaggct ctgcatattg ataccgattt tgtccgcaag
     1201 ttcctgttgt ttcattcccc taaattttct tatggtctgt atattatttc ctaaatggct
     1261 tattttcatt gcataaccct ccatatctcg ttatatcttc gctattataa gtctatcatg
     1321 cttaatatga tttgtaaagg caaaaaaaga tattgacaaa taagcatata agaattacta
     1381 tgttcttgta atcgaataag cacaatagaa tgaaatataa aaacattcaa aaagaaatac
     1441 ctatctgatt aaatccgtag atatgaatat cggttgaaat ggaacttgtt agaaaaggca
     1501 aaacagaact tgctgacaaa gtatttctga cacggtggcg gaagcctctc aggcttaccg
     1561 ccatgacacc ttgtgtcaag gggaagaaat gctttgtcgg ctggcgagcc tgcgagcctt
     1621 gtctgtgccc ccgttatcta acaggggggc actatttcac aaaagacaag tcaaaaacca
     1681 tacaacccca aagctgtacg gagtaatgag gagttttaag aagatagtac acttcacact
     1741 tttttgaaag gcggtttaca ggtatttcaa cgctattgtg ggattggagg aatacaatga
     1801 acaacttaga atttgcactt gaaatgaaga acaaacgtct tgccagcggt ctttcacaag
     1861 gagaacttgc aagtcttacc catgtatcaa gatatagtat caaccgcttt gagaacggaa
     1921 aagccaacgc cagcaaggaa acacaaaata ttatcctgcg ttgtctgaat tactatgtct
     1981 gtgataaccc tttttatctg ctggtggatt atctgtctgt ccgtttccca acgactgacg
     2041 cattggaagt tatccgaaag gtgcttggca tgaaagcaga ttattttatc cattatgaat
     2101 atgggtatta tggctataag gagcattatg cctacggaga aatcaaggtt atggtttctg
     2161 atgatgaaca catgggcgta tttttggaat taaaaggggc aggctcacgc aacatggaat
     2221 acgtcttaca ggcacagaac agggattggt attcattctt aaaccgctgt cttgactgtg
     2281 gcggtgttat ccgacgtttt gacttggcaa tcaacgatat gtgcggtctg ctggatattc
     2341 ccaccttgtc tgaaaaatac aaaaatggcg gtgcggaatg tcgctgtaaa aactatgaaa
     2401 atgtgcaggg tggaaaacta agcggaaaaa accgtaattt agcaagcact ctttatatcg
     2461 gctcaaaggc aagcacaaaa tatttctgcc tgtatgaaaa acaaaaggaa caggcaacta
     2521 aaaagaaaca tacggatatt atcaaccgtt ttgaaattcg tctgcgtgac aaaaaggcag
     2581 tacaggcagt tgaagaactg ttattgacgt ataaccctca tgggctggtg ttttacctca
     2641 ttaccgattt tgtggaattt cccgactatc cattgtggga gatattcatt tcccatgata
     2701 atttaccttt tgaaatgaac cctgtacctg tcaatatgga acgcactctg caatggctgg
     2761 aaagacaggt catgccgtcc attgtgatga tagaagaaat cgacaggctg acaggttcaa
     2821 actatatgaa aatgattgat gaatgtaccc acctttccga aaaacaggaa atgcttgtgg
     2881 agcagatgtg tacggacata gcagacgtaa tcgaaagtga gggggtgttt tatgagtaat
     2941 gcacaggata ttcccgtatg ggaaaaatac acccttacca ttgaagaagc gtcaaagtat
     3001 ttccgtatcg gagaaaacaa gttaagacgc ttagcagagg aaaacaagga cgctggctgg
     3061 ctcattatga atggcaaccg catacagatt aaacgccgac agtttgaaaa ggtcattgat
     3121 aaattggacg caatctaatg caaatgagcc ttgtatgtgt tatgatgaac acaagtcata
     3181 tcaaggctct ttccaacaag gaaaggagca gacaccatga aagaaaaaag acgggatagc
     3241 aaaggacgta tcctgcatac tggagagagc caacgaacag acggaaaata cttatataaa
     3301 tatgtggacg catttggaaa cacaaaatat gtgtatgctt ggagattgac acccacagac
     3361 ccgacaccaa aaggaaaacg ggaaaaaccc tcacttcgtg aactggaaca gcagataaga
     3421 cgggatattg aggacggtat cgacagcaca ggcaagaaaa tgacgctttg ccaactttat
     3481 gccaaacaga acgcacagag ggcaaacgtg aagaaaagca cacagaaaca acgggaacag
     3541 ctcatgcgat tactaaaaga ggacaggtta ggtgctagga gcattgatac gataaaaccc
     3601 tctgacgcta aggaatgggc gttacgcatg aaagataaag gcttttccta taacaccatt
     3661 aacaaccata aacgctcgtt aaaagcgtcg ttctatatcg ccatacaaga cgattgtgta
     3721 aggaaaaacc cttttgattt caagttaagt gaagtcctag aaaatgatac caaagagaaa
     3781 gtcgcattga cagaagaaca ggaacaagcc ctactctcat tcatcaagac ggacaatgtg
     3841 tatcacaagt attatgatga tgtgctgata ctgttaaaga caggacttcg tatctcggaa
     3901 ctgtgcggac tgacagtagc cgatattgat ttccaaaatg aagttgtgat tatcgaccac
     3961 cagttattaa aaagcaagga acagggttat tacattgaaa cgcctaagac aaaaagcgga
     4021 acaaggcaag tgccattaag cagagaaacg atacaggcat ttcaacgggt tatgaagaaa
     4081 cgcccaaagg cagaaccatt tgtgatagac ggacggggca actttctatt tatcaatcat
     4141 aaaggcaagc ccaaagttgc cattgattac actatgcttt ttgtccgtat ggtaaaaaaa
     4201 tacaataaga accataagga caacccattg ccacatatca caccgcatac actacgccat
     4261 acgttctgca caaggctggc aagcaagaac atgaacccga aagatttaca gtatatcatg
     4321 ggacattcaa atatcagtat cacaatgaac tggtatgccc atgcgtccat agataccgca
     4381 aaatcagagg ttcagcgtct aatcgcataa gaagtattta ccacgatttt aaccacgatt
     4441 gacagggaaa atataagaag atagacctag atatgtgagg tttaccacaa aagcaaaatg
     4501 cccgtagagc cgataaaata aggctttacg gacatttaag aagatataaa aagatagtca
     4561 aaaagacata tataatttat acctaattta agtaagtaca aacatcattt taagaatagg
     4621 aaaggacatt tatatactat tctctgtatt tttgatttat aaataaatat gaattgaatc
     4681 caaatcagct atattactat tttataataa ccaactataa aaaacgaagt taggtgaaaa
     4741 actaacttcg atattcataa aagactctat tagttcaact tagcaaaaga tttaaacaaa
     4801 aaaagatgaa ctatttctag ttcatctttt tgcctt
//
LOCUS       DAJSBN010000110         4797 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJSBN010000110 DAJSBN010000000
VERSION     DAJSBN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.114"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            68..1114
                     /locus_tag="ONT86_002734"
     CDS             68..1114
                     /locus_tag="ONT86_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR2353471.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1131..2354
                     /locus_tag="ONT86_002735"
     CDS             1131..2354
                     /locus_tag="ONT86_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR2353472.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2443..3330
                     /locus_tag="ONT86_002736"
     CDS             2443..3330
                     /locus_tag="ONT86_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR2353473.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3416..3646
                     /locus_tag="ONT86_002737"
     CDS             3416..3646
                     /locus_tag="ONT86_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCR2353474.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3703..4668)
                     /locus_tag="ONT86_002738"
     CDS             complement(3703..4668)
                     /locus_tag="ONT86_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR2353475.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1622 a          794 c          975 g         1406 t
ORIGIN      
        1 atagggctta agtttgagtg acagtttgta ggctatgtta tactatataa gtgaaggggg
       61 acgtaaggtg aaggtattat tatattttga aagtgaaaag atgcttgcaa aatctggaat
      121 cgggcgtgcg cttgatcatc aaaaacgtgc tctgacggaa gtcggtatct cttatacact
      181 agatgaaaaa gaagattatg acatcctgca catcaataca tacggaatca atagtcataa
      241 tatggtcaat aaagccagaa gagaaggaaa aaaagttgtc tatcatgcgc attctacaga
      301 ggaagatttc cgaaattcct ttatcggatc gaaccagctt tctcctatcg taaaaaaata
      361 tctggtagga ttgtaccaaa aagcggacta tctgatcacg ccaacacctt attcaaaaca
      421 gttgttggaa agctatggta tccgtgtgcc gattcaagct atttccaatg ggatcgactt
      481 agagaaatat caccctgatc caatgaaaga acaaaagttc agagaatatt tcaaactgtc
      541 tccggatcaa aaagtaatca tttgtgtcgg tttgtttttc gaacgtaaag gaatcattga
      601 tttcgtagaa attgctaaga agatgcccga atacacattt atttggtttg gtcatgttcc
      661 catgtactcg attcctcgga acatccggaa aattgtaaaa gaagatcatc ctgaaaatgt
      721 cctgtttcct ggttacattc gtggagagat catagaaggt gcgtattcag gtgcagatct
      781 attctttttc ccttcttatg aagagacaga agggatcgtt gttttagaag ctttagcaag
      841 caaacaaaac gtacttgttc gagatattcc agtgtataat ggctggttag aagatagcaa
      901 gaactgctat atggggaaga caaacgaaga atttatccga ctgatccaac agattaccaa
      961 tcaagaacta ccgagtacga cacaagccgg ttatcaaaca gcaaaagagc gaagtatcaa
     1021 taaaatcggt gaagaactta aaaatgtata cgaaagtgta ctgaatgaaa aagtctctag
     1081 caaattaaaa aaagtaaatc aggtcaagga ctagtttgga ggcgaaaaac atgaaaatcg
     1141 gcttttttac cgatacctac tttccgcaag tcagcggagt tgctacatcg atcaaaacat
     1201 taaaacaaga attagaaaga catgggcatg aagtctatat ttttacaacg accgatccta
     1261 atgcggataa gttggaaaaa gatgtgattc gaatgccaag tgtaccattt atttctttca
     1321 aggatcgaag agttgttgtt agaggcatgt ggtatgctta tttggttgcc aaagaactag
     1381 aactagatct tattcatacc catacagaat tcggagcagg gatacttggg aagatggtag
     1441 ctaagaagtt gaaaatacct ctgatccata cttaccatac gatgtatgaa gactaccttc
     1501 attatatcgg taaaggaaaa gtgatcaggc aatcccatgt aaaatacttg tctagattat
     1561 ttgcaaatca tacgacaggt gtagtttgtc caagtgaacg tgttattgat acattacgca
     1621 gctatggtgt aatagcaccg cttcgcgtga ttcctactgg tatcgacatt gaaaaattca
     1681 aacgttctga tatcactcag caggatatca gcaaattaag ggagtcacta ggtcttcagg
     1741 aaaaccattt gatgattctt tctttaagca ggatttctta tgagaaaaat atccaagcct
     1801 tgattaatgg gtttcctcag gtcatctccg cttatcctaa cgccagatta gttatcgtag
     1861 gaaaaggacc ttatgtagaa gagttagagg aattgatctc agaactgcag ttagaagaat
     1921 ttgtgcagtt cacaggagaa gtggacaaca atgatgtagc actttattat aaagcggcag
     1981 attattttgt cagtgcttct acttcagaaa cacaagggct tacttataca gaagcgatgg
     2041 cagccggaac accttgtgtc gtggaaggaa atgcttattt gaaccgattg ttcgatcatc
     2101 cttcattagg gaaaactttt gaaaccgacg aagactttgc accagcactt attgattata
     2161 tttccagtga agttcctaaa gatccaaaag ttttagaagc taaactttat gatatttcat
     2221 ctacacattt cggcgaagct atgatcgaat tttatcaaga catggttgtt tatcatgaag
     2281 aagagatgcg aagaaaagaa gaagaagact cgattgaacg aatcaaaata aaattaaatt
     2341 catttaaaag ataaaattca aacatttttt atgaaaaaat ccttgtttct agcgtatact
     2401 agcatagaag caaggatttt tgatttttag gaggtcgaaa aaatgaaact tggttttatc
     2461 ggaacaggcg tcatgggaag tgctgtcgca cgccatctac tagaagcagg acacgaagta
     2521 gctgtctata atcggacaaa agcaaaagct gatcctttag taacagaagg agctatctgg
     2581 gcagatacac caaaagcggt agcagaacaa agtaatattc tctttacaat ggtaggttac
     2641 cccaaagatg tagaagaaat ctactatgga cagtcgggga ttttttctgc agatatctca
     2701 ggacatattt tagttgattt gactacaagt acaccaagcc tggcagaaaa aattgcaaaa
     2761 acagcaaaag aaaaaggcgc agatgcgtta gatgcaccgg tatccggtgg tgacttagga
     2821 gcaaagaatg gtacactgac gatcatggtc ggtggagaag aagccgttta tgatcaagta
     2881 ctgcctttat tcaaagaatt tggtacgaca tttacattgc atggttctgc tgggaaaggt
     2941 cagcatacaa aaatggctaa tcagatcatg attgcaggta cgatgacagg aatgacggaa
     3001 atgcttgtct atgcacaaaa aaacggactt gatttaaaga aagtgattga gacgctttca
     3061 ggtggaagtg cagctaactg gtctttaagc aattattctc ctcgtatttt aaaagaagat
     3121 tacacacctg gtttttttgt caaacatttt atcaaagatt taaagattgc attagaagaa
     3181 gcagaaaaaa tggatcttgt attgccagca actacccaag cattgaaact atatgaagag
     3241 ttggcagata aaggttttga gaatgacgga acccaagctt tgatcaaact ttggtggcct
     3301 gaaggaaaaa taccagaaaa aaaatcataa aaaacgagtt tttttagaga aaatcaatgc
     3361 aagtaggcta tgcttttgat acaatataac agaaataaga aaaggaggac ctccgatgac
     3421 acattcacaa ttagtcgcaa tcataaaacg actggaggct atggtcgagt cagcagataa
     3481 tgaactgcaa gttcgccgtt ttgaaaaaga aggcgtagag aagtgtacag taacttatga
     3541 taaagcgacc gatacatttg aattaactga aacagatact catcagcaat acgaatttga
     3601 taatatcgat atcgtagcga tggaaatcta tgacttaatc caataaattt taggataatt
     3661 gacatttgaa gttgaagatg cctaaaagaa gccgagaagc aactaattta gttgcttttc
     3721 agcttctttt ttgtttaaca gttcaatttc aaactgttct ttttctaatt ttttgcggta
     3781 actgatcaag atttgtacag gatcgatttc ttcttttgac aattgacgtt tcaaagataa
     3841 atgagcttct ttcttttcat aatagaagtc aggagaaacg tcaccagagg taatcattgt
     3901 ttctgcaagt tgaccataac aaacgaggta gtgctttttc tgactctcat ctgctgctaa
     3961 tggcatgctg aaggtgagcg atggtgttct taaaaactgc ttttgttcct ggatccgtct
     4021 aataaaataa gtaactaatt cgatatcttt ttgaagcata ccggcttttt cgaaattcaa
     4081 ctgatagatg aaatgttgtt gtcttttttt caagtaagtc agtagctttt tatttcggcc
     4141 ttgaaacagc ccttggagtt cctttttttt ctgttcaaaa gtcatttcct gtaggtcagg
     4201 aagctggact cgcaaagcta acaaactaat ttcagacaac caaggcattt gataagtttc
     4261 ttccattata cgcaaaatag acggcatttt tttatagaga cgaaaaggcc cgtatgttcg
     4321 agccgtagga gtatctgtta atacaaatcc cttattggta atatgtacat aattataatt
     4381 actgaaataa ttcatttgtc ggttataaaa aggacggtaa tgttggatca attgacactc
     4441 gagcaataaa gcatctaatt ccgtgtctac tacgacgaaa tcaaaatctg ttatatgatg
     4501 aatcatacgt aatacttttt ttgaatgctg tttattctga tgaaaatagc tgcttactct
     4561 atttttgagc cgttttgctt taccaacata gatgacctta ccatctcggt ctttcataag
     4621 atagacaccc ggcagcatgg ggagagtttt cattttttct tttaacatag gatctccttt
     4681 tacgctttga ataaaaagac tataacaaat tttgaaataa tggacaagtt ttagggaatt
     4741 ctatagttct gttcacagaa aatactttga taatcaaaat atgtgagata gatgatt
//
LOCUS       DAJSBN010000111         4792 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJSBN010000111 DAJSBN010000000
VERSION     DAJSBN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.115"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..867
                     /locus_tag="ONT86_002739"
     CDS             <1..867
                     /locus_tag="ONT86_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2353476.1"
                     /translation="VGDKKVQFTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFG
                     RRSADGLQRERFVLFNQFKIDGKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNEL
                     ESSYSKTMFRLLKQYRTKGIAYFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAI
                     FKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYS
                     EKDPTSLQEKIKEQKRKLELLQQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKK
                     GLLDNLQNLFKE"
     gene            877..1374
                     /locus_tag="ONT86_002740"
     CDS             877..1374
                     /locus_tag="ONT86_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2353477.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            1750..2031
                     /locus_tag="ONT86_002741"
     CDS             1750..2031
                     /locus_tag="ONT86_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353478.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            2033..2449
                     /locus_tag="ONT86_002742"
     CDS             2033..2449
                     /locus_tag="ONT86_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353479.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            3101..3526
                     /locus_tag="ONT86_002743"
     CDS             3101..3526
                     /locus_tag="ONT86_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353480.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            3556..3720
                     /locus_tag="ONT86_002744"
     CDS             3556..3720
                     /locus_tag="ONT86_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353481.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            4678..>4792
                     /locus_tag="ONT86_002745"
     CDS             4678..>4792
                     /locus_tag="ONT86_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2353482.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDV"
BASE COUNT         1758 a          816 c          788 g         1430 t
ORIGIN      
        1 gtaggcgata aaaaagtaca atttacattc gaacaattaa aagatttaag caattataaa
       61 gcaactgcaa atgttcgatt catagatgat ttagaaacaa cgtatgacaa attaatggat
      121 ttacgttttg gtagaagaag tgctgacggt ttgcaacgtg aacgatttgt tttatttaat
      181 caatttaaaa tcgatggaaa agctgatatt ccttttgctg aaatacaagt ccatgagaaa
      241 gccttaccct tacttaataa tcttgaagaa tgggttcgat attctctcca acaattcaat
      301 gaattagaaa gcagttattc caaaaccatg tttagattat taaaacaata cagaaccaaa
      361 ggaattgcct atttttcaaa agaagacttt catgaattac ttgatatccc taaaagttat
      421 agagaaagtg atataaataa aaaaattata aagcctattc gccaagaact aaccgcaatc
      481 ttcaaaggtc tgacaatcaa aaagaaatac ggaaaaggaa gaggaaaacc tgtcattggc
      541 tatcaattca cgtttaaacc tgaaataaaa aaagcagacg actttaataa acacgcacca
      601 acaaaagaag aacaaaataa agctttagaa ctagttaatc aacaaattta ctctgaaaaa
      661 gaccccactt cattacaaga aaaaatcaaa gaacagaaac gaaaattaga acttttacaa
      721 caattagaag agctagaaag aaaacaaaat aatctgaata aagaaaataa ggagattaac
      781 gaacaaatac cagataacaa ttccgatatt cccccagaat taaaaaaagg cttactagat
      841 aatcttcaaa acttatttaa agaataaggg gtttttatga cagataaaac tatcaaagaa
      901 ttagcagata ttctaggtgt ttcaaaagat aaagtgaagt atcaactgag gaaattacct
      961 agtaatttta cctacaaaaa aggaaatatt atctacctga aaaaagaagc agtaacaaca
     1021 attacaaagg ttttatcagg taatgagccg ggtaacttac ctagtgaatt acctgattca
     1081 ttacctaccg attacctact caaggagatt gaaactaaga ataatcatat tgaagaaaaa
     1141 gataggcaaa ttgaaaaact tcaactagct ttagaaacac aacaaaaatt actcgaccag
     1201 caacaacaac tcaccctgca agccaaccaa caaatcgaga aactgcagga acaactacag
     1261 ttgacttata cggaagaatc ttcggaaaat aaatcaacga ctctttctga agaaacagaa
     1321 tcgatagaaa aacaatcaaa gacggagaaa aaatggtggc aattctggca ataaaaccag
     1381 caatttaaat taataaaaat tgctggtttt attcttcttt ttgcttttgc gagcagagca
     1441 acatgggccc atgttgcgat attttggtgg cgccccaatc cccgtttttt cgttgaatct
     1501 tactcggact gcactcgttt tacagctgat ttttctgaat tttgaaagag cataaggaaa
     1561 cgtggtatca ctttcttacg aaaggtgcta gataagctgt gcctatcacg tttctcagaa
     1621 aaaaatttgc tatcactttt ttgccattgt ggctaggcta tgtatagtgt tttggcgaaa
     1681 aatagctata caaatactat atttgtatga tatacataaa ttatatactg atacgaaagg
     1741 aaaatttcca tggacatttt agttcgaaat atcaatccca aaattattac aagaatagat
     1801 aaacgttgtc aggaactaac aaccaaaacg ggcaaaaaat ggagtcgaaa tcaataccta
     1861 aaaattttaa tcgaaaacga ttttgaccac gaacttatga attacaaaaa agatcaattc
     1921 gatttattat tcgaaaaatt tattgccatt caagcagaaa atacgaacat actaaatgac
     1981 tatgttcaca caaacaacca actcattgaa acacttatcg gaggaatata acatgagaaa
     2041 aacttctgtt caaaaacacg taacccttta tcccagtcac gttgacgttt tagattccat
     2101 catcgaaaaa aatccagacg tcaacaatta ttcagaagcc attcgctttt tatgtatgag
     2161 ttatgaacct gctaccaata caaccacaac cagtaaccaa gcaaaactca atgccatggg
     2221 aaaagaaatt tctatcatga cagaaatcat ggcagaattt gctgatttat atctaaaaga
     2281 tactggtgtt cttatcggta caaattctaa agtatatcaa gaagtgaaag aaaaaattga
     2341 aaatcaactc aatccttccc gacgaaatca acccaaaaat cgtcaaaaag aaaccaatca
     2401 aacctttact tctggaaatt ttgacccaac aaactttcta aaaagataaa aaaagaaacc
     2461 gaaaaataaa aaaattcctc gatttctttt tagggatcgg tcttccagca agatttgcaa
     2521 gttggtacca acttgcgaaa aaaaaaagaa aaatccgctt ctgcgaattc ttcttttttc
     2581 attttggggc gctgccctaa tccctaagtc ccggttattc aatagcatgg aaactcaaag
     2641 aataagacgt tccctggaat aaaaaaaagg ctgctcgttc gagcagtcca tacataggaa
     2701 aatgaaaaaa cgacgtccct tactgagaaa cgctattaaa cttttaacaa atcctctaaa
     2761 aaataacaag tctttaaaaa gtgaccggta aaaatgcaca aagaactaaa aaagagaaat
     2821 tgcatcaaat caagcattag aagctagtaa ctagctagac caattgaaaa tcacaaagtg
     2881 agcgcctgta tctattgcta caggcgctac ttggcatagt gtacattatg tcaacaagaa
     2941 tttaatataa catatttttc tagtttgtta taacaatgcc actctaacaa aaatgtaagc
     3001 attatcctta ttaaaagctc cttgacaagg actatacctt ttattttatg atttttacat
     3061 agaaaacgtt ttctctattt tttgtttaga ggtgattttt atgatttgca agaacaagaa
     3121 aaaaattatt gctattattg ctagtttctc tttgattttt agtactgcat gtactgtctt
     3181 attaacaaat aatgctaatg ttgaagctgc aagctcttct tacaaacata atcatgctgg
     3241 ttatagatgt tacaaaatga gttattatgt aagtccttct aaatgcaaac aattggctcg
     3301 ccaaactaat aaacttaata ccgtctcagc tattgcaaac tttattggac tatctggatt
     3361 aacaccgggg attatatcat tagttttcgg atcagcagtt agtgctaata atgtatttgt
     3421 tacagccgct agaagaggaa aaggcgttca attaaattat attgcccatt ttagtaatac
     3481 cactacctat aattatagtt ctaaccaaaa ttatgttata aaataattaa aactttaaac
     3541 taggaggaat gctttatgaa attaacaaaa attgctgtaa ttttagctgt tattgctggc
     3601 attattttat attttgtatt gcctgctaat caattcttcc tttactttgg aatacctgcg
     3661 attttaatag taaatggcgt aagaatcttt gaacaacgaa aaaaatgtag ctctacctaa
     3721 tcgttacaaa acaacaattt caccttcggt gaaataagcg aaactttaca aaaagtggct
     3781 agcaaatgct agcctttttt gtattgtaga gcgtgaattt tgagggggat tcaaggggga
     3841 gcttgcgccc ctttgacaag cggttatttg caccaaatac ctccgtattt ggctagcaat
     3901 ccttctaaaa aatacaccta tcgatcagag tcaaaacaaa tcaattttcg agtgagcgaa
     3961 tccgaatttg caaagctgaa gctttgcaaa tgagtgtgcc ggcttccgta aaaatacata
     4021 tggctataat ttgcgtttta aggcgtttta aacgttctgg tatataatta taccaaaaca
     4081 actttaaaac gccttagatc taattttaga ggtcttatag aacattttag ggttgttagg
     4141 gcttgccctg accgcctgta aggcgctttg agaacaagat aagggtttta tactaagact
     4201 gtcttaaaac ggcttaaaca aacgctcaga acttttcaaa ggagttcttt atgaactgtt
     4261 tttaagcaaa ggagccttta ggcgactgcg cagtataatg tgagagcgta gcgatcacac
     4321 atttttagtg accgtaggga gcgagtttcc cagtttagaa aaattcgcga acttgtttga
     4381 ggaaaaaagc aggagttttt taggcgggaa attttgcctc ctcttttata atctcttttt
     4441 aaacctcttt ttaaacctct ttaccgtcgg ctgtatgctt actctcccaa ggtgtgcaga
     4501 atcttttggg ggtctttttg tctgttttta ggggtctttt tgtctgtttt taggggtctt
     4561 tttgtctgtt tttgggggtc tttttgtctg tttttagggg tctttaatta ggggcttgac
     4621 ccctattaga aagctcttta taattttgtt aagtagttaa tcttgaagga ggattttatg
     4681 gcaaatgaaa ttgtgaaata ccaccatgaa ttaaatacga ttcctttacg caaatttacg
     4741 tcagtagaaa tgaatttatt cttctctata gtatctagaa tgcgtgacgt ag
//
LOCUS       DAJSBN010000112         4743 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJSBN010000112 DAJSBN010000000
VERSION     DAJSBN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.116"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            174..518
                     /locus_tag="ONT86_002746"
     CDS             174..518
                     /locus_tag="ONT86_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR2353483.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="ONT86_002747"
     CDS             519..938
                     /locus_tag="ONT86_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR2353484.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="ONT86_002748"
     CDS             complement(1030..1380)
                     /locus_tag="ONT86_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353485.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="ONT86_002749"
     CDS             complement(1346..2566)
                     /locus_tag="ONT86_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCR2353486.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="ONT86_002750"
     CDS             2932..3498
                     /locus_tag="ONT86_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2353487.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="ONT86_002751"
     tRNA            3643..3730
                     /locus_tag="ONT86_002751"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..4630
                     /locus_tag="ONT86_002752"
                     /pseudo
     CDS             4064..4630
                     /locus_tag="ONT86_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
BASE COUNT         1733 a          796 c          908 g         1306 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat ggaacgagag tataaaatat tttgtgtaaa tgaaaaaatc catacaaaaa
     4681 aggaagtccc ttctgtagaa taaagttaac gacaaccaat tcacagaaaa gaggacttcc
     4741 cta
//
LOCUS       DAJSBN010000113         4712 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJSBN010000113 DAJSBN010000000
VERSION     DAJSBN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.117"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            106..357
                     /locus_tag="ONT86_002753"
                     /pseudo
     CDS             106..357
                     /locus_tag="ONT86_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="ONT86_002754"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="ONT86_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCR2353488.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="ONT86_002755"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="ONT86_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="ONT86_002756"
     CDS             1786..1869
                     /locus_tag="ONT86_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCR2353489.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="ONT86_002757"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="ONT86_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR2353490.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="ONT86_002758"
     CDS             complement(2994..3857)
                     /locus_tag="ONT86_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HCR2353491.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="ONT86_002759"
     CDS             complement(3859..4131)
                     /locus_tag="ONT86_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR2353492.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="ONT86_002760"
     CDS             complement(4148..4357)
                     /locus_tag="ONT86_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HCR2353493.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4712)
                     /locus_tag="ONT86_002761"
     CDS             complement(4455..>4712)
                     /locus_tag="ONT86_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HCR2353494.1"
                     /translation="MIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKV
                     STWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1605 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc at
//
LOCUS       DAJSBN010000114         4646 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJSBN010000114 DAJSBN010000000
VERSION     DAJSBN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.118"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(133..717)
                     /locus_tag="ONT86_002762"
     CDS             complement(133..717)
                     /locus_tag="ONT86_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2353495.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="ONT86_002763"
     CDS             complement(928..1131)
                     /locus_tag="ONT86_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353496.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="ONT86_002764"
     CDS             complement(1141..1563)
                     /locus_tag="ONT86_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353497.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="ONT86_002765"
     CDS             1801..2415
                     /locus_tag="ONT86_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2353498.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="ONT86_002766"
     CDS             complement(2580..3404)
                     /locus_tag="ONT86_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353499.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="ONT86_002767"
     CDS             complement(3428..3583)
                     /locus_tag="ONT86_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353500.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4042..4299)
                     /locus_tag="ONT86_002768"
     CDS             complement(4042..4299)
                     /locus_tag="ONT86_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR2353501.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4292..4561)
                     /locus_tag="ONT86_002769"
     CDS             complement(4292..4561)
                     /locus_tag="ONT86_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2353502.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1532 a          816 c          627 g         1671 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaaggaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     3721 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     3781 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     3841 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     3901 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     3961 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     4021 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     4081 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     4141 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     4201 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     4261 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     4321 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     4381 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     4441 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     4501 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     4561 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     4621 aaatgattaa tattctaact tcttat
//
LOCUS       DAJSBN010000115         4608 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJSBN010000115 DAJSBN010000000
VERSION     DAJSBN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.119"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(83..337)
                     /locus_tag="ONT86_002770"
     CDS             complement(83..337)
                     /locus_tag="ONT86_002770"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2353503.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(448..702)
                     /locus_tag="ONT86_002771"
     CDS             complement(448..702)
                     /locus_tag="ONT86_002771"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2353504.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(895..1170)
                     /locus_tag="ONT86_002772"
     CDS             complement(895..1170)
                     /locus_tag="ONT86_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HCR2353505.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1142..2095)
                     /locus_tag="ONT86_002773"
     CDS             complement(1142..2095)
                     /locus_tag="ONT86_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2353506.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2707..4200
                     /locus_tag="ONT86_002774"
     CDS             2707..4200
                     /locus_tag="ONT86_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HCR2353507.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4335..>4608
                     /locus_tag="ONT86_002775"
     CDS             4335..>4608
                     /locus_tag="ONT86_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR2353508.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          801 g         1541 t
ORIGIN      
        1 cttgaattct tgtgaaaaag ttcttcgtgt tctcttagac ataaaaattc ctcctggtat
       61 gttttcttct agtctacaca ccttaatttt tctgtctagt taattgtagc ctatccatga
      121 tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt aaatttaatt
      181 gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt aataaaactt
      241 gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct
      301 ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta taaaaataat
      361 agacacttct agggatttct aataaccgac acaacaaaga aacagtgtaa tccttacacc
      421 atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg ctgcggcttt
      481 ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt cagctacttc
      541 ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt tgtaaatcgt
      601 tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag tttgattcag
      661 ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc atcctctcgt
      721 agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga tactaacacc
      781 aaatgttgta tacccccaaa caatacacat attttgaagg attaggatta ataaagctaa
      841 aaatatagta agtatactaa atatatttac tatatctagt atacttacta tattttaatc
      901 ttttgatcgt ctattatata taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc
      961 cattgcaagt ttgactattt catacatagg aatatctttt gcataactaa gatcccgaat
     1021 ggtttttagt acgggaggat ctacttgaac tggttttctt tctttcgcag tgtactttcg
     1081 ttcccctaac tcgacattaa tatcatcttt aattgaaata tcttcagcac caattttctt
     1141 cttatctttg ttcgttggta ataatgccat ttagtttaat ctcctttcca agaggtagga
     1201 tatgattggc cattgagtca gcatatttag gtgtataatc aaatccttca atatcaccat
     1261 gttgttcata gtattcaatt ctttcttcta attctgtaaa gatgtctgca aaaatagccc
     1321 accatcttcg atctacataa tcatttaatt ggataccatt ctcaccatac atttctaacc
     1381 gatccgagcc tttaacaatg cttttaaaaa tgtttgattc accatactta tcaattgttt
     1441 tttgatagtt tgcgtgttga gaggaacgtc gcactgagaa aagaacaggc aaaattccaa
     1501 tgatttgaag atgagactct tcaaaattcg tgacaatcgg aactaaaact ttattaataa
     1561 aatcttctgt tccttccatt gcgtaccgtt ttaattcttg cattgcaata atataatctg
     1621 cagcagttaa gaatgcacga accacattat ctgttgatgg tgggcagtcg ataaagatat
     1681 aatcgaaatt ttttctcaat tcttctaaag gtttaatgaa ggccaatgat ctcttacgac
     1741 gatcaggata tttttcggac aagtattcat taagttcttc agatccagga gagccagcca
     1801 taatatataa atgtggactt acttgagtaa tacctgaaac ccagtcccct tttttgattg
     1861 ctgcagacat ggatcgatca aagctagtaa tttgatacgt tgatccaatg ttggaagtag
     1921 tatttctttg ttggtcataa tcaattagca acacgtcttt ttcaaaaagt tgactagaaa
     1981 caatcgctaa taaatcattg tctgtagtct taccaatgcc gccttttcta cctgtaagca
     2041 ttacaacttt tcctagttta tctgatgttt taaatgcagt ccgtaagttt tccatagttc
     2101 cctccaattt cctatactaa atatatttag tatacttcat atacttaata tattcgatat
     2161 atctaatata ctccaaattg ataagaattc agactttata aggaatatag tggatatact
     2221 aaatatattt gctatattca gtatatcaga tatattcaaa atgtcaaaat atatttgtgc
     2281 gttttgcgtt gacaatttag agtgaaaaga ggtaaactta attctataga aaccacagat
     2341 aattcactat ttacaaagta gcaaaagcag tggtactctt taaacagaaa aattacataa
     2401 aaaaaagaga cggaaataat tgattttagc agaatcaatt atttctgaat cacatggtta
     2461 ggacatgtgt tcacgctcgt ctcaagaata tgtaagtagc taccaactac ttatattaaa
     2521 agttgatcgg ataccttttc cgattgcctt tattctacca aaaaaaggcg agcaactcaa
     2581 cagaagaacg tttatttagt gtacttaaat taaatattct taaacactga agagagaaca
     2641 catactatgg ctgagattaa agctaaactg tgttctcttt ttgtttgaga aaggacgtta
     2701 caatcgatgt tgagcgaaat ttattcagct attcttaaaa acggcttacg tacatacaaa
     2761 tatcgaaaca gcaccatgaa acctgcaggt catattgatt ccagtaggag tggtgctatc
     2821 tttgcgtacc gtagcaagca attaatgaat gttgggcgtg gtatggtcat tacttcggaa
     2881 gaagctattt tagagaacga aaagaaacta acgcattgga cacctaatac ctatcgcttt
     2941 ggtacttatg cggatgatta tcgaacagtg gttaaaggtc attctgagaa aaacttaagt
     3001 caaatcaaca catttgtagt tgatatcgat tcaaaagaga accatcaagg cgaaattatt
     3061 ttagcctgtt tagatcaagt gggctacatg ccaacgctaa ttttagaatc agatcatggt
     3121 tatcaagttt attttgtact aaagacacca ggctatgtca caaagaaaag caatttcaag
     3181 gtgatcgatg tagcaaaaaa aatttcaaaa acaattcgtc tacagttggc tgaaaaattg
     3241 tccggagtgg atctaggatg taaccacttc ggaattgctc gattcccaca agaaaataat
     3301 attgttttta aagaaatgaa ctatcagtat tcttttgctg actggattca atggtcgatg
     3361 aagcttgaat cagatcaaaa aacaaaagaa gatcgagaac gaaaactaat tgtctttcct
     3421 gagaaaaaag aaattcgtca agtagatgaa ccgtggttcg atatgttact tcataaagca
     3481 aacatcgtcg gcggagaagg acgtcttggt cgaaataatg tgattttcac gctttcttta
     3541 gcttattact cttcaggtta tgcattggat acttgcgaat acaatatgtt cacattcaac
     3601 gaacggttac aggaaccgct atctgaagca gaattagtaa agattgtacg tagtgcttat
     3661 tctggtaatt atcaagcagc aagcagagaa ttcattattg aattgtgcca gcaatgggtt
     3721 tcaccaacct taaaaaataa tgatctgttc attcaacgac gtggctggtg gaaatttaaa
     3781 aagccacgag atcaaagaaa atatagtcat aaaaatgaat ggaaagaaga tctattggct
     3841 tatctaagcg aaaaaagtct tgctagtgag ccttatttga acctgtcaaa aaaagaattg
     3901 gcttcacaac taacgatgcc caagcgaaca cttgataaga tcttaaatga gttacgagag
     3961 gagaataaag tcttctacaa ggtaacacga ggaagaaatg gcggaatgat cattgcttca
     4021 gtcaaaacgt tgttcgcgag ggttattcaa ctaagtaaag aaaagagact agattattca
     4081 acaaggatca cagaggtatt tggactcaac gagagcttag tagaagagac atttagacaa
     4141 ttatcaaccc ccttgaaccc tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga
     4201 aaaataaaag gtgcaaattg ccttatacaa tatatctatg atacttctgt aggtgttgct
     4261 ccctttgcct agctacgtgt aggtggtata cttataactg cgtatgcagt cttatgtatc
     4321 atggaggaag ttgtatgtcg ttaaaaaatt atgtctattc acatcctgtt aatgtgttta
     4381 tcattggtcg tttgggatta tctgtggaag tgttctgtga gctttatggt tttaagcagg
     4441 ggactttgtc ttcttgggtt accagggaaa aaacagttgc ttctttgccg attgagtttc
     4501 ttcatgggtt gtccttagct tctggggaga ctatggatca ggtatatgat tgtttgtgtg
     4561 tgttagagca ggagtatatt gagtataaaa tagctaacga gttaagaa
//
LOCUS       DAJSBN010000116         4475 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJSBN010000116 DAJSBN010000000
VERSION     DAJSBN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.120"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            323..1054
                     /locus_tag="ONT86_002776"
     CDS             323..1054
                     /locus_tag="ONT86_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR2353509.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="ONT86_002777"
     CDS             1199..3226
                     /locus_tag="ONT86_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR2353510.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="ONT86_002778"
     CDS             3334..4431
                     /locus_tag="ONT86_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR2353511.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DAJSBN010000117         4458 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJSBN010000117 DAJSBN010000000
VERSION     DAJSBN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.121"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..115)
                     /locus_tag="ONT86_002779"
     CDS             complement(<1..115)
                     /locus_tag="ONT86_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR2353512.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
     gene            246..698
                     /locus_tag="ONT86_002780"
     CDS             246..698
                     /locus_tag="ONT86_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353513.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            712..1401
                     /locus_tag="ONT86_002781"
     CDS             712..1401
                     /locus_tag="ONT86_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353514.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1526..3877
                     /locus_tag="ONT86_002782"
     CDS             1526..3877
                     /locus_tag="ONT86_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCR2353515.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3957..4352
                     /locus_tag="ONT86_002783"
     CDS             3957..4352
                     /locus_tag="ONT86_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353516.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1567 a          573 c          885 g         1433 t
ORIGIN      
        1 cacgatcata taagatttct tgaacttcac gatagctaag gttataacga agatagtagc
       61 ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtta
      121 ttcctcctgc tatctttttc tattattcta tcttatttga tagtagattt aaaactttgc
      181 aacagaacca attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga
      241 gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct
      301 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
      361 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      421 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      481 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      541 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      601 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      661 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      721 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      781 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      841 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      901 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      961 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
     1021 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
     1081 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
     1141 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
     1201 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
     1261 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1321 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1381 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
     1441 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
     1501 ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
     1561 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1621 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1681 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1741 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1801 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1861 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1921 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1981 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     2041 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     2101 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     2161 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     2221 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     2281 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     2341 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     2401 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     2461 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     2521 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     2581 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2641 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2701 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2761 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2821 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2881 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2941 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     3001 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     3061 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     3121 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     3181 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     3241 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     3301 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     3361 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     3421 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     3481 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     3541 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3601 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3661 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3721 tgtagggata atttcatttt tcattgataa aatagttaat aattcacttg ggacagattt
     3781 tacttatctt gattttattg gagatgaggg gaaagttgca aatggatttt ctgaaaaaat
     3841 tattcaattt caagaaaata gaagtaaact taattaagaa tcgtttacta tttaaacagt
     3901 gtccttttcc tttttaaata tatacgataa aataagtata tgaattgtaa aaggacatga
     3961 tgattatgga agaaatagaa tggaataatc tgactcaaaa taataaaaat aattctggat
     4021 gtggaattat ctttatgttt gttatagttt tgggaatagt agtactttta ataactgtca
     4081 agatagtaga ttactttgcg aatactcctg aagaacaaat acagagaaaa cttcctgagt
     4141 ttgaaagagt accaccttat gatccaagag ataattcatt tcaagtaaaa aaattaaaag
     4201 agtctccaaa tgaaatgtgg tttgaagtta cagttgaatc aagcgacttc aaagaaaaaa
     4261 agaaagaata caattatatt gtaaaggcaa aagtatcaaa aagatttatt tttaacccta
     4321 aattagaaat atatagtgtt gtaatagaat aatagtgtta aaaaaattgt aaaagttaaa
     4381 gaagtcaaat tctgatgtga aaaataaaat ataccatgga agacataaaa gaaaaactgg
     4441 ataactagtt tatttaat
//
LOCUS       DAJSBN010000118         4433 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJSBN010000118 DAJSBN010000000
VERSION     DAJSBN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.122"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(543..2048)
                     /locus_tag="ONT86_002784"
     CDS             complement(543..2048)
                     /locus_tag="ONT86_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2353517.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ONT86_002785"
     CDS             complement(2045..2731)
                     /locus_tag="ONT86_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2353518.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ONT86_002786"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ONT86_002786"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR2353519.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAJSBN010000119         4425 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJSBN010000119 DAJSBN010000000
VERSION     DAJSBN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.123"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            690..1658
                     /locus_tag="ONT86_002787"
     CDS             690..1658
                     /locus_tag="ONT86_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2353520.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="ONT86_002788"
     CDS             1669..2484
                     /locus_tag="ONT86_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR2353521.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ONT86_002789"
     CDS             2552..3262
                     /locus_tag="ONT86_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2353522.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ONT86_002790"
     CDS             3298..>4425
                     /locus_tag="ONT86_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR2353523.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAJSBN010000120         4394 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJSBN010000120 DAJSBN010000000
VERSION     DAJSBN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.124"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..940
                     /locus_tag="ONT86_002791"
     CDS             <1..940
                     /locus_tag="ONT86_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCR2353524.1"
                     /translation="IDKVFFKLKMNNLNVAMADRERLYNSLQNLTLYSLGDIRDILIL
                     VHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYV
                     KNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQ
                     SRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYK
                     RITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLG
                     LGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            957..1391
                     /locus_tag="ONT86_002792"
     CDS             957..1391
                     /locus_tag="ONT86_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353525.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            2008..>4394
                     /gene="esp"
                     /locus_tag="ONT86_002793"
     CDS             2008..>4394
                     /gene="esp"
                     /locus_tag="ONT86_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HCR2353526.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT         1615 a          606 c          829 g         1344 t
ORIGIN      
        1 tattgataaa gtttttttta aactaaaaat gaacaatttg aatgttgcaa tggctgatag
       61 agaacgactc tataattcat tgcaaaattt gactttgtat tctttaggag acattcgaga
      121 tattctgatt ttagttcatt atttttttac tggaaaaata gaagatctat tccatgaacc
      181 gttaattgaa tatacagaaa atctaagtaa aactatcgag caaatcaaaa tagataatct
      241 gttatcacaa aattacgatc cagaaatata tctgtttctt tatgaaaata aaatactgga
      301 atatgttaaa aatggagata ttcgaaattt agaaaacatg gtttttaatc tgagtaatgg
      361 aataattcca agtgttagtg gagatacgat tcggtctgaa aaaaattatt ctatcattgt
      421 ttttgaaaag ttagcacaaa cgtctataac attaggaatg gatataatag aagcgtatca
      481 aagtagagat gctttgatac aagaaaacga attagctgta tcgttacctg aggtcttaaa
      541 agtgagggat tctgggattg tttattatac taaggaaatc ggaaaaacta agatagagca
      601 tctttctccc ttaatttctt ctgtcgttca attcatcggc ttaaatatct ataaaagaat
      661 tactgtaaag gaaatagcta attatttttc tgtaagtgaa actaaattaa gagaaagctt
      721 taagaatgaa atgcatatta cgatttataa ttatatctct aagcgaaaaa tttctacggc
      781 taaaataatg ttgaaatcaa accatactat cagtgaagtt tctttaggtt taggattttc
      841 agattcttca cacttttcga gagtattcaa aaagtacgca ggtgtatctc ctaagcaata
      901 tcaattaggt ttagtggaca atttcaacaa cattagctga gaggttttat tttattatga
      961 gactatatca tttacgtagt ttggaggagt tgaggaaatt ggattttaac aaaaaatttt
     1021 tttgggaaat ttctgatttg agttgtttcg tagaatctga gcgtctaagt aatggtaata
     1081 atagtgtgtt aaatgaatat gacaaagtag taattaataa agtaaaaagt ttatgtgaag
     1141 aagctagaga atctatcagt catattaatg gtagcaattt taaacaaaca ttatttaatc
     1201 tgattaagct agatgctaaa atatcaagtt atttattctt tttgatacat gatgaattta
     1261 tggattatca aaaattggat cagttaattg aaagagaaag ctgggaagat tattacgtgg
     1321 gtctaacttt ttcagaaaag ttaaacaact acaagttaat agattataag tatttgagtg
     1381 aaagcaatta attattcatg tagagtacta gacactttaa atatggacaa ttcaattata
     1441 aagaaaaaga atcaataatt taatgaggct gtgacaaatg tcattttttg tttcgctaaa
     1501 aacgagtgaa aaaatgttga taactccaat cattcgttta agattctagg aatcattcag
     1561 aaatgctgtg aaaccgatac tttataatta taaagaaggt gatttcaaat tccgaagaag
     1621 gaatttgaag tcaccttttt acttatgtca cagtctcatt cagtaatgcg ggataaggct
     1681 ccttattccg ttatttgaat actctgaagt ttaatctaag aaagggtaat attttgaaaa
     1741 catgcttcat cgcatagaaa tgaacttaat ttcagaaaga atacatacat atctttttat
     1801 atgttttcag ctgctgaatg gttaaaaagc acaaatatcc gaaaaaaaca tgaagaaatg
     1861 tacttttttt gttgtaatgt tcatgaaatt ctctaagtaa attagttaac ataaggttag
     1921 taagattcct attctgtgaa taatacgtat cagatttatc ttttattctt actaggaaag
     1981 taattattaa gggagttgat ttgaataatg gttagcaaga ataataagag agtatttcta
     2041 gaaaaaacaa agaaacgagt attgaagtac agtatcaaaa aattaagtgt tggggttgca
     2101 tctgttttga ttggtgttgg tcttgtcttg ggaacaactg aactagttaa agcacaagat
     2161 gaaatttctc caagcagccc acttgaaaca gcgataagct ccgtacaagt aggtgacaaa
     2221 gtagcttcgg ggaatacttt tcaggagaat cctggttata cgaaaaatta taacttttcg
     2281 gatttacaat ttagtcctca agaactaact ggagacacac taaaaggcaa tacaattgga
     2341 tttgaagttt acggaaaaca taatattgca gcatcaacta aaaactggga aatccgtctt
     2401 caattagatg aacgattagc taaatatgta gaaaaaattc aggtcgatcc aaagaaagga
     2461 attggatctt ctagacggac ctttgtaaga attaatgatt cgctgggtag acctacaaat
     2521 atttggaagg tcaattatat tcgtgcaagt gatggattat ttgctggggc agaaacaacc
     2581 gatacgcaaa ccgctcctaa tggggtaatt acgtttgaaa agagcctaga tgaaattttt
     2641 aaagagatag gcatagacaa tctcaagacc gatcgtttaa tgtatcgaat ctacttggta
     2701 agtcatcaag acgatgataa aattgtaccg ggtattgata gtacgggcta ctttttaaca
     2761 gattcagatg atttctataa tagcttagat gtatctgaaa ataatcctga ccagtttaaa
     2821 catggatcag taagtgcaaa atatgagaaa cctaatactc aaacaaaaga tggttcagga
     2881 tccacaggtg ctaatggagc tataatctta gatcataagt taacgaaaaa ttataatttc
     2941 tcatattcat cttccgcaaa gggcacgcct tggtatgcta attacaaaat tgatgaacga
     3001 ttagttccat atgtagcggg tatacagatg cacatggttc aggctgacaa agtaacatat
     3061 gatgtttcgt ttgaatccgg aacaaaagta gctgatttag caatcgagcg acgggaaggt
     3121 catgaaaatt atggcatagg ttcaataact aataatgatt taactaaact tatcgatttt
     3181 gctaatgcaa gtccacgtcc agtcgttatt cgatatgttt tacaattgac taagccatta
     3241 gatgaaatct tagaagatat gaaagcaaca gcacaagttg aagaaaataa accatttggt
     3301 gaagatttca tctttgattc ttggttgtcg gatacgaata aaaaattaat ccagaacact
     3361 tatggaacag gttattatta tttgcaagat attgatggtg atggaaaccc tgacgataaa
     3421 gaagagagcg gagacacgaa tccatatatc gggaaacctg aattagaaga agtatatgat
     3481 gttgacacaa cagttaaggg gaaagtattc atccacgagt tagcgggaac aggtcacaaa
     3541 gcccaacttg ttgataaaga aggtactgta ttagcagaaa aaactatcgc tccaaatgaa
     3601 aaagatgggg ctccaatttc agatactgta gaatttgaat ttacgggtgt agattcaagt
     3661 aaattaatcg cgaaagatga attaaaaatc caaatcgttt ctccaggttt tgataaacca
     3721 gaagaaggtt caaccgttat taaggaatca ccaaaagcgg ttgataaaca aaccgtggta
     3781 gttggattta aaccagatgc taaagaatca attcggaata ataaaaactt acctgaagat
     3841 gcagagtatt catggaaaac agagcctgat acttctaacg ttactgatag tacgaaaggt
     3901 attgtaactg ttaagatcgg aaatcgaact ttcgacgtgg atgtagagtt tgctgtaaaa
     3961 gcttctcaag ctatggaaaa tgatgcaaca tacgtaccta taacaacaac tccagaaacg
     4021 acagttcaaa gtggaaaacc tacatttgat aaaccagatg ttcctttagc taaagatgcc
     4081 ttttcaattt cagatgtcta taataagtac ttcggtaatg caagtgttga tgcaaacact
     4141 ggtattgtta cattcactcc agctaaaggt gtaggagaat cggagccgat tactggaaca
     4201 attcctatta aaattgttta ccaagatggt tctgtaggca cgaccgattt agcagtaact
     4261 gtaagtaaag atatttatga aaatccagga gaaaacattc ctgcaggcta ccacaaagta
     4321 accttcaccg caggggaagg aacaagtatt gaaagtggaa caacagtctt tgcagtgaaa
     4381 gacggcgtaa gctt
//
LOCUS       DAJSBN010000121         4336 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJSBN010000121 DAJSBN010000000
VERSION     DAJSBN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.125"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(480..1888)
                     /locus_tag="ONT86_002794"
                     /pseudo
     CDS             complement(480..1888)
                     /locus_tag="ONT86_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2109..2471)
                     /locus_tag="ONT86_002795"
     CDS             complement(2109..2471)
                     /locus_tag="ONT86_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353527.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2475..2813)
                     /locus_tag="ONT86_002796"
     CDS             complement(2475..2813)
                     /locus_tag="ONT86_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353528.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(2792..3211)
                     /locus_tag="ONT86_002797"
     CDS             complement(2792..3211)
                     /locus_tag="ONT86_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353529.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3204..3437)
                     /locus_tag="ONT86_002798"
     CDS             complement(3204..3437)
                     /locus_tag="ONT86_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353530.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(3440..>4336)
                     /locus_tag="ONT86_002799"
     CDS             complement(3440..>4336)
                     /locus_tag="ONT86_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2353531.1"
                     /translation="QTLVQTIKLNWSIDRITIVGKLNQNIFYHTQHDVLILDFEQLMR
                     VNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKWQEGKGRIDFNPNKISRFLADSMKN
                     FIHDLFLEPHFSRADVACDLIDVPDEFITQYRIIDPVSFKLIYGRSGKLETVYWGSRA
                     SERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQLRRGKATEWYAMVKESLDSFVSPH
                     FLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYKYRNLLKRESQNDELTNHLRETFEE
                     SADDLKKELDTWLQGLDVTEEE"
BASE COUNT         1241 a          972 c          597 g         1526 t
ORIGIN      
        1 actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag aaaaagagac
       61 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
      121 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
      181 attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt tttttgctct
      241 tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct atgcaaatat
      301 ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta acgctaggtt
      361 ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt agtggtttag
      421 ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc acatcgtaac
      481 taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt ttgacgataa
      541 aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat atccttttct
      601 gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt ttttaatcgc
      661 tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc cacttgtaaa
      721 tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc tacatactca
      781 tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt atcagcattt
      841 ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc attcccagat
      901 aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat atctttttgg
      961 aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc ttcaatcttc
     1021 actctctcag aagccaccag agccttttct cgctcatttt cttctctttg cctagaaaaa
     1081 tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga cgcaattctg
     1141 aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta gtaccgacta
     1201 actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact tcctgattcc
     1261 taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc tgctgttgca
     1321 aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca gcaacaggaa
     1381 caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca ttgtagacgg
     1441 ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt ttgttggcaa
     1501 tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca tcatcacgag
     1561 tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt ggttccaatt
     1621 ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct cttttctgct
     1681 ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca cgaatatttg
     1741 tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta tgccagtcat
     1801 tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta ttctttgttc
     1861 cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt cgctaataaa
     1921 aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta attgtgacat
     1981 ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga caacgaaaaa
     2041 aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc ttcacgcaaa
     2101 ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact ctcactcatc
     2161 tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg ataatctcgt
     2221 tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt ttcgtgttgc
     2281 tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc aaacccaaaa
     2341 cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg ttttttctgt
     2401 tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat atctgctaaa
     2461 gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat ttggtttaac
     2521 tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc agttgtttgc
     2581 gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctag atcagcattg
     2641 ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt atcttcatcc
     2701 ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc gactgtttct
     2761 tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac cattttcgat
     2821 tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga agcaaagtca
     2881 atttttgttg gtcacgaatc atttccgtta attcagaaat atgcctatct ttttccgcta
     2941 attgttcacg caaaaattca ttttcttttt ctaacgggaa atcattgcct gcatcattga
     3001 ccggtaaatc accagtcaat tgaccacctt ttgaccgata aattgaccgc acaacaccaa
     3061 tcaattcctt gtctaacatg ataaccccat tatctttata ccgagcaagg tcataacctg
     3121 cattgaccag ttgattgcgc actgtatttt tagatatacc caactcatca gcaaggtttt
     3181 ttatggtcat tttttctggc aaatcagtca ttatcttctc cgttactttc agctacccca
     3241 taaacatatt taacagcatg cttagggata atgattgggg aatcaatttt attatttttc
     3301 aaaatcaact catactgcgt tacatcaagt aacttaccgg acattttttt gctatccaaa
     3361 aaaacaacga caacgttatc acctgttaaa ttcaccaacc aatttttctt accaaaatgc
     3421 ttttgttttt ctgccattac tattcctcct ctgtcacatc aagtccctgt aaccaagtat
     3481 ccaattcttt ttttaaatca tctgctgatt cttcaaatgt ttctcgtaaa tgattcgtca
     3541 actcatcatt ttgactttct cgctttaata aatttcgata tttatatttc gagtttcttg
     3601 acaaaatcgc ccaattagaa tgattagcta acaatccatc aatcataact ttatccgtag
     3661 gttttatatc agttggtaaa aaatgtggac tcacaaagct atccaaactt tctttcacca
     3721 tagcatacca ttccgtagct ttaccacgtc ttaactgcat ttctaaccgc caccaagtat
     3781 taatttctga aggcaccacc tttcttttca tttcttgctc tattttttta ttgtacaaac
     3841 gaatttgccg ctcgcttgcc cgactacccc aatacaccgt ttccagtttt cctgagcgtc
     3901 cataaattaa tttaaatgaa actggatcaa taattcgata ttgcgtgata aactcatcag
     3961 gcacatcaat aagatcgcag gcaacatccg ctcgtgaaaa atggggctct aaaaataaat
     4021 catgaataaa atttttcatg gaatccgcta aaaatcgact gattttatta ggattaaaat
     4081 caatccgccc ttttccttct tgccatttag aaatttcaat ataggcaata ttttcgtcat
     4141 gttggtctaa tagctgccaa ccattatttc caaccgcttt taaatagcca ttgccttcat
     4201 ttacccgcat aagctgttca aaatctaata ttaatacatc atgttgagta tgataaaaaa
     4261 tattttgatt caactttcca acaatggtaa ttcggtcaat cgaccaattt aattttatag
     4321 tttgtaccaa agtttg
//
LOCUS       DAJSBN010000122         4310 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJSBN010000122 DAJSBN010000000
VERSION     DAJSBN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.126"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(65..1285)
                     /locus_tag="ONT86_002800"
     CDS             complement(65..1285)
                     /locus_tag="ONT86_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCR2353532.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1916)
                     /locus_tag="ONT86_002801"
     CDS             complement(1305..1916)
                     /locus_tag="ONT86_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR2353533.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="ONT86_002802"
     CDS             complement(1959..2891)
                     /locus_tag="ONT86_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR2353534.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="ONT86_002803"
     CDS             complement(3312..4172)
                     /locus_tag="ONT86_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2353535.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DAJSBN010000123         4203 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJSBN010000123 DAJSBN010000000
VERSION     DAJSBN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.127"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(1..354)
                     /locus_tag="ONT86_002804"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="ONT86_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="ONT86_002805"
     CDS             complement(614..1162)
                     /locus_tag="ONT86_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353536.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="ONT86_002806"
     CDS             complement(1163..2020)
                     /locus_tag="ONT86_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2353537.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="ONT86_002807"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="ONT86_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="ONT86_002808"
     CDS             complement(2582..2911)
                     /locus_tag="ONT86_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR2353538.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2988..3260)
                     /locus_tag="ONT86_002809"
     CDS             complement(2988..3260)
                     /locus_tag="ONT86_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR2353539.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3260..3523)
                     /locus_tag="ONT86_002810"
     CDS             complement(3260..3523)
                     /locus_tag="ONT86_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2353540.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(3629..4045)
                     /locus_tag="ONT86_002811"
     CDS             complement(3629..4045)
                     /locus_tag="ONT86_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353541.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4051..>4203)
                     /locus_tag="ONT86_002812"
     CDS             complement(4051..>4203)
                     /locus_tag="ONT86_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR2353542.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1225 a          851 c          533 g         1594 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaaca
     3061 acccggtgtt gtctattcaa tcttctagaa tacctacctt catgtgtagg ttttaacttc
     3121 tcaaatgatt gggtagggag atagggatct tcctttaaag tctgaataac cttctcaaat
     3181 tgaggtttca aattagactg tttgattttc tttaaatcag ctttagccga gtttttaatc
     3241 atcacagaat agttattcat tataagttat cccaatcaat ttcatcaaca ttagtaaatc
     3301 cactatcatc tttttctcgc ttacgaacaa cacccaaagt gcctatttgc tcaagtaata
     3361 gcgtttcttt gattgctttc caatcatcca aaccaataag caccgcatta ttttcactgc
     3421 gctcactaat gatttcaatt tccgtatgat tctcatttac atctttcaac aacttataaa
     3481 aatttttccg tgctgagcta ggatttaaga ctgtttttgc catatctact ccccctctaa
     3541 tttatatctc cattctaacg tacttaatta agtacgtaaa ttcgattgtt ttgtttaaat
     3601 tggagtacca aaacagcatt gtaaagtgtt atccgtttcc tttattttta ttaacaagtt
     3661 ttatcacagg cttcatatat ttgattataa cttcatcttc tacgtagata tctgttatca
     3721 ttacgacatc tgtactattt cgattgagta ctttcacttg atctccagca ttgattttcc
     3781 ctctaaatct tttaggaatg aaccaactat acatatgatt agcatcacca aaatgtgctt
     3841 ttatcgcatt ctcaggatat aacttattca atagttcatt ctttttaatt ttcaaaactt
     3901 caaatctata cttatcatca tctgctaatc taaataaatc ttctaaacgc atgttttcta
     3961 actgtaagtt ttgaaaatct tccggtataa attttcggtt ctgttgcgaa gtttgaaaat
     4021 caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt attcccatta ataccttgat
     4081 ttcagtagac accgaaaagc tgaagagcgt tccatttctt cggttctttt tatatattcc
     4141 tcgaatggtc tccatgccct taatcgtgga agaggctgta cggagacttt gataaaattt
     4201 att
//
LOCUS       DAJSBN010000124         4194 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJSBN010000124 DAJSBN010000000
VERSION     DAJSBN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.128"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1008
                     /locus_tag="ONT86_002813"
     CDS             <1..1008
                     /locus_tag="ONT86_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353543.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="ONT86_002814"
     CDS             1025..1174
                     /locus_tag="ONT86_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353544.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="ONT86_002815"
     CDS             1171..1617
                     /locus_tag="ONT86_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353545.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="ONT86_002816"
     CDS             1619..1756
                     /locus_tag="ONT86_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR2353546.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..2087
                     /locus_tag="ONT86_002817"
     CDS             1794..2087
                     /locus_tag="ONT86_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353547.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            2084..2308
                     /locus_tag="ONT86_002818"
     CDS             2084..2308
                     /locus_tag="ONT86_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR2353548.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2305..3330
                     /locus_tag="ONT86_002819"
     CDS             2305..3330
                     /locus_tag="ONT86_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR2353549.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            3520..3593
                     /locus_tag="ONT86_002820"
     tRNA            3520..3593
                     /locus_tag="ONT86_002820"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3554..3556,aa:Ile,seq:cat)
BASE COUNT         1450 a          719 c          842 g         1183 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa actttattta gggagagaag aaaatgaata ataaaacgtt
     2101 cgaagtacta aaatggttcg cactggtaat tattcccgca ctagctactt tcgtggggtt
     2161 agttggtaaa gcgctcaatt ggcagtacac agatatctgt gttgtcatca ttactggttt
     2221 tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga acctacaaaa tgttctcggc
     2281 tgaaagcgaa gaaggaggaa acaaatgaaa aagaaaatta ctattactgc gatgagcctg
     2341 ttaacggctc tttttttatt acccattaat acgtttgctt atactattaa tgacgagtat
     2401 aatttagcgc cgaatcaagg agactccaga ttagcaattc ctaacaaaat tattttgcac
     2461 gaaactggaa tagatgcacc agcaagaaac gtagccgcca acatgaaaaa taattataac
     2521 ggaagcaatt cttatactac agatgttatt ggtgacggtg ggattgttta ccgtgtgggt
     2581 gagcaaggat atgtttcgtg gggagctggt aacgctaatc cttatgcgcc tgtacagatt
     2641 gaattacagc gcacatatga taaagcattg tttgaaaaaa actatcgagc ttatattgaa
     2701 tatacaagag atagtgcaaa aaaatatgga attccattga ctcttgatca aggaacttct
     2761 ttatttacaa aaggaatcat ttctcatttg tgggtgacaa attatgtttg ggggaaccac
     2821 acagatccat atggttactt atcgcaaatg ggagttagca aagaaaagct tgcttatgat
     2881 ttagctcatg gatttaccga tgaaaatcca acaacttctg aaaacaagcc tgtcattgat
     2941 ccaacacgag ctggtgcagc taatcctacg ctgacagatg gaacaaatca cgcccacatt
     3001 gatcagtttg gggaaatcga aaacgcaaac ttgcatgtgg ctggatggca cattgctaac
     3061 tataaatacg agtatatttt cattatggac tacaatactg ggaaagaatt agctcgagta
     3121 agagctgatg gaatttatag accagatgta aaccaagctt ataatacttc tggaaatgtt
     3181 ggttatcatg tatctttcaa tatgcgtaat tttcctagta agaaagtcta tgtaatgatg
     3241 cgggcaacga atgatccaga gggaaacact aaaggcggtg cgcaagattt ccatgacaaa
     3301 cgttggtatt taaatattcc taaacgataa aaatagctcc tcgttgagga gcagtacata
     3361 actatattga caactataaa aattattcga taaaatagtg atgttatcgc atatcttcac
     3421 tatcacccat aaatagtcac actccaagct atgcgataac aggtttgttg ccacacattc
     3481 tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc tcagttggtc
     3541 agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt acattaacgt
     3601 agccatttga atcgttctgt gttagaattt ttttgaagag tattatacaa gctaaagctt
     3661 ttcttcattg ccactcaaat gagtggcttt tttatgtatc cttttatgga ttaatgaaag
     3721 gatgtttcac atagttatac ttctgtatat ttgaaaagtt ttactttgat ttttaaatag
     3781 aaagacattt gggttaaatt gtgagataat aataaagaag agtttaaagc gcaccccaaa
     3841 ccacttcccc ataagtgtgt tacgctttaa actcttttat atttgaagcc attaaaaagc
     3901 ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca attggagtgg tagttaatta
     3961 gtgacttatt tttgatttta tagcactgat actataaaat atagatatca tcatattaca
     4021 caatcttaat actaacttaa aaaatatctc ctttcacaag tatggtgata aaattcgttc
     4081 cgggctacct ttttaggtag cctactttaa tctttatacc tttctggatc aacgaaagta
     4141 tactttatat agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc agtc
//
LOCUS       DAJSBN010000125         4036 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJSBN010000125 DAJSBN010000000
VERSION     DAJSBN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.129"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(242..391)
                     /locus_tag="ONT86_002821"
     CDS             complement(242..391)
                     /locus_tag="ONT86_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353550.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="ONT86_002822"
     CDS             853..1065
                     /locus_tag="ONT86_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353551.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="ONT86_002823"
     CDS             complement(1343..3142)
                     /locus_tag="ONT86_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR2353552.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="ONT86_002824"
     CDS             complement(3266..3862)
                     /locus_tag="ONT86_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR2353553.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttttt
//
LOCUS       DAJSBN010000126         3861 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJSBN010000126 DAJSBN010000000
VERSION     DAJSBN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.130"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..132)
                     /locus_tag="ONT86_002825"
     CDS             complement(<1..132)
                     /locus_tag="ONT86_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR2353554.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            543..3692
                     /locus_tag="ONT86_002826"
     CDS             543..3692
                     /locus_tag="ONT86_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCR2353555.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1356 a          654 c          808 g         1043 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg gagggaagaa
      541 gaatgaaaat tagtcaccgt gaggaagcgg aagtcgaaga acagttgatt cgtgtcctcg
      601 gggaaggaca taatcagtgg acgtatcgtc cggatctaaa atcagaagaa gatctttgga
      661 gcaatttacg tcaaaaaatc atctcaaata atcaagcaga actgaatgat tctcctttga
      721 ccgataaaga gtttgaaacg atcaaaaccg aattattgct acggacgaaa accccatttg
      781 atgctgcgaa atggttaaaa ggtgaaaatg ggatggctcg gatcacgatt gaacgggaaa
      841 atccgcaatt gggttctgtc tcacttatcc tttattccaa tcaagacatt ggtggaggaa
      901 tctctactta tgaagtggtc catcaaattg caaaacgtgg aagtaacata gaagcgcggg
      961 atcgacgttt tgacgtcacc ttattgatca atggcttacc gattatccaa atcgagctaa
     1021 aacaagtaac tgcaaaagat ggtttttatc aggcgtttaa tcaaatcaaa aaatatgctg
     1081 aagaaggcat gtttcggaac aatatctttt cgaccttaca gttgtttgtg gtttccaatg
     1141 aacaaacgac tcggtatttt gcaaatgcct taccgaagga tttgcatcct aagtttttat
     1201 ttagttggcg aacgaaagat aataaaaagg tagaaaactt atatgagttt tgcaaacaag
     1261 tgctaaatat cccagatgcc catcgcttga ttgcggatta tacgattgtc agtgaagatc
     1321 aagacaataa aaccctcatg gtgttacatc cttatcaagt gcatgcgatt caagccttgt
     1381 ttattgccgc aaataaacat caatcagggt atgtgtggca tgcgactggt tcaggaaaga
     1441 cgttgactag ttttgtatct actaagcttt tagcacgaaa atcggggatc gatcgaacga
     1501 tcatgttagt ggaccgcaaa gatttagata atcagaccac cacagagttt acgaaatttg
     1561 cttcggagtt taatactggg gtttcttctg gtaatgccaa agcgaatagc ttgatcgtgg
     1621 gaacaggaag tgccaaagag ttaagtgaaa ctctgttggc agatgccaat gcgaatgttg
     1681 tgatcattac aacccgtcaa aaactagatg cggcgttgaa atatgcaaag aaacaggaag
     1741 agaaaaaagg aaccaatcgc ttccaaaaat taatggggca acatatcgtg tttgtagttg
     1801 atgagtgtca tcgagcgtta agtgctgaaa atatggaaga aatcaaaaaa atgtttccga
     1861 aatccacttg gtttggcttt acaggtacac cgattttccc tgagaatcgg aaacaagcca
     1921 aagggcaatt ggcacgtacg acccatgatc agtatggaga ggtcttgcac acctatacca
     1981 tcaagaatgc attagaagat ggctcggttt taggatttca agtagagcat gaaaacaccg
     2041 tggagccgac ttcattagag aataaaatct atcggaaatt aaaagaagta gaaacatatg
     2101 cagagtattc accggaacag atcaatcgta tgatcgatca aatggagtct gtcaaaaaag
     2161 aaagttattt ggaccctact gtttttgaag cagaggaaca tatccaaaaa gtcattcata
     2221 aaatgttccg tccagacaat gcctatacaa aatttgattt tcgaaatggc cgtccaacga
     2281 agtctgcgat cttaaccacg agttccattg atatggccaa aaagtattat cgggccatca
     2341 aagagatgac gaaagaatct gattggttaa taaaagagtt tcccaatcag cctatccgtg
     2401 aaggacgaac gatggaggat cctgatttcc cacgaatcgc tattacctat tcattagaag
     2461 aaaatagccg agattcaagt gcccaacaag aagaaatgca aaaaattatt gaggaatata
     2521 atcagtatta tgatactgcc tggtcactag cagacattga gcgttataac ggagacatca
     2581 ataatcgttt ggcacgaaaa agagcggaat tcaaacaatt tgggaaacaa atcgatttag
     2641 taatcgtagt ggatcgtcta ttgacaggtt ttgacgcccc tacgattcaa accctatttg
     2701 tggatcgtaa tctagagtat gcaggattga tccaagcctt ttcacgtacg aaccgtacgt
     2761 atcctgaaaa aacaaaagga ctaattgtta ctttccgaaa gccagcgaca atggagaaga
     2821 atgtcgagga tgcgacaaaa ctgtattcag aagctaaaga agaatcgggt ttgatttatc
     2881 caagttacca agaatccaaa aaaagattca agcaagcata tggtaagtta aaagaggttt
     2941 catccatgga atccattgat gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag
     3001 catttcaaga attgaatcgt gcgtatgaat cgttagtgac ttatgatgat tacaatgatg
     3061 acatggtgca atcttcaact ttgaacaatc aaattcaact tttggaagaa caaattggcg
     3121 tgtacgagac cgtgaagggt tcattgatgg aggaagaacc agaaaaagag ggagaagatt
     3181 tttcaacgat cgaattttat agtgaaaata ccactaagtt gtatgatatt gattctactt
     3241 atattgatca attactagga acttatgctg cgaatagttc agatgcacga gaagaaattg
     3301 aaaaagcttt ggctaaattg aataaaacag aaggagtcaa agaagtttac cgacaaattt
     3361 taaatgcatt tgatgtgggg actttagata gtaatgaaga tatctttgtc attaaacgtc
     3421 gctattttac acaagcaagt gataatttga ttcatgcctt ttccaatgag tggtttgttt
     3481 ctgagaaaga attacatgct tctaatctgc aatatatgcc aggtgaagat ccaattccta
     3541 atatgaaagc aattatcaac agtaaggatt acgaaggata taaagccaaa catccagaag
     3601 cgaaaccatt taaataccca caagaaatga agcgagcatg gcgtaaaatg ttagatgatg
     3661 agttgattcc acttgaaaat gaattgaggt aaacgagaag agttaagaaa attgtacgat
     3721 tcaaagatag tagtaaagtg gaagtgtaga aaatagggat agatgtcttt ttacttgttc
     3781 acattttata acggagtatt taagaaagct attgaataat gattcttcca tctttttcat
     3841 tcatttaccg gttctgttgc a
//
LOCUS       DAJSBN010000127         3714 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJSBN010000127 DAJSBN010000000
VERSION     DAJSBN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.131"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ONT86_002827"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ONT86_002827"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR2353556.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ONT86_002828"
     CDS             complement(2261..2512)
                     /locus_tag="ONT86_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCR2353557.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ONT86_002829"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ONT86_002829"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCR2353558.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ONT86_002830"
     CDS             3462..3680
                     /locus_tag="ONT86_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353559.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1026 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aaga
//
LOCUS       DAJSBN010000128         3691 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJSBN010000128 DAJSBN010000000
VERSION     DAJSBN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.132"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            440..1480
                     /locus_tag="ONT86_002831"
     CDS             440..1480
                     /locus_tag="ONT86_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2353560.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2304..2636
                     /locus_tag="ONT86_002832"
     CDS             2304..2636
                     /locus_tag="ONT86_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR2353561.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3255..3596
                     /locus_tag="ONT86_002833"
     CDS             3255..3596
                     /locus_tag="ONT86_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCR2353562.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1359 a          483 c          682 g         1167 t
ORIGIN      
        1 tggtagtact tattgtattt tttagtagta tataatctat tgttttctag cctaaagcct
       61 tgtcttataa gaataatata atatatacta ctgaaataag gtagtatata ttactagtaa
      121 tggtagtatg ttttatatgc ataaggtagt tcttaattta ttttttgttt tgacactggt
      181 agtacttaat tatctttaat tttaaaacta aattttattc atacgttttt ctggtagtat
      241 ttaattataa ataatattta tttaaagtag ttcttaatga ctttaaaggt agtacataat
      301 tcattttatt tctgatttta ggtagtattt aattaaaaaa cagttttaat gcgtttgcat
      361 tttttattta tctttgctat tttcgaaaag agtaggtaat tttacataaa taaaacaacg
      421 tgaaagtaga ggatctacaa tgactgattt taaatttttt aaggcggata gagtttacaa
      481 cgaattattt tatcaatttc caaaagtctt tattgtttct gacgaataca aaaaaatgaa
      541 agattcaact aagattgcct atatgctttt aaaagcaaga ttagagatcg caatcagcaa
      601 acggcaaatc gatgaagaag gtaatgttta ttttacttat acgacaaatg aactatgtag
      661 agtattaaac tgccaaaaac aaaaagcgat agcaatcaaa aaagagttgg aatcctttgg
      721 tttattatta caaaagcaga tgggatttaa caaacagtta gggaaaaata atcctaatag
      781 actatatcta gcagaattaa aagtctcaga aaatgatatc tacttactcg aaaaatttga
      841 tagagagaat agggaaaacg ttgataaatc agagggtatg aaaatcatac ccaccctcga
      901 cgaaaaatca gacgctgaat cccttggggc tcaagagggt atgaaaatca taccgtgcca
      961 aaacgttgat aaatcagagg gtatgaaaat cataccagaa cttaataata atatattaga
     1021 cactaataga cacaatatag acactgaaaa agaccgccta caagatcaat tgttgttaga
     1081 caattttgag acaattatga caaacgacag cattgctacg tttgtccctg aacgagtatt
     1141 aaatttgata aaaacatttt cttcaagtta cagtgaagct caaaaaaccg tccagactat
     1201 tcataatgca aagaaaaaag ctgaaataga aagtggtatt tcgatagttt ttgaagaact
     1261 cgatagttat tatgtcaatg cagaacaaga attatacacg acactgttaa aagcctatca
     1321 aaaattaaaa accgaaaaag tcgaaaatat ccagaacctg atttttgtct atgtaaaaaa
     1381 ttggtttatc gaaaaaccaa tagctgctaa agtatcaagt gaaaaacgtt tgaattatga
     1441 aagctcccca agcactatta cgaaagactg gttagagtga aactttactg acaaagtgat
     1501 tgttgcttca tggagtaaca ttaaaattaa aaatttttta ttattctaat tatattacga
     1561 atattacaaa ataatagcta ttttaataga atttataagt taaaatatta tgataaaaat
     1621 cacaaacgta gccttttacg tcataagtgt taaaagaaat aattaatttc aaatatagaa
     1681 aagggaacgt atgtttgcat actccttttt tttgagttat aataatacgc gaagagagat
     1741 aatttcatta tttaaacaag ctaaaaatct tgatattact tcttttcagg caatacgaat
     1801 attattcgct tacatttttt gtataaattt aattgtctgc tatgcttgtg atgaattgat
     1861 tttcgttctg cacttttgga tgataaattt ggacaattga aaatacaaaa aaaagaaagg
     1921 cggtatgaac tcggaaggct ggtaaccttc gtcagcgttc atacacggca ttaagatttg
     1981 gcccttaatg cgttaacctt tcagtgttac ggtattaccc gcaatggatt tagtataaca
     2041 gatattcaaa agtgatacca ctttttttgg ctgtgttaag gttaaaaata cgtgatatta
     2101 cgatgaatct atgcctaagc acaatgttat aaaaatgtcc gtgtggctct ttgccgtatg
     2161 acgaacgctg aaattagagg gaacgcatgt ttggatgcgt tccctctttt ttgtttgtca
     2221 tgaacgaaaa tcaattctct atttttgaaa aataacaaga aaaacttgtt aaaaatattt
     2281 aaagaaattg ggtggtttat tttatgtcat atacaggaat tttgagtctc aaagatattt
     2341 gtcactacgg taaacggtgt accgctacag agaaaatcac gaagaaacta tcaactggcc
     2401 aaagtaaaac cgttgtgcag tgtaagaaat atataatcca gaaagacaaa gtctcagagg
     2461 aaatgattta ttacattggg aaacaaaagc aaataatttt aaaagatcct atccccttaa
     2521 aagagctata tccaacaatc aaacatgttt atgaccaaaa cggtgtactg attggtcgaa
     2581 gaaaaaatgg agtcttgcgt tgtaccgcaa aagggatggg gcgtttgatc ggctgaattg
     2641 atcattctca taacttgtat tttatcgttt ttcgcttgct tgaaaatatt gtcgagactt
     2701 ctaaaggaac ttagagtcac tagaaaaata aggagtagac ttgatctatc aacaattccc
     2761 ttcggggagt gacggggtag gttttaacga ttggtaacgt aaaaaattgg tcaatcgagt
     2821 aaatgcgctt gtttggatag tggtgaaatg aatttgtgta aataataagc gcagagtaaa
     2881 caagagggaa tttagaagtt agacaagtta gaatagcgtc tagattaatc atgagcagct
     2941 actaagggat ctagactttg acgcaaactc atggaataag gagtaggtct tctcgtaatg
     3001 gggaggacgc acaagaaatt gtgtcgtgtg ctgcagtaca tgtagatact tgcgaggacg
     3061 ttgattattg gatcaatcga gcatttaccg ctgtctacta gggtttctcg tttcggacgc
     3121 cataaacgag tcgaaatttt ttatgtattt ttaagtaaac gaaaaacgat aaaatatgaa
     3181 caagtcctga ataaagtgaa aaaattgata taaaacatag agaaaatgaa atttacgtaa
     3241 tggaggaatg acaaatgaaa aattatgatc cgaatatacg attgggcaca catactatta
     3301 aggtttcttt ccaacgttgg gattacaaag gatttgtgac gtttagaaga ggcggaaatt
     3361 gtaaaggatt agatgtttta gcattagacg aggatgattt atacgatcaa acattgactg
     3421 ataatccaat tggattcggc ttgctacctg aagatgatga aggtaatgaa tggtttaaaa
     3481 tgacgcttat gaatgataat ggagacgaat tatctgttga agatacttgg tcatatttga
     3541 gtgattatat tgtcagcttt gaaatcattg aatttgtagc ggataaagaa gaataaaaac
     3601 tggaggaata aattttacag ataaaggctt agaaggagtc acgatgatta ataaaaaggt
     3661 agtattacct taggcattga gtaaggaatt t
//
LOCUS       DAJSBN010000129         3495 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJSBN010000129 DAJSBN010000000
VERSION     DAJSBN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.133"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1533)
                     /locus_tag="ONT86_002834"
     CDS             complement(<1..1533)
                     /locus_tag="ONT86_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2353563.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKI"
     gene            complement(1552..1956)
                     /locus_tag="ONT86_002835"
     CDS             complement(1552..1956)
                     /locus_tag="ONT86_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCR2353564.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(2188..2445)
                     /locus_tag="ONT86_002836"
     CDS             complement(2188..2445)
                     /locus_tag="ONT86_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR2353565.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(2570..2902)
                     /locus_tag="ONT86_002837"
     CDS             complement(2570..2902)
                     /locus_tag="ONT86_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353566.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         1088 a          644 c          482 g         1281 t
ORIGIN      
        1 aatttttgtt aaataaatat cccctatttg aataaaacga ttgactaatt gaacttctcg
       61 actacaccaa ctattaatat cattgatgaa ggaatataat ttatcgtccg atgttaattg
      121 gatttgataa atccaagaat tcaaaaagat agacgttatt tgaagataat aatagtcaaa
      181 ttctatttga aaatctctct ctatttcctt aacatactgt ctaaatcgaa tctcttgata
      241 ggtgggatat gttaaagctg aattattttg gatatcaaca ttcttttgtc taaaatatct
      301 atcatatttc tcaaaaagct gctgcttatc agtgtctttc ttccacgttt gataaagata
      361 ctctataagt tcgttgatat taaaaacttc gtctactttt tccaaatcat ccatttgaat
      421 atattttaat tcattctgtc ttttcataac cttcatgccg ttgaaattca cccattgtgg
      481 taaatgatac atctgaatat aaagttcttc caaatcatct aagaaataaa aggcattggc
      541 acaggtcaat tgaatgatat ttttcaaatt gccaacattt tcactaaagc gatagtttaa
      601 taaaaaatct aataattgtt gagatatcag tattttcttt gctagtcttt cttcttctga
      661 tttaaaaaat atcttgatta actgttttct ttcataaaga gaacgactgt ctaatgttgg
      721 aagtgttagc tttacaggta ttcttctcaa caaagttgat aatagtttat cctcaggtgg
      781 ttctgatgtt gcaaatataa tccagcaact tgatgtgtac cagttttcat tatcacccat
      841 ccgatgaaat ttcctcgtat ccataaactg aaaaagtttc tcctgacatt cattgctcaa
      901 tgcatgaatt tcatctaaaa ataagactcc attattagct aattctgcta aaccctttct
      961 tccatcttcg gctcctgtat atgcaccttt tacatgccca aaaaaattgg acaagaaaag
     1021 ttctggatta ttggcatatt cactacaatt aactgaaatg aaagatgcct tctcttctat
     1081 ataattattg gccaacaaat ccttatacac ataatttgct aaatagcttt ttccagttcc
     1141 agtctcgcca tgaagtaaaa tgggtaagcc atttggcgga tagtagattg ctgctttaat
     1201 ttgagcaatt acatttcgca gacttccttg acttccgata accctttcta ttccaaccct
     1261 aaaagagcta tcataccatt tttccgcttc ctctgaagag ctaaaaattt gttgatttgg
     1321 aactttctcc ggtaaagaaa agaagattac tggacgagta ttgatttttc caaattctcc
     1381 atcttttacg aattcattca aatattgaga gacaatatta cgactaacat ttaatttttc
     1441 agatattttc tgtgctgtat tgttccccca tttattttta atctcagagt tctctatgac
     1501 ataattgaca atgcgttctt ttaattcttc catcacagca cctcctcgac actattcgaa
     1561 ttttgccgac agtatttttc cataaatatg acttgctaaa aaggctttgt ttagatcatt
     1621 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat actctggcga
     1681 aatcgcatac cccaaaatat caacgattgt tgcaatcaga tcttcttttg attcttcatc
     1741 tgaaagtaat tcacgataca acatcgtttg tttgcttact gtgtcatatt cctctaagga
     1801 ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt tatagaaaat
     1861 cagccccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
     1921 cgcactaaat aacttctgat ttaattctgc acccaaaaag atacactcct atctcttatt
     1981 taaagttgct atataccctc ttattttagc atactcagtt ttagaaaaag caaaaaaata
     2041 aagaaactta ttcttgataa caagctacgt gacgtatatc cctaacacaa aaatagccat
     2101 atcctttggt ttggatacgg ctatttgata ttactcattc aattatcaaa tcacagtagg
     2161 tgtgaatctc atcgggatcg gcaatatcta cttctgatta ggatcaaagg tgattttctc
     2221 aataaaggtg tgatacaaag ctttaacttg cttcttctca ctctggataa gaaagcaatc
     2281 caaactagtc aatatctgtt ggacatcttt gacttgaatc ggttcccctt gttgttgaaa
     2341 gatccctaag atttcattct ctcgaatttg taactgtctt cgtttttcag tcaattcatt
     2401 taaacgatct tttaacatcg gaaacaattc gggactatct tccaaagcag cttcctgttt
     2461 ttgctctaat agttagattt tgcgtaccat tttcttcttt tattttcctt cgattgctga
     2521 ttttaatata gtctaaagga aatttatatt tattgtattc tttataactt caagagaact
     2581 tcgtttgagt attactgtat ttttttaatt ccataagaaa attagttatt acctgccacc
     2641 taatcagcaa atctgaacct gagtcaatat aattaatatt attttccatc tccaacattg
     2701 taagtactgt tggtgttaat atagggactt tgtacaattg tatttttttg attttatcat
     2761 ctgcacaaag gagatttttt aaatctaatc cttcttttat ttcaaaagtt gaatttttta
     2821 aatctataag agtaaaacca attccttcat actgaatttc tgaaaatcga atttttctcc
     2881 cagttgatag atcaaggggc agaaaacctt ttattacatt gtgagtgtca ccatcattta
     2941 caatgtcatt aggaattttg attttagaat tctgtggagc acctattgga agataataat
     3001 tcattataat tttgtagtct tcatgaggaa ataaagtact tttaggattg ctaagatatt
     3061 ctcgtaccga atctaagaat ggcttatcca ttgaatcttc atccccattt aaactaaaaa
     3121 acattcctac aattgctttt gcaactctaa gtgaattcat tttctttatt gaatgtgtta
     3181 aatcagactt accattcttt aaacttgtat tgttataaag gaaattttta ttgtcttctg
     3241 caaatctaat atattcctca acatactcac taccaaaaaa actattacat ttactacata
     3301 acgtattgga atggcaacag tttttttgac aaattttata aggtgcagaa cttctttccg
     3361 tatgctattc cgattgtcct tgacaatgag cctcctcgga tgtgacgatc ttcggcagga
     3421 gctaacagtt aaagttagac tgcctcgcta gcgttagcac atccgagctc attatcaagg
     3481 attcgctgcg ccaat
//
LOCUS       DAJSBN010000130         3393 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJSBN010000130 DAJSBN010000000
VERSION     DAJSBN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.134"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            577..909
                     /locus_tag="ONT86_002838"
                     /pseudo
     CDS             577..909
                     /locus_tag="ONT86_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            923..1072
                     /locus_tag="ONT86_002839"
     CDS             923..1072
                     /locus_tag="ONT86_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353567.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1069..1515
                     /locus_tag="ONT86_002840"
     CDS             1069..1515
                     /locus_tag="ONT86_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353568.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="ONT86_002841"
     CDS             1517..1654
                     /locus_tag="ONT86_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR2353569.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="ONT86_002842"
     CDS             1691..1984
                     /locus_tag="ONT86_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353570.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="ONT86_002843"
     CDS             1981..2205
                     /locus_tag="ONT86_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR2353571.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..3221
                     /locus_tag="ONT86_002844"
     CDS             2202..3221
                     /locus_tag="ONT86_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCR2353572.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT         1181 a          601 c          704 g          907 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gaccggatgt aaatcaagct tataatactt
     3061 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     3121 atgtcatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
     3181 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     3241 agcattacat aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg
     3301 catatcttca ctatcaccca taatagtcac actccaagct atgcgataac aggtttgttg
     3361 ccacacattc tactggttga ttgtttatgg ctt
//
LOCUS       DAJSBN010000131         3391 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJSBN010000131 DAJSBN010000000
VERSION     DAJSBN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.135"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(22..2493)
                     /locus_tag="ONT86_002845"
     CDS             complement(22..2493)
                     /locus_tag="ONT86_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2353573.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            2766..>3391
                     /locus_tag="ONT86_002846"
     CDS             2766..>3391
                     /locus_tag="ONT86_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2353574.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          999 a          686 c          609 g         1097 t
ORIGIN      
        1 tcttttttga gatacttttc gtcaattttc tcgtttaaat atcgatagaa attttttagc
       61 tacgcgacta aacggctttt gctctttttc atttttacgt tcaaacaaat attctaccaa
      121 tgtggtcaaa tgcctaaaaa tcgaatctgc cgagaaaaat agaatgctca acagcaaaag
      181 tgatacagct gagataccgg tgattgaaaa cagcttatgc aaaaagatga ccgaaaaaaa
      241 gttaccgcta gcacagaaaa gaatgatctg acttctgaat ttattcagcg gtgaacagat
      301 tttatagaga acgaggaacc ctactgcaat caaaagcatg gtagatgctg tagaaacatc
      361 tgttttattt agattcaaag tcaccccgca gatcaccaac gcaccgacta cgatcatatc
      421 tgttagtcca cctggcacag ctttttccat cacattcatg atgaatttcc ctctgattcg
      481 ctttttgttt tcttctagag caagcaagaa agatggaagt ccgatagtaa acaaactaat
      541 caatgtgatt tgtgatggtt ctaaaggata ggtcagagca aagatcactg aaaacagtga
      601 gagcaaaaag gagaaaatat tcttcactaa aaacaagctg gctgatcgct caatattgtt
      661 cacaacgcgc cgtccttctg caacaacttc tggcatgcgt gaaaagtcag aatcaagtaa
      721 gaccacttgt gaggcctgca tggcggcttc atttccggaa gccatcgcta tactgcagtc
      781 tgcttctttc atggctagaa tatcattcac gccatcacct gtcatagcta ctgtattgtt
      841 acgctttatc aattgaacga agattttctt ttgttctggc ttgacgcgtc caaaaactgt
      901 atatttttcg acagctgctt catattcttc ttctgataat ttagatgcat caacgtactg
      961 atcagcatgt gaaatccctg cttggacagc tacattggat acggtgagcg gattatctcc
     1021 agaaatcact ttgatggcta cttgctgttt tttgaagtaa tcaaatgtgg ctcttgcttc
     1081 ttttctgata ggattagcaa tcagaatata gcctaatggt gtgactgctt ctgtcaatac
     1141 tggttcttca agaatcccat cgtacacacc aaagaccaac acgcgatagc ctttttcagc
     1201 gaaagaaaca aactcttccg catattccgg caattgatcg cgcaaaacca tttcgggcgc
     1261 tcctaaaaca tacaccttgt ctgccaacgc tacactgctg aatttttgga cagaagaaaa
     1321 aggaataaca tggtgatagg attgatcagt ggtttttgta aaatatgctt taacagcttc
     1381 cattgttgca ttgtctcctg ccatcgcttt tgcatagtca gcaatgcatg cttccaattg
     1441 atctgactga ctgattattt cttgtttttt agagacgatc attttttgaa cagacatttt
     1501 attttctgtg atcgttcctg ttttatctac acaaaggaca tttacccttg ccaatgtctc
     1561 aatgcttttc atattgtgta aaagaactct ttgctttgct agtttagtcg cacttaatgc
     1621 taatgcaatc gtcgtcagca agtacagtcc ttcaggtatc ataccgatca atgctgcttc
     1681 cattgcaacg acactttctt tgatcgtgtt tccattaaaa aagtaacttt gagaaaaaag
     1741 caggataccg ataggaataa tgatgatccc aatccatttg atcaattgat taagagaagc
     1801 aatcatctcc gactgttctc cttctcccat tgtcttggct ttgactgtca gctgggaaat
     1861 atacgaatct gctccaactt tgtctaatcg ggcgtaagct tttccggaaa caatgaaact
     1921 gccggacatt aatggatcac ctatttcttt ggttatttca tctgcttcgc ctgtcaataa
     1981 agcctcatta acttgcagtt tcccttccac gacctctgca tctgctggaa tttgatatcc
     2041 agtctctaaa ataacaatgt catctattac aaggtcttct atatctattg tttgttctat
     2101 cccgtctcgt atgacattga cttttgctgt atttaaaaca tttagttcgt ccaagatctt
     2161 tttggatcga atttcctgaa aaatggcgat tcctgtattg gctatgataa ctggtaaaaa
     2221 agtcaagttc ttataggatt ctactaaaca aagcaaaatg gctaagataa gaaagatcaa
     2281 attgaaatac gtaaatatgt tttccaatac gatttgctga tttgttttga atgtgggatc
     2341 gactgaatta tttgctgcac ctttttctat tcgttccttg acttgatctg ccgttaatcc
     2401 ttctttatac gatggaaaaa gacggatgac tttatctgat gatctttgtg ttactttctt
     2461 ttttttactt cgatttatcc atttattctt catgtggcta cctcgattga tttcgttttc
     2521 tggatatttc caaacttctc ctatccagta tagaacagat atggaaagtg ttcctcacat
     2581 taagcttgca aacacctttc attcctgtca tatagcaaaa aaaaagggtt ctataatatt
     2641 ttgtgttggt ggaaaatcga aggcgatttt ctactaacac tttttcttcg tttcaactca
     2701 aaaagagact cacttttggt aaaatttagt cgaccaaaac gtcatcctac caaaggagtc
     2761 tctcaatgga taatcatact agaaaattac tcggattaac agataaacat ttattttttg
     2821 atgaagaatg gctgattgaa aaagaatata aaggcgttca agcgcagttt attaaaggaa
     2881 aattggatga ctcttcttcc tattgtgaac actgtcgatc ggttcgtatt atccgcaacg
     2941 gttcctatac gacacgcact cagatgttga aagtcaaaga aaagttaact attttagaat
     3001 taaaacgtac ccgttttctc tgccatgatt gtggaaggac cttttctgcg aaaacggact
     3061 tagttgatga acatcatcaa ctaacaaagg aactgaagca ggtgattctc atggaactgt
     3121 acgaaaatca atcacgtaaa ctaatcgcta agaagtactt cgtctcggat gggacggtga
     3181 cacggatctt gcgtgaagca acgaagcatt accagccacg tatgaacttc cttccgacgg
     3241 tgttgtgtat ggatgagttc aaatcaatga attctgtttc tggttccatg agttttattt
     3301 gtgttgatgg gacaaccaat cagctattta cgatccttga agatcgcaga ctttacaaac
     3361 taactcaata cttcctgcgc tttcctagaa a
//
LOCUS       DAJSBN010000132         3303 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJSBN010000132 DAJSBN010000000
VERSION     DAJSBN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.136"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1097)
                     /locus_tag="ONT86_002847"
     CDS             complement(<1..1097)
                     /locus_tag="ONT86_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2353575.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="ONT86_002848"
     CDS             complement(1227..2603)
                     /locus_tag="ONT86_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2353576.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="ONT86_002849"
     CDS             complement(2603..3259)
                     /locus_tag="ONT86_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2353577.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
BASE COUNT         1002 a          620 c          446 g         1235 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tct
//
LOCUS       DAJSBN010000133         3143 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJSBN010000133 DAJSBN010000000
VERSION     DAJSBN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.137"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(360..974)
                     /locus_tag="ONT86_002850"
     CDS             complement(360..974)
                     /locus_tag="ONT86_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2353578.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1580..1957)
                     /locus_tag="ONT86_002851"
     CDS             complement(1580..1957)
                     /locus_tag="ONT86_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353579.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2257..3138)
                     /gene="galU"
                     /locus_tag="ONT86_002852"
     CDS             complement(2257..3138)
                     /gene="galU"
                     /locus_tag="ONT86_002852"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR2353580.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          954 a          652 c          487 g         1050 t
ORIGIN      
        1 gataacaaat gtactataat tactttgtaa agagttttgg ggaagactct ttcatcaacg
       61 tgtattaaga gacaagaaag aatccatatg gattctttct tgtctctttt tccactactt
      121 tttatttttc tgacactaca tattcttata actcatttct cataactgtg aaacaactta
      181 taaaaataac aaactttaaa caaaacaagc ttaaaatcaa agatgatcca tcttaaaata
      241 actaaaacaa aaaaagtagt cgttgttaat cctcattaat aatcgttttc tttttttcaa
      301 acgctccatt tttatgtgga acattatttt tttaaggaac tttgtcaaca tgtggataac
      361 tattttccac ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac ttgctgcagc
      421 aaaaagatcg ttgcaagaga ggctgttccg cagatcgctg gatctagatt tggcaaagtc
      481 aagactgcat catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa ttcaaataat
      541 cgcaaaccac tgctatgttg cgatttgata cttcgtgaca aatcaaatcc agtaacaatg
      601 attaatggat gaccattttc tttgatccac tgagctagtt cgatgattgc cgggtttctt
      661 ccttcattcg aaagaagaag aaaaatgtct tctgatttga cagaaagctg atcaataatt
      721 gcctgtcctg caccttctag cgtttcatag acacctccgg ctggattttc aatcaatata
      781 cttggtacaa gatccggggt ttgatcccaa aattcaaatg ctacaccact tagtgtgcgg
      841 ctagcatata tctgcaatct accaccttga ttaatttgtt tcgcgatcca agtgccagct
      901 tgaagcatca gcggctcttc tatccttaat ttctcttcca attggtgata atagtcaaaa
      961 taacataaac tcatttatct ctcccgccta gctatacttg acatgaatag aaacagctgc
     1021 ctaatctaag gaagtacata aaaaaacagt agttagacag taacacatgt ggtctttgct
     1081 gtttcaaaca caaaaaaggc attgaacgaa tacgcccaat accttgacat agcaaataaa
     1141 acaacgtgca gaccatggaa aatatctaca taggggtaga attttcaccc tagattaggt
     1201 acaataaatt aggtcgatgc actgcattga ctatggcaaa atgatcggtc agggtgtatg
     1261 tgtgtgactg gtcattccct gattcttagc gccaactaag ggtcagggag ccttttttta
     1321 ttcagctgtt tctatctgaa tttattgtaa gtaatctctt atccaagtgc aagcaagaaa
     1381 atacaaaaaa acattggtat gatttatata tatataaata acataaaaaa aggaatggct
     1441 tatcccgata caacaagcat tcctttaaaa aagattcttt tatataagtg aaaaaagctt
     1501 ttttccacca attaaataaa tctctcatct caatagttta tccattattt tatctttttt
     1561 tgcgtatacc tactaaatat taccgtcttc ttctcgttct tctactttgt tcgagagaat
     1621 ctgtactaga aaaagtagtt gtatgtctac cttttctgcc tatccgagac tttcttttca
     1681 tatatgggat ataaatgatt agatacagta cagcaaaaaa taaagcaaat acgcaagctt
     1741 cccaactacc taacgccaat tcaaccatca aggtagcaac cccaacggat gctgcatacc
     1801 agataaaaat cagaaacgtt tctttttttc tgaccatgcg aaacatgata aatagactaa
     1861 taagcaatag tggcaacaca aatatccaat tgcttccttc catcagccac ttgagtatat
     1921 caagcagcga ctgtgcaaca gcgactaatt ttcccatagt gaacacacac ctttttttat
     1981 aattaaaaaa ttcgcctttt tattctacta tgaatcttct ggcttggctt tcatttctct
     2041 tatgtaatca ttttctttta tacaacttaa aaaaaaatta aaccattagc ataatatttt
     2101 ctgaatctat tcacgaaagt ctttttctat ctttcgtttt ccacgactca actgatctag
     2161 agtcaaaaaa ctaaaacgac agttgcgcaa atgacgcaca agctgccgct ttaataacaa
     2221 atagcctttc attctcagca ggccacttat ttccagttaa ctttctgttc ttagcttttt
     2281 actcaactgg ataatatatg cacttaatga agtcccaatt tccgaatgtt gaagtccata
     2341 ttcaatattt gtcttcatat atcctaactt atcgcctacg tcgtatcttt ttcctttaaa
     2401 ctctctcgca tagaccgcct ctgtctggtt aagagtctga agtgcatccg ttagctgtat
     2461 ctcgtttcct gctcctggtt cttgagtttc taatatctca aatattttag gagtcagtag
     2521 ataacgtccg ataatggcta aattacttgg tgcttttccc atttcaggtt tttctacaaa
     2581 cccttttact ctgcaacgac ccttttcgct atctgtttta ggatcaataa ttccgtattt
     2641 agatgtttca ttttctggta cacgcatgac ggcaacagcg gaagcttgtg tttcttcata
     2701 atccatcatc aattgttgag tcaatgggat atcatctttc atcaaatcat cccccagcat
     2761 gacgacaaac ggttcatttc ctataaatgc ttttgcttga agcacggcat cccccagtcc
     2821 tcttggatga gattgacgga tgaagtgaag attgacttga gtcgtttctt caactaaagc
     2881 gagtaattct gttttttcct tttcccgcaa attgttttcc aattcgatat ttgcatcaaa
     2941 gtgatcttca atgggtcttt tttcttttcc cgttacgatc aagatgtctt cgattcccga
     3001 atccaatgct tcttccacaa taaactggat cgttggttta tccacgattg gaagcatttc
     3061 ttttgccatg gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg caggaatgac
     3121 tgcttttttg accttcactt gct
//
LOCUS       DAJSBN010000134         3128 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJSBN010000134 DAJSBN010000000
VERSION     DAJSBN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.138"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            103..1347
                     /locus_tag="ONT86_002853"
                     /pseudo
     CDS             103..1347
                     /locus_tag="ONT86_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="ONT86_002854"
     CDS             1360..1992
                     /locus_tag="ONT86_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2353581.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="ONT86_002855"
     CDS             2197..2424
                     /locus_tag="ONT86_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR2353582.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="ONT86_002856"
     CDS             2493..2759
                     /locus_tag="ONT86_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR2353583.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="ONT86_002857"
     CDS             2749..3018
                     /locus_tag="ONT86_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR2353584.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DAJSBN010000135         3087 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJSBN010000135 DAJSBN010000000
VERSION     DAJSBN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.140"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(25..162)
                     /locus_tag="ONT86_002858"
     CDS             complement(25..162)
                     /locus_tag="ONT86_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR2353585.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="ONT86_002859"
     CDS             complement(159..1343)
                     /locus_tag="ONT86_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2353586.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1602..1892)
                     /locus_tag="ONT86_002860"
     CDS             complement(1602..1892)
                     /locus_tag="ONT86_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353587.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="ONT86_002861"
                     /pseudo
     CDS             complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="ONT86_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT          842 a          739 c          540 g          966 t
ORIGIN      
        1 aaattttgtc cgaaattcat cttgtcactt tcctttcttt tccattgctc gatccgctgc
       61 tgcacttgca ttcatcaagc acatcacagt aacaccaagc atactgccac caaataccaa
      121 aaaaatatct tttacaattc ccatgacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatcttttta tgtcggtctg ataagcgtgc attctgtacc
      241 atatcctgga tataggttgt ccgattcaat cgatccaact ccttagccat tttccacgta
      301 ggggcgactt gtcggcctag ccaattcaag gttcgttcaa tggtatacgg ttttggctcg
      361 gaagttaatt gaatctcttt ccgattccgt ccaataaaac gcccccattg ttcattcaag
      421 ggccagttgg ttcgcctttt accttctact ttatccgcaa atcgcaagta acgattgata
      481 atggaaaacg tggtgctctc aatgctacga ccttttaaca aatcttgaat cgcatgaaaa
      541 gcacggtcat ttttcagtcg aatctcaaaa cgatttttcg tctcagtttg gtctaatgga
      601 atccctaact tgatatattg ttcatagtct ttttcataca aacagaaata aacctcactc
      661 ttgagactgc cgatatataa ggtattgccc atgccgtcct tttcatcagc ctttaaaagt
      721 tccccggaac gataactttt aaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta gctcaggtat atccaatagc cccactcgat cattgattgc caagtccaag
      841 cgtttcatca cgccaccggc ctttagacaa tcctcgaaaa agtcgtacca gctacgcttt
      901 tgagcgagta aaaaagtttc aaattgtcgg caacctcgcc cctttaattc taaaagcaca
      961 cctttatctt cttcatgaga caacatcacc acaatatcac ccatgacata ctgttcttga
     1021 taggagtaaa aggcataatc ttcatggagc atatactcaa atttcaaatg gagaatttct
     1081 tgaataattt ttcgtgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gtcaaaggca aatcgggatt aaagctttcc agttggtcaa aaattttcct catggttttt
     1201 atagatgcgg ttacgccacc attttctatc tgatttaagt acggccgact aagtccacaa
     1261 gccgtagcca atttattttg tgaaacgccg tattcgttgc gtcggtcttt taatagccga
     1321 taatctaaat tccgttgtgc caagttctca cctccaaatt caaggtgtta actttttcaa
     1381 atgagttaac acctatacaa attacttact atctttgata caacaagcat tcttaatggt
     1441 tgcttactta ttttttgtgt ttttgtaccc ccctgttaga tacgggggtg agacatcatg
     1501 gtcggctaac gccgtccatg aacaggcaca gcaccacgga ggatttctgc ttcgcgaatc
     1561 ctccgcggtc agcgcctaac agtcagttgt gttggtttag gttactcatc tgtaatacca
     1621 tttacttcca actctgcttt catttctaac aagtattgac gaatatctgg ttcttttgct
     1681 tggtccgcaa tccgctcaaa ttctgcaatt acttgattta tttcaattcg agcatccgtc
     1741 tcctcattca ttaaatatgg atcgccagcg actttgacac cattcttgcg aatatcaaat
     1801 tttaaccgtc gcaattgttc ttctaattct gggtctgact gaatctttcc aatcacccaa
     1861 taaatttctc caatcaactc atcataaacc atgtacatcc aactctttta atcttctggc
     1921 aatatcaaaa atgaccggaa ctgttacaga gtttccggct tgcttataca actgactatc
     1981 tgaattgact tgagaatctc gatcaaaagc ccaatcggga aagccctgaa gtcgccaaca
     2041 ctctctaggt gttagcttcc gaatttgata ctgatccgtg acgatcgctt gttggtctcc
     2101 tgttagtaag gtatgaacac tctgttttcc cagacgacct cggcgacttg tggaattagg
     2161 aaaagccaga ttcacactgt cttctggctt cacctcttga tattctgttt cattttttcg
     2221 aatcatgatt tttgtaccac cttgtttcgt tgacaaggtg ggggcggttc cagtaacttc
     2281 ataaactcga ttaccttgtt caaactttcc cggtaatagt cctgctacag agattccatc
     2341 ccttgctggc agtgcgattt tgattccttc atcacctgtt aaggttggcg ctacgccatc
     2401 tgaaccatag acttgtccac ccattcctcg attggaagga ttgatattta ccaaatttct
     2461 gatgttttga tcagatgttg aagacggtcc aatgatagga aatacttgtt cggtacatgc
     2521 gtctcgagaa tgtccgataa ggaatatccg ctcccggctt tggggaatgt aggcctttga
     2581 gttaagcacc taccactcca cgtcataccc caatccatcc agcgttcgga ggatcgtctc
     2641 gaacgtagcc cctccttcgt gattgagcaa tcctcggacg ttctcaagga ataaaagctt
     2701 aggtcggaga agagtggtga accgagcgat ttcaaagaat aaagtacctc gagtatcgga
     2761 aaatctttgc cgctttcccg caattgaaaa agcttggcac ggaaatccgc cgcaaaggat
     2821 gtccactggt ccgagggatt gaataaactc atctgataca cttgtgatgt catgcatttc
     2881 cacctcctta cttgtatcat gaattgcttt ataactacgt ctagcaaatt tatcaatttc
     2941 acaaaaaccg atacaatgat ggctcgcctg ttccatacct aaacgaaagc caccaatacc
     3001 ggcgaataaa tctaaaaatc tcatacatta cctcattcat tgcaaagaaa aaagccaaga
     3061 acattttgct cttggctcat acattat
//
LOCUS       DAJSBN010000136         2893 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJSBN010000136 DAJSBN010000000
VERSION     DAJSBN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.141"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(260..544)
                     /locus_tag="ONT86_002862"
     CDS             complement(260..544)
                     /locus_tag="ONT86_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353588.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
     gene            complement(565..1935)
                     /locus_tag="ONT86_002863"
     CDS             complement(565..1935)
                     /locus_tag="ONT86_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HCR2353589.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            2154..>2893
                     /locus_tag="ONT86_002864"
     CDS             2154..>2893
                     /locus_tag="ONT86_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR2353590.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSQLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHSLLGKQRKTV"
BASE COUNT          912 a          590 c          439 g          952 t
ORIGIN      
        1 atgtgaaaat cgaatcttga gatagctatt ccccttgaga tattcactga tacttctact
       61 caaatcattt aaatttttat aattatttgt acggaacgta tatatatcaa catctattta
      121 gacgttgtcg gtgatacaat gtcatttttc ccgtgtacta tgtcatttat ctctatactt
      181 tgtcattttt ctttttgggc aatcaaaaaa ggcaacaaac caattatgat ttgctacctt
      241 ctccgtttaa aaacttatct tagccccaaa atggaatcca atttttaact tcttttgcca
      301 atgcatacgc cttagcaaga cctgagtttt caactaaata tttttcccct tccagagaaa
      361 cactccccca gatatgaaca gtgttatccg cccaaaccat ggattctagt aagccattgc
      421 gcgaaagaaa tttagtttgt tctaaaatat cttcttctgg aacttgccct ttaagctcaa
      481 taaaaggatt ctttttttca tcacgcaatt taagaattgc atatcttagt ttgttcttat
      541 ccaacctaac accacctcta tgctctattt cttatttctt agcgaatcat taatacttat
      601 tccgttatca tatttccagt agtcccaacc attccggcta ccccctaata gcagagctgc
      661 tgcattcgaa acagatgagg cagtgtaatc ttgaacaaat tgtagtcccc caattgtttc
      721 ttcaatgatt ccattatcca acagctcttg gcgagtttca tctagcgaat cagatgcaat
      781 atctttaatc ttagaataaa tttctgaacc tgaatgtata atcacggtct cgtcttcgtt
      841 caaataccca gttgctttta ttccttgcgc ctctaagaaa atttccctat tcccatcgtg
      901 ttcatagtcg cccgacgatc tcaaataggg atacatagta atcagttcat tgattagagc
      961 ttcacttctg gcagaaatat ccgatgatgt ccactcagtc aattcgtaca aatctttatt
     1021 catacgaata tgaagggtat cttcaaagat tttctttttg gtctcaaagt ctttatttcc
     1081 cgcggccgaa ttatcaggat ttgtaacgag tgttaaatta ccaatggtat ttactaatcc
     1141 tgtgtattct tcccccgatg taccagcctt ctcttcccaa tatgaagtac tagtctgtgg
     1201 catgatgtgt tcaatggaca agttactcat atcaagtgtt gctgaatttt ctcgattctc
     1261 tattttttca agtaaccaac gggccaatct catcgcataa gcattggcat tcaatagatt
     1321 agatttcaat gccttgtcag atggaagtgc catattattt gattgatttc tagtaacaag
     1381 aacataaatt acaatatcaa cgatattttc atatccatta gctttagcgt acttcctaac
     1441 acttttcaaa tatgaaggaa atgcttttga cactctgctt gtatcatccc cattaaaata
     1501 tctcctaagc tgataatcat tcactacctt aatcgcatca atatattggt cttcagtgat
     1561 aaattggtca tgatagtacc attcagataa tcccaaaaca aaaggtgcag gcatcatact
     1621 ttccatactt tggaaatcgc tcaatacttc tgagtatttt ccttcaggtt tgttatagta
     1681 aagtctcgca aaataaccag catatcgcac taatttttgg agcaattcgg catcacttga
     1741 tacatcgcgc tctttatgcc aatagtcttt gaatgcttga taaagaatat gctttgctga
     1801 atactcccca gttattgcag caaggtagta tctgaaaaag tcttccattc ctttagaatt
     1861 atcaaagact tcttccaacc tacgccaata tttagtataa agcgtagtct gctcttcatt
     1921 agatttatcc atcatgatga agtttcgaat caaatccgag gcagtcaatc tttcaccggt
     1981 agaattgata ctttcaaaaa tttgctgtgc attatcacgg tcatctaatt taatataaac
     2041 aatacgcgtg gtgctagtta attttttcct agaggaaaat gtctaaacat gttggtaaat
     2101 caacaaaaaa cgcatatagt agtctcaaaa ctcccaagaa atgaggacta catatgcgtt
     2161 catcaaaata tactgaacat tatacagata cttttataac attcaaggaa attatgagaa
     2221 ctgtttctaa catataccac aattgtgtac cagataagat taaaaaccga agaaatactg
     2281 atcaactgaa gcaacgcgat acagtgatta ttgcttgtgt tatatggggc ataattaatg
     2341 gctatactag ccaaagagcc acgtatagag cggtttgttc cgtcttattt cttaatggtg
     2401 actttcctag taggagtcga ttcactcgct taagttcaaa cttagcttac actatcaaga
     2461 ttattcgata ctttttcatc aagaagctca ccaaaggtga actagtcgga attatagata
     2521 gttttcctag tcaattatgt aaaccagtta gaaatagaca agcaaaacta ttgaatcaaa
     2581 tagccaaagt tggctataac tcaacaaaaa aatcttattt ttatggtctt aaaattcata
     2641 tgatcgttac taagactggc tttccgatta cttactcaat cacgaatcca ggtgtccatg
     2701 acgtgaaagt gttagagact ttatcggaag aagcgaatct tcccaatatc ctaggggaca
     2761 aaggatatat tagccataaa atccatgaaa agcttgccct taagggcatt actatatccg
     2821 ttccgccacg taaaaacatg gataaatcag aaaaactaga ccacagctta cttggaaaac
     2881 aaagaaaaac agt
//
LOCUS       DAJSBN010000137         2879 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJSBN010000137 DAJSBN010000000
VERSION     DAJSBN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.142"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(615..1232)
                     /locus_tag="ONT86_002865"
     CDS             complement(615..1232)
                     /locus_tag="ONT86_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353591.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            complement(1294..1974)
                     /locus_tag="ONT86_002866"
                     /pseudo
     CDS             complement(1294..1974)
                     /locus_tag="ONT86_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(2296..2517)
                     /locus_tag="ONT86_002867"
     CDS             complement(2296..2517)
                     /locus_tag="ONT86_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353592.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(2615..2860)
                     /locus_tag="ONT86_002868"
     CDS             complement(2615..2860)
                     /locus_tag="ONT86_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353593.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          888 a          522 c          412 g         1057 t
ORIGIN      
        1 tagttttttt tgtcacataa ttttgcaatc aataaatgtt gcgcaataag tataagaatt
       61 ccattttcta aatttgaaag gtgatccata atttcatgtt caagtagttt attaattgtc
      121 cacttgtcgc ttcatctaaa aattttatag gttttaggaa tgctattcgc ttattttagg
      181 aagattttaa ctgtttgcta tagatatata tcatactagt atcgtaataa aagtactcta
      241 aaagaagaat tttcaaatac gcaaaattaa gtacgaaaga gttgaaacaa atttggagaa
      301 aaatgagcat agaatgtctg atgagttaaa tagacctaaa aattatctaa aagtggagca
      361 aaattaggaa ttacaaacaa aacactaaat tagtttatat aattttccgt aatatttaat
      421 atatatgaaa aggaaaacgt agaaaattac gtctacgttt tcctatttag ttctatattt
      481 tcattaaatt ttatatttgc tcttttcact tttcggaata aattcactgt gactcttgat
      541 accttctttt atatattgct aatgtatttt ctccattgct atgaaaatag aatcaacata
      601 tttataatat ctatctaatc tattttcatt tcaccgcagc atcttattaa ataacgtgta
      661 aataagaata taaaagttat tgaactaatt aataaagata gaatcatcgt agttggatac
      721 gtagatgaaa cctcaactat agttcttttc cccgtacctt taatagacac taaaccattg
      781 tcattttcta tagtgctctt tttcccattt actttcacaa ctgttcccca ataattgaat
      841 acaggtaatg tgatattttg ctcatcggca tcttctactg tatagactat tttggaatta
      901 tatgctttat gagatgcatt ctcccaccga tcaccaactt ttacttcatg tttctttaaa
      961 tgatctttat atttcaatgc atctaatggt gcatagtcat ataggcctcc agttcttgag
     1021 gatgtataac gtgtaacatc ttttttggtc ataactaaac gacctttata cgactgaatt
     1081 ttatgcgaat aatttgcttc tgttgctgca gtcacagtaa aaataataag aactattcct
     1141 acaaggctcc acatactttt tttgttgctt gttttccact ttaaaaacac tatggttaag
     1201 attaatgaac caaaaagcac ttgaaaaccc aaggttctcg gttctgttgc gaagtttgaa
     1261 aatcaaacgc gccctctctg aactccaaat atcttaggct gttattccca ttaatacctt
     1321 gatttcagta gacaccgaaa agccgaagag cgttccattt cttcggttct ttttatatat
     1381 tcctcgaatg gtctccatgc ccttaatcgt ggaagaggct gtacggagac tttgataaaa
     1441 tttatttcgt cgtttaatag gtcgatggtc ttgttctatt aaattgttaa gatacttcac
     1501 agttcggtgc tctgtcttag tatataaacc cacactctgt aactttctaa aggcggagcc
     1561 aagagaagtt gctttatcgg tcacaattgc tttcggctca ccaaactgtt tatggagtcg
     1621 ttttaagaaa gcataggctg cttgcgtatc ccgtttcttt cgtaaccaga tatctaaggt
     1681 taagccgtcc gcatcaattg cacgataaag ataatgccaa cgtcccttaa ttttgatata
     1741 ggtttcgtcc attttccatg aatagaagga ttgtctattt ttcttcttcc aaagataata
     1801 gaggactttg ctgtactctt atacccaacg ataaatcgta gtatgacaaa catttattcc
     1861 acgatcatat aacaattcct gaacttcacg atagcttaga ttgtaacgca ggtagtaacc
     1921 aacagcgaca ataatgacgt cttttttgaa ttgtttgcct ttaaaatgat tcattactct
     1981 gtcctctctg tcttttttct caattttaca ctaaaataga ttttttggaa aactttgcaa
     2041 cagaaccgtt tttactatat acttcttccc agaacttgtc cgtctccttc aaattatctc
     2101 tacctctcga gttcattggg tggtttgccc atttattaaa catggaatca cctttctcat
     2161 actcatatat ttatgtccta tttcaattat atttatatat gcttaaataa atttgtatcc
     2221 tgaaatttat tgtaatatga agctgtattt tacatatagt aaccaaagag ttaataaaat
     2281 aaaaagagcg aaccattata gatcagctct tttttcattg ctgtttttaa tcttatctag
     2341 aatcacttca aatgtttcaa aggtatttac atctaactga cataaccaaa gcaagtactt
     2401 cttttctttg taagtccaat caatattacg gtaagaggac attagattat ggtaccttgc
     2461 tctttgttca actgaagcca attcccagcg tttttcaaat attttttctt cttccattat
     2521 tgatccctcg caagacgcta cctcaaactt aaataatggg cagcgaaagt ctaattcctt
     2581 agatctaagt atagcaaaca aatatgaaaa aaacttagaa ggtagtaaaa gttcttccgt
     2641 cttttgcttt gcttctattc ttaatgtgtt tcgttatcgg tgccctacaa agattttcat
     2701 taattacaca ctcatttatt gttgtgtatt ttctgacatt tctctcttga tcaattattt
     2761 taattccatg ggcatcctga tagttcatag gagaggagcg gctatcagaa atagttgcac
     2821 ctatctcttg aacttctttt acaacttctg gaaaatccat ccatcctgct tcagctgtc
//
LOCUS       DAJSBN010000138         2844 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJSBN010000138 DAJSBN010000000
VERSION     DAJSBN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.143"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            144..494
                     /locus_tag="ONT86_002869"
     CDS             144..494
                     /locus_tag="ONT86_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2353594.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="ONT86_002870"
     CDS             908..1726
                     /locus_tag="ONT86_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2353595.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1799..2158)
                     /locus_tag="ONT86_002871"
     CDS             complement(1799..2158)
                     /locus_tag="ONT86_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2353596.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          969 a          399 c          505 g          971 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt
     1801 acgaagcgtc tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat
     1861 tctttaaacg aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa
     1921 aggtagtatc gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat
     1981 atgctggaat ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc
     2041 cctctgctga agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta
     2101 aacgaggtac ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt
     2161 tcacgaccac catcgacttc cacagttgaa attgacattt tatttaacac acctattccc
     2221 ccttaactat aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc
     2281 aaatcgccaa tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg
     2341 taaaaattaa accgaagatc tgataaattt tctctatttt ctacaatatt taaaacgctt
     2401 ccttagtcta ttatatatct atttctctta ggagtatgtt tagatgtttg aataatagtt
     2461 gcccaacttt aaaggttatt cttataatat tttacttcat tttccaatag aaaattcaaa
     2521 ggattactgg tttttctaaa atagtttagt ttaaatacat aagtatcctc cgacactgta
     2581 ttagttcata atcatctaaa tacaaaattt tcaaataaaa atatttcaag ctctatcgtt
     2641 gacgaatgta aacgatactt ataaagtaat agaaattgat tacatttgta aacttaaaat
     2701 ggttaattta tctcacataa aactaagaat aaaataatga ttaaaagtgg ttagttttta
     2761 tacgatttag cctatcggta aaaatgaatt agagagtgta aaatattttg tgtaaatgaa
     2821 aaaatccata caaaaaagga agtc
//
LOCUS       DAJSBN010000139         2826 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJSBN010000139 DAJSBN010000000
VERSION     DAJSBN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.144"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            798..1052
                     /locus_tag="ONT86_002872"
                     /pseudo
     CDS             798..1052
                     /locus_tag="ONT86_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="ONT86_002873"
     CDS             1122..1634
                     /locus_tag="ONT86_002873"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2353597.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="ONT86_002874"
     CDS             1631..2668
                     /locus_tag="ONT86_002874"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2353598.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1022 a          496 c          564 g          744 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacac
//
LOCUS       DAJSBN010000140         2820 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJSBN010000140 DAJSBN010000000
VERSION     DAJSBN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.145"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(61..936)
                     /locus_tag="ONT86_002875"
     CDS             complement(61..936)
                     /locus_tag="ONT86_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2353599.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(1191..1275)
                     /locus_tag="ONT86_002876"
     tRNA            complement(1191..1275)
                     /locus_tag="ONT86_002876"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1239..1241),aa:Leu,seq:caa)
     gene            complement(1293..1364)
                     /locus_tag="ONT86_002877"
     tRNA            complement(1293..1364)
                     /locus_tag="ONT86_002877"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1330..1332),aa:Gln,seq:ttg)
     gene            complement(1374..1446)
                     /locus_tag="ONT86_002878"
     tRNA            complement(1374..1446)
                     /locus_tag="ONT86_002878"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1411..1413),aa:His,seq:gtg)
     gene            complement(1508..1592)
                     /locus_tag="ONT86_002879"
     tRNA            complement(1508..1592)
                     /locus_tag="ONT86_002879"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1556..1558),aa:Leu,seq:caa)
     gene            complement(1613..1683)
                     /locus_tag="ONT86_002880"
     tRNA            complement(1613..1683)
                     /locus_tag="ONT86_002880"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1649..1651),aa:Cys,seq:gca)
     gene            complement(1729..1800)
                     /locus_tag="ONT86_002881"
     tRNA            complement(1729..1800)
                     /locus_tag="ONT86_002881"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1766..1768),aa:Gln,seq:ttg)
     gene            complement(1812..1884)
                     /locus_tag="ONT86_002882"
     tRNA            complement(1812..1884)
                     /locus_tag="ONT86_002882"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1849..1851),aa:His,seq:gtg)
     gene            complement(1887..1960)
                     /locus_tag="ONT86_002883"
     tRNA            complement(1887..1960)
                     /locus_tag="ONT86_002883"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1926..1928),aa:Trp,seq:cca)
     gene            complement(1967..2047)
                     /locus_tag="ONT86_002884"
     tRNA            complement(1967..2047)
                     /locus_tag="ONT86_002884"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2011..2013),aa:Tyr,seq:gta)
     gene            complement(2049..2124)
                     /locus_tag="ONT86_002885"
     tRNA            complement(2049..2124)
                     /locus_tag="ONT86_002885"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2089..2091),aa:Phe,seq:gaa)
     gene            complement(2160..2232)
                     /locus_tag="ONT86_002886"
     tRNA            complement(2160..2232)
                     /locus_tag="ONT86_002886"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2197..2199),aa:Asp,seq:gtc)
     gene            complement(2242..2314)
                     /locus_tag="ONT86_002887"
     tRNA            complement(2242..2314)
                     /locus_tag="ONT86_002887"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2279..2281),aa:Val,seq:tac)
     gene            complement(2330..2401)
                     /locus_tag="ONT86_002888"
     tRNA            complement(2330..2401)
                     /locus_tag="ONT86_002888"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2366..2368),aa:Glu,seq:ttc)
     gene            complement(2416..2505)
                     /locus_tag="ONT86_002889"
     tRNA            complement(2416..2505)
                     /locus_tag="ONT86_002889"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2469..2471),aa:Ser,seq:gga)
     gene            complement(2545..2618)
                     /locus_tag="ONT86_002890"
     tRNA            complement(2545..2618)
                     /locus_tag="ONT86_002890"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2582..2584),aa:Asn,seq:gtt)
     gene            complement(2628..2743)
                     /gene="rrf"
                     /locus_tag="ONT86_002891"
     rRNA            complement(2628..2743)
                     /gene="rrf"
                     /locus_tag="ONT86_002891"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          829 a          652 c          536 g          803 t
ORIGIN      
        1 aatcatagtc catttactat gtgtcatttg gttgcgccta tagatagtta cgcccttatt
       61 ttaatagatt cttgctagat gaattgccaa atagatgatt tcattttctt gaatggcaag
      121 ttgaaattgc tgttcgatga tatctttaat ctttagtgct gtacaatacg ctttgctatg
      181 tttaaatgtt tcagtaaaaa aaccaatatt ttctactggc gtttcttcct gataaatcaa
      241 gcgtttaacc aggaatttga tatgaaccat taagcgatga taagtcagtg attctttatc
      301 ataaacgttt gaataggatt tttctagatg ggacaaaatc gtggtcgtca tttctactat
      361 ttttaagctc tttaaactat taggctctcc tagctcagca ttaatgatat gcatagtgat
      421 gaatcctact tcatttgacc ctaactcggt ttgatatcga tgattgattc gctcgaccac
      481 ttctactgct atatcatatt catttggata aaggaatctc atttctggca acataattgc
      541 tgtgacatct ctatcttctc tttttcgttg aatagcaaaa taaatatgat ctgtcaatgt
      601 aatatagata tttgaatcta atggagagga gatcctagct gttatcagtt caatcacttc
      661 ttccgcaaaa tccataaatt cgattggaat ctgcgagact atctcactga atcgtttgat
      721 cttttcttta gaatctaata caaataattt gtcagcatcc tcaacatcaa atggcttact
      781 cactttagca gaaaagccaa tccctttccc catcgcaatt ttttctattc catcgtcatc
      841 gatcaataca gcattatgat tcagcttttt catcacatac accgttaatt cacctcctct
      901 atatgataaa acaaaacaac ttgcagactc cttcatacat atttttgtct ccaaacaatt
      961 tttaaagaca aaaaacgacc taagaaagaa ccctttctta ggtcgtgcct aattgaatag
     1021 tagcacccta cataatttac aaaaatagtt taacacataa taaaagcgtt atcaatatct
     1081 tttttactcg ttttatgata atacttgtat cgaattgatt tggaggtaat tacataaaaa
     1141 caaaaaaaat atagcaactc tctataagaa agttgctata ttgataatta taccggcggc
     1201 cggggtcgaa ccggcacgtc ctcaacggac actggatttt gagtccagcg cgtctgccaa
     1261 ttccgccacg ccggcaaatt taaaaataat aactggggta gctggattcg aaccaacgca
     1321 tgagggagtc aaagtccctt gccttaccgc ttggctatac cccaagaata aagggcgaat
     1381 gatgggaatc gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac
     1441 attcgccatc ataaaagatg ttatttaatt tctcaataaa aaactaagat gaagacatct
     1501 tagtcaatac cggcggccgg ggtcgaaccg gcacgccctc aacgggcact ggattttgag
     1561 tccagcgcgt ctgccaattc cgccacgccg gcaagttgat ttattagtac taaggcggta
     1621 accggatttg aaccggtgat gaaggttttg cagacctctg ccttaccact tggctatacc
     1681 gccattatac tattatcaat ctataaaata aagaataaat tatagaaact ggggtagctg
     1741 gattcgaacc aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca
     1801 agaataaaaa gggcgaatga tgggaatcga acccacgaat gccggagcca caatccggtg
     1861 cgttaaccac ttcgccacat tcgccatggc aggggcagca ggaattgaac ccacactaac
     1921 ggttttggag accgctgttc tacctttaaa ctatgcccct ataaaatgga ggagagtgga
     1981 ttcgaaccac tgaaccctaa ggaacggatt tacagtccgt cgcgtttagc cacttcgcta
     2041 ctcctccatg gtggcgcggg acagaatcga actgccgaca cacggagctt caatccgttg
     2101 ctctaccaac tgagctaccg cgccatattt taaaattatt aattgaataa caagaacgac
     2161 ggttccgacg ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta
     2221 caccacggaa ccagttcatt atggaggtta acgggatcga accgctgacc ctctgcttgt
     2281 aaggcagatg ctctcccagc tgagctaaac ctccaacatt caattaaaat gacccgtacg
     2341 ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg accaacgggc
     2401 caggtaaatt tgctacggag agtaagggat tcgaaccctt gagacagttt ggcaccatct
     2461 acatgatttc caatcatgct ccttcggcct ctcggacaac tctccagagt gaaagaagct
     2521 caatactctt tctcaaaatg agaactccgg cagtaggact cgaacctacg acatcatgat
     2581 taacagtcat gcgctactac caactgagct atgccggaat aacatcagcg tggcgacgtc
     2641 ctactctcac aaggggcaac ccctcactac aatcggcgct aagaagctta acttctgtgt
     2701 tcggcatggt tacaggtgta tccttctcgc tatcgccacc acactgtggt gttatctttt
     2761 attgagtaaa ttttgttcac tcaaaactgg atttgaagtc acataagttt ttttccgagt
//
LOCUS       DAJSBN010000141         2782 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJSBN010000141 DAJSBN010000000
VERSION     DAJSBN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.146"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1412
                     /locus_tag="ONT86_002892"
     CDS             <1..1412
                     /locus_tag="ONT86_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2353600.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>2782
                     /locus_tag="ONT86_002893"
     CDS             1546..>2782
                     /locus_tag="ONT86_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2353601.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQ"
BASE COUNT         1094 a          381 c          553 g          754 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aa
//
LOCUS       DAJSBN010000142         2704 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJSBN010000142 DAJSBN010000000
VERSION     DAJSBN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.147"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            123..515
                     /locus_tag="ONT86_002894"
     CDS             123..515
                     /locus_tag="ONT86_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353602.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(688..870)
                     /locus_tag="ONT86_002895"
                     /pseudo
     CDS             complement(688..870)
                     /locus_tag="ONT86_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            857..1054
                     /locus_tag="ONT86_002896"
     CDS             857..1054
                     /locus_tag="ONT86_002896"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353603.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1097..1672)
                     /locus_tag="ONT86_002897"
     CDS             complement(1097..1672)
                     /locus_tag="ONT86_002897"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR2353604.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1827..2353)
                     /locus_tag="ONT86_002898"
                     /pseudo
     CDS             complement(1827..2353)
                     /locus_tag="ONT86_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          864 a          456 c          424 g          960 t
ORIGIN      
        1 attttttctt ttaagtaaaa aatataggtg attttttttg acttcatatt tataataaag
       61 acacgtaaat caattatttt tcattttact ttaactaata atgtttattt ggagggatta
      121 ttatgttaga tgatagattg acgtgttcta attgtggtac attattttac tatgaggaag
      181 ttagaaatat tgtaaaacat agaaaaaaag aaatgcctat acgttgtcca aattgcaatg
      241 aagttattaa gcgaaagttg tctcatggtt attttgtcag ctacgaacta tatgaagaaa
      301 gtaaaataaa tctgaaaaaa aaaccaaaat actataaaaa tttgaaaact ggagctatta
      361 taactgaaaa agaatttaaa aatttagtga ctgaagaaga aagcaggttt tataatttgt
      421 ctggtaatat ttgccctgga tttatggatc agattattga aaatgagtca attgaatcaa
      481 ttgaaaacta tgttcctgtt gacttaccaa aatagaaaaa ataatcctgt ttttacagtg
      541 aattatttta attttgattt tcaataaaaa tatattacaa taataatcgt aatagctaat
      601 gaaacaattt ctcaatcttt ttaccactcc aaacagggag cgtcaataat tttgtgtaaa
      661 taactcgtcc ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt
      721 cacttgttgg aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat
      781 ggatactagg aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta
      841 tttcttgatt tgtttattga aagattcgat tgtagaacca gaaggaatgg agaacacttt
      901 tgaactttac aacaaaacta ctgttgataa attaattcca attttagttc caactgattt
      961 aaatggtcat gatgttttac ttcgttttaa cggattttta ggtgaggatt atagatacct
     1021 aaaaatttct gatcctcaac ctttaacaga ataataaaaa acgtgtaatc aattatttga
     1081 ttacacgttt tttatttcaa tcttcgttgt agtagtggtt gcgacatacc gaaaggtaat
     1141 cttcattacc tcctactaca atctgttcac ctgaataaac tggaatacca tcaacaatac
     1201 gagcattcat tacagccttt ttattgcaaa aatggcaaat agttttaatt tcctcaattt
     1261 tatcagcaaa tgtcaataat gcttcggatc ccttgaagag attattttga aaatcattct
     1321 tcaatccata gcaaattact ggtacttcct cattcattac gataagtgat agatcaataa
     1381 tattctctct acttaaaaat tgagactcat cgaccaaaat acaatcaact tttccgtact
     1441 gatcaataat ctccactaat tctccgtaca catccatttc agaagtaatt gcaatagctg
     1501 gtttcgaaac acctatacga ctctcaactt ttccaaatcc tgatcgagta tctattgcgc
     1561 ttgtcattat gataactctc ttcccttgtt cttcataatt atgagccact ttcagtagct
     1621 caatactctt tccactattc attgttccat atctgtaaaa taattgtgcc attaaattta
     1681 ttcccttctt ttacaattca attattcctt aattccatct gcttttatgc agctgctcgt
     1741 ttattccttg atatgtctat aacatctact atccctaaaa ccaatttata agccattaga
     1801 gatggtattc ctgaacatct ctcatattgt tttttgagtg gtatatcatc tgaaaaactg
     1861 aaggaatcat gaaaatagcg acatacttat catttagagt tctaacttta aatgcattta
     1921 cttcttcagt cagcacttta gtcatgttgg acatggtttg gggcgtgtag tgatgcccat
     1981 acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa
     2041 tgacggtctc ctctaatgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga
     2101 actcgccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg
     2161 tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcgtactttt
     2221 cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt attgacagct ttttccaaat
     2281 ggacacggaa taatccattc aaatcgcctt tgttcgctag agtctttagg atttctgtag
     2341 taaaatcgtt catatggaag tcctcttttc tgtgaatgtg ttgtggtaac tctattctac
     2401 agaagggact tccttttttc tatttacaca aaatatttta cactctcaac aaaagtatct
     2461 tcgattaata cttgctcaag ttatgtgcag ctacgaaaaa gtaggtaccg aggtactttc
     2521 tttttcgcag ctttttttgg acttatgaga agtacattcg ctggaagaat acgatataaa
     2581 acgagctttt tcactaaaga tattaataga gtgaaagaaa caagcttttg ttgtcagttt
     2641 tttcactttt ctaaaaatga aaataaaaaa ttctggaagt ggagaagaat tttgcttgct
     2701 atac
//
LOCUS       DAJSBN010000143         2640 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJSBN010000143 DAJSBN010000000
VERSION     DAJSBN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.148"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            101..2542
                     /locus_tag="ONT86_002899"
     CDS             101..2542
                     /locus_tag="ONT86_002899"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCR2353605.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          882 a          464 c          617 g          677 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
//
LOCUS       DAJSBN010000144         2514 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJSBN010000144 DAJSBN010000000
VERSION     DAJSBN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.149"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..330)
                     /locus_tag="ONT86_002900"
     CDS             complement(<1..330)
                     /locus_tag="ONT86_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353606.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNL"
     gene            complement(1044..1334)
                     /locus_tag="ONT86_002901"
     CDS             complement(1044..1334)
                     /locus_tag="ONT86_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353607.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1542..2234)
                     /locus_tag="ONT86_002902"
     CDS             complement(1542..2234)
                     /locus_tag="ONT86_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR2353608.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          745 a          462 c          351 g          956 t
ORIGIN      
        1 taaattttta acatttattt tctcaaaaac atacttgcaa agtaatacta caattcctaa
       61 cataatcgta gaatttaata atttccatag cataaagtgt ttagaaaaga acattaagaa
      121 tagttctata ataattcttg aactccatgt cttataatgt tctatcgaag cacccaaata
      181 gccaagttca tctaatctac ttgcataaac tacgtcatcc gctctcatag tcggcatata
      241 catattaaag agtaaaaaga taccccaaat ccctattagt aacaagatca gttctttgtt
      301 attcgttatt tttctattat gtttgttcat ttttatactc cttcttatat ttcctaatag
      361 agtataacat agctttaagc attacttaaa gataacttaa aagttatctt ttaaatactt
      421 aattgttaaa taccttattt gatgcaaaag aaatctacga tagtcaacaa aaaaattttc
      481 attatatcca gaaaaatttt tcttagttaa ggacctatgg attgcagtaa tgcagaattc
      541 gtcgaataaa ctgccaaaga catacaatca tattgctttt aaaattgacg aacaagatat
      601 agattcgttt gtctcaaaaa tccaaatgtt aggtttaact gtcgaaccag gacgttctcg
      661 tgtgaaaggc gaggcttctt caatttactt ttacgactat gataatcacc tcttcgagtt
      721 acataccggt actctccagg aacgtttaaa atcctacaat tccactacct gagactagta
      781 atgactagtc tctttttatg gcctctgatc cccccattga gcgaatacta atagggcttc
      841 gaattgatct gttcaatctt taagcaatat ccctcaaatg agtttggaaa gtttagtgat
      901 tcatcacaaa tacttaaata catgtagtta tatatagatt gaggttcttg tgagcgtatg
      961 ctcgtgtggg ttcaaatccc atcgtctgca tccatctttc caaaacaagc ggctaggaca
     1021 tttttgtctc agccgcttgt tttctagttt attctttcat cttgtcggac cttttttctt
     1081 gggtatagac ttttctcata ttctaccaca attttgggtt tccctaacac atgataggat
     1141 ctcacaacaa attgctttct attattataa taacctgtaa cgactaactt catcccttcg
     1201 tctacttcag ctaggaagtt caaactatgg gaagcaatca aacaattgac cccatttaaa
     1261 ctgaaacgga tcaacggtct ggcactaact tgcaaagtac ggatcttatc tactgttcca
     1321 tattttttct ccataacctt cacctcaata ccattatacg aacatacatt ccctttgtaa
     1381 agaagaaact ctgttttatc tttttgactt tttgtctatt acctttctta aaatcatatt
     1441 ggcacttcct gcgaagatga aggacatgta ctaaaaaaaa gaggcagcag tatagattac
     1501 tgttgcttct ttactaattt aggtttactt gttaagaaag attatttacg caaatgttct
     1561 tcaacaattt gctgagattt atcagctgta tcttccactt catcttttgc tttttctaac
     1621 ttttgaatat attgtttcaa gcttgagttt tcagcagttg cttcgtctaa tttcttttgt
     1681 gtatctgtta actgagtttc tacatttgtt ttttcttgtg tcagtgttgc gatttgttga
     1741 ttcaaaccat ttacagtatt ttgtaattct gaaagttttg cttgtgcttc ttgtagtctt
     1801 gctgtccaat ctgcattcag atgattgatt tcatcatttt tttggttgat ctcatctagt
     1861 ttgacattga tttcattttg tttgctttgt agttgtgcat ccttgctgac taattgactt
     1921 tctaagtttt gtttttctgc tgccgattga tttttttggt tttctaattc atttactttt
     1981 gtttggtatt cttggacatc tgcctttgaa tcttgcaatg tctgttcttt ttgtgctagt
     2041 cgagcggtaa gatctgtaag cgcttgctct ttatctgtca atgtatgatc aagctgtgtc
     2101 agattctcat tgattgtatc aatatctgtc tctccacccc acaaaataat ttcatttgcg
     2161 taccgcgtgc ctgtgtagcc aattagacat agaaatgcaa cgacaatccc accagtaata
     2221 aatttctttt tcatttgcat agctccttaa tccaatactt ttacatgcta aaagtaagtg
     2281 atctcaaaca caatgtcaag cgtttttaaa ttacatgtgt aattttaata taaatactat
     2341 caaatactca tttaatcctc cttttttatc tatttaaaaa atctttttta tagagaaaaa
     2401 tgcaggttaa tactttctta aataaatatt tatggtgaac aaaaacaaaa aagcaagttt
     2461 tattaaacgt aacccttttc atttcataga ttactgtatt tattacaaaa aacg
//
LOCUS       DAJSBN010000145         2507 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJSBN010000145 DAJSBN010000000
VERSION     DAJSBN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.150"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1211)
                     /locus_tag="ONT86_002903"
     CDS             complement(<1..1211)
                     /locus_tag="ONT86_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR2353609.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKM"
     gene            complement(1201..>2507)
                     /locus_tag="ONT86_002904"
     CDS             complement(1201..>2507)
                     /locus_tag="ONT86_002904"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR2353610.1"
                     /translation="FNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSK
                     KLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTP
                     HMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTT
                     YNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFL
                     DDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLL
                     EDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEE
                     NIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDI
                     KKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT          697 a          528 c          418 g          864 t
ORIGIN      
        1 aacatctttt gcaagaagcc ttttttcgtt tccttcagca aatctaactt acgttgatga
       61 agagcgatcg tatcatctag ctgcttgaag aaggcaccaa ttttttgttg ttcatttata
      121 tccggtggaa actgaaatgt cctttgttct ataccacctt tagtcacatg taccattgtc
      181 gttccgttag ttgattcttt aattttctct ttatcagtca atagccaagt atatagatac
      241 tgtttgtcca attccttaga taaaaattta agtttccaaa tatgataatg gtagattaca
      301 cgttcttcat tccacatttc aggaccgaag cttgttgccc ataaatataa gagatctcct
      361 ttattagcat acttgtcttc ctctaattct aagtttgaat aataccatct atcattagta
      421 ttaaaattac ctactcttaa aaccctatat tttccagaat caagtaattc ttgttgttta
      481 aaagctctac cattcaaaaa aactaccact tcccccaact tccgctcttc ccaatctccc
      541 gtaaatcctg gaaaacgaac ttctggcact tttgctccgt ttttagggaa cattttttgt
      601 aagaatcctt ttttcgtttc cttcagcaaa tctaattcgt gctggtgaag catgatggtg
      661 tgatcaagtt gtttgaaaaa tgaaccgatc ttgatctgtt cgtcataagt tggaagtgag
      721 ataggtgtct ttttaccact atcaattgta taggttccga cggttccaat gcctgcaacg
      781 tcacggatat acttctgaat tttgttattt atgaacgagt agtaaaagaa gtttccgtca
      841 attttatgct cattcttgaa ccaaatgatg tttccatcct taaagtaaat tgggttatca
      901 tcagtgatta ataaggggac acctattgat ccgacaccag ttacaagcaa gtctccttga
      961 gaaactttac ctgaattttt cgaatattca ttatattttt cttctgaaat gtaaagataa
     1021 tcggatggtt cctcattttt tgcagctgag acaatatcac gagcacgtaa aaatctaata
     1081 cctgagttag tccaatctga ttgatgaatt cgcttcacag aagtaacatc catcatatct
     1141 cctaacttac gctcttccca atcttccgta aaaccaggaa accggatttc tggtcgtgta
     1201 tcattgctca tgtttgaaca cctccagcgc accttgtaac cacgcttcat tttcaggtgt
     1261 tgtttgcagg gaagagatag cttctaaaag ttctttttct agcacttgct gttcttgtcg
     1321 tatcttcttg atgtcatttc cgacatgaac catatcaatt ggttcttctt cttcgaaagt
     1381 atcgacatag cgtgggatat ttaagttata gtcattttct ttgatttcat caaaggttgc
     1441 caaatgagcg tatttctcga catcttttct ttcagcgtag gtttcgagga ttttttgaat
     1501 gttttcttcc gataatttat tctgattttt ccctttaaca aattcacgac tagcatcaat
     1561 gaataataca tctcgagttt gacgattctt cttcaataca atcaccgtcg ttggaatcga
     1621 tgtaccaaag aacaagttcg caggcatacc gatcacagca taaatactgc cgtcttctaa
     1681 taatttttga cgaatgaccc cttctgcagc accacggaat aaaaccccgt gtggcaagac
     1741 gatagccata gtccctgttt ccttcaaatg atagaaacca tgaagaagaa acgcaaaatc
     1801 tgctttggat tttggcgcta atttcccgta gcgattaaac cgagaatcat ctaagaaggt
     1861 cgtatctgct gaccaattag ccgaataagg tggattcatc actactgcat caaatgtgta
     1921 aggttcatct gttggccagt ccttattcaa cgtatcccca ttgcggagat tcatttcttc
     1981 ggcatctacc ccatgtaaaa tcaagttcat ttttgctaga ttgtatgtcg ttgtatttag
     2041 ttcttgccca tgatatttaa cgttatctgg atgagttaag tagttacgta catttaacat
     2101 caaggaacct gaacccatgg ttgggtcaaa gacactaaag aatcgtcgtt ctttttgatc
     2161 caacgtcacg atttgtgcca tcatatcaga aaccatatga ggcgtgtaaa attctccggc
     2221 tttcttccct gcttctgaag caaattgact gatcagaaat tcatacgcat ccccgatcac
     2281 atccccatcg tgacctaaaa cttccacgtc attcaatttc ttgataactt cggtaatcgt
     2341 cacatttcgt tgttgctcat ctgttcctag tttttttgat tggagatcca catcatcaaa
     2401 taacccatta aattgattgt aggtagatgc caattgcaca aaggctttgt ttagatcgtt
     2461 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaa
//
LOCUS       DAJSBN010000146         2454 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJSBN010000146 DAJSBN010000000
VERSION     DAJSBN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.151"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            132..344
                     /locus_tag="ONT86_002905"
     CDS             132..344
                     /locus_tag="ONT86_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353611.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="ONT86_002906"
     CDS             506..1024
                     /locus_tag="ONT86_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR2353612.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="ONT86_002907"
     CDS             1031..1543
                     /locus_tag="ONT86_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353613.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1814..2437
                     /locus_tag="ONT86_002908"
     CDS             1814..2437
                     /locus_tag="ONT86_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2353614.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          944 a          310 c          408 g          792 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat agattggata
     1801 ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc aaagtttagc
     1861 tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg aagacgattt
     1921 aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag agctgcaaga
     1981 gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg atcgattagc
     2041 aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag gtgcttctat
     2101 tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa gagacttgct
     2161 aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga aaaaaatcaa
     2221 agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata ctggtaaaaa
     2281 gattcaatac catgccaatg caacaggaaa aaacaaattg atttacgatg aaattgttga
     2341 tggtctgaag aaaaaagaaa ctatttctaa aatagcaaag aacgcaggag ttacaagaaa
     2401 aaccgtttat cgagtaaaaa gagatttagg aatgtaatat gttccagtta gaaa
//
LOCUS       DAJSBN010000147         2441 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJSBN010000147 DAJSBN010000000
VERSION     DAJSBN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.152"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            221..838
                     /locus_tag="ONT86_002909"
     CDS             221..838
                     /locus_tag="ONT86_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2353615.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            879..1178
                     /locus_tag="ONT86_002910"
     CDS             879..1178
                     /locus_tag="ONT86_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCR2353616.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1212..1379
                     /locus_tag="ONT86_002911"
     CDS             1212..1379
                     /locus_tag="ONT86_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2353617.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1424..2029)
                     /locus_tag="ONT86_002912"
     CDS             complement(1424..2029)
                     /locus_tag="ONT86_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2353618.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(2289..>2441)
                     /locus_tag="ONT86_002913"
     CDS             complement(2289..>2441)
                     /locus_tag="ONT86_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR2353619.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKV
                     LMGITA"
BASE COUNT          792 a          407 c          457 g          785 t
ORIGIN      
        1 atggcagacc gttaaggcct gccattttta tgtattttag cggaataaat ataatcatgc
       61 gggagtgtaa acttactgtt tcaaaaagta tactttttga catgccttga ttttctttgt
      121 ttatgtatgg cataataacc acataataca atttgaaacg tttctgattt aatctgattg
      181 ttttgaaaca attttagtga aaaaagggta gataaaaacg atgagaaaat ttttttatat
      241 ccgggtcagt agttatgatc aatcgatcga acgacaactg atttccgcga aggaattagg
      301 aatccctgaa gaatatatat ttgtagaaaa agccagcggc aaagatttta aacgccctga
      361 atatcaatta atgaaacgga tgtttcgaaa aggagattta ctctatctac aatcattaga
      421 tagactaggc agaaataaac aaatgatttt agaagaatgg cgagaattaa ctaaggaaaa
      481 gcagattgat atttctgtgt tagacatgcc tctgttagat actgctagtt acaagcaact
      541 agatgggtta gagacgctta tctctgactt aattctgcaa ttactcagct atatggccga
      601 agacgagcgg aaacgaatta atagccggca aagagaaggc gtagaagctg cgaaacaaaa
      661 gggtgtaaaa ttcggcagaa agaaaattgc tttacctcct gaatttcctg cgatctattc
      721 tgactggaaa gcaggaaaga taactgcagt ctatgctatg gatcaattgg gcatgaaacg
      781 aaataccttt tatcgacggg tgaaagaata tgaacatacc ttgcaccact tttcttgata
      841 gatataacag ctattagacg ggaagggaga gtttgaaaat ggacttaacg aatcaaaggg
      901 tcgtttttac tggaacgctg cagcaaatga cacgaaccca agccatcggt ttggttcact
      961 ctttaaacgg ccattgtcaa tcaagtgtta cttcaaaaac aaatttcctg gtttgtggtc
     1021 agtattctaa gtcgttgttt gatgattctc tctatacaaa aaaggaacaa accgcgcggg
     1081 acttgattgc gttaggtcat gagattacca ttttatcaga agtcgaattt tatgcgttaa
     1141 ttagtcaaca gttgaaagaa tggcaacggt atttataatc gttagtaaaa tttacaagga
     1201 ggaaaatttt tatgaagcga aaatcttatg atcgttgggg cattgaaata actagtttta
     1261 atcgaaatat tgcttttcac gtggagtgtg actgtgggga attagcaaaa agtgtttatc
     1321 aaaaaaatta cttttactgt cctaaatgcc atcgccgata cggggtacat ggaacataga
     1381 aacgaaatag tgcggtagaa aagcgaatga atgtagtata tttctacaaa tcattcgctt
     1441 tctttatttt ataaatagtt ggtcgagaga tcccgttttc ttttgcaatc tctgcaactg
     1501 gaatgccttg ctttaacatg tccaccacgg ttttaaaaac taagcgtttt tgcggatctg
     1561 ttgagttaat agaatactcg attggtcgtc ccttatatcg gccggctttt tgagccaata
     1621 atactccttg ccgctgacgt tcacgaattt tctcacgctc gttttgtgca atataactta
     1681 gcagtgataa aaacatatcg aacatagcgg tatccaggag ttcatttcca gtattaaaat
     1741 ctaaaaactg tgcatctaat atttttaacg ctactttttt ctgtttcata aaagcaactg
     1801 tattttttat atcttcataa tctcttccta aacgatctag tgatgtaacg atgacgcaat
     1861 caccttcacg gatataattt agtaattttt gaaactcagg acgctcagta tctttaccac
     1921 tggctttttc ttggaataat ttatcaatgt taagtccttc catcattgcc aattgtcgat
     1981 ccaaattttg atcttgtgaa gatactcgaa tatagccaat ttttcccata aaaaactcct
     2041 ttttaaaagt gatataattt acatgtaaaa tttacttgta gttttactgc tatcatagca
     2101 tttttaaaaa taaattttat gtagacttaa aatttacttc atggagggtt taaatggatg
     2161 aaatcaaaag taacaaaaaa gcatttggca tttatttagg agaacctagt acagaacaat
     2221 taaaccaatt ttttggttct gttgcgaagt ttgaaaatca aacgcgccct ctctgaactc
     2281 caaatatctt aggctgttat tcccattaat accttgattt cagtagacac cgaaaagccg
     2341 aagagcgttc catttcttcg gttcttttta tatattcctc gaatggtctc catgccctta
     2401 atcgtggaag aggctgtacg gagactttga taaaatttat t
//
LOCUS       DAJSBN010000148         2408 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJSBN010000148 DAJSBN010000000
VERSION     DAJSBN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.153"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            6..1151
                     /locus_tag="ONT86_002914"
     CDS             6..1151
                     /locus_tag="ONT86_002914"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR2353620.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1322..1780
                     /locus_tag="ONT86_002915"
     CDS             1322..1780
                     /locus_tag="ONT86_002915"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR2353621.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1939..>2408
                     /locus_tag="ONT86_002916"
     CDS             1939..>2408
                     /locus_tag="ONT86_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCR2353622.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          456 g          698 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgttag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca taaagatcta gccgatcaga
      661 tcggatttta ccaaaatgct atcggagccg tacttggtcc ccaagatagc tggttacttc
      721 agcgcggaat caagacacta agtgttcgta tggaggaaca tcaaaaaaat gcttttgtgg
      781 tagctgattt tctattttct catccagcag tagaaaaggt ctactatcca ggattacccg
      841 atcatgaact ccatggagtt gctaagcagc agatgagcgg ctttagcgga atgatttctt
      901 ttactttaaa aaatgaagaa tctgctattc catttgttga atcactccaa ctatttactt
      961 tagcagaaag tttaggtggc gtagaaagtt tagtggaaat tccttctgtc atgactcatg
     1021 cgtctattcc aaaagaaaaa cgagaagagg ctggcataaa ggacggcttg atccgtcttt
     1081 ctgtaggaat tgagtatggt caggatctca taaacgattt agctcaggca tttgacagaa
     1141 tcaagaacta gaagtcccaa taaacgactt agcagaaaaa tctaaaaaat atttcagatt
     1201 gctacacctt ggaaacgata agtgttagaa tcaagtagaa agacgttttt gtaaatttca
     1261 ctaaataatt ctttagtgaa aagcatcaaa acgaatgaac aaacagatag gagtcttaca
     1321 aatgagaatg aatgtcgaaa gttttaatct tgaccataca aaagtcaaag caccttatgt
     1381 acgtgtagct gatcgaaaaa cgggtgcgca tggcgatgtc atcattaaat acgacgtccg
     1441 ctttaaacaa ccaaataaag aacacatgga catgccaagt ctacattcac ttgagcactt
     1501 gactgccgaa ctaatccgca atcacgcaga ttacatcgta gactggtctc caatgggatg
     1561 ccaaacaggc ttttacttga ctgtattgaa tcatgaaagc tacgaagaca ttttagatgt
     1621 tcttgaagca acaatgaaag atgttcttga agctactgaa gtacctgcaa gcaatgaaac
     1681 acaatgtggc tgggctgcca gccatacact agaaggtgca caagctttgg ctcgtgaatt
     1741 tttagcaaaa cgcgatgaat ggtctgaagt agaagcatag tttctaaaag aacgtaaaga
     1801 tgagaagcaa tatagctatc ttatcttcgt attttattaa atatttattg tctgaaaaga
     1861 gttctaaacg ggaaaacaaa tagctagttt tcagtttagc gctcttttta ttatataaaa
     1921 tagaaaggag aaaactttat gtctatttat aattttgaaa ctactttaga agacggcact
     1981 gtctattcat tgaaaaaata tgcaggcaaa ccgataatca ttgttaacac tgcaacaaaa
     2041 tgcggtctag cacctcaatt taaacaatta gaagaacttt accaagctta tcagacacaa
     2101 ggtctggtta tcttaggctt tccttccaat caatttaaac aagagctaga ttcagcgact
     2161 gaagcagcag aagcttgtaa aaccacttat ggcgttacct tccctatgca tcaattgatt
     2221 gacgtaaatg gagaaaatac agatcctctt tttacttatc ttaaaaatga agctcctggt
     2281 actgtcacta aagcaataaa atggaatttt actaaatttt tgattgatag aaatggcaaa
     2341 gtaatcgaac gatttgcacc acaaacttct ccattgaaaa tgaccgctgc aattgaatcc
     2401 gttcttta
//
LOCUS       DAJSBN010000149         2398 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJSBN010000149 DAJSBN010000000
VERSION     DAJSBN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.154"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            207..2069
                     /locus_tag="ONT86_002917"
     CDS             207..2069
                     /locus_tag="ONT86_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2353623.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ONT86_002918"
     CDS             2069..>2398
                     /locus_tag="ONT86_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2353624.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAJSBN010000150         2240 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJSBN010000150 DAJSBN010000000
VERSION     DAJSBN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.157"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(36..2120)
                     /gene="cadA"
                     /locus_tag="ONT86_002919"
     CDS             complement(36..2120)
                     /gene="cadA"
                     /locus_tag="ONT86_002919"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR2353625.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT          646 a          509 c          355 g          730 t
ORIGIN      
        1 ttcattaaga aaaggatata aaaaaaagag gacgttcatt cgtcctcttt ttttacatgc
       61 aagcttcgca tggcattcaa aacagctaat actgtcaccc caacatctgc aaatactgca
      121 tctcccattg atgcaaaacc aaaagcacct aaggcaagta ccaaaatttt tatcccaata
      181 gcaaagacaa tgttttgttt gacgattttc aatgttttct ttgctaaatg gattgcttct
      241 gcaatcttcg atggttgatc attcatgatg actacatctg ctgcttcaat tgcagcatcg
      301 cttcccaatc cacccattgc gattcctaca tctgctcttg ctagcaccgg tgcatcgttg
      361 attccatctc cgacaaatgc ggtcttttta ttgtttcttt gtaagatttc ctctaaccgc
      421 tgcactttat cttcaggaag caattcgcta tatatcttgt ccacgcctac ttgcttcccg
      481 atatgatcag caattttttt cgagtcgccc gtcaacataa ctgtgttctt cacaccagcc
      541 tgttttaatt gaaccaatgc gtctacggca tcttctttca aggtatctgc aataacaaga
      601 taaccactgt aactttgatc gatcgctaga taaagcaatg taccgatttc ttgacttaaa
      661 ggaaaagaga tttcaaatct ttccatcaat ttgtgattac ctgctaatac ttgtttgcca
      721 tcaacttcag ctaagatccc ttcacctgca aattcctgga tattagttgt tggtaaaagc
      781 ggacctgcgt agccttctaa aacagattga gcaatcggat gtgtagaaaa ctgttctgca
      841 ctcgcaatat attgcatgaa tttctttgga tcgatttttg tatcgatcgt ctggatcgaa
      901 aagtcacctt tcgttaatgt gccggtttta tcgaatacca ctgtctcggt ttgcgcaagc
      961 aactccaagt aatttgaacc ttttactaat acaccgattt tacttgcgcc tccgataccg
     1021 ccaaagaaac taagtggcac ggaaatcact aatgcacatg gacatgagat cactaaaaat
     1081 gtcaatgctc gatagatcca ttcagaaaac gccgctccag aaacaacaag tggaggtaaa
     1141 acagctaata aaacggccaa tcctacaacg actggtgtgt aatagcgcgc gaatttagta
     1201 atgaaatttt cagctggtgc ttttttactg ctggcatttt ctactaattc taataatttg
     1261 ctgatagtag aattttcata agatttttct acttgaacta aaaatggttt atttttattg
     1321 ataaatccgc ttaatactgt ttcaccaaca tatacagatt tagggactga ttctcctgta
     1381 agtgctgacg tatcgactaa tgattctcct tcgatgatcg ttccatctaa cggtacacgt
     1441 tcaccaggct tcacaagtac aaactgaccg atctgcacgt cttcagggtt cacggtcatc
     1501 aatccttccg tcgtctgcac gtgagctgtt tctggacgga tcgccatcaa ttcgcgaatc
     1561 gattttcttg aatgactgac tgccaatcct tggaaatact cgccgatttg ataaaatagc
     1621 atcactgcta cggcttctgg atattcaccg atcaacatag cgccaatcgt agcaacagac
     1681 ataagaaaat tctcatcaaa gatttttcct ttgacgatat tcatcactgc cttcttcact
     1741 acatcatatc ctatcagcag ataaatcaat aaatacccta tcaagcgaat tggttcatta
     1801 aaatcaaata atagcaataa agcgatacct gcaagggaaa ataaaatccg gaacagcgta
     1861 tttctctctt tttcatttcc atgttcagat actttttcct tatctatttc tgttactttg
     1921 acatctggtt ctaattcctt cactactttt tttgtttctt gttcaatcgt agccaaatga
     1981 tcttcctctg cgtcaatcgt tagttttcca gaagtgaagt ttaccgttgc ttccttgact
     2041 ccattgatct tctgaactcc tttttcgatt ttcattgcac agttcgcaca atctaaacct
     2101 tctaatctgt aacttttcat tttaatgacc tcctcgcctc aacatatgaa catctcttca
     2161 taactaatta taaatgaatg gttattcata tgtcaaggta tccgaaagaa aaaatgaaaa
     2221 acatctgcat cggaaaattt
//
LOCUS       DAJSBN010000151         2232 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJSBN010000151 DAJSBN010000000
VERSION     DAJSBN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.158"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..544
                     /locus_tag="ONT86_002920"
     CDS             <1..544
                     /locus_tag="ONT86_002920"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2353626.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="ONT86_002921"
     CDS             complement(900..1472)
                     /locus_tag="ONT86_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2353627.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="ONT86_002922"
     CDS             complement(1488..2093)
                     /locus_tag="ONT86_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HCR2353628.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          700 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt ct
//
LOCUS       DAJSBN010000152         2206 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJSBN010000152 DAJSBN010000000
VERSION     DAJSBN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.159"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..414
                     /locus_tag="ONT86_002923"
     CDS             <1..414
                     /locus_tag="ONT86_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR2353629.1"
                     /translation="GVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYI
                     LRYDDKIIEIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDM
                     YEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            415..1167
                     /locus_tag="ONT86_002924"
     CDS             415..1167
                     /locus_tag="ONT86_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR2353630.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1172..2119
                     /locus_tag="ONT86_002925"
     CDS             1172..2119
                     /locus_tag="ONT86_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR2353631.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          783 a          286 c          427 g          710 t
ORIGIN      
        1 ggagtagaat tagactgtat acatgaattg gattatatta taaacctatt tggtttccct
       61 aaatcagtaa aaaaacagtt tggtaaatat tcgaatcttg aaattaacag taatgattta
      121 gcaacatata tattgagata cgatgataaa attatagaaa ttcatttaga ttattttgga
      181 agagttccta caagaaaatt tcaattgttt actttagatg aatgtatcaa tgttgactta
      241 ttaaataata aaataagttt tgaagaaaac attgttaaag aaatgcctga atctgctaat
      301 gatatgtatg agaaggagtt gaaatatttc tttgaaaacg tagtaacagg caaagaaaac
      361 tggaatgatt tatatcatgc aaacgaagta ttgaaaatag ctgaggagaa ataaatggaa
      421 attttattta ctatttgtgg tagagctggg tctaaggggt ttaaaaataa aaatttgaaa
      481 tatttggcag gaaagcccct agtccatttc gctttagcaa tgatagatat ctatcaacaa
      541 aaacatcctg aacataattg cacgacttgt ttgaatacgg acagtaatga gttagtagaa
      601 ttgacgacta agttaaatcc gtctgtgaaa attgtagaac gtaaacctga attagctaca
      661 gatactgtag ggaaaattga agtgatacgt gattcattct tacaactaga aagagaagat
      721 aatatatttg atttaattat agatttagat ttgacttcac ctttacgtga agtttctaat
      781 cttgaagaat taatagatga acatctaaaa aatttagata aagacgtcta cttttcagtt
      841 gtttctgcaa gacgtaatcc ctattttaat atggtaaaaa gaattgaaaa tgaagttact
      901 atagttaata aatcaagata cacatctagg caacagacac ctgaagtttt tgaaatgaat
      961 gcctcaatgt attcttttaa acctgaattt ctaaaaaaaa gtaattatat ttttgatggg
     1021 caatgtggga taataaaaat gagagattat ctaattttag atattgatag tgaagaagat
     1081 tttaaatggt tagaattttt gtttcctaaa tttttagaag attcttcgga gcttcaagat
     1141 atctataatt atttatatcg gaggtaaaag gatgagctgt tatcttatca ccggtggggc
     1201 tggcttcatt ggttctacct tagccaatca tttaggagaa gaacatgtgg taatcgtcgt
     1261 cgatgactta tcaatgggaa aagtagaaaa tcttgatatg acgaaaaata tcacttttat
     1321 tgagggagat gtcgctgaga aatccttgat ggaagagatc atgaaagcta ataaatttga
     1381 ttatatcttt catttagcag cagttgctag tgtggcagat tcagttgccc gaccagtaga
     1441 aacacatcgt gtgaattttg aaagtgtatt aatgatttta gaattgatcc gcaagtatca
     1501 acctgattta aaacggatcg tgtttagttc ttcagcggct gtttatgggg atgaaccgac
     1561 tttaccaaaa aaagaagagt ccgtgattcg cccattgacg ccttatgcgg ttgataaatt
     1621 cgctgcagaa caatatgtgc tagattattg tcatttatat gatgtacccg gtagcgcagt
     1681 tcgttttttc aatgtctatg gtccaaatca aaatccgaat tctccataca gtggtgtgat
     1741 ttctattctc gtagatcgct ataagaaaca aatcgtagga gaagcaactt catttacatt
     1801 atttggtgat ggaagccagt ctagagactt tgtttatata gatgatgtaa tccaagcatt
     1861 acttttagtt gcgaatgagg aagctgcact ggggaaacaa ttcaatgttg gaacgggtaa
     1921 agcaaccaca cttttggaat tgattcatgc aatcaatgaa attctcggtg tagaattgac
     1981 acttgaatac aaagaagagc gttcgggtga tatccatgac tctctagcag atattactaa
     2041 gatccagtca attggctatc aaccgaaata tggtatttta agtggaatga ctaattattt
     2101 gaaaactgaa atcaattaaa acatactaag ataagattag agggtaaatt caggctcttt
     2161 gtcaataagg actgatgagt tttgtaaaat caaatctggg atagaa
//
LOCUS       DAJSBN010000153         2173 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJSBN010000153 DAJSBN010000000
VERSION     DAJSBN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.160"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..378
                     /locus_tag="ONT86_002926"
     CDS             <1..378
                     /locus_tag="ONT86_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR2353632.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="ONT86_002927"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="ONT86_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR2353633.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..2026
                     /locus_tag="ONT86_002928"
     CDS             1859..2026
                     /locus_tag="ONT86_002928"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353634.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            2080..>2173
                     /locus_tag="ONT86_002929"
     CDS             2080..>2173
                     /locus_tag="ONT86_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR2353635.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREV"
BASE COUNT          613 a          362 c          469 g          729 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttactggttc tgttgcaaag ttttaaatct actatcaaat
     2041 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt taaaggaaag
     2101 caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta taaccttagc
     2161 tatcgtgaag ttc
//
LOCUS       DAJSBN010000154         2154 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJSBN010000154 DAJSBN010000000
VERSION     DAJSBN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.161"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..914)
                     /locus_tag="ONT86_002930"
     CDS             complement(<1..914)
                     /locus_tag="ONT86_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCR2353636.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ONT86_002931"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ONT86_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR2353637.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          729 a          381 c          416 g          628 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagc
//
LOCUS       DAJSBN010000155         2057 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJSBN010000155 DAJSBN010000000
VERSION     DAJSBN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.162"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(387..1853)
                     /locus_tag="ONT86_002932"
     CDS             complement(387..1853)
                     /locus_tag="ONT86_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2353638.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          644 a          412 c          341 g          660 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgc
//
LOCUS       DAJSBN010000156         2005 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJSBN010000156 DAJSBN010000000
VERSION     DAJSBN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.163"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..1023
                     /locus_tag="ONT86_002933"
     CDS             1..1023
                     /locus_tag="ONT86_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HCR2353639.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            1045..>2005
                     /locus_tag="ONT86_002934"
     CDS             1045..>2005
                     /locus_tag="ONT86_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2353640.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKM"
BASE COUNT          806 a          214 c          381 g          604 t
ORIGIN      
        1 atgacacaga aacattcaag tataaaaaga attatacatc gcatatccga agaattgaat
       61 gcaacaagac gaattgcaaa gataacatct tggaaggaag catttgccac gttacaagct
      121 aaaattgata ttcaagtcat gaatcgtaat ggatataatg aaagtaaaaa acagaaggaa
      181 catttattaa aaaaacatga ggtaatgctg aagtattatg aaaagacttt ttcagatttt
      241 ttgaaaacat atgattatag tcaaaatcaa ataattaatc aatcaaaatc accatattct
      301 aactgcattt gggtttgttg gtggcaggga ttggatcaat cgccggaaat tgtaaaagct
      361 tgtgtaaaat caataaaacg aaatgcaggg aaacatactg ttataatttt aacagaagat
      421 aattatagac aatatgttga tataccaaaa tgggtggaag aaaagaaaaa caaaggaatc
      481 attacgagaa caaattattc tgatttatta agattatctt tgctagcaaa acatggaggt
      541 ttgtggttag acgccacatt ttattgtaca aaaccaatac cagatgagta ttttgatatc
      601 gatatttggt ctattaggag gcctgattat gctcacgcat ccgtagcatc aggatatttt
      661 gcgggttatt cattagcatg taatcaagag tctagatgga tgtttgctat aattagagac
      721 ttttttttaa attattggaa aaataatgat atgatggtgg attatttgat gatagattat
      781 atgattgttt tagcacagag aataaatcat gaaatagcac agacatttga taaaatacct
      841 agtaacaatc cattatgtga cgaattgatt aaaaatttaa ataatatata tgatgaaaaa
      901 gtatggaact tattaaagaa agatacttta ctgtttaagt tgacatggaa acaatctttt
      961 aaaaagcaaa aagatgataa agttactttt tatgggatgt tggtagaaaa caaattatta
     1021 taaatattag aggtataaaa agttatgaaa cacaaggtaa gtgtgatagt accaatttac
     1081 aatgttgaaa aatatttaag aaaatgtgtg gatagtttat tgagtcagac attaaaagag
     1141 atagaaatcg tattagttga tgatggttcg ccagatgctt cgggggaaat agcagatgag
     1201 tatcaaaaaa aatattctaa tgtaaaaaca atacatagag aaaatggggg gttaggaccg
     1261 gcaagaaata caggaattga gaatgcgaca ggagaatatg tggcttttct tgattcagat
     1321 gattgggtag aaagtgatat gtatgaaaaa ttatacttag ttgcgtccaa agaaaatgct
     1381 gatatagtag ttagtggaca ttgtgatttt gcgaatgaca aagctatggt gaaaaagcaa
     1441 catcctttga gtgggaatgt atatgatacg cctaatagta ttaaaaatgt acgaaaaaat
     1501 ttatttggac atagtccaaa agatcaagaa gtagaggcct tcccgatgtc agtatgtatg
     1561 tctttgtata ataaaaatct gctagataaa tatagactaa gatttgaaaa aattttatct
     1621 gaagatacaa tttttaactt gaatgcatat gatgtcgcaa ataaaattgc ttttacagaa
     1681 tatacagatt attgttatag aaaagaagaa caagagtcta ttacaaaaag tttatctcca
     1741 aataagcaga cggaatttaa agaattttta ataactctaa aagctatagc atcgaaagaa
     1801 gatttagaat gtcaaatcag agcaaaacgt atggcaattg attattgtag gctttatgtg
     1861 ggcttagttg atgatgcaaa tttgggaatt aaagagaaaa gaaagtatat aaaaaaattt
     1921 attgaagatg agggattaat tcaatgttgg aagggatatc ctacaaatac gctaccattt
     1981 caacaaaaat tttttcataa aatgc
//
LOCUS       DAJSBN010000157         1952 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJSBN010000157 DAJSBN010000000
VERSION     DAJSBN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.164"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(7..1434)
                     /locus_tag="ONT86_002935"
     CDS             complement(7..1434)
                     /locus_tag="ONT86_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR2353641.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            complement(1427..>1952)
                     /locus_tag="ONT86_002936"
     CDS             complement(1427..>1952)
                     /locus_tag="ONT86_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR2353642.1"
                     /translation="RINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWW
                     ALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPL
                     ELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLN
                     RIDKEILKVGNIDE"
BASE COUNT          735 a          318 c          229 g          670 t
ORIGIN      
        1 ttcatattaa ttctcctttt ttaaatacaa gtaagaaatg cttcccaaat aataccagaa
       61 aacaattgaa atgacatcgc gcttattgaa aagtacatga gcctccacca tttcatctat
      121 aaaaatagat agtataaaaa ttaaaacaat accaagaaac gcataattca ctttacttct
      181 aaaaaatgtt ttgagtaaac taaagatatt taacacatag aaagcataga tacatataaa
      241 agcaattata ccacttgata caagtacttg aagaaatcca ttatgcatgt ttccatgtgc
      301 tctttttagt tcattcacat ctgtagtatc aagatctact tttttcaaaa attttgcatc
      361 gctattccca tagaaatcgg tctctccata accaaataac aaagttggtc ttataacttt
      421 aagcgcagct ttccaaatcg taaaacgacc agctgaaatt tcggagttat cactagaatg
      481 ctgtaatatt acttcttttt ctggaagtgg agttttatct gattcatttg actctataac
      541 ttctactttt ctatcaatcg atacaggaat tgttgctaaa aaatccttag cataattagt
      601 accaaaagaa aaatatacca atagaactaa taatatgctt accatccatt tgatttttac
      661 aggtagtact tctctaatgc tctttctaaa ttttgaattg gcaaacaaca aaagaaataa
      721 tactaaaaaa gaacaaatta ctacctgggc acctctagac gaagaaagtg taatataaaa
      781 tatttgtacg ataaaattca tcatataaaa aacacgaatc gtttttgtta tttttgaaat
      841 taatacacta attaaactta aaataataga agtatatcca aacatagctc caacatttgg
      901 acttgtatag acaccaaata accgatgttc aataaaacct tgtcttgcaa gtaatcctgg
      961 cgttcctgtt gggacatgat aagaaatcaa aaaaataaat tgatagatag atattagcgc
     1021 agctaaaaaa attatgataa tgaaaacatt attaaaatac accatttttt tctttttttc
     1081 ctcttcactt tgtgaaaagt ctgcaggaaa aattactatc aaagtaatca gcaaatatcc
     1141 aaaattataa atagatggaa ttaaaaaatt ttctctgtta atgattatag ttaagataaa
     1201 actaatcatt gctccaatca gtagccaaga aaaacgcatt ttaaaaaaat ttttctttat
     1261 aaaaacctca tacaaaacta taattccacc ccaagcaaaa gctattttca tcgggatatt
     1321 agttacatta aaagtactag gcataaaata aagcagacaa atgattaaat ttattactcg
     1381 aaacgcatct cgacttaata aactcttctc taaaacaggt actattctat tcatctatat
     1441 ttccaacttt caaaatttct ttatcaattc tatttaatag tttatcagag taatccgatt
     1501 taacttttct tacaaataat ttttcacttt cttttatttg atctatatcc ctttcatcaa
     1561 accatttgtt catggaagga tcaataatat gaatatttgc taatttcgaa gtttgtaaag
     1621 tagtttcttc aatatttttc aattcaagtg gtaaatctgg tcctccaata acatttttat
     1681 ttttgaacga ggaattaaaa aaaagtgtat gaaaaaattt ttcatctggg gcaaacatac
     1741 ctattttaaa attcaggtag tcttgttgat tttgttcata catttgtatt aattctgtta
     1801 ggcattcttt cgacaaagcc caccattgag aaccatgata caaatcaaat ttatggtgtc
     1861 taatttttgg gggaagttta atcatcctca gacaacgatt aattatagct ctaaaaattt
     1921 taaatgcagt tgtgttattc ctattaattc ta
//
LOCUS       DAJSBN010000158         1847 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJSBN010000158 DAJSBN010000000
VERSION     DAJSBN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.165"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(1..634)
                     /locus_tag="ONT86_002937"
                     /pseudo
     CDS             complement(1..634)
                     /locus_tag="ONT86_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1119..>1847
                     /locus_tag="ONT86_002938"
     CDS             1119..>1847
                     /locus_tag="ONT86_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353643.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIE"
BASE COUNT          577 a          290 c          293 g          687 t
ORIGIN      
        1 aggtaaaaaa taagtctgat tccttgtctc taaaggcttc ggtcagctca tggaaaaaag
       61 tataggcgtc ctttaaaggc gttgaaaatg ccagcaatcg atcaatcatc attgcttcag
      121 tgagtagcgg gtatttaggt gcacgaaaac tgcgccatgt tttgtaatca tagtgattga
      181 ttttttgacg gttttttaga agaaagcgcc agttcttttt cactttctcg gcttcccgtc
      241 gttgccctaa ctggtgtaat cttttcattt cttgtacacg aaaatcattg aacgcctgat
      301 tcaaatgttt gatgatatga aaacaatcaa tgacgacttt cgcatttggt aagacacgtt
      361 ttgtcagttg gaagtaggcg gcgttcatat ctgtcactaa atattccacc gcttcaggat
      421 ttgcacagcg actaaaatag tgtgttaatc gagacaattt tcgtgtaggc aaaatatcaa
      481 tgagttttcc agtctcgcca tccgcacaaa taaagctcat tttatccaca attgaagcgt
      541 gcgaacgaaa ttcatccacc atcaatactt ttggcagggt cctttttgac ggcttcggaa
      601 gatagttttt caactctttc aatgtacgaa tcatatacct aaaataacaa tgggactgaa
      661 gaatcaattt tgattcatca gtcccataaa ttatagagcc tatattttgc gaattgcgct
      721 acggtttctt cttttctttt gtctgtaata tgaatatata gatccataat gatttgaata
      781 gaagagtggc ctaaacgctc ttgcacatct ttaatatttg cactagcttg ctaaaagcaa
      841 gctagcatgt gtgtgtctaa gaccatgaat agtaattcgt ttaagattgt ttttcctgat
      901 gattacatct aaccattttc gagatttaga taattgaagg tgttcatttc tttctggtaa
      961 aaatttaaat atataactac attttctcta ctaactttga aacactactt ttgtaatata
     1021 aaaaatattt tattaataat aaaattaatc acattttata tagtgagtaa tctagtgttt
     1081 taaaaaaata aatgatacta tatgtaatat aaaaaaacat gaggtggtat atgaatttga
     1141 aaaaaatttt ttctgtaaaa atatttatag tttatgcatt tgttagttta ttatatttat
     1201 ttccagcatt taaaaacatg gaaatatttg gcttttatga tacggcattt cacattaatc
     1261 gtgcgttaag tttagaatct atttttagct cccctattaa ttttgaaacg tttagatcat
     1321 atggcatgca agtaaataat ttttacccat ggttaactct atatccttta tttttattga
     1381 tcaagtttac taatttagca ataggctata acctttttct ttatattgtt acgctcatta
     1441 ctctatttat atgtcattat gtaatgtatg aaattaccaa aaaacatgtt actagctcct
     1501 ttttcgctat tatatataca acatcttctt ttaggagtgt agaaatattt cttcgagggg
     1561 caatggggga attactcgca atgtcaatac ttcctcttat tttactcggg tttataaagc
     1621 tttacgactc aaaaaaagaa tcttgggtta tgttggcgat tagcatgact ttattaattt
     1681 acactcatgt tctttctgta gctatgacta tggtaatgat tataattgct ttaattattc
     1741 aattttatcg gaaaaagaaa atagaaatct tcttaattat taaattaata aaagctgcta
     1801 tagttacttt acttctttca ctagcgttta taggtcctat gattgag
//
LOCUS       DAJSBN010000159         1796 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJSBN010000159 DAJSBN010000000
VERSION     DAJSBN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.166"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="ONT86_002939"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="ONT86_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR2353644.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAJSBN010000160         1771 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJSBN010000160 DAJSBN010000000
VERSION     DAJSBN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.167"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..500
                     /locus_tag="ONT86_002940"
     CDS             <1..500
                     /locus_tag="ONT86_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353645.1"
                     /translation="SIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGK
                     KETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFE
                     KTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLV
                     EYGII"
     gene            487..>1771
                     /locus_tag="ONT86_002941"
     CDS             487..>1771
                     /locus_tag="ONT86_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR2353646.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFV"
BASE COUNT          580 a          228 c          277 g          686 t
ORIGIN      
        1 aatcaatctt tgggatagaa gaagtttttg tcggagataa atgtatcttt tttgatcaac
       61 ctttattatc tgatggttat ggcatagatg aaacagatat tttcgctaaa gtaaatagta
      121 tattttctgg aaaaaaagaa actttaatta agcttcatcc tagagcaagg acaaatcgtt
      181 atggtgaaaa aaacatattg aattccagtt taccgtttga agtcttgcta cttgaaaatg
      241 acttctcttc aacacttttg attagtccta tatctacaat ttctttttca ccatctatga
      301 tgtttgaaaa gacgatcaac tctattatgt tggctaaatt aattttaaat gaatatacag
      361 cattatctga agataaaaaa gaaacattga caaatattgt tcttttctgt gaacattata
      421 accgtctatc agaagaaaaa attttactcc cagaaaactg ggcagaatta agaaagttgg
      481 tagagtatgg gattatttaa cacactttcc aagtcaaaat tagttaagtc cagtttctgg
      541 tatactatag ggaatttttt tattaaggga atttcattca taactattcc tatttttgta
      601 aatattacta ctcaagcaga atatggtttg attaataatt tcacttcatt tgtatcaatt
      661 ttttcgattc ttgttgggct aagcctaaat tcggcaatta ataatgcaag ttttgactat
      721 aaagatgaca taaatgaatt tatgtcatcg gtactttctc tagctacact ttcgttaatt
      781 gtgttcttat taatagggaa catatatttt ttgttttcaa atgctttttt tgatatgaac
      841 caacttgtat ttaatttgat gttattacaa agttttgcta gttttgtaat tagttatatc
      901 cagtcttatt ttacgattaa ctttaaccat tttaaatatt tgattttatc ttttatatct
      961 acttttttga atattgcaat ctctgtagct ttgatgcaga cattatattt tgatgataaa
     1021 tatgttggca gagtagtcgg cggaacactg tcatttgtgt tgataggttt agtaatttat
     1081 ttaagaataa tgataaaggg tgctcatttt tttgataaaa aacagtggag atacgcttta
     1141 aaaatctctt tgccgctaat cccacattcc ttagcaaata tagttttagc acaattcgat
     1201 agaattatga ttaattctta tttaggtcct gctgcggggg gaatatattc ctacattagt
     1261 aacataggta ttattctaag cgtaatatgg atgtctacta ataacgcctg ggttccatgg
     1321 ttctatggtg agatgagtaa aaataatgaa aaaaaagtga agaaagttag taactattat
     1381 ttagtagtct ttactttgat tacgatgatt gtaatggtag tttcgattga tttagcaaaa
     1441 tttatggctc cgaaagacta ttattctggg ttatctttag tggttccgat aaccttaggc
     1501 tatttctttc agtttttata tagtttacca gttaatgctg aattttatga gaaaaaaaca
     1561 tcgtttattg cgattggtac agtagcaagt gctatagtta atatactatt aaacatgatt
     1621 ttcataccta ggttaggaat aattgctgct ggatacacaa ccgtatttac gtatttttta
     1681 ctttttattt ttcattataa tatcgctaaa aaaattacga ataaacaatt atttgacaca
     1741 aaaaaaatat gtatcataat aatttttgta t
//
LOCUS       DAJSBN010000161         1648 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJSBN010000161 DAJSBN010000000
VERSION     DAJSBN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.169"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            138..1604
                     /locus_tag="ONT86_002942"
     CDS             138..1604
                     /locus_tag="ONT86_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCR2353647.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          296 c          337 g          553 t
ORIGIN      
        1 tttttatgat attgccatta aaaattactt gcttttatag aaaattagtt tatatttctt
       61 aaataagttt tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa
      121 gaattggggt atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg
      181 gattgtcaac tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg
      241 cattacttat ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag
      301 caacagcatt agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg
      361 gcggatgggt atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt
      421 tcattatgct tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt
      481 cgatggcatt gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg
      541 gacatcttta ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg
      601 gtgtcaactt aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata
      661 actatcattt agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca
      721 tcatccaagg acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa
      781 ctgaagaacg taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag
      841 tattcggcgg ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca
      901 gtgtattggg gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg
      961 taactgctga agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca
     1021 ttttctggtc attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta
     1081 cacaatctac gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa
     1141 ttttcatctt tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc
     1201 aaccatctac agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc
     1261 tattgatgtt gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt
     1321 tgggaagttt cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg
     1381 tgacaacaaa attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag
     1441 ctgatgcttc ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag
     1501 aatctgctta cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta
     1561 ctgtgaaaga accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa
     1621 caggaattgc ttatttgcag cagttcct
//
LOCUS       DAJSBN010000162         1647 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJSBN010000162 DAJSBN010000000
VERSION     DAJSBN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.170"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..626)
                     /locus_tag="ONT86_002943"
     CDS             complement(<1..626)
                     /locus_tag="ONT86_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2353648.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
     gene            914..1567
                     /locus_tag="ONT86_002944"
     CDS             914..1567
                     /locus_tag="ONT86_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2353649.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          590 a          229 c          303 g          525 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt
      841 caccaacact agatattata gagccataat atgctgtata atatctagtg tataggctta
      901 agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat
      961 aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc
     1021 cttgtcaaaa tttaatagcg atataaattt ggttatctta gacattcaga taccttttat
     1081 aaatggttta gaagtgttga aagaaattag aaagaaaagc aatttaccaa ttctaatctt
     1141 gactgcattt agtgatgaag aatataaaat tgatgcattt actaatttag tagacggata
     1201 tgtagaaaag ccattttcat tgccggtctt aaaagctaga atagattctt taattaagaa
     1261 gaattatgga catttagaga agtttgaata taaggatcta tcggttaatt ttaatagcta
     1321 tacagctaaa ataaatggtg aagaaataga tgtaaatgca aaagaactag aagtattaaa
     1381 atgtttattg gataataatg gacaagtgtt aacaagaatg caaattattg attatgtatg
     1441 gaaagatagc gaagaaactc cttatgatcg agtaatagat gtatatataa aagaacttag
     1501 aaaaaaatta caattagact gtataactac tataagaaat gtaggatata aattggagag
     1561 aaaatgaaaa aattaaagat atttccaaaa atgttcatac aaatattttc tgttcttgga
     1621 ataataatta tattagttca ttcatta
//
LOCUS       DAJSBN010000163         1609 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJSBN010000163 DAJSBN010000000
VERSION     DAJSBN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.171"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..302
                     /locus_tag="ONT86_002945"
     CDS             <1..302
                     /locus_tag="ONT86_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2353650.1"
                     /translation="FMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            602..1294
                     /locus_tag="ONT86_002946"
     CDS             602..1294
                     /locus_tag="ONT86_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR2353651.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          683 a          196 c          231 g          499 t
ORIGIN      
        1 ggtttatgat agataaatgg aaaaagcagt atccacgagt ggttaaattg ctcatgaatc
       61 ctgctatatt aaccttttat aatttccctc cgtcaatcag aagaactatt tattcaacta
      121 atttgattga aggttttaac aaacaattaa agaaatatac caaaagaaaa gagcaatttc
      181 ctaatgaaga atctctggag agatttctag tatctcaatt caacaactat aatcaaaagt
      241 ttctatgccg agttcataaa ggttttaaag aaatacatga tacattagaa tcaatgtttt
      301 aacttgatca acagaatggg cttttccatt tacacataat tattgacaca acctattctg
      361 gaaacttaca aataaaagct tattcttcag ccaccttcgg gtggttattt tttataaaaa
      421 aatgtaaaaa aatattgata cataaataaa ttagttgtag taaatagttt tagaattgaa
      481 tttctctatc ctttttaaat aattttttag acaaaaaaca gaaagtgtct aaagaaagct
      541 tgacgaagaa aataattttt aataacttat ttaaggaaat tttttgaagg agaagataaa
      601 agtgagaaaa aagattatta ttaatgctgg agtaattata ggattgatgt tagtaatttt
      661 ctccggaaag caatatgctg ataatatttt attatgggga ggggaagata atattgaaaa
      721 gatcaacaac aatttaacta tattagacac agctctagaa aataaagagc aaaaaattag
      781 tattttaaat tcacaattgt cttcaaacaa ccaaactctt gaacaactaa gaactaatat
      841 tgaagtttcc aaacaaaaag tggcatcttt agaaaacgac aagaatcaat tagtatcaga
      901 gaaaactaat ttagaaaatc aattgtcagc aaaagactct gaattgcaag gaaaacaaaa
      961 tgaaattaat gcgaaaatag acgaaattaa ccaaaaagta gctgaaataa atcgattaaa
     1021 tagcgattgg aataatcgtt tacaaaatga aaagaacaaa tttaatgaag aacaatctac
     1081 tattaatagt ttaaaccagc aaattaatca aatgttgagt gaaaaaaata acattcaaaa
     1141 tcaactgcaa gctactcagc aaaagctaga agaagaaaag tcaaaaaata gtagtttgaa
     1201 taattacatt gaaaagcttc aaaaagctaa aaatgatgta gaggataccg caaataaagc
     1261 acaacaaatt gtaaatgaac atacaatcca atagatatag ttttaattta acaaatggta
     1321 tgtttggaga taatgagata gaatataaat gtcataatat tatacaatct aaacaccgtt
     1381 tccttttcta tttttgatga catcagtata tagctactcg aaagagtagt ctttttatta
     1441 ttctcgctta ttattgatat gaaaatttcg atttgacaaa aggtaccgaa taattttgta
     1501 atatgaatta ttatcaagaa gtaattatga tgataacata tggaaactag ttagtaataa
     1561 taacaggtga ttaaactttt gaacgacttg taattctata ttttttctt
//
LOCUS       DAJSBN010000164         1599 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJSBN010000164 DAJSBN010000000
VERSION     DAJSBN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.172"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(61..279)
                     /locus_tag="ONT86_002947"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="ONT86_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="ONT86_002948"
     CDS             complement(545..739)
                     /locus_tag="ONT86_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353652.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="ONT86_002949"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="ONT86_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          563 a          242 c          213 g          581 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatctta
//
LOCUS       DAJSBN010000165         1578 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJSBN010000165 DAJSBN010000000
VERSION     DAJSBN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.173"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            138..752
                     /locus_tag="ONT86_002950"
     CDS             138..752
                     /locus_tag="ONT86_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2353653.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(776..1126)
                     /locus_tag="ONT86_002951"
     CDS             complement(776..1126)
                     /locus_tag="ONT86_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR2353654.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1120..1383)
                     /locus_tag="ONT86_002952"
     CDS             complement(1120..1383)
                     /locus_tag="ONT86_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HCR2353655.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          521 a          286 c          245 g          526 t
ORIGIN      
        1 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
       61 cgcgacattg aaaaatctgg ttttctcgtg tatgattagg gtgtttttta aatagttgca
      121 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      181 aacaacggca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc attgagaaac
      241 aatctggcgc aacgattact catcgacctg tttttcaaga agcactggat ttcgtccgag
      301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      361 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
      421 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
      481 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
      541 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
      601 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
      661 gtgttgcgat cgctaagatt gcaaaagact acaatgttac gagacaaacc gtatatagaa
      721 taaaaaaaga cagcatggtc aatcacgaat agttgatcat gctgctgttt ctattttatt
      781 tgcttacttt ttgaaaaact gttctaactt tcatcaataa ctcctcaact aaactatcat
      841 gcgctttttc caaaaaaata cactctctgg cttcataatc aattgtcgtt agttgatcta
      901 ataatacttt tcctgttgtc ttcatttcat attctgagtt tatatttaca tatagcggat
      961 agtttctagt tgtactagaa attggagcaa ctgtaacaac gttactataa tccgcaataa
     1021 tatcatgact taaaacaata taaggtcttt tacctgtctg ttcgtgtcca gatcgaggtg
     1081 ctgtattcaa taatagaata tcgccttgtc taggttttct caccattttt cattacctac
     1141 tgcttcacct aaattttgaa tcgtcacatg atttctttca tcagtgtaat tttcaaataa
     1201 ctgttcaatt gttaattcat tttctatatt agccgcttcg ataattagcc gcttctgacc
     1261 gtttacttct tcttgtatta tcttcacttt gtcttctgta tgaattccaa gatattccat
     1321 aatatacttt ggcaatctaa ggccattact atttccccat ttttttatag gtaacatttc
     1381 catagtagac acctctttct gtatatacat agtataacat acataagaaa aaacgtatat
     1441 acttaattat ccttttctat taaattagat taacattcca tgaatccata aactatgtta
     1501 acatttatat gaaaatagtc gaaaggaaat cataatatgt ttaagaaaac aaaatcttta
     1561 gaatcaggta agaaaaaa
//
LOCUS       DAJSBN010000166         1573 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJSBN010000166 DAJSBN010000000
VERSION     DAJSBN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.174"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DAJSBN010000167         1509 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJSBN010000167 DAJSBN010000000
VERSION     DAJSBN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.175"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            101..868
                     /locus_tag="ONT86_002953"
     CDS             101..868
                     /locus_tag="ONT86_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2353656.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="ONT86_002954"
     CDS             1060..1458
                     /locus_tag="ONT86_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR2353657.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DAJSBN010000168         1495 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJSBN010000168 DAJSBN010000000
VERSION     DAJSBN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.177"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(522..878)
                     /locus_tag="ONT86_002955"
     CDS             complement(522..878)
                     /locus_tag="ONT86_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR2353658.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(892..1302)
                     /gene="spx"
                     /locus_tag="ONT86_002956"
     CDS             complement(892..1302)
                     /gene="spx"
                     /locus_tag="ONT86_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR2353659.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          468 a          265 c          213 g          549 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacaact cagttatctt atcatgtcat
      241 ttctaacatg tcaacaacta ttttatatgt tttttaaaat actattttat tgctttgcta
      301 cttaaaacaa cagaaatgaa tatatcatgt aaatttacac atgtcaatat atttttaact
      361 ctatatcccc acctagattt gaattttgac cacataaatt gagcatgtct ctatagtaga
      421 aaaataaagt caaaaaaaga acacataaca cgcttgatgc taagtgttcg actgttacaa
      481 ctgtatgaaa tgctcagtga cttgattaaa acagttaaag tctatttatt taagataagc
      541 tgttccatca actgctcatt gtatccaata aatagaactt catcgataat aatcaaagga
      601 cgtctgagga gattggtatg ttgtgtgcat aaaaaaatca gttgtttttt tcctacacag
      661 gaaaggtcaa tcgcaatgtt gtgaaaagcc tcagaccaag tcgcaaatag ctgttctgct
      721 tgcacttctt caaaactgca taattcttct gcttcttctg tacttagatc gcgttcttct
      781 aagtcaacaa attgataagg caattgatgg tgttctaacc attgtttcgc cttcagactt
      841 gttacacagt gcgctgatcc atagactttc acttccacga aggctccctc cctatgaatt
      901 taagatgtta ttgttttgta aatagatcag atttctggca acacatcgaa tttcacgagg
      961 gacaaatgac cggatctcat cttcattaaa tccgattagt attctttttt catcaatcaa
     1021 aataggtcgt ctcaataatg aaggatattc tttgaataag acgatcattt gctttaaagt
     1081 taattcgtta aaatctatgc ccagcttttc gtaaattttc gaccgagtgg aaataatatc
     1141 ttgaactcct ccttcagtcg ctgccaagat ctttttgatc tcactttctg ttaacggttc
     1201 ggcaaaaata ttcttctctt caaattcaag ttgattcgct tgcaaccatg ctcttgcttt
     1261 tctacaggaa gtacagctag gcgaagtata tacttttatc attttctcac tcatttttaa
     1321 ttataatcca attctacagt aatttctttt aaaataccaa ttattagcta ataatatata
     1381 aaaaaaaatc aaaataactg attatttacg tttgaataat gagaattttt ctatatattc
     1441 tctattttga taatagtatt ctcattttca aaataaataa gggaggtgtg acaaa
//
LOCUS       DAJSBN010000169         1485 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJSBN010000169 DAJSBN010000000
VERSION     DAJSBN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.178"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            287..694
                     /locus_tag="ONT86_002957"
     CDS             287..694
                     /locus_tag="ONT86_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353660.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="ONT86_002958"
     CDS             708..1109
                     /locus_tag="ONT86_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353661.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="ONT86_002959"
     CDS             1111..1482
                     /locus_tag="ONT86_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353662.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          570 a          183 c          259 g          473 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatga
//
LOCUS       DAJSBN010000170         1453 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJSBN010000170 DAJSBN010000000
VERSION     DAJSBN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.179"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..625)
                     /locus_tag="ONT86_002960"
     CDS             complement(<1..625)
                     /locus_tag="ONT86_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCR2353663.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ONT86_002961"
     CDS             complement(784..1050)
                     /locus_tag="ONT86_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2353664.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ONT86_002962"
     CDS             complement(1040..1396)
                     /locus_tag="ONT86_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR2353665.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          587 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aat
//
LOCUS       DAJSBN010000171         1437 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJSBN010000171 DAJSBN010000000
VERSION     DAJSBN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.180"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            76..690
                     /locus_tag="ONT86_002963"
                     /pseudo
     CDS             76..690
                     /locus_tag="ONT86_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1060..>1437)
                     /locus_tag="ONT86_002964"
     CDS             complement(1060..>1437)
                     /locus_tag="ONT86_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCR2353666.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          519 a          233 c          213 g          472 t
ORIGIN      
        1 taacattgaa aaatttagag ttctaacgta tgattagggt gtattataaa cgttatagaa
       61 aagtagggaa taaatttgaa aattgcttat attagagttt catccatcga tcaaaatgaa
      121 caacgtcaaa ttgaagaaat gaaaaagttt ggcgctgaaa aaatttttat cgaaaaacaa
      181 tctggagcga cgattagtca tcggccgatt ttccaggaag ctttagattt tgtcagagag
      241 caagatattt ttattgtaga agccattgat cgacttggac gcaattatga cgagattatc
      301 gattcagtga attatctaaa gaagaagaat gtgcgactta ttatcaccag tttaccgatc
      361 atggctgaag caattggaaa tcctctccta gataaattca tcaaagactt aatcatttaa
      421 atctcagcca tgattgctga acaagaacgt actgaatcga aacgacgcca ggcacaagga
      481 attaaaatcg ctaaagcaaa cggcgtctat aaaggtcgtc ctaagttata cagtgcagac
      541 accaaagatc ctcaacgtcg ccttgtctat aggagtatcg ttcaggatct tgagaatggg
      601 gttgctatct caaagattgc aacagactac aatgtgacta ggcaaacaat ttaccgaata
      661 aaaaaagaaa tcgaccaact aattgtatga tttctttttt gaatcacgta attattaatt
      721 agctttgtca tcaatacaga caatcatttt agattcatct agctaagttg attacattta
      781 tgttaaaaag aatttagtta acataaatat aattgatgca taaatcgtcc ttatttaaag
      841 gcatttaaca ttgacaactt agtataatgt atcttatgta aactaataag atttgaattt
      901 tattttatac acactcgata atagatcaat tttatggtcc cccgcattta cgattgcaca
      961 agagaaacat aaagtcgacc tattagcatt tcttttcaat tcaaacatta aaaaagttta
     1021 tacctctgct actaaaaaat gaaattatct atttaaaatt taaaacggta aatctgattc
     1081 atccaaattg gaaaatggat cagcctgtat tggagctttg tatttctcca taataacaga
     1141 tatctttttt tcatttttta aaaaatacct tttaaatttc ttatagtcct ctgaaatcaa
     1201 aatatagcgt gatggatact catcaaaaga agaatcgtat tcttccttta aaattcccga
     1261 tttacttaat ttttctagta catcaaatac tctatcactc aagtaactcc ttaaattgtt
     1321 atcactttcc cattgaacta cttttctata agttgaatta tcagttgaaa agatagaatt
     1381 ttcatccata aacgcatata ttagcagcaa tctttcatca ttgtcaaatt ctattgg
//
LOCUS       DAJSBN010000172         1435 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJSBN010000172 DAJSBN010000000
VERSION     DAJSBN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.181"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            105..1406
                     /locus_tag="ONT86_002965"
     CDS             105..1406
                     /locus_tag="ONT86_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2353667.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          473 a          287 c          264 g          411 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgtt
//
LOCUS       DAJSBN010000173         1417 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJSBN010000173 DAJSBN010000000
VERSION     DAJSBN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.182"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(150..1337)
                     /locus_tag="ONT86_002966"
     CDS             complement(150..1337)
                     /locus_tag="ONT86_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCR2353668.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAJSBN010000174         1395 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJSBN010000174 DAJSBN010000000
VERSION     DAJSBN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.183"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..237
                     /locus_tag="ONT86_002967"
     CDS             <1..237
                     /locus_tag="ONT86_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353669.1"
                     /translation="KRFGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERD
                     YPDESDDLFGVIPNKKSQMSESKFTSIFNDVNLR"
     gene            458..>1395
                     /locus_tag="ONT86_002968"
     CDS             458..>1395
                     /locus_tag="ONT86_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCR2353670.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKD"
BASE COUNT          501 a          199 c          321 g          374 t
ORIGIN      
        1 aagcgttttg ggtttgaaac ttttgctgat tttgagcggt ggttatcgtc aatttctgac
       61 gatcagcaac acgaaaaaac agtggataat gagtggcttg ctgatgaaaa atcagcgcca
      121 agcgaacgag attatcccga tgaatctgac gacttattcg gagtgatacc gaataagaaa
      181 agtcagatga gtgagagtaa atttacgtca atatttaatg acgtgaattt acgataagcg
      241 attattttgc gtgaagcaaa ataaagcata aaaggagttg atagcgtgat acgctttcgg
      301 ctcctttttt cgttgtcact tgtgacagcg aaaaccctcg gagagcgttt ggaattgtga
      361 catccatgtc acaattagga aaatgttact ctttgacaaa gagtaacaga aaggtttaca
      421 atctttttat tagcgacagc cgaaggaggg ttttaacttg gggtttagta tttcatttaa
      481 aaaaggaacg aagaatacaa gtatttctca taataatcgt gagttaactg atcgccagaa
      541 aaccaatgat tggcataagc acattgactt cacgaaatcg gatgaaaata tttatttaga
      601 gcagacgaat ctagcagata aatatgagga attgtttggc gaagtggtag aaaaatataa
      661 tgcgaaacaa aaaagagccg atagaaaaat cgaggactat ttagcgaaag tgcgcaagga
      721 taaaaagttg gaacctcaac gagaatttat tgtgcaaata ggcattttag atgatttcca
      781 gacaaatcga gcagatggtt cttctactgg catatcgaaa caacaagctg aacaaaatcg
      841 agtgattgcc aataaaattt tggttcagta ttacaaggag tttcaagaga gaaaccctaa
      901 tttggctgtt tataatgctg ttattcataa tgacgaaata agtccgcacc tgcacttgaa
      961 tattgtgccg gttgctgaag gttataaacg gggtgttcaa aagcagccga gctttaataa
     1021 agcactggtt caacaaggga tacacgctga taaagataat ggtcgagtgc ttttcaataa
     1081 ttttagaaat caagaagttg actctataga acgattaatg aatgattacg gttggaaaag
     1141 ggatattgtc ggtaccaaca aaattaaaga tattcacgaa tacaaagaaa tcatgagtga
     1201 aatttctgaa ttgcgccaaa cggctgtaaa agagcgtgag agcgtttctg atgagttgag
     1261 gtatatttat aggcaaagag aagaaaacga gcgtgataag gctctgatgg cttctgagag
     1321 agcgaagatt gaagaagaga agaaaaatat gctgaatgta aaaaaagaga tggcggtcat
     1381 tttccaaaaa gatat
//
LOCUS       DAJSBN010000175         1373 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJSBN010000175 DAJSBN010000000
VERSION     DAJSBN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.185"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            191..445
                     /locus_tag="ONT86_002969"
     CDS             191..445
                     /locus_tag="ONT86_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR2353671.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ONT86_002970"
     CDS             446..763
                     /locus_tag="ONT86_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR2353672.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          418 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat ata
//
LOCUS       DAJSBN010000176         1324 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJSBN010000176 DAJSBN010000000
VERSION     DAJSBN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.187"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            278..820
                     /locus_tag="ONT86_002971"
     CDS             278..820
                     /locus_tag="ONT86_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353673.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="ONT86_002972"
     CDS             976..>1324
                     /locus_tag="ONT86_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HCR2353674.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DAJSBN010000177         1314 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJSBN010000177 DAJSBN010000000
VERSION     DAJSBN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.188"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..848
                     /locus_tag="ONT86_002973"
     CDS             <1..848
                     /locus_tag="ONT86_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2353675.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          240 c          254 g          391 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaat
//
LOCUS       DAJSBN010000178         1280 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJSBN010000178 DAJSBN010000000
VERSION     DAJSBN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.189"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>1280
                     /locus_tag="ONT86_002974"
     CDS             <1..>1280
                     /locus_tag="ONT86_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR2353676.1"
                     /translation="EEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLP
                     LEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKA
                     IVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKA
                     VQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYH
                     HDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKN
                     TDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKS
                     KLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTS
                     GIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVL"
BASE COUNT          426 a          229 c          277 g          348 t
ORIGIN      
        1 tgaagaaatt tctgtaagtg ttccagaagc agctgccacc attattccag taaaaggaca
       61 tcaacgagtt gtaggaacga aagcagacaa aatcaaacac ttacctataa cagagaaaga
      121 acatctcctt cctttggaag aacaattttg tgaacattgc ggttcacaga tgaaagatat
      181 cggacggaca aaagtacgag aagaaattcg tttccatcag gctatgttgg attgtctcac
      241 ccattatcaa catacctatt gttgtaaaag ctgcgaaaaa gaaggacttt cttcttttaa
      301 aaaagcgatc gttcctaaac ctttgatttc taatagctta ggttccaata gtttagtggc
      361 ggaaacgatc cggatgaaat ttggtcagaa agttcctgcc tatcggcaag agaactattg
      421 gaaacaaaca catgggttgg atatttcgag agataatatc accaattggc atattaaagc
      481 agtgcaaaac gcgttagacc ctttaggaga acgattaaga gtctatctta atcaagaaga
      541 gattctacac ggggatgaaa caagttatcg tgtgattgaa agtgccaaaa cagataccta
      601 ttactggcag ttttgtacag gaaaagacag tcagcatccc atcgtttatt atcaccacga
      661 cgaaagtcgt gctggagatg ttccgaaaac ttttctcaag gagtttacag ggtatttaca
      721 ctgtgacggc tatagcgggt ataatgccgt ggaaagtgta cgacttgtct attgtttcgc
      781 gcatgttcgg cggaaatttt tcgaagcaat tccaaaagga aagaaaaaca cggatattcc
      841 cgcagctcaa gcagtcaaac aattggataa atggtttgtc ctagaaaaga aatggaaaga
      901 cttttctcct gagaagcgat taagctgtcg gcaacaagaa ctgcgtccat tatttattgc
      961 tttttatgag tggatggcaa cgatagatcc tgttgcaaaa tccaagttgg atgcagccgt
     1021 tcaatatgcg tgtaaactac gaagtggttt tgaacctatt tttgaagatg gtcgtttaga
     1081 actcacaaat aatcgtgcgg agcgaaatat caaagagctg gtgataggac ggaaaaattg
     1141 gttgcattcc acaagtcttg aaggagcacg aacttcagga atcattctga gtgtatataa
     1201 aacagcagaa cttaacggat tgaatcctgt aaagtatcta gagtttctat ttgataagat
     1261 accaaatctt cccgtacttt
//
LOCUS       DAJSBN010000179         1271 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJSBN010000179 DAJSBN010000000
VERSION     DAJSBN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.190"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..73)
                     /locus_tag="ONT86_002975"
     CDS             complement(<1..73)
                     /locus_tag="ONT86_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2353677.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDT"
     gene            complement(57..1271)
                     /gene="aph(2'')-I"
                     /locus_tag="ONT86_002976"
                     /pseudo
     CDS             complement(57..1271)
                     /gene="aph(2'')-I"
                     /locus_tag="ONT86_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
BASE COUNT          429 a          220 c           99 g          523 t
ORIGIN      
        1 tggtatcatt aagaaatcct tttaaattat caatatcaaa aaccatatta tcatcaataa
       61 acaatagttt caataggata atattcttca actatatctt gatattcttt tgctttctca
      121 atatctatat ttccatacat tcttaatata tcttctccaa aatttgttcc tatttcttct
      181 tcactatctt caagtaagta tataaaatca caatattcat ctataattcc agaatctcca
      241 aaatcaatta ttccagttaa tctattattg ccatctaaca atagatgatt acaactaaaa
      301 tcattatggc ataaacactt tttaccctca aaaactgttg ttgcatttag tctttccata
      361 aaactttcta tataatcttt ttctatatca gttaaatcat tataaatagt ttcacgcaac
      421 aatatatact cttctaatac attttgttta ttatcaatag tacattcact aatatctgta
      481 taatctaaac cgtgcatttg tcttaaaaaa ctggcaatat ctcgttttaa caaattttgt
      541 tcttcttctg acatagtaga ataaatttct ggtgttaaaa aagttccttt aatttcttta
      601 taacctagta tagataattc atcactaata tacgaatatt caatattagg aatttttaca
      661 ttagtttcta aatttgtatt taaaaaatta tatattgctt tttcttttgc ataacctttt
      721 ttcttattag tactaaattt tgttttaaaa atgtattcat tattaactaa atatgccaca
      781 ctatcataac cactaccgat tatttcaata ctatctactt tgaaattatc aaagtaatgc
      841 tcaattaaat atttcattgc cttaacattt gtggcattat catcatatct atattccatt
      901 aaataacaat cttctttttt gccctcgtgt aattcatgtt ctggcaaatc ttcaataatt
      961 ctaaaaccag atttttggta tgcccttatt gctcttggat tatttttatg agggtctaaa
     1021 ataactgcat tagcatttct ttcttttttc aaaaattcaa aaatcaattt aatatatctt
     1081 gtaccaattc ctttactcca ataatttggc tctcctataa attgatccat accatagact
     1141 atctcatcag tttttggata atgataatca gtatataact catcatacat tttatatatt
     1201 tgtccatatc caataggaac attgttatat tcaataatta ctctaaaaac ttcatcttcc
     1261 caaggctctg t
//
LOCUS       DAJSBN010000180         1267 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJSBN010000180 DAJSBN010000000
VERSION     DAJSBN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.191"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1101
                     /locus_tag="ONT86_002977"
     CDS             22..1101
                     /locus_tag="ONT86_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2353678.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          378 a          301 c          310 g          278 t
ORIGIN      
        1 aatagacaag gatgaattca catgacgcaa acccatgata acacagtttc acgcaaggga
       61 aaacatcttt cctactcaga acgttgccaa atcgctatcc tgaaaaagga aggctattcc
      121 aaccgtcagg ttgccaaagt gctgggacgt gttccgcaaa ccataaacaa tgagattgat
      181 cgcggaacca tcacccagtt aaaacgccaa acgcaaaatg gaaaagtgta tgacacctac
      241 tattctgttt atgacgctga tgcaggacag gccttctatg aaaagcaacg actgaactgt
      301 ggcagacgcc ccaaatgggc gaacacaaat gccttcattg attgggcaga tgacaaaatg
      361 ttaaatgaaa aatggtcgcc ggacgtcgtg gtcggatttg ccagaaaaca cgagctgttc
      421 gattgtgcca tcattcctgg cacaaccgct ctttatcaat ggattgaccg cggaatcatg
      481 cgaacaaaga acatggactt gcttgaaaaa ctgtcccgta aaatcaaagc ggtccgccca
      541 ggacaccgtc ccaacaagcg tgttctcggg acgtcaattg aagaacgccc taaagaagtt
      601 gaaacacggg aaacgttcgg tcattgggaa atcgacacgg tagtgggaaa caagaccaag
      661 tcggatgccg tgctgctgac gctggctgaa cggcagacac gctttgaagt catcctaaag
      721 ttgaacggga aggacaccga atcggtggac caagcggttc aacagctaag agaacgggct
      781 ggcgctaact tcgatcaatt atttaagacc gttacatccg acaatggctc ggagttttct
      841 gggctacacg atgctctgca agatgtagct gacgtctatt tcagccaccc ctatgcttct
      901 tgggagcgtg ggacgagcga aaaccagcac aaattaattc gccgtttcgt ccccaaggga
      961 agcccgatta gtgaggtgag cgagaaccag attttgcgga tccagcgttg gatgaacgat
     1021 tacccgcgga aaattttggg ttatgcgaca ccacatgata cattcgtcca ggcattcaag
     1081 caagaacggt tggtggcttg acccattatt ccttgataac gatcctcact cgggacatgg
     1141 cttcatgtct ggaaagctgg cctggggcca gaatcgtctg aagactatca tacctcagtt
     1201 cattatcaag gcctcactga tttagaataa cgaggcagtt acggtatcaa acaaatgagt
     1261 tacctgt
//
LOCUS       DAJSBN010000181         1233 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJSBN010000181 DAJSBN010000000
VERSION     DAJSBN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.192"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            358..444
                     /locus_tag="ONT86_002978"
     CDS             358..444
                     /locus_tag="ONT86_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCR2353679.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(565..>1233)
                     /locus_tag="ONT86_002979"
     CDS             complement(565..>1233)
                     /locus_tag="ONT86_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353680.1"
                     /translation="LLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPGVIFTLS
                     IVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCYEEMND
                     IFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEACYNL
                     FSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEWLDQ
                     QQQN"
BASE COUNT          348 a          244 c          205 g          436 t
ORIGIN      
        1 taaacaggtt cgcgcatttt tcaaaactgc caatccaaaa ccttgcttcc tacggtcgcc
       61 aaaaaggtgt gatcgctacg ctctcacatt gtactgcgca gtcgcctaaa ggctcctttg
      121 ctaaaaacat gttggtaaag aggcctttga gcggttctga atgcttatct aagccgtttt
      181 aagacagtct tggataaaaa tccttatcta gctttaaagc gccttacagg cggtcagggc
      241 aagccctaac aaccctaaaa taagcgatac ttaaaaacac cacacaagtt taatttttta
      301 agatataata acttgtcgcc tatccgacag tcttgcgact ggaaggaggt gtatctaatg
      361 tttgaattgt tcatttgccc ccttcttata gaagtttgtg tctctttatt cagttattgg
      421 ttgaataacc gaaacaagaa gtaaaatcaa taaaggcgac atcaacctga aaaaaagccc
      481 cagagaatcc gacctctctg gggctttcgt gtatctaatg tttgatcatt tgcatgttta
      541 gtttaacatg attgccgatc aaattcaatt ttgctgttgc tgatctaacc attccttgac
      601 actttttaga taatttaatg ccgtttcttt tttcgttgga ttttgtatgt tcgttgcttg
      661 ctgaagcagt gctttataac gttcgtaggt aactgattgt tgccgttctt ttcgttcctg
      721 ttcactagtt gaaaaaggga ttgtatcact gaataaatta taacaagctt cttctgtctg
      781 cgtatcttta aaatattgca cgtctttttt ggttcgctca tagttgtctt gaacctcttt
      841 ttgctgtttt tctggcaagt tttgataaag acgagtgagt ttttccatgt ttttatccgt
      901 caaacgtttt tcttgattca caaaaatatc gttcatttcc tcataacaag tggtaaactc
      961 ttccattttt tgtgttaaca cttgctcctt tttcttttta tcacatgctg cttcatactt
     1021 ttgagtgata tcccctaata aaatacttct tgaaatacgt ttatttccct ctaaaacgcc
     1081 gaataaacca cttaaaacga ttgataatgt aaatataacc cctggcaata aaaacctctt
     1141 aaatcgaatt ttagagcctt ttaatgaata tcttgtttga taagccttat agctaagcca
     1201 aataattcct aaaatactgg caaaaatgag tag
//
LOCUS       DAJSBN010000182         1214 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJSBN010000182 DAJSBN010000000
VERSION     DAJSBN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.193"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            70..1026
                     /locus_tag="ONT86_002980"
     CDS             70..1026
                     /locus_tag="ONT86_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353681.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1080..>1214)
                     /locus_tag="ONT86_002981"
     CDS             complement(1080..>1214)
                     /locus_tag="ONT86_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2353682.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          427 a          179 c          207 g          401 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga
     1201 ttgacttggt tgaa
//
LOCUS       DAJSBN010000183         1193 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJSBN010000183 DAJSBN010000000
VERSION     DAJSBN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.194"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1076
                     /locus_tag="ONT86_002982"
     CDS             <1..1076
                     /locus_tag="ONT86_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR2353683.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAJSBN010000184         1187 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJSBN010000184 DAJSBN010000000
VERSION     DAJSBN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.195"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..336)
                     /locus_tag="ONT86_002983"
     CDS             complement(<1..336)
                     /locus_tag="ONT86_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353684.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLT"
     gene            complement(329..562)
                     /locus_tag="ONT86_002984"
     CDS             complement(329..562)
                     /locus_tag="ONT86_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353685.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(559..>1187)
                     /locus_tag="ONT86_002985"
     CDS             complement(559..>1187)
                     /locus_tag="ONT86_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2353686.1"
                     /translation="SQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQ
                     LRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKK
                     YRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT          346 a          263 c          165 g          413 t
ORIGIN      
        1 ggtcaatttt tgttgatcac gaatcatttc tgttaattca gaaatatgtt tgtccttctc
       61 agctaattga tcacgcaaaa actcattctc tttttctaaa ggaaaatcac tgcctgtgtt
      121 attgaccggt aattgaccag tcaattgatc actttttgac cgataaattg accgaacaac
      181 accaatcaac tctttatcta gcatgataat gccattttct ttatactgag caaggtcaaa
      241 ccctgaattg accagttgat tgcgcactgt atttttcgat acacccaatt cgtcagcaag
      301 attttttatg gtcatctttt ctgacaaatc agtcattagc ttctccacta ctttcagcta
      361 ccgcataaat atatttaacg gcgtgcttag ggataataat cggagagtca attttattat
      421 ttttcaaaat caattcatat tgcgttacat caagtaactt accggacatt tttttgctat
      481 ccaaaaaaac aaccacaacg taatcacctg ttaaatttac caaccaattt ttcttaccaa
      541 aatacttttg tttttctgtc atttttcttc ctcctctgtt acgtctagcc ctagaagcca
      601 tgtgtctaat tcttttttta aatcgtctgc cgattccgca aaggtttctc gcaaatgatt
      661 cgtcaattca tcattttgac tttcttgctt caacaaatcc ctgtattttt tctttgtatt
      721 cttagctaga tttccccact cagaatgatc ataaagcaaa cctttcacca taatccgttc
      781 agtaacttta gtatttggcg gaaaataatg tggactagca aagctatcca aactttcatg
      841 aaccatctca tgccaatcag ttgcttttcc ccttcgtaac tgcaattcta gtcgccacca
      901 tgtcgttatt tccggcggaa caattttacg ttttctttct tgttccagtt ttttattata
      961 catccgaatt tgtcgttcac tggcacgact accccaatat gccgtttcta acttaccaga
     1021 acgcccataa attggtttaa aagacacagg atcaactacc cgatattgtg taataaactc
     1081 atcaggtaca tcgataatgt cgcaagcaat atccgcacgt gaaaagtgag gttcgataaa
     1141 caaatcatga ataaaatttt tcatggaact agctaagaat tgactaa
//
LOCUS       DAJSBN010000185         1145 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJSBN010000185 DAJSBN010000000
VERSION     DAJSBN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.196"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            55..>1145
                     /locus_tag="ONT86_002986"
     CDS             55..>1145
                     /locus_tag="ONT86_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353687.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTG"
BASE COUNT          368 a          114 c          206 g          457 t
ORIGIN      
        1 tatcagttta ttaaactcgt atatagaaca aatatatgta gacggggcga aacgatgagt
       61 gtaaaaaaaa tgctatttat ggtaagtaca ataattgtat taacatcccg attttgggga
      121 attaatacta gtttatctat gcgatatttt attatggcaa tatgggattt atattttatg
      181 ttaagctatt tttcttatcg tgagaaaaca tataaaaaat acgttatcaa gaataacttt
      241 tggttgacta taatgccgtt tgtgatattt tctatctata ctctaataat atggggaatt
      301 ggttcaaatg cagaatttga aaattttata aagttatgtt ccacgttgct ttatttaatt
      361 cttgcatatg gttacgcatg tacagcattt tacttattta aggcagaagg aattgatttt
      421 attttttggt caggtgttat ttcatatatg agtggttcag ttttttattt aattgtatct
      481 tatggtttaa atggtttaat ttcttatata aaaagtctta taacaggtga aacaaatact
      541 gcaaattttg ctatggaggt acatgattta acttttgcga tgggggtttt tttcctttat
      601 tacgtatttt ttgaaaataa aaatacaaaa aaacataaga taaaaattat cgtatcttta
      661 attttaattt tcatgggatt gaagagaatt gaactgttag ctttaagtgg tgcaattcta
      721 gtgtattatg ttttattaaa gtggggaaaa aagattcaaa tccgtgcatt agtgtgtact
      781 atttgtgcaa ctgttatttc gttagtgttt atttacatta tagatacagg aattttggca
      841 ttgcttatgt caaatttggg cattacagat gacggtgcga ggctttcatt ttatctatat
      901 tcatctaaat tttatgaact tggtttagga tatgtaggaa caggatggac atggttttca
      961 aaatactttt ttaatcttta tttatcgcac tttaggttaa atggtggaag aattgcagca
     1021 agcctacaca gtgatatatt aactatttat atagaagttg gatgtatagt ttttattata
     1081 tggatgtttt ggttattttt tgtgaaaccg ataagactaa ctaaaaaatt tggattgaaa
     1141 acagg
//
LOCUS       DAJSBN010000186         1143 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJSBN010000186 DAJSBN010000000
VERSION     DAJSBN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.197"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ONT86_002987"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ONT86_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR2353688.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DAJSBN010000187         1121 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJSBN010000187 DAJSBN010000000
VERSION     DAJSBN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.198"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            292..>1121
                     /locus_tag="ONT86_002988"
     CDS             292..>1121
                     /locus_tag="ONT86_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2353689.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          395 a          155 c          189 g          382 t
ORIGIN      
        1 taataatata ctaccatttg ttggtagtat aatttaaagg taataagtta ttcttttatt
       61 actatgtttg ggttttatag caaagaaagt ttaataagag tttggaccta gcaaacaata
      121 ttaatgaggt cattaaatct tattatcttt agtgatataa cgaaatatct acacagacaa
      181 aatgatagcg taaaatattt tgtgtaaata gatatttagg gtagaaaaaa agaaagtcca
      241 ttctgtaaaa ttaaagttac cacaaccgaa attttatagg aggactttct catgcccaat
      301 tttactacag aaattatgga aacactaatc aataaaggtg atttagatga attatttcga
      361 tgtcatttag aattagctgt taattcatta ttgcaggctg aattaacagc ttttcttgat
      421 tatgaaaaat atgatcgtgc tgggttcaat tcaggtaatt cccgtaatgg gaattattca
      481 cgttcattca gaacagagta tggagaatta aatttggtaa ttcctagaga tagaaatggt
      541 aagttttctc aacaaacttt acctgcttat aaaagaacca atgactcttt agaaaccact
      601 attattcagc tatttcaaaa aggaattact atgtctgaaa tctctgaatt gattgaaaaa
      661 atgtacgggc atcactatac gccacaaacc atttcaaata tgactaaaat agtttctgaa
      721 gatattattg cctttaaaga aaggtcctta gaatccagat actctgtcat ttttatggat
      781 gctactcaca ttcctttaaa aagacaaact gtttcaaaag aagcagttta tattgtaatt
      841 ggcattcgat tagatggaac caaagaggtt cttgggttta gtcttgctcc aactgaatct
      901 gcttatgttt ggaaagagat tcttcaagac ctaaaagacc gtggtttaaa agaggtttta
      961 ttagtcgtaa ctgatggttt aagtggcatt aacgatagta ttcatagtgt ttatccaaat
     1021 gctcaatttc aacaatgttg tgtccatata tctagaaata tcgctcataa ggttcgtgtt
     1081 agtgatcgac aagaaatctg tagcgatttc aaattagttt a
//
LOCUS       DAJSBN010000188         1118 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJSBN010000188 DAJSBN010000000
VERSION     DAJSBN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.199"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            82..>1118
                     /locus_tag="ONT86_002989"
     CDS             82..>1118
                     /locus_tag="ONT86_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCR2353690.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFR"
BASE COUNT          374 a          212 c          218 g          314 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgaga
//
LOCUS       DAJSBN010000189         1075 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJSBN010000189 DAJSBN010000000
VERSION     DAJSBN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.200"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            82..>1075
                     /locus_tag="ONT86_002990"
     CDS             82..>1075
                     /locus_tag="ONT86_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HCR2353691.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLK"
BASE COUNT          362 a          196 c          209 g          308 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaagg
//
LOCUS       DAJSBN010000190         1051 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJSBN010000190 DAJSBN010000000
VERSION     DAJSBN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.201"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ONT86_002991"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ONT86_002991"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCR2353692.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DAJSBN010000191          997 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJSBN010000191 DAJSBN010000000
VERSION     DAJSBN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.202"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(26..913)
                     /locus_tag="ONT86_002992"
     CDS             complement(26..913)
                     /locus_tag="ONT86_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR2353693.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DAJSBN010000192          994 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJSBN010000192 DAJSBN010000000
VERSION     DAJSBN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.203"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..>994)
                     /locus_tag="ONT86_002993"
     rRNA            complement(<1..>994)
                     /locus_tag="ONT86_002993"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAJSBN010000193          988 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJSBN010000193 DAJSBN010000000
VERSION     DAJSBN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.204"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..704
                     /locus_tag="ONT86_002994"
     CDS             <1..704
                     /locus_tag="ONT86_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2353694.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          335 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataa
//
LOCUS       DAJSBN010000194          959 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJSBN010000194 DAJSBN010000000
VERSION     DAJSBN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.205"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(107..511)
                     /locus_tag="ONT86_002995"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ONT86_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="ONT86_002996"
     CDS             complement(570..>959)
                     /locus_tag="ONT86_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2353695.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAJSBN010000195          856 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJSBN010000195 DAJSBN010000000
VERSION     DAJSBN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.207"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            83..>856
                     /locus_tag="ONT86_002997"
     CDS             83..>856
                     /locus_tag="ONT86_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2353696.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNFHLDSTHIKANA
                     NKHNFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEQEKQFAYSAHTSCDDNGFILSTIITPGNIHDS"
BASE COUNT          285 a          158 c          165 g          248 t
ORIGIN      
        1 ataaaatgcc agactgtaga caaagtccat tttatggatg atgtctacag tcttttcttt
       61 tataatagat ttatgaaaac gaatgcaagg agtgatagct atgatgtcta aaaacaatac
      121 gaatggtaga aatcaatttg cgatgcttac gattgatgat ctagttcctc aagatcatct
      181 cgttcgaaag attgatgccg cgcttgattt tgaatttatt tatccaatcg tcgaagccac
      241 ttactccgat ttaggtcgcc caagtatcga cccagtgatt ctgatcaaat tggtgtttat
      301 tcagtattta ttcggtatcc gttccatgcg acaaaccatt aaggaagtcg ataccaatgt
      361 cgcttaccgc tggtttttag gctactcctt cgaagagaaa atcccccatt tctcaacttt
      421 tggaaagaat tatgttcgcc gatttagaga aacgacggtg tttgaggata tttttgccta
      481 tattttagaa caagcagtta aagcaggttt tgtaacagag gataattttc atctagattc
      541 tacccacatc aaagctaacg ccaataaaca taattttacg aaagaaatga cccatgatga
      601 agcgaaagcc tatcaggatg aattagaaga tgagatcaat cgtcaacgaa tagaggctgg
      661 aaaacgccct tttactttgg acttagaaaa ggaagtgaag ctgaaagaaa gaaaaataag
      721 taaagctgat ccggaaagcg gctactacgt caaaggcgaa caagaaaaac aatttgctta
      781 ttctgcccat acttcatgcg acgacaatgg atttattctt agcacaatca tcactcctgg
      841 aaatattcat gacagt
//
LOCUS       DAJSBN010000196          844 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJSBN010000196 DAJSBN010000000
VERSION     DAJSBN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.208"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            15..>844
                     /locus_tag="ONT86_002998"
     CDS             15..>844
                     /locus_tag="ONT86_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HCR2353697.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          290 a          123 c          155 g          276 t
ORIGIN      
        1 aagaggactt ccatatgaac gattttacta cagaaattat ggaaatacta atcaataaag
       61 gtgatttgga tgatttgttt cgtcgtcacc tagaattggc tattaacgct ttattacaag
      121 ctgaattaac ggtatttctt gactatgaaa agtatgatag agctggattt aattcaggta
      181 attcccgtaa tgggaattat tcgcgctctt ttaaaacaga gtacggtgaa ttgaatttag
      241 taatacctag agataggaat ggagactttt ctcaacaaac tttacctgct tataaaagaa
      301 ccaatgactc tttagaaacc actattattc agctagttca aaaaggaatt actatgtccg
      361 aaatctctga tctaatcgaa aaaatgtatg gtcattatta caaaccacaa actatttcaa
      421 atatgagtaa aattgtgtct gaagatgttt cggcatttaa agaaagaact ttagaagcta
      481 aatactcagt catttttatg gatgctactc atattccctt aaaaagacaa accgtatcaa
      541 aagaagctgt ctatatagta attggtatca ggttggatgg aactaaagag gttctaggat
      601 ttactatagc tccaactgaa tctgcttatg tttggaaaga gattcttcaa gatctaaaag
      661 accgtggttt agaagaggtt ttgttagtcg taacagatgg tttaagtggt attgacgata
      721 gtattcatag tgtttatcca aatgctcaat ttcaacaatg ttgtgtccat atatctagaa
      781 atatcgctca taaggttcgt gttagtgatc gacaagaaat ctgtagcgat ttcaaattag
      841 ttta
//
LOCUS       DAJSBN010000197          816 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJSBN010000197 DAJSBN010000000
VERSION     DAJSBN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.209"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            136..309
                     /locus_tag="ONT86_002999"
     CDS             136..309
                     /locus_tag="ONT86_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353698.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          274 a          131 c          103 g          308 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaa
//
LOCUS       DAJSBN010000198          811 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJSBN010000198 DAJSBN010000000
VERSION     DAJSBN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.210"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..672
                     /locus_tag="ONT86_003000"
     CDS             <1..672
                     /locus_tag="ONT86_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2353699.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAJSBN010000199          766 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJSBN010000199 DAJSBN010000000
VERSION     DAJSBN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.211"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(109..>766)
                     /locus_tag="ONT86_003001"
     CDS             complement(109..>766)
                     /locus_tag="ONT86_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HCR2353700.1"
                     /translation="IGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQP
                     LEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTD
                     VKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQ
                     FETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA
                     "
BASE COUNT          213 a          175 c          146 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgatt
//
LOCUS       DAJSBN010000200          753 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJSBN010000200 DAJSBN010000000
VERSION     DAJSBN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.212"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..734
                     /locus_tag="ONT86_003002"
     CDS             <1..734
                     /locus_tag="ONT86_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCR2353701.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQ
                     YQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIR
                     WYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          321 a           97 c          127 g          208 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg attctacatt cagatcaagg atggcagtat cagatgccac
      481 aatatcaaaa aaaattaaaa gagaataaca ttattcaaag catgtctaga aaaggaaatt
      541 gcttagataa ttctgtaata gaaaattttt ttggtgtgct taaatcagag tttttttatc
      601 gagaaaaatt ccgatcaatt gagatatttc aaagtaaatt aaatgagtat attaggtggt
      661 ataataacaa aaggataaaa ctaaagttaa atggactatc tccagtagaa tatcggaaac
      721 agtccattaa atagttcaac ttttggggtt cag
//
LOCUS       DAJSBN010000201          733 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJSBN010000201 DAJSBN010000000
VERSION     DAJSBN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.213"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>733
                     /locus_tag="ONT86_003003"
     rRNA            <1..>733
                     /locus_tag="ONT86_003003"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DAJSBN010000202          730 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJSBN010000202 DAJSBN010000000
VERSION     DAJSBN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.214"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            111..>730
                     /locus_tag="ONT86_003004"
     CDS             111..>730
                     /locus_tag="ONT86_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR2353702.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPVTYSITNPGVHDVKVLETLSEEANLPNILGDKGYI"
BASE COUNT          257 a          128 c          126 g          219 t
ORIGIN      
        1 cccgtggtgc tagttaattt tttcctagag gaaaatgtct aaacatgttg gtaaatcaac
       61 aaaaaaacgc atatagtagt ctcaaaactc ccaagaaatg aggactacat atgcgttcat
      121 caaaatatac tgaacattat acagatactt ttataacatt caaggaaatt atgagaactg
      181 tttctaacat ataccacaat tgtgtaccag ataagattaa aaatcgaaga aatactgatc
      241 aactgaagca acgcgataca gtgattattg cttgtgttat atggggcata attaatggct
      301 atactagcca aagagccacg tatagagcgg tttgttccgt cttatttcct aatggtgact
      361 ttcctagtag gagtcgattc actcgcttaa gttcaaactt agcttacact atcaagatta
      421 ttcgatactt ttttatcaag aagctcacca aaggtgaact agtcggaatt atagatagtt
      481 ttcctagtcc attatgtaaa ccagttagaa atagacaagc aaaactattg aatcaaatag
      541 ccaaagttgg ctataactca acaaaaaaat cttattttta tggtcttaaa attcatatga
      601 tcgttactaa gactggcttt ccggttactt actcaatcac gaatccaggt gtccatgacg
      661 tgaaagtgtt agagacttta tcggaagaag cgaatcttcc caatatccta ggggacaaag
      721 gatatattag
//
LOCUS       DAJSBN010000203          729 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJSBN010000203 DAJSBN010000000
VERSION     DAJSBN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.215"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..729
                     /locus_tag="ONT86_003005"
     rRNA            <1..729
                     /locus_tag="ONT86_003005"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          180 a          162 c          229 g          158 t
ORIGIN      
        1 tgggatacta cccctgcgtt atggccactc taacccgcac cactaatcgt ggtgggagac
       61 agtgtcagat gggcagtttg actggggcgg tcgcctccta aaaggtaacg gaggcgccca
      121 aaggttccct cagaatggtt ggaaatcatt cgaagagtgt aaaggcagaa gggagcttga
      181 ctgcgagacc aacaagtcga gcagggacga aagtcgggct tagtgatccg gtggttccgc
      241 atggaagggc catcgctcaa cggataaaag ctaccctggg gataacaggc ttatctcccc
      301 caagagtcca catcgacggg gaggtttggc acctcgatgt cggctcgtcg catcctgggg
      361 ctgtagtcgg tcccaagggt tgggctgttc gcccattaaa gcggcacgcg agctgggttc
      421 agaacgtcgt gagacagttc ggtccctatc cgtcgcgggc gttggaaatt tgagaggagc
      481 tgtccttagt acgagaggac cgggatggac ttaccgctgg tgtaccagtt gttctgccaa
      541 gggcattgct gggtagctat gtagggaagg gataaacgct gaaagcatct aagtgtgaag
      601 cccacctcaa gatgagattt cccatttctt taagaaagta agatccctga gagatgatca
      661 ggtagatagg tcaggagtgg aagtacagtg atgtatggag cggactgata ctaatcgatc
      721 gaggactta
//
LOCUS       DAJSBN010000204          712 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJSBN010000204 DAJSBN010000000
VERSION     DAJSBN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.216"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..446)
                     /locus_tag="ONT86_003006"
     CDS             complement(<1..446)
                     /locus_tag="ONT86_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2353703.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DAJSBN010000205          703 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJSBN010000205 DAJSBN010000000
VERSION     DAJSBN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.217"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            279..677
                     /gene="spxA"
                     /locus_tag="ONT86_003007"
     CDS             279..677
                     /gene="spxA"
                     /locus_tag="ONT86_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR2353704.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          251 a          100 c          131 g          221 t
ORIGIN      
        1 tagcttttga gataaaataa tagtacagtt ttatacgctg tttcatatgt ttagaaaatt
       61 tgaattagat ttgaaaactc taatttttaa atgataaaaa aagcgagaat gctggtttta
      121 ctgtatatta accttagata actttcattt ttttaaaaaa attatgaaaa aaaggtagct
      181 atttaaaagt tgattatgta taatagaaac agatgggaga ttattatgat cttctgttac
      241 aaaaaagtga ttcttatcac gaaaaggagt ggtgcacgtt gttaacatta tatacttccc
      301 caagttgtac ttcctgcaga aaggctcgtg cgtggttgca agaacatcag attccatttg
      361 ttgaacgtaa tattttttca gaaccattga acagttctga attaaaagcg attttacaaa
      421 tgacagaaga cggaacagaa gaaatcatct cgacacgttc caaagtattc caaaaactga
      481 acatggattt agatgatctg ccgttacaag aattattgga acttgtgcaa aacaatcctg
      541 gattgcttcg ccgtccaatc atgattgatg ataaacgtct gcaagttggt tttaatgaag
      601 atgaaattcg tcggttccta ccgcgcgatg tacgacaatt agaattacgt caagcacaat
      661 tgatggctgg attatagaag gataatacga aacttggaaa aga
//
LOCUS       DAJSBN010000206          696 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJSBN010000206 DAJSBN010000000
VERSION     DAJSBN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.218"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(54..629)
                     /locus_tag="ONT86_003008"
     CDS             complement(54..629)
                     /locus_tag="ONT86_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2353705.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAJSBN010000207          649 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJSBN010000207 DAJSBN010000000
VERSION     DAJSBN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.219"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>649
                     /locus_tag="ONT86_003009"
     CDS             <1..>649
                     /locus_tag="ONT86_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2353706.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DAJSBN010000208          635 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJSBN010000208 DAJSBN010000000
VERSION     DAJSBN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.220"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..270)
                     /locus_tag="ONT86_003010"
     CDS             complement(<1..270)
                     /locus_tag="ONT86_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353707.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="ONT86_003011"
     CDS             complement(276..>635)
                     /locus_tag="ONT86_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR2353708.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DAJSBN010000209          583 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJSBN010000209 DAJSBN010000000
VERSION     DAJSBN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.221"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>583
                     /locus_tag="ONT86_003012"
     rRNA            <1..>583
                     /locus_tag="ONT86_003012"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          125 c          169 g          117 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gta
//
LOCUS       DAJSBN010000210          578 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJSBN010000210 DAJSBN010000000
VERSION     DAJSBN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.222"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          164 a          129 c           98 g          187 t
ORIGIN      
        1 agggctgtta atttttccca ctcattcacg attgttcttt ttttactcat attcagttcc
       61 tcctcaaaga aaaaagagat ttttggaaag aatttccaga gttcacgtaa ctctaaaaat
      121 tcataaccat caatctctag tcttgaagaa acctaaaaat attcgtataa tgaatttgag
      181 ttacggaact agtgtggtta gtttccgtaa aggtcgagaa cgttggtagc gttgctcgac
      241 ttattttttt gctctttttc cagttcattt gtttgctaac tcaataatag cacttctctt
      301 tagctatgca aataccaaaa aaataaaacc gcaccctttg aaaagggcgc ccccaaactt
      361 cgctaacgct aagttggcca aaaaatggac ttgccatttt ttagcaaatg tcagcttcag
      421 gggctagtgg tttagccgtc aactccttat cctcggcaca aaccgcctcc ggtaccgttg
      481 actgcacatc gtggctacca ctcaatctca ttctcagaac gctctaattt atgctgtgag
      541 acgttttgac gataaaataa ggttttctcc tgctctaa
//
LOCUS       DAJSBN010000211          561 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJSBN010000211 DAJSBN010000000
VERSION     DAJSBN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.223"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..156
                     /locus_tag="ONT86_003013"
     CDS             <1..156
                     /locus_tag="ONT86_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR2353709.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>561
                     /locus_tag="ONT86_003014"
     CDS             175..>561
                     /locus_tag="ONT86_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2353710.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNI"
BASE COUNT          248 a           46 c           98 g          169 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat t
//
LOCUS       DAJSBN010000212          526 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJSBN010000212 DAJSBN010000000
VERSION     DAJSBN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.224"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ONT86_003015"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ONT86_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR2353711.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="ONT86_003016"
     CDS             complement(349..>526)
                     /locus_tag="ONT86_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353712.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAJSBN010000213          524 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJSBN010000213 DAJSBN010000000
VERSION     DAJSBN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.225"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..524
                     /locus_tag="ONT86_003017"
                     /pseudo
     CDS             1..524
                     /locus_tag="ONT86_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          218 a           91 c           95 g          120 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aaca
//
LOCUS       DAJSBN010000214          509 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJSBN010000214 DAJSBN010000000
VERSION     DAJSBN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.227"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          228 a           70 c           82 g          129 t
ORIGIN      
        1 gaattcctta ggcagctttt gctgtgtaga aaggaataaa taataacgaa agttcaaaag
       61 ctaatgccga ctattctaga cattaaagaa gacgaaaacg aacgataaag aaagaaaagt
      121 tatgaataat cgtttattga aaaaaacttg aaaaaataca aaaaattcgt tgaccaagct
      181 aaatttagtt ggtatattat tacttgtcca aaagacagaa gaaaatattt attcaaaaac
      241 ataacaaaaa agttattgac atgttactaa cgacatgata agataactga gtcgttaaaa
      301 aagaaccact tgcgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
      361 aacaaacgat aagttatgat aaatgagttg ccgaaagatt tcgaaaaacg aaatcgaaaa
      421 aaacttttaa aaaagagttg acaatcgctt gtctctctga tatgatataa aagttgctac
      481 ggcagccaaa aatgaatcgg gaaacactc
//
LOCUS       DAJSBN010000215          499 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJSBN010000215 DAJSBN010000000
VERSION     DAJSBN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.228"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..138
                     /locus_tag="ONT86_003018"
     CDS             <1..138
                     /locus_tag="ONT86_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HCR2353713.1"
                     /translation="VDAATYYGNGLYCNKEKCWVNWGQSWSEGLKRWGDNLFGSFSGG
                     R"
     gene            138..488
                     /locus_tag="ONT86_003019"
     CDS             138..488
                     /locus_tag="ONT86_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2353714.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          201 a           54 c           85 g          159 t
ORIGIN      
        1 gttgacgcag ctacttacta tggaaatggt ctttattgta acaaagaaaa atgttgggta
       61 aattggggac aatcatggtc agaagggtta aaacgttggg gagataatct ttttggtagt
      121 tttagtggag gacgataatg acttcaataa acattgatca cacaaaaaaa gtcatacatg
      181 aattatacaa tagtttaatg acacgaccta atcagacgaa agcattatta gatattactg
      241 atgttttatt tcaggtatat aaaaaaattg atacagcaaa atatcctgaa attcttgtaa
      301 ataaattagt taattatatt tacatcgtgg gttttgataa taaaatacgc tttattgata
      361 atgatgaaaa attattaata gaattagggg atatttctaa aaaagctgga attaatagta
      421 aatacaaagc aaactttaca gataaatcgc aattttatag ttattcagaa acaatgcctc
      481 aacgataaaa tatagtata
//
LOCUS       DAJSBN010000216          487 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJSBN010000216 DAJSBN010000000
VERSION     DAJSBN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.229"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..>487)
                     /locus_tag="ONT86_003020"
     CDS             complement(<1..>487)
                     /locus_tag="ONT86_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR2353715.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DAJSBN010000217          462 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJSBN010000217 DAJSBN010000000
VERSION     DAJSBN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.231"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..430)
                     /locus_tag="ONT86_003021"
     CDS             complement(<1..430)
                     /locus_tag="ONT86_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2353716.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAJSBN010000218          452 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJSBN010000218 DAJSBN010000000
VERSION     DAJSBN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.232"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>452
                     /locus_tag="ONT86_003022"
     CDS             <1..>452
                     /locus_tag="ONT86_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353717.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DAJSBN010000219          436 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJSBN010000219 DAJSBN010000000
VERSION     DAJSBN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.233"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            227..424
                     /locus_tag="ONT86_003023"
     CDS             227..424
                     /locus_tag="ONT86_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2353718.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           61 c           78 g          139 t
ORIGIN      
        1 ttacgtaaat ggaaagtttt gtctcgctta atttgacaaa taataatatt taaaccgtat
       61 atcgagtgat tatccttgtg ttagcattac atccgtgtta tagtaaataa gtaatctaat
      121 ttgaaacgta atctgagcga tatattcaca ctataaaaac tccttttacc aagtaatatt
      181 aattgcaata aaacacgtat tatatacgta tcaggaggaa atatatatga ataacggtac
      241 agtaaaatgg tttaactcag ataaaggttt tggtttcatc actggtgaag atggaaatga
      301 cgtattcgct catttctcag caatccaagg agaaggcttt aagtctttag atgaaggcca
      361 agcagttact tatgatattg aagaaggaca acgtggtcct caagcagtaa atatcgtaaa
      421 ataatgttga acgtta
//
LOCUS       DAJSBN010000220          415 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJSBN010000220 DAJSBN010000000
VERSION     DAJSBN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.234"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..339)
                     /locus_tag="ONT86_003024"
     CDS             complement(<1..339)
                     /locus_tag="ONT86_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2353719.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAJSBN010000221          411 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJSBN010000221 DAJSBN010000000
VERSION     DAJSBN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.235"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            143..400
                     /locus_tag="ONT86_003025"
     CDS             143..400
                     /locus_tag="ONT86_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353720.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          183 a           50 c           56 g          122 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt attactataa ttgatttata c
//
LOCUS       DAJSBN010000222          409 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJSBN010000222 DAJSBN010000000
VERSION     DAJSBN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.236"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..379
                     /locus_tag="ONT86_003026"
     CDS             <1..379
                     /locus_tag="ONT86_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCR2353721.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DAJSBN010000223          404 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJSBN010000223 DAJSBN010000000
VERSION     DAJSBN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.237"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>404
                     /locus_tag="ONT86_003027"
     CDS             <1..>404
                     /locus_tag="ONT86_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353722.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DAJSBN010000224          343 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJSBN010000224 DAJSBN010000000
VERSION     DAJSBN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.denovo.239"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          126 a           57 c           54 g          106 t
ORIGIN      
        1 attagcgatg cagggatcga gagaaacatc aaaagggtat ccttcaagat cacgttaaac
       61 aagcctaccg ataaaaagct ctcagaagcg ttctgagagc ttttgacatt ttcttgtata
      121 attttatcct aacacattac aaagcaccta attcgagctt atatcaagca aatttgctat
      181 ttattactca atacatatct aaaaattact tgatggaaaa ttagatacat ggtaagcatg
      241 aaaagaaaat caatttgagc agatgcagta caatacatag caatttaaaa gctctcagaa
      301 ggcttctgag agcttttaat atattctttg ataatttgtc ttt
//
LOCUS       DAJSBN010000225        15704 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.guided.061, whole genome shotgun sequence.
ACCESSION   DAJSBN010000225 DAJSBN010000000
VERSION     DAJSBN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.guided.061"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..622
                     /locus_tag="ONT86_003028"
     CDS             <1..622
                     /locus_tag="ONT86_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR2353723.1"
                     /translation="AQDEPAIDEIYHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTD
                     LFFGATNNGRKERAESYKQEFIKRDVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRG
                     ILELLREGQQGVTLRTFESMYFQKKLVTDNQAIAHYHFYRPENIFLLRERDLDELPEF
                     LHTPFKPILSELLEFYDAENWAKRFITNDSTIYEQYEYDLSSVENK"
     gene            complement(772..1713)
                     /locus_tag="ONT86_003029"
     CDS             complement(772..1713)
                     /locus_tag="ONT86_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR2353724.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1839..2960
                     /locus_tag="ONT86_003030"
     CDS             1839..2960
                     /locus_tag="ONT86_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR2353725.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3218..4720
                     /gene="eat(A)"
                     /locus_tag="ONT86_003031"
     CDS             3218..4720
                     /gene="eat(A)"
                     /locus_tag="ONT86_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR2353726.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4748..5314
                     /locus_tag="ONT86_003032"
     CDS             4748..5314
                     /locus_tag="ONT86_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2353727.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5525..6586
                     /locus_tag="ONT86_003033"
     CDS             5525..6586
                     /locus_tag="ONT86_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR2353728.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6589..7485
                     /gene="murQ"
                     /locus_tag="ONT86_003034"
     CDS             6589..7485
                     /gene="murQ"
                     /locus_tag="ONT86_003034"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR2353729.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7499..9481
                     /locus_tag="ONT86_003035"
     CDS             7499..9481
                     /locus_tag="ONT86_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2353730.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9546..9992)
                     /gene="nrdI"
                     /locus_tag="ONT86_003036"
     CDS             complement(9546..9992)
                     /gene="nrdI"
                     /locus_tag="ONT86_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR2353731.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10203..11627
                     /locus_tag="ONT86_003037"
     CDS             10203..11627
                     /locus_tag="ONT86_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2353732.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(11808..12581)
                     /locus_tag="ONT86_003038"
     CDS             complement(11808..12581)
                     /locus_tag="ONT86_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR2353733.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12578..13483)
                     /locus_tag="ONT86_003039"
     CDS             complement(12578..13483)
                     /locus_tag="ONT86_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2353734.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13637..13957)
                     /locus_tag="ONT86_003040"
     CDS             complement(13637..13957)
                     /locus_tag="ONT86_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCR2353735.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14431..15117
                     /locus_tag="ONT86_003041"
     CDS             14431..15117
                     /locus_tag="ONT86_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCR2353736.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGIASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            15119..>15704
                     /locus_tag="ONT86_003042"
     CDS             15119..>15704
                     /locus_tag="ONT86_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 family protein"
                     /protein_id="HCR2353737.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVIS"
BASE COUNT         4974 a         2769 c         3356 g         4605 t
ORIGIN      
        1 ggcacaagat gaaccggcaa tcgatgagat ttatcatttt gattttttgg aagcaaaaga
       61 acttgggctg aaacataata gttcatttta ttcgaaacat atgaattttt cttctagcga
      121 accaaagacg gatctatttt ttggtgcaac gaataatggg agaaaagaac gagcagaaag
      181 ctataagcag gaatttataa aaagagatgt tacgaccaac tatttcattc ttccacatcg
      241 agggaatgaa caacctggat atcttactta tgaagagtac ctgaaaaaaa caggggaatc
      301 gcgcgggatt ctggaactgt tgcgggaggg tcagcaagga gtgactttgc ggacatttga
      361 atccatgtat tttcaaaaga aactagtgac ggataatcaa gcgattgctc attatcattt
      421 ttatcgtcct gaaaatattt ttttgcttcg agaacgtgat ttagatgaat taccggaatt
      481 tctgcataca ccatttaagc caatcctatc agagctattg gaattttatg atgcagaaaa
      541 ttgggcaaaa cgatttatca ctaacgattc cactatttat gagcaatacg aatatgacct
      601 ctcctctgtt gaaaacaaat aaaaaaccta cattttgaag agattatgta ctgaaacaaa
      661 caagcacaat tcttatggca tcatgagagt tgtgttttct ttttgatctt ttattgggct
      721 taaaaaaaga gaaccagctg ctgattcagc tgattctcat cagagagcct tttattctgc
      781 gccaaggatt ccttcttttg catggactaa ttgggtcaaa aaggcacaat atggtgtagc
      841 aatatcatat ttttgtcctt ttcgccaaat ggcaccattg atgtagtcga tttcagtcaa
      901 acgatggttt ttgatcaggt cttggtacat agatgggtaa tgcaacccga tgttgtcagg
      961 atcataagta gaagcaatat gttggtacac ttcttcttga tctagtatga ttccttcttt
     1021 tgctgcgata gaagcaaatt cagatacgat tgttcgaacc atgctctctg ccgctttttg
     1081 cgcgcctagt tcagcaatat tacaatccaa aatcgtgcat aaaccattca atgttccatt
     1141 tacacaagct tttcgccaaa tggaataacg aacgttatgg ctatagatcg gatttaaatt
     1201 tgcttcttga aagacttcta caactttttt tgtaaatgct tcgccttctg gttccaagtt
     1261 ctctagttcg atttctccat cacctaacaa tgtgactttt ccgggaccag ctagaccagc
     1321 tgtccacatc gtgatcccaa ataagatatt tttctttggc acatattttt ccagtacatc
     1381 ttcatgacct aatccattta gtaaacacaa aacataagta ttgtcagtaa taatggattg
     1441 gatcgaacaa aacatatcat ctaattgatt ggcttttgtt aatgctacga tcaagtccac
     1501 gtgttcgttg ctttcgatga tttcttctgg tgaatagatt ggcagttttg cgaccacttc
     1561 tttaccgtta aagtcagcaa tcaacccatt tgtacgaatg gcttcaatgt gagcaggcca
     1621 acgatcgatc aagaggacct catttccgct ttgatgtagc atcaatccga ttcggctgcc
     1681 cattgctccg gccccagcta ttgcgatttt catgtttttt ctctccttta ttcaatgagt
     1741 tcccttttag tatatagaaa aatggaagaa aatagcattg ttttgtttga aaaaaatgaa
     1801 aaaaagtgtc ttacttaaga agaaagtgag ggatacagat ggatattttg gcggcaattg
     1861 attcatttaa aggaagtgct tcttcatttg aactaaatca ggcagttcta aatggctggg
     1921 aagatcgtaa aggaagaaaa atcaatgtgc cgattgcaga tggcggagaa aatactgcag
     1981 aagcaatcta ttacgcgctc aggggaacat ggcgtaccat tgatgggatg gatctcttat
     2041 tccgtcagcg aaaaatcagc tatttattga caacttggca aggaaaaaac accgcagtca
     2101 tcgaatctgc acaagcattg gggttagatc tacttgataa accaacggat caaactatcc
     2161 gacttgcttc cagttatgcc ttaggagaac ttattaaaga tgcactgctt caagaagtag
     2221 aacaaattat tgtcacttta ggaggaagcg ggtgtagcga tggtggtcta ggattactcc
     2281 aatccttggg tgcttcttta gaagggtaca cagaaggaaa tcctttatta tctactaaaa
     2341 aacttcattt tgatcgaaga aacgttcttg tttctaaagt aaaattgatt atcgctgcag
     2401 atgttaccaa tccatataca ggtcaacaag gagctgcttg gcaatttgga agacaaaaag
     2461 gaggaataga tacgacgcta gctttcttag ataaacaagc aggaaagatt gctcagcaaa
     2521 taaaagattt ctgggaggtg gatttagatg atattccagg aagtggagca gcaggcggta
     2581 ttggtggagc cctttttttg ttgggagcga agatggtttc tggtttcgat ttagtcagcc
     2641 aaatcactgg attggaaaaa aagatcgaac aagcagatgt catcattaca ggagaagggc
     2701 ggatcgatca gcaaacgatc cacggaaaag ttccatacgg tgtagcagtg cttgcaaaaa
     2761 aatatcgaaa accaataatc gctatttgcg gaagtagaga ccaagagcta gaaggacttt
     2821 ctgattttct cccaattgtc ttaagtattc atttgggtcc aataggttta gaagaagcaa
     2881 tggaacacag gcaaacatta gaaaaaacaa aaatcttagg acagacactt agcagatttt
     2941 tcaccatcct cccgaagtag ttgacaaaat cattttcttt atgctatcgt aatttcaaat
     3001 ataggacaaa gtgagggatc cgtgcattga aaaattaagt cgattgatga aagctgcatt
     3061 cagactgtac cttgtggaga caaggaagca gtgtgcagat tttcgggatt ggcttggttt
     3121 tttcgatgta tgggtctttt tgtgtgtatc caacccttgc atcctcttta gccagttctg
     3181 aaaataggac tggtcttttt ttacgaggtg atggaaaatg tctaaaatcg aaataaaaaa
     3241 tctgacattc ggctacgaca gccaaggcac attattattt gaacaagcaa atctaaattt
     3301 tgacacacaa tggaaactag gacttatcgg acgaaacggt cgaggaaaga caactttact
     3361 gaatattcta caaaacaaac taccttatca agggcaagta atccatcagc aagaatttgc
     3421 ctatttcccg caacagacaa aagataaaga acgtttaacc tattacgtgt taaatgatat
     3481 tacggatttt gagatatggg aaatcgaaag agagctccaa ttgatgcaaa cagatcctga
     3541 aatcttatgg agagaattca gcacactatc ggggggagag aagacaaaag tcctactggc
     3601 acttttattt gtggatgaca ctcatttccc gttaatcgat gaaccaacga atcatttgga
     3661 tatctctggt agaaaacaag tagcggctta tttgaaaaag aaaaaacaag gcttcatcgt
     3721 ggtcagccat gaccggggat ttatcgatga agtagtggac catgttttag caatcgaaaa
     3781 aagtcaactg gaactttatc aagggaattt ctctatctat gaagaacaga aaaaacttcg
     3841 tgatgaattt gaaatggctc aaaatgaaaa attgaaaaaa gaagtcagta ggctaaagaa
     3901 aacagcagct gaaaaagccg aatggtctcg ttcccgagaa ggagataaaa caaagaaaca
     3961 agtcggattc atcgatactg aatctagacg agtgaataaa ggagcagtgg gtgctgatgc
     4021 tgcacggacg atgaaacgat ccaaagcaat cgtgaatcgg atggagaccc agatcagcga
     4081 gaaagaaaaa ctattaaaag atatcgaata tatcgattct ttgacgatga atagccaagc
     4141 gtctcaccat aagcgacttt taagcgtaga agatcttcaa ttagggtatg aaaatctgtt
     4201 attcgagcca attcatttta caatcgagcc tcatcagcgg gtggcgattt caggtcctaa
     4261 cggtgcagga aagtcatcca ttatccatta tcttctgggg gcattcaacg gcaaggttat
     4321 aggagaaaaa taccagccaa aacatctgag cattagttat gcaagccaaa attatgaaga
     4381 caatcgagga acgttggcgg aatttgcaga gaaaaaccaa gtagactacc aagcattttt
     4441 gaacaacctc cgaaagcttg ggatggaaag agatgttttt cataacaaga tcgagcagat
     4501 gagtatgggc caacggaaaa aagtggaatt ggctaaatct ttatcacagc cagctgaact
     4561 atatacatgg gatgaaccat tgaattattt ggatgtcttc aatcaagaac aattagaaca
     4621 actgatcttg aacgtgaaac ctgccatgtt actagtggaa catgatcaaa ccttcctgga
     4681 taaagtatct actgagatta tttctcttga gagaatctaa cccttgtaat ctcaagaaca
     4741 acaaggtatg gttaagggga actttgccaa cgaaggagag aatcacatgc tttatgtaac
     4801 gagacacgga gaaacaactt ggaacgcaca aggattagtt tgcggacggg cagatgtgcc
     4861 gctgactgaa aaaggacaga tgcaggcaca gaaactagca gaaaaggtag tagatttgcc
     4921 tgttccaatc acgaaaatca tccattcccc tcttcaacga gcaagagata cagcacaagc
     4981 agttgctgat cgtctcagct tgccgctgac cgttgatgaa cgattggtag aaatggattt
     5041 tggggattat gatggactgc caagcaaaga tgagaacttt caaaaagcac gtttagcgtt
     5101 cgctgtacga ttcccaaatg gggagtcagt cttagatgtg tatgcacgga tcgttccttt
     5161 attgaaagaa tgcatcgaag acgaagaaaa tgtctatctg ttagtttgcc ataatgcctt
     5221 gataagagtg atcaatgctt attttcatcc gatgcctaat gaggggttct ttacattcat
     5281 ggtagataat acagaattgg tttcttatga gtaattatgt ttttgtgtta tttaaataaa
     5341 atatttcaag gcactgcttt tgcttagcag tgcctttttt aggttatgac aattcttttg
     5401 ataatataaa ctcaaccttt agaaatgaaa tttaattatt ttaataaaag tattgaaatt
     5461 ttgtttcttt agatatatac tctagtcata gaaatgaaga cggatacaga aaaagaggga
     5521 taatatgttt ggcttttcaa tatttatgaa cgattctttg tcagaagaga agaaacagta
     5581 tatccgccaa atggcatcca atggctttgt cggtattttc acttccatgc atataccaga
     5641 agatgatgtt tcggcatatc aaaagcgatt gatcgaacta ggaagcgaag cgaaaaagaa
     5701 taaactagaa ttgatggtcg atatttcagg agaggcattg gaacgagcgg gtttttcatt
     5761 agaagatcta acgcctttga aagcaattgg tgtaacaggt ttaagaatgg attatcatat
     5821 ttccaatcag cagattgccg actggtcaca tcaattgaaa atcagtttga atgctagcac
     5881 gatcacgcct aaagatatcg atgaattgaa agaagcagaa gctgatttct cacagattga
     5941 agcttggcat aattattatc cacgtcccga aacagggctg gacaaagaat ggtatcagaa
     6001 aaaaaatcaa tggctcaaaa ttcaaggtct ccttgtccaa ggttttgtac caggagatac
     6061 agagttgaga ggcccactat atcaaggatt gccaactttg gaagaacatc gagggatcca
     6121 tcctcttgca ggcgctctag atctattagc aagtaacacg gatattgttt acatcggaga
     6181 tgcaggtctg tcagaaaacg ttcaggagca atttgcttct ttccaaaaag aacagacagt
     6241 cttgctccat acggaacctg tagatgaaga attttacgaa tatatattag ggaagcatac
     6301 gaatcggcaa gacgatgcaa gagatgtcat acgaagcgta gatgcacgtt ttcgagagat
     6361 tccgcctatc ccagcaagaa atacagctac gcgtatgaaa gggtctatta ctttagataa
     6421 cgaaaagtat ttgcgctata tgggtgagat ccaattaacc aaatatgatt tgccagcgga
     6481 tgaaaaagtg aatgtagtag ctaaagttat caaagaagag ctaccattga tcaaccaaat
     6541 caaagctggt atgaactatc aatttataag aaaagaagga agataaaaat gagcaaaatc
     6601 aatttagaga acctaacaac ggaacgcaga aaccaagaaa cttttggatt agatgagatg
     6661 agtatttctc aagccgttca actaatgaac aaagaagatc agaaagtggc cgctgcggtt
     6721 gaaaaagaac tgacaagtat cgaaccagtg attgctaaca cgattgagtc ctttaaaaaa
     6781 ggtggacgtc tgatttatat gggagccgga acaagtggaa gactaggtgt gttagatgct
     6841 gctgaatgcg taccgacatt cggtgtagaa ccggaaatgg tcataggtct aattgctggt
     6901 ggagaagaag caatgactgt agctgtcgaa ggggccgaag attcttatga gctagggcaa
     6961 gaagatctaa tcaagctgaa tttgacaaaa aatgatatag ttattggtat cgcagctagc
     7021 ggtcgtacac catatgtgat cggtggatta gattacgcaa aatcgatcgg tgctgtcaca
     7081 ggaacgattt cttgcaataa acaagccgaa atcagtaaac acgcggattt cccaatcgaa
     7141 gtcgatgcag gtccagaatt tttgacaggc tctactcgat tgaagtcagg aacagcacaa
     7201 aaacttatct tgaatatgat ttctactatc agtatgattg gtataggtaa agtattcaat
     7261 aacttgatgg tcgatgtcaa accaacaaat gaaaaactaa tcgaacgctc aaaacgaatc
     7321 atcatgcaag cgacagaagc tgattacgaa acagctgaaa agtactttga agaagcagat
     7381 caaaatgtta agctggctat cgtcatgatt ttgacaaata gtgataaaga ggaagctcaa
     7441 gcaaaattag ctgaagcaaa cggatttgta aaagggacct tatgaggggg aaaagaccat
     7501 ggcaaaaaac gaactgactt tggaagaact ggcaaaacaa atttataaag gggcaggcgg
     7561 catgggcaat gtagaaagca ttgtccactg catgactcgt gtgcgtatga agatcatcaa
     7621 tgatgatctt gtaaatgtct ctgaattaaa agcgattcca ggagtgcttg gtgtagtaga
     7681 agatgaacag cttcaagtca tcatcggacc tgggaaagtg aacaaagtag cacagcaaat
     7741 ggttgatttg gcaggcgtga aattaggaga aaaactttct tctgtaagta cagctacagc
     7801 aaattcatca gcatctggaa aagaccgtgt aaacgaacgg gcacaagaaa tgaaagcggc
     7861 acaaaaatcg aaacaaaaac catctaaaat caaatcggta ttaaaagaca tttctaatat
     7921 ctttgtgcca atgattcctg catttgtcgg tactgggatc gtggcaggga ttgccgcagt
     7981 tttagcaaac ttagtcacag ccggaacact tgatgcagca acatggcaac aatatatcga
     8041 tgtgatgaac atcttgaaaa acgggatgtt tagctaccta gttatctata caggtatcaa
     8101 tgctgcacaa gtttttggtg caacaccaac tcttggtgga gttatcgggg ccgttgtgat
     8161 gctgacaggg atgaatccgg aagcaccact gaaaaactta tttactggtg aagctcttgc
     8221 agctggacaa ggtgggatcc taggtgtcat cttcgctgtt tggctattgt caatcgttga
     8281 gaaaaaactg cataaagtag tacctgatgc ggtagatatc atcgttacac caactctttc
     8341 attgattgcc atcggtctga ttgaaatttt cttcatcatg cctttagcag gtttcatttc
     8401 tgatggcatg gtcggtggga tcaactgggt acttggcgtt ggtggagcat ttgccggctt
     8461 cgttctaggc acattattct tgccgatggt tatgttcgga ctgcatcaaa ttttgactcc
     8521 aatccatgtg caaatgattg atgaaactgg tcgtacgcta ttgcttccaa ttcttgcgat
     8581 ggctggtgca ggacaagtgg gtgcagcatt ggcattatgg attcgctgca aaaaagataa
     8641 agaattaaca gaaatgatca aaggagcttt accagtcggg atcctaggaa tcggcgaacc
     8701 attgatttac ggagtaacat tacctctagg acgcccattt attactgctt gtatcggtgg
     8761 aggtatcggc ggggcagtga tcggaatgat cggaaatgtc ggggctatcg ctattggacc
     8821 ttcaggagcc gctttgatcc cattgatctc tgacggtaaa tggttaggct acatccttgg
     8881 attattggca gcttatgctg gtggcttcgt tgctacattc ttcttcggta ttcctaaaga
     8941 ccgtttagaa aaagaagaat tagcgactga aacagccaca gtaaatgaag tgcctgaaaa
     9001 agtagtacct caaacttcag ctgaaagtgt gactatcctc tcatcagttg ctgacggaac
     9061 agttgtttct ttagataaag caagtgatcc agtttttgca caaaagatga tgggtgaagg
     9121 ctactttgta gaaccagaaa acggacatat ctattctcca gttactgggg tagtcagctc
     9181 tgtcttccca acgaaacacg ctattgggat cacaacgcca aatggtttag aaatcttgct
     9241 tcatatggga atcaacacag ttgacttagg cggtacgcca tttgatgtga aagtaacaga
     9301 agggcagcaa gtgacacctg atacgcttgt agcagaagct gacttagaag cgatcaaagc
     9361 agcaggcaaa gaaacatcca tgatcgtctt ggtaaccaat atggatcgtg taaaaaactt
     9421 tgtcttggaa aaaacaggta aagcaaaagc aaaagcacct gtgatggatg tcaaagctta
     9481 atatgataga aacaatagaa acgaaagaag aaactgaaat ctatttttca gcttcttctt
     9541 ttttattatt tattgttttc tgctaaaatc tgataaattc gtgtcacatc agatggtgtt
     9601 ccccgtaatt cataatctgc caagaaagga aagccaaatg tttgtgcata ttgtttggct
     9661 gttagacaat actgattatt gaagttttta tttccgctac cgactacacc aagacaaagt
     9721 ttatgattgt cttcatgttc cagatattca cgcatcgttt cggtcaagat ttctgtatca
     9781 ccgttatcta aaccatttcc gccgtctaaa taagtaggaa cgaaagtaaa aaatggttct
     9841 gtttctttcg taaaatcact attttcatgg atttctttta aagtaacttc aggattgaca
     9901 ggatcttctg catgcatttt ttcagcatat tctgctagat gtttggcaaa ggcacgcgta
     9961 tttcctgaaa tcgatatata taaaatgttc atggatcatc ctccaattct ttccttttag
    10021 tatagcatct tttttaagtt ttttggtggt gtaatttgga aagggactag gcgctttgct
    10081 tgtataatga gaaaaaagcc ggtagaatta tatttctatt aaagttttta ttaattaagt
    10141 aaaaataaaa aaggtttgtt atactttttg ataaaagcgg ttttattttg gaggatgctt
    10201 acatggatac agagcaacag aaaaaaggaa ttggtttaat tccacttgct gccttagtaa
    10261 ttggttcagc gattggaggt ggcgtatttg gtattatgac ggacatctcc ggatcggctg
    10321 gcggcccagc tattttcagt tgggtactcg taggaacagc aatgttgatg ctctccttgt
    10381 cgatcaataa tatcaataaa aaacgtccag atttaaaagg cgggatcttt agttacgcag
    10441 aagaaggttt tggccgtttc gctggtttta tcagcggttg gggttactgg ctcacatgct
    10501 ggttaggcaa cgtagctttt gcaacattgt tgatgagcgc attaggatat ttcttcccag
    10561 tatttgaagg tgggcaaaat ctggcatcga tcgtgatgag tacagtcttt ttatggggat
    10621 atgcatggct agtgaatcgg ggagtcgaaa atgctagttt cgtcaatgct atcgtcacat
    10681 tctgtaaatt agtgccatta tttttattta ttgtagtagc tatcagcacg ttttcgatcc
    10741 aacaattcat tgataacttt tctagcgaca ttatattgaa tgcgaatgga cagccgatcc
    10801 cgttttccag tcaagtattg agttgcatca tggtgatgct ttgggtattt gttggtattg
    10861 aaggagcctc tgtcgtaggt tcacgagcaa aaaaacaatc agatgttggg aaagcaacga
    10921 ttctagggat attcggcttg attattattt acgtcttggt ttccatgctt ccatatggga
    10981 caatgacgat gaatcagctg caaacgatca aggcgcagcc tgctatgggc tatgtgtttg
    11041 aaatgatggt tggtaaatgg ggagcagtcg tgatcaacgg cggtttgatc atttctttag
    11101 ttggtgtttg gctctcatgg acgatcttac cgattgaaac aatgggaaac atggctgaag
    11161 acggactttt gcctaaagca tggggaaaac tcaacaaaaa tggggcacca acttatgcta
    11221 tcgtattgac gacgttatgt acgaatgtct ttttactttc attattattc actgacagtg
    11281 cttataactt tgcttacaca cttggtacag cagctatttt cttcacgtgg cttttcttag
    11341 gattgtacca aatgaaactt tcttatcagc ggaaagaatg ggtccaattt ttagtagggc
    11401 ttttagcaag tgcctttcaa gtatgggcta tgctagtcgt agcctttaat gaagtaatgc
    11461 ttgttcttgt tcttttcttg ccaggcattt atttttacct caaagcacgt aaggagcaaa
    11521 acgagcctgt acaacatatc cacttagata agattttact tataggtgtg acgatggtcg
    11581 gagtgattgc aatggtttta tttgccactg ggatgcttca cgtatagaag tataaaagaa
    11641 gtataaagag aggttgtgag cctgtcgttt attcaaaaac atactaggaa aggagctgtg
    11701 acacaaatgt tgtcacagct cctttttcag aataaacggt gtttaagaga cagccaatcg
    11761 gcaataaggc tgaacgtctc tttcacagcc tcttttttga tgagtcatca tacatttttt
    11821 cgcttaaata cgagatatcc taatgctaag aacaaaacga tgtaagctaa attaccgaat
    11881 agcatttgat tagtagaaag atgagtcaat tgcttcatga tctcttcgtt tccaatttgg
    11941 ttttgcagat tcaacatact gatcggattc catttgatcc actcccattt ttggatgagt
    12001 gcaataagga taccagatat gattgacgaa gcaaaataaa agacgattcc tgctgagatg
    12061 gatgcgccgg aactattgat aaagcaagca atcatcaaca ctaaacttaa aatcagccat
    12121 aaaccgatat aactacccaa agtataagtg aacaagcttt ggatcagtgt ttgaccagta
    12181 gaaaccttct ccgtgaagga aatcgaaggg aaaagaatca gtttcatcaa cacagtgaca
    12241 ataatagcta aaaggtataa ataaacagaa taaataacta atgtgatcca tttgcttact
    12301 aatatttctc ctcgagaata ctttcgataa aggagatttt tcaatgtacc atattgtgct
    12361 tccatcgaaa tgatcgtgct tgcagctgca atcatgataa agacaatcca agatgtggca
    12421 ctaaaaagct gagcagtcat ttcaactgga tcaatgatcg tcgtgtattt tttggctaga
    12481 agtgctgttc ctatgagcaa aacaactaat agaacagata aaacagcagt tgactttttc
    12541 ttgatcaatt taaataattc ttgttttaca agcaatgtca tttttcttct tcccccttta
    12601 ctgaatccag aatttccaat agagaagatt ccaaatcatt cgtcagatgc tggacgtctg
    12661 tcacatggat attttgtgca gacaatagct ggatcacggt tcctaataat tcttcgtttt
    12721 cttttaaaag cttgatttta tcaggatctt ccaaagtata tccagcttct gtcaagatcg
    12781 ttcgagcagc tagatcattt gttgtagaga taacgatgaa atcttgcgct gtttggttca
    12841 attcagccat tgtacattgt ttgatgatct ttccttgatt gatcaggatc agctcatcga
    12901 ccattttttc aagctcgctg agtaagtgac tggagatcaa gaaagaggtg ccctgagtag
    12961 ccaagtcggt aatgattttt cgcacatctc ttgttgcttg tggatccaaa ccgttcatcg
    13021 gctcatctaa aataacgata gacggatggt tcagtaaggc aagagcaatt cccaattttt
    13081 gtttcattcc caacgagtag gatttcgctt ttttgtgaat gtacttttcc atcttgagct
    13141 gattcacgat agtgtcgata gcagatacat cgttggtttc agagaataac ttcagatgat
    13201 catagcctgt taagaatgga tagatacccg ggtattcgat caatgctcct acttgttcca
    13261 accctttatg actagaggga gaaatctcat tttcaccaat acagattgtt ccatctgagt
    13321 aatggatcag tcctaaaata gctttcatga tcgttgtttt tccagcgcca ttaggaccta
    13381 ctaatccaac gattttgcca ggggataagc taaaactgac atcgctgagg gcttgtttgt
    13441 gaccaaagtt cttggataat tcttggaccg aaagactttt caaaaatatt cactcctttt
    13501 ctatagatag tacaagcata cacctgatga gtggagaagt cgctaggtca ttggacttat
    13561 ttcttatttt ttcataaaaa aattgaatat taaataaaaa ggcttatttt ttctgatcag
    13621 tacttattcc aagtgtctag tggccgctag taatcttgat gccaatgata ctgatgagta
    13681 gcatccccac aaagaaccaa gtggaaagat tcagcttatc atgaaacaag accacaccaa
    13741 ttatgaccga accaactgcg ccaatccctg tccagatagg ataagagaga ctcatcggca
    13801 aacttttgat tgcctttgac aaaaagtaaa aactagcaat catgccaatc actgtgtaaa
    13861 aagaataatt gattttagaa aaaccatcac tccatttcat catcgttgac cagaatactt
    13921 ccaaaattcc tgctatgatt aattccatcc atgccatact tacattcctc cttaactata
    13981 aaaaaataca caaaaagagg aaaatcatcc cttcaaagac ctaatggaat gatcatcctc
    14041 tttatcctac ccggtggcag attgtatatc tttttctgta gtatagcaca cctatccaga
    14101 tatcggcaat ctcatacttt ttagtaaagt gtaccaagca ctgacggata tatcgttagc
    14161 ggaaaagaag atgaatgctt tgcaatttca tagaaagaag ttgttattta ttaatcattt
    14221 aaaaaaaaca gtaactagac cttatttcta tatattatcg ggcaataaat taaaaaaagc
    14281 tttaccttac aataagtaag ataatttttg tgataaaaaa ataacgaaaa atatttaaaa
    14341 atagtgtcta tttcgtgaga aaacaactat attttttatg tgttttgctt tataattaaa
    14401 aaaatattgt cattttatgg aggaaaatat atggaaaatt atcttagcct tttaggcgtc
    14461 ttgattgtta ttctcgggtt tgcatttaaa ttagactcga tattgatcgt aatggttgct
    14521 ttagttgtta cagctttgac tggcggtctt ggcattcaag gaatgttaga acttttaggt
    14581 acaagttttg taaacaatcg cgggatggcg atttttatca tcattatgtt ggcaacagga
    14641 acgcttgaaa gaaatggtct gaaagaatca gcggcttcct tgatcaagcg attcaagaaa
    14701 gtatctgctg gtatgattgt agatatttat gggatctttc gaatgatctt tgcggctttt
    14761 aacgtgagtt ttggtggagt tgctggattt gttcgtccaa tcattttacc gatggcattg
    14821 gggaccatcg aatcaaaagg gttagaagtc catcctaagc atgaagaaga actaaaaggt
    14881 atagcttcag ctatggaaaa tgtctgctgg ttctttggcc aagtactatt catcggagga
    14941 gcaggggcgt tgctagtcca atcaacactg aaagatctag gatatgacgt aacattggga
    15001 cagcttgctt tagtggaagt tccagtggct atcacggctt tagttgtggc aagtgtctac
    15061 ttctatctta aagaaaagaa attagcgaag aaatattatc caggaaaggg gcaataagat
    15121 ggactttttt acaaatcctg atattttatt aggcgataaa ttactggaga tcctttatat
    15181 cgtgatggga ctcgtattga tttacactgg agtaagaaat ttaacagata aaaccaatcc
    15241 tcatcgttat gggtcagcct ttttctggac ggtcttaggg gtagttattg caggaggaag
    15301 atggcttccg gcaatcgtga acggtggctt gatctttgcg atgaccatac cagcaattgc
    15361 taagaaagtc agcaaaggag aaagccggct tccatcaaaa gaatatatgg aaaaaatgtc
    15421 agacaaacta ggaatgaaaa tttttattcc agctttgagt atcggtgtct ttgcgatctt
    15481 gtttgctcta tttaccaatt taggtgcttt agtcggcgtg ggcgtcggtg tttttgcagc
    15541 catgctaatc ttgatgttct tttctcgaga taatcgacca agtacatttt tagatgatgc
    15601 agcagatatg ctgggcacag tagggccgtt aagcatgttg ccaatgcttt tagcttctct
    15661 aggtgcggta tttacaagcg ctggagttgg gacagtgatt tcca
//
LOCUS       DAJSBN010000226         5873 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.guided.101, whole genome shotgun sequence.
ACCESSION   DAJSBN010000226 DAJSBN010000000
VERSION     DAJSBN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.guided.101"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(41..1027)
                     /locus_tag="ONT86_003043"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="ONT86_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR"
                     /locus_tag="ONT86_003044"
     CDS             1179..1874
                     /gene="vanR"
                     /locus_tag="ONT86_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR2353738.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="ONT86_003045"
     CDS             1852..3006
                     /locus_tag="ONT86_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR2353739.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="ONT86_003046"
     CDS             3221..4189
                     /locus_tag="ONT86_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR2353740.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="ONT86_003047"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="ONT86_003047"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR2353741.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5827
                     /locus_tag="ONT86_003048"
     CDS             5219..5827
                     /locus_tag="ONT86_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCR2353742.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1772 a         1144 c         1320 g         1637 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcagtgc
     5581 cattgatctt acgctttatc gattagacac gggtgagctt gtaccaatgg ggagccgatt
     5641 tgattttatg gatgaacgct ctcatcatgc ggcaaatgga atatcatgca atgaagcgca
     5701 aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg tttgaagcat atagcctcga
     5761 atggtggcac tatgtattaa gagacgaacc ataccccaat agctattttg atttccccgt
     5821 taaataaact tttaacggtt ctgttgcaaa gttttaaatc tactatcaaa taa
//
LOCUS       DAJSBN010000227         5586 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.guided.105, whole genome shotgun sequence.
ACCESSION   DAJSBN010000227 DAJSBN010000000
VERSION     DAJSBN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.guided.105"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            105..569
                     /locus_tag="ONT86_003049"
     CDS             105..569
                     /locus_tag="ONT86_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2353743.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            553..1182
                     /locus_tag="ONT86_003050"
     CDS             553..1182
                     /locus_tag="ONT86_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR2353744.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1536..3014
                     /gene="msr(C)"
                     /locus_tag="ONT86_003051"
     CDS             1536..3014
                     /gene="msr(C)"
                     /locus_tag="ONT86_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR2353745.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3061..3507
                     /locus_tag="ONT86_003052"
     CDS             3061..3507
                     /locus_tag="ONT86_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR2353746.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3706..4221)
                     /locus_tag="ONT86_003053"
     CDS             complement(3706..4221)
                     /locus_tag="ONT86_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2353747.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4338..5540
                     /locus_tag="ONT86_003054"
     CDS             4338..5540
                     /locus_tag="ONT86_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2353748.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1739 a          932 c         1196 g         1719 t
ORIGIN      
        1 agcgtttttt ttattacaca gctgattctt tgactttttt ctttttttat tctattattg
       61 acaagtcaat cgttgacgag tcaattatta gaaaggagga aattatgtct ttagaagaga
      121 ttagtcacct tttatatcaa atcaaattag ccgatcaaaa aatcgtccag atctttgaag
      181 aaaatatcgg tataagtctt actagatacg agatcatgat gagactgaaa gaagaaaatc
      241 cattgctaca aagcagtttg caagaaaagt tgcagattga tcaaggagca gttactcgtc
      301 atctgaaggt attggaagca aatggctatg tttcgcgtca gagaaatcct cagaacaacc
      361 gggaagtgta tgtagcgcta acagaaaaag cccagaagaa aatcaagcat tgtgaaatgg
      421 accataaaga tacaggttcc gtgctatccc cggctttcag cgatgtagaa atgaaacaat
      481 ttctaacact tttagataaa ttgaataccc aactttgcaa tataacaaca gaaacgaaag
      541 aggttaataa taatggaaca aaaaaatgat ttttcaacaa ttatgactgg tagaagatcc
      601 gttcgcgtat atgatgaaac atacaaaatc ccacacgaag aaatgctgga aatgatcaaa
      661 gaagcgacga ctgcaccttc ttctgtcaat atgaaaccat ggcgctttgt ggtcgctgaa
      721 agcgaggaag caaaagaaat cttgcgtcct ttgatccgtt tcaatacgag acaaaatgat
      781 acttcttctg caatggtcat gatttttggc gatatgcaat gttacgaata tggcgaagaa
      841 atctacaacc aagcttacga atcagggaag atgccaaaag aagtcagaga tcaacaatta
      901 gctgcgatca ttccttatta caagagtttt tcaagagaag aaatgaacga tgtagtcaaa
      961 gtcgattcta gcttagcggc tatgcaattc atgttagttg cgcgtgatca tggctatgaa
     1021 acaaatccaa ttggtggatt cgaagcagat cagttggcag aggcatttgg tttggataaa
     1081 gaccgttatg ttccagttat tattttatca ataggaaaag cggtagaaga aggttacgag
     1141 tctgttcgtt tagcaccaga aaagatcact atgttcgaat aaaaggtttt cagttactat
     1201 tcagtagcta ttgctttttt tgaatcgtgt gtggtaacct aataaaaaag gaggtcggaa
     1261 aaatgactgc atcgatgaaa ttacgtttcg aacttttgaa taacaactaa ataaattcaa
     1321 aagacatgta taaacaacaa tttcatggat ggagtatgtc cattttttcg aacgacagta
     1381 gaataggtga aagctatgac agatttggtg atttagtcat agttatttcg ttcagagtag
     1441 gaaaagatgt gatgcagttt ttttgcaaac agatttttct gctctttttt agtatcgata
     1501 attgttgttt tacatgaaat aggagtgtaa aacatatgga aaatttagca gtaaatataa
     1561 caaatctaca agtcagcttt ggcaaccagc tagaactatc tattgattct cttcgtgtct
     1621 atcagcaaga tcgtataggg atcataggag aaaatggtgt cggtaaatcg actttgctca
     1681 aactaatagc cggtgaactt tttcccgatc atgggaaaat ccaaacagag atcaccttca
     1741 actacctgcc tcaattaacc tatctcgctg aagcaaagga cctaaatttg gaattagcca
     1801 gtcatttcca gttaagactg gaagaaactt cagagcggaa atggagcgga ggggaagaac
     1861 gaaagatcga gttgatacgt cttctttctt cttatgaaca agggatgctt ctagacgagc
     1921 caacaaccca tctagataga aaaagtattg atcgactgat tgaagagctt cgttattatt
     1981 atggtacgct ggtttttgtt agtcatgatc gctattttct agatgagcta gcatcgaaaa
     2041 tctgggaagt aaaagacgga gaaatccgag agttttcggg gaattatagt gcctatctca
     2101 ctcaaaagga attggagaaa aagactcagc tacgagaagc agagtcgatc atgaaagaga
     2161 aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag
     2221 tgtccagtaa aaagaaaaag caacaaatca gactggatcg gttgtcttcc tctaaacaaa
     2281 aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac attagagaga agactccaaa
     2341 aaataggaga aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat
     2401 ctcttgaact ccacagccgc tatcccatca tgggacaaaa tgtccaattg gaaagaagtg
     2461 ggagaacatt actggtaaat ggtgattttc agttttcttt aggtaaaaaa atcgcgattg
     2521 tcggtgaaaa tggttcaggt aagacaacct tattagaaca tatccgcaaa caaggagaag
     2581 gaatccttct ctctccgaaa gtaagctttc aagtatatca gcaaaagggt tatcaaatga
     2641 catctgaaga atccatcatt cgttttgtca tgagacaaac agagttttca gaatcgcttg
     2701 tccgtagttt gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta
     2761 cattaagtgg gggagaagcg acccgtctga cgattgcttt gctttttact aagccaagta
     2821 atgtgttgct gttagatgaa ccgactaatt ttattgatat ggcaacgatc gaagctttag
     2881 agaagctaat gcaaatatat ccgggaacga ttttgtttac ttctcatgat tcttactttg
     2941 tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa aaagtactta
     3001 cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga gtggtacaga
     3061 ttgcctacaa ataaaaaaga aggaataatt tttacaacaa tgatgtgttt tttgatggtc
     3121 ttggggatga gcgcgtataa tttatggctg catcaagatc ttcatttatc aagtttgttg
     3181 attggtctag tgcctggatt cgtggtggct ttcattttgg atgtcttcat catcggtgtg
     3241 ctggcgaaaa aaattgcctt taaactacca gtaaatccag aaagcaaggt ccaaatgatc
     3301 ttaactattt catgcttgat ggttttagga atggtgacat gtatgtcttt attcggtgtg
     3361 atcatggaac aaggaatacc tgatcaatta ttctctgctt atatgaatgc ttggaaaatg
     3421 aatgtgattg ccgctttacc gttgcagttg atcatcgttg gcccgctttc aagaagtgtg
     3481 ctaaaagcaa ttcaaagtaa tgaatgatgg aacactgtta tttgaaaaaa ggccttggca
     3541 tgctttttgt catagtctct tgaatgcagt tctagaaaaa tagtgatccg aacgataggt
     3601 tttggcctgt cgttcggatc gctttttctt ttccaactgt attgctcttt tattgaagat
     3661 aatttgctct tcaatagata tttctagaaa ggttctgttt atcccttact caaaaaactg
     3721 ctgcatttgt tctcttactt gctgacacca ttggagttcc tgctgacaaa gcataattct
     3781 gcgctggatc acaacataac gtccaaattc ttgtttcttg tctagcgaga ggtcattcaa
     3841 ttttttttga tttttttgca tgattttgtt cagctgttgt tctctagtga atagcagtcc
     3901 agtagctgtt gttttatcga gcgtcgaaaa agcatagatt tttgctagaa attcatcttt
     3961 ttgcgtagga gcgggagttt cctctttgat ccattcctct acaagagcac gtccttcatc
     4021 agtcaagtgg tagatctttt tttgagagtg ctcgtctttg tccaaaacgg tcacataacc
     4081 ctgctggtct aacttggcaa gcgttgtata gatctgactg tgatgggctt cccaaaaaat
     4141 tgtgatcagc tgcttcaatt catacccaga acgtggtttg gataaaagca cgcataataa
     4201 tatataggct aaagtattca taaaaatcct acctctcgtc ttattttctt tattatatca
     4261 cagtaaagtt tccacgtttt acatgtgcat attgacatgt aaaataaatc atgtatgatg
     4321 ctaatagaga gaaagggatg gaaatattgg agaatcaaat agatatacaa gaacaaacaa
     4381 atgaagaaac aaaggggaaa agttcagcgc tagttgtagc agctacctgt atgatcgctt
     4441 ttatgggagt gggtctagta gacccaatct taaaaacgat tgctgcacag ttaaacgcta
     4501 cgccagctga aacgaccctg ttatttacta gttacatgtt agtcactggt gtggtgatgc
     4561 tatttacagg tttcttatct agtcggatcg gtttgaaaaa aacattgatg acaggattgt
     4621 ttatcatcgt tgtattcgct ggtcttggcg gattatctgg gaatatcgga atgctgattg
     4681 ggttacgtgc cggctggggt gtcgggaatg ctttatttgt ctctactgct ttagcaacga
     4741 tcgtcagtat tttagttggt aacacagaaa aagcaatcat gatgtatgaa gcagcacttg
     4801 gctgcggaat ggctgttggc cctctagtcg gtggggtctt gggcagtggt tcatggcgtt
     4861 atccattttt tggggtagca tttttgatgt tttgcggttt tatagcccta gcggtacttt
     4921 tacctgaaac tgagaaacca aagagaaaag taggcctttg ggaaggtgtc aaagcgttaa
     4981 gcaatcataa acttcgttca gttggactaa tcgcattgtt atataatttc ggattcttta
     5041 ctttacttgc gtatgcgcca tttttattga ttggctattc tgaattagaa gtcggttttg
     5101 tattttttgg ctggggtgtg cttctggcta ttgcttcgat tttcgttgca ccggctcttg
     5161 agcggaagtt tacgacttat cggacaattt tagcagcatt tgtcttattc attatttgtt
     5221 tgctgttatt aggagctgga acttctatgc cagtccttgt accgatcagc attgtgttag
     5281 caggattttt ccaaggaatt atcaatacac tgttgacgac gattgctatg gaaataccag
     5341 gtctgcaacg aaacgttgct tcgtccagct atagttttgt ccgctttttc ggtggagctt
     5401 tagcaccatt tattgcaggt gtgattggtg aacatattgg tgaaagctat gcgttttatt
     5461 ttgcaggtgc cattgttctt atcagtgtat gttgtatcat ttatcaccgt aattattttg
     5521 ctacaaatga agaagaataa gaaatcagag gttgtgaaaa agctgtcctg catcaagtca
     5581 taagaa
//
LOCUS       DAJSBN010000228         5105 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.guided.110, whole genome shotgun sequence.
ACCESSION   DAJSBN010000228 DAJSBN010000000
VERSION     DAJSBN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.guided.110"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..276
                     /locus_tag="ONT86_003055"
     CDS             <1..276
                     /locus_tag="ONT86_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR2353749.1"
                     /translation="TVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYHGS
                     AKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            511..747
                     /locus_tag="ONT86_003056"
     CDS             511..747
                     /locus_tag="ONT86_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353750.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            754..2706
                     /locus_tag="ONT86_003057"
     CDS             754..2706
                     /locus_tag="ONT86_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR2353751.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2778..3275)
                     /gene="dfrG"
                     /locus_tag="ONT86_003058"
     CDS             complement(2778..3275)
                     /gene="dfrG"
                     /locus_tag="ONT86_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR2353752.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3637..4677
                     /locus_tag="ONT86_003059"
     CDS             3637..4677
                     /locus_tag="ONT86_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2353753.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4775..4963
                     /locus_tag="ONT86_003060"
     CDS             4775..4963
                     /locus_tag="ONT86_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2353754.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(5021..>5105)
                     /locus_tag="ONT86_003061"
     CDS             complement(5021..>5105)
                     /locus_tag="ONT86_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2353755.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT         1781 a          819 c          913 g         1592 t
ORIGIN      
        1 acggtaatag agggaaacga tgacctttta gagaaatata tgtccggtaa atcattagaa
       61 gcattggaac tcgaacaaga ggaaagcata agatttcaga attgttctct gttccctctt
      121 tatcatggaa gtgcaaaaag taatataggg attgataacc ttatagaagt tattactaat
      181 aaattttatt catcaacaca tcgaggtccg tctgaacttt gcggaaatgt tttcaaaatt
      241 gaatatacaa aaaaaagaca acgtcttgca tattaagtag ttcagttttg gagtacaaaa
      301 agtgaaaatg taaacctaaa tgagtatcaa attgcaactg aatttgatac tcgtttttta
      361 ttgttaaatc aacgtttgtt gttaatttac accacaaaat gtaagttaaa atgtatataa
      421 aaatgcgaac gtgaaataat tattgtagga atgcaaattg gtgtgttaaa atggttacaa
      481 tttactaagc aaaaaggggt gcattttcct atgcaatttc ttgagccaaa aaataagaac
      541 gctaagtctg tggattggga aatatcagag caagtaaggg tgatagttaa gcaatacgct
      601 gaatatgcag agcgtactga aagcgaagca gtggatgaat tcttattgaa cattttagac
      661 gataagaagt ttattgaatg gattgccaac aagcgaagta ataagcggat tgttgaaaaa
      721 atgggtatca aagatagagt gggttgatag acaatgacca agcttaaaag attagccacg
      781 aaggaagatg aaattgtaga tgttaaaatt ccgatttcaa acgaagaatt aaaagatagg
      841 gctaaacaat atgatttatt aactccaaaa cgatttgcaa caaggtataa caaaatgttg
      901 ttcttgccaa cgtcttttaa atggaacggt tctgaatatc ccattcaata taattactgc
      961 atcaatccgt tttgttgtaa ttttgggaaa gaacagcaca aattcaaaga tgtaaaaggg
     1021 aaaccaagtc gatataaaat gactggtagt tccaaagata aaggtcataa aggtatgtac
     1081 tgcaatgaca atcctattgg cagaggtgta tcgcaaaact gtacagtaac ccctttatct
     1141 aattggtctg ttgttgaaga aattaaacgc cttatagaaa tcaatagcat ccaagatgtt
     1201 gagccagatt atcaatttca taaagaaggc tgctcggaag aagaatcaac cccattcaat
     1261 gagccgaagc aattttacaa aagggggaaa agtaggggca aatctcaacg ctatcaatgt
     1321 aaggcgtgta agaagttcac taatgttcta ccaaaaagag aagaaaccac tacttatcac
     1381 caacaaaaga acaccattct tccaatgttc gctaagatgg ttgttggaag ggtttcagtc
     1441 agtcgtactt gtgatatttt aggaattgga gttggaacct actatcataa gctggagtgg
     1501 ctgtataggc gttgtttgga gtttttagaa cgatatgaga cacaacccct tcaaacaaag
     1561 aaatttaacg agatgtggct taatacggac aagatgcact actacctaaa taacgtccgt
     1621 aagaaaggac aaggttcaaa gaaatatacg gggtttgaag accttaatat gcaaacgtat
     1681 atagttgtat ctgctgaggt cttatcaaga tatgtgttcc gttctgatgt tgcctatgat
     1741 tggaatattt caatggatga attaaatgaa gatacaagaa aattcaaaga agaccatctc
     1801 aatactttta gtcgtaaaaa tgataggtta gattggtctt attatccaca agaaccatcc
     1861 gctaacgata gcgaaaatag gaatgcttat cttcacgaat tagggaaaat tactaatagg
     1921 agtagatttg tcgatggttt aaatgttgat gctccttata ctacaactgc tcactattgg
     1981 ttaattaaac aaatggttaa tgccgacgaa tggcgaatga ttagtgatga tgacttctct
     2041 attagaaacg ccttttatag agtattcacg aaagaattaa ggttgagcga tgcacatcat
     2101 tttatttgcc aagttaacaa gacgaaaagc agaaaacaat gcttaaaaga attcggacaa
     2161 gcaaaagcag aactgctcga ttggggagat atcagaggat tcaaaacaaa gtttctacgg
     2221 actttggcat ctcattatct aactgaatta ctaacaagtc atcaattcca cgaagaggct
     2281 attagtaaag atggagaaag atatcgcaaa tatgccgata accctatcaa acatccatta
     2341 gccactaagg ataaaggatt ttattcagtt gattgtagaa cggatttatc tgctctggaa
     2401 ccaaacgaaa tcgcaaagat gctattaaat gttaatgacc attcgaccaa tagctttatt
     2461 caacagataa gacgttatat atcatctctt gaaagaccat taacaacagc tcgtggtgat
     2521 aagaagagct atatttacgc taacttcaat ccgaagtatg ctcaatttgc gattacaatt
     2581 ctaagaactt actacaactt ttgtcgccct tttaaatcag cggataaaaa ggttttgaca
     2641 cctgctcaaa gattagggat tacagataaa caatttgatt ggaaagatat aatttatttc
     2701 aaatgacaaa aagagcttgg agtaatccca agctcttgtg ctttatcttt actgttcttc
     2761 ttcaattaac gcacccgtta actcaataag ttttttcttt catatacatg aaaatagtag
     2821 ttatacggat ttttatcatt ctttatccct ttttgggcaa atacctcatt ccattcctca
     2881 taattcactt ctggaaaaaa agtatctcct tcgaattcat gatgtatttt tgtgatgtac
     2941 attttctcaa cataagggaa aaacaaatta taaatctgtt ctcctccgaa aataaaaatt
     3001 tcttcttcgt ttttacataa ctcaaaaaca tcttctattg aatgaacaat ttcacaacca
     3061 ttaaaggtaa accccttatc tctcgtcaga ataatatttc ttctgtcagg taaggctctt
     3121 ccgattgatt caaggttctt cctacctaat attatcggat gtccctttgt agtattttta
     3181 acatattccc agtccttggg aatcctccaa ggaatgtcat tctctttgcc aatcactcta
     3241 ttcttatcca tcgcagcaat caaagaaact ttcatttata acacagcctt tttaactaat
     3301 ttaccatcta atttcttatt tctatcaaaa aaccttttct cattatcctg cccgttagtt
     3361 gaataagcaa ttttttctat aagttatatt atcataatac cacaaaatca caaattttca
     3421 ttcaattatc attttcaatt gcattttata aatcattttg tatgtaattt ggtatgtaag
     3481 ttaaaatgaa aaaagtagtc cattttgaag tacattttgt cattttgtac tccaaaactg
     3541 aactacttac ttgcatatat acgcctttat agtggagtac tacatttacg agattcggtt
     3601 agagtatcag aaaaagaaaa aataaaagtt acagaaatgt atacttcaat aaatggtgaa
     3661 ttatgtaaga ttgatagagc ttattctgga gaaattgtta ttttgcaaaa tgagtttttg
     3721 aagttaaata gtgttcttgg agatacaaaa ctattgccac agagaaaaaa gattgaaaat
     3781 ccgcaccctc tactacaaac aactgttgaa ccgagtaaac ctgaacagag agaaatgttg
     3841 cttgatgccc ttttggaaat ctcagatagt gatccgcttc tacgatatta cgtggattct
     3901 acgacacatg aaattatact ttctttctta gggaaagtac aaatggaagt gattagtgca
     3961 ctgttgcaag aaaagtatca tgtggagata gaactaaaag agcctacagt catttatatg
     4021 gagagaccgt taaaaaatgc agaatatacc attcacatcg aagtgccgcc aaatcctttc
     4081 tgggcttcca ttggtttatc tgtatcaccg cttccgttgg gaagtggaat gcagtatgag
     4141 agctcggttt ctcttggata cttaaatcaa tcatttcaaa atgcagttat ggaagggata
     4201 cgctatggtt gcgaacaagg attatatggt tggaatgtga cggattgtaa aatctgtttt
     4261 aagtacggtt tatactatag ccctgttagt actccagcag attttcggat gcttactcct
     4321 attgtactgg agcaagcctt tagaaaagct ggaacagaat tgttagagcc atatcttagt
     4381 tttaaagttt atgcaccaca ggaatatctt tcacgggcat ataacgatgc tcccaaatat
     4441 tgtgcaaata tcgtaaatac tcaactgaaa aataatgagg tcattattat tggagaaatt
     4501 cctgctcgat gtattcaaga ttatcgcaat gatttaactt tttttacaaa tgggcttagt
     4561 gtttgtttag cagagctaaa aggatatcag gttaccactg gcgaacctgt ttgccagacc
     4621 cgtcgtctaa atagtcggat agataaagta agatatatgt tcaataaaat aacttagtgc
     4681 gttttatgtt gttatataaa tatggtttct tattaaataa gatgaaatat tctttaatat
     4741 agatttgaat taaagtggaa aggaggagat tgttattata aactacaagt ggatattgtg
     4801 tcctatttgt ggaaataaaa caagactacg aatacgagtg gatactatac ttaaaaattt
     4861 ccctttatac tgccccaaat gtaagaacga aactttaatt aatgttcaaa aaatgaatat
     4921 aataacaatc aaagagccag acgccaagac gcagagccga taatttgaga aatgaaactc
     4981 tcatcttatc ggctcttttt gtttatctga attttactga ctagccttca atatttcttg
     5041 cttcattgat accttttgct agtgcttcca ttaaggatag ttctttgtct gtaaagctat
     5101 ccatg
//
LOCUS       DAJSBN010000229         3113 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.guided.139, whole genome shotgun sequence.
ACCESSION   DAJSBN010000229 DAJSBN010000000
VERSION     DAJSBN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.guided.139"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            31..813
                     /locus_tag="ONT86_003062"
     CDS             31..813
                     /locus_tag="ONT86_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HCR2353756.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(939..1670)
                     /gene="erm(A)"
                     /locus_tag="ONT86_003063"
     CDS             complement(939..1670)
                     /gene="erm(A)"
                     /locus_tag="ONT86_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HCR2353757.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(1728..1787)
                     /locus_tag="ONT86_003064"
     CDS             complement(1728..1787)
                     /locus_tag="ONT86_003064"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HCR2353758.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            2179..2841
                     /locus_tag="ONT86_003065"
     CDS             2179..2841
                     /locus_tag="ONT86_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2353759.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         1031 a          453 c          571 g         1058 t
ORIGIN      
        1 tatgaacata atcagttacg aggtgaaatc atgagcaatt tgattaacgg aaaaatacca
       61 aatcaagcga ttcaaacatt aaaaatcgta aaagatttat ttggaagttc aatagttgga
      121 gtatatctat ttggttcagc agtaaatggt ggtttacgca ttaacagcga tgtagatgtt
      181 ctagtcgtcg tgaatcatag tttacctcaa ttaactcgaa aaaaactaac agaaagacta
      241 atgactatat caggaaagat tggaaatacg gattctgtta gaccacttga agttacggtt
      301 ataaatagga gtgaagttgt cccttggcaa tatcctccaa aaagagaatt tatatacggt
      361 gagtggctca ggggtgaatt tgagaatgga caaattcagg aaccaagcta tgatcctgat
      421 ttggctattg ttttagcaca agcaagaaag aatagtattt ctctatttgg tcctgattct
      481 tcaagtatac ttgtctccgt acctttgaca gatattcgaa gagcaattaa ggattctttg
      541 ccagaactaa ttgaggggat aaaaggtgat gagcgtaatg taattttaac cctagctcga
      601 atgtggcaaa cagtgactac tggtgaaatt acctcgaaag atgtcgctgc agaatgggct
      661 atacctcttt tacctaaaga gcatgtaact ttactggata tagctagaaa aggctatcgg
      721 ggagagtgtg atgataagtg ggaaggacta tattcaaagg tgaaagcact cgttaagtat
      781 atgaaaaatt ctatagaaac ttctctcaat taggctaatt ttattgcaat aacaggtgct
      841 tacttttaaa actactgatt tattgataaa tattgaacaa tttttgggaa gaataaagcg
      901 tcctcttgtg aaattagaga acgctttatt actttaattt agtgaaacaa tttgtaacta
      961 ttgaaaatag aaagaaattg ttccttcgat agtttattaa tattagtgac atttgcatgc
     1021 ttcaaagcct gtcggaattg gtttttagtg aaaagaacac gatattcacg gtttacccac
     1081 ttataaacaa aagatcgata ctttttgtag tccttctttg aaatcaatgg ttgatgtcgt
     1141 tcaagaacaa tcaatacaga gtctacactt ggcttaggat gaaaatatag tggtggtact
     1201 tttttgagca tttttatatc catctccacc attaatagta aacccaaagc tcgttgcaga
     1261 ttttgcaatc ttttcgcaaa tcccttctca acgataagat agctatattt agcctgactt
     1321 tcaaaggtaa ttcttttgac aatatccgta ctgatgttat aaggaatatt accatatatc
     1381 ttatagttta tatgttttgg gaaggaaaat tttagaatat ccgtttgaat cacttttata
     1441 ttctcagagg ggtttaccgc ttctttagtc acttgacata agcctccatc aatttctata
     1501 gcagtaactg atcgactcat tttgactagc tctttggtaa aatgtccttt tcctgatccg
     1561 atttctatta cgttgtcttg tttactgata ttcgtgtgat tcaatatttc ttttacatgc
     1621 tttttagaag taataaaatt ttgcgtgtct ttagggtttt tctggttcat tataaccttc
     1681 tccttactgg ttataatgaa ctgacattaa gcgttcatta taaccaatta attttgattt
     1741 ggttgataac gaactttatt aataacaaat atagaaaaag tacccatact tttatctcct
     1801 ttccattttt ttcattatga atgagataaa gtaatttcta ctactgcgat actggtgcac
     1861 ataattgaaa cctccttatg taactgaata tgattgtatc atcttttcat tttagaataa
     1921 acctttatat gtaattttgc acttgacact tcagatggac gttttcttca aaataatatg
     1981 ttttgaaact tgataagaga agccactgtc tttactaaaa agtaaaggct gcgataaagg
     2041 acagtgactt taataaagtt gcactgttgt attgtctttg gataaaattg tctaaaggga
     2101 agttttatcc ccaacgaaaa aacatctaaa gtggtaagta atgaaagaat gtcgataaag
     2161 taggaatggg ggatcttttt gaatacaata gaatcacagt ttgataaggt tgcagaagat
     2221 tacgatttcg tgaatgagct tttgaatgat tattcattct ttgtgtctaa tatgtctcca
     2281 aagaaaggca gagcattaga tatcggatgt ggctcgggtt tgttagtgga gaaattagca
     2341 agttattatg atgaagtggt agggattgat atttctaatc aaatgctcga tcttgccaaa
     2401 tctaaacgtc aactaacaaa tacggtctat ctgaatatga atgcagaaca acttaatttt
     2461 aatgagaagt ttgattttat tgtaagccga acaacctttc atcatttgga tgatatagcc
     2521 agcgtaatac agcaaatgaa ggaactgttg aatgaggaag gaagaatagt tattcttgat
     2581 aatgtatctg aagtcgagac accgcctacc tatgtttata aattaggggc aatccaagag
     2641 tttttaccgc attgtttcaa atttggaata aaaaatgcga ttagaattta taatcacaac
     2701 acatctaaat catggcttga gcatttagct tcagataagt acctatctga acaaaattat
     2761 tacgatttat atgaaaagtt gttgcctgga tgccaattcc ataaaatggg ttgggcaatg
     2821 ggggttgtct ggacaaaata acttaaagct gtggctttga aaagttgaat ttgtgattgt
     2881 tttcagagct atcgggtgct ttagtataaa taacaatgaa taaaaaatca gcctgtatct
     2941 tatgttacta ttaagtaatt taagatacag gttttactat ttttgaaata agtaccttcc
     3001 acttcaaata catgtattca aagtgaaagt attgatttaa atgattcttt tgtaatttac
     3061 tcttgataat atgaaattcc atcgcagcag gatatgaatt ttaccaaaag gat
//
LOCUS       DAJSBN010000230         2371 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.guided.155, whole genome shotgun sequence.
ACCESSION   DAJSBN010000230 DAJSBN010000000
VERSION     DAJSBN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.guided.155"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT86_003066"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT86_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2353760.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLDSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2060..2227
                     /locus_tag="ONT86_003067"
     CDS             2060..2227
                     /locus_tag="ONT86_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2353761.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2287..>2371)
                     /locus_tag="ONT86_003068"
     CDS             complement(2287..>2371)
                     /locus_tag="ONT86_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2353762.1"
                     /translation="MDSFTDKELSLMESLASGINEARNIED"
BASE COUNT          817 a          361 c          483 g          710 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta gatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaaata
     1261 aaagagccta cagtcattta tatggaaaga ccgttaaaaa aagcagagta taccattcac
     1321 atcgaagttc caccgaatcc tttctgggct tccattggtc tatctgtagc acagcttcca
     1381 ttagggagcg gagtacagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     1441 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     1501 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg agatagttat
     2041 tataaactac aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag
     2101 ggaagatact gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaattt
     2161 aattgaaata aagcagttca aagtaactgt gattacagag ccagacgcaa agacgcagag
     2221 ccgataaaat gagattaata caatctcatt ttatcggctc tttccgttat gtatggattc
     2281 ttttaattag tcttcgatgt ttcttgcttc gttgataccg ctggctaaag attccattaa
     2341 ggatagttct ttgtctgtaa agctatccat g
//
LOCUS       DAJSBN010000231         2371 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.guided.156, whole genome shotgun sequence.
ACCESSION   DAJSBN010000231 DAJSBN010000000
VERSION     DAJSBN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.guided.156"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT86_003069"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT86_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2353763.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLDSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2060..2227
                     /locus_tag="ONT86_003070"
     CDS             2060..2227
                     /locus_tag="ONT86_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2353764.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2287..>2371)
                     /locus_tag="ONT86_003071"
     CDS             complement(2287..>2371)
                     /locus_tag="ONT86_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2353765.1"
                     /translation="MDSFTDKELSLMESLASGINEARNIED"
BASE COUNT          816 a          361 c          484 g          710 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta gatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaaata
     1261 aaagagccta cagtcattta tatggaaaga ccgttaaaaa aagcagagta taccattcac
     1321 atcgaagttc caccgaatcc tttctgggct tccattggtc tatctgtagc acagcttcca
     1381 ttagggagcg gagtacagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     1441 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     1501 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg agatagttat
     2041 tataaactac aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag
     2101 ggaagatact gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaattt
     2161 aattgaaata aagcagttca aagtaactgt gattacagag ccagacgcaa agacgcagag
     2221 ccgataaaat gagattaata caatctcatt ttatcggctc tttccgttat gtatggattc
     2281 ttttaattag tcttcgatgt ttcttgcttc gttgataccg ctggctaaag attccattaa
     2341 ggatagttct ttgtctgtaa agctatccat g
//
LOCUS       DAJSBN010000232         1655 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.guided.168, whole genome shotgun sequence.
ACCESSION   DAJSBN010000232 DAJSBN010000000
VERSION     DAJSBN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.guided.168"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..290
                     /locus_tag="ONT86_003072"
     CDS             <1..290
                     /locus_tag="ONT86_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2353766.1"
                     /translation="KIIGFAYCYTLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFI
                     KEYSKEIGCSEMFLITDKGNPRACHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            291..>1655
                     /gene="aph(2'')-I"
                     /locus_tag="ONT86_003073"
     CDS             291..>1655
                     /gene="aph(2'')-I"
                     /locus_tag="ONT86_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR2353767.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          686 a          128 c          283 g          558 t
ORIGIN      
        1 ataaaattat aggatttgca tattgctata cacttttaag acctgatgga aaaacaatgt
       61 tttatttaca ctcaatagga atgttaccta actatcaaga caaaggttat ggttcaaaat
      121 tattatcttt tattaaggaa tattctaaag agattggttg ttctgaaatg tttttaataa
      181 ctgataaagg taatcctaga gcttgccatg tatatgaaaa attaggtggt aaaaatgatt
      241 ataaagatga aatagtatat gtatatgatt atgaaaaagg tgataaataa atgaatatag
      301 ttgaaaatga aatatgtata agaactttaa tagatgatga ttttcctttg atgttaaaat
      361 ggttaactga tgaaagagta ttagaatttt atgatggtag agataaaaaa tatacattag
      421 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
      481 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      541 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      601 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      661 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      721 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      781 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      841 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      901 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
      961 tttttaaaac aaaatttagt actaataaga aaaaaggtta tgcaaaagaa aaagcaatat
     1021 ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcctaatatt gaatattcgt
     1081 atattagtga tgaattatct atactaggtt ataaagaaat taaaggaact tttttaacac
     1141 cagaaattta ttctactatg tcagaagaag aacaaaattt gttaaaacga gatattgcca
     1201 gttttttaag acaaatgcac ggtttagatt atacagatat tagtgaatgt actattgata
     1261 ataaacaaaa tgtattagaa gagtatatat tgttgcgtga aactatttat aatgatttaa
     1321 ctgatataga aaaagattat atagaaagtt ttatggaaag actaaatgca acaacagttt
     1381 ttgagggtaa aaagtgttta tgccataatg attttagttg taatcatcta ttgttagatg
     1441 gcaataatag attaactgga ataattgatt ttggagattc tggaattata gatgaatatt
     1501 gtgattttat atacttactt gaagatagtg aagaagaaat aggaacaaat tttggagaag
     1561 atatattaag aatgtatgga aatatagata ttgagaaagc aaaagaatat caagatatag
     1621 ttgaagaata ttatcctatt gaaactattg tttat
//
LOCUS       DAJSBN010000233         1504 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.guided.176, whole genome shotgun sequence.
ACCESSION   DAJSBN010000233 DAJSBN010000000
VERSION     DAJSBN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.guided.176"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            262..345
                     /locus_tag="ONT86_003074"
                     /pseudo
     CDS             262..345
                     /locus_tag="ONT86_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="ONT86_003075"
     CDS             394..477
                     /locus_tag="ONT86_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCR2353768.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ONT86_003076"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ONT86_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR2353769.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAJSBN010000234         1382 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.guided.184, whole genome shotgun sequence.
ACCESSION   DAJSBN010000234 DAJSBN010000000
VERSION     DAJSBN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.guided.184"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1311
                     /locus_tag="ONT86_003077"
     CDS             <1..1311
                     /locus_tag="ONT86_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HCR2353770.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DAJSBN010000235         1350 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.guided.186, whole genome shotgun sequence.
ACCESSION   DAJSBN010000235 DAJSBN010000000
VERSION     DAJSBN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.guided.186"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            47..298
                     /locus_tag="ONT86_003078"
                     /pseudo
     CDS             47..298
                     /locus_tag="ONT86_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="ONT86_003079"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="ONT86_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCR2353771.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DAJSBN010000236          953 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-06
            SAMN30692149-rid15768453.guided.206, whole genome shotgun sequence.
ACCESSION   DAJSBN010000236 DAJSBN010000000
VERSION     DAJSBN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692149
            Sequence Read Archive: SRR21444928
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472587.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472587.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 22 of 73
            Pseudo Genes (incomplete)         :: 40 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692149-rid15768453.guided.206"
                     /isolate="VRED13-06"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            238..786
                     /gene="aac(6')"
                     /locus_tag="ONT86_003080"
     CDS             238..786
                     /gene="aac(6')"
                     /locus_tag="ONT86_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR2353772.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          337 a          142 c          202 g          272 t
ORIGIN      
        1 tttaaagcaa aagataatct atatcatgat gaacaaacag agttttttcc tccaattgat
       61 ggctttcctg atctttccta ttttgaataa agtgtttctg tataattttt ttattttata
      121 agtaagataa ataatgaaag aatagagata ctgattggta gctaaagatt tttgagcgag
      181 aacatttcgt tttttcaaaa ggactgttag aataagcaag aacaaggggg aagacgtatg
      241 ataatcagtg aatttgaccg taataatcca gtattgaaag atcagctttc tgatttactg
      301 agactgactt ggccggaaga atatggagac agctcggcag aagaagtaga agaaatgatg
      361 aatccagaac gaatcgcggt agcagcggta gaccaagatg agttagtagg atttattggt
      421 gcaatccctc aatacggtat cacaggttgg gaattgcatc cattagttgt agaaagctcc
      481 cgacgaaaga accaaatagg tactcgatta gtcaattact tagaaaaaga agtagcttcc
      541 agaggaggaa tcacgattta tttaggtacg gatgatttag accatggaac aacgttaagt
      601 caaacggacc tgtatgagca tacatttgat aaagtggctt ccatccagaa ccttcgtgaa
      661 catccgtatg aattctatga aaaattaggt tataaaatcg taggtgtctt accaaatgca
      721 aatggctggg acaaaccgga tatttggatg gcaaaaacga ttattcctcg accaaattct
      781 caataataaa aagaggttgt gacagaagta atcagcttca agaaataaga aggaatttac
      841 gaaaattact tcttgaattt ttgtgaaatt ccaacttatt tccgaagaag ctacttttgg
      901 aacaccgtgt accagaggtt gtgaaaaagt cgcttagttt cgagtagtaa gaa
//
